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PMC:1448208 / 29642-31350 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T16484 0-7 IN denotes Because
T16483 0-248 sentence denotes Because the LAP-tTA mice that were used to drive expression of transgenic human E2 have been demonstrated to produce liver specific expression [34], BCKDH enzyme activity and production of human E2 protein in liver of Lines A and 525A was examined.
T16486 8-11 DT denotes the
T16488 12-15 NN denotes LAP
T16490 15-16 HYPH denotes -
T16489 16-19 NN denotes tTA
T16487 20-24 NNS denotes mice
T16491 25-29 WDT denotes that
T16493 30-34 VBD denotes were
T16492 35-39 VBN denotes used
T16494 40-42 TO denotes to
T16495 43-48 VB denotes drive
T16496 49-59 NN denotes expression
T16497 60-62 IN denotes of
T16498 63-73 JJ denotes transgenic
T16500 74-79 JJ denotes human
T16499 80-82 NN denotes E2
T16501 83-87 VBP denotes have
T16502 88-92 VBN denotes been
T16485 93-105 VBN denotes demonstrated
T16504 106-108 TO denotes to
T16505 109-116 VB denotes produce
T16506 117-122 NN denotes liver
T16507 123-131 JJ denotes specific
T16508 132-142 NN denotes expression
T16509 143-144 -LRB- denotes [
T16510 144-146 CD denotes 34
T16511 146-147 -RRB- denotes ]
T16512 147-149 , denotes ,
T16513 149-154 NN denotes BCKDH
T16515 155-161 NN denotes enzyme
T16514 162-170 NN denotes activity
T16516 171-174 CC denotes and
T16517 175-185 NN denotes production
T16518 186-188 IN denotes of
T16519 189-194 JJ denotes human
T16521 195-197 NN denotes E2
T16520 198-205 NN denotes protein
T16522 206-208 IN denotes in
T16523 209-214 NN denotes liver
T16524 215-217 IN denotes of
T16525 218-223 NNS denotes Lines
T16526 224-225 NN denotes A
T16527 226-229 CC denotes and
T16528 230-234 NN denotes 525A
T16529 235-238 VBD denotes was
T16503 239-247 VBN denotes examined
T16530 247-248 . denotes .
T16531 248-395 sentence denotes BCKDH enzymatic activity in liver from Lines A and 525A was ~6 and 5%, respectively, of the enzymatic activity present in control liver (Fig. 4C).
T16532 249-254 NN denotes BCKDH
T16534 255-264 JJ denotes enzymatic
T16533 265-273 NN denotes activity
T16536 274-276 IN denotes in
T16537 277-282 NN denotes liver
T16538 283-287 IN denotes from
T16539 288-293 NNS denotes Lines
T16540 294-295 NN denotes A
T16541 296-299 CC denotes and
T16542 300-304 NN denotes 525A
T16535 305-308 VBD denotes was
T16543 309-310 SYM denotes ~
T16544 310-311 CD denotes 6
T16546 312-315 CC denotes and
T16547 316-317 CD denotes 5
T16545 317-318 NN denotes %
T16548 318-320 , denotes ,
T16549 320-332 RB denotes respectively
T16550 332-334 , denotes ,
T16551 334-336 IN denotes of
T16552 337-340 DT denotes the
T16554 341-350 JJ denotes enzymatic
T16553 351-359 NN denotes activity
T16555 360-367 JJ denotes present
T16556 368-370 IN denotes in
T16557 371-378 NN denotes control
T16558 379-384 NN denotes liver
T16559 385-386 -LRB- denotes (
T16561 386-390 NN denotes Fig.
T16560 391-393 NN denotes 4C
T16562 393-394 -RRB- denotes )
T16563 394-395 . denotes .
T16564 395-518 sentence denotes As shown in Fig. 3B, the amount of human E2 (predicted MW~54 Kd) in these transgenic mice was quite variable between mice.
T16565 396-398 IN denotes As
T16566 399-404 VBN denotes shown
T16568 405-407 IN denotes in
T16569 408-412 NNP denotes Fig.
