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PMC:1448208 / 10298-11008 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T5877 0-8 JJ denotes Standard
T5879 9-18 JJ denotes molecular
T5878 19-29 NNS denotes techniques
T5881 30-34 VBD denotes were
T5880 35-39 VBN denotes used
T5882 40-42 TO denotes to
T5883 43-51 VB denotes assemble
T5884 52-55 DT denotes the
T5886 56-66 JJ denotes transgenic
T5885 67-76 NN denotes construct
T5887 76-78 , denotes ,
T5888 78-82 NN denotes pTRE
T5890 82-83 HYPH denotes -
T5889 83-85 NN denotes E2
T5891 85-86 . denotes .
T5892 86-710 sentence denotes This transgene contains the tetracycline responsive hCMV*-1 promoter [consisting of the tetracycline responsive element (TRE) and a minimal hCMV promoter] [25] from pTRE2 (Clontech Inc., Mt. View, CA), a chimeric intron from pCI (Promega) to increase message stability and expression [26,27], a Kozak consensus sequence at the initiation codon to optimize translation [28], the human E2 cDNA which has been modified to contain a 4× alanine linker followed by a c-myc epitope tag at the carboxy terminus to facilitate detection, and an SV40 late polyadenylation sequence from pCI for enhanced mRNA stability and translation.
T5893 87-91 DT denotes This
T5894 92-101 NN denotes transgene
T5895 102-110 VBZ denotes contains
T5896 111-114 DT denotes the
T5898 115-127 NN denotes tetracycline
T5899 128-138 JJ denotes responsive
T5900 139-143 NN denotes hCMV
T5901 143-144 SYM denotes *
T5902 144-145 HYPH denotes -
T5903 145-146 CD denotes 1
T5897 147-155 NN denotes promoter
T5904 156-157 -LRB- denotes [
T5905 157-167 VBG denotes consisting
T5906 168-170 IN denotes of
T5907 171-174 DT denotes the
T5909 175-187 NN denotes tetracycline
T5910 188-198 JJ denotes responsive
T5908 199-206 NN denotes element
T5911 207-208 -LRB- denotes (
T5912 208-211 NN denotes TRE
T5913 211-212 -RRB- denotes )
T5914 213-216 CC denotes and
T5915 217-218 DT denotes a
T5917 219-226 JJ denotes minimal
T5918 227-231 NN denotes hCMV
T5916 232-240 NN denotes promoter
T5919 240-241 -RRB- denotes ]
T5920 242-243 -LRB- denotes [
T5921 243-245 CD denotes 25
T5922 245-246 -RRB- denotes ]
T5923 247-251 IN denotes from
T5924 252-257 NN denotes pTRE2
T5925 258-259 -LRB- denotes (
T5927 259-267 NNP denotes Clontech
T5926 268-272 NNP denotes Inc.
T5928 272-274 , denotes ,
T5929 274-277 NNP denotes Mt.
T5930 278-282 NNP denotes View
T5931 282-284 , denotes ,
T5932 284-286 NNP denotes CA
T5933 286-287 -RRB- denotes )
T5934 287-289 , denotes ,
T5935 289-290 DT denotes a
T5937 291-299 JJ denotes chimeric
T5936 300-306 NN denotes intron
T5938 307-311 IN denotes from
T5939 312-315 NN denotes pCI
T5940 316-317 -LRB- denotes (
T5941 317-324 NNP denotes Promega
T5942 324-325 -RRB- denotes )
T5943 326-328 TO denotes to
T5944 329-337 VB denotes increase
T5945 338-345 NN denotes message
T5946 346-355 NN denotes stability
T5947 356-359 CC denotes and
T5948 360-370 NN denotes expression
T5949 371-372 -LRB- denotes [
T5951 372-374 CD denotes 26
T5952 374-375 , denotes ,
T5950 375-377 CD denotes 27
T5953 377-378 -RRB- denotes ]
T5954 378-380 , denotes ,
T5955 380-381 DT denotes a
T5957 382-387 NN denotes Kozak
T5958 388-397 NN denotes consensus
T5956 398-406 NN denotes sequence
T5959 407-409 IN denotes at
T5960 410-413 DT denotes the
T5962 414-424 NN denotes initiation
T5961 425-430 NN denotes codon
T5963 431-433 TO denotes to
