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PMC:1448208 / 23838-25123 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T23655 10-20 JJ denotes Transgenic
T23657 21-26 NN denotes mouse
T23656 27-37 NN denotes production
T23658 38-41 CC denotes and
T23659 42-58 NN denotes characterization
T23660 58-59 . denotes .
T23661 59-126 sentence denotes A, Transgenic strategy used to produce mice that express human E2.
T23662 60-61 LS denotes A
T23664 61-63 , denotes ,
T23665 63-73 JJ denotes Transgenic
T23663 74-82 NN denotes strategy
T23666 83-87 VBN denotes used
T23667 88-90 TO denotes to
T23668 91-98 VB denotes produce
T23669 99-103 NNS denotes mice
T23670 104-108 WDT denotes that
T23671 109-116 VBP denotes express
T23672 117-122 JJ denotes human
T23673 123-125 NN denotes E2
T23674 125-126 . denotes .
T23675 126-187 sentence denotes LAP-tTA transgenic mice have been previously described [34].
T23676 127-130 NN denotes LAP
T23678 130-131 HYPH denotes -
T23677 131-134 NN denotes tTA
T23680 135-145 JJ denotes transgenic
T23679 146-150 NNS denotes mice
T23682 151-155 VBP denotes have
T23683 156-160 VBN denotes been
T23684 161-171 RB denotes previously
T23681 172-181 VBN denotes described
T23685 182-183 -LRB- denotes [
T23686 183-185 CD denotes 34
T23687 185-186 -RRB- denotes ]
T23688 186-187 . denotes .
T23689 187-293 sentence denotes These mice express the tetracycline-controlled transactivator (tTA) from the liver specific LAP promoter.
T23690 188-193 DT denotes These
T23691 194-198 NNS denotes mice
T23692 199-206 VBP denotes express
T23693 207-210 DT denotes the
T23695 211-223 NN denotes tetracycline
T23697 223-224 HYPH denotes -
T23696 224-234 VBN denotes controlled
T23694 235-249 NN denotes transactivator
T23698 250-251 -LRB- denotes (
T23699 251-254 NN denotes tTA
T23700 254-255 -RRB- denotes )
T23701 256-260 IN denotes from
T23702 261-264 DT denotes the
T23704 265-270 NN denotes liver
T23705 271-279 JJ denotes specific
T23706 280-283 NN denotes LAP
T23703 284-292 NN denotes promoter
T23707 292-293 . denotes .
T23708 293-522 sentence denotes The TRE-E2 transgene contains the tetracycline response element (TRE) as part of the promoter, a synthetic intron (thin line), the human E2 cDNA, an alanine spacer, a c-myc epitope tag, and SV40 derived polyadenylation sequence.
T23709 294-297 DT denotes The
T23711 298-301 NN denotes TRE
T23713 301-302 HYPH denotes -
T23712 302-304 NN denotes E2
T23710 305-314 NN denotes transgene
T23714 315-323 VBZ denotes contains
T23715 324-327 DT denotes the
T23717 328-340 NN denotes tetracycline
T23718 341-349 NN denotes response
T23716 350-357 NN denotes element
T23719 358-359 -LRB- denotes (
T23720 359-362 NN denotes TRE
T23721 362-363 -RRB- denotes )
T23722 364-366 IN denotes as
T23723 367-371 NN denotes part
T23724 372-374 IN denotes of
T23725 375-378 DT denotes the
T23726 379-387 NN denotes promoter
T23727 387-389 , denotes ,
T23728 389-390 DT denotes a
T23730 391-400 JJ denotes synthetic
T23729 401-407 NN denotes intron
T23731 408-409 -LRB- denotes (
T23733 409-413 JJ denotes thin
T23732 414-418 NN denotes line
T23734 418-419 -RRB- denotes )
T23735 419-421 , denotes ,
T23736 421-424 DT denotes the
T23738 425-430 JJ denotes human
T23739 431-433 NN denotes E2
T23737 434-438 NN denotes cDNA
T23740 438-440 , denotes ,
T23741 440-442 DT denotes an
T23743 443-450 NN denotes alanine
T23742 451-457 NN denotes spacer
T23744 457-459 , denotes ,
T23745 459-460 DT denotes a
T23747 461-462 NN denotes c
T23749 462-463 HYPH denotes -
T23748 463-466 NN denotes myc
T23750 467-474 NN denotes epitope
T23746 475-478 NN denotes tag
T23751 478-480 , denotes ,
T23752 480-483 CC denotes and
T23753 484-488 NN denotes SV40
T23754 489-496 VBN denotes derived
T23756 497-512 NN denotes polyadenylation
T23755 513-521 NN denotes sequence
T23757 521-522 . denotes .