T16570 413-415 NN denotes 3B
T16571 415-417 , denotes ,
T16572 417-420 DT denotes the
T16573 421-427 NN denotes amount
T16574 428-430 IN denotes of
T16575 431-436 JJ denotes human
T16576 437-439 NN denotes E2
T16577 440-441 -LRB- denotes (
T16579 441-450 VBN denotes predicted
T16580 451-453 NN denotes MW
T16581 453-454 SYM denotes ~
T16582 454-456 NN denotes 54
T16578 457-459 NN denotes Kd
T16583 459-460 -RRB- denotes )
T16584 461-463 IN denotes in
T16585 464-469 DT denotes these
T16587 470-480 JJ denotes transgenic
T16586 481-485 NNS denotes mice
T16567 486-489 VBD denotes was
T16588 490-495 RB denotes quite
T16589 496-504 JJ denotes variable
T16590 505-512 IN denotes between
T16591 513-517 NNS denotes mice
T16592 517-518 . denotes .
T16593 518-682 sentence denotes In some of these transgenic mice, the level of human E2 was approximately equal to the amount of mouse E2 (~47 Kd) produced in liver of nontransgenic control mice.
T16594 519-521 IN denotes In
T16596 522-526 DT denotes some
T16597 527-529 IN denotes of
T16598 530-535 DT denotes these
T16600 536-546 JJ denotes transgenic
T16599 547-551 NNS denotes mice
T16601 551-553 , denotes ,
T16602 553-556 DT denotes the
T16603 557-562 NN denotes level
T16604 563-565 IN denotes of
T16605 566-571 JJ denotes human
T16606 572-574 NN denotes E2
T16595 575-578 VBD denotes was
T16607 579-592 RB denotes approximately
T16608 593-598 JJ denotes equal
T16609 599-601 IN denotes to
T16610 602-605 DT denotes the
T16611 606-612 NN denotes amount
T16612 613-615 IN denotes of
T16613 616-621 NN denotes mouse
T16614 622-624 NN denotes E2
T16615 625-626 -LRB- denotes (
T16617 626-627 SYM denotes ~
T16618 627-629 CD denotes 47
T16616 630-632 NN denotes Kd
T16619 632-633 -RRB- denotes )
T16620 634-642 VBN denotes produced
T16621 643-645 IN denotes in
T16622 646-651 NN denotes liver
T16623 652-654 IN denotes of
T16624 655-668 JJ denotes nontransgenic
T16626 669-676 NN denotes control
T16625 677-681 NNS denotes mice
T16627 681-682 . denotes .
T16628 682-869 sentence denotes The observations that these near normal amounts of E2 protein result in only ~5–6% of normal BCKDH enzyme activity suggest that transgene derived E2 is functioning at a suboptimal level.
T16629 683-686 DT denotes The
T16630 687-699 NNS denotes observations
T16632 700-704 IN denotes that
T16634 705-710 DT denotes these
T16636 711-715 RB denotes near
T16637 716-722 JJ denotes normal
T16635 723-730 NNS denotes amounts
T16638 731-733 IN denotes of
T16639 734-736 NN denotes E2
T16640 737-744 NN denotes protein
T16633 745-751 VBP denotes result
T16641 752-754 IN denotes in
T16642 755-759 RB denotes only
T16644 760-761 SYM denotes ~
T16645 761-762 CD denotes 5
T16646 762-763 SYM denotes –
T16643 763-764 CD denotes 6
T16647 764-765 NN denotes %
T16648 766-768 IN denotes of
T16649 769-775 JJ denotes normal
T16651 776-781 NN denotes BCKDH
T16652 782-788 NN denotes enzyme
T16650 789-797 NN denotes activity
T16631 798-805 VBP denotes suggest
T16653 806-810 IN denotes that
T16655 811-820 NN denotes transgene
T16657 821-828 VBN denotes derived
T16656 829-831 NN denotes E2
T16658 832-834 VBZ denotes is
T16654 835-846 VBG denotes functioning
T16659 847-849 IN denotes at
T16660 850-851 DT denotes a
T16662 852-862 JJ denotes suboptimal
T16661 863-868 NN denotes level
T16663 868-869 . denotes .
