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PMC:1448208 / 16344-18850 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T10367 0-10 NN denotes Production
T10368 11-13 IN denotes of
T10369 14-16 NN denotes E2
T10371 17-21 NN denotes gene
T10372 22-30 NN denotes knockout
T10370 31-35 NNS denotes mice
T10373 35-128 sentence denotes To create E2 knockout mice, we used gene targeting in mouse ES cells to disrupt the E2 gene.
T10374 36-38 TO denotes To
T10375 39-45 VB denotes create
T10377 46-48 NN denotes E2
T10379 49-57 NN denotes knockout
T10378 58-62 NNS denotes mice
T10380 62-64 , denotes ,
T10381 64-66 PRP denotes we
T10376 67-71 VBD denotes used
T10382 72-76 NN denotes gene
T10383 77-86 NN denotes targeting
T10384 87-89 IN denotes in
T10385 90-95 NN denotes mouse
T10387 96-98 NN denotes ES
T10386 99-104 NNS denotes cells
T10388 105-107 TO denotes to
T10389 108-115 VB denotes disrupt
T10390 116-119 DT denotes the
T10392 120-122 NN denotes E2
T10391 123-127 NN denotes gene
T10393 127-128 . denotes .
T10394 128-203 sentence denotes The overall strategy for disrupting the E2 gene is illustrated in Fig. 1A.
T10395 129-132 DT denotes The
T10397 133-140 JJ denotes overall
T10396 141-149 NN denotes strategy
T10399 150-153 IN denotes for
T10400 154-164 VBG denotes disrupting
T10401 165-168 DT denotes the
T10403 169-171 NN denotes E2
T10402 172-176 NN denotes gene
T10404 177-179 VBZ denotes is
T10398 180-191 VBN denotes illustrated
T10405 192-194 IN denotes in
T10406 195-199 NN denotes Fig.
T10407 200-202 NN denotes 1A
T10408 202-203 . denotes .
T10409 203-388 sentence denotes The gene targeting construct was designed to replace a 1.67 kb EcoRV/Smal genomic DNA fragment encompassing part of Exon 4 and all of Exon 5 with the PGKneo selectable marker cassette.
T10410 204-207 DT denotes The
T10412 208-212 NN denotes gene
T10413 213-222 NN denotes targeting
T10411 223-232 NN denotes construct
T10415 233-236 VBD denotes was
T10414 237-245 VBN denotes designed
T10416 246-248 TO denotes to
T10417 249-256 VB denotes replace
T10418 257-258 DT denotes a
T10420 259-263 CD denotes 1.67
T10421 264-266 NN denotes kb
T10422 267-272 NN denotes EcoRV
T10424 272-273 HYPH denotes /
T10423 273-277 NN denotes Smal
T10425 278-285 JJ denotes genomic
T10426 286-289 NN denotes DNA
T10419 290-298 NN denotes fragment
T10427 299-311 VBG denotes encompassing
T10428 312-316 NN denotes part
T10429 317-319 IN denotes of
T10430 320-324 NN denotes Exon
T10431 325-326 CD denotes 4
T10432 327-330 CC denotes and
T10433 331-334 DT denotes all
T10434 335-337 IN denotes of
T10435 338-342 NN denotes Exon
T10436 343-344 CD denotes 5
T10437 345-349 IN denotes with
T10438 350-353 DT denotes the
T10440 354-360 NN denotes PGKneo
T10441 361-371 JJ denotes selectable
T10442 372-378 NN denotes marker
T10439 379-387 NN denotes cassette
T10443 387-388 . denotes .
T10444 388-580 sentence denotes Of 522 ES cell clones screened for targeting by Southern blot analysis, 29 (5%) displayed the predicted restriction fragment length polymorphisms indicative of gene targeting at the E2 locus.
T10445 389-391 IN denotes Of
T10447 392-395 CD denotes 522
T10449 396-398 NN denotes ES
T10450 399-403 NN denotes cell
T10448 404-410 NNS denotes clones
T10451 411-419 VBN denotes screened
T10452 420-423 IN denotes for
T10453 424-433 NN denotes targeting
T10454 434-436 IN denotes by
T10455 437-445 NNP denotes Southern
T10456 446-450 NN denotes blot
T10457 451-459 NN denotes analysis
T10458 459-461 , denotes ,
T10459 461-463 CD denotes 29
T10460 464-465 -LRB- denotes (
T10462 465-466 CD denotes 5
T10461 466-467 NN denotes %
T10463 467-468 -RRB- denotes )
T10446 469-478 VBD denotes displayed
T10464 479-482 DT denotes the
T10466 483-492 VBN denotes predicted
T10467 493-504 NN denotes restriction
T10469 505-513 NN denotes fragment
T10468 514-520 NN denotes length
T10465 521-534 NNS denotes polymorphisms
T10470 535-545 JJ denotes indicative
T10471 546-548 IN denotes of
T10472 549-553 NN denotes gene
T10473 554-563 VBG denotes targeting
T10474 564-566 IN denotes at
T10475 567-570 DT denotes the
T10477 571-573 NN denotes E2
T10476 574-579 NN denotes locus
T10478 579-580 . denotes .
T10479 580-802 sentence denotes As illustrated in Fig. 1A and 1B, an E2 Exon 6 specific probe, which is external to the targeting construct, hybridizes to only a ~16 kb BglI restriction fragment from the wild type allele in the parental R1 ES cell line.
T10480 581-583 IN denotes As
T10481 584-595 VBN denotes illustrated
T10483 596-598 IN denotes in
T10484 599-603 NN denotes Fig.
T10485 604-606 NN denotes 1A
T10486 607-610 CC denotes and
T10487 611-613 NN denotes 1B
T10488 613-615 , denotes ,
T10489 615-617 DT denotes an
T10491 618-620 NN denotes E2
T10492 621-625 NN denotes Exon
T10493 626-627 CD denotes 6
T10494 628-636 JJ denotes specific
T10490 637-642 NN denotes probe
T10495 642-644 , denotes ,
T10496 644-649 WDT denotes which
T10497 650-652 VBZ denotes is
T10498 653-661 JJ denotes external
T10499 662-664 IN denotes to
T10500 665-668 DT denotes the
T10502 669-678 NN denotes targeting
T10501 679-688 NN denotes construct
T10503 688-690 , denotes ,
T10482 690-700 VBZ denotes hybridizes
T10504 701-703 IN denotes to
T10505 704-708 RB denotes only
T10507 709-710 DT denotes a
T10508 711-712 SYM denotes ~
T10509 712-714 CD denotes 16
T10510 715-717 NN denotes kb
T10511 718-722 NN denotes BglI
T10512 723-734 NN denotes restriction
T10506 735-743 NN denotes fragment
T10513 744-748 IN denotes from
T10514 749-752 DT denotes the
T10516 753-757 JJ denotes wild
T10517 758-762 NN denotes type
T10515 763-769 NN denotes allele
T10518 770-772 IN denotes in
T10519 773-776 DT denotes the
T10521 777-785 JJ denotes parental
T10522 786-788 NN denotes R1
T10523 789-791 NN denotes ES
T10524 792-796 NN denotes cell
T10520 797-801 NN denotes line
T10525 801-802 . denotes .
