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PMC:1448208 / 12579-13664 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T7185 0-7 JJ denotes Primary
T7187 8-13 NN denotes mouse
T7188 14-23 JJ denotes embryonic
T7186 24-35 NNS denotes fibroblasts
T7190 36-40 VBD denotes were
T7189 41-49 VBN denotes prepared
T7191 50-54 IN denotes from
T7192 55-64 JJ denotes embryonic
T7194 65-68 NN denotes day
T7196 69-70 SYM denotes ~
T7195 70-74 CD denotes 16.5
T7197 74-75 SYM denotes –
T7198 75-79 CD denotes 18.5
T7193 80-87 NNS denotes fetuses
T7199 88-90 IN denotes as
T7200 91-100 VBN denotes described
T7201 101-102 -LRB- denotes [
T7202 102-104 CD denotes 29
T7203 104-105 -RRB- denotes ]
T7204 105-106 . denotes .
T7205 106-362 sentence denotes Fibroblasts were passed onto glass, fixed in 2% paraformaldehyde in PBS for 10 minutes, permeabilized in 2% paraformaldehyde containing 0.1% Triton X100 for 10 minutes and washed three times in PBS containing 0.5% BSA and 0.15% glycine, pH 7.4 (Buffer A).
T7206 107-118 NNS denotes Fibroblasts
T7208 119-123 VBD denotes were
T7207 124-130 VBN denotes passed
T7209 131-135 IN denotes onto
T7210 136-141 NN denotes glass
T7211 141-143 , denotes ,
T7212 143-148 VBN denotes fixed
T7213 149-151 IN denotes in
T7214 152-153 CD denotes 2
T7215 153-154 NN denotes %
T7216 155-171 NN denotes paraformaldehyde
T7217 172-174 IN denotes in
T7218 175-178 NN denotes PBS
T7219 179-182 IN denotes for
T7220 183-185 CD denotes 10
T7221 186-193 NNS denotes minutes
T7222 193-195 , denotes ,
T7223 195-208 VBN denotes permeabilized
T7224 209-211 IN denotes in
T7225 212-213 CD denotes 2
T7226 213-214 NN denotes %
T7227 215-231 NN denotes paraformaldehyde
T7228 232-242 VBG denotes containing
T7229 243-246 CD denotes 0.1
T7230 246-247 NN denotes %
T7232 248-254 NN denotes Triton
T7231 255-259 NN denotes X100
T7233 260-263 IN denotes for
T7234 264-266 CD denotes 10
T7235 267-274 NNS denotes minutes
T7236 275-278 CC denotes and
T7237 279-285 VBD denotes washed
T7238 286-291 CD denotes three
T7239 292-297 NNS denotes times
T7240 298-300 IN denotes in
T7241 301-304 NN denotes PBS
T7242 305-315 VBG denotes containing
T7243 316-319 CD denotes 0.5
T7244 319-320 NN denotes %
T7245 321-324 NN denotes BSA
T7246 325-328 CC denotes and
T7247 329-333 CD denotes 0.15
T7248 333-334 NN denotes %
T7249 335-342 NN denotes glycine
T7250 342-344 , denotes ,
T7251 344-346 NN denotes pH
T7252 347-350 CD denotes 7.4
T7253 351-352 -LRB- denotes (
T7255 352-358 NN denotes Buffer
T7254 359-360 NN denotes A
T7256 360-361 -RRB- denotes )
T7257 361-362 . denotes .
T7258 362-669 sentence denotes Following a 30 min incubation with purified goat IgG (50 (μg/ml) at 25°C and three additional washes with Buffer A, cells were incubated for 60 min with E2-specific antiserum [30] at 1 μg/ml followed by three washes in Buffer A and 60 minute incubation in fluorescently labeled second antibody (1–2 μg/ml).