T16664 869-997 sentence denotes It is probable that the c-myc tag at the carboxy terminus of the transgene derived human E2 interfered with enzymatic activity.
T16665 870-872 PRP denotes It
T16666 873-875 VBZ denotes is
T16667 876-884 JJ denotes probable
T16668 885-889 IN denotes that
T16670 890-893 DT denotes the
T16672 894-895 NN denotes c
T16674 895-896 HYPH denotes -
T16673 896-899 NN denotes myc
T16671 900-903 NN denotes tag
T16675 904-906 IN denotes at
T16676 907-910 DT denotes the
T16678 911-918 NN denotes carboxy
T16677 919-927 NN denotes terminus
T16679 928-930 IN denotes of
T16680 931-934 DT denotes the
T16682 935-944 NN denotes transgene
T16683 945-952 VBN denotes derived
T16684 953-958 JJ denotes human
T16681 959-961 NN denotes E2
T16669 962-972 VBD denotes interfered
T16685 973-977 IN denotes with
T16686 978-987 JJ denotes enzymatic
T16687 988-996 NN denotes activity
T16688 996-997 . denotes .
T16689 997-1335 sentence denotes This interpretation is consistent with previous studies which have revealed that the carboxy terminus of an analogous subunit of the pyruvate dehydrogenase complex is essential for subunit interactions [35] and insertion of a Hisx6 tag on the carboxy terminus of an analogous E. coli subunit interfered with normal subunit assembly [36].
T16690 998-1002 DT denotes This
T16691 1003-1017 NN denotes interpretation
T16692 1018-1020 VBZ denotes is
T16693 1021-1031 JJ denotes consistent
T16694 1032-1036 IN denotes with
T16695 1037-1045 JJ denotes previous
T16696 1046-1053 NNS denotes studies
T16697 1054-1059 WDT denotes which
T16699 1060-1064 VBP denotes have
T16698 1065-1073 VBN denotes revealed
T16700 1074-1078 IN denotes that
T16702 1079-1082 DT denotes the
T16704 1083-1090 NN denotes carboxy
T16703 1091-1099 NN denotes terminus
T16705 1100-1102 IN denotes of
T16706 1103-1105 DT denotes an
T16708 1106-1115 JJ denotes analogous
T16707 1116-1123 NN denotes subunit
T16709 1124-1126 IN denotes of
T16710 1127-1130 DT denotes the
T16712 1131-1139 NN denotes pyruvate
T16713 1140-1153 NN denotes dehydrogenase
T16711 1154-1161 NN denotes complex
T16701 1162-1164 VBZ denotes is
T16714 1165-1174 JJ denotes essential
T16715 1175-1178 IN denotes for
T16716 1179-1186 NN denotes subunit
T16717 1187-1199 NNS denotes interactions
T16718 1200-1201 -LRB- denotes [
T16719 1201-1203 CD denotes 35
T16720 1203-1204 -RRB- denotes ]
T16721 1205-1208 CC denotes and
T16722 1209-1218 NN denotes insertion
T16724 1219-1221 IN denotes of
T16725 1222-1223 DT denotes a
T16727 1224-1229 NN denotes Hisx6
T16726 1230-1233 NN denotes tag
T16728 1234-1236 IN denotes on
T16729 1237-1240 DT denotes the
T16731 1241-1248 NN denotes carboxy
T16730 1249-1257 NN denotes terminus
T16732 1258-1260 IN denotes of
T16733 1261-1263 DT denotes an
T16735 1264-1273 JJ denotes analogous
T16736 1274-1276 FW denotes E.
T16737 1277-1281 FW denotes coli
T16734 1282-1289 NN denotes subunit
T16723 1290-1300 VBD denotes interfered
T16738 1301-1305 IN denotes with
T16739 1306-1312 JJ denotes normal
T16741 1313-1320 NN denotes subunit
T16740 1321-1329 NN denotes assembly
T16742 1330-1331 -LRB- denotes [
T16743 1331-1333 CD denotes 36
T16744 1333-1334 -RRB- denotes ]
T16745 1334-1335 . denotes .
