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PMC:1440874 / 2855-60642 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T722 13-16 NN denotes ATR
T724 16-17 HYPH denotes -
T723 17-18 NN denotes X
T725 19-27 NN denotes syndrome
T726 28-30 VBZ denotes is
T727 31-32 DT denotes a
T729 33-39 JJ denotes severe
T730 39-41 , denotes ,
T731 41-55 JJ denotes nonprogressive
T728 56-60 NN denotes form
T732 61-63 IN denotes of
T733 64-65 NN denotes X
T735 65-66 HYPH denotes -
T734 66-72 VBN denotes linked
T737 73-79 JJ denotes mental
T736 80-91 NN denotes retardation
T738 92-96 WDT denotes that
T740 97-99 VBZ denotes is
T741 100-110 RB denotes frequently
T739 111-121 VBN denotes associated
T742 122-126 IN denotes with
T743 127-135 JJ denotes multiple
T745 136-146 JJ denotes congenital
T744 147-160 NNS denotes abnormalities
T746 161-162 -LRB- denotes [
T747 162-163 CD denotes 1
T748 163-164 -RRB- denotes ]
T749 164-165 . denotes .
T750 165-414 sentence denotes It is usually associated with a mild form of α-thalassaemia, caused by reduced expression of structurally intact α-globin genes, and characterised by the presence of β-globin tetramers (haemoglobin H inclusion bodies) in peripheral red blood cells.
T751 166-168 PRP denotes It
T753 169-171 VBZ denotes is
T754 172-179 RB denotes usually
T752 180-190 VBN denotes associated
T755 191-195 IN denotes with
T756 196-197 DT denotes a
T758 198-202 JJ denotes mild
T757 203-207 NN denotes form
T759 208-210 IN denotes of
T760 211-212 NN denotes α
T762 212-213 HYPH denotes -
T761 213-225 NN denotes thalassaemia
T763 225-227 , denotes ,
T764 227-233 VBN denotes caused
T765 234-236 IN denotes by
T766 237-244 VBN denotes reduced
T767 245-255 NN denotes expression
T768 256-258 IN denotes of
T769 259-271 RB denotes structurally
T770 272-278 JJ denotes intact
T772 279-280 NN denotes α
T774 280-281 HYPH denotes -
T773 281-287 NN denotes globin
T771 288-293 NNS denotes genes
T775 293-295 , denotes ,
T776 295-298 CC denotes and
T777 299-312 VBN denotes characterised
T778 313-315 IN denotes by
T779 316-319 DT denotes the
T780 320-328 NN denotes presence
T781 329-331 IN denotes of
T782 332-333 NN denotes β
T784 333-334 HYPH denotes -
T783 334-340 NN denotes globin
T785 341-350 NNS denotes tetramers
T786 351-352 -LRB- denotes (
T787 352-363 NN denotes haemoglobin
T788 364-365 NN denotes H
T790 366-375 NN denotes inclusion
T789 376-382 NNS denotes bodies
T791 382-383 -RRB- denotes )
T792 384-386 IN denotes in
T793 387-397 JJ denotes peripheral
T795 398-401 JJ denotes red
T796 402-407 NN denotes blood
T794 408-413 NNS denotes cells
T797 413-414 . denotes .
T798 414-534 sentence denotes Carrier females occasionally manifest haemoglobin H inclusions, but are otherwise intellectually and physically normal.
T799 415-422 NN denotes Carrier
T800 423-430 NNS denotes females
T802 431-443 RB denotes occasionally
T801 444-452 VBP denotes manifest
T803 453-464 NN denotes haemoglobin
T804 465-466 NN denotes H
T805 467-477 NNS denotes inclusions
T806 477-479 , denotes ,
T807 479-482 CC denotes but
T808 483-486 VBP denotes are
T809 487-496 RB denotes otherwise
T810 497-511 RB denotes intellectually
T812 512-515 CC denotes and
T813 516-526 RB denotes physically
T811 527-533 JJ denotes normal
T814 533-534 . denotes .
T815 534-807 sentence denotes Studies of X-chromosome inactivation in carrier females have demonstrated preferential inactivation of the chromosome bearing the abnormal allele in a variety of tissues [2], and this skewing of X-inactivation is thought to explain the mild phenotype observed in carriers.
T816 535-542 NNS denotes Studies
T818 543-545 IN denotes of
T819 546-547 NN denotes X
T821 547-548 HYPH denotes -
T820 548-558 NN denotes chromosome
T822 559-571 NN denotes inactivation
T823 572-574 IN denotes in
T824 575-582 NN denotes carrier
T825 583-590 NNS denotes females
T826 591-595 VBP denotes have
T817 596-608 VBN denotes demonstrated
T827 609-621 JJ denotes preferential
T828 622-634 NN denotes inactivation
T829 635-637 IN denotes of
T830 638-641 DT denotes the
T831 642-652 NN denotes chromosome
T832 653-660 VBG denotes bearing
T833 661-664 DT denotes the
T835 665-673 JJ denotes abnormal
T834 674-680 NN denotes allele
T836 681-683 IN denotes in
T837 684-685 DT denotes a
T838 686-693 NN denotes variety
T839 694-696 IN denotes of
T840 697-704 NNS denotes tissues
T841 705-706 -LRB- denotes [
T842 706-707 CD denotes 2
T843 707-708 -RRB- denotes ]
T844 708-710 , denotes ,
T845 710-713 CC denotes and
T846 714-718 DT denotes this
T847 719-726 NN denotes skewing
T849 727-729 IN denotes of
T850 730-731 NN denotes X
T852 731-732 HYPH denotes -
T851 732-744 NN denotes inactivation
T853 745-747 VBZ denotes is
T848 748-755 VBN denotes thought
T854 756-758 TO denotes to
T855 759-766 VB denotes explain
T856 767-770 DT denotes the
T858 771-775 JJ denotes mild
T857 776-785 NN denotes phenotype
T859 786-794 VBN denotes observed
T860 795-797 IN denotes in
T861 798-806 NNS denotes carriers
T862 806-807 . denotes .
T863 807-947 sentence denotes The ATR-X syndrome is caused by mutations in a gene (ATRX) that comprises 36 exons spanning 300 kb of genomic DNA at Chromosome Xq13.3 [3].
T864 808-811 DT denotes The
T866 812-815 NN denotes ATR
T868 815-816 HYPH denotes -
T867 816-817 NN denotes X
T865 818-826 NN denotes syndrome
T870 827-829 VBZ denotes is
T869 830-836 VBN denotes caused
T871 837-839 IN denotes by
T872 840-849 NNS denotes mutations
T873 850-852 IN denotes in
T874 853-854 DT denotes a
T875 855-859 NN denotes gene
T876 860-861 -LRB- denotes (
T877 861-865 NN denotes ATRX
T878 865-866 -RRB- denotes )
T879 867-871 WDT denotes that
T880 872-881 VBZ denotes comprises
T881 882-884 CD denotes 36
T882 885-890 NNS denotes exons
T883 891-899 VBG denotes spanning
T884 900-903 CD denotes 300
T885 904-906 NNS denotes kb
T886 907-909 IN denotes of
T887 910-917 JJ denotes genomic
T888 918-921 NN denotes DNA
T889 922-924 IN denotes at
T890 925-935 NN denotes Chromosome
T891 936-942 NN denotes Xq13.3
T892 943-944 -LRB- denotes [
T893 944-945 CD denotes 3
T894 945-946 -RRB- denotes ]
T895 946-947 . denotes .
T896 947-1007 sentence denotes This gene encodes two dominant protein isoforms (Figure 1).
T897 948-952 DT denotes This
T898 953-957 NN denotes gene
T899 958-965 VBZ denotes encodes
T900 966-969 CD denotes two
T902 970-978 JJ denotes dominant
T903 979-986 NN denotes protein
T901 987-995 NNS denotes isoforms
T904 996-997 -LRB- denotes (
T905 997-1003 NN denotes Figure
T906 1004-1005 CD denotes 1
T907 1005-1006 -RRB- denotes )
T908 1006-1007 . denotes .
T909 1007-1356 sentence denotes As well as the full-length ATRX protein of ~280 kDa, which is encoded by a transcript of ~10 kb, we recently demonstrated that a truncated isoform called ATRXt (~200 kDa) is produced from a transcript of around 7 kb, which arises when intron 11 fails to be spliced from the primary transcript and an alternative intronic poly(A) signal is used [4].
T910 1008-1010 RB denotes As
T912 1011-1015 RB denotes well
T911 1016-1018 IN denotes as
T914 1019-1022 DT denotes the
T916 1023-1027 JJ denotes full
T918 1027-1028 HYPH denotes -
T917 1028-1034 NN denotes length
T919 1035-1039 NN denotes ATRX
T915 1040-1047 NN denotes protein
T920 1048-1050 IN denotes of
T921 1051-1052 SYM denotes ~
T922 1052-1055 CD denotes 280
T923 1056-1059 NN denotes kDa
T924 1059-1061 , denotes ,
T925 1061-1066 WDT denotes which
T927 1067-1069 VBZ denotes is
T926 1070-1077 VBN denotes encoded
T928 1078-1080 IN denotes by
T929 1081-1082 DT denotes a
T930 1083-1093 NN denotes transcript
T931 1094-1096 IN denotes of
T932 1097-1098 SYM denotes ~
T933 1098-1100 CD denotes 10
T934 1101-1103 NN denotes kb
T935 1103-1105 , denotes ,
T936 1105-1107 PRP denotes we
T937 1108-1116 RB denotes recently
T913 1117-1129 VBD denotes demonstrated
T938 1130-1134 IN denotes that
T940 1135-1136 DT denotes a
T942 1137-1146 VBN denotes truncated
T941 1147-1154 NN denotes isoform
T943 1155-1161 VBN denotes called
T944 1162-1167 NN denotes ATRXt
T945 1168-1169 -LRB- denotes (
T947 1169-1170 SYM denotes ~
T948 1170-1173 CD denotes 200
T946 1174-1177 NN denotes kDa
T949 1177-1178 -RRB- denotes )
T950 1179-1181 VBZ denotes is
T939 1182-1190 VBN denotes produced
T951 1191-1195 IN denotes from
T952 1196-1197 DT denotes a
T953 1198-1208 NN denotes transcript
T954 1209-1211 IN denotes of
T955 1212-1218 IN denotes around
T956 1219-1220 CD denotes 7
T957 1221-1223 NN denotes kb
T958 1223-1225 , denotes ,
T959 1225-1230 WDT denotes which
T960 1231-1237 VBZ denotes arises
T961 1238-1242 WRB denotes when
T963 1243-1249 NN denotes intron
T964 1250-1252 CD denotes 11
T962 1253-1258 VBZ denotes fails
T965 1259-1261 TO denotes to
T967 1262-1264 VB denotes be
T966 1265-1272 VBN denotes spliced
T968 1273-1277 IN denotes from
T969 1278-1281 DT denotes the
T971 1282-1289 JJ denotes primary
T970 1290-1300 NN denotes transcript
T972 1301-1304 CC denotes and
T973 1305-1307 DT denotes an
T975 1308-1319 JJ denotes alternative
T976 1320-1328 JJ denotes intronic
T977 1329-1333 NN denotes poly
T978 1333-1334 -LRB- denotes (
T979 1334-1335 NN denotes A
T980 1335-1336 -RRB- denotes )
T974 1337-1343 NN denotes signal
T982 1344-1346 VBZ denotes is
T981 1347-1351 VBN denotes used
T983 1352-1353 -LRB- denotes [
T984 1353-1354 CD denotes 4
T985 1354-1355 -RRB- denotes ]
T986 1355-1356 . denotes .
T987 1356-1536 sentence denotes The mouse homolog of the ATRX gene, Atrx, is also situated on the X chromosome, and also gives rise to full-length (Atrx, ~280 kDa) and truncated (Atrxt, ~200 kDa) isoforms [4,5].
T988 1357-1360 DT denotes The
T990 1361-1366 NN denotes mouse
T989 1367-1374 NN denotes homolog
T992 1375-1377 IN denotes of
T993 1378-1381 DT denotes the
T995 1382-1386 NN denotes ATRX
T994 1387-1391 NN denotes gene
T996 1391-1393 , denotes ,
T997 1393-1397 NN denotes Atrx
T998 1397-1399 , denotes ,
T999 1399-1401 VBZ denotes is
T1000 1402-1406 RB denotes also
T991 1407-1415 VBN denotes situated
T1001 1416-1418 IN denotes on
T1002 1419-1422 DT denotes the
T1004 1423-1424 NN denotes X
T1003 1425-1435 NN denotes chromosome
T1005 1435-1437 , denotes ,
T1006 1437-1440 CC denotes and
T1007 1441-1445 RB denotes also
T1008 1446-1451 VBZ denotes gives
T1009 1452-1456 NN denotes rise
T1010 1457-1459 IN denotes to
T1011 1460-1464 JJ denotes full
T1013 1464-1465 HYPH denotes -
T1012 1465-1471 NN denotes length
T1015 1472-1473 -LRB- denotes (
T1017 1473-1477 NN denotes Atrx
T1018 1477-1479 , denotes ,
T1019 1479-1480 SYM denotes ~
T1020 1480-1483 CD denotes 280
T1016 1484-1487 NN denotes kDa
T1021 1487-1488 -RRB- denotes )
T1022 1489-1492 CC denotes and
T1023 1493-1502 VBN denotes truncated
T1024 1503-1504 -LRB- denotes (
T1026 1504-1509 NN denotes Atrxt
T1027 1509-1511 , denotes ,
T1028 1511-1512 SYM denotes ~
T1029 1512-1515 CD denotes 200
T1025 1516-1519 NN denotes kDa
T1030 1519-1520 -RRB- denotes )
T1014 1521-1529 NNS denotes isoforms
T1031 1530-1531 -LRB- denotes [
T1033 1531-1532 CD denotes 4
T1034 1532-1533 , denotes ,
T1032 1533-1534 CD denotes 5
T1035 1534-1535 -RRB- denotes ]
T1036 1535-1536 . denotes .
T1037 1536-2546 sentence denotes Figure 1 Schematic Representation of the ATRX Isoforms Shown at the top is the human ATRX cDNA. The boxes represent the 36 exons. The introns are not to scale. The alternative splicing of exons 6 and 7 is indicated. Shown underneath are the two ATRX protein isoforms. Full-length ATRX (~280 kDa) is encoded by the largest open reading frame. The positions of the principal features (the PHD-like domain and the seven SWI/SNF-like motifs) are indicated. Above full-length ATRX is shown the truncated ATRXt isoform (apparent molecular weight of ~200 kDa) that arises through the failure to splice intron 11 and the use of an intronic poly(A) signal. The intron-encoded region of ATRXt is indicated as a filled grey box. Locations of recombinant proteins (A2, FXNP5, and H-300) used to generate antibodies are shown. The scale bar represents 200 amino acids. Disease-causing missense mutations are clustered in two regions of the gene: a PHD-like zinc finger domain and a SNF2-like ATPase domain (Figure 1) [6].
T1038 2394-2401 NN denotes Disease
T1040 2401-2402 HYPH denotes -
T1039 2402-2409 VBG denotes causing
T1042 2410-2418 NN denotes missense
T1041 2419-2428 NNS denotes mutations
T1044 2429-2432 VBP denotes are
T1043 2433-2442 VBN denotes clustered
T1045 2443-2445 IN denotes in
T1046 2446-2449 CD denotes two
T1047 2450-2457 NNS denotes regions
T1048 2458-2460 IN denotes of
T1049 2461-2464 DT denotes the
T1050 2465-2469 NN denotes gene
T1051 2469-2471 : denotes :
T1052 2471-2472 DT denotes a
T1054 2473-2476 NN denotes PHD
T1056 2476-2477 HYPH denotes -
T1055 2477-2481 JJ denotes like
T1057 2482-2486 NN denotes zinc
T1058 2487-2493 NN denotes finger
T1053 2494-2500 NN denotes domain
T1059 2501-2504 CC denotes and
T1060 2505-2506 DT denotes a
T1062 2507-2511 NN denotes SNF2
T1064 2511-2512 HYPH denotes -
T1063 2512-2516 JJ denotes like
T1065 2517-2523 NN denotes ATPase
T1061 2524-2530 NN denotes domain
T1066 2531-2532 -LRB- denotes (
T1067 2532-2538 NN denotes Figure
T1068 2539-2540 CD denotes 1
T1069 2540-2541 -RRB- denotes )
T1070 2542-2543 -LRB- denotes [
T1071 2543-2544 CD denotes 6
T1072 2544-2545 -RRB- denotes ]
T1073 2545-2546 . denotes .
T1074 2546-2802 sentence denotes The former motif is thought to be involved in protein-protein interactions in chromatin [7], and the latter is a feature of chromatin-remodelling proteins, and the presence of disease-causing mutations indicates the functional importance of these domains.
T1075 2547-2550 DT denotes The
T1077 2551-2557 JJ denotes former
T1076 2558-2563 NN denotes motif
T1079 2564-2566 VBZ denotes is
T1078 2567-2574 VBN denotes thought
T1080 2575-2577 TO denotes to
T1082 2578-2580 VB denotes be
T1081 2581-2589 VBN denotes involved
T1083 2590-2592 IN denotes in
T1084 2593-2600 NN denotes protein
T1086 2600-2601 SYM denotes -
T1085 2601-2608 NN denotes protein
T1087 2609-2621 NNS denotes interactions
T1088 2622-2624 IN denotes in
T1089 2625-2634 NN denotes chromatin
T1090 2635-2636 -LRB- denotes [
T1091 2636-2637 CD denotes 7
T1092 2637-2638 -RRB- denotes ]
T1093 2638-2640 , denotes ,
T1094 2640-2643 CC denotes and
T1095 2644-2647 DT denotes the
T1096 2648-2654 JJ denotes latter
T1097 2655-2657 VBZ denotes is
T1098 2658-2659 DT denotes a
T1099 2660-2667 NN denotes feature
T1100 2668-2670 IN denotes of
T1101 2671-2680 NN denotes chromatin
T1103 2680-2681 HYPH denotes -
T1102 2681-2692 VBG denotes remodelling
T1104 2693-2701 NN denotes proteins
T1105 2701-2703 , denotes ,
T1106 2703-2706 CC denotes and
T1107 2707-2710 DT denotes the
T1108 2711-2719 NN denotes presence
T1110 2720-2722 IN denotes of
T1111 2723-2730 NN denotes disease
T1113 2730-2731 HYPH denotes -
T1112 2731-2738 VBG denotes causing
T1114 2739-2748 NNS denotes mutations
T1109 2749-2758 VBZ denotes indicates
T1115 2759-2762 DT denotes the
T1117 2763-2773 JJ denotes functional
T1116 2774-2784 NN denotes importance
T1118 2785-2787 IN denotes of
T1119 2788-2793 DT denotes these
T1120 2794-2801 NNS denotes domains
T1121 2801-2802 . denotes .
T1122 2802-2848 sentence denotes ATRX has been shown to remodel chromatin [8].
T1123 2803-2807 NN denotes ATRX
T1125 2808-2811 VBZ denotes has
T1126 2812-2816 VBN denotes been
T1124 2817-2822 VBN denotes shown
T1127 2823-2825 TO denotes to
T1128 2826-2833 VB denotes remodel
T1129 2834-2843 NN denotes chromatin
T1130 2844-2845 -LRB- denotes [
T1131 2845-2846 CD denotes 8
T1132 2846-2847 -RRB- denotes ]
T1133 2847-2848 . denotes .
T1134 2848-2991 sentence denotes It also interacts with HP1 at heterochromatin [9] and is recruited to promyelocytic leukemia nuclear bodies via an interaction with Daxx [10].
T1135 2849-2851 PRP denotes It
T1137 2852-2856 RB denotes also
T1136 2857-2866 VBZ denotes interacts
T1138 2867-2871 IN denotes with
T1139 2872-2875 NN denotes HP1
T1140 2876-2878 IN denotes at
T1141 2879-2894 NN denotes heterochromatin
T1142 2895-2896 -LRB- denotes [
T1143 2896-2897 CD denotes 9
T1144 2897-2898 -RRB- denotes ]
T1145 2899-2902 CC denotes and
T1146 2903-2905 VBZ denotes is
T1147 2906-2915 VBN denotes recruited
T1148 2916-2918 IN denotes to
T1149 2919-2932 JJ denotes promyelocytic
T1150 2933-2941 NN denotes leukemia
T1152 2942-2949 JJ denotes nuclear
T1151 2950-2956 NNS denotes bodies
T1153 2957-2960 IN denotes via
T1154 2961-2963 DT denotes an
T1155 2964-2975 NN denotes interaction
T1156 2976-2980 IN denotes with
T1157 2981-2985 NN denotes Daxx
T1158 2986-2987 -LRB- denotes [
T1159 2987-2989 CD denotes 10
T1160 2989-2990 -RRB- denotes ]
T1161 2990-2991 . denotes .
T1162 2991-3073 sentence denotes Furthermore, disruption of ATRX leads to diverse changes in DNA methylation [11].
T1163 2992-3003 RB denotes Furthermore
T1165 3003-3005 , denotes ,
T1166 3005-3015 NN denotes disruption
T1167 3016-3018 IN denotes of
T1168 3019-3023 NN denotes ATRX
T1164 3024-3029 VBZ denotes leads
T1169 3030-3032 IN denotes to
T1170 3033-3040 JJ denotes diverse
T1171 3041-3048 NNS denotes changes
T1172 3049-3051 IN denotes in
T1173 3052-3055 NN denotes DNA
T1174 3056-3067 NN denotes methylation
T1175 3068-3069 -LRB- denotes [
T1176 3069-3071 CD denotes 11
T1177 3071-3072 -RRB- denotes ]
T1178 3072-3073 . denotes .
T1179 3073-3143 sentence denotes Nevertheless, the role ATRX plays in gene expression remains unclear.
T1180 3074-3086 RB denotes Nevertheless
T1182 3086-3088 , denotes ,
T1183 3088-3091 DT denotes the
T1184 3092-3096 NN denotes role
T1185 3097-3101 NN denotes ATRX
T1186 3102-3107 VBZ denotes plays
T1187 3108-3110 IN denotes in
T1188 3111-3115 NN denotes gene
T1189 3116-3126 NN denotes expression
T1181 3127-3134 VBZ denotes remains
T1190 3135-3142 JJ denotes unclear
T1191 3142-3143 . denotes .
T1192 3143-3368 sentence denotes The consistent core of clinical and haematological features observed in ATR-X patients suggests that, like the SWI2/SNF2 chromatin-remodelling protein, ATRX probably regulates transcription of a discrete set of target genes.
T1193 3144-3147 DT denotes The
T1195 3148-3158 JJ denotes consistent
T1194 3159-3163 NN denotes core
T1197 3164-3166 IN denotes of
T1198 3167-3175 JJ denotes clinical
T1200 3176-3179 CC denotes and
T1201 3180-3194 JJ denotes haematological
T1199 3195-3203 NNS denotes features
T1202 3204-3212 VBN denotes observed
T1203 3213-3215 IN denotes in
T1204 3216-3219 NN denotes ATR
T1206 3219-3220 HYPH denotes -
T1205 3220-3221 NN denotes X
T1207 3222-3230 NNS denotes patients
T1196 3231-3239 VBZ denotes suggests
T1208 3240-3244 IN denotes that
T1210 3244-3246 , denotes ,
T1211 3246-3250 IN denotes like
T1212 3251-3254 DT denotes the
T1214 3255-3259 NN denotes SWI2
T1216 3259-3260 HYPH denotes /
T1215 3260-3264 NN denotes SNF2
T1217 3265-3274 NN denotes chromatin
T1219 3274-3275 HYPH denotes -
T1218 3275-3286 VBG denotes remodelling
T1213 3287-3294 NN denotes protein
T1220 3294-3296 , denotes ,
T1221 3296-3300 NN denotes ATRX
T1222 3301-3309 RB denotes probably
T1209 3310-3319 VBZ denotes regulates
T1223 3320-3333 NN denotes transcription
T1224 3334-3336 IN denotes of
T1225 3337-3338 DT denotes a
T1227 3339-3347 JJ denotes discrete
T1226 3348-3351 NN denotes set
T1228 3352-3354 IN denotes of
T1229 3355-3361 NN denotes target
T1230 3362-3367 NNS denotes genes
T1231 3367-3368 . denotes .
T1232 3368-3528 sentence denotes However, although there are clearly others to be found, at present the α-globin genes remain the only confirmed targets for transcriptional regulation by ATRX.
T1233 3369-3376 RB denotes However
T1235 3376-3378 , denotes ,
T1236 3378-3386 IN denotes although
T1238 3387-3392 EX denotes there
T1237 3393-3396 VBP denotes are
T1239 3397-3404 RB denotes clearly
T1240 3405-3411 NNS denotes others
T1241 3412-3414 TO denotes to
T1243 3415-3417 VB denotes be
T1242 3418-3423 VBN denotes found
T1244 3423-3425 , denotes ,
T1245 3425-3427 IN denotes at
T1246 3428-3435 JJ denotes present
T1247 3436-3439 DT denotes the
T1249 3440-3441 NN denotes α
T1251 3441-3442 HYPH denotes -
T1250 3442-3448 NN denotes globin
T1248 3449-3454 NNS denotes genes
T1234 3455-3461 VBP denotes remain
T1252 3462-3465 DT denotes the
T1254 3466-3470 RB denotes only
T1255 3471-3480 VBN denotes confirmed
T1253 3481-3488 NNS denotes targets
T1256 3489-3492 IN denotes for
T1257 3493-3508 JJ denotes transcriptional
T1258 3509-3519 NN denotes regulation
T1259 3520-3522 IN denotes by
T1260 3523-3527 NN denotes ATRX
T1261 3527-3528 . denotes .
T1262 3528-3627 sentence denotes Little is currently known about the precise role of the ATRX protein during mammalian development.
T1263 3529-3535 JJ denotes Little
T1265 3536-3538 VBZ denotes is
T1266 3539-3548 RB denotes currently
T1264 3549-3554 VBN denotes known
T1267 3555-3560 IN denotes about
T1268 3561-3564 DT denotes the
T1270 3565-3572 JJ denotes precise
T1269 3573-3577 NN denotes role
T1271 3578-3580 IN denotes of
T1272 3581-3584 DT denotes the
T1274 3585-3589 NN denotes ATRX
T1273 3590-3597 NN denotes protein
T1275 3598-3604 IN denotes during
T1276 3605-3614 JJ denotes mammalian
T1277 3615-3626 NN denotes development
T1278 3626-3627 . denotes .
T1279 3627-3922 sentence denotes To investigate the role of this protein during mouse development, we generated a conditionally deleted allele of the Atrx gene in mouse embryonic stem (ES) cells, and used these cells to examine the effect of ablating expression of the full-length Atrx protein in ES cells and in mouse embryos.
T1280 3628-3630 TO denotes To
T1281 3631-3642 VB denotes investigate
T1283 3643-3646 DT denotes the
T1284 3647-3651 NN denotes role
T1285 3652-3654 IN denotes of
T1286 3655-3659 DT denotes this
T1287 3660-3667 NN denotes protein
T1288 3668-3674 IN denotes during
T1289 3675-3680 NN denotes mouse
T1290 3681-3692 NN denotes development
T1291 3692-3694 , denotes ,
T1292 3694-3696 PRP denotes we
T1282 3697-3706 VBD denotes generated
T1293 3707-3708 DT denotes a
T1295 3709-3722 RB denotes conditionally
T1296 3723-3730 VBN denotes deleted
T1294 3731-3737 NN denotes allele
T1297 3738-3740 IN denotes of
T1298 3741-3744 DT denotes the
T1300 3745-3749 NN denotes Atrx
T1299 3750-3754 NN denotes gene
T1301 3755-3757 IN denotes in
T1302 3758-3763 NN denotes mouse
T1304 3764-3773 JJ denotes embryonic
T1305 3774-3778 NN denotes stem
T1306 3779-3780 -LRB- denotes (
T1307 3780-3782 NN denotes ES
T1308 3782-3783 -RRB- denotes )
T1303 3784-3789 NNS denotes cells
T1309 3789-3791 , denotes ,
T1310 3791-3794 CC denotes and
T1311 3795-3799 VBD denotes used
T1312 3800-3805 DT denotes these
T1313 3806-3811 NNS denotes cells
T1314 3812-3814 TO denotes to
T1315 3815-3822 VB denotes examine
T1316 3823-3826 DT denotes the
T1317 3827-3833 NN denotes effect
T1318 3834-3836 IN denotes of
T1319 3837-3845 VBG denotes ablating
T1320 3846-3856 NN denotes expression
T1321 3857-3859 IN denotes of
T1322 3860-3863 DT denotes the
T1324 3864-3868 JJ denotes full
T1326 3868-3869 HYPH denotes -
T1325 3869-3875 NN denotes length
T1327 3876-3880 NN denotes Atrx
T1323 3881-3888 NN denotes protein
T1328 3889-3891 IN denotes in
T1329 3892-3894 NN denotes ES
T1330 3895-3900 NNS denotes cells
T1331 3901-3904 CC denotes and
T1332 3905-3907 IN denotes in
T1333 3908-3913 NN denotes mouse
T1334 3914-3921 NNS denotes embryos
T1335 3921-3922 . denotes .
T1574 3933-3943 NN denotes Generation
T1575 3944-3946 IN denotes of
T1576 3947-3949 NN denotes ES
T1577 3950-3955 NNS denotes Cells
T1578 3956-3963 VBG denotes Lacking
T1579 3964-3968 JJ denotes Full
T1581 3968-3969 HYPH denotes -
T1580 3969-3975 NN denotes Length
T1582 3976-3980 NN denotes Atrx
T1583 3980-4159 sentence denotes Like the human gene, the mouse Atrx gene is also X-linked, such that a direct disruption of the single Atrx allele in male ES cells would immediately give rise to the null state.
T1584 3981-3985 IN denotes Like
T1586 3986-3989 DT denotes the
T1588 3990-3995 JJ denotes human
T1587 3996-4000 NN denotes gene
T1589 4000-4002 , denotes ,
T1590 4002-4005 DT denotes the
T1592 4006-4011 NN denotes mouse
T1593 4012-4016 NN denotes Atrx
T1591 4017-4021 NN denotes gene
T1585 4022-4024 VBZ denotes is
T1594 4025-4029 RB denotes also
T1595 4030-4031 NN denotes X
T1597 4031-4032 HYPH denotes -
T1596 4032-4038 VBN denotes linked
T1598 4038-4040 , denotes ,
T1599 4040-4044 JJ denotes such
T1601 4045-4049 IN denotes that
T1602 4050-4051 DT denotes a
T1604 4052-4058 JJ denotes direct
T1603 4059-4069 NN denotes disruption
T1605 4070-4072 IN denotes of
T1606 4073-4076 DT denotes the
T1608 4077-4083 JJ denotes single
T1609 4084-4088 NN denotes Atrx
T1607 4089-4095 NN denotes allele
T1610 4096-4098 IN denotes in
T1611 4099-4103 JJ denotes male
T1613 4104-4106 NN denotes ES
T1612 4107-4112 NNS denotes cells
T1614 4113-4118 MD denotes would
T1615 4119-4130 RB denotes immediately
T1600 4131-4135 VB denotes give
T1616 4136-4140 NN denotes rise
T1617 4141-4143 IN denotes to
T1618 4144-4147 DT denotes the
T1620 4148-4152 JJ denotes null
T1619 4153-4158 NN denotes state
T1621 4158-4159 . denotes .
T1622 4159-4326 sentence denotes No targeted clones were recovered after attempted homologous recombination in male E14TG2a ES cells using two different vectors that removed exon 18 of the Atrx gene.
T1623 4160-4162 DT denotes No
T1625 4163-4171 VBN denotes targeted
T1624 4172-4178 NNS denotes clones
T1627 4179-4183 VBD denotes were
T1626 4184-4193 VBN denotes recovered
T1628 4194-4199 IN denotes after
T1629 4200-4209 VBN denotes attempted
T1631 4210-4220 JJ denotes homologous
T1630 4221-4234 NN denotes recombination
T1632 4235-4237 IN denotes in
T1633 4238-4242 JJ denotes male
T1635 4243-4250 NN denotes E14TG2a
T1636 4251-4253 NN denotes ES
T1634 4254-4259 NNS denotes cells
T1637 4260-4265 VBG denotes using
T1638 4266-4269 CD denotes two
T1640 4270-4279 JJ denotes different
T1639 4280-4287 NNS denotes vectors
T1641 4288-4292 WDT denotes that
T1642 4293-4300 VBD denotes removed
T1643 4301-4305 NN denotes exon
T1644 4306-4308 CD denotes 18
T1645 4309-4311 IN denotes of
T1646 4312-4315 DT denotes the
T1648 4316-4320 NN denotes Atrx
T1647 4321-4325 NN denotes gene
T1649 4325-4326 . denotes .
T1650 4326-4569 sentence denotes Exon 18 encodes the first of the seven motifs composing the conserved SNF2-like domain of Atrx (Figure 1); mutation of the corresponding motif of the yeast SNF2 protein has been shown to severely impair SWI/SNF-dependent gene expression [12].
T1651 4327-4331 NN denotes Exon
T1653 4332-4334 CD denotes 18
T1652 4335-4342 VBZ denotes encodes
T1655 4343-4346 DT denotes the
T1656 4347-4352 JJ denotes first
T1657 4353-4355 IN denotes of
T1658 4356-4359 DT denotes the
T1660 4360-4365 CD denotes seven
T1659 4366-4372 NNS denotes motifs
T1661 4373-4382 VBG denotes composing
T1662 4383-4386 DT denotes the
T1664 4387-4396 VBN denotes conserved
T1665 4397-4401 NN denotes SNF2
T1667 4401-4402 HYPH denotes -
T1666 4402-4406 JJ denotes like
T1663 4407-4413 NN denotes domain
T1668 4414-4416 IN denotes of
T1669 4417-4421 NN denotes Atrx
T1670 4422-4423 -LRB- denotes (
T1671 4423-4429 NN denotes Figure
T1672 4430-4431 CD denotes 1
T1673 4431-4432 -RRB- denotes )
T1674 4432-4433 : denotes ;
T1675 4434-4442 NN denotes mutation
T1676 4443-4445 IN denotes of
T1677 4446-4449 DT denotes the
T1679 4450-4463 VBG denotes corresponding
T1678 4464-4469 NN denotes motif
T1680 4470-4472 IN denotes of
T1681 4473-4476 DT denotes the
T1683 4477-4482 NN denotes yeast
T1684 4483-4487 NN denotes SNF2
T1682 4488-4495 NN denotes protein
T1685 4496-4499 VBZ denotes has
T1686 4500-4504 VBN denotes been
T1654 4505-4510 VBN denotes shown
T1687 4511-4513 TO denotes to
T1689 4514-4522 RB denotes severely
T1688 4523-4529 VB denotes impair
T1690 4530-4533 NN denotes SWI
T1692 4533-4534 HYPH denotes /
T1691 4534-4537 NN denotes SNF
T1694 4537-4538 HYPH denotes -
T1693 4538-4547 JJ denotes dependent
T1696 4548-4552 NN denotes gene
T1695 4553-4563 NN denotes expression
T1697 4564-4565 -LRB- denotes [
T1698 4565-4567 CD denotes 12
T1699 4567-4568 -RRB- denotes ]
T1700 4568-4569 . denotes .
T1701 4569-4771 sentence denotes The failure to recover targeted clones with these vectors suggested that Atrx may be important for normal ES cell growth and expansion and that direct targeting of the single locus may not be possible.
T1702 4570-4573 DT denotes The
T1703 4574-4581 NN denotes failure
T1705 4582-4584 TO denotes to
T1706 4585-4592 VB denotes recover
T1707 4593-4601 VBN denotes targeted
T1708 4602-4608 NNS denotes clones
T1709 4609-4613 IN denotes with
T1710 4614-4619 DT denotes these
T1711 4620-4627 NNS denotes vectors
T1704 4628-4637 VBD denotes suggested
T1712 4638-4642 IN denotes that
T1714 4643-4647 NN denotes Atrx
T1715 4648-4651 MD denotes may
T1713 4652-4654 VB denotes be
T1716 4655-4664 JJ denotes important
T1717 4665-4668 IN denotes for
T1718 4669-4675 JJ denotes normal
T1720 4676-4678 NN denotes ES
T1719 4679-4683 NN denotes cell
T1721 4684-4690 NN denotes growth
T1722 4691-4694 CC denotes and
T1723 4695-4704 NN denotes expansion
T1724 4705-4708 CC denotes and
T1725 4709-4713 IN denotes that
T1727 4714-4720 JJ denotes direct
T1728 4721-4730 NN denotes targeting
T1729 4731-4733 IN denotes of
T1730 4734-4737 DT denotes the
T1732 4738-4744 JJ denotes single
T1731 4745-4750 NN denotes locus
T1733 4751-4754 MD denotes may
T1734 4755-4758 RB denotes not
T1726 4759-4761 VB denotes be
T1735 4762-4770 JJ denotes possible
T1736 4770-4771 . denotes .
T1737 4771-5002 sentence denotes We therefore adopted a conditional strategy for targeting exon 18 (Figure 2) and recovered two clones in which exon 18 has been flanked by loxP recognition sites for the Cre recombinase (Atrx flox allele in Figure 2A) (Figure 2B).
T1738 4772-4774 PRP denotes We
T1740 4775-4784 RB denotes therefore
T1739 4785-4792 VBD denotes adopted
T1741 4793-4794 DT denotes a
T1743 4795-4806 JJ denotes conditional
T1742 4807-4815 NN denotes strategy
T1744 4816-4819 IN denotes for
T1745 4820-4829 VBG denotes targeting
T1746 4830-4834 NN denotes exon
T1747 4835-4837 CD denotes 18
T1748 4838-4839 -LRB- denotes (
T1749 4839-4845 NN denotes Figure
T1750 4846-4847 CD denotes 2
T1751 4847-4848 -RRB- denotes )
T1752 4849-4852 CC denotes and
T1753 4853-4862 VBD denotes recovered
T1754 4863-4866 CD denotes two
T1755 4867-4873 NNS denotes clones
T1756 4874-4876 IN denotes in
T1758 4877-4882 WDT denotes which
T1759 4883-4887 NN denotes exon
T1760 4888-4890 CD denotes 18
T1761 4891-4894 VBZ denotes has
T1762 4895-4899 VBN denotes been
T1757 4900-4907 VBN denotes flanked
T1763 4908-4910 IN denotes by
T1764 4911-4915 NN denotes loxP
T1766 4916-4927 NN denotes recognition
T1765 4928-4933 NNS denotes sites
T1767 4934-4937 IN denotes for
T1768 4938-4941 DT denotes the
T1770 4942-4945 NN denotes Cre
T1769 4946-4957 NN denotes recombinase
T1771 4958-4959 -LRB- denotes (
T1773 4959-4968 NN denotes Atrx flox
T1772 4969-4975 NN denotes allele
T1774 4976-4978 IN denotes in
T1775 4979-4985 NN denotes Figure
T1776 4986-4988 NN denotes 2A
T1777 4988-4989 -RRB- denotes )
T1778 4990-4991 -LRB- denotes (
T1780 4991-4997 NN denotes Figure
T1779 4998-5000 NN denotes 2B
T1781 5000-5001 -RRB- denotes )
T1782 5001-5002 . denotes .
T1783 5002-5087 sentence denotes This allele also contains a loxP-flanked MC1-neor cassette in intron 17 (Figure 2A).
T1784 5003-5007 DT denotes This
T1785 5008-5014 NN denotes allele
T1787 5015-5019 RB denotes also
T1786 5020-5028 VBZ denotes contains
T1788 5029-5030 DT denotes a
T1790 5031-5035 NN denotes loxP
T1792 5035-5036 HYPH denotes -
T1791 5036-5043 VBN denotes flanked
T1793 5044-5047 NN denotes MC1
T1795 5047-5048 HYPH denotes -
T1794 5048-5052 NN denotes neor
T1789 5053-5061 NN denotes cassette
T1796 5062-5064 IN denotes in
T1797 5065-5071 NN denotes intron
T1798 5072-5074 CD denotes 17
T1799 5075-5076 -LRB- denotes (
T1801 5076-5082 NN denotes Figure
T1800 5083-5085 NN denotes 2A
T1802 5085-5086 -RRB- denotes )
T1803 5086-5087 . denotes .
T1804 5087-5261 sentence denotes Northern and Western blot analyses (Figure 2D and 2E) confirmed that the Atrx flox clones continued to express both full-length Atrx protein and the truncated Atrxt isoform.
T1805 5088-5096 NNP denotes Northern
T1807 5097-5100 CC denotes and
T1808 5101-5108 NNP denotes Western
T1806 5109-5113 NN denotes blot
T1809 5114-5122 NNS denotes analyses
T1811 5123-5124 -LRB- denotes (
T1813 5124-5130 NN denotes Figure
T1812 5131-5133 NN denotes 2D
T1814 5134-5137 CC denotes and
T1815 5138-5140 NN denotes 2E
T1816 5140-5141 -RRB- denotes )
T1810 5142-5151 VBD denotes confirmed
T1817 5152-5156 IN denotes that
T1819 5157-5160 DT denotes the
T1821 5161-5170 NN denotes Atrx flox
T1820 5171-5177 NNS denotes clones
T1818 5178-5187 VBD denotes continued
T1822 5188-5190 TO denotes to
T1823 5191-5198 VB denotes express
T1824 5199-5203 CC denotes both
T1826 5204-5208 JJ denotes full
T1828 5208-5209 HYPH denotes -
T1827 5209-5215 NN denotes length
T1829 5216-5220 NN denotes Atrx
T1825 5221-5228 NN denotes protein
T1830 5229-5232 CC denotes and
T1831 5233-5236 DT denotes the
T1833 5237-5246 VBN denotes truncated
T1834 5247-5252 NN denotes Atrxt
T1832 5253-5260 NN denotes isoform
T1835 5260-5261 . denotes .
T1836 5261-7993 sentence denotes Figure 2 Cre-Mediated Ablation of Full-Length Atrx Protein in ES Cells (A) Strategy for targeted deletion of exon 18 of the Atrx gene. The top line shows the wild-type allele (Atrx WT) at the region surrounding exon 18. Below is shown the targeting vector and the targeted allele (Atrx flox) resulting from homologous recombination. The loxP target sites of the Cre recombinase are shown as black triangles, and the three possible recombination events that can be mediated by the Cre recombinase are indicated (labelled A, B, and C in the Atrx flox allele). At bottom is shown the Cre-recombined allele (Atrx Δ18Δneo) (resulting from recombination event C) in which both exon 18 and the MC1neopA selection cassette have been deleted. EcoRI (labelled E) and SacI (labelled S) sites present on the targeted 129 strain X chromosome are indicated. Black bars indicate the positions of the probes used in Southern blots. (B) Southern blot analysis of EcoRI-digested DNA from either wild-type ES cells (E14) or targeted ES cell clones bearing the Atrx flox allele (1/F12 and 1/G11) hybridised with either the 20/27 (left blot) or Hae0.9 (right blot) probes. The EcoRI fragment of the Atrx WT allele (18.5 kb) has been replaced with the expected fragments of 11.2 kb (20/27 probe) or 8.5 kb (Hae0.9 probe) (C) Southern blot analysis of SacI-digested DNA from either wild-type ES cells (E14) or targeted ES cell clones bearing the Atrx flox allele (1/F12 and 1/G11) or Cre-recombinant clones derived from these (1/F12B1F12 and 1/G11D5). The membrane was hybridised with the intron 17 probe indicated in (A). The expected bands of 6.2 (Atrx WT), 5.0 (Atrx flox), and 2.8 (Atrx Δ18Δneo) kb were observed. (D) Northern blot analysis of RNA from ES cells shown in (C). The membrane was hybridised first to a probe from exon 10 of the Atrx gene (top blot) and subsequently to a β-actin cDNA probe as loading control (bottom blot). The transcripts responsible for full-length Atrx (~10 kb) and the truncated Atrxt isoforms (~7 kb) are indicated. (E) Western blot analysis of whole-cell extracts from the clones shown in (C) using an anti-ATRX monoclonal antibody (23C, raised against peptide A2 of the human ATRX protein shown in Figure 1). The full-length and truncated Atrx isoforms are indicated. To generate the full deletion in ES cells, the Atrx flox clones (1/F12 and 1/G11) were transiently transfected with a Cre-recombinase expression plasmid (pCAGGS-Cre-IRESpuro), and subclones were recovered bearing an allele (Atrx Δ18Δneo in Figure 2A) in which both exon 18 and the neor cassette had been deleted by the Cre recombinase (resulting from the recombination event labelled “C” in the Atrx flox allele shown in Figure 2A) (Figure 2C).
T1837 7549-7551 TO denotes To
T1838 7552-7560 VB denotes generate
T1840 7561-7564 DT denotes the
T1842 7565-7569 JJ denotes full
T1841 7570-7578 NN denotes deletion
T1843 7579-7581 IN denotes in
T1844 7582-7584 NN denotes ES
T1845 7585-7590 NNS denotes cells
T1846 7590-7592 , denotes ,
T1847 7592-7595 DT denotes the
T1849 7596-7605 NN denotes Atrx flox
T1848 7606-7612 NNS denotes clones
T1850 7613-7614 -LRB- denotes (
T1852 7614-7615 CD denotes 1
T1853 7615-7616 HYPH denotes /
T1851 7616-7619 NN denotes F12
T1854 7620-7623 CC denotes and
T1855 7624-7625 CD denotes 1
T1857 7625-7626 HYPH denotes /
T1856 7626-7629 NN denotes G11
T1858 7629-7630 -RRB- denotes )
T1859 7631-7635 VBD denotes were
T1860 7636-7647 RB denotes transiently
T1839 7648-7659 VBN denotes transfected
T1861 7660-7664 IN denotes with
T1862 7665-7666 DT denotes a
T1864 7667-7670 NN denotes Cre
T1866 7670-7671 HYPH denotes -
T1865 7671-7682 NN denotes recombinase
T1867 7683-7693 NN denotes expression
T1863 7694-7701 NN denotes plasmid
T1868 7702-7703 -LRB- denotes (
T1869 7703-7709 NN denotes pCAGGS
T1871 7709-7710 HYPH denotes -
T1872 7710-7713 NN denotes Cre
T1873 7713-7714 HYPH denotes -
T1870 7714-7722 NN denotes IRESpuro
T1874 7722-7723 -RRB- denotes )
T1875 7723-7725 , denotes ,
T1876 7725-7728 CC denotes and
T1877 7729-7738 NNS denotes subclones
T1879 7739-7743 VBD denotes were
T1880 7744-7753 VBN denotes recovered
T1878 7754-7761 VBG denotes bearing
T1881 7762-7764 DT denotes an
T1882 7765-7771 NN denotes allele
T1883 7772-7773 -LRB- denotes (
T1884 7773-7785 NN denotes Atrx Δ18Δneo
T1885 7786-7788 IN denotes in
T1886 7789-7795 NN denotes Figure
T1887 7796-7798 NN denotes 2A
T1888 7798-7799 -RRB- denotes )
T1889 7800-7802 IN denotes in
T1891 7803-7808 WDT denotes which
T1892 7809-7813 CC denotes both
T1893 7814-7818 NN denotes exon
T1894 7819-7821 CD denotes 18
T1895 7822-7825 CC denotes and
T1896 7826-7829 DT denotes the
T1898 7830-7834 NN denotes neor
T1897 7835-7843 NN denotes cassette
T1899 7844-7847 VBD denotes had
T1900 7848-7852 VBN denotes been
T1890 7853-7860 VBN denotes deleted
T1901 7861-7863 IN denotes by
T1902 7864-7867 DT denotes the
T1904 7868-7871 NN denotes Cre
T1903 7872-7883 NN denotes recombinase
T1905 7884-7885 -LRB- denotes (
T1906 7885-7894 VBG denotes resulting
T1907 7895-7899 IN denotes from
T1908 7900-7903 DT denotes the
T1910 7904-7917 NN denotes recombination
T1909 7918-7923 NN denotes event
T1911 7924-7932 VBN denotes labelled
T1912 7933-7934 `` denotes “
T1913 7934-7935 NN denotes C
T1914 7935-7936 '' denotes ”
T1915 7937-7939 IN denotes in
T1916 7940-7943 DT denotes the
T1918 7944-7953 NN denotes Atrx flox
T1917 7954-7960 NN denotes allele
T1919 7961-7966 VBN denotes shown
T1920 7967-7969 IN denotes in
T1921 7970-7976 NN denotes Figure
T1922 7977-7979 NN denotes 2A
T1923 7979-7980 -RRB- denotes )
T1924 7981-7982 -LRB- denotes (
T1926 7982-7988 NN denotes Figure
T1925 7989-7991 NN denotes 2C
T1927 7991-7992 -RRB- denotes )
T1928 7992-7993 . denotes .
T1929 7993-8274 sentence denotes Northern and Western blot analyses (Figure 2D and 2E) revealed that the full-length Atrx transcript and protein is completely abolished in the Atrx Δ18Δneo recombinant clones, suggesting that deletion of this region has a highly destabilising effect on the full-length transcript.
T1930 7994-8002 NNP denotes Northern
T1932 8003-8006 CC denotes and
T1933 8007-8014 NNP denotes Western
T1931 8015-8019 NN denotes blot
T1934 8020-8028 NNS denotes analyses
T1936 8029-8030 -LRB- denotes (
T1938 8030-8036 NN denotes Figure
T1937 8037-8039 NN denotes 2D
T1939 8040-8043 CC denotes and
T1940 8044-8046 NN denotes 2E
T1941 8046-8047 -RRB- denotes )
T1935 8048-8056 VBD denotes revealed
T1942 8057-8061 IN denotes that
T1944 8062-8065 DT denotes the
T1946 8066-8070 JJ denotes full
T1948 8070-8071 HYPH denotes -
T1947 8071-8077 NN denotes length
T1949 8078-8082 NN denotes Atrx
T1945 8083-8093 NN denotes transcript
T1950 8094-8097 CC denotes and
T1951 8098-8105 NN denotes protein
T1952 8106-8108 VBZ denotes is
T1953 8109-8119 RB denotes completely
T1943 8120-8129 VBN denotes abolished
T1954 8130-8132 IN denotes in
T1955 8133-8136 DT denotes the
T1957 8137-8149 NN denotes Atrx Δ18Δneo
T1958 8150-8161 JJ denotes recombinant
T1956 8162-8168 NNS denotes clones
T1959 8168-8170 , denotes ,
T1960 8170-8180 VBG denotes suggesting
T1961 8181-8185 IN denotes that
T1963 8186-8194 NN denotes deletion
T1964 8195-8197 IN denotes of
T1965 8198-8202 DT denotes this
T1966 8203-8209 NN denotes region
T1962 8210-8213 VBZ denotes has
T1967 8214-8215 DT denotes a
T1969 8216-8222 RB denotes highly
T1970 8223-8236 JJ denotes destabilising
T1968 8237-8243 NN denotes effect
T1971 8244-8246 IN denotes on
T1972 8247-8250 DT denotes the
T1974 8251-8255 JJ denotes full
T1976 8255-8256 HYPH denotes -
T1975 8256-8262 NN denotes length
T1973 8263-8273 NN denotes transcript
T1977 8273-8274 . denotes .
T1978 8274-8430 sentence denotes As expected, the truncated Atrxt isoform, the transcript of which is terminated within intron 11 [4], is unaffected by the deletion of exon 18 (Figure 2E).
T1979 8275-8277 IN denotes As
T1980 8278-8286 VBN denotes expected
T1982 8286-8288 , denotes ,
T1983 8288-8291 DT denotes the
T1985 8292-8301 VBN denotes truncated
T1986 8302-8307 NN denotes Atrxt
T1984 8308-8315 NN denotes isoform
T1987 8315-8317 , denotes ,
T1988 8317-8320 DT denotes the
T1989 8321-8331 NN denotes transcript
T1991 8332-8334 IN denotes of
T1992 8335-8340 WDT denotes which
T1993 8341-8343 VBZ denotes is
T1990 8344-8354 VBN denotes terminated
T1994 8355-8361 IN denotes within
T1995 8362-8368 NN denotes intron
T1996 8369-8371 CD denotes 11
T1997 8372-8373 -LRB- denotes [
T1998 8373-8374 CD denotes 4
T1999 8374-8375 -RRB- denotes ]
T2000 8375-8377 , denotes ,
T1981 8377-8379 VBZ denotes is
T2001 8380-8390 JJ denotes unaffected
T2002 8391-8393 IN denotes by
T2003 8394-8397 DT denotes the
T2004 8398-8406 NN denotes deletion
T2005 8407-8409 IN denotes of
T2006 8410-8414 NN denotes exon
T2007 8415-8417 CD denotes 18
T2008 8418-8419 -LRB- denotes (
T2010 8419-8425 NN denotes Figure
T2009 8426-8428 NN denotes 2E
T2011 8428-8429 -RRB- denotes )
T2012 8429-8430 . denotes .
T2013 8430-8634 sentence denotes While the function of Atrxt is not yet clear, this isoform, which contains the PHD-like domain but not the SWI/SNF motifs (Figure 1), is unlikely to be functionally equivalent to the full-length protein.
T2014 8431-8436 IN denotes While
T2016 8437-8440 DT denotes the
T2017 8441-8449 NN denotes function
T2018 8450-8452 IN denotes of
T2019 8453-8458 NN denotes Atrxt
T2015 8459-8461 VBZ denotes is
T2021 8462-8465 RB denotes not
T2022 8466-8469 RB denotes yet
T2023 8470-8475 JJ denotes clear
T2024 8475-8477 , denotes ,
T2025 8477-8481 DT denotes this
T2026 8482-8489 NN denotes isoform
T2027 8489-8491 , denotes ,
T2028 8491-8496 WDT denotes which
T2029 8497-8505 VBZ denotes contains
T2030 8506-8509 DT denotes the
T2032 8510-8513 NN denotes PHD
T2034 8513-8514 HYPH denotes -
T2033 8514-8518 JJ denotes like
T2031 8519-8525 NN denotes domain
T2035 8526-8529 CC denotes but
T2036 8530-8533 RB denotes not
T2037 8534-8537 DT denotes the
T2039 8538-8541 NN denotes SWI
T2041 8541-8542 HYPH denotes /
T2040 8542-8545 NN denotes SNF
T2038 8546-8552 NNS denotes motifs
T2042 8553-8554 -LRB- denotes (
T2043 8554-8560 NN denotes Figure
T2044 8561-8562 CD denotes 1
T2045 8562-8563 -RRB- denotes )
T2046 8563-8565 , denotes ,
T2020 8565-8567 VBZ denotes is
T2047 8568-8576 JJ denotes unlikely
T2048 8577-8579 TO denotes to
T2049 8580-8582 VB denotes be
T2050 8583-8595 RB denotes functionally
T2051 8596-8606 JJ denotes equivalent
T2052 8607-8609 IN denotes to
T2053 8610-8613 DT denotes the
T2055 8614-8618 JJ denotes full
T2057 8618-8619 HYPH denotes -
T2056 8619-8625 NN denotes length
T2054 8626-8633 NN denotes protein
T2058 8633-8634 . denotes .
T2059 8634-8742 sentence denotes Thus, a conditional knockout strategy allowed the isolation of ES cells that are null for full-length Atrx.
T2060 8635-8639 RB denotes Thus
T2062 8639-8641 , denotes ,
T2063 8641-8642 DT denotes a
T2065 8643-8654 JJ denotes conditional
T2066 8655-8663 NN denotes knockout
T2064 8664-8672 NN denotes strategy
T2061 8673-8680 VBD denotes allowed
T2067 8681-8684 DT denotes the
T2068 8685-8694 NN denotes isolation
T2069 8695-8697 IN denotes of
T2070 8698-8700 NN denotes ES
T2071 8701-8706 NNS denotes cells
T2072 8707-8711 WDT denotes that
T2073 8712-8715 VBP denotes are
T2074 8716-8720 JJ denotes null
T2075 8721-8724 IN denotes for
T2076 8725-8729 JJ denotes full
T2078 8729-8730 HYPH denotes -
T2077 8730-8736 NN denotes length
T2079 8737-8741 NN denotes Atrx
T2080 8741-8742 . denotes .
T2534 8744-8753 VBN denotes Perturbed
T2535 8754-8760 NN denotes Growth
T2536 8761-8764 CC denotes and
T2537 8765-8776 NN denotes Methylation
T2538 8777-8784 NNS denotes Defects
T2539 8785-8787 IN denotes in
T2540 8788-8796 JJ denotes Atrxnull
T2542 8797-8799 NN denotes ES
T2541 8800-8805 NNS denotes Cells
T2543 8805-8979 sentence denotes Atrxnull ES cells could be maintained in culture but were generally slower growing than Atrx+ ES clones, and appeared to undergo higher rates of spontaneous differentiation.
T2544 8806-8814 JJ denotes Atrxnull
T2546 8815-8817 NN denotes ES
T2545 8818-8823 NNS denotes cells
T2548 8824-8829 MD denotes could
T2549 8830-8832 VB denotes be
T2547 8833-8843 VBN denotes maintained
T2550 8844-8846 IN denotes in
T2551 8847-8854 NN denotes culture
T2552 8855-8858 CC denotes but
T2553 8859-8863 VBD denotes were
T2554 8864-8873 RB denotes generally
T2555 8874-8880 RBR denotes slower
T2556 8881-8888 VBG denotes growing
T2557 8889-8893 IN denotes than
T2558 8894-8898 NN denotes Atrx
T2560 8898-8899 SYM denotes +
T2559 8900-8902 NN denotes ES
T2561 8903-8909 NNS denotes clones
T2562 8909-8911 , denotes ,
T2563 8911-8914 CC denotes and
T2564 8915-8923 VBD denotes appeared
T2565 8924-8926 TO denotes to
T2566 8927-8934 VB denotes undergo
T2567 8935-8941 JJR denotes higher
T2568 8942-8947 NNS denotes rates
T2569 8948-8950 IN denotes of
T2570 8951-8962 JJ denotes spontaneous
T2571 8963-8978 NN denotes differentiation
T2572 8978-8979 . denotes .
T2573 8979-9113 sentence denotes We investigated directly the effect of Atrx on ES cell growth by comparing Atrx+ and Atrxnull ES cell clones in competition cultures.
T2574 8980-8982 PRP denotes We
T2575 8983-8995 VBD denotes investigated
T2576 8996-9004 RB denotes directly
T2577 9005-9008 DT denotes the
T2578 9009-9015 NN denotes effect
T2579 9016-9018 IN denotes of
T2580 9019-9023 NN denotes Atrx
T2581 9024-9026 IN denotes on
T2582 9027-9029 NN denotes ES
T2583 9030-9034 NN denotes cell
T2584 9035-9041 NN denotes growth
T2585 9042-9044 IN denotes by
T2586 9045-9054 VBG denotes comparing
T2587 9055-9059 NN denotes Atrx
T2589 9059-9060 SYM denotes +
T2590 9061-9064 CC denotes and
T2591 9065-9073 JJ denotes Atrxnull
T2592 9074-9076 NN denotes ES
T2593 9077-9081 NN denotes cell
T2588 9082-9088 NNS denotes clones
T2594 9089-9091 IN denotes in
T2595 9092-9103 NN denotes competition
T2596 9104-9112 NNS denotes cultures
T2597 9112-9113 . denotes .
T2598 9113-9343 sentence denotes Equal numbers of Atrx+ (bearing either an Atrx WT or an Atrx flox allele) and Atrxnull (bearing an Atrx Δ18Δneo allele) ES cells were inoculated into cultures and the mixed cultures were passaged (1:3 split) every 2 d for 8–10 d.
T2599 9114-9119 JJ denotes Equal
T2600 9120-9127 NNS denotes numbers
T2602 9128-9130 IN denotes of
T2603 9131-9135 NN denotes Atrx
T2605 9135-9136 SYM denotes +
T2606 9137-9138 -LRB- denotes (
T2607 9138-9145 VBG denotes bearing
T2608 9146-9152 CC denotes either
T2610 9153-9155 DT denotes an
T2609 9156-9163 NN denotes Atrx WT
T2611 9164-9166 CC denotes or
T2612 9167-9169 DT denotes an
T2613 9170-9179 NN denotes Atrx flox
T2614 9180-9186 NN denotes allele
T2615 9186-9187 -RRB- denotes )
T2616 9188-9191 CC denotes and
T2617 9192-9200 JJ denotes Atrxnull
T2618 9201-9202 -LRB- denotes (
T2619 9202-9209 VBG denotes bearing
T2620 9210-9212 DT denotes an
T2622 9213-9225 NN denotes Atrx Δ18Δneo
T2621 9226-9232 NN denotes allele
T2623 9232-9233 -RRB- denotes )
T2624 9234-9236 NN denotes ES
T2604 9237-9242 NNS denotes cells
T2625 9243-9247 VBD denotes were
T2601 9248-9258 VBN denotes inoculated
T2626 9259-9263 IN denotes into
T2627 9264-9272 NNS denotes cultures
T2628 9273-9276 CC denotes and
T2629 9277-9280 DT denotes the
T2631 9281-9286 VBN denotes mixed
T2630 9287-9295 NNS denotes cultures
T2633 9296-9300 VBD denotes were
T2632 9301-9309 VBN denotes passaged
T2634 9310-9311 -LRB- denotes (
T2636 9311-9312 CD denotes 1
T2637 9312-9313 SYM denotes :
T2638 9313-9314 CD denotes 3
T2635 9315-9320 NN denotes split
T2639 9320-9321 -RRB- denotes )
T2640 9322-9327 DT denotes every
T2642 9328-9329 CD denotes 2
T2641 9330-9331 NNS denotes d
T2643 9332-9335 IN denotes for
T2644 9336-9337 CD denotes 8
T2646 9337-9338 SYM denotes –
T2645 9338-9340 CD denotes 10
T2647 9341-9342 NNS denotes d
T2648 9342-9343 . denotes .
T2649 9343-9472 sentence denotes The relative abundance of the different alleles in the culture at each time point was analysed by Southern blotting (Figure 3A).
T2650 9344-9347 DT denotes The
T2652 9348-9356 JJ denotes relative
T2651 9357-9366 NN denotes abundance
T2654 9367-9369 IN denotes of
T2655 9370-9373 DT denotes the
T2657 9374-9383 JJ denotes different
T2656 9384-9391 NNS denotes alleles
T2658 9392-9394 IN denotes in
T2659 9395-9398 DT denotes the
T2660 9399-9406 NN denotes culture
T2661 9407-9409 IN denotes at
T2662 9410-9414 DT denotes each
T2664 9415-9419 NN denotes time
T2663 9420-9425 NN denotes point
T2665 9426-9429 VBD denotes was
T2653 9430-9438 VBN denotes analysed
T2666 9439-9441 IN denotes by
T2667 9442-9450 NNP denotes Southern
T2668 9451-9459 NN denotes blotting
T2669 9460-9461 -LRB- denotes (
T2671 9461-9467 NN denotes Figure
T2670 9468-9470 NN denotes 3A
T2672 9470-9471 -RRB- denotes )
T2673 9471-9472 . denotes .
T2674 9472-9603 sentence denotes The clone containing the Atrx Δ18Δneo allele was rapidly outgrown by both Atrx WT ES cells and cells bearing the Atrx flox allele.
T2675 9473-9476 DT denotes The
T2676 9477-9482 NN denotes clone
T2678 9483-9493 VBG denotes containing
T2679 9494-9497 DT denotes the
T2681 9498-9510 NN denotes Atrx Δ18Δneo
T2680 9511-9517 NN denotes allele
T2682 9518-9521 VBD denotes was
T2683 9522-9529 RB denotes rapidly
T2677 9530-9538 VBN denotes outgrown
T2684 9539-9541 IN denotes by
T2685 9542-9546 CC denotes both
T2687 9547-9554 NN denotes Atrx WT
T2688 9555-9557 NN denotes ES
T2686 9558-9563 NNS denotes cells
T2689 9564-9567 CC denotes and
T2690 9568-9573 NNS denotes cells
T2691 9574-9581 VBG denotes bearing
T2692 9582-9585 DT denotes the
T2694 9586-9595 NN denotes Atrx flox
T2693 9596-9602 NN denotes allele
T2695 9602-9603 . denotes .
T2696 9603-9797 sentence denotes In a control competition experiment between different clones bearing functional Atrx alleles (Atrx WT and Atrx flox), both clones continued to be equally represented after 8 d of cocultivation.
T2697 9604-9606 IN denotes In
T2699 9607-9608 DT denotes a
T2701 9609-9616 NN denotes control
T2702 9617-9628 NN denotes competition
T2700 9629-9639 NN denotes experiment
T2703 9640-9647 IN denotes between
T2704 9648-9657 JJ denotes different
T2705 9658-9664 NNS denotes clones
T2706 9665-9672 VBG denotes bearing
T2707 9673-9683 JJ denotes functional
T2709 9684-9688 NN denotes Atrx
T2708 9689-9696 NNS denotes alleles
T2710 9697-9698 -LRB- denotes (
T2711 9698-9705 NN denotes Atrx WT
T2712 9706-9709 CC denotes and
T2713 9710-9719 NN denotes Atrx flox
T2714 9719-9720 -RRB- denotes )
T2715 9720-9722 , denotes ,
T2716 9722-9726 DT denotes both
T2717 9727-9733 NNS denotes clones
T2698 9734-9743 VBD denotes continued
T2718 9744-9746 TO denotes to
T2719 9747-9749 VB denotes be
T2720 9750-9757 RB denotes equally
T2721 9758-9769 JJ denotes represented
T2722 9770-9775 IN denotes after
T2723 9776-9777 CD denotes 8
T2724 9778-9779 NNS denotes d
T2725 9780-9782 IN denotes of
T2726 9783-9796 NN denotes cocultivation
T2727 9796-9797 . denotes .
T2728 9797-10030 sentence denotes Thus, although Atrxnull ES cells could be recovered and maintained in culture by a conditional targeting approach, these cocultivation experiments suggested that the absence of Atrx does negatively impact upon normal ES cell growth.
T2729 9798-9802 RB denotes Thus
T2731 9802-9804 , denotes ,
T2732 9804-9812 IN denotes although
T2734 9813-9821 JJ denotes Atrxnull
T2736 9822-9824 NN denotes ES
T2735 9825-9830 NNS denotes cells
T2737 9831-9836 MD denotes could
T2738 9837-9839 VB denotes be
T2733 9840-9849 VBN denotes recovered
T2739 9850-9853 CC denotes and
T2740 9854-9864 VBN denotes maintained
T2741 9865-9867 IN denotes in
T2742 9868-9875 NN denotes culture
T2743 9876-9878 IN denotes by
T2744 9879-9880 DT denotes a
T2746 9881-9892 JJ denotes conditional
T2747 9893-9902 NN denotes targeting
T2745 9903-9911 NN denotes approach
T2748 9911-9913 , denotes ,
T2749 9913-9918 DT denotes these
T2751 9919-9932 NN denotes cocultivation
T2750 9933-9944 NNS denotes experiments
T2730 9945-9954 VBD denotes suggested
T2752 9955-9959 IN denotes that
T2754 9960-9963 DT denotes the
T2755 9964-9971 NN denotes absence
T2756 9972-9974 IN denotes of
T2757 9975-9979 NN denotes Atrx
T2758 9980-9984 VBZ denotes does
T2759 9985-9995 RB denotes negatively
T2753 9996-10002 VB denotes impact
T2760 10003-10007 IN denotes upon
T2761 10008-10014 JJ denotes normal
T2763 10015-10017 NN denotes ES
T2764 10018-10022 NN denotes cell
T2762 10023-10029 NN denotes growth
T2765 10029-10030 . denotes .
T2766 10030-11896 sentence denotes Figure 3 Growth and Methylation Defects in Atrxnull ES Cells (A) Cultures were inoculated with equivalent numbers of ES cells bearing different Atrx alleles as indicated, and were serially passaged. After the indicated days of coculture, DNA extracted from a sample of cells was analysed by Southern blot to detect the Atrx alleles. DNA was digested with SpeI, and the membrane was hybridised with the 20/27 probe shown in Figure 2A. The expected sizes of the different alleles are indicated. (B) Schematic diagram of the transcribed portion of the mouse rDNA repeat with the 18S, 5.8S, and 28S genes indicated. The positions of the limit-digesting enzymes BamH (labelled B) and EcoRI (labelled E) and the probes (RIB3 and RIB4) used in the Southern blots shown in (C) are indicated. Below are shown the locations of the methylation-sensitive enzymes (SmaI, PvuI, and MluI) whose methylation status has been analysed in the Southern blots shown in (C). (C) DNA from Atrx-positive (Atrx+, bearing either an Atrx WT or Atrx flox allele) or Atrxnull (bearing the Atrx Δ18Δneo allele) ES cells and 7-d embryoid bodies were digested with the enzymes shown and analysed by Southern blotting using the probes indicated. Arrows indicate the fully methylated copies (cut by only the limit-digesting enzyme). Phosphorimager quantitation of the blots are shown below. The y-axis shows the percentage of copies that are undigested by the methylation-sensitive enzyme as a percentage of the total signal from cut and uncut rDNA. Mean values are indicated by horizontal lines, and the significance of the differences between the Atrx-positive and Atrxnull populations are shown for each enzyme. To investigate a possible cell-cycle defect in the absence of Atrx, we analysed the cell cycle distribution of bromodeoxyuridine (BrdU)-pulsed ES cells by flow cytometry (Figure S1A).
T2767 11713-11715 TO denotes To
T2768 11716-11727 VB denotes investigate
T2770 11728-11729 DT denotes a
T2772 11730-11738 JJ denotes possible
T2773 11739-11743 NN denotes cell
T2775 11743-11744 HYPH denotes -
T2774 11744-11749 NN denotes cycle
T2771 11750-11756 NN denotes defect
T2776 11757-11759 IN denotes in
T2777 11760-11763 DT denotes the
T2778 11764-11771 NN denotes absence
T2779 11772-11774 IN denotes of
T2780 11775-11779 NN denotes Atrx
T2781 11779-11781 , denotes ,
T2782 11781-11783 PRP denotes we
T2769 11784-11792 VBD denotes analysed
T2783 11793-11796 DT denotes the
T2785 11797-11801 NN denotes cell
T2786 11802-11807 NN denotes cycle
T2784 11808-11820 NN denotes distribution
T2787 11821-11823 IN denotes of
T2788 11824-11841 NN denotes bromodeoxyuridine
T2790 11842-11843 -LRB- denotes (
T2791 11843-11847 NN denotes BrdU
T2792 11847-11848 -RRB- denotes )
T2793 11848-11849 HYPH denotes -
T2789 11849-11855 VBN denotes pulsed
T2795 11856-11858 NN denotes ES
T2794 11859-11864 NNS denotes cells
T2796 11865-11867 IN denotes by
T2797 11868-11872 NN denotes flow
T2798 11873-11882 NN denotes cytometry
T2799 11883-11884 -LRB- denotes (
T2801 11884-11890 NN denotes Figure
T2800 11891-11894 NN denotes S1A
T2802 11894-11895 -RRB- denotes )
T2803 11895-11896 . denotes .
T2804 11896-12068 sentence denotes Surprisingly, both Atrxnull ES cell clones exhibited a cell cycle profile that was indistinguishable from ES cells bearing a functional Atrx allele (Atrx WT or Atrx flox).
T2805 11897-11909 RB denotes Surprisingly
T2807 11909-11911 , denotes ,
T2808 11911-11915 DT denotes both
T2810 11916-11924 JJ denotes Atrxnull
T2812 11925-11927 NN denotes ES
T2811 11928-11932 NN denotes cell
T2809 11933-11939 NNS denotes clones
T2806 11940-11949 VBD denotes exhibited
T2813 11950-11951 DT denotes a
T2815 11952-11956 NN denotes cell
T2816 11957-11962 NN denotes cycle
T2814 11963-11970 NN denotes profile
T2817 11971-11975 WDT denotes that
T2818 11976-11979 VBD denotes was
T2819 11980-11997 JJ denotes indistinguishable
T2820 11998-12002 IN denotes from
T2821 12003-12005 NN denotes ES
T2822 12006-12011 NNS denotes cells
T2823 12012-12019 VBG denotes bearing
T2824 12020-12021 DT denotes a
T2826 12022-12032 JJ denotes functional
T2827 12033-12037 NN denotes Atrx
T2825 12038-12044 NN denotes allele
T2828 12045-12046 -LRB- denotes (
T2829 12046-12053 NN denotes Atrx WT
T2830 12054-12056 CC denotes or
T2831 12057-12066 NN denotes Atrx flox
T2832 12066-12067 -RRB- denotes )
T2833 12067-12068 . denotes .
T2834 12068-12271 sentence denotes We also specifically quantitated the mitotic index within each population by flow cytometry after staining ES cells for phosphorylated (Ser10) histone H3, a specific marker of mitosis (Figure S1B) [13].
T2835 12069-12071 PRP denotes We
T2837 12072-12076 RB denotes also
T2838 12077-12089 RB denotes specifically
T2836 12090-12101 VBD denotes quantitated
T2839 12102-12105 DT denotes the
T2841 12106-12113 JJ denotes mitotic
T2840 12114-12119 NN denotes index
T2842 12120-12126 IN denotes within
T2843 12127-12131 DT denotes each
T2844 12132-12142 NN denotes population
T2845 12143-12145 IN denotes by
T2846 12146-12150 NN denotes flow
T2847 12151-12160 NN denotes cytometry
T2848 12161-12166 IN denotes after
T2849 12167-12175 VBG denotes staining
T2850 12176-12178 NN denotes ES
T2851 12179-12184 NNS denotes cells
T2852 12185-12188 IN denotes for
T2853 12189-12203 VBN denotes phosphorylated
T2855 12204-12205 -LRB- denotes (
T2856 12205-12210 NN denotes Ser10
T2857 12210-12211 -RRB- denotes )
T2858 12212-12219 NN denotes histone
T2854 12220-12222 NN denotes H3
T2859 12222-12224 , denotes ,
T2860 12224-12225 DT denotes a
T2862 12226-12234 JJ denotes specific
T2861 12235-12241 NN denotes marker
T2863 12242-12244 IN denotes of
T2864 12245-12252 NN denotes mitosis
T2865 12253-12254 -LRB- denotes (
T2867 12254-12260 NN denotes Figure
T2866 12261-12264 NN denotes S1B
T2868 12264-12265 -RRB- denotes )
T2869 12266-12267 -LRB- denotes [
T2870 12267-12269 CD denotes 13
T2871 12269-12270 -RRB- denotes ]
T2872 12270-12271 . denotes .
T2873 12271-12448 sentence denotes Consistent with the normal cell-cycle profile observed above, there was no depletion in the size of the mitotic population in the Atrxnull ES clones, despite their slow growth.
T2874 12272-12282 JJ denotes Consistent
T2876 12283-12287 IN denotes with
T2877 12288-12291 DT denotes the
T2879 12292-12298 JJ denotes normal
T2880 12299-12303 NN denotes cell
T2882 12303-12304 HYPH denotes -
T2881 12304-12309 NN denotes cycle
T2878 12310-12317 NN denotes profile
T2883 12318-12326 VBN denotes observed
T2884 12327-12332 RB denotes above
T2885 12332-12334 , denotes ,
T2886 12334-12339 EX denotes there
T2875 12340-12343 VBD denotes was
T2887 12344-12346 DT denotes no
T2888 12347-12356 NN denotes depletion
T2889 12357-12359 IN denotes in
T2890 12360-12363 DT denotes the
T2891 12364-12368 NN denotes size
T2892 12369-12371 IN denotes of
T2893 12372-12375 DT denotes the
T2895 12376-12383 JJ denotes mitotic
T2894 12384-12394 NN denotes population
T2896 12395-12397 IN denotes in
T2897 12398-12401 DT denotes the
T2899 12402-12410 JJ denotes Atrxnull
T2900 12411-12413 NN denotes ES
T2898 12414-12420 NNS denotes clones
T2901 12420-12422 , denotes ,
T2902 12422-12429 IN denotes despite
T2903 12430-12435 PRP$ denotes their
T2905 12436-12440 JJ denotes slow
T2904 12441-12447 NN denotes growth
T2906 12447-12448 . denotes .
T2907 12448-12726 sentence denotes Finally, we investigated whether the growth defect in the Atrxnull ES cells was due to an up-regulation of apoptosis by staining cells with Annexin V (Figure S2) and found that the proportion of apoptotic cells was not significantly affected by the absence of full-length Atrx.
T2908 12449-12456 RB denotes Finally
T2910 12456-12458 , denotes ,
T2911 12458-12460 PRP denotes we
T2909 12461-12473 VBD denotes investigated
T2912 12474-12481 IN denotes whether
T2914 12482-12485 DT denotes the
T2916 12486-12492 NN denotes growth
T2915 12493-12499 NN denotes defect
T2917 12500-12502 IN denotes in
T2918 12503-12506 DT denotes the
T2920 12507-12515 JJ denotes Atrxnull
T2921 12516-12518 NN denotes ES
T2919 12519-12524 NNS denotes cells
T2913 12525-12528 VBD denotes was
T2922 12529-12532 IN denotes due
T2923 12533-12535 IN denotes to
T2924 12536-12538 DT denotes an
T2926 12539-12541 JJ denotes up
T2927 12541-12542 HYPH denotes -
T2925 12542-12552 NN denotes regulation
T2928 12553-12555 IN denotes of
T2929 12556-12565 NN denotes apoptosis
T2930 12566-12568 IN denotes by
T2931 12569-12577 VBG denotes staining
T2932 12578-12583 NNS denotes cells
T2933 12584-12588 IN denotes with
T2934 12589-12596 NN denotes Annexin
T2935 12597-12598 CD denotes V
T2936 12599-12600 -LRB- denotes (
T2938 12600-12606 NN denotes Figure
T2937 12607-12609 NN denotes S2
T2939 12609-12610 -RRB- denotes )
T2940 12611-12614 CC denotes and
T2941 12615-12620 VBD denotes found
T2942 12621-12625 IN denotes that
T2944 12626-12629 DT denotes the
T2945 12630-12640 NN denotes proportion
T2946 12641-12643 IN denotes of
T2947 12644-12653 JJ denotes apoptotic
T2948 12654-12659 NNS denotes cells
T2949 12660-12663 VBD denotes was
T2950 12664-12667 RB denotes not
T2951 12668-12681 RB denotes significantly
T2943 12682-12690 VBN denotes affected
T2952 12691-12693 IN denotes by
T2953 12694-12697 DT denotes the
T2954 12698-12705 NN denotes absence
T2955 12706-12708 IN denotes of
T2956 12709-12713 JJ denotes full
T2958 12713-12714 HYPH denotes -
T2957 12714-12720 NN denotes length
T2959 12721-12725 NN denotes Atrx
T2960 12725-12726 . denotes .
T2961 12726-12866 sentence denotes Thus, the growth defect observed in ES cells lacking Atrx is not due to a specific cell cycle block or significant induction of cell death.
T2962 12727-12731 RB denotes Thus
T2964 12731-12733 , denotes ,
T2965 12733-12736 DT denotes the
T2967 12737-12743 NN denotes growth
T2966 12744-12750 NN denotes defect
T2968 12751-12759 VBN denotes observed
T2969 12760-12762 IN denotes in
T2970 12763-12765 NN denotes ES
T2971 12766-12771 NNS denotes cells
T2972 12772-12779 VBG denotes lacking
T2973 12780-12784 NN denotes Atrx
T2963 12785-12787 VBZ denotes is
T2974 12788-12791 RB denotes not
T2975 12792-12795 IN denotes due
T2976 12796-12798 IN denotes to
T2977 12799-12800 DT denotes a
T2979 12801-12809 JJ denotes specific
T2980 12810-12814 NN denotes cell
T2981 12815-12820 NN denotes cycle
T2978 12821-12826 NN denotes block
T2982 12827-12829 CC denotes or
T2983 12830-12841 JJ denotes significant
T2984 12842-12851 NN denotes induction
T2985 12852-12854 IN denotes of
T2986 12855-12859 NN denotes cell
T2987 12860-12865 NN denotes death
T2988 12865-12866 . denotes .
T2989 12866-13233 sentence denotes While the cause of the proliferative delay is not yet clear, since Atrxnull ES cells appear to undergo higher rates of spontaneous differentiation (unpublished data), it seems likely that the observed growth defect reflects the spontaneous transition from fast-cycling, undifferentiated ES cells into more slowly cycling, differentiated cell types in these cultures.
T2990 12867-12872 IN denotes While
T2992 12873-12876 DT denotes the
T2993 12877-12882 NN denotes cause
T2994 12883-12885 IN denotes of
T2995 12886-12889 DT denotes the
T2997 12890-12903 JJ denotes proliferative
T2996 12904-12909 NN denotes delay
T2991 12910-12912 VBZ denotes is
T2999 12913-12916 RB denotes not
T3000 12917-12920 RB denotes yet
T3001 12921-12926 JJ denotes clear
T3002 12926-12928 , denotes ,
T3003 12928-12933 IN denotes since
T3005 12934-12942 JJ denotes Atrxnull
T3007 12943-12945 NN denotes ES
T3006 12946-12951 NNS denotes cells
T3004 12952-12958 VBP denotes appear
T3008 12959-12961 TO denotes to
T3009 12962-12969 VB denotes undergo
T3010 12970-12976 JJR denotes higher
T3011 12977-12982 NNS denotes rates
T3012 12983-12985 IN denotes of
T3013 12986-12997 JJ denotes spontaneous
T3014 12998-13013 NN denotes differentiation
T3015 13014-13015 -LRB- denotes (
T3017 13015-13026 JJ denotes unpublished
T3016 13027-13031 NNS denotes data
T3018 13031-13032 -RRB- denotes )
T3019 13032-13034 , denotes ,
T3020 13034-13036 PRP denotes it
T2998 13037-13042 VBZ denotes seems
T3021 13043-13049 JJ denotes likely
T3022 13050-13054 IN denotes that
T3024 13055-13058 DT denotes the
T3026 13059-13067 VBN denotes observed
T3027 13068-13074 NN denotes growth
T3025 13075-13081 NN denotes defect
T3023 13082-13090 VBZ denotes reflects
T3028 13091-13094 DT denotes the
T3030 13095-13106 JJ denotes spontaneous
T3029 13107-13117 NN denotes transition
T3031 13118-13122 IN denotes from
T3032 13123-13127 RB denotes fast
T3034 13127-13128 HYPH denotes -
T3033 13128-13135 VBG denotes cycling
T3036 13135-13137 , denotes ,
T3037 13137-13153 JJ denotes undifferentiated
T3038 13154-13156 NN denotes ES
T3035 13157-13162 NNS denotes cells
T3039 13163-13167 IN denotes into
T3040 13168-13172 RBR denotes more
T3041 13173-13179 RB denotes slowly
T3042 13180-13187 VBG denotes cycling
T3044 13187-13189 , denotes ,
T3045 13189-13203 JJ denotes differentiated
T3046 13204-13208 NN denotes cell
T3043 13209-13214 NNS denotes types
T3047 13215-13217 IN denotes in
T3048 13218-13223 DT denotes these
T3049 13224-13232 NNS denotes cultures
T3050 13232-13233 . denotes .
T3051 13233-13429 sentence denotes It has been shown that disease-causing mutations in the human ATRX gene give rise to changes in the normal pattern of DNA methylation at several repetitive sequences within the human genome [11].
T3052 13234-13236 PRP denotes It
T3054 13237-13240 VBZ denotes has
T3055 13241-13245 VBN denotes been
T3053 13246-13251 VBN denotes shown
T3056 13252-13256 IN denotes that
T3058 13257-13264 NN denotes disease
T3060 13264-13265 HYPH denotes -
T3059 13265-13272 VBG denotes causing
T3061 13273-13282 NNS denotes mutations
T3062 13283-13285 IN denotes in
T3063 13286-13289 DT denotes the
T3065 13290-13295 JJ denotes human
T3066 13296-13300 NN denotes ATRX
T3064 13301-13305 NN denotes gene
T3057 13306-13310 VBP denotes give
T3067 13311-13315 NN denotes rise
T3068 13316-13318 IN denotes to
T3069 13319-13326 NNS denotes changes
T3070 13327-13329 IN denotes in
T3071 13330-13333 DT denotes the
T3073 13334-13340 JJ denotes normal
T3072 13341-13348 NN denotes pattern
T3074 13349-13351 IN denotes of
T3075 13352-13355 NN denotes DNA
T3076 13356-13367 NN denotes methylation
T3077 13368-13370 IN denotes at
T3078 13371-13378 JJ denotes several
T3080 13379-13389 JJ denotes repetitive
T3079 13390-13399 NNS denotes sequences
T3081 13400-13406 IN denotes within
T3082 13407-13410 DT denotes the
T3084 13411-13416 JJ denotes human
T3083 13417-13423 NN denotes genome
T3085 13424-13425 -LRB- denotes [
T3086 13425-13427 CD denotes 11
T3087 13427-13428 -RRB- denotes ]
T3088 13428-13429 . denotes .
T3089 13429-13591 sentence denotes Notably, the transcribed region of the ribosomal DNA (rDNA) repeat was found to be significantly hypomethylated in ATR-X patients relative to normal individuals.
T3090 13430-13437 RB denotes Notably
T3092 13437-13439 , denotes ,
T3093 13439-13442 DT denotes the
T3095 13443-13454 VBN denotes transcribed
T3094 13455-13461 NN denotes region
T3096 13462-13464 IN denotes of
T3097 13465-13468 DT denotes the
T3099 13469-13478 JJ denotes ribosomal
T3100 13479-13482 NN denotes DNA
T3101 13483-13484 -LRB- denotes (
T3102 13484-13488 NN denotes rDNA
T3103 13488-13489 -RRB- denotes )
T3098 13490-13496 NN denotes repeat
T3104 13497-13500 VBD denotes was
T3091 13501-13506 VBN denotes found
T3105 13507-13509 TO denotes to
T3107 13510-13512 VB denotes be
T3108 13513-13526 RB denotes significantly
T3106 13527-13541 VBN denotes hypomethylated
T3109 13542-13544 IN denotes in
T3110 13545-13548 NN denotes ATR
T3112 13548-13549 HYPH denotes -
T3111 13549-13550 NN denotes X
T3113 13551-13559 NNS denotes patients
T3114 13560-13568 JJ denotes relative
T3115 13569-13571 IN denotes to
T3116 13572-13578 JJ denotes normal
T3117 13579-13590 NNS denotes individuals
T3118 13590-13591 . denotes .
T3119 13591-13902 sentence denotes Using methylation-sensitive restriction enzymes, we also observed significant hypomethylation at several sites tested within the mouse rDNA repeats in Atrxnull ES cells and 12-d embryoid bodies relative to ES cells and embryoid bodies bearing a functional Atrx allele (Atrx WT or Atrx flox) (Figure 3B and 3C).
T3120 13592-13597 VBG denotes Using
T3122 13598-13609 NN denotes methylation
T3124 13609-13610 HYPH denotes -
T3123 13610-13619 JJ denotes sensitive
T3126 13620-13631 NN denotes restriction
T3125 13632-13639 NNS denotes enzymes
T3127 13639-13641 , denotes ,
T3128 13641-13643 PRP denotes we
T3129 13644-13648 RB denotes also
T3121 13649-13657 VBD denotes observed
T3130 13658-13669 JJ denotes significant
T3131 13670-13685 NN denotes hypomethylation
T3132 13686-13688 IN denotes at
T3133 13689-13696 JJ denotes several
T3134 13697-13702 NNS denotes sites
T3135 13703-13709 VBN denotes tested
T3136 13710-13716 IN denotes within
T3137 13717-13720 DT denotes the
T3139 13721-13726 NN denotes mouse
T3140 13727-13731 NN denotes rDNA
T3138 13732-13739 NNS denotes repeats
T3141 13740-13742 IN denotes in
T3142 13743-13751 JJ denotes Atrxnull
T3144 13752-13754 NN denotes ES
T3143 13755-13760 NNS denotes cells
T3145 13761-13764 CC denotes and
T3146 13765-13767 CD denotes 12
T3148 13767-13768 HYPH denotes -
T3147 13768-13769 NN denotes d
T3150 13770-13778 JJ denotes embryoid
T3149 13779-13785 NNS denotes bodies
T3151 13786-13794 JJ denotes relative
T3152 13795-13797 IN denotes to
T3153 13798-13800 NN denotes ES
T3154 13801-13806 NNS denotes cells
T3155 13807-13810 CC denotes and
T3156 13811-13819 JJ denotes embryoid
T3157 13820-13826 NNS denotes bodies
T3158 13827-13834 VBG denotes bearing
T3159 13835-13836 DT denotes a
T3161 13837-13847 JJ denotes functional
T3162 13848-13852 NN denotes Atrx
T3160 13853-13859 NN denotes allele
T3163 13860-13861 -LRB- denotes (
T3164 13861-13868 NN denotes Atrx WT
T3165 13869-13871 CC denotes or
T3166 13872-13881 NN denotes Atrx flox
T3167 13881-13882 -RRB- denotes )
T3168 13883-13884 -LRB- denotes (
T3170 13884-13890 NN denotes Figure
T3169 13891-13893 NN denotes 3B
T3171 13894-13897 CC denotes and
T3172 13898-13900 NN denotes 3C
T3173 13900-13901 -RRB- denotes )
T3174 13901-13902 . denotes .
T3175 13902-14133 sentence denotes The observation that rDNA is hypomethylated in the absence of Atrx, even in ES cells, is consistent with the finding that hypomethylation of the human rDNA repeats is detectable from an early developmental stage in ATR-X patients.
T3176 13903-13906 DT denotes The
T3177 13907-13918 NN denotes observation
T3179 13919-13923 IN denotes that
T3181 13924-13928 NN denotes rDNA
T3182 13929-13931 VBZ denotes is
T3180 13932-13946 VBN denotes hypomethylated
T3183 13947-13949 IN denotes in
T3184 13950-13953 DT denotes the
T3185 13954-13961 NN denotes absence
T3186 13962-13964 IN denotes of
T3187 13965-13969 NN denotes Atrx
T3188 13969-13971 , denotes ,
T3189 13971-13975 RB denotes even
T3190 13976-13978 IN denotes in
T3191 13979-13981 NN denotes ES
T3192 13982-13987 NNS denotes cells
T3193 13987-13989 , denotes ,
T3178 13989-13991 VBZ denotes is
T3194 13992-14002 JJ denotes consistent
T3195 14003-14007 IN denotes with
T3196 14008-14011 DT denotes the
T3197 14012-14019 NN denotes finding
T3198 14020-14024 IN denotes that
T3200 14025-14040 NN denotes hypomethylation
T3201 14041-14043 IN denotes of
T3202 14044-14047 DT denotes the
T3204 14048-14053 JJ denotes human
T3205 14054-14058 NN denotes rDNA
T3203 14059-14066 NNS denotes repeats
T3199 14067-14069 VBZ denotes is
T3206 14070-14080 JJ denotes detectable
T3207 14081-14085 IN denotes from
T3208 14086-14088 DT denotes an
T3210 14089-14094 JJ denotes early
T3211 14095-14108 JJ denotes developmental
T3209 14109-14114 NN denotes stage
T3212 14115-14117 IN denotes in
T3213 14118-14121 NN denotes ATR
T3215 14121-14122 HYPH denotes -
T3214 14122-14123 NN denotes X
T3216 14124-14132 NNS denotes patients
T3217 14132-14133 . denotes .
T3218 14133-14462 sentence denotes Other mouse repetitive sequence elements surveyed in ES cell DNA include the heterochromatic major satellite (assayed with MaeII) and minor satellite (assayed with HpaII) repeats, as well as interspersed retroviral repeats of the intracisternal A particle (IAP) type and the Line 1 and Sine B1 families (all assayed with HpaII).
T3219 14134-14139 JJ denotes Other
T3221 14140-14145 NN denotes mouse
T3222 14146-14156 JJ denotes repetitive
T3223 14157-14165 NN denotes sequence
T3220 14166-14174 NNS denotes elements
T3225 14175-14183 VBN denotes surveyed
T3226 14184-14186 IN denotes in
T3227 14187-14189 NN denotes ES
T3229 14190-14194 NN denotes cell
T3228 14195-14198 NN denotes DNA
T3224 14199-14206 VBP denotes include
T3230 14207-14210 DT denotes the
T3232 14211-14226 JJ denotes heterochromatic
T3234 14227-14232 JJ denotes major
T3233 14233-14242 NN denotes satellite
T3235 14243-14244 -LRB- denotes (
T3236 14244-14251 VBN denotes assayed
T3237 14252-14256 IN denotes with
T3238 14257-14262 NN denotes MaeII
T3239 14262-14263 -RRB- denotes )
T3240 14264-14267 CC denotes and
T3241 14268-14273 JJ denotes minor
T3242 14274-14283 NN denotes satellite
T3243 14284-14285 -LRB- denotes (
T3244 14285-14292 VBN denotes assayed
T3245 14293-14297 IN denotes with
T3246 14298-14303 NN denotes HpaII
T3247 14303-14304 -RRB- denotes )
T3231 14305-14312 NNS denotes repeats
T3248 14312-14314 , denotes ,
T3249 14314-14316 RB denotes as
T3251 14317-14321 RB denotes well
T3250 14322-14324 IN denotes as
T3252 14325-14337 VBN denotes interspersed
T3254 14338-14348 JJ denotes retroviral
T3253 14349-14356 NNS denotes repeats
T3255 14357-14359 IN denotes of
T3256 14360-14363 DT denotes the
T3258 14364-14378 JJ denotes intracisternal
T3260 14379-14380 NN denotes A
T3259 14381-14389 NN denotes particle
T3261 14390-14391 -LRB- denotes (
T3262 14391-14394 NN denotes IAP
T3263 14394-14395 -RRB- denotes )
T3257 14396-14400 NN denotes type
T3264 14401-14404 CC denotes and
T3265 14405-14408 DT denotes the
T3267 14409-14413 NN denotes Line
T3268 14414-14415 CD denotes 1
T3269 14416-14419 CC denotes and
T3270 14420-14424 NN denotes Sine
T3271 14425-14427 NN denotes B1
T3266 14428-14436 NNS denotes families
T3272 14437-14438 -LRB- denotes (
T3273 14438-14441 DT denotes all
T3274 14442-14449 VBN denotes assayed
T3275 14450-14454 IN denotes with
T3276 14455-14460 NN denotes HpaII
T3277 14460-14461 -RRB- denotes )
T3278 14461-14462 . denotes .