T7259 363-372 VBG denotes Following
T7261 373-374 DT denotes a
T7263 375-377 CD denotes 30
T7264 378-381 NN denotes min
T7262 382-392 NN denotes incubation
T7265 393-397 IN denotes with
T7266 398-406 VBN denotes purified
T7268 407-411 NN denotes goat
T7267 412-415 NN denotes IgG
T7269 416-417 -LRB- denotes (
T7271 417-419 CD denotes 50
T7272 420-421 -LRB- denotes (
T7270 421-423 NN denotes μg
T7273 423-424 SYM denotes /
T7274 424-426 NN denotes ml
T7275 426-427 -RRB- denotes )
T7276 428-430 IN denotes at
T7277 431-433 CD denotes 25
T7278 433-435 NN denotes °C
T7279 436-439 CC denotes and
T7280 440-445 CD denotes three
T7282 446-456 JJ denotes additional
T7281 457-463 NNS denotes washes
T7283 464-468 IN denotes with
T7284 469-475 NN denotes Buffer
T7285 476-477 NN denotes A
T7286 477-479 , denotes ,
T7287 479-484 NNS denotes cells
T7288 485-489 VBD denotes were
T7260 490-499 VBN denotes incubated
T7289 500-503 IN denotes for
T7290 504-506 CD denotes 60
T7291 507-510 NN denotes min
T7292 511-515 IN denotes with
T7293 516-518 NN denotes E2
T7295 518-519 HYPH denotes -
T7294 519-527 JJ denotes specific
T7296 528-537 NN denotes antiserum
T7297 538-539 -LRB- denotes [
T7298 539-541 CD denotes 30
T7299 541-542 -RRB- denotes ]
T7300 543-545 IN denotes at
T7301 546-547 CD denotes 1
T7302 548-550 NN denotes μg
T7303 550-551 SYM denotes /
T7304 551-553 NN denotes ml
T7305 554-562 VBN denotes followed
T7306 563-565 IN denotes by
T7307 566-571 CD denotes three
T7308 572-578 NNS denotes washes
T7309 579-581 IN denotes in
T7310 582-588 NN denotes Buffer
T7311 589-590 NN denotes A
T7312 591-594 CC denotes and
T7313 595-597 CD denotes 60
T7314 598-604 NN denotes minute
T7315 605-615 NN denotes incubation
T7316 616-618 IN denotes in
T7317 619-632 RB denotes fluorescently
T7318 633-640 VBN denotes labeled
T7320 641-647 JJ denotes second
T7319 648-656 NN denotes antibody
T7321 657-658 -LRB- denotes (
T7323 658-659 CD denotes 1
T7325 659-660 SYM denotes –
T7324 660-661 CD denotes 2
T7322 662-664 NN denotes μg
T7326 664-665 SYM denotes /
T7327 665-667 NN denotes ml
T7328 667-668 -RRB- denotes )
T7329 668-669 . denotes .
T7330 669-782 sentence denotes The cells were then washed six times (5 min/wash) in Buffer A and then mounted in gelvatol (Monsanto, St Louis).
T7331 670-673 DT denotes The
T7332 674-679 NNS denotes cells
T7334 680-684 VBD denotes were
T7335 685-689 RB denotes then
T7333 690-696 VBN denotes washed
T7336 697-700 CD denotes six
T7337 701-706 NNS denotes times
T7338 707-708 -LRB- denotes (
T7340 708-709 CD denotes 5
T7339 710-713 NN denotes min
T7341 713-714 SYM denotes /
T7342 714-718 NN denotes wash
T7343 718-719 -RRB- denotes )
T7344 720-722 IN denotes in
T7345 723-729 NN denotes Buffer
T7346 730-731 NN denotes A
T7347 732-735 CC denotes and
T7348 736-740 RB denotes then
T7349 741-748 VBN denotes mounted
T7350 749-751 IN denotes in
T7351 752-760 NN denotes gelvatol
T7352 761-762 -LRB- denotes (
T7353 762-770 NNP denotes Monsanto
T7354 770-772 , denotes ,
T7355 772-774 NNP denotes St
T7356 775-780 NNP denotes Louis
T7357 780-781 -RRB- denotes )
T7358 781-782 . denotes .
T7359 782-987 sentence denotes When livers from newborn pups were examined, fixation was by immersion in 2% paraformaldehyde followed by cryoprotection and shock freezing in liquid nitrogen cooled isopentane and sectioning (5 microns).