T23758 522-590 sentence denotes This construct was used to create several lines of transgenic mice.
T23759 523-527 DT denotes This
T23760 528-537 NN denotes construct
T23762 538-541 VBD denotes was
T23761 542-546 VBN denotes used
T23763 547-549 TO denotes to
T23764 550-556 VB denotes create
T23765 557-564 JJ denotes several
T23766 565-570 NNS denotes lines
T23767 571-573 IN denotes of
T23768 574-584 JJ denotes transgenic
T23769 585-589 NNS denotes mice
T23770 589-590 . denotes .
T23771 590-677 sentence denotes B, Western blot analysis of E2 protein in liver of control and intermediate MSUD mice.
T23772 591-592 LS denotes B
T23774 592-594 , denotes ,
T23775 594-601 NNP denotes Western
T23776 602-606 NN denotes blot
T23773 607-615 NN denotes analysis
T23777 616-618 IN denotes of
T23778 619-621 NN denotes E2
T23779 622-629 NN denotes protein
T23780 630-632 IN denotes in
T23781 633-638 NN denotes liver
T23782 639-641 IN denotes of
T23783 642-649 NN denotes control
T23785 650-653 CC denotes and
T23786 654-666 JJ denotes intermediate
T23787 667-671 NN denotes MSUD
T23784 672-676 NNS denotes mice
T23788 676-677 . denotes .
T23789 677-892 sentence denotes Note that the amount of human E2 protein (predicted MW ~54 Kd) in mice from Lines A and 525 A was variable but in many of the animals the amount was similar to the amount of mouse E2 (MW=~47 Kd) in control animals.
T23790 678-682 VB denotes Note
T23791 683-687 IN denotes that
T23793 688-691 DT denotes the
T23794 692-698 NN denotes amount
T23795 699-701 IN denotes of
T23796 702-707 JJ denotes human
T23798 708-710 NN denotes E2
T23797 711-718 NN denotes protein
T23799 719-720 -LRB- denotes (
T23801 720-729 VBN denotes predicted
T23800 730-732 NN denotes MW
T23802 733-734 SYM denotes ~
T23803 734-736 CD denotes 54
T23804 737-739 NN denotes Kd
T23805 739-740 -RRB- denotes )
T23806 741-743 IN denotes in
T23807 744-748 NNS denotes mice
T23808 749-753 IN denotes from
T23809 754-759 NNS denotes Lines
T23810 760-761 NN denotes A
T23811 762-765 CC denotes and
T23812 766-769 CD denotes 525
T23813 770-771 NN denotes A
T23792 772-775 VBD denotes was
T23814 776-784 JJ denotes variable
T23815 785-788 CC denotes but
T23816 789-791 IN denotes in
T23818 792-796 JJ denotes many
T23819 797-799 IN denotes of
T23820 800-803 DT denotes the
T23821 804-811 NNS denotes animals
T23822 812-815 DT denotes the
T23823 816-822 NN denotes amount
T23817 823-826 VBD denotes was
T23824 827-834 JJ denotes similar
T23825 835-837 IN denotes to
T23826 838-841 DT denotes the
T23827 842-848 NN denotes amount
T23828 849-851 IN denotes of
T23829 852-857 NN denotes mouse
T23830 858-860 NN denotes E2
T23831 861-862 -LRB- denotes (
T23833 862-864 NN denotes MW
T23834 864-865 SYM denotes =
T23835 865-866 SYM denotes ~
T23836 866-868 CD denotes 47
T23832 869-871 NN denotes Kd
T23837 871-872 -RRB- denotes )
T23838 873-875 IN denotes in
T23839 876-883 NN denotes control
T23840 884-891 NNS denotes animals
T23841 891-892 . denotes .