T5964 434-442 VB denotes optimize
T5965 443-454 NN denotes translation
T5966 455-456 -LRB- denotes [
T5967 456-458 CD denotes 28
T5968 458-459 -RRB- denotes ]
T5969 459-461 , denotes ,
T5970 461-464 DT denotes the
T5972 465-470 JJ denotes human
T5973 471-473 NN denotes E2
T5971 474-478 NN denotes cDNA
T5974 479-484 WDT denotes which
T5976 485-488 VBZ denotes has
T5977 489-493 VBN denotes been
T5975 494-502 VBN denotes modified
T5978 503-505 TO denotes to
T5979 506-513 VB denotes contain
T5980 514-515 DT denotes a
T5982 516-517 CD denotes 4
T5983 517-518 SYM denotes ×
T5984 519-526 NN denotes alanine
T5981 527-533 NN denotes linker
T5985 534-542 VBN denotes followed
T5986 543-545 IN denotes by
T5987 546-547 DT denotes a
T5989 548-549 NN denotes c
T5991 549-550 HYPH denotes -
T5990 550-553 NN denotes myc
T5992 554-561 NN denotes epitope
T5988 562-565 NN denotes tag
T5993 566-568 IN denotes at
T5994 569-572 DT denotes the
T5996 573-580 NN denotes carboxy
T5995 581-589 NN denotes terminus
T5997 590-592 TO denotes to
T5998 593-603 VB denotes facilitate
T5999 604-613 NN denotes detection
T6000 613-615 , denotes ,
T6001 615-618 CC denotes and
T6002 619-621 DT denotes an
T6004 622-626 NN denotes SV40
T6005 627-631 JJ denotes late
T6006 632-647 NN denotes polyadenylation
T6003 648-656 NN denotes sequence
T6007 657-661 IN denotes from
T6008 662-665 NN denotes pCI
T6009 666-669 IN denotes for
T6010 670-678 VBN denotes enhanced
T6012 679-683 NN denotes mRNA
T6011 684-693 NN denotes stability
T6013 694-697 CC denotes and
T6014 698-709 NN denotes translation
T6015 709-710 . denotes .
R1606 T5877 T5878 amod Standard,techniques
R1607 T5878 T5880 nsubjpass techniques,used
R1608 T5879 T5878 amod molecular,techniques
R1609 T5881 T5880 auxpass were,used
R1610 T5882 T5883 aux to,assemble
R1611 T5883 T5880 advcl assemble,used
R1612 T5884 T5885 det the,construct
R1613 T5885 T5883 dobj construct,assemble
R1614 T5886 T5885 amod transgenic,construct
R1615 T5887 T5885 punct ", ",construct
R1616 T5888 T5889 compound pTRE,E2
R1617 T5889 T5885 appos E2,construct
R1618 T5890 T5889 punct -,E2
R1619 T5891 T5880 punct .,used
R1620 T5893 T5894 det This,transgene
R1621 T5894 T5895 nsubj transgene,contains
R1622 T5896 T5897 det the,promoter
R1623 T5897 T5895 dobj promoter,contains
R1624 T5898 T5897 nmod tetracycline,promoter
R1625 T5899 T5897 amod responsive,promoter
R1626 T5900 T5897 nmod hCMV,promoter
R1627 T5901 T5900 punct *,hCMV
R1628 T5902 T5900 punct -,hCMV
R1629 T5903 T5900 nummod 1,hCMV
R1630 T5904 T5897 punct [,promoter
R1631 T5905 T5897 acl consisting,promoter
R1632 T5906 T5905 prep of,consisting
R1633 T5907 T5908 det the,element
R1634 T5908 T5906 pobj element,of
R1635 T5909 T5908 nmod tetracycline,element
R1636 T5910 T5908 amod responsive,element
R1637 T5911 T5908 punct (,element
R1638 T5912 T5908 appos TRE,element
R1639 T5913 T5908 punct ),element
R1640 T5914 T5908 cc and,element
R1641 T5915 T5916 det a,promoter
R1642 T5916 T5908 conj promoter,element
R1643 T5917 T5916 amod minimal,promoter
R1644 T5918 T5916 compound hCMV,promoter
R1645 T5919 T5916 punct ],promoter
R1646 T5920 T5921 punct [,25
R1647 T5921 T5916 parataxis 25,promoter
R1648 T5922 T5921 punct ],25
R1649 T5923 T5916 prep from,promoter
R1650 T5924 T5923 pobj pTRE2,from
R1651 T5925 T5926 punct (,Inc.
R1652 T5926 T5916 parataxis Inc.,promoter
R1653 T5927 T5926 compound Clontech,Inc.
R1654 T5928 T5926 punct ", ",Inc.