T10526 802-900 sentence denotes In correctly targeted ES cells, this probe also hybridizes to a ~11 kb BglI restriction fragment.
T10527 803-805 IN denotes In
T10529 806-815 RB denotes correctly
T10530 816-824 VBN denotes targeted
T10532 825-827 NN denotes ES
T10531 828-833 NNS denotes cells
T10533 833-835 , denotes ,
T10534 835-839 DT denotes this
T10535 840-845 NN denotes probe
T10536 846-850 RB denotes also
T10528 851-861 VBZ denotes hybridizes
T10537 862-864 IN denotes to
T10538 865-866 DT denotes a
T10540 867-868 SYM denotes ~
T10541 868-870 CD denotes 11
T10542 871-873 NN denotes kb
T10543 874-878 NN denotes BglI
T10544 879-890 NN denotes restriction
T10539 891-899 NN denotes fragment
T10545 899-900 . denotes .
T10546 900-997 sentence denotes Targeting was confirmed with several additional restriction enzymes and probes (data not shown).
T10547 901-910 NN denotes Targeting
T10549 911-914 VBD denotes was
T10548 915-924 VBN denotes confirmed
T10550 925-929 IN denotes with
T10551 930-937 JJ denotes several
T10553 938-948 JJ denotes additional
T10554 949-960 NN denotes restriction
T10552 961-968 NNS denotes enzymes
T10555 969-972 CC denotes and
T10556 973-979 NNS denotes probes
T10557 980-981 -LRB- denotes (
T10559 981-985 NNS denotes data
T10560 986-989 RB denotes not
T10558 990-995 VBN denotes shown
T10561 995-996 -RRB- denotes )
T10562 996-997 . denotes .
T10563 997-1087 sentence denotes Correctly targeted ES cells were microinjected into blastocysts to produce chimeric mice.
T10564 998-1007 RB denotes Correctly
T10565 1008-1016 VBN denotes targeted
T10567 1017-1019 NN denotes ES
T10566 1020-1025 NNS denotes cells
T10569 1026-1030 VBD denotes were
T10568 1031-1044 VBN denotes microinjected
T10570 1045-1049 IN denotes into
T10571 1050-1061 NNS denotes blastocysts
T10572 1062-1064 TO denotes to
T10573 1065-1072 VB denotes produce
T10574 1073-1081 JJ denotes chimeric
T10575 1082-1086 NNS denotes mice
T10576 1086-1087 . denotes .
T10577 1087-1125 sentence denotes Chimerics were bred to C57BL/6J mice.
T10578 1088-1097 NNS denotes Chimerics
T10580 1098-1102 VBD denotes were
T10579 1103-1107 VBN denotes bred
T10581 1108-1110 IN denotes to
T10582 1111-1116 NN denotes C57BL
T10584 1116-1117 HYPH denotes /
T10583 1117-1119 NN denotes 6J
T10585 1120-1124 NNS denotes mice
T10586 1124-1125 . denotes .
T10587 1125-1291 sentence denotes Following germline transmission of the targeted allele, heterozygous mice were interbred to produce wild type (+/+), heterozygous (+/-) and homozygous (-/-) animals.
T10588 1126-1135 VBG denotes Following
T10590 1136-1144 NN denotes germline
T10591 1145-1157 NN denotes transmission
T10592 1158-1160 IN denotes of
T10593 1161-1164 DT denotes the
T10595 1165-1173 VBN denotes targeted
T10594 1174-1180 NN denotes allele
T10596 1180-1182 , denotes ,
T10597 1182-1194 JJ denotes heterozygous
T10598 1195-1199 NNS denotes mice
T10599 1200-1204 VBD denotes were
T10589 1205-1214 VBN denotes interbred
T10600 1215-1217 TO denotes to
T10601 1218-1225 VB denotes produce
T10602 1226-1230 JJ denotes wild
T10603 1231-1235 NN denotes type
T10605 1236-1237 -LRB- denotes (
T10607 1237-1238 SYM denotes +
T10608 1238-1239 HYPH denotes /
T10606 1239-1240 SYM denotes +
T10609 1240-1241 -RRB- denotes )
T10610 1241-1243 , denotes ,
T10611 1243-1255 JJ denotes heterozygous
T10612 1256-1257 -LRB- denotes (
T10614 1257-1258 SYM denotes +
T10615 1258-1259 HYPH denotes /
T10613 1259-1260 SYM denotes -
T10616 1260-1261 -RRB- denotes )
T10617 1262-1265 CC denotes and
T10618 1266-1276 JJ denotes homozygous
T10619 1277-1278 -LRB- denotes (
T10621 1278-1279 SYM denotes -
T10622 1279-1280 HYPH denotes /
T10620 1280-1281 SYM denotes -
T10623 1281-1282 -RRB- denotes )
T10604 1283-1290 NNS denotes animals
T10624 1290-1291 . denotes .
T10625 1291-1338 sentence denotes Mice were genotyped by Southern blot analysis.
T10626 1292-1296 NNS denotes Mice
T10628 1297-1301 VBD denotes were
T10627 1302-1311 VBN denotes genotyped
T10629 1312-1314 IN denotes by
T10630 1315-1323 NNP denotes Southern
T10631 1324-1328 NN denotes blot
T10632 1329-1337 NN denotes analysis
T10633 1337-1338 . denotes .
T10634 1338-1521 sentence denotes The Exon 6 specific probe hybridized to only a ~16 kb BglI restriction fragment in +/+ mice, a ~11 kb BglI fragment in -/- mice, and to both of these fragments in +/- mice (Fig. 1B).