T23842 892-1041 sentence denotes Re-probing with a c-myc tag antibody confirmed the presence of the transgene derived, c-myc tagged, human E2 in transgenic mice but not in controls.
T23843 893-895 NN denotes Re
T23845 895-896 HYPH denotes -
T23844 896-903 NN denotes probing
T23847 904-908 IN denotes with
T23848 909-910 DT denotes a
T23850 911-912 NN denotes c
T23852 912-913 HYPH denotes -
T23851 913-916 NN denotes myc
T23853 917-920 NN denotes tag
T23849 921-929 NN denotes antibody
T23846 930-939 VBD denotes confirmed
T23854 940-943 DT denotes the
T23855 944-952 NN denotes presence
T23856 953-955 IN denotes of
T23857 956-959 DT denotes the
T23859 960-969 NN denotes transgene
T23860 970-977 VBN denotes derived
T23861 977-979 , denotes ,
T23862 979-980 NN denotes c
T23864 980-981 HYPH denotes -
T23863 981-984 NN denotes myc
T23865 985-991 VBN denotes tagged
T23866 991-993 , denotes ,
T23867 993-998 JJ denotes human
T23858 999-1001 NN denotes E2
T23868 1002-1004 IN denotes in
T23869 1005-1015 JJ denotes transgenic
T23870 1016-1020 NNS denotes mice
T23871 1021-1024 CC denotes but
T23872 1025-1028 RB denotes not
T23873 1029-1031 IN denotes in
T23874 1032-1040 NNS denotes controls
T23875 1040-1041 . denotes .
T23876 1041-1174 sentence denotes Western blot analysis of brain (C), kidney (D), and muscle (E) revealed negligible amounts of transgene derived E2 in those tissues.
T23877 1042-1049 NNP denotes Western
T23878 1050-1054 NN denotes blot
T23879 1055-1063 NN denotes analysis
T23881 1064-1066 IN denotes of
T23882 1067-1072 NN denotes brain
T23883 1073-1074 -LRB- denotes (
T23884 1074-1075 NN denotes C
T23885 1075-1076 -RRB- denotes )
T23886 1076-1078 , denotes ,
T23887 1078-1084 NN denotes kidney
T23888 1085-1086 -LRB- denotes (
T23889 1086-1087 NN denotes D
T23890 1087-1088 -RRB- denotes )
T23891 1088-1090 , denotes ,
T23892 1090-1093 CC denotes and
T23893 1094-1100 NN denotes muscle
T23894 1101-1102 -LRB- denotes (
T23895 1102-1103 NN denotes E
T23896 1103-1104 -RRB- denotes )
T23880 1105-1113 VBD denotes revealed
T23897 1114-1124 JJ denotes negligible
T23898 1125-1132 NNS denotes amounts
T23899 1133-1135 IN denotes of
T23900 1136-1145 NN denotes transgene
T23901 1146-1153 VBN denotes derived
T23902 1154-1156 NN denotes E2
T23903 1157-1159 IN denotes in
T23904 1160-1165 DT denotes those
T23905 1166-1173 NNS denotes tissues
T23906 1173-1174 . denotes .
T23907 1174-1285 sentence denotes All blots were re-probed with an actin antibody to allow amount of protein loaded in each lane to be compared.
T23908 1175-1178 DT denotes All
T23909 1179-1184 NNS denotes blots
T23911 1185-1189 VBD denotes were
T23910 1190-1199 VBN denotes re-probed
T23912 1200-1204 IN denotes with
T23913 1205-1207 DT denotes an
T23915 1208-1213 NN denotes actin
T23914 1214-1222 NN denotes antibody
T23916 1223-1225 TO denotes to
T23917 1226-1231 VB denotes allow
T23918 1232-1238 NN denotes amount
T23920 1239-1241 IN denotes of
T23921 1242-1249 NN denotes protein
T23922 1250-1256 VBN denotes loaded
T23923 1257-1259 IN denotes in
T23924 1260-1264 DT denotes each
T23925 1265-1269 NN denotes lane
T23926 1270-1272 TO denotes to
T23927 1273-1275 VB denotes be
T23919 1276-1284 VBN denotes compared
T23928 1284-1285 . denotes .