T7360 783-787 WRB denotes When
T7362 788-794 NNS denotes livers
T7363 795-799 IN denotes from
T7364 800-807 JJ denotes newborn
T7365 808-812 NNS denotes pups
T7366 813-817 VBD denotes were
T7361 818-826 VBN denotes examined
T7368 826-828 , denotes ,
T7369 828-836 NN denotes fixation
T7367 837-840 VBD denotes was
T7370 841-843 IN denotes by
T7371 844-853 NN denotes immersion
T7372 854-856 IN denotes in
T7373 857-858 CD denotes 2
T7374 858-859 NN denotes %
T7375 860-876 NN denotes paraformaldehyde
T7376 877-885 VBN denotes followed
T7377 886-888 IN denotes by
T7378 889-903 NN denotes cryoprotection
T7379 904-907 CC denotes and
T7380 908-913 NN denotes shock
T7381 914-922 NN denotes freezing
T7382 923-925 IN denotes in
T7383 926-932 JJ denotes liquid
T7384 933-941 NN denotes nitrogen
T7385 942-948 VBN denotes cooled
T7386 949-959 NN denotes isopentane
T7387 960-963 CC denotes and
T7388 964-974 NN denotes sectioning
T7389 975-976 -LRB- denotes (
T7391 976-977 CD denotes 5
T7390 978-985 NNS denotes microns
T7392 985-986 -RRB- denotes )
T7393 986-987 . denotes .
T7394 987-1085 sentence denotes Otherwise processing was as for the cells above (without the fixation and permabilization steps).
T7395 988-997 RB denotes Otherwise
T7397 998-1008 NN denotes processing
T7396 1009-1012 VBD denotes was
T7398 1013-1015 IN denotes as
T7399 1016-1019 IN denotes for
T7400 1020-1023 DT denotes the
T7401 1024-1029 NNS denotes cells
T7402 1030-1035 RB denotes above
T7403 1036-1037 -LRB- denotes (
T7404 1037-1044 IN denotes without
T7405 1045-1048 DT denotes the
T7407 1049-1057 NN denotes fixation
T7408 1058-1061 CC denotes and
T7409 1062-1077 NN denotes permabilization
T7406 1078-1083 NNS denotes steps
T7410 1083-1084 -RRB- denotes )
T7411 1084-1085 . denotes .
R2009 T7185 T7186 amod Primary,fibroblasts
R2010 T7186 T7189 nsubjpass fibroblasts,prepared
R2011 T7187 T7186 nmod mouse,fibroblasts
R2012 T7188 T7186 amod embryonic,fibroblasts
R2013 T7190 T7189 auxpass were,prepared
R2014 T7191 T7189 prep from,prepared
R2015 T7192 T7193 amod embryonic,fetuses
R2016 T7193 T7191 pobj fetuses,from
R2017 T7194 T7195 nmod day,16.5
R2018 T7195 T7193 nummod 16.5,fetuses
R2019 T7196 T7195 punct ~,16.5
R2020 T7197 T7198 punct –,18.5
R2021 T7198 T7195 prep 18.5,16.5
R2022 T7199 T7200 mark as,described
R2023 T7200 T7189 advcl described,prepared
R2024 T7201 T7202 punct [,29
R2025 T7202 T7189 parataxis 29,prepared
R2026 T7203 T7202 punct ],29
R2027 T7204 T7189 punct .,prepared
R2028 T7206 T7207 nsubjpass Fibroblasts,passed
R2029 T7208 T7207 auxpass were,passed
R2030 T7209 T7207 prep onto,passed
R2031 T7210 T7209 pobj glass,onto
R2032 T7211 T7207 punct ", ",passed
R2033 T7212 T7207 conj fixed,passed
R2034 T7213 T7212 prep in,fixed
R2035 T7214 T7215 nummod 2,%
R2036 T7215 T7216 compound %,paraformaldehyde
R2037 T7216 T7213 pobj paraformaldehyde,in
R2038 T7217 T7212 prep in,fixed
R2039 T7218 T7217 pobj PBS,in
R2040 T7219 T7212 prep for,fixed
R2041 T7220 T7221 nummod 10,minutes
R2042 T7221 T7219 pobj minutes,for
R2043 T7222 T7212 punct ", ",fixed
R2044 T7223 T7212 conj permeabilized,fixed
R2045 T7224 T7223 prep in,permeabilized
R2046 T7225 T7226 nummod 2,%