T16746 1335-1440 sentence denotes It is also possible that human E2 was not fully functional when complexed with mouse E1 and E3 subunits.
T16747 1336-1338 PRP denotes It
T16748 1339-1341 VBZ denotes is
T16749 1342-1346 RB denotes also
T16750 1347-1355 JJ denotes possible
T16751 1356-1360 IN denotes that
T16753 1361-1366 JJ denotes human
T16754 1367-1369 NN denotes E2
T16752 1370-1373 VBD denotes was
T16755 1374-1377 RB denotes not
T16756 1378-1383 RB denotes fully
T16757 1384-1394 JJ denotes functional
T16758 1395-1399 WRB denotes when
T16759 1400-1409 VBN denotes complexed
T16760 1410-1414 IN denotes with
T16761 1415-1420 NN denotes mouse
T16762 1421-1423 NN denotes E1
T16763 1424-1427 CC denotes and
T16764 1428-1430 NN denotes E3
T16765 1431-1439 NNS denotes subunits
T16766 1439-1440 . denotes .
T16767 1440-1507 sentence denotes Human E2 shares ~88% identity to mouse E2 at the amino acid level.
T16768 1441-1446 JJ denotes Human
T16769 1447-1449 NN denotes E2
T16770 1450-1456 VBZ denotes shares
T16771 1457-1458 SYM denotes ~
T16772 1458-1460 CD denotes 88
T16773 1460-1461 NN denotes %
T16774 1462-1470 NN denotes identity
T16775 1471-1473 IN denotes to
T16776 1474-1479 NN denotes mouse
T16777 1480-1482 NN denotes E2
T16778 1483-1485 IN denotes at
T16779 1486-1489 DT denotes the
T16781 1490-1495 NN denotes amino
T16782 1496-1500 NN denotes acid
T16780 1501-1506 NN denotes level
T16783 1506-1507 . denotes .
T16784 1507-1613 sentence denotes We also used western blot analysis to analyze expression of the E2 transgene in brain, kidney and muscle.
T16785 1508-1510 PRP denotes We
T16787 1511-1515 RB denotes also
T16786 1516-1520 VBD denotes used
T16788 1521-1528 NNP denotes western
T16789 1529-1533 NN denotes blot
T16790 1534-1542 NN denotes analysis
T16791 1543-1545 TO denotes to
T16792 1546-1553 VB denotes analyze
T16793 1554-1564 NN denotes expression
T16794 1565-1567 IN denotes of
T16795 1568-1571 DT denotes the
T16797 1572-1574 NN denotes E2
T16796 1575-1584 NN denotes transgene
T16798 1585-1587 IN denotes in
T16799 1588-1593 NN denotes brain
T16800 1593-1595 , denotes ,
T16801 1595-1601 NN denotes kidney
T16802 1602-1605 CC denotes and
T16803 1606-1612 NN denotes muscle
T16804 1612-1613 . denotes .
T16805 1613-1708 sentence denotes As shown in Figure 3C, D and 3E respectively, expression of E2 is negligible in those tissues.
T16806 1614-1616 IN denotes As
T16807 1617-1622 VBN denotes shown
T16809 1623-1625 IN denotes in
T16810 1626-1632 NN denotes Figure
T16811 1633-1635 NN denotes 3C
T16812 1635-1637 , denotes ,
T16813 1637-1638 NN denotes D
T16814 1639-1642 CC denotes and
T16815 1643-1645 NN denotes 3E
T16816 1646-1658 RB denotes respectively
T16817 1658-1660 , denotes ,
T16818 1660-1670 NN denotes expression
T16819 1671-1673 IN denotes of
T16820 1674-1676 NN denotes E2
T16808 1677-1679 VBZ denotes is
T16821 1680-1690 JJ denotes negligible
T16822 1691-1693 IN denotes in
T16823 1694-1699 DT denotes those
T16824 1700-1707 NNS denotes tissues
T16825 1707-1708 . denotes .
