| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T12292 |
0-20 |
NN |
denotes |
Immunohistochemistry |
| T12293 |
20-22 |
, |
denotes |
, |
| T12294 |
22-24 |
FW |
denotes |
in |
| T12295 |
25-29 |
FW |
denotes |
situ |
| T12296 |
30-43 |
NN |
denotes |
hybridisation |
| T12297 |
43-45 |
, |
denotes |
, |
| T12298 |
45-48 |
CC |
denotes |
and |
| T12299 |
49-54 |
NN |
denotes |
TUNEL |
| T12300 |
55-60 |
NN |
denotes |
assay |
| T12301 |
60-61 |
. |
denotes |
. |
| T12302 |
61-185 |
sentence |
denotes |
7.5 dpc decidual swellings were dissected away from maternal tissue and fixed in 4% paraformaldehyde/PBS overnight at 4 °C. |
| T12303 |
62-65 |
CD |
denotes |
7.5 |
| T12304 |
66-69 |
NN |
denotes |
dpc |
| T12306 |
70-78 |
JJ |
denotes |
decidual |
| T12305 |
79-88 |
NNS |
denotes |
swellings |
| T12308 |
89-93 |
VBD |
denotes |
were |
| T12307 |
94-103 |
VBN |
denotes |
dissected |
| T12309 |
104-108 |
RB |
denotes |
away |
| T12310 |
109-113 |
IN |
denotes |
from |
| T12311 |
114-122 |
JJ |
denotes |
maternal |
| T12312 |
123-129 |
NN |
denotes |
tissue |
| T12313 |
130-133 |
CC |
denotes |
and |
| T12314 |
134-139 |
VBN |
denotes |
fixed |
| T12315 |
140-142 |
IN |
denotes |
in |
| T12316 |
143-144 |
CD |
denotes |
4 |
| T12317 |
144-145 |
NN |
denotes |
% |
| T12318 |
146-162 |
NN |
denotes |
paraformaldehyde |
| T12319 |
162-163 |
HYPH |
denotes |
/ |
| T12320 |
163-166 |
NN |
denotes |
PBS |
| T12321 |
167-176 |
RB |
denotes |
overnight |
| T12322 |
177-179 |
IN |
denotes |
at |
| T12323 |
180-181 |
CD |
denotes |
4 |
| T12324 |
182-184 |
NN |
denotes |
°C |
| T12325 |
184-185 |
. |
denotes |
. |
| T12326 |
185-334 |
sentence |
denotes |
After embryos were washed thoroughly in PBS, they were dehydrated through an ethanol series and xylene, embedded in paraffin, and sectioned at 5 μm. |
| T12327 |
186-191 |
IN |
denotes |
After |
| T12329 |
192-199 |
NNS |
denotes |
embryos |
| T12330 |
200-204 |
VBD |
denotes |
were |
| T12328 |
205-211 |
VBN |
denotes |
washed |
| T12332 |
212-222 |
RB |
denotes |
thoroughly |
| T12333 |
223-225 |
IN |
denotes |
in |
| T12334 |
226-229 |
NN |
denotes |
PBS |
| T12335 |
229-231 |
, |
denotes |
, |
| T12336 |
231-235 |
PRP |
denotes |
they |
| T12337 |
236-240 |
VBD |
denotes |
were |
| T12331 |
241-251 |
VBN |
denotes |
dehydrated |
| T12338 |
252-259 |
IN |
denotes |
through |
| T12339 |
260-262 |
DT |
denotes |
an |
| T12341 |
263-270 |
NN |
denotes |
ethanol |
| T12340 |
271-277 |
NNS |
denotes |
series |
| T12342 |
278-281 |
CC |
denotes |
and |
| T12343 |
282-288 |
NN |
denotes |
xylene |
| T12344 |
288-290 |
, |
denotes |
, |
| T12345 |
290-298 |
VBN |
denotes |
embedded |
| T12346 |
299-301 |
IN |
denotes |
in |
| T12347 |
302-310 |
NN |
denotes |
paraffin |
| T12348 |
310-312 |
, |
denotes |
, |
| T12349 |
312-315 |
CC |
denotes |
and |
| T12350 |
316-325 |
VBN |
denotes |
sectioned |
| T12351 |
326-328 |
IN |
denotes |
at |
| T12352 |
329-330 |
CD |
denotes |
5 |
| T12353 |
331-333 |
NNS |
denotes |
μm |
| T12354 |
333-334 |
. |
denotes |
. |
| T12355 |
334-525 |
sentence |
denotes |
Sections were processed for immunohistochemistry using the ABC Staining System (Santa Cruz Biotechnology, Santa Cruz, California, United States) according to the manufacturer's instructions. |
| T12356 |
335-343 |
NNS |
denotes |
Sections |
| T12358 |
344-348 |
VBD |
denotes |
were |
| T12357 |
349-358 |
VBN |
denotes |
processed |
| T12359 |
359-362 |
IN |
denotes |
for |
| T12360 |
363-383 |
NN |
denotes |
immunohistochemistry |
| T12361 |
384-389 |
VBG |
denotes |
using |
| T12362 |
390-393 |
DT |
denotes |
the |
| T12364 |
394-397 |
NNP |
denotes |