R2047 T7226 T7227 compound %,paraformaldehyde
R2048 T7227 T7224 pobj paraformaldehyde,in
R2049 T7228 T7227 acl containing,paraformaldehyde
R2050 T7229 T7230 nummod 0.1,%
R2051 T7230 T7231 compound %,X100
R2052 T7231 T7228 dobj X100,containing
R2053 T7232 T7231 compound Triton,X100
R2054 T7233 T7223 prep for,permeabilized
R2055 T7234 T7235 nummod 10,minutes
R2056 T7235 T7233 pobj minutes,for
R2057 T7236 T7223 cc and,permeabilized
R2058 T7237 T7223 conj washed,permeabilized
R2059 T7238 T7239 nummod three,times
R2060 T7239 T7237 npadvmod times,washed
R2061 T7240 T7237 prep in,washed
R2062 T7241 T7240 pobj PBS,in
R2063 T7242 T7241 acl containing,PBS
R2064 T7243 T7244 nummod 0.5,%
R2065 T7244 T7245 compound %,BSA
R2066 T7245 T7242 dobj BSA,containing
R2067 T7246 T7245 cc and,BSA
R2068 T7247 T7248 nummod 0.15,%
R2069 T7248 T7249 compound %,glycine
R2070 T7249 T7245 conj glycine,BSA
R2071 T7250 T7242 punct ", ",containing
R2072 T7251 T7242 npadvmod pH,containing
R2073 T7252 T7251 nummod 7.4,pH
R2074 T7253 T7254 punct (,A
R2075 T7254 T7242 parataxis A,containing
R2076 T7255 T7254 compound Buffer,A
R2077 T7256 T7254 punct ),A
R2078 T7257 T7207 punct .,passed
R2079 T7259 T7260 prep Following,incubated
R2080 T7261 T7262 det a,incubation
R2081 T7262 T7259 pobj incubation,Following
R2082 T7263 T7264 nummod 30,min
R2083 T7264 T7262 compound min,incubation
R2084 T7265 T7262 prep with,incubation
R2085 T7266 T7267 amod purified,IgG
R2086 T7267 T7265 pobj IgG,with
R2087 T7268 T7267 compound goat,IgG
R2088 T7269 T7270 punct (,μg
R2089 T7270 T7267 parataxis μg,IgG
R2090 T7271 T7270 nummod 50,μg
R2091 T7272 T7270 punct (,μg
R2092 T7273 T7274 punct /,ml
R2093 T7274 T7270 prep ml,μg
R2094 T7275 T7270 punct ),μg
R2095 T7276 T7262 prep at,incubation
R2096 T7277 T7278 nummod 25,°C
R2097 T7278 T7276 pobj °C,at
R2098 T7279 T7262 cc and,incubation
R2099 T7280 T7281 nummod three,washes
R2100 T7281 T7262 conj washes,incubation
R2101 T7282 T7281 amod additional,washes
R2102 T7283 T7281 prep with,washes
R2103 T7284 T7285 compound Buffer,A
R2104 T7285 T7283 pobj A,with
R2105 T7286 T7260 punct ", ",incubated
R2106 T7287 T7260 nsubjpass cells,incubated
R2107 T7288 T7260 auxpass were,incubated
R2108 T7289 T7260 prep for,incubated
R2109 T7290 T7291 nummod 60,min
R2110 T7291 T7289 pobj min,for
R2111 T7292 T7260 prep with,incubated
R2112 T7293 T7294 npadvmod E2,specific
R2113 T7294 T7296 amod specific,antiserum
R2114 T7295 T7294 punct -,specific
R2115 T7296 T7292 pobj antiserum,with
R2116 T7297 T7298 punct [,30
R2117 T7298 T7296 parataxis 30,antiserum
R2118 T7299 T7298 punct ],30
R2119 T7300 T7296 prep at,antiserum
R2120 T7301 T7302 nummod 1,μg
R2121 T7302 T7300 pobj μg,at
R2122 T7303 T7304 punct /,ml
R2123 T7304 T7302 prep ml,μg
R2124 T7305 T7260 advcl followed,incubated
R2125 T7306 T7305 agent by,followed
R2126 T7307 T7308 nummod three,washes
R2127 T7308 T7306 pobj washes,by
R2128 T7309 T7308 prep in,washes
R2129 T7310 T7311 compound Buffer,A
R2130 T7311 T7309 pobj A,in
R2131 T7312 T7308 cc and,washes
R2132 T7313 T7314 nummod 60,minute
R2133 T7314 T7315 compound minute,incubation
R2134 T7315 T7308 conj incubation,washes
R2135 T7316 T7315 prep in,incubation
R2136 T7317 T7318 advmod fluorescently,labeled