T3279 14462-14716 sentence denotes These repeats were found to be moderately (Line 1 and Sine B1) or highly (IAP, major satellite, minor satellite) methylated in wild-type ES cells, and this methylation was not detectably perturbed by the absence of Atrx (Figure S3 and unpublished data).
T3280 14463-14468 DT denotes These
T3281 14469-14476 NNS denotes repeats
T3283 14477-14481 VBD denotes were
T3282 14482-14487 VBN denotes found
T3284 14488-14490 TO denotes to
T3285 14491-14493 VB denotes be
T3286 14494-14504 RB denotes moderately
T3288 14505-14506 -LRB- denotes (
T3289 14506-14510 NN denotes Line
T3290 14511-14512 CD denotes 1
T3291 14513-14516 CC denotes and
T3292 14517-14521 NN denotes Sine
T3293 14522-14524 NN denotes B1
T3294 14524-14525 -RRB- denotes )
T3295 14526-14528 CC denotes or
T3296 14529-14535 RB denotes highly
T3297 14536-14537 -LRB- denotes (
T3298 14537-14540 NN denotes IAP
T3299 14540-14542 , denotes ,
T3300 14542-14547 JJ denotes major
T3301 14548-14557 NN denotes satellite
T3302 14557-14559 , denotes ,
T3303 14559-14564 JJ denotes minor
T3304 14565-14574 NN denotes satellite
T3305 14574-14575 -RRB- denotes )
T3287 14576-14586 JJ denotes methylated
T3306 14587-14589 IN denotes in
T3307 14590-14594 JJ denotes wild
T3309 14594-14595 HYPH denotes -
T3308 14595-14599 NN denotes type
T3311 14600-14602 NN denotes ES
T3310 14603-14608 NNS denotes cells
T3312 14608-14610 , denotes ,
T3313 14610-14613 CC denotes and
T3314 14614-14618 DT denotes this
T3315 14619-14630 NN denotes methylation
T3317 14631-14634 VBD denotes was
T3318 14635-14638 RB denotes not
T3319 14639-14649 RB denotes detectably
T3316 14650-14659 VBN denotes perturbed
T3320 14660-14662 IN denotes by
T3321 14663-14666 DT denotes the
T3322 14667-14674 NN denotes absence
T3323 14675-14677 IN denotes of
T3324 14678-14682 NN denotes Atrx
T3325 14683-14684 -LRB- denotes (
T3327 14684-14690 NN denotes Figure
T3326 14691-14693 NN denotes S3
T3328 14694-14697 CC denotes and
T3329 14698-14709 JJ denotes unpublished
T3330 14710-14714 NNS denotes data
T3331 14714-14715 -RRB- denotes )
T3332 14715-14716 . denotes .
T3333 14716-14882 sentence denotes Taken together, these data indicate that the subtle interplay between the ATRX protein and DNA methylation observed in human patients is also present in mouse cells.
T3334 14717-14722 VBN denotes Taken
T3336 14723-14731 RB denotes together
T3337 14731-14733 , denotes ,
T3338 14733-14738 DT denotes these
T3339 14739-14743 NNS denotes data
T3335 14744-14752 VBP denotes indicate
T3340 14753-14757 IN denotes that
T3342 14758-14761 DT denotes the
T3344 14762-14768 JJ denotes subtle
T3343 14769-14778 NN denotes interplay
T3345 14779-14786 IN denotes between
T3346 14787-14790 DT denotes the
T3348 14791-14795 NN denotes ATRX
T3347 14796-14803 NN denotes protein
T3349 14804-14807 CC denotes and
T3350 14808-14811 NN denotes DNA
T3351 14812-14823 NN denotes methylation
T3352 14824-14832 VBN denotes observed
T3353 14833-14835 IN denotes in
T3354 14836-14841 JJ denotes human
T3355 14842-14850 NNS denotes patients
T3341 14851-14853 VBZ denotes is
T3356 14854-14858 RB denotes also
T3357 14859-14866 JJ denotes present
T3358 14867-14869 IN denotes in
T3359 14870-14875 NN denotes mouse
T3360 14876-14881 NNS denotes cells
T3361 14881-14882 . denotes .
T3956 14884-14889 JJ denotes Early
T3958 14890-14899 JJ denotes Embryonic
T3957 14900-14909 NN denotes Lethality
T3959 14910-14912 IN denotes in
T3960 14913-14921 JJ denotes Atrxnull
T3962 14922-14926 JJ denotes Male
T3961 14927-14931 NNS denotes Mice
T3963 14931-15070 sentence denotes To investigate the role of the Atrx protein during mouse development, we initially established lines of mice bearing the Atrx flox allele.
T3964 14932-14934 TO denotes To
T3965 14935-14946 VB denotes investigate
T3967 14947-14950 DT denotes the
T3968 14951-14955 NN denotes role
T3969 14956-14958 IN denotes of
T3970 14959-14962 DT denotes the
T3972 14963-14967 NN denotes Atrx
T3971 14968-14975 NN denotes protein
T3973 14976-14982 IN denotes during
T3974 14983-14988 NN denotes mouse
T3975 14989-15000 NN denotes development
T3976 15000-15002 , denotes ,
T3977 15002-15004 PRP denotes we
T3978 15005-15014 RB denotes initially
T3966 15015-15026 VBD denotes established
T3979 15027-15032 NNS denotes lines
T3980 15033-15035 IN denotes of
T3981 15036-15040 NNS denotes mice
T3982 15041-15048 VBG denotes bearing
T3983 15049-15052 DT denotes the
T3985 15053-15062 NN denotes Atrx flox
T3984 15063-15069 NN denotes allele
T3986 15069-15070 . denotes .
T3987 15070-15269 sentence denotes Two independently targeted Atrx flox ES cell clones with normal male karyotype were injected into C57BL/6 blastocysts to produce chimaeric mice, which were then used to obtain germline transmission.
T3988 15071-15074 CD denotes Two
T3990 15075-15088 RB denotes independently
T3991 15089-15097 VBN denotes targeted
T3992 15098-15107 NN denotes Atrx flox
T3993 15108-15110 NN denotes ES
T3994 15111-15115 NN denotes cell
T3989 15116-15122 NNS denotes clones
T3996 15123-15127 IN denotes with
T3997 15128-15134 JJ denotes normal
T3999 15135-15139 JJ denotes male
T3998 15140-15149 NN denotes karyotype
T4000 15150-15154 VBD denotes were
T3995 15155-15163 VBN denotes injected
T4001 15164-15168 IN denotes into
T4002 15169-15174 NN denotes C57BL
T4004 15174-15175 HYPH denotes /
T4005 15175-15176 CD denotes 6
T4003 15177-15188 NNS denotes blastocysts
T4006 15189-15191 TO denotes to
T4007 15192-15199 VB denotes produce
T4008 15200-15209 JJ denotes chimaeric
T4009 15210-15214 NNS denotes mice
T4010 15214-15216 , denotes ,
T4011 15216-15221 WDT denotes which
T4013 15222-15226 VBD denotes were
T4014 15227-15231 RB denotes then
T4012 15232-15236 VBN denotes used
T4015 15237-15239 TO denotes to
T4016 15240-15246 VB denotes obtain
T4017 15247-15255 NN denotes germline
T4018 15256-15268 NN denotes transmission
T4019 15268-15269 . denotes .
T4020 15269-15454 sentence denotes Intercrosses between males hemizygous (Atrx flox/Y) and females heterozygous (Atrx WT/flox) for the floxed allele were also carried out to generate homozygous females (Atrx flox/flox).
T4021 15270-15282 NNS denotes Intercrosses
T4023 15283-15290 IN denotes between
T4024 15291-15296 NNS denotes males
T4025 15297-15307 JJ denotes hemizygous
T4026 15308-15309 -LRB- denotes (
T4028 15309-15318 NN denotes Atrx flox
T4029 15318-15319 HYPH denotes /
T4027 15319-15320 NN denotes Y
T4030 15320-15321 -RRB- denotes )
T4031 15322-15325 CC denotes and
T4032 15326-15333 NNS denotes females
T4033 15334-15346 JJ denotes heterozygous
T4034 15347-15348 -LRB- denotes (
T4036 15348-15355 NN denotes Atrx WT
T4037 15355-15356 HYPH denotes /
T4035 15356-15360 NN denotes flox
T4038 15360-15361 -RRB- denotes )
T4039 15362-15365 IN denotes for
T4040 15366-15369 DT denotes the
T4042 15370-15376 VBN denotes floxed
T4041 15377-15383 NN denotes allele
T4043 15384-15388 VBD denotes were
T4044 15389-15393 RB denotes also
T4022 15394-15401 VBN denotes carried
T4045 15402-15405 RP denotes out
T4046 15406-15408 TO denotes to
T4047 15409-15417 VB denotes generate
T4048 15418-15428 JJ denotes homozygous
T4049 15429-15436 NNS denotes females
T4050 15437-15438 -LRB- denotes (
T4052 15438-15447 NN denotes Atrx flox
T4053 15447-15448 HYPH denotes /
T4051 15448-15452 NN denotes flox
T4054 15452-15453 -RRB- denotes )
T4055 15453-15454 . denotes .
T4056 15454-15661 sentence denotes Males hemizygous and females heterozygous or homozygous for the Atrx flox allele were viable, appeared healthy, and bred normally, suggesting that, as expected, the Atrx flox allele was functionally normal.
T4057 15455-15460 NNS denotes Males
T4059 15461-15471 JJ denotes hemizygous
T4060 15472-15475 CC denotes and
T4061 15476-15483 NNS denotes females
T4062 15484-15496 JJ denotes heterozygous
T4063 15497-15499 CC denotes or
T4064 15500-15510 JJ denotes homozygous
T4065 15511-15514 IN denotes for
T4066 15515-15518 DT denotes the
T4068 15519-15528 NN denotes Atrx flox
T4067 15529-15535 NN denotes allele
T4058 15536-15540 VBD denotes were
T4069 15541-15547 JJ denotes viable
T4070 15547-15549 , denotes ,
T4071 15549-15557 VBD denotes appeared
T4072 15558-15565 JJ denotes healthy
T4073 15565-15567 , denotes ,
T4074 15567-15570 CC denotes and
T4075 15571-15575 VBD denotes bred
T4076 15576-15584 RB denotes normally
T4077 15584-15586 , denotes ,
T4078 15586-15596 VBG denotes suggesting
T4079 15597-15601 IN denotes that
T4081 15601-15603 , denotes ,
T4082 15603-15605 IN denotes as
T4083 15606-15614 VBN denotes expected
T4084 15614-15616 , denotes ,
T4085 15616-15619 DT denotes the
T4087 15620-15629 NN denotes Atrx flox
T4086 15630-15636 NN denotes allele
T4080 15637-15640 VBD denotes was
T4088 15641-15653 RB denotes functionally
T4089 15654-15660 JJ denotes normal
T4090 15660-15661 . denotes .
T4091 15661-15912 sentence denotes To generate Atrxnull mice by Cre-mediated recombination, the Atrx flox mice were crossed with mice harboring a transgene in which the Cre recombinase is expressed under the control of the regulatory elements of the mouse GATA-1 gene (GATA1-cre) [14].
T4092 15662-15664 TO denotes To
T4093 15665-15673 VB denotes generate
T4095 15674-15682 JJ denotes Atrxnull
T4096 15683-15687 NNS denotes mice
T4097 15688-15690 IN denotes by
T4098 15691-15694 NN denotes Cre
T4100 15694-15695 HYPH denotes -
T4099 15695-15703 VBN denotes mediated
T4101 15704-15717 NN denotes recombination
T4102 15717-15719 , denotes ,
T4103 15719-15722 DT denotes the
T4105 15723-15732 NN denotes Atrx flox
T4104 15733-15737 NNS denotes mice
T4106 15738-15742 VBD denotes were
T4094 15743-15750 VBN denotes crossed
T4107 15751-15755 IN denotes with
T4108 15756-15760 NNS denotes mice
T4109 15761-15770 VBG denotes harboring
T4110 15771-15772 DT denotes a
T4111 15773-15782 NN denotes transgene
T4112 15783-15785 IN denotes in
T4114 15786-15791 WDT denotes which
T4115 15792-15795 DT denotes the
T4117 15796-15799 NN denotes Cre
T4116 15800-15811 NN denotes recombinase
T4118 15812-15814 VBZ denotes is
T4113 15815-15824 VBN denotes expressed
T4119 15825-15830 IN denotes under
T4120 15831-15834 DT denotes the
T4121 15835-15842 NN denotes control
T4122 15843-15845 IN denotes of
T4123 15846-15849 DT denotes the
T4125 15850-15860 JJ denotes regulatory
T4124 15861-15869 NNS denotes elements
T4126 15870-15872 IN denotes of
T4127 15873-15876 DT denotes the
T4129 15877-15882 NN denotes mouse
T4130 15883-15887 NN denotes GATA
T4131 15887-15888 HYPH denotes -
T4132 15888-15889 CD denotes 1
T4128 15890-15894 NN denotes gene
T4133 15895-15896 -LRB- denotes (
T4134 15896-15901 NN denotes GATA1
T4136 15901-15902 HYPH denotes -
T4135 15902-15905 NN denotes cre
T4137 15905-15906 -RRB- denotes )
T4138 15907-15908 -LRB- denotes [
T4139 15908-15910 CD denotes 14
T4140 15910-15911 -RRB- denotes ]
T4141 15911-15912 . denotes .
T4142 15912-16016 sentence denotes Widespread expression of the GATA1-cre transgene has been demonstrated during early embryogenesis [14].
T4143 15913-15923 JJ denotes Widespread
T4144 15924-15934 NN denotes expression
T4146 15935-15937 IN denotes of
T4147 15938-15941 DT denotes the
T4149 15942-15947 NN denotes GATA1
T4151 15947-15948 HYPH denotes -
T4150 15948-15951 NN denotes cre
T4148 15952-15961 NN denotes transgene
T4152 15962-15965 VBZ denotes has
T4153 15966-15970 VBN denotes been
T4145 15971-15983 VBN denotes demonstrated
T4154 15984-15990 IN denotes during
T4155 15991-15996 JJ denotes early
T4156 15997-16010 NN denotes embryogenesis
T4157 16011-16012 -LRB- denotes [
T4158 16012-16014 CD denotes 14
T4159 16014-16015 -RRB- denotes ]
T4160 16015-16016 . denotes .
T4161 16016-16280 sentence denotes We more accurately defined the onset of GATA1-cre expression using a ROSA26 reporter strain, in which a β-galactosidase/neor fusion reporter gene is expressed only after Cre-mediated excision of loxP-flanked transcription and translation termination signals [14].
T4162 16017-16019 PRP denotes We
T4164 16020-16024 RBR denotes more
T4165 16025-16035 RB denotes accurately
T4163 16036-16043 VBN denotes defined
T4166 16044-16047 DT denotes the
T4167 16048-16053 NN denotes onset
T4168 16054-16056 IN denotes of
T4169 16057-16062 NN denotes GATA1
T4171 16062-16063 HYPH denotes -
T4170 16063-16066 NN denotes cre
T4172 16067-16077 NN denotes expression
T4173 16078-16083 VBG denotes using
T4174 16084-16085 DT denotes a
T4176 16086-16092 NN denotes ROSA26
T4177 16093-16101 NN denotes reporter
T4175 16102-16108 NN denotes strain
T4178 16108-16110 , denotes ,
T4179 16110-16112 IN denotes in
T4181 16113-16118 WDT denotes which
T4182 16119-16120 DT denotes a
T4184 16121-16122 NN denotes β
T4186 16122-16123 HYPH denotes -
T4185 16123-16136 NN denotes galactosidase
T4188 16136-16137 HYPH denotes /
T4189 16137-16141 NN denotes neor
T4187 16142-16148 NN denotes fusion
T4190 16149-16157 NN denotes reporter
T4183 16158-16162 NN denotes gene
T4191 16163-16165 VBZ denotes is
T4180 16166-16175 VBN denotes expressed
T4192 16176-16180 RB denotes only
T4193 16181-16186 IN denotes after
T4194 16187-16190 NN denotes Cre
T4196 16190-16191 HYPH denotes -
T4195 16191-16199 VBN denotes mediated
T4197 16200-16208 NN denotes excision
T4198 16209-16211 IN denotes of
T4199 16212-16216 NN denotes loxP
T4201 16216-16217 HYPH denotes -
T4200 16217-16224 VBN denotes flanked
T4202 16225-16238 NN denotes transcription
T4203 16239-16242 CC denotes and
T4204 16243-16254 NN denotes translation
T4205 16255-16266 NN denotes termination
T4206 16267-16274 NNS denotes signals
T4207 16275-16276 -LRB- denotes [
T4208 16276-16278 CD denotes 14
T4209 16278-16279 -RRB- denotes ]
T4210 16279-16280 . denotes .
T4211 16280-16421 sentence denotes We found that the GATA1-cre transgene was already active at the 16-cell morula stage of development (0.5 days postcoitus [dpc]) (Figure 4A).
T4212 16281-16283 PRP denotes We
T4213 16284-16289 VBD denotes found
T4214 16290-16294 IN denotes that
T4216 16295-16298 DT denotes the
T4218 16299-16304 NN denotes GATA1
T4220 16304-16305 HYPH denotes -
T4219 16305-16308 NN denotes cre
T4217 16309-16318 NN denotes transgene
T4215 16319-16322 VBD denotes was
T4221 16323-16330 RB denotes already
T4222 16331-16337 JJ denotes active
T4223 16338-16340 IN denotes at
T4224 16341-16344 DT denotes the
T4226 16345-16347 CD denotes 16
T4228 16347-16348 HYPH denotes -
T4227 16348-16352 NN denotes cell
T4229 16353-16359 NN denotes morula
T4225 16360-16365 NN denotes stage
T4230 16366-16368 IN denotes of
T4231 16369-16380 NN denotes development
T4232 16381-16382 -LRB- denotes (
T4233 16382-16385 CD denotes 0.5
T4234 16386-16390 NNS denotes days
T4235 16391-16401 RB denotes postcoitus
T4236 16402-16403 -LRB- denotes [
T4237 16403-16406 NN denotes dpc
T4238 16406-16407 -RRB- denotes ]
T4239 16407-16408 -RRB- denotes )
T4240 16409-16410 -LRB- denotes (
T4242 16410-16416 NN denotes Figure
T4241 16417-16419 NN denotes 4A
T4243 16419-16420 -RRB- denotes )
T4244 16420-16421 . denotes .
T4245 16421-17593 sentence denotes Figure 4 Timing of Onset of GATA1-Cre Expression and PCR Genotyping of Atrx Alleles (A) GATA1-cre +/+ transgenic males were crossed to females of the ROSA26 reporter strain (ROSA26 +/−), and embryos were recovered at 0.5 dpc (~16-cell morula stage) and stained with X-gal. Cre-mediated activation of the ROSA26 β-galactosidase reporter allele was detected in all cells in embryos in which both alleles are coinherited. (B) Top gel: PCR genotyping of Atrx alleles in embryos using primers PPS1.15 (exon 17) and Mxnp30 (exon 20) as described in Protocol S1. The sizes of PCR products from the different alleles are indicated. Both the Atrx Δ18 (resulting from recombination event B in Figure 2A) and the Atrx Δ18Δneo allele (resulting from recombination event C in Figure 2A) are null for full-length Atrx protein. The bottom gel shows products from a PCR reaction (primers DG52/DG53) used to sex embryos as described in Protocol S1. A 450-bp PCR product is amplified from a mouse Y chromosome-specific satellite repeat. To generate Atrxnull mice, heterozygous floxed females (Atrx WT/flox) were mated with homozygous GATA1-cre transgenic males (Atrx WT/Y;GATA1-cre +/+ ).
T4246 17442-17444 TO denotes To
T4247 17445-17453 VB denotes generate
T4249 17454-17462 JJ denotes Atrxnull
T4250 17463-17467 NNS denotes mice
T4251 17467-17469 , denotes ,
T4252 17469-17481 JJ denotes heterozygous
T4254 17482-17488 VBN denotes floxed
T4253 17489-17496 NNS denotes females
T4255 17497-17498 -LRB- denotes (
T4257 17498-17505 NN denotes Atrx WT
T4258 17505-17506 HYPH denotes /
T4256 17506-17510 NN denotes flox
T4259 17510-17511 -RRB- denotes )
T4260 17512-17516 VBD denotes were
T4248 17517-17522 VBN denotes mated
T4261 17523-17527 IN denotes with
T4262 17528-17538 JJ denotes homozygous
T4264 17539-17544 NN denotes GATA1
T4266 17544-17545 HYPH denotes -
T4265 17545-17548 NN denotes cre
T4267 17549-17559 JJ denotes transgenic
T4263 17560-17565 NNS denotes males
T4268 17566-17567 -LRB- denotes (
T4270 17567-17574 NN denotes Atrx WT
T4272 17574-17575 HYPH denotes /
T4271 17575-17576 NN denotes Y
T4273 17576-17577 : denotes ;
T4274 17577-17582 NN denotes GATA1
T4275 17582-17583 HYPH denotes -
T4269 17583-17586 NN denotes cre
T4276 17587-17588 SYM denotes +
T4277 17588-17589 HYPH denotes /
T4278 17589-17590 SYM denotes +
T4279 17591-17592 -RRB- denotes )
T4280 17592-17593 . denotes .
T4281 17593-17733 sentence denotes No Atrxnull males (Atrx null/Y;GATA1-cre +/− ) were recovered at birth, indicating that the absence of Atrx results in embryonic lethality.
T4282 17594-17596 DT denotes No
T4284 17597-17605 JJ denotes Atrxnull
T4283 17606-17611 NNS denotes males
T4286 17612-17613 -LRB- denotes (
T4288 17613-17622 NN denotes Atrx null
T4290 17622-17623 HYPH denotes /
T4289 17623-17624 NN denotes Y
T4291 17624-17625 : denotes ;
T4292 17625-17630 NN denotes GATA1
T4293 17630-17631 HYPH denotes -
T4287 17631-17634 NN denotes cre
T4294 17635-17636 SYM denotes +
T4295 17636-17637 HYPH denotes /
T4296 17637-17638 SYM denotes −
T4297 17639-17640 -RRB- denotes )
T4298 17641-17645 VBD denotes were
T4285 17646-17655 VBN denotes recovered
T4299 17656-17658 IN denotes at
T4300 17659-17664 NN denotes birth
T4301 17664-17666 , denotes ,
T4302 17666-17676 VBG denotes indicating
T4303 17677-17681 IN denotes that
T4305 17682-17685 DT denotes the
T4306 17686-17693 NN denotes absence
T4307 17694-17696 IN denotes of
T4308 17697-17701 NN denotes Atrx
T4304 17702-17709 VBZ denotes results
T4309 17710-17712 IN denotes in
T4310 17713-17722 JJ denotes embryonic
T4311 17723-17732 NN denotes lethality
T4312 17732-17733 . denotes .
T4313 17733-17836 sentence denotes This finding was unexpected, since human ATR-X patients clearly survive to adulthood (see Discussion).
T4314 17734-17738 DT denotes This
T4315 17739-17746 NN denotes finding
T4316 17747-17750 VBD denotes was
T4317 17751-17761 JJ denotes unexpected
T4318 17761-17763 , denotes ,
T4319 17763-17768 IN denotes since
T4321 17769-17774 JJ denotes human
T4323 17775-17778 NN denotes ATR
T4325 17778-17779 HYPH denotes -
T4324 17779-17780 NN denotes X
T4322 17781-17789 NNS denotes patients
T4326 17790-17797 RB denotes clearly
T4320 17798-17805 VBP denotes survive
T4327 17806-17808 IN denotes to
T4328 17809-17818 NN denotes adulthood
T4329 17819-17820 -LRB- denotes (
T4330 17820-17823 VB denotes see
T4331 17824-17834 NN denotes Discussion
T4332 17834-17835 -RRB- denotes )
T4333 17835-17836 . denotes .
T4334 17836-17990 sentence denotes Embryos were dissected at 7.5, 8.5, and 9.5 dpc and genotyped by PCR analysis of DNA extracted from yolk sac or total embryo (Figure 4B and Protocol S1).
T4335 17837-17844 NNS denotes Embryos
T4337 17845-17849 VBD denotes were
T4336 17850-17859 VBN denotes dissected
T4338 17860-17862 IN denotes at
T4339 17863-17866 CD denotes 7.5
T4341 17866-17868 , denotes ,
T4342 17868-17871 CD denotes 8.5
T4343 17871-17873 , denotes ,
T4344 17873-17876 CC denotes and
T4345 17877-17880 CD denotes 9.5
T4340 17881-17884 NN denotes dpc
T4346 17885-17888 CC denotes and
T4347 17889-17898 VBN denotes genotyped
T4348 17899-17901 IN denotes by
T4349 17902-17905 NN denotes PCR
T4350 17906-17914 NN denotes analysis
T4351 17915-17917 IN denotes of
T4352 17918-17921 NN denotes DNA
T4353 17922-17931 VBN denotes extracted
T4354 17932-17936 IN denotes from
T4355 17937-17941 NN denotes yolk
T4356 17942-17945 NN denotes sac
T4357 17946-17948 CC denotes or
T4358 17949-17954 JJ denotes total
T4359 17955-17961 NN denotes embryo
T4360 17962-17963 -LRB- denotes (
T4362 17963-17969 NN denotes Figure
T4361 17970-17972 NN denotes 4B
T4363 17973-17976 CC denotes and
T4364 17977-17985 NN denotes Protocol
T4365 17986-17988 NN denotes S1
T4366 17988-17989 -RRB- denotes )
T4367 17989-17990 . denotes .
T4368 17990-18093 sentence denotes Atrxnull males were present at expected mendelian ratios (~25%) at both 7.5 dpc and 8.5 dpc (Table 1).
T4369 17991-17999 JJ denotes Atrxnull
T4370 18000-18005 NNS denotes males
T4371 18006-18010 VBD denotes were
T4372 18011-18018 JJ denotes present
T4373 18019-18021 IN denotes at
T4374 18022-18030 VBN denotes expected
T4376 18031-18040 JJ denotes mendelian
T4375 18041-18047 NNS denotes ratios
T4377 18048-18049 -LRB- denotes (
T4379 18049-18050 SYM denotes ~
T4380 18050-18052 CD denotes 25
T4378 18052-18053 NN denotes %
T4381 18053-18054 -RRB- denotes )
T4382 18055-18057 IN denotes at
T4383 18058-18062 CC denotes both
T4385 18063-18066 CD denotes 7.5
T4384 18067-18070 NN denotes dpc
T4386 18071-18074 CC denotes and
T4387 18075-18078 CD denotes 8.5
T4388 18079-18082 NN denotes dpc
T4389 18083-18084 -LRB- denotes (
T4390 18084-18089 NN denotes Table
T4391 18090-18091 CD denotes 1
T4392 18091-18092 -RRB- denotes )
T4393 18092-18093 . denotes .
T4394 18093-18229 sentence denotes However, by 9.5 dpc, depletion was observed both in the number of Atrxnull males (7%) and in the total number of males recovered (31%).
T4395 18094-18101 RB denotes However
T4397 18101-18103 , denotes ,
T4398 18103-18105 IN denotes by
T4399 18106-18109 CD denotes 9.5
T4400 18110-18113 NN denotes dpc
T4401 18113-18115 , denotes ,
T4402 18115-18124 NN denotes depletion
T4403 18125-18128 VBD denotes was
T4396 18129-18137 VBN denotes observed
T4404 18138-18142 CC denotes both
T4405 18143-18145 IN denotes in
T4406 18146-18149 DT denotes the
T4407 18150-18156 NN denotes number
T4408 18157-18159 IN denotes of
T4409 18160-18168 JJ denotes Atrxnull
T4410 18169-18174 NNS denotes males
T4411 18175-18176 -LRB- denotes (
T4413 18176-18177 CD denotes 7
T4412 18177-18178 NN denotes %
T4414 18178-18179 -RRB- denotes )
T4415 18180-18183 CC denotes and
T4416 18184-18186 IN denotes in
T4417 18187-18190 DT denotes the
T4419 18191-18196 JJ denotes total
T4418 18197-18203 NN denotes number
T4420 18204-18206 IN denotes of
T4421 18207-18212 NNS denotes males
T4422 18213-18222 VBN denotes recovered
T4423 18223-18224 -LRB- denotes (
T4425 18224-18226 CD denotes 31
T4424 18226-18227 NN denotes %
T4426 18227-18228 -RRB- denotes )
T4427 18228-18229 . denotes .
T4428 18229-18277 sentence denotes No Atrxnull males were recovered after 9.5 dpc.
T4429 18230-18232 DT denotes No
T4431 18233-18241 JJ denotes Atrxnull
T4430 18242-18247 NNS denotes males
T4433 18248-18252 VBD denotes were
T4432 18253-18262 VBN denotes recovered
T4434 18263-18268 IN denotes after
T4435 18269-18272 CD denotes 9.5
T4436 18273-18276 NN denotes dpc
T4437 18276-18277 . denotes .
T4438 18277-18360 sentence denotes Thus the absence of Atrx gives rise to embryonic lethality in mice before 9.5 dpc.
T4439 18278-18282 RB denotes Thus
T4441 18283-18286 DT denotes the
T4442 18287-18294 NN denotes absence
T4443 18295-18297 IN denotes of
T4444 18298-18302 NN denotes Atrx
T4440 18303-18308 VBZ denotes gives
T4445 18309-18313 NN denotes rise
T4446 18314-18316 IN denotes to
T4447 18317-18326 JJ denotes embryonic
T4448 18327-18336 NN denotes lethality
T4449 18337-18339 IN denotes in
T4450 18340-18344 NNS denotes mice
T4451 18345-18351 IN denotes before
T4452 18352-18355 CD denotes 9.5
T4453 18356-18359 NN denotes dpc
T4454 18359-18360 . denotes .
T4455 18360-18680 sentence denotes Table 1 Distribution of Atrx Genotypes in Timed Matings To investigate the morphology of Atrxnull embryos prior to death, embryos from the above crosses were initially dissected in their deciduas at 7.5 dpc, and paraffin sections were stained with haematoxylin (Figure 5A) or with an anti-ATRX antibody (Figure 5B–5E).
T4456 18361-18420 TO denotes Table 1 Distribution of Atrx Genotypes in Timed Matings To
T4457 18421-18432 VB denotes investigate
T4459 18433-18436 DT denotes the
T4460 18437-18447 NN denotes morphology
T4461 18448-18450 IN denotes of
T4462 18451-18459 JJ denotes Atrxnull
T4463 18460-18467 NNS denotes embryos
T4464 18468-18473 RB denotes prior
T4465 18474-18476 IN denotes to
T4466 18477-18482 NN denotes death
T4467 18482-18484 , denotes ,
T4468 18484-18491 NNS denotes embryos
T4469 18492-18496 IN denotes from
T4470 18497-18500 DT denotes the
T4472 18501-18506 JJ denotes above
T4471 18507-18514 NNS denotes crosses
T4473 18515-18519 VBD denotes were
T4474 18520-18529 RB denotes initially
T4458 18530-18539 VBN denotes dissected
T4475 18540-18542 IN denotes in
T4476 18543-18548 PRP$ denotes their
T4477 18549-18557 NNS denotes deciduas
T4478 18558-18560 IN denotes at
T4479 18561-18564 CD denotes 7.5
T4480 18565-18568 NN denotes dpc
T4481 18568-18570 , denotes ,
T4482 18570-18573 CC denotes and
T4483 18574-18582 NN denotes paraffin
T4484 18583-18591 NNS denotes sections
T4486 18592-18596 VBD denotes were
T4485 18597-18604 VBN denotes stained
T4487 18605-18609 IN denotes with
T4488 18610-18622 NN denotes haematoxylin
T4489 18623-18624 -LRB- denotes (
T4491 18624-18630 NN denotes Figure
T4490 18631-18633 NN denotes 5A
T4492 18633-18634 -RRB- denotes )
T4493 18635-18637 CC denotes or
T4494 18638-18642 IN denotes with
T4495 18643-18645 DT denotes an
T4497 18646-18655 JJ denotes anti-ATRX
T4496 18656-18664 NN denotes antibody
T4498 18665-18666 -LRB- denotes (
T4500 18666-18672 NN denotes Figure
T4499 18673-18675 NN denotes 5B
T4501 18675-18676 SYM denotes –
T4502 18676-18678 NN denotes 5E
T4503 18678-18679 -RRB- denotes )
T4504 18679-18680 . denotes .
T4505 18680-18791 sentence denotes Immunohistochemical staining revealed that Atrx was widely expressed in wild-type 7.5 dpc embryos (Figure 5B).
T4506 18681-18700 JJ denotes Immunohistochemical
T4507 18701-18709 NN denotes staining
T4508 18710-18718 VBD denotes revealed
T4509 18719-18723 IN denotes that
T4511 18724-18728 NN denotes Atrx
T4512 18729-18732 VBD denotes was
T4513 18733-18739 RB denotes widely
T4510 18740-18749 VBN denotes expressed
T4514 18750-18752 IN denotes in
T4515 18753-18757 JJ denotes wild
T4517 18757-18758 HYPH denotes -
T4516 18758-18762 NN denotes type
T4519 18763-18766 CD denotes 7.5
T4520 18767-18770 NN denotes dpc
T4518 18771-18778 NNS denotes embryos
T4521 18779-18780 -LRB- denotes (
T4523 18780-18786 NN denotes Figure
T4522 18787-18789 NN denotes 5B
T4524 18789-18790 -RRB- denotes )
T4525 18790-18791 . denotes .
T4526 18791-18879 sentence denotes Expression was highest in the embryonic region (Figure 5C) and the chorion (Figure 5D).
T4527 18792-18802 NN denotes Expression
T4528 18803-18806 VBD denotes was
T4529 18807-18814 JJS denotes highest
T4530 18815-18817 IN denotes in
T4531 18818-18821 DT denotes the
T4533 18822-18831 JJ denotes embryonic
T4532 18832-18838 NN denotes region
T4534 18839-18840 -LRB- denotes (
T4536 18840-18846 NN denotes Figure
T4535 18847-18849 NN denotes 5C
T4537 18849-18850 -RRB- denotes )
T4538 18851-18854 CC denotes and
T4539 18855-18858 DT denotes the
T4540 18859-18866 NN denotes chorion
T4541 18867-18868 -LRB- denotes (
T4543 18868-18874 NN denotes Figure
T4542 18875-18877 NN denotes 5D
T4544 18877-18878 -RRB- denotes )
T4545 18878-18879 . denotes .
T4546 18879-19006 sentence denotes Detectable but lower levels of expression were observed in the ectoplacental cone (Figure 5D) and surrounding decidual tissue.
T4547 18880-18890 JJ denotes Detectable
T4549 18891-18894 CC denotes but
T4550 18895-18900 JJR denotes lower
T4548 18901-18907 NNS denotes levels
T4552 18908-18910 IN denotes of
T4553 18911-18921 NN denotes expression
T4554 18922-18926 VBD denotes were
T4551 18927-18935 VBN denotes observed
T4555 18936-18938 IN denotes in
T4556 18939-18942 DT denotes the
T4558 18943-18956 JJ denotes ectoplacental
T4557 18957-18961 NN denotes cone
T4559 18962-18963 -LRB- denotes (
T4561 18963-18969 NN denotes Figure
T4560 18970-18972 NN denotes 5D
T4562 18972-18973 -RRB- denotes )
T4563 18974-18977 CC denotes and
T4564 18978-18989 VBG denotes surrounding
T4566 18990-18998 JJ denotes decidual
T4565 18999-19005 NN denotes tissue
T4567 19005-19006 . denotes .
T4568 19006-19142 sentence denotes We also observed very high levels of Atrx expression in trophoblast giant cells (TGCs) surrounding the Reichert's membrane (Figure 5E).
T4569 19007-19009 PRP denotes We
T4571 19010-19014 RB denotes also
T4570 19015-19023 VBD denotes observed
T4572 19024-19028 RB denotes very
T4573 19029-19033 JJ denotes high
T4574 19034-19040 NNS denotes levels
T4575 19041-19043 IN denotes of
T4576 19044-19048 NN denotes Atrx
T4577 19049-19059 NN denotes expression
T4578 19060-19062 IN denotes in
T4579 19063-19074 NN denotes trophoblast
T4581 19075-19080 JJ denotes giant
T4580 19081-19086 NNS denotes cells
T4582 19087-19088 -LRB- denotes (
T4583 19088-19092 NNS denotes TGCs
T4584 19092-19093 -RRB- denotes )
T4585 19094-19105 VBG denotes surrounding
T4586 19106-19109 DT denotes the
T4588 19110-19118 NN denotes Reichert
T4589 19118-19120 POS denotes 's
T4587 19121-19129 NN denotes membrane
T4590 19130-19131 -LRB- denotes (
T4592 19131-19137 NN denotes Figure
T4591 19138-19140 NN denotes 5E
T4593 19140-19141 -RRB- denotes )
T4594 19141-19142 . denotes .
T4595 19142-19297 sentence denotes Within the large nuclei of these TGCs, the typical nuclear association of Atrx with blocks of pericentromeric heterochromatin [15] was clearly observable.
T4596 19143-19149 IN denotes Within
T4598 19150-19153 DT denotes the
T4600 19154-19159 JJ denotes large
T4599 19160-19166 NNS denotes nuclei
T4601 19167-19169 IN denotes of
T4602 19170-19175 DT denotes these
T4603 19176-19180 NNS denotes TGCs
T4604 19180-19182 , denotes ,
T4605 19182-19185 DT denotes the
T4607 19186-19193 JJ denotes typical
T4608 19194-19201 JJ denotes nuclear
T4606 19202-19213 NN denotes association
T4609 19214-19216 IN denotes of
T4610 19217-19221 NN denotes Atrx
T4611 19222-19226 IN denotes with
T4612 19227-19233 NNS denotes blocks
T4613 19234-19236 IN denotes of
T4614 19237-19252 JJ denotes pericentromeric
T4615 19253-19268 NN denotes heterochromatin
T4616 19269-19270 -LRB- denotes [
T4617 19270-19272 CD denotes 15
T4618 19272-19273 -RRB- denotes ]
T4597 19274-19277 VBD denotes was
T4619 19278-19285 RB denotes clearly
T4620 19286-19296 JJ denotes observable
T4621 19296-19297 . denotes .
T4622 19297-19542 sentence denotes Only background staining was seen in the corresponding Atrxnull embryonic tissues (Figure 5B–5D), while expression in the surrounding decidual tissue (of maternal origin) was normal and served as an antibody staining control (unpublished data).
T4623 19298-19302 RB denotes Only
T4625 19303-19313 NN denotes background
T4624 19314-19322 NN denotes staining
T4627 19323-19326 VBD denotes was
T4626 19327-19331 VBN denotes seen
T4628 19332-19334 IN denotes in
T4629 19335-19338 DT denotes the
T4631 19339-19352 VBG denotes corresponding
T4632 19353-19361 JJ denotes Atrxnull
T4633 19362-19371 JJ denotes embryonic
T4630 19372-19379 NNS denotes tissues
T4634 19380-19381 -LRB- denotes (
T4636 19381-19387 NN denotes Figure
T4635 19388-19390 NN denotes 5B
T4637 19390-19391 SYM denotes –
T4638 19391-19393 NN denotes 5D
T4639 19393-19394 -RRB- denotes )
T4640 19394-19396 , denotes ,
T4641 19396-19401 IN denotes while
T4643 19402-19412 NN denotes expression
T4644 19413-19415 IN denotes in
T4645 19416-19419 DT denotes the
T4647 19420-19431 VBG denotes surrounding
T4648 19432-19440 JJ denotes decidual
T4646 19441-19447 NN denotes tissue
T4649 19448-19449 -LRB- denotes (
T4650 19449-19451 IN denotes of
T4651 19452-19460 JJ denotes maternal
T4652 19461-19467 NN denotes origin
T4653 19467-19468 -RRB- denotes )
T4642 19469-19472 VBD denotes was
T4654 19473-19479 JJ denotes normal
T4655 19480-19483 CC denotes and
T4656 19484-19490 VBD denotes served
T4657 19491-19493 IN denotes as
T4658 19494-19496 DT denotes an
T4660 19497-19505 NN denotes antibody
T4661 19506-19514 NN denotes staining
T4659 19515-19522 NN denotes control
T4662 19523-19524 -LRB- denotes (
T4664 19524-19535 JJ denotes unpublished
T4663 19536-19540 NNS denotes data
T4665 19540-19541 -RRB- denotes )
T4666 19541-19542 . denotes .
T4667 19542-19720 sentence denotes Morphologically, 7.5 dpc Atrxnull embryos were dramatically reduced in size and appeared developmentally retarded relative to stage-matched wild-type embryos (Figure 5A and 5B).
T4668 19543-19558 RB denotes Morphologically
T4670 19558-19560 , denotes ,
T4671 19560-19563 CD denotes 7.5
T4672 19564-19567 NNS denotes dpc
T4674 19568-19576 JJ denotes Atrxnull
T4673 19577-19584 NNS denotes embryos
T4675 19585-19589 VBD denotes were
T4676 19590-19602 RB denotes dramatically
T4669 19603-19610 VBN denotes reduced
T4677 19611-19613 IN denotes in
T4678 19614-19618 NN denotes size
T4679 19619-19622 CC denotes and
T4680 19623-19631 VBD denotes appeared
T4681 19632-19647 RB denotes developmentally
T4682 19648-19656 JJ denotes retarded
T4683 19657-19665 JJ denotes relative
T4684 19666-19668 IN denotes to
T4685 19669-19674 NN denotes stage
T4687 19674-19675 HYPH denotes -
T4686 19675-19682 VBN denotes matched
T4689 19683-19687 JJ denotes wild
T4691 19687-19688 HYPH denotes -
T4690 19688-19692 NN denotes type
T4688 19693-19700 NNS denotes embryos
T4692 19701-19702 -LRB- denotes (
T4694 19702-19708 NN denotes Figure
T4693 19709-19711 NN denotes 5A
T4695 19712-19715 CC denotes and
T4696 19716-19718 NN denotes 5B
T4697 19718-19719 -RRB- denotes )
T4698 19719-19720 . denotes .
T4699 19720-19870 sentence denotes However, despite their reduced size, the general morphology and organisation of embryonic structures in Atrxnull conceptuses appeared grossly normal.
T4700 19721-19728 RB denotes However
T4702 19728-19730 , denotes ,
T4703 19730-19737 IN denotes despite
T4704 19738-19743 PRP$ denotes their
T4706 19744-19751 VBN denotes reduced
T4705 19752-19756 NN denotes size
T4707 19756-19758 , denotes ,
T4708 19758-19761 DT denotes the
T4710 19762-19769 JJ denotes general
T4709 19770-19780 NN denotes morphology
T4711 19781-19784 CC denotes and
T4712 19785-19797 NN denotes organisation
T4713 19798-19800 IN denotes of
T4714 19801-19810 JJ denotes embryonic
T4715 19811-19821 NNS denotes structures
T4716 19822-19824 IN denotes in
T4717 19825-19833 JJ denotes Atrxnull
T4718 19834-19845 NNS denotes conceptuses
T4701 19846-19854 VBD denotes appeared
T4719 19855-19862 RB denotes grossly
T4720 19863-19869 JJ denotes normal
T4721 19869-19870 . denotes .
T4722 19870-20050 sentence denotes The amnion and chorion were clearly present and the amniotic, exocoelomic, and ectoplacental cavities were distinguishable, as were all three embryonic germ layers (Figure 5A–5C).
T4723 19871-19874 DT denotes The
T4724 19875-19881 NN denotes amnion
T4726 19882-19885 CC denotes and
T4727 19886-19893 NN denotes chorion
T4725 19894-19898 VBD denotes were
T4728 19899-19906 RB denotes clearly
T4729 19907-19914 JJ denotes present
T4730 19915-19918 CC denotes and
T4731 19919-19922 DT denotes the
T4733 19923-19931 JJ denotes amniotic
T4734 19931-19933 , denotes ,
T4735 19933-19944 JJ denotes exocoelomic
T4736 19944-19946 , denotes ,
T4737 19946-19949 CC denotes and
T4738 19950-19963 JJ denotes ectoplacental
T4732 19964-19972 NNS denotes cavities
T4739 19973-19977 VBD denotes were
T4740 19978-19993 JJ denotes distinguishable
T4741 19993-19995 , denotes ,
T4742 19995-19997 IN denotes as
T4743 19998-20002 VBD denotes were
T4744 20003-20006 DT denotes all
T4746 20007-20012 CD denotes three
T4747 20013-20022 JJ denotes embryonic
T4748 20023-20027 NN denotes germ
T4745 20028-20034 NNS denotes layers
T4749 20035-20036 -LRB- denotes (
T4751 20036-20042 NN denotes Figure
T4750 20043-20045 NN denotes 5A
T4752 20045-20046 SYM denotes –
T4753 20046-20048 NN denotes 5C
T4754 20048-20049 -RRB- denotes )
T4755 20049-20050 . denotes .
T4756 20050-20132 sentence denotes At 8.5 dpc, embryos were dissected free of deciduas, and observed in whole mount.
T4757 20051-20053 IN denotes At
T4759 20054-20057 CD denotes 8.5
T4760 20058-20061 NN denotes dpc
T4761 20061-20063 , denotes ,
T4762 20063-20070 NNS denotes embryos
T4763 20071-20075 VBD denotes were
T4758 20076-20085 VBN denotes dissected
T4764 20086-20090 JJ denotes free
T4765 20091-20093 IN denotes of
T4766 20094-20102 NNS denotes deciduas
T4767 20102-20104 , denotes ,
T4768 20104-20107 CC denotes and
T4769 20108-20116 VBN denotes observed
T4770 20117-20119 IN denotes in
T4771 20120-20125 JJ denotes whole
T4772 20126-20131 NN denotes mount
T4773 20131-20132 . denotes .
T4774 20132-20239 sentence denotes Individual conceptuses were genotyped by PCR using DNA isolated from yolk sac as described in Protocol S1.
T4775 20133-20143 JJ denotes Individual
T4776 20144-20155 NNS denotes conceptuses
T4778 20156-20160 VBD denotes were
T4777 20161-20170 VBN denotes genotyped
T4779 20171-20173 IN denotes by
T4780 20174-20177 NN denotes PCR
T4781 20178-20183 VBG denotes using
T4782 20184-20187 NN denotes DNA
T4783 20188-20196 VBN denotes isolated
T4784 20197-20201 IN denotes from
T4785 20202-20206 NN denotes yolk
T4786 20207-20210 NN denotes sac
T4787 20211-20213 IN denotes as
T4788 20214-20223 VBN denotes described
T4789 20224-20226 IN denotes in
T4790 20227-20235 NN denotes Protocol
T4791 20236-20238 NN denotes S1
T4792 20238-20239 . denotes .
T4793 20239-20389 sentence denotes Consistent with observations at 7.5 dpc, the general morphology of the embryo proper of Atrxnull conceptuses also appeared grossly normal at 8.5 dpc.
T4794 20240-20250 JJ denotes Consistent
T4796 20251-20255 IN denotes with
T4797 20256-20268 NNS denotes observations
T4798 20269-20271 IN denotes at
T4799 20272-20275 CD denotes 7.5
T4800 20276-20279 NN denotes dpc
T4801 20279-20281 , denotes ,
T4802 20281-20284 DT denotes the
T4804 20285-20292 JJ denotes general
T4803 20293-20303 NN denotes morphology
T4805 20304-20306 IN denotes of
T4806 20307-20310 DT denotes the
T4807 20311-20317 NN denotes embryo
T4808 20318-20324 JJ denotes proper
T4809 20325-20327 IN denotes of
T4810 20328-20336 JJ denotes Atrxnull
T4811 20337-20348 NNS denotes conceptuses
T4812 20349-20353 RB denotes also
T4795 20354-20362 VBD denotes appeared
T4813 20363-20370 RB denotes grossly
T4814 20371-20377 JJ denotes normal
T4815 20378-20380 IN denotes at
T4816 20381-20384 CD denotes 8.5
T4817 20385-20388 NN denotes dpc
T4818 20388-20389 . denotes .
T4819 20389-20654 sentence denotes The head fold had clearly formed, and expression of the early mesoderm marker brachyury (T) [16] was detected in the primitive streak and emerging notochord by whole-mount in situ hybridisation (WMISH) (Figure 5F), indicating that Atrxnull embryos had gastrulated.
T4820 20390-20393 DT denotes The
T4822 20394-20398 NN denotes head
T4821 20399-20403 NN denotes fold
T4824 20404-20407 VBD denotes had
T4825 20408-20415 RB denotes clearly
T4823 20416-20422 VBN denotes formed
T4826 20422-20424 , denotes ,
T4827 20424-20427 CC denotes and
T4828 20428-20438 NN denotes expression
T4830 20439-20441 IN denotes of
T4831 20442-20445 DT denotes the
T4833 20446-20451 JJ denotes early
T4834 20452-20460 NN denotes mesoderm
T4835 20461-20467 NN denotes marker
T4832 20468-20477 NN denotes brachyury
T4836 20478-20479 -LRB- denotes (
T4837 20479-20480 NN denotes T
T4838 20480-20481 -RRB- denotes )
T4839 20482-20483 -LRB- denotes [
T4840 20483-20485 CD denotes 16
T4841 20485-20486 -RRB- denotes ]
T4842 20487-20490 VBD denotes was
T4829 20491-20499 VBN denotes detected
T4843 20500-20502 IN denotes in
T4844 20503-20506 DT denotes the
T4846 20507-20516 JJ denotes primitive
T4845 20517-20523 NN denotes streak
T4847 20524-20527 CC denotes and
T4848 20528-20536 VBG denotes emerging
T4849 20537-20546 NN denotes notochord
T4850 20547-20549 IN denotes by
T4851 20550-20555 JJ denotes whole
T4853 20555-20556 HYPH denotes -
T4852 20556-20561 NN denotes mount
T4855 20562-20564 FW denotes in
T4856 20565-20569 FW denotes situ
T4854 20570-20583 NN denotes hybridisation
T4857 20584-20585 -LRB- denotes (
T4858 20585-20590 NN denotes WMISH
T4859 20590-20591 -RRB- denotes )
T4860 20592-20593 -LRB- denotes (
T4862 20593-20599 NN denotes Figure
T4861 20600-20602 NN denotes 5F
T4863 20602-20603 -RRB- denotes )
T4864 20603-20605 , denotes ,
T4865 20605-20615 VBG denotes indicating
T4866 20616-20620 IN denotes that
T4868 20621-20629 JJ denotes Atrxnull
T4869 20630-20637 NNS denotes embryos
T4870 20638-20641 VBD denotes had
T4867 20642-20653 VBN denotes gastrulated
T4871 20653-20654 . denotes .
T4872 20654-21766 sentence denotes Figure 5 Morphology of Atrxnull Embryos at 7.5 dpc and 8.5 dpc Paraffin sections of wild-type or Atrxnull 7.5 dpc embryos (dissected in their deciduas) were stained with haematoxylin (A) or with an anti-ATRX antibody (H-300, Figure 1) (B–E). Photomicrographs C–E show higher magnification images (200×) of the stained sections shown in (B) (40×). Scale bars represent 200 μm (40× magnification) or 40 μm (200× magnification). a, amnion; ac, amniotic cavity; c, chorion; e, epiblast; ec, ectoplacental cavity; ecc, exocoelomic cavity; ep, ectoplacental cone; ne, neural ectoderm; rm, Reichert's membrane; tgc, trophoblast giant cell. (F) Detection of brachyury (T) expression in Atrxnull 8.5 dpc embryo (head fold stage) by WMISH. The genotype was determined by PCR (as shown in Protocol S1) using DNA extracted from yolk sac. hf, head fold; n, emerging notochord; ps, primitive streak. To investigate whether the reduced size of the Atrxnull embryos was due to an increase in apoptosis, we analysed sections of paraffin-embedded 7.5 dpc embryos by TdT-mediated dUTP nick end labeling (TUNEL) assay (Figure 6A).
T4873 21542-21544 TO denotes To
T4874 21545-21556 VB denotes investigate
T4876 21557-21564 IN denotes whether
T4878 21565-21568 DT denotes the
T4880 21569-21576 VBN denotes reduced
T4879 21577-21581 NN denotes size
T4881 21582-21584 IN denotes of
T4882 21585-21588 DT denotes the
T4884 21589-21597 JJ denotes Atrxnull
T4883 21598-21605 NNS denotes embryos
T4877 21606-21609 VBD denotes was
T4885 21610-21613 IN denotes due
T4886 21614-21616 IN denotes to
T4887 21617-21619 DT denotes an
T4888 21620-21628 NN denotes increase
T4889 21629-21631 IN denotes in
T4890 21632-21641 NN denotes apoptosis
T4891 21641-21643 , denotes ,
T4892 21643-21645 PRP denotes we
T4875 21646-21654 VBD denotes analysed
T4893 21655-21663 NNS denotes sections
T4894 21664-21666 IN denotes of
T4895 21667-21675 NN denotes paraffin
T4897 21675-21676 HYPH denotes -
T4896 21676-21684 VBN denotes embedded
T4899 21685-21688 CD denotes 7.5
T4900 21689-21692 NN denotes dpc
T4898 21693-21700 NNS denotes embryos
T4901 21701-21703 IN denotes by
T4902 21704-21707 NN denotes TdT
T4904 21707-21708 HYPH denotes -
T4903 21708-21716 VBN denotes mediated
T4906 21717-21721 NN denotes dUTP
T4907 21722-21726 NN denotes nick
T4908 21727-21730 NN denotes end
T4905 21731-21739 NN denotes labeling
T4910 21740-21741 -LRB- denotes (
T4911 21741-21746 NN denotes TUNEL
T4912 21746-21747 -RRB- denotes )
T4909 21748-21753 NN denotes assay
T4913 21754-21755 -LRB- denotes (
T4915 21755-21761 NN denotes Figure
T4914 21762-21764 NN denotes 6A
T4916 21764-21765 -RRB- denotes )
T4917 21765-21766 . denotes .
T4918 21766-21835 sentence denotes Very few apoptotic cells were detected in wild-type 7.5 dpc embryos.
T4919 21767-21771 RB denotes Very
T4920 21772-21775 JJ denotes few
T4922 21776-21785 JJ denotes apoptotic
T4921 21786-21791 NNS denotes cells
T4924 21792-21796 VBD denotes were
T4923 21797-21805 VBN denotes detected
T4925 21806-21808 IN denotes in
T4926 21809-21813 JJ denotes wild
T4928 21813-21814 HYPH denotes -
T4927 21814-21818 NN denotes type
T4930 21819-21822 CD denotes 7.5
T4931 21823-21826 NN denotes dpc
T4929 21827-21834 NNS denotes embryos
T4932 21834-21835 . denotes .
T4933 21835-21915 sentence denotes In Atrxnull embryos, a slight increase in the apoptotic population was evident.
T4934 21836-21838 IN denotes In
T4936 21839-21847 JJ denotes Atrxnull
T4937 21848-21855 NNS denotes embryos
T4938 21855-21857 , denotes ,
T4939 21857-21858 DT denotes a
T4941 21859-21865 JJ denotes slight
T4940 21866-21874 NN denotes increase
T4942 21875-21877 IN denotes in
T4943 21878-21881 DT denotes the
T4945 21882-21891 JJ denotes apoptotic
T4944 21892-21902 NN denotes population
T4935 21903-21906 VBD denotes was
T4946 21907-21914 JJ denotes evident
T4947 21914-21915 . denotes .
T4948 21915-22170 sentence denotes However, consistent with our observation of a grossly normal apoptotic index in Atrxnull ES cells (Figure S2), the apoptotic response observed in Atrxnull embryos was also not uniform, but was restricted to a low number of scattered TUNEL-positive cells.
T4949 21916-21923 RB denotes However
T4951 21923-21925 , denotes ,
T4952 21925-21935 JJ denotes consistent
T4953 21936-21940 IN denotes with
T4954 21941-21944 PRP$ denotes our
T4955 21945-21956 NN denotes observation
T4956 21957-21959 IN denotes of
T4957 21960-21961 DT denotes a
T4959 21962-21969 RB denotes grossly
T4960 21970-21976 JJ denotes normal
T4961 21977-21986 JJ denotes apoptotic
T4958 21987-21992 NN denotes index
T4962 21993-21995 IN denotes in
T4963 21996-22004 JJ denotes Atrxnull
T4964 22005-22007 NN denotes ES
T4965 22008-22013 NNS denotes cells
T4966 22014-22015 -LRB- denotes (
T4968 22015-22021 NN denotes Figure
T4967 22022-22024 NN denotes S2
T4969 22024-22025 -RRB- denotes )
T4970 22025-22027 , denotes ,
T4971 22027-22030 DT denotes the
T4973 22031-22040 JJ denotes apoptotic
T4972 22041-22049 NN denotes response
T4974 22050-22058 VBN denotes observed
T4975 22059-22061 IN denotes in
T4976 22062-22070 JJ denotes Atrxnull
T4977 22071-22078 NNS denotes embryos
T4950 22079-22082 VBD denotes was
T4978 22083-22087 RB denotes also
T4979 22088-22091 RB denotes not
T4980 22092-22099 JJ denotes uniform
T4981 22099-22101 , denotes ,
T4982 22101-22104 CC denotes but
T4983 22105-22108 VBD denotes was
T4984 22109-22119 VBN denotes restricted
T4985 22120-22122 IN denotes to
T4986 22123-22124 DT denotes a
T4988 22125-22128 JJ denotes low
T4987 22129-22135 NN denotes number
T4989 22136-22138 IN denotes of
T4990 22139-22148 VBN denotes scattered
T4992 22149-22154 NN denotes TUNEL
T4994 22154-22155 HYPH denotes -
T4993 22155-22163 JJ denotes positive
T4991 22164-22169 NNS denotes cells
T4995 22169-22170 . denotes .
T4996 22170-22471 sentence denotes Since this small apoptotic response is unlikely to account fully for the dramatic size deficit observed in Atrxnull embryos, a possible proliferation defect was also investigated by immunohistochemical staining of 7.5 dpc embryo sections for the mitosis marker phosphorylated (Ser10) histone H3 [13].
T4997 22171-22176 IN denotes Since
T4999 22177-22181 DT denotes this
T5001 22182-22187 JJ denotes small
T5002 22188-22197 JJ denotes apoptotic
T5000 22198-22206 NN denotes response
T4998 22207-22209 VBZ denotes is
T5004 22210-22218 JJ denotes unlikely
T5005 22219-22221 TO denotes to
T5006 22222-22229 VB denotes account
T5007 22230-22235 RB denotes fully
T5008 22236-22239 IN denotes for
T5009 22240-22243 DT denotes the
T5011 22244-22252 JJ denotes dramatic
T5012 22253-22257 NN denotes size
T5010 22258-22265 NN denotes deficit
T5013 22266-22274 VBN denotes observed
T5014 22275-22277 IN denotes in
T5015 22278-22286 JJ denotes Atrxnull
T5016 22287-22294 NNS denotes embryos
T5017 22294-22296 , denotes ,
T5018 22296-22297 DT denotes a
T5020 22298-22306 JJ denotes possible
T5021 22307-22320 NN denotes proliferation
T5019 22321-22327 NN denotes defect
T5022 22328-22331 VBD denotes was
T5023 22332-22336 RB denotes also
T5003 22337-22349 VBN denotes investigated
T5024 22350-22352 IN denotes by
T5025 22353-22372 JJ denotes immunohistochemical
T5026 22373-22381 NN denotes staining
T5027 22382-22384 IN denotes of
T5028 22385-22388 CD denotes 7.5
T5029 22389-22392 NN denotes dpc
T5030 22393-22399 NN denotes embryo
T5031 22400-22408 NNS denotes sections
T5032 22409-22412 IN denotes for
T5033 22413-22416 DT denotes the
T5035 22417-22424 NN denotes mitosis
T5036 22425-22431 NN denotes marker
T5037 22432-22446 VBN denotes phosphorylated
T5038 22447-22448 -LRB- denotes (
T5039 22448-22453 NN denotes Ser10
T5040 22453-22454 -RRB- denotes )
T5041 22455-22462 NN denotes histone
T5034 22463-22465 NN denotes H3
T5042 22466-22467 -LRB- denotes [
T5043 22467-22469 CD denotes 13
T5044 22469-22470 -RRB- denotes ]
T5045 22470-22471 . denotes .
T5046 22471-22652 sentence denotes Relative to the very high mitotic index observed in wild-type embryos, the proportion of mitotic cells observed in Atrxnull embryos at 7.5 dpc was dramatically reduced (Figure 6B).
T5047 22472-22480 JJ denotes Relative
T5049 22481-22483 IN denotes to
T5050 22484-22487 DT denotes the
T5052 22488-22492 RB denotes very
T5053 22493-22497 JJ denotes high
T5054 22498-22505 JJ denotes mitotic
T5051 22506-22511 NN denotes index
T5055 22512-22520 VBN denotes observed
T5056 22521-22523 IN denotes in
T5057 22524-22528 JJ denotes wild
T5059 22528-22529 HYPH denotes -
T5058 22529-22533 NN denotes type
T5060 22534-22541 NNS denotes embryos
T5061 22541-22543 , denotes ,
T5062 22543-22546 DT denotes the
T5063 22547-22557 NN denotes proportion
T5064 22558-22560 IN denotes of
T5065 22561-22568 JJ denotes mitotic
T5066 22569-22574 NNS denotes cells
T5067 22575-22583 VBN denotes observed
T5068 22584-22586 IN denotes in
T5069 22587-22595 JJ denotes Atrxnull
T5070 22596-22603 NNS denotes embryos
T5071 22604-22606 IN denotes at
T5072 22607-22610 CD denotes 7.5
T5073 22611-22614 NN denotes dpc
T5074 22615-22618 VBD denotes was
T5075 22619-22631 RB denotes dramatically
T5048 22632-22639 VBN denotes reduced
T5076 22640-22641 -LRB- denotes (
T5078 22641-22647 NN denotes Figure
T5077 22648-22650 NN denotes 6B
T5079 22650-22651 -RRB- denotes )
T5080 22651-22652 . denotes .
T5081 22652-22866 sentence denotes Taken together, these results suggest that the size deficit observed in Atrxnull embryos prior to lethality largely reflects a proliferative defect, with a minor but indirect contribution from increased apoptosis.
T5082 22653-22658 VBN denotes Taken
T5084 22659-22667 RB denotes together
T5085 22667-22669 , denotes ,
T5086 22669-22674 DT denotes these
T5087 22675-22682 NNS denotes results
T5083 22683-22690 VBP denotes suggest
T5088 22691-22695 IN denotes that
T5090 22696-22699 DT denotes the
T5092 22700-22704 NN denotes size
T5091 22705-22712 NN denotes deficit
T5093 22713-22721 VBN denotes observed
T5094 22722-22724 IN denotes in
T5095 22725-22733 JJ denotes Atrxnull
T5096 22734-22741 NNS denotes embryos
T5097 22742-22747 JJ denotes prior
T5098 22748-22750 IN denotes to
T5099 22751-22760 NN denotes lethality
T5100 22761-22768 RB denotes largely
T5089 22769-22777 VBZ denotes reflects
T5101 22778-22779 DT denotes a
T5103 22780-22793 JJ denotes proliferative
T5102 22794-22800 NN denotes defect
T5104 22800-22802 , denotes ,
T5105 22802-22806 IN denotes with
T5106 22807-22808 DT denotes a
T5108 22809-22814 JJ denotes minor
T5109 22815-22818 CC denotes but
T5110 22819-22827 JJ denotes indirect
T5107 22828-22840 NN denotes contribution
T5111 22841-22845 IN denotes from
T5112 22846-22855 VBN denotes increased
T5113 22856-22865 NN denotes apoptosis
T5114 22865-22866 . denotes .
T5115 22866-23092 sentence denotes Although a growth defect was also observed in Atrxnull ES cells (Figure 3A), in contrast to the Atrxnull embryos, the mitotic index of the ES cell population (as measured with the same antibody) was not depleted (Figure S1B).
T5116 22867-22875 IN denotes Although
T5118 22876-22877 DT denotes a
T5120 22878-22884 NN denotes growth
T5119 22885-22891 NN denotes defect
T5121 22892-22895 VBD denotes was
T5122 22896-22900 RB denotes also
T5117 22901-22909 VBN denotes observed
T5124 22910-22912 IN denotes in
T5125 22913-22921 JJ denotes Atrxnull
T5127 22922-22924 NN denotes ES
T5126 22925-22930 NNS denotes cells
T5128 22931-22932 -LRB- denotes (
T5130 22932-22938 NN denotes Figure
T5129 22939-22941 NN denotes 3A
T5131 22941-22942 -RRB- denotes )
T5132 22942-22944 , denotes ,
T5133 22944-22946 IN denotes in
T5134 22947-22955 NN denotes contrast
T5135 22956-22958 IN denotes to
T5136 22959-22962 DT denotes the
T5138 22963-22971 JJ denotes Atrxnull
T5137 22972-22979 NNS denotes embryos
T5139 22979-22981 , denotes ,
T5140 22981-22984 DT denotes the
T5142 22985-22992 JJ denotes mitotic
T5141 22993-22998 NN denotes index
T5143 22999-23001 IN denotes of
T5144 23002-23005 DT denotes the
T5146 23006-23008 NN denotes ES
T5147 23009-23013 NN denotes cell
T5145 23014-23024 NN denotes population
T5148 23025-23026 -LRB- denotes (
T5149 23026-23028 IN denotes as
T5150 23029-23037 VBN denotes measured
T5151 23038-23042 IN denotes with
T5152 23043-23046 DT denotes the
T5154 23047-23051 JJ denotes same
T5153 23052-23060 NN denotes antibody
T5155 23060-23061 -RRB- denotes )
T5156 23062-23065 VBD denotes was
T5157 23066-23069 RB denotes not
T5123 23070-23078 VBN denotes depleted
T5158 23079-23080 -LRB- denotes (
T5160 23080-23086 NN denotes Figure
T5159 23087-23090 NN denotes S1B
T5161 23090-23091 -RRB- denotes )
T5162 23091-23092 . denotes .
T5163 23092-23354 sentence denotes These observations suggest that the mitotic defect observed in embryos is unlikely to be a direct, cell-autonomous effect of the absence of Atrx, and is more likely to be a secondary effect resulting from the failure to develop a normal trophoblast (see below).
T5164 23093-23098 DT denotes These
T5165 23099-23111 NNS denotes observations
T5166 23112-23119 VBP denotes suggest
T5167 23120-23124 IN denotes that
T5169 23125-23128 DT denotes the
T5171 23129-23136 JJ denotes mitotic
T5170 23137-23143 NN denotes defect
T5172 23144-23152 VBN denotes observed
T5173 23153-23155 IN denotes in
T5174 23156-23163 NNS denotes embryos
T5168 23164-23166 VBZ denotes is
T5175 23167-23175 JJ denotes unlikely
T5176 23176-23178 TO denotes to
T5177 23179-23181 VB denotes be
T5178 23182-23183 DT denotes a
T5180 23184-23190 JJ denotes direct
T5181 23190-23192 , denotes ,
T5182 23192-23196 NN denotes cell
T5184 23196-23197 HYPH denotes -
T5183 23197-23207 JJ denotes autonomous
T5179 23208-23214 NN denotes effect
T5185 23215-23217 IN denotes of
T5186 23218-23221 DT denotes the
T5187 23222-23229 NN denotes absence
T5188 23230-23232 IN denotes of
T5189 23233-23237 NN denotes Atrx
T5190 23237-23239 , denotes ,
T5191 23239-23242 CC denotes and
T5192 23243-23245 VBZ denotes is
T5193 23246-23250 RBR denotes more
T5194 23251-23257 JJ denotes likely
T5195 23258-23260 TO denotes to
T5196 23261-23263 VB denotes be
T5197 23264-23265 DT denotes a
T5199 23266-23275 JJ denotes secondary
T5198 23276-23282 NN denotes effect
T5200 23283-23292 VBG denotes resulting
T5201 23293-23297 IN denotes from
T5202 23298-23301 DT denotes the
T5203 23302-23309 NN denotes failure
T5204 23310-23312 TO denotes to
T5205 23313-23320 VB denotes develop
T5206 23321-23322 DT denotes a
T5208 23323-23329 JJ denotes normal
T5207 23330-23341 NN denotes trophoblast
T5209 23342-23343 -LRB- denotes (
T5210 23343-23346 VB denotes see
T5211 23347-23352 RB denotes below
T5212 23352-23353 -RRB- denotes )
T5213 23353-23354 . denotes .
T5495 24022-24035 NN denotes Trophectoderm
T5496 24036-24043 NN denotes Failure
T5497 24044-24046 IN denotes in
T5498 24047-24055 JJ denotes Atrxnull
T5499 24056-24063 NNS denotes Embryos
T5500 24063-24288 sentence denotes Whole-mount observation of 8.5 dpc embryos revealed that, in contrast to the basically normal although delayed morphology of the embryo itself, the extraembryonic tissues of Atrxnull conceptuses appeared highly disorganised.
T5501 24064-24069 JJ denotes Whole
T5503 24069-24070 HYPH denotes -
T5502 24070-24075 NN denotes mount
T5504 24076-24087 NN denotes observation
T5506 24088-24090 IN denotes of
T5507 24091-24094 CD denotes 8.5
T5508 24095-24098 NN denotes dpc
T5509 24099-24106 NNS denotes embryos
T5505 24107-24115 VBD denotes revealed
T5510 24116-24120 IN denotes that
T5512 24120-24122 , denotes ,
T5513 24122-24124 IN denotes in
T5514 24125-24133 NN denotes contrast
T5515 24134-24136 IN denotes to
T5516 24137-24140 DT denotes the
T5518 24141-24150 RB denotes basically
T5519 24151-24157 JJ denotes normal
T5520 24158-24166 IN denotes although
T5521 24167-24174 VBN denotes delayed
T5517 24175-24185 NN denotes morphology
T5522 24186-24188 IN denotes of
T5523 24189-24192 DT denotes the
T5524 24193-24199 NN denotes embryo
T5525 24200-24206 PRP denotes itself
T5526 24206-24208 , denotes ,
T5527 24208-24211 DT denotes the
T5529 24212-24226 JJ denotes extraembryonic
T5528 24227-24234 NNS denotes tissues
T5530 24235-24237 IN denotes of
T5531 24238-24246 JJ denotes Atrxnull
T5532 24247-24258 NNS denotes conceptuses
T5511 24259-24267 VBD denotes appeared
T5533 24268-24274 RB denotes highly
T5534 24275-24287 JJ denotes disorganised
T5535 24287-24288 . denotes .
T5536 24288-24540 sentence denotes When embryos were removed from deciduas, the surrounding trophectoderm layer appeared dramatically reduced in Atrxnull embryos relative to wild-type littermates, and the underlying ectoplacental cone appeared reduced and abnormally shaped (Figure 7A).
T5537 24289-24293 WRB denotes When
T5539 24294-24301 NNS denotes embryos
T5540 24302-24306 VBD denotes were
T5538 24307-24314 VBN denotes removed
T5542 24315-24319 IN denotes from
T5543 24320-24328 NNS denotes deciduas
T5544 24328-24330 , denotes ,
T5545 24330-24333 DT denotes the
T5547 24334-24345 VBG denotes surrounding
T5548 24346-24359 NN denotes trophectoderm
T5546 24360-24365 NN denotes layer
T5541 24366-24374 VBD denotes appeared
T5549 24375-24387 RB denotes dramatically
T5550 24388-24395 JJ denotes reduced
T5551 24396-24398 IN denotes in
T5552 24399-24407 JJ denotes Atrxnull
T5553 24408-24415 NNS denotes embryos
T5554 24416-24424 JJ denotes relative
T5555 24425-24427 IN denotes to
T5556 24428-24432 JJ denotes wild
T5558 24432-24433 HYPH denotes -
T5557 24433-24437 NN denotes type
T5559 24438-24449 NNS denotes littermates
T5560 24449-24451 , denotes ,
T5561 24451-24454 CC denotes and
T5562 24455-24458 DT denotes the
T5564 24459-24469 VBG denotes underlying
T5565 24470-24483 JJ denotes ectoplacental
T5563 24484-24488 NN denotes cone
T5566 24489-24497 VBD denotes appeared
T5567 24498-24505 JJ denotes reduced
T5568 24506-24509 CC denotes and
T5569 24510-24520 RB denotes abnormally
T5570 24521-24527 VBN denotes shaped
T5571 24528-24529 -LRB- denotes (
T5573 24529-24535 NN denotes Figure
T5572 24536-24538 NN denotes 7A
T5574 24538-24539 -RRB- denotes )
T5575 24539-24540 . denotes .
T5576 24540-24735 sentence denotes Vacated deciduas surrounding 8.5 dpc wild-type and Atrxnull embryos were bisected and analysed by WMISH for expression of placental lactogen-1 (Pl-1), a marker of terminally differentiated TGCs.
T5577 24541-24548 VBN denotes Vacated
T5578 24549-24557 NNS denotes deciduas
T5580 24558-24569 VBG denotes surrounding
T5581 24570-24573 CD denotes 8.5
T5582 24574-24577 NN denotes dpc
T5584 24578-24582 JJ denotes wild
T5586 24582-24583 HYPH denotes -
T5585 24583-24587 NN denotes type
T5587 24588-24591 CC denotes and
T5588 24592-24600 JJ denotes Atrxnull
T5583 24601-24608 NNS denotes embryos
T5589 24609-24613 VBD denotes were
T5579 24614-24622 VBN denotes bisected
T5590 24623-24626 CC denotes and
T5591 24627-24635 VBN denotes analysed
T5592 24636-24638 IN denotes by
T5593 24639-24644 NN denotes WMISH
T5594 24645-24648 IN denotes for
T5595 24649-24659 NN denotes expression
T5596 24660-24662 IN denotes of
T5597 24663-24672 JJ denotes placental
T5598 24673-24681 NN denotes lactogen
T5599 24681-24682 HYPH denotes -
T5600 24682-24683 CD denotes 1
T5601 24684-24685 -LRB- denotes (
T5602 24685-24687 NN denotes Pl
T5603 24687-24688 HYPH denotes -
T5604 24688-24689 CD denotes 1
T5605 24689-24690 -RRB- denotes )
T5606 24690-24692 , denotes ,
T5607 24692-24693 DT denotes a
T5608 24694-24700 NN denotes marker
T5609 24701-24703 IN denotes of
T5610 24704-24714 RB denotes terminally
T5611 24715-24729 VBN denotes differentiated
T5612 24730-24734 NNS denotes TGCs
T5613 24734-24735 . denotes .
T5614 24735-24923 sentence denotes The number of Pl-1-expressing cells attached to the decidual wall after removal of the embryo is an indication of the density of trophoblast cells surrounding each implantation site [17].
T5615 24736-24739 DT denotes The
T5616 24740-24746 NN denotes number
T5618 24747-24749 IN denotes of
T5619 24750-24752 NN denotes Pl
T5621 24752-24753 HYPH denotes -
T5622 24753-24754 CD denotes 1
T5623 24754-24755 HYPH denotes -
T5620 24755-24765 VBG denotes expressing
T5624 24766-24771 NNS denotes cells
T5625 24772-24780 VBN denotes attached
T5626 24781-24783 IN denotes to
T5627 24784-24787 DT denotes the
T5629 24788-24796 JJ denotes decidual
T5628 24797-24801 NN denotes wall
T5630 24802-24807 IN denotes after
T5631 24808-24815 NN denotes removal
T5632 24816-24818 IN denotes of
T5633 24819-24822 DT denotes the
T5634 24823-24829 NN denotes embryo
T5617 24830-24832 VBZ denotes is
T5635 24833-24835 DT denotes an
T5636 24836-24846 NN denotes indication
T5637 24847-24849 IN denotes of
T5638 24850-24853 DT denotes the
T5639 24854-24861 NN denotes density
T5640 24862-24864 IN denotes of
T5641 24865-24876 NN denotes trophoblast
T5642 24877-24882 NNS denotes cells
T5643 24883-24894 VBG denotes surrounding
T5644 24895-24899 DT denotes each
T5646 24900-24912 NN denotes implantation
T5645 24913-24917 NN denotes site
T5647 24918-24919 -LRB- denotes [
T5648 24919-24921 CD denotes 17
T5649 24921-24922 -RRB- denotes ]
T5650 24922-24923 . denotes .
T5651 24923-25282 sentence denotes We found that the population of Pl-1-expressing cells was depleted in the decidual implantation sites containing Atrxnull embryos relative to those containing wild-type littermates (Figure 7B); this was also apparent at 7.5 dpc, as determined by immunohistochemical staining of paraffin sections of embryos in deciduas with an anti-Pl-1 antibody (Figure 7C).
T5652 24924-24926 PRP denotes We
T5653 24927-24932 VBD denotes found
T5655 24933-24937 IN denotes that
T5657 24938-24941 DT denotes the
T5658 24942-24952 NN denotes population
T5659 24953-24955 IN denotes of
T5660 24956-24958 NN denotes Pl
T5662 24958-24959 HYPH denotes -
T5663 24959-24960 CD denotes 1
T5664 24960-24961 HYPH denotes -
T5661 24961-24971 VBG denotes expressing
T5665 24972-24977 NNS denotes cells
T5666 24978-24981 VBD denotes was
T5656 24982-24990 VBN denotes depleted
T5667 24991-24993 IN denotes in
T5668 24994-24997 DT denotes the
T5670 24998-25006 JJ denotes decidual
T5671 25007-25019 NN denotes implantation
T5669 25020-25025 NNS denotes sites
T5672 25026-25036 VBG denotes containing
T5673 25037-25045 JJ denotes Atrxnull
T5674 25046-25053 NNS denotes embryos
T5675 25054-25062 JJ denotes relative
T5676 25063-25065 IN denotes to
T5677 25066-25071 DT denotes those
T5678 25072-25082 VBG denotes containing
T5679 25083-25087 JJ denotes wild
T5681 25087-25088 HYPH denotes -
T5680 25088-25092 NN denotes type
T5682 25093-25104 NNS denotes littermates
T5683 25105-25106 -LRB- denotes (
T5685 25106-25112 NN denotes Figure
T5684 25113-25115 NN denotes 7B
T5686 25115-25116 -RRB- denotes )
T5687 25116-25117 : denotes ;
T5688 25118-25122 DT denotes this
T5654 25123-25126 VBD denotes was
T5689 25127-25131 RB denotes also
T5690 25132-25140 JJ denotes apparent
T5691 25141-25143 IN denotes at
T5692 25144-25147 CD denotes 7.5
T5693 25148-25151 NN denotes dpc
T5694 25151-25153 , denotes ,
T5695 25153-25155 IN denotes as
T5696 25156-25166 VBN denotes determined
T5697 25167-25169 IN denotes by
T5698 25170-25189 JJ denotes immunohistochemical
T5699 25190-25198 NN denotes staining
T5700 25199-25201 IN denotes of
T5701 25202-25210 NN denotes paraffin
T5702 25211-25219 NNS denotes sections
T5703 25220-25222 IN denotes of
T5704 25223-25230 NNS denotes embryos
T5705 25231-25233 IN denotes in
T5706 25234-25242 NNS denotes deciduas
T5707 25243-25247 IN denotes with
T5708 25248-25250 DT denotes an
T5710 25251-25258 JJ denotes anti-Pl
T5712 25258-25259 HYPH denotes -
T5711 25259-25260 CD denotes 1
T5709 25261-25269 NN denotes antibody
T5713 25270-25271 -LRB- denotes (
T5715 25271-25277 NN denotes Figure
T5714 25278-25280 NN denotes 7C
T5716 25280-25281 -RRB- denotes )
T5717 25281-25282 . denotes .
T5718 25282-25453 sentence denotes A TGC deficiency in the absence of Atrx is consistent with the observation that Atrx is highly expressed in giant cells surrounding wild-type 7.5 dpc embryos (Figure 5E).
T5719 25283-25284 DT denotes A
T5721 25285-25288 NN denotes TGC
T5720 25289-25299 NN denotes deficiency
T5723 25300-25302 IN denotes in
T5724 25303-25306 DT denotes the
T5725 25307-25314 NN denotes absence
T5726 25315-25317 IN denotes of
T5727 25318-25322 NN denotes Atrx
T5722 25323-25325 VBZ denotes is
T5728 25326-25336 JJ denotes consistent
T5729 25337-25341 IN denotes with
T5730 25342-25345 DT denotes the
T5731 25346-25357 NN denotes observation
T5732 25358-25362 IN denotes that
T5734 25363-25367 NN denotes Atrx
T5733 25368-25370 VBZ denotes is
T5735 25371-25377 RB denotes highly
T5736 25378-25387 JJ denotes expressed
T5737 25388-25390 IN denotes in
T5738 25391-25396 JJ denotes giant
T5739 25397-25402 NNS denotes cells
T5740 25403-25414 VBG denotes surrounding
T5741 25415-25419 JJ denotes wild
T5743 25419-25420 HYPH denotes -
T5742 25420-25424 NN denotes type
T5745 25425-25428 CD denotes 7.5
T5746 25429-25432 NN denotes dpc
T5744 25433-25440 NNS denotes embryos
T5747 25441-25442 -LRB- denotes (
T5749 25442-25448 NN denotes Figure
T5748 25449-25451 NN denotes 5E
T5750 25451-25452 -RRB- denotes )
T5751 25452-25453 . denotes .
T5752 25453-27595 sentence denotes Figure 7 Trophectoderm Defect in Atrxnull Embryos (A) 8.5 dpc embryos were dissected from surrounding decidual tissue and observed in whole mount. The genotype of each (indicated above) was determined by PCR using DNA extracted from whole embryos after photography. In the left image, the wild-type female (three-somite stage, left) is surrounded by trophoblast (t) while the trophoblast component surrounding the Atrxnull males (at headfold/presomite [middle] and two-somite stages [right], respectively) is severely depleted. In the right image, the trophoblast has been dissected away from the embryonic region of the wild-type embryo, to reveal the small, abnormally shaped ectoplacental cone (epc) of the mutant littermates. (B) WMISH to detect expression of Pl-1 (a marker of TGCs) at the implantation sites in vacated deciduas that had contained 8.5 dpc wild-type (Atrx WT/WT) or Atrxnull (Atrx Δ18Δneo/Y) embryos. The genotype was determined by PCR using DNA extracted from whole embryos. TGCs are stained with Pl-1. (C) Paraffin sections of wild-type or Atrxnull 7.5 dpc embryos (dissected in their deciduas) were stained with an anti-Pl-1 antibody. The presence or absence of Atrx in each embryo was determined by staining adjacent sections with the anti-ATRX antibody (H-300) as in Figure 5 (unpublished data). (D) Examples of 5-d blastocyst outgrowth cultures. Extensive trophoblast outgrowing from the inner cell mass (icm) was observed in all genotypes. The Atrx genotype and sex of the blastocyst indicated were determined by PCR. To investigate whether the trophoblast defect was restricted to the production of secondary TGCs (produced by diploid precursors in the ectoplacental cone and derived originally from the polar trophectoderm overlying the inner cell mass of the blastocyst) or also affected the production of primary TGCs (resulting from differentiation of the mural trophectoderm of the blastocyst), blastocysts (3.5 dpc) from crosses between Atrx WT/flox females and GATA1-Cre homozygous transgenic males (Atrx WT/Y;GATA1-Cre +/+) were cultured in vitro for 5 d to monitor outgrowth of the primary trophoblast.
T5753 27001-27003 TO denotes To
T5754 27004-27015 VB denotes investigate
T5756 27016-27023 IN denotes whether
T5758 27024-27027 DT denotes the
T5760 27028-27039 NN denotes trophoblast
T5759 27040-27046 NN denotes defect
T5761 27047-27050 VBD denotes was
T5757 27051-27061 VBN denotes restricted
T5762 27062-27064 IN denotes to
T5763 27065-27068 DT denotes the
T5764 27069-27079 NN denotes production
T5765 27080-27082 IN denotes of
T5766 27083-27092 JJ denotes secondary
T5767 27093-27097 NNS denotes TGCs
T5768 27098-27099 -LRB- denotes (
T5769 27099-27107 VBN denotes produced
T5770 27108-27110 IN denotes by
T5771 27111-27118 NN denotes diploid
T5772 27119-27129 NNS denotes precursors
T5773 27130-27132 IN denotes in
T5774 27133-27136 DT denotes the
T5776 27137-27150 JJ denotes ectoplacental
T5775 27151-27155 NN denotes cone
T5777 27156-27159 CC denotes and
T5778 27160-27167 VBN denotes derived
T5779 27168-27178 RB denotes originally
T5780 27179-27183 IN denotes from
T5781 27184-27187 DT denotes the
T5783 27188-27193 JJ denotes polar
T5782 27194-27207 NN denotes trophectoderm
T5784 27208-27217 VBG denotes overlying
T5785 27218-27221 DT denotes the
T5787 27222-27227 JJ denotes inner
T5788 27228-27232 NN denotes cell
T5786 27233-27237 NN denotes mass
T5789 27238-27240 IN denotes of
T5790 27241-27244 DT denotes the
T5791 27245-27255 NN denotes blastocyst
T5792 27255-27256 -RRB- denotes )
T5793 27257-27259 CC denotes or
T5794 27260-27264 RB denotes also
T5795 27265-27273 VBD denotes affected
T5796 27274-27277 DT denotes the
T5797 27278-27288 NN denotes production
T5798 27289-27291 IN denotes of
T5799 27292-27299 JJ denotes primary
T5800 27300-27304 NNS denotes TGCs
T5801 27305-27306 -LRB- denotes (
T5802 27306-27315 VBG denotes resulting
T5803 27316-27320 IN denotes from
T5804 27321-27336 NN denotes differentiation
T5805 27337-27339 IN denotes of
T5806 27340-27343 DT denotes the
T5808 27344-27349 JJ denotes mural
T5807 27350-27363 NN denotes trophectoderm
T5809 27364-27366 IN denotes of
T5810 27367-27370 DT denotes the
T5811 27371-27381 NN denotes blastocyst
T5812 27381-27382 -RRB- denotes )
T5813 27382-27384 , denotes ,
T5814 27384-27395 NNS denotes blastocysts
T5815 27396-27397 -LRB- denotes (
T5817 27397-27400 CD denotes 3.5
T5816 27401-27404 NN denotes dpc
T5818 27404-27405 -RRB- denotes )
T5819 27406-27410 IN denotes from
T5820 27411-27418 NNS denotes crosses
T5821 27419-27426 IN denotes between
T5822 27427-27434 NN denotes Atrx WT
T5824 27434-27435 HYPH denotes /
T5823 27435-27439 NN denotes flox
T5825 27440-27447 NNS denotes females
T5826 27448-27451 CC denotes and
T5827 27452-27457 NN denotes GATA1
T5829 27457-27458 HYPH denotes -
T5828 27458-27461 NN denotes Cre
T5830 27462-27472 JJ denotes homozygous
T5832 27473-27483 JJ denotes transgenic
T5831 27484-27489 NNS denotes males
T5833 27490-27491 -LRB- denotes (
T5834 27491-27498 NN denotes Atrx WT
T5836 27498-27499 HYPH denotes /
T5835 27499-27500 NN denotes Y
T5837 27500-27501 : denotes ;
T5838 27501-27506 NN denotes GATA1
T5840 27506-27507 HYPH denotes -
T5839 27507-27510 NN denotes Cre
T5841 27511-27512 SYM denotes +
T5842 27512-27513 HYPH denotes /
T5843 27513-27514 SYM denotes +
T5844 27514-27515 -RRB- denotes )
T5845 27516-27520 VBD denotes were
T5755 27521-27529 VBN denotes cultured
T5846 27530-27532 FW denotes in
T5847 27533-27538 FW denotes vitro
T5848 27539-27542 IN denotes for
T5849 27543-27544 CD denotes 5
T5850 27545-27546 NNS denotes d
T5851 27547-27549 TO denotes to
T5852 27550-27557 VB denotes monitor
T5853 27558-27567 NN denotes outgrowth
T5854 27568-27570 IN denotes of
T5855 27571-27574 DT denotes the
T5857 27575-27582 JJ denotes primary
T5856 27583-27594 NN denotes trophoblast
T5858 27594-27595 . denotes .
T5859 27595-27770 sentence denotes After 5 d, individual blastocyst cultures were scored for the extent of primary trophoblast outgrowth, and the Atrx genotype and sex of the blastocyst were determined by PCR.
T5860 27596-27601 IN denotes After
T5862 27602-27603 CD denotes 5
T5863 27604-27605 NNS denotes d
T5864 27605-27607 , denotes ,
T5865 27607-27617 JJ denotes individual
T5867 27618-27628 NN denotes blastocyst
T5866 27629-27637 NNS denotes cultures
T5868 27638-27642 VBD denotes were
T5861 27643-27649 VBN denotes scored
T5869 27650-27653 IN denotes for
T5870 27654-27657 DT denotes the
T5871 27658-27664 NN denotes extent
T5872 27665-27667 IN denotes of
T5873 27668-27675 JJ denotes primary
T5875 27676-27687 NN denotes trophoblast
T5874 27688-27697 NN denotes outgrowth
T5876 27697-27699 , denotes ,
T5877 27699-27702 CC denotes and
T5878 27703-27706 DT denotes the
T5880 27707-27711 NN denotes Atrx
T5879 27712-27720 NN denotes genotype
T5882 27721-27724 CC denotes and
T5883 27725-27728 NN denotes sex
T5884 27729-27731 IN denotes of
T5885 27732-27735 DT denotes the
T5886 27736-27746 NN denotes blastocyst
T5887 27747-27751 VBD denotes were
T5881 27752-27762 VBN denotes determined
T5888 27763-27765 IN denotes by
T5889 27766-27769 NN denotes PCR
T5890 27769-27770 . denotes .
T5891 27770-27943 sentence denotes Most blastocysts hatched from the zona pellucida within 24 h, and trophoblast cells spreading out from the inner cell mass could usually be detected within 48 h of culture.
T5892 27771-27775 JJS denotes Most
T5893 27776-27787 NNS denotes blastocysts
T5894 27788-27795 VBN denotes hatched
T5895 27796-27800 IN denotes from
T5896 27801-27804 DT denotes the
T5898 27805-27809 NN denotes zona
T5897 27810-27819 NN denotes pellucida
T5899 27820-27826 IN denotes within
T5900 27827-27829 CD denotes 24
T5901 27830-27831 NNS denotes h
T5902 27831-27833 , denotes ,
T5903 27833-27836 CC denotes and
T5904 27837-27848 NN denotes trophoblast
T5905 27849-27854 NNS denotes cells
T5907 27855-27864 VBG denotes spreading
T5908 27865-27868 RP denotes out
T5909 27869-27873 IN denotes from
T5910 27874-27877 DT denotes the
T5912 27878-27883 JJ denotes inner
T5913 27884-27888 NN denotes cell
T5911 27889-27893 NN denotes mass
T5914 27894-27899 MD denotes could
T5915 27900-27907 RB denotes usually
T5916 27908-27910 VB denotes be
T5906 27911-27919 VBN denotes detected
T5917 27920-27926 IN denotes within
T5918 27927-27929 CD denotes 48
T5919 27930-27931 NNS denotes h
T5920 27932-27934 IN denotes of
T5921 27935-27942 NN denotes culture
T5922 27942-27943 . denotes .
T5923 27943-28306 sentence denotes No difference was observed in the rate or extent of trophoblast outgrowth over 5 d of culture between Atrxnull blastocysts (Atrx null/Y, n = 6) and blastocysts bearing an Atrx WT allele (Atrx WT/WT, n = 6; Atrx WT/null, n = 6; Atrx WT/Y, n = 6) (examples shown in Figure 7D), suggesting that the defect specifically involves the secondary giant cell compartment.