ABC |
| T12365 |
398-406 |
NNP |
denotes |
Staining |
| T12363 |
407-413 |
NNP |
denotes |
System |
| T12366 |
414-415 |
-LRB- |
denotes |
( |
| T12368 |
415-420 |
NNP |
denotes |
Santa |
| T12369 |
421-425 |
NNP |
denotes |
Cruz |
| T12367 |
426-439 |
NNP |
denotes |
Biotechnology |
| T12370 |
439-441 |
, |
denotes |
, |
| T12371 |
441-446 |
NNP |
denotes |
Santa |
| T12372 |
447-451 |
NNP |
denotes |
Cruz |
| T12373 |
451-453 |
, |
denotes |
, |
| T12374 |
453-463 |
NNP |
denotes |
California |
| T12375 |
463-465 |
, |
denotes |
, |
| T12376 |
465-471 |
NNP |
denotes |
United |
| T12377 |
472-478 |
NNP |
denotes |
States |
| T12378 |
478-479 |
-RRB- |
denotes |
) |
| T12379 |
480-489 |
VBG |
denotes |
according |
| T12380 |
490-492 |
IN |
denotes |
to |
| T12381 |
493-496 |
DT |
denotes |
the |
| T12382 |
497-509 |
NN |
denotes |
manufacturer |
| T12384 |
509-511 |
POS |
denotes |
's |
| T12383 |
512-524 |
NNS |
denotes |
instructions |
| T12385 |
524-525 |
. |
denotes |
. |
| T12386 |
525-823 |
sentence |
denotes |
Sections were stained with rabbit polyclonal antibodies against ATRX (H-300, Santa Cruz Biotechnology), Placental lactogen-I (AB1288, Chemicon International, Temecula, California, United States) and phospho (Ser10)-histone H3 (06–570, Upstate Biotechnology, Waltham, Massachusetts, United States). |
| T12387 |
526-534 |
NNS |
denotes |
Sections |
| T12389 |
535-539 |
VBD |
denotes |
were |
| T12388 |
540-547 |
VBN |
denotes |
stained |
| T12390 |
548-552 |
IN |
denotes |
with |
| T12391 |
553-559 |
NN |
denotes |
rabbit |
| T12393 |
560-570 |
JJ |
denotes |
polyclonal |
| T12392 |
571-581 |
NNS |
denotes |
antibodies |
| T12394 |
582-589 |
IN |
denotes |
against |
| T12395 |
590-594 |
NN |
denotes |
ATRX |
| T12396 |
595-596 |
-LRB- |
denotes |
( |
| T12397 |
596-597 |
NN |
denotes |
H |
| T12398 |
597-598 |
HYPH |
denotes |
- |
| T12399 |
598-601 |
CD |
denotes |
300 |
| T12400 |
601-603 |
, |
denotes |
, |
| T12401 |
603-608 |
NNP |
denotes |
Santa |
| T12402 |
609-613 |
NNP |
denotes |
Cruz |
| T12403 |
614-627 |
NNP |
denotes |
Biotechnology |
| T12404 |
627-628 |
-RRB- |
denotes |
) |
| T12405 |
628-630 |
, |
denotes |
, |
| T12406 |
630-639 |
JJ |
denotes |
Placental |
| T12408 |
640-648 |
NN |
denotes |
lactogen |
| T12409 |
648-649 |
HYPH |
denotes |
- |
| T12407 |
649-650 |
NN |
denotes |
I |
| T12410 |
651-652 |
-LRB- |
denotes |
( |
| T12411 |
652-658 |
NN |
denotes |
AB1288 |
| T12412 |
658-660 |
, |
denotes |
, |
| T12413 |
660-668 |
NNP |
denotes |
Chemicon |
| T12414 |
669-682 |
NNP |
denotes |
International |
| T12415 |
682-684 |
, |
denotes |
, |
| T12416 |
684-692 |
NNP |
denotes |
Temecula |
| T12417 |
692-694 |
, |
denotes |
, |
| T12418 |
694-704 |
NNP |
denotes |
California |
| T12419 |
704-706 |
, |
denotes |
, |
| T12420 |
706-712 |
NNP |
denotes |
United |
| T12421 |
713-719 |
NNP |
denotes |
States |
| T12422 |
719-720 |
-RRB- |
denotes |
) |
| T12423 |
721-724 |
CC |
denotes |
and |
| T12424 |
725-732 |
NN |
denotes |
phospho |
| T12426 |
733-734 |
-LRB- |
denotes |
( |
| T12427 |
734-739 |
NN |
denotes |
Ser10 |
| T12428 |
739-740 |
-RRB- |
denotes |
) |
| T12429 |
740-741 |
HYPH |
denotes |
- |
| T12425 |
741-748 |
NN |
denotes |
histone |
| T12430 |
749-751 |
NN |
denotes |
H3 |
| T12431 |
752-753 |
-LRB- |
denotes |
( |
| T12433 |
753-755 |
CD |
denotes |
06 |
| T12434 |
755-756 |
HYPH |
denotes |
– |
| T12432 |
756-759 |
CD |
denotes |
570 |
| T12435 |
759-761 |
, |
denotes |
, |
| T12436 |
761-768 |
NNP |
denotes |
Upstate |
| T12437 |
769-782 |
NNP |
denotes |
Biotechnology |
| T12438 |
782-784 |
, |
denotes |
, |
| T12439 |
784-791 |
NNP |