T10635 1339-1342 DT denotes The
T10637 1343-1347 NN denotes Exon
T10639 1348-1349 CD denotes 6
T10638 1350-1358 JJ denotes specific
T10636 1359-1364 NN denotes probe
T10640 1365-1375 VBN denotes hybridized
T10641 1376-1378 IN denotes to
T10642 1379-1383 RB denotes only
T10644 1384-1385 DT denotes a
T10645 1386-1387 SYM denotes ~
T10646 1387-1389 CD denotes 16
T10647 1390-1392 NN denotes kb
T10648 1393-1397 NN denotes BglI
T10649 1398-1409 NN denotes restriction
T10643 1410-1418 NN denotes fragment
T10650 1419-1421 IN denotes in
T10651 1422-1423 SYM denotes +
T10653 1423-1424 HYPH denotes /
T10652 1424-1425 SYM denotes +
T10654 1426-1430 NNS denotes mice
T10655 1430-1432 , denotes ,
T10656 1432-1433 DT denotes a
T10658 1434-1435 SYM denotes ~
T10659 1435-1437 CD denotes 11
T10660 1438-1440 NN denotes kb
T10661 1441-1445 NN denotes BglI
T10657 1446-1454 NN denotes fragment
T10662 1455-1457 IN denotes in
T10663 1458-1459 SYM denotes -
T10665 1459-1460 HYPH denotes /
T10664 1460-1461 SYM denotes -
T10666 1462-1466 NNS denotes mice
T10667 1466-1468 , denotes ,
T10668 1468-1471 CC denotes and
T10669 1472-1474 IN denotes to
T10670 1475-1479 DT denotes both
T10671 1480-1482 IN denotes of
T10672 1483-1488 DT denotes these
T10673 1489-1498 NNS denotes fragments
T10674 1499-1501 IN denotes in
T10675 1502-1503 SYM denotes +
T10677 1503-1504 HYPH denotes /
T10676 1504-1505 SYM denotes -
T10678 1506-1510 NNS denotes mice
T10679 1511-1512 -LRB- denotes (
T10681 1512-1516 NN denotes Fig.
T10680 1517-1519 NN denotes 1B
T10682 1519-1520 -RRB- denotes )
T10683 1520-1521 . denotes .
T10684 1521-1594 sentence denotes Mice homozygous for the E2 mutation were born at the expected frequency.
T10685 1522-1526 NNS denotes Mice
T10687 1527-1537 JJ denotes homozygous
T10688 1538-1541 IN denotes for
T10689 1542-1545 DT denotes the
T10691 1546-1548 NN denotes E2
T10690 1549-1557 NN denotes mutation
T10692 1558-1562 VBD denotes were
T10686 1563-1567 VBN denotes born
T10693 1568-1570 IN denotes at
T10694 1571-1574 DT denotes the
T10696 1575-1583 VBN denotes expected
T10695 1584-1593 NN denotes frequency
T10697 1593-1594 . denotes .
T10698 1594-1742 sentence denotes Genotype analysis of pups derived from +/- by +/- matings revealed that +/+, +/-, and -/- mice were present at nearly the expected 1:2:1 frequency.
T10699 1595-1603 NN denotes Genotype
T10700 1604-1612 NN denotes analysis
T10702 1613-1615 IN denotes of
T10703 1616-1620 NNS denotes pups
T10704 1621-1628 VBN denotes derived
T10705 1629-1633 IN denotes from
T10706 1634-1635 SYM denotes +
T10708 1635-1636 HYPH denotes /
T10707 1636-1637 SYM denotes -
T10710 1638-1640 IN denotes by
T10711 1641-1642 SYM denotes +
T10713 1642-1643 HYPH denotes /
T10712 1643-1644 SYM denotes -
T10709 1645-1652 NNS denotes matings
T10701 1653-1661 VBD denotes revealed
T10714 1662-1666 IN denotes that
T10716 1667-1668 SYM denotes +
T10718 1668-1669 HYPH denotes /
T10717 1669-1670 SYM denotes +
T10720 1670-1672 , denotes ,
T10721 1672-1673 SYM denotes +
T10723 1673-1674 HYPH denotes /
T10722 1674-1675 SYM denotes -
T10724 1675-1677 , denotes ,
T10725 1677-1680 CC denotes and
T10726 1681-1682 SYM denotes -
T10728 1682-1683 HYPH denotes /
T10727 1683-1684 SYM denotes -
T10719 1685-1689 NNS denotes mice
T10715 1690-1694 VBD denotes were
T10729 1695-1702 JJ denotes present
T10730 1703-1705 IN denotes at
T10731 1706-1712 RB denotes nearly
T10733 1713-1716 DT denotes the
T10734 1717-1725 VBN denotes expected
T10735 1726-1727 CD denotes 1
T10737 1727-1728 SYM denotes :
T10738 1728-1729 CD denotes 2
T10739 1729-1730 SYM denotes :
T10736 1730-1731 CD denotes 1
T10732 1732-1741 NN denotes frequency
T10740 1741-1742 . denotes .
T10741 1742-1840 sentence denotes Of the initial 60 animals genotyped, 19 (32%) were +/+, 27 (45%) were +/-, and 14 (23%) were -/-.
T10742 1743-1745 IN denotes Of
T10744 1746-1749 DT denotes the
T10746 1750-1757 JJ denotes initial
T10747 1758-1760 CD denotes 60
T10745 1761-1768 NNS denotes animals
T10748 1769-1778 VBN denotes genotyped
T10749 1778-1780 , denotes ,
T10750 1780-1782 CD denotes 19
T10751 1783-1784 -LRB- denotes (
T10753 1784-1786 CD denotes 32
T10752 1786-1787 NN denotes %
T10754 1787-1788 -RRB- denotes )
T10743 1789-1793 VBD denotes were
T10755 1794-1795 SYM denotes +
T10757 1795-1796 HYPH denotes /
T10756 1796-1797 SYM denotes +
T10758 1797-1799 , denotes ,
T10759 1799-1801 CD denotes 27
T10761 1802-1803 -LRB- denotes (
T10763 1803-1805 CD denotes 45
T10762 1805-1806 NN denotes %
T10764 1806-1807 -RRB- denotes )
T10760 1808-1812 VBD denotes were
T10765 1813-1814 SYM denotes +
T10767 1814-1815 HYPH denotes /
T10766 1815-1816 SYM denotes -
T10768 1816-1818 , denotes ,
T10769 1818-1821 CC denotes and
T10770 1822-1824 CD denotes 14
T10772 1825-1826 -LRB- denotes (
T10774 1826-1828 CD denotes 23
T10773 1828-1829 NN denotes %
T10775 1829-1830 -RRB- denotes )
T10771 1831-1835 VBD denotes were
T10776 1836-1837 SYM denotes -
T10778 1837-1838 HYPH denotes /
T10777 1838-1839 SYM denotes -
T10779 1839-1840 . denotes .
