| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T12092 |
0-8 |
VBN |
denotes |
Targeted |
| T12094 |
9-18 |
NN |
denotes |
Atrx flox |
| T12096 |
19-21 |
NN |
denotes |
ES |
| T12095 |
22-26 |
NN |
denotes |
cell |
| T12093 |
27-33 |
NNS |
denotes |
clones |
| T12098 |
34-38 |
VBD |
denotes |
were |
| T12097 |
39-47 |
VBN |
denotes |
injected |
| T12099 |
48-52 |
IN |
denotes |
into |
| T12100 |
53-58 |
NN |
denotes |
C57BL |
| T12102 |
58-59 |
HYPH |
denotes |
/ |
| T12103 |
59-60 |
CD |
denotes |
6 |
| T12101 |
61-72 |
NNS |
denotes |
blastocysts |
| T12104 |
73-76 |
CC |
denotes |
and |
| T12105 |
77-88 |
VBN |
denotes |
transferred |
| T12106 |
89-93 |
IN |
denotes |
into |
| T12107 |
94-97 |
CD |
denotes |
2.5 |
| T12108 |
98-101 |
NN |
denotes |
dpc |
| T12110 |
102-116 |
JJ |
denotes |
pseudopregnant |
| T12109 |
117-127 |
NNS |
denotes |
recipients |
| T12111 |
128-130 |
IN |
denotes |
by |
| T12112 |
131-139 |
JJ |
denotes |
standard |
| T12113 |
140-150 |
NNS |
denotes |
techniques |
| T12114 |
150-151 |
. |
denotes |
. |
| T12115 |
151-230 |
sentence |
denotes |
Resulting chimeras were mated with C57BL/6 to establish germline transmission. |
| T12116 |
152-161 |
VBG |
denotes |
Resulting |
| T12117 |
162-170 |
NNS |
denotes |
chimeras |
| T12119 |
171-175 |
VBD |
denotes |
were |
| T12118 |
176-181 |
VBN |
denotes |
mated |
| T12120 |
182-186 |
IN |
denotes |
with |
| T12121 |
187-192 |
NN |
denotes |
C57BL |
| T12122 |
192-193 |
HYPH |
denotes |
/ |
| T12123 |
193-194 |
CD |
denotes |
6 |
| T12124 |
195-197 |
TO |
denotes |
to |
| T12125 |
198-207 |
VB |
denotes |
establish |
| T12126 |
208-216 |
NN |
denotes |
germline |
| T12127 |
217-229 |
NN |
denotes |
transmission |
| T12128 |
229-230 |
. |
denotes |
. |
| T12129 |
230-385 |
sentence |
denotes |
Offspring with the Atrx flox allele were identified by Southern blotting of SacI-digested tail DNA using the intron 17 probe as shown in Figure 2A and 2C. |
| T12130 |
231-240 |
NN |
denotes |
Offspring |
| T12132 |
241-245 |
IN |
denotes |
with |
| T12133 |
246-249 |
DT |
denotes |
the |
| T12135 |
250-259 |
NN |
denotes |
Atrx flox |
| T12134 |
260-266 |
NN |
denotes |
allele |
| T12136 |
267-271 |
VBD |
denotes |
were |
| T12131 |
272-282 |
VBN |
denotes |
identified |
| T12137 |
283-285 |
IN |
denotes |
by |
| T12138 |
286-294 |
NNP |
denotes |
Southern |
| T12139 |
295-303 |
NN |
denotes |
blotting |
| T12140 |
304-306 |
IN |
denotes |
of |
| T12141 |
307-311 |
NN |
denotes |
SacI |
| T12143 |
311-312 |
HYPH |
denotes |
- |
| T12142 |
312-320 |
VBN |
denotes |
digested |
| T12145 |
321-325 |
NN |
denotes |
tail |
| T12144 |
326-329 |
NN |
denotes |
DNA |
| T12146 |
330-335 |
VBG |
denotes |
using |
| T12147 |
336-339 |
DT |
denotes |
the |
| T12149 |
340-346 |
NN |
denotes |
intron |
| T12150 |
347-349 |
CD |
denotes |
17 |
| T12148 |
350-355 |
NN |
denotes |
probe |
| T12151 |
356-358 |
IN |
denotes |
as |
| T12152 |
359-364 |
VBN |
denotes |
shown |
| T12153 |
365-367 |
IN |
denotes |
in |
| T12154 |
368-374 |
NN |
denotes |
Figure |
| T12155 |
375-377 |
NN |
denotes |
2A |
| T12156 |
378-381 |
CC |
denotes |
and |
| T12157 |
382-384 |
NN |
denotes |
2C |
| T12158 |
384-385 |
. |
denotes |
. |
| T12159 |
385-493 |
sentence |
denotes |
For Cre-recombination, Atrx flox mice were crossed with GATA1-Cre transgenic mice as described in the text. |
| T12160 |
386-389 |
IN |
denotes |
For |
| T12162 |
390-393 |
NN |
denotes |
Cre |
| T12164 |