denotes |
Waltham |
| T12440 |
791-793 |
, |
denotes |
, |
| T12441 |
793-806 |
NNP |
denotes |
Massachusetts |
| T12442 |
806-808 |
, |
denotes |
, |
| T12443 |
808-814 |
NNP |
denotes |
United |
| T12444 |
815-821 |
NNP |
denotes |
States |
| T12445 |
821-822 |
-RRB- |
denotes |
) |
| T12446 |
822-823 |
. |
denotes |
. |
| T12447 |
823-892 |
sentence |
denotes |
Where appropriate, adjacent sections were stained with haematoxylin. |
| T12448 |
824-829 |
WRB |
denotes |
Where |
| T12449 |
830-841 |
JJ |
denotes |
appropriate |
| T12451 |
841-843 |
, |
denotes |
, |
| T12452 |
843-851 |
JJ |
denotes |
adjacent |
| T12453 |
852-860 |
NNS |
denotes |
sections |
| T12454 |
861-865 |
VBD |
denotes |
were |
| T12450 |
866-873 |
VBN |
denotes |
stained |
| T12455 |
874-878 |
IN |
denotes |
with |
| T12456 |
879-891 |
NN |
denotes |
haematoxylin |
| T12457 |
891-892 |
. |
denotes |
. |
| T12458 |
892-1051 |
sentence |
denotes |
In some cases, adjacent sections were also analysed to detect apoptotic cells by TUNEL using the in situ cell death detection kit (Roche, Basel, Switzerland). |
| T12459 |
893-895 |
IN |
denotes |
In |
| T12461 |
896-900 |
DT |
denotes |
some |
| T12462 |
901-906 |
NNS |
denotes |
cases |
| T12463 |
906-908 |
, |
denotes |
, |
| T12464 |
908-916 |
JJ |
denotes |
adjacent |
| T12465 |
917-925 |
NNS |
denotes |
sections |
| T12466 |
926-930 |
VBD |
denotes |
were |
| T12467 |
931-935 |
RB |
denotes |
also |
| T12460 |
936-944 |
VBN |
denotes |
analysed |
| T12468 |
945-947 |
TO |
denotes |
to |
| T12469 |
948-954 |
VB |
denotes |
detect |
| T12470 |
955-964 |
JJ |
denotes |
apoptotic |
| T12471 |
965-970 |
NNS |
denotes |
cells |
| T12472 |
971-973 |
IN |
denotes |
by |
| T12473 |
974-979 |
NN |
denotes |
TUNEL |
| T12474 |
980-985 |
VBG |
denotes |
using |
| T12475 |
986-989 |
DT |
denotes |
the |
| T12477 |
990-992 |
FW |
denotes |
in |
| T12478 |
993-997 |
FW |
denotes |
situ |
| T12479 |
998-1002 |
NN |
denotes |
cell |
| T12480 |
1003-1008 |
NN |
denotes |
death |
| T12481 |
1009-1018 |
NN |
denotes |
detection |
| T12476 |
1019-1022 |
NN |
denotes |
kit |
| T12482 |
1023-1024 |
-LRB- |
denotes |
( |
| T12483 |
1024-1029 |
NNP |
denotes |
Roche |
| T12484 |
1029-1031 |
, |
denotes |
, |
| T12485 |
1031-1036 |
NNP |
denotes |
Basel |
| T12486 |
1036-1038 |
, |
denotes |
, |
| T12487 |
1038-1049 |
NNP |
denotes |
Switzerland |
| T12488 |
1049-1050 |
-RRB- |
denotes |
) |
| T12489 |
1050-1051 |
. |
denotes |
. |
| T12490 |
1051-1229 |
sentence |
denotes |
After labelling, these slides were mounted in Vectashield containing DAPI (Vector Laboratories, Burlingame, California, United States) and visualised by fluorescence microscopy. |
| T12491 |
1052-1057 |
IN |
denotes |
After |
| T12493 |
1058-1067 |
NN |
denotes |
labelling |
| T12494 |
1067-1069 |
, |
denotes |
, |
| T12495 |
1069-1074 |
DT |
denotes |
these |
| T12496 |
1075-1081 |
NNS |
denotes |
slides |
| T12497 |
1082-1086 |
VBD |
denotes |
were |
| T12492 |
1087-1094 |
VBN |
denotes |
mounted |
| T12498 |
1095-1097 |
IN |
denotes |
in |
| T12499 |
1098-1109 |
NNP |
denotes |
Vectashield |
| T12500 |
1110-1120 |
VBG |
denotes |
containing |
| T12501 |
1121-1125 |
NN |
denotes |
DAPI |
| T12502 |
1126-1127 |
-LRB- |
denotes |
( |
| T12504 |
1127-1133 |
NNP |
denotes |
Vector |
| T12503 |
1134-1146 |
NNP |
denotes |
Laboratories |
| T12505 |
1146-1148 |
, |
denotes |
, |
| T12506 |
1148-1158 |
NNP |
denotes |
Burlingame |
| T12507 |
1158-1160 |
, |
denotes |
, |
| T12508 |
1160-1170 |
NNP |
denotes |
California |
| T12509 |
1170-1172 |
, |
denotes |
, |
| T12510 |
1172-1178 |
NNP |
denotes |
United |
| T12511 |