T10780 1840-1908 sentence denotes Thus, the E2 gene was dispensable for normal embryonic development.
T10781 1841-1845 RB denotes Thus
T10783 1845-1847 , denotes ,
T10784 1847-1850 DT denotes the
T10786 1851-1853 NN denotes E2
T10785 1854-1858 NN denotes gene
T10782 1859-1862 VBD denotes was
T10787 1863-1874 JJ denotes dispensable
T10788 1875-1878 IN denotes for
T10789 1879-1885 JJ denotes normal
T10791 1886-1895 JJ denotes embryonic
T10790 1896-1907 NN denotes development
T10792 1907-1908 . denotes .
T10793 1908-1987 sentence denotes However, as expected, nearly all homozygous mice died in the perinatal period.
T10794 1909-1916 RB denotes However
T10796 1916-1918 , denotes ,
T10797 1918-1920 IN denotes as
T10798 1921-1929 VBN denotes expected
T10799 1929-1931 , denotes ,
T10800 1931-1937 RB denotes nearly
T10802 1938-1941 DT denotes all
T10803 1942-1952 JJ denotes homozygous
T10801 1953-1957 NNS denotes mice
T10795 1958-1962 VBD denotes died
T10804 1963-1965 IN denotes in
T10805 1966-1969 DT denotes the
T10807 1970-1979 JJ denotes perinatal
T10806 1980-1986 NN denotes period
T10808 1986-1987 . denotes .
T10809 1987-2138 sentence denotes Immediately following birth, homozygous pups were indistinguishable from their +/+ and +/- littermates; they were vigorous, active and able to suckle.
T10810 1988-1999 RB denotes Immediately
T10811 2000-2009 VBG denotes following
T10813 2010-2015 NN denotes birth
T10814 2015-2017 , denotes ,
T10815 2017-2027 JJ denotes homozygous
T10816 2028-2032 NNS denotes pups
T10812 2033-2037 VBD denotes were
T10818 2038-2055 JJ denotes indistinguishable
T10819 2056-2060 IN denotes from
T10820 2061-2066 PRP$ denotes their
T10822 2067-2068 SYM denotes +
T10824 2068-2069 HYPH denotes /
T10823 2069-2070 SYM denotes +
T10825 2071-2074 CC denotes and
T10826 2075-2076 SYM denotes +
T10828 2076-2077 HYPH denotes /
T10827 2077-2078 SYM denotes -
T10821 2079-2090 NNS denotes littermates
T10829 2090-2091 : denotes ;
T10830 2092-2096 PRP denotes they
T10817 2097-2101 VBD denotes were
T10831 2102-2110 JJ denotes vigorous
T10832 2110-2112 , denotes ,
T10833 2112-2118 JJ denotes active
T10834 2119-2122 CC denotes and
T10835 2123-2127 JJ denotes able
T10836 2128-2130 TO denotes to
T10837 2131-2137 VB denotes suckle
T10838 2137-2138 . denotes .
T10839 2138-2314 sentence denotes By mid to late day on postnatal day one, most -/- pups became moribund and were readily identifiable as they were lethargic, pale, and exhibited gasping respiratory movements.
T10840 2139-2141 IN denotes By
T10842 2142-2145 JJ denotes mid
T10844 2146-2148 IN denotes to
T10845 2149-2153 JJ denotes late
T10843 2154-2157 NN denotes day
T10846 2158-2160 IN denotes on
T10847 2161-2170 JJ denotes postnatal
T10848 2171-2174 NN denotes day
T10849 2175-2178 CD denotes one
T10850 2178-2180 , denotes ,
T10851 2180-2184 RBS denotes most
T10853 2185-2186 SYM denotes -
T10855 2186-2187 HYPH denotes /
T10854 2187-2188 SYM denotes -
T10852 2189-2193 NNS denotes pups
T10841 2194-2200 VBD denotes became
T10856 2201-2209 JJ denotes moribund
T10857 2210-2213 CC denotes and
T10858 2214-2218 VBD denotes were
T10859 2219-2226 RB denotes readily
T10860 2227-2239 JJ denotes identifiable
T10861 2240-2242 IN denotes as
T10863 2243-2247 PRP denotes they
T10862 2248-2252 VBD denotes were
T10864 2253-2262 JJ denotes lethargic
T10866 2262-2264 , denotes ,
T10865 2264-2268 JJ denotes pale
T10867 2268-2270 , denotes ,
T10868 2270-2273 CC denotes and
T10869 2274-2283 VBD denotes exhibited
T10870 2284-2291 VBG denotes gasping
T10872 2292-2303 JJ denotes respiratory
T10871 2304-2313 NNS denotes movements
T10873 2313-2314 . denotes .
T10874 2314-2375 sentence denotes With few exceptions, -/- pups died within 72 hours of birth.
T10875 2315-2319 IN denotes With
T10877 2320-2323 JJ denotes few
T10878 2324-2334 NNS denotes exceptions
T10879 2334-2336 , denotes ,
T10880 2336-2337 SYM denotes -
T10882 2337-2338 HYPH denotes /
T10881 2338-2339 SYM denotes -
T10883 2340-2344 NNS denotes pups
T10876 2345-2349 VBD denotes died
T10884 2350-2356 IN denotes within
T10885 2357-2359 CD denotes 72
T10886 2360-2365 NNS denotes hours
T10887 2366-2368 IN denotes of
T10888 2369-2374 NN denotes birth
T10889 2374-2375 . denotes .
T10890 2375-2444 sentence denotes We have observed one rare -/- pup that survived to postnatal day 13.
T10891 2376-2378 PRP denotes We
T10893 2379-2383 VBP denotes have
T10892 2384-2392 VBN denotes observed
T10894 2393-2396 CD denotes one
T10896 2397-2401 JJ denotes rare
T10897 2402-2403 SYM denotes -
T10899 2403-2404 HYPH denotes /
T10898 2404-2405 SYM denotes -
T10895 2406-2409 NN denotes pup
T10900 2410-2414 WDT denotes that
T10901 2415-2423 VBD denotes survived
T10902 2424-2426 IN denotes to
T10903 2427-2436 JJ denotes postnatal
T10904 2437-2440 NN denotes day
T10905 2441-2443 CD denotes 13
T10906 2443-2444 . denotes .
T10907 2444-2506 sentence denotes The reason for the prolonged survival of this pup is unknown.