T5924 27944-27946 DT denotes No
T5925 27947-27957 NN denotes difference
T5927 27958-27961 VBD denotes was
T5926 27962-27970 VBN denotes observed
T5928 27971-27973 IN denotes in
T5929 27974-27977 DT denotes the
T5930 27978-27982 NN denotes rate
T5931 27983-27985 CC denotes or
T5932 27986-27992 NN denotes extent
T5933 27993-27995 IN denotes of
T5934 27996-28007 NN denotes trophoblast
T5935 28008-28017 NN denotes outgrowth
T5936 28018-28022 IN denotes over
T5937 28023-28024 CD denotes 5
T5938 28025-28026 NNS denotes d
T5939 28027-28029 IN denotes of
T5940 28030-28037 NN denotes culture
T5941 28038-28045 IN denotes between
T5942 28046-28054 JJ denotes Atrxnull
T5943 28055-28066 NNS denotes blastocysts
T5944 28067-28068 -LRB- denotes (
T5946 28068-28077 JJ denotes Atrx null
T5947 28077-28078 HYPH denotes /
T5945 28078-28079 NN denotes Y
T5948 28079-28081 , denotes ,
T5949 28081-28082 NN denotes n
T5951 28083-28084 SYM denotes =
T5950 28085-28086 CD denotes 6
T5952 28086-28087 -RRB- denotes )
T5953 28088-28091 CC denotes and
T5954 28092-28103 NNS denotes blastocysts
T5955 28104-28111 VBG denotes bearing
T5956 28112-28114 DT denotes an
T5958 28115-28122 NN denotes Atrx WT
T5957 28123-28129 NN denotes allele
T5959 28130-28131 -LRB- denotes (
T5961 28131-28138 NN denotes Atrx WT
T5963 28138-28139 HYPH denotes /
T5962 28139-28141 NN denotes WT
T5965 28141-28143 , denotes ,
T5966 28143-28144 NN denotes n
T5967 28145-28146 SYM denotes =
T5964 28147-28148 CD denotes 6
T5968 28148-28149 : denotes ;
T5969 28150-28157 NN denotes Atrx WT
T5971 28157-28158 HYPH denotes /
T5970 28158-28162 NN denotes null
T5973 28162-28164 , denotes ,
T5974 28164-28165 NN denotes n
T5975 28166-28167 SYM denotes =
T5972 28168-28169 CD denotes 6
T5976 28169-28170 : denotes ;
T5977 28171-28178 NN denotes Atrx WT
T5979 28178-28179 HYPH denotes /
T5978 28179-28180 NN denotes Y
T5980 28180-28182 , denotes ,
T5981 28182-28183 NN denotes n
T5982 28184-28185 SYM denotes =
T5960 28186-28187 CD denotes 6
T5983 28187-28188 -RRB- denotes )
T5984 28189-28190 -LRB- denotes (
T5986 28190-28198 NNS denotes examples
T5985 28199-28204 VBN denotes shown
T5987 28205-28207 IN denotes in
T5988 28208-28214 NN denotes Figure
T5989 28215-28217 NN denotes 7D
T5990 28217-28218 -RRB- denotes )
T5991 28218-28220 , denotes ,
T5992 28220-28230 VBG denotes suggesting
T5993 28231-28235 IN denotes that
T5995 28236-28239 DT denotes the
T5996 28240-28246 NN denotes defect
T5997 28247-28259 RB denotes specifically
T5994 28260-28268 VBZ denotes involves
T5998 28269-28272 DT denotes the
T6000 28273-28282 JJ denotes secondary
T6001 28283-28288 JJ denotes giant
T6002 28289-28293 NN denotes cell
T5999 28294-28305 NN denotes compartment
T6003 28305-28306 . denotes .
T6004 28306-28422 sentence denotes This is consistent with the observation that Atrxnull conceptuses implant successfully and survive to gastrulation.
T6005 28307-28311 DT denotes This
T6006 28312-28314 VBZ denotes is
T6007 28315-28325 JJ denotes consistent
T6008 28326-28330 IN denotes with
T6009 28331-28334 DT denotes the
T6010 28335-28346 NN denotes observation
T6011 28347-28351 IN denotes that
T6013 28352-28360 JJ denotes Atrxnull
T6014 28361-28372 NNS denotes conceptuses
T6012 28373-28380 VBP denotes implant
T6015 28381-28393 RB denotes successfully
T6016 28394-28397 CC denotes and
T6017 28398-28405 VBP denotes survive
T6018 28406-28408 IN denotes to
T6019 28409-28421 NN denotes gastrulation
T6020 28421-28422 . denotes .
T6021 28422-28568 sentence denotes Taken together, these data suggest that loss of Atrx results in a defect in formation of the secondary trophoblast that is apparent from 7.5 dpc.
T6022 28423-28428 VBN denotes Taken
T6024 28429-28437 RB denotes together
T6025 28437-28439 , denotes ,
T6026 28439-28444 DT denotes these
T6027 28445-28449 NNS denotes data
T6023 28450-28457 VBP denotes suggest
T6028 28458-28462 IN denotes that
T6030 28463-28467 NN denotes loss
T6031 28468-28470 IN denotes of
T6032 28471-28475 NN denotes Atrx
T6029 28476-28483 VBZ denotes results
T6033 28484-28486 IN denotes in
T6034 28487-28488 DT denotes a
T6035 28489-28495 NN denotes defect
T6036 28496-28498 IN denotes in
T6037 28499-28508 NN denotes formation
T6038 28509-28511 IN denotes of
T6039 28512-28515 DT denotes the
T6041 28516-28525 JJ denotes secondary
T6040 28526-28537 NN denotes trophoblast
T6042 28538-28542 WDT denotes that
T6043 28543-28545 VBZ denotes is
T6044 28546-28554 JJ denotes apparent
T6045 28555-28559 IN denotes from
T6046 28560-28563 CD denotes 7.5
T6047 28564-28567 NN denotes dpc
T6048 28567-28568 . denotes .
T6049 28568-28813 sentence denotes Despite initiating normal organisation in the embryo proper, Atrxnull conceptuses exhibit a proliferative defect by 7.5 dpc and die by around 9.5 dpc, probably due to a nutritional deficit resulting from failure to develop a normal trophoblast.
T6050 28569-28576 IN denotes Despite
T6052 28577-28587 VBG denotes initiating
T6053 28588-28594 JJ denotes normal
T6054 28595-28607 NN denotes organisation
T6055 28608-28610 IN denotes in
T6056 28611-28614 DT denotes the
T6057 28615-28621 NN denotes embryo
T6058 28622-28628 JJ denotes proper
T6059 28628-28630 , denotes ,
T6060 28630-28638 JJ denotes Atrxnull
T6061 28639-28650 NNS denotes conceptuses
T6051 28651-28658 VBP denotes exhibit
T6062 28659-28660 DT denotes a
T6064 28661-28674 JJ denotes proliferative
T6063 28675-28681 NN denotes defect
T6065 28682-28684 IN denotes by
T6066 28685-28688 CD denotes 7.5
T6067 28689-28692 NN denotes dpc
T6068 28693-28696 CC denotes and
T6069 28697-28700 VBP denotes die
T6070 28701-28703 IN denotes by
T6071 28704-28710 IN denotes around
T6072 28711-28714 CD denotes 9.5
T6073 28715-28718 NN denotes dpc
T6074 28718-28720 , denotes ,
T6075 28720-28728 RB denotes probably
T6076 28729-28732 IN denotes due
T6077 28733-28735 IN denotes to
T6078 28736-28737 DT denotes a
T6080 28738-28749 JJ denotes nutritional
T6079 28750-28757 NN denotes deficit
T6081 28758-28767 VBG denotes resulting
T6082 28768-28772 IN denotes from
T6083 28773-28780 NN denotes failure
T6084 28781-28783 TO denotes to
T6085 28784-28791 VB denotes develop
T6086 28792-28793 DT denotes a
T6088 28794-28800 JJ denotes normal
T6087 28801-28812 NN denotes trophoblast
T6089 28812-28813 . denotes .
T6480 28815-28821 NN denotes Escape
T6481 28822-28826 IN denotes from
T6482 28827-28836 VBN denotes Imprinted
T6483 28837-28849 NN denotes Inactivation
T6484 28850-28852 IN denotes of
T6485 28853-28856 DT denotes the
T6487 28857-28867 RB denotes Paternally
T6488 28868-28877 VBN denotes Inherited
T6489 28878-28885 NN denotes Atrx WT
T6486 28886-28892 NN denotes Allele
T6490 28893-28895 IN denotes in
T6491 28896-28910 JJ denotes Extraembryonic
T6492 28911-28918 NNS denotes Tissues
T6493 28919-28921 IN denotes of
T6494 28922-28929 NN denotes Carrier
T6496 28930-28936 JJ denotes Female
T6495 28937-28941 NNS denotes Mice
T6497 28941-29287 sentence denotes Female mice carrying an Atrx null allele (Atrx WT/null;GATA1-cre +/−) were detected at 9.5 dpc (Table 1) and recovered at birth (unpublished data), although at both time points the number of carrier females was lower than that of wild-type (Atrx WT/WT;GATA1-cre +/−) females, suggesting that a proportion of carrier female embryos died in utero.
T6498 28942-28948 JJ denotes Female
T6499 28949-28953 NNS denotes mice
T6501 28954-28962 VBG denotes carrying
T6502 28963-28965 DT denotes an
T6504 28966-28975 JJ denotes Atrx null
T6503 28976-28982 NN denotes allele
T6505 28983-28984 -LRB- denotes (
T6507 28984-28991 NN denotes Atrx WT
T6509 28991-28992 HYPH denotes /
T6508 28992-28996 NN denotes null
T6510 28996-28997 : denotes ;
T6511 28997-29002 NN denotes GATA1
T6512 29002-29003 HYPH denotes -
T6506 29003-29006 NN denotes cre
T6513 29007-29008 SYM denotes +
T6514 29008-29009 HYPH denotes /
T6515 29009-29010 SYM denotes −
T6516 29010-29011 -RRB- denotes )
T6517 29012-29016 VBD denotes were
T6500 29017-29025 VBN denotes detected
T6518 29026-29028 IN denotes at
T6519 29029-29032 CD denotes 9.5
T6520 29033-29036 NN denotes dpc
T6521 29037-29038 -LRB- denotes (
T6522 29038-29043 NN denotes Table
T6523 29044-29045 CD denotes 1
T6524 29045-29046 -RRB- denotes )
T6525 29047-29050 CC denotes and
T6526 29051-29060 VBN denotes recovered
T6527 29061-29063 IN denotes at
T6528 29064-29069 NN denotes birth
T6529 29070-29071 -LRB- denotes (
T6531 29071-29082 JJ denotes unpublished
T6530 29083-29087 NNS denotes data
T6532 29087-29088 -RRB- denotes )
T6533 29088-29090 , denotes ,
T6534 29090-29098 IN denotes although
T6536 29099-29101 IN denotes at
T6537 29102-29106 CC denotes both
T6539 29107-29111 NN denotes time
T6538 29112-29118 NNS denotes points
T6540 29119-29122 DT denotes the
T6541 29123-29129 NN denotes number
T6542 29130-29132 IN denotes of
T6543 29133-29140 NN denotes carrier
T6544 29141-29148 NNS denotes females
T6535 29149-29152 VBD denotes was
T6545 29153-29158 JJR denotes lower
T6546 29159-29163 IN denotes than
T6547 29164-29168 DT denotes that
T6548 29169-29171 IN denotes of
T6549 29172-29176 JJ denotes wild
T6551 29176-29177 HYPH denotes -
T6550 29177-29181 NN denotes type
T6553 29182-29183 -LRB- denotes (
T6555 29183-29190 NN denotes Atrx WT
T6557 29190-29191 HYPH denotes /
T6556 29191-29193 NN denotes WT
T6558 29193-29194 : denotes ;
T6559 29194-29199 NN denotes GATA1
T6560 29199-29200 HYPH denotes -
T6554 29200-29203 NN denotes cre
T6561 29204-29205 SYM denotes +
T6562 29205-29206 HYPH denotes /
T6563 29206-29207 SYM denotes −
T6564 29207-29208 -RRB- denotes )
T6552 29209-29216 NNS denotes females
T6565 29216-29218 , denotes ,
T6566 29218-29228 VBG denotes suggesting
T6567 29229-29233 IN denotes that
T6569 29234-29235 DT denotes a
T6570 29236-29246 NN denotes proportion
T6571 29247-29249 IN denotes of
T6572 29250-29257 NN denotes carrier
T6574 29258-29264 JJ denotes female
T6573 29265-29272 NNS denotes embryos
T6568 29273-29277 VBD denotes died
T6575 29278-29280 FW denotes in
T6576 29281-29286 FW denotes utero
T6577 29286-29287 . denotes .
T6578 29287-29431 sentence denotes Surviving adult carrier female mice were not phenotypically normal and exhibited mild behavioural abnormalities, although some could reproduce.
T6579 29288-29297 VBG denotes Surviving
T6581 29298-29303 JJ denotes adult
T6582 29304-29311 NN denotes carrier
T6583 29312-29318 JJ denotes female
T6580 29319-29323 NNS denotes mice
T6584 29324-29328 VBD denotes were
T6585 29329-29332 RB denotes not
T6586 29333-29347 RB denotes phenotypically
T6587 29348-29354 JJ denotes normal
T6588 29355-29358 CC denotes and
T6589 29359-29368 VBD denotes exhibited
T6590 29369-29373 JJ denotes mild
T6592 29374-29385 JJ denotes behavioural
T6591 29386-29399 NNS denotes abnormalities
T6593 29399-29401 , denotes ,
T6594 29401-29409 IN denotes although
T6596 29410-29414 DT denotes some
T6597 29415-29420 MD denotes could
T6595 29421-29430 VB denotes reproduce
T6598 29430-29431 . denotes .
T6599 29431-29591 sentence denotes For all Atrx WT/null carrier female embryos presented in Table 1, the Atrx WT allele was paternally derived, while the Atrx null allele was maternally derived.
T6600 29432-29435 IN denotes For
T6602 29436-29439 DT denotes all
T6604 29440-29447 NN denotes Atrx WT
T6606 29447-29448 HYPH denotes /
T6605 29448-29452 JJ denotes null
T6607 29453-29460 NN denotes carrier
T6608 29461-29467 JJ denotes female
T6603 29468-29475 NNS denotes embryos
T6609 29476-29485 VBN denotes presented
T6610 29486-29488 IN denotes in
T6611 29489-29494 NN denotes Table
T6612 29495-29496 CD denotes 1
T6613 29496-29498 , denotes ,
T6614 29498-29501 DT denotes the
T6616 29502-29509 NN denotes Atrx WT
T6615 29510-29516 NN denotes allele
T6617 29517-29520 VBD denotes was
T6618 29521-29531 RB denotes paternally
T6601 29532-29539 VBN denotes derived
T6619 29539-29541 , denotes ,
T6620 29541-29546 IN denotes while
T6622 29547-29550 DT denotes the
T6624 29551-29560 JJ denotes Atrx null
T6623 29561-29567 NN denotes allele
T6625 29568-29571 VBD denotes was
T6626 29572-29582 RB denotes maternally
T6621 29583-29590 VBN denotes derived
T6627 29590-29591 . denotes .
T6628 29591-29830 sentence denotes In the mouse, X chromosome inactivation is subject to parental imprinting in the trophectoderm and primitive endoderm lineages that give rise to the extraembryonic tissues, resulting in inactivation of the paternal X chromosome (Xp) [18].
T6629 29592-29594 IN denotes In
T6631 29595-29598 DT denotes the
T6632 29599-29604 NN denotes mouse
T6633 29604-29606 , denotes ,
T6634 29606-29607 NN denotes X
T6635 29608-29618 NN denotes chromosome
T6636 29619-29631 NN denotes inactivation
T6630 29632-29634 VBZ denotes is
T6637 29635-29642 JJ denotes subject
T6638 29643-29645 IN denotes to
T6639 29646-29654 JJ denotes parental
T6640 29655-29665 NN denotes imprinting
T6641 29666-29668 IN denotes in
T6642 29669-29672 DT denotes the
T6643 29673-29686 NN denotes trophectoderm
T6644 29687-29690 CC denotes and
T6645 29691-29700 JJ denotes primitive
T6647 29701-29709 NN denotes endoderm
T6646 29710-29718 NNS denotes lineages
T6648 29719-29723 WDT denotes that
T6649 29724-29728 VBP denotes give
T6650 29729-29733 NN denotes rise
T6651 29734-29736 IN denotes to
T6652 29737-29740 DT denotes the
T6654 29741-29755 JJ denotes extraembryonic
T6653 29756-29763 NNS denotes tissues
T6655 29763-29765 , denotes ,
T6656 29765-29774 VBG denotes resulting
T6657 29775-29777 IN denotes in
T6658 29778-29790 NN denotes inactivation
T6659 29791-29793 IN denotes of
T6660 29794-29797 DT denotes the
T6662 29798-29806 JJ denotes paternal
T6663 29807-29808 NN denotes X
T6661 29809-29819 NN denotes chromosome
T6664 29820-29821 -LRB- denotes (
T6665 29821-29823 NN denotes Xp
T6666 29823-29824 -RRB- denotes )
T6667 29825-29826 -LRB- denotes [
T6668 29826-29828 CD denotes 18
T6669 29828-29829 -RRB- denotes ]
T6670 29829-29830 . denotes .
T6671 29830-29959 sentence denotes In contrast, in tissues of the embryo proper (derived from the inner cell mass of the blastocyst) X-inactivation is random [19].
T6672 29831-29833 IN denotes In
T6674 29834-29842 NN denotes contrast
T6675 29842-29844 , denotes ,
T6676 29844-29846 IN denotes in
T6677 29847-29854 NNS denotes tissues
T6678 29855-29857 IN denotes of
T6679 29858-29861 DT denotes the
T6680 29862-29868 NN denotes embryo
T6681 29869-29875 JJ denotes proper
T6682 29876-29877 -LRB- denotes (
T6683 29877-29884 VBN denotes derived
T6684 29885-29889 IN denotes from
T6685 29890-29893 DT denotes the
T6687 29894-29899 JJ denotes inner
T6688 29900-29904 NN denotes cell
T6686 29905-29909 NN denotes mass
T6689 29910-29912 IN denotes of
T6690 29913-29916 DT denotes the
T6691 29917-29927 NN denotes blastocyst
T6692 29927-29928 -RRB- denotes )
T6693 29929-29930 NN denotes X
T6695 29930-29931 HYPH denotes -
T6694 29931-29943 NN denotes inactivation
T6673 29944-29946 VBZ denotes is
T6696 29947-29953 JJ denotes random
T6697 29954-29955 -LRB- denotes [
T6698 29955-29957 CD denotes 19
T6699 29957-29958 -RRB- denotes ]
T6700 29958-29959 . denotes .
T6701 29959-30292 sentence denotes Thus, in the extraembryonic compartment of Atrx WT/null carrier females, normal imprinted X-inactivation would be expected to result in silencing of the paternally derived Atrx WT allele, leaving only the Atrx null allele on the active maternal X (Xm) and thereby render the extraembryonic tissues null for full-length Atrx protein.
T6702 29960-29964 RB denotes Thus
T6704 29964-29966 , denotes ,
T6705 29966-29968 IN denotes in
T6706 29969-29972 DT denotes the
T6708 29973-29987 JJ denotes extraembryonic
T6707 29988-29999 NN denotes compartment
T6709 30000-30002 IN denotes of
T6710 30003-30010 NN denotes Atrx WT
T6712 30010-30011 HYPH denotes /
T6711 30011-30015 JJ denotes null
T6714 30016-30023 NN denotes carrier
T6713 30024-30031 NNS denotes females
T6715 30031-30033 , denotes ,
T6716 30033-30039 JJ denotes normal
T6718 30040-30049 VBN denotes imprinted
T6719 30050-30051 NN denotes X
T6720 30051-30052 HYPH denotes -
T6717 30052-30064 NN denotes inactivation
T6721 30065-30070 MD denotes would
T6722 30071-30073 VB denotes be
T6703 30074-30082 VBN denotes expected
T6723 30083-30085 TO denotes to
T6724 30086-30092 VB denotes result
T6725 30093-30095 IN denotes in
T6726 30096-30105 VBG denotes silencing
T6727 30106-30108 IN denotes of
T6728 30109-30112 DT denotes the
T6730 30113-30123 RB denotes paternally
T6731 30124-30131 VBN denotes derived
T6732 30132-30139 NN denotes Atrx WT
T6729 30140-30146 NN denotes allele
T6733 30146-30148 , denotes ,
T6734 30148-30155 VBG denotes leaving
T6735 30156-30160 RB denotes only
T6737 30161-30164 DT denotes the
T6738 30165-30174 JJ denotes Atrx null
T6736 30175-30181 NN denotes allele
T6739 30182-30184 IN denotes on
T6740 30185-30188 DT denotes the
T6742 30189-30195 JJ denotes active
T6743 30196-30204 JJ denotes maternal
T6741 30205-30206 NN denotes X
T6744 30207-30208 -LRB- denotes (
T6745 30208-30210 NN denotes Xm
T6746 30210-30211 -RRB- denotes )
T6747 30212-30215 CC denotes and
T6748 30216-30223 RB denotes thereby
T6749 30224-30230 VB denotes render
T6750 30231-30234 DT denotes the
T6752 30235-30249 JJ denotes extraembryonic
T6751 30250-30257 NNS denotes tissues
T6753 30258-30262 JJ denotes null
T6754 30263-30266 IN denotes for
T6755 30267-30271 JJ denotes full
T6757 30271-30272 HYPH denotes -
T6756 30272-30278 NN denotes length
T6759 30279-30283 NN denotes Atrx
T6758 30284-30291 NN denotes protein
T6760 30291-30292 . denotes .
T6761 30292-30387 sentence denotes However, the absence of Atrx in the extraembryonic compartment is lethal in Atrx null/Y males.
T6762 30293-30300 RB denotes However
T6764 30300-30302 , denotes ,
T6765 30302-30305 DT denotes the
T6766 30306-30313 NN denotes absence
T6767 30314-30316 IN denotes of
T6768 30317-30321 NN denotes Atrx
T6769 30322-30324 IN denotes in
T6770 30325-30328 DT denotes the
T6772 30329-30343 JJ denotes extraembryonic
T6771 30344-30355 NN denotes compartment
T6763 30356-30358 VBZ denotes is
T6773 30359-30365 JJ denotes lethal
T6774 30366-30368 IN denotes in
T6775 30369-30378 JJ denotes Atrx null
T6777 30378-30379 HYPH denotes /
T6776 30379-30380 NN denotes Y
T6778 30381-30386 NNS denotes males
T6779 30386-30387 . denotes .
T6780 30387-30577 sentence denotes This suggested the possibility of an escape from imprinted inactivation of the paternally derived Atrx WT allele in the extraembryonic compartment of a proportion of carrier female embryos.
T6781 30388-30392 DT denotes This
T6782 30393-30402 VBD denotes suggested
T6783 30403-30406 DT denotes the
T6784 30407-30418 NN denotes possibility
T6785 30419-30421 IN denotes of
T6786 30422-30424 DT denotes an
T6787 30425-30431 NN denotes escape
T6788 30432-30436 IN denotes from
T6789 30437-30446 VBN denotes imprinted
T6790 30447-30459 NN denotes inactivation
T6791 30460-30462 IN denotes of
T6792 30463-30466 DT denotes the
T6794 30467-30477 RB denotes paternally
T6795 30478-30485 VBN denotes derived
T6796 30486-30493 NN denotes Atrx WT
T6793 30494-30500 NN denotes allele
T6797 30501-30503 IN denotes in
T6798 30504-30507 DT denotes the
T6800 30508-30522 JJ denotes extraembryonic
T6799 30523-30534 NN denotes compartment
T6801 30535-30537 IN denotes of
T6802 30538-30539 DT denotes a
T6803 30540-30550 NN denotes proportion
T6804 30551-30553 IN denotes of
T6805 30554-30561 NN denotes carrier
T6807 30562-30568 JJ denotes female
T6806 30569-30576 NNS denotes embryos
T6808 30576-30577 . denotes .
T6809 30577-30845 sentence denotes To investigate further, we crossed homozygous Atrx flox/flox females and homozygous GATA1-cre transgenic males (Atrx WT/Y;GATA1-cre +/+), which would be expected to yield only Atrxnull males (Atrxnull/Y;GATA1-cre +/−) and carrier females (Atrx WT/null;GATA1-cre +/−).
T6810 30578-30580 TO denotes To
T6811 30581-30592 VB denotes investigate
T6813 30593-30600 RB denotes further
T6814 30600-30602 , denotes ,
T6815 30602-30604 PRP denotes we
T6812 30605-30612 VBD denotes crossed
T6816 30613-30623 JJ denotes homozygous
T6818 30624-30633 NN denotes Atrx flox
T6820 30633-30634 HYPH denotes /
T6819 30634-30638 NN denotes flox
T6817 30639-30646 NNS denotes females
T6821 30647-30650 CC denotes and
T6822 30651-30661 JJ denotes homozygous
T6824 30662-30667 NN denotes GATA1
T6826 30667-30668 HYPH denotes -
T6825 30668-30671 NN denotes cre
T6827 30672-30682 JJ denotes transgenic
T6823 30683-30688 NNS denotes males
T6828 30689-30690 -LRB- denotes (
T6830 30690-30697 NN denotes Atrx WT
T6832 30697-30698 HYPH denotes /
T6831 30698-30699 NN denotes Y
T6833 30699-30700 : denotes ;
T6834 30700-30705 NN denotes GATA1
T6835 30705-30706 HYPH denotes -
T6829 30706-30709 NN denotes cre
T6836 30710-30711 SYM denotes +
T6837 30711-30712 HYPH denotes /
T6838 30712-30713 SYM denotes +
T6839 30713-30714 -RRB- denotes )
T6840 30714-30716 , denotes ,
T6841 30716-30721 WDT denotes which
T6843 30722-30727 MD denotes would
T6844 30728-30730 VB denotes be
T6842 30731-30739 VBN denotes expected
T6845 30740-30742 TO denotes to
T6846 30743-30748 VB denotes yield
T6847 30749-30753 RB denotes only
T6849 30754-30762 JJ denotes Atrxnull
T6848 30763-30768 NNS denotes males
T6850 30769-30770 -LRB- denotes (
T6852 30770-30778 JJ denotes Atrxnull
T6854 30778-30779 HYPH denotes /
T6853 30779-30780 NN denotes Y
T6855 30780-30781 : denotes ;
T6856 30781-30786 NN denotes GATA1
T6857 30786-30787 HYPH denotes -
T6851 30787-30790 NN denotes cre
T6858 30791-30792 SYM denotes +
T6859 30792-30793 HYPH denotes /
T6860 30793-30794 SYM denotes −
T6861 30794-30795 -RRB- denotes )
T6862 30796-30799 CC denotes and
T6863 30800-30807 NN denotes carrier
T6864 30808-30815 NNS denotes females
T6865 30816-30817 -LRB- denotes (
T6867 30817-30824 NN denotes Atrx WT
T6869 30824-30825 HYPH denotes /
T6868 30825-30829 NN denotes null
T6870 30829-30830 : denotes ;
T6871 30830-30835 NN denotes GATA1
T6872 30835-30836 HYPH denotes -
T6866 30836-30839 NN denotes cre
T6873 30840-30841 SYM denotes +
T6874 30841-30842 HYPH denotes /
T6875 30842-30843 SYM denotes −
T6876 30843-30844 -RRB- denotes )
T6877 30844-30845 . denotes .
T6878 30845-30915 sentence denotes In these carrier females, the Atrx WT allele is paternally inherited.
T6879 30846-30848 IN denotes In
T6881 30849-30854 DT denotes these
T6883 30855-30862 NN denotes carrier
T6882 30863-30870 NNS denotes females
T6884 30870-30872 , denotes ,
T6885 30872-30875 DT denotes the
T6887 30876-30883 NN denotes Atrx WT
T6886 30884-30890 NN denotes allele
T6888 30891-30893 VBZ denotes is
T6889 30894-30904 RB denotes paternally
T6880 30905-30914 VBN denotes inherited
T6890 30914-30915 . denotes .
T6891 30915-31107 sentence denotes Embryos were dissected in their deciduas at 7.5 dpc, and paraffin sections were stained with anti-ATRX antibody, along with sections from a wild-type 7.5 dpc embryo for comparison (Figure 8).
T6892 30916-30923 NNS denotes Embryos
T6894 30924-30928 VBD denotes were
T6893 30929-30938 VBN denotes dissected
T6895 30939-30941 IN denotes in
T6896 30942-30947 PRP$ denotes their
T6897 30948-30956 NNS denotes deciduas
T6898 30957-30959 IN denotes at
T6899 30960-30963 CD denotes 7.5
T6900 30964-30967 NN denotes dpc
T6901 30967-30969 , denotes ,
T6902 30969-30972 CC denotes and
T6903 30973-30981 NN denotes paraffin
T6904 30982-30990 NNS denotes sections
T6906 30991-30995 VBD denotes were
T6905 30996-31003 VBN denotes stained
T6907 31004-31008 IN denotes with
T6908 31009-31018 JJ denotes anti-ATRX
T6909 31019-31027 NN denotes antibody
T6910 31027-31029 , denotes ,
T6911 31029-31034 IN denotes along
T6913 31035-31039 IN denotes with
T6912 31040-31048 NNS denotes sections
T6914 31049-31053 IN denotes from
T6915 31054-31055 DT denotes a
T6917 31056-31060 JJ denotes wild
T6919 31060-31061 HYPH denotes -
T6918 31061-31065 NN denotes type
T6920 31066-31069 CD denotes 7.5
T6921 31070-31073 NN denotes dpc
T6916 31074-31080 NN denotes embryo
T6922 31081-31084 IN denotes for
T6923 31085-31095 NN denotes comparison
T6924 31096-31097 -LRB- denotes (
T6925 31097-31103 NN denotes Figure
T6926 31104-31105 CD denotes 8
T6927 31105-31106 -RRB- denotes )
T6928 31106-31107 . denotes .
T6929 31107-31251 sentence denotes As described above, Atrx expression was detected in every cell in the epiblast (embryo proper) region of wild-type 7.5 dpc embryos (Figure 8B).
T6930 31108-31110 IN denotes As
T6931 31111-31120 VBN denotes described
T6933 31121-31126 RB denotes above
T6934 31126-31128 , denotes ,
T6935 31128-31132 NN denotes Atrx
T6936 31133-31143 NN denotes expression
T6937 31144-31147 VBD denotes was
T6932 31148-31156 VBN denotes detected
T6938 31157-31159 IN denotes in
T6939 31160-31165 DT denotes every
T6940 31166-31170 NN denotes cell
T6941 31171-31173 IN denotes in
T6942 31174-31177 DT denotes the
T6944 31178-31186 NN denotes epiblast
T6945 31187-31188 -LRB- denotes (
T6946 31188-31194 NN denotes embryo
T6947 31195-31201 JJ denotes proper
T6948 31201-31202 -RRB- denotes )
T6943 31203-31209 NN denotes region
T6949 31210-31212 IN denotes of
T6950 31213-31217 JJ denotes wild
T6952 31217-31218 HYPH denotes -
T6951 31218-31222 NN denotes type
T6954 31223-31226 CD denotes 7.5
T6955 31227-31230 NN denotes dpc
T6953 31231-31238 NNS denotes embryos
T6956 31239-31240 -LRB- denotes (
T6958 31240-31246 NN denotes Figure
T6957 31247-31249 NN denotes 8B
T6959 31249-31250 -RRB- denotes )
T6960 31250-31251 . denotes .
T6961 31251-31608 sentence denotes In contrast, the epiblast region of carrier female embryos was composed of a mosaic of small clusters of Atrx-positive cells (in which the Atrx null allele on Xm had been inactivated) and Atrx-negative cells (in which the Atrx WT allele on Xp had been inactivated), indicating that the Atrx gene was subject to normal random X-inactivation in the epiblast.
T6962 31252-31254 IN denotes In
T6964 31255-31263 NN denotes contrast
T6965 31263-31265 , denotes ,
T6966 31265-31268 DT denotes the
T6968 31269-31277 NN denotes epiblast
T6967 31278-31284 NN denotes region
T6969 31285-31287 IN denotes of
T6970 31288-31295 NN denotes carrier
T6972 31296-31302 JJ denotes female
T6971 31303-31310 NNS denotes embryos
T6973 31311-31314 VBD denotes was
T6963 31315-31323 VBN denotes composed
T6974 31324-31326 IN denotes of
T6975 31327-31328 DT denotes a
T6976 31329-31335 NN denotes mosaic
T6977 31336-31338 IN denotes of
T6978 31339-31344 JJ denotes small
T6979 31345-31353 NNS denotes clusters
T6980 31354-31356 IN denotes of
T6981 31357-31361 NN denotes Atrx
T6983 31361-31362 HYPH denotes -
T6982 31362-31370 JJ denotes positive
T6984 31371-31376 NNS denotes cells
T6985 31377-31378 -LRB- denotes (
T6986 31378-31380 IN denotes in
T6988 31381-31386 WDT denotes which
T6989 31387-31390 DT denotes the
T6991 31391-31400 JJ denotes Atrx null
T6990 31401-31407 NN denotes allele
T6992 31408-31410 IN denotes on
T6993 31411-31413 NN denotes Xm
T6994 31414-31417 VBD denotes had
T6995 31418-31422 VBN denotes been
T6987 31423-31434 VBN denotes inactivated
T6996 31434-31435 -RRB- denotes )
T6997 31436-31439 CC denotes and
T6998 31440-31444 NN denotes Atrx
T7000 31444-31445 HYPH denotes -
T6999 31445-31453 JJ denotes negative
T7001 31454-31459 NNS denotes cells
T7002 31460-31461 -LRB- denotes (
T7003 31461-31463 IN denotes in
T7005 31464-31469 WDT denotes which
T7006 31470-31473 DT denotes the
T7008 31474-31481 NN denotes Atrx WT
T7007 31482-31488 NN denotes allele
T7009 31489-31491 IN denotes on
T7010 31492-31494 NN denotes Xp
T7011 31495-31498 VBD denotes had
T7012 31499-31503 VBN denotes been
T7004 31504-31515 VBN denotes inactivated
T7013 31515-31516 -RRB- denotes )
T7014 31516-31518 , denotes ,
T7015 31518-31528 VBG denotes indicating
T7016 31529-31533 IN denotes that
T7018 31534-31537 DT denotes the
T7020 31538-31542 NN denotes Atrx
T7019 31543-31547 NN denotes gene
T7017 31548-31551 VBD denotes was
T7021 31552-31559 JJ denotes subject
T7022 31560-31562 IN denotes to
T7023 31563-31569 JJ denotes normal
T7025 31570-31576 JJ denotes random
T7026 31577-31578 NN denotes X
T7027 31578-31579 HYPH denotes -
T7024 31579-31591 NN denotes inactivation
T7028 31592-31594 IN denotes in
T7029 31595-31598 DT denotes the
T7030 31599-31607 NN denotes epiblast
T7031 31607-31608 . denotes .
T7032 31608-31786 sentence denotes Remarkably, clear Atrx expression could also be detected in the extraembryonic tissues of carrier females, as shown in the extraembryonic-derived chorionic ectoderm (Figure 8C).
T7033 31609-31619 RB denotes Remarkably
T7035 31619-31621 , denotes ,
T7036 31621-31626 JJ denotes clear
T7038 31627-31631 NN denotes Atrx
T7037 31632-31642 NN denotes expression
T7039 31643-31648 MD denotes could
T7040 31649-31653 RB denotes also
T7041 31654-31656 VB denotes be
T7034 31657-31665 VBN denotes detected
T7042 31666-31668 IN denotes in
T7043 31669-31672 DT denotes the
T7045 31673-31687 JJ denotes extraembryonic
T7044 31688-31695 NNS denotes tissues
T7046 31696-31698 IN denotes of
T7047 31699-31706 NN denotes carrier
T7048 31707-31714 NNS denotes females
T7049 31714-31716 , denotes ,
T7050 31716-31718 IN denotes as
T7051 31719-31724 VBN denotes shown
T7052 31725-31727 IN denotes in
T7053 31728-31731 DT denotes the
T7055 31732-31746 JJ denotes extraembryonic
T7057 31746-31747 HYPH denotes -
T7056 31747-31754 VBN denotes derived
T7058 31755-31764 JJ denotes chorionic
T7054 31765-31773 NN denotes ectoderm
T7059 31774-31775 -LRB- denotes (
T7061 31775-31781 NN denotes Figure
T7060 31782-31784 NN denotes 8C
T7062 31784-31785 -RRB- denotes )
T7063 31785-31786 . denotes .
T7064 31786-31867 sentence denotes Atrx expression could be detected in almost all cells of the chorionic ectoderm.
T7065 31787-31791 NN denotes Atrx
T7066 31792-31802 NN denotes expression
T7068 31803-31808 MD denotes could
T7069 31809-31811 VB denotes be
T7067 31812-31820 VBN denotes detected
T7070 31821-31823 IN denotes in
T7071 31824-31830 RB denotes almost
T7072 31831-31834 DT denotes all
T7073 31835-31840 NNS denotes cells
T7074 31841-31843 IN denotes of
T7075 31844-31847 DT denotes the
T7077 31848-31857 JJ denotes chorionic
T7076 31858-31866 NN denotes ectoderm
T7078 31866-31867 . denotes .
T7079 31867-31980 sentence denotes Atrx expression was also clearly detected in other extraembryonic structures, including TGCs (unpublished data).
T7080 31868-31872 NN denotes Atrx
T7081 31873-31883 NN denotes expression
T7083 31884-31887 VBD denotes was
T7084 31888-31892 RB denotes also
T7085 31893-31900 RB denotes clearly
T7082 31901-31909 VBN denotes detected
T7086 31910-31912 IN denotes in
T7087 31913-31918 JJ denotes other
T7089 31919-31933 JJ denotes extraembryonic
T7088 31934-31944 NNS denotes structures
T7090 31944-31946 , denotes ,
T7091 31946-31955 VBG denotes including
T7092 31956-31960 NNS denotes TGCs
T7093 31961-31962 -LRB- denotes (
T7095 31962-31973 JJ denotes unpublished
T7094 31974-31978 NNS denotes data
T7096 31978-31979 -RRB- denotes )
T7097 31979-31980 . denotes .
T7098 31980-32123 sentence denotes Escape from silencing of an Xp-inherited Atrx WT allele was also observed in the chorionic ectoderm of carrier females at 8.5 dpc (Figure S4).
T7099 31981-31987 NN denotes Escape
T7101 31988-31992 IN denotes from
T7102 31993-32002 NN denotes silencing
T7103 32003-32005 IN denotes of
T7104 32006-32008 DT denotes an
T7106 32009-32011 NN denotes Xp
T7108 32011-32012 HYPH denotes -
T7107 32012-32021 VBN denotes inherited
T7109 32022-32029 NN denotes Atrx WT
T7105 32030-32036 NN denotes allele
T7110 32037-32040 VBD denotes was
T7111 32041-32045 RB denotes also
T7100 32046-32054 VBN denotes observed
T7112 32055-32057 IN denotes in
T7113 32058-32061 DT denotes the
T7115 32062-32071 JJ denotes chorionic
T7114 32072-32080 NN denotes ectoderm
T7116 32081-32083 IN denotes of
T7117 32084-32091 NN denotes carrier
T7118 32092-32099 NNS denotes females
T7119 32100-32102 IN denotes at
T7120 32103-32106 CD denotes 8.5
T7121 32107-32110 NN denotes dpc
T7122 32111-32112 -LRB- denotes (
T7124 32112-32118 NN denotes Figure
T7123 32119-32121 NN denotes S4
T7125 32121-32122 -RRB- denotes )
T7126 32122-32123 . denotes .
T7127 32123-32352 sentence denotes Thus, although random X-inactivation occurs normally within the epiblast, the Atrx WT allele (inherited on the Xp chromosome) escaped the normal imprinted X-inactivation in the extraembryonic compartment of some carrier females.
T7128 32124-32128 RB denotes Thus
T7130 32128-32130 , denotes ,
T7131 32130-32138 IN denotes although
T7133 32139-32145 JJ denotes random
T7135 32146-32147 NN denotes X
T7136 32147-32148 HYPH denotes -
T7134 32148-32160 NN denotes inactivation
T7132 32161-32167 VBZ denotes occurs
T7137 32168-32176 RB denotes normally
T7138 32177-32183 IN denotes within
T7139 32184-32187 DT denotes the
T7140 32188-32196 NN denotes epiblast
T7141 32196-32198 , denotes ,
T7142 32198-32201 DT denotes the
T7144 32202-32209 NN denotes Atrx WT
T7143 32210-32216 NN denotes allele
T7145 32217-32218 -LRB- denotes (
T7146 32218-32227 VBN denotes inherited
T7147 32228-32230 IN denotes on
T7148 32231-32234 DT denotes the
T7150 32235-32237 NN denotes Xp
T7149 32238-32248 NN denotes chromosome
T7151 32248-32249 -RRB- denotes )
T7129 32250-32257 VBD denotes escaped
T7152 32258-32261 DT denotes the
T7154 32262-32268 JJ denotes normal
T7155 32269-32278 VBN denotes imprinted
T7156 32279-32280 NN denotes X
T7157 32280-32281 HYPH denotes -
T7153 32281-32293 NN denotes inactivation
T7158 32294-32296 IN denotes in
T7159 32297-32300 DT denotes the
T7161 32301-32315 JJ denotes extraembryonic
T7160 32316-32327 NN denotes compartment
T7162 32328-32330 IN denotes of
T7163 32331-32335 DT denotes some
T7165 32336-32343 NN denotes carrier
T7164 32344-32351 NNS denotes females
T7166 32351-32352 . denotes .
T7194 33126-33128 PRP denotes We
T7195 33129-33141 VBD denotes investigated
T7196 33142-33145 DT denotes the
T7197 33146-33150 NN denotes role
T7198 33151-33153 IN denotes of
T7199 33154-33157 DT denotes the
T7201 33158-33162 NN denotes Atrx
T7200 33163-33170 NN denotes protein
T7202 33171-33173 IN denotes in
T7203 33174-33179 NN denotes mouse
T7204 33180-33191 NN denotes development
T7205 33191-33192 . denotes .
T7206 33192-33351 sentence denotes By using a conditional knockout approach, we ablated the full-length Atrx protein first in ES cells and embryoid bodies, and then in developing mouse embryos.
T7207 33193-33195 IN denotes By
T7209 33196-33201 VBG denotes using
T7210 33202-33203 DT denotes a
T7212 33204-33215 JJ denotes conditional
T7213 33216-33224 NN denotes knockout
T7211 33225-33233 NN denotes approach
T7214 33233-33235 , denotes ,
T7215 33235-33237 PRP denotes we
T7208 33238-33245 VBD denotes ablated
T7216 33246-33249 DT denotes the
T7218 33250-33254 JJ denotes full
T7220 33254-33255 HYPH denotes -
T7219 33255-33261 NN denotes length
T7221 33262-33266 NN denotes Atrx
T7217 33267-33274 NN denotes protein
T7222 33275-33280 RB denotes first
T7223 33281-33283 IN denotes in
T7224 33284-33286 NN denotes ES
T7225 33287-33292 NNS denotes cells
T7226 33293-33296 CC denotes and
T7227 33297-33305 JJ denotes embryoid
T7228 33306-33312 NNS denotes bodies
T7229 33312-33314 , denotes ,
T7230 33314-33317 CC denotes and
T7231 33318-33322 RB denotes then
T7232 33323-33325 IN denotes in
T7233 33326-33336 VBG denotes developing
T7235 33337-33342 NN denotes mouse
T7234 33343-33350 NNS denotes embryos
T7236 33350-33351 . denotes .
T7415 33353-33357 NN denotes Atrx
T7416 33358-33360 IN denotes in
T7417 33361-33363 NN denotes ES
T7418 33364-33369 NNS denotes Cells
T7419 33369-33506 sentence denotes Atrxnull ES cells could not be recovered by direct targeting and were eventually generated by adopting a conditional targeting approach.
T7420 33370-33378 JJ denotes Atrxnull
T7421 33379-33381 NN denotes ES
T7422 33382-33387 NNS denotes cells
T7424 33388-33393 MD denotes could
T7425 33394-33397 RB denotes not
T7426 33398-33400 VB denotes be
T7423 33401-33410 VBN denotes recovered
T7427 33411-33413 IN denotes by
T7428 33414-33420 JJ denotes direct
T7429 33421-33430 NN denotes targeting
T7430 33431-33434 CC denotes and
T7431 33435-33439 VBD denotes were
T7433 33440-33450 RB denotes eventually
T7432 33451-33460 VBN denotes generated
T7434 33461-33463 IN denotes by
T7435 33464-33472 VBG denotes adopting
T7436 33473-33474 DT denotes a
T7438 33475-33486 JJ denotes conditional
T7439 33487-33496 NN denotes targeting
T7437 33497-33505 NN denotes approach
T7440 33505-33506 . denotes .
T7441 33506-33712 sentence denotes This is consistent with our observation that Atrx is highly expressed in ES cells, and that the absence of full-length Atrx imparts a growth disadvantage relative to cells bearing a functional Atrx allele.
T7442 33507-33511 DT denotes This
T7443 33512-33514 VBZ denotes is
T7444 33515-33525 JJ denotes consistent
T7445 33526-33530 IN denotes with
T7446 33531-33534 PRP$ denotes our
T7447 33535-33546 NN denotes observation
T7448 33547-33551 IN denotes that
T7450 33552-33556 NN denotes Atrx
T7449 33557-33559 VBZ denotes is
T7451 33560-33566 RB denotes highly
T7452 33567-33576 JJ denotes expressed
T7453 33577-33579 IN denotes in
T7454 33580-33582 NN denotes ES
T7455 33583-33588 NNS denotes cells
T7456 33588-33590 , denotes ,
T7457 33590-33593 CC denotes and
T7458 33594-33598 IN denotes that
T7460 33599-33602 DT denotes the
T7461 33603-33610 NN denotes absence
T7462 33611-33613 IN denotes of
T7463 33614-33618 JJ denotes full
T7465 33618-33619 HYPH denotes -
T7464 33619-33625 NN denotes length
T7466 33626-33630 NN denotes Atrx
T7459 33631-33638 VBZ denotes imparts
T7467 33639-33640 DT denotes a
T7469 33641-33647 NN denotes growth
T7468 33648-33660 NN denotes disadvantage
T7470 33661-33669 JJ denotes relative
T7471 33670-33672 IN denotes to
T7472 33673-33678 NNS denotes cells
T7473 33679-33686 VBG denotes bearing
T7474 33687-33688 DT denotes a
T7476 33689-33699 JJ denotes functional
T7477 33700-33704 NN denotes Atrx
T7475 33705-33711 NN denotes allele
T7478 33711-33712 . denotes .
T7479 33712-33796 sentence denotes At present, the cause of the proliferative delay in Atrxnull ES cells is not known.
T7480 33713-33715 IN denotes At
T7482 33716-33723 JJ denotes present
T7483 33723-33725 , denotes ,
T7484 33725-33728 DT denotes the
T7485 33729-33734 NN denotes cause
T7486 33735-33737 IN denotes of
T7487 33738-33741 DT denotes the
T7489 33742-33755 JJ denotes proliferative
T7488 33756-33761 NN denotes delay
T7490 33762-33764 IN denotes in
T7491 33765-33773 JJ denotes Atrxnull
T7493 33774-33776 NN denotes ES
T7492 33777-33782 NNS denotes cells
T7481 33783-33785 VBZ denotes is
T7494 33786-33789 RB denotes not
T7495 33790-33795 JJ denotes known
T7496 33795-33796 . denotes .
T7497 33796-33964 sentence denotes Interestingly, we demonstrated that apoptosis is not significantly up-regulated in ES cells lacking Atrx and is only mildly elevated in Atrxnull 7.5 dpc mouse embryos.
T7498 33797-33810 RB denotes Interestingly
T7500 33810-33812 , denotes ,
T7501 33812-33814 PRP denotes we
T7499 33815-33827 VBD denotes demonstrated
T7502 33828-33832 IN denotes that
T7504 33833-33842 NN denotes apoptosis
T7505 33843-33845 VBZ denotes is
T7506 33846-33849 RB denotes not
T7507 33850-33863 RB denotes significantly
T7508 33864-33866 RB denotes up
T7509 33866-33867 HYPH denotes -
T7503 33867-33876 VBN denotes regulated
T7510 33877-33879 IN denotes in
T7511 33880-33882 NN denotes ES
T7512 33883-33888 NNS denotes cells
T7513 33889-33896 VBG denotes lacking
T7514 33897-33901 NN denotes Atrx
T7515 33902-33905 CC denotes and
T7516 33906-33908 VBZ denotes is
T7517 33909-33913 RB denotes only
T7518 33914-33920 RB denotes mildly
T7519 33921-33929 JJ denotes elevated
T7520 33930-33932 IN denotes in
T7521 33933-33941 JJ denotes Atrxnull
T7523 33942-33945 CD denotes 7.5
T7524 33946-33949 NN denotes dpc
T7525 33950-33955 NN denotes mouse
T7522 33956-33963 NNS denotes embryos
T7526 33963-33964 . denotes .
T7527 33964-34260 sentence denotes In contrast, it was recently shown that the loss of Atrx markedly increased the apoptotic population in the differentiating cells of the embryonic cortex and postnatal hippocampus, when Atrx expression was ablated in the developing mouse forebrain using the Atrx flox allele described here [20].
T7528 33965-33967 IN denotes In
T7530 33968-33976 NN denotes contrast
T7531 33976-33978 , denotes ,
T7532 33978-33980 PRP denotes it
T7533 33981-33984 VBD denotes was
T7534 33985-33993 RB denotes recently
T7529 33994-33999 VBN denotes shown
T7535 34000-34004 IN denotes that
T7537 34005-34008 DT denotes the
T7538 34009-34013 NN denotes loss
T7539 34014-34016 IN denotes of
T7540 34017-34021 NN denotes Atrx
T7541 34022-34030 RB denotes markedly
T7536 34031-34040 VBD denotes increased
T7542 34041-34044 DT denotes the
T7544 34045-34054 JJ denotes apoptotic
T7543 34055-34065 NN denotes population
T7545 34066-34068 IN denotes in
T7546 34069-34072 DT denotes the
T7548 34073-34088 VBG denotes differentiating
T7547 34089-34094 NNS denotes cells
T7549 34095-34097 IN denotes of
T7550 34098-34101 DT denotes the
T7552 34102-34111 JJ denotes embryonic
T7551 34112-34118 NN denotes cortex
T7553 34119-34122 CC denotes and
T7554 34123-34132 JJ denotes postnatal
T7555 34133-34144 NN denotes hippocampus
T7556 34144-34146 , denotes ,
T7557 34146-34150 WRB denotes when
T7559 34151-34155 NN denotes Atrx
T7560 34156-34166 NN denotes expression
T7561 34167-34170 VBD denotes was
T7558 34171-34178 VBN denotes ablated
T7562 34179-34181 IN denotes in
T7563 34182-34185 DT denotes the
T7565 34186-34196 VBG denotes developing
T7566 34197-34202 NN denotes mouse
T7564 34203-34212 NN denotes forebrain
T7567 34213-34218 VBG denotes using
T7568 34219-34222 DT denotes the
T7570 34223-34232 NN denotes Atrx flox
T7569 34233-34239 NN denotes allele
T7571 34240-34249 VBN denotes described
T7572 34250-34254 RB denotes here
T7573 34255-34256 -LRB- denotes [
T7574 34256-34258 CD denotes 20
T7575 34258-34259 -RRB- denotes ]
T7576 34259-34260 . denotes .
T7577 34260-34433 sentence denotes The human ATRX protein has been shown to associate in a complex with Daxx [8], a protein that has been implicated in multiple pathways for the regulation of apoptosis [21].
T7578 34261-34264 DT denotes The
T7580 34265-34270 JJ denotes human
T7581 34271-34275 NN denotes ATRX
T7579 34276-34283 NN denotes protein
T7583 34284-34287 VBZ denotes has
T7584 34288-34292 VBN denotes been
T7582 34293-34298 VBN denotes shown
T7585 34299-34301 TO denotes to
T7586 34302-34311 VB denotes associate
T7587 34312-34314 IN denotes in
T7588 34315-34316 DT denotes a
T7589 34317-34324 NN denotes complex
T7590 34325-34329 IN denotes with
T7591 34330-34334 NN denotes Daxx
T7592 34335-34336 -LRB- denotes [
T7593 34336-34337 CD denotes 8
T7594 34337-34338 -RRB- denotes ]
T7595 34338-34340 , denotes ,
T7596 34340-34341 DT denotes a
T7597 34342-34349 NN denotes protein
T7598 34350-34354 WDT denotes that
T7600 34355-34358 VBZ denotes has
T7601 34359-34363 VBN denotes been
T7599 34364-34374 VBN denotes implicated
T7602 34375-34377 IN denotes in
T7603 34378-34386 JJ denotes multiple
T7604 34387-34395 NNS denotes pathways
T7605 34396-34399 IN denotes for
T7606 34400-34403 DT denotes the
T7607 34404-34414 NN denotes regulation
T7608 34415-34417 IN denotes of
T7609 34418-34427 NN denotes apoptosis
T7610 34428-34429 -LRB- denotes [
T7611 34429-34431 CD denotes 21
T7612 34431-34432 -RRB- denotes ]
T7613 34432-34433 . denotes .
T7614 34433-34585 sentence denotes It is possible that disruption of the mouse Atrx-Daxx complex (by ablation of the Atrx protein) could have triggered a universal proapoptotic response.
T7615 34434-34436 PRP denotes It
T7616 34437-34439 VBZ denotes is
T7617 34440-34448 JJ denotes possible
T7618 34449-34453 IN denotes that
T7620 34454-34464 NN denotes disruption
T7621 34465-34467 IN denotes of
T7622 34468-34471 DT denotes the
T7624 34472-34477 NN denotes mouse
T7625 34478-34482 NN denotes Atrx
T7627 34482-34483 HYPH denotes -
T7626 34483-34487 NN denotes Daxx
T7623 34488-34495 NN denotes complex
T7628 34496-34497 -LRB- denotes (
T7629 34497-34499 IN denotes by
T7630 34500-34508 NN denotes ablation
T7631 34509-34511 IN denotes of
T7632 34512-34515 DT denotes the
T7634 34516-34520 NN denotes Atrx
T7633 34521-34528 NN denotes protein
T7635 34528-34529 -RRB- denotes )
T7636 34530-34535 MD denotes could
T7637 34536-34540 VB denotes have
T7619 34541-34550 VBN denotes triggered
T7638 34551-34552 DT denotes a
T7640 34553-34562 JJ denotes universal
T7641 34563-34575 JJ denotes proapoptotic
T7639 34576-34584 NN denotes response
T7642 34584-34585 . denotes .
T7643 34585-34899 sentence denotes However, our observations in ES cells demonstrate that the induction of apoptosis is not an automatic response triggered by the removal of Atrx in all cell types, and suggest that the inappropriate apoptosis observed in the Atrx-mutant forebrain may reflect a requirement for Atrx during terminal differentiation.
T7644 34586-34593 RB denotes However
T7646 34593-34595 , denotes ,
T7647 34595-34598 PRP$ denotes our
T7648 34599-34611 NNS denotes observations
T7649 34612-34614 IN denotes in
T7650 34615-34617 NN denotes ES
T7651 34618-34623 NNS denotes cells
T7645 34624-34635 VBP denotes demonstrate
T7652 34636-34640 IN denotes that
T7654 34641-34644 DT denotes the
T7655 34645-34654 NN denotes induction
T7656 34655-34657 IN denotes of
T7657 34658-34667 NN denotes apoptosis
T7653 34668-34670 VBZ denotes is
T7658 34671-34674 RB denotes not
T7659 34675-34677 DT denotes an
T7661 34678-34687 JJ denotes automatic
T7660 34688-34696 NN denotes response
T7662 34697-34706 VBN denotes triggered
T7663 34707-34709 IN denotes by
T7664 34710-34713 DT denotes the
T7665 34714-34721 NN denotes removal
T7666 34722-34724 IN denotes of
T7667 34725-34729 NN denotes Atrx
T7668 34730-34732 IN denotes in
T7669 34733-34736 DT denotes all
T7671 34737-34741 NN denotes cell
T7670 34742-34747 NNS denotes types
T7672 34747-34749 , denotes ,
T7673 34749-34752 CC denotes and
T7674 34753-34760 VBP denotes suggest
T7675 34761-34765 IN denotes that
T7677 34766-34769 DT denotes the
T7679 34770-34783 JJ denotes inappropriate
T7678 34784-34793 NN denotes apoptosis
T7680 34794-34802 VBN denotes observed
T7681 34803-34805 IN denotes in
T7682 34806-34809 DT denotes the
T7684 34810-34814 NN denotes Atrx
T7686 34814-34815 HYPH denotes -
T7685 34815-34821 NN denotes mutant
T7683 34822-34831 NN denotes forebrain
T7687 34832-34835 MD denotes may
T7676 34836-34843 VB denotes reflect
T7688 34844-34845 DT denotes a
T7689 34846-34857 NN denotes requirement
T7690 34858-34861 IN denotes for
T7691 34862-34866 NN denotes Atrx
T7692 34867-34873 IN denotes during
T7693 34874-34882 JJ denotes terminal
T7694 34883-34898 NN denotes differentiation
T7695 34898-34899 . denotes .
T8323 34901-34903 DT denotes An
T8325 34904-34914 JJ denotes Unexpected
T8324 34915-34919 NN denotes Role
T8326 34920-34923 IN denotes for
T8327 34924-34928 NN denotes Atrx
T8328 34929-34931 IN denotes in
T8329 34932-34943 NN denotes Development
T8330 34944-34946 IN denotes of
T8331 34947-34950 DT denotes the
T8333 34951-34956 NN denotes Mouse
T8332 34957-34968 NN denotes Trophoblast
T8334 34968-35050 sentence denotes We show that Atrxnull male mice are not viable and embryos die by around 9.5 dpc.
T8335 34969-34971 PRP denotes We
T8336 34972-34976 VBP denotes show
T8337 34977-34981 IN denotes that
T8339 34982-34990 JJ denotes Atrxnull
T8341 34991-34995 JJ denotes male
T8340 34996-35000 NNS denotes mice
T8338 35001-35004 VBP denotes are
T8342 35005-35008 RB denotes not
T8343 35009-35015 JJ denotes viable
T8344 35016-35019 CC denotes and
T8345 35020-35027 NNS denotes embryos
T8346 35028-35031 VBP denotes die
T8347 35032-35034 IN denotes by
T8348 35035-35041 IN denotes around
T8349 35042-35045 CD denotes 9.5
T8350 35046-35049 NN denotes dpc
T8351 35049-35050 . denotes .
T8352 35050-35158 sentence denotes Before death, Atrxnull embryos exhibit a markedly reduced mitotic index, suggesting a proliferative defect.
T8353 35051-35057 IN denotes Before
T8355 35058-35063 NN denotes death
T8356 35063-35065 , denotes ,
T8357 35065-35073 JJ denotes Atrxnull
T8358 35074-35081 NNS denotes embryos
T8354 35082-35089 VBP denotes exhibit
T8359 35090-35091 DT denotes a
T8361 35092-35100 RB denotes markedly
T8362 35101-35108 VBN denotes reduced
T8363 35109-35116 JJ denotes mitotic
T8360 35117-35122 NN denotes index
T8364 35122-35124 , denotes ,
T8365 35124-35134 VBG denotes suggesting
T8366 35135-35136 DT denotes a
T8368 35137-35150 JJ denotes proliferative
T8367 35151-35157 NN denotes defect
T8369 35157-35158 . denotes .
T8370 35158-35505 sentence denotes Although the embryonic compartment of the conceptus appears initially normal, by 7.5 dpc Atrxnull embryos display abnormalities in development of the trophoblast, including a depletion in the population of TGCs surrounding the conceptus and a reduction in the size of the ectoplacental cone, which contains the diploid giant cell precursors [22].
T8371 35159-35167 IN denotes Although
T8373 35168-35171 DT denotes the
T8375 35172-35181 JJ denotes embryonic
T8374 35182-35193 NN denotes compartment
T8376 35194-35196 IN denotes of
T8377 35197-35200 DT denotes the
T8378 35201-35210 NN denotes conceptus
T8372 35211-35218 VBZ denotes appears
T8380 35219-35228 RB denotes initially
T8381 35229-35235 JJ denotes normal
T8382 35235-35237 , denotes ,
T8383 35237-35239 IN denotes by
T8384 35240-35243 CD denotes 7.5
T8385 35244-35247 NN denotes dpc
T8386 35248-35256 JJ denotes Atrxnull
T8387 35257-35264 NNS denotes embryos
T8379 35265-35272 VBP denotes display
T8388 35273-35286 NNS denotes abnormalities
T8389 35287-35289 IN denotes in
T8390 35290-35301 NN denotes development
T8391 35302-35304 IN denotes of
T8392 35305-35308 DT denotes the
T8393 35309-35320 NN denotes trophoblast
T8394 35320-35322 , denotes ,
T8395 35322-35331 VBG denotes including
T8396 35332-35333 DT denotes a
T8397 35334-35343 NN denotes depletion
T8398 35344-35346 IN denotes in
T8399 35347-35350 DT denotes the
T8400 35351-35361 NN denotes population
T8401 35362-35364 IN denotes of
T8402 35365-35369 NNS denotes TGCs
T8403 35370-35381 VBG denotes surrounding
T8404 35382-35385 DT denotes the
T8405 35386-35395 NN denotes conceptus
T8406 35396-35399 CC denotes and
T8407 35400-35401 DT denotes a
T8408 35402-35411 NN denotes reduction
T8409 35412-35414 IN denotes in
T8410 35415-35418 DT denotes the
T8411 35419-35423 NN denotes size
T8412 35424-35426 IN denotes of
T8413 35427-35430 DT denotes the
T8415 35431-35444 JJ denotes ectoplacental
T8414 35445-35449 NN denotes cone
T8416 35449-35451 , denotes ,
T8417 35451-35456 WDT denotes which
T8418 35457-35465 VBZ denotes contains
T8419 35466-35469 DT denotes the
T8421 35470-35477 NN denotes diploid
T8422 35478-35483 JJ denotes giant
T8423 35484-35488 NN denotes cell
T8420 35489-35499 NNS denotes precursors
T8424 35500-35501 -LRB- denotes [
T8425 35501-35503 CD denotes 22
T8426 35503-35504 -RRB- denotes ]
T8427 35504-35505 . denotes .
T8428 35505-35692 sentence denotes TGCs are highly differentiated, postmitotic cells that ultimately form an epithelial layer at the periphery of the placenta, which interfaces with the maternal cells of the decidua [23].
T8429 35506-35510 NNS denotes TGCs
T8430 35511-35514 VBP denotes are
T8431 35515-35521 RB denotes highly
T8432 35522-35536 VBN denotes differentiated
T8434 35536-35538 , denotes ,
T8435 35538-35549 JJ denotes postmitotic
T8433 35550-35555 NNS denotes cells
T8436 35556-35560 WDT denotes that
T8438 35561-35571 RB denotes ultimately
T8437 35572-35576 VBP denotes form
T8439 35577-35579 DT denotes an
T8441 35580-35590 JJ denotes epithelial
T8440 35591-35596 NN denotes layer
T8442 35597-35599 IN denotes at
T8443 35600-35603 DT denotes the
T8444 35604-35613 NN denotes periphery
T8445 35614-35616 IN denotes of
T8446 35617-35620 DT denotes the
T8447 35621-35629 NN denotes placenta
T8448 35629-35631 , denotes ,
T8449 35631-35636 WDT denotes which
T8450 35637-35647 VBZ denotes interfaces
T8451 35648-35652 IN denotes with
T8452 35653-35656 DT denotes the
T8454 35657-35665 JJ denotes maternal
T8453 35666-35671 NNS denotes cells
T8455 35672-35674 IN denotes of
T8456 35675-35678 DT denotes the
T8457 35679-35686 NN denotes decidua
T8458 35687-35688 -LRB- denotes [
T8459 35688-35690 CD denotes 23
T8460 35690-35691 -RRB- denotes ]
T8461 35691-35692 . denotes .
T8462 35692-35937 sentence denotes These highly invasive cells are important for mediating initial invasion of the uterine tissue, but are also involved in remodelling the maternal decidua after implantation and in secreting hormones that regulate fetal and maternal growth [24].
T8463 35693-35698 DT denotes These
T8465 35699-35705 RB denotes highly
T8466 35706-35714 JJ denotes invasive
T8464 35715-35720 NNS denotes cells
T8467 35721-35724 VBP denotes are
T8468 35725-35734 JJ denotes important
T8469 35735-35738 IN denotes for
T8470 35739-35748 VBG denotes mediating
T8471 35749-35756 JJ denotes initial
T8472 35757-35765 NN denotes invasion
T8473 35766-35768 IN denotes of
T8474 35769-35772 DT denotes the
T8476 35773-35780 JJ denotes uterine
T8475 35781-35787 NN denotes tissue
T8477 35787-35789 , denotes ,
T8478 35789-35792 CC denotes but
T8479 35793-35796 VBP denotes are
T8481 35797-35801 RB denotes also
T8480 35802-35810 VBN denotes involved
T8482 35811-35813 IN denotes in
T8483 35814-35825 VBG denotes remodelling
T8484 35826-35829 DT denotes the
T8486 35830-35838 JJ denotes maternal
T8485 35839-35846 NN denotes decidua
T8487 35847-35852 IN denotes after
T8488 35853-35865 NN denotes implantation
T8489 35866-35869 CC denotes and
T8490 35870-35872 IN denotes in
T8491 35873-35882 VBG denotes secreting
T8492 35883-35891 NNS denotes hormones
T8493 35892-35896 WDT denotes that
T8494 35897-35905 VBP denotes regulate
T8495 35906-35911 JJ denotes fetal
T8497 35912-35915 CC denotes and
T8498 35916-35924 JJ denotes maternal
T8496 35925-35931 NN denotes growth
T8499 35932-35933 -LRB- denotes [
T8500 35933-35935 CD denotes 24
T8501 35935-35936 -RRB- denotes ]
T8502 35936-35937 . denotes .
T8503 35937-36076 sentence denotes Since Atrxnull embryos appear to implant normally, lethality is likely to arise due to a failure of TGC function later during development.
T8504 35938-35943 IN denotes Since
T8506 35944-35952 JJ denotes Atrxnull
T8507 35953-35960 NNS denotes embryos
T8505 35961-35967 VBP denotes appear
T8509 35968-35970 TO denotes to
T8510 35971-35978 VB denotes implant
T8511 35979-35987 RB denotes normally
T8512 35987-35989 , denotes ,
T8513 35989-35998 NN denotes lethality
T8508 35999-36001 VBZ denotes is
T8514 36002-36008 JJ denotes likely
T8515 36009-36011 TO denotes to
T8516 36012-36017 VB denotes arise
T8517 36018-36021 IN denotes due
T8518 36022-36024 IN denotes to
T8519 36025-36026 DT denotes a
T8520 36027-36034 NN denotes failure
T8521 36035-36037 IN denotes of
T8522 36038-36041 NN denotes TGC
T8523 36042-36050 NN denotes function
T8524 36051-36056 RB denotes later
T8525 36057-36063 IN denotes during
T8526 36064-36075 NN denotes development
T8527 36075-36076 . denotes .
T8528 36076-36229 sentence denotes Embryonic lethality in mice in the absence of Atrx was a surprising finding, as there had been no suggestion of foetal loss in the human ATR-X syndrome.
T8529 36077-36086 JJ denotes Embryonic
T8530 36087-36096 NN denotes lethality
T8532 36097-36099 IN denotes in
T8533 36100-36104 NNS denotes mice
T8534 36105-36107 IN denotes in
T8535 36108-36111 DT denotes the
T8536 36112-36119 NN denotes absence
T8537 36120-36122 IN denotes of
T8538 36123-36127 NN denotes Atrx
T8531 36128-36131 VBD denotes was
T8539 36132-36133 DT denotes a
T8541 36134-36144 JJ denotes surprising
T8540 36145-36152 NN denotes finding
T8542 36152-36154 , denotes ,
T8543 36154-36156 IN denotes as
T8545 36157-36162 EX denotes there
T8546 36163-36166 VBD denotes had
T8544 36167-36171 VBN denotes been
T8547 36172-36174 DT denotes no
T8548 36175-36185 NN denotes suggestion
T8549 36186-36188 IN denotes of
T8550 36189-36195 JJ denotes foetal
T8551 36196-36200 NN denotes loss
T8552 36201-36203 IN denotes in
T8553 36204-36207 DT denotes the
T8555 36208-36213 JJ denotes human
T8556 36214-36217 NN denotes ATR
T8558 36217-36218 HYPH denotes -
T8557 36218-36219 NN denotes X
T8554 36220-36228 NN denotes syndrome
T8559 36228-36229 . denotes .
T8560 36229-36373 sentence denotes It is possible that the role of Atrx in the trophoblast is specific to mice and that ATRX has no role or is redundant in the human trophoblast.
T8561 36230-36232 PRP denotes It
T8562 36233-36235 VBZ denotes is
T8563 36236-36244 JJ denotes possible
T8564 36245-36249 IN denotes that
T8566 36250-36253 DT denotes the
T8567 36254-36258 NN denotes role
T8568 36259-36261 IN denotes of
T8569 36262-36266 NN denotes Atrx
T8570 36267-36269 IN denotes in
T8571 36270-36273 DT denotes the
T8572 36274-36285 NN denotes trophoblast
T8565 36286-36288 VBZ denotes is
T8573 36289-36297 JJ denotes specific
T8574 36298-36300 IN denotes to
T8575 36301-36305 NNS denotes mice
T8576 36306-36309 CC denotes and
T8577 36310-36314 IN denotes that
T8579 36315-36319 NN denotes ATRX
T8578 36320-36323 VBZ denotes has
T8580 36324-36326 DT denotes no
T8581 36327-36331 NN denotes role
T8582 36332-36334 CC denotes or
T8583 36335-36337 VBZ denotes is
T8584 36338-36347 JJ denotes redundant
T8585 36348-36350 IN denotes in
T8586 36351-36354 DT denotes the
T8588 36355-36360 JJ denotes human
T8587 36361-36372 NN denotes trophoblast
T8589 36372-36373 . denotes .
T8590 36373-36447 sentence denotes Indeed, the birth weight of babies with ATR-X syndrome is usually normal.
T8591 36374-36380 RB denotes Indeed
T8593 36380-36382 , denotes ,
T8594 36382-36385 DT denotes the
T8596 36386-36391 NN denotes birth
T8595 36392-36398 NN denotes weight
T8597 36399-36401 IN denotes of
T8598 36402-36408 NNS denotes babies
T8599 36409-36413 IN denotes with
T8600 36414-36417 NN denotes ATR
T8602 36417-36418 HYPH denotes -
T8601 36418-36419 NN denotes X
T8603 36420-36428 NN denotes syndrome
T8592 36429-36431 VBZ denotes is
T8604 36432-36439 RB denotes usually
T8605 36440-36446 JJ denotes normal
T8606 36446-36447 . denotes .
T8607 36447-36654 sentence denotes An alternative explanation for the unexpectedly severe phenotype we observed in mice is that the Atrx Δ18Δneo deletion generated by Cre recombination completely ablates full-length Atrx protein (Figure 2E).
T8608 36448-36450 DT denotes An
T8610 36451-36462 JJ denotes alternative
T8609 36463-36474 NN denotes explanation
T8612 36475-36478 IN denotes for
T8613 36479-36482 DT denotes the
T8615 36483-36495 RB denotes unexpectedly
T8616 36496-36502 JJ denotes severe
T8614 36503-36512 NN denotes phenotype
T8617 36513-36515 PRP denotes we
T8618 36516-36524 VBD denotes observed
T8619 36525-36527 IN denotes in
T8620 36528-36532 NNS denotes mice
T8611 36533-36535 VBZ denotes is
T8621 36536-36540 IN denotes that
T8623 36541-36544 DT denotes the
T8625 36545-36557 NN denotes Atrx Δ18Δneo
T8624 36558-36566 NN denotes deletion
T8626 36567-36576 VBN denotes generated
T8627 36577-36579 IN denotes by
T8628 36580-36583 NN denotes Cre
T8629 36584-36597 NN denotes recombination
T8630 36598-36608 RB denotes completely
T8622 36609-36616 VBZ denotes ablates
T8631 36617-36621 JJ denotes full
T8633 36621-36622 HYPH denotes -
T8632 36622-36628 NN denotes length
T8635 36629-36633 NN denotes Atrx
T8634 36634-36641 NN denotes protein
T8636 36642-36643 -LRB- denotes (
T8638 36643-36649 NN denotes Figure
T8637 36650-36652 NN denotes 2E
T8639 36652-36653 -RRB- denotes )
T8640 36653-36654 . denotes .
T8641 36654-36780 sentence denotes In contrast, all disease-causing mutations characterised in human ATR-X pedigrees appear to give rise to hypomorphic alleles.
T8642 36655-36657 IN denotes In
T8644 36658-36666 NN denotes contrast
T8645 36666-36668 , denotes ,
T8646 36668-36671 DT denotes all
T8648 36672-36679 NN denotes disease
T8650 36679-36680 HYPH denotes -
T8649 36680-36687 VBG denotes causing
T8647 36688-36697 NNS denotes mutations
T8651 36698-36711 VBN denotes characterised
T8652 36712-36714 IN denotes in
T8653 36715-36720 JJ denotes human
T8655 36721-36724 NN denotes ATR
T8657 36724-36725 HYPH denotes -
T8656 36725-36726 NN denotes X
T8654 36727-36736 NNS denotes pedigrees
T8643 36737-36743 VBP denotes appear
T8658 36744-36746 TO denotes to
T8659 36747-36751 VB denotes give
T8660 36752-36756 NN denotes rise
T8661 36757-36759 IN denotes to
T8662 36760-36771 JJ denotes hypomorphic
T8663 36772-36779 NNS denotes alleles
T8664 36779-36780 . denotes .
T8665 36780-37100 sentence denotes Some full-length ATRX protein is detected in cases predicted to have truncating mutations (RJG, unpublished data), and residual ATPase activity in ATRX immunoprecipitates can be detected in Epstein-Barr virus-transformed lymphocytes of all human patients analysed to date (A. Argentaro and M. Mitson, unpublished data).
T8666 36781-36785 DT denotes Some
T8668 36786-36790 JJ denotes full
T8670 36790-36791 HYPH denotes -
T8669 36791-36797 NN denotes length
T8671 36798-36802 NN denotes ATRX
T8667 36803-36810 NN denotes protein
T8673 36811-36813 VBZ denotes is
T8672 36814-36822 VBN denotes detected
T8674 36823-36825 IN denotes in
T8675 36826-36831 NNS denotes cases
T8676 36832-36841 VBN denotes predicted
T8677 36842-36844 TO denotes to
T8678 36845-36849 VB denotes have
T8679 36850-36860 NN denotes truncating
T8680 36861-36870 NNS denotes mutations
T8681 36871-36872 -LRB- denotes (
T8683 36872-36875 NN denotes RJG
T8684 36875-36877 , denotes ,
T8685 36877-36888 JJ denotes unpublished
T8682 36889-36893 NNS denotes data
T8686 36893-36894 -RRB- denotes )
T8687 36894-36896 , denotes ,
T8688 36896-36899 CC denotes and
T8689 36900-36908 JJ denotes residual
T8691 36909-36915 NN denotes ATPase
T8690 36916-36924 NN denotes activity
T8693 36925-36927 IN denotes in
T8694 36928-36932 NN denotes ATRX
T8695 36933-36951 NNS denotes immunoprecipitates
T8696 36952-36955 MD denotes can
T8697 36956-36958 VB denotes be
T8692 36959-36967 VBN denotes detected
T8698 36968-36970 IN denotes in
T8699 36971-36978 NN denotes Epstein
T8701 36978-36979 HYPH denotes -
T8700 36979-36983 NN denotes Barr
T8702 36984-36989 NN denotes virus
T8704 36989-36990 HYPH denotes -
T8703 36990-37001 VBN denotes transformed
T8705 37002-37013 NNS denotes lymphocytes
T8706 37014-37016 IN denotes of
T8707 37017-37020 DT denotes all
T8709 37021-37026 JJ denotes human
T8708 37027-37035 NNS denotes patients
T8710 37036-37044 VBN denotes analysed
T8711 37045-37047 IN denotes to
T8712 37048-37052 NN denotes date
T8713 37053-37054 -LRB- denotes (
T8714 37054-37056 NNP denotes A.
T8715 37057-37066 NNP denotes Argentaro
T8716 37067-37070 CC denotes and
T8717 37071-37073 NNP denotes M.
T8718 37074-37080 NNP denotes Mitson
T8719 37080-37082 , denotes ,
T8720 37082-37093 JJ denotes unpublished
T8721 37094-37098 NNS denotes data
T8722 37098-37099 -RRB- denotes )
T8723 37099-37100 . denotes .
T8724 37100-37290 sentence denotes The failure to observe a truly null ATRX allele among human patients strongly suggests that, as in the mouse, the complete absence of ATRX protein is incompatible with human fetal survival.
T8725 37101-37104 DT denotes The
T8726 37105-37112 NN denotes failure
T8728 37113-37115 TO denotes to
T8729 37116-37123 VB denotes observe
T8730 37124-37125 DT denotes a
T8732 37126-37131 RB denotes truly
T8733 37132-37136 JJ denotes null
T8734 37137-37141 NN denotes ATRX
T8731 37142-37148 NN denotes allele
T8735 37149-37154 IN denotes among
T8736 37155-37160 JJ denotes human
T8737 37161-37169 NNS denotes patients
T8738 37170-37178 RB denotes strongly
T8727 37179-37187 VBZ denotes suggests
T8739 37188-37192 IN denotes that
T8741 37192-37194 , denotes ,
T8742 37194-37196 IN denotes as
T8743 37197-37199 IN denotes in
T8744 37200-37203 DT denotes the
T8745 37204-37209 NN denotes mouse
T8746 37209-37211 , denotes ,
T8747 37211-37214 DT denotes the
T8749 37215-37223 JJ denotes complete
T8748 37224-37231 NN denotes absence
T8750 37232-37234 IN denotes of
T8751 37235-37239 NN denotes ATRX
T8752 37240-37247 NN denotes protein
T8740 37248-37250 VBZ denotes is
T8753 37251-37263 JJ denotes incompatible
T8754 37264-37268 IN denotes with
T8755 37269-37274 JJ denotes human
T8757 37275-37280 JJ denotes fetal
T8756 37281-37289 NN denotes survival
T8758 37289-37290 . denotes .
T8759 37290-37556 sentence denotes While this study has revealed an unexpected role for Atrx in the murine trophectoderm, as a result of the early lethality observed in Atrxnull embryos it is not possible to rule out other roles for Atrx at later developmental stages in tissues of the embryo proper.
T8760 37291-37296 IN denotes While
T8762 37297-37301 DT denotes this
T8763 37302-37307 NN denotes study
T8764 37308-37311 VBZ denotes has
T8761 37312-37320 VBN denotes revealed
T8766 37321-37323 DT denotes an
T8768 37324-37334 JJ denotes unexpected
T8767 37335-37339 NN denotes role
T8769 37340-37343 IN denotes for
T8770 37344-37348 NN denotes Atrx
T8771 37349-37351 IN denotes in
T8772 37352-37355 DT denotes the
T8774 37356-37362 JJ denotes murine
T8773 37363-37376 NN denotes trophectoderm
T8775 37376-37378 , denotes ,
T8776 37378-37380 IN denotes as
T8777 37381-37382 DT denotes a
T8778 37383-37389 NN denotes result
T8779 37390-37392 IN denotes of
T8780 37393-37396 DT denotes the
T8782 37397-37402 JJ denotes early
T8781 37403-37412 NN denotes lethality
T8783 37413-37421 VBN denotes observed
T8784 37422-37424 IN denotes in
T8785 37425-37433 JJ denotes Atrxnull
T8786 37434-37441 NNS denotes embryos
T8787 37442-37444 PRP denotes it
T8765 37445-37447 VBZ denotes is
T8788 37448-37451 RB denotes not
T8789 37452-37460 JJ denotes possible
T8790 37461-37463 TO denotes to
T8791 37464-37468 VB denotes rule
T8792 37469-37472 RP denotes out
T8793 37473-37478 JJ denotes other
T8794 37479-37484 NNS denotes roles
T8795 37485-37488 IN denotes for
T8796 37489-37493 NN denotes Atrx
T8797 37494-37496 IN denotes at
T8798 37497-37502 JJ denotes later
T8800 37503-37516 JJ denotes developmental
T8799 37517-37523 NNS denotes stages
T8801 37524-37526 IN denotes in
T8802 37527-37534 NNS denotes tissues
T8803 37535-37537 IN denotes of
T8804 37538-37541 DT denotes the
T8805 37542-37548 NN denotes embryo
T8806 37549-37555 JJ denotes proper
T8807 37555-37556 . denotes .
T8808 37556-37767 sentence denotes Indeed, we show that Atrx is highly expressed throughout the entire developing embryo at 7.5 dpc (Figure 5B), and it is likely that Atrx function will turn out to be important for other differentiating tissues.
T8809 37557-37563 RB denotes Indeed
T8811 37563-37565 , denotes ,
T8812 37565-37567 PRP denotes we
T8810 37568-37572 VBP denotes show
T8813 37573-37577 IN denotes that
T8815 37578-37582 NN denotes Atrx
T8814 37583-37585 VBZ denotes is
T8816 37586-37592 RB denotes highly
T8817 37593-37602 JJ denotes expressed
T8818 37603-37613 IN denotes throughout
T8819 37614-37617 DT denotes the
T8821 37618-37624 JJ denotes entire
T8822 37625-37635 VBG denotes developing
T8820 37636-37642 NN denotes embryo
T8823 37643-37645 IN denotes at
T8824 37646-37649 CD denotes 7.5
T8825 37650-37653 NN denotes dpc
T8826 37654-37655 -LRB- denotes (
T8828 37655-37661 NN denotes Figure
T8827 37662-37664 NN denotes 5B
T8829 37664-37665 -RRB- denotes )
T8830 37665-37667 , denotes ,
T8831 37667-37670 CC denotes and
T8832 37671-37673 PRP denotes it
T8833 37674-37676 VBZ denotes is
T8834 37677-37683 JJ denotes likely
T8835 37684-37688 IN denotes that
T8837 37689-37693 NN denotes Atrx
T8838 37694-37702 NN denotes function
T8839 37703-37707 MD denotes will
T8836 37708-37712 VB denotes turn
T8840 37713-37716 RP denotes out
T8841 37717-37719 TO denotes to
T8842 37720-37722 VB denotes be
T8843 37723-37732 JJ denotes important
T8844 37733-37736 IN denotes for
T8845 37737-37742 JJ denotes other
T8847 37743-37758 VBG denotes differentiating
T8846 37759-37766 NNS denotes tissues
T8848 37766-37767 . denotes .
T8849 37767-38114 sentence denotes Tetraploid aggregation experiments (in which mutant embryos are rescued with wild-type extraembryonic tissues) might shed more light on the role of Atrx during later mouse development, but these issues can be more subtly dissected by combining the conditional Atrx flox allele that we have generated with different tissue-specific Cre transgenes.
T8850 37768-37778 NN denotes Tetraploid
T8851 37779-37790 NN denotes aggregation
T8852 37791-37802 NNS denotes experiments
T8854 37803-37804 -LRB- denotes (
T8855 37804-37806 IN denotes in
T8857 37807-37812 WDT denotes which
T8858 37813-37819 NN denotes mutant
T8859 37820-37827 NNS denotes embryos
T8860 37828-37831 VBP denotes are
T8856 37832-37839 VBN denotes rescued
T8861 37840-37844 IN denotes with
T8862 37845-37849 JJ denotes wild
T8864 37849-37850 HYPH denotes -
T8863 37850-37854 NN denotes type
T8866 37855-37869 JJ denotes extraembryonic
T8865 37870-37877 NNS denotes tissues
T8867 37877-37878 -RRB- denotes )
T8868 37879-37884 MD denotes might
T8853 37885-37889 VB denotes shed
T8869 37890-37894 JJR denotes more
T8870 37895-37900 NN denotes light
T8871 37901-37903 IN denotes on
T8872 37904-37907 DT denotes the
T8873 37908-37912 NN denotes role
T8874 37913-37915 IN denotes of
T8875 37916-37920 NN denotes Atrx
T8876 37921-37927 IN denotes during
T8877 37928-37933 JJ denotes later
T8879 37934-37939 NN denotes mouse
T8878 37940-37951 NN denotes development
T8880 37951-37953 , denotes ,
T8881 37953-37956 CC denotes but
T8882 37957-37962 DT denotes these
T8883 37963-37969 NNS denotes issues
T8885 37970-37973 MD denotes can
T8886 37974-37976 VB denotes be
T8887 37977-37981 RBR denotes more
T8888 37982-37988 RB denotes subtly
T8884 37989-37998 VBN denotes dissected
T8889 37999-38001 IN denotes by
T8890 38002-38011 VBG denotes combining
T8891 38012-38015 DT denotes the
T8893 38016-38027 JJ denotes conditional
T8894 38028-38037 NN denotes Atrx flox
T8892 38038-38044 NN denotes allele
T8895 38045-38049 WDT denotes that
T8897 38050-38052 PRP denotes we
T8898 38053-38057 VBP denotes have
T8896 38058-38067 VBN denotes generated
T8899 38068-38072 IN denotes with
T8900 38073-38082 JJ denotes different
T8902 38083-38089 NN denotes tissue
T8904 38089-38090 HYPH denotes -
T8903 38090-38098 JJ denotes specific
T8905 38099-38102 NN denotes Cre
T8901 38103-38113 NNS denotes transgenes
T8906 38113-38114 . denotes .
T8907 38114-38246 sentence denotes As mentioned above, this approach has already revealed a critical role for Atrx during neuronal differentiation in adult mice [20].
T8908 38115-38117 IN denotes As
T8909 38118-38127 VBN denotes mentioned
T8911 38128-38133 RB denotes above
T8912 38133-38135 , denotes ,
T8913 38135-38139 DT denotes this
T8914 38140-38148 NN denotes approach
T8915 38149-38152 VBZ denotes has
T8916 38153-38160 RB denotes already
T8910 38161-38169 VBN denotes revealed
T8917 38170-38171 DT denotes a
T8919 38172-38180 JJ denotes critical
T8918 38181-38185 NN denotes role
T8920 38186-38189 IN denotes for
T8921 38190-38194 NN denotes Atrx
T8922 38195-38201 IN denotes during
T8923 38202-38210 JJ denotes neuronal
T8924 38211-38226 NN denotes differentiation
T8925 38227-38229 IN denotes in
T8926 38230-38235 JJ denotes adult
T8927 38236-38240 NNS denotes mice
T8928 38241-38242 -LRB- denotes [
T8929 38242-38244 CD denotes 20
T8930 38244-38245 -RRB- denotes ]
T8931 38245-38246 . denotes .
T8932 38246-38604 sentence denotes Further evidence that Atrx is also required at later stages of mouse development is provided by the observed dramatic skewing against Atrx-negative cells in some somatic tissues of carrier female mice, whose tissues initially comprise a mosaic of Atrx-positive and Atrx-negative cells as a result of random X-inactivation (M. Muers, personal communication).
T8933 38247-38254 JJ denotes Further
T8934 38255-38263 NN denotes evidence
T8936 38264-38268 IN denotes that
T8938 38269-38273 NN denotes Atrx
T8939 38274-38276 VBZ denotes is
T8940 38277-38281 RB denotes also
T8937 38282-38290 VBN denotes required
T8941 38291-38293 IN denotes at
T8942 38294-38299 JJ denotes later
T8943 38300-38306 NNS denotes stages
T8944 38307-38309 IN denotes of
T8945 38310-38315 NN denotes mouse
T8946 38316-38327 NN denotes development
T8947 38328-38330 VBZ denotes is
T8935 38331-38339 VBN denotes provided
T8948 38340-38342 IN denotes by
T8949 38343-38346 DT denotes the
T8951 38347-38355 VBN denotes observed
T8952 38356-38364 JJ denotes dramatic
T8950 38365-38372 NN denotes skewing
T8953 38373-38380 IN denotes against
T8954 38381-38385 NN denotes Atrx
T8956 38385-38386 HYPH denotes -
T8957 38386-38394 JJ denotes negative
T8955 38395-38400 NNS denotes cells
T8958 38401-38403 IN denotes in
T8959 38404-38408 DT denotes some
T8961 38409-38416 JJ denotes somatic
T8960 38417-38424 NNS denotes tissues
T8962 38425-38427 IN denotes of
T8963 38428-38435 NN denotes carrier
T8965 38436-38442 JJ denotes female
T8964 38443-38447 NNS denotes mice
T8966 38447-38449 , denotes ,
T8967 38449-38454 WP$ denotes whose
T8968 38455-38462 NNS denotes tissues
T8970 38463-38472 RB denotes initially
T8969 38473-38481 VBP denotes comprise
T8971 38482-38483 DT denotes a
T8972 38484-38490 NN denotes mosaic
T8973 38491-38493 IN denotes of
T8974 38494-38498 NN denotes Atrx
T8976 38498-38499 HYPH denotes -
T8975 38499-38507 JJ denotes positive
T8978 38508-38511 CC denotes and
T8979 38512-38516 NN denotes Atrx
T8981 38516-38517 HYPH denotes -
T8980 38517-38525 JJ denotes negative
T8977 38526-38531 NNS denotes cells
T8982 38532-38534 IN denotes as
T8983 38535-38536 DT denotes a
T8984 38537-38543 NN denotes result
T8985 38544-38546 IN denotes of
T8986 38547-38553 JJ denotes random
T8988 38554-38555 NN denotes X
T8989 38555-38556 HYPH denotes -
T8987 38556-38568 NN denotes inactivation
T8990 38569-38570 -LRB- denotes (
T8991 38570-38572 NNP denotes M.
T8992 38573-38578 NNP denotes Muers
T8993 38578-38580 , denotes ,
T8994 38580-38588 JJ denotes personal
T8995 38589-38602 NN denotes communication
T8996 38602-38603 -RRB- denotes )
T8997 38603-38604 . denotes .
T8998 38604-38791 sentence denotes Atrx joins an expanding list of mouse genes for which targeted disruption results in peri-implantation lethality as a result of trophoblast or placental abnormalities (reviewed in [25]).
T8999 38605-38609 NN denotes Atrx
T9000 38610-38615 VBZ denotes joins
T9001 38616-38618 DT denotes an
T9003 38619-38628 VBG denotes expanding
T9002 38629-38633 NN denotes list
T9004 38634-38636 IN denotes of
T9005 38637-38642 NN denotes mouse
T9006 38643-38648 NNS denotes genes
T9007 38649-38652 IN denotes for
T9009 38653-38658 WDT denotes which
T9010 38659-38667 VBN denotes targeted
T9011 38668-38678 NN denotes disruption
T9008 38679-38686 VBZ denotes results
T9012 38687-38689 IN denotes in
T9013 38690-38707 JJ denotes peri-implantation
T9014 38708-38717 NN denotes lethality
T9015 38718-38720 IN denotes as
T9016 38721-38722 DT denotes a
T9017 38723-38729 NN denotes result
T9018 38730-38732 IN denotes of
T9019 38733-38744 NN denotes trophoblast
T9021 38745-38747 CC denotes or
T9022 38748-38757 JJ denotes placental
T9020 38758-38771 NNS denotes abnormalities
T9023 38772-38773 -LRB- denotes (
T9024 38773-38781 VBN denotes reviewed
T9025 38782-38784 IN denotes in
T9026 38785-38786 -LRB- denotes [
T9027 38786-38788 CD denotes 25
T9028 38788-38789 -RRB- denotes ]
T9029 38789-38790 -RRB- denotes )
T9030 38790-38791 . denotes .
T9031 38791-38901 sentence denotes Comparison with other phenotypes might provide some insight into the role of Atrx in trophoblast development.
T9032 38792-38802 NN denotes Comparison
T9034 38803-38807 IN denotes with
T9035 38808-38813 JJ denotes other
T9036 38814-38824 NNS denotes phenotypes
T9037 38825-38830 MD denotes might
T9033 38831-38838 VB denotes provide
T9038 38839-38843 DT denotes some
T9039 38844-38851 NN denotes insight
T9040 38852-38856 IN denotes into
T9041 38857-38860 DT denotes the
T9042 38861-38865 NN denotes role
T9043 38866-38868 IN denotes of
T9044 38869-38873 NN denotes Atrx
T9045 38874-38876 IN denotes in
T9046 38877-38888 NN denotes trophoblast
T9047 38889-38900 NN denotes development
T9048 38900-38901 . denotes .
T9049 38901-39117 sentence denotes Atrx-mutant embryos progress further than embryos nullizygous for factors involved in the initial specification of trophoblast stem cells (such as Cdx2) or in stem cell maintenance and proliferation (such as Eomes).
T9050 38902-38906 NN denotes Atrx
T9052 38906-38907 HYPH denotes -
T9051 38907-38913 NN denotes mutant
T9053 38914-38921 NNS denotes embryos
T9054 38922-38930 VBP denotes progress
T9055 38931-38938 RB denotes further
T9056 38939-38943 IN denotes than
T9057 38944-38951 NNS denotes embryos
T9058 38952-38963 JJ denotes nullizygous
T9059 38964-38967 IN denotes for
T9060 38968-38975 NNS denotes factors
T9061 38976-38984 VBN denotes involved
T9062 38985-38987 IN denotes in
T9063 38988-38991 DT denotes the
T9065 38992-38999 JJ denotes initial
T9064 39000-39013 NN denotes specification
T9066 39014-39016 IN denotes of
T9067 39017-39028 NN denotes trophoblast
T9069 39029-39033 NN denotes stem
T9068 39034-39039 NNS denotes cells
T9070 39040-39041 -LRB- denotes (
T9072 39041-39045 JJ denotes such
T9071 39046-39048 IN denotes as
T9073 39049-39053 NN denotes Cdx2
T9074 39053-39054 -RRB- denotes )
T9075 39055-39057 CC denotes or
T9076 39058-39060 IN denotes in
T9077 39061-39065 NN denotes stem
T9079 39066-39070 NN denotes cell
T9078 39071-39082 NN denotes maintenance
T9080 39083-39086 CC denotes and
T9081 39087-39100 NN denotes proliferation
T9082 39101-39102 -LRB- denotes (
T9084 39102-39106 JJ denotes such
T9083 39107-39109 IN denotes as
T9085 39110-39115 NN denotes Eomes
T9086 39115-39116 -RRB- denotes )
T9087 39116-39117 . denotes .
T9088 39117-39353 sentence denotes Cdx2-mutant embryos fail to implant and die between 3.5 and 5.5 dpc [26], while Eomes-mutant blastocysts implant into the uterus, but arrest soon after implantation without forming organised embryonic or extraembryonic structures [27].