R4388 T16484 T16485 mark Because,demonstrated
R4390 T16485 T16503 advcl demonstrated,examined
R4391 T16486 T16487 det the,mice
R4393 T16487 T16485 nsubjpass mice,demonstrated
R4394 T16488 T16489 compound LAP,tTA
R4395 T16489 T16487 compound tTA,mice
R4397 T16490 T16489 punct -,tTA
R4398 T16491 T16492 dep that,used
R4399 T16492 T16487 relcl used,mice
R4401 T16493 T16492 auxpass were,used
R4402 T16494 T16495 aux to,drive
R4403 T16495 T16492 advcl drive,used
R4404 T16496 T16495 dobj expression,drive
R4405 T16497 T16496 prep of,expression
R4407 T16498 T16499 amod transgenic,E2
R4408 T16499 T16497 pobj E2,of
R4409 T16500 T16499 amod human,E2
R4411 T16501 T16485 aux have,demonstrated
R4415 T16502 T16485 auxpass been,demonstrated
R4417 T16504 T16505 aux to,produce
R4418 T16505 T16485 xcomp produce,demonstrated
R4419 T16506 T16507 npadvmod liver,specific
R4421 T16507 T16508 amod specific,expression
R4422 T16508 T16505 dobj expression,produce
R4423 T16509 T16510 punct [,34
R4425 T16510 T16485 parataxis 34,demonstrated
R4426 T16511 T16510 punct ],34
R4427 T16512 T16503 punct ", ",examined
R4429 T16513 T16514 compound BCKDH,activity
R4430 T16514 T16503 nsubjpass activity,examined
R4431 T16515 T16514 compound enzyme,activity
R4433 T16516 T16514 cc and,activity
R4434 T16517 T16514 conj production,activity
R4435 T16518 T16517 prep of,production
R4437 T16519 T16520 amod human,protein
R4438 T16520 T16518 pobj protein,of
R4439 T16521 T16520 compound E2,protein
R4441 T16522 T16514 prep in,activity
R4442 T16523 T16522 pobj liver,in
R4443 T16524 T16523 prep of,liver
R4445 T16525 T16526 compound Lines,A
R4446 T16526 T16524 pobj A,of
R4447 T16527 T16526 cc and,A
R4449 T16528 T16526 conj 525A,A
R4450 T16529 T16503 auxpass was,examined
R4452 T16530 T16503 punct .,examined
R4455 T16532 T16533 nmod BCKDH,activity
R4456 T16533 T16535 nsubj activity,was
R4457 T16534 T16533 amod enzymatic,activity
R4459 T16536 T16533 prep in,activity
R4460 T16537 T16536 pobj liver,in
R4461 T16538 T16537 prep from,liver
R4462 T16539 T16540 compound Lines,A
R4464 T16540 T16538 pobj A,from
R4465 T16541 T16540 cc and,A
R4467 T16542 T16540 conj 525A,A
R4468 T16543 T16544 punct ~,6
R4469 T16544 T16545 nummod 6,%
R4471 T16545 T16535 attr %,was
R4472 T16546 T16544 cc and,6
R4474 T16547 T16544 conj 5,6
R4475 T16548 T16535 punct ", ",was
R4476 T16549 T16535 advmod respectively,was
R4478 T16550 T16535 punct ", ",was
R4479 T16551 T16535 prep of,was
R4480 T16552 T16553 det the,activity
R4482 T16553 T16551 pobj activity,of
R4483 T16554 T16553 amod enzymatic,activity
R4484 T16555 T16553 amod present,activity
R4486 T16556 T16555 prep in,present
R4487 T16557 T16558 compound control,liver
R4488 T16558 T16556 pobj liver,in
R4489 T16559 T16560 punct (,4C
R4490 T16560 T16535 parataxis 4C,was
R4492 T16561 T16560 compound Fig.,4C
R4493 T16562 T16560 punct ),4C
R4494 T16563 T16535 punct .,was
R4496 T16565 T16566 mark As,shown
R4498 T16566 T16567 advcl shown,was