T10908 2445-2448 DT denotes The
T10909 2449-2455 NN denotes reason
T10911 2456-2459 IN denotes for
T10912 2460-2463 DT denotes the
T10914 2464-2473 VBN denotes prolonged
T10913 2474-2482 NN denotes survival
T10915 2483-2485 IN denotes of
T10916 2486-2490 DT denotes this
T10917 2491-2494 NN denotes pup
T10910 2495-2497 VBZ denotes is
T10918 2498-2505 JJ denotes unknown
T10919 2505-2506 . denotes .
R2778 T10368 T10367 prep of,Production
R2779 T10369 T10370 compound E2,mice
R2780 T10370 T10368 pobj mice,of
R2781 T10371 T10372 compound gene,knockout
R2782 T10372 T10370 compound knockout,mice
R2783 T10374 T10375 aux To,create
R2784 T10375 T10376 advcl create,used
R2785 T10377 T10378 compound E2,mice
R2786 T10378 T10375 dobj mice,create
R2787 T10379 T10378 compound knockout,mice
R2788 T10380 T10376 punct ", ",used
R2789 T10381 T10376 nsubj we,used
R2790 T10382 T10383 compound gene,targeting
R2791 T10383 T10376 dobj targeting,used
R2792 T10384 T10376 prep in,used
R2793 T10385 T10386 compound mouse,cells
R2794 T10386 T10384 pobj cells,in
R2795 T10387 T10386 compound ES,cells
R2796 T10388 T10389 aux to,disrupt
R2797 T10389 T10376 advcl disrupt,used
R2798 T10390 T10391 det the,gene
R2799 T10391 T10389 dobj gene,disrupt
R2800 T10392 T10391 compound E2,gene
R2801 T10393 T10376 punct .,used
R2802 T10395 T10396 det The,strategy
R2803 T10396 T10398 nsubjpass strategy,illustrated
R2804 T10397 T10396 amod overall,strategy
R2805 T10399 T10396 prep for,strategy
R2806 T10400 T10399 pcomp disrupting,for
R2807 T10401 T10402 det the,gene
R2808 T10402 T10400 dobj gene,disrupting
R2809 T10403 T10402 compound E2,gene
R2810 T10404 T10398 auxpass is,illustrated
R2811 T10405 T10398 prep in,illustrated
R2812 T10406 T10407 compound Fig.,1A
R2813 T10407 T10405 pobj 1A,in
R2814 T10408 T10398 punct .,illustrated
R2815 T10410 T10411 det The,construct
R2816 T10411 T10414 nsubjpass construct,designed
R2817 T10412 T10413 compound gene,targeting
R2818 T10413 T10411 compound targeting,construct
R2819 T10415 T10414 auxpass was,designed
R2820 T10416 T10417 aux to,replace
R2821 T10417 T10414 advcl replace,designed
R2822 T10418 T10419 det a,fragment
R2823 T10419 T10417 dobj fragment,replace
R2824 T10420 T10421 nummod 1.67,kb
R2825 T10421 T10419 nmod kb,fragment
R2826 T10422 T10423 nmod EcoRV,Smal
R2827 T10423 T10419 nmod Smal,fragment
R2828 T10424 T10423 punct /,Smal
R2829 T10425 T10419 amod genomic,fragment
R2830 T10426 T10419 compound DNA,fragment
R2831 T10427 T10419 acl encompassing,fragment
R2832 T10428 T10427 dobj part,encompassing
R2833 T10429 T10428 prep of,part
R2834 T10430 T10429 pobj Exon,of
R2835 T10431 T10430 nummod 4,Exon
R2836 T10432 T10428 cc and,part
R2837 T10433 T10428 conj all,part
R2838 T10434 T10433 prep of,all
R2839 T10435 T10434 pobj Exon,of
R2840 T10436 T10435 nummod 5,Exon
R2841 T10437 T10417 prep with,replace
R2842 T10438 T10439 det the,cassette
R2843 T10439 T10437 pobj cassette,with
R2844 T10440 T10439 nmod PGKneo,cassette
R2845 T10441 T10439 amod selectable,cassette
R2846 T10442 T10439 compound marker,cassette
R2847 T10443 T10414 punct .,designed
R2848 T10445 T10446 prep Of,displayed
R2849 T10447 T10448 nummod 522,clones
R2850 T10448 T10445 pobj clones,Of
R2851 T10449 T10450 compound ES,cell
R2852 T10450 T10448 compound cell,clones
R2853 T10451 T10448 acl screened,clones
R2854 T10452 T10451 prep for,screened
R2855 T10453 T10452 pobj targeting,for
R2856 T10454 T10451 prep by,screened
R2857 T10455 T10456 compound Southern,blot
R2858 T10456 T10457 compound blot,analysis
R2859 T10457 T10454 pobj analysis,by
R2860 T10458 T10446 punct ", ",displayed
R2861 T10459 T10446 nsubj 29,displayed
R2862 T10460 T10461 punct (,%
R2863 T10461 T10459 parataxis %,29
R2864 T10462 T10461 nummod 5,%
R2865 T10463 T10461 punct ),%
R2866 T10464 T10465 det the,polymorphisms
R2867 T10465 T10446 dobj polymorphisms,displayed
R2868 T10466 T10465 amod predicted,polymorphisms
R2869 T10467 T10468 compound restriction,length
R2870 T10468 T10465 compound length,polymorphisms
R2871 T10469 T10468 compound fragment,length
R2872 T10470 T10465 amod indicative,polymorphisms
R2873 T10471 T10470 prep of,indicative
R2874 T10472 T10471 pobj gene,of
R2875 T10473 T10472 amod targeting,gene
R2876 T10474 T10446 prep at,displayed
R2877 T10475 T10476 det the,locus
R2878 T10476 T10474 pobj locus,at
R2879 T10477 T10476 compound E2,locus
R2880 T10478 T10446 punct .,displayed
R2881 T10480 T10481 mark As,illustrated
R2882 T10481 T10482 advcl illustrated,hybridizes
R2883 T10483 T10481 prep in,illustrated
R2884 T10484 T10485 compound Fig.,1A
R2885 T10485 T10483 pobj 1A,in
R2886 T10486 T10485 cc and,1A
R2887 T10487 T10485 conj 1B,1A
R2888 T10488 T10482 punct ", ",hybridizes
R2889 T10489 T10490 det an,probe
R2890 T10490 T10482 nsubj probe,hybridizes
R2891 T10491 T10490 nmod E2,probe