T9089 39118-39122 NN denotes Cdx2
T9091 39122-39123 HYPH denotes -
T9090 39123-39129 NN denotes mutant
T9092 39130-39137 NNS denotes embryos
T9093 39138-39142 VBP denotes fail
T9094 39143-39145 TO denotes to
T9095 39146-39153 VB denotes implant
T9096 39154-39157 CC denotes and
T9097 39158-39161 VBP denotes die
T9098 39162-39169 IN denotes between
T9099 39170-39173 CD denotes 3.5
T9101 39174-39177 CC denotes and
T9102 39178-39181 CD denotes 5.5
T9100 39182-39185 NN denotes dpc
T9103 39186-39187 -LRB- denotes [
T9104 39187-39189 CD denotes 26
T9105 39189-39190 -RRB- denotes ]
T9106 39190-39192 , denotes ,
T9107 39192-39197 IN denotes while
T9109 39198-39203 NN denotes Eomes
T9111 39203-39204 HYPH denotes -
T9110 39204-39210 NN denotes mutant
T9112 39211-39222 NNS denotes blastocysts
T9108 39223-39230 VBP denotes implant
T9113 39231-39235 IN denotes into
T9114 39236-39239 DT denotes the
T9115 39240-39246 NN denotes uterus
T9116 39246-39248 , denotes ,
T9117 39248-39251 CC denotes but
T9118 39252-39258 VBP denotes arrest
T9119 39259-39263 RB denotes soon
T9120 39264-39269 IN denotes after
T9121 39270-39282 NN denotes implantation
T9122 39283-39290 IN denotes without
T9123 39291-39298 VBG denotes forming
T9124 39299-39308 VBN denotes organised
T9126 39309-39318 JJ denotes embryonic
T9127 39319-39321 CC denotes or
T9128 39322-39336 JJ denotes extraembryonic
T9125 39337-39347 NNS denotes structures
T9129 39348-39349 -LRB- denotes [
T9130 39349-39351 CD denotes 27
T9131 39351-39352 -RRB- denotes ]
T9132 39352-39353 . denotes .
T9133 39353-39464 sentence denotes In contrast, Atrx-mutant embryos implant successfully and establish organised embryonic structures by 7.5 dpc.
T9134 39354-39356 IN denotes In
T9136 39357-39365 NN denotes contrast
T9137 39365-39367 , denotes ,
T9138 39367-39371 NN denotes Atrx
T9140 39371-39372 HYPH denotes -
T9139 39372-39378 NN denotes mutant
T9141 39379-39386 NNS denotes embryos
T9135 39387-39394 VBP denotes implant
T9142 39395-39407 RB denotes successfully
T9143 39408-39411 CC denotes and
T9144 39412-39421 VBP denotes establish
T9145 39422-39431 VBN denotes organised
T9147 39432-39441 JJ denotes embryonic
T9146 39442-39452 NNS denotes structures
T9148 39453-39455 IN denotes by
T9149 39456-39459 CD denotes 7.5
T9150 39460-39463 NN denotes dpc
T9151 39463-39464 . denotes .
T9152 39464-39601 sentence denotes The Atrx-mutant phenotype closely resembles that observed in mice nullizygous for the basic helix-loop-helix transcription factor Hand1.
T9153 39465-39468 DT denotes The
T9155 39469-39473 NN denotes Atrx
T9157 39473-39474 HYPH denotes -
T9156 39474-39480 NN denotes mutant
T9154 39481-39490 NN denotes phenotype
T9159 39491-39498 RB denotes closely
T9158 39499-39508 VBZ denotes resembles
T9160 39509-39513 DT denotes that
T9161 39514-39522 VBN denotes observed
T9162 39523-39525 IN denotes in
T9163 39526-39530 NNS denotes mice
T9164 39531-39542 JJ denotes nullizygous
T9165 39543-39546 IN denotes for
T9166 39547-39550 DT denotes the
T9168 39551-39556 JJ denotes basic
T9169 39557-39562 NN denotes helix
T9171 39562-39563 HYPH denotes -
T9172 39563-39567 NN denotes loop
T9173 39567-39568 HYPH denotes -
T9170 39568-39573 NN denotes helix
T9174 39574-39587 NN denotes transcription
T9167 39588-39594 NN denotes factor
T9175 39595-39600 NN denotes Hand1
T9176 39600-39601 . denotes .
T9177 39601-39827 sentence denotes Hand1-mutant conceptuses arrest at around 7.5 dpc and display a normal embryonic compartment, but, like Atrx-mutant embryos, ablation of Hand1 causes a reduction in the size of the ectoplacental cone and density of TGCs [28].
T9178 39602-39607 NN denotes Hand1
T9180 39607-39608 HYPH denotes -
T9179 39608-39614 NN denotes mutant
T9181 39615-39626 NNS denotes conceptuses
T9182 39627-39633 VBP denotes arrest
T9183 39634-39636 IN denotes at
T9184 39637-39643 IN denotes around
T9185 39644-39647 CD denotes 7.5
T9186 39648-39651 NN denotes dpc
T9187 39652-39655 CC denotes and
T9188 39656-39663 VBP denotes display
T9189 39664-39665 DT denotes a
T9191 39666-39672 JJ denotes normal
T9192 39673-39682 JJ denotes embryonic
T9190 39683-39694 NN denotes compartment
T9193 39694-39696 , denotes ,
T9194 39696-39699 CC denotes but
T9195 39699-39701 , denotes ,
T9196 39701-39705 IN denotes like
T9198 39706-39710 NN denotes Atrx
T9200 39710-39711 HYPH denotes -
T9199 39711-39717 NN denotes mutant
T9201 39718-39725 NNS denotes embryos
T9202 39725-39727 , denotes ,
T9203 39727-39735 NN denotes ablation
T9204 39736-39738 IN denotes of
T9205 39739-39744 NN denotes Hand1
T9197 39745-39751 VBZ denotes causes
T9206 39752-39753 DT denotes a
T9207 39754-39763 NN denotes reduction
T9208 39764-39766 IN denotes in
T9209 39767-39770 DT denotes the
T9210 39771-39775 NN denotes size
T9211 39776-39778 IN denotes of
T9212 39779-39782 DT denotes the
T9214 39783-39796 JJ denotes ectoplacental
T9213 39797-39801 NN denotes cone
T9215 39802-39805 CC denotes and
T9216 39806-39813 NN denotes density
T9217 39814-39816 IN denotes of
T9218 39817-39821 NNS denotes TGCs
T9219 39822-39823 -LRB- denotes [
T9220 39823-39825 CD denotes 28
T9221 39825-39826 -RRB- denotes ]
T9222 39826-39827 . denotes .
T9223 39827-39930 sentence denotes As with Atrx mutants, only arrested or resorbed Hand1-mutant conceptuses were recovered after 8.5 dpc.
T9224 39828-39830 IN denotes As
T9226 39831-39835 IN denotes with
T9227 39836-39840 NN denotes Atrx
T9228 39841-39848 NNS denotes mutants
T9229 39848-39850 , denotes ,
T9230 39850-39854 RB denotes only
T9232 39855-39863 VBN denotes arrested
T9233 39864-39866 CC denotes or
T9234 39867-39875 VBN denotes resorbed
T9235 39876-39881 NN denotes Hand1
T9237 39881-39882 HYPH denotes -
T9236 39882-39888 NN denotes mutant
T9231 39889-39900 NNS denotes conceptuses
T9238 39901-39905 VBD denotes were
T9225 39906-39915 VBN denotes recovered
T9239 39916-39921 IN denotes after
T9240 39922-39925 CD denotes 8.5
T9241 39926-39929 NN denotes dpc
T9242 39929-39930 . denotes .
T9243 39930-40088 sentence denotes Also like Atrx, disruption of Hand1 specifically affects secondary giant cell formation, and primary trophoblast outgrowths from blastocysts appeared normal.
T9244 39931-39935 RB denotes Also
T9246 39936-39940 IN denotes like
T9247 39941-39945 NN denotes Atrx
T9248 39945-39947 , denotes ,
T9249 39947-39957 NN denotes disruption
T9250 39958-39960 IN denotes of
T9251 39961-39966 NN denotes Hand1
T9252 39967-39979 RB denotes specifically
T9245 39980-39987 VBZ denotes affects
T9253 39988-39997 JJ denotes secondary
T9255 39998-40003 JJ denotes giant
T9254 40004-40008 NN denotes cell
T9256 40009-40018 NN denotes formation
T9257 40018-40020 , denotes ,
T9258 40020-40023 CC denotes and
T9259 40024-40031 JJ denotes primary
T9261 40032-40043 NN denotes trophoblast
T9260 40044-40054 NNS denotes outgrowths
T9263 40055-40059 IN denotes from
T9264 40060-40071 NNS denotes blastocysts
T9262 40072-40080 VBD denotes appeared
T9265 40081-40087 JJ denotes normal
T9266 40087-40088 . denotes .
T9267 40088-40254 sentence denotes Hand1 is required for terminal differentiation of secondary TGCs, and in its absence trophoblast cells arrest at a precursor stage in the ectoplacental cone [17,28].
T9268 40089-40094 NN denotes Hand1
T9270 40095-40097 VBZ denotes is
T9269 40098-40106 VBN denotes required
T9271 40107-40110 IN denotes for
T9272 40111-40119 JJ denotes terminal
T9273 40120-40135 NN denotes differentiation
T9274 40136-40138 IN denotes of
T9275 40139-40148 JJ denotes secondary
T9276 40149-40153 NNS denotes TGCs
T9277 40153-40155 , denotes ,
T9278 40155-40158 CC denotes and
T9279 40159-40161 IN denotes in
T9281 40162-40165 PRP$ denotes its
T9282 40166-40173 NN denotes absence
T9283 40174-40185 NN denotes trophoblast
T9284 40186-40191 NNS denotes cells
T9280 40192-40198 VBP denotes arrest
T9285 40199-40201 IN denotes at
T9286 40202-40203 DT denotes a
T9288 40204-40213 NN denotes precursor
T9287 40214-40219 NN denotes stage
T9289 40220-40222 IN denotes in
T9290 40223-40226 DT denotes the
T9292 40227-40240 JJ denotes ectoplacental
T9291 40241-40245 NN denotes cone
T9293 40246-40247 -LRB- denotes [
T9295 40247-40249 CD denotes 17
T9296 40249-40250 , denotes ,
T9294 40250-40252 CD denotes 28
T9297 40252-40253 -RRB- denotes ]
T9298 40253-40254 . denotes .
T9299 40254-40625 sentence denotes Given the similarity of the Atrx- and Hand1-mutant phenotypes and the likelihood that Atrx acts as a transcriptional regulator by modifying chromatin structure, it will be of interest to determine whether Atrx is itself a regulator of Hand1 expression, or alternatively whether it acts as a co-regulator of one or more of the downstream transcriptional targets of Hand1.
T9300 40255-40260 VBN denotes Given
T9302 40261-40264 DT denotes the
T9303 40265-40275 NN denotes similarity
T9304 40276-40278 IN denotes of
T9305 40279-40282 DT denotes the
T9307 40283-40287 NN denotes Atrx
T9309 40287-40288 HYPH denotes -
T9310 40289-40292 CC denotes and
T9311 40293-40298 NN denotes Hand1
T9312 40298-40299 HYPH denotes -
T9308 40299-40305 NN denotes mutant
T9306 40306-40316 NNS denotes phenotypes
T9313 40317-40320 CC denotes and
T9314 40321-40324 DT denotes the
T9315 40325-40335 NN denotes likelihood
T9316 40336-40340 IN denotes that
T9318 40341-40345 NN denotes Atrx
T9317 40346-40350 VBZ denotes acts
T9319 40351-40353 IN denotes as
T9320 40354-40355 DT denotes a
T9322 40356-40371 JJ denotes transcriptional
T9321 40372-40381 NN denotes regulator
T9323 40382-40384 IN denotes by
T9324 40385-40394 VBG denotes modifying
T9325 40395-40404 NN denotes chromatin
T9326 40405-40414 NN denotes structure
T9327 40414-40416 , denotes ,
T9328 40416-40418 PRP denotes it
T9329 40419-40423 MD denotes will
T9301 40424-40426 VB denotes be
T9330 40427-40429 IN denotes of
T9331 40430-40438 NN denotes interest
T9332 40439-40441 TO denotes to
T9333 40442-40451 VB denotes determine
T9334 40452-40459 IN denotes whether
T9336 40460-40464 NN denotes Atrx
T9335 40465-40467 VBZ denotes is
T9337 40468-40474 PRP denotes itself
T9338 40475-40476 DT denotes a
T9339 40477-40486 NN denotes regulator
T9340 40487-40489 IN denotes of
T9341 40490-40495 NN denotes Hand1
T9342 40496-40506 NN denotes expression
T9343 40506-40508 , denotes ,
T9344 40508-40510 CC denotes or
T9345 40511-40524 RB denotes alternatively
T9347 40525-40532 IN denotes whether
T9348 40533-40535 PRP denotes it
T9346 40536-40540 VBZ denotes acts
T9349 40541-40543 IN denotes as
T9350 40544-40545 DT denotes a
T9351 40546-40558 NN denotes co-regulator
T9352 40559-40561 IN denotes of
T9353 40562-40565 CD denotes one
T9354 40566-40568 CC denotes or
T9355 40569-40573 JJR denotes more
T9356 40574-40576 IN denotes of
T9357 40577-40580 DT denotes the
T9359 40581-40591 JJ denotes downstream
T9360 40592-40607 JJ denotes transcriptional
T9358 40608-40615 NNS denotes targets
T9361 40616-40618 IN denotes of
T9362 40619-40624 NN denotes Hand1
T9363 40624-40625 . denotes .
T9364 40625-40758 sentence denotes It is noteworthy that, in the brain-specific Atrx knockout mice, the defect was observed in terminally differentiating neurons [20].
T9365 40626-40628 PRP denotes It
T9366 40629-40631 VBZ denotes is
T9367 40632-40642 JJ denotes noteworthy
T9368 40643-40647 IN denotes that
T9370 40647-40649 , denotes ,
T9371 40649-40651 IN denotes in
T9372 40652-40655 DT denotes the
T9374 40656-40661 NN denotes brain
T9376 40661-40662 HYPH denotes -
T9375 40662-40670 JJ denotes specific
T9377 40671-40675 NN denotes Atrx
T9378 40676-40684 NN denotes knockout
T9373 40685-40689 NNS denotes mice
T9379 40689-40691 , denotes ,
T9380 40691-40694 DT denotes the
T9381 40695-40701 NN denotes defect
T9382 40702-40705 VBD denotes was
T9369 40706-40714 VBN denotes observed
T9383 40715-40717 IN denotes in
T9384 40718-40728 RB denotes terminally
T9385 40729-40744 VBG denotes differentiating
T9386 40745-40752 NNS denotes neurons
T9387 40753-40754 -LRB- denotes [
T9388 40754-40756 CD denotes 20
T9389 40756-40757 -RRB- denotes ]
T9390 40757-40758 . denotes .
T9391 40758-41067 sentence denotes The secondary TGCs affected in the universal Atrx knockout reported here represent one of the first terminally differentiated tissues in the developing mouse, and this may point to the requirement for Atrx in the high-level expression of some tissue-specific genes during the final stages of differentiation.
T9392 40759-40762 DT denotes The
T9394 40763-40772 JJ denotes secondary
T9393 40773-40777 NNS denotes TGCs
T9396 40778-40786 VBN denotes affected
T9397 40787-40789 IN denotes in
T9398 40790-40793 DT denotes the
T9400 40794-40803 JJ denotes universal
T9401 40804-40808 NN denotes Atrx
T9399 40809-40817 NN denotes knockout
T9402 40818-40826 VBN denotes reported
T9403 40827-40831 RB denotes here
T9395 40832-40841 VBP denotes represent
T9404 40842-40845 CD denotes one
T9405 40846-40848 IN denotes of
T9406 40849-40852 DT denotes the
T9408 40853-40858 JJ denotes first
T9409 40859-40869 RB denotes terminally
T9410 40870-40884 VBN denotes differentiated
T9407 40885-40892 NNS denotes tissues
T9411 40893-40895 IN denotes in
T9412 40896-40899 DT denotes the
T9414 40900-40910 VBG denotes developing
T9413 40911-40916 NN denotes mouse
T9415 40916-40918 , denotes ,
T9416 40918-40921 CC denotes and
T9417 40922-40926 DT denotes this
T9419 40927-40930 MD denotes may
T9418 40931-40936 VB denotes point
T9420 40937-40939 IN denotes to
T9421 40940-40943 DT denotes the
T9422 40944-40955 NN denotes requirement
T9423 40956-40959 IN denotes for
T9424 40960-40964 NN denotes Atrx
T9425 40965-40967 IN denotes in
T9426 40968-40971 DT denotes the
T9428 40972-40976 JJ denotes high
T9430 40976-40977 HYPH denotes -
T9429 40977-40982 NN denotes level
T9427 40983-40993 NN denotes expression
T9431 40994-40996 IN denotes of
T9432 40997-41001 DT denotes some
T9434 41002-41008 NN denotes tissue
T9436 41008-41009 HYPH denotes -
T9435 41009-41017 JJ denotes specific
T9433 41018-41023 NNS denotes genes
T9437 41024-41030 IN denotes during
T9438 41031-41034 DT denotes the
T9440 41035-41040 JJ denotes final
T9439 41041-41047 NNS denotes stages
T9441 41048-41050 IN denotes of
T9442 41051-41066 NN denotes differentiation
T9443 41066-41067 . denotes .
T9444 41067-41275 sentence denotes Interestingly, the α-globin genes, the only confirmed transcriptional targets of regulation by human ATRX, are also highly expressed specifically during terminal differentiation within the erythroid lineage.
T9445 41068-41081 RB denotes Interestingly
T9447 41081-41083 , denotes ,
T9448 41083-41086 DT denotes the
T9450 41087-41088 NN denotes α
T9452 41088-41089 HYPH denotes -
T9451 41089-41095 NN denotes globin
T9449 41096-41101 NNS denotes genes
T9453 41101-41103 , denotes ,
T9454 41103-41106 DT denotes the
T9456 41107-41111 RB denotes only
T9457 41112-41121 VBN denotes confirmed
T9458 41122-41137 JJ denotes transcriptional
T9455 41138-41145 NNS denotes targets
T9459 41146-41148 IN denotes of
T9460 41149-41159 NN denotes regulation
T9461 41160-41162 IN denotes by
T9462 41163-41168 JJ denotes human
T9463 41169-41173 NN denotes ATRX
T9464 41173-41175 , denotes ,
T9465 41175-41178 VBP denotes are
T9466 41179-41183 RB denotes also
T9467 41184-41190 RB denotes highly
T9446 41191-41200 VBN denotes expressed
T9468 41201-41213 RB denotes specifically
T9469 41214-41220 IN denotes during
T9470 41221-41229 JJ denotes terminal
T9471 41230-41245 NN denotes differentiation
T9472 41246-41252 IN denotes within
T9473 41253-41256 DT denotes the
T9475 41257-41266 JJ denotes erythroid
T9474 41267-41274 NN denotes lineage
T9476 41274-41275 . denotes .
T9932 41277-41281 NN denotes Atrx
T9933 41282-41289 VBZ denotes Escapes
T9934 41290-41299 VBN denotes Imprinted
T9936 41300-41301 NN denotes X
T9937 41301-41302 HYPH denotes -
T9935 41302-41314 NN denotes Inactivation
T9938 41315-41317 IN denotes in
T9939 41318-41332 JJ denotes Extraembryonic
T9940 41333-41340 NNS denotes Tissues
T9941 41341-41343 IN denotes of
T9942 41344-41351 NN denotes Carrier
T9944 41352-41358 JJ denotes Female
T9943 41359-41363 NNS denotes Mice
T9945 41363-41646 sentence denotes Another surprising finding of this study is that, in carrier female embryos, a paternally inherited Atrx WT allele appears to escape the process of imprinted X-inactivation, which ordinarily silences the Xp chromosome in the extraembryonic compartment of female murine tissues [18].
T9946 41364-41371 DT denotes Another
T9948 41372-41382 JJ denotes surprising
T9947 41383-41390 NN denotes finding
T9950 41391-41393 IN denotes of
T9951 41394-41398 DT denotes this
T9952 41399-41404 NN denotes study
T9949 41405-41407 VBZ denotes is
T9953 41408-41412 IN denotes that
T9955 41412-41414 , denotes ,
T9956 41414-41416 IN denotes in
T9957 41417-41424 NN denotes carrier
T9959 41425-41431 JJ denotes female
T9958 41432-41439 NNS denotes embryos
T9960 41439-41441 , denotes ,
T9961 41441-41442 DT denotes a
T9963 41443-41453 RB denotes paternally
T9964 41454-41463 VBN denotes inherited
T9965 41464-41471 NN denotes Atrx WT
T9962 41472-41478 NN denotes allele
T9954 41479-41486 VBZ denotes appears
T9966 41487-41489 TO denotes to
T9967 41490-41496 VB denotes escape
T9968 41497-41500 DT denotes the
T9969 41501-41508 NN denotes process
T9970 41509-41511 IN denotes of
T9971 41512-41521 VBN denotes imprinted
T9973 41522-41523 NN denotes X
T9974 41523-41524 HYPH denotes -
T9972 41524-41536 NN denotes inactivation
T9975 41536-41538 , denotes ,
T9976 41538-41543 WDT denotes which
T9978 41544-41554 RB denotes ordinarily
T9977 41555-41563 VBZ denotes silences
T9979 41564-41567 DT denotes the
T9981 41568-41570 NN denotes Xp
T9980 41571-41581 NN denotes chromosome
T9982 41582-41584 IN denotes in
T9983 41585-41588 DT denotes the
T9985 41589-41603 JJ denotes extraembryonic
T9984 41604-41615 NN denotes compartment
T9986 41616-41618 IN denotes of
T9987 41619-41625 JJ denotes female
T9989 41626-41632 JJ denotes murine
T9988 41633-41640 NNS denotes tissues
T9990 41641-41642 -LRB- denotes [
T9991 41642-41644 CD denotes 18
T9992 41644-41645 -RRB- denotes ]
T9993 41645-41646 . denotes .
T9994 41646-41830 sentence denotes Silencing of the Atrx WT allele on Xp should render these females null for Atrx in the extraembryonic tissues, since the normally active Xm chromosome carries the Atrx Δ18Δneo allele.
T9995 41647-41656 NN denotes Silencing
T9997 41657-41659 IN denotes of
T9998 41660-41663 DT denotes the
T10000 41664-41671 NN denotes Atrx WT
T9999 41672-41678 NN denotes allele
T10001 41679-41681 IN denotes on
T10002 41682-41684 NN denotes Xp
T10003 41685-41691 MD denotes should
T9996 41692-41698 VB denotes render
T10004 41699-41704 DT denotes these
T10005 41705-41712 NNS denotes females
T10006 41713-41717 JJ denotes null
T10007 41718-41721 IN denotes for
T10008 41722-41726 NN denotes Atrx
T10009 41727-41729 IN denotes in
T10010 41730-41733 DT denotes the
T10012 41734-41748 JJ denotes extraembryonic
T10011 41749-41756 NNS denotes tissues
T10013 41756-41758 , denotes ,
T10014 41758-41763 IN denotes since
T10016 41764-41767 DT denotes the
T10018 41768-41776 RB denotes normally
T10019 41777-41783 JJ denotes active
T10020 41784-41786 NN denotes Xm
T10017 41787-41797 NN denotes chromosome
T10015 41798-41805 VBZ denotes carries
T10021 41806-41809 DT denotes the
T10023 41810-41822 NN denotes Atrx Δ18Δneo
T10022 41823-41829 NN denotes allele
T10024 41829-41830 . denotes .
T10025 41830-41936 sentence denotes Although not phenotypically normal, some Atrx carrier females developed to term and went on to reproduce.
T10026 41831-41839 IN denotes Although
T10028 41840-41843 RB denotes not
T10029 41844-41858 RB denotes phenotypically
T10027 41859-41865 JJ denotes normal
T10031 41865-41867 , denotes ,
T10032 41867-41871 DT denotes some
T10034 41872-41876 NN denotes Atrx
T10035 41877-41884 NN denotes carrier
T10033 41885-41892 NNS denotes females
T10030 41893-41902 VBD denotes developed
T10036 41903-41905 IN denotes to
T10037 41906-41910 NN denotes term
T10038 41911-41914 CC denotes and
T10039 41915-41919 VBD denotes went
T10040 41920-41922 RP denotes on
T10041 41923-41925 TO denotes to
T10042 41926-41935 VB denotes reproduce
T10043 41935-41936 . denotes .
T10044 41936-42254 sentence denotes Thus, the failure to correctly silence the paternally derived Atrx WT allele in the extraembryonic tissues of carrier females is consistent with our observations that in Atrxnull males, the Atrx protein plays an essential role in the development of the trophoblast and is necessary for survival in utero in the mouse.
T10045 41937-41941 RB denotes Thus
T10047 41941-41943 , denotes ,
T10048 41943-41946 DT denotes the
T10049 41947-41954 NN denotes failure
T10050 41955-41957 TO denotes to
T10052 41958-41967 RB denotes correctly
T10051 41968-41975 VB denotes silence
T10053 41976-41979 DT denotes the
T10055 41980-41990 RB denotes paternally
T10056 41991-41998 VBN denotes derived
T10057 41999-42006 NN denotes Atrx WT
T10054 42007-42013 NN denotes allele
T10058 42014-42016 IN denotes in
T10059 42017-42020 DT denotes the
T10061 42021-42035 JJ denotes extraembryonic
T10060 42036-42043 NNS denotes tissues
T10062 42044-42046 IN denotes of
T10063 42047-42054 NN denotes carrier
T10064 42055-42062 NNS denotes females
T10046 42063-42065 VBZ denotes is
T10065 42066-42076 JJ denotes consistent
T10066 42077-42081 IN denotes with
T10067 42082-42085 PRP$ denotes our
T10068 42086-42098 NNS denotes observations
T10069 42099-42103 IN denotes that
T10071 42104-42106 IN denotes in
T10072 42107-42115 JJ denotes Atrxnull
T10073 42116-42121 NNS denotes males
T10074 42121-42123 , denotes ,
T10075 42123-42126 DT denotes the
T10077 42127-42131 NN denotes Atrx
T10076 42132-42139 NN denotes protein
T10070 42140-42145 VBZ denotes plays
T10078 42146-42148 DT denotes an
T10080 42149-42158 JJ denotes essential
T10079 42159-42163 NN denotes role
T10081 42164-42166 IN denotes in
T10082 42167-42170 DT denotes the
T10083 42171-42182 NN denotes development
T10084 42183-42185 IN denotes of
T10085 42186-42189 DT denotes the
T10086 42190-42201 NN denotes trophoblast
T10087 42202-42205 CC denotes and
T10088 42206-42208 VBZ denotes is
T10089 42209-42218 JJ denotes necessary
T10090 42219-42222 IN denotes for
T10091 42223-42231 NN denotes survival
T10092 42232-42234 FW denotes in
T10093 42235-42240 FW denotes utero
T10094 42241-42243 IN denotes in
T10095 42244-42247 DT denotes the
T10096 42248-42253 NN denotes mouse
T10097 42253-42254 . denotes .
T10098 42254-42439 sentence denotes The survival of Atrx carrier females contrasts with the phenotypes seen in carriers of mutations of other murine X-linked genes known to be essential in the extraembryonic compartment.
T10099 42255-42258 DT denotes The
T10100 42259-42267 NN denotes survival
T10102 42268-42270 IN denotes of
T10103 42271-42275 NN denotes Atrx
T10105 42276-42283 NN denotes carrier
T10104 42284-42291 NNS denotes females
T10101 42292-42301 VBZ denotes contrasts
T10106 42302-42306 IN denotes with
T10107 42307-42310 DT denotes the
T10108 42311-42321 NNS denotes phenotypes
T10109 42322-42326 VBN denotes seen
T10110 42327-42329 IN denotes in
T10111 42330-42338 NNS denotes carriers
T10112 42339-42341 IN denotes of
T10113 42342-42351 NNS denotes mutations
T10114 42352-42354 IN denotes of
T10115 42355-42360 JJ denotes other
T10117 42361-42367 JJ denotes murine
T10118 42368-42369 NN denotes X
T10120 42369-42370 HYPH denotes -
T10119 42370-42376 VBN denotes linked
T10116 42377-42382 NNS denotes genes
T10121 42383-42388 VBN denotes known
T10122 42389-42391 TO denotes to
T10123 42392-42394 VB denotes be
T10124 42395-42404 JJ denotes essential
T10125 42405-42407 IN denotes in
T10126 42408-42411 DT denotes the
T10128 42412-42426 JJ denotes extraembryonic
T10127 42427-42438 NN denotes compartment
T10129 42438-42439 . denotes .
T10130 42439-42678 sentence denotes For example, targeted disruption of the dyskerin (Dkc1), glucose 6-phosphate dehydrogenase (G6PD), and choroideremia (Chm) genes cause embryonic lethality in null male embryos through defects of the extraembryonic-derived tissues [29–31].
T10131 42440-42443 IN denotes For
T10133 42444-42451 NN denotes example
T10134 42451-42453 , denotes ,
T10135 42453-42461 VBN denotes targeted
T10136 42462-42472 NN denotes disruption
T10137 42473-42475 IN denotes of
T10138 42476-42479 DT denotes the
T10140 42480-42488 NN denotes dyskerin
T10141 42489-42490 -LRB- denotes (
T10142 42490-42494 NN denotes Dkc1
T10143 42494-42495 -RRB- denotes )
T10144 42495-42497 , denotes ,
T10145 42497-42504 NN denotes glucose
T10147 42505-42506 CD denotes 6
T10148 42506-42507 HYPH denotes -
T10146 42507-42516 NN denotes phosphate
T10149 42517-42530 NN denotes dehydrogenase
T10150 42531-42532 -LRB- denotes (
T10151 42532-42536 NN denotes G6PD
T10152 42536-42537 -RRB- denotes )
T10153 42537-42539 , denotes ,
T10154 42539-42542 CC denotes and
T10155 42543-42556 NN denotes choroideremia
T10156 42557-42558 -LRB- denotes (
T10157 42558-42561 NN denotes Chm
T10158 42561-42562 -RRB- denotes )
T10139 42563-42568 NNS denotes genes
T10132 42569-42574 VBP denotes cause
T10159 42575-42584 JJ denotes embryonic
T10160 42585-42594 NN denotes lethality
T10161 42595-42597 IN denotes in
T10162 42598-42602 JJ denotes null
T10164 42603-42607 JJ denotes male
T10163 42608-42615 NNS denotes embryos
T10165 42616-42623 IN denotes through
T10166 42624-42631 NNS denotes defects
T10167 42632-42634 IN denotes of
T10168 42635-42638 DT denotes the
T10170 42639-42653 JJ denotes extraembryonic
T10172 42653-42654 HYPH denotes -
T10171 42654-42661 VBN denotes derived
T10169 42662-42669 NNS denotes tissues
T10173 42670-42671 -LRB- denotes [
T10174 42671-42673 CD denotes 29
T10175 42673-42674 SYM denotes –
T10176 42674-42676 CD denotes 31
T10177 42676-42677 -RRB- denotes ]
T10178 42677-42678 . denotes .
T10179 42678-42856 sentence denotes Female mice carrying mutations of these genes on the maternally inherited X chromosome also die in utero, whereas females that inherit the mutation on the Xp chromosome survive.
T10180 42679-42685 JJ denotes Female
T10181 42686-42690 NNS denotes mice
T10183 42691-42699 VBG denotes carrying
T10184 42700-42709 NNS denotes mutations
T10185 42710-42712 IN denotes of
T10186 42713-42718 DT denotes these
T10187 42719-42724 NNS denotes genes
T10188 42725-42727 IN denotes on
T10189 42728-42731 DT denotes the
T10191 42732-42742 RB denotes maternally
T10192 42743-42752 VBN denotes inherited
T10193 42753-42754 NN denotes X
T10190 42755-42765 NN denotes chromosome
T10194 42766-42770 RB denotes also
T10182 42771-42774 VBP denotes die
T10195 42775-42777 FW denotes in
T10196 42778-42783 FW denotes utero
T10197 42783-42785 , denotes ,
T10198 42785-42792 IN denotes whereas
T10200 42793-42800 NNS denotes females
T10201 42801-42805 WDT denotes that
T10202 42806-42813 VBP denotes inherit
T10203 42814-42817 DT denotes the
T10204 42818-42826 NN denotes mutation
T10205 42827-42829 IN denotes on
T10206 42830-42833 DT denotes the
T10208 42834-42836 NN denotes Xp
T10207 42837-42847 NN denotes chromosome
T10199 42848-42855 VBP denotes survive
T10209 42855-42856 . denotes .
T10210 42856-43077 sentence denotes Thus, unlike Atrx, these genes and/or their effects on cell growth are unable to circumvent the processes that ultimately cause all cells in the extraembryonic tissues to express only the maternally derived X chromosome.
T10211 42857-42861 RB denotes Thus
T10213 42861-42863 , denotes ,
T10214 42863-42869 IN denotes unlike
T10215 42870-42874 NN denotes Atrx
T10216 42874-42876 , denotes ,
T10217 42876-42881 DT denotes these
T10218 42882-42887 NNS denotes genes
T10219 42888-42891 CC denotes and
T10220 42891-42892 HYPH denotes /
T10221 42892-42894 CC denotes or
T10222 42895-42900 PRP$ denotes their
T10223 42901-42908 NNS denotes effects
T10224 42909-42911 IN denotes on
T10225 42912-42916 NN denotes cell
T10226 42917-42923 NN denotes growth
T10212 42924-42927 VBP denotes are
T10227 42928-42934 JJ denotes unable
T10228 42935-42937 TO denotes to
T10229 42938-42948 VB denotes circumvent
T10230 42949-42952 DT denotes the
T10231 42953-42962 NNS denotes processes
T10232 42963-42967 WDT denotes that
T10234 42968-42978 RB denotes ultimately
T10233 42979-42984 VBP denotes cause
T10235 42985-42988 DT denotes all
T10236 42989-42994 NNS denotes cells
T10238 42995-42997 IN denotes in
T10239 42998-43001 DT denotes the
T10241 43002-43016 JJ denotes extraembryonic
T10240 43017-43024 NNS denotes tissues
T10242 43025-43027 TO denotes to
T10237 43028-43035 VB denotes express
T10243 43036-43040 RB denotes only
T10245 43041-43044 DT denotes the
T10246 43045-43055 RB denotes maternally
T10247 43056-43063 VBN denotes derived
T10248 43064-43065 NN denotes X
T10244 43066-43076 NN denotes chromosome
T10249 43076-43077 . denotes .
T10250 43077-43202 sentence denotes How might expression of the paternal Atrx WT allele be maintained in the extraembryonic tissues of the Atrx carrier females?
T10251 43078-43081 WRB denotes How
T10253 43082-43087 MD denotes might
T10254 43088-43098 NN denotes expression
T10255 43099-43101 IN denotes of
T10256 43102-43105 DT denotes the
T10258 43106-43114 JJ denotes paternal
T10259 43115-43122 NN denotes Atrx WT
T10257 43123-43129 NN denotes allele
T10260 43130-43132 VB denotes be
T10252 43133-43143 VBN denotes maintained
T10261 43144-43146 IN denotes in
T10262 43147-43150 DT denotes the
T10264 43151-43165 JJ denotes extraembryonic
T10263 43166-43173 NNS denotes tissues
T10265 43174-43176 IN denotes of
T10266 43177-43180 DT denotes the
T10268 43181-43185 NN denotes Atrx
T10269 43186-43193 NN denotes carrier
T10267 43194-43201 NNS denotes females
T10270 43201-43202 . denotes ?
T10271 43202-43436 sentence denotes One possibility is that, like some other X-linked genes, silencing of the Atrx gene on Xp is incomplete, such that there is always a low-level, leaky output of Atrx from a normally inactivated Xp chromosome in extraembryonic tissues.
T10272 43203-43206 CD denotes One
T10273 43207-43218 NN denotes possibility
T10274 43219-43221 VBZ denotes is
T10275 43222-43226 IN denotes that
T10277 43226-43228 , denotes ,
T10278 43228-43232 IN denotes like
T10279 43233-43237 DT denotes some
T10281 43238-43243 JJ denotes other
T10282 43244-43245 NN denotes X
T10284 43245-43246 HYPH denotes -
T10283 43246-43252 JJ denotes linked
T10280 43253-43258 NNS denotes genes
T10285 43258-43260 , denotes ,
T10286 43260-43269 NN denotes silencing
T10287 43270-43272 IN denotes of
T10288 43273-43276 DT denotes the
T10290 43277-43281 NN denotes Atrx
T10289 43282-43286 NN denotes gene
T10291 43287-43289 IN denotes on
T10292 43290-43292 NN denotes Xp
T10276 43293-43295 VBZ denotes is
T10293 43296-43306 JJ denotes incomplete
T10294 43306-43308 , denotes ,
T10295 43308-43312 JJ denotes such
T10297 43313-43317 IN denotes that
T10298 43318-43323 EX denotes there
T10296 43324-43326 VBZ denotes is
T10299 43327-43333 RB denotes always
T10300 43334-43335 DT denotes a
T10302 43336-43339 JJ denotes low
T10304 43339-43340 HYPH denotes -
T10303 43340-43345 NN denotes level
T10305 43345-43347 , denotes ,
T10306 43347-43352 JJ denotes leaky
T10301 43353-43359 NN denotes output
T10307 43360-43362 IN denotes of
T10308 43363-43367 NN denotes Atrx
T10309 43368-43372 IN denotes from
T10310 43373-43374 DT denotes a
T10312 43375-43383 RB denotes normally
T10313 43384-43395 VBN denotes inactivated
T10314 43396-43398 NN denotes Xp
T10311 43399-43409 NN denotes chromosome
T10315 43410-43412 IN denotes in
T10316 43413-43427 JJ denotes extraembryonic
T10317 43428-43435 NNS denotes tissues
T10318 43435-43436 . denotes .
T10319 43436-43714 sentence denotes However, it was recently demonstrated that the paternal Atrx (called Xnp) allele is completely silenced in a normal mouse trophoblast stem cell line [32], suggesting that Atrx does not normally escape imprinted X-inactivation in the extraembryonic tissues of wild-type females.
T10320 43437-43444 RB denotes However
T10322 43444-43446 , denotes ,
T10323 43446-43448 PRP denotes it
T10324 43449-43452 VBD denotes was
T10325 43453-43461 RB denotes recently
T10321 43462-43474 VBN denotes demonstrated
T10326 43475-43479 IN denotes that
T10328 43480-43483 DT denotes the
T10330 43484-43492 JJ denotes paternal
T10331 43493-43497 NN denotes Atrx
T10332 43498-43499 -LRB- denotes (
T10333 43499-43505 VBN denotes called
T10334 43506-43509 NN denotes Xnp
T10335 43509-43510 -RRB- denotes )
T10329 43511-43517 NN denotes allele
T10336 43518-43520 VBZ denotes is
T10337 43521-43531 RB denotes completely
T10327 43532-43540 VBN denotes silenced
T10338 43541-43543 IN denotes in
T10339 43544-43545 DT denotes a
T10341 43546-43552 JJ denotes normal
T10342 43553-43558 NN denotes mouse
T10343 43559-43570 NN denotes trophoblast
T10344 43571-43575 NN denotes stem
T10345 43576-43580 NN denotes cell
T10340 43581-43585 NN denotes line
T10346 43586-43587 -LRB- denotes [
T10347 43587-43589 CD denotes 32
T10348 43589-43590 -RRB- denotes ]
T10349 43590-43592 , denotes ,
T10350 43592-43602 VBG denotes suggesting
T10351 43603-43607 IN denotes that
T10353 43608-43612 NN denotes Atrx
T10354 43613-43617 VBZ denotes does
T10355 43618-43621 RB denotes not
T10356 43622-43630 RB denotes normally
T10352 43631-43637 VB denotes escape
T10357 43638-43647 VBN denotes imprinted
T10359 43648-43649 NN denotes X
T10360 43649-43650 HYPH denotes -
T10358 43650-43662 NN denotes inactivation
T10361 43663-43665 IN denotes in
T10362 43666-43669 DT denotes the
T10364 43670-43684 JJ denotes extraembryonic
T10363 43685-43692 NNS denotes tissues
T10365 43693-43695 IN denotes of
T10366 43696-43700 JJ denotes wild
T10368 43700-43701 HYPH denotes -
T10367 43701-43705 NN denotes type
T10369 43706-43713 NNS denotes females
T10370 43713-43714 . denotes .
T10371 43714-43838 sentence denotes Thus, the expression of the Xp-linked Atrx WT allele that we observed is unique to female carriers of the Atrx null allele.
T10372 43715-43719 RB denotes Thus
T10374 43719-43721 , denotes ,
T10375 43721-43724 DT denotes the
T10376 43725-43735 NN denotes expression
T10377 43736-43738 IN denotes of
T10378 43739-43742 DT denotes the
T10380 43743-43745 NN denotes Xp
T10382 43745-43746 HYPH denotes -
T10381 43746-43752 VBN denotes linked
T10383 43753-43760 NN denotes Atrx WT
T10379 43761-43767 NN denotes allele
T10384 43768-43772 WDT denotes that
T10386 43773-43775 PRP denotes we
T10385 43776-43784 VBD denotes observed
T10373 43785-43787 VBZ denotes is
T10387 43788-43794 JJ denotes unique
T10388 43795-43797 IN denotes to
T10389 43798-43804 JJ denotes female
T10390 43805-43813 NNS denotes carriers
T10391 43814-43816 IN denotes of
T10392 43817-43820 DT denotes the
T10394 43821-43830 JJ denotes Atrx null
T10393 43831-43837 NN denotes allele
T10395 43837-43838 . denotes .
T10396 43838-44159 sentence denotes Perhaps a more likely explanation for this phenomenon stems from experimental observations suggesting that imprinted X-inactivation is not imposed on all precursors of the mouse extraembryonic tissues: A subpopulation of cells may escape this process and make a random “choice” of which X chromosome will be inactivated.
T10397 43839-43846 RB denotes Perhaps
T10399 43847-43848 DT denotes a
T10401 43849-43853 RBR denotes more
T10402 43854-43860 JJ denotes likely
T10400 43861-43872 NN denotes explanation
T10403 43873-43876 IN denotes for
T10404 43877-43881 DT denotes this
T10405 43882-43892 NN denotes phenomenon
T10398 43893-43898 VBZ denotes stems
T10406 43899-43903 IN denotes from
T10407 43904-43916 JJ denotes experimental
T10408 43917-43929 NNS denotes observations
T10409 43930-43940 VBG denotes suggesting
T10410 43941-43945 IN denotes that
T10412 43946-43955 VBN denotes imprinted
T10414 43956-43957 NN denotes X
T10415 43957-43958 HYPH denotes -
T10413 43958-43970 NN denotes inactivation
T10417 43971-43973 VBZ denotes is
T10418 43974-43977 RB denotes not
T10416 43978-43985 VBN denotes imposed
T10419 43986-43988 IN denotes on
T10420 43989-43992 DT denotes all
T10421 43993-44003 NNS denotes precursors
T10422 44004-44006 IN denotes of
T10423 44007-44010 DT denotes the
T10425 44011-44016 NN denotes mouse
T10426 44017-44031 JJ denotes extraembryonic
T10424 44032-44039 NNS denotes tissues
T10427 44039-44041 : denotes :
T10428 44041-44042 DT denotes A
T10429 44043-44056 NN denotes subpopulation
T10430 44057-44059 IN denotes of
T10431 44060-44065 NNS denotes cells
T10432 44066-44069 MD denotes may
T10411 44070-44076 VB denotes escape
T10433 44077-44081 DT denotes this
T10434 44082-44089 NN denotes process
T10435 44090-44093 CC denotes and
T10436 44094-44098 VB denotes make
T10437 44099-44100 DT denotes a
T10439 44101-44107 JJ denotes random
T10440 44108-44109 `` denotes “
T10438 44109-44115 NN denotes choice
T10441 44115-44116 '' denotes ”
T10442 44117-44119 IN denotes of
T10443 44120-44125 WDT denotes which
T10445 44126-44127 NN denotes X
T10444 44128-44138 NN denotes chromosome
T10447 44139-44143 MD denotes will
T10448 44144-44146 VB denotes be
T10446 44147-44158 VBN denotes inactivated
T10449 44158-44159 . denotes .
T10450 44159-44287 sentence denotes On average, 50% of the cells in this randomly inactivating subpopulation would be expected to maintain an active Xp chromosome.
T10451 44160-44162 IN denotes On
T10453 44163-44170 JJ denotes average
T10454 44170-44172 , denotes ,
T10455 44172-44174 CD denotes 50
T10456 44174-44175 NN denotes %
T10457 44176-44178 IN denotes of
T10458 44179-44182 DT denotes the
T10459 44183-44188 NNS denotes cells
T10460 44189-44191 IN denotes in
T10461 44192-44196 DT denotes this
T10463 44197-44205 RB denotes randomly
T10464 44206-44218 VBG denotes inactivating
T10462 44219-44232 NN denotes subpopulation
T10465 44233-44238 MD denotes would
T10466 44239-44241 VB denotes be
T10452 44242-44250 VBN denotes expected
T10467 44251-44253 TO denotes to
T10468 44254-44262 VB denotes maintain
T10469 44263-44265 DT denotes an
T10471 44266-44272 JJ denotes active
T10472 44273-44275 NN denotes Xp
T10470 44276-44286 NN denotes chromosome
T10473 44286-44287 . denotes .
T10474 44287-44503 sentence denotes In support of this hypothesis, it has been demonstrated that expression of paternally transmitted X-linked lacZ [33,34] and GFP [35] transgenes failed to be silenced in a small subpopulation of extraembryonic cells.
T10475 44288-44290 IN denotes In
T10477 44291-44298 NN denotes support
T10478 44299-44301 IN denotes of
T10479 44302-44306 DT denotes this
T10480 44307-44317 NN denotes hypothesis
T10481 44317-44319 , denotes ,
T10482 44319-44321 PRP denotes it
T10483 44322-44325 VBZ denotes has
T10484 44326-44330 VBN denotes been
T10476 44331-44343 VBN denotes demonstrated
T10485 44344-44348 IN denotes that
T10487 44349-44359 NN denotes expression
T10488 44360-44362 IN denotes of
T10489 44363-44373 RB denotes paternally
T10490 44374-44385 VBN denotes transmitted
T10492 44386-44387 NN denotes X
T10494 44387-44388 HYPH denotes -
T10493 44388-44394 VBN denotes linked
T10491 44395-44399 NN denotes lacZ
T10496 44400-44401 -LRB- denotes [
T10498 44401-44403 CD denotes 33
T10499 44403-44404 , denotes ,
T10497 44404-44406 CD denotes 34
T10500 44406-44407 -RRB- denotes ]
T10501 44408-44411 CC denotes and
T10502 44412-44415 NN denotes GFP
T10503 44416-44417 -LRB- denotes [
T10504 44417-44419 CD denotes 35
T10505 44419-44420 -RRB- denotes ]
T10495 44421-44431 NNS denotes transgenes
T10486 44432-44438 VBD denotes failed
T10506 44439-44441 TO denotes to
T10508 44442-44444 VB denotes be
T10507 44445-44453 VBN denotes silenced
T10509 44454-44456 IN denotes in
T10510 44457-44458 DT denotes a
T10512 44459-44464 JJ denotes small
T10511 44465-44478 NN denotes subpopulation
T10513 44479-44481 IN denotes of
T10514 44482-44496 JJ denotes extraembryonic
T10515 44497-44502 NNS denotes cells
T10516 44502-44503 . denotes .
T10517 44503-44697 sentence denotes Further, it has been shown that in a subpopulation of extraembryonic cells, it is the Xm rather than the Xp that undergoes late replication, a molecular correlate of the inactive state [18,36].
T10518 44504-44511 RB denotes Further
T10520 44511-44513 , denotes ,
T10521 44513-44515 PRP denotes it
T10522 44516-44519 VBZ denotes has
T10523 44520-44524 VBN denotes been
T10519 44525-44530 VBN denotes shown
T10524 44531-44535 IN denotes that
T10526 44536-44538 IN denotes in
T10527 44539-44540 DT denotes a
T10528 44541-44554 NN denotes subpopulation
T10529 44555-44557 IN denotes of
T10530 44558-44572 JJ denotes extraembryonic
T10531 44573-44578 NNS denotes cells
T10532 44578-44580 , denotes ,
T10533 44580-44582 PRP denotes it
T10525 44583-44585 VBZ denotes is
T10534 44586-44589 DT denotes the
T10535 44590-44592 NN denotes Xm
T10536 44593-44599 JJ denotes rather
T10537 44600-44604 IN denotes than
T10538 44605-44608 DT denotes the
T10539 44609-44611 NN denotes Xp
T10540 44612-44616 WDT denotes that
T10541 44617-44626 VBZ denotes undergoes
T10542 44627-44631 JJ denotes late
T10543 44632-44643 NN denotes replication
T10544 44643-44645 , denotes ,
T10545 44645-44646 DT denotes a
T10547 44647-44656 JJ denotes molecular
T10546 44657-44666 NN denotes correlate
T10548 44667-44669 IN denotes of
T10549 44670-44673 DT denotes the
T10551 44674-44682 JJ denotes inactive
T10550 44683-44688 NN denotes state
T10552 44689-44690 -LRB- denotes [
T10554 44690-44692 CD denotes 18
T10555 44692-44693 , denotes ,
T10553 44693-44695 CD denotes 36
T10556 44695-44696 -RRB- denotes ]
T10557 44696-44697 . denotes .
T10558 44697-44985 sentence denotes Although initially small and quickly diluted in normal embryos, the cellular subpopulation that inactivates the Xm chromosome could rapidly expand to replace the normally imprinted cells in extraembryonic lineages if the normal silencing of Xp compromises cell growth or differentiation.
T10559 44698-44706 IN denotes Although
T10561 44707-44716 RB denotes initially
T10560 44717-44722 JJ denotes small
T10563 44723-44726 CC denotes and
T10564 44727-44734 RB denotes quickly
T10565 44735-44742 VBN denotes diluted
T10566 44743-44745 IN denotes in
T10567 44746-44752 JJ denotes normal
T10568 44753-44760 NNS denotes embryos
T10569 44760-44762 , denotes ,
T10570 44762-44765 DT denotes the
T10572 44766-44774 JJ denotes cellular
T10571 44775-44788 NN denotes subpopulation
T10573 44789-44793 WDT denotes that
T10574 44794-44805 VBZ denotes inactivates
T10575 44806-44809 DT denotes the
T10577 44810-44812 NN denotes Xm
T10576 44813-44823 NN denotes chromosome
T10578 44824-44829 MD denotes could
T10579 44830-44837 RB denotes rapidly
T10562 44838-44844 VB denotes expand
T10580 44845-44847 TO denotes to
T10581 44848-44855 VB denotes replace
T10582 44856-44859 DT denotes the
T10584 44860-44868 RB denotes normally
T10585 44869-44878 VBN denotes imprinted
T10583 44879-44884 NNS denotes cells
T10586 44885-44887 IN denotes in
T10587 44888-44902 JJ denotes extraembryonic
T10588 44903-44911 NNS denotes lineages
T10589 44912-44914 IN denotes if
T10591 44915-44918 DT denotes the
T10593 44919-44925 JJ denotes normal
T10592 44926-44935 NN denotes silencing
T10594 44936-44938 IN denotes of
T10595 44939-44941 NN denotes Xp
T10590 44942-44953 VBZ denotes compromises
T10596 44954-44958 NN denotes cell
T10597 44959-44965 NN denotes growth
T10598 44966-44968 CC denotes or
T10599 44969-44984 NN denotes differentiation
T10600 44984-44985 . denotes .
T10601 44985-45339 sentence denotes Interestingly, it has been suggested that the size of the population that initially escapes imprinting may range widely (from 0% to 30%), even between genetically identical embryos [37], and this may account for the variable phenotype observed among females bearing Xm-linked mutant alleles of genes essential for normal extraembryonic development [38].
T10602 44986-44999 RB denotes Interestingly
T10604 44999-45001 , denotes ,
T10605 45001-45003 PRP denotes it
T10606 45004-45007 VBZ denotes has
T10607 45008-45012 VBN denotes been
T10603 45013-45022 VBN denotes suggested
T10608 45023-45027 IN denotes that
T10610 45028-45031 DT denotes the
T10611 45032-45036 NN denotes size
T10612 45037-45039 IN denotes of
T10613 45040-45043 DT denotes the
T10614 45044-45054 NN denotes population
T10615 45055-45059 WDT denotes that
T10617 45060-45069 RB denotes initially
T10616 45070-45077 VBZ denotes escapes
T10618 45078-45088 NN denotes imprinting
T10619 45089-45092 MD denotes may
T10609 45093-45098 VB denotes range
T10620 45099-45105 RB denotes widely
T10621 45106-45107 -LRB- denotes (
T10622 45107-45111 IN denotes from
T10623 45112-45113 CD denotes 0
T10624 45113-45114 NN denotes %
T10625 45115-45117 IN denotes to
T10626 45118-45120 CD denotes 30
T10627 45120-45121 NN denotes %
T10628 45121-45122 -RRB- denotes )
T10629 45122-45124 , denotes ,
T10630 45124-45128 RB denotes even
T10631 45129-45136 IN denotes between
T10632 45137-45148 RB denotes genetically
T10633 45149-45158 JJ denotes identical
T10634 45159-45166 NNS denotes embryos
T10635 45167-45168 -LRB- denotes [
T10636 45168-45170 CD denotes 37
T10637 45170-45171 -RRB- denotes ]
T10638 45171-45173 , denotes ,
T10639 45173-45176 CC denotes and
T10640 45177-45181 DT denotes this
T10642 45182-45185 MD denotes may
T10641 45186-45193 VB denotes account
T10643 45194-45197 IN denotes for
T10644 45198-45201 DT denotes the
T10646 45202-45210 JJ denotes variable
T10645 45211-45220 NN denotes phenotype
T10647 45221-45229 VBN denotes observed
T10648 45230-45235 IN denotes among
T10649 45236-45243 NNS denotes females
T10650 45244-45251 VBG denotes bearing
T10651 45252-45254 NN denotes Xm
T10653 45254-45255 HYPH denotes -
T10652 45255-45261 VBN denotes linked
T10655 45262-45268 NN denotes mutant
T10654 45269-45276 NNS denotes alleles
T10656 45277-45279 IN denotes of
T10657 45280-45285 NNS denotes genes
T10658 45286-45295 JJ denotes essential
T10659 45296-45299 IN denotes for
T10660 45300-45306 JJ denotes normal
T10662 45307-45321 JJ denotes extraembryonic
T10661 45322-45333 NN denotes development
T10663 45334-45335 -LRB- denotes [
T10664 45335-45337 CD denotes 38
T10665 45337-45338 -RRB- denotes ]
T10666 45338-45339 . denotes .
T10667 45339-45492 sentence denotes Put simply, carrier females bearing a small initial population of escaping cells would be more severely affected than those bearing a larger population.
T10668 45340-45343 VBN denotes Put
T10670 45344-45350 RB denotes simply
T10671 45350-45352 , denotes ,
T10672 45352-45359 NN denotes carrier
T10673 45360-45367 NNS denotes females
T10674 45368-45375 VBG denotes bearing
T10675 45376-45377 DT denotes a
T10677 45378-45383 JJ denotes small
T10678 45384-45391 JJ denotes initial
T10676 45392-45402 NN denotes population
T10679 45403-45405 IN denotes of
T10680 45406-45414 VBG denotes escaping
T10681 45415-45420 NNS denotes cells
T10682 45421-45426 MD denotes would
T10669 45427-45429 VB denotes be
T10683 45430-45434 RBR denotes more
T10684 45435-45443 RB denotes severely
T10685 45444-45452 VBN denotes affected
T10686 45453-45457 IN denotes than
T10687 45458-45463 DT denotes those
T10688 45464-45471 VBG denotes bearing
T10689 45472-45473 DT denotes a
T10691 45474-45480 JJR denotes larger
T10690 45481-45491 NN denotes population
T10692 45491-45492 . denotes .
T10693 45492-45679 sentence denotes This could explain why we have observed significant phenotypic variation among Atrx carrier females, with some carriers dying in utero by 9.5 dpc (Table 1) and others developing to term.
T10694 45493-45497 DT denotes This
T10696 45498-45503 MD denotes could
T10695 45504-45511 VB denotes explain
T10697 45512-45515 WRB denotes why
T10699 45516-45518 PRP denotes we
T10700 45519-45523 VBP denotes have
T10698 45524-45532 VBN denotes observed
T10701 45533-45544 JJ denotes significant
T10703 45545-45555 JJ denotes phenotypic
T10702 45556-45565 NN denotes variation
T10704 45566-45571 IN denotes among
T10705 45572-45576 NN denotes Atrx
T10706 45577-45584 NN denotes carrier
T10707 45585-45592 NNS denotes females
T10708 45592-45594 , denotes ,
T10709 45594-45598 IN denotes with
T10710 45599-45603 DT denotes some
T10711 45604-45612 NNS denotes carriers
T10712 45613-45618 VBG denotes dying
T10713 45619-45621 FW denotes in
T10714 45622-45627 FW denotes utero
T10715 45628-45630 IN denotes by
T10716 45631-45634 CD denotes 9.5
T10717 45635-45638 NNS denotes dpc
T10718 45639-45640 -LRB- denotes (
T10719 45640-45645 NN denotes Table
T10720 45646-45647 CD denotes 1
T10721 45647-45648 -RRB- denotes )
T10722 45649-45652 CC denotes and
T10723 45653-45659 NNS denotes others
T10724 45660-45670 VBG denotes developing
T10725 45671-45673 IN denotes to
T10726 45674-45678 NN denotes term
T10727 45678-45679 . denotes .
T10728 45679-45848 sentence denotes Another possible mechanism is that inactivation of the paternal X proceeds normally in all cells, but subsequently the Atrx gene within individual cells is reactivated.
T10729 45680-45687 DT denotes Another
T10731 45688-45696 JJ denotes possible
T10730 45697-45706 NN denotes mechanism
T10732 45707-45709 VBZ denotes is
T10733 45710-45714 IN denotes that
T10735 45715-45727 NN denotes inactivation
T10736 45728-45730 IN denotes of
T10737 45731-45734 DT denotes the
T10739 45735-45743 JJ denotes paternal
T10738 45744-45745 NN denotes X
T10734 45746-45754 VBZ denotes proceeds
T10740 45755-45763 RB denotes normally
T10741 45764-45766 IN denotes in
T10742 45767-45770 DT denotes all
T10743 45771-45776 NNS denotes cells
T10744 45776-45778 , denotes ,
T10745 45778-45781 CC denotes but
T10746 45782-45794 RB denotes subsequently
T10748 45795-45798 DT denotes the
T10750 45799-45803 NN denotes Atrx
T10749 45804-45808 NN denotes gene
T10751 45809-45815 IN denotes within
T10752 45816-45826 JJ denotes individual
T10753 45827-45832 NNS denotes cells
T10754 45833-45835 VBZ denotes is
T10747 45836-45847 VBN denotes reactivated
T10755 45847-45848 . denotes .
T10756 45848-45961 sentence denotes Alternatively, in the absence of Atrx, the paternal allele may partially escape the normal process of silencing.
T10757 45849-45862 RB denotes Alternatively
T10759 45862-45864 , denotes ,
T10760 45864-45866 IN denotes in
T10761 45867-45870 DT denotes the
T10762 45871-45878 NN denotes absence
T10763 45879-45881 IN denotes of
T10764 45882-45886 NN denotes Atrx
T10765 45886-45888 , denotes ,
T10766 45888-45891 DT denotes the
T10768 45892-45900 JJ denotes paternal
T10767 45901-45907 NN denotes allele
T10769 45908-45911 MD denotes may
T10770 45912-45921 RB denotes partially
T10758 45922-45928 VB denotes escape
T10771 45929-45932 DT denotes the
T10773 45933-45939 JJ denotes normal
T10772 45940-45947 NN denotes process
T10774 45948-45950 IN denotes of
T10775 45951-45960 NN denotes silencing
T10776 45960-45961 . denotes .
T10777 45961-46215 sentence denotes In both of these cases, other genes on the paternal X chromosome must be inactivated and remain so, since blocking inactivation of the entire Xp chromosome causes embryonic lethality due to biallelic expression of X-linked genes in the trophoblast [39].
T10778 45962-45964 IN denotes In
T10780 45965-45969 DT denotes both
T10781 45970-45972 IN denotes of
T10782 45973-45978 DT denotes these
T10783 45979-45984 NNS denotes cases
T10784 45984-45986 , denotes ,
T10785 45986-45991 JJ denotes other
T10786 45992-45997 NNS denotes genes
T10787 45998-46000 IN denotes on
T10788 46001-46004 DT denotes the
T10790 46005-46013 JJ denotes paternal
T10791 46014-46015 NN denotes X
T10789 46016-46026 NN denotes chromosome
T10792 46027-46031 MD denotes must
T10793 46032-46034 VB denotes be
T10779 46035-46046 VBN denotes inactivated
T10794 46047-46050 CC denotes and
T10795 46051-46057 VBP denotes remain
T10796 46058-46060 RB denotes so
T10797 46060-46062 , denotes ,
T10798 46062-46067 IN denotes since
T10800 46068-46076 VBG denotes blocking
T10801 46077-46089 NN denotes inactivation
T10802 46090-46092 IN denotes of
T10803 46093-46096 DT denotes the
T10805 46097-46103 JJ denotes entire
T10806 46104-46106 NN denotes Xp
T10804 46107-46117 NN denotes chromosome
T10799 46118-46124 VBZ denotes causes
T10807 46125-46134 JJ denotes embryonic
T10808 46135-46144 NN denotes lethality
T10809 46145-46148 IN denotes due
T10810 46149-46151 IN denotes to
T10811 46152-46161 JJ denotes biallelic
T10812 46162-46172 NN denotes expression
T10813 46173-46175 IN denotes of
T10814 46176-46177 NN denotes X
T10816 46177-46178 HYPH denotes -
T10815 46178-46184 VBN denotes linked
T10817 46185-46190 NNS denotes genes
T10818 46191-46193 IN denotes in
T10819 46194-46197 DT denotes the
T10820 46198-46209 NN denotes trophoblast
T10821 46210-46211 -LRB- denotes [
T10822 46211-46213 CD denotes 39
T10823 46213-46214 -RRB- denotes ]
T10824 46214-46215 . denotes .
T10925 46217-46224 NN denotes Summary
T10926 46224-46341 sentence denotes ATR-X syndrome is the first human genetic disease known to be caused by mutations in a chromatin remodelling factor.
T10927 46225-46228 NN denotes ATR
T10929 46228-46229 HYPH denotes -
T10928 46229-46230 NN denotes X
T10930 46231-46239 NN denotes syndrome
T10931 46240-46242 VBZ denotes is
T10932 46243-46246 DT denotes the
T10934 46247-46252 JJ denotes first
T10935 46253-46258 JJ denotes human
T10936 46259-46266 JJ denotes genetic
T10933 46267-46274 NN denotes disease
T10937 46275-46280 VBN denotes known
T10938 46281-46283 TO denotes to
T10940 46284-46286 VB denotes be
T10939 46287-46293 VBN denotes caused
T10941 46294-46296 IN denotes by
T10942 46297-46306 NNS denotes mutations
T10943 46307-46309 IN denotes in
T10944 46310-46311 DT denotes a
T10946 46312-46321 NN denotes chromatin
T10947 46322-46333 NN denotes remodelling
T10945 46334-46340 NN denotes factor
T10948 46340-46341 . denotes .
T10949 46341-46444 sentence denotes At present we do not know how ATRX influences gene expression or what effect it has on cell behaviour.
T10950 46342-46344 IN denotes At
T10952 46345-46352 JJ denotes present
T10953 46353-46355 PRP denotes we
T10954 46356-46358 VBP denotes do
T10955 46359-46362 RB denotes not
T10951 46363-46367 VB denotes know
T10956 46368-46371 WRB denotes how
T10958 46372-46376 NN denotes ATRX
T10957 46377-46387 VBZ denotes influences
T10959 46388-46392 NN denotes gene
T10960 46393-46403 NN denotes expression
T10961 46404-46406 CC denotes or
T10962 46407-46411 WDT denotes what
T10963 46412-46418 NN denotes effect
T10965 46419-46421 PRP denotes it
T10964 46422-46425 VBZ denotes has
T10966 46426-46428 IN denotes on
T10967 46429-46433 NN denotes cell
T10968 46434-46443 NN denotes behaviour
T10969 46443-46444 . denotes .
T10970 46444-46620 sentence denotes Nevertheless, we have previously noted that none of the natural mutations causing ATR-X syndrome are nulls, which suggests that it plays a critical role in normal development.
T10971 46445-46457 RB denotes Nevertheless
T10973 46457-46459 , denotes ,
T10974 46459-46461 PRP denotes we
T10975 46462-46466 VBP denotes have
T10976 46467-46477 RB denotes previously
T10972 46478-46483 VBN denotes noted
T10977 46484-46488 IN denotes that
T10979 46489-46493 NN denotes none
T10980 46494-46496 IN denotes of
T10981 46497-46500 DT denotes the
T10983 46501-46508 JJ denotes natural
T10982 46509-46518 NNS denotes mutations
T10984 46519-46526 VBG denotes causing
T10985 46527-46530 NN denotes ATR
T10987 46530-46531 HYPH denotes -
T10986 46531-46532 NN denotes X
T10988 46533-46541 NN denotes syndrome
T10978 46542-46545 VBP denotes are
T10989 46546-46551 NNS denotes nulls
T10990 46551-46553 , denotes ,
T10991 46553-46558 WDT denotes which
T10992 46559-46567 VBZ denotes suggests
T10993 46568-46572 IN denotes that
T10995 46573-46575 PRP denotes it
T10994 46576-46581 VBZ denotes plays
T10996 46582-46583 DT denotes a
T10998 46584-46592 JJ denotes critical
T10997 46593-46597 NN denotes role
T10999 46598-46600 IN denotes in
T11000 46601-46607 JJ denotes normal
T11001 46608-46619 NN denotes development
T11002 46619-46620 . denotes .
T11003 46620-46822 sentence denotes Results of conditional inactivation of Atrx in the developing mouse forebrain, based on the Atrx flox allele described here, shows that Atrx exerts a major effect on terminally differentiating neurons.
T11004 46621-46628 NNS denotes Results
T11006 46629-46631 IN denotes of
T11007 46632-46643 JJ denotes conditional
T11008 46644-46656 NN denotes inactivation
T11009 46657-46659 IN denotes of
T11010 46660-46664 NN denotes Atrx
T11011 46665-46667 IN denotes in
T11012 46668-46671 DT denotes the
T11014 46672-46682 VBG denotes developing
T11015 46683-46688 NN denotes mouse
T11013 46689-46698 NN denotes forebrain
T11016 46698-46700 , denotes ,
T11017 46700-46705 VBN denotes based
T11018 46706-46708 IN denotes on
T11019 46709-46712 DT denotes the
T11021 46713-46722 NN denotes Atrx flox
T11020 46723-46729 NN denotes allele
T11022 46730-46739 VBN denotes described
T11023 46740-46744 RB denotes here
T11024 46744-46746 , denotes ,
T11005 46746-46751 VBZ denotes shows
T11025 46752-46756 IN denotes that
T11027 46757-46761 NN denotes Atrx
T11026 46762-46768 VBZ denotes exerts
T11028 46769-46770 DT denotes a
T11030 46771-46776 JJ denotes major
T11029 46777-46783 NN denotes effect
T11031 46784-46786 IN denotes on
T11032 46787-46797 RB denotes terminally
T11033 46798-46813 VBG denotes differentiating
T11034 46814-46821 NNS denotes neurons
T11035 46821-46822 . denotes .
T11036 46822-46885 sentence denotes Conditional inactivation of Atrx in other tissues is underway.
T11037 46823-46834 JJ denotes Conditional
T11038 46835-46847 NN denotes inactivation
T11040 46848-46850 IN denotes of
T11041 46851-46855 NN denotes Atrx
T11042 46856-46858 IN denotes in
T11043 46859-46864 JJ denotes other
T11044 46865-46872 NNS denotes tissues
T11039 46873-46875 VBZ denotes is
T11045 46876-46884 JJ denotes underway
T11046 46884-46885 . denotes .
T11047 46885-47074 sentence denotes Here we have shown that animal-wide disruption of the Atrx gene causes a severe embryonic-lethal phenotype, revealing an essential role for Atrx in the formation of the murine trophoblast.
T11048 46886-46890 RB denotes Here
T11050 46891-46893 PRP denotes we
T11051 46894-46898 VBP denotes have
T11049 46899-46904 VBN denotes shown
T11052 46905-46909 IN denotes that
T11054 46910-46916 NN denotes animal
T11056 46916-46917 HYPH denotes -
T11055 46917-46921 JJ denotes wide
T11057 46922-46932 NN denotes disruption
T11058 46933-46935 IN denotes of
T11059 46936-46939 DT denotes the
T11061 46940-46944 NN denotes Atrx
T11060 46945-46949 NN denotes gene
T11053 46950-46956 VBZ denotes causes
T11062 46957-46958 DT denotes a
T11064 46959-46965 JJ denotes severe
T11065 46966-46975 JJ denotes embryonic
T11067 46975-46976 HYPH denotes -
T11066 46976-46982 JJ denotes lethal
T11063 46983-46992 NN denotes phenotype
T11068 46992-46994 , denotes ,
T11069 46994-47003 VBG denotes revealing
T11070 47004-47006 DT denotes an
T11072 47007-47016 JJ denotes essential
T11071 47017-47021 NN denotes role
T11073 47022-47025 IN denotes for
T11074 47026-47030 NN denotes Atrx
T11075 47031-47033 IN denotes in
T11076 47034-47037 DT denotes the
T11077 47038-47047 NN denotes formation
T11078 47048-47050 IN denotes of
T11079 47051-47054 DT denotes the
T11081 47055-47061 JJ denotes murine
T11080 47062-47073 NN denotes trophoblast
T11082 47073-47074 . denotes .
T11083 47074-47205 sentence denotes In addition, Atrx appears to escape imprinted X-chromosome inactivation in the extraembryonic tissues of some carrier female mice.
T11084 47075-47077 IN denotes In
T11086 47078-47086 NN denotes addition
T11087 47086-47088 , denotes ,
T11088 47088-47092 NN denotes Atrx
T11085 47093-47100 VBZ denotes appears
T11089 47101-47103 TO denotes to
T11090 47104-47110 VB denotes escape
T11091 47111-47120 VBN denotes imprinted
T11093 47121-47122 NN denotes X
T11094 47122-47123 HYPH denotes -
T11092 47123-47133 NN denotes chromosome
T11095 47134-47146 NN denotes inactivation
T11096 47147-47149 IN denotes in
T11097 47150-47153 DT denotes the
T11099 47154-47168 JJ denotes extraembryonic
T11098 47169-47176 NNS denotes tissues
T11100 47177-47179 IN denotes of
T11101 47180-47184 DT denotes some
T11103 47185-47192 NN denotes carrier
T11104 47193-47199 JJ denotes female
T11102 47200-47204 NNS denotes mice
T11105 47204-47205 . denotes .
T11189 47230-47240 NN denotes Generation
T11190 47241-47243 IN denotes of
T11191 47244-47246 NN denotes ES
T11192 47247-47252 NNS denotes cells
T11193 47253-47260 VBG denotes bearing
T11194 47261-47264 DT denotes the
T11196 47265-47274 NN denotes Atrx flox
T11195 47275-47281 NN denotes allele
T11197 47281-47282 . denotes .
T11198 47282-47447 sentence denotes Briefly, the targeting vector (shown in Figure 2A) places a loxP site within intron 18 and a loxP-flanked MC1neopA selection cassette in intron 17 of the Atrx gene.
T11199 47283-47290 RB denotes Briefly
T11201 47290-47292 , denotes ,
T11202 47292-47295 DT denotes the
T11204 47296-47305 NN denotes targeting
T11203 47306-47312 NN denotes vector
T11205 47313-47314 -LRB- denotes (
T11206 47314-47319 VBN denotes shown
T11207 47320-47322 IN denotes in
T11208 47323-47329 NN denotes Figure
T11209 47330-47332 NN denotes 2A
T11210 47332-47333 -RRB- denotes )
T11200 47334-47340 VBZ denotes places
T11211 47341-47342 DT denotes a
T11213 47343-47347 NN denotes loxP
T11212 47348-47352 NN denotes site
T11214 47353-47359 IN denotes within
T11215 47360-47366 NN denotes intron
T11216 47367-47369 CD denotes 18
T11217 47370-47373 CC denotes and
T11218 47374-47375 DT denotes a
T11220 47376-47380 NN denotes loxP
T11222 47380-47381 HYPH denotes -
T11221 47381-47388 VBN denotes flanked
T11223 47389-47397 NN denotes MC1neopA
T11224 47398-47407 NN denotes selection
T11219 47408-47416 NN denotes cassette
T11225 47417-47419 IN denotes in
T11226 47420-47426 NN denotes intron
T11227 47427-47429 CD denotes 17
T11228 47430-47432 IN denotes of
T11229 47433-47436 DT denotes the
T11231 47437-47441 NN denotes Atrx
T11230 47442-47446 NN denotes gene
T11232 47446-47447 . denotes .
T11233 47447-47518 sentence denotes A detailed description of the targeting construct is provided in [20].
T11234 47448-47449 DT denotes A
T11236 47450-47458 VBN denotes detailed
T11235 47459-47470 NN denotes description
T11238 47471-47473 IN denotes of
T11239 47474-47477 DT denotes the
T11241 47478-47487 NN denotes targeting
T11240 47488-47497 NN denotes construct
T11242 47498-47500 VBZ denotes is
T11237 47501-47509 VBN denotes provided
T11243 47510-47512 IN denotes in
T11244 47513-47514 -LRB- denotes [
T11245 47514-47516 CD denotes 20
T11246 47516-47517 -RRB- denotes ]
T11247 47517-47518 . denotes .
T11248 47518-47658 sentence denotes Linearised plasmid (150 μg) was electroporated into 1 × 108 E14Tg2a ES cells, and colonies resistant to G418 and ganciclovir were isolated.
T11249 47519-47529 VBN denotes Linearised
T11250 47530-47537 NN denotes plasmid
T11252 47538-47539 -LRB- denotes (
T11254 47539-47542 CD denotes 150
T11253 47543-47545 NNS denotes μg
T11255 47545-47546 -RRB- denotes )
T11256 47547-47550 VBD denotes was
T11251 47551-47565 VBN denotes electroporated
T11257 47566-47570 IN denotes into
T11258 47571-47572 CD denotes 1
T11260 47573-47574 SYM denotes ×
T11259 47575-47578 CD denotes 108
T11262 47579-47586 NN denotes E14Tg2a
T11263 47587-47589 NN denotes ES
T11261 47590-47595 NNS denotes cells
T11264 47595-47597 , denotes ,
T11265 47597-47600 CC denotes and
T11266 47601-47609 NNS denotes colonies
T11268 47610-47619 JJ denotes resistant
T11269 47620-47622 IN denotes to
T11270 47623-47627 NN denotes G418
T11271 47628-47631 CC denotes and
T11272 47632-47643 NN denotes ganciclovir
T11273 47644-47648 VBD denotes were
T11267 47649-47657 VBN denotes isolated
T11274 47657-47658 . denotes .
T11275 47658-47892 sentence denotes Homologous targeting events were identified by Southern blot of EcoRI-digested DNA and hybridisation with a 5′ probe (generated with primers PPS1.20 and PPS1.27) and a 3′ probe (a 0.9-kb HaeIII fragment) as shown in Figure 2A and 2B.
T11276 47659-47669 JJ denotes Homologous
T11278 47670-47679 NN denotes targeting
T11277 47680-47686 NNS denotes events
T11280 47687-47691 VBD denotes were
T11279 47692-47702 VBN denotes identified
T11281 47703-47705 IN denotes by
T11282 47706-47714 NNP denotes Southern
T11283 47715-47719 NN denotes blot
T11284 47720-47722 IN denotes of
T11285 47723-47728 NN denotes EcoRI
T11287 47728-47729 HYPH denotes -
T11286 47729-47737 VBN denotes digested
T11288 47738-47741 NN denotes DNA
T11289 47742-47745 CC denotes and
T11290 47746-47759 NN denotes hybridisation
T11291 47760-47764 IN denotes with
T11292 47765-47766 DT denotes a
T11294 47767-47768 CD denotes 5
T11295 47768-47769 SYM denotes ′
T11293 47770-47775 NN denotes probe
T11296 47776-47777 -LRB- denotes (
T11297 47777-47786 VBN denotes generated
T11298 47787-47791 IN denotes with
T11299 47792-47799 NNS denotes primers
T11300 47800-47807 NN denotes PPS1.20
T11301 47808-47811 CC denotes and
T11302 47812-47819 NN denotes PPS1.27
T11303 47819-47820 -RRB- denotes )
T11304 47821-47824 CC denotes and
T11305 47825-47826 DT denotes a
T11307 47827-47828 CD denotes 3
T11308 47828-47829 SYM denotes ′
T11306 47830-47835 NN denotes probe
T11309 47836-47837 -LRB- denotes (
T11310 47837-47838 DT denotes a
T11312 47839-47842 CD denotes 0.9
T11314 47842-47843 HYPH denotes -
T11313 47843-47845 NN denotes kb
T11315 47846-47852 NN denotes HaeIII
T11311 47853-47861 NN denotes fragment
T11316 47861-47862 -RRB- denotes )
T11317 47863-47865 IN denotes as
T11318 47866-47871 VBN denotes shown
T11319 47872-47874 IN denotes in
T11320 47875-47881 NN denotes Figure
T11321 47882-47884 NN denotes 2A
T11322 47885-47888 CC denotes and
T11323 47889-47891 NN denotes 2B
T11324 47891-47892 . denotes .
T11325 47892-48211 sentence denotes DNA from correctly targeted clones was also digested with SacI and analysed by Southern blot and hybridisation with a probe from within intron 17 (a PCR product generated with primers PPS1.15 and Xnp46) to confirm that the loxP site within intron 18 had been included within the crossed-over region (Figure 2A and 2C).
T11326 47893-47896 NN denotes DNA
T11328 47897-47901 IN denotes from
T11329 47902-47911 RB denotes correctly
T11330 47912-47920 VBN denotes targeted
T11331 47921-47927 NNS denotes clones
T11332 47928-47931 VBD denotes was
T11333 47932-47936 RB denotes also
T11327 47937-47945 VBN denotes digested
T11334 47946-47950 IN denotes with
T11335 47951-47955 NN denotes SacI
T11336 47956-47959 CC denotes and
T11337 47960-47968 VBN denotes analysed
T11338 47969-47971 IN denotes by
T11339 47972-47980 NNP denotes Southern
T11340 47981-47985 NN denotes blot
T11341 47986-47989 CC denotes and
T11342 47990-48003 NN denotes hybridisation
T11343 48004-48008 IN denotes with
T11344 48009-48010 DT denotes a
T11345 48011-48016 NN denotes probe
T11346 48017-48021 IN denotes from
T11347 48022-48028 IN denotes within
T11348 48029-48035 NN denotes intron
T11349 48036-48038 CD denotes 17
T11350 48039-48040 -LRB- denotes (
T11351 48040-48041 DT denotes a
T11353 48042-48045 NN denotes PCR
T11352 48046-48053 NN denotes product
T11354 48054-48063 VBN denotes generated
T11355 48064-48068 IN denotes with
T11356 48069-48076 NNS denotes primers
T11357 48077-48084 NN denotes PPS1.15
T11358 48085-48088 CC denotes and
T11359 48089-48094 NN denotes Xnp46
T11360 48094-48095 -RRB- denotes )
T11361 48096-48098 TO denotes to
T11362 48099-48106 VB denotes confirm
T11363 48107-48111 IN denotes that
T11365 48112-48115 DT denotes the
T11367 48116-48120 NN denotes loxP
T11366 48121-48125 NN denotes site
T11368 48126-48132 IN denotes within
T11369 48133-48139 NN denotes intron
T11370 48140-48142 CD denotes 18
T11371 48143-48146 VBD denotes had
T11372 48147-48151 VBN denotes been
T11364 48152-48160 VBN denotes included
T11373 48161-48167 IN denotes within
T11374 48168-48171 DT denotes the
T11376 48172-48179 VBN denotes crossed
T11377 48179-48180 HYPH denotes -
T11378 48180-48184 RP denotes over
T11375 48185-48191 NN denotes region
T11379 48192-48193 -LRB- denotes (
T11381 48193-48199 NN denotes Figure
T11380 48200-48202 NN denotes 2A
T11382 48203-48206 CC denotes and
T11383 48207-48209 NN denotes 2C
T11384 48209-48210 -RRB- denotes )
T11385 48210-48211 . denotes .
T11386 48211-48255 sentence denotes Sequences of primers are shown in Table S1.
T11387 48212-48221 NNS denotes Sequences
T11389 48222-48224 IN denotes of
T11390 48225-48232 NNS denotes primers
T11391 48233-48236 VBP denotes are
T11388 48237-48242 VBN denotes shown
T11392 48243-48245 IN denotes in
T11393 48246-48251 NN denotes Table
T11394 48252-48254 NN denotes S1
T11395 48254-48255 . denotes .
T11569 48257-48260 NN denotes Cre
T11571 48260-48261 HYPH denotes -
T11570 48261-48274 NN denotes recombination
T11572 48275-48278 CC denotes and
T11573 48279-48295 NN denotes characterisation
T11574 48296-48298 IN denotes of
T11575 48299-48307 JJ denotes Atrxnull
T11577 48308-48310 NN denotes ES
T11576 48311-48316 NNS denotes cells
T11578 48317-48320 CC denotes and
T11579 48321-48329 JJ denotes embryoid
T11580 48330-48336 NNS denotes bodies
T11581 48336-48337 . denotes .
T11582 48337-48497 sentence denotes ES cell clones bearing the Atrx flox allele (1 × 107 cells) were transiently transfected with 50 μg of uncut Cre expression plasmid (pCAGGS-Cre-IRESpuro) [40].
T11583 48338-48340 NN denotes ES
T11584 48341-48345 NN denotes cell
T11585 48346-48352 NNS denotes clones
T11587 48353-48360 VBG denotes bearing
T11588 48361-48364 DT denotes the
T11590 48365-48374 NN denotes Atrx flox
T11589 48375-48381 NN denotes allele
T11591 48382-48383 -LRB- denotes (
T11593 48383-48384 CD denotes 1
T11595 48385-48386 SYM denotes ×
T11594 48387-48390 CD denotes 107
T11592 48391-48396 NNS denotes cells
T11596 48396-48397 -RRB- denotes )
T11597 48398-48402 VBD denotes were
T11598 48403-48414 RB denotes transiently
T11586 48415-48426 VBN denotes transfected
T11599 48427-48431 IN denotes with
T11600 48432-48434 CD denotes 50
T11601 48435-48437 NNS denotes μg
T11602 48438-48440 IN denotes of
T11603 48441-48446 JJ denotes uncut
T11605 48447-48450 NN denotes Cre
T11606 48451-48461 NN denotes expression
T11604 48462-48469 NN denotes plasmid
T11607 48470-48471 -LRB- denotes (
T11608 48471-48477 NN denotes pCAGGS
T11610 48477-48478 HYPH denotes -
T11611 48478-48481 NN denotes Cre
T11612 48481-48482 HYPH denotes -
T11609 48482-48490 NN denotes IRESpuro
T11613 48490-48491 -RRB- denotes )
T11614 48492-48493 -LRB- denotes [
T11615 48493-48495 CD denotes 40
T11616 48495-48496 -RRB- denotes ]
T11617 48496-48497 . denotes .
T11618 48497-48649 sentence denotes Following transfection, cells were plated at a range of clonal densities in complete medium without G418, and isolated subclones were picked after 7 d.
T11619 48498-48507 VBG denotes Following
T11621 48508-48520 NN denotes transfection
T11622 48520-48522 , denotes ,
T11623 48522-48527 NNS denotes cells
T11624 48528-48532 VBD denotes were
T11620 48533-48539 VBN denotes plated
T11625 48540-48542 IN denotes at
T11626 48543-48544 DT denotes a
T11627 48545-48550 NN denotes range
T11628 48551-48553 IN denotes of
T11629 48554-48560 JJ denotes clonal
T11630 48561-48570 NNS denotes densities
T11631 48571-48573 IN denotes in
T11632 48574-48582 JJ denotes complete
T11633 48583-48589 NN denotes medium
T11634 48590-48597 IN denotes without
T11635 48598-48602 NN denotes G418
T11636 48602-48604 , denotes ,
T11637 48604-48607 CC denotes and
T11638 48608-48616 VBN denotes isolated
T11639 48617-48626 NNS denotes subclones
T11641 48627-48631 VBD denotes were
T11640 48632-48638 VBN denotes picked
T11642 48639-48644 IN denotes after
T11643 48645-48646 CD denotes 7
T11644 48647-48648 NNS denotes d
T11645 48648-48649 . denotes .
T11646 48649-48894 sentence denotes Subclones were expanded and analysed for the presence of a recombinant locus initially by PCR, to detect deletion of the MC1neopA cassette, and then by Southern blot and hybridisation with the intron 17 probe described above (Figure 2A and 2C).
T11647 48650-48659 NNS denotes Subclones
T11649 48660-48664 VBD denotes were
T11648 48665-48673 VBN denotes expanded
T11650 48674-48677 CC denotes and
T11651 48678-48686 VBN denotes analysed
T11652 48687-48690 IN denotes for
T11653 48691-48694 DT denotes the
T11654 48695-48703 NN denotes presence
T11655 48704-48706 IN denotes of
T11656 48707-48708 DT denotes a
T11658 48709-48720 JJ denotes recombinant
T11657 48721-48726 NN denotes locus
T11659 48727-48736 RB denotes initially
T11660 48737-48739 IN denotes by
T11661 48740-48743 NN denotes PCR
T11662 48743-48745 , denotes ,
T11663 48745-48747 TO denotes to
T11664 48748-48754 VB denotes detect
T11665 48755-48763 NN denotes deletion
T11666 48764-48766 IN denotes of
T11667 48767-48770 DT denotes the
T11669 48771-48779 NN denotes MC1neopA
T11668 48780-48788 NN denotes cassette
T11670 48788-48790 , denotes ,
T11671 48790-48793 CC denotes and
T11672 48794-48798 RB denotes then
T11673 48799-48801 IN denotes by
T11674 48802-48810 NNP denotes Southern
T11675 48811-48815 NN denotes blot
T11676 48816-48819 CC denotes and
T11677 48820-48833 NN denotes hybridisation
T11678 48834-48838 IN denotes with
T11679 48839-48842 DT denotes the
T11681 48843-48849 NN denotes intron
T11682 48850-48852 CD denotes 17
T11680 48853-48858 NN denotes probe
T11683 48859-48868 VBN denotes described
T11684 48869-48874 RB denotes above
T11685 48875-48876 -LRB- denotes (
T11687 48876-48882 NN denotes Figure
T11686 48883-48885 NN denotes 2A
T11688 48886-48889 CC denotes and
T11689 48890-48892 NN denotes 2C
T11690 48892-48893 -RRB- denotes )
T11691 48893-48894 . denotes .
T11692 48894-49064 sentence denotes Northern blots were carried out according to standard techniques using 20 μg of total RNA isolated using TRI Reagent (Sigma-Aldrich, St. Louis, Missouri, United States).
T11693 48895-48903 NNP denotes Northern
T11694 48904-48909 NNS denotes blots
T11696 48910-48914 VBD denotes were
T11695 48915-48922 VBN denotes carried
T11697 48923-48926 RP denotes out
T11698 48927-48936 VBG denotes according
T11699 48937-48939 IN denotes to
T11700 48940-48948 JJ denotes standard
T11701 48949-48959 NNS denotes techniques
T11702 48960-48965 VBG denotes using
T11703 48966-48968 CD denotes 20
T11704 48969-48971 NNS denotes μg
T11705 48972-48974 IN denotes of
T11706 48975-48980 JJ denotes total
T11707 48981-48984 NN denotes RNA
T11708 48985-48993 VBN denotes isolated
T11709 48994-48999 VBG denotes using
T11710 49000-49003 NN denotes TRI
T11711 49004-49011 NN denotes Reagent
T11712 49012-49013 -LRB- denotes (
T11714 49013-49018 NNP denotes Sigma
T11715 49018-49019 HYPH denotes -
T11713 49019-49026 NNP denotes Aldrich
T11716 49026-49028 , denotes ,
T11717 49028-49031 NNP denotes St.
T11718 49032-49037 NNP denotes Louis
T11719 49037-49039 , denotes ,
T11720 49039-49047 NNP denotes Missouri
T11721 49047-49049 , denotes ,
T11722 49049-49055 NNP denotes United
T11723 49056-49062 NNP denotes States
T11724 49062-49063 -RRB- denotes )
T11725 49063-49064 . denotes .
T11726 49064-49192 sentence denotes The blot was hybridised with a probe from within exon 10 of the Atrx gene (generated with primers Mxnp4 and Mxnp28 [Table S1]).
T11727 49065-49068 DT denotes The
T11728 49069-49073 NN denotes blot
T11730 49074-49077 VBD denotes was
T11729 49078-49088 VBN denotes hybridised
T11731 49089-49093 IN denotes with
T11732 49094-49095 DT denotes a
T11733 49096-49101 NN denotes probe
T11734 49102-49106 IN denotes from
T11735 49107-49113 IN denotes within
T11736 49114-49118 NN denotes exon
T11737 49119-49121 CD denotes 10
T11738 49122-49124 IN denotes of
T11739 49125-49128 DT denotes the
T11741 49129-49133 NN denotes Atrx
T11740 49134-49138 NN denotes gene
T11742 49139-49140 -LRB- denotes (
T11743 49140-49149 VBN denotes generated
T11744 49150-49154 IN denotes with
T11745 49155-49162 NNS denotes primers
T11746 49163-49168 NN denotes Mxnp4
T11747 49169-49172 CC denotes and
T11748 49173-49179 NN denotes Mxnp28
T11749 49180-49181 -LRB- denotes [
T11751 49181-49186 NN denotes Table
T11750 49187-49189 NN denotes S1
T11752 49189-49190 -RRB- denotes ]
T11753 49190-49191 -RRB- denotes )
T11754 49191-49192 . denotes .
T11755 49192-49319 sentence denotes After it was stripped, the membrane was hybridised with a β-actin cDNA probe (Clontech, Palo Alto, California, United States).
T11756 49193-49198 IN denotes After
T11758 49199-49201 PRP denotes it
T11759 49202-49205 VBD denotes was
T11757 49206-49214 VBN denotes stripped
T11761 49214-49216 , denotes ,
T11762 49216-49219 DT denotes the
T11763 49220-49228 NN denotes membrane
T11764 49229-49232 VBD denotes was
T11760 49233-49243 VBN denotes hybridised
T11765 49244-49248 IN denotes with
T11766 49249-49250 DT denotes a
T11768 49251-49252 NN denotes β
T11770 49252-49253 HYPH denotes -
T11769 49253-49258 NN denotes actin
T11771 49259-49263 NN denotes cDNA
T11767 49264-49269 NN denotes probe
T11772 49270-49271 -LRB- denotes (
T11773 49271-49279 NNP denotes Clontech
T11774 49279-49281 , denotes ,
T11775 49281-49285 NNP denotes Palo
T11776 49286-49290 NNP denotes Alto
T11777 49290-49292 , denotes ,
T11778 49292-49302 NNP denotes California
T11779 49302-49304 , denotes ,
T11780 49304-49310 NNP denotes United
T11781 49311-49317 NNP denotes States
T11782 49317-49318 -RRB- denotes )
T11783 49318-49319 . denotes .
T11784 49319-49479 sentence denotes Protein extraction and detection of Atrx by Western blotting was performed as described previously [4], using the mouse monoclonal anti-ATRX antibody 23C [15].
T11785 49320-49327 NN denotes Protein
T11786 49328-49338 NN denotes extraction
T11788 49339-49342 CC denotes and
T11789 49343-49352 NN denotes detection
T11790 49353-49355 IN denotes of
T11791 49356-49360 NN denotes Atrx
T11792 49361-49363 IN denotes by
T11793 49364-49371 NNP denotes Western
T11794 49372-49380 NN denotes blotting
T11795 49381-49384 VBD denotes was
T11787 49385-49394 VBN denotes performed
T11796 49395-49397 IN denotes as
T11797 49398-49407 VBN denotes described
T11798 49408-49418 RB denotes previously
T11799 49419-49420 -LRB- denotes [
T11800 49420-49421 CD denotes 4
T11801 49421-49422 -RRB- denotes ]
T11802 49422-49424 , denotes ,
T11803 49424-49429 VBG denotes using
T11804 49430-49433 DT denotes the
T11806 49434-49439 NN denotes mouse
T11807 49440-49450 JJ denotes monoclonal
T11808 49451-49460 JJ denotes anti-ATRX
T11805 49461-49469 NN denotes antibody
T11809 49470-49473 NN denotes 23C
T11810 49474-49475 -LRB- denotes [
T11811 49475-49477 CD denotes 15
T11812 49477-49478 -RRB- denotes ]
T11813 49478-49479 . denotes .
T11814 49479-49546 sentence denotes Analyses of cell cycle and apoptosis are described in Protocol S1.