R4499 T16568 T16566 prep in,shown
R4501 T16569 T16570 compound Fig.,3B
R4502 T16570 T16568 pobj 3B,in
R4503 T16571 T16567 punct ", ",was
R4505 T16572 T16573 det the,amount
R4506 T16573 T16567 nsubj amount,was
R4507 T16574 T16573 prep of,amount
R4508 T16575 T16576 amod human,E2
R4509 T16576 T16574 pobj E2,of
R4511 T16577 T16578 punct (,Kd
R4512 T16578 T16576 parataxis Kd,E2
R4513 T16579 T16580 amod predicted,MW
R4514 T16580 T16578 nsubj MW,Kd
R4516 T16581 T16582 punct ~,54
R4517 T16582 T16578 nummod 54,Kd
R4518 T16583 T16578 punct ),Kd
R4520 T16584 T16573 prep in,amount
R4521 T16585 T16586 det these,mice
R4522 T16586 T16584 pobj mice,in
R4524 T16587 T16586 amod transgenic,mice
R4525 T16588 T16589 advmod quite,variable
R4526 T16589 T16567 acomp variable,was
R4528 T16590 T16589 prep between,variable
R4531 T16591 T16590 pobj mice,between
R4532 T16592 T16567 punct .,was
R4534 T16594 T16595 prep In,was
R4535 T16596 T16594 pobj some,In
R4536 T16597 T16596 prep of,some
R4538 T16598 T16599 det these,mice
R4539 T16599 T16597 pobj mice,of
R4541 T16600 T16599 amod transgenic,mice
R4542 T16601 T16595 punct ", ",was
R4543 T16602 T16603 det the,level
R4545 T16603 T16595 nsubj level,was
R4546 T16604 T16603 prep of,level
R4547 T16605 T16606 amod human,E2
R4549 T16606 T16604 pobj E2,of
R4550 T16607 T16608 advmod approximately,equal
R4554 T16608 T16595 acomp equal,was
R4555 T16609 T16608 prep to,equal
R4557 T16610 T16611 det the,amount
R4558 T16611 T16609 pobj amount,to
R4559 T16612 T16611 prep of,amount
R4560 T16613 T16614 compound mouse,E2
R4561 T16614 T16612 pobj E2,of
R4562 T16615 T16616 punct (,Kd
R4564 T16616 T16614 parataxis Kd,E2
R4566 T16617 T16618 punct ~,47
R4567 T16618 T16616 nummod 47,Kd
R4569 T16619 T16616 punct ),Kd
R4570 T16620 T16611 acl produced,amount
R4571 T16621 T16620 prep in,produced
R4572 T16622 T16621 pobj liver,in
R4573 T16623 T16622 prep of,liver
R4575 T16624 T16625 amod nontransgenic,mice
R4576 T16625 T16623 pobj mice,of
R4578 T16626 T16625 compound control,mice
R4579 T16627 T16595 punct .,was
R4581 T16629 T16630 det The,observations
R4582 T16630 T16631 nsubj observations,suggest
R4584 T16632 T16633 mark that,result
R4585 T16633 T16630 acl result,observations
R4587 T16634 T16635 det these,amounts
R4588 T16635 T16633 nsubj amounts,result
R4589 T16636 T16637 advmod near,normal
R4591 T16637 T16635 amod normal,amounts
R4592 T16638 T16635 prep of,amounts
R4594 T16639 T16640 compound E2,protein
R4595 T16640 T16638 pobj protein,of
R4596 T16641 T16633 prep in,result
R4598 T16642 T16643 advmod only,6
R4599 T16643 T16647 nummod 6,%
R4600 T16644 T16643 punct ~,6
R4602 T16645 T16643 quantmod 5,6
R4603 T16646 T16643 punct –,6
R4604 T16647 T16641 pobj %,in
R4606 T16648 T16647 prep of,%
R4607 T16649 T16650 amod normal,activity
R4608 T16650 T16648 pobj activity,of
R4610 T16651 T16650 compound BCKDH,activity
R4611 T16652 T16650 compound enzyme,activity
R4612 T16653 T16654 mark that,functioning
R4613 T16654 T16631 ccomp functioning,suggest