1179-1185 |
NNP |
denotes |
States |
| T12512 |
1185-1186 |
-RRB- |
denotes |
) |
| T12513 |
1187-1190 |
CC |
denotes |
and |
| T12514 |
1191-1201 |
VBN |
denotes |
visualised |
| T12515 |
1202-1204 |
IN |
denotes |
by |
| T12516 |
1205-1217 |
NN |
denotes |
fluorescence |
| T12517 |
1218-1228 |
NN |
denotes |
microscopy |
| T12518 |
1228-1229 |
. |
denotes |
. |
| T12519 |
1229-1560 |
sentence |
denotes |
Whole-mount in situ hybridisations were performed on 8.5 dpc embryos (dissected away from maternal and extraembryonic tissues) using a brachyury (T) riboprobe [16] and on bisected decidual implantation sites from which embryos (8.5 dpc) had been removed using a placental lactogen-1 (Pl-1) riboprobe (see Protocol S1 for details). |
| T12520 |
1230-1235 |
JJ |
denotes |
Whole |
| T12522 |
1235-1236 |
HYPH |
denotes |
- |
| T12521 |
1236-1241 |
NN |
denotes |
mount |
| T12524 |
1242-1244 |
FW |
denotes |
in |
| T12525 |
1245-1249 |
FW |
denotes |
situ |
| T12523 |
1250-1264 |
NNS |
denotes |
hybridisations |
| T12527 |
1265-1269 |
VBD |
denotes |
were |
| T12526 |
1270-1279 |
VBN |
denotes |
performed |
| T12528 |
1280-1282 |
IN |
denotes |
on |
| T12529 |
1283-1286 |
CD |
denotes |
8.5 |
| T12530 |
1287-1290 |
NN |
denotes |
dpc |
| T12531 |
1291-1298 |
NNS |
denotes |
embryos |
| T12532 |
1299-1300 |
-LRB- |
denotes |
( |
| T12533 |
1300-1309 |
VBN |
denotes |
dissected |
| T12534 |
1310-1314 |
RB |
denotes |
away |
| T12535 |
1315-1319 |
IN |
denotes |
from |
| T12536 |
1320-1328 |
JJ |
denotes |
maternal |
| T12538 |
1329-1332 |
CC |
denotes |
and |
| T12539 |
1333-1347 |
JJ |
denotes |
extraembryonic |
| T12537 |
1348-1355 |
NNS |
denotes |
tissues |
| T12540 |
1355-1356 |
-RRB- |
denotes |
) |
| T12541 |
1357-1362 |
VBG |
denotes |
using |
| T12542 |
1363-1364 |
DT |
denotes |
a |
| T12544 |
1365-1374 |
NN |
denotes |
brachyury |
| T12545 |
1375-1376 |
-LRB- |
denotes |
( |
| T12546 |
1376-1377 |
NN |
denotes |
T |
| T12547 |
1377-1378 |
-RRB- |
denotes |
) |
| T12543 |
1379-1388 |
NN |
denotes |
riboprobe |
| T12548 |
1389-1390 |
-LRB- |
denotes |
[ |
| T12549 |
1390-1392 |
CD |
denotes |
16 |
| T12550 |
1392-1393 |
-RRB- |
denotes |
] |
| T12551 |
1394-1397 |
CC |
denotes |
and |
| T12552 |
1398-1400 |
IN |
denotes |
on |
| T12553 |
1401-1409 |
VBN |
denotes |
bisected |
| T12555 |
1410-1418 |
JJ |
denotes |
decidual |
| T12556 |
1419-1431 |
NN |
denotes |
implantation |
| T12554 |
1432-1437 |
NNS |
denotes |
sites |
| T12557 |
1438-1442 |
IN |
denotes |
from |
| T12559 |
1443-1448 |
WDT |
denotes |
which |
| T12560 |
1449-1456 |
NNS |
denotes |
embryos |
| T12561 |
1457-1458 |
-LRB- |
denotes |
( |
| T12563 |
1458-1461 |
CD |
denotes |
8.5 |
| T12562 |
1462-1465 |
NN |
denotes |
dpc |
| T12564 |
1465-1466 |
-RRB- |
denotes |
) |
| T12565 |
1467-1470 |
VBD |
denotes |
had |
| T12566 |
1471-1475 |
VBN |
denotes |
been |
| T12558 |
1476-1483 |
VBN |
denotes |
removed |
| T12567 |
1484-1489 |
VBG |
denotes |
using |
| T12568 |
1490-1491 |
DT |
denotes |
a |
| T12570 |
1492-1501 |
JJ |
denotes |
placental |
| T12571 |
1502-1510 |
NN |
denotes |
lactogen |
| T12572 |
1510-1511 |
HYPH |
denotes |
- |
| T12573 |
1511-1512 |
CD |
denotes |
1 |
| T12574 |
1513-1514 |
-LRB- |
denotes |
( |
| T12575 |
1514-1516 |
NN |
denotes |
Pl |
| T12576 |
1516-1517 |
HYPH |
denotes |
- |
| T12577 |
1517-1518 |
CD |
denotes |
1 |
| T12578 |
1518-1519 |
-RRB- |
denotes |
) |
| T12569 |
1520-1529 |
NN |
denotes |
riboprobe |
| T12579 |
1530-1531 |
-LRB- |
denotes |
( |
| T12580 |
1531-1534 |
VB |
denotes |
see |
| T12581 |
1535-1543 |
NN |
denotes |
Protocol |
| T12582 |
1544-1546 |
NN |
denotes |
S1 |
| T12583 |