T11815 49480-49488 NNS denotes Analyses
T11817 49489-49491 IN denotes of
T11818 49492-49496 NN denotes cell
T11819 49497-49502 NN denotes cycle
T11820 49503-49506 CC denotes and
T11821 49507-49516 NN denotes apoptosis
T11822 49517-49520 VBP denotes are
T11816 49521-49530 VBN denotes described
T11823 49531-49533 IN denotes in
T11824 49534-49542 NN denotes Protocol
T11825 49543-49545 NN denotes S1
T11826 49545-49546 . denotes .
T11827 49546-49708 sentence denotes Methylation of rDNA was analysed in DNA from ES cell clones or from embryoid bodies recovered after 7 d of in vitro differentiation as described previously [41].
T11828 49547-49558 NN denotes Methylation
T11830 49559-49561 IN denotes of
T11831 49562-49566 NN denotes rDNA
T11832 49567-49570 VBD denotes was
T11829 49571-49579 VBN denotes analysed
T11833 49580-49582 IN denotes in
T11834 49583-49586 NN denotes DNA
T11835 49587-49591 IN denotes from
T11836 49592-49594 NN denotes ES
T11837 49595-49599 NN denotes cell
T11838 49600-49606 NNS denotes clones
T11839 49607-49609 CC denotes or
T11840 49610-49614 IN denotes from
T11841 49615-49623 JJ denotes embryoid
T11842 49624-49630 NNS denotes bodies
T11843 49631-49640 VBN denotes recovered
T11844 49641-49646 IN denotes after
T11845 49647-49648 CD denotes 7
T11846 49649-49650 NNS denotes d
T11847 49651-49653 IN denotes of
T11848 49654-49656 FW denotes in
T11849 49657-49662 FW denotes vitro
T11850 49663-49678 NN denotes differentiation
T11851 49679-49681 IN denotes as
T11852 49682-49691 VBN denotes described
T11853 49692-49702 RB denotes previously
T11854 49703-49704 -LRB- denotes [
T11855 49704-49706 CD denotes 41
T11856 49706-49707 -RRB- denotes ]
T11857 49707-49708 . denotes .
T11858 49708-49828 sentence denotes Genomic DNA was digested with methylation-sensitive restriction enzymes as described and analysed by Southern blotting.
T11859 49709-49716 JJ denotes Genomic
T11860 49717-49720 NN denotes DNA
T11862 49721-49724 VBD denotes was
T11861 49725-49733 VBN denotes digested
T11863 49734-49738 IN denotes with
T11864 49739-49750 NN denotes methylation
T11866 49750-49751 HYPH denotes -
T11865 49751-49760 JJ denotes sensitive
T11868 49761-49772 NN denotes restriction
T11867 49773-49780 NNS denotes enzymes
T11869 49781-49783 IN denotes as
T11870 49784-49793 VBN denotes described
T11871 49794-49797 CC denotes and
T11872 49798-49806 VBN denotes analysed
T11873 49807-49809 IN denotes by
T11874 49810-49818 NNP denotes Southern
T11875 49819-49827 NN denotes blotting
T11876 49827-49828 . denotes .
T11877 49828-49972 sentence denotes The RIB3 and RIB4 probes (which were amplified from human DNA, but cross-react with the mouse rDNA repeat) have been described previously [11].
T11878 49829-49832 DT denotes The
T11880 49833-49837 NN denotes RIB3
T11881 49838-49841 CC denotes and
T11882 49842-49846 NN denotes RIB4
T11879 49847-49853 NNS denotes probes
T11884 49854-49855 -LRB- denotes (
T11885 49855-49860 WDT denotes which
T11887 49861-49865 VBD denotes were
T11886 49866-49875 VBN denotes amplified
T11888 49876-49880 IN denotes from
T11889 49881-49886 JJ denotes human
T11890 49887-49890 NN denotes DNA
T11891 49890-49892 , denotes ,
T11892 49892-49895 CC denotes but
T11893 49896-49907 VBP denotes cross-react
T11894 49908-49912 IN denotes with
T11895 49913-49916 DT denotes the
T11897 49917-49922 NN denotes mouse
T11898 49923-49927 NN denotes rDNA
T11896 49928-49934 NN denotes repeat
T11899 49934-49935 -RRB- denotes )
T11900 49936-49940 VBP denotes have
T11901 49941-49945 VBN denotes been
T11883 49946-49955 VBN denotes described
T11902 49956-49966 RB denotes previously
T11903 49967-49968 -LRB- denotes [
T11904 49968-49970 CD denotes 11
T11905 49970-49971 -RRB- denotes ]
T11906 49971-49972 . denotes .
T11907 49972-50069 sentence denotes Oligonucleotide probes to detect Line 1 and Sine B1 repeats have been described previously [42].
T11908 49973-49988 NN denotes Oligonucleotide
T11909 49989-49995 NNS denotes probes
T11911 49996-49998 TO denotes to
T11912 49999-50005 VB denotes detect
T11913 50006-50010 NN denotes Line
T11915 50011-50012 CD denotes 1
T11916 50013-50016 CC denotes and
T11917 50017-50021 NN denotes Sine
T11918 50022-50024 NN denotes B1
T11914 50025-50032 NNS denotes repeats
T11919 50033-50037 VBP denotes have
T11920 50038-50042 VBN denotes been
T11910 50043-50052 VBN denotes described
T11921 50053-50063 RB denotes previously
T11922 50064-50065 -LRB- denotes [
T11923 50065-50067 CD denotes 42
T11924 50067-50068 -RRB- denotes ]
T11925 50068-50069 . denotes .
T11926 50069-50134 sentence denotes The minor satellite probe was a 27-mer oligonucleotide (mCENT2).
T11927 50070-50073 DT denotes The
T11929 50074-50079 JJ denotes minor
T11930 50080-50089 NN denotes satellite
T11928 50090-50095 NN denotes probe
T11931 50096-50099 VBD denotes was
T11932 50100-50101 DT denotes a
T11934 50102-50104 CD denotes 27
T11936 50104-50105 HYPH denotes -
T11935 50105-50108 NN denotes mer
T11933 50109-50124 NN denotes oligonucleotide
T11937 50125-50126 -LRB- denotes (
T11938 50126-50132 NN denotes mCENT2
T11939 50132-50133 -RRB- denotes )
T11940 50133-50134 . denotes .
T11941 50134-50197 sentence denotes The major satellite probe was a 27-mer oligonucleotide (DG27).
T11942 50135-50138 DT denotes The
T11944 50139-50144 JJ denotes major
T11945 50145-50154 NN denotes satellite
T11943 50155-50160 NN denotes probe
T11946 50161-50164 VBD denotes was
T11947 50165-50166 DT denotes a
T11949 50167-50169 CD denotes 27
T11951 50169-50170 HYPH denotes -
T11950 50170-50173 NN denotes mer
T11948 50174-50189 NN denotes oligonucleotide
T11952 50190-50191 -LRB- denotes (
T11953 50191-50195 NN denotes DG27
T11954 50195-50196 -RRB- denotes )
T11955 50196-50197 . denotes .
T11956 50197-50374 sentence denotes The IAP probe was an ~400 bp PCR product (primers 14A and 13K) amplified from an IAP inserted into the mouse agouti gene [43] and was a gift from Peter Warnecke and Tim Bestor.
T11957 50198-50201 DT denotes The
T11959 50202-50205 NN denotes IAP
T11958 50206-50211 NN denotes probe
T11960 50212-50215 VBD denotes was
T11961 50216-50218 DT denotes an
T11963 50219-50220 SYM denotes ~
T11964 50220-50223 CD denotes 400
T11965 50224-50226 NN denotes bp
T11966 50227-50230 NN denotes PCR
T11962 50231-50238 NN denotes product
T11967 50239-50240 -LRB- denotes (
T11968 50240-50247 NNS denotes primers
T11969 50248-50251 NN denotes 14A
T11970 50252-50255 CC denotes and
T11971 50256-50259 NN denotes 13K
T11972 50259-50260 -RRB- denotes )
T11973 50261-50270 VBN denotes amplified
T11974 50271-50275 IN denotes from
T11975 50276-50278 DT denotes an
T11976 50279-50282 NN denotes IAP
T11977 50283-50291 VBN denotes inserted
T11978 50292-50296 IN denotes into
T11979 50297-50300 DT denotes the
T11981 50301-50306 NN denotes mouse
T11982 50307-50313 NN denotes agouti
T11980 50314-50318 NN denotes gene
T11983 50319-50320 -LRB- denotes [
T11984 50320-50322 CD denotes 43
T11985 50322-50323 -RRB- denotes ]
T11986 50324-50327 CC denotes and
T11987 50328-50331 VBD denotes was
T11988 50332-50333 DT denotes a
T11989 50334-50338 NN denotes gift
T11990 50339-50343 IN denotes from
T11991 50344-50349 NNP denotes Peter
T11992 50350-50358 NNP denotes Warnecke
T11993 50359-50362 CC denotes and
T11994 50363-50366 NNP denotes Tim
T11995 50367-50373 NNP denotes Bestor
T11996 50373-50374 . denotes .
T11997 50374-50429 sentence denotes The PCR product included the entire 5′ LTR of the IAP.
T11998 50375-50378 DT denotes The
T12000 50379-50382 NN denotes PCR
T11999 50383-50390 NN denotes product
T12001 50391-50399 VBD denotes included
T12002 50400-50403 DT denotes the
T12004 50404-50410 JJ denotes entire
T12005 50411-50412 CD denotes 5
T12006 50412-50413 SYM denotes ′
T12003 50414-50417 NN denotes LTR
T12007 50418-50420 IN denotes of
T12008 50421-50424 DT denotes the
T12009 50425-50428 NN denotes IAP
T12010 50428-50429 . denotes .
T12011 50429-50482 sentence denotes All oligonucleotide sequences are shown in Table S1.
T12012 50430-50433 DT denotes All
T12014 50434-50449 NN denotes oligonucleotide
T12013 50450-50459 NNS denotes sequences
T12016 50460-50463 VBP denotes are
T12015 50464-50469 VBN denotes shown
T12017 50470-50472 IN denotes in
T12018 50473-50478 NN denotes Table
T12019 50479-50481 NN denotes S1
T12020 50481-50482 . denotes .
T12080 50484-50494 NN denotes Generation
T12081 50495-50497 IN denotes of
T12082 50498-50506 NNS denotes chimeras
T12083 50506-50508 , denotes ,
T12084 50508-50514 VBN denotes floxed
T12085 50515-50519 NNS denotes mice
T12086 50519-50521 , denotes ,
T12087 50521-50524 CC denotes and
T12088 50525-50531 NN denotes mutant
T12089 50532-50536 NNS denotes mice
T12090 50536-50537 . denotes .
T12091 50537-50689 sentence denotes Targeted Atrx flox ES cell clones were injected into C57BL/6 blastocysts and transferred into 2.5 dpc pseudopregnant recipients by standard techniques.
T12092 50538-50546 VBN denotes Targeted
T12094 50547-50556 NN denotes Atrx flox
T12096 50557-50559 NN denotes ES
T12095 50560-50564 NN denotes cell
T12093 50565-50571 NNS denotes clones
T12098 50572-50576 VBD denotes were
T12097 50577-50585 VBN denotes injected
T12099 50586-50590 IN denotes into
T12100 50591-50596 NN denotes C57BL
T12102 50596-50597 HYPH denotes /
T12103 50597-50598 CD denotes 6
T12101 50599-50610 NNS denotes blastocysts
T12104 50611-50614 CC denotes and
T12105 50615-50626 VBN denotes transferred
T12106 50627-50631 IN denotes into
T12107 50632-50635 CD denotes 2.5
T12108 50636-50639 NN denotes dpc
T12110 50640-50654 JJ denotes pseudopregnant
T12109 50655-50665 NNS denotes recipients
T12111 50666-50668 IN denotes by
T12112 50669-50677 JJ denotes standard
T12113 50678-50688 NNS denotes techniques
T12114 50688-50689 . denotes .
T12115 50689-50768 sentence denotes Resulting chimeras were mated with C57BL/6 to establish germline transmission.
T12116 50690-50699 VBG denotes Resulting
T12117 50700-50708 NNS denotes chimeras
T12119 50709-50713 VBD denotes were
T12118 50714-50719 VBN denotes mated
T12120 50720-50724 IN denotes with
T12121 50725-50730 NN denotes C57BL
T12122 50730-50731 HYPH denotes /
T12123 50731-50732 CD denotes 6
T12124 50733-50735 TO denotes to
T12125 50736-50745 VB denotes establish
T12126 50746-50754 NN denotes germline
T12127 50755-50767 NN denotes transmission
T12128 50767-50768 . denotes .
T12129 50768-50923 sentence denotes Offspring with the Atrx flox allele were identified by Southern blotting of SacI-digested tail DNA using the intron 17 probe as shown in Figure 2A and 2C.
T12130 50769-50778 NN denotes Offspring
T12132 50779-50783 IN denotes with
T12133 50784-50787 DT denotes the
T12135 50788-50797 NN denotes Atrx flox
T12134 50798-50804 NN denotes allele
T12136 50805-50809 VBD denotes were
T12131 50810-50820 VBN denotes identified
T12137 50821-50823 IN denotes by
T12138 50824-50832 NNP denotes Southern
T12139 50833-50841 NN denotes blotting
T12140 50842-50844 IN denotes of
T12141 50845-50849 NN denotes SacI
T12143 50849-50850 HYPH denotes -
T12142 50850-50858 VBN denotes digested
T12145 50859-50863 NN denotes tail
T12144 50864-50867 NN denotes DNA
T12146 50868-50873 VBG denotes using
T12147 50874-50877 DT denotes the
T12149 50878-50884 NN denotes intron
T12150 50885-50887 CD denotes 17
T12148 50888-50893 NN denotes probe
T12151 50894-50896 IN denotes as
T12152 50897-50902 VBN denotes shown
T12153 50903-50905 IN denotes in
T12154 50906-50912 NN denotes Figure
T12155 50913-50915 NN denotes 2A
T12156 50916-50919 CC denotes and
T12157 50920-50922 NN denotes 2C
T12158 50922-50923 . denotes .
T12159 50923-51031 sentence denotes For Cre-recombination, Atrx flox mice were crossed with GATA1-Cre transgenic mice as described in the text.
T12160 50924-50927 IN denotes For
T12162 50928-50931 NN denotes Cre
T12164 50931-50932 HYPH denotes -
T12163 50932-50945 NN denotes recombination
T12165 50945-50947 , denotes ,
T12166 50947-50956 NN denotes Atrx flox
T12167 50957-50961 NNS denotes mice
T12168 50962-50966 VBD denotes were
T12161 50967-50974 VBN denotes crossed
T12169 50975-50979 IN denotes with
T12170 50980-50985 NN denotes GATA1
T12172 50985-50986 HYPH denotes -
T12171 50986-50989 NN denotes Cre
T12173 50990-51000 JJ denotes transgenic
T12174 51001-51005 NNS denotes mice
T12175 51006-51008 IN denotes as
T12176 51009-51018 VBN denotes described
T12177 51019-51021 IN denotes in
T12178 51022-51025 DT denotes the
T12179 51026-51030 NN denotes text
T12180 51030-51031 . denotes .
T12181 51031-51144 sentence denotes Recombinant alleles were detected by Southern blotting as described above or by PCR as described in Protocol S1.
T12182 51032-51043 JJ denotes Recombinant
T12183 51044-51051 NNS denotes alleles
T12185 51052-51056 VBD denotes were
T12184 51057-51065 VBN denotes detected
T12186 51066-51068 IN denotes by
T12187 51069-51077 NNP denotes Southern
T12188 51078-51086 NN denotes blotting
T12189 51087-51089 IN denotes as
T12190 51090-51099 VBN denotes described
T12191 51100-51105 RB denotes above
T12192 51106-51108 CC denotes or
T12193 51109-51111 IN denotes by
T12194 51112-51115 NN denotes PCR
T12195 51116-51118 IN denotes as
T12196 51119-51128 VBN denotes described
T12197 51129-51131 IN denotes in
T12198 51132-51140 NN denotes Protocol
T12199 51141-51143 NN denotes S1
T12200 51143-51144 . denotes .
T12292 51146-51166 NN denotes Immunohistochemistry
T12293 51166-51168 , denotes ,
T12294 51168-51170 FW denotes in
T12295 51171-51175 FW denotes situ
T12296 51176-51189 NN denotes hybridisation
T12297 51189-51191 , denotes ,
T12298 51191-51194 CC denotes and
T12299 51195-51200 NN denotes TUNEL
T12300 51201-51206 NN denotes assay
T12301 51206-51207 . denotes .
T12302 51207-51331 sentence denotes 7.5 dpc decidual swellings were dissected away from maternal tissue and fixed in 4% paraformaldehyde/PBS overnight at 4 °C.
T12303 51208-51211 CD denotes 7.5
T12304 51212-51215 NN denotes dpc
T12306 51216-51224 JJ denotes decidual
T12305 51225-51234 NNS denotes swellings
T12308 51235-51239 VBD denotes were
T12307 51240-51249 VBN denotes dissected
T12309 51250-51254 RB denotes away
T12310 51255-51259 IN denotes from
T12311 51260-51268 JJ denotes maternal
T12312 51269-51275 NN denotes tissue
T12313 51276-51279 CC denotes and
T12314 51280-51285 VBN denotes fixed
T12315 51286-51288 IN denotes in
T12316 51289-51290 CD denotes 4
T12317 51290-51291 NN denotes %
T12318 51292-51308 NN denotes paraformaldehyde
T12319 51308-51309 HYPH denotes /
T12320 51309-51312 NN denotes PBS
T12321 51313-51322 RB denotes overnight
T12322 51323-51325 IN denotes at
T12323 51326-51327 CD denotes 4
T12324 51328-51330 NN denotes °C
T12325 51330-51331 . denotes .
T12326 51331-51480 sentence denotes After embryos were washed thoroughly in PBS, they were dehydrated through an ethanol series and xylene, embedded in paraffin, and sectioned at 5 μm.
T12327 51332-51337 IN denotes After
T12329 51338-51345 NNS denotes embryos
T12330 51346-51350 VBD denotes were
T12328 51351-51357 VBN denotes washed
T12332 51358-51368 RB denotes thoroughly
T12333 51369-51371 IN denotes in
T12334 51372-51375 NN denotes PBS
T12335 51375-51377 , denotes ,
T12336 51377-51381 PRP denotes they
T12337 51382-51386 VBD denotes were
T12331 51387-51397 VBN denotes dehydrated
T12338 51398-51405 IN denotes through
T12339 51406-51408 DT denotes an
T12341 51409-51416 NN denotes ethanol
T12340 51417-51423 NNS denotes series
T12342 51424-51427 CC denotes and
T12343 51428-51434 NN denotes xylene
T12344 51434-51436 , denotes ,
T12345 51436-51444 VBN denotes embedded
T12346 51445-51447 IN denotes in
T12347 51448-51456 NN denotes paraffin
T12348 51456-51458 , denotes ,
T12349 51458-51461 CC denotes and
T12350 51462-51471 VBN denotes sectioned
T12351 51472-51474 IN denotes at
T12352 51475-51476 CD denotes 5
T12353 51477-51479 NNS denotes μm
T12354 51479-51480 . denotes .
T12355 51480-51671 sentence denotes Sections were processed for immunohistochemistry using the ABC Staining System (Santa Cruz Biotechnology, Santa Cruz, California, United States) according to the manufacturer's instructions.
T12356 51481-51489 NNS denotes Sections
T12358 51490-51494 VBD denotes were
T12357 51495-51504 VBN denotes processed
T12359 51505-51508 IN denotes for
T12360 51509-51529 NN denotes immunohistochemistry
T12361 51530-51535 VBG denotes using
T12362 51536-51539 DT denotes the
T12364 51540-51543 NNP denotes ABC
T12365 51544-51552 NNP denotes Staining
T12363 51553-51559 NNP denotes System
T12366 51560-51561 -LRB- denotes (
T12368 51561-51566 NNP denotes Santa
T12369 51567-51571 NNP denotes Cruz
T12367 51572-51585 NNP denotes Biotechnology
T12370 51585-51587 , denotes ,
T12371 51587-51592 NNP denotes Santa
T12372 51593-51597 NNP denotes Cruz
T12373 51597-51599 , denotes ,
T12374 51599-51609 NNP denotes California
T12375 51609-51611 , denotes ,
T12376 51611-51617 NNP denotes United
T12377 51618-51624 NNP denotes States
T12378 51624-51625 -RRB- denotes )
T12379 51626-51635 VBG denotes according
T12380 51636-51638 IN denotes to
T12381 51639-51642 DT denotes the
T12382 51643-51655 NN denotes manufacturer
T12384 51655-51657 POS denotes 's
T12383 51658-51670 NNS denotes instructions
T12385 51670-51671 . denotes .
T12386 51671-51969 sentence denotes Sections were stained with rabbit polyclonal antibodies against ATRX (H-300, Santa Cruz Biotechnology), Placental lactogen-I (AB1288, Chemicon International, Temecula, California, United States) and phospho (Ser10)-histone H3 (06–570, Upstate Biotechnology, Waltham, Massachusetts, United States).
T12387 51672-51680 NNS denotes Sections
T12389 51681-51685 VBD denotes were
T12388 51686-51693 VBN denotes stained
T12390 51694-51698 IN denotes with
T12391 51699-51705 NN denotes rabbit
T12393 51706-51716 JJ denotes polyclonal
T12392 51717-51727 NNS denotes antibodies
T12394 51728-51735 IN denotes against
T12395 51736-51740 NN denotes ATRX
T12396 51741-51742 -LRB- denotes (
T12397 51742-51743 NN denotes H
T12398 51743-51744 HYPH denotes -
T12399 51744-51747 CD denotes 300
T12400 51747-51749 , denotes ,
T12401 51749-51754 NNP denotes Santa
T12402 51755-51759 NNP denotes Cruz
T12403 51760-51773 NNP denotes Biotechnology
T12404 51773-51774 -RRB- denotes )
T12405 51774-51776 , denotes ,
T12406 51776-51785 JJ denotes Placental
T12408 51786-51794 NN denotes lactogen
T12409 51794-51795 HYPH denotes -
T12407 51795-51796 NN denotes I
T12410 51797-51798 -LRB- denotes (
T12411 51798-51804 NN denotes AB1288
T12412 51804-51806 , denotes ,
T12413 51806-51814 NNP denotes Chemicon
T12414 51815-51828 NNP denotes International
T12415 51828-51830 , denotes ,
T12416 51830-51838 NNP denotes Temecula
T12417 51838-51840 , denotes ,
T12418 51840-51850 NNP denotes California
T12419 51850-51852 , denotes ,
T12420 51852-51858 NNP denotes United
T12421 51859-51865 NNP denotes States
T12422 51865-51866 -RRB- denotes )
T12423 51867-51870 CC denotes and
T12424 51871-51878 NN denotes phospho
T12426 51879-51880 -LRB- denotes (
T12427 51880-51885 NN denotes Ser10
T12428 51885-51886 -RRB- denotes )
T12429 51886-51887 HYPH denotes -
T12425 51887-51894 NN denotes histone
T12430 51895-51897 NN denotes H3
T12431 51898-51899 -LRB- denotes (
T12433 51899-51901 CD denotes 06
T12434 51901-51902 HYPH denotes –
T12432 51902-51905 CD denotes 570
T12435 51905-51907 , denotes ,
T12436 51907-51914 NNP denotes Upstate
T12437 51915-51928 NNP denotes Biotechnology
T12438 51928-51930 , denotes ,
T12439 51930-51937 NNP denotes Waltham
T12440 51937-51939 , denotes ,
T12441 51939-51952 NNP denotes Massachusetts
T12442 51952-51954 , denotes ,
T12443 51954-51960 NNP denotes United
T12444 51961-51967 NNP denotes States
T12445 51967-51968 -RRB- denotes )
T12446 51968-51969 . denotes .
T12447 51969-52038 sentence denotes Where appropriate, adjacent sections were stained with haematoxylin.
T12448 51970-51975 WRB denotes Where
T12449 51976-51987 JJ denotes appropriate
T12451 51987-51989 , denotes ,
T12452 51989-51997 JJ denotes adjacent
T12453 51998-52006 NNS denotes sections
T12454 52007-52011 VBD denotes were
T12450 52012-52019 VBN denotes stained
T12455 52020-52024 IN denotes with
T12456 52025-52037 NN denotes haematoxylin
T12457 52037-52038 . denotes .
T12458 52038-52197 sentence denotes In some cases, adjacent sections were also analysed to detect apoptotic cells by TUNEL using the in situ cell death detection kit (Roche, Basel, Switzerland).
T12459 52039-52041 IN denotes In
T12461 52042-52046 DT denotes some
T12462 52047-52052 NNS denotes cases
T12463 52052-52054 , denotes ,
T12464 52054-52062 JJ denotes adjacent
T12465 52063-52071 NNS denotes sections
T12466 52072-52076 VBD denotes were
T12467 52077-52081 RB denotes also
T12460 52082-52090 VBN denotes analysed
T12468 52091-52093 TO denotes to
T12469 52094-52100 VB denotes detect
T12470 52101-52110 JJ denotes apoptotic
T12471 52111-52116 NNS denotes cells
T12472 52117-52119 IN denotes by
T12473 52120-52125 NN denotes TUNEL
T12474 52126-52131 VBG denotes using
T12475 52132-52135 DT denotes the
T12477 52136-52138 FW denotes in
T12478 52139-52143 FW denotes situ
T12479 52144-52148 NN denotes cell
T12480 52149-52154 NN denotes death
T12481 52155-52164 NN denotes detection
T12476 52165-52168 NN denotes kit
T12482 52169-52170 -LRB- denotes (
T12483 52170-52175 NNP denotes Roche
T12484 52175-52177 , denotes ,
T12485 52177-52182 NNP denotes Basel
T12486 52182-52184 , denotes ,
T12487 52184-52195 NNP denotes Switzerland
T12488 52195-52196 -RRB- denotes )
T12489 52196-52197 . denotes .
T12490 52197-52375 sentence denotes After labelling, these slides were mounted in Vectashield containing DAPI (Vector Laboratories, Burlingame, California, United States) and visualised by fluorescence microscopy.
T12491 52198-52203 IN denotes After
T12493 52204-52213 NN denotes labelling
T12494 52213-52215 , denotes ,
T12495 52215-52220 DT denotes these
T12496 52221-52227 NNS denotes slides
T12497 52228-52232 VBD denotes were
T12492 52233-52240 VBN denotes mounted
T12498 52241-52243 IN denotes in
T12499 52244-52255 NNP denotes Vectashield
T12500 52256-52266 VBG denotes containing
T12501 52267-52271 NN denotes DAPI
T12502 52272-52273 -LRB- denotes (
T12504 52273-52279 NNP denotes Vector
T12503 52280-52292 NNP denotes Laboratories
T12505 52292-52294 , denotes ,
T12506 52294-52304 NNP denotes Burlingame
T12507 52304-52306 , denotes ,
T12508 52306-52316 NNP denotes California
T12509 52316-52318 , denotes ,
T12510 52318-52324 NNP denotes United
T12511 52325-52331 NNP denotes States
T12512 52331-52332 -RRB- denotes )
T12513 52333-52336 CC denotes and
T12514 52337-52347 VBN denotes visualised
T12515 52348-52350 IN denotes by
T12516 52351-52363 NN denotes fluorescence
T12517 52364-52374 NN denotes microscopy
T12518 52374-52375 . denotes .
T12519 52375-52706 sentence denotes Whole-mount in situ hybridisations were performed on 8.5 dpc embryos (dissected away from maternal and extraembryonic tissues) using a brachyury (T) riboprobe [16] and on bisected decidual implantation sites from which embryos (8.5 dpc) had been removed using a placental lactogen-1 (Pl-1) riboprobe (see Protocol S1 for details).
T12520 52376-52381 JJ denotes Whole
T12522 52381-52382 HYPH denotes -
T12521 52382-52387 NN denotes mount
T12524 52388-52390 FW denotes in
T12525 52391-52395 FW denotes situ
T12523 52396-52410 NNS denotes hybridisations
T12527 52411-52415 VBD denotes were
T12526 52416-52425 VBN denotes performed
T12528 52426-52428 IN denotes on
T12529 52429-52432 CD denotes 8.5
T12530 52433-52436 NN denotes dpc
T12531 52437-52444 NNS denotes embryos
T12532 52445-52446 -LRB- denotes (
T12533 52446-52455 VBN denotes dissected
T12534 52456-52460 RB denotes away
T12535 52461-52465 IN denotes from
T12536 52466-52474 JJ denotes maternal
T12538 52475-52478 CC denotes and
T12539 52479-52493 JJ denotes extraembryonic
T12537 52494-52501 NNS denotes tissues
T12540 52501-52502 -RRB- denotes )
T12541 52503-52508 VBG denotes using
T12542 52509-52510 DT denotes a
T12544 52511-52520 NN denotes brachyury
T12545 52521-52522 -LRB- denotes (
T12546 52522-52523 NN denotes T
T12547 52523-52524 -RRB- denotes )
T12543 52525-52534 NN denotes riboprobe
T12548 52535-52536 -LRB- denotes [
T12549 52536-52538 CD denotes 16
T12550 52538-52539 -RRB- denotes ]
T12551 52540-52543 CC denotes and
T12552 52544-52546 IN denotes on
T12553 52547-52555 VBN denotes bisected
T12555 52556-52564 JJ denotes decidual
T12556 52565-52577 NN denotes implantation
T12554 52578-52583 NNS denotes sites
T12557 52584-52588 IN denotes from
T12559 52589-52594 WDT denotes which
T12560 52595-52602 NNS denotes embryos
T12561 52603-52604 -LRB- denotes (
T12563 52604-52607 CD denotes 8.5
T12562 52608-52611 NN denotes dpc
T12564 52611-52612 -RRB- denotes )
T12565 52613-52616 VBD denotes had
T12566 52617-52621 VBN denotes been
T12558 52622-52629 VBN denotes removed
T12567 52630-52635 VBG denotes using
T12568 52636-52637 DT denotes a
T12570 52638-52647 JJ denotes placental
T12571 52648-52656 NN denotes lactogen
T12572 52656-52657 HYPH denotes -
T12573 52657-52658 CD denotes 1
T12574 52659-52660 -LRB- denotes (
T12575 52660-52662 NN denotes Pl
T12576 52662-52663 HYPH denotes -
T12577 52663-52664 CD denotes 1
T12578 52664-52665 -RRB- denotes )
T12569 52666-52675 NN denotes riboprobe
T12579 52676-52677 -LRB- denotes (
T12580 52677-52680 VB denotes see
T12581 52681-52689 NN denotes Protocol
T12582 52690-52692 NN denotes S1
T12583 52693-52696 IN denotes for
T12584 52697-52704 NNS denotes details
T12585 52704-52705 -RRB- denotes )
T12586 52705-52706 . denotes .
T12623 52708-52718 NN denotes Blastocyst
T12624 52719-52728 NN denotes outgrowth
T12625 52729-52737 NNS denotes cultures
T12626 52737-52738 . denotes .
T12627 52738-52932 sentence denotes Superovulated heterozygous female mice (Atrx WT/flox) were mated to homozygous GATA1-cre +/+ transgenic males, and blastocysts were flushed from uterine horns with M2 medium (Sigma) at 3.5 dpc.
T12628 52739-52752 VBN denotes Superovulated
T12630 52753-52765 JJ denotes heterozygous
T12631 52766-52772 JJ denotes female
T12629 52773-52777 NNS denotes mice
T12633 52778-52779 -LRB- denotes (
T12635 52779-52786 NN denotes Atrx WT
T12636 52786-52787 HYPH denotes /
T12634 52787-52791 NN denotes flox
T12637 52791-52792 -RRB- denotes )
T12638 52793-52797 VBD denotes were
T12632 52798-52803 VBN denotes mated
T12639 52804-52806 IN denotes to
T12640 52807-52817 JJ denotes homozygous
T12642 52818-52823 NN denotes GATA1
T12644 52823-52824 HYPH denotes -
T12643 52824-52827 NN denotes cre
T12645 52828-52829 SYM denotes +
T12646 52829-52830 HYPH denotes /
T12647 52830-52831 SYM denotes +
T12648 52832-52842 JJ denotes transgenic
T12641 52843-52848 NNS denotes males
T12649 52848-52850 , denotes ,
T12650 52850-52853 CC denotes and
T12651 52854-52865 NNS denotes blastocysts
T12653 52866-52870 VBD denotes were
T12652 52871-52878 VBN denotes flushed
T12654 52879-52883 IN denotes from
T12655 52884-52891 JJ denotes uterine
T12656 52892-52897 NNS denotes horns
T12657 52898-52902 IN denotes with
T12658 52903-52905 NN denotes M2
T12659 52906-52912 NN denotes medium
T12660 52913-52914 -LRB- denotes (
T12661 52914-52919 NNP denotes Sigma
T12662 52919-52920 -RRB- denotes )
T12663 52921-52923 IN denotes at
T12664 52924-52927 CD denotes 3.5
T12665 52928-52931 NNS denotes dpc
T12666 52931-52932 . denotes .
T12667 52932-53035 sentence denotes Individual blastocysts were cultured in multiwell tissue cultures plates as described previously [44].
T12668 52933-52943 JJ denotes Individual
T12669 52944-52955 NNS denotes blastocysts
T12671 52956-52960 VBD denotes were
T12670 52961-52969 VBN denotes cultured
T12672 52970-52972 IN denotes in
T12673 52973-52982 JJ denotes multiwell
T12675 52983-52989 NN denotes tissue
T12674 52990-52998 NNS denotes cultures
T12676 52999-53005 NNS denotes plates
T12677 53006-53008 IN denotes as
T12678 53009-53018 VBN denotes described
T12679 53019-53029 RB denotes previously
T12680 53030-53031 -LRB- denotes [
T12681 53031-53033 CD denotes 44
T12682 53033-53034 -RRB- denotes ]
T12683 53034-53035 . denotes .
T12684 53035-53118 sentence denotes Cultures were inspected and photographed daily and the extent of outgrowth scored.
T12685 53036-53044 NNS denotes Cultures
T12687 53045-53049 VBD denotes were
T12686 53050-53059 VBN denotes inspected
T12688 53060-53063 CC denotes and
T12689 53064-53076 VBN denotes photographed
T12690 53077-53082 RB denotes daily
T12691 53083-53086 CC denotes and
T12692 53087-53090 DT denotes the
T12693 53091-53097 NN denotes extent
T12695 53098-53100 IN denotes of
T12696 53101-53110 NN denotes outgrowth
T12694 53111-53117 VBN denotes scored
T12697 53117-53118 . denotes .
T12698 53118-53172 sentence denotes After 7 d, cultures were harvested and DNA extracted.
T12699 53119-53124 IN denotes After
T12701 53125-53126 CD denotes 7
T12702 53127-53128 NNS denotes d
T12703 53128-53130 , denotes ,
T12704 53130-53138 NNS denotes cultures
T12705 53139-53143 VBD denotes were
T12700 53144-53153 VBN denotes harvested
T12706 53154-53157 CC denotes and
T12707 53158-53161 NN denotes DNA
T12708 53162-53171 VBN denotes extracted
T12709 53171-53172 . denotes .
T12710 53172-53265 sentence denotes The Atrx genotype and sex of each culture was determined by PCR as described in Protocol S1.
T12711 53173-53176 DT denotes The
T12713 53177-53181 NN denotes Atrx
T12712 53182-53190 NN denotes genotype
T12715 53191-53194 CC denotes and
T12716 53195-53198 NN denotes sex
T12717 53199-53201 IN denotes of
T12718 53202-53206 DT denotes each
T12719 53207-53214 NN denotes culture
T12720 53215-53218 VBD denotes was
T12714 53219-53229 VBN denotes determined
T12721 53230-53232 IN denotes by
T12722 53233-53236 NN denotes PCR
T12723 53237-53239 IN denotes as
T12724 53240-53249 VBN denotes described
T12725 53250-53252 IN denotes in
T12726 53253-53261 NN denotes Protocol
T12727 53262-53264 NN denotes S1
T12728 53264-53265 . denotes .
R1000 T1755 T1753 dobj clones,recovered
R1001 T1756 T1757 prep in,flanked
R1002 T1757 T1755 relcl flanked,clones
R1003 T1758 T1756 pobj which,in
R1004 T1759 T1757 nsubjpass exon,flanked
R1005 T1760 T1759 nummod 18,exon
R1006 T1761 T1757 aux has,flanked
R1007 T1762 T1757 auxpass been,flanked
R1008 T1763 T1757 agent by,flanked
R1009 T1764 T1765 compound loxP,sites
R1010 T1765 T1763 pobj sites,by
R1011 T1766 T1765 compound recognition,sites
R1012 T1767 T1765 prep for,sites
R1013 T1768 T1769 det the,recombinase
R1014 T1769 T1767 pobj recombinase,for
R1015 T1770 T1769 compound Cre,recombinase
R1016 T1771 T1772 punct (,allele
R1017 T1772 T1769 parataxis allele,recombinase
R1018 T1773 T1772 compound Atrx flox,allele
R1019 T1774 T1772 prep in,allele
R1020 T1775 T1776 compound Figure,2A
R1021 T1776 T1774 pobj 2A,in
R1022 T1777 T1772 punct ),allele
R1023 T1778 T1779 punct (,2B
R1024 T1779 T1753 parataxis 2B,recovered
R1025 T1780 T1779 compound Figure,2B
R1026 T1781 T1779 punct ),2B
R1027 T1782 T1739 punct .,adopted
R1028 T1784 T1785 det This,allele
R1029 T1785 T1786 nsubj allele,contains
R1030 T1787 T1786 advmod also,contains
R1031 T1788 T1789 det a,cassette
R1032 T1789 T1786 dobj cassette,contains
R1033 T1790 T1791 npadvmod loxP,flanked
R1034 T1791 T1789 amod flanked,cassette
R1035 T1792 T1791 punct -,flanked
R1036 T1793 T1794 compound MC1,neor
R1037 T1794 T1789 compound neor,cassette
R1038 T1795 T1794 punct -,neor
R1039 T1796 T1786 prep in,contains
R1040 T1797 T1796 pobj intron,in
R1041 T1798 T1797 nummod 17,intron
R1042 T1799 T1800 punct (,2A
R1043 T1800 T1786 parataxis 2A,contains
R1044 T1801 T1800 compound Figure,2A
R1045 T1802 T1800 punct ),2A
R1046 T1803 T1786 punct .,contains
R1047 T1805 T1806 nmod Northern,blot
R1048 T1806 T1809 compound blot,analyses
R1049 T1807 T1805 cc and,Northern
R1050 T1808 T1805 conj Western,Northern
R1051 T1809 T1810 nsubj analyses,confirmed
R1052 T1811 T1812 punct (,2D
R1053 T1812 T1809 parataxis 2D,analyses
R1054 T1813 T1812 compound Figure,2D
R1055 T1814 T1812 cc and,2D
R1056 T1815 T1812 conj 2E,2D
R1057 T1816 T1812 punct ),2D
R1058 T1817 T1818 mark that,continued
R1059 T1818 T1810 ccomp continued,confirmed
R1060 T1819 T1820 det the,clones
R1061 T1820 T1818 nsubj clones,continued
R1062 T1821 T1820 compound Atrx flox,clones
R1063 T1822 T1823 aux to,express
R1064 T1823 T1818 xcomp express,continued
R1065 T1824 T1825 preconj both,protein
R1066 T1825 T1823 dobj protein,express
R1067 T1826 T1827 amod full,length
R1068 T1827 T1825 compound length,protein
R1069 T1828 T1827 punct -,length
R1070 T1829 T1825 compound Atrx,protein
R1071 T1830 T1825 cc and,protein
R1072 T1831 T1832 det the,isoform
R1073 T1832 T1825 conj isoform,protein
R1074 T1833 T1832 amod truncated,isoform
R1075 T1834 T1832 compound Atrxt,isoform
R1076 T1835 T1810 punct .,confirmed
R1077 T1837 T1838 aux To,generate
R1078 T1838 T1839 advcl generate,transfected
R1079 T1840 T1841 det the,deletion
R1080 T1841 T1838 dobj deletion,generate
R1081 T1842 T1841 amod full,deletion
R1082 T1843 T1838 prep in,generate
R1083 T1844 T1845 compound ES,cells
R1084 T1845 T1843 pobj cells,in
R1085 T1846 T1839 punct ", ",transfected
R1086 T1847 T1848 det the,clones
R1087 T1848 T1839 nsubjpass clones,transfected
R1088 T1849 T1848 compound Atrx flox,clones
R1089 T1850 T1851 punct (,F12
R1090 T1851 T1848 parataxis F12,clones
R1091 T1852 T1851 nummod 1,F12
R1092 T1853 T1851 punct /,F12
R1093 T1854 T1851 cc and,F12
R1094 T1855 T1856 nummod 1,G11
R1095 T1856 T1851 conj G11,F12
R1096 T1857 T1856 punct /,G11
R1097 T1858 T1851 punct ),F12
R1098 T1859 T1839 auxpass were,transfected
R1099 T1860 T1839 advmod transiently,transfected
R1100 T1861 T1839 prep with,transfected
R1101 T1862 T1863 det a,plasmid
R1102 T1863 T1861 pobj plasmid,with
R1103 T1864 T1865 compound Cre,recombinase
R1104 T1865 T1863 compound recombinase,plasmid
R1105 T1866 T1865 punct -,recombinase
R1106 T1867 T1863 compound expression,plasmid
R1107 T1868 T1863 punct (,plasmid
R1108 T1869 T1870 compound pCAGGS,IRESpuro
R1109 T1870 T1863 appos IRESpuro,plasmid
R1110 T1871 T1870 punct -,IRESpuro
R1111 T1872 T1870 compound Cre,IRESpuro
R1112 T1873 T1870 punct -,IRESpuro
R1113 T1874 T1839 punct ),transfected
R1114 T1875 T1839 punct ", ",transfected
R1115 T1876 T1839 cc and,transfected
R1116 T1877 T1878 nsubjpass subclones,bearing
R1117 T1878 T1839 conj bearing,transfected
R1118 T1879 T1878 auxpass were,bearing
R1119 T1880 T1878 aux recovered,bearing
R1120 T1881 T1882 det an,allele
R1121 T1882 T1878 dobj allele,bearing
R1122 T1883 T1884 punct (,Atrx Δ18Δneo
R1123 T1884 T1882 parataxis Atrx Δ18Δneo,allele
R1124 T1885 T1884 prep in,Atrx Δ18Δneo
R1125 T1886 T1887 compound Figure,2A
R1126 T1887 T1885 pobj 2A,in
R1127 T1888 T1884 punct ),Atrx Δ18Δneo
R1128 T1889 T1890 prep in,deleted
R1129 T1890 T1882 relcl deleted,allele
R1130 T1891 T1889 pobj which,in
R1131 T1892 T1893 preconj both,exon
R1132 T1893 T1890 nsubjpass exon,deleted
R1133 T1894 T1893 nummod 18,exon
R1134 T1895 T1893 cc and,exon
R1135 T1896 T1897 det the,cassette
R1136 T1897 T1893 conj cassette,exon
R1137 T1898 T1897 compound neor,cassette
R1138 T1899 T1890 aux had,deleted
R1139 T1900 T1890 auxpass been,deleted
R1140 T1901 T1890 agent by,deleted
R1141 T1902 T1903 det the,recombinase
R1142 T1903 T1901 pobj recombinase,by
R1143 T1904 T1903 compound Cre,recombinase
R1144 T1905 T1906 punct (,resulting
R1145 T1906 T1890 parataxis resulting,deleted
R1146 T1907 T1906 prep from,resulting
R1147 T1908 T1909 det the,event
R1148 T1909 T1907 pobj event,from
R1149 T1910 T1909 compound recombination,event
R1150 T1911 T1909 acl labelled,event
R1151 T1912 T1913 punct “,C
R1152 T1913 T1911 oprd C,labelled
R1153 T1914 T1911 punct ”,labelled
R1154 T1915 T1911 prep in,labelled
R1155 T1916 T1917 det the,allele
R1156 T1917 T1915 pobj allele,in
R1157 T1918 T1917 compound Atrx flox,allele
R1158 T1919 T1917 acl shown,allele
R1159 T1920 T1919 prep in,shown
R1160 T1921 T1922 compound Figure,2A
R1161 T1922 T1920 pobj 2A,in
R1162 T1923 T1906 punct ),resulting
R1163 T1924 T1925 punct (,2C
R1164 T1925 T1890 parataxis 2C,deleted
R1165 T1926 T1925 compound Figure,2C
R1166 T1927 T1925 punct ),2C
R1167 T1928 T1878 punct .,bearing
R1168 T1930 T1931 nmod Northern,blot
R1169 T1931 T1934 compound blot,analyses
R1170 T1932 T1930 cc and,Northern
R1171 T1933 T1930 conj Western,Northern
R1172 T1934 T1935 nsubj analyses,revealed
R1173 T1936 T1937 punct (,2D
R1174 T1937 T1934 parataxis 2D,analyses
R1175 T1938 T1937 compound Figure,2D
R1176 T1939 T1937 cc and,2D
R1177 T1940 T1937 conj 2E,2D
R1178 T1941 T1937 punct ),2D
R1179 T1942 T1943 mark that,abolished
R1180 T1943 T1935 ccomp abolished,revealed
R1181 T1944 T1945 det the,transcript
R1182 T1945 T1943 nsubjpass transcript,abolished
R1183 T1946 T1947 amod full,length
R1184 T1947 T1945 compound length,transcript
R1185 T1948 T1947 punct -,length
R1186 T1949 T1945 compound Atrx,transcript
R1187 T1950 T1945 cc and,transcript
R1188 T1951 T1945 conj protein,transcript
R1189 T1952 T1943 auxpass is,abolished
R1190 T1953 T1943 advmod completely,abolished
R1191 T1954 T1943 prep in,abolished
R1192 T1955 T1956 det the,clones
R1193 T1956 T1954 pobj clones,in
R1194 T1957 T1958 npadvmod Atrx Δ18Δneo,recombinant
R1195 T1958 T1956 amod recombinant,clones
R1196 T1959 T1943 punct ", ",abolished
R1197 T1960 T1943 advcl suggesting,abolished
R1198 T1961 T1962 mark that,has
R1199 T1962 T1960 ccomp has,suggesting
R1200 T1963 T1962 nsubj deletion,has
R1201 T1964 T1963 prep of,deletion
R1202 T1965 T1966 det this,region
R1203 T1966 T1964 pobj region,of
R1204 T1967 T1968 det a,effect
R1205 T1968 T1962 dobj effect,has
R1206 T1969 T1970 advmod highly,destabilising
R1207 T1970 T1968 amod destabilising,effect
R1208 T1971 T1962 prep on,has
R1209 T1972 T1973 det the,transcript
R1210 T1973 T1971 pobj transcript,on
R1211 T1974 T1975 amod full,length
R1212 T1975 T1973 compound length,transcript
R1213 T1976 T1975 punct -,length
R1214 T1977 T1935 punct .,revealed
R1215 T1979 T1980 mark As,expected
R1216 T1980 T1981 advcl expected,is
R1217 T1982 T1981 punct ", ",is
R1218 T1983 T1984 det the,isoform
R1219 T1984 T1981 nsubj isoform,is
R1220 T1985 T1984 amod truncated,isoform
R1221 T1986 T1984 compound Atrxt,isoform
R1222 T1987 T1984 punct ", ",isoform
R1223 T1988 T1989 det the,transcript
R1224 T1989 T1990 dep transcript,terminated
R1225 T1990 T1984 relcl terminated,isoform
R1226 T1991 T1989 prep of,transcript
R1227 T1992 T1991 pobj which,of
R1228 T1993 T1990 auxpass is,terminated
R1229 T1994 T1990 prep within,terminated
R1230 T1995 T1994 pobj intron,within
R1231 T1996 T1995 nummod 11,intron
R1232 T1997 T1998 punct [,4
R1233 T1998 T1990 parataxis 4,terminated
R1234 T1999 T1998 punct ],4
R1235 T2000 T1981 punct ", ",is
R1236 T2001 T1981 acomp unaffected,is
R1237 T2002 T2001 prep by,unaffected
R1238 T2003 T2004 det the,deletion
R1239 T2004 T2002 pobj deletion,by
R1240 T2005 T2004 prep of,deletion
R1241 T2006 T2005 pobj exon,of
R1242 T2007 T2006 nummod 18,exon
R1243 T2008 T2009 punct (,2E
R1244 T2009 T1981 parataxis 2E,is
R1245 T2010 T2009 compound Figure,2E
R1246 T2011 T2009 punct ),2E
R1247 T2012 T1981 punct .,is
R1248 T2014 T2015 mark While,is
R1249 T2015 T2020 advcl is,is
R1250 T2016 T2017 det the,function
R1251 T2017 T2015 nsubj function,is
R1252 T2018 T2017 prep of,function
R1253 T2019 T2018 pobj Atrxt,of
R1254 T2021 T2015 neg not,is
R1255 T2022 T2015 advmod yet,is
R1256 T2023 T2015 acomp clear,is
R1257 T2024 T2020 punct ", ",is
R1258 T2025 T2026 det this,isoform
R1259 T2026 T2020 nsubj isoform,is
R1260 T2027 T2026 punct ", ",isoform
R1261 T2028 T2029 dep which,contains
R1262 T2029 T2026 relcl contains,isoform
R1263 T2030 T2031 det the,domain
R1264 T2031 T2029 dobj domain,contains
R1265 T2032 T2033 npadvmod PHD,like
R1266 T2033 T2031 amod like,domain
R1267 T2034 T2033 punct -,like
R1268 T2035 T2031 cc but,domain
R1269 T2036 T2035 neg not,but
R1270 T2037 T2038 det the,motifs
R1271 T2038 T2031 conj motifs,domain
R1272 T2039 T2040 compound SWI,SNF
R1273 T2040 T2038 compound SNF,motifs
R1274 T2041 T2040 punct /,SNF
R1275 T2042 T2043 punct (,Figure
R1276 T2043 T2029 parataxis Figure,contains
R1277 T2044 T2043 nummod 1,Figure
R1278 T2045 T2043 punct ),Figure
R1279 T2046 T2020 punct ", ",is
R1280 T2047 T2020 acomp unlikely,is
R1281 T2048 T2049 aux to,be
R1282 T2049 T2047 xcomp be,unlikely
R1283 T2050 T2051 advmod functionally,equivalent
R1284 T2051 T2049 acomp equivalent,be
R1285 T2052 T2051 prep to,equivalent
R1286 T2053 T2054 det the,protein
R1287 T2054 T2052 pobj protein,to
R1288 T2055 T2056 amod full,length
R1289 T2056 T2054 compound length,protein
R1290 T2057 T2056 punct -,length
R1291 T2058 T2020 punct .,is
R1292 T2060 T2061 advmod Thus,allowed
R1293 T2062 T2061 punct ", ",allowed
R1294 T2063 T2064 det a,strategy
R1295 T2064 T2061 nsubj strategy,allowed
R1296 T2065 T2066 amod conditional,knockout
R1297 T2066 T2064 compound knockout,strategy
R1298 T2067 T2068 det the,isolation
R1299 T2068 T2061 dobj isolation,allowed
R1300 T2069 T2068 prep of,isolation
R1301 T2070 T2071 compound ES,cells
R1302 T2071 T2069 pobj cells,of
R1303 T2072 T2073 dep that,are
R1304 T2073 T2068 relcl are,isolation
R1305 T2074 T2073 acomp null,are
R1306 T2075 T2074 prep for,null
R1307 T2076 T2077 amod full,length
R1308 T2077 T2079 compound length,Atrx
R1309 T2078 T2077 punct -,length
R1310 T2079 T2075 pobj Atrx,for
R1311 T2080 T2061 punct .,allowed
R1313 T2534 T2535 amod Perturbed,Growth
R1314 T2536 T2535 cc and,Growth
R1315 T2537 T2538 compound Methylation,Defects
R1316 T2538 T2535 conj Defects,Growth
R1317 T2539 T2535 prep in,Growth
R1318 T2540 T2541 amod Atrxnull,Cells
R1319 T2541 T2539 pobj Cells,in
R1320 T2542 T2541 compound ES,Cells
R1321 T2544 T2545 amod Atrxnull,cells
R1322 T2545 T2547 nsubjpass cells,maintained
R1323 T2546 T2545 compound ES,cells
R1324 T2548 T2547 aux could,maintained
R1325 T2549 T2547 auxpass be,maintained
R1326 T2550 T2547 prep in,maintained
R1327 T2551 T2550 pobj culture,in
R1328 T2552 T2547 cc but,maintained
R1329 T2553 T2547 conj were,maintained
R1330 T2554 T2553 advmod generally,were
R1331 T2555 T2556 advmod slower,growing
R1332 T2556 T2553 acomp growing,were
R1333 T2557 T2556 prep than,growing
R1334 T2558 T2559 nmod Atrx,ES
R1335 T2559 T2561 compound ES,clones
R1336 T2560 T2559 punct +,ES
R1337 T2561 T2557 pobj clones,than
R1338 T2562 T2553 punct ", ",were
R1339 T2563 T2553 cc and,were
R1340 T2564 T2553 conj appeared,were
R1341 T2565 T2566 aux to,undergo
R1342 T2566 T2564 xcomp undergo,appeared
R1343 T2567 T2568 amod higher,rates
R1344 T2568 T2566 dobj rates,undergo
R1345 T2569 T2568 prep of,rates
R1346 T2570 T2571 amod spontaneous,differentiation
R1347 T2571 T2569 pobj differentiation,of
R1348 T2572 T2547 punct .,maintained
R1349 T2574 T2575 nsubj We,investigated
R1350 T2576 T2575 advmod directly,investigated
R1351 T2577 T2578 det the,effect
R1352 T2578 T2575 dobj effect,investigated
R1353 T2579 T2578 prep of,effect
R1354 T2580 T2579 pobj Atrx,of
R1355 T2581 T2578 prep on,effect
R1356 T2582 T2583 compound ES,cell
R1357 T2583 T2584 compound cell,growth
R1358 T2584 T2581 pobj growth,on
R1359 T2585 T2575 prep by,investigated
R1360 T2586 T2585 pcomp comparing,by
R1361 T2587 T2588 nmod Atrx,clones
R1362 T2588 T2586 dobj clones,comparing
R1363 T2589 T2587 punct +,Atrx
R1364 T2590 T2587 cc and,Atrx
R1365 T2591 T2587 conj Atrxnull,Atrx
R1366 T2592 T2593 compound ES,cell
R1367 T2593 T2588 compound cell,clones
R1368 T2594 T2586 prep in,comparing
R1369 T2595 T2596 compound competition,cultures
R1370 T2596 T2594 pobj cultures,in
R1371 T2597 T2575 punct .,investigated
R1372 T2599 T2600 amod Equal,numbers
R1373 T2600 T2601 nsubjpass numbers,inoculated
R1374 T2602 T2600 prep of,numbers
R1375 T2603 T2604 nmod Atrx,cells
R1376 T2604 T2602 pobj cells,of
R1377 T2605 T2603 punct +,Atrx
R1378 T2606 T2603 punct (,Atrx
R1379 T2607 T2603 acl bearing,Atrx
R1380 T2608 T2609 preconj either,Atrx WT
R1381 T2609 T2607 dobj Atrx WT,bearing
R1382 T2610 T2609 det an,Atrx WT
R1383 T2611 T2609 cc or,Atrx WT
R1384 T2612 T2613 det an,Atrx flox
R1385 T2613 T2614 compound Atrx flox,allele
R1386 T2614 T2609 conj allele,Atrx WT
R1387 T2615 T2603 punct ),Atrx
R1388 T2616 T2603 cc and,Atrx
R1389 T2617 T2603 conj Atrxnull,Atrx
R1390 T2618 T2617 punct (,Atrxnull
R1391 T2619 T2617 acl bearing,Atrxnull
R1392 T2620 T2621 det an,allele
R1393 T2621 T2619 dobj allele,bearing
R1394 T2622 T2621 compound Atrx Δ18Δneo,allele
R1395 T2623 T2604 punct ),cells
R1396 T2624 T2604 compound ES,cells
R1397 T2625 T2601 auxpass were,inoculated
R1398 T2626 T2601 prep into,inoculated
R1399 T2627 T2626 pobj cultures,into
R1400 T2628 T2601 cc and,inoculated
R1401 T2629 T2630 det the,cultures
R1402 T2630 T2632 nsubjpass cultures,passaged
R1403 T2631 T2630 amod mixed,cultures
R1404 T2632 T2601 conj passaged,inoculated
R1405 T2633 T2632 auxpass were,passaged
R1406 T2634 T2635 punct (,split
R1407 T2635 T2632 parataxis split,passaged
R1408 T2636 T2635 nummod 1,split
R1409 T2637 T2638 punct :,3
R1410 T2638 T2636 prep 3,1
R1411 T2639 T2635 punct ),split
R1412 T2640 T2641 det every,d
R1413 T2641 T2632 npadvmod d,passaged
R1414 T2642 T2641 nummod 2,d
R1415 T2643 T2632 prep for,passaged
R1416 T2644 T2645 quantmod 8,10
R1417 T2645 T2647 nummod 10,d
R1418 T2646 T2645 punct –,10
R1419 T2647 T2643 pobj d,for
R1420 T2648 T2632 punct .,passaged
R1421 T2650 T2651 det The,abundance
R1422 T2651 T2653 nsubjpass abundance,analysed
R1423 T2652 T2651 amod relative,abundance
R1424 T2654 T2651 prep of,abundance
R1425 T2655 T2656 det the,alleles
R1426 T2656 T2654 pobj alleles,of
R1427 T2657 T2656 amod different,alleles
R1428 T2658 T2651 prep in,abundance
R1429 T2659 T2660 det the,culture
R1430 T2660 T2658 pobj culture,in
R1431 T2661 T2651 prep at,abundance
R1432 T2662 T2663 det each,point
R1433 T2663 T2661 pobj point,at
R1434 T2664 T2663 compound time,point
R1435 T2665 T2653 auxpass was,analysed
R1436 T2666 T2653 prep by,analysed
R1437 T2667 T2668 compound Southern,blotting
R1438 T2668 T2666 pobj blotting,by
R1439 T2669 T2670 punct (,3A
R1440 T2670 T2653 parataxis 3A,analysed
R1441 T2671 T2670 compound Figure,3A
R1442 T2672 T2670 punct ),3A
R1443 T2673 T2653 punct .,analysed
R1444 T2675 T2676 det The,clone
R1445 T2676 T2677 nsubjpass clone,outgrown
R1446 T2678 T2676 acl containing,clone
R1447 T2679 T2680 det the,allele
R1448 T2680 T2678 dobj allele,containing
R1449 T2681 T2680 compound Atrx Δ18Δneo,allele
R1450 T2682 T2677 auxpass was,outgrown
R1451 T2683 T2677 advmod rapidly,outgrown
R1452 T2684 T2677 agent by,outgrown
R1453 T2685 T2686 preconj both,cells
R1454 T2686 T2684 pobj cells,by
R1455 T2687 T2686 compound Atrx WT,cells
R1456 T2688 T2686 compound ES,cells
R1457 T2689 T2686 cc and,cells
R1458 T2690 T2686 conj cells,cells
R1459 T2691 T2690 acl bearing,cells
R1460 T2692 T2693 det the,allele
R1461 T2693 T2691 dobj allele,bearing
R1462 T2694 T2693 compound Atrx flox,allele
R1463 T2695 T2677 punct .,outgrown
R1464 T2697 T2698 prep In,continued
R1465 T2699 T2700 det a,experiment
R1466 T2700 T2697 pobj experiment,In
R1467 T2701 T2700 compound control,experiment
R1468 T2702 T2700 compound competition,experiment
R1469 T2703 T2700 prep between,experiment
R1470 T2704 T2705 amod different,clones
R1471 T2705 T2703 pobj clones,between
R1472 T2706 T2705 acl bearing,clones
R1473 T2707 T2708 amod functional,alleles
R1474 T2708 T2706 dobj alleles,bearing
R1475 T2709 T2708 compound Atrx,alleles
R1476 T2710 T2708 punct (,alleles
R1477 T2711 T2708 appos Atrx WT,alleles
R1478 T2712 T2711 cc and,Atrx WT
R1479 T2713 T2711 conj Atrx flox,Atrx WT
R1480 T2714 T2698 punct ),continued
R1481 T2715 T2698 punct ", ",continued
R1482 T2716 T2717 det both,clones
R1483 T2717 T2698 nsubj clones,continued
R1484 T2718 T2719 aux to,be
R1485 T2719 T2698 xcomp be,continued
R1486 T2720 T2721 advmod equally,represented
R1487 T2721 T2719 acomp represented,be
R1488 T2722 T2719 prep after,be
R1489 T2723 T2724 nummod 8,d
R1490 T2724 T2722 pobj d,after
R1491 T2725 T2724 prep of,d
R1492 T2726 T2725 pobj cocultivation,of
R1493 T2727 T2698 punct .,continued
R1494 T2729 T2730 advmod Thus,suggested
R1495 T2731 T2730 punct ", ",suggested
R1496 T2732 T2733 mark although,recovered
R1497 T2733 T2730 advcl recovered,suggested
R1498 T2734 T2735 amod Atrxnull,cells
R1499 T2735 T2733 nsubj cells,recovered
R1500 T2736 T2735 compound ES,cells
R1501 T2737 T2733 aux could,recovered
R1502 T2738 T2733 aux be,recovered
R1503 T2739 T2733 cc and,recovered
R1504 T2740 T2733 conj maintained,recovered
R1505 T2741 T2740 prep in,maintained
R1506 T2742 T2741 pobj culture,in
R1507 T2743 T2733 prep by,recovered
R1508 T2744 T2745 det a,approach
R1509 T2745 T2743 pobj approach,by
R1510 T2746 T2747 amod conditional,targeting
R1511 T2747 T2745 compound targeting,approach
R1512 T2748 T2730 punct ", ",suggested
R1513 T2749 T2750 det these,experiments
R1514 T2750 T2730 nsubj experiments,suggested
R1515 T2751 T2750 compound cocultivation,experiments
R1516 T2752 T2753 mark that,impact
R1517 T2753 T2730 ccomp impact,suggested
R1518 T2754 T2755 det the,absence
R1519 T2755 T2753 nsubj absence,impact
R1520 T2756 T2755 prep of,absence
R1521 T2757 T2756 pobj Atrx,of
R1522 T2758 T2753 aux does,impact
R1523 T2759 T2753 advmod negatively,impact
R1524 T2760 T2753 prep upon,impact
R1525 T2761 T2762 amod normal,growth
R1526 T2762 T2760 pobj growth,upon
R1527 T2763 T2764 compound ES,cell
R1528 T2764 T2762 compound cell,growth
R1529 T2765 T2730 punct .,suggested
R1530 T2767 T2768 aux To,investigate
R1531 T2768 T2769 advcl investigate,analysed
R1532 T2770 T2771 det a,defect
R1533 T2771 T2768 dobj defect,investigate
R1534 T2772 T2771 amod possible,defect
R1535 T2773 T2774 compound cell,cycle
R1536 T2774 T2771 compound cycle,defect
R1537 T2775 T2774 punct -,cycle
R1538 T2776 T2771 prep in,defect
R1539 T2777 T2778 det the,absence
R1540 T2778 T2776 pobj absence,in
R1541 T2779 T2778 prep of,absence
R1542 T2780 T2779 pobj Atrx,of
R1543 T2781 T2769 punct ", ",analysed
R1544 T2782 T2769 nsubj we,analysed
R1545 T2783 T2784 det the,distribution
R1546 T2784 T2769 dobj distribution,analysed
R1547 T2785 T2786 compound cell,cycle
R1548 T2786 T2784 compound cycle,distribution
R1549 T2787 T2784 prep of,distribution
R1550 T2788 T2789 npadvmod bromodeoxyuridine,pulsed
R1551 T2789 T2794 amod pulsed,cells
R1552 T2790 T2788 punct (,bromodeoxyuridine
R1553 T2791 T2788 appos BrdU,bromodeoxyuridine
R1554 T2792 T2789 punct ),pulsed
R1555 T2793 T2789 punct -,pulsed
R1556 T2794 T2787 pobj cells,of
R1557 T2795 T2794 compound ES,cells
R1558 T2796 T2769 prep by,analysed
R1559 T2797 T2798 compound flow,cytometry
R1560 T2798 T2796 pobj cytometry,by
R1561 T2799 T2800 punct (,S1A
R1562 T2800 T2769 parataxis S1A,analysed
R1563 T2801 T2800 compound Figure,S1A
R1564 T2802 T2800 punct ),S1A
R1565 T2803 T2769 punct .,analysed
R1566 T2805 T2806 advmod Surprisingly,exhibited
R1567 T2807 T2806 punct ", ",exhibited
R1568 T2808 T2809 det both,clones
R1569 T2809 T2806 nsubj clones,exhibited
R1570 T2810 T2811 amod Atrxnull,cell
R1571 T2811 T2809 compound cell,clones
R1572 T2812 T2811 compound ES,cell
R1573 T2813 T2814 det a,profile
R1574 T2814 T2806 dobj profile,exhibited
R1575 T2815 T2816 compound cell,cycle
R1576 T2816 T2814 compound cycle,profile
R1577 T2817 T2818 dep that,was
R1578 T2818 T2814 relcl was,profile
R1579 T2819 T2818 acomp indistinguishable,was
R1580 T2820 T2819 prep from,indistinguishable
R1581 T2821 T2822 compound ES,cells
R1582 T2822 T2820 pobj cells,from
R1583 T2823 T2822 acl bearing,cells
R1584 T2824 T2825 det a,allele
R1585 T2825 T2823 dobj allele,bearing
R1586 T2826 T2825 amod functional,allele
R1587 T2827 T2825 compound Atrx,allele
R1588 T2828 T2825 punct (,allele
R1589 T2829 T2825 appos Atrx WT,allele
R1590 T2830 T2829 cc or,Atrx WT
R1591 T2831 T2829 conj Atrx flox,Atrx WT
R1592 T2832 T2806 punct ),exhibited
R1593 T2833 T2806 punct .,exhibited
R1594 T2835 T2836 nsubj We,quantitated
R1595 T2837 T2836 advmod also,quantitated
R1596 T2838 T2836 advmod specifically,quantitated
R1597 T2839 T2840 det the,index
R1598 T2840 T2836 dobj index,quantitated
R1599 T2841 T2840 amod mitotic,index
R1600 T2842 T2840 prep within,index
R1601 T2843 T2844 det each,population
R1602 T2844 T2842 pobj population,within
R1603 T2845 T2836 prep by,quantitated
R1604 T2846 T2847 compound flow,cytometry
R1605 T2847 T2845 pobj cytometry,by
R1606 T2848 T2836 prep after,quantitated
R1607 T2849 T2848 pcomp staining,after
R1608 T2850 T2851 compound ES,cells
R1609 T2851 T2849 dobj cells,staining
R1610 T2852 T2849 prep for,staining
R1611 T2853 T2854 amod phosphorylated,H3
R1612 T2854 T2852 pobj H3,for
R1613 T2855 T2856 punct (,Ser10
R1614 T2856 T2853 parataxis Ser10,phosphorylated
R1615 T2857 T2856 punct ),Ser10
R1616 T2858 T2854 compound histone,H3
R1617 T2859 T2854 punct ", ",H3
R1618 T2860 T2861 det a,marker
R1619 T2861 T2854 appos marker,H3
R1620 T2862 T2861 amod specific,marker
R1621 T2863 T2861 prep of,marker
R1622 T2864 T2863 pobj mitosis,of
R1623 T2865 T2866 punct (,S1B
R1624 T2866 T2836 parataxis S1B,quantitated
R1625 T2867 T2866 compound Figure,S1B
R1626 T2868 T2866 punct ),S1B
R1627 T2869 T2870 punct [,13
R1628 T2870 T2836 parataxis 13,quantitated
R1629 T2871 T2870 punct ],13
R1630 T2872 T2836 punct .,quantitated
R1631 T2874 T2875 advcl Consistent,was
R1632 T2876 T2874 prep with,Consistent
R1633 T2877 T2878 det the,profile
R1634 T2878 T2876 pobj profile,with
R1635 T2879 T2878 amod normal,profile
R1636 T2880 T2881 compound cell,cycle
R1637 T2881 T2878 compound cycle,profile
R1638 T2882 T2881 punct -,cycle
R1639 T2883 T2878 acl observed,profile
R1640 T2884 T2883 advmod above,observed
R1641 T2885 T2875 punct ", ",was
R1642 T2886 T2875 expl there,was
R1643 T2887 T2888 det no,depletion
R1644 T2888 T2875 attr depletion,was
R1645 T2889 T2888 prep in,depletion
R1646 T2890 T2891 det the,size
R1647 T2891 T2889 pobj size,in
R1648 T2892 T2891 prep of,size
R1649 T2893 T2894 det the,population
R1650 T2894 T2892 pobj population,of
R1651 T2895 T2894 amod mitotic,population
R1652 T2896 T2875 prep in,was
R1653 T2897 T2898 det the,clones
R1654 T2898 T2896 pobj clones,in
R1655 T2899 T2898 amod Atrxnull,clones
R1656 T2900 T2898 compound ES,clones
R1657 T2901 T2875 punct ", ",was
R1658 T2902 T2875 prep despite,was
R1659 T2903 T2904 poss their,growth
R1660 T2904 T2902 pobj growth,despite
R1661 T2905 T2904 amod slow,growth
R1662 T2906 T2875 punct .,was
R1663 T2908 T2909 advmod Finally,investigated
R1664 T2910 T2909 punct ", ",investigated
R1665 T2911 T2909 nsubj we,investigated
R1666 T2912 T2913 mark whether,was
R1667 T2913 T2909 ccomp was,investigated
R1668 T2914 T2915 det the,defect
R1669 T2915 T2913 nsubj defect,was
R1670 T2916 T2915 compound growth,defect
R1671 T2917 T2915 prep in,defect
R1672 T2918 T2919 det the,cells
R1673 T2919 T2917 pobj cells,in
R1674 T2920 T2921 amod Atrxnull,ES
R1675 T2921 T2919 compound ES,cells
R1676 T2922 T2913 prep due,was
R1677 T2923 T2922 pcomp to,due
R1678 T2924 T2925 det an,regulation
R1679 T2925 T2922 pobj regulation,due
R1680 T2926 T2925 amod up,regulation
R1681 T2927 T2925 punct -,regulation
R1682 T2928 T2925 prep of,regulation
R1683 T2929 T2928 pobj apoptosis,of
R1684 T2930 T2909 prep by,investigated
R1685 T2931 T2930 pcomp staining,by
R1686 T2932 T2931 dobj cells,staining
R1687 T2933 T2931 prep with,staining
R1688 T2934 T2933 pobj Annexin,with
R1689 T2935 T2934 nummod V,Annexin
R1690 T2936 T2937 punct (,S2
R1691 T2937 T2909 parataxis S2,investigated
R1692 T2938 T2937 compound Figure,S2
R1693 T2939 T2937 punct ),S2
R1694 T2940 T2909 cc and,investigated
R1695 T2941 T2909 conj found,investigated
R1696 T2942 T2943 mark that,affected
R1697 T2943 T2941 ccomp affected,found
R1698 T2944 T2945 det the,proportion
R1699 T2945 T2943 nsubjpass proportion,affected
R1700 T2946 T2945 prep of,proportion
R1701 T2947 T2948 amod apoptotic,cells
R1702 T2948 T2946 pobj cells,of
R1703 T2949 T2943 auxpass was,affected
R1704 T2950 T2943 neg not,affected
R1705 T2951 T2943 advmod significantly,affected
R1706 T2952 T2943 agent by,affected
R1707 T2953 T2954 det the,absence
R1708 T2954 T2952 pobj absence,by
R1709 T2955 T2954 prep of,absence
R1710 T2956 T2957 amod full,length
R1711 T2957 T2959 compound length,Atrx
R1712 T2958 T2957 punct -,length
R1713 T2959 T2955 pobj Atrx,of
R1714 T2960 T2909 punct .,investigated
R1715 T2962 T2963 advmod Thus,is
R1716 T2964 T2963 punct ", ",is
R1717 T2965 T2966 det the,defect
R1718 T2966 T2963 nsubj defect,is
R1719 T2967 T2966 compound growth,defect
R1720 T2968 T2966 acl observed,defect
R1721 T2969 T2968 prep in,observed
R1722 T2970 T2971 compound ES,cells
R1723 T2971 T2969 pobj cells,in
R1724 T2972 T2971 acl lacking,cells
R1725 T2973 T2972 dobj Atrx,lacking
R1726 T2974 T2963 neg not,is
R1727 T2975 T2963 prep due,is
R1728 T2976 T2975 pcomp to,due
R1729 T2977 T2978 det a,block
R1730 T2978 T2975 pobj block,due
R1731 T2979 T2978 amod specific,block
R1732 T2980 T2981 compound cell,cycle
R1733 T2981 T2978 compound cycle,block
R1734 T2982 T2978 cc or,block
R1735 T2983 T2984 amod significant,induction
R1736 T2984 T2978 conj induction,block
R1737 T2985 T2984 prep of,induction
R1738 T2986 T2987 compound cell,death
R1739 T2987 T2985 pobj death,of
R1740 T2988 T2963 punct .,is
R1741 T2990 T2991 mark While,is
R1742 T2991 T2998 advcl is,seems
R1743 T2992 T2993 det the,cause
R1744 T2993 T2991 nsubj cause,is
R1745 T2994 T2993 prep of,cause
R1746 T2995 T2996 det the,delay
R1747 T2996 T2994 pobj delay,of
R1748 T2997 T2996 amod proliferative,delay
R1749 T2999 T2991 neg not,is
R1750 T3000 T2991 advmod yet,is
R1751 T3001 T2991 acomp clear,is
R1752 T3002 T2991 punct ", ",is
R1753 T3003 T3004 mark since,appear
R1754 T3004 T2991 advcl appear,is
R1755 T3005 T3006 amod Atrxnull,cells
R1756 T3006 T3004 nsubj cells,appear
R1757 T3007 T3006 compound ES,cells
R1758 T3008 T3009 aux to,undergo
R1759 T3009 T3004 xcomp undergo,appear
R1760 T3010 T3011 amod higher,rates
R1761 T3011 T3009 dobj rates,undergo
R1762 T3012 T3011 prep of,rates
R1763 T3013 T3014 amod spontaneous,differentiation
R1764 T3014 T3012 pobj differentiation,of
R1765 T3015 T3016 punct (,data
R1766 T3016 T2991 meta data,is
R1767 T3017 T3016 amod unpublished,data
R1768 T3018 T3016 punct ),data
R1769 T3019 T2998 punct ", ",seems
R1770 T3020 T2998 nsubj it,seems
R1771 T3021 T2998 oprd likely,seems
R1772 T3022 T3023 mark that,reflects
R1773 T3023 T2998 ccomp reflects,seems
R1774 T3024 T3025 det the,defect
R1775 T3025 T3023 nsubj defect,reflects
R1776 T3026 T3025 amod observed,defect
R1777 T3027 T3025 compound growth,defect
R1778 T3028 T3029 det the,transition
R1779 T3029 T3023 dobj transition,reflects
R1780 T3030 T3029 amod spontaneous,transition
R1781 T3031 T3029 prep from,transition
R1782 T3032 T3033 advmod fast,cycling
R1783 T3033 T3035 amod cycling,cells
R1784 T3034 T3033 punct -,cycling
R1785 T3035 T3031 pobj cells,from
R1786 T3036 T3035 punct ", ",cells
R1787 T3037 T3035 amod undifferentiated,cells
R1788 T3038 T3035 compound ES,cells
R1789 T3039 T3029 prep into,transition
R1790 T3040 T3041 advmod more,slowly
R1791 T3041 T3042 advmod slowly,cycling
R1792 T3042 T3043 amod cycling,types
R1793 T3043 T3039 pobj types,into
R1794 T3044 T3043 punct ", ",types
R1795 T3045 T3043 amod differentiated,types
R1796 T3046 T3043 compound cell,types
R1797 T3047 T3023 prep in,reflects
R1798 T3048 T3049 det these,cultures
R1799 T3049 T3047 pobj cultures,in
R1800 T3050 T2998 punct .,seems
R1801 T3052 T3053 nsubjpass It,shown
R1802 T3054 T3053 aux has,shown
R1803 T3055 T3053 auxpass been,shown
R1804 T3056 T3057 mark that,give
R1805 T3057 T3053 ccomp give,shown
R1806 T3058 T3059 npadvmod disease,causing
R1807 T3059 T3061 amod causing,mutations
R1808 T3060 T3059 punct -,causing
R1809 T3061 T3057 nsubj mutations,give
R1810 T3062 T3061 prep in,mutations
R1811 T3063 T3064 det the,gene
R1812 T3064 T3062 pobj gene,in
R1813 T3065 T3064 amod human,gene
R1814 T3066 T3064 compound ATRX,gene
R1815 T3067 T3057 dobj rise,give
R1816 T3068 T3057 prep to,give
R1817 T3069 T3068 pobj changes,to
R1818 T3070 T3069 prep in,changes
R1819 T3071 T3072 det the,pattern
R1820 T3072 T3070 pobj pattern,in
R1821 T3073 T3072 amod normal,pattern
R1822 T3074 T3072 prep of,pattern
R1823 T3075 T3076 compound DNA,methylation
R1824 T3076 T3074 pobj methylation,of
R1825 T3077 T3069 prep at,changes
R1826 T3078 T3079 amod several,sequences
R1827 T3079 T3077 pobj sequences,at
R1828 T3080 T3079 amod repetitive,sequences
R1829 T3081 T3079 prep within,sequences
R1830 T3082 T3083 det the,genome
R1831 T3083 T3081 pobj genome,within
R1832 T3084 T3083 amod human,genome
R1833 T3085 T3086 punct [,11
R1834 T3086 T3057 parataxis 11,give
R1835 T3087 T3086 punct ],11
R1836 T3088 T3053 punct .,shown
R1837 T3090 T3091 advmod Notably,found
R1838 T3092 T3091 punct ", ",found
R1839 T3093 T3094 det the,region
R1840 T3094 T3091 nsubjpass region,found
R1841 T3095 T3094 amod transcribed,region
R1842 T3096 T3094 prep of,region
R1843 T3097 T3098 det the,repeat
R1844 T3098 T3096 pobj repeat,of
R1845 T3099 T3100 amod ribosomal,DNA
R1846 T3100 T3098 nmod DNA,repeat
R1847 T3101 T3100 punct (,DNA
R1848 T3102 T3100 appos rDNA,DNA
R1849 T3103 T3098 punct ),repeat
R1850 T3104 T3091 auxpass was,found
R1851 T3105 T3106 aux to,hypomethylated
R1852 T3106 T3091 xcomp hypomethylated,found
R1853 T3107 T3106 auxpass be,hypomethylated
R1854 T3108 T3106 advmod significantly,hypomethylated
R1855 T3109 T3106 prep in,hypomethylated
R1856 T3110 T3111 compound ATR,X
R1857 T3111 T3113 compound X,patients
R1858 T3112 T3111 punct -,X
R1859 T3113 T3109 pobj patients,in
R1860 T3114 T3106 advcl relative,hypomethylated
R1861 T3115 T3114 prep to,relative
R1862 T3116 T3117 amod normal,individuals
R1863 T3117 T3115 pobj individuals,to
R1864 T3118 T3091 punct .,found
R1865 T3120 T3121 advcl Using,observed
R1866 T3122 T3123 npadvmod methylation,sensitive
R1867 T3123 T3125 amod sensitive,enzymes
R1868 T3124 T3123 punct -,sensitive
R1869 T3125 T3120 dobj enzymes,Using
R1870 T3126 T3125 compound restriction,enzymes
R1871 T3127 T3121 punct ", ",observed
R1872 T3128 T3121 nsubj we,observed
R1873 T3129 T3121 advmod also,observed
R1874 T3130 T3131 amod significant,hypomethylation
R1875 T3131 T3121 dobj hypomethylation,observed
R1876 T3132 T3121 prep at,observed
R1877 T3133 T3134 amod several,sites
R1878 T3134 T3132 pobj sites,at
R1879 T3135 T3134 acl tested,sites
R1880 T3136 T3135 prep within,tested
R1881 T3137 T3138 det the,repeats
R1882 T3138 T3136 pobj repeats,within
R1883 T3139 T3140 compound mouse,rDNA
R1884 T3140 T3138 compound rDNA,repeats
R1885 T3141 T3138 prep in,repeats
R1886 T3142 T3143 amod Atrxnull,cells
R1887 T3143 T3141 pobj cells,in
R1888 T3144 T3143 compound ES,cells
R1889 T3145 T3143 cc and,cells
R1890 T3146 T3147 nummod 12,d
R1891 T3147 T3149 nmod d,bodies
R1892 T3148 T3147 punct -,d
R1893 T3149 T3143 conj bodies,cells
R1894 T3150 T3149 amod embryoid,bodies
R1895 T3151 T3121 advcl relative,observed
R1896 T3152 T3151 prep to,relative
R1897 T3153 T3154 compound ES,cells
R1898 T3154 T3152 pobj cells,to
R1899 T3155 T3154 cc and,cells
R1900 T3156 T3157 amod embryoid,bodies
R1901 T3157 T3154 conj bodies,cells
R1902 T3158 T3154 acl bearing,cells
R1903 T3159 T3160 det a,allele
R1904 T3160 T3158 dobj allele,bearing
R1905 T3161 T3160 amod functional,allele
R1906 T3162 T3160 compound Atrx,allele
R1907 T3163 T3160 punct (,allele
R1908 T3164 T3160 appos Atrx WT,allele
R1909 T3165 T3164 cc or,Atrx WT
R1910 T3166 T3164 conj Atrx flox,Atrx WT
R1911 T3167 T3121 punct ),observed
R1912 T3168 T3169 punct (,3B
R1913 T3169 T3121 parataxis 3B,observed
R1914 T3170 T3169 compound Figure,3B
R1915 T3171 T3169 cc and,3B
R1916 T3172 T3169 conj 3C,3B
R1917 T3173 T3169 punct ),3B
R1918 T3174 T3121 punct .,observed
R1919 T3176 T3177 det The,observation
R1920 T3177 T3178 nsubj observation,is
R1921 T3179 T3180 mark that,hypomethylated
R1922 T3180 T3177 acl hypomethylated,observation
R1923 T3181 T3180 nsubjpass rDNA,hypomethylated
R1924 T3182 T3180 auxpass is,hypomethylated
R1925 T3183 T3180 prep in,hypomethylated
R1926 T3184 T3185 det the,absence
R1927 T3185 T3183 pobj absence,in
R1928 T3186 T3185 prep of,absence
R1929 T3187 T3186 pobj Atrx,of
R1930 T3188 T3180 punct ", ",hypomethylated
R1931 T3189 T3190 advmod even,in
R1932 T3190 T3180 prep in,hypomethylated
R1933 T3191 T3192 compound ES,cells
R1934 T3192 T3190 pobj cells,in
R1935 T3193 T3178 punct ", ",is
R1936 T3194 T3178 acomp consistent,is
R1937 T3195 T3194 prep with,consistent
R1938 T3196 T3197 det the,finding
R1939 T3197 T3195 pobj finding,with
R1940 T3198 T3199 mark that,is
R1941 T3199 T3197 acl is,finding
R1942 T3200 T3199 nsubj hypomethylation,is
R1943 T3201 T3200 prep of,hypomethylation
R1944 T3202 T3203 det the,repeats
R1945 T3203 T3201 pobj repeats,of
R1946 T3204 T3205 amod human,rDNA
R1947 T3205 T3203 compound rDNA,repeats
R1948 T3206 T3199 acomp detectable,is
R1949 T3207 T3199 prep from,is
R1950 T3208 T3209 det an,stage
R1951 T3209 T3207 pobj stage,from
R1952 T3210 T3209 amod early,stage
R1953 T3211 T3209 amod developmental,stage
R1954 T3212 T3199 prep in,is
R1955 T3213 T3214 compound ATR,X
R1956 T3214 T3216 compound X,patients
R1957 T3215 T3214 punct -,X
R1958 T3216 T3212 pobj patients,in
R1959 T3217 T3178 punct .,is
R1960 T3219 T3220 amod Other,elements
R1961 T3220 T3224 nsubj elements,include
R1962 T3221 T3220 nmod mouse,elements
R1963 T3222 T3223 amod repetitive,sequence
R1964 T3223 T3220 compound sequence,elements
R1965 T3225 T3220 acl surveyed,elements
R1966 T3226 T3225 prep in,surveyed
R1967 T3227 T3228 compound ES,DNA
R1968 T3228 T3226 pobj DNA,in
R1969 T3229 T3228 compound cell,DNA
R1970 T3230 T3231 det the,repeats
R1971 T3231 T3224 dobj repeats,include
R1972 T3232 T3233 amod heterochromatic,satellite
R1973 T3233 T3231 nmod satellite,repeats
R1974 T3234 T3233 amod major,satellite
R1975 T3235 T3236 punct (,assayed
R1976 T3236 T3233 parataxis assayed,satellite
R1977 T3237 T3236 prep with,assayed
R1978 T3238 T3237 pobj MaeII,with
R1979 T3239 T3236 punct ),assayed
R1980 T3240 T3233 cc and,satellite
R1981 T3241 T3242 amod minor,satellite
R1982 T3242 T3233 conj satellite,satellite
R1983 T3243 T3244 punct (,assayed
R1984 T3244 T3242 parataxis assayed,satellite
R1985 T3245 T3244 prep with,assayed
R1986 T3246 T3245 pobj HpaII,with
R1987 T3247 T3244 punct ),assayed
R1988 T3248 T3231 punct ", ",repeats
R1989 T3249 T3250 advmod as,as
R1990 T3250 T3231 cc as,repeats
R1991 T3251 T3250 advmod well,as
R1992 T3252 T3253 amod interspersed,repeats
R1993 T3253 T3231 conj repeats,repeats
R1994 T3254 T3253 amod retroviral,repeats
R1995 T3255 T3253 prep of,repeats
R1996 T3256 T3257 det the,type
R1997 T3257 T3255 pobj type,of
R1998 T3258 T3259 amod intracisternal,particle
R1999 T3259 T3257 nmod particle,type
R2000 T3260 T3259 nmod A,particle
R2001 T3261 T3259 punct (,particle
R2002 T3262 T3259 appos IAP,particle
R2003 T3263 T3257 punct ),type
R2004 T3264 T3257 cc and,type
R2005 T3265 T3266 det the,families
R2006 T3266 T3257 conj families,type
R2007 T3267 T3266 nmod Line,families
R2008 T3268 T3267 nummod 1,Line
R2009 T3269 T3267 cc and,Line
R2010 T3270 T3271 compound Sine,B1
R2011 T3271 T3267 conj B1,Line
R2012 T3272 T3273 punct (,all
R2013 T3273 T3266 parataxis all,families
R2014 T3274 T3273 acl assayed,all
R2015 T3275 T3274 prep with,assayed
R2016 T3276 T3275 pobj HpaII,with
R2017 T3277 T3273 punct ),all
R2018 T3278 T3224 punct .,include
R2019 T3280 T3281 det These,repeats