R4614 T16655 T16656 nmod transgene,E2
R4615 T16656 T16654 nsubj E2,functioning
R4617 T16657 T16656 amod derived,E2
R4618 T16658 T16654 aux is,functioning
R4619 T16659 T16654 prep at,functioning
R4621 T16660 T16661 det a,level
R4622 T16661 T16659 pobj level,at
R4624 T16662 T16661 amod suboptimal,level
R4625 T16663 T16631 punct .,suggest
R4627 T16665 T16666 nsubj It,is
R4628 T16667 T16666 acomp probable,is
R4630 T16668 T16669 mark that,interfered
R4631 T16669 T16666 ccomp interfered,is
R4632 T16670 T16671 det the,tag
R4634 T16671 T16669 nsubj tag,interfered
R4635 T16672 T16673 compound c,myc
R4637 T16673 T16671 compound myc,tag
R4638 T16674 T16673 punct -,myc
R4639 T16675 T16671 prep at,tag
R4641 T16676 T16677 det the,terminus
R4642 T16677 T16675 pobj terminus,at
R4643 T16678 T16677 compound carboxy,terminus
R4645 T16679 T16677 prep of,terminus
R4646 T16680 T16681 det the,E2
R4647 T16681 T16679 pobj E2,of
R4649 T16682 T16683 npadvmod transgene,derived
R4650 T16683 T16681 amod derived,E2
R4651 T16684 T16681 amod human,E2
R4653 T16685 T16669 prep with,interfered
R4654 T16686 T16687 amod enzymatic,activity
R4655 T16687 T16685 pobj activity,with
R4657 T16688 T16666 punct .,is
R4659 T16690 T16691 det This,interpretation
R4660 T16691 T16692 nsubj interpretation,is
R4662 T16693 T16692 acomp consistent,is
R4663 T16694 T16693 prep with,consistent
R4664 T16695 T16696 amod previous,studies
R4665 T16696 T16694 pobj studies,with
R4666 T16697 T16698 dep which,revealed
R4668 T16698 T16696 relcl revealed,studies
R4669 T16699 T16698 aux have,revealed
R4670 T16700 T16701 mark that,is
R4673 T16701 T16698 ccomp is,revealed
R4674 T16702 T16703 det the,terminus
R4675 T16714 T16701 acomp essential,is
R4676 T16703 T16701 nsubj terminus,is
R4677 T16704 T16703 compound carboxy,terminus
R4678 T16705 T16703 prep of,terminus
R4679 T16706 T16707 det an,subunit
R4680 T16707 T16705 pobj subunit,of
R4681 T16708 T16707 amod analogous,subunit
R4682 T16715 T16714 prep for,essential
R4683 T16709 T16707 prep of,subunit
R4684 T16710 T16711 det the,complex
R4685 T16711 T16709 pobj complex,of
R4686 T16716 T16717 compound subunit,interactions
R4687 T16712 T16711 compound pyruvate,complex
R4688 T16713 T16711 compound dehydrogenase,complex
R4689 T16717 T16715 pobj interactions,for
R4690 T16718 T16719 punct [,35
R4691 T16719 T16701 parataxis 35,is
R4692 T16820 T16819 pobj E2,of
R4693 T16821 T16808 acomp negligible,is
R4694 T16720 T16719 punct ],35
R4695 T16822 T16808 prep in,is
R4696 T16823 T16824 det those,tissues
R4697 T16824 T16822 pobj tissues,in
R4698 T16721 T16701 cc and,is
R4699 T16825 T16808 punct .,is
R4700 T16722 T16723 nsubj insertion,interfered
R4704 T16723 T16701 conj interfered,is
R4706 T16724 T16722 prep of,insertion
R4710 T16725 T16726 det a,tag
R4717 T16726 T16724 pobj tag,of
R4721 T16727 T16726 compound Hisx6,tag
R4725 T16728 T16722 prep on,insertion
R4728 T16729 T16730 det the,terminus
R4730 T16730 T16728 pobj terminus,on