R2892 T10492 T10490 nmod Exon,probe
R2893 T10493 T10492 nummod 6,Exon
R2894 T10494 T10490 amod specific,probe
R2895 T10495 T10490 punct ", ",probe
R2896 T10496 T10497 dep which,is
R2897 T10497 T10490 relcl is,probe
R2898 T10498 T10497 acomp external,is
R2899 T10499 T10498 prep to,external
R2900 T10500 T10501 det the,construct
R2901 T10501 T10499 pobj construct,to
R2902 T10502 T10501 compound targeting,construct
R2903 T10503 T10482 punct ", ",hybridizes
R2904 T10504 T10482 prep to,hybridizes
R2905 T10505 T10506 advmod only,fragment
R2906 T10506 T10504 pobj fragment,to
R2907 T10507 T10506 det a,fragment
R2908 T10508 T10509 punct ~,16
R2909 T10509 T10510 nummod 16,kb
R2910 T10510 T10506 compound kb,fragment
R2911 T10511 T10506 compound BglI,fragment
R2912 T10512 T10506 compound restriction,fragment
R2913 T10513 T10506 prep from,fragment
R2914 T10514 T10515 det the,allele
R2915 T10515 T10513 pobj allele,from
R2916 T10516 T10517 amod wild,type
R2917 T10517 T10515 compound type,allele
R2918 T10518 T10515 prep in,allele
R2919 T10519 T10520 det the,line
R2920 T10520 T10518 pobj line,in
R2921 T10521 T10520 amod parental,line
R2922 T10522 T10520 compound R1,line
R2923 T10523 T10524 compound ES,cell
R2924 T10524 T10520 compound cell,line
R2925 T10525 T10482 punct .,hybridizes
R2926 T10527 T10528 prep In,hybridizes
R2927 T10529 T10530 advmod correctly,targeted
R2928 T10530 T10531 amod targeted,cells
R2929 T10531 T10527 pobj cells,In
R2930 T10532 T10531 compound ES,cells
R2931 T10533 T10528 punct ", ",hybridizes
R2932 T10534 T10535 det this,probe
R2933 T10535 T10528 nsubj probe,hybridizes
R2934 T10536 T10528 advmod also,hybridizes
R2935 T10537 T10528 prep to,hybridizes
R2936 T10538 T10539 det a,fragment
R2937 T10539 T10537 pobj fragment,to
R2938 T10540 T10541 punct ~,11
R2939 T10541 T10542 nummod 11,kb
R2940 T10542 T10539 compound kb,fragment
R2941 T10543 T10539 compound BglI,fragment
R2942 T10544 T10539 compound restriction,fragment
R2943 T10545 T10528 punct .,hybridizes
R2944 T10547 T10548 nsubjpass Targeting,confirmed
R2945 T10549 T10548 auxpass was,confirmed
R2946 T10550 T10548 prep with,confirmed
R2947 T10551 T10552 amod several,enzymes
R2948 T10552 T10550 pobj enzymes,with
R2949 T10553 T10552 amod additional,enzymes
R2950 T10554 T10552 compound restriction,enzymes
R2951 T10555 T10552 cc and,enzymes
R2952 T10556 T10552 conj probes,enzymes
R2953 T10557 T10558 punct (,shown
R2954 T10558 T10548 parataxis shown,confirmed
R2955 T10559 T10558 nsubj data,shown
R2956 T10560 T10558 neg not,shown
R2957 T10561 T10558 punct ),shown
R2958 T10562 T10548 punct .,confirmed
R2959 T10564 T10565 advmod Correctly,targeted
R2960 T10565 T10566 amod targeted,cells
R2961 T10566 T10568 nsubjpass cells,microinjected
R2962 T10567 T10566 compound ES,cells
R2963 T10569 T10568 auxpass were,microinjected
R2964 T10570 T10568 prep into,microinjected
R2965 T10571 T10570 pobj blastocysts,into
R2966 T10572 T10573 aux to,produce
R2967 T10573 T10568 advcl produce,microinjected
R2968 T10574 T10575 amod chimeric,mice
R2969 T10575 T10573 dobj mice,produce
R2970 T10576 T10568 punct .,microinjected
R2971 T10578 T10579 nsubjpass Chimerics,bred
R2972 T10580 T10579 auxpass were,bred
R2973 T10581 T10579 prep to,bred
R2974 T10582 T10583 compound C57BL,6J
R2975 T10583 T10585 compound 6J,mice
R2976 T10584 T10583 punct /,6J
R2977 T10585 T10581 pobj mice,to
R2978 T10586 T10579 punct .,bred
R2979 T10588 T10589 prep Following,interbred
R2980 T10590 T10591 compound germline,transmission
R2981 T10591 T10588 pobj transmission,Following
R2982 T10592 T10591 prep of,transmission
R2983 T10593 T10594 det the,allele
R2984 T10594 T10592 pobj allele,of
R2985 T10595 T10594 amod targeted,allele
R2986 T10596 T10589 punct ", ",interbred
R2987 T10597 T10598 amod heterozygous,mice
R2988 T10598 T10589 nsubjpass mice,interbred
R2989 T10599 T10589 auxpass were,interbred
R2990 T10600 T10601 aux to,produce
R2991 T10601 T10589 advcl produce,interbred
R2992 T10602 T10603 amod wild,type
R2993 T10603 T10604 nmod type,animals
R2994 T10604 T10601 dobj animals,produce
R2995 T10605 T10606 punct (,+
R2996 T10606 T10603 punct +,type
R2997 T10607 T10606 punct +,+
R2998 T10608 T10606 punct /,+
R2999 T10609 T10606 punct ),+
R3000 T10610 T10603 punct ", ",type
R3001 T10611 T10603 conj heterozygous,type
R3002 T10612 T10613 punct (,-
R3003 T10613 T10611 punct -,heterozygous
R3004 T10614 T10613 punct +,-
R3005 T10615 T10613 punct /,-
R3006 T10616 T10613 punct ),-
R3007 T10617 T10611 cc and,heterozygous
R3008 T10618 T10611 conj homozygous,heterozygous
R3009 T10619 T10620 punct (,-
R3010 T10620 T10618 punct -,homozygous
R3011 T10621 T10620 punct -,-
R3012 T10622 T10620 punct /,-
R3013 T10623 T10620 punct ),-
R3014 T10624 T10589 punct .,interbred
R3015 T10626 T10627 nsubjpass Mice,genotyped
R3016 T10628 T10627 auxpass were,genotyped