R2020 T3281 T3282 nsubjpass repeats,found
R2021 T3283 T3282 auxpass were,found
R2022 T3284 T3285 aux to,be
R2023 T3285 T3282 xcomp be,found
R2024 T3286 T3287 advmod moderately,methylated
R2025 T3287 T3285 acomp methylated,be
R2026 T3288 T3289 punct (,Line
R2027 T3289 T3286 parataxis Line,moderately
R2028 T3290 T3289 nummod 1,Line
R2029 T3291 T3289 cc and,Line
R2030 T3292 T3293 compound Sine,B1
R2031 T3293 T3289 conj B1,Line
R2032 T3294 T3289 punct ),Line
R2033 T3295 T3286 cc or,moderately
R2034 T3296 T3286 conj highly,moderately
R2035 T3297 T3298 punct (,IAP
R2036 T3298 T3296 parataxis IAP,highly
R2037 T3299 T3298 punct ", ",IAP
R2038 T3300 T3301 amod major,satellite
R2039 T3301 T3298 appos satellite,IAP
R2040 T3302 T3298 punct ", ",IAP
R2041 T3303 T3304 amod minor,satellite
R2042 T3304 T3298 appos satellite,IAP
R2043 T3305 T3298 punct ),IAP
R2044 T3306 T3285 prep in,be
R2045 T3307 T3308 amod wild,type
R2046 T3308 T3310 compound type,cells
R2047 T3309 T3308 punct -,type
R2048 T3310 T3306 pobj cells,in
R2049 T3311 T3310 compound ES,cells
R2050 T3312 T3282 punct ", ",found
R2051 T3313 T3282 cc and,found
R2052 T3314 T3315 det this,methylation
R2053 T3315 T3316 nsubjpass methylation,perturbed
R2054 T3316 T3282 conj perturbed,found
R2055 T3317 T3316 auxpass was,perturbed
R2056 T3318 T3316 neg not,perturbed
R2057 T3319 T3316 advmod detectably,perturbed
R2058 T3320 T3316 agent by,perturbed
R2059 T3321 T3322 det the,absence
R2060 T3322 T3320 pobj absence,by
R2061 T3323 T3322 prep of,absence
R2062 T3324 T3323 pobj Atrx,of
R2063 T3325 T3326 punct (,S3
R2064 T3326 T3316 parataxis S3,perturbed
R2065 T3327 T3326 compound Figure,S3
R2066 T3328 T3326 cc and,S3
R2067 T3329 T3330 amod unpublished,data
R2068 T3330 T3326 conj data,S3
R2069 T3331 T3326 punct ),S3
R2070 T3332 T3316 punct .,perturbed
R2071 T3334 T3335 advcl Taken,indicate
R2072 T3336 T3334 advmod together,Taken
R2073 T3337 T3335 punct ", ",indicate
R2074 T3338 T3339 det these,data
R2075 T3339 T3335 nsubj data,indicate
R2076 T3340 T3341 mark that,is
R2077 T3341 T3335 ccomp is,indicate
R2078 T3342 T3343 det the,interplay
R2079 T3343 T3341 nsubj interplay,is
R2080 T3344 T3343 amod subtle,interplay
R2081 T3345 T3343 prep between,interplay
R2082 T3346 T3347 det the,protein
R2083 T3347 T3345 pobj protein,between
R2084 T3348 T3347 compound ATRX,protein
R2085 T3349 T3347 cc and,protein
R2086 T3350 T3351 compound DNA,methylation
R2087 T3351 T3347 conj methylation,protein
R2088 T3352 T3343 acl observed,interplay
R2089 T3353 T3352 prep in,observed
R2090 T3354 T3355 amod human,patients
R2091 T3355 T3353 pobj patients,in
R2092 T3356 T3341 advmod also,is
R2093 T3357 T3341 acomp present,is
R2094 T3358 T3341 prep in,is
R2095 T3359 T3360 compound mouse,cells
R2096 T3360 T3358 pobj cells,in
R2097 T3361 T3335 punct .,indicate
R2105 T3956 T3957 amod Early,Lethality
R2106 T3958 T3957 amod Embryonic,Lethality
R2107 T3959 T3957 prep in,Lethality
R2108 T3960 T3961 amod Atrxnull,Mice
R2109 T3961 T3959 pobj Mice,in
R2110 T3962 T3961 amod Male,Mice
R2111 T3964 T3965 aux To,investigate
R2112 T3965 T3966 advcl investigate,established
R2113 T3967 T3968 det the,role
R2114 T3968 T3965 dobj role,investigate
R2115 T3969 T3968 prep of,role
R2116 T3970 T3971 det the,protein
R2117 T3971 T3969 pobj protein,of
R2118 T3972 T3971 compound Atrx,protein
R2119 T3973 T3968 prep during,role
R2120 T3974 T3975 compound mouse,development
R2121 T3975 T3973 pobj development,during
R2122 T3976 T3966 punct ", ",established
R2123 T3977 T3966 nsubj we,established
R2124 T3978 T3966 advmod initially,established
R2125 T3979 T3966 dobj lines,established
R2126 T3980 T3979 prep of,lines
R2127 T3981 T3980 pobj mice,of
R2128 T3982 T3979 acl bearing,lines
R2129 T3983 T3984 det the,allele
R2130 T3984 T3982 dobj allele,bearing
R2131 T3985 T3984 compound Atrx flox,allele
R2132 T3986 T3966 punct .,established
R2133 T3988 T3989 nummod Two,clones
R2134 T3989 T3995 nsubjpass clones,injected
R2135 T3990 T3991 advmod independently,targeted
R2136 T3991 T3989 amod targeted,clones
R2137 T3992 T3989 compound Atrx flox,clones
R2138 T3993 T3994 compound ES,cell
R2139 T3994 T3989 compound cell,clones
R2140 T3996 T3989 prep with,clones
R2141 T3997 T3998 amod normal,karyotype
R2142 T3998 T3996 pobj karyotype,with
R2143 T3999 T3998 amod male,karyotype
R2144 T4000 T3995 auxpass were,injected
R2145 T4001 T3995 prep into,injected
R2146 T4002 T4003 nmod C57BL,blastocysts
R2147 T4003 T4001 pobj blastocysts,into
R2148 T4004 T4002 punct /,C57BL
R2149 T4005 T4002 nummod 6,C57BL
R2150 T4006 T4007 aux to,produce
R2151 T4007 T3995 advcl produce,injected
R2152 T4008 T4009 amod chimaeric,mice
R2153 T4009 T4007 dobj mice,produce
R2154 T4010 T4009 punct ", ",mice
R2155 T4011 T4012 dep which,used
R2156 T4012 T4009 relcl used,mice
R2157 T4013 T4012 auxpass were,used
R2158 T4014 T4012 advmod then,used
R2159 T4015 T4016 aux to,obtain
R2160 T4016 T4012 advcl obtain,used
R2161 T4017 T4018 compound germline,transmission
R2162 T4018 T4016 dobj transmission,obtain
R2163 T4019 T3995 punct .,injected
R2164 T4021 T4022 nsubjpass Intercrosses,carried
R2165 T4023 T4021 prep between,Intercrosses
R2166 T4024 T4023 pobj males,between
R2167 T4025 T4024 amod hemizygous,males
R2168 T4026 T4027 punct (,Y
R2169 T4027 T4025 parataxis Y,hemizygous
R2170 T4028 T4027 compound Atrx flox,Y
R2171 T4029 T4027 punct /,Y
R2172 T4030 T4027 punct ),Y
R2173 T4031 T4024 cc and,males
R2174 T4032 T4024 conj females,males
R2175 T4033 T4032 amod heterozygous,females
R2176 T4034 T4035 punct (,flox
R2177 T4035 T4033 parataxis flox,heterozygous
R2178 T4036 T4035 compound Atrx WT,flox
R2179 T4037 T4035 punct /,flox
R2180 T4038 T4035 punct ),flox
R2181 T4039 T4032 prep for,females
R2182 T4040 T4041 det the,allele
R2183 T4041 T4039 pobj allele,for
R2184 T4042 T4041 amod floxed,allele
R2185 T4043 T4022 auxpass were,carried
R2186 T4044 T4022 advmod also,carried
R2187 T4045 T4022 prt out,carried
R2188 T4046 T4047 aux to,generate
R2189 T4047 T4022 advcl generate,carried
R2190 T4048 T4049 amod homozygous,females
R2191 T4049 T4047 dobj females,generate
R2192 T4050 T4051 punct (,flox
R2193 T4051 T4049 parataxis flox,females
R2194 T4052 T4051 compound Atrx flox,flox
R2195 T4053 T4051 punct /,flox
R2196 T4054 T4051 punct ),flox
R2197 T4055 T4022 punct .,carried
R2198 T4057 T4058 nsubj Males,were
R2199 T4059 T4057 amod hemizygous,Males
R2200 T4060 T4057 cc and,Males
R2201 T4061 T4057 conj females,Males
R2202 T4062 T4061 amod heterozygous,females
R2203 T4063 T4062 cc or,heterozygous
R2204 T4064 T4062 conj homozygous,heterozygous
R2205 T4065 T4061 prep for,females
R2206 T4066 T4067 det the,allele
R2207 T4067 T4065 pobj allele,for
R2208 T4068 T4067 compound Atrx flox,allele
R2209 T4069 T4058 acomp viable,were
R2210 T4070 T4058 punct ", ",were
R2211 T4071 T4058 conj appeared,were
R2212 T4072 T4071 oprd healthy,appeared
R2213 T4073 T4071 punct ", ",appeared
R2214 T4074 T4071 cc and,appeared
R2215 T4075 T4071 conj bred,appeared
R2216 T4076 T4075 advmod normally,bred
R2217 T4077 T4058 punct ", ",were
R2218 T4078 T4058 advcl suggesting,were
R2219 T4079 T4080 mark that,was
R2220 T4080 T4078 ccomp was,suggesting
R2221 T4081 T4080 punct ", ",was
R2222 T4082 T4083 mark as,expected
R2223 T4083 T4080 advcl expected,was
R2224 T4084 T4080 punct ", ",was
R2225 T4085 T4086 det the,allele
R2226 T4086 T4080 nsubj allele,was
R2227 T4087 T4086 compound Atrx flox,allele
R2228 T4088 T4089 advmod functionally,normal
R2229 T4089 T4080 acomp normal,was
R2230 T4090 T4058 punct .,were
R2231 T4092 T4093 aux To,generate
R2232 T4093 T4094 advcl generate,crossed
R2233 T4095 T4096 amod Atrxnull,mice
R2234 T4096 T4093 dobj mice,generate
R2235 T4097 T4093 prep by,generate
R2236 T4098 T4099 npadvmod Cre,mediated
R2237 T4099 T4101 amod mediated,recombination
R2238 T4100 T4099 punct -,mediated
R2239 T4101 T4097 pobj recombination,by
R2240 T4102 T4094 punct ", ",crossed
R2241 T4103 T4104 det the,mice
R2242 T4104 T4094 nsubjpass mice,crossed
R2243 T4105 T4104 compound Atrx flox,mice
R2244 T4106 T4094 auxpass were,crossed
R2245 T4107 T4094 prep with,crossed
R2246 T4108 T4107 pobj mice,with
R2247 T4109 T4108 acl harboring,mice
R2248 T4110 T4111 det a,transgene
R2249 T4111 T4109 dobj transgene,harboring
R2250 T4112 T4113 prep in,expressed
R2251 T4113 T4111 relcl expressed,transgene
R2252 T4114 T4112 pobj which,in
R2253 T4115 T4116 det the,recombinase
R2254 T4116 T4113 nsubjpass recombinase,expressed
R2255 T4117 T4116 compound Cre,recombinase
R2256 T4118 T4113 auxpass is,expressed
R2257 T4119 T4113 prep under,expressed
R2258 T4120 T4121 det the,control
R2259 T4121 T4119 pobj control,under
R2260 T4122 T4121 prep of,control
R2261 T4123 T4124 det the,elements
R2262 T4124 T4122 pobj elements,of
R2263 T4125 T4124 amod regulatory,elements
R2264 T4126 T4124 prep of,elements
R2265 T4127 T4128 det the,gene
R2266 T4128 T4126 pobj gene,of
R2267 T4129 T4128 nmod mouse,gene
R2268 T4130 T4128 nmod GATA,gene
R2269 T4131 T4130 punct -,GATA
R2270 T4132 T4130 nummod 1,GATA
R2271 T4133 T4128 punct (,gene
R2272 T4134 T4135 compound GATA1,cre
R2273 T4135 T4128 appos cre,gene
R2274 T4136 T4135 punct -,cre
R2275 T4137 T4094 punct ),crossed
R2276 T4138 T4139 punct [,14
R2277 T4139 T4094 parataxis 14,crossed
R2278 T4140 T4139 punct ],14
R2279 T4141 T4094 punct .,crossed
R2280 T4143 T4144 amod Widespread,expression
R2281 T4144 T4145 nsubjpass expression,demonstrated
R2282 T4146 T4144 prep of,expression
R2283 T4147 T4148 det the,transgene
R2284 T4148 T4146 pobj transgene,of
R2285 T4149 T4150 compound GATA1,cre
R2286 T4150 T4148 compound cre,transgene
R2287 T4151 T4150 punct -,cre
R2288 T4152 T4145 aux has,demonstrated
R2289 T4153 T4145 auxpass been,demonstrated
R2290 T4154 T4145 prep during,demonstrated
R2291 T4155 T4156 amod early,embryogenesis
R2292 T4156 T4154 pobj embryogenesis,during
R2293 T4157 T4158 punct [,14
R2294 T4158 T4145 parataxis 14,demonstrated
R2295 T4159 T4158 punct ],14
R2296 T4160 T4145 punct .,demonstrated
R2297 T4162 T4163 nsubj We,defined
R2298 T4164 T4165 advmod more,accurately
R2299 T4165 T4163 advmod accurately,defined
R2300 T4166 T4167 det the,onset
R2301 T4167 T4163 dobj onset,defined
R2302 T4168 T4167 prep of,onset
R2303 T4169 T4170 compound GATA1,cre
R2304 T4170 T4172 compound cre,expression
R2305 T4171 T4170 punct -,cre
R2306 T4172 T4168 pobj expression,of
R2307 T4173 T4163 advcl using,defined
R2308 T4174 T4175 det a,strain
R2309 T4175 T4173 dobj strain,using
R2310 T4176 T4175 compound ROSA26,strain
R2311 T4177 T4175 compound reporter,strain
R2312 T4178 T4175 punct ", ",strain
R2313 T4179 T4180 prep in,expressed
R2314 T4180 T4175 relcl expressed,strain
R2315 T4181 T4179 pobj which,in
R2316 T4182 T4183 det a,gene
R2317 T4183 T4180 nsubjpass gene,expressed
R2318 T4184 T4185 nmod β,galactosidase
R2319 T4185 T4187 nmod galactosidase,fusion
R2320 T4186 T4185 punct -,galactosidase
R2321 T4187 T4183 compound fusion,gene
R2322 T4188 T4185 punct /,galactosidase
R2323 T4189 T4185 appos neor,galactosidase
R2324 T4190 T4183 compound reporter,gene
R2325 T4191 T4180 auxpass is,expressed
R2326 T4192 T4193 advmod only,after
R2327 T4193 T4180 prep after,expressed
R2328 T4194 T4195 npadvmod Cre,mediated
R2329 T4195 T4197 amod mediated,excision
R2330 T4196 T4195 punct -,mediated
R2331 T4197 T4193 pobj excision,after
R2332 T4198 T4197 prep of,excision
R2333 T4199 T4200 npadvmod loxP,flanked
R2334 T4200 T4202 amod flanked,transcription
R2335 T4201 T4200 punct -,flanked
R2336 T4202 T4198 pobj transcription,of
R2337 T4203 T4197 cc and,excision
R2338 T4204 T4205 compound translation,termination
R2339 T4205 T4206 compound termination,signals
R2340 T4206 T4197 conj signals,excision
R2341 T4207 T4208 punct [,14
R2342 T4208 T4163 parataxis 14,defined
R2343 T4209 T4208 punct ],14
R2344 T4210 T4163 punct .,defined
R2345 T4212 T4213 nsubj We,found
R2346 T4214 T4215 mark that,was
R2347 T4215 T4213 ccomp was,found
R2348 T4216 T4217 det the,transgene
R2349 T4217 T4215 nsubj transgene,was
R2350 T4218 T4219 compound GATA1,cre
R2351 T4219 T4217 compound cre,transgene
R2352 T4220 T4219 punct -,cre
R2353 T4221 T4215 advmod already,was
R2354 T4222 T4215 acomp active,was
R2355 T4223 T4215 prep at,was
R2356 T4224 T4225 det the,stage
R2357 T4225 T4223 pobj stage,at
R2358 T4226 T4227 nummod 16,cell
R2359 T4227 T4225 compound cell,stage
R2360 T4228 T4227 punct -,cell
R2361 T4229 T4225 compound morula,stage
R2362 T4230 T4225 prep of,stage
R2363 T4231 T4230 pobj development,of
R2364 T4232 T4225 punct (,stage
R2365 T4233 T4234 nummod 0.5,days
R2366 T4234 T4235 npadvmod days,postcoitus
R2367 T4235 T4225 advmod postcoitus,stage
R2368 T4236 T4237 punct [,dpc
R2369 T4237 T4235 parataxis dpc,postcoitus
R2370 T4238 T4237 punct ],dpc
R2371 T4239 T4213 punct ),found
R2372 T4240 T4241 punct (,4A
R2373 T4241 T4213 parataxis 4A,found
R2374 T4242 T4241 compound Figure,4A
R2375 T4243 T4241 punct ),4A
R2376 T4244 T4213 punct .,found
R2377 T4246 T4247 aux To,generate
R2378 T4247 T4248 advcl generate,mated
R2379 T4249 T4250 amod Atrxnull,mice
R2380 T4250 T4247 dobj mice,generate
R2381 T4251 T4248 punct ", ",mated
R2382 T4252 T4253 amod heterozygous,females
R2383 T4253 T4248 nsubjpass females,mated
R2384 T4254 T4253 amod floxed,females
R2385 T4255 T4256 punct (,flox
R2386 T4256 T4253 parataxis flox,females
R2387 T4257 T4256 compound Atrx WT,flox
R2388 T4258 T4256 punct /,flox
R2389 T4259 T4256 punct ),flox
R2390 T4260 T4248 auxpass were,mated
R2391 T4261 T4248 prep with,mated
R2392 T4262 T4263 amod homozygous,males
R2393 T4263 T4261 pobj males,with
R2394 T4264 T4265 compound GATA1,cre
R2395 T4265 T4267 npadvmod cre,transgenic
R2396 T4266 T4265 punct -,cre
R2397 T4267 T4263 amod transgenic,males
R2398 T4268 T4269 punct (,cre
R2399 T4269 T4263 parataxis cre,males
R2400 T4270 T4271 compound Atrx WT,Y
R2401 T4271 T4269 dep Y,cre
R2402 T4272 T4271 punct /,Y
R2403 T4273 T4269 punct ;,cre
R2404 T4274 T4269 compound GATA1,cre
R2405 T4275 T4269 punct -,cre
R2406 T4276 T4269 punct +,cre
R2407 T4277 T4269 punct /,cre
R2408 T4278 T4269 punct +,cre
R2409 T4279 T4269 punct ),cre
R2410 T4280 T4248 punct .,mated
R2411 T4282 T4283 det No,males
R2412 T4283 T4285 nsubjpass males,recovered
R2413 T4284 T4283 amod Atrxnull,males
R2414 T4286 T4287 punct (,cre
R2415 T4287 T4283 parataxis cre,males
R2416 T4288 T4289 compound Atrx null,Y
R2417 T4289 T4287 dep Y,cre
R2418 T4290 T4289 punct /,Y
R2419 T4291 T4287 punct ;,cre
R2420 T4292 T4287 compound GATA1,cre
R2421 T4293 T4287 punct -,cre
R2422 T4294 T4287 punct +,cre
R2423 T4295 T4287 punct /,cre
R2424 T4296 T4287 punct −,cre
R2425 T4297 T4287 punct ),cre
R2426 T4298 T4285 auxpass were,recovered
R2427 T4299 T4285 prep at,recovered
R2428 T4300 T4299 pobj birth,at
R2429 T4301 T4285 punct ", ",recovered
R2430 T4302 T4285 advcl indicating,recovered
R2431 T4303 T4304 mark that,results
R2432 T4304 T4302 ccomp results,indicating
R2433 T4305 T4306 det the,absence
R2434 T4306 T4304 nsubj absence,results
R2435 T4307 T4306 prep of,absence
R2436 T4308 T4307 pobj Atrx,of
R2437 T4309 T4304 prep in,results
R2438 T4310 T4311 amod embryonic,lethality
R2439 T4311 T4309 pobj lethality,in
R2440 T4312 T4285 punct .,recovered
R2441 T4314 T4315 det This,finding
R2442 T4315 T4316 nsubj finding,was
R2443 T4317 T4316 acomp unexpected,was
R2444 T4318 T4316 punct ", ",was
R2445 T4319 T4320 mark since,survive
R2446 T4320 T4316 advcl survive,was
R2447 T4321 T4322 amod human,patients
R2448 T4322 T4320 nsubj patients,survive
R2449 T4323 T4324 compound ATR,X
R2450 T4324 T4322 compound X,patients
R2451 T4325 T4324 punct -,X
R2452 T4326 T4320 advmod clearly,survive
R2453 T4327 T4320 prep to,survive
R2454 T4328 T4327 pobj adulthood,to
R2455 T4329 T4330 punct (,see
R2456 T4330 T4320 parataxis see,survive
R2457 T4331 T4330 dobj Discussion,see
R2458 T4332 T4330 punct ),see
R2459 T4333 T4316 punct .,was
R2460 T4335 T4336 nsubjpass Embryos,dissected
R2461 T4337 T4336 auxpass were,dissected
R2462 T4338 T4336 prep at,dissected
R2463 T4339 T4340 nummod 7.5,dpc
R2464 T4340 T4338 pobj dpc,at
R2465 T4341 T4339 punct ", ",7.5
R2466 T4342 T4339 conj 8.5,7.5
R2467 T4343 T4342 punct ", ",8.5
R2468 T4344 T4342 cc and,8.5
R2469 T4345 T4342 conj 9.5,8.5
R2470 T4346 T4336 cc and,dissected
R2471 T4347 T4336 conj genotyped,dissected
R2472 T4348 T4347 prep by,genotyped
R2473 T4349 T4350 compound PCR,analysis
R2474 T4350 T4348 pobj analysis,by
R2475 T4351 T4350 prep of,analysis
R2476 T4352 T4351 pobj DNA,of
R2477 T4353 T4352 acl extracted,DNA
R2478 T4354 T4353 prep from,extracted
R2479 T4355 T4356 compound yolk,sac
R2480 T4356 T4354 pobj sac,from
R2481 T4357 T4356 cc or,sac
R2482 T4358 T4359 amod total,embryo
R2483 T4359 T4356 conj embryo,sac
R2484 T4360 T4361 punct (,4B
R2485 T4361 T4336 parataxis 4B,dissected
R2486 T4362 T4361 compound Figure,4B
R2487 T4363 T4361 cc and,4B
R2488 T4364 T4365 compound Protocol,S1
R2489 T4365 T4361 conj S1,4B
R249 T722 T723 compound ATR,X
R2490 T4366 T4361 punct ),4B
R2491 T4367 T4336 punct .,dissected
R2492 T4369 T4370 amod Atrxnull,males
R2493 T4370 T4371 nsubj males,were
R2494 T4372 T4371 acomp present,were
R2495 T4373 T4371 prep at,were
R2496 T4374 T4375 amod expected,ratios
R2497 T4375 T4373 pobj ratios,at
R2498 T4376 T4375 amod mendelian,ratios
R2499 T4377 T4378 punct (,%
R250 T723 T725 compound X,syndrome
R2500 T4378 T4375 parataxis %,ratios
R2501 T4379 T4380 punct ~,25
R2502 T4380 T4378 nummod 25,%
R2503 T4381 T4378 punct ),%
R2504 T4382 T4371 prep at,were
R2505 T4383 T4384 preconj both,dpc
R2506 T4384 T4382 pobj dpc,at
R2507 T4385 T4384 nummod 7.5,dpc
R2508 T4386 T4384 cc and,dpc
R2509 T4387 T4388 nummod 8.5,dpc
R251 T724 T723 punct -,X
R2510 T4388 T4384 conj dpc,dpc
R2511 T4389 T4390 punct (,Table
R2512 T4390 T4371 parataxis Table,were
R2513 T4391 T4390 nummod 1,Table
R2514 T4392 T4390 punct ),Table
R2515 T4393 T4371 punct .,were
R2516 T4395 T4396 advmod However,observed
R2517 T4397 T4396 punct ", ",observed
R2518 T4398 T4396 prep by,observed
R2519 T4399 T4400 nummod 9.5,dpc
R252 T725 T726 nsubj syndrome,is
R2520 T4400 T4398 pobj dpc,by
R2521 T4401 T4396 punct ", ",observed
R2522 T4402 T4396 nsubjpass depletion,observed
R2523 T4403 T4396 auxpass was,observed
R2524 T4404 T4405 preconj both,in
R2525 T4405 T4396 prep in,observed
R2526 T4406 T4407 det the,number
R2527 T4407 T4405 pobj number,in
R2528 T4408 T4407 prep of,number
R2529 T4409 T4410 amod Atrxnull,males
R253 T727 T728 det a,form
R2530 T4410 T4408 pobj males,of
R2531 T4411 T4412 punct (,%
R2532 T4412 T4407 parataxis %,number
R2533 T4413 T4412 nummod 7,%
R2534 T4414 T4412 punct ),%
R2535 T4415 T4405 cc and,in
R2536 T4416 T4405 conj in,in
R2537 T4417 T4418 det the,number
R2538 T4418 T4416 pobj number,in
R2539 T4419 T4418 amod total,number
R254 T728 T726 attr form,is
R2540 T4420 T4418 prep of,number
R2541 T4421 T4420 pobj males,of
R2542 T4422 T4421 acl recovered,males
R2543 T4423 T4424 punct (,%
R2544 T4424 T4418 parataxis %,number
R2545 T4425 T4424 nummod 31,%
R2546 T4426 T4424 punct ),%
R2547 T4427 T4396 punct .,observed
R2548 T4429 T4430 det No,males
R2549 T4430 T4432 nsubjpass males,recovered
R255 T729 T728 amod severe,form
R2550 T4431 T4430 amod Atrxnull,males
R2551 T4433 T4432 auxpass were,recovered
R2552 T4434 T4432 prep after,recovered
R2553 T4435 T4436 nummod 9.5,dpc
R2554 T4436 T4434 pobj dpc,after
R2555 T4437 T4432 punct .,recovered
R2556 T4439 T4440 advmod Thus,gives
R2557 T4441 T4442 det the,absence
R2558 T4442 T4440 nsubj absence,gives
R2559 T4443 T4442 prep of,absence
R256 T730 T728 punct ", ",form
R2560 T4444 T4443 pobj Atrx,of
R2561 T4445 T4440 dobj rise,gives
R2562 T4446 T4440 prep to,gives
R2563 T4447 T4448 amod embryonic,lethality
R2564 T4448 T4446 pobj lethality,to
R2565 T4449 T4440 prep in,gives
R2566 T4450 T4449 pobj mice,in
R2567 T4451 T4440 prep before,gives
R2568 T4452 T4453 nummod 9.5,dpc
R2569 T4453 T4451 pobj dpc,before
R257 T731 T728 amod nonprogressive,form
R2570 T4454 T4440 punct .,gives
R2571 T4456 T4457 aux Table 1 Distribution of Atrx Genotypes in Timed Matings To,investigate
R2572 T4457 T4458 advcl investigate,dissected
R2573 T4459 T4460 det the,morphology
R2574 T4460 T4457 dobj morphology,investigate
R2575 T4461 T4460 prep of,morphology
R2576 T4462 T4463 amod Atrxnull,embryos
R2577 T4463 T4461 pobj embryos,of
R2578 T4464 T4465 advmod prior,to
R2579 T4465 T4460 prep to,morphology
R258 T732 T728 prep of,form
R2580 T4466 T4465 pobj death,to
R2581 T4467 T4458 punct ", ",dissected
R2582 T4468 T4458 nsubjpass embryos,dissected
R2583 T4469 T4468 prep from,embryos
R2584 T4470 T4471 det the,crosses
R2585 T4471 T4469 pobj crosses,from
R2586 T4472 T4471 amod above,crosses
R2587 T4473 T4458 auxpass were,dissected
R2588 T4474 T4458 advmod initially,dissected
R2589 T4475 T4458 prep in,dissected
R259 T733 T734 npadvmod X,linked
R2590 T4476 T4477 poss their,deciduas
R2591 T4477 T4475 pobj deciduas,in
R2592 T4478 T4458 prep at,dissected
R2593 T4479 T4480 nummod 7.5,dpc
R2594 T4480 T4478 pobj dpc,at
R2595 T4481 T4458 punct ", ",dissected
R2596 T4482 T4458 cc and,dissected
R2597 T4483 T4484 compound paraffin,sections
R2598 T4484 T4485 nsubjpass sections,stained
R2599 T4485 T4458 conj stained,dissected
R260 T734 T736 amod linked,retardation
R2600 T4486 T4485 auxpass were,stained
R2601 T4487 T4485 prep with,stained
R2602 T4488 T4487 pobj haematoxylin,with
R2603 T4489 T4490 punct (,5A
R2604 T4490 T4487 parataxis 5A,with
R2605 T4491 T4490 compound Figure,5A
R2606 T4492 T4490 punct ),5A
R2607 T4493 T4487 cc or,with
R2608 T4494 T4487 conj with,with
R2609 T4495 T4496 det an,antibody
R261 T735 T734 punct -,linked
R2610 T4496 T4494 pobj antibody,with
R2611 T4497 T4496 amod anti-ATRX,antibody
R2612 T4498 T4499 punct (,5B
R2613 T4499 T4458 parataxis 5B,dissected
R2614 T4500 T4499 compound Figure,5B
R2615 T4501 T4502 punct –,5E
R2616 T4502 T4499 prep 5E,5B
R2617 T4503 T4499 punct ),5B
R2618 T4504 T4458 punct .,dissected
R2619 T4506 T4507 amod Immunohistochemical,staining
R262 T736 T732 pobj retardation,of
R2620 T4507 T4508 nsubj staining,revealed
R2621 T4509 T4510 mark that,expressed
R2622 T4510 T4508 ccomp expressed,revealed
R2623 T4511 T4510 nsubjpass Atrx,expressed
R2624 T4512 T4510 auxpass was,expressed
R2625 T4513 T4510 advmod widely,expressed
R2626 T4514 T4510 prep in,expressed
R2627 T4515 T4516 amod wild,type
R2628 T4516 T4518 nmod type,embryos
R2629 T4517 T4516 punct -,type
R263 T737 T736 amod mental,retardation
R2630 T4518 T4514 pobj embryos,in
R2631 T4519 T4520 nummod 7.5,dpc
R2632 T4520 T4518 compound dpc,embryos
R2633 T4521 T4522 punct (,5B
R2634 T4522 T4508 parataxis 5B,revealed
R2635 T4523 T4522 compound Figure,5B
R2636 T4524 T4522 punct ),5B
R2637 T4525 T4508 punct .,revealed
R2638 T4527 T4528 nsubj Expression,was
R2639 T4529 T4528 acomp highest,was
R264 T738 T739 dep that,associated
R2640 T4530 T4528 prep in,was
R2641 T4531 T4532 det the,region
R2642 T4532 T4530 pobj region,in
R2643 T4533 T4532 amod embryonic,region
R2644 T4534 T4535 punct (,5C
R2645 T4535 T4532 parataxis 5C,region
R2646 T4536 T4535 compound Figure,5C
R2647 T4537 T4535 punct ),5C
R2648 T4538 T4532 cc and,region
R2649 T4539 T4540 det the,chorion
R265 T739 T728 relcl associated,form
R2650 T4540 T4532 conj chorion,region
R2651 T4541 T4542 punct (,5D
R2652 T4542 T4540 parataxis 5D,chorion
R2653 T4543 T4542 compound Figure,5D
R2654 T4544 T4542 punct ),5D
R2655 T4545 T4528 punct .,was
R2656 T4547 T4548 amod Detectable,levels
R2657 T4548 T4551 nsubjpass levels,observed
R2658 T4549 T4547 cc but,Detectable
R2659 T4550 T4547 conj lower,Detectable
R266 T740 T739 auxpass is,associated
R2660 T4552 T4548 prep of,levels
R2661 T4553 T4552 pobj expression,of
R2662 T4554 T4551 auxpass were,observed
R2663 T4555 T4551 prep in,observed
R2664 T4556 T4557 det the,cone
R2665 T4557 T4555 pobj cone,in
R2666 T4558 T4557 amod ectoplacental,cone
R2667 T4559 T4560 punct (,5D
R2668 T4560 T4557 parataxis 5D,cone
R2669 T4561 T4560 compound Figure,5D
R267 T741 T739 advmod frequently,associated
R2670 T4562 T4560 punct ),5D
R2671 T4563 T4557 cc and,cone
R2672 T4564 T4565 amod surrounding,tissue
R2673 T4565 T4557 conj tissue,cone
R2674 T4566 T4565 amod decidual,tissue
R2675 T4567 T4551 punct .,observed
R2676 T4569 T4570 nsubj We,observed
R2677 T4571 T4570 advmod also,observed
R2678 T4572 T4573 advmod very,high
R2679 T4573 T4574 amod high,levels
R268 T742 T739 prep with,associated
R2680 T4574 T4570 dobj levels,observed
R2681 T4575 T4574 prep of,levels
R2682 T4576 T4577 compound Atrx,expression
R2683 T4577 T4575 pobj expression,of
R2684 T4578 T4570 prep in,observed
R2685 T4579 T4580 nmod trophoblast,cells
R2686 T4580 T4578 pobj cells,in
R2687 T4581 T4580 amod giant,cells
R2688 T4582 T4580 punct (,cells
R2689 T4583 T4580 appos TGCs,cells
R269 T743 T744 amod multiple,abnormalities
R2690 T4584 T4580 punct ),cells
R2691 T4585 T4580 acl surrounding,cells
R2692 T4586 T4587 det the,membrane
R2693 T4587 T4585 dobj membrane,surrounding
R2694 T4588 T4587 poss Reichert,membrane
R2695 T4589 T4588 case 's,Reichert
R2696 T4590 T4591 punct (,5E
R2697 T4591 T4570 parataxis 5E,observed
R2698 T4592 T4591 compound Figure,5E
R2699 T4593 T4591 punct ),5E
R270 T744 T742 pobj abnormalities,with
R2700 T4594 T4570 punct .,observed
R2701 T4596 T4597 prep Within,was
R2702 T4598 T4599 det the,nuclei
R2703 T4599 T4596 pobj nuclei,Within
R2704 T4600 T4599 amod large,nuclei
R2705 T4601 T4599 prep of,nuclei
R2706 T4602 T4603 det these,TGCs
R2707 T4603 T4601 pobj TGCs,of
R2708 T4604 T4597 punct ", ",was
R2709 T4605 T4606 det the,association
R271 T745 T744 amod congenital,abnormalities
R2710 T4606 T4597 nsubj association,was
R2711 T4607 T4606 amod typical,association
R2712 T4608 T4606 amod nuclear,association
R2713 T4609 T4606 prep of,association
R2714 T4610 T4609 pobj Atrx,of
R2715 T4611 T4606 prep with,association
R2716 T4612 T4611 pobj blocks,with
R2717 T4613 T4612 prep of,blocks
R2718 T4614 T4615 amod pericentromeric,heterochromatin
R2719 T4615 T4613 pobj heterochromatin,of
R272 T746 T747 punct [,1
R2720 T4616 T4617 punct [,15
R2721 T4617 T4606 parataxis 15,association
R2722 T4618 T4617 punct ],15
R2723 T4619 T4620 advmod clearly,observable
R2724 T4620 T4597 acomp observable,was
R2725 T4621 T4597 punct .,was
R2726 T4623 T4624 advmod Only,staining
R2727 T4624 T4626 nsubjpass staining,seen
R2728 T4625 T4624 compound background,staining
R2729 T4627 T4626 auxpass was,seen
R273 T747 T726 parataxis 1,is
R2730 T4628 T4626 prep in,seen
R2731 T4629 T4630 det the,tissues
R2732 T4630 T4628 pobj tissues,in
R2733 T4631 T4630 amod corresponding,tissues
R2734 T4632 T4630 amod Atrxnull,tissues
R2735 T4633 T4630 amod embryonic,tissues
R2736 T4634 T4635 punct (,5B
R2737 T4635 T4626 parataxis 5B,seen
R2738 T4636 T4635 compound Figure,5B
R2739 T4637 T4638 punct –,5D
R274 T748 T747 punct ],1
R2740 T4638 T4635 prep 5D,5B
R2741 T4639 T4635 punct ),5B
R2742 T4640 T4626 punct ", ",seen
R2743 T4641 T4642 mark while,was
R2744 T4642 T4626 advcl was,seen
R2745 T4643 T4642 nsubj expression,was
R2746 T4644 T4643 prep in,expression
R2747 T4645 T4646 det the,tissue
R2748 T4646 T4644 pobj tissue,in
R2749 T4647 T4646 amod surrounding,tissue
R275 T749 T726 punct .,is
R2750 T4648 T4646 amod decidual,tissue
R2751 T4649 T4646 punct (,tissue
R2752 T4650 T4646 prep of,tissue
R2753 T4651 T4652 amod maternal,origin
R2754 T4652 T4650 pobj origin,of
R2755 T4653 T4646 punct ),tissue
R2756 T4654 T4642 acomp normal,was
R2757 T4655 T4642 cc and,was
R2758 T4656 T4642 conj served,was
R2759 T4657 T4656 prep as,served
R276 T751 T752 nsubjpass It,associated
R2760 T4658 T4659 det an,control
R2761 T4659 T4657 pobj control,as
R2762 T4660 T4659 compound antibody,control
R2763 T4661 T4659 compound staining,control
R2764 T4662 T4663 punct (,data
R2765 T4663 T4656 meta data,served
R2766 T4664 T4663 amod unpublished,data
R2767 T4665 T4663 punct ),data
R2768 T4666 T4626 punct .,seen
R2769 T4668 T4669 advmod Morphologically,reduced
R277 T753 T752 auxpass is,associated
R2770 T4670 T4669 punct ", ",reduced
R2771 T4671 T4672 nummod 7.5,dpc
R2772 T4672 T4673 nmod dpc,embryos
R2773 T4673 T4669 nsubjpass embryos,reduced
R2774 T4674 T4673 amod Atrxnull,embryos
R2775 T4675 T4669 auxpass were,reduced
R2776 T4676 T4669 advmod dramatically,reduced
R2777 T4677 T4669 prep in,reduced
R2778 T4678 T4677 pobj size,in
R2779 T4679 T4669 cc and,reduced
R278 T754 T752 advmod usually,associated
R2780 T4680 T4669 conj appeared,reduced
R2781 T4681 T4682 advmod developmentally,retarded
R2782 T4682 T4680 oprd retarded,appeared
R2783 T4683 T4680 advcl relative,appeared
R2784 T4684 T4683 prep to,relative
R2785 T4685 T4686 npadvmod stage,matched
R2786 T4686 T4688 amod matched,embryos
R2787 T4687 T4686 punct -,matched
R2788 T4688 T4684 pobj embryos,to
R2789 T4689 T4690 amod wild,type
R279 T755 T752 prep with,associated
R2790 T4690 T4688 compound type,embryos
R2791 T4691 T4690 punct -,type
R2792 T4692 T4693 punct (,5A
R2793 T4693 T4680 parataxis 5A,appeared
R2794 T4694 T4693 compound Figure,5A
R2795 T4695 T4693 cc and,5A
R2796 T4696 T4693 conj 5B,5A
R2797 T4697 T4693 punct ),5A
R2798 T4698 T4669 punct .,reduced
R2799 T4700 T4701 advmod However,appeared
R280 T756 T757 det a,form
R2800 T4702 T4701 punct ", ",appeared
R2801 T4703 T4701 prep despite,appeared
R2802 T4704 T4705 poss their,size
R2803 T4705 T4703 pobj size,despite
R2804 T4706 T4705 amod reduced,size
R2805 T4707 T4701 punct ", ",appeared
R2806 T4708 T4709 det the,morphology
R2807 T4709 T4701 nsubj morphology,appeared
R2808 T4710 T4709 amod general,morphology
R2809 T4711 T4709 cc and,morphology
R281 T757 T755 pobj form,with
R2810 T4712 T4709 conj organisation,morphology
R2811 T4713 T4709 prep of,morphology
R2812 T4714 T4715 amod embryonic,structures
R2813 T4715 T4713 pobj structures,of
R2814 T4716 T4709 prep in,morphology
R2815 T4717 T4718 amod Atrxnull,conceptuses
R2816 T4718 T4716 pobj conceptuses,in
R2817 T4719 T4720 advmod grossly,normal
R2818 T4720 T4701 oprd normal,appeared
R2819 T4721 T4701 punct .,appeared
R282 T758 T757 amod mild,form
R2820 T4723 T4724 det The,amnion
R2821 T4724 T4725 nsubj amnion,were
R2822 T4726 T4724 cc and,amnion
R2823 T4727 T4724 conj chorion,amnion
R2824 T4728 T4729 advmod clearly,present
R2825 T4729 T4725 acomp present,were
R2826 T4730 T4725 cc and,were
R2827 T4731 T4732 det the,cavities
R2828 T4732 T4739 nsubj cavities,were
R2829 T4733 T4732 amod amniotic,cavities
R283 T759 T757 prep of,form
R2830 T4734 T4733 punct ", ",amniotic
R2831 T4735 T4733 conj exocoelomic,amniotic
R2832 T4736 T4735 punct ", ",exocoelomic
R2833 T4737 T4735 cc and,exocoelomic
R2834 T4738 T4735 conj ectoplacental,exocoelomic
R2835 T4739 T4725 conj were,were
R2836 T4740 T4739 acomp distinguishable,were
R2837 T4741 T4739 punct ", ",were
R2838 T4742 T4743 mark as,were
R2839 T4743 T4739 advcl were,were
R284 T760 T761 compound α,thalassaemia
R2840 T4744 T4745 det all,layers
R2841 T4745 T4743 nsubj layers,were
R2842 T4746 T4745 nummod three,layers
R2843 T4747 T4745 amod embryonic,layers
R2844 T4748 T4745 compound germ,layers
R2845 T4749 T4750 punct (,5A
R2846 T4750 T4739 parataxis 5A,were
R2847 T4751 T4750 compound Figure,5A
R2848 T4752 T4753 punct –,5C
R2849 T4753 T4750 prep 5C,5A
R285 T761 T759 pobj thalassaemia,of
R2850 T4754 T4750 punct ),5A
R2851 T4755 T4739 punct .,were
R2852 T4757 T4758 prep At,dissected
R2853 T4759 T4760 nummod 8.5,dpc
R2854 T4760 T4757 pobj dpc,At
R2855 T4761 T4758 punct ", ",dissected
R2856 T4762 T4758 nsubjpass embryos,dissected
R2857 T4763 T4758 auxpass were,dissected
R2858 T4764 T4758 advcl free,dissected
R2859 T4765 T4764 prep of,free
R286 T762 T761 punct -,thalassaemia
R2860 T4766 T4765 pobj deciduas,of
R2861 T4767 T4758 punct ", ",dissected
R2862 T4768 T4758 cc and,dissected
R2863 T4769 T4758 conj observed,dissected
R2864 T4770 T4769 prep in,observed
R2865 T4771 T4772 amod whole,mount
R2866 T4772 T4770 pobj mount,in
R2867 T4773 T4758 punct .,dissected
R2868 T4775 T4776 amod Individual,conceptuses
R2869 T4776 T4777 nsubjpass conceptuses,genotyped
R287 T763 T757 punct ", ",form
R2870 T4778 T4777 auxpass were,genotyped
R2871 T4779 T4777 prep by,genotyped
R2872 T4780 T4779 pobj PCR,by
R2873 T4781 T4777 advcl using,genotyped
R2874 T4782 T4781 dobj DNA,using
R2875 T4783 T4782 acl isolated,DNA
R2876 T4784 T4783 prep from,isolated
R2877 T4785 T4786 compound yolk,sac
R2878 T4786 T4784 pobj sac,from
R2879 T4787 T4788 mark as,described
R288 T764 T757 acl caused,form
R2880 T4788 T4777 advcl described,genotyped
R2881 T4789 T4788 prep in,described
R2882 T4790 T4791 compound Protocol,S1
R2883 T4791 T4789 pobj S1,in
R2884 T4792 T4777 punct .,genotyped
R2885 T4794 T4795 advcl Consistent,appeared
R2886 T4796 T4794 prep with,Consistent
R2887 T4797 T4796 pobj observations,with
R2888 T4798 T4797 prep at,observations
R2889 T4799 T4800 nummod 7.5,dpc
R289 T765 T764 agent by,caused
R2890 T4800 T4798 pobj dpc,at
R2891 T4801 T4795 punct ", ",appeared
R2892 T4802 T4803 det the,morphology
R2893 T4803 T4795 nsubj morphology,appeared
R2894 T4804 T4803 amod general,morphology
R2895 T4805 T4803 prep of,morphology
R2896 T4806 T4807 det the,embryo
R2897 T4807 T4805 pobj embryo,of
R2898 T4808 T4807 amod proper,embryo
R2899 T4809 T4807 prep of,embryo
R290 T766 T767 amod reduced,expression
R2900 T4810 T4811 amod Atrxnull,conceptuses
R2901 T4811 T4809 pobj conceptuses,of
R2902 T4812 T4795 advmod also,appeared
R2903 T4813 T4814 advmod grossly,normal
R2904 T4814 T4795 oprd normal,appeared
R2905 T4815 T4795 prep at,appeared
R2906 T4816 T4817 nummod 8.5,dpc
R2907 T4817 T4815 pobj dpc,at
R2908 T4818 T4795 punct .,appeared
R2909 T4820 T4821 det The,fold
R291 T767 T765 pobj expression,by
R2910 T4821 T4823 nsubj fold,formed
R2911 T4822 T4821 compound head,fold
R2912 T4824 T4823 aux had,formed
R2913 T4825 T4823 advmod clearly,formed
R2914 T4826 T4823 punct ", ",formed
R2915 T4827 T4823 cc and,formed
R2916 T4828 T4829 nsubjpass expression,detected
R2917 T4829 T4823 conj detected,formed
R2918 T4830 T4828 prep of,expression
R2919 T4831 T4832 det the,brachyury
R292 T768 T767 prep of,expression
R2920 T4832 T4830 pobj brachyury,of
R2921 T4833 T4832 amod early,brachyury
R2922 T4834 T4832 compound mesoderm,brachyury
R2923 T4835 T4832 compound marker,brachyury
R2924 T4836 T4837 punct (,T
R2925 T4837 T4832 appos T,brachyury
R2926 T4838 T4828 punct ),expression
R2927 T4839 T4840 punct [,16
R2928 T4840 T4828 parataxis 16,expression
R2929 T4841 T4840 punct ],16
R293 T769 T770 advmod structurally,intact
R2930 T4842 T4829 auxpass was,detected
R2931 T4843 T4829 prep in,detected
R2932 T4844 T4845 det the,streak
R2933 T4845 T4843 pobj streak,in
R2934 T4846 T4845 amod primitive,streak
R2935 T4847 T4845 cc and,streak
R2936 T4848 T4849 amod emerging,notochord
R2937 T4849 T4845 conj notochord,streak
R2938 T4850 T4829 prep by,detected
R2939 T4851 T4852 amod whole,mount
R294 T770 T771 amod intact,genes
R2940 T4852 T4854 nmod mount,hybridisation
R2941 T4853 T4852 punct -,mount
R2942 T4854 T4850 pobj hybridisation,by
R2943 T4855 T4856 advmod in,situ
R2944 T4856 T4854 amod situ,hybridisation
R2945 T4857 T4854 punct (,hybridisation
R2946 T4858 T4854 appos WMISH,hybridisation
R2947 T4859 T4829 punct ),detected
R2948 T4860 T4861 punct (,5F
R2949 T4861 T4829 parataxis 5F,detected
R295 T771 T768 pobj genes,of
R2950 T4862 T4861 compound Figure,5F
R2951 T4863 T4861 punct ),5F
R2952 T4864 T4829 punct ", ",detected
R2953 T4865 T4829 advcl indicating,detected
R2954 T4866 T4867 mark that,gastrulated
R2955 T4867 T4865 ccomp gastrulated,indicating
R2956 T4868 T4869 amod Atrxnull,embryos
R2957 T4869 T4867 nsubj embryos,gastrulated
R2958 T4870 T4867 aux had,gastrulated
R2959 T4871 T4829 punct .,detected
R296 T772 T773 compound α,globin
R2960 T4873 T4874 aux To,investigate
R2961 T4874 T4875 advcl investigate,analysed
R2962 T4876 T4877 mark whether,was
R2963 T4877 T4874 ccomp was,investigate
R2964 T4878 T4879 det the,size
R2965 T4879 T4877 nsubj size,was
R2966 T4880 T4879 amod reduced,size
R2967 T4881 T4879 prep of,size
R2968 T4882 T4883 det the,embryos
R2969 T4883 T4881 pobj embryos,of
R297 T773 T771 compound globin,genes
R2970 T4884 T4883 amod Atrxnull,embryos
R2971 T4885 T4877 prep due,was
R2972 T4886 T4885 pcomp to,due
R2973 T4887 T4888 det an,increase
R2974 T4888 T4885 pobj increase,due
R2975 T4889 T4888 prep in,increase
R2976 T4890 T4889 pobj apoptosis,in
R2977 T4891 T4875 punct ", ",analysed
R2978 T4892 T4875 nsubj we,analysed
R2979 T4893 T4875 dobj sections,analysed
R298 T774 T773 punct -,globin
R2980 T4894 T4893 prep of,sections
R2981 T4895 T4896 npadvmod paraffin,embedded
R2982 T4896 T4898 amod embedded,embryos
R2983 T4897 T4896 punct -,embedded
R2984 T4898 T4894 pobj embryos,of
R2985 T4899 T4900 nummod 7.5,dpc
R2986 T4900 T4898 compound dpc,embryos
R2987 T4901 T4875 prep by,analysed
R2988 T4902 T4903 npadvmod TdT,mediated
R2989 T4903 T4905 amod mediated,labeling
R299 T775 T752 punct ", ",associated
R2990 T4904 T4903 punct -,mediated
R2991 T4905 T4909 nmod labeling,assay
R2992 T4906 T4905 nmod dUTP,labeling
R2993 T4907 T4905 nmod nick,labeling
R2994 T5014 T5013 prep in,observed
R2995 T5015 T5016 amod Atrxnull,embryos
R2996 T5016 T5014 pobj embryos,in
R2997 T5017 T5003 punct ", ",investigated
R2998 T5018 T5019 det a,defect
R2999 T5019 T5003 nsubjpass defect,investigated
R300 T776 T752 cc and,associated
R3000 T5020 T5019 amod possible,defect
R3001 T5021 T5019 compound proliferation,defect
R3002 T5022 T5003 auxpass was,investigated
R3003 T5023 T5003 advmod also,investigated
R3004 T5024 T5003 prep by,investigated
R3005 T5025 T5026 amod immunohistochemical,staining
R3006 T5026 T5024 pobj staining,by
R3007 T5027 T5026 prep of,staining
R3008 T5028 T5029 nummod 7.5,dpc
R3009 T5029 T5030 compound dpc,embryo
R301 T777 T752 conj characterised,associated
R3010 T5030 T5031 compound embryo,sections
R3011 T5031 T5027 pobj sections,of
R3012 T5032 T5003 prep for,investigated
R3013 T5033 T5034 det the,H3
R3014 T5034 T5032 pobj H3,for
R3015 T5035 T5036 compound mitosis,marker
R3016 T4908 T4905 nmod end,labeling
R3017 T5036 T5037 npadvmod marker,phosphorylated
R3018 T4909 T4901 pobj assay,by
R3019 T4910 T4905 punct (,labeling
R302 T778 T777 prep by,characterised
R3020 T5037 T5034 amod phosphorylated,H3
R3021 T4911 T4905 appos TUNEL,labeling
R3022 T4912 T4909 punct ),assay
R3023 T5038 T5039 punct (,Ser10
R3024 T4913 T4914 punct (,6A
R3025 T4914 T4875 parataxis 6A,analysed
R3026 T4915 T4914 compound Figure,6A
R3027 T5039 T5037 parataxis Ser10,phosphorylated
R3028 T4916 T4914 punct ),6A
R3029 T4917 T4875 punct .,analysed
R303 T779 T780 det the,presence
R3030 T4919 T4920 advmod Very,few
R3031 T4920 T4921 amod few,cells
R3032 T5040 T5039 punct ),Ser10
R3033 T4921 T4923 nsubjpass cells,detected
R3034 T4922 T4921 amod apoptotic,cells
R3035 T5041 T5034 compound histone,H3
R3036 T4924 T4923 auxpass were,detected
R3037 T4925 T4923 prep in,detected
R3038 T5042 T5043 punct [,13
R3039 T4926 T4927 amod wild,type
R304 T780 T778 pobj presence,by
R3040 T4927 T4929 nmod type,embryos
R3041 T4928 T4927 punct -,type
R3042 T5043 T5003 parataxis 13,investigated
R3043 T4929 T4925 pobj embryos,in
R3044 T4930 T4931 nummod 7.5,dpc
R3045 T4931 T4929 compound dpc,embryos
R3046 T5044 T5043 punct ],13
R3047 T4932 T4923 punct .,detected
R3048 T5045 T5003 punct .,investigated
R3049 T4934 T4935 prep In,was
R305 T781 T780 prep of,presence
R3050 T4936 T4937 amod Atrxnull,embryos
R3051 T5047 T5048 advcl Relative,reduced
R3052 T4937 T4934 pobj embryos,In
R3053 T4938 T4935 punct ", ",was
R3054 T4939 T4940 det a,increase
R3055 T5049 T5047 prep to,Relative
R3056 T4940 T4935 nsubj increase,was
R3057 T4941 T4940 amod slight,increase
R3058 T4942 T4940 prep in,increase
R3059 T4943 T4944 det the,population
R306 T782 T783 compound β,globin
R3060 T4944 T4942 pobj population,in
R3061 T5050 T5051 det the,index
R3062 T4945 T4944 amod apoptotic,population
R3063 T4946 T4935 acomp evident,was
R3064 T4947 T4935 punct .,was
R3065 T5051 T5049 pobj index,to
R3066 T4949 T4950 advmod However,was
R3067 T4951 T4950 punct ", ",was
R3068 T5052 T5053 advmod very,high
R3069 T4952 T4950 advcl consistent,was
R307 T783 T785 compound globin,tetramers
R3070 T4953 T4952 prep with,consistent
R3071 T4954 T4955 poss our,observation
R3072 T5053 T5051 amod high,index
R3073 T4955 T4953 pobj observation,with
R3074 T4956 T4955 prep of,observation
R3075 T4957 T4958 det a,index
R3076 T5054 T5051 amod mitotic,index
R3077 T4958 T4956 pobj index,of
R3078 T4959 T4960 advmod grossly,normal
R3079 T4960 T4958 amod normal,index
R308 T784 T783 punct -,globin
R3080 T5055 T5051 acl observed,index
R3081 T4961 T4958 amod apoptotic,index
R3082 T4962 T4955 prep in,observation
R3083 T4963 T4964 amod Atrxnull,ES
R3084 T4964 T4965 compound ES,cells
R3085 T4965 T4962 pobj cells,in
R3086 T5056 T5055 prep in,observed
R3087 T4966 T4967 punct (,S2
R3088 T5057 T5058 amod wild,type
R3089 T4967 T4952 parataxis S2,consistent
R309 T785 T781 pobj tetramers,of
R3090 T5058 T5060 compound type,embryos
R3091 T4968 T4967 compound Figure,S2
R3092 T4969 T4967 punct ),S2
R3093 T4970 T4950 punct ", ",was
R3094 T5059 T5058 punct -,type
R3095 T4971 T4972 det the,response
R3096 T4972 T4950 nsubj response,was
R3097 T5060 T5056 pobj embryos,in
R3098 T4973 T4972 amod apoptotic,response
R3099 T4974 T4972 acl observed,response
R310 T786 T785 punct (,tetramers
R3100 T4975 T4974 prep in,observed
R3101 T5061 T5048 punct ", ",reduced
R3102 T4976 T4977 amod Atrxnull,embryos
R3103 T4977 T4975 pobj embryos,in
R3104 T5062 T5063 det the,proportion
R3105 T4978 T4950 advmod also,was
R3106 T4979 T4950 neg not,was
R3107 T4980 T4950 acomp uniform,was
R3108 T5063 T5048 nsubjpass proportion,reduced
R3109 T4981 T4950 punct ", ",was
R311 T787 T788 compound haemoglobin,H
R3110 T4982 T4950 cc but,was
R3111 T5064 T5063 prep of,proportion
R3112 T4983 T4984 auxpass was,restricted
R3113 T4984 T4950 conj restricted,was
R3114 T4985 T4984 prep to,restricted
R3115 T5065 T5066 amod mitotic,cells
R3116 T4986 T4987 det a,number
R3117 T4987 T4985 pobj number,to
R3118 T4988 T4987 amod low,number
R3119 T5066 T5064 pobj cells,of
R312 T788 T789 compound H,bodies
R3120 T4989 T4987 prep of,number
R3121 T4990 T4991 amod scattered,cells
R3122 T4991 T4989 pobj cells,of
R3123 T5067 T5066 acl observed,cells
R3124 T4992 T4993 npadvmod TUNEL,positive
R3125 T4993 T4991 amod positive,cells
R3126 T4994 T4993 punct -,positive
R3127 T5068 T5067 prep in,observed
R3128 T4995 T4950 punct .,was
R3129 T5069 T5070 amod Atrxnull,embryos
R313 T789 T785 appos bodies,tetramers
R3130 T4997 T4998 mark Since,is
R3131 T4998 T5003 advcl is,investigated
R3132 T4999 T5000 det this,response
R3133 T5070 T5068 pobj embryos,in
R3134 T5000 T4998 nsubj response,is
R3135 T5001 T5000 amod small,response
R3136 T5002 T5000 amod apoptotic,response
R3137 T5004 T4998 acomp unlikely,is
R3138 T5005 T5006 aux to,account
R3139 T5071 T5067 prep at,observed
R314 T790 T789 compound inclusion,bodies
R3140 T5006 T5004 xcomp account,unlikely
R3141 T5007 T5006 advmod fully,account
R3142 T5008 T5006 prep for,account
R3143 T5072 T5073 nummod 7.5,dpc
R3144 T5009 T5010 det the,deficit
R3145 T5010 T5008 pobj deficit,for
R3146 T5073 T5071 pobj dpc,at
R3147 T5011 T5010 amod dramatic,deficit
R3148 T5012 T5010 compound size,deficit
R3149 T5013 T5010 acl observed,deficit
R315 T791 T780 punct ),presence
R3150 T5074 T5048 auxpass was,reduced
R3151 T5075 T5048 advmod dramatically,reduced
R3152 T5076 T5077 punct (,6B
R3153 T5077 T5048 parataxis 6B,reduced
R3154 T5078 T5077 compound Figure,6B
R3155 T5079 T5077 punct ),6B
R3156 T5080 T5048 punct .,reduced
R3157 T5120 T5119 compound growth,defect
R3158 T5082 T5083 advcl Taken,suggest
R3159 T5121 T5117 auxpass was,observed
R316 T792 T780 prep in,presence
R3160 T5122 T5117 advmod also,observed
R3161 T5084 T5082 advmod together,Taken
R3162 T5124 T5117 prep in,observed
R3163 T5125 T5126 amod Atrxnull,cells
R3164 T5126 T5124 pobj cells,in
R3165 T5085 T5083 punct ", ",suggest
R3166 T5127 T5126 compound ES,cells
R3167 T5128 T5129 punct (,3A
R3168 T5129 T5117 parataxis 3A,observed
R3169 T5086 T5087 det these,results
R317 T793 T794 amod peripheral,cells
R3170 T5130 T5129 compound Figure,3A
R3171 T5131 T5129 punct ),3A
R3172 T5087 T5083 nsubj results,suggest
R3173 T5132 T5123 punct ", ",depleted
R3174 T5133 T5123 prep in,depleted
R3175 T5134 T5133 pobj contrast,in
R3176 T5088 T5089 mark that,reflects
R3177 T5135 T5134 prep to,contrast
R3178 T5136 T5137 det the,embryos
R3179 T5089 T5083 ccomp reflects,suggest
R318 T794 T792 pobj cells,in
R3180 T5090 T5091 det the,deficit
R3181 T5137 T5135 pobj embryos,to
R3182 T5091 T5089 nsubj deficit,reflects
R3183 T5138 T5137 amod Atrxnull,embryos
R3184 T5139 T5123 punct ", ",depleted
R3185 T5140 T5141 det the,index
R3186 T5141 T5123 nsubjpass index,depleted
R3187 T5092 T5091 compound size,deficit
R3188 T5142 T5141 amod mitotic,index
R3189 T5143 T5141 prep of,index
R319 T795 T794 amod red,cells
R3190 T5144 T5145 det the,population
R3191 T5093 T5091 acl observed,deficit
R3192 T5145 T5143 pobj population,of
R3193 T5146 T5147 compound ES,cell
R3194 T5094 T5093 prep in,observed
R3195 T5147 T5145 compound cell,population
R3196 T5148 T5141 punct (,index
R3197 T5095 T5096 amod Atrxnull,embryos
R3198 T5149 T5150 mark as,measured
R3199 T5150 T5141 advcl measured,index
R320 T796 T794 compound blood,cells
R3200 T5151 T5150 prep with,measured
R3201 T5096 T5094 pobj embryos,in
R3202 T5152 T5153 det the,antibody
R3203 T5153 T5151 pobj antibody,with
R3204 T5097 T5098 amod prior,to
R3205 T5154 T5153 amod same,antibody
R3206 T5155 T5123 punct ),depleted
R3207 T5156 T5123 auxpass was,depleted
R3208 T5098 T5093 prep to,observed
R3209 T5157 T5123 neg not,depleted
R321 T797 T752 punct .,associated
R3210 T5158 T5159 punct (,S1B
R3211 T5099 T5098 pobj lethality,to
R3212 T5159 T5123 parataxis S1B,depleted
R3213 T5160 T5159 compound Figure,S1B
R3214 T5161 T5159 punct ),S1B
R3215 T5100 T5089 advmod largely,reflects
R3216 T5162 T5123 punct .,depleted
R3217 T5101 T5102 det a,defect
R3218 T5164 T5165 det These,observations
R3219 T5165 T5166 nsubj observations,suggest
R322 T799 T800 compound Carrier,females
R3220 T5167 T5168 mark that,is
R3221 T5102 T5089 dobj defect,reflects
R3222 T5168 T5166 ccomp is,suggest
R3223 T5169 T5170 det the,defect
R3224 T5170 T5168 nsubj defect,is
R3225 T5103 T5102 amod proliferative,defect
R3226 T5171 T5170 amod mitotic,defect
R3227 T5172 T5170 acl observed,defect
R3228 T5104 T5089 punct ", ",reflects
R3229 T5173 T5172 prep in,observed
R323 T800 T801 nsubj females,manifest
R3230 T5174 T5173 pobj embryos,in
R3231 T5175 T5168 acomp unlikely,is
R3232 T5105 T5089 prep with,reflects
R3233 T5176 T5177 aux to,be
R3234 T5177 T5175 xcomp be,unlikely
R3235 T5106 T5107 det a,contribution
R3236 T5178 T5179 det a,effect
R3237 T5179 T5177 attr effect,be
R3238 T5180 T5179 amod direct,effect
R3239 T5181 T5179 punct ", ",effect
R324 T802 T801 advmod occasionally,manifest
R3240 T5182 T5183 npadvmod cell,autonomous
R3241 T5107 T5105 pobj contribution,with
R3242 T5183 T5179 amod autonomous,effect
R3243 T5184 T5183 punct -,autonomous
R3244 T5185 T5179 prep of,effect
R3245 T5186 T5187 det the,absence
R3246 T5108 T5107 amod minor,contribution
R3247 T5187 T5185 pobj absence,of
R3248 T5188 T5187 prep of,absence
R3249 T5109 T5108 cc but,minor
R325 T803 T804 compound haemoglobin,H
R3250 T5189 T5188 pobj Atrx,of
R3251 T5190 T5168 punct ", ",is
R3252 T5110 T5108 conj indirect,minor
R3253 T5191 T5168 cc and,is
R3254 T5192 T5168 conj is,is
R3255 T5193 T5194 advmod more,likely
R3256 T5111 T5107 prep from,contribution
R3257 T5194 T5192 acomp likely,is
R3258 T5112 T5113 amod increased,apoptosis
R3259 T5195 T5196 aux to,be
R326 T804 T805 compound H,inclusions
R3260 T5113 T5111 pobj apoptosis,from
R3261 T5196 T5194 xcomp be,likely
R3262 T5197 T5198 det a,effect
R3263 T5198 T5196 attr effect,be
R3264 T5114 T5083 punct .,suggest
R3265 T5199 T5198 amod secondary,effect
R3266 T5200 T5198 acl resulting,effect
R3267 T5116 T5117 mark Although,observed
R3268 T5201 T5200 prep from,resulting
R3269 T5202 T5203 det the,failure
R327 T805 T801 dobj inclusions,manifest
R3270 T5203 T5201 pobj failure,from
R3271 T5117 T5123 advcl observed,depleted
R3272 T5204 T5205 aux to,develop
R3273 T5205 T5203 acl develop,failure
R3274 T5206 T5207 det a,trophoblast
R3275 T5118 T5119 det a,defect
R3276 T5207 T5205 dobj trophoblast,develop
R3277 T5208 T5207 amod normal,trophoblast
R3278 T5209 T5210 punct (,see
R3279 T5119 T5117 nsubjpass defect,observed
R328 T806 T801 punct ", ",manifest
R3280 T5210 T5194 parataxis see,likely
R3281 T5211 T5210 advmod below,see
R3282 T5212 T5210 punct ),see
R3283 T5213 T5166 punct .,suggest
R3285 T5495 T5496 compound Trophectoderm,Failure
R3286 T5497 T5496 prep in,Failure
R3287 T5498 T5499 amod Atrxnull,Embryos
R3288 T5499 T5497 pobj Embryos,in
R3289 T5501 T5502 amod Whole,mount
R329 T807 T801 cc but,manifest
R3290 T5502 T5504 compound mount,observation
R3291 T5503 T5502 punct -,mount
R3292 T5504 T5505 nsubj observation,revealed
R3293 T5506 T5504 prep of,observation
R3294 T5507 T5508 nummod 8.5,dpc
R3295 T5508 T5509 compound dpc,embryos
R3296 T5509 T5506 pobj embryos,of
R3297 T5510 T5511 mark that,appeared
R3298 T5511 T5505 ccomp appeared,revealed
R3299 T5512 T5511 punct ", ",appeared
R330 T808 T801 conj are,manifest
R3300 T5513 T5511 prep in,appeared
R3301 T5514 T5513 pobj contrast,in
R3302 T5515 T5514 prep to,contrast
R3303 T5516 T5517 det the,morphology
R3304 T5517 T5515 pobj morphology,to
R3305 T5518 T5519 advmod basically,normal
R3306 T5519 T5517 amod normal,morphology
R3307 T5520 T5521 mark although,delayed
R3308 T5521 T5519 parataxis delayed,normal
R3309 T5522 T5517 prep of,morphology
R331 T809 T808 advmod otherwise,are
R3310 T5523 T5524 det the,embryo
R3311 T5524 T5522 pobj embryo,of
R3312 T5525 T5524 appos itself,embryo
R3313 T5526 T5511 punct ", ",appeared
R3314 T5527 T5528 det the,tissues
R3315 T5528 T5511 nsubj tissues,appeared
R3316 T5529 T5528 amod extraembryonic,tissues
R3317 T5530 T5528 prep of,tissues
R3318 T5531 T5532 amod Atrxnull,conceptuses
R3319 T5532 T5530 pobj conceptuses,of
R332 T810 T811 advmod intellectually,normal
R3320 T5533 T5534 advmod highly,disorganised
R3321 T5534 T5511 oprd disorganised,appeared
R3322 T5535 T5505 punct .,revealed
R3323 T5537 T5538 advmod When,removed
R3324 T5538 T5541 advcl removed,appeared
R3325 T5539 T5538 nsubjpass embryos,removed
R3326 T5540 T5538 auxpass were,removed
R3327 T5542 T5538 prep from,removed
R3328 T5543 T5542 pobj deciduas,from
R3329 T5544 T5541 punct ", ",appeared
R333 T811 T808 acomp normal,are
R3330 T5545 T5546 det the,layer
R3331 T5546 T5541 nsubj layer,appeared
R3332 T5547 T5546 amod surrounding,layer
R3333 T5548 T5546 compound trophectoderm,layer
R3334 T5549 T5550 advmod dramatically,reduced
R3335 T5550 T5541 oprd reduced,appeared
R3336 T5551 T5541 prep in,appeared
R3337 T5552 T5553 amod Atrxnull,embryos
R3338 T5553 T5551 pobj embryos,in
R3339 T5554 T5541 advcl relative,appeared
R334 T812 T810 cc and,intellectually
R3340 T5555 T5554 prep to,relative
R3341 T5556 T5557 amod wild,type
R3342 T5557 T5559 compound type,littermates
R3343 T5558 T5557 punct -,type
R3344 T5559 T5555 pobj littermates,to
R3345 T5560 T5541 punct ", ",appeared
R3346 T5561 T5541 cc and,appeared
R3347 T5562 T5563 det the,cone
R3348 T5563 T5566 nsubj cone,appeared
R3349 T5564 T5563 amod underlying,cone
R335 T813 T810 conj physically,intellectually
R3350 T5565 T5563 amod ectoplacental,cone
R3351 T5566 T5541 conj appeared,appeared
R3352 T5567 T5566 oprd reduced,appeared
R3353 T5568 T5567 cc and,reduced
R3354 T5569 T5570 advmod abnormally,shaped
R3355 T5570 T5567 conj shaped,reduced
R3356 T5571 T5572 punct (,7A
R3357 T5572 T5566 parataxis 7A,appeared
R3358 T5573 T5572 compound Figure,7A
R3359 T5574 T5572 punct ),7A
R336 T814 T801 punct .,manifest
R3360 T5575 T5566 punct .,appeared
R3361 T5577 T5578 amod Vacated,deciduas
R3362 T5578 T5579 nsubjpass deciduas,bisected
R3363 T5580 T5578 acl surrounding,deciduas
R3364 T5581 T5582 nummod 8.5,dpc
R3365 T5582 T5583 nmod dpc,embryos
R3366 T5583 T5580 dobj embryos,surrounding
R3367 T5584 T5585 amod wild,type
R3368 T5585 T5583 nmod type,embryos
R3369 T5586 T5585 punct -,type
R337 T816 T817 nsubj Studies,demonstrated
R3370 T5587 T5585 cc and,type
R3371 T5588 T5585 conj Atrxnull,type
R3372 T5589 T5579 auxpass were,bisected
R3373 T5590 T5579 cc and,bisected
R3374 T5591 T5579 conj analysed,bisected
R3375 T5592 T5591 prep by,analysed
R3376 T5593 T5592 pobj WMISH,by
R3377 T5594 T5591 prep for,analysed
R3378 T5595 T5594 pobj expression,for
R3379 T5596 T5595 prep of,expression
R338 T818 T816 prep of,Studies
R3380 T5597 T5598 amod placental,lactogen
R3381 T5598 T5596 pobj lactogen,of
R3382 T5599 T5598 punct -,lactogen
R3383 T5600 T5598 nummod 1,lactogen
R3384 T5601 T5598 punct (,lactogen
R3385 T5602 T5598 appos Pl,lactogen
R3386 T5603 T5602 punct -,Pl
R3387 T5604 T5602 nummod 1,Pl
R3388 T5605 T5598 punct ),lactogen
R3389 T5606 T5598 punct ", ",lactogen
R339 T819 T820 compound X,chromosome
R3390 T5607 T5608 det a,marker
R3391 T5608 T5598 appos marker,lactogen
R3392 T5609 T5608 prep of,marker
R3393 T5610 T5611 advmod terminally,differentiated
R3394 T5611 T5612 amod differentiated,TGCs
R3395 T5612 T5609 pobj TGCs,of
R3396 T5613 T5579 punct .,bisected
R3397 T5615 T5616 det The,number
R3398 T5616 T5617 nsubj number,is
R3399 T5618 T5616 prep of,number
R340 T820 T822 compound chromosome,inactivation
R3400 T5619 T5620 npadvmod Pl,expressing
R3401 T5620 T5624 amod expressing,cells
R3402 T5621 T5619 punct -,Pl
R3403 T5622 T5619 nummod 1,Pl
R3404 T5623 T5620 punct -,expressing
R3405 T5624 T5618 pobj cells,of
R3406 T5625 T5624 acl attached,cells
R3407 T5626 T5625 prep to,attached
R3408 T5627 T5628 det the,wall
R3409 T5628 T5626 pobj wall,to
R341 T821 T820 punct -,chromosome
R3410 T5629 T5628 amod decidual,wall
R3411 T5630 T5625 prep after,attached
R3412 T5631 T5630 pobj removal,after
R3413 T5632 T5631 prep of,removal
R3414 T5633 T5634 det the,embryo
R3415 T5634 T5632 pobj embryo,of
R3416 T5635 T5636 det an,indication
R3417 T5636 T5617 attr indication,is
R3418 T5637 T5636 prep of,indication
R3419 T5638 T5639 det the,density
R342 T822 T818 pobj inactivation,of
R3420 T5639 T5637 pobj density,of
R3421 T5640 T5639 prep of,density
R3422 T5641 T5642 compound trophoblast,cells
R3423 T5642 T5640 pobj cells,of
R3424 T5643 T5642 acl surrounding,cells
R3425 T5644 T5645 det each,site
R3426 T5645 T5643 dobj site,surrounding
R3427 T5646 T5645 compound implantation,site
R3428 T5647 T5648 punct [,17
R3429 T5648 T5617 parataxis 17,is
R343 T823 T822 prep in,inactivation
R3430 T5649 T5648 punct ],17
R3431 T5650 T5617 punct .,is
R3432 T5652 T5653 nsubj We,found
R3433 T5653 T5654 ccomp found,was
R3434 T5655 T5656 mark that,depleted
R3435 T5656 T5653 ccomp depleted,found
R3436 T5657 T5658 det the,population
R3437 T5658 T5656 nsubjpass population,depleted
R3438 T5659 T5658 prep of,population
R3439 T5660 T5661 npadvmod Pl,expressing
R344 T824 T825 compound carrier,females
R3440 T5661 T5665 amod expressing,cells
R3441 T5662 T5660 punct -,Pl
R3442 T5663 T5660 nummod 1,Pl
R3443 T5664 T5661 punct -,expressing
R3444 T5665 T5659 pobj cells,of
R3445 T5666 T5656 auxpass was,depleted
R3446 T5667 T5656 prep in,depleted
R3447 T5668 T5669 det the,sites
R3448 T5669 T5667 pobj sites,in
R3449 T5670 T5669 amod decidual,sites
R345 T825 T823 pobj females,in
R3450 T5671 T5669 compound implantation,sites
R3451 T5672 T5669 acl containing,sites
R3452 T5673 T5674 amod Atrxnull,embryos
R3453 T5674 T5672 dobj embryos,containing
R3454 T5675 T5656 advcl relative,depleted
R3455 T5676 T5675 prep to,relative
R3456 T5677 T5676 pobj those,to
R3457 T5678 T5677 acl containing,those
R3458 T5679 T5680 amod wild,type
R3459 T5680 T5682 compound type,littermates
R346 T826 T817 aux have,demonstrated
R3460 T5681 T5680 punct -,type
R3461 T5682 T5678 dobj littermates,containing
R3462 T5683 T5684 punct (,7B
R3463 T5684 T5653 parataxis 7B,found
R3464 T5685 T5684 compound Figure,7B
R3465 T5686 T5684 punct ),7B
R3466 T5687 T5654 punct ;,was
R3467 T5688 T5654 nsubj this,was
R3468 T5689 T5654 advmod also,was
R3469 T5690 T5654 acomp apparent,was
R347 T827 T828 amod preferential,inactivation
R3470 T5691 T5654 prep at,was
R3471 T5692 T5693 nummod 7.5,dpc
R3472 T5693 T5691 pobj dpc,at
R3473 T5694 T5654 punct ", ",was
R3474 T5695 T5696 mark as,determined
R3475 T5696 T5654 advcl determined,was
R3476 T5697 T5696 prep by,determined
R3477 T5698 T5699 amod immunohistochemical,staining
R3478 T5699 T5697 pobj staining,by
R3479 T5700 T5699 prep of,staining
R348 T828 T817 dobj inactivation,demonstrated
R3480 T5701 T5702 compound paraffin,sections
R3481 T5702 T5700 pobj sections,of
R3482 T5703 T5702 prep of,sections
R3483 T5704 T5703 pobj embryos,of
R3484 T5705 T5704 prep in,embryos
R3485 T5706 T5705 pobj deciduas,in
R3486 T5707 T5699 prep with,staining
R3487 T5708 T5709 det an,antibody
R3488 T5709 T5707 pobj antibody,with
R3489 T5710 T5711 amod anti-Pl,1
R349 T829 T828 prep of,inactivation
R3490 T5711 T5709 nummod 1,antibody
R3491 T5712 T5711 punct -,1
R3492 T5713 T5714 punct (,7C
R3493 T5714 T5654 parataxis 7C,was
R3494 T5715 T5714 compound Figure,7C
R3495 T5716 T5714 punct ),7C
R3496 T5717 T5654 punct .,was
R3497 T5719 T5720 det A,deficiency
R3498 T5720 T5722 nsubj deficiency,is
R3499 T5721 T5720 compound TGC,deficiency
R350 T830 T831 det the,chromosome
R3500 T5723 T5722 prep in,is
R3501 T5724 T5725 det the,absence
R3502 T5725 T5723 pobj absence,in
R3503 T5726 T5725 prep of,absence
R3504 T5727 T5726 pobj Atrx,of
R3505 T5728 T5722 acomp consistent,is
R3506 T5729 T5728 prep with,consistent
R3507 T5730 T5731 det the,observation
R3508 T5731 T5729 pobj observation,with
R3509 T5732 T5733 mark that,is
R351 T831 T829 pobj chromosome,of
R3510 T5733 T5731 acl is,observation
R3511 T5734 T5733 nsubj Atrx,is
R3512 T5735 T5736 advmod highly,expressed
R3513 T5736 T5733 acomp expressed,is
R3514 T5737 T5733 prep in,is
R3515 T5738 T5739 amod giant,cells
R3516 T5739 T5737 pobj cells,in
R3517 T5740 T5739 acl surrounding,cells
R3518 T5741 T5742 amod wild,type
R3519 T5742 T5744 nmod type,embryos
R352 T832 T831 acl bearing,chromosome
R3520 T5743 T5742 punct -,type
R3521 T5744 T5740 dobj embryos,surrounding
R3522 T5745 T5746 nummod 7.5,dpc
R3523 T5746 T5744 compound dpc,embryos
R3524 T5747 T5748 punct (,5E
R3525 T5748 T5722 parataxis 5E,is
R3526 T5749 T5748 compound Figure,5E
R3527 T5750 T5748 punct ),5E
R3528 T5751 T5722 punct .,is
R3529 T5753 T5754 aux To,investigate
R353 T833 T834 det the,allele
R3530 T5754 T5755 advcl investigate,cultured
R3531 T5756 T5757 mark whether,restricted
R3532 T5757 T5754 ccomp restricted,investigate
R3533 T5758 T5759 det the,defect
R3534 T5759 T5757 nsubjpass defect,restricted
R3535 T5760 T5759 compound trophoblast,defect
R3536 T5761 T5757 auxpass was,restricted
R3537 T5762 T5757 prep to,restricted
R3538 T5763 T5764 det the,production
R3539 T5764 T5762 pobj production,to
R354 T834 T832 dobj allele,bearing
R3540 T5765 T5764 prep of,production
R3541 T5766 T5767 amod secondary,TGCs
R3542 T5767 T5765 pobj TGCs,of
R3543 T5768 T5767 punct (,TGCs
R3544 T5769 T5767 acl produced,TGCs
R3545 T5770 T5769 agent by,produced
R3546 T5771 T5772 compound diploid,precursors
R3547 T5772 T5770 pobj precursors,by
R3548 T5773 T5769 prep in,produced
R3549 T5774 T5775 det the,cone
R355 T835 T834 amod abnormal,allele
R3550 T5775 T5773 pobj cone,in
R3551 T5776 T5775 amod ectoplacental,cone
R3552 T5777 T5769 cc and,produced
R3553 T5778 T5769 conj derived,produced
R3554 T5779 T5778 advmod originally,derived
R3555 T5780 T5778 prep from,derived
R3556 T5781 T5782 det the,trophectoderm
R3557 T5782 T5780 pobj trophectoderm,from
R3558 T5783 T5782 amod polar,trophectoderm
R3559 T5784 T5782 acl overlying,trophectoderm
R356 T836 T817 prep in,demonstrated
R3560 T5785 T5786 det the,mass
R3561 T5786 T5784 dobj mass,overlying
R3562 T5787 T5786 amod inner,mass
R3563 T5788 T5786 compound cell,mass
R3564 T5789 T5786 prep of,mass
R3565 T5790 T5791 det the,blastocyst
R3566 T5791 T5789 pobj blastocyst,of
R3567 T5792 T5757 punct ),restricted
R3568 T5793 T5757 cc or,restricted
R3569 T5794 T5795 advmod also,affected
R357 T837 T838 det a,variety
R3570 T5795 T5757 conj affected,restricted
R3571 T5796 T5797 det the,production
R3572 T5797 T5795 dobj production,affected
R3573 T5798 T5797 prep of,production
R3574 T5799 T5800 amod primary,TGCs
R3575 T5800 T5798 pobj TGCs,of
R3576 T5801 T5800 punct (,TGCs
R3577 T5802 T5800 acl resulting,TGCs
R3578 T5803 T5802 prep from,resulting
R3579 T5804 T5803 pobj differentiation,from
R358 T838 T836 pobj variety,in
R3580 T5805 T5804 prep of,differentiation
R3581 T5806 T5807 det the,trophectoderm
R3582 T5807 T5805 pobj trophectoderm,of
R3583 T5808 T5807 amod mural,trophectoderm
R3584 T5809 T5807 prep of,trophectoderm
R3585 T5810 T5811 det the,blastocyst
R3586 T5811 T5809 pobj blastocyst,of
R3587 T5812 T5755 punct ),cultured
R3588 T5813 T5755 punct ", ",cultured
R3589 T5814 T5755 nsubjpass blastocysts,cultured
R359 T839 T838 prep of,variety
R3590 T5815 T5816 punct (,dpc
R3591 T5816 T5814 parataxis dpc,blastocysts
R3592 T5817 T5816 nummod 3.5,dpc
R3593 T5818 T5816 punct ),dpc
R3594 T5819 T5814 prep from,blastocysts
R3595 T5820 T5819 pobj crosses,from
R3596 T5821 T5820 prep between,crosses
R3597 T5822 T5823 compound Atrx WT,flox
R3598 T5823 T5825 compound flox,females
R3599 T5824 T5823 punct /,flox
R360 T840 T839 pobj tissues,of
R3600 T5825 T5821 pobj females,between
R3601 T5826 T5825 cc and,females
R3602 T5827 T5828 compound GATA1,Cre
R3603 T5828 T5830 npadvmod Cre,homozygous
R3604 T5829 T5828 punct -,Cre
R3605 T5830 T5831 amod homozygous,males
R3606 T5831 T5825 conj males,females
R3607 T5832 T5831 amod transgenic,males
R3608 T5833 T5825 punct (,females
R3609 T5834 T5835 compound Atrx WT,Y
R361 T841 T842 punct [,2
R3610 T5835 T5825 appos Y,females
R3611 T5836 T5835 punct /,Y
R3612 T5837 T5835 punct ;,Y
R3613 T5838 T5839 compound GATA1,Cre
R3614 T5839 T5835 appos Cre,Y
R3615 T5840 T5839 punct -,Cre
R3616 T5841 T5839 punct +,Cre
R3617 T5842 T5839 punct /,Cre
R3618 T5843 T5839 punct +,Cre
R3619 T5844 T5755 punct ),cultured
R362 T842 T817 parataxis 2,demonstrated
R3620 T5845 T5755 auxpass were,cultured
R3621 T5846 T5847 advmod in,vitro
R3622 T5847 T5755 advmod vitro,cultured
R3623 T5848 T5755 prep for,cultured
R3624 T5849 T5850 nummod 5,d
R3625 T5850 T5848 pobj d,for
R3626 T5851 T5852 aux to,monitor
R3627 T5852 T5755 advcl monitor,cultured
R3628 T5853 T5852 dobj outgrowth,monitor
R3629 T5854 T5853 prep of,outgrowth
R363 T843 T842 punct ],2
R3630 T5855 T5856 det the,trophoblast
R3631 T5856 T5854 pobj trophoblast,of
R3632 T5857 T5856 amod primary,trophoblast
R3633 T5858 T5755 punct .,cultured
R3634 T5860 T5861 prep After,scored
R3635 T5862 T5863 nummod 5,d
R3636 T5863 T5860 pobj d,After
R3637 T5864 T5861 punct ", ",scored
R3638 T5865 T5866 amod individual,cultures
R3639 T5866 T5861 nsubjpass cultures,scored
R364 T844 T817 punct ", ",demonstrated
R3640 T5867 T5866 compound blastocyst,cultures
R3641 T5868 T5861 auxpass were,scored
R3642 T5869 T5861 prep for,scored
R3643 T5870 T5871 det the,extent
R3644 T5871 T5869 pobj extent,for
R3645 T5872 T5871 prep of,extent
R3646 T5873 T5874 amod primary,outgrowth
R3647 T5874 T5872 pobj outgrowth,of
R3648 T5875 T5874 compound trophoblast,outgrowth
R3649 T5876 T5861 punct ", ",scored
R365 T845 T817 cc and,demonstrated
R3650 T5877 T5861 cc and,scored
R3651 T5878 T5879 det the,genotype
R3652 T5879 T5881 nsubjpass genotype,determined
R3653 T5880 T5879 compound Atrx,genotype
R3654 T5881 T5861 conj determined,scored
R3655 T5882 T5879 cc and,genotype
R3656 T5883 T5879 conj sex,genotype
R3657 T5884 T5883 prep of,sex
R3658 T5885 T5886 det the,blastocyst
R3659 T5886 T5884 pobj blastocyst,of
R366 T846 T847 det this,skewing
R3660 T5887 T5881 auxpass were,determined
R3661 T5888 T5881 prep by,determined
R3662 T5889 T5888 pobj PCR,by
R3663 T5890 T5861 punct .,scored
R3664 T5892 T5893 amod Most,blastocysts
R3665 T5893 T5894 nsubj blastocysts,hatched
R3666 T5895 T5894 prep from,hatched
R3667 T5896 T5897 det the,pellucida
R3668 T5897 T5895 pobj pellucida,from
R3669 T5898 T5897 compound zona,pellucida
R367 T847 T848 nsubjpass skewing,thought
R3670 T5899 T5894 prep within,hatched
R3671 T5900 T5901 nummod 24,h
R3672 T5901 T5899 pobj h,within
R3673 T5902 T5894 punct ", ",hatched
R3674 T5903 T5894 cc and,hatched
R3675 T5904 T5905 compound trophoblast,cells
R3676 T5905 T5906 nsubjpass cells,detected
R3677 T5906 T5894 conj detected,hatched
R3678 T5907 T5905 acl spreading,cells
R3679 T5908 T5907 prt out,spreading
R368 T848 T817 conj thought,demonstrated
R3680 T5909 T5907 prep from,spreading
R3681 T5910 T5911 det the,mass
R3682 T5911 T5909 pobj mass,from
R3683 T5912 T5911 amod inner,mass
R3684 T5913 T5911 compound cell,mass
R3685 T5914 T5906 aux could,detected
R3686 T5915 T5906 advmod usually,detected
R3687 T5916 T5906 auxpass be,detected
R3688 T5917 T5906 prep within,detected
R3689 T5918 T5919 nummod 48,h
R369 T849 T847 prep of,skewing
R3690 T5919 T5917 pobj h,within
R3691 T5920 T5919 prep of,h
R3692 T5921 T5920 pobj culture,of
R3693 T5922 T5906 punct .,detected
R3694 T5924 T5925 det No,difference
R3695 T5925 T5926 nsubjpass difference,observed
R3696 T5927 T5926 auxpass was,observed
R3697 T5928 T5926 prep in,observed
R3698 T5929 T5930 det the,rate
R3699 T5930 T5928 pobj rate,in
R370 T850 T851 compound X,inactivation
R3700 T5931 T5930 cc or,rate
R3701 T5932 T5930 conj extent,rate
R3702 T5933 T5930 prep of,rate
R3703 T5934 T5935 compound trophoblast,outgrowth
R3704 T5935 T5933 pobj outgrowth,of
R3705 T5936 T5926 prep over,observed
R3706 T5937 T5938 nummod 5,d
R3707 T5938 T5936 pobj d,over
R3708 T5939 T5938 prep of,d
R3709 T5940 T5939 pobj culture,of
R371 T851 T849 pobj inactivation,of
R3710 T5941 T5926 prep between,observed
R3711 T5942 T5943 amod Atrxnull,blastocysts
R3712 T5943 T5941 pobj blastocysts,between
R3713 T5944 T5945 punct (,Y
R3714 T5945 T5943 parataxis Y,blastocysts
R3715 T5946 T5945 amod Atrx null,Y
R3716 T5947 T5945 punct /,Y
R3717 T5948 T5945 punct ", ",Y
R3718 T5949 T5950 nsubj n,6
R3719 T5950 T5945 ccomp 6,Y
R372 T852 T851 punct -,inactivation
R3720 T5951 T5950 punct =,6
R3721 T5952 T5945 punct ),Y
R3722 T5953 T5943 cc and,blastocysts
R3723 T5954 T5943 conj blastocysts,blastocysts
R3724 T5955 T5954 acl bearing,blastocysts
R3725 T5956 T5957 det an,allele
R3726 T5957 T5955 dobj allele,bearing
R3727 T5958 T5957 compound Atrx WT,allele
R3728 T5959 T5960 punct (,6
R3729 T5960 T5954 parataxis 6,blastocysts
R373 T853 T848 auxpass is,thought
R3730 T5961 T5962 compound Atrx WT,WT
R3731 T5962 T5964 dep WT,6
R3732 T5963 T5962 punct /,WT
R3733 T5964 T5960 dep 6,6
R3734 T5965 T5964 punct ", ",6
R3735 T5966 T5964 nsubj n,6
R3736 T5967 T5964 punct =,6
R3737 T5968 T5960 punct ;,6
R3738 T5969 T5970 compound Atrx WT,null
R3739 T5970 T5972 dep null,6
R374 T854 T855 aux to,explain
R3740 T5971 T5970 punct /,null
R3741 T5972 T5960 dep 6,6
R3742 T5973 T5972 punct ", ",6
R3743 T5974 T5972 nsubj n,6
R3744 T5975 T5972 punct =,6
R3745 T5976 T5960 punct ;,6
R3746 T5977 T5978 compound Atrx WT,Y
R3747 T5978 T5960 dep Y,6
R3748 T5979 T5978 punct /,Y
R3749 T5980 T5960 punct ", ",6
R375 T855 T848 xcomp explain,thought
R3750 T5981 T5960 nsubj n,6
R3751 T5982 T5960 punct =,6
R3752 T5983 T5960 punct ),6
R3753 T5984 T5985 punct (,shown