1547-1550 |
IN |
denotes |
for |
| T12584 |
1551-1558 |
NNS |
denotes |
details |
| T12585 |
1558-1559 |
-RRB- |
denotes |
) |
| T12586 |
1559-1560 |
. |
denotes |
. |
| R7662 |
T12293 |
T12292 |
punct |
", ",Immunohistochemistry |
| R7663 |
T12294 |
T12295 |
nmod |
in,situ |
| R7664 |
T12295 |
T12296 |
nmod |
situ,hybridisation |
| R7665 |
T12296 |
T12292 |
conj |
hybridisation,Immunohistochemistry |
| R7666 |
T12297 |
T12296 |
punct |
", ",hybridisation |
| R7667 |
T12298 |
T12296 |
cc |
and,hybridisation |
| R7668 |
T12299 |
T12300 |
compound |
TUNEL,assay |
| R7669 |
T12300 |
T12296 |
conj |
assay,hybridisation |
| R7670 |
T12301 |
T12300 |
punct |
.,assay |
| R7671 |
T12303 |
T12304 |
nummod |
7.5,dpc |
| R7672 |
T12304 |
T12305 |
nmod |
dpc,swellings |
| R7673 |
T12305 |
T12307 |
nsubjpass |
swellings,dissected |
| R7674 |
T12306 |
T12305 |
amod |
decidual,swellings |
| R7675 |
T12308 |
T12307 |
auxpass |
were,dissected |
| R7676 |
T12309 |
T12307 |
advmod |
away,dissected |
| R7677 |
T12310 |
T12309 |
prep |
from,away |
| R7678 |
T12311 |
T12312 |
amod |
maternal,tissue |
| R7679 |
T12312 |
T12310 |
pobj |
tissue,from |
| R7680 |
T12313 |
T12307 |
cc |
and,dissected |
| R7681 |
T12314 |
T12307 |
conj |
fixed,dissected |
| R7682 |
T12315 |
T12314 |
prep |
in,fixed |
| R7683 |
T12316 |
T12317 |
nummod |
4,% |
| R7684 |
T12317 |
T12318 |
compound |
%,paraformaldehyde |
| R7685 |
T12318 |
T12315 |
pobj |
paraformaldehyde,in |
| R7686 |
T12319 |
T12318 |
punct |
/,paraformaldehyde |
| R7687 |
T12320 |
T12318 |
appos |
PBS,paraformaldehyde |
| R7688 |
T12321 |
T12314 |
advmod |
overnight,fixed |
| R7689 |
T12322 |
T12314 |
prep |
at,fixed |
| R7690 |
T12323 |
T12324 |
nummod |
4,°C |
| R7691 |
T12324 |
T12322 |
pobj |
°C,at |
| R7692 |
T12325 |
T12307 |
punct |
.,dissected |
| R7693 |
T12327 |
T12328 |
mark |
After,washed |
| R7694 |
T12328 |
T12331 |
advcl |
washed,dehydrated |
| R7695 |
T12329 |
T12328 |
nsubjpass |
embryos,washed |
| R7696 |
T12330 |
T12328 |
auxpass |
were,washed |
| R7697 |
T12332 |
T12328 |
advmod |
thoroughly,washed |
| R7698 |
T12333 |
T12328 |
prep |
in,washed |
| R7699 |
T12334 |
T12333 |
pobj |
PBS,in |
| R7700 |
T12335 |
T12331 |
punct |
", ",dehydrated |
| R7701 |
T12336 |
T12331 |
nsubjpass |
they,dehydrated |
| R7702 |
T12337 |
T12331 |
auxpass |
were,dehydrated |
| R7703 |
T12338 |
T12331 |
prep |
through,dehydrated |
| R7704 |
T12339 |
T12340 |
det |
an,series |
| R7705 |
T12340 |
T12338 |
pobj |
series,through |
| R7706 |
T12341 |
T12340 |
compound |
ethanol,series |
| R7707 |
T12342 |
T12340 |
cc |
and,series |
| R7708 |
T12343 |
T12340 |
conj |
xylene,series |
| R7709 |
T12344 |
T12331 |
punct |
", ",dehydrated |
| R7710 |
T12345 |
T12331 |
conj |
embedded,dehydrated |
| R7711 |
T12346 |
T12345 |
prep |
in,embedded |
| R7712 |
T12347 |
T12346 |
pobj |
paraffin,in |
| R7713 |
T12348 |
T12345 |
punct |
", ",embedded |
| R7714 |
T12349 |
T12345 |
cc |
and,embedded |
| R7715 |
T12350 |
T12345 |
conj |
sectioned,embedded |
| R7716 |
T12351 |
T12350 |
prep |
at,sectioned |
| R7717 |
T12352 |
T12353 |
nummod |
5,μm |
| R7718 |
T12353 |
T12351 |
pobj |
μm,at |
| R7719 |
T12354 |
T12331 |
punct |
.,dehydrated |
| R7720 |
T12356 |
T12357 |
nsubjpass |
Sections,processed |
| R7721 |
T12358 |
T12357 |
auxpass |
were,processed |
| R7722 |
T12359 |
T12357 |
prep |
for,processed |
| R7723 |
T12360 |
T12359 |
pobj |
immunohistochemistry,for |
| R7724 |
T12361 |
T12357 |
advcl |
using,processed |
| R7725 |
T12362 |
T12363 |
det |
the,System |
| R7726 |
T12363 |
T12361 |
dobj |
System,using |
| R7727 |
T12364 |
T12363 |
compound |
ABC,System |
| R7728 |
T12365 |