R6739 T23655 T23656 amod Transgenic,production
R6740 T23657 T23656 compound mouse,production
R6741 T23658 T23656 cc and,production
R6742 T23659 T23656 conj characterization,production
R6743 T23660 T23656 punct .,production
R6744 T23662 T23663 meta A,strategy
R6745 T23664 T23663 punct ", ",strategy
R6746 T23665 T23663 amod Transgenic,strategy
R6747 T23666 T23663 acl used,strategy
R6748 T23667 T23668 aux to,produce
R6749 T23668 T23666 advcl produce,used
R6750 T23669 T23668 dobj mice,produce
R6751 T23670 T23671 dep that,express
R6752 T23671 T23669 relcl express,mice
R6753 T23672 T23673 amod human,E2
R6754 T23673 T23671 dobj E2,express
R6755 T23674 T23663 punct .,strategy
R6756 T23676 T23677 nmod LAP,tTA
R6757 T23677 T23679 nmod tTA,mice
R6758 T23678 T23677 punct -,tTA
R6759 T23679 T23681 nsubjpass mice,described
R6760 T23680 T23679 amod transgenic,mice
R6761 T23682 T23681 aux have,described
R6762 T23683 T23681 auxpass been,described
R6763 T23684 T23681 advmod previously,described
R6764 T23685 T23686 punct [,34
R6765 T23686 T23681 parataxis 34,described
R6766 T23687 T23686 punct ],34
R6767 T23688 T23681 punct .,described
R6768 T23690 T23691 det These,mice
R6769 T23691 T23692 nsubj mice,express
R6770 T23693 T23694 det the,transactivator
R6771 T23694 T23692 dobj transactivator,express
R6772 T23695 T23696 npadvmod tetracycline,controlled
R6773 T23696 T23694 amod controlled,transactivator
R6774 T23697 T23696 punct -,controlled
R6775 T23698 T23694 punct (,transactivator
R6776 T23699 T23694 appos tTA,transactivator
R6777 T23700 T23694 punct ),transactivator
R6778 T23701 T23694 prep from,transactivator
R6779 T23702 T23703 det the,promoter
R6780 T23703 T23701 pobj promoter,from
R6781 T23704 T23705 npadvmod liver,specific
R6782 T23705 T23703 amod specific,promoter
R6783 T23706 T23703 compound LAP,promoter
R6784 T23707 T23692 punct .,express
R6785 T23709 T23710 det The,transgene
R6786 T23710 T23714 nsubj transgene,contains
R6787 T23711 T23712 compound TRE,E2
R6788 T23712 T23710 compound E2,transgene
R6789 T23713 T23712 punct -,E2
R6790 T23715 T23716 det the,element
R6791 T23716 T23714 dobj element,contains
R6792 T23717 T23716 compound tetracycline,element
R6793 T23718 T23716 compound response,element
R6794 T23719 T23716 punct (,element
R6795 T23720 T23716 appos TRE,element
R6796 T23721 T23716 punct ),element
R6797 T23722 T23716 prep as,element
R6798 T23723 T23722 pobj part,as
R6799 T23724 T23723 prep of,part
R6800 T23725 T23726 det the,promoter
R6801 T23726 T23724 pobj promoter,of
R6802 T23727 T23716 punct ", ",element
R6803 T23728 T23729 det a,intron
R6804 T23729 T23716 conj intron,element
R6805 T23730 T23729 amod synthetic,intron
R6806 T23731 T23732 punct (,line
R6807 T23732 T23729 parataxis line,intron
R6808 T23733 T23732 amod thin,line
R6809 T23734 T23732 punct ),line
R6810 T23735 T23729 punct ", ",intron
R6811 T23736 T23737 det the,cDNA
R6812 T23737 T23729 conj cDNA,intron
R6813 T23738 T23737 amod human,cDNA
R6814 T23739 T23737 compound E2,cDNA
R6815 T23740 T23737 punct ", ",cDNA
R6816 T23741 T23742 det an,spacer
R6817 T23742 T23737 conj spacer,cDNA
R6818 T23743 T23742 compound alanine,spacer