R3754 T5985 T5954 parataxis shown,blastocysts
R3755 T5986 T5985 nsubj examples,shown
R3756 T5987 T5985 prep in,shown
R3757 T5988 T5989 compound Figure,7D
R3758 T5989 T5987 pobj 7D,in
R3759 T5990 T5985 punct ),shown
R376 T856 T857 det the,phenotype
R3760 T5991 T5926 punct ", ",observed
R3761 T5992 T5926 advcl suggesting,observed
R3762 T5993 T5994 mark that,involves
R3763 T5994 T5992 ccomp involves,suggesting
R3764 T5995 T5996 det the,defect
R3765 T5996 T5994 nsubj defect,involves
R3766 T5997 T5994 advmod specifically,involves
R3767 T5998 T5999 det the,compartment
R3768 T5999 T5994 dobj compartment,involves
R3769 T6000 T5999 amod secondary,compartment
R377 T857 T855 dobj phenotype,explain
R3770 T6001 T5999 amod giant,compartment
R3771 T6002 T5999 compound cell,compartment
R3772 T6003 T5926 punct .,observed
R3773 T6005 T6006 nsubj This,is
R3774 T6007 T6006 acomp consistent,is
R3775 T6008 T6007 prep with,consistent
R3776 T6009 T6010 det the,observation
R3777 T6010 T6008 pobj observation,with
R3778 T6011 T6012 mark that,implant
R3779 T6012 T6010 acl implant,observation
R378 T858 T857 amod mild,phenotype
R3780 T6013 T6014 amod Atrxnull,conceptuses
R3781 T6014 T6012 nsubj conceptuses,implant
R3782 T6015 T6012 advmod successfully,implant
R3783 T6016 T6012 cc and,implant
R3784 T6017 T6012 conj survive,implant
R3785 T6018 T6017 prep to,survive
R3786 T6019 T6018 pobj gastrulation,to
R3787 T6020 T6006 punct .,is
R3788 T6022 T6023 advcl Taken,suggest
R3789 T6024 T6022 advmod together,Taken
R379 T859 T857 acl observed,phenotype
R3790 T6025 T6023 punct ", ",suggest
R3791 T6026 T6027 det these,data
R3792 T6027 T6023 nsubj data,suggest
R3793 T6028 T6029 mark that,results
R3794 T6029 T6023 ccomp results,suggest
R3795 T6030 T6029 nsubj loss,results
R3796 T6031 T6030 prep of,loss
R3797 T6032 T6031 pobj Atrx,of
R3798 T6033 T6029 prep in,results
R3799 T6034 T6035 det a,defect
R380 T860 T859 prep in,observed
R3800 T6035 T6033 pobj defect,in
R3801 T6036 T6035 prep in,defect
R3802 T6037 T6036 pobj formation,in
R3803 T6038 T6037 prep of,formation
R3804 T6039 T6040 det the,trophoblast
R3805 T6040 T6038 pobj trophoblast,of
R3806 T6041 T6040 amod secondary,trophoblast
R3807 T6042 T6043 dep that,is
R3808 T6043 T6035 relcl is,defect
R3809 T6044 T6043 acomp apparent,is
R381 T861 T860 pobj carriers,in
R3810 T6045 T6043 prep from,is
R3811 T6046 T6047 nummod 7.5,dpc
R3812 T6047 T6045 pobj dpc,from
R3813 T6048 T6023 punct .,suggest
R3814 T6050 T6051 prep Despite,exhibit
R3815 T6052 T6050 pcomp initiating,Despite
R3816 T6053 T6054 amod normal,organisation
R3817 T6054 T6052 dobj organisation,initiating
R3818 T6055 T6052 prep in,initiating
R3819 T6056 T6057 det the,embryo
R382 T862 T848 punct .,thought
R3820 T6057 T6055 pobj embryo,in
R3821 T6058 T6057 amod proper,embryo
R3822 T6059 T6051 punct ", ",exhibit
R3823 T6060 T6061 amod Atrxnull,conceptuses
R3824 T6061 T6051 nsubj conceptuses,exhibit
R3825 T6062 T6063 det a,defect
R3826 T6063 T6051 dobj defect,exhibit
R3827 T6064 T6063 amod proliferative,defect
R3828 T6065 T6051 prep by,exhibit
R3829 T6066 T6067 nummod 7.5,dpc
R383 T864 T865 det The,syndrome
R3830 T6067 T6065 pobj dpc,by
R3831 T6068 T6051 cc and,exhibit
R3832 T6069 T6051 conj die,exhibit
R3833 T6070 T6069 prep by,die
R3834 T6071 T6072 quantmod around,9.5
R3835 T6072 T6073 nummod 9.5,dpc
R3836 T6073 T6070 pobj dpc,by
R3837 T6074 T6051 punct ", ",exhibit
R3838 T6075 T6076 advmod probably,due
R3839 T6076 T6051 prep due,exhibit
R384 T865 T869 nsubjpass syndrome,caused
R3840 T6077 T6076 pcomp to,due
R3841 T6078 T6079 det a,deficit
R3842 T6079 T6076 pobj deficit,due
R3843 T6080 T6079 amod nutritional,deficit
R3844 T6081 T6079 acl resulting,deficit
R3845 T6082 T6081 prep from,resulting
R3846 T6083 T6082 pobj failure,from
R3847 T6084 T6085 aux to,develop
R3848 T6085 T6083 acl develop,failure
R3849 T6086 T6087 det a,trophoblast
R385 T866 T867 compound ATR,X
R3850 T6087 T6085 dobj trophoblast,develop
R3851 T6088 T6087 amod normal,trophoblast
R3852 T6089 T6051 punct .,exhibit
R386 T867 T865 compound X,syndrome
R3860 T6481 T6480 prep from,Escape
R3861 T6482 T6483 amod Imprinted,Inactivation
R3862 T6483 T6481 pobj Inactivation,from
R3863 T6484 T6483 prep of,Inactivation
R3864 T6485 T6486 det the,Allele
R3865 T6486 T6484 pobj Allele,of
R3866 T6487 T6488 advmod Paternally,Inherited
R3867 T6488 T6486 amod Inherited,Allele
R3868 T6489 T6486 compound Atrx WT,Allele
R3869 T6490 T6480 prep in,Escape
R387 T868 T867 punct -,X
R3870 T6491 T6492 amod Extraembryonic,Tissues
R3871 T6492 T6490 pobj Tissues,in
R3872 T6493 T6492 prep of,Tissues
R3873 T6494 T6495 nmod Carrier,Mice
R3874 T6495 T6493 pobj Mice,of
R3875 T6496 T6495 amod Female,Mice
R3876 T6498 T6499 amod Female,mice
R3877 T6499 T6500 nsubjpass mice,detected
R3878 T6501 T6499 acl carrying,mice
R3879 T6502 T6503 det an,allele
R388 T870 T869 auxpass is,caused
R3880 T6503 T6501 dobj allele,carrying
R3881 T6504 T6503 amod Atrx null,allele
R3882 T6505 T6506 punct (,cre
R3883 T6506 T6499 parataxis cre,mice
R3884 T6507 T6508 compound Atrx WT,null
R3885 T6508 T6506 dep null,cre
R3886 T6509 T6508 punct /,null
R3887 T6510 T6506 punct ;,cre
R3888 T6511 T6506 compound GATA1,cre
R3889 T6512 T6506 punct -,cre
R389 T871 T869 agent by,caused
R3890 T6513 T6506 punct +,cre
R3891 T6514 T6506 punct /,cre
R3892 T6515 T6506 punct −,cre
R3893 T6516 T6506 punct ),cre
R3894 T6517 T6500 auxpass were,detected
R3895 T6518 T6500 prep at,detected
R3896 T6519 T6520 nummod 9.5,dpc
R3897 T6520 T6518 pobj dpc,at
R3898 T6521 T6522 punct (,Table
R3899 T6522 T6500 parataxis Table,detected
R390 T872 T871 pobj mutations,by
R3900 T6523 T6522 nummod 1,Table
R3901 T6524 T6522 punct ),Table
R3902 T6525 T6500 cc and,detected
R3903 T6526 T6500 conj recovered,detected
R3904 T6527 T6526 prep at,recovered
R3905 T6528 T6527 pobj birth,at
R3906 T6529 T6530 punct (,data
R3907 T6530 T6526 meta data,recovered
R3908 T6531 T6530 amod unpublished,data
R3909 T6532 T6530 punct ),data
R391 T873 T872 prep in,mutations
R3910 T6533 T6500 punct ", ",detected
R3911 T6534 T6535 mark although,was
R3912 T6535 T6500 advcl was,detected
R3913 T6536 T6535 prep at,was
R3914 T6537 T6538 preconj both,points
R3915 T6538 T6536 pobj points,at
R3916 T6539 T6538 compound time,points
R3917 T6540 T6541 det the,number
R3918 T6541 T6535 nsubj number,was
R3919 T6542 T6541 prep of,number
R392 T874 T875 det a,gene
R3920 T6543 T6544 compound carrier,females
R3921 T6544 T6542 pobj females,of
R3922 T6545 T6535 acomp lower,was
R3923 T6546 T6545 prep than,lower
R3924 T6547 T6546 pobj that,than
R3925 T6548 T6547 prep of,that
R3926 T6549 T6550 amod wild,type
R3927 T6550 T6552 nmod type,females
R3928 T6551 T6550 punct -,type
R3929 T6552 T6548 pobj females,of
R393 T875 T873 pobj gene,in
R3930 T6553 T6554 punct (,cre
R3931 T6554 T6550 parataxis cre,type
R3932 T6555 T6556 compound Atrx WT,WT
R3933 T6556 T6554 dep WT,cre
R3934 T6557 T6556 punct /,WT
R3935 T6558 T6554 punct ;,cre
R3936 T6559 T6554 compound GATA1,cre
R3937 T6560 T6554 punct -,cre
R3938 T6561 T6554 punct +,cre
R3939 T6562 T6554 punct /,cre
R394 T876 T875 punct (,gene
R3940 T6563 T6554 punct −,cre
R3941 T6564 T6554 punct ),cre
R3942 T6565 T6535 punct ", ",was
R3943 T6566 T6535 advcl suggesting,was
R3944 T6567 T6568 mark that,died
R3945 T6568 T6566 ccomp died,suggesting
R3946 T6569 T6570 det a,proportion
R3947 T6570 T6568 nsubj proportion,died
R3948 T6571 T6570 prep of,proportion
R3949 T6572 T6573 nmod carrier,embryos
R395 T877 T875 appos ATRX,gene
R3950 T6573 T6571 pobj embryos,of
R3951 T6574 T6573 amod female,embryos
R3952 T6575 T6576 advmod in,utero
R3953 T6576 T6568 advmod utero,died
R3954 T6577 T6500 punct .,detected
R3955 T6579 T6580 amod Surviving,mice
R3956 T6580 T6584 nsubj mice,were
R3957 T6581 T6580 amod adult,mice
R3958 T6582 T6580 nmod carrier,mice
R3959 T6583 T6580 amod female,mice
R396 T878 T875 punct ),gene
R3960 T6585 T6584 neg not,were
R3961 T6586 T6587 advmod phenotypically,normal
R3962 T6587 T6584 acomp normal,were
R3963 T6588 T6584 cc and,were
R3964 T6589 T6584 conj exhibited,were
R3965 T6590 T6591 amod mild,abnormalities
R3966 T6591 T6589 dobj abnormalities,exhibited
R3967 T6592 T6591 amod behavioural,abnormalities
R3968 T6593 T6584 punct ", ",were
R3969 T6594 T6595 mark although,reproduce
R397 T879 T880 dep that,comprises
R3970 T6595 T6584 advcl reproduce,were
R3971 T6596 T6595 nsubj some,reproduce
R3972 T6597 T6595 aux could,reproduce
R3973 T6598 T6584 punct .,were
R3974 T6600 T6601 prep For,derived
R3975 T6602 T6603 det all,embryos
R3976 T6603 T6600 pobj embryos,For
R3977 T6604 T6605 npadvmod Atrx WT,null
R3978 T6605 T6603 amod null,embryos
R3979 T6606 T6605 punct /,null
R398 T880 T875 relcl comprises,gene
R3980 T6607 T6603 nmod carrier,embryos
R3981 T6608 T6603 amod female,embryos
R3982 T6609 T6603 acl presented,embryos
R3983 T6610 T6609 prep in,presented
R3984 T6611 T6610 pobj Table,in
R3985 T6612 T6611 nummod 1,Table
R3986 T6613 T6601 punct ", ",derived
R3987 T6614 T6615 det the,allele
R3988 T6615 T6601 nsubjpass allele,derived
R3989 T6616 T6615 compound Atrx WT,allele
R399 T881 T882 nummod 36,exons
R3990 T6617 T6601 auxpass was,derived
R3991 T6618 T6601 advmod paternally,derived
R3992 T6619 T6601 punct ", ",derived
R3993 T6620 T6621 mark while,derived
R3994 T6621 T6601 advcl derived,derived
R3995 T6622 T6623 det the,allele
R3996 T6623 T6621 nsubjpass allele,derived
R3997 T6624 T6623 amod Atrx null,allele
R3998 T6625 T6621 auxpass was,derived
R3999 T6626 T6621 advmod maternally,derived
R400 T882 T880 dobj exons,comprises
R4000 T6627 T6601 punct .,derived
R4001 T6629 T6630 prep In,is
R4002 T6631 T6632 det the,mouse
R4003 T6632 T6629 pobj mouse,In
R4004 T6633 T6630 punct ", ",is
R4005 T6634 T6635 compound X,chromosome
R4006 T6635 T6636 compound chromosome,inactivation
R4007 T6636 T6630 nsubj inactivation,is
R4008 T6637 T6630 acomp subject,is
R4009 T6638 T6637 prep to,subject
R401 T883 T882 acl spanning,exons
R4010 T6639 T6640 amod parental,imprinting
R4011 T6640 T6638 pobj imprinting,to
R4012 T6641 T6640 prep in,imprinting
R4013 T6642 T6643 det the,trophectoderm
R4014 T6643 T6641 pobj trophectoderm,in
R4015 T6644 T6643 cc and,trophectoderm
R4016 T6645 T6646 amod primitive,lineages
R4017 T6646 T6643 conj lineages,trophectoderm
R4018 T6647 T6646 compound endoderm,lineages
R4019 T6648 T6649 dep that,give
R402 T884 T885 nummod 300,kb
R4020 T6649 T6643 relcl give,trophectoderm
R4021 T6650 T6649 dobj rise,give
R4022 T6651 T6649 prep to,give
R4023 T6652 T6653 det the,tissues
R4024 T6653 T6651 pobj tissues,to
R4025 T6654 T6653 amod extraembryonic,tissues
R4026 T6655 T6630 punct ", ",is
R4027 T6656 T6630 advcl resulting,is
R4028 T6657 T6656 prep in,resulting
R4029 T6658 T6657 pobj inactivation,in
R403 T885 T883 dobj kb,spanning
R4030 T6659 T6658 prep of,inactivation
R4031 T6660 T6661 det the,chromosome
R4032 T6661 T6659 pobj chromosome,of
R4033 T6662 T6663 amod paternal,X
R4034 T6663 T6661 compound X,chromosome
R4035 T6664 T6661 punct (,chromosome
R4036 T6665 T6661 appos Xp,chromosome
R4037 T6666 T6661 punct ),chromosome
R4038 T6667 T6668 punct [,18
R4039 T6668 T6630 parataxis 18,is
R404 T886 T885 prep of,kb
R4040 T6669 T6668 punct ],18
R4041 T6670 T6630 punct .,is
R4042 T6672 T6673 prep In,is
R4043 T6674 T6672 pobj contrast,In
R4044 T6675 T6673 punct ", ",is
R4045 T6676 T6673 prep in,is
R4046 T6677 T6676 pobj tissues,in
R4047 T6678 T6677 prep of,tissues
R4048 T6679 T6680 det the,embryo
R4049 T6680 T6678 pobj embryo,of
R405 T887 T888 amod genomic,DNA
R4050 T6681 T6680 amod proper,embryo
R4051 T6682 T6677 punct (,tissues
R4052 T6683 T6677 acl derived,tissues
R4053 T6684 T6683 prep from,derived
R4054 T6685 T6686 det the,mass
R4055 T6686 T6684 pobj mass,from
R4056 T6687 T6686 amod inner,mass
R4057 T6688 T6686 compound cell,mass
R4058 T6689 T6686 prep of,mass
R4059 T6690 T6691 det the,blastocyst
R406 T888 T886 pobj DNA,of
R4060 T6691 T6689 pobj blastocyst,of
R4061 T6692 T6673 punct ),is
R4062 T6693 T6694 compound X,inactivation
R4063 T6694 T6673 nsubj inactivation,is
R4064 T6695 T6694 punct -,inactivation
R4065 T6696 T6673 acomp random,is
R4066 T6697 T6698 punct [,19
R4067 T6698 T6673 parataxis 19,is
R4068 T6699 T6698 punct ],19
R4069 T6700 T6673 punct .,is
R407 T889 T883 prep at,spanning
R4070 T6702 T6703 advmod Thus,expected
R4071 T6704 T6703 punct ", ",expected
R4072 T6705 T6703 prep in,expected
R4073 T6706 T6707 det the,compartment
R4074 T6707 T6705 pobj compartment,in
R4075 T6708 T6707 amod extraembryonic,compartment
R4076 T6709 T6707 prep of,compartment
R4077 T6710 T6711 npadvmod Atrx WT,null
R4078 T6711 T6713 amod null,females
R4079 T6712 T6711 punct /,null
R408 T890 T891 compound Chromosome,Xq13.3
R4080 T6713 T6709 pobj females,of
R4081 T6714 T6713 compound carrier,females
R4082 T6715 T6703 punct ", ",expected
R4083 T6716 T6717 amod normal,inactivation
R4084 T6717 T6703 nsubjpass inactivation,expected
R4085 T6718 T6717 amod imprinted,inactivation
R4086 T6719 T6717 compound X,inactivation
R4087 T6720 T6717 punct -,inactivation
R4088 T6721 T6703 aux would,expected
R4089 T6722 T6703 auxpass be,expected
R409 T891 T889 pobj Xq13.3,at
R4090 T6723 T6724 aux to,result
R4091 T6724 T6703 xcomp result,expected
R4092 T6725 T6724 prep in,result
R4093 T6726 T6725 pobj silencing,in
R4094 T6727 T6726 prep of,silencing
R4095 T6728 T6729 det the,allele
R4096 T6729 T6727 pobj allele,of
R4097 T6730 T6731 advmod paternally,derived
R4098 T6731 T6729 amod derived,allele
R4099 T6732 T6729 compound Atrx WT,allele
R410 T892 T893 punct [,3
R4100 T6733 T6703 punct ", ",expected
R4101 T6734 T6703 advcl leaving,expected
R4102 T6735 T6736 advmod only,allele
R4103 T6736 T6734 dobj allele,leaving
R4104 T6737 T6736 det the,allele
R4105 T6738 T6736 amod Atrx null,allele
R4106 T6739 T6734 prep on,leaving
R4107 T6740 T6741 det the,X
R4108 T6741 T6739 pobj X,on
R4109 T6742 T6741 amod active,X
R411 T893 T869 parataxis 3,caused
R4110 T6743 T6741 amod maternal,X
R4111 T6744 T6741 punct (,X
R4112 T6745 T6741 appos Xm,X
R4113 T6746 T6734 punct ),leaving
R4114 T6747 T6734 cc and,leaving
R4115 T6748 T6749 advmod thereby,render
R4116 T6749 T6734 conj render,leaving
R4117 T6750 T6751 det the,tissues
R4118 T6751 T6749 dobj tissues,render
R4119 T6752 T6751 amod extraembryonic,tissues
R412 T894 T893 punct ],3
R4120 T6753 T6749 oprd null,render
R4121 T6754 T6753 prep for,null
R4122 T6755 T6756 amod full,length
R4123 T6756 T6758 compound length,protein
R4124 T6757 T6756 punct -,length
R4125 T6758 T6754 pobj protein,for
R4126 T6759 T6758 compound Atrx,protein
R4127 T6760 T6703 punct .,expected
R4128 T6762 T6763 advmod However,is
R4129 T6764 T6763 punct ", ",is
R413 T895 T869 punct .,caused
R4130 T6765 T6766 det the,absence
R4131 T6766 T6763 nsubj absence,is
R4132 T6767 T6766 prep of,absence
R4133 T6768 T6767 pobj Atrx,of
R4134 T6769 T6766 prep in,absence
R4135 T6770 T6771 det the,compartment
R4136 T6771 T6769 pobj compartment,in
R4137 T6772 T6771 amod extraembryonic,compartment
R4138 T6773 T6763 acomp lethal,is
R4139 T6774 T6763 prep in,is
R414 T897 T898 det This,gene
R4140 T6775 T6776 amod Atrx null,Y
R4141 T6776 T6778 compound Y,males
R4142 T6777 T6776 punct /,Y
R4143 T6778 T6774 pobj males,in
R4144 T6779 T6763 punct .,is
R4145 T6781 T6782 nsubj This,suggested
R4146 T6783 T6784 det the,possibility
R4147 T6784 T6782 dobj possibility,suggested
R4148 T6785 T6784 prep of,possibility
R4149 T6786 T6787 det an,escape
R415 T898 T899 nsubj gene,encodes
R4150 T6787 T6785 pobj escape,of
R4151 T6788 T6787 prep from,escape
R4152 T6789 T6790 amod imprinted,inactivation
R4153 T6790 T6788 pobj inactivation,from
R4154 T6791 T6790 prep of,inactivation
R4155 T6792 T6793 det the,allele
R4156 T6793 T6791 pobj allele,of
R4157 T6794 T6795 advmod paternally,derived
R4158 T6795 T6793 amod derived,allele
R4159 T6796 T6793 compound Atrx WT,allele
R416 T900 T901 nummod two,isoforms
R4160 T6797 T6782 prep in,suggested
R4161 T6798 T6799 det the,compartment
R4162 T6799 T6797 pobj compartment,in
R4163 T6800 T6799 amod extraembryonic,compartment
R4164 T6801 T6799 prep of,compartment
R4165 T6802 T6803 det a,proportion
R4166 T6803 T6801 pobj proportion,of
R4167 T6804 T6803 prep of,proportion
R4168 T6805 T6806 nmod carrier,embryos
R4169 T6806 T6804 pobj embryos,of
R417 T901 T899 dobj isoforms,encodes
R4170 T6807 T6806 amod female,embryos
R4171 T6808 T6782 punct .,suggested
R4172 T6810 T6811 aux To,investigate
R4173 T6811 T6812 advcl investigate,crossed
R4174 T6813 T6811 advmod further,investigate
R4175 T6814 T6812 punct ", ",crossed
R4176 T6815 T6812 nsubj we,crossed
R4177 T6816 T6817 amod homozygous,females
R4178 T6817 T6812 dobj females,crossed
R4179 T6818 T6819 compound Atrx flox,flox
R418 T902 T901 amod dominant,isoforms
R4180 T6819 T6817 compound flox,females
R4181 T6820 T6819 punct /,flox
R4182 T6821 T6817 cc and,females
R4183 T6822 T6823 amod homozygous,males
R4184 T6823 T6817 conj males,females
R4185 T6824 T6825 nmod GATA1,cre
R4186 T6825 T6823 nmod cre,males
R4187 T6826 T6825 punct -,cre
R4188 T6827 T6823 amod transgenic,males
R4189 T6828 T6829 punct (,cre
R419 T903 T901 compound protein,isoforms
R4190 T6829 T6823 parataxis cre,males
R4191 T6830 T6831 compound Atrx WT,Y
R4192 T6831 T6829 dep Y,cre
R4193 T6832 T6831 punct /,Y
R4194 T6833 T6829 punct ;,cre
R4195 T6834 T6829 compound GATA1,cre
R4196 T6835 T6829 punct -,cre
R4197 T6836 T6829 punct +,cre
R4198 T6837 T6829 punct /,cre
R4199 T6838 T6829 punct +,cre
R420 T904 T905 punct (,Figure
R4200 T6839 T6829 punct ),cre
R4201 T6840 T6812 punct ", ",crossed
R4202 T6841 T6842 dep which,expected
R4203 T6842 T6812 advcl expected,crossed
R4204 T6843 T6842 aux would,expected
R4205 T6844 T6842 auxpass be,expected
R4206 T6845 T6846 aux to,yield
R4207 T6846 T6842 xcomp yield,expected
R4208 T6847 T6848 advmod only,males
R4209 T6848 T6846 dobj males,yield
R421 T905 T899 parataxis Figure,encodes
R4210 T6849 T6848 amod Atrxnull,males
R4211 T6850 T6851 punct (,cre
R4212 T6851 T6848 parataxis cre,males
R4213 T6852 T6853 amod Atrxnull,Y
R4214 T6853 T6851 dep Y,cre
R4215 T6854 T6853 punct /,Y
R4216 T6855 T6851 punct ;,cre
R4217 T6856 T6851 compound GATA1,cre
R4218 T6857 T6851 punct -,cre
R4219 T6858 T6851 punct +,cre
R422 T906 T905 nummod 1,Figure
R4220 T6859 T6851 punct /,cre
R4221 T6860 T6851 punct −,cre
R4222 T6861 T6851 punct ),cre
R4223 T6862 T6848 cc and,males
R4224 T6863 T6864 compound carrier,females
R4225 T6864 T6848 conj females,males
R4226 T6865 T6866 punct (,cre
R4227 T6866 T6864 parataxis cre,females
R4228 T6867 T6868 compound Atrx WT,null
R4229 T6868 T6866 dep null,cre
R423 T907 T905 punct ),Figure
R4230 T6869 T6868 punct /,null
R4231 T6870 T6866 punct ;,cre
R4232 T6871 T6866 compound GATA1,cre
R4233 T6872 T6866 punct -,cre
R4234 T6873 T6866 punct +,cre
R4235 T6874 T6866 punct /,cre
R4236 T6875 T6866 punct −,cre
R4237 T6876 T6866 punct ),cre
R4238 T6877 T6812 punct .,crossed
R4239 T6879 T6880 prep In,inherited
R424 T908 T899 punct .,encodes
R4240 T6881 T6882 det these,females
R4241 T6882 T6879 pobj females,In
R4242 T6883 T6882 compound carrier,females
R4243 T6884 T6880 punct ", ",inherited
R4244 T6885 T6886 det the,allele
R4245 T6886 T6880 nsubjpass allele,inherited
R4246 T6887 T6886 compound Atrx WT,allele
R4247 T6888 T6880 auxpass is,inherited
R4248 T6889 T6880 advmod paternally,inherited
R4249 T6890 T6880 punct .,inherited
R425 T910 T911 advmod As,as
R4250 T6892 T6893 nsubjpass Embryos,dissected
R4251 T6894 T6893 auxpass were,dissected
R4252 T6895 T6893 prep in,dissected
R4253 T6896 T6897 poss their,deciduas
R4254 T6897 T6895 pobj deciduas,in
R4255 T6898 T6893 prep at,dissected
R4256 T6899 T6900 nummod 7.5,dpc
R4257 T6900 T6898 pobj dpc,at
R4258 T6901 T6893 punct ", ",dissected
R4259 T6902 T6893 cc and,dissected
R426 T911 T913 prep as,demonstrated
R4260 T6903 T6904 compound paraffin,sections
R4261 T6904 T6905 nsubjpass sections,stained
R4262 T6905 T6893 conj stained,dissected
R4263 T6906 T6905 auxpass were,stained
R4264 T6907 T6905 prep with,stained
R4265 T6908 T6909 amod anti-ATRX,antibody
R4266 T6909 T6907 pobj antibody,with
R4267 T6910 T6905 punct ", ",stained
R4268 T6911 T6912 cc along,sections
R4269 T6912 T6905 dep sections,stained
R427 T912 T911 advmod well,as
R4270 T6913 T6911 dep with,along
R4271 T6914 T6912 prep from,sections
R4272 T6915 T6916 det a,embryo
R4273 T6916 T6914 pobj embryo,from
R4274 T6917 T6918 amod wild,type
R4275 T6918 T6916 nmod type,embryo
R4276 T6919 T6918 punct -,type
R4277 T6920 T6921 nummod 7.5,dpc
R4278 T6921 T6916 compound dpc,embryo
R4279 T6922 T6912 prep for,sections
R428 T914 T915 det the,protein
R4280 T6923 T6922 pobj comparison,for
R4281 T6924 T6925 punct (,Figure
R4282 T6925 T6905 parataxis Figure,stained
R4283 T6926 T6925 nummod 8,Figure
R4284 T6927 T6925 punct ),Figure
R4285 T6928 T6905 punct .,stained
R4286 T6930 T6931 mark As,described
R4287 T6931 T6932 advcl described,detected
R4288 T6933 T6931 advmod above,described
R4289 T6934 T6932 punct ", ",detected
R429 T915 T911 pobj protein,as
R4290 T6935 T6936 compound Atrx,expression
R4291 T6936 T6932 nsubjpass expression,detected
R4292 T6937 T6932 auxpass was,detected
R4293 T6938 T6932 prep in,detected
R4294 T6939 T6940 det every,cell
R4295 T6940 T6938 pobj cell,in
R4296 T6941 T6940 prep in,cell
R4297 T6942 T6943 det the,region
R4298 T6943 T6941 pobj region,in
R4299 T6944 T6943 nmod epiblast,region
R430 T916 T917 amod full,length
R4300 T6945 T6944 punct (,epiblast
R4301 T6946 T6944 appos embryo,epiblast
R4302 T6947 T6946 amod proper,embryo
R4303 T6948 T6943 punct ),region
R4304 T6949 T6943 prep of,region
R4305 T6950 T6951 amod wild,type
R4306 T6951 T6953 nmod type,embryos
R4307 T6952 T6951 punct -,type
R4308 T6953 T6949 pobj embryos,of
R4309 T6954 T6955 nummod 7.5,dpc
R431 T917 T915 compound length,protein
R4310 T6955 T6953 compound dpc,embryos
R4311 T6956 T6957 punct (,8B
R4312 T6957 T6932 parataxis 8B,detected
R4313 T6958 T6957 compound Figure,8B
R4314 T6959 T6957 punct ),8B
R4315 T6960 T6932 punct .,detected
R4316 T6962 T6963 prep In,composed
R4317 T6964 T6962 pobj contrast,In
R4318 T6965 T6963 punct ", ",composed
R4319 T6966 T6967 det the,region
R432 T918 T917 punct -,length
R4320 T6967 T6963 nsubjpass region,composed
R4321 T6968 T6967 compound epiblast,region
R4322 T6969 T6967 prep of,region
R4323 T6970 T6971 nmod carrier,embryos
R4324 T6971 T6969 pobj embryos,of
R4325 T6972 T6971 amod female,embryos
R4326 T6973 T6963 auxpass was,composed
R4327 T6974 T6963 prep of,composed
R4328 T6975 T6976 det a,mosaic
R4329 T6976 T6974 pobj mosaic,of
R433 T919 T915 compound ATRX,protein
R4330 T6977 T6976 prep of,mosaic
R4331 T6978 T6979 amod small,clusters
R4332 T6979 T6977 pobj clusters,of
R4333 T6980 T6979 prep of,clusters
R4334 T6981 T6982 npadvmod Atrx,positive
R4335 T6982 T6984 amod positive,cells
R4336 T6983 T6982 punct -,positive
R4337 T6984 T6980 pobj cells,of
R4338 T6985 T6984 punct (,cells
R4339 T6986 T6987 prep in,inactivated
R434 T920 T915 prep of,protein
R4340 T6987 T6984 relcl inactivated,cells
R4341 T6988 T6986 pobj which,in
R4342 T6989 T6990 det the,allele
R4343 T6990 T6987 nsubjpass allele,inactivated
R4344 T6991 T6990 amod Atrx null,allele
R4345 T6992 T6990 prep on,allele
R4346 T6993 T6992 pobj Xm,on
R4347 T6994 T6987 aux had,inactivated
R4348 T6995 T6987 auxpass been,inactivated
R4349 T6996 T6984 punct ),cells
R435 T921 T922 punct ~,280
R4350 T6997 T6984 cc and,cells
R4351 T6998 T6999 npadvmod Atrx,negative
R4352 T6999 T7001 amod negative,cells
R4353 T7000 T6999 punct -,negative
R4354 T7001 T6984 conj cells,cells
R4355 T7002 T7001 punct (,cells
R4356 T7003 T7004 prep in,inactivated
R4357 T7004 T7001 relcl inactivated,cells
R4358 T7005 T7003 pobj which,in
R4359 T7006 T7007 det the,allele
R436 T922 T923 nummod 280,kDa
R4360 T7007 T7004 nsubjpass allele,inactivated
R4361 T7008 T7007 compound Atrx WT,allele
R4362 T7009 T7007 prep on,allele
R4363 T7010 T7009 pobj Xp,on
R4364 T7011 T7004 aux had,inactivated
R4365 T7012 T7004 auxpass been,inactivated
R4366 T7013 T6963 punct ),composed
R4367 T7014 T6963 punct ", ",composed
R4368 T7015 T6963 advcl indicating,composed
R4369 T7016 T7017 mark that,was
R437 T923 T920 pobj kDa,of
R4370 T7017 T7015 ccomp was,indicating
R4371 T7018 T7019 det the,gene
R4372 T7019 T7017 nsubj gene,was
R4373 T7020 T7019 compound Atrx,gene
R4374 T7021 T7017 acomp subject,was
R4375 T7022 T7021 prep to,subject
R4376 T7023 T7024 amod normal,inactivation
R4377 T7024 T7022 pobj inactivation,to
R4378 T7025 T7024 amod random,inactivation
R4379 T7026 T7024 compound X,inactivation
R438 T924 T915 punct ", ",protein
R4380 T7027 T7024 punct -,inactivation
R4381 T7028 T7017 prep in,was
R4382 T7029 T7030 det the,epiblast
R4383 T7030 T7028 pobj epiblast,in
R4384 T7031 T6963 punct .,composed
R4385 T7033 T7034 advmod Remarkably,detected
R4386 T7035 T7034 punct ", ",detected
R4387 T7036 T7037 amod clear,expression
R4388 T7037 T7034 nsubjpass expression,detected
R4389 T7038 T7037 compound Atrx,expression
R439 T925 T926 dep which,encoded
R4390 T7039 T7034 aux could,detected
R4391 T7040 T7034 advmod also,detected
R4392 T7041 T7034 auxpass be,detected
R4393 T7042 T7034 prep in,detected
R4394 T7043 T7044 det the,tissues
R4395 T7044 T7042 pobj tissues,in
R4396 T7045 T7044 amod extraembryonic,tissues
R4397 T7046 T7044 prep of,tissues
R4398 T7047 T7048 compound carrier,females
R4399 T7048 T7046 pobj females,of
R440 T926 T915 relcl encoded,protein
R4400 T7049 T7034 punct ", ",detected
R4401 T7050 T7051 mark as,shown
R4402 T7051 T7034 advcl shown,detected
R4403 T7052 T7051 prep in,shown
R4404 T7053 T7054 det the,ectoderm
R4405 T7054 T7052 pobj ectoderm,in
R4406 T7055 T7056 amod extraembryonic,derived
R4407 T7056 T7054 amod derived,ectoderm
R4408 T7057 T7056 punct -,derived
R4409 T7058 T7054 amod chorionic,ectoderm
R441 T927 T926 auxpass is,encoded
R4410 T7059 T7060 punct (,8C
R4411 T7060 T7034 parataxis 8C,detected
R4412 T7061 T7060 compound Figure,8C
R4413 T7062 T7060 punct ),8C
R4414 T7063 T7034 punct .,detected
R4415 T7065 T7066 compound Atrx,expression
R4416 T7066 T7067 nsubjpass expression,detected
R4417 T7068 T7067 aux could,detected
R4418 T7069 T7067 auxpass be,detected
R4419 T7070 T7067 prep in,detected
R442 T928 T926 agent by,encoded
R4420 T7071 T7072 advmod almost,all
R4421 T7072 T7073 nummod all,cells
R4422 T7073 T7070 pobj cells,in
R4423 T7074 T7073 prep of,cells
R4424 T7075 T7076 det the,ectoderm
R4425 T7076 T7074 pobj ectoderm,of
R4426 T7077 T7076 amod chorionic,ectoderm
R4427 T7078 T7067 punct .,detected
R4428 T7080 T7081 compound Atrx,expression
R4429 T7081 T7082 nsubjpass expression,detected
R443 T929 T930 det a,transcript
R4430 T7083 T7082 auxpass was,detected
R4431 T7084 T7082 advmod also,detected
R4432 T7085 T7082 advmod clearly,detected
R4433 T7086 T7082 prep in,detected
R4434 T7087 T7088 amod other,structures
R4435 T7088 T7086 pobj structures,in
R4436 T7089 T7088 amod extraembryonic,structures
R4437 T7090 T7088 punct ", ",structures
R4438 T7091 T7088 prep including,structures
R4439 T7092 T7091 pobj TGCs,including
R444 T930 T928 pobj transcript,by
R4440 T7093 T7094 punct (,data
R4441 T7094 T7082 meta data,detected
R4442 T7095 T7094 amod unpublished,data
R4443 T7096 T7094 punct ),data
R4444 T7097 T7082 punct .,detected
R4445 T7099 T7100 nsubjpass Escape,observed
R4446 T7101 T7099 prep from,Escape
R4447 T7102 T7101 pobj silencing,from
R4448 T7103 T7102 prep of,silencing
R4449 T7104 T7105 det an,allele
R445 T931 T930 prep of,transcript
R4450 T7105 T7103 pobj allele,of
R4451 T7106 T7107 npadvmod Xp,inherited
R4452 T7107 T7105 amod inherited,allele
R4453 T7108 T7107 punct -,inherited
R4454 T7109 T7105 compound Atrx WT,allele
R4455 T7110 T7100 auxpass was,observed
R4456 T7111 T7100 advmod also,observed
R4457 T7112 T7100 prep in,observed
R4458 T7113 T7114 det the,ectoderm
R4459 T7114 T7112 pobj ectoderm,in
R446 T932 T933 punct ~,10
R4460 T7115 T7114 amod chorionic,ectoderm
R4461 T7116 T7114 prep of,ectoderm
R4462 T7117 T7118 compound carrier,females
R4463 T7118 T7116 pobj females,of
R4464 T7119 T7100 prep at,observed
R4465 T7120 T7121 nummod 8.5,dpc
R4466 T7121 T7119 pobj dpc,at
R4467 T7122 T7123 punct (,S4
R4468 T7123 T7100 parataxis S4,observed
R4469 T7124 T7123 compound Figure,S4
R447 T933 T934 nummod 10,kb
R4470 T7125 T7123 punct ),S4
R4471 T7126 T7100 punct .,observed
R4472 T7128 T7129 advmod Thus,escaped
R4473 T7130 T7129 punct ", ",escaped
R4474 T7131 T7132 mark although,occurs
R4475 T7132 T7129 advcl occurs,escaped
R4476 T7133 T7134 amod random,inactivation
R4477 T7134 T7132 nsubj inactivation,occurs
R4478 T7135 T7134 compound X,inactivation
R4479 T7136 T7134 punct -,inactivation
R448 T934 T931 pobj kb,of
R4480 T7137 T7132 advmod normally,occurs
R4481 T7138 T7132 prep within,occurs
R4482 T7139 T7140 det the,epiblast
R4483 T7140 T7138 pobj epiblast,within
R4484 T7141 T7129 punct ", ",escaped
R4485 T7142 T7143 det the,allele
R4486 T7143 T7129 nsubj allele,escaped
R4487 T7144 T7143 compound Atrx WT,allele
R4488 T7145 T7143 punct (,allele
R4489 T7146 T7143 acl inherited,allele
R449 T935 T913 punct ", ",demonstrated
R4490 T7147 T7146 prep on,inherited
R4491 T7148 T7149 det the,chromosome
R4492 T7149 T7147 pobj chromosome,on
R4493 T7150 T7149 compound Xp,chromosome
R4494 T7151 T7129 punct ),escaped
R4495 T7152 T7153 det the,inactivation
R4496 T7153 T7129 dobj inactivation,escaped
R4497 T7154 T7153 amod normal,inactivation
R4498 T7155 T7153 amod imprinted,inactivation
R4499 T7156 T7153 compound X,inactivation
R450 T936 T913 nsubj we,demonstrated
R4500 T7157 T7153 punct -,inactivation
R4501 T7158 T7153 prep in,inactivation
R4502 T7159 T7160 det the,compartment
R4503 T7160 T7158 pobj compartment,in
R4504 T7161 T7160 amod extraembryonic,compartment
R4505 T7162 T7160 prep of,compartment
R4506 T7163 T7164 det some,females
R4507 T7164 T7162 pobj females,of
R4508 T7165 T7164 compound carrier,females
R4509 T7166 T7129 punct .,escaped
R451 T937 T913 advmod recently,demonstrated
R4510 T7194 T7195 nsubj We,investigated
R4511 T7196 T7197 det the,role
R4512 T7197 T7195 dobj role,investigated
R4513 T7198 T7197 prep of,role
R4514 T7199 T7200 det the,protein
R4515 T7200 T7198 pobj protein,of
R4516 T7201 T7200 compound Atrx,protein
R4517 T7202 T7197 prep in,role
R4518 T7203 T7204 compound mouse,development
R4519 T7204 T7202 pobj development,in
R452 T938 T939 mark that,produced
R4520 T7205 T7195 punct .,investigated
R4521 T7207 T7208 prep By,ablated
R4522 T7209 T7207 pcomp using,By
R4523 T7210 T7211 det a,approach
R4524 T7211 T7209 dobj approach,using
R4525 T7212 T7211 amod conditional,approach
R4526 T7213 T7211 compound knockout,approach
R4527 T7214 T7208 punct ", ",ablated
R4528 T7215 T7208 nsubj we,ablated
R4529 T7216 T7217 det the,protein
R453 T939 T913 ccomp produced,demonstrated
R4530 T7217 T7208 dobj protein,ablated
R4531 T7218 T7219 amod full,length
R4532 T7219 T7217 compound length,protein
R4533 T7220 T7219 punct -,length
R4534 T7221 T7217 compound Atrx,protein
R4535 T7222 T7223 advmod first,in
R4536 T7223 T7208 prep in,ablated
R4537 T7224 T7225 compound ES,cells
R4538 T7225 T7223 pobj cells,in
R4539 T7226 T7225 cc and,cells
R454 T940 T941 det a,isoform
R4540 T7227 T7228 amod embryoid,bodies
R4541 T7228 T7225 conj bodies,cells
R4542 T7229 T7223 punct ", ",in
R4543 T7230 T7223 cc and,in
R4544 T7231 T7232 advmod then,in
R4545 T7232 T7223 conj in,in
R4546 T7233 T7234 amod developing,embryos
R4547 T7234 T7232 pobj embryos,in
R4548 T7235 T7234 compound mouse,embryos
R4549 T7236 T7208 punct .,ablated
R455 T941 T939 nsubjpass isoform,produced
R4550 T7416 T7415 prep in,Atrx
R4551 T7417 T7418 compound ES,Cells
R4552 T7418 T7416 pobj Cells,in
R4553 T7420 T7421 amod Atrxnull,ES
R4554 T7421 T7422 compound ES,cells
R4555 T7422 T7423 nsubjpass cells,recovered
R4556 T7424 T7423 aux could,recovered
R4557 T7425 T7423 neg not,recovered
R4558 T7426 T7423 auxpass be,recovered
R4559 T7427 T7423 agent by,recovered
R456 T942 T941 amod truncated,isoform
R4560 T7428 T7429 amod direct,targeting
R4561 T7429 T7427 pobj targeting,by
R4562 T7430 T7423 cc and,recovered
R4563 T7431 T7432 auxpass were,generated
R4564 T7432 T7423 conj generated,recovered
R4565 T7433 T7432 advmod eventually,generated
R4566 T7434 T7432 prep by,generated
R4567 T7435 T7434 pcomp adopting,by
R4568 T7436 T7437 det a,approach
R4569 T7437 T7435 dobj approach,adopting
R457 T943 T941 acl called,isoform
R4570 T7438 T7439 amod conditional,targeting
R4571 T7439 T7437 compound targeting,approach
R4572 T7440 T7423 punct .,recovered
R4573 T7442 T7443 nsubj This,is
R4574 T7444 T7443 acomp consistent,is
R4575 T7445 T7444 prep with,consistent
R4576 T7446 T7447 poss our,observation
R4577 T7447 T7445 pobj observation,with
R4578 T7448 T7449 mark that,is
R4579 T7449 T7447 advcl is,observation
R458 T944 T943 oprd ATRXt,called
R4580 T7450 T7449 nsubj Atrx,is
R4581 T7451 T7452 advmod highly,expressed
R4582 T7452 T7449 acomp expressed,is
R4583 T7453 T7449 prep in,is
R4584 T7454 T7455 compound ES,cells
R4585 T7455 T7453 pobj cells,in
R4586 T7456 T7449 punct ", ",is
R4587 T7457 T7449 cc and,is
R4588 T7458 T7459 mark that,imparts
R4589 T7459 T7449 conj imparts,is
R459 T945 T946 punct (,kDa
R4590 T7460 T7461 det the,absence
R4591 T7461 T7459 nsubj absence,imparts
R4592 T7462 T7461 prep of,absence
R4593 T7463 T7464 amod full,length
R4594 T7464 T7466 compound length,Atrx
R4595 T7465 T7464 punct -,length
R4596 T7466 T7462 pobj Atrx,of
R4597 T7467 T7468 det a,disadvantage
R4598 T7468 T7459 dobj disadvantage,imparts
R4599 T7469 T7468 compound growth,disadvantage
R460 T946 T941 parataxis kDa,isoform
R4600 T7470 T7459 advcl relative,imparts
R4601 T7471 T7470 prep to,relative
R4602 T7472 T7471 pobj cells,to
R4603 T7473 T7472 acl bearing,cells
R4604 T7474 T7475 det a,allele
R4605 T7475 T7473 dobj allele,bearing
R4606 T7476 T7475 amod functional,allele
R4607 T7477 T7475 compound Atrx,allele
R4608 T7478 T7443 punct .,is
R4609 T7480 T7481 prep At,is
R461 T947 T948 punct ~,200
R4610 T7482 T7480 amod present,At
R4611 T7483 T7481 punct ", ",is
R4612 T7484 T7485 det the,cause
R4613 T7485 T7481 nsubj cause,is
R4614 T7486 T7485 prep of,cause
R4615 T7487 T7488 det the,delay
R4616 T7488 T7486 pobj delay,of
R4617 T7489 T7488 amod proliferative,delay
R4618 T7490 T7488 prep in,delay
R4619 T7491 T7492 amod Atrxnull,cells
R462 T948 T946 nummod 200,kDa
R4620 T7492 T7490 pobj cells,in
R4621 T7493 T7492 compound ES,cells
R4622 T7494 T7481 neg not,is
R4623 T7495 T7481 acomp known,is
R4624 T7496 T7481 punct .,is
R4625 T7498 T7499 advmod Interestingly,demonstrated
R4626 T7500 T7499 punct ", ",demonstrated
R4627 T7501 T7499 nsubj we,demonstrated
R4628 T7502 T7503 mark that,regulated
R4629 T7503 T7499 ccomp regulated,demonstrated
R463 T949 T946 punct ),kDa
R4630 T7504 T7503 nsubjpass apoptosis,regulated
R4631 T7505 T7503 auxpass is,regulated
R4632 T7506 T7503 neg not,regulated
R4633 T7507 T7503 advmod significantly,regulated
R4634 T7508 T7503 advmod up,regulated
R4635 T7509 T7503 punct -,regulated
R4636 T7510 T7503 prep in,regulated
R4637 T7511 T7512 compound ES,cells
R4638 T7512 T7510 pobj cells,in
R4639 T7513 T7512 acl lacking,cells
R464 T950 T939 auxpass is,produced
R4640 T7514 T7513 dobj Atrx,lacking
R4641 T7515 T7503 cc and,regulated
R4642 T7516 T7503 conj is,regulated
R4643 T7517 T7518 advmod only,mildly
R4644 T7518 T7519 advmod mildly,elevated
R4645 T7519 T7516 acomp elevated,is
R4646 T7520 T7516 prep in,is
R4647 T7521 T7522 amod Atrxnull,embryos
R4648 T7522 T7520 pobj embryos,in
R4649 T7523 T7524 nummod 7.5,dpc
R465 T951 T939 prep from,produced
R4650 T7524 T7522 compound dpc,embryos
R4651 T7525 T7522 compound mouse,embryos
R4652 T7526 T7499 punct .,demonstrated
R4653 T7528 T7529 prep In,shown
R4654 T7530 T7528 pobj contrast,In
R4655 T7531 T7529 punct ", ",shown
R4656 T7532 T7529 nsubjpass it,shown
R4657 T7533 T7529 auxpass was,shown
R4658 T7534 T7529 advmod recently,shown
R4659 T7535 T7536 mark that,increased
R466 T952 T953 det a,transcript
R4660 T7536 T7529 ccomp increased,shown
R4661 T7537 T7538 det the,loss
R4662 T7538 T7536 nsubj loss,increased
R4663 T7539 T7538 prep of,loss
R4664 T7540 T7539 pobj Atrx,of
R4665 T7541 T7536 advmod markedly,increased
R4666 T7542 T7543 det the,population
R4667 T7543 T7536 dobj population,increased
R4668 T7544 T7543 amod apoptotic,population
R4669 T7545 T7536 prep in,increased
R467 T953 T951 pobj transcript,from
R4670 T7546 T7547 det the,cells
R4671 T7547 T7545 pobj cells,in
R4672 T7548 T7547 amod differentiating,cells
R4673 T7549 T7547 prep of,cells
R4674 T7550 T7551 det the,cortex
R4675 T7551 T7549 pobj cortex,of
R4676 T7552 T7551 amod embryonic,cortex
R4677 T7553 T7551 cc and,cortex
R4678 T7554 T7555 amod postnatal,hippocampus
R4679 T7555 T7551 conj hippocampus,cortex
R468 T954 T953 prep of,transcript
R4680 T7556 T7536 punct ", ",increased
R4681 T7557 T7558 advmod when,ablated
R4682 T7558 T7536 advcl ablated,increased
R4683 T7559 T7560 compound Atrx,expression
R4684 T7560 T7558 nsubjpass expression,ablated
R4685 T7561 T7558 auxpass was,ablated
R4686 T7562 T7558 prep in,ablated
R4687 T7563 T7564 det the,forebrain
R4688 T7564 T7562 pobj forebrain,in
R4689 T7565 T7564 amod developing,forebrain
R469 T955 T956 quantmod around,7
R4690 T7566 T7564 compound mouse,forebrain
R4691 T7567 T7536 advcl using,increased
R4692 T7568 T7569 det the,allele
R4693 T7569 T7567 dobj allele,using
R4694 T7570 T7569 compound Atrx flox,allele
R4695 T7571 T7569 acl described,allele
R4696 T7572 T7571 advmod here,described
R4697 T7573 T7574 punct [,20
R4698 T7574 T7529 parataxis 20,shown
R4699 T7575 T7574 punct ],20
R470 T956 T957 nummod 7,kb
R4700 T7576 T7529 punct .,shown
R4701 T7578 T7579 det The,protein
R4702 T7579 T7582 nsubjpass protein,shown
R4703 T7580 T7579 amod human,protein
R4704 T7581 T7579 compound ATRX,protein
R4705 T7583 T7582 aux has,shown
R4706 T7584 T7582 auxpass been,shown
R4707 T7585 T7586 aux to,associate
R4708 T7586 T7582 xcomp associate,shown
R4709 T7587 T7586 prep in,associate
R471 T957 T954 pobj kb,of
R4710 T7588 T7589 det a,complex
R4711 T7589 T7587 pobj complex,in
R4712 T7590 T7586 prep with,associate
R4713 T7591 T7590 pobj Daxx,with
R4714 T7592 T7593 punct [,8
R4715 T7593 T7591 parataxis 8,Daxx
R4716 T7594 T7593 punct ],8
R4717 T7595 T7591 punct ", ",Daxx
R4718 T7596 T7597 det a,protein
R4719 T7597 T7591 appos protein,Daxx
R472 T958 T953 punct ", ",transcript
R4720 T7598 T7599 dep that,implicated
R4721 T7599 T7597 relcl implicated,protein
R4722 T7600 T7599 aux has,implicated
R4723 T7601 T7599 auxpass been,implicated
R4724 T7602 T7599 prep in,implicated
R4725 T7603 T7604 amod multiple,pathways
R4726 T7604 T7602 pobj pathways,in
R4727 T7605 T7599 prep for,implicated
R4728 T7606 T7607 det the,regulation
R4729 T7607 T7605 pobj regulation,for
R473 T959 T960 dep which,arises
R4730 T7608 T7607 prep of,regulation
R4731 T7609 T7608 pobj apoptosis,of
R4732 T7610 T7611 punct [,21
R4733 T7611 T7582 parataxis 21,shown
R4734 T7612 T7611 punct ],21
R4735 T7613 T7582 punct .,shown
R4736 T7615 T7616 nsubj It,is
R4737 T7617 T7616 acomp possible,is
R4738 T7618 T7619 mark that,triggered
R4739 T7619 T7616 ccomp triggered,is
R474 T960 T953 relcl arises,transcript
R4740 T7620 T7619 nsubj disruption,triggered
R4741 T7621 T7620 prep of,disruption
R4742 T7622 T7623 det the,complex
R4743 T7623 T7621 pobj complex,of
R4744 T7624 T7623 compound mouse,complex
R4745 T7625 T7626 compound Atrx,Daxx
R4746 T7626 T7623 compound Daxx,complex
R4747 T7627 T7626 punct -,Daxx
R4748 T7628 T7620 punct (,disruption
R4749 T7629 T7620 prep by,disruption
R475 T961 T962 advmod when,fails
R4750 T7630 T7629 pobj ablation,by
R4751 T7631 T7630 prep of,ablation
R4752 T7632 T7633 det the,protein
R4753 T7633 T7631 pobj protein,of
R4754 T7634 T7633 compound Atrx,protein
R4755 T7635 T7619 punct ),triggered
R4756 T7636 T7619 aux could,triggered
R4757 T7637 T7619 aux have,triggered
R4758 T7638 T7639 det a,response
R4759 T7639 T7619 dobj response,triggered
R476 T962 T960 advcl fails,arises
R4760 T7640 T7639 amod universal,response
R4761 T7641 T7639 amod proapoptotic,response
R4762 T7642 T7616 punct .,is
R4763 T7644 T7645 advmod However,demonstrate
R4764 T7646 T7645 punct ", ",demonstrate
R4765 T7647 T7648 poss our,observations
R4766 T7648 T7645 nsubj observations,demonstrate
R4767 T7649 T7648 prep in,observations
R4768 T7650 T7651 compound ES,cells
R4769 T7651 T7649 pobj cells,in
R477 T963 T962 nsubj intron,fails
R4770 T7652 T7653 mark that,is
R4771 T7653 T7645 ccomp is,demonstrate
R4772 T7654 T7655 det the,induction
R4773 T7655 T7653 nsubj induction,is
R4774 T7656 T7655 prep of,induction
R4775 T7657 T7656 pobj apoptosis,of
R4776 T7658 T7653 neg not,is
R4777 T7659 T7660 det an,response
R4778 T7660 T7653 attr response,is
R4779 T7661 T7660 amod automatic,response
R478 T964 T963 nummod 11,intron
R4780 T7662 T7660 acl triggered,response
R4781 T7663 T7662 agent by,triggered
R4782 T7664 T7665 det the,removal
R4783 T7665 T7663 pobj removal,by
R4784 T7666 T7665 prep of,removal
R4785 T7667 T7666 pobj Atrx,of
R4786 T7668 T7662 prep in,triggered
R4787 T7669 T7670 det all,types
R4788 T7670 T7668 pobj types,in
R4789 T7671 T7670 compound cell,types
R479 T965 T966 aux to,spliced
R4790 T7672 T7645 punct ", ",demonstrate
R4791 T7673 T7645 cc and,demonstrate
R4792 T7674 T7645 conj suggest,demonstrate
R4793 T7675 T7676 mark that,reflect
R4794 T7676 T7674 ccomp reflect,suggest
R4795 T7677 T7678 det the,apoptosis
R4796 T7678 T7676 nsubj apoptosis,reflect
R4797 T7679 T7678 amod inappropriate,apoptosis
R4798 T7680 T7678 acl observed,apoptosis
R4799 T7681 T7680 prep in,observed
R480 T966 T962 xcomp spliced,fails
R4800 T7682 T7683 det the,forebrain
R4801 T7683 T7681 pobj forebrain,in
R4802 T7684 T7685 compound Atrx,mutant
R4803 T7685 T7683 compound mutant,forebrain
R4804 T7686 T7685 punct -,mutant
R4805 T7687 T7676 aux may,reflect
R4806 T7688 T7689 det a,requirement
R4807 T7689 T7676 dobj requirement,reflect
R4808 T7690 T7689 prep for,requirement
R4809 T7691 T7690 pobj Atrx,for
R481 T967 T966 auxpass be,spliced
R4810 T7692 T7689 prep during,requirement
R4811 T7693 T7694 amod terminal,differentiation
R4812 T7694 T7692 pobj differentiation,during
R4813 T7695 T7645 punct .,demonstrate
R482 T968 T966 prep from,spliced
R4824 T8323 T8324 det An,Role
R4825 T8325 T8324 amod Unexpected,Role
R4826 T8326 T8324 prep for,Role
R4827 T8327 T8326 pobj Atrx,for
R4828 T8328 T8324 prep in,Role
R4829 T8329 T8328 pobj Development,in
R483 T969 T970 det the,transcript
R4830 T8330 T8329 prep of,Development
R4831 T8331 T8332 det the,Trophoblast
R4832 T8332 T8330 pobj Trophoblast,of
R4833 T8333 T8332 compound Mouse,Trophoblast
R4834 T8335 T8336 nsubj We,show
R4835 T8337 T8338 mark that,are
R4836 T8338 T8336 ccomp are,show
R4837 T8339 T8340 amod Atrxnull,mice
R4838 T8340 T8338 nsubj mice,are
R4839 T8341 T8340 amod male,mice
R484 T970 T968 pobj transcript,from
R4840 T8342 T8338 neg not,are
R4841 T8343 T8338 acomp viable,are
R4842 T8344 T8338 cc and,are
R4843 T8345 T8346 nsubj embryos,die
R4844 T8346 T8338 conj die,are
R4845 T8347 T8346 prep by,die
R4846 T8348 T8349 quantmod around,9.5
R4847 T8349 T8350 nummod 9.5,dpc
R4848 T8350 T8347 pobj dpc,by
R4849 T8351 T8336 punct .,show
R485 T971 T970 amod primary,transcript
R4850 T8353 T8354 prep Before,exhibit
R4851 T8355 T8353 pobj death,Before
R4852 T8356 T8354 punct ", ",exhibit
R4853 T8357 T8358 amod Atrxnull,embryos
R4854 T8358 T8354 nsubj embryos,exhibit
R4855 T8359 T8360 det a,index
R4856 T8360 T8354 dobj index,exhibit
R4857 T8361 T8362 advmod markedly,reduced
R4858 T8362 T8360 amod reduced,index
R4859 T8363 T8360 amod mitotic,index
R486 T972 T962 cc and,fails
R4860 T8364 T8354 punct ", ",exhibit
R4861 T8365 T8354 advcl suggesting,exhibit
R4862 T8366 T8367 det a,defect
R4863 T8367 T8365 dobj defect,suggesting
R4864 T8368 T8367 amod proliferative,defect
R4865 T8369 T8354 punct .,exhibit
R4866 T8371 T8372 mark Although,appears
R4867 T8372 T8379 advcl appears,display
R4868 T8373 T8374 det the,compartment
R4869 T8374 T8372 nsubj compartment,appears
R487 T973 T974 det an,signal
R4870 T8375 T8374 amod embryonic,compartment
R4871 T8376 T8374 prep of,compartment
R4872 T8377 T8378 det the,conceptus
R4873 T8378 T8376 pobj conceptus,of
R4874 T8380 T8381 advmod initially,normal
R4875 T8381 T8372 oprd normal,appears
R4876 T8382 T8379 punct ", ",display
R4877 T8383 T8379 prep by,display
R4878 T8384 T8385 nummod 7.5,dpc
R4879 T8385 T8383 pobj dpc,by
R488 T974 T981 nsubjpass signal,used
R4880 T8386 T8387 amod Atrxnull,embryos
R4881 T8387 T8379 nsubj embryos,display
R4882 T8388 T8379 dobj abnormalities,display
R4883 T8389 T8388 prep in,abnormalities
R4884 T8390 T8389 pobj development,in
R4885 T8391 T8390 prep of,development
R4886 T8392 T8393 det the,trophoblast
R4887 T8393 T8391 pobj trophoblast,of
R4888 T8394 T8388 punct ", ",abnormalities
R4889 T8395 T8388 prep including,abnormalities
R489 T975 T974 amod alternative,signal
R4890 T8396 T8397 det a,depletion
R4891 T8397 T8395 pobj depletion,including
R4892 T8398 T8397 prep in,depletion
R4893 T8399 T8400 det the,population
R4894 T8400 T8398 pobj population,in
R4895 T8401 T8400 prep of,population
R4896 T8402 T8401 pobj TGCs,of
R4897 T8403 T8402 acl surrounding,TGCs
R4898 T8404 T8405 det the,conceptus
R4899 T8405 T8403 dobj conceptus,surrounding
R490 T976 T974 amod intronic,signal
R4900 T8406 T8397 cc and,depletion
R4901 T8407 T8408 det a,reduction
R4902 T8408 T8397 conj reduction,depletion
R4903 T8409 T8408 prep in,reduction
R4904 T8410 T8411 det the,size
R4905 T8411 T8409 pobj size,in
R4906 T8412 T8411 prep of,size
R4907 T8413 T8414 det the,cone
R4908 T8414 T8412 pobj cone,of
R4909 T8415 T8414 amod ectoplacental,cone
R491 T977 T974 nmod poly,signal
R4910 T8416 T8414 punct ", ",cone
R4911 T8417 T8418 dep which,contains
R4912 T8418 T8414 relcl contains,cone
R4913 T8419 T8420 det the,precursors
R4914 T8420 T8418 dobj precursors,contains
R4915 T8421 T8420 nmod diploid,precursors
R4916 T8422 T8420 amod giant,precursors
R4917 T8423 T8420 compound cell,precursors
R4918 T8424 T8425 punct [,22
R4919 T8425 T8379 parataxis 22,display
R492 T978 T979 punct (,A
R4920 T8426 T8425 punct ],22
R4921 T8427 T8379 punct .,display
R4922 T8429 T8430 nsubj TGCs,are
R4923 T8431 T8432 advmod highly,differentiated
R4924 T8432 T8433 amod differentiated,cells
R4925 T8433 T8430 attr cells,are
R4926 T8434 T8433 punct ", ",cells
R4927 T8435 T8433 amod postmitotic,cells
R4928 T8436 T8437 dep that,form
R4929 T8437 T8433 relcl form,cells
R493 T979 T974 parataxis A,signal
R4930 T8438 T8437 advmod ultimately,form
R4931 T8439 T8440 det an,layer
R4932 T8440 T8437 dobj layer,form
R4933 T8441 T8440 amod epithelial,layer
R4934 T8442 T8437 prep at,form
R4935 T8443 T8444 det the,periphery
R4936 T8444 T8442 pobj periphery,at
R4937 T8445 T8444 prep of,periphery
R4938 T8446 T8447 det the,placenta
R4939 T8447 T8445 pobj placenta,of
R494 T980 T979 punct ),A
R4940 T8448 T8437 punct ", ",form
R4941 T8449 T8450 dep which,interfaces
R4942 T8450 T8437 ccomp interfaces,form
R4943 T8451 T8450 prep with,interfaces
R4944 T8452 T8453 det the,cells
R4945 T8453 T8451 pobj cells,with
R4946 T8454 T8453 amod maternal,cells
R4947 T8455 T8453 prep of,cells
R4948 T8456 T8457 det the,decidua
R4949 T8457 T8455 pobj decidua,of
R495 T981 T962 conj used,fails
R4950 T8458 T8459 punct [,23
R4951 T8459 T8430 parataxis 23,are
R4952 T8460 T8459 punct ],23
R4953 T8461 T8430 punct .,are
R4954 T8463 T8464 det These,cells
R4955 T8464 T8467 nsubj cells,are
R4956 T8465 T8466 advmod highly,invasive
R4957 T8466 T8464 amod invasive,cells
R4958 T8468 T8467 acomp important,are
R4959 T8469 T8468 prep for,important
R496 T982 T981 auxpass is,used
R4960 T8470 T8469 pcomp mediating,for
R4961 T8471 T8472 amod initial,invasion
R4962 T8472 T8470 dobj invasion,mediating
R4963 T8473 T8472 prep of,invasion
R4964 T8474 T8475 det the,tissue
R4965 T8475 T8473 pobj tissue,of
R4966 T8476 T8475 amod uterine,tissue
R4967 T8477 T8467 punct ", ",are
R4968 T8478 T8467 cc but,are
R4969 T8479 T8480 auxpass are,involved
R497 T983 T984 punct [,4
R4970 T8480 T8467 conj involved,are
R4971 T8481 T8480 advmod also,involved
R4972 T8482 T8480 prep in,involved
R4973 T8483 T8482 pcomp remodelling,in
R4974 T8484 T8485 det the,decidua
R4975 T8485 T8483 dobj decidua,remodelling
R4976 T8486 T8485 amod maternal,decidua
R4977 T8487 T8483 prep after,remodelling
R4978 T8488 T8487 pobj implantation,after
R4979 T8489 T8482 cc and,in
R498 T984 T913 parataxis 4,demonstrated
R4980 T8490 T8482 conj in,in
R4981 T8491 T8490 pcomp secreting,in
R4982 T8492 T8491 dobj hormones,secreting
R4983 T8493 T8494 dep that,regulate
R4984 T8494 T8492 relcl regulate,hormones
R4985 T8495 T8496 amod fetal,growth
R4986 T8496 T8494 dobj growth,regulate
R4987 T8497 T8495 cc and,fetal
R4988 T8498 T8495 conj maternal,fetal
R4989 T8499 T8500 punct [,24
R499 T985 T984 punct ],4
R4990 T8500 T8480 parataxis 24,involved
R4991 T8501 T8500 punct ],24
R4992 T8502 T8467 punct .,are
R4993 T8504 T8505 mark Since,appear
R4994 T8505 T8508 advcl appear,is
R4995 T8506 T8507 amod Atrxnull,embryos
R4996 T8507 T8505 nsubj embryos,appear
R4997 T8509 T8510 aux to,implant
R4998 T8510 T8505 xcomp implant,appear
R4999 T8511 T8510 advmod normally,implant
R500 T986 T913 punct .,demonstrated
R5000 T8512 T8508 punct ", ",is
R5001 T8513 T8508 nsubj lethality,is
R5002 T8514 T8508 acomp likely,is
R5003 T8515 T8516 aux to,arise
R5004 T8516 T8514 xcomp arise,likely
R5005 T8517 T8516 prep due,arise
R5006 T8518 T8517 pcomp to,due
R5007 T8519 T8520 det a,failure
R5008 T8520 T8517 pobj failure,due
R5009 T8521 T8520 prep of,failure
R501 T988 T989 det The,homolog
R5010 T8522 T8523 compound TGC,function
R5011 T8523 T8521 pobj function,of
R5012 T8524 T8516 advmod later,arise
R5013 T8525 T8516 prep during,arise
R5014 T8526 T8525 pobj development,during
R5015 T8527 T8508 punct .,is
R5016 T8529 T8530 amod Embryonic,lethality
R5017 T8530 T8531 nsubj lethality,was
R5018 T8532 T8530 prep in,lethality
R5019 T8533 T8532 pobj mice,in
R502 T989 T991 nsubjpass homolog,situated
R5020 T8534 T8530 prep in,lethality
R5021 T8535 T8536 det the,absence
R5022 T8536 T8534 pobj absence,in
R5023 T8537 T8536 prep of,absence
R5024 T8538 T8537 pobj Atrx,of
R5025 T8539 T8540 det a,finding
R5026 T8540 T8531 attr finding,was
R5027 T8541 T8540 amod surprising,finding
R5028 T8542 T8531 punct ", ",was
R5029 T8543 T8544 mark as,been
R503 T990 T989 compound mouse,homolog
R5030 T8544 T8531 advcl been,was
R5031 T8545 T8544 expl there,been
R5032 T8546 T8544 aux had,been
R5033 T8547 T8548 det no,suggestion
R5034 T8548 T8544 attr suggestion,been
R5035 T8549 T8548 prep of,suggestion
R5036 T8550 T8551 amod foetal,loss
R5037 T8551 T8549 pobj loss,of
R5038 T8552 T8544 prep in,been
R5039 T8553 T8554 det the,syndrome
R504 T992 T989 prep of,homolog
R5040 T8554 T8552 pobj syndrome,in
R5041 T8555 T8554 amod human,syndrome
R5042 T8556 T8557 compound ATR,X
R5043 T8557 T8554 compound X,syndrome
R5044 T8558 T8557 punct -,X
R5045 T8559 T8531 punct .,was
R5046 T8561 T8562 nsubj It,is
R5047 T8563 T8562 acomp possible,is
R5048 T8564 T8565 mark that,is
R5049 T8565 T8562 advcl is,is
R505 T993 T994 det the,gene
R5050 T8566 T8567 det the,role
R5051 T8567 T8565 nsubj role,is
R5052 T8568 T8567 prep of,role
R5053 T8569 T8568 pobj Atrx,of
R5054 T8570 T8567 prep in,role
R5055 T8571 T8572 det the,trophoblast
R5056 T8572 T8570 pobj trophoblast,in
R5057 T8573 T8565 acomp specific,is
R5058 T8574 T8573 prep to,specific
R5059 T8575 T8574 pobj mice,to
R506 T994 T992 pobj gene,of
R5060 T8576 T8565 cc and,is
R5061 T8577 T8578 mark that,has
R5062 T8578 T8565 conj has,is
R5063 T8579 T8578 nsubj ATRX,has
R5064 T8580 T8581 det no,role
R5065 T8581 T8578 dobj role,has
R5066 T8582 T8578 cc or,has
R5067 T8583 T8578 conj is,has
R5068 T8584 T8583 acomp redundant,is
R5069 T8585 T8578 prep in,has
R507 T995 T994 compound ATRX,gene
R5070 T8586 T8587 det the,trophoblast
R5071 T8587 T8585 pobj trophoblast,in
R5072 T8588 T8587 amod human,trophoblast
R5073 T8589 T8562 punct .,is
R5074 T8591 T8592 advmod Indeed,is
R5075 T8593 T8592 punct ", ",is
R5076 T8594 T8595 det the,weight
R5077 T8595 T8592 nsubj weight,is
R5078 T8596 T8595 compound birth,weight
R5079 T8597 T8595 prep of,weight
R508 T996 T989 punct ", ",homolog
R5080 T8598 T8597 pobj babies,of
R5081 T8599 T8598 prep with,babies
R5082 T8600 T8601 compound ATR,X
R5083 T8601 T8603 compound X,syndrome
R5084 T8602 T8601 punct -,X
R5085 T8603 T8599 pobj syndrome,with
R5086 T8604 T8592 advmod usually,is
R5087 T8605 T8592 acomp normal,is
R5088 T8606 T8592 punct .,is
R5089 T8608 T8609 det An,explanation
R509 T997 T989 appos Atrx,homolog
R5090 T8609 T8611 nsubj explanation,is
R5091 T8610 T8609 amod alternative,explanation
R5092 T8612 T8609 prep for,explanation
R5093 T8613 T8614 det the,phenotype
R5094 T8614 T8612 pobj phenotype,for
R5095 T8615 T8616 advmod unexpectedly,severe
R5096 T8616 T8614 amod severe,phenotype
R5097 T8617 T8618 nsubj we,observed
R5098 T8618 T8614 advcl observed,phenotype
R5099 T8619 T8618 prep in,observed
R510 T998 T991 punct ", ",situated
R5100 T8620 T8619 pobj mice,in
R5101 T8621 T8622 mark that,ablates
R5102 T8622 T8611 ccomp ablates,is
R5103 T8623 T8624 det the,deletion
R5104 T8624 T8622 nsubj deletion,ablates
R5105 T8625 T8624 compound Atrx Δ18Δneo,deletion
R5106 T8626 T8624 acl generated,deletion
R5107 T8627 T8626 prep by,generated
R5108 T8628 T8629 compound Cre,recombination
R5109 T8629 T8627 pobj recombination,by
R511 T999 T991 auxpass is,situated
R5110 T8630 T8622 advmod completely,ablates
R5111 T8631 T8632 amod full,length
R5112 T8632 T8634 compound length,protein
R5113 T8633 T8632 punct -,length
R5114 T8634 T8622 dobj protein,ablates
R5115 T8635 T8634 compound Atrx,protein
R5116 T8636 T8637 punct (,2E
R5117 T8637 T8611 parataxis 2E,is
R5118 T8638 T8637 compound Figure,2E
R5119 T8639 T8637 punct ),2E
R512 T1000 T991 advmod also,situated
R5120 T8640 T8611 punct .,is
R5121 T8642 T8643 prep In,appear
R5122 T8644 T8642 pobj contrast,In
R5123 T8645 T8643 punct ", ",appear
R5124 T8646 T8647 det all,mutations
R5125 T8647 T8643 nsubj mutations,appear
R5126 T8648 T8649 npadvmod disease,causing
R5127 T8649 T8647 amod causing,mutations
R5128 T8650 T8649 punct -,causing
R5129 T8651 T8647 acl characterised,mutations
R513 T1001 T991 prep on,situated
R5130 T8652 T8651 prep in,characterised
R5131 T8653 T8654 amod human,pedigrees
R5132 T8654 T8652 pobj pedigrees,in
R5133 T8655 T8656 compound ATR,X
R5134 T8656 T8654 compound X,pedigrees
R5135 T8657 T8656 punct -,X
R5136 T8658 T8659 aux to,give
R5137 T8659 T8643 xcomp give,appear
R5138 T8660 T8659 dobj rise,give
R5139 T8661 T8659 prep to,give
R514 T1002 T1003 det the,chromosome
R5140 T8662 T8663 amod hypomorphic,alleles
R5141 T8663 T8661 pobj alleles,to
R5142 T8664 T8643 punct .,appear
R5143 T8666 T8667 det Some,protein
R5144 T8667 T8672 nsubjpass protein,detected
R5145 T8668 T8669 amod full,length
R5146 T8669 T8667 compound length,protein
R5147 T8670 T8669 punct -,length
R5148 T8671 T8667 compound ATRX,protein
R5149 T8673 T8672 auxpass is,detected
R515 T1003 T1001 pobj chromosome,on
R5150 T8674 T8672 prep in,detected
R5151 T8675 T8674 pobj cases,in
R5152 T8676 T8675 acl predicted,cases
R5153 T8677 T8678 aux to,have
R5154 T8678 T8676 xcomp have,predicted
R5155 T8679 T8680 compound truncating,mutations
R5156 T8680 T8678 dobj mutations,have
R5157 T8681 T8682 punct (,data
R5158 T8682 T8672 parataxis data,detected
R5159 T8683 T8682 dep RJG,data
R516 T1004 T1003 compound X,chromosome
R5160 T8684 T8682 punct ", ",data
R5161 T8685 T8682 amod unpublished,data
R5162 T8686 T8682 punct ),data
R5163 T8687 T8672 punct ", ",detected
R5164 T8688 T8672 cc and,detected
R5165 T8689 T8690 amod residual,activity
R5166 T8690 T8692 nsubjpass activity,detected
R5167 T8691 T8690 compound ATPase,activity
R5168 T8692 T8672 conj detected,detected
R5169 T8693 T8690 prep in,activity
R517 T1005 T991 punct ", ",situated
R5170 T8694 T8695 compound ATRX,immunoprecipitates
R5171 T8695 T8693 pobj immunoprecipitates,in
R5172 T8696 T8692 aux can,detected
R5173 T8697 T8692 auxpass be,detected
R5174 T8698 T8692 prep in,detected
R5175 T8699 T8700 compound Epstein,Barr
R5176 T8700 T8702 compound Barr,virus
R5177 T8701 T8700 punct -,Barr
R5178 T8702 T8703 npadvmod virus,transformed
R5179 T8703 T8705 amod transformed,lymphocytes
R518 T1006 T991 cc and,situated
R5180 T8704 T8703 punct -,transformed
R5181 T8705 T8698 pobj lymphocytes,in
R5182 T8706 T8705 prep of,lymphocytes
R5183 T8707 T8708 det all,patients
R5184 T8708 T8706 pobj patients,of
R5185 T8709 T8708 amod human,patients
R5186 T8710 T8708 acl analysed,patients
R5187 T8711 T8710 prep to,analysed
R5188 T8712 T8711 pobj date,to
R5189 T8713 T8714 punct (,A.
R519 T1007 T1008 advmod also,gives
R5190 T8714 T8692 meta A.,detected
R5191 T8715 T8714 nmod Argentaro,A.
R5192 T8716 T8714 cc and,A.
R5193 T8717 T8714 conj M.,A.
R5194 T8718 T8717 nmod Mitson,M.
R5195 T8719 T8717 punct ", ",M.
R5196 T8720 T8721 amod unpublished,data
R5197 T8721 T8717 conj data,M.
R5198 T8722 T8721 punct ),data
R5199 T8723 T8692 punct .,detected
R520 T1008 T991 conj gives,situated
R5200 T8725 T8726 det The,failure
R5201 T8726 T8727 nsubj failure,suggests
R5202 T8728 T8729 aux to,observe
R5203 T8729 T8726 acl observe,failure
R5204 T8730 T8731 det a,allele
R5205 T8731 T8729 dobj allele,observe
R5206 T8732 T8733 advmod truly,null
R5207 T8733 T8731 amod null,allele
R5208 T8734 T8731 compound ATRX,allele
R5209 T8735 T8729 prep among,observe
R521 T1009 T1008 dobj rise,gives
R5210 T8736 T8737 amod human,patients
R5211 T8737 T8735 pobj patients,among
R5212 T8738 T8727 advmod strongly,suggests
R5213 T8739 T8740 mark that,is
R5214 T8740 T8727 ccomp is,suggests
R5215 T8741 T8740 punct ", ",is
R5216 T8742 T8740 prep as,is
R5217 T8743 T8742 prep in,as
R5218 T8744 T8745 det the,mouse
R5219 T8745 T8743 pobj mouse,in
R522 T1010 T1008 prep to,gives
R5220 T8746 T8740 punct ", ",is
R5221 T8747 T8748 det the,absence
R5222 T8748 T8740 nsubj absence,is
R5223 T8749 T8748 amod complete,absence
R5224 T8750 T8748 prep of,absence
R5225 T8751 T8752 compound ATRX,protein
R5226 T8752 T8750 pobj protein,of
R5227 T8753 T8740 acomp incompatible,is
R5228 T8754 T8753 prep with,incompatible
R5229 T8755 T8756 amod human,survival
R523 T1011 T1012 amod full,length
R5230 T8756 T8754 pobj survival,with
R5231 T8757 T8756 amod fetal,survival
R5232 T8758 T8727 punct .,suggests
R5233 T8760 T8761 mark While,revealed
R5234 T8761 T8765 advcl revealed,is
R5235 T8762 T8763 det this,study
R5236 T8763 T8761 nsubj study,revealed
R5237 T8764 T8761 aux has,revealed
R5238 T8766 T8767 det an,role
R5239 T8767 T8761 dobj role,revealed
R524 T1012 T1014 nmod length,isoforms
R5240 T8768 T8767 amod unexpected,role
R5241 T8769 T8767 prep for,role
R5242 T8770 T8769 pobj Atrx,for
R5243 T8771 T8767 prep in,role
R5244 T8772 T8773 det the,trophectoderm
R5245 T8773 T8771 pobj trophectoderm,in
R5246 T8774 T8773 amod murine,trophectoderm
R5247 T8775 T8761 punct ", ",revealed
R5248 T8776 T8761 prep as,revealed
R5249 T8777 T8778 det a,result
R525 T1013 T1012 punct -,length
R5250 T8778 T8776 pobj result,as
R5251 T8779 T8778 prep of,result
R5252 T8780 T8781 det the,lethality
R5253 T8781 T8779 pobj lethality,of
R5254 T8782 T8781 amod early,lethality
R5255 T8783 T8781 acl observed,lethality
R5256 T8784 T8783 prep in,observed
R5257 T8785 T8786 amod Atrxnull,embryos
R5258 T8786 T8784 pobj embryos,in
R5259 T8787 T8765 nsubj it,is
R526 T1014 T1010 pobj isoforms,to
R5260 T8788 T8765 neg not,is
R5261 T8789 T8765 acomp possible,is
R5262 T8790 T8791 aux to,rule
R5263 T8791 T8765 xcomp rule,is
R5264 T8792 T8791 prt out,rule
R5265 T8793 T8794 amod other,roles
R5266 T8794 T8791 dobj roles,rule
R5267 T8795 T8794 prep for,roles
R5268 T8796 T8795 pobj Atrx,for
R5269 T8797 T8794 prep at,roles
R527 T1015 T1016 punct (,kDa
R5270 T8798 T8799 amod later,stages
R5271 T8799 T8797 pobj stages,at
R5272 T8800 T8799 amod developmental,stages
R5273 T8801 T8794 prep in,roles
R5274 T8802 T8801 pobj tissues,in
R5275 T8803 T8802 prep of,tissues
R5276 T8804 T8805 det the,embryo
R5277 T8805 T8803 pobj embryo,of
R5278 T8806 T8805 amod proper,embryo
R5279 T8807 T8765 punct .,is
R528 T1016 T1012 parataxis kDa,length
R5280 T8809 T8810 advmod Indeed,show
R5281 T8811 T8810 punct ", ",show
R5282 T8812 T8810 nsubj we,show
R5283 T8813 T8814 mark that,is
R5284 T8814 T8810 ccomp is,show
R5285 T8815 T8814 nsubj Atrx,is
R5286 T8816 T8817 advmod highly,expressed
R5287 T8817 T8814 acomp expressed,is
R5288 T8818 T8814 prep throughout,is
R5289 T8819 T8820 det the,embryo
R529 T1017 T1016 dep Atrx,kDa
R5290 T8820 T8818 pobj embryo,throughout
R5291 T8821 T8820 amod entire,embryo
R5292 T8822 T8820 amod developing,embryo
R5293 T8823 T8814 prep at,is
R5294 T8824 T8825 nummod 7.5,dpc
R5295 T8825 T8823 pobj dpc,at
R5296 T8826 T8827 punct (,5B
R5297 T8827 T8814 parataxis 5B,is
R5298 T8828 T8827 compound Figure,5B
R5299 T8829 T8827 punct ),5B
R530 T1018 T1016 punct ", ",kDa
R5300 T8830 T8810 punct ", ",show
R5301 T8831 T8810 cc and,show
R5302 T8832 T8833 nsubj it,is
R5303 T8833 T8810 conj is,show
R5304 T8834 T8833 acomp likely,is
R5305 T8835 T8836 mark that,turn
R5306 T8836 T8833 ccomp turn,is
R5307 T8837 T8838 compound Atrx,function
R5308 T8838 T8836 nsubj function,turn
R5309 T8839 T8836 aux will,turn
R531 T1019 T1020 punct ~,280
R5310 T8840 T8836 prt out,turn
R5311 T8841 T8842 aux to,be
R5312 T8842 T8836 xcomp be,turn
R5313 T8843 T8842 acomp important,be
R5314 T8844 T8843 prep for,important
R5315 T8845 T8846 amod other,tissues
R5316 T8846 T8844 pobj tissues,for
R5317 T8847 T8846 amod differentiating,tissues
R5318 T8848 T8810 punct .,show
R5319 T8850 T8851 compound Tetraploid,aggregation
R532 T1020 T1016 nummod 280,kDa
R5320 T8851 T8852 compound aggregation,experiments
R5321 T8852 T8853 nsubj experiments,shed
R5322 T8854 T8852 punct (,experiments
R5323 T8855 T8856 prep in,rescued
R5324 T8856 T8852 relcl rescued,experiments
R5325 T8857 T8855 pobj which,in
R5326 T8858 T8859 compound mutant,embryos
R5327 T8859 T8856 nsubjpass embryos,rescued
R5328 T8860 T8856 auxpass are,rescued
R5329 T8861 T8856 prep with,rescued
R533 T1021 T1016 punct ),kDa
R5330 T8862 T8863 amod wild,type
R5331 T8863 T8865 nmod type,tissues
R5332 T8864 T8863 punct -,type
R5333 T8865 T8861 pobj tissues,with
R5334 T8866 T8865 amod extraembryonic,tissues
R5335 T8867 T8853 punct ),shed
R5336 T8868 T8853 aux might,shed
R5337 T8869 T8870 amod more,light
R5338 T8870 T8853 dobj light,shed
R5339 T8871 T8853 prep on,shed
R534 T1022 T1012 cc and,length
R5340 T8872 T8873 det the,role
R5341 T8873 T8871 pobj role,on
R5342 T8874 T8873 prep of,role
R5343 T8875 T8874 pobj Atrx,of
R5344 T8876 T8873 prep during,role
R5345 T8877 T8878 amod later,development
R5346 T8878 T8876 pobj development,during
R5347 T8879 T8878 compound mouse,development
R5348 T8880 T8853 punct ", ",shed
R5349 T8881 T8853 cc but,shed
R535 T1023 T1012 conj truncated,length
R5350 T8882 T8883 det these,issues
R5351 T8883 T8884 nsubjpass issues,dissected
R5352 T8884 T8853 conj dissected,shed
R5353 T8885 T8884 aux can,dissected
R5354 T8886 T8884 auxpass be,dissected
R5355 T8887 T8888 advmod more,subtly
R5356 T8888 T8884 advmod subtly,dissected
R5357 T8889 T8884 prep by,dissected
R5358 T8890 T8889 pcomp combining,by
R5359 T8891 T8892 det the,allele
R536 T1024 T1025 punct (,kDa
R5360 T8892 T8890 dobj allele,combining
R5361 T8893 T8892 amod conditional,allele
R5362 T8894 T8892 compound Atrx flox,allele
R5363 T8895 T8896 dep that,generated
R5364 T8896 T8892 relcl generated,allele
R5365 T8897 T8896 nsubj we,generated
R5366 T8898 T8896 aux have,generated
R5367 T8899 T8890 prep with,combining
R5368 T8900 T8901 amod different,transgenes
R5369 T8901 T8899 pobj transgenes,with
R537 T1025 T1023 parataxis kDa,truncated
R5370 T8902 T8903 npadvmod tissue,specific
R5371 T8903 T8901 amod specific,transgenes
R5372 T8904 T8903 punct -,specific
R5373 T8905 T8901 compound Cre,transgenes
R5374 T8906 T8884 punct .,dissected
R5375 T8908 T8909 mark As,mentioned
R5376 T8909 T8910 advcl mentioned,revealed
R5377 T8911 T8909 advmod above,mentioned
R5378 T8912 T8910 punct ", ",revealed
R5379 T8913 T8914 det this,approach
R538 T1026 T1025 dep Atrxt,kDa
R5380 T8914 T8910 nsubj approach,revealed
R5381 T8915 T8910 aux has,revealed
R5382 T8916 T8910 advmod already,revealed
R5383 T8917 T8918 det a,role
R5384 T8918 T8910 dobj role,revealed
R5385 T8919 T8918 amod critical,role
R5386 T8920 T8918 prep for,role
R5387 T8921 T8920 pobj Atrx,for
R5388 T8922 T8918 prep during,role
R5389 T8923 T8924 amod neuronal,differentiation
R539 T1027 T1025 punct ", ",kDa
R5390 T8924 T8922 pobj differentiation,during
R5391 T8925 T8918 prep in,role
R5392 T8926 T8927 amod adult,mice
R5393 T8927 T8925 pobj mice,in
R5394 T8928 T8929 punct [,20
R5395 T8929 T8910 parataxis 20,revealed
R5396 T8930 T8929 punct ],20
R5397 T8931 T8910 punct .,revealed
R5398 T8933 T8934 amod Further,evidence
R5399 T8934 T8935 nsubjpass evidence,provided
R540 T1028 T1029 punct ~,200
R5400 T8936 T8937 mark that,required
R5401 T8937 T8934 acl required,evidence
R5402 T8938 T8937 nsubjpass Atrx,required
R5403 T8939 T8937 auxpass is,required
R5404 T8940 T8937 advmod also,required
R5405 T8941 T8937 prep at,required
R5406 T8942 T8943 amod later,stages
R5407 T8943 T8941 pobj stages,at
R5408 T8944 T8943 prep of,stages
R5409 T8945 T8946 compound mouse,development
R541 T1029 T1025 nummod 200,kDa
R5410 T8946 T8944 pobj development,of
R5411 T8947 T8935 auxpass is,provided
R5412 T8948 T8935 agent by,provided
R5413 T8949 T8950 det the,skewing
R5414 T8950 T8948 pobj skewing,by
R5415 T8951 T8950 amod observed,skewing
R5416 T8952 T8950 amod dramatic,skewing
R5417 T8953 T8950 prep against,skewing
R5418 T8954 T8955 nmod Atrx,cells
R5419 T8955 T8953 pobj cells,against
R542 T1030 T1025 punct ),kDa
R5420 T8956 T8954 punct -,Atrx
R5421 T8957 T8954 amod negative,Atrx
R5422 T8958 T8950 prep in,skewing
R5423 T8959 T8960 det some,tissues
R5424 T8960 T8958 pobj tissues,in
R5425 T8961 T8960 amod somatic,tissues
R5426 T8962 T8960 prep of,tissues
R5427 T8963 T8964 nmod carrier,mice
R5428 T8964 T8962 pobj mice,of
R5429 T8965 T8964 amod female,mice
R543 T1031 T1032 punct [,5
R5430 T8966 T8964 punct ", ",mice
R5431 T8967 T8968 poss whose,tissues
R5432 T8968 T8969 dep tissues,comprise
R5433 T8969 T8964 relcl comprise,mice
R5434 T8970 T8969 advmod initially,comprise
R5435 T8971 T8972 det a,mosaic
R5436 T8972 T8969 dobj mosaic,comprise
R5437 T8973 T8972 prep of,mosaic
R5438 T8974 T8975 npadvmod Atrx,positive
R5439 T8975 T8977 amod positive,cells
R544 T1032 T1008 parataxis 5,gives
R5440 T8976 T8975 punct -,positive
R5441 T8977 T8973 pobj cells,of
R5442 T8978 T8975 cc and,positive
R5443 T8979 T8980 npadvmod Atrx,negative
R5444 T8980 T8975 conj negative,positive
R5445 T8981 T8980 punct -,negative
R5446 T8982 T8969 prep as,comprise
R5447 T8983 T8984 det a,result
R5448 T8984 T8982 pobj result,as
R5449 T8985 T8984 prep of,result
R545 T1033 T1032 nummod 4,5
R5450 T8986 T8987 amod random,inactivation
R5451 T8987 T8985 pobj inactivation,of
R5452 T8988 T8987 compound X,inactivation
R5453 T8989 T8987 punct -,inactivation
R5454 T8990 T8991 punct (,M.
R5455 T8991 T8935 meta M.,provided
R5456 T8992 T8991 nmod Muers,M.
R5457 T8993 T8991 punct ", ",M.
R5458 T8994 T8991 amod personal,M.
R5459 T8995 T8991 nmod communication,M.
R546 T1034 T1032 punct ",",5
R5460 T8996 T8991 punct ),M.