T12363 |
compound |
Staining,System |
| R7729 |
T12366 |
T12367 |
punct |
(,Biotechnology |
| R7730 |
T12367 |
T12363 |
parataxis |
Biotechnology,System |
| R7731 |
T12368 |
T12369 |
compound |
Santa,Cruz |
| R7732 |
T12369 |
T12367 |
compound |
Cruz,Biotechnology |
| R7733 |
T12370 |
T12367 |
punct |
", ",Biotechnology |
| R7734 |
T12371 |
T12372 |
compound |
Santa,Cruz |
| R7735 |
T12372 |
T12367 |
npadvmod |
Cruz,Biotechnology |
| R7736 |
T12373 |
T12367 |
punct |
", ",Biotechnology |
| R7737 |
T12374 |
T12367 |
npadvmod |
California,Biotechnology |
| R7738 |
T12375 |
T12367 |
punct |
", ",Biotechnology |
| R7739 |
T12376 |
T12377 |
compound |
United,States |
| R7740 |
T12377 |
T12367 |
npadvmod |
States,Biotechnology |
| R7741 |
T12378 |
T12367 |
punct |
),Biotechnology |
| R7742 |
T12379 |
T12361 |
prep |
according,using |
| R7743 |
T12380 |
T12379 |
prep |
to,according |
| R7744 |
T12381 |
T12382 |
det |
the,manufacturer |
| R7745 |
T12382 |
T12383 |
poss |
manufacturer,instructions |
| R7746 |
T12383 |
T12380 |
pobj |
instructions,to |
| R7747 |
T12384 |
T12382 |
case |
's,manufacturer |
| R7748 |
T12385 |
T12357 |
punct |
.,processed |
| R7749 |
T12387 |
T12388 |
nsubjpass |
Sections,stained |
| R7750 |
T12389 |
T12388 |
auxpass |
were,stained |
| R7751 |
T12390 |
T12388 |
prep |
with,stained |
| R7752 |
T12391 |
T12392 |
nmod |
rabbit,antibodies |
| R7753 |
T12392 |
T12390 |
pobj |
antibodies,with |
| R7754 |
T12393 |
T12392 |
amod |
polyclonal,antibodies |
| R7755 |
T12394 |
T12392 |
prep |
against,antibodies |
| R7756 |
T12395 |
T12394 |
pobj |
ATRX,against |
| R7757 |
T12396 |
T12397 |
punct |
(,H |
| R7758 |
T12397 |
T12395 |
parataxis |
H,ATRX |
| R7759 |
T12398 |
T12397 |
punct |
-,H |
| R7760 |
T12399 |
T12397 |
nummod |
300,H |
| R7761 |
T12400 |
T12397 |
punct |
", ",H |
| R7762 |
T12401 |
T12402 |
compound |
Santa,Cruz |
| R7763 |
T12402 |
T12403 |
compound |
Cruz,Biotechnology |
| R7764 |
T12403 |
T12397 |
npadvmod |
Biotechnology,H |
| R7765 |
T12404 |
T12397 |
punct |
),H |
| R7766 |
T12405 |
T12395 |
punct |
", ",ATRX |
| R7767 |
T12406 |
T12407 |
amod |
Placental,I |
| R7768 |
T12407 |
T12395 |
conj |
I,ATRX |
| R7769 |
T12408 |
T12407 |
compound |
lactogen,I |
| R7770 |
T12409 |
T12407 |
punct |
-,I |
| R7771 |
T12410 |
T12411 |
punct |
(,AB1288 |
| R7772 |
T12411 |
T12407 |
parataxis |
AB1288,I |
| R7773 |
T12412 |
T12411 |
punct |
", ",AB1288 |
| R7774 |
T12413 |
T12414 |
compound |
Chemicon,International |
| R7775 |
T12414 |
T12411 |
npadvmod |
International,AB1288 |
| R7776 |
T12415 |
T12411 |
punct |
", ",AB1288 |
| R7777 |
T12416 |
T12411 |
npadvmod |
Temecula,AB1288 |
| R7778 |
T12417 |
T12411 |
punct |
", ",AB1288 |
| R7779 |
T12418 |
T12411 |
npadvmod |
California,AB1288 |
| R7780 |
T12419 |
T12411 |
punct |
", ",AB1288 |
| R7781 |
T12420 |
T12421 |
compound |
United,States |
| R7782 |
T12421 |
T12411 |
npadvmod |
States,AB1288 |
| R7783 |
T12422 |
T12411 |
punct |
),AB1288 |
| R7784 |
T12423 |
T12407 |
cc |
and,I |
| R7785 |
T12424 |
T12425 |
nmod |
phospho,histone |
| R7786 |
T12425 |
T12430 |
compound |
histone,H3 |
| R7787 |
T12426 |
T12427 |
punct |
(,Ser10 |
| R7788 |
T12427 |
T12425 |
parataxis |
Ser10,histone |
| R7789 |
T12428 |
T12427 |
punct |
),Ser10 |
| R7790 |
T12429 |
T12425 |
punct |
-,histone |
| R7791 |
T12430 |
T12407 |
conj |
H3,I |
| R7792 |
T12431 |
T12432 |
punct |
(,570 |
| R7793 |
T12432 |
T12430 |
parataxis |
570,H3 |
| R7794 |
T12433 |
T12432 |
nummod |
06,570 |
| R7795 |
T12434 |
T12432 |
punct |
–,570 |
| R7796 |
T12435 |
T12432 |
punct |
", ",570 |
| R7797 |
T12436 |
T12437 |
compound |
Upstate,Biotechnology |
| R7798 |
T12437 |