R2137 T7318 T7319 amod labeled,antibody
R2138 T7319 T7316 pobj antibody,in
R2139 T7320 T7319 amod second,antibody
R2140 T7321 T7322 punct (,μg
R2141 T7322 T7260 parataxis μg,incubated
R2142 T7323 T7324 quantmod 1,2
R2143 T7324 T7322 nummod 2,μg
R2144 T7325 T7324 punct –,2
R2145 T7326 T7327 punct /,ml
R2146 T7327 T7322 prep ml,μg
R2147 T7328 T7322 punct ),μg
R2148 T7329 T7260 punct .,incubated
R2149 T7331 T7332 det The,cells
R2150 T7332 T7333 nsubjpass cells,washed
R2151 T7334 T7333 auxpass were,washed
R2152 T7335 T7333 advmod then,washed
R2153 T7336 T7337 nummod six,times
R2154 T7337 T7333 npadvmod times,washed
R2155 T7338 T7339 punct (,min
R2156 T7339 T7337 parataxis min,times
R2157 T7340 T7339 nummod 5,min
R2158 T7341 T7342 punct /,wash
R2159 T7342 T7339 prep wash,min
R2160 T7343 T7339 punct ),min
R2161 T7344 T7333 prep in,washed
R2162 T7345 T7346 compound Buffer,A
R2163 T7346 T7344 pobj A,in
R2164 T7347 T7333 cc and,washed
R2165 T7348 T7349 advmod then,mounted
R2166 T7349 T7333 conj mounted,washed
R2167 T7350 T7349 prep in,mounted
R2168 T7351 T7350 pobj gelvatol,in
R2169 T7352 T7353 punct (,Monsanto
R2170 T7353 T7351 parataxis Monsanto,gelvatol
R2171 T7354 T7353 punct ", ",Monsanto
R2172 T7355 T7356 compound St,Louis
R2173 T7356 T7353 npadvmod Louis,Monsanto
R2174 T7357 T7353 punct ),Monsanto
R2175 T7358 T7333 punct .,washed
R2176 T7360 T7361 advmod When,examined
R2177 T7361 T7367 advcl examined,was
R2178 T7362 T7361 nsubjpass livers,examined
R2179 T7363 T7362 prep from,livers
R2180 T7364 T7365 amod newborn,pups
R2181 T7365 T7363 pobj pups,from
R2182 T7366 T7361 auxpass were,examined
R2183 T7368 T7367 punct ", ",was
R2184 T7369 T7367 nsubj fixation,was
R2185 T7370 T7367 prep by,was
R2186 T7371 T7370 pobj immersion,by
R2187 T7372 T7371 prep in,immersion
R2188 T7373 T7374 nummod 2,%
R2189 T7374 T7375 compound %,paraformaldehyde
R2190 T7375 T7372 pobj paraformaldehyde,in
R2191 T7376 T7371 acl followed,immersion
R2192 T7377 T7376 agent by,followed
R2193 T7378 T7377 pobj cryoprotection,by
R2194 T7379 T7378 cc and,cryoprotection
R2195 T7380 T7381 compound shock,freezing
R2196 T7381 T7378 conj freezing,cryoprotection
R2197 T7382 T7378 prep in,cryoprotection
R2198 T7383 T7384 amod liquid,nitrogen
R2199 T7384 T7385 npadvmod nitrogen,cooled
R2200 T7385 T7386 amod cooled,isopentane
R2201 T7386 T7382 pobj isopentane,in
R2202 T7387 T7378 cc and,cryoprotection
R2203 T7388 T7378 conj sectioning,cryoprotection
R2204 T7389 T7390 punct (,microns
R2205 T7390 T7388 parataxis microns,sectioning
R2206 T7391 T7390 nummod 5,microns
R2207 T7392 T7390 punct ),microns
R2208 T7393 T7367 punct .,was
R2209 T7395 T7396 advmod Otherwise,was
R2210 T7397 T7396 nsubj processing,was
R2211 T7398 T7396 prep as,was
R2212 T7399 T7398 prep for,as
R2213 T7400 T7401 det the,cells
R2214 T7401 T7399 pobj cells,for
R2215 T7402 T7401 advmod above,cells
R2216 T7403 T7396 punct (,was
R2217 T7404 T7396 prep without,was
R2218 T7405 T7406 det the,steps
R2219 T7406 T7404 pobj steps,without
R2220 T7407 T7406 nmod fixation,steps
R2221 T7408 T7407 cc and,fixation
R2222 T7409 T7407 conj permabilization,fixation
R2223 T7410 T7396 punct ),was
R2224 T7411 T7396 punct .,was