R1655 T5929 T5930 compound Mt.,View
R1656 T5930 T5926 npadvmod View,Inc.
R1657 T5931 T5926 punct ", ",Inc.
R1658 T5932 T5926 npadvmod CA,Inc.
R1659 T5933 T5926 punct ),Inc.
R1660 T5934 T5897 punct ", ",promoter
R1661 T5935 T5936 det a,intron
R1662 T5936 T5897 conj intron,promoter
R1663 T5937 T5936 amod chimeric,intron
R1664 T5938 T5936 prep from,intron
R1665 T5939 T5938 pobj pCI,from
R1666 T5940 T5941 punct (,Promega
R1667 T5941 T5936 parataxis Promega,intron
R1668 T5942 T5941 punct ),Promega
R1669 T5943 T5944 aux to,increase
R1670 T5944 T5936 advcl increase,intron
R1671 T5945 T5946 compound message,stability
R1672 T5946 T5944 dobj stability,increase
R1673 T5947 T5946 cc and,stability
R1674 T5948 T5946 conj expression,stability
R1675 T5949 T5950 punct [,27
R1676 T5950 T5944 parataxis 27,increase
R1677 T5951 T5950 nummod 26,27
R1678 T5952 T5950 punct ",",27
R1679 T5953 T5950 punct ],27
R1680 T5954 T5936 punct ", ",intron
R1681 T5955 T5956 det a,sequence
R1682 T5956 T5936 conj sequence,intron
R1683 T5957 T5956 compound Kozak,sequence
R1684 T5958 T5956 compound consensus,sequence
R1685 T5959 T5956 prep at,sequence
R1686 T5960 T5961 det the,codon
R1687 T5961 T5959 pobj codon,at
R1688 T5962 T5961 compound initiation,codon
R1689 T5963 T5964 aux to,optimize
R1690 T5964 T5956 advcl optimize,sequence
R1691 T5965 T5964 dobj translation,optimize
R1692 T5966 T5967 punct [,28
R1693 T5967 T5964 parataxis 28,optimize
R1694 T5968 T5967 punct ],28
R1695 T5969 T5956 punct ", ",sequence
R1696 T5970 T5971 det the,cDNA
R1697 T5971 T5956 conj cDNA,sequence
R1698 T5972 T5971 amod human,cDNA
R1699 T5973 T5971 compound E2,cDNA
R1700 T5974 T5975 dep which,modified
R1701 T5975 T5971 relcl modified,cDNA
R1702 T5976 T5975 aux has,modified
R1703 T5977 T5975 auxpass been,modified
R1704 T5978 T5979 aux to,contain
R1705 T5979 T5975 advcl contain,modified
R1706 T5980 T5981 det a,linker
R1707 T5981 T5979 dobj linker,contain
R1708 T5982 T5981 nummod 4,linker
R1709 T5983 T5982 punct ×,4
R1710 T5984 T5981 compound alanine,linker
R1711 T5985 T5981 acl followed,linker
R1712 T5986 T5985 agent by,followed
R1713 T5987 T5988 det a,tag
R1714 T5988 T5986 pobj tag,by
R1715 T5989 T5990 compound c,myc
R1716 T5990 T5988 compound myc,tag
R1717 T5991 T5990 punct -,myc
R1718 T5992 T5988 compound epitope,tag
R1719 T5993 T5988 prep at,tag
R1720 T5994 T5995 det the,terminus
R1721 T5995 T5993 pobj terminus,at
R1722 T5996 T5995 compound carboxy,terminus
R1723 T5997 T5998 aux to,facilitate
R1724 T5998 T5988 advcl facilitate,tag
R1725 T5999 T5998 dobj detection,facilitate
R1726 T6000 T5971 punct ", ",cDNA
R1727 T6001 T5971 cc and,cDNA
R1728 T6002 T6003 det an,sequence
R1729 T6003 T5971 conj sequence,cDNA
R1730 T6004 T6003 nmod SV40,sequence
R1731 T6005 T6003 amod late,sequence
R1732 T6006 T6003 compound polyadenylation,sequence
R1733 T6007 T6003 prep from,sequence
R1734 T6008 T6007 pobj pCI,from
R1735 T6009 T6003 prep for,sequence
R1736 T6010 T6011 amod enhanced,stability
R1737 T6011 T6009 pobj stability,for
R1738 T6012 T6011 compound mRNA,stability
R1739 T6013 T6011 cc and,stability
R1740 T6014 T6011 conj translation,stability
R1741 T6015 T5895 punct .,contains