R3017 T10629 T10627 prep by,genotyped
R3018 T10630 T10631 compound Southern,blot
R3019 T10631 T10632 compound blot,analysis
R3020 T10632 T10629 pobj analysis,by
R3021 T10633 T10627 punct .,genotyped
R3022 T10635 T10636 det The,probe
R3023 T10636 T10640 nsubj probe,hybridized
R3024 T10637 T10638 npadvmod Exon,specific
R3025 T10638 T10636 amod specific,probe
R3026 T10639 T10637 nummod 6,Exon
R3027 T10641 T10640 prep to,hybridized
R3028 T10642 T10643 advmod only,fragment
R3029 T10643 T10641 pobj fragment,to
R3030 T10644 T10643 det a,fragment
R3031 T10645 T10646 punct ~,16
R3032 T10646 T10647 nummod 16,kb
R3033 T10647 T10643 compound kb,fragment
R3034 T10648 T10643 compound BglI,fragment
R3035 T10649 T10643 compound restriction,fragment
R3036 T10650 T10643 prep in,fragment
R3037 T10651 T10652 punct +,+
R3038 T10652 T10654 punct +,mice
R3039 T10653 T10652 punct /,+
R3040 T10654 T10650 pobj mice,in
R3041 T10655 T10643 punct ", ",fragment
R3042 T10656 T10657 det a,fragment
R3043 T10657 T10643 appos fragment,fragment
R3044 T10658 T10659 punct ~,11
R3045 T10659 T10660 nummod 11,kb
R3046 T10660 T10657 compound kb,fragment
R3047 T10661 T10657 compound BglI,fragment
R3048 T10662 T10657 prep in,fragment
R3049 T10663 T10664 punct -,-
R3050 T10664 T10666 punct -,mice
R3051 T10665 T10664 punct /,-
R3052 T10666 T10662 pobj mice,in
R3053 T10667 T10641 punct ", ",to
R3054 T10668 T10641 cc and,to
R3055 T10669 T10641 conj to,to
R3056 T10670 T10669 pobj both,to
R3057 T10671 T10670 prep of,both
R3058 T10672 T10673 det these,fragments
R3059 T10673 T10671 pobj fragments,of
R3060 T10674 T10670 prep in,both
R3061 T10675 T10676 punct +,-
R3062 T10676 T10678 punct -,mice
R3063 T10677 T10676 punct /,-
R3064 T10678 T10674 pobj mice,in
R3065 T10679 T10680 punct (,1B
R3066 T10680 T10640 parataxis 1B,hybridized
R3067 T10681 T10680 compound Fig.,1B
R3068 T10682 T10680 punct ),1B
R3069 T10683 T10640 punct .,hybridized
R3070 T10685 T10686 nsubjpass Mice,born
R3071 T10687 T10685 amod homozygous,Mice
R3072 T10688 T10687 prep for,homozygous
R3073 T10689 T10690 det the,mutation
R3074 T10690 T10688 pobj mutation,for
R3075 T10691 T10690 compound E2,mutation
R3076 T10692 T10686 auxpass were,born
R3077 T10693 T10686 prep at,born
R3078 T10694 T10695 det the,frequency
R3079 T10695 T10693 pobj frequency,at
R3080 T10696 T10695 amod expected,frequency
R3081 T10697 T10686 punct .,born
R3082 T10699 T10700 compound Genotype,analysis
R3083 T10700 T10701 nsubj analysis,revealed
R3084 T10702 T10700 prep of,analysis
R3085 T10703 T10702 pobj pups,of
R3086 T10704 T10703 acl derived,pups
R3087 T10705 T10704 prep from,derived
R3088 T10706 T10707 punct +,-
R3089 T10707 T10709 punct -,matings
R3090 T10708 T10707 punct /,-
R3091 T10709 T10705 pobj matings,from
R3092 T10710 T10707 prep by,-
R3093 T10711 T10712 punct +,-
R3094 T10712 T10710 punct -,by
R3095 T10713 T10712 punct /,-
R3096 T10714 T10715 mark that,were
R3097 T10715 T10701 ccomp were,revealed
R3098 T10716 T10717 punct +,+
R3099 T10717 T10719 punct +,mice
R3100 T10718 T10717 punct /,+
R3101 T10719 T10715 nsubj mice,were
R3102 T10720 T10717 punct ", ",+
R3103 T10721 T10722 punct +,-
R3104 T10722 T10717 conj -,+
R3105 T10723 T10722 punct /,-
R3106 T10724 T10722 punct ", ",-
R3107 T10725 T10722 cc and,-
R3108 T10726 T10727 punct -,-
R3109 T10727 T10722 conj -,-
R3110 T10728 T10727 punct /,-
R3111 T10729 T10715 acomp present,were
R3112 T10730 T10715 prep at,were
R3113 T10731 T10732 advmod nearly,frequency
R3114 T10732 T10730 pobj frequency,at
R3115 T10733 T10732 det the,frequency
R3116 T10734 T10732 amod expected,frequency
R3117 T10735 T10736 nummod 1,1
R3118 T10736 T10732 nummod 1,frequency
R3119 T10737 T10736 punct :,1
R3120 T10738 T10736 nummod 2,1
R3121 T10739 T10736 punct :,1
R3122 T10740 T10701 punct .,revealed
R3123 T10742 T10743 prep Of,were
R3124 T10744 T10745 det the,animals
R3125 T10745 T10742 pobj animals,Of
R3126 T10746 T10745 amod initial,animals
R3127 T10747 T10745 nummod 60,animals
R3128 T10748 T10745 acl genotyped,animals
R3129 T10749 T10743 punct ", ",were
R3130 T10750 T10743 nsubj 19,were
R3131 T10751 T10752 punct (,%
R3132 T10752 T10750 parataxis %,19
R3133 T10753 T10752 nummod 32,%
R3134 T10754 T10752 punct ),%
R3135 T10755 T10756 punct +,+
R3136 T10756 T10743 attr +,were
R3137 T10757 T10756 punct /,+
R3138 T10758 T10743 punct ", ",were
R3139 T10759 T10760 nsubj 27,were
R3140 T10760 T10743 conj were,were
R3141 T10761 T10762 punct (,%
R3142 T10762 T10759 parataxis %,27
R3143 T10763 T10762 nummod 45,%
R3144 T10764 T10762 punct ),%
R3145 T10765 T10766 punct +,-
R3146 T10766 T10760 attr -,were
R3147 T10767 T10766 punct /,-
R3148 T10768 T10760 punct ", ",were
R3149 T10769 T10760 cc and,were
R3150 T10770 T10771 nsubj 14,were
R3151 T10771 T10760 conj were,were
R3152 T10772 T10773 punct (,%
R3153 T10773 T10770 parataxis %,14