T12432 |
npadvmod |
Biotechnology,570 |
| R7799 |
T12438 |
T12432 |
punct |
", ",570 |
| R7800 |
T12439 |
T12432 |
npadvmod |
Waltham,570 |
| R7801 |
T12440 |
T12432 |
punct |
", ",570 |
| R7802 |
T12441 |
T12432 |
npadvmod |
Massachusetts,570 |
| R7803 |
T12442 |
T12432 |
punct |
", ",570 |
| R7804 |
T12443 |
T12444 |
compound |
United,States |
| R7805 |
T12444 |
T12432 |
npadvmod |
States,570 |
| R7806 |
T12445 |
T12432 |
punct |
),570 |
| R7807 |
T12446 |
T12388 |
punct |
.,stained |
| R7808 |
T12448 |
T12449 |
advmod |
Where,appropriate |
| R7809 |
T12449 |
T12450 |
advcl |
appropriate,stained |
| R7810 |
T12451 |
T12450 |
punct |
", ",stained |
| R7811 |
T12452 |
T12453 |
amod |
adjacent,sections |
| R7812 |
T12453 |
T12450 |
nsubjpass |
sections,stained |
| R7813 |
T12454 |
T12450 |
auxpass |
were,stained |
| R7814 |
T12455 |
T12450 |
prep |
with,stained |
| R7815 |
T12456 |
T12455 |
pobj |
haematoxylin,with |
| R7816 |
T12457 |
T12450 |
punct |
.,stained |
| R7817 |
T12459 |
T12460 |
prep |
In,analysed |
| R7818 |
T12461 |
T12462 |
det |
some,cases |
| R7819 |
T12462 |
T12459 |
pobj |
cases,In |
| R7820 |
T12463 |
T12460 |
punct |
", ",analysed |
| R7821 |
T12464 |
T12465 |
amod |
adjacent,sections |
| R7822 |
T12465 |
T12460 |
nsubjpass |
sections,analysed |
| R7823 |
T12466 |
T12460 |
auxpass |
were,analysed |
| R7824 |
T12467 |
T12460 |
advmod |
also,analysed |
| R7825 |
T12468 |
T12469 |
aux |
to,detect |
| R7826 |
T12469 |
T12460 |
advcl |
detect,analysed |
| R7827 |
T12470 |
T12471 |
amod |
apoptotic,cells |
| R7828 |
T12471 |
T12469 |
dobj |
cells,detect |
| R7829 |
T12472 |
T12469 |
prep |
by,detect |
| R7830 |
T12473 |
T12472 |
pobj |
TUNEL,by |
| R7831 |
T12474 |
T12469 |
advcl |
using,detect |
| R7832 |
T12475 |
T12476 |
det |
the,kit |
| R7833 |
T12476 |
T12474 |
dobj |
kit,using |
| R7834 |
T12477 |
T12478 |
advmod |
in,situ |
| R7835 |
T12478 |
T12476 |
amod |
situ,kit |
| R7836 |
T12479 |
T12480 |
compound |
cell,death |
| R7837 |
T12480 |
T12481 |
compound |
death,detection |
| R7838 |
T12481 |
T12476 |
compound |
detection,kit |
| R7839 |
T12482 |
T12483 |
punct |
(,Roche |
| R7840 |
T12483 |
T12474 |
parataxis |
Roche,using |
| R7841 |
T12484 |
T12483 |
punct |
", ",Roche |
| R7842 |
T12485 |
T12483 |
npadvmod |
Basel,Roche |
| R7843 |
T12486 |
T12483 |
punct |
", ",Roche |
| R7844 |
T12487 |
T12483 |
npadvmod |
Switzerland,Roche |
| R7845 |
T12488 |
T12483 |
punct |
),Roche |
| R7846 |
T12489 |
T12460 |
punct |
.,analysed |
| R7847 |
T12491 |
T12492 |
prep |
After,mounted |
| R7848 |
T12493 |
T12491 |
pobj |
labelling,After |
| R7849 |
T12494 |
T12492 |
punct |
", ",mounted |
| R7850 |
T12495 |
T12496 |
det |
these,slides |
| R7851 |
T12496 |
T12492 |
nsubjpass |
slides,mounted |
| R7852 |
T12497 |
T12492 |
auxpass |
were,mounted |
| R7853 |
T12498 |
T12492 |
prep |
in,mounted |
| R7854 |
T12499 |
T12498 |
pobj |
Vectashield,in |
| R7855 |
T12500 |
T12499 |
acl |
containing,Vectashield |
| R7856 |
T12501 |
T12500 |
dobj |
DAPI,containing |
| R7857 |
T12502 |
T12503 |
punct |
(,Laboratories |
| R7858 |
T12503 |
T12499 |
parataxis |
Laboratories,Vectashield |
| R7859 |
T12504 |
T12503 |
compound |
Vector,Laboratories |
| R7860 |
T12505 |
T12503 |
punct |
", ",Laboratories |
| R7861 |
T12506 |
T12503 |
npadvmod |
Burlingame,Laboratories |
| R7862 |
T12507 |
T12503 |
punct |
", ",Laboratories |
| R7863 |
T12508 |
T12503 |
npadvmod |
California,Laboratories |
| R7864 |
T12509 |
T12503 |
punct |
", ",Laboratories |
| R7865 |
T12510 |
T12511 |
compound |
United,States |
| R7866 |
T12511 |
T12503 |
npadvmod |
States,Laboratories |
| R7867 |
T12512 |
T12503 |
punct |