393-394 |
HYPH |
denotes |
- |
| T12163 |
394-407 |
NN |
denotes |
recombination |
| T12165 |
407-409 |
, |
denotes |
, |
| T12166 |
409-418 |
NN |
denotes |
Atrx flox |
| T12167 |
419-423 |
NNS |
denotes |
mice |
| T12168 |
424-428 |
VBD |
denotes |
were |
| T12161 |
429-436 |
VBN |
denotes |
crossed |
| T12169 |
437-441 |
IN |
denotes |
with |
| T12170 |
442-447 |
NN |
denotes |
GATA1 |
| T12172 |
447-448 |
HYPH |
denotes |
- |
| T12171 |
448-451 |
NN |
denotes |
Cre |
| T12173 |
452-462 |
JJ |
denotes |
transgenic |
| T12174 |
463-467 |
NNS |
denotes |
mice |
| T12175 |
468-470 |
IN |
denotes |
as |
| T12176 |
471-480 |
VBN |
denotes |
described |
| T12177 |
481-483 |
IN |
denotes |
in |
| T12178 |
484-487 |
DT |
denotes |
the |
| T12179 |
488-492 |
NN |
denotes |
text |
| T12180 |
492-493 |
. |
denotes |
. |
| T12181 |
493-606 |
sentence |
denotes |
Recombinant alleles were detected by Southern blotting as described above or by PCR as described in Protocol S1. |
| T12182 |
494-505 |
JJ |
denotes |
Recombinant |
| T12183 |
506-513 |
NNS |
denotes |
alleles |
| T12185 |
514-518 |
VBD |
denotes |
were |
| T12184 |
519-527 |
VBN |
denotes |
detected |
| T12186 |
528-530 |
IN |
denotes |
by |
| T12187 |
531-539 |
NNP |
denotes |
Southern |
| T12188 |
540-548 |
NN |
denotes |
blotting |
| T12189 |
549-551 |
IN |
denotes |
as |
| T12190 |
552-561 |
VBN |
denotes |
described |
| T12191 |
562-567 |
RB |
denotes |
above |
| T12192 |
568-570 |
CC |
denotes |
or |
| T12193 |
571-573 |
IN |
denotes |
by |
| T12194 |
574-577 |
NN |
denotes |
PCR |
| T12195 |
578-580 |
IN |
denotes |
as |
| T12196 |
581-590 |
VBN |
denotes |
described |
| T12197 |
591-593 |
IN |
denotes |
in |
| T12198 |
594-602 |
NN |
denotes |
Protocol |
| T12199 |
603-605 |
NN |
denotes |
S1 |
| T12200 |
605-606 |
. |
denotes |
. |
| R7562 |
T12092 |
T12093 |
amod |
Targeted,clones |
| R7563 |
T12093 |
T12097 |
nsubjpass |
clones,injected |
| R7564 |
T12094 |
T12095 |
compound |
Atrx flox,cell |
| R7565 |
T12095 |
T12093 |
compound |
cell,clones |
| R7566 |
T12096 |
T12095 |
compound |
ES,cell |
| R7567 |
T12098 |
T12097 |
auxpass |
were,injected |
| R7568 |
T12099 |
T12097 |
prep |
into,injected |
| R7569 |
T12100 |
T12101 |
nmod |
C57BL,blastocysts |
| R7570 |
T12101 |
T12099 |
pobj |
blastocysts,into |
| R7571 |
T12102 |
T12100 |
punct |
/,C57BL |
| R7572 |
T12103 |
T12100 |
nummod |
6,C57BL |
| R7573 |
T12104 |
T12097 |
cc |
and,injected |
| R7574 |
T12105 |
T12097 |
conj |
transferred,injected |
| R7575 |
T12106 |
T12105 |
prep |
into,transferred |
| R7576 |
T12107 |
T12108 |
nummod |
2.5,dpc |
| R7577 |
T12108 |
T12109 |
nmod |
dpc,recipients |
| R7578 |
T12109 |
T12106 |
pobj |
recipients,into |
| R7579 |
T12110 |
T12109 |
amod |
pseudopregnant,recipients |
| R7580 |
T12111 |
T12097 |
prep |
by,injected |
| R7581 |
T12112 |
T12113 |
amod |
standard,techniques |
| R7582 |
T12113 |
T12111 |
pobj |
techniques,by |
| R7583 |
T12114 |
T12097 |
punct |
.,injected |
| R7584 |
T12116 |
T12117 |
amod |
Resulting,chimeras |
| R7585 |
T12117 |
T12118 |
nsubjpass |
chimeras,mated |
| R7586 |
T12119 |
T12118 |
auxpass |
were,mated |
| R7587 |
T12120 |
T12118 |
prep |
with,mated |
| R7588 |
T12121 |
T12120 |
pobj |
C57BL,with |
| R7589 |
T12122 |
T12121 |
punct |
/,C57BL |
| R7590 |
T12123 |
T12121 |
nummod |
6,C57BL |
| R7591 |
T12124 |
T12125 |
aux |
to,establish |
| R7592 |
T12125 |
T12118 |
advcl |
establish,mated |
| R7593 |
T12126 |
T12127 |
compound |