R4735 T16731 T16730 compound carboxy,terminus
R4739 T16732 T16730 prep of,terminus
R4742 T16733 T16734 det an,subunit
R4745 T16734 T16732 pobj subunit,of
R4749 T16735 T16734 amod analogous,subunit
R4753 T16736 T16737 nmod E.,coli
R4757 T16737 T16734 nmod coli,subunit
R4760 T16738 T16723 prep with,interfered
R4762 T16739 T16740 amod normal,assembly
R4766 T16740 T16738 pobj assembly,with
R4769 T16741 T16740 compound subunit,assembly
R4773 T16742 T16743 punct [,36
R4777 T16743 T16723 parataxis 36,interfered
R4780 T16744 T16743 punct ],36
R4784 T16745 T16692 punct .,is
R4787 T16747 T16748 nsubj It,is
R4791 T16749 T16748 advmod also,is
R4795 T16750 T16748 acomp possible,is
R4799 T16751 T16752 mark that,was
R4802 T16752 T16748 ccomp was,is
R4804 T16753 T16754 amod human,E2
R4808 T16754 T16752 nsubj E2,was
R4812 T16755 T16752 neg not,was
R4816 T16756 T16757 advmod fully,functional
R4822 T16757 T16752 acomp functional,was
R4824 T16758 T16759 advmod when,complexed
R4825 T16759 T16752 advcl complexed,was
R4826 T16760 T16759 prep with,complexed
R4829 T16761 T16762 compound mouse,E1
R4831 T16762 T16760 pobj E1,with
R4835 T16763 T16762 cc and,E1
R4837 T16764 T16765 compound E3,subunits
R4841 T16765 T16762 conj subunits,E1
R4843 T16766 T16748 punct .,is
R4848 T16768 T16769 amod Human,E2
R4851 T16769 T16770 nsubj E2,shares
R4854 T16771 T16772 punct ~,88
R4859 T16772 T16773 nummod 88,%
R4863 T16773 T16774 compound %,identity
R4867 T16774 T16770 dobj identity,shares
R4873 T16775 T16774 prep to,identity
R4876 T16776 T16777 compound mouse,E2
R4877 T16777 T16775 pobj E2,to
R4879 T16778 T16770 prep at,shares
R4883 T16779 T16780 det the,level
R4886 T16780 T16778 pobj level,at
R4890 T16781 T16782 compound amino,acid
R4893 T16782 T16780 compound acid,level
R4896 T16783 T16770 punct .,shares
R4898 T16785 T16786 nsubj We,used
R4903 T16787 T16786 advmod also,used
R4907 T16788 T16789 compound western,blot
R4908 T16789 T16790 compound blot,analysis
R4910 T16790 T16786 dobj analysis,used
R4914 T16791 T16792 aux to,analyze
R4918 T16792 T16786 advcl analyze,used
R4925 T16793 T16792 dobj expression,analyze
R4929 T16794 T16793 prep of,expression
R4931 T16795 T16796 det the,transgene
R4935 T16796 T16794 pobj transgene,of
R4939 T16797 T16796 compound E2,transgene
R4942 T16798 T16792 prep in,analyze
R4946 T16799 T16798 pobj brain,in
R4950 T16800 T16799 punct ", ",brain
R4951 T16801 T16799 conj kidney,brain
R4952 T16802 T16801 cc and,kidney
R4953 T16803 T16801 conj muscle,kidney
R4954 T16804 T16786 punct .,used
R4955 T16806 T16807 mark As,shown
R4956 T16807 T16808 advcl shown,is
R4957 T16809 T16807 prep in,shown
R4958 T16810 T16811 compound Figure,3C
R4959 T16811 T16809 pobj 3C,in
R4960 T16812 T16811 punct ", ",3C
R4961 T16813 T16811 conj D,3C
R4962 T16814 T16813 cc and,D
R4963 T16815 T16813 conj 3E,D
R4964 T16816 T16807 advmod respectively,shown
R4965 T16817 T16808 punct ", ",is
R4966 T16818 T16808 nsubj expression,is
R4967 T16819 T16818 prep of,expression