),Laboratories |
| R7868 |
T12513 |
T12492 |
cc |
and,mounted |
| R7869 |
T12514 |
T12492 |
conj |
visualised,mounted |
| R7870 |
T12515 |
T12514 |
prep |
by,visualised |
| R7871 |
T12516 |
T12517 |
compound |
fluorescence,microscopy |
| R7872 |
T12517 |
T12515 |
pobj |
microscopy,by |
| R7873 |
T12518 |
T12492 |
punct |
.,mounted |
| R7874 |
T12520 |
T12521 |
amod |
Whole,mount |
| R7875 |
T12521 |
T12523 |
nmod |
mount,hybridisations |
| R7876 |
T12522 |
T12521 |
punct |
-,mount |
| R7877 |
T12523 |
T12526 |
nsubjpass |
hybridisations,performed |
| R7878 |
T12524 |
T12525 |
advmod |
in,situ |
| R7879 |
T12525 |
T12523 |
amod |
situ,hybridisations |
| R7880 |
T12527 |
T12526 |
auxpass |
were,performed |
| R7881 |
T12528 |
T12526 |
prep |
on,performed |
| R7882 |
T12529 |
T12530 |
nummod |
8.5,dpc |
| R7883 |
T12530 |
T12531 |
compound |
dpc,embryos |
| R7884 |
T12531 |
T12528 |
pobj |
embryos,on |
| R7885 |
T12532 |
T12531 |
punct |
(,embryos |
| R7886 |
T12533 |
T12531 |
acl |
dissected,embryos |
| R7887 |
T12534 |
T12533 |
advmod |
away,dissected |
| R7888 |
T12535 |
T12534 |
prep |
from,away |
| R7889 |
T12536 |
T12537 |
amod |
maternal,tissues |
| R7890 |
T12537 |
T12535 |
pobj |
tissues,from |
| R7891 |
T12538 |
T12536 |
cc |
and,maternal |
| R7892 |
T12539 |
T12536 |
conj |
extraembryonic,maternal |
| R7893 |
T12540 |
T12533 |
punct |
),dissected |
| R7894 |
T12541 |
T12533 |
advcl |
using,dissected |
| R7895 |
T12542 |
T12543 |
det |
a,riboprobe |
| R7896 |
T12543 |
T12541 |
dobj |
riboprobe,using |
| R7897 |
T12544 |
T12543 |
nmod |
brachyury,riboprobe |
| R7898 |
T12545 |
T12544 |
punct |
(,brachyury |
| R7899 |
T12546 |
T12544 |
appos |
T,brachyury |
| R7900 |
T12547 |
T12543 |
punct |
),riboprobe |
| R7901 |
T12548 |
T12549 |
punct |
[,16 |
| R7902 |
T12549 |
T12541 |
parataxis |
16,using |
| R7903 |
T12550 |
T12549 |
punct |
],16 |
| R7904 |
T12551 |
T12528 |
cc |
and,on |
| R7905 |
T12552 |
T12528 |
conj |
on,on |
| R7906 |
T12553 |
T12554 |
amod |
bisected,sites |
| R7907 |
T12554 |
T12552 |
pobj |
sites,on |
| R7908 |
T12555 |
T12554 |
amod |
decidual,sites |
| R7909 |
T12556 |
T12554 |
compound |
implantation,sites |
| R7910 |
T12557 |
T12558 |
prep |
from,removed |
| R7911 |
T12558 |
T12554 |
relcl |
removed,sites |
| R7912 |
T12559 |
T12557 |
pobj |
which,from |
| R7913 |
T12560 |
T12558 |
nsubjpass |
embryos,removed |
| R7914 |
T12561 |
T12562 |
punct |
(,dpc |
| R7915 |
T12562 |
T12560 |
parataxis |
dpc,embryos |
| R7916 |
T12563 |
T12562 |
nummod |
8.5,dpc |
| R7917 |
T12564 |
T12562 |
punct |
),dpc |
| R7918 |
T12565 |
T12558 |
aux |
had,removed |
| R7919 |
T12566 |
T12558 |
auxpass |
been,removed |
| R7920 |
T12567 |
T12558 |
advcl |
using,removed |
| R7921 |
T12568 |
T12569 |
det |
a,riboprobe |
| R7922 |
T12569 |
T12567 |
dobj |
riboprobe,using |
| R7923 |
T12570 |
T12571 |
amod |
placental,lactogen |
| R7924 |
T12571 |
T12569 |
nmod |
lactogen,riboprobe |
| R7925 |
T12572 |
T12571 |
punct |
-,lactogen |
| R7926 |
T12573 |
T12571 |
nummod |
1,lactogen |
| R7927 |
T12574 |
T12571 |
punct |
(,lactogen |
| R7928 |
T12575 |
T12571 |
appos |
Pl,lactogen |
| R7929 |
T12576 |
T12575 |
punct |
-,Pl |
| R7930 |
T12577 |
T12575 |
nummod |
1,Pl |
| R7931 |
T12578 |
T12569 |
punct |
),riboprobe |
| R7932 |
T12579 |
T12580 |
punct |
(,see |
| R7933 |
T12580 |
T12558 |
parataxis |
see,removed |
| R7934 |
T12581 |
T12582 |
compound |
Protocol,S1 |
| R7935 |
T12582 |
T12580 |
dobj |
S1,see |
| R7936 |
T12583 |
T12580 |
prep |
for,see |
| R7937 |
T12584 |
T12583 |
pobj |
details,for |
| R7938 |
T12585 |
T12580 |
punct |
),see |
| R7939 |
T12586 |
T12526 |
punct |
.,performed |