R6819 T23744 T23742 punct ", ",spacer
R6820 T23745 T23746 det a,tag
R6821 T23746 T23742 conj tag,spacer
R6822 T23747 T23748 compound c,myc
R6823 T23748 T23746 compound myc,tag
R6824 T23749 T23748 punct -,myc
R6825 T23750 T23746 compound epitope,tag
R6826 T23751 T23746 punct ", ",tag
R6827 T23752 T23746 cc and,tag
R6828 T23753 T23754 npadvmod SV40,derived
R6829 T23754 T23755 amod derived,sequence
R6830 T23755 T23746 conj sequence,tag
R6831 T23756 T23755 compound polyadenylation,sequence
R6832 T23757 T23714 punct .,contains
R6833 T23759 T23760 det This,construct
R6834 T23760 T23761 nsubjpass construct,used
R6835 T23762 T23761 auxpass was,used
R6836 T23763 T23764 aux to,create
R6837 T23764 T23761 advcl create,used
R6838 T23765 T23766 amod several,lines
R6839 T23766 T23764 dobj lines,create
R6840 T23767 T23766 prep of,lines
R6841 T23768 T23769 amod transgenic,mice
R6842 T23769 T23767 pobj mice,of
R6843 T23770 T23761 punct .,used
R6844 T23772 T23773 meta B,analysis
R6845 T23774 T23773 punct ", ",analysis
R6846 T23775 T23776 compound Western,blot
R6847 T23776 T23773 compound blot,analysis
R6848 T23777 T23773 prep of,analysis
R6849 T23778 T23779 compound E2,protein
R6850 T23779 T23777 pobj protein,of
R6851 T23780 T23779 prep in,protein
R6852 T23781 T23780 pobj liver,in
R6853 T23782 T23781 prep of,liver
R6854 T23783 T23784 nmod control,mice
R6855 T23784 T23782 pobj mice,of
R6856 T23785 T23783 cc and,control
R6857 T23786 T23783 conj intermediate,control
R6858 T23787 T23784 compound MSUD,mice
R6859 T23788 T23773 punct .,analysis
R6860 T23791 T23792 mark that,was
R6861 T23792 T23790 ccomp was,Note
R6862 T23793 T23794 det the,amount
R6863 T23794 T23792 nsubj amount,was
R6864 T23795 T23794 prep of,amount
R6865 T23796 T23797 amod human,protein
R6866 T23797 T23795 pobj protein,of
R6867 T23798 T23797 compound E2,protein
R6868 T23799 T23800 punct (,MW
R6869 T23800 T23797 parataxis MW,protein
R6870 T23801 T23800 amod predicted,MW
R6871 T23802 T23803 punct ~,54
R6872 T23803 T23804 nummod 54,Kd
R6873 T23804 T23800 appos Kd,MW
R6874 T23805 T23800 punct ),MW
R6875 T23806 T23794 prep in,amount
R6876 T23807 T23806 pobj mice,in
R6877 T23808 T23807 prep from,mice
R6878 T23809 T23810 compound Lines,A
R6879 T23810 T23808 pobj A,from
R6880 T23811 T23810 cc and,A
R6881 T23812 T23813 nummod 525,A
R6882 T23813 T23810 conj A,A
R6883 T23814 T23792 acomp variable,was
R6884 T23815 T23792 cc but,was
R6885 T23816 T23817 prep in,was
R6886 T23817 T23792 conj was,was
R6887 T23818 T23816 pobj many,in
R6888 T23819 T23818 prep of,many
R6889 T23820 T23821 det the,animals
R6890 T23821 T23819 pobj animals,of
R6891 T23822 T23823 det the,amount
R6892 T23823 T23817 nsubj amount,was
R6893 T23824 T23817 acomp similar,was
R6894 T23825 T23824 prep to,similar
R6895 T23826 T23827 det the,amount
R6896 T23827 T23825 pobj amount,to
R6897 T23828 T23827 prep of,amount
R6898 T23829 T23830 compound mouse,E2
R6899 T23830 T23828 pobj E2,of
R6900 T23831 T23832 punct (,Kd
R6901 T23832 T23830 parataxis Kd,E2
R6902 T23833 T23832 nsubj MW,Kd
R6903 T23834 T23832 punct =,Kd
R6904 T23835 T23836 punct ~,47
R6905 T23836 T23832 nummod 47,Kd
R6906 T23837 T23832 punct ),Kd