R3154 T10774 T10773 nummod 23,%
R3155 T10775 T10773 punct ),%
R3156 T10776 T10777 punct -,-
R3157 T10777 T10771 attr -,were
R3158 T10778 T10777 punct /,-
R3159 T10779 T10743 punct .,were
R3160 T10781 T10782 advmod Thus,was
R3161 T10783 T10782 punct ", ",was
R3162 T10784 T10785 det the,gene
R3163 T10785 T10782 nsubj gene,was
R3164 T10786 T10785 compound E2,gene
R3165 T10787 T10782 acomp dispensable,was
R3166 T10788 T10787 prep for,dispensable
R3167 T10789 T10790 amod normal,development
R3168 T10790 T10788 pobj development,for
R3169 T10791 T10790 amod embryonic,development
R3170 T10792 T10782 punct .,was
R3171 T10794 T10795 advmod However,died
R3172 T10796 T10795 punct ", ",died
R3173 T10797 T10798 mark as,expected
R3174 T10798 T10795 advcl expected,died
R3175 T10799 T10795 punct ", ",died
R3176 T10800 T10801 advmod nearly,mice
R3177 T10801 T10795 nsubj mice,died
R3178 T10802 T10801 det all,mice
R3179 T10803 T10801 amod homozygous,mice
R3180 T10804 T10795 prep in,died
R3181 T10805 T10806 det the,period
R3182 T10806 T10804 pobj period,in
R3183 T10807 T10806 amod perinatal,period
R3184 T10808 T10795 punct .,died
R3185 T10810 T10811 advmod Immediately,following
R3186 T10811 T10812 prep following,were
R3187 T10812 T10817 ccomp were,were
R3188 T10813 T10811 pobj birth,following
R3189 T10814 T10812 punct ", ",were
R3190 T10815 T10816 amod homozygous,pups
R3191 T10816 T10812 nsubj pups,were
R3192 T10818 T10812 acomp indistinguishable,were
R3193 T10819 T10818 prep from,indistinguishable
R3194 T10820 T10821 poss their,littermates
R3195 T10821 T10819 pobj littermates,from
R3196 T10822 T10823 punct +,+
R3197 T10823 T10821 punct +,littermates
R3198 T10824 T10823 punct /,+
R3199 T10825 T10823 cc and,+
R3200 T10826 T10827 punct +,-
R3201 T10827 T10823 conj -,+
R3202 T10828 T10827 punct /,-
R3203 T10829 T10817 punct ;,were
R3204 T10830 T10817 nsubj they,were
R3205 T10831 T10817 acomp vigorous,were
R3206 T10832 T10831 punct ", ",vigorous
R3207 T10833 T10831 conj active,vigorous
R3208 T10834 T10833 cc and,active
R3209 T10835 T10833 conj able,active
R3210 T10836 T10837 aux to,suckle
R3211 T10837 T10835 xcomp suckle,able
R3212 T10838 T10817 punct .,were
R3213 T10840 T10841 prep By,became
R3214 T10842 T10843 amod mid,day
R3215 T10843 T10840 pobj day,By
R3216 T10844 T10842 prep to,mid
R3217 T10845 T10844 amod late,to
R3218 T10846 T10843 prep on,day
R3219 T10847 T10848 amod postnatal,day
R3220 T10848 T10846 pobj day,on
R3221 T10849 T10848 nummod one,day
R3222 T10850 T10841 punct ", ",became
R3223 T10851 T10852 advmod most,pups
R3224 T10852 T10841 nsubj pups,became
R3225 T10853 T10854 punct -,-
R3226 T10854 T10852 punct -,pups
R3227 T10855 T10854 punct /,-
R3228 T10856 T10841 acomp moribund,became
R3229 T10857 T10841 cc and,became
R3230 T10858 T10841 conj were,became
R3231 T10859 T10860 advmod readily,identifiable
R3232 T10860 T10858 acomp identifiable,were
R3233 T10861 T10862 mark as,were
R3234 T10862 T10860 advcl were,identifiable
R3235 T10863 T10862 nsubj they,were
R3236 T10864 T10865 amod lethargic,pale
R3237 T10865 T10862 acomp pale,were
R3238 T10866 T10865 punct ", ",pale
R3239 T10867 T10862 punct ", ",were
R3240 T10868 T10862 cc and,were
R3241 T10869 T10862 conj exhibited,were
R3242 T10870 T10871 amod gasping,movements
R3243 T10871 T10869 dobj movements,exhibited
R3244 T10872 T10871 amod respiratory,movements
R3245 T10873 T10841 punct .,became
R3246 T10875 T10876 prep With,died
R3247 T10877 T10878 amod few,exceptions
R3248 T10878 T10875 pobj exceptions,With
R3249 T10879 T10876 punct ", ",died
R3250 T10880 T10881 punct -,-
R3251 T10881 T10883 punct -,pups
R3252 T10882 T10881 punct /,-
R3253 T10883 T10876 nsubj pups,died
R3254 T10884 T10876 prep within,died
R3255 T10885 T10886 nummod 72,hours
R3256 T10886 T10884 pobj hours,within
R3257 T10887 T10886 prep of,hours
R3258 T10888 T10887 pobj birth,of
R3259 T10889 T10876 punct .,died
R3260 T10891 T10892 nsubj We,observed
R3261 T10893 T10892 aux have,observed
R3262 T10894 T10895 nummod one,pup
R3263 T10895 T10892 dobj pup,observed
R3264 T10896 T10895 amod rare,pup
R3265 T10897 T10898 punct -,-
R3266 T10898 T10895 punct -,pup
R3267 T10899 T10898 punct /,-
R3268 T10900 T10901 dep that,survived
R3269 T10901 T10895 relcl survived,pup
R3270 T10902 T10901 prep to,survived
R3271 T10903 T10904 amod postnatal,day
R3272 T10904 T10902 pobj day,to
R3273 T10905 T10904 nummod 13,day
R3274 T10906 T10892 punct .,observed
R3275 T10908 T10909 det The,reason
R3276 T10909 T10910 nsubj reason,is
R3277 T10911 T10909 prep for,reason
R3278 T10912 T10913 det the,survival
R3279 T10913 T10911 pobj survival,for
R3280 T10914 T10913 amod prolonged,survival
R3281 T10915 T10913 prep of,survival
R3282 T10916 T10917 det this,pup
R3283 T10917 T10915 pobj pup,of
R3284 T10918 T10910 acomp unknown,is
R3285 T10919 T10910 punct .,is