germline,transmission |
| R7594 |
T12127 |
T12125 |
dobj |
transmission,establish |
| R7595 |
T12128 |
T12118 |
punct |
.,mated |
| R7596 |
T12130 |
T12131 |
nsubjpass |
Offspring,identified |
| R7597 |
T12132 |
T12130 |
prep |
with,Offspring |
| R7598 |
T12133 |
T12134 |
det |
the,allele |
| R7599 |
T12134 |
T12132 |
pobj |
allele,with |
| R7600 |
T12135 |
T12134 |
compound |
Atrx flox,allele |
| R7601 |
T12136 |
T12131 |
auxpass |
were,identified |
| R7602 |
T12137 |
T12131 |
prep |
by,identified |
| R7603 |
T12138 |
T12139 |
compound |
Southern,blotting |
| R7604 |
T12139 |
T12137 |
pobj |
blotting,by |
| R7605 |
T12140 |
T12139 |
prep |
of,blotting |
| R7606 |
T12141 |
T12142 |
npadvmod |
SacI,digested |
| R7607 |
T12142 |
T12144 |
amod |
digested,DNA |
| R7608 |
T12143 |
T12142 |
punct |
-,digested |
| R7609 |
T12144 |
T12140 |
pobj |
DNA,of |
| R7610 |
T12145 |
T12144 |
compound |
tail,DNA |
| R7611 |
T12146 |
T12131 |
advcl |
using,identified |
| R7612 |
T12147 |
T12148 |
det |
the,probe |
| R7613 |
T12148 |
T12146 |
dobj |
probe,using |
| R7614 |
T12149 |
T12148 |
nmod |
intron,probe |
| R7615 |
T12150 |
T12149 |
nummod |
17,intron |
| R7616 |
T12151 |
T12152 |
mark |
as,shown |
| R7617 |
T12152 |
T12146 |
advcl |
shown,using |
| R7618 |
T12153 |
T12152 |
prep |
in,shown |
| R7619 |
T12154 |
T12155 |
compound |
Figure,2A |
| R7620 |
T12155 |
T12153 |
pobj |
2A,in |
| R7621 |
T12156 |
T12155 |
cc |
and,2A |
| R7622 |
T12157 |
T12155 |
conj |
2C,2A |
| R7623 |
T12158 |
T12131 |
punct |
.,identified |
| R7624 |
T12160 |
T12161 |
prep |
For,crossed |
| R7625 |
T12162 |
T12163 |
compound |
Cre,recombination |
| R7626 |
T12163 |
T12160 |
pobj |
recombination,For |
| R7627 |
T12164 |
T12163 |
punct |
-,recombination |
| R7628 |
T12165 |
T12161 |
punct |
", ",crossed |
| R7629 |
T12166 |
T12167 |
compound |
Atrx flox,mice |
| R7630 |
T12167 |
T12161 |
nsubjpass |
mice,crossed |
| R7631 |
T12168 |
T12161 |
auxpass |
were,crossed |
| R7632 |
T12169 |
T12161 |
prep |
with,crossed |
| R7633 |
T12170 |
T12171 |
compound |
GATA1,Cre |
| R7634 |
T12171 |
T12173 |
npadvmod |
Cre,transgenic |
| R7635 |
T12172 |
T12171 |
punct |
-,Cre |
| R7636 |
T12173 |
T12174 |
amod |
transgenic,mice |
| R7637 |
T12174 |
T12169 |
pobj |
mice,with |
| R7638 |
T12175 |
T12176 |
mark |
as,described |
| R7639 |
T12176 |
T12161 |
advcl |
described,crossed |
| R7640 |
T12177 |
T12176 |
prep |
in,described |
| R7641 |
T12178 |
T12179 |
det |
the,text |
| R7642 |
T12179 |
T12177 |
pobj |
text,in |
| R7643 |
T12180 |
T12161 |
punct |
.,crossed |
| R7644 |
T12182 |
T12183 |
amod |
Recombinant,alleles |
| R7645 |
T12183 |
T12184 |
nsubjpass |
alleles,detected |
| R7646 |
T12185 |
T12184 |
auxpass |
were,detected |
| R7647 |
T12186 |
T12184 |
prep |
by,detected |
| R7648 |
T12187 |
T12188 |
compound |
Southern,blotting |
| R7649 |
T12188 |
T12186 |
pobj |
blotting,by |
| R7650 |
T12189 |
T12190 |
mark |
as,described |
| R7651 |
T12190 |
T12188 |
advcl |
described,blotting |
| R7652 |
T12191 |
T12190 |
advmod |
above,described |
| R7653 |
T12192 |
T12186 |
cc |
or,by |
| R7654 |
T12193 |
T12186 |
conj |
by,by |
| R7655 |
T12194 |
T12193 |
pobj |
PCR,by |
| R7656 |
T12195 |
T12196 |
mark |
as,described |
| R7657 |
T12196 |
T12184 |
advcl |
described,detected |
| R7658 |
T12197 |
T12196 |
prep |
in,described |
| R7659 |
T12198 |
T12199 |
compound |
Protocol,S1 |
| R7660 |
T12199 |
T12197 |
pobj |
S1,in |
| R7661 |
T12200 |
T12184 |
punct |
.,detected |