R6907 T23838 T23827 prep in,amount
R6908 T23839 T23840 compound control,animals
R6909 T23840 T23838 pobj animals,in
R6910 T23841 T23790 punct .,Note
R6911 T23843 T23844 compound Re,probing
R6912 T23844 T23846 nsubj probing,confirmed
R6913 T23845 T23844 punct -,probing
R6914 T23847 T23844 prep with,probing
R6915 T23848 T23849 det a,antibody
R6916 T23849 T23847 pobj antibody,with
R6917 T23850 T23851 compound c,myc
R6918 T23851 T23849 compound myc,antibody
R6919 T23852 T23851 punct -,myc
R6920 T23853 T23849 compound tag,antibody
R6921 T23854 T23855 det the,presence
R6922 T23855 T23846 dobj presence,confirmed
R6923 T23856 T23855 prep of,presence
R6924 T23857 T23858 det the,E2
R6925 T23858 T23856 pobj E2,of
R6926 T23859 T23860 npadvmod transgene,derived
R6927 T23860 T23858 amod derived,E2
R6928 T23861 T23858 punct ", ",E2
R6929 T23862 T23863 compound c,myc
R6930 T23863 T23865 npadvmod myc,tagged
R6931 T23864 T23863 punct -,myc
R6932 T23865 T23858 amod tagged,E2
R6933 T23866 T23858 punct ", ",E2
R6934 T23867 T23858 amod human,E2
R6935 T23868 T23846 prep in,confirmed
R6936 T23869 T23870 amod transgenic,mice
R6937 T23870 T23868 pobj mice,in
R6938 T23871 T23868 cc but,in
R6939 T23872 T23871 neg not,but
R6940 T23873 T23868 conj in,in
R6941 T23874 T23873 pobj controls,in
R6942 T23875 T23846 punct .,confirmed
R6943 T23877 T23878 compound Western,blot
R6944 T23878 T23879 compound blot,analysis
R6945 T23879 T23880 nsubj analysis,revealed
R6946 T23881 T23879 prep of,analysis
R6947 T23882 T23881 pobj brain,of
R6948 T23883 T23884 punct (,C
R6949 T23884 T23882 parataxis C,brain
R6950 T23885 T23884 punct ),C
R6951 T23886 T23882 punct ", ",brain
R6952 T23887 T23882 conj kidney,brain
R6953 T23888 T23889 punct (,D
R6954 T23889 T23887 parataxis D,kidney
R6955 T23890 T23889 punct ),D
R6956 T23891 T23887 punct ", ",kidney
R6957 T23892 T23887 cc and,kidney
R6958 T23893 T23887 conj muscle,kidney
R6959 T23894 T23895 punct (,E
R6960 T23895 T23893 parataxis E,muscle
R6961 T23896 T23895 punct ),E
R6962 T23897 T23898 amod negligible,amounts
R6963 T23898 T23880 dobj amounts,revealed
R6964 T23899 T23898 prep of,amounts
R6965 T23900 T23901 npadvmod transgene,derived
R6966 T23901 T23902 amod derived,E2
R6967 T23902 T23899 pobj E2,of
R6968 T23903 T23880 prep in,revealed
R6969 T23904 T23905 det those,tissues
R6970 T23905 T23903 pobj tissues,in
R6971 T23906 T23880 punct .,revealed
R6972 T23908 T23909 det All,blots
R6973 T23909 T23910 nsubjpass blots,re-probed
R6974 T23911 T23910 auxpass were,re-probed
R6975 T23912 T23910 prep with,re-probed
R6976 T23913 T23914 det an,antibody
R6977 T23914 T23912 pobj antibody,with
R6978 T23915 T23914 compound actin,antibody
R6979 T23916 T23917 aux to,allow
R6980 T23917 T23910 advcl allow,re-probed
R6981 T23918 T23919 nsubjpass amount,compared
R6982 T23919 T23917 ccomp compared,allow
R6983 T23920 T23918 prep of,amount
R6984 T23921 T23920 pobj protein,of
R6985 T23922 T23921 acl loaded,protein
R6986 T23923 T23922 prep in,loaded
R6987 T23924 T23925 det each,lane
R6988 T23925 T23923 pobj lane,in
R6989 T23926 T23919 aux to,compared
R6990 T23927 T23919 auxpass be,compared
R6991 T23928 T23910 punct .,re-probed