R5461 T8997 T8935 punct .,provided
R5462 T8999 T9000 nsubj Atrx,joins
R5463 T9001 T9002 det an,list
R5464 T9002 T9000 dobj list,joins
R5465 T9003 T9002 amod expanding,list
R5466 T9004 T9002 prep of,list
R5467 T9005 T9006 compound mouse,genes
R5468 T9006 T9004 pobj genes,of
R5469 T9007 T9008 prep for,results
R547 T1035 T1032 punct ],5
R5470 T9008 T9002 relcl results,list
R5471 T9009 T9007 pobj which,for
R5472 T9010 T9011 amod targeted,disruption
R5473 T9011 T9008 nsubj disruption,results
R5474 T9012 T9008 prep in,results
R5475 T9013 T9014 amod peri-implantation,lethality
R5476 T9014 T9012 pobj lethality,in
R5477 T9015 T9008 prep as,results
R5478 T9016 T9017 det a,result
R5479 T9017 T9015 pobj result,as
R548 T1036 T991 punct .,situated
R5480 T9018 T9017 prep of,result
R5481 T9019 T9020 nmod trophoblast,abnormalities
R5482 T9020 T9018 pobj abnormalities,of
R5483 T9021 T9019 cc or,trophoblast
R5484 T9022 T9019 conj placental,trophoblast
R5485 T9023 T9024 punct (,reviewed
R5486 T9024 T9000 parataxis reviewed,joins
R5487 T9025 T9024 prep in,reviewed
R5488 T9026 T9025 punct [,in
R5489 T9027 T9025 pobj 25,in
R549 T1038 T1039 npadvmod Disease,causing
R5490 T9028 T9024 punct ],reviewed
R5491 T9029 T9024 punct ),reviewed
R5492 T9030 T9000 punct .,joins
R5493 T9032 T9033 nsubj Comparison,provide
R5494 T9034 T9032 prep with,Comparison
R5495 T9035 T9036 amod other,phenotypes
R5496 T9036 T9034 pobj phenotypes,with
R5497 T9037 T9033 aux might,provide
R5498 T9038 T9039 det some,insight
R5499 T9039 T9033 dobj insight,provide
R550 T1039 T1041 amod causing,mutations
R5500 T9040 T9033 prep into,provide
R5501 T9041 T9042 det the,role
R5502 T9042 T9040 pobj role,into
R5503 T9043 T9042 prep of,role
R5504 T9044 T9043 pobj Atrx,of
R5505 T9045 T9042 prep in,role
R5506 T9046 T9047 compound trophoblast,development
R5507 T9047 T9045 pobj development,in
R5508 T9048 T9033 punct .,provide
R5509 T9050 T9051 compound Atrx,mutant
R551 T1040 T1039 punct -,causing
R5510 T9051 T9053 compound mutant,embryos
R5511 T9052 T9051 punct -,mutant
R5512 T9053 T9054 nsubj embryos,progress
R5513 T9055 T9054 advmod further,progress
R5514 T9056 T9055 prep than,further
R5515 T9057 T9056 pobj embryos,than
R5516 T9058 T9057 amod nullizygous,embryos
R5517 T9059 T9058 prep for,nullizygous
R5518 T9060 T9059 pobj factors,for
R5519 T9061 T9060 acl involved,factors
R552 T1041 T1043 nsubjpass mutations,clustered
R5520 T9062 T9061 prep in,involved
R5521 T9063 T9064 det the,specification
R5522 T9064 T9062 pobj specification,in
R5523 T9065 T9064 amod initial,specification
R5524 T9066 T9064 prep of,specification
R5525 T9067 T9068 compound trophoblast,cells
R5526 T9068 T9066 pobj cells,of
R5527 T9069 T9068 compound stem,cells
R5528 T9070 T9071 punct (,as
R5529 T9071 T9064 parataxis as,specification
R553 T1042 T1041 compound missense,mutations
R5530 T9072 T9071 amod such,as
R5531 T9073 T9071 pobj Cdx2,as
R5532 T9074 T9071 punct ),as
R5533 T9075 T9062 cc or,in
R5534 T9076 T9062 conj in,in
R5535 T9077 T9078 compound stem,maintenance
R5536 T9078 T9076 pobj maintenance,in
R5537 T9079 T9078 compound cell,maintenance
R5538 T9080 T9078 cc and,maintenance
R5539 T9081 T9078 conj proliferation,maintenance
R554 T1044 T1043 auxpass are,clustered
R5540 T9082 T9083 punct (,as
R5541 T9083 T9078 parataxis as,maintenance
R5542 T9084 T9083 amod such,as
R5543 T9085 T9083 pobj Eomes,as
R5544 T9086 T9083 punct ),as
R5545 T9087 T9054 punct .,progress
R5546 T9089 T9090 compound Cdx2,mutant
R5547 T9090 T9092 compound mutant,embryos
R5548 T9091 T9090 punct -,mutant
R5549 T9092 T9093 nsubj embryos,fail
R555 T1045 T1043 prep in,clustered
R5550 T9094 T9095 aux to,implant
R5551 T9095 T9093 xcomp implant,fail
R5552 T9096 T9093 cc and,fail
R5553 T9097 T9093 conj die,fail
R5554 T9098 T9099 quantmod between,3.5
R5555 T9099 T9100 nummod 3.5,dpc
R5556 T9100 T9097 dobj dpc,die
R5557 T9101 T9099 cc and,3.5
R5558 T9102 T9099 conj 5.5,3.5
R5559 T9103 T9104 punct [,26
R556 T1046 T1047 nummod two,regions
R5560 T9104 T9093 parataxis 26,fail
R5561 T9105 T9104 punct ],26
R5562 T9106 T9093 punct ", ",fail
R5563 T9107 T9108 mark while,implant
R5564 T9108 T9093 advcl implant,fail
R5565 T9109 T9110 compound Eomes,mutant
R5566 T9110 T9112 compound mutant,blastocysts
R5567 T9111 T9110 punct -,mutant
R5568 T9112 T9108 nsubj blastocysts,implant
R5569 T9113 T9108 prep into,implant
R557 T1047 T1045 pobj regions,in
R5570 T9114 T9115 det the,uterus
R5571 T9115 T9113 pobj uterus,into
R5572 T9116 T9108 punct ", ",implant
R5573 T9117 T9108 cc but,implant
R5574 T9118 T9108 conj arrest,implant
R5575 T9119 T9120 advmod soon,after
R5576 T9120 T9118 prep after,arrest
R5577 T9121 T9120 pobj implantation,after
R5578 T9122 T9118 prep without,arrest
R5579 T9123 T9122 pcomp forming,without
R558 T1048 T1047 prep of,regions
R5580 T9124 T9125 amod organised,structures
R5581 T9125 T9123 dobj structures,forming
R5582 T9126 T9125 amod embryonic,structures
R5583 T9127 T9126 cc or,embryonic
R5584 T9128 T9126 conj extraembryonic,embryonic
R5585 T9129 T9130 punct [,27
R5586 T9130 T9093 parataxis 27,fail
R5587 T9131 T9130 punct ],27
R5588 T9132 T9093 punct .,fail
R5589 T9134 T9135 prep In,implant
R559 T1049 T1050 det the,gene
R5590 T9136 T9134 pobj contrast,In
R5591 T9137 T9135 punct ", ",implant
R5592 T9138 T9139 compound Atrx,mutant
R5593 T9139 T9141 compound mutant,embryos
R5594 T9140 T9139 punct -,mutant
R5595 T9141 T9135 nsubj embryos,implant
R5596 T9142 T9135 advmod successfully,implant
R5597 T9143 T9135 cc and,implant
R5598 T9144 T9135 conj establish,implant
R5599 T9145 T9146 amod organised,structures
R560 T1050 T1048 pobj gene,of
R5600 T9146 T9144 dobj structures,establish
R5601 T9147 T9146 amod embryonic,structures
R5602 T9148 T9144 prep by,establish
R5603 T9149 T9150 nummod 7.5,dpc
R5604 T9150 T9148 pobj dpc,by
R5605 T9151 T9135 punct .,implant
R5606 T9153 T9154 det The,phenotype
R5607 T9154 T9158 nsubj phenotype,resembles
R5608 T9155 T9156 compound Atrx,mutant
R5609 T9156 T9154 compound mutant,phenotype
R561 T1051 T1047 punct : ,regions
R5610 T9157 T9156 punct -,mutant
R5611 T9159 T9158 advmod closely,resembles
R5612 T9160 T9158 dobj that,resembles
R5613 T9161 T9160 acl observed,that
R5614 T9162 T9161 prep in,observed
R5615 T9163 T9162 pobj mice,in
R5616 T9164 T9163 amod nullizygous,mice
R5617 T9165 T9164 prep for,nullizygous
R5618 T9166 T9167 det the,factor
R5619 T9167 T9165 pobj factor,for
R562 T1052 T1053 det a,domain
R5620 T9168 T9167 amod basic,factor
R5621 T9169 T9170 compound helix,helix
R5622 T9170 T9167 compound helix,factor
R5623 T9171 T9170 punct -,helix
R5624 T9172 T9170 compound loop,helix
R5625 T9173 T9170 punct -,helix
R5626 T9174 T9167 compound transcription,factor
R5627 T9175 T9167 appos Hand1,factor
R5628 T9176 T9158 punct .,resembles
R5629 T9178 T9179 compound Hand1,mutant
R563 T1053 T1047 appos domain,regions
R5630 T9179 T9181 compound mutant,conceptuses
R5631 T9180 T9179 punct -,mutant
R5632 T9181 T9182 nsubj conceptuses,arrest
R5633 T9183 T9182 prep at,arrest
R5634 T9184 T9185 quantmod around,7.5
R5635 T9185 T9186 nummod 7.5,dpc
R5636 T9186 T9183 pobj dpc,at
R5637 T9187 T9182 cc and,arrest
R5638 T9188 T9182 conj display,arrest
R5639 T9189 T9190 det a,compartment
R564 T1054 T1055 npadvmod PHD,like
R5640 T9190 T9188 dobj compartment,display
R5641 T9191 T9190 amod normal,compartment
R5642 T9192 T9190 amod embryonic,compartment
R5643 T9193 T9182 punct ", ",arrest
R5644 T9194 T9182 cc but,arrest
R5645 T9195 T9182 punct ", ",arrest
R5646 T9196 T9197 prep like,causes
R5647 T9197 T9182 conj causes,arrest
R5648 T9198 T9199 compound Atrx,mutant
R5649 T9199 T9201 compound mutant,embryos
R565 T1055 T1053 amod like,domain
R5650 T9200 T9199 punct -,mutant
R5651 T9201 T9196 pobj embryos,like
R5652 T9202 T9197 punct ", ",causes
R5653 T9203 T9197 nsubj ablation,causes
R5654 T9204 T9203 prep of,ablation
R5655 T9205 T9204 pobj Hand1,of
R5656 T9206 T9207 det a,reduction
R5657 T9207 T9197 dobj reduction,causes
R5658 T9208 T9207 prep in,reduction
R5659 T9209 T9210 det the,size
R566 T1056 T1055 punct -,like
R5660 T9210 T9208 pobj size,in
R5661 T9211 T9210 prep of,size
R5662 T9212 T9213 det the,cone
R5663 T9213 T9211 pobj cone,of
R5664 T9214 T9213 amod ectoplacental,cone
R5665 T9215 T9210 cc and,size
R5666 T9216 T9210 conj density,size
R5667 T9217 T9216 prep of,density
R5668 T9218 T9217 pobj TGCs,of
R5669 T9219 T9220 punct [,28
R567 T1057 T1053 compound zinc,domain
R5670 T9220 T9197 parataxis 28,causes
R5671 T9221 T9220 punct ],28
R5672 T9222 T9197 punct .,causes
R5673 T9224 T9225 prep As,recovered
R5674 T9226 T9224 prep with,As
R5675 T9227 T9228 compound Atrx,mutants
R5676 T9228 T9226 pobj mutants,with
R5677 T9229 T9225 punct ", ",recovered
R5678 T9230 T9231 advmod only,conceptuses
R5679 T9231 T9225 nsubjpass conceptuses,recovered
R568 T1058 T1053 compound finger,domain
R5680 T9232 T9231 amod arrested,conceptuses
R5681 T9233 T9232 cc or,arrested
R5682 T9234 T9232 conj resorbed,arrested
R5683 T9235 T9236 compound Hand1,mutant
R5684 T9236 T9231 compound mutant,conceptuses
R5685 T9237 T9236 punct -,mutant
R5686 T9238 T9225 auxpass were,recovered
R5687 T9239 T9225 prep after,recovered
R5688 T9240 T9241 nummod 8.5,dpc
R5689 T9241 T9239 pobj dpc,after
R569 T1059 T1053 cc and,domain
R5690 T9242 T9225 punct .,recovered
R5691 T9244 T9245 advmod Also,affects
R5692 T9246 T9245 prep like,affects
R5693 T9247 T9246 pobj Atrx,like
R5694 T9248 T9245 punct ", ",affects
R5695 T9249 T9245 nsubj disruption,affects
R5696 T9250 T9249 prep of,disruption
R5697 T9251 T9250 pobj Hand1,of
R5698 T9252 T9245 advmod specifically,affects
R5699 T9253 T9254 amod secondary,cell
R570 T1060 T1061 det a,domain
R5700 T9254 T9256 compound cell,formation
R5701 T9255 T9254 amod giant,cell
R5702 T9256 T9245 dobj formation,affects
R5703 T9257 T9245 punct ", ",affects
R5704 T9258 T9245 cc and,affects
R5705 T9259 T9260 amod primary,outgrowths
R5706 T9260 T9262 nsubj outgrowths,appeared
R5707 T9261 T9260 compound trophoblast,outgrowths
R5708 T9262 T9245 conj appeared,affects
R5709 T9263 T9260 prep from,outgrowths
R571 T1061 T1053 conj domain,domain
R5710 T9264 T9263 pobj blastocysts,from
R5711 T9265 T9262 oprd normal,appeared
R5712 T9266 T9262 punct .,appeared
R5713 T9268 T9269 nsubjpass Hand1,required
R5714 T9270 T9269 auxpass is,required
R5715 T9271 T9269 prep for,required
R5716 T9272 T9273 amod terminal,differentiation
R5717 T9273 T9271 pobj differentiation,for
R5718 T9274 T9273 prep of,differentiation
R5719 T9275 T9276 amod secondary,TGCs
R572 T1062 T1063 npadvmod SNF2,like
R5720 T9276 T9274 pobj TGCs,of
R5721 T9277 T9269 punct ", ",required
R5722 T9278 T9269 cc and,required
R5723 T9279 T9280 prep in,arrest
R5724 T9280 T9269 conj arrest,required
R5725 T9281 T9282 poss its,absence
R5726 T9282 T9279 pobj absence,in
R5727 T9283 T9284 compound trophoblast,cells
R5728 T9284 T9280 nsubj cells,arrest
R5729 T9285 T9280 prep at,arrest
R573 T1063 T1061 amod like,domain
R5730 T9286 T9287 det a,stage
R5731 T9287 T9285 pobj stage,at
R5732 T9288 T9287 compound precursor,stage
R5733 T9289 T9280 prep in,arrest
R5734 T9290 T9291 det the,cone
R5735 T9291 T9289 pobj cone,in
R5736 T9292 T9291 amod ectoplacental,cone
R5737 T9293 T9294 punct [,28
R5738 T9294 T9280 parataxis 28,arrest
R5739 T9295 T9294 nummod 17,28
R574 T1064 T1063 punct -,like
R5740 T9296 T9294 punct ",",28
R5741 T9297 T9294 punct ],28
R5742 T9298 T9280 punct .,arrest
R5743 T9300 T9301 prep Given,be
R5744 T9302 T9303 det the,similarity
R5745 T9303 T9300 pobj similarity,Given
R5746 T9304 T9303 prep of,similarity
R5747 T9305 T9306 det the,phenotypes
R5748 T9306 T9304 pobj phenotypes,of
R5749 T9307 T9308 nmod Atrx,mutant
R575 T1065 T1061 compound ATPase,domain
R5750 T9308 T9306 compound mutant,phenotypes
R5751 T9309 T9307 punct -,Atrx
R5752 T9310 T9307 cc and,Atrx
R5753 T9311 T9307 conj Hand1,Atrx
R5754 T9312 T9308 punct -,mutant
R5755 T9313 T9303 cc and,similarity
R5756 T9314 T9315 det the,likelihood
R5757 T9315 T9303 conj likelihood,similarity
R5758 T9316 T9317 mark that,acts
R5759 T9317 T9315 acl acts,likelihood
R576 T1066 T1067 punct (,Figure
R5760 T9318 T9317 nsubj Atrx,acts
R5761 T9319 T9317 prep as,acts
R5762 T9320 T9321 det a,regulator
R5763 T9321 T9319 pobj regulator,as
R5764 T9322 T9321 amod transcriptional,regulator
R5765 T9323 T9317 prep by,acts
R5766 T9324 T9323 pcomp modifying,by
R5767 T9325 T9326 compound chromatin,structure
R5768 T9326 T9324 dobj structure,modifying
R5769 T9327 T9301 punct ", ",be
R577 T1067 T1043 parataxis Figure,clustered
R5770 T9328 T9301 nsubj it,be
R5771 T9329 T9301 aux will,be
R5772 T9330 T9301 prep of,be
R5773 T9331 T9330 pobj interest,of
R5774 T9332 T9333 aux to,determine
R5775 T9333 T9301 xcomp determine,be
R5776 T9334 T9335 mark whether,is
R5777 T9335 T9333 advcl is,determine
R5778 T9336 T9335 nsubj Atrx,is
R5779 T9337 T9335 npadvmod itself,is
R578 T1068 T1067 nummod 1,Figure
R5780 T9338 T9339 det a,regulator
R5781 T9339 T9335 attr regulator,is
R5782 T9340 T9339 prep of,regulator
R5783 T9341 T9342 compound Hand1,expression
R5784 T9342 T9340 pobj expression,of
R5785 T9343 T9335 punct ", ",is
R5786 T9344 T9335 cc or,is
R5787 T9345 T9346 advmod alternatively,acts
R5788 T9346 T9335 conj acts,is
R5789 T9347 T9346 mark whether,acts
R579 T1069 T1067 punct ),Figure
R5790 T9348 T9346 nsubj it,acts
R5791 T9349 T9346 prep as,acts
R5792 T9350 T9351 det a,co-regulator
R5793 T9351 T9349 pobj co-regulator,as
R5794 T9352 T9351 prep of,co-regulator
R5795 T9353 T9352 pobj one,of
R5796 T9354 T9353 cc or,one
R5797 T9355 T9353 conj more,one
R5798 T9356 T9353 prep of,one
R5799 T9357 T9358 det the,targets
R580 T1070 T1071 punct [,6
R5800 T9358 T9356 pobj targets,of
R5801 T9359 T9358 amod downstream,targets
R5802 T9360 T9358 amod transcriptional,targets
R5803 T9361 T9358 prep of,targets
R5804 T9362 T9361 pobj Hand1,of
R5805 T9363 T9301 punct .,be
R5806 T9365 T9366 nsubj It,is
R5807 T9367 T9366 acomp noteworthy,is
R5808 T9368 T9369 mark that,observed
R5809 T9369 T9366 ccomp observed,is
R581 T1071 T1043 parataxis 6,clustered
R5810 T9370 T9369 punct ", ",observed
R5811 T9371 T9369 prep in,observed
R5812 T9372 T9373 det the,mice
R5813 T9373 T9371 pobj mice,in
R5814 T9374 T9375 npadvmod brain,specific
R5815 T9375 T9373 amod specific,mice
R5816 T9376 T9375 punct -,specific
R5817 T9377 T9378 compound Atrx,knockout
R5818 T9378 T9373 compound knockout,mice
R5819 T9379 T9369 punct ", ",observed
R582 T1072 T1071 punct ],6
R5820 T9380 T9381 det the,defect
R5821 T9381 T9369 nsubjpass defect,observed
R5822 T9382 T9369 auxpass was,observed
R5823 T9383 T9369 prep in,observed
R5824 T9384 T9385 advmod terminally,differentiating
R5825 T9385 T9386 amod differentiating,neurons
R5826 T9386 T9383 pobj neurons,in
R5827 T9387 T9388 punct [,20
R5828 T9388 T9366 parataxis 20,is
R5829 T9389 T9388 punct ],20
R583 T1073 T1043 punct .,clustered
R5830 T9390 T9366 punct .,is
R5831 T9392 T9393 det The,TGCs
R5832 T9393 T9395 nsubj TGCs,represent
R5833 T9394 T9393 amod secondary,TGCs
R5834 T9396 T9393 acl affected,TGCs
R5835 T9397 T9396 prep in,affected
R5836 T9398 T9399 det the,knockout
R5837 T9399 T9397 pobj knockout,in
R5838 T9400 T9399 amod universal,knockout
R5839 T9401 T9399 compound Atrx,knockout
R584 T1075 T1076 det The,motif
R5840 T9402 T9399 acl reported,knockout
R5841 T9403 T9402 advmod here,reported
R5842 T9404 T9395 dobj one,represent
R5843 T9405 T9404 prep of,one
R5844 T9406 T9407 det the,tissues
R5845 T9407 T9405 pobj tissues,of
R5846 T9408 T9407 amod first,tissues
R5847 T9409 T9410 advmod terminally,differentiated
R5848 T9410 T9407 amod differentiated,tissues
R5849 T9411 T9404 prep in,one
R585 T1076 T1078 nsubjpass motif,thought
R5850 T9412 T9413 det the,mouse
R5851 T9413 T9411 pobj mouse,in
R5852 T9414 T9413 amod developing,mouse
R5853 T9415 T9395 punct ", ",represent
R5854 T9416 T9395 cc and,represent
R5855 T9417 T9418 nsubj this,point
R5856 T9418 T9395 conj point,represent
R5857 T9419 T9418 aux may,point
R5858 T9420 T9418 prep to,point
R5859 T9421 T9422 det the,requirement
R586 T1077 T1076 amod former,motif
R5860 T9422 T9420 pobj requirement,to
R5861 T9423 T9422 prep for,requirement
R5862 T9424 T9423 pobj Atrx,for
R5863 T9425 T9422 prep in,requirement
R5864 T9426 T9427 det the,expression
R5865 T9427 T9425 pobj expression,in
R5866 T9428 T9429 amod high,level
R5867 T9429 T9427 compound level,expression
R5868 T9430 T9429 punct -,level
R5869 T9431 T9427 prep of,expression
R587 T1079 T1078 auxpass is,thought
R5870 T9432 T9433 det some,genes
R5871 T9433 T9431 pobj genes,of
R5872 T9434 T9435 npadvmod tissue,specific
R5873 T9435 T9433 amod specific,genes
R5874 T9436 T9435 punct -,specific
R5875 T9437 T9422 prep during,requirement
R5876 T9438 T9439 det the,stages
R5877 T9439 T9437 pobj stages,during
R5878 T9440 T9439 amod final,stages
R5879 T9441 T9439 prep of,stages
R588 T1080 T1081 aux to,involved
R5880 T9442 T9441 pobj differentiation,of
R5881 T9443 T9418 punct .,point
R5882 T9445 T9446 advmod Interestingly,expressed
R5883 T9447 T9446 punct ", ",expressed
R5884 T9448 T9449 det the,genes
R5885 T9449 T9446 nsubjpass genes,expressed
R5886 T9450 T9451 compound α,globin
R5887 T9451 T9449 compound globin,genes
R5888 T9452 T9451 punct -,globin
R5889 T9453 T9449 punct ", ",genes
R589 T1081 T1078 xcomp involved,thought
R5890 T9454 T9455 det the,targets
R5891 T9455 T9449 appos targets,genes
R5892 T9456 T9455 advmod only,targets
R5893 T9457 T9455 amod confirmed,targets
R5894 T9458 T9455 amod transcriptional,targets
R5895 T9459 T9455 prep of,targets
R5896 T9460 T9459 pobj regulation,of
R5897 T9461 T9460 prep by,regulation
R5898 T9462 T9463 amod human,ATRX
R5899 T9463 T9461 pobj ATRX,by
R590 T1082 T1081 auxpass be,involved
R5900 T9464 T9446 punct ", ",expressed
R5901 T9465 T9446 auxpass are,expressed
R5902 T9466 T9446 advmod also,expressed
R5903 T9467 T9446 advmod highly,expressed
R5904 T9468 T9469 advmod specifically,during
R5905 T9469 T9446 prep during,expressed
R5906 T9470 T9471 amod terminal,differentiation
R5907 T9471 T9469 pobj differentiation,during
R5908 T9472 T9446 prep within,expressed
R5909 T9473 T9474 det the,lineage
R591 T1083 T1081 prep in,involved
R5910 T9474 T9472 pobj lineage,within
R5911 T9475 T9474 amod erythroid,lineage
R5912 T9476 T9446 punct .,expressed
R592 T1084 T1085 nmod protein,protein
R5927 T9932 T9933 nsubj Atrx,Escapes
R5928 T9934 T9935 amod Imprinted,Inactivation
R5929 T9935 T9933 dobj Inactivation,Escapes
R593 T1085 T1087 compound protein,interactions
R5930 T9936 T9935 compound X,Inactivation
R5931 T9937 T9935 punct -,Inactivation
R5932 T9938 T9933 prep in,Escapes
R5933 T9939 T9940 amod Extraembryonic,Tissues
R5934 T9940 T9938 pobj Tissues,in
R5935 T9941 T9940 prep of,Tissues
R5936 T9942 T9943 nmod Carrier,Mice
R5937 T9943 T9941 pobj Mice,of
R5938 T9944 T9943 amod Female,Mice
R5939 T9946 T9947 det Another,finding
R594 T1086 T1085 punct -,protein
R5940 T9947 T9949 nsubj finding,is
R5941 T9948 T9947 amod surprising,finding
R5942 T9950 T9947 prep of,finding
R5943 T9951 T9952 det this,study
R5944 T9952 T9950 pobj study,of
R5945 T9953 T9954 mark that,appears
R5946 T9954 T9949 ccomp appears,is
R5947 T9955 T9954 punct ", ",appears
R5948 T9956 T9954 prep in,appears
R5949 T9957 T9958 nmod carrier,embryos
R595 T1087 T1083 pobj interactions,in
R5950 T9958 T9956 pobj embryos,in
R5951 T9959 T9958 amod female,embryos
R5952 T9960 T9954 punct ", ",appears
R5953 T9961 T9962 det a,allele
R5954 T9962 T9954 nsubj allele,appears
R5955 T9963 T9964 advmod paternally,inherited
R5956 T9964 T9962 amod inherited,allele
R5957 T9965 T9962 compound Atrx WT,allele
R5958 T9966 T9967 aux to,escape
R5959 T9967 T9954 xcomp escape,appears
R596 T1088 T1087 prep in,interactions
R5960 T9968 T9969 det the,process
R5961 T9969 T9967 dobj process,escape
R5962 T9970 T9969 prep of,process
R5963 T9971 T9972 amod imprinted,inactivation
R5964 T9972 T9970 pobj inactivation,of
R5965 T9973 T9972 compound X,inactivation
R5966 T9974 T9972 punct -,inactivation
R5967 T9975 T9969 punct ", ",process
R5968 T9976 T9977 dep which,silences
R5969 T9977 T9969 relcl silences,process
R597 T1089 T1088 pobj chromatin,in
R5970 T9978 T9977 advmod ordinarily,silences
R5971 T9979 T9980 det the,chromosome
R5972 T9980 T9977 dobj chromosome,silences
R5973 T9981 T9980 compound Xp,chromosome
R5974 T9982 T9977 prep in,silences
R5975 T9983 T9984 det the,compartment
R5976 T9984 T9982 pobj compartment,in
R5977 T9985 T9984 amod extraembryonic,compartment
R5978 T9986 T9984 prep of,compartment
R5979 T9987 T9988 amod female,tissues
R598 T1090 T1091 punct [,7
R5980 T9988 T9986 pobj tissues,of
R5981 T9989 T9988 amod murine,tissues
R5982 T9990 T9991 punct [,18
R5983 T9991 T9949 parataxis 18,is
R5984 T9992 T9991 punct ],18
R5985 T9993 T9949 punct .,is
R5986 T9995 T9996 nsubj Silencing,render
R5987 T9997 T9995 prep of,Silencing
R5988 T9998 T9999 det the,allele
R5989 T9999 T9997 pobj allele,of
R599 T1091 T1078 parataxis 7,thought
R5990 T10000 T9999 compound Atrx WT,allele
R5991 T10001 T9995 prep on,Silencing
R5992 T10002 T10001 pobj Xp,on
R5993 T10003 T9996 aux should,render
R5994 T10004 T10005 det these,females
R5995 T10005 T9996 dobj females,render
R5996 T10006 T9996 oprd null,render
R5997 T10007 T10006 prep for,null
R5998 T10008 T10007 pobj Atrx,for
R5999 T10009 T9996 prep in,render
R600 T1092 T1091 punct ],7
R6000 T10010 T10011 det the,tissues
R6001 T10011 T10009 pobj tissues,in
R6002 T10012 T10011 amod extraembryonic,tissues
R6003 T10013 T9996 punct ", ",render
R6004 T10014 T10015 mark since,carries
R6005 T10015 T9996 advcl carries,render
R6006 T10016 T10017 det the,chromosome
R6007 T10017 T10015 nsubj chromosome,carries
R6008 T10018 T10019 advmod normally,active
R6009 T10019 T10017 amod active,chromosome
R601 T1093 T1078 punct ", ",thought
R6010 T10020 T10017 compound Xm,chromosome
R6011 T10021 T10022 det the,allele
R6012 T10022 T10015 dobj allele,carries
R6013 T10023 T10022 compound Atrx Δ18Δneo,allele
R6014 T10024 T9996 punct .,render
R6015 T10026 T10027 mark Although,normal
R6016 T10027 T10030 advcl normal,developed
R6017 T10028 T10027 neg not,normal
R6018 T10029 T10027 advmod phenotypically,normal
R6019 T10031 T10030 punct ", ",developed
R602 T1094 T1078 cc and,thought
R6020 T10032 T10033 det some,females
R6021 T10033 T10030 nsubj females,developed
R6022 T10034 T10033 compound Atrx,females
R6023 T10035 T10033 compound carrier,females
R6024 T10036 T10030 prep to,developed
R6025 T10037 T10036 pobj term,to
R6026 T10038 T10030 cc and,developed
R6027 T10039 T10030 conj went,developed
R6028 T10040 T10039 prt on,went
R6029 T10041 T10042 aux to,reproduce
R603 T1095 T1096 det the,latter
R6030 T10042 T10039 xcomp reproduce,went
R6031 T10043 T10030 punct .,developed
R6032 T10045 T10046 advmod Thus,is
R6033 T10047 T10046 punct ", ",is
R6034 T10048 T10049 det the,failure
R6035 T10049 T10046 nsubj failure,is
R6036 T10050 T10051 aux to,silence
R6037 T10051 T10049 acl silence,failure
R6038 T10052 T10051 advmod correctly,silence
R6039 T10053 T10054 det the,allele
R604 T1096 T1097 nsubj latter,is
R6040 T10054 T10051 dobj allele,silence
R6041 T10055 T10056 advmod paternally,derived
R6042 T10056 T10054 amod derived,allele
R6043 T10057 T10054 compound Atrx WT,allele
R6044 T10058 T10054 prep in,allele
R6045 T10059 T10060 det the,tissues
R6046 T10060 T10058 pobj tissues,in
R6047 T10061 T10060 amod extraembryonic,tissues
R6048 T10062 T10060 prep of,tissues
R6049 T10063 T10064 compound carrier,females
R605 T1097 T1078 conj is,thought
R6050 T10064 T10062 pobj females,of
R6051 T10065 T10046 acomp consistent,is
R6052 T10066 T10065 prep with,consistent
R6053 T10067 T10068 poss our,observations
R6054 T10068 T10066 pobj observations,with
R6055 T10069 T10070 mark that,plays
R6056 T10070 T10068 acl plays,observations
R6057 T10071 T10070 prep in,plays
R6058 T10072 T10073 amod Atrxnull,males
R6059 T10073 T10071 pobj males,in
R606 T1098 T1099 det a,feature
R6060 T10074 T10070 punct ", ",plays
R6061 T10075 T10076 det the,protein
R6062 T10076 T10070 nsubj protein,plays
R6063 T10077 T10076 compound Atrx,protein
R6064 T10078 T10079 det an,role
R6065 T10079 T10070 dobj role,plays
R6066 T10080 T10079 amod essential,role
R6067 T10081 T10070 prep in,plays
R6068 T10082 T10083 det the,development
R6069 T10083 T10081 pobj development,in
R607 T1099 T1097 attr feature,is
R6070 T10084 T10083 prep of,development
R6071 T10085 T10086 det the,trophoblast
R6072 T10086 T10084 pobj trophoblast,of
R6073 T10087 T10070 cc and,plays
R6074 T10088 T10070 conj is,plays
R6075 T10089 T10088 acomp necessary,is
R6076 T10090 T10089 prep for,necessary
R6077 T10091 T10090 pobj survival,for
R6078 T10092 T10093 advmod in,utero
R6079 T10093 T10091 advmod utero,survival
R608 T1100 T1099 prep of,feature
R6080 T10094 T10088 prep in,is
R6081 T10095 T10096 det the,mouse
R6082 T10096 T10094 pobj mouse,in
R6083 T10097 T10046 punct .,is
R6084 T10099 T10100 det The,survival
R6085 T10100 T10101 nsubj survival,contrasts
R6086 T10102 T10100 prep of,survival
R6087 T10103 T10104 compound Atrx,females
R6088 T10104 T10102 pobj females,of
R6089 T10105 T10104 compound carrier,females
R609 T1101 T1102 npadvmod chromatin,remodelling
R6090 T10106 T10101 prep with,contrasts
R6091 T10107 T10108 det the,phenotypes
R6092 T10108 T10106 pobj phenotypes,with
R6093 T10109 T10108 acl seen,phenotypes
R6094 T10110 T10109 prep in,seen
R6095 T10111 T10110 pobj carriers,in
R6096 T10112 T10111 prep of,carriers
R6097 T10113 T10112 pobj mutations,of
R6098 T10114 T10113 prep of,mutations
R6099 T10115 T10116 amod other,genes
R610 T1102 T1104 amod remodelling,proteins
R6100 T10116 T10114 pobj genes,of
R6101 T10117 T10116 amod murine,genes
R6102 T10118 T10119 npadvmod X,linked
R6103 T10119 T10116 amod linked,genes
R6104 T10120 T10119 punct -,linked
R6105 T10121 T10116 acl known,genes
R6106 T10122 T10123 aux to,be
R6107 T10123 T10121 xcomp be,known
R6108 T10124 T10123 acomp essential,be
R6109 T10125 T10123 prep in,be
R611 T1103 T1102 punct -,remodelling
R6110 T10126 T10127 det the,compartment
R6111 T10127 T10125 pobj compartment,in
R6112 T10128 T10127 amod extraembryonic,compartment
R6113 T10129 T10101 punct .,contrasts
R6114 T10131 T10132 prep For,cause
R6115 T10133 T10131 pobj example,For
R6116 T10134 T10132 punct ", ",cause
R6117 T10135 T10136 amod targeted,disruption
R6118 T10136 T10132 nsubj disruption,cause
R6119 T10137 T10136 prep of,disruption
R612 T1104 T1100 pobj proteins,of
R6120 T10138 T10139 det the,genes
R6121 T10139 T10137 pobj genes,of
R6122 T10140 T10139 nmod dyskerin,genes
R6123 T10141 T10140 punct (,dyskerin
R6124 T10142 T10140 appos Dkc1,dyskerin
R6125 T10143 T10140 punct ),dyskerin
R6126 T10144 T10140 punct ", ",dyskerin
R6127 T10145 T10146 nmod glucose,phosphate
R6128 T10146 T10149 compound phosphate,dehydrogenase
R6129 T10147 T10146 nummod 6,phosphate
R613 T1105 T1078 punct ", ",thought
R6130 T10148 T10146 punct -,phosphate
R6131 T10149 T10140 conj dehydrogenase,dyskerin
R6132 T10150 T10149 punct (,dehydrogenase
R6133 T10151 T10149 appos G6PD,dehydrogenase
R6134 T10152 T10149 punct ),dehydrogenase
R6135 T10153 T10149 punct ", ",dehydrogenase
R6136 T10154 T10149 cc and,dehydrogenase
R6137 T10155 T10149 conj choroideremia,dehydrogenase
R6138 T10156 T10155 punct (,choroideremia
R6139 T10157 T10155 appos Chm,choroideremia
R614 T1106 T1078 cc and,thought
R6140 T10158 T10139 punct ),genes
R6141 T10159 T10160 amod embryonic,lethality
R6142 T10160 T10132 dobj lethality,cause
R6143 T10161 T10160 prep in,lethality
R6144 T10162 T10163 amod null,embryos
R6145 T10163 T10161 pobj embryos,in
R6146 T10164 T10163 amod male,embryos
R6147 T10165 T10132 prep through,cause
R6148 T10166 T10165 pobj defects,through
R6149 T10167 T10166 prep of,defects
R615 T1107 T1108 det the,presence
R6150 T10168 T10169 det the,tissues
R6151 T10169 T10167 pobj tissues,of
R6152 T10170 T10171 amod extraembryonic,derived
R6153 T10171 T10169 amod derived,tissues
R6154 T10172 T10171 punct -,derived
R6155 T10173 T10174 punct [,29
R6156 T10174 T10132 parataxis 29,cause
R6157 T10175 T10176 punct –,31
R6158 T10176 T10174 prep 31,29
R6159 T10177 T10174 punct ],29
R616 T1108 T1109 nsubj presence,indicates
R6160 T10178 T10132 punct .,cause
R6161 T10180 T10181 amod Female,mice
R6162 T10181 T10182 nsubj mice,die
R6163 T10183 T10181 acl carrying,mice
R6164 T10184 T10183 dobj mutations,carrying
R6165 T10185 T10184 prep of,mutations
R6166 T10186 T10187 det these,genes
R6167 T10187 T10185 pobj genes,of
R6168 T10188 T10184 prep on,mutations
R6169 T10189 T10190 det the,chromosome
R617 T1109 T1078 conj indicates,thought
R6170 T10190 T10188 pobj chromosome,on
R6171 T10191 T10192 advmod maternally,inherited
R6172 T10192 T10190 amod inherited,chromosome
R6173 T10193 T10190 compound X,chromosome
R6174 T10194 T10182 advmod also,die
R6175 T10195 T10196 advmod in,utero
R6176 T10196 T10182 advmod utero,die
R6177 T10197 T10182 punct ", ",die
R6178 T10198 T10199 mark whereas,survive
R6179 T10199 T10182 advcl survive,die
R618 T1110 T1108 prep of,presence
R6180 T10200 T10199 nsubj females,survive
R6181 T10201 T10202 dep that,inherit
R6182 T10202 T10200 relcl inherit,females
R6183 T10203 T10204 det the,mutation
R6184 T10204 T10202 dobj mutation,inherit
R6185 T10205 T10204 prep on,mutation
R6186 T10206 T10207 det the,chromosome
R6187 T10207 T10205 pobj chromosome,on
R6188 T10208 T10207 compound Xp,chromosome
R6189 T10209 T10182 punct .,die
R619 T1111 T1112 npadvmod disease,causing
R6190 T10211 T10212 advmod Thus,are
R6191 T10213 T10212 punct ", ",are
R6192 T10214 T10212 prep unlike,are
R6193 T10215 T10214 pobj Atrx,unlike
R6194 T10216 T10212 punct ", ",are
R6195 T10217 T10218 det these,genes
R6196 T10218 T10212 nsubj genes,are
R6197 T10219 T10218 cc and,genes
R6198 T10220 T10219 punct /,and
R6199 T10221 T10219 cc or,and
R620 T1112 T1114 amod causing,mutations
R6200 T10222 T10223 poss their,effects
R6201 T10223 T10218 conj effects,genes
R6202 T10224 T10223 prep on,effects
R6203 T10225 T10226 compound cell,growth
R6204 T10226 T10224 pobj growth,on
R6205 T10227 T10212 acomp unable,are
R6206 T10228 T10229 aux to,circumvent
R6207 T10229 T10227 xcomp circumvent,unable
R6208 T10230 T10231 det the,processes
R6209 T10231 T10229 dobj processes,circumvent
R621 T1113 T1112 punct -,causing
R6210 T10232 T10233 dep that,cause
R6211 T10233 T10231 relcl cause,processes
R6212 T10234 T10233 advmod ultimately,cause
R6213 T10235 T10236 det all,cells
R6214 T10236 T10237 nsubj cells,express
R6215 T10237 T10233 ccomp express,cause
R6216 T10238 T10236 prep in,cells
R6217 T10239 T10240 det the,tissues
R6218 T10240 T10238 pobj tissues,in
R6219 T10241 T10240 amod extraembryonic,tissues
R622 T1114 T1110 pobj mutations,of
R6220 T10242 T10237 aux to,express
R6221 T10243 T10244 advmod only,chromosome
R6222 T10244 T10237 dobj chromosome,express
R6223 T10245 T10244 det the,chromosome
R6224 T10246 T10247 advmod maternally,derived
R6225 T10247 T10244 amod derived,chromosome
R6226 T10248 T10244 compound X,chromosome
R6227 T10249 T10212 punct .,are
R6228 T10251 T10252 advmod How,maintained
R6229 T10253 T10252 aux might,maintained
R623 T1115 T1116 det the,importance
R6230 T10254 T10252 nsubjpass expression,maintained
R6231 T10255 T10254 prep of,expression
R6232 T10256 T10257 det the,allele
R6233 T10257 T10255 pobj allele,of
R6234 T10258 T10259 amod paternal,Atrx WT
R6235 T10259 T10257 compound Atrx WT,allele
R6236 T10260 T10252 auxpass be,maintained
R6237 T10261 T10252 prep in,maintained
R6238 T10262 T10263 det the,tissues
R6239 T10263 T10261 pobj tissues,in
R624 T1116 T1109 dobj importance,indicates
R6240 T10264 T10263 amod extraembryonic,tissues
R6241 T10265 T10263 prep of,tissues
R6242 T10266 T10267 det the,females
R6243 T10267 T10265 pobj females,of
R6244 T10268 T10269 compound Atrx,carrier
R6245 T10269 T10267 compound carrier,females
R6246 T10270 T10252 punct ?,maintained
R6247 T10272 T10273 nummod One,possibility
R6248 T10273 T10274 nsubj possibility,is
R6249 T10275 T10276 mark that,is
R625 T1117 T1116 amod functional,importance
R6250 T10276 T10274 ccomp is,is
R6251 T10277 T10276 punct ", ",is
R6252 T10278 T10276 prep like,is
R6253 T10279 T10280 det some,genes
R6254 T10280 T10278 pobj genes,like
R6255 T10281 T10280 amod other,genes
R6256 T10282 T10283 npadvmod X,linked
R6257 T10283 T10280 amod linked,genes
R6258 T10284 T10283 punct -,linked
R6259 T10285 T10276 punct ", ",is
R626 T1118 T1116 prep of,importance
R6260 T10286 T10276 nsubj silencing,is
R6261 T10287 T10286 prep of,silencing
R6262 T10288 T10289 det the,gene
R6263 T10289 T10287 pobj gene,of
R6264 T10290 T10289 compound Atrx,gene
R6265 T10291 T10289 prep on,gene
R6266 T10292 T10291 pobj Xp,on
R6267 T10293 T10276 acomp incomplete,is
R6268 T10294 T10276 punct ", ",is
R6269 T10295 T10296 amod such,is
R627 T1119 T1120 det these,domains
R6270 T10296 T10276 advcl is,is
R6271 T10297 T10296 mark that,is
R6272 T10298 T10296 expl there,is
R6273 T10299 T10296 advmod always,is
R6274 T10300 T10301 det a,output
R6275 T10301 T10296 attr output,is
R6276 T10302 T10303 amod low,level
R6277 T10303 T10301 nmod level,output
R6278 T10304 T10303 punct -,level
R6279 T10305 T10301 punct ", ",output
R628 T1120 T1118 pobj domains,of
R6280 T10306 T10301 amod leaky,output
R6281 T10307 T10301 prep of,output
R6282 T10308 T10307 pobj Atrx,of
R6283 T10309 T10301 prep from,output
R6284 T10310 T10311 det a,chromosome
R6285 T10311 T10309 pobj chromosome,from
R6286 T10312 T10313 advmod normally,inactivated
R6287 T10313 T10311 amod inactivated,chromosome
R6288 T10314 T10311 compound Xp,chromosome
R6289 T10315 T10296 prep in,is
R629 T1121 T1109 punct .,indicates
R6290 T10316 T10317 amod extraembryonic,tissues
R6291 T10317 T10315 pobj tissues,in
R6292 T10318 T10274 punct .,is
R6293 T10320 T10321 advmod However,demonstrated
R6294 T10322 T10321 punct ", ",demonstrated
R6295 T10323 T10321 nsubjpass it,demonstrated
R6296 T10324 T10321 auxpass was,demonstrated
R6297 T10325 T10321 advmod recently,demonstrated
R6298 T10326 T10327 mark that,silenced
R6299 T10327 T10321 ccomp silenced,demonstrated
R630 T1123 T1124 nsubjpass ATRX,shown
R6300 T10328 T10329 det the,allele
R6301 T10329 T10327 nsubjpass allele,silenced
R6302 T10330 T10331 amod paternal,Atrx
R6303 T10331 T10329 nmod Atrx,allele
R6304 T10332 T10331 punct (,Atrx
R6305 T10333 T10331 acl called,Atrx
R6306 T10334 T10333 oprd Xnp,called
R6307 T10335 T10329 punct ),allele
R6308 T10336 T10327 auxpass is,silenced
R6309 T10337 T10327 advmod completely,silenced
R631 T1125 T1124 aux has,shown
R6310 T10338 T10327 prep in,silenced
R6311 T10339 T10340 det a,line
R6312 T10340 T10338 pobj line,in
R6313 T10341 T10340 amod normal,line
R6314 T10342 T10343 compound mouse,trophoblast
R6315 T10343 T10340 compound trophoblast,line
R6316 T10344 T10345 compound stem,cell
R6317 T10345 T10340 compound cell,line
R6318 T10346 T10347 punct [,32
R6319 T10347 T10321 parataxis 32,demonstrated
R632 T1126 T1124 auxpass been,shown
R6320 T10348 T10347 punct ],32
R6321 T10349 T10321 punct ", ",demonstrated
R6322 T10350 T10321 advcl suggesting,demonstrated
R6323 T10351 T10352 mark that,escape
R6324 T10352 T10350 ccomp escape,suggesting
R6325 T10353 T10352 nsubj Atrx,escape
R6326 T10354 T10352 aux does,escape
R6327 T10355 T10352 neg not,escape
R6328 T10356 T10352 advmod normally,escape
R6329 T10357 T10358 amod imprinted,inactivation
R633 T1127 T1128 aux to,remodel
R6330 T10358 T10352 dobj inactivation,escape
R6331 T10359 T10358 compound X,inactivation
R6332 T10360 T10358 punct -,inactivation
R6333 T10361 T10358 prep in,inactivation
R6334 T10362 T10363 det the,tissues
R6335 T10363 T10361 pobj tissues,in
R6336 T10364 T10363 amod extraembryonic,tissues
R6337 T10365 T10363 prep of,tissues
R6338 T10366 T10367 amod wild,type
R6339 T10367 T10369 compound type,females
R634 T1128 T1124 xcomp remodel,shown
R6340 T10368 T10367 punct -,type
R6341 T10369 T10365 pobj females,of
R6342 T10370 T10321 punct .,demonstrated
R6343 T10372 T10373 advmod Thus,is
R6344 T10374 T10373 punct ", ",is
R6345 T10375 T10376 det the,expression
R6346 T10376 T10373 nsubj expression,is
R6347 T10377 T10376 prep of,expression
R6348 T10378 T10379 det the,allele
R6349 T10379 T10377 pobj allele,of
R635 T1129 T1128 dobj chromatin,remodel
R6350 T10380 T10381 npadvmod Xp,linked
R6351 T10381 T10379 amod linked,allele
R6352 T10382 T10381 punct -,linked
R6353 T10383 T10379 compound Atrx WT,allele
R6354 T10384 T10385 dep that,observed
R6355 T10385 T10379 relcl observed,allele
R6356 T10386 T10385 nsubj we,observed
R6357 T10387 T10373 acomp unique,is
R6358 T10388 T10387 prep to,unique
R6359 T10389 T10390 amod female,carriers
R636 T1130 T1131 punct [,8
R6360 T10390 T10388 pobj carriers,to
R6361 T10391 T10390 prep of,carriers
R6362 T10392 T10393 det the,allele
R6363 T10393 T10391 pobj allele,of
R6364 T10394 T10393 amod Atrx null,allele
R6365 T10395 T10373 punct .,is
R6366 T10397 T10398 advmod Perhaps,stems
R6367 T10399 T10400 det a,explanation
R6368 T10400 T10398 nsubj explanation,stems
R6369 T10401 T10402 advmod more,likely
R637 T1131 T1124 parataxis 8,shown
R6370 T10402 T10400 amod likely,explanation
R6371 T10403 T10400 prep for,explanation
R6372 T10404 T10405 det this,phenomenon
R6373 T10405 T10403 pobj phenomenon,for
R6374 T10406 T10398 prep from,stems
R6375 T10407 T10408 amod experimental,observations
R6376 T10408 T10406 pobj observations,from
R6377 T10409 T10408 acl suggesting,observations
R6378 T10410 T10411 mark that,escape
R6379 T10411 T10409 ccomp escape,suggesting
R638 T1132 T1131 punct ],8
R6380 T10412 T10413 amod imprinted,inactivation
R6381 T10413 T10416 nsubjpass inactivation,imposed
R6382 T10414 T10413 compound X,inactivation
R6383 T10415 T10413 punct -,inactivation
R6384 T10416 T10411 ccomp imposed,escape
R6385 T10417 T10416 auxpass is,imposed
R6386 T10418 T10416 neg not,imposed
R6387 T10419 T10416 prep on,imposed
R6388 T10420 T10421 det all,precursors
R6389 T10421 T10419 pobj precursors,on
R639 T1133 T1124 punct .,shown
R6390 T10422 T10421 prep of,precursors
R6391 T10423 T10424 det the,tissues
R6392 T10424 T10422 pobj tissues,of
R6393 T10425 T10424 nmod mouse,tissues
R6394 T10426 T10425 amod extraembryonic,mouse
R6395 T10427 T10411 punct : ,escape
R6396 T10428 T10429 det A,subpopulation
R6397 T10429 T10411 nsubj subpopulation,escape
R6398 T10430 T10429 prep of,subpopulation
R6399 T10431 T10430 pobj cells,of
R640 T1135 T1136 nsubj It,interacts
R6400 T10432 T10411 aux may,escape
R6401 T10433 T10434 det this,process
R6402 T10434 T10411 dobj process,escape
R6403 T10435 T10411 cc and,escape
R6404 T10436 T10411 conj make,escape
R6405 T10437 T10438 det a,choice
R6406 T10438 T10436 dobj choice,make
R6407 T10439 T10438 amod random,choice
R6408 T10440 T10438 punct “,choice
R6409 T10441 T10438 punct ”,choice
R641 T1137 T1136 advmod also,interacts
R6410 T10442 T10438 prep of,choice
R6411 T10443 T10444 det which,chromosome
R6412 T10444 T10446 dep chromosome,inactivated
R6413 T10445 T10444 compound X,chromosome
R6414 T10446 T10442 pcomp inactivated,of
R6415 T10447 T10446 aux will,inactivated
R6416 T10448 T10446 auxpass be,inactivated
R6417 T10449 T10398 punct .,stems
R6418 T10451 T10452 prep On,expected
R6419 T10453 T10451 amod average,On
R642 T1138 T1136 prep with,interacts
R6420 T10454 T10452 punct ", ",expected
R6421 T10455 T10456 nummod 50,%
R6422 T10456 T10452 nsubjpass %,expected
R6423 T10457 T10456 prep of,%
R6424 T10458 T10459 det the,cells
R6425 T10459 T10457 pobj cells,of
R6426 T10460 T10456 prep in,%
R6427 T10461 T10462 det this,subpopulation
R6428 T10462 T10460 pobj subpopulation,in
R6429 T10463 T10464 advmod randomly,inactivating
R643 T1139 T1138 pobj HP1,with
R6430 T10464 T10462 amod inactivating,subpopulation
R6431 T10465 T10452 aux would,expected
R6432 T10466 T10452 auxpass be,expected
R6433 T10467 T10468 aux to,maintain
R6434 T10468 T10452 xcomp maintain,expected
R6435 T10469 T10470 det an,chromosome
R6436 T10470 T10468 dobj chromosome,maintain
R6437 T10471 T10470 amod active,chromosome
R6438 T10472 T10470 compound Xp,chromosome
R6439 T10473 T10452 punct .,expected
R644 T1140 T1136 prep at,interacts
R6440 T10475 T10476 prep In,demonstrated
R6441 T10477 T10475 pobj support,In
R6442 T10478 T10477 prep of,support
R6443 T10479 T10480 det this,hypothesis
R6444 T10480 T10478 pobj hypothesis,of
R6445 T10481 T10476 punct ", ",demonstrated
R6446 T10482 T10476 nsubjpass it,demonstrated
R6447 T10483 T10476 aux has,demonstrated
R6448 T10484 T10476 auxpass been,demonstrated
R6449 T10485 T10486 mark that,failed
R645 T1141 T1140 pobj heterochromatin,at
R6450 T10486 T10476 ccomp failed,demonstrated
R6451 T10487 T10486 nsubj expression,failed
R6452 T10488 T10487 prep of,expression
R6453 T10489 T10490 advmod paternally,transmitted
R6454 T10490 T10491 amod transmitted,lacZ
R6455 T10491 T10495 nmod lacZ,transgenes
R6456 T10492 T10493 npadvmod X,linked
R6457 T10493 T10491 amod linked,lacZ
R6458 T10494 T10493 punct -,linked
R6459 T10495 T10488 pobj transgenes,of
R646 T1142 T1143 punct [,9
R6460 T10496 T10497 punct [,34
R6461 T10497 T10491 parataxis 34,lacZ
R6462 T10498 T10497 nummod 33,34
R6463 T10499 T10497 punct ",",34
R6464 T10500 T10497 punct ],34
R6465 T10501 T10491 cc and,lacZ
R6466 T10502 T10491 conj GFP,lacZ
R6467 T10503 T10504 punct [,35
R6468 T10504 T10502 parataxis 35,GFP
R6469 T10505 T10504 punct ],35
R647 T1143 T1136 parataxis 9,interacts
R6470 T10506 T10507 aux to,silenced
R6471 T10507 T10486 xcomp silenced,failed
R6472 T10508 T10507 auxpass be,silenced
R6473 T10509 T10507 prep in,silenced
R6474 T10510 T10511 det a,subpopulation
R6475 T10511 T10509 pobj subpopulation,in
R6476 T10512 T10511 amod small,subpopulation
R6477 T10513 T10511 prep of,subpopulation
R6478 T10514 T10515 amod extraembryonic,cells
R6479 T10515 T10513 pobj cells,of
R648 T1144 T1143 punct ],9
R6480 T10516 T10476 punct .,demonstrated
R6481 T10518 T10519 advmod Further,shown
R6482 T10520 T10519 punct ", ",shown
R6483 T10521 T10519 nsubjpass it,shown
R6484 T10522 T10519 aux has,shown
R6485 T10523 T10519 auxpass been,shown
R6486 T10524 T10525 mark that,is
R6487 T10525 T10519 ccomp is,shown
R6488 T10526 T10525 prep in,is
R6489 T10527 T10528 det a,subpopulation
R649 T1145 T1136 cc and,interacts
R6490 T10528 T10526 pobj subpopulation,in
R6491 T10529 T10528 prep of,subpopulation
R6492 T10530 T10531 amod extraembryonic,cells
R6493 T10531 T10529 pobj cells,of
R6494 T10532 T10525 punct ", ",is
R6495 T10533 T10525 nsubj it,is
R6496 T10534 T10535 det the,Xm
R6497 T10535 T10525 attr Xm,is
R6498 T10536 T10537 amod rather,than
R6499 T10537 T10535 cc than,Xm
R650 T1146 T1147 auxpass is,recruited
R6500 T10538 T10539 det the,Xp
R6501 T10539 T10535 conj Xp,Xm
R6502 T10540 T10541 dep that,undergoes
R6503 T10541 T10535 relcl undergoes,Xm
R6504 T10542 T10543 amod late,replication
R6505 T10543 T10541 dobj replication,undergoes
R6506 T10544 T10543 punct ", ",replication
R6507 T10545 T10546 det a,correlate
R6508 T10546 T10543 appos correlate,replication
R6509 T10547 T10546 amod molecular,correlate
R651 T1147 T1136 conj recruited,interacts
R6510 T10548 T10546 prep of,correlate
R6511 T10549 T10550 det the,state
R6512 T10550 T10548 pobj state,of
R6513 T10551 T10550 amod inactive,state
R6514 T10552 T10553 punct [,36
R6515 T10553 T10525 parataxis 36,is
R6516 T10554 T10553 nummod 18,36
R6517 T10555 T10553 punct ",",36
R6518 T10556 T10553 punct ],36
R6519 T10557 T10519 punct .,shown
R652 T1148 T1147 prep to,recruited
R6520 T10559 T10560 mark Although,small
R6521 T10560 T10562 advcl small,expand
R6522 T10561 T10560 advmod initially,small
R6523 T10563 T10560 cc and,small
R6524 T10564 T10565 advmod quickly,diluted
R6525 T10565 T10560 conj diluted,small
R6526 T10566 T10565 prep in,diluted
R6527 T10567 T10568 amod normal,embryos
R6528 T10568 T10566 pobj embryos,in
R6529 T10569 T10562 punct ", ",expand
R653 T1149 T1150 amod promyelocytic,leukemia
R6530 T10570 T10571 det the,subpopulation
R6531 T10571 T10562 nsubj subpopulation,expand
R6532 T10572 T10571 amod cellular,subpopulation
R6533 T10573 T10574 dep that,inactivates
R6534 T10574 T10571 relcl inactivates,subpopulation
R6535 T10575 T10576 det the,chromosome
R6536 T10576 T10574 dobj chromosome,inactivates
R6537 T10577 T10576 compound Xm,chromosome
R6538 T10578 T10562 aux could,expand
R6539 T10579 T10562 advmod rapidly,expand
R654 T1150 T1151 nmod leukemia,bodies
R6540 T10580 T10581 aux to,replace
R6541 T10581 T10562 advcl replace,expand
R6542 T10582 T10583 det the,cells
R6543 T10583 T10581 dobj cells,replace
R6544 T10584 T10585 advmod normally,imprinted
R6545 T10585 T10583 amod imprinted,cells
R6546 T10586 T10583 prep in,cells
R6547 T10587 T10588 amod extraembryonic,lineages
R6548 T10588 T10586 pobj lineages,in
R6549 T10589 T10590 mark if,compromises
R655 T1151 T1148 pobj bodies,to
R6550 T10590 T10562 advcl compromises,expand
R6551 T10591 T10592 det the,silencing
R6552 T10592 T10590 nsubj silencing,compromises
R6553 T10593 T10592 amod normal,silencing
R6554 T10594 T10592 prep of,silencing
R6555 T10595 T10594 pobj Xp,of
R6556 T10596 T10597 compound cell,growth
R6557 T10597 T10590 dobj growth,compromises
R6558 T10598 T10597 cc or,growth
R6559 T10599 T10597 conj differentiation,growth
R656 T1152 T1151 amod nuclear,bodies
R6560 T10600 T10562 punct .,expand
R6561 T10602 T10603 advmod Interestingly,suggested
R6562 T10604 T10603 punct ", ",suggested
R6563 T10605 T10603 nsubjpass it,suggested
R6564 T10606 T10603 aux has,suggested
R6565 T10607 T10603 auxpass been,suggested
R6566 T10608 T10609 mark that,range
R6567 T10609 T10603 ccomp range,suggested
R6568 T10610 T10611 det the,size
R6569 T10611 T10609 nsubj size,range
R657 T1153 T1147 prep via,recruited
R6570 T10612 T10611 prep of,size
R6571 T10613 T10614 det the,population
R6572 T10614 T10612 pobj population,of
R6573 T10615 T10616 dep that,escapes
R6574 T10616 T10614 relcl escapes,population
R6575 T10617 T10616 advmod initially,escapes
R6576 T10618 T10616 dobj imprinting,escapes
R6577 T10619 T10609 aux may,range
R6578 T10620 T10609 advmod widely,range
R6579 T10621 T10609 punct (,range
R658 T1154 T1155 det an,interaction
R6580 T10622 T10609 prep from,range
R6581 T10623 T10624 nummod 0,%
R6582 T10624 T10622 pobj %,from
R6583 T10625 T10622 prep to,from
R6584 T10626 T10627 nummod 30,%
R6585 T10627 T10625 pobj %,to
R6586 T10628 T10609 punct ),range
R6587 T10629 T10609 punct ", ",range
R6588 T10630 T10631 advmod even,between
R6589 T10631 T10609 prep between,range
R659 T1155 T1153 pobj interaction,via
R6590 T10632 T10633 advmod genetically,identical
R6591 T10633 T10634 amod identical,embryos
R6592 T10634 T10631 pobj embryos,between
R6593 T10635 T10636 punct [,37
R6594 T10636 T10609 parataxis 37,range
R6595 T10637 T10636 punct ],37
R6596 T10638 T10603 punct ", ",suggested
R6597 T10639 T10603 cc and,suggested
R6598 T10640 T10641 nsubj this,account
R6599 T10641 T10603 conj account,suggested
R660 T1156 T1155 prep with,interaction
R6600 T10642 T10641 aux may,account
R6601 T10643 T10641 prep for,account
R6602 T10644 T10645 det the,phenotype
R6603 T10645 T10643 pobj phenotype,for
R6604 T10646 T10645 amod variable,phenotype
R6605 T10647 T10645 acl observed,phenotype
R6606 T10648 T10647 prep among,observed
R6607 T10649 T10648 pobj females,among
R6608 T10650 T10649 acl bearing,females
R6609 T10651 T10652 npadvmod Xm,linked
R661 T1157 T1156 pobj Daxx,with
R6610 T10652 T10654 amod linked,alleles
R6611 T10653 T10652 punct -,linked
R6612 T10654 T10650 dobj alleles,bearing
R6613 T10655 T10654 compound mutant,alleles
R6614 T10656 T10654 prep of,alleles
R6615 T10657 T10656 pobj genes,of
R6616 T10658 T10657 amod essential,genes
R6617 T10659 T10658 prep for,essential
R6618 T10660 T10661 amod normal,development
R6619 T10661 T10659 pobj development,for
R662 T1158 T1159 punct [,10
R6620 T10662 T10661 amod extraembryonic,development
R6621 T10663 T10664 punct [,38
R6622 T10664 T10641 parataxis 38,account
R6623 T10665 T10664 punct ],38
R6624 T10666 T10641 punct .,account
R6625 T10668 T10669 advcl Put,be
R6626 T10670 T10668 advmod simply,Put
R6627 T10671 T10669 punct ", ",be
R6628 T10672 T10673 compound carrier,females
R6629 T10673 T10669 nsubj females,be
R663 T1159 T1147 parataxis 10,recruited
R6630 T10674 T10673 acl bearing,females
R6631 T10675 T10676 det a,population
R6632 T10676 T10674 dobj population,bearing
R6633 T10677 T10676 amod small,population
R6634 T10678 T10676 amod initial,population
R6635 T10679 T10676 prep of,population
R6636 T10680 T10681 amod escaping,cells
R6637 T10681 T10679 pobj cells,of
R6638 T10682 T10669 aux would,be
R6639 T10683 T10684 advmod more,severely
R664 T1160 T1159 punct ],10
R6640 T10684 T10685 advmod severely,affected
R6641 T10685 T10669 acomp affected,be
R6642 T10686 T10685 prep than,affected
R6643 T10687 T10686 pobj those,than
R6644 T10688 T10687 acl bearing,those
R6645 T10689 T10690 det a,population
R6646 T10690 T10688 dobj population,bearing
R6647 T10691 T10690 amod larger,population
R6648 T10692 T10669 punct .,be
R6649 T10694 T10695 nsubj This,explain
R665 T1161 T1136 punct .,interacts
R6650 T10696 T10695 aux could,explain
R6651 T10697 T10698 advmod why,observed
R6652 T10698 T10695 ccomp observed,explain
R6653 T10699 T10698 nsubj we,observed
R6654 T10700 T10698 aux have,observed
R6655 T10701 T10702 amod significant,variation
R6656 T10702 T10698 dobj variation,observed
R6657 T10703 T10702 amod phenotypic,variation
R6658 T10704 T10702 prep among,variation
R6659 T10705 T10706 compound Atrx,carrier
R666 T1163 T1164 advmod Furthermore,leads
R6660 T10706 T10707 compound carrier,females
R6661 T10707 T10704 pobj females,among
R6662 T10708 T10698 punct ", ",observed
R6663 T10709 T10698 prep with,observed
R6664 T10710 T10711 det some,carriers
R6665 T10711 T10712 nsubj carriers,dying
R6666 T10712 T10709 pobj dying,with
R6667 T10713 T10714 advmod in,utero
R6668 T10714 T10712 advmod utero,dying
R6669 T10715 T10712 prep by,dying
R667 T1165 T1164 punct ", ",leads
R6670 T10716 T10717 nummod 9.5,dpc
R6671 T10717 T10715 pobj dpc,by
R6672 T10718 T10719 punct (,Table
R6673 T10719 T10712 parataxis Table,dying
R6674 T10720 T10719 nummod 1,Table
R6675 T10721 T10719 punct ),Table
R6676 T10722 T10712 cc and,dying
R6677 T10723 T10724 nsubj others,developing
R6678 T10724 T10712 conj developing,dying
R6679 T10725 T10724 prep to,developing
R668 T1166 T1164 nsubj disruption,leads
R6680 T10726 T10725 pobj term,to
R6681 T10727 T10695 punct .,explain
R6682 T10729 T10730 det Another,mechanism
R6683 T10730 T10732 nsubj mechanism,is
R6684 T10731 T10730 amod possible,mechanism
R6685 T10733 T10734 mark that,proceeds
R6686 T10734 T10732 ccomp proceeds,is
R6687 T10735 T10734 nsubj inactivation,proceeds
R6688 T10736 T10735 prep of,inactivation
R6689 T10737 T10738 det the,X
R669 T1167 T1166 prep of,disruption
R6690 T10738 T10736 pobj X,of
R6691 T10739 T10738 amod paternal,X
R6692 T10740 T10734 advmod normally,proceeds
R6693 T10741 T10734 prep in,proceeds
R6694 T10742 T10743 det all,cells
R6695 T10743 T10741 pobj cells,in
R6696 T10744 T10734 punct ", ",proceeds
R6697 T10745 T10734 cc but,proceeds
R6698 T10746 T10747 advmod subsequently,reactivated
R6699 T10747 T10734 conj reactivated,proceeds
R670 T1168 T1167 pobj ATRX,of
R6700 T10748 T10749 det the,gene
R6701 T10749 T10747 nsubjpass gene,reactivated
R6702 T10750 T10749 compound Atrx,gene
R6703 T10751 T10749 prep within,gene
R6704 T10752 T10753 amod individual,cells
R6705 T10753 T10751 pobj cells,within
R6706 T10754 T10747 auxpass is,reactivated
R6707 T10755 T10732 punct .,is
R6708 T10757 T10758 advmod Alternatively,escape
R6709 T10759 T10758 punct ", ",escape
R671 T1169 T1164 prep to,leads
R6710 T10760 T10758 prep in,escape
R6711 T10761 T10762 det the,absence
R6712 T10762 T10760 pobj absence,in
R6713 T10763 T10762 prep of,absence
R6714 T10764 T10763 pobj Atrx,of
R6715 T10765 T10758 punct ", ",escape
R6716 T10766 T10767 det the,allele
R6717 T10767 T10758 nsubj allele,escape
R6718 T10768 T10767 amod paternal,allele
R6719 T10769 T10758 aux may,escape
R672 T1170 T1171 amod diverse,changes
R6720 T10770 T10758 advmod partially,escape
R6721 T10771 T10772 det the,process
R6722 T10772 T10758 dobj process,escape
R6723 T10773 T10772 amod normal,process
R6724 T10774 T10772 prep of,process
R6725 T10775 T10774 pobj silencing,of
R6726 T10776 T10758 punct .,escape
R6727 T10778 T10779 prep In,inactivated
R6728 T10780 T10778 pobj both,In
R6729 T10781 T10780 prep of,both
R673 T1171 T1169 pobj changes,to
R6730 T10782 T10783 det these,cases
R6731 T10783 T10781 pobj cases,of
R6732 T10784 T10779 punct ", ",inactivated
R6733 T10785 T10786 amod other,genes
R6734 T10786 T10779 nsubjpass genes,inactivated
R6735 T10787 T10786 prep on,genes
R6736 T10788 T10789 det the,chromosome
R6737 T10789 T10787 pobj chromosome,on
R6738 T10790 T10789 amod paternal,chromosome
R6739 T10791 T10789 compound X,chromosome
R674 T1172 T1171 prep in,changes
R6740 T10792 T10779 aux must,inactivated
R6741 T10793 T10779 auxpass be,inactivated
R6742 T10794 T10779 cc and,inactivated
R6743 T10795 T10779 conj remain,inactivated
R6744 T10796 T10795 advmod so,remain
R6745 T10797 T10779 punct ", ",inactivated
R6746 T10798 T10799 mark since,causes
R6747 T10799 T10779 advcl causes,inactivated
R6748 T10800 T10801 amod blocking,inactivation
R6749 T10801 T10799 nsubj inactivation,causes
R675 T1173 T1174 compound DNA,methylation
R6750 T10802 T10801 prep of,inactivation
R6751 T10803 T10804 det the,chromosome
R6752 T10804 T10802 pobj chromosome,of
R6753 T10805 T10804 amod entire,chromosome
R6754 T10806 T10804 compound Xp,chromosome
R6755 T10807 T10808 amod embryonic,lethality
R6756 T10808 T10799 dobj lethality,causes
R6757 T10809 T10799 prep due,causes
R6758 T10810 T10809 pcomp to,due
R6759 T10811 T10812 amod biallelic,expression
R676 T1174 T1172 pobj methylation,in
R6760 T10812 T10809 pobj expression,due
R6761 T10813 T10812 prep of,expression
R6762 T10814 T10815 npadvmod X,linked
R6763 T10815 T10817 amod linked,genes
R6764 T10816 T10815 punct -,linked
R6765 T10817 T10813 pobj genes,of
R6766 T10818 T10812 prep in,expression
R6767 T10819 T10820 det the,trophoblast
R6768 T10820 T10818 pobj trophoblast,in
R6769 T10821 T10822 punct [,39
R677 T1175 T1176 punct [,11
R6770 T10822 T10779 parataxis 39,inactivated
R6771 T10823 T10822 punct ],39
R6772 T10824 T10779 punct .,inactivated
R6773 T10927 T10928 compound ATR,X
R6774 T10928 T10930 compound X,syndrome
R6775 T10929 T10928 punct -,X
R6776 T10930 T10931 nsubj syndrome,is
R6777 T10932 T10933 det the,disease
R6778 T10933 T10931 attr disease,is
R6779 T10934 T10933 amod first,disease
R678 T1176 T1164 parataxis 11,leads
R6780 T10935 T10933 amod human,disease
R6781 T10936 T10933 amod genetic,disease
R6782 T10937 T10933 acl known,disease
R6783 T10938 T10939 aux to,caused
R6784 T10939 T10937 xcomp caused,known
R6785 T10940 T10939 auxpass be,caused
R6786 T10941 T10939 agent by,caused
R6787 T10942 T10941 pobj mutations,by
R6788 T10943 T10942 prep in,mutations
R6789 T10944 T10945 det a,factor
R679 T1177 T1176 punct ],11
R6790 T10945 T10943 pobj factor,in
R6791 T10946 T10947 compound chromatin,remodelling
R6792 T10947 T10945 compound remodelling,factor
R6793 T10948 T10931 punct .,is
R6794 T10950 T10951 prep At,know
R6795 T10952 T10950 amod present,At
R6796 T10953 T10951 nsubj we,know
R6797 T10954 T10951 aux do,know
R6798 T10955 T10951 neg not,know
R6799 T10956 T10957 advmod how,influences
R680 T1178 T1164 punct .,leads
R6800 T10957 T10951 advcl influences,know
R6801 T10958 T10957 nsubj ATRX,influences
R6802 T10959 T10960 compound gene,expression
R6803 T10960 T10957 dobj expression,influences
R6804 T10961 T10957 cc or,influences
R6805 T10962 T10963 det what,effect
R6806 T10963 T10964 dep effect,has
R6807 T10964 T10957 conj has,influences
R6808 T10965 T10964 nsubj it,has
R6809 T10966 T10964 prep on,has
R681 T1180 T1181 advmod Nevertheless,remains
R6810 T10967 T10968 compound cell,behaviour
R6811 T10968 T10966 pobj behaviour,on
R6812 T10969 T10951 punct .,know
R6813 T10971 T10972 advmod Nevertheless,noted
R6814 T10973 T10972 punct ", ",noted
R6815 T10974 T10972 nsubj we,noted
R6816 T10975 T10972 aux have,noted
R6817 T10976 T10972 advmod previously,noted
R6818 T10977 T10978 mark that,are
R6819 T10978 T10972 ccomp are,noted
R682 T1182 T1181 punct ", ",remains
R6820 T10979 T10978 nsubj none,are
R6821 T10980 T10979 prep of,none
R6822 T10981 T10982 det the,mutations
R6823 T10982 T10980 pobj mutations,of
R6824 T10983 T10982 amod natural,mutations
R6825 T10984 T10982 acl causing,mutations
R6826 T10985 T10986 compound ATR,X
R6827 T10986 T10988 compound X,syndrome
R6828 T10987 T10986 punct -,X
R6829 T10988 T10984 dobj syndrome,causing
R683 T1183 T1184 det the,role
R6830 T10989 T10978 attr nulls,are
R6831 T10990 T10972 punct ", ",noted
R6832 T10991 T10992 dep which,suggests
R6833 T10992 T10972 advcl suggests,noted
R6834 T10993 T10994 mark that,plays
R6835 T10994 T10992 ccomp plays,suggests
R6836 T10995 T10994 nsubj it,plays
R6837 T10996 T10997 det a,role
R6838 T10997 T10994 dobj role,plays
R6839 T10998 T10997 amod critical,role
R684 T1184 T1181 nsubj role,remains
R6840 T10999 T10994 prep in,plays
R6841 T11000 T11001 amod normal,development
R6842 T11001 T10999 pobj development,in
R6843 T11002 T10972 punct .,noted
R6844 T11004 T11005 nsubj Results,shows
R6845 T11006 T11004 prep of,Results
R6846 T11007 T11008 amod conditional,inactivation
R6847 T11008 T11006 pobj inactivation,of
R6848 T11009 T11008 prep of,inactivation
R6849 T11010 T11009 pobj Atrx,of
R685 T1185 T1186 nsubj ATRX,plays
R6850 T11011 T11008 prep in,inactivation
R6851 T11012 T11013 det the,forebrain
R6852 T11013 T11011 pobj forebrain,in
R6853 T11014 T11013 amod developing,forebrain
R6854 T11015 T11013 compound mouse,forebrain
R6855 T11016 T11004 punct ", ",Results
R6856 T11017 T11004 prep based,Results
R6857 T11018 T11017 prep on,based
R6858 T11019 T11020 det the,allele
R6859 T11020 T11018 pobj allele,on
R686 T1186 T1184 advcl plays,role
R6860 T11021 T11020 compound Atrx flox,allele
R6861 T11022 T11020 acl described,allele
R6862 T11023 T11022 advmod here,described
R6863 T11024 T11005 punct ", ",shows
R6864 T11025 T11026 mark that,exerts
R6865 T11026 T11005 ccomp exerts,shows
R6866 T11027 T11026 nsubj Atrx,exerts
R6867 T11028 T11029 det a,effect
R6868 T11029 T11026 dobj effect,exerts
R6869 T11030 T11029 amod major,effect
R687 T1187 T1186 prep in,plays
R6870 T11031 T11026 prep on,exerts
R6871 T11032 T11033 advmod terminally,differentiating
R6872 T11033 T11034 amod differentiating,neurons
R6873 T11034 T11031 pobj neurons,on
R6874 T11035 T11005 punct .,shows
R6875 T11037 T11038 amod Conditional,inactivation
R6876 T11038 T11039 nsubj inactivation,is
R6877 T11040 T11038 prep of,inactivation
R6878 T11041 T11040 pobj Atrx,of
R6879 T11042 T11038 prep in,inactivation
R688 T1188 T1189 compound gene,expression
R6880 T11043 T11044 amod other,tissues
R6881 T11044 T11042 pobj tissues,in
R6882 T11045 T11039 acomp underway,is
R6883 T11046 T11039 punct .,is
R6884 T11048 T11049 advmod Here,shown
R6885 T11050 T11049 nsubj we,shown
R6886 T11051 T11049 aux have,shown
R6887 T11052 T11053 mark that,causes
R6888 T11053 T11049 ccomp causes,shown
R6889 T11054 T11055 npadvmod animal,wide
R689 T1189 T1187 pobj expression,in
R6890 T11055 T11057 amod wide,disruption
R6891 T11056 T11055 punct -,wide
R6892 T11057 T11053 nsubj disruption,causes
R6893 T11058 T11057 prep of,disruption
R6894 T11059 T11060 det the,gene
R6895 T11060 T11058 pobj gene,of
R6896 T11061 T11060 compound Atrx,gene
R6897 T11062 T11063 det a,phenotype
R6898 T11063 T11053 dobj phenotype,causes
R6899 T11064 T11063 amod severe,phenotype
R690 T1190 T1181 acomp unclear,remains
R6900 T11065 T11066 amod embryonic,lethal
R6901 T11066 T11063 amod lethal,phenotype
R6902 T11067 T11066 punct -,lethal
R6903 T11068 T11049 punct ", ",shown
R6904 T11069 T11049 advcl revealing,shown
R6905 T11070 T11071 det an,role
R6906 T11071 T11069 dobj role,revealing
R6907 T11072 T11071 amod essential,role
R6908 T11073 T11071 prep for,role
R6909 T11074 T11073 pobj Atrx,for
R691 T1191 T1181 punct .,remains
R6910 T11075 T11071 prep in,role
R6911 T11076 T11077 det the,formation
R6912 T11077 T11075 pobj formation,in
R6913 T11078 T11077 prep of,formation
R6914 T11079 T11080 det the,trophoblast
R6915 T11080 T11078 pobj trophoblast,of
R6916 T11081 T11080 amod murine,trophoblast
R6917 T11082 T11049 punct .,shown
R6918 T11084 T11085 prep In,appears
R6919 T11086 T11084 pobj addition,In
R692 T1193 T1194 det The,core
R6920 T11087 T11085 punct ", ",appears
R6921 T11088 T11085 nsubj Atrx,appears
R6922 T11089 T11090 aux to,escape
R6923 T11090 T11085 xcomp escape,appears
R6924 T11091 T11092 amod imprinted,chromosome
R6925 T11092 T11095 compound chromosome,inactivation
R6926 T11093 T11092 compound X,chromosome
R6927 T11094 T11092 punct -,chromosome
R6928 T11095 T11090 dobj inactivation,escape
R6929 T11096 T11090 prep in,escape
R693 T1194 T1196 nsubj core,suggests
R6930 T11097 T11098 det the,tissues
R6931 T11098 T11096 pobj tissues,in
R6932 T11099 T11098 amod extraembryonic,tissues
R6933 T11100 T11098 prep of,tissues
R6934 T11101 T11102 det some,mice
R6935 T11102 T11100 pobj mice,of
R6936 T11103 T11102 nmod carrier,mice
R6937 T11104 T11103 amod female,carrier
R6938 T11105 T11085 punct .,appears
R694 T1195 T1194 amod consistent,core
R6941 T11190 T11189 prep of,Generation
R6942 T11191 T11192 compound ES,cells
R6943 T11192 T11190 pobj cells,of
R6944 T11193 T11192 acl bearing,cells
R6945 T11194 T11195 det the,allele
R6946 T11195 T11193 dobj allele,bearing
R6947 T11196 T11195 compound Atrx flox,allele
R6948 T11197 T11189 punct .,Generation
R6949 T11199 T11200 advmod Briefly,places
R695 T1197 T1194 prep of,core
R6950 T11201 T11200 punct ", ",places
R6951 T11202 T11203 det the,vector
R6952 T11203 T11200 nsubj vector,places
R6953 T11204 T11203 compound targeting,vector
R6954 T11205 T11203 punct (,vector
R6955 T11206 T11203 acl shown,vector
R6956 T11207 T11206 prep in,shown
R6957 T11208 T11209 compound Figure,2A
R6958 T11209 T11207 pobj 2A,in
R6959 T11210 T11200 punct ),places
R696 T1198 T1199 amod clinical,features
R6960 T11211 T11212 det a,site
R6961 T11212 T11200 dobj site,places
R6962 T11213 T11212 compound loxP,site
R6963 T11214 T11200 prep within,places
R6964 T11215 T11214 pobj intron,within
R6965 T11216 T11215 nummod 18,intron
R6966 T11217 T11200 cc and,places
R6967 T11218 T11219 det a,cassette
R6968 T11219 T11200 conj cassette,places
R6969 T11220 T11221 npadvmod loxP,flanked
R697 T1199 T1197 pobj features,of
R6970 T11221 T11219 amod flanked,cassette
R6971 T11222 T11221 punct -,flanked
R6972 T11223 T11224 compound MC1neopA,selection
R6973 T11224 T11219 compound selection,cassette
R6974 T11225 T11219 prep in,cassette
R6975 T11226 T11225 pobj intron,in
R6976 T11227 T11226 nummod 17,intron
R6977 T11228 T11226 prep of,intron
R6978 T11229 T11230 det the,gene
R6979 T11230 T11228 pobj gene,of
R698 T1200 T1198 cc and,clinical
R6980 T11231 T11230 compound Atrx,gene
R6981 T11232 T11200 punct .,places
R6982 T11234 T11235 det A,description
R6983 T11235 T11237 nsubjpass description,provided
R6984 T11236 T11235 amod detailed,description
R6985 T11238 T11235 prep of,description
R6986 T11239 T11240 det the,construct
R6987 T11240 T11238 pobj construct,of
R6988 T11241 T11240 compound targeting,construct
R6989 T11242 T11237 auxpass is,provided
R699 T1201 T1198 conj haematological,clinical
R6990 T11243 T11237 prep in,provided
R6991 T11244 T11243 punct [,in
R6992 T11245 T11243 pobj 20,in
R6993 T11246 T11237 punct ],provided
R6994 T11247 T11237 punct .,provided
R6995 T11249 T11250 amod Linearised,plasmid
R6996 T11250 T11251 nsubjpass plasmid,electroporated
R6997 T11252 T11253 punct (,μg
R6998 T11253 T11250 parataxis μg,plasmid
R6999 T11254 T11253 nummod 150,μg
R700 T1202 T1194 acl observed,core
R7000 T11255 T11253 punct ),μg
R7001 T11256 T11251 auxpass was,electroporated
R7002 T11257 T11251 prep into,electroporated
R7003 T11258 T11259 quantmod 1,108
R7004 T11259 T11261 nummod 108,cells
R7005 T11260 T11259 punct ×,108
R7006 T11261 T11257 pobj cells,into
R7007 T11262 T11261 compound E14Tg2a,cells
R7008 T11263 T11261 compound ES,cells
R7009 T11264 T11251 punct ", ",electroporated
R701 T1203 T1202 prep in,observed
R7010 T11265 T11251 cc and,electroporated
R7011 T11266 T11267 nsubjpass colonies,isolated
R7012 T11267 T11251 conj isolated,electroporated
R7013 T11268 T11266 amod resistant,colonies
R7014 T11269 T11268 prep to,resistant
R7015 T11270 T11269 pobj G418,to
R7016 T11271 T11270 cc and,G418
R7017 T11272 T11270 conj ganciclovir,G418
R7018 T11273 T11267 auxpass were,isolated
R7019 T11274 T11267 punct .,isolated
R702 T1204 T1205 compound ATR,X
R7020 T11276 T11277 amod Homologous,events
R7021 T11277 T11279 nsubjpass events,identified
R7022 T11278 T11277 compound targeting,events
R7023 T11280 T11279 auxpass were,identified
R7024 T11281 T11279 prep by,identified
R7025 T11282 T11283 compound Southern,blot
R7026 T11283 T11281 pobj blot,by
R7027 T11284 T11283 prep of,blot
R7028 T11285 T11286 npadvmod EcoRI,digested
R7029 T11286 T11288 amod digested,DNA
R703 T1205 T1207 compound X,patients
R7030 T11287 T11286 punct -,digested
R7031 T11288 T11284 pobj DNA,of
R7032 T11289 T11283 cc and,blot
R7033 T11290 T11283 conj hybridisation,blot
R7034 T11291 T11290 prep with,hybridisation
R7035 T11292 T11293 det a,probe
R7036 T11293 T11291 pobj probe,with
R7037 T11294 T11293 nummod 5,probe
R7038 T11295 T11294 punct ′,5
R7039 T11296 T11293 punct (,probe
R704 T1206 T1205 punct -,X
R7040 T11297 T11293 acl generated,probe
R7041 T11298 T11297 prep with,generated
R7042 T11299 T11300 compound primers,PPS1.20
R7043 T11300 T11298 pobj PPS1.20,with
R7044 T11301 T11300 cc and,PPS1.20
R7045 T11302 T11300 conj PPS1.27,PPS1.20
R7046 T11303 T11293 punct ),probe
R7047 T11304 T11293 cc and,probe
R7048 T11305 T11306 det a,probe
R7049 T11306 T11293 conj probe,probe
R705 T1207 T1203 pobj patients,in
R7050 T11307 T11306 nummod 3,probe
R7051 T11308 T11307 punct ′,3
R7052 T11309 T11306 punct (,probe
R7053 T11310 T11311 det a,fragment
R7054 T11311 T11306 appos fragment,probe
R7055 T11312 T11313 nummod 0.9,kb
R7056 T11313 T11311 compound kb,fragment
R7057 T11314 T11313 punct -,kb
R7058 T11315 T11311 compound HaeIII,fragment
R7059 T11316 T11279 punct ),identified
R706 T1208 T1209 mark that,regulates
R7060 T11317 T11318 mark as,shown
R7061 T11318 T11279 advcl shown,identified
R7062 T11319 T11318 prep in,shown
R7063 T11320 T11321 compound Figure,2A
R7064 T11321 T11319 pobj 2A,in
R7065 T11322 T11321 cc and,2A
R7066 T11323 T11321 conj 2B,2A
R7067 T11324 T11279 punct .,identified
R7068 T11326 T11327 nsubj DNA,digested
R7069 T11328 T11326 prep from,DNA
R707 T1209 T1196 ccomp regulates,suggests
R7070 T11329 T11330 advmod correctly,targeted
R7071 T11330 T11331 amod targeted,clones
R7072 T11331 T11328 pobj clones,from
R7073 T11332 T11327 aux was,digested
R7074 T11333 T11327 advmod also,digested
R7075 T11334 T11327 prep with,digested
R7076 T11335 T11334 pobj SacI,with
R7077 T11336 T11327 cc and,digested
R7078 T11337 T11327 conj analysed,digested
R7079 T11338 T11337 prep by,analysed
R708 T1210 T1209 punct ", ",regulates
R7080 T11339 T11340 compound Southern,blot
R7081 T11340 T11338 pobj blot,by
R7082 T11341 T11340 cc and,blot
R7083 T11342 T11340 conj hybridisation,blot
R7084 T11343 T11342 prep with,hybridisation
R7085 T11344 T11345 det a,probe
R7086 T11345 T11343 pobj probe,with
R7087 T11346 T11345 prep from,probe
R7088 T11347 T11346 prep within,from
R7089 T11348 T11347 pobj intron,within
R709 T1211 T1209 prep like,regulates
R7090 T11349 T11348 nummod 17,intron
R7091 T11350 T11345 punct (,probe
R7092 T11351 T11352 det a,product
R7093 T11352 T11345 appos product,probe
R7094 T11353 T11352 compound PCR,product
R7095 T11354 T11352 acl generated,product
R7096 T11355 T11354 prep with,generated
R7097 T11356 T11357 compound primers,PPS1.15
R7098 T11357 T11355 pobj PPS1.15,with
R7099 T11358 T11357 cc and,PPS1.15
R710 T1212 T1213 det the,protein
R7100 T11359 T11357 conj Xnp46,PPS1.15
R7101 T11360 T11327 punct ),digested
R7102 T11361 T11362 aux to,confirm
R7103 T11362 T11327 advcl confirm,digested
R7104 T11363 T11364 mark that,included
R7105 T11364 T11362 ccomp included,confirm
R7106 T11365 T11366 det the,site
R7107 T11366 T11364 nsubjpass site,included
R7108 T11367 T11366 compound loxP,site
R7109 T11368 T11366 prep within,site
R711 T1213 T1211 pobj protein,like
R7110 T11369 T11368 pobj intron,within
R7111 T11370 T11369 nummod 18,intron
R7112 T11371 T11364 aux had,included
R7113 T11372 T11364 auxpass been,included
R7114 T11373 T11364 prep within,included
R7115 T11374 T11375 det the,region
R7116 T11375 T11373 pobj region,within
R7117 T11376 T11375 amod crossed,region
R7118 T11377 T11376 punct -,crossed
R7119 T11378 T11376 prt over,crossed
R712 T1214 T1215 nmod SWI2,SNF2
R7120 T11379 T11380 punct (,2A
R7121 T11380 T11327 parataxis 2A,digested
R7122 T11381 T11380 compound Figure,2A
R7123 T11382 T11380 cc and,2A
R7124 T11383 T11380 conj 2C,2A
R7125 T11384 T11380 punct ),2A
R7126 T11385 T11327 punct .,digested
R7127 T11387 T11388 nsubjpass Sequences,shown
R7128 T11389 T11387 prep of,Sequences
R7129 T11390 T11389 pobj primers,of
R713 T1215 T1213 nmod SNF2,protein
R7130 T11391 T11388 auxpass are,shown
R7131 T11392 T11388 prep in,shown
R7132 T11393 T11394 compound Table,S1
R7133 T11394 T11392 pobj S1,in
R7134 T11395 T11388 punct .,shown
R7135 T11569 T11570 compound Cre,recombination
R7136 T11571 T11570 punct -,recombination
R7137 T11572 T11570 cc and,recombination
R7138 T11573 T11570 conj characterisation,recombination
R7139 T11574 T11570 prep of,recombination
R714 T1216 T1215 punct /,SNF2
R7140 T11575 T11576 amod Atrxnull,cells
R7141 T11576 T11574 pobj cells,of
R7142 T11577 T11576 compound ES,cells
R7143 T11578 T11576 cc and,cells
R7144 T11579 T11580 amod embryoid,bodies
R7145 T11580 T11576 conj bodies,cells
R7146 T11581 T11570 punct .,recombination
R7147 T11583 T11584 compound ES,cell
R7148 T11584 T11585 compound cell,clones
R7149 T11585 T11586 nsubjpass clones,transfected
R715 T1217 T1218 npadvmod chromatin,remodelling
R7150 T11587 T11585 acl bearing,clones
R7151 T11588 T11589 det the,allele
R7152 T11589 T11587 dobj allele,bearing
R7153 T11590 T11589 compound Atrx flox,allele
R7154 T11591 T11592 punct (,cells
R7155 T11592 T11585 parataxis cells,clones
R7156 T11593 T11594 quantmod 1,107
R7157 T11594 T11592 nummod 107,cells
R7158 T11595 T11594 punct ×,107
R7159 T11596 T11592 punct ),cells
R716 T1218 T1213 amod remodelling,protein
R7160 T11597 T11586 auxpass were,transfected
R7161 T11598 T11586 advmod transiently,transfected
R7162 T11599 T11586 prep with,transfected
R7163 T11600 T11601 nummod 50,μg
R7164 T11601 T11599 pobj μg,with
R7165 T11602 T11601 prep of,μg
R7166 T11603 T11604 amod uncut,plasmid
R7167 T11604 T11602 pobj plasmid,of
R7168 T11605 T11606 compound Cre,expression
R7169 T11606 T11604 compound expression,plasmid
R717 T1219 T1218 punct -,remodelling
R7170 T11607 T11604 punct (,plasmid
R7171 T11608 T11609 compound pCAGGS,IRESpuro
R7172 T11609 T11604 appos IRESpuro,plasmid
R7173 T11610 T11609 punct -,IRESpuro
R7174 T11611 T11609 compound Cre,IRESpuro
R7175 T11612 T11609 punct -,IRESpuro
R7176 T11613 T11586 punct ),transfected
R7177 T11614 T11615 punct [,40
R7178 T11615 T11586 parataxis 40,transfected
R7179 T11616 T11615 punct ],40
R718 T1220 T1209 punct ", ",regulates
R7180 T11617 T11586 punct .,transfected
R7181 T11619 T11620 prep Following,plated
R7182 T11621 T11619 pobj transfection,Following
R7183 T11622 T11620 punct ", ",plated
R7184 T11623 T11620 nsubjpass cells,plated
R7185 T11624 T11620 auxpass were,plated
R7186 T11625 T11620 prep at,plated
R7187 T11626 T11627 det a,range
R7188 T11627 T11625 pobj range,at
R7189 T11628 T11627 prep of,range
R719 T1221 T1209 nsubj ATRX,regulates
R7190 T11629 T11630 amod clonal,densities
R7191 T11630 T11628 pobj densities,of
R7192 T11631 T11620 prep in,plated
R7193 T11632 T11633 amod complete,medium
R7194 T11633 T11631 pobj medium,in
R7195 T11634 T11633 prep without,medium
R7196 T11635 T11634 pobj G418,without
R7197 T11636 T11620 punct ", ",plated
R7198 T11637 T11620 cc and,plated
R7199 T11638 T11639 amod isolated,subclones
R720 T1222 T1209 advmod probably,regulates
R7200 T11639 T11640 nsubjpass subclones,picked
R7201 T11640 T11620 conj picked,plated
R7202 T11641 T11640 auxpass were,picked
R7203 T11642 T11640 prep after,picked
R7204 T11643 T11644 nummod 7,d
R7205 T11644 T11642 pobj d,after
R7206 T11645 T11620 punct .,plated
R7207 T11647 T11648 nsubjpass Subclones,expanded
R7208 T11649 T11648 auxpass were,expanded
R7209 T11650 T11648 cc and,expanded
R721 T1223 T1209 dobj transcription,regulates
R7210 T11651 T11648 conj analysed,expanded
R7211 T11652 T11651 prep for,analysed
R7212 T11653 T11654 det the,presence
R7213 T11654 T11652 pobj presence,for
R7214 T11655 T11654 prep of,presence
R7215 T11656 T11657 det a,locus
R7216 T11657 T11655 pobj locus,of
R7217 T11658 T11657 amod recombinant,locus
R7218 T11659 T11660 advmod initially,by
R7219 T11660 T11651 prep by,analysed
R722 T1224 T1223 prep of,transcription
R7220 T11661 T11660 pobj PCR,by
R7221 T11662 T11660 punct ", ",by
R7222 T11663 T11664 aux to,detect
R7223 T11664 T11660 advcl detect,by
R7224 T11665 T11664 dobj deletion,detect
R7225 T11666 T11665 prep of,deletion
R7226 T11667 T11668 det the,cassette
R7227 T11668 T11666 pobj cassette,of
R7228 T11669 T11668 compound MC1neopA,cassette
R7229 T11670 T11660 punct ", ",by
R723 T1225 T1226 det a,set
R7230 T11671 T11660 cc and,by
R7231 T11672 T11673 advmod then,by
R7232 T11673 T11660 conj by,by
R7233 T11674 T11675 compound Southern,blot
R7234 T11675 T11673 pobj blot,by
R7235 T11676 T11675 cc and,blot
R7236 T11677 T11675 conj hybridisation,blot
R7237 T11678 T11675 prep with,blot
R7238 T11679 T11680 det the,probe
R7239 T11680 T11678 pobj probe,with
R724 T1226 T1224 pobj set,of
R7240 T11681 T11680 nmod intron,probe
R7241 T11682 T11681 nummod 17,intron
R7242 T11683 T11680 acl described,probe
R7243 T11684 T11683 advmod above,described
R7244 T11685 T11686 punct (,2A
R7245 T11686 T11651 parataxis 2A,analysed
R7246 T11687 T11686 compound Figure,2A
R7247 T11688 T11686 cc and,2A
R7248 T11689 T11686 conj 2C,2A
R7249 T11690 T11686 punct ),2A
R725 T1227 T1226 amod discrete,set
R7250 T11691 T11648 punct .,expanded
R7251 T11693 T11694 compound Northern,blots
R7252 T11694 T11695 nsubjpass blots,carried
R7253 T11696 T11695 auxpass were,carried
R7254 T11697 T11695 prt out,carried
R7255 T11698 T11695 prep according,carried
R7256 T11699 T11698 prep to,according
R7257 T11700 T11701 amod standard,techniques
R7258 T11701 T11699 pobj techniques,to
R7259 T11702 T11695 advcl using,carried
R726 T1228 T1226 prep of,set
R7260 T11703 T11704 nummod 20,μg
R7261 T11704 T11702 dobj μg,using
R7262 T11705 T11704 prep of,μg
R7263 T11706 T11707 amod total,RNA
R7264 T11707 T11705 pobj RNA,of
R7265 T11708 T11707 acl isolated,RNA
R7266 T11709 T11708 advcl using,isolated
R7267 T11710 T11711 compound TRI,Reagent
R7268 T11711 T11709 dobj Reagent,using
R7269 T11712 T11713 punct (,Aldrich
R727 T1229 T1230 compound target,genes
R7270 T11713 T11711 parataxis Aldrich,Reagent
R7271 T11714 T11713 compound Sigma,Aldrich
R7272 T11715 T11713 punct -,Aldrich
R7273 T11716 T11713 punct ", ",Aldrich
R7274 T11717 T11718 compound St.,Louis
R7275 T11718 T11713 npadvmod Louis,Aldrich
R7276 T11719 T11713 punct ", ",Aldrich
R7277 T11720 T11713 npadvmod Missouri,Aldrich
R7278 T11721 T11713 punct ", ",Aldrich
R7279 T11722 T11723 compound United,States
R728 T1230 T1228 pobj genes,of
R7280 T11723 T11713 npadvmod States,Aldrich
R7281 T11724 T11713 punct ),Aldrich
R7282 T11725 T11695 punct .,carried
R7283 T11727 T11728 det The,blot
R7284 T11728 T11729 nsubjpass blot,hybridised
R7285 T11730 T11729 auxpass was,hybridised
R7286 T11731 T11729 prep with,hybridised
R7287 T11732 T11733 det a,probe
R7288 T11733 T11731 pobj probe,with
R7289 T11734 T11733 prep from,probe
R729 T1231 T1196 punct .,suggests
R7290 T11735 T11734 prep within,from
R7291 T11736 T11735 pobj exon,within
R7292 T11737 T11736 nummod 10,exon
R7293 T11738 T11736 prep of,exon
R7294 T11739 T11740 det the,gene
R7295 T11740 T11738 pobj gene,of
R7296 T11741 T11740 compound Atrx,gene
R7297 T11742 T11733 punct (,probe
R7298 T11743 T11733 acl generated,probe
R7299 T11744 T11743 prep with,generated
R730 T1233 T1234 advmod However,remain
R7300 T11745 T11746 compound primers,Mxnp4
R7301 T11746 T11744 pobj Mxnp4,with
R7302 T11747 T11746 cc and,Mxnp4
R7303 T11748 T11746 conj Mxnp28,Mxnp4
R7304 T11749 T11750 punct [,S1
R7305 T11750 T11729 parataxis S1,hybridised
R7306 T11751 T11750 compound Table,S1
R7307 T11752 T11750 punct ],S1
R7308 T11753 T11729 punct ),hybridised
R7309 T11754 T11729 punct .,hybridised
R731 T1235 T1234 punct ", ",remain
R7310 T11756 T11757 mark After,stripped
R7311 T11757 T11760 advcl stripped,hybridised
R7312 T11758 T11757 nsubjpass it,stripped
R7313 T11759 T11757 auxpass was,stripped
R7314 T11761 T11760 punct ", ",hybridised
R7315 T11762 T11763 det the,membrane
R7316 T11763 T11760 nsubjpass membrane,hybridised
R7317 T11764 T11760 auxpass was,hybridised
R7318 T11765 T11760 prep with,hybridised
R7319 T11766 T11767 det a,probe
R732 T1236 T1237 mark although,are
R7320 T11767 T11765 pobj probe,with
R7321 T11768 T11769 compound β,actin
R7322 T11769 T11767 compound actin,probe
R7323 T11770 T11769 punct -,actin
R7324 T11771 T11767 compound cDNA,probe
R7325 T11772 T11773 punct (,Clontech
R7326 T11773 T11767 parataxis Clontech,probe
R7327 T11774 T11773 punct ", ",Clontech
R7328 T11775 T11776 compound Palo,Alto
R7329 T11776 T11773 npadvmod Alto,Clontech
R733 T1237 T1234 advcl are,remain
R7330 T11777 T11773 punct ", ",Clontech
R7331 T11778 T11773 npadvmod California,Clontech
R7332 T11779 T11773 punct ", ",Clontech
R7333 T11780 T11781 compound United,States
R7334 T11781 T11773 npadvmod States,Clontech
R7335 T11782 T11773 punct ),Clontech
R7336 T11783 T11760 punct .,hybridised
R7337 T11785 T11786 compound Protein,extraction
R7338 T11786 T11787 nsubjpass extraction,performed
R7339 T11788 T11786 cc and,extraction
R734 T1238 T1237 expl there,are
R7340 T11789 T11786 conj detection,extraction
R7341 T11790 T11789 prep of,detection
R7342 T11791 T11790 pobj Atrx,of
R7343 T11792 T11789 prep by,detection
R7344 T11793 T11794 compound Western,blotting
R7345 T11794 T11792 pobj blotting,by
R7346 T11795 T11787 auxpass was,performed
R7347 T11796 T11797 mark as,described
R7348 T11797 T11787 advcl described,performed
R7349 T11798 T11797 advmod previously,described
R735 T1239 T1237 advmod clearly,are
R7350 T11799 T11800 punct [,4
R7351 T11800 T11797 parataxis 4,described
R7352 T11801 T11800 punct ],4
R7353 T11802 T11787 punct ", ",performed
R7354 T11803 T11787 advcl using,performed
R7355 T11804 T11805 det the,antibody
R7356 T11805 T11803 dobj antibody,using
R7357 T11806 T11805 nmod mouse,antibody
R7358 T11807 T11805 amod monoclonal,antibody
R7359 T11808 T11805 amod anti-ATRX,antibody
R736 T1240 T1237 attr others,are
R7360 T11809 T11805 appos 23C,antibody
R7361 T11810 T11811 punct [,15
R7362 T11811 T11787 parataxis 15,performed
R7363 T11812 T11811 punct ],15
R7364 T11813 T11787 punct .,performed
R7365 T11815 T11816 nsubjpass Analyses,described
R7366 T11817 T11815 prep of,Analyses
R7367 T11818 T11819 compound cell,cycle
R7368 T11819 T11817 pobj cycle,of
R7369 T11820 T11819 cc and,cycle
R737 T1241 T1242 aux to,found
R7370 T11821 T11819 conj apoptosis,cycle
R7371 T11822 T11816 auxpass are,described
R7372 T11823 T11816 prep in,described
R7373 T11824 T11825 compound Protocol,S1
R7374 T11825 T11823 pobj S1,in
R7375 T11826 T11816 punct .,described
R7376 T11828 T11829 nsubjpass Methylation,analysed
R7377 T11830 T11828 prep of,Methylation
R7378 T11831 T11830 pobj rDNA,of
R7379 T11832 T11829 auxpass was,analysed
R738 T1242 T1240 advcl found,others
R7380 T11833 T11829 prep in,analysed
R7381 T11834 T11833 pobj DNA,in
R7382 T11835 T11834 prep from,DNA
R7383 T11836 T11837 compound ES,cell
R7384 T11837 T11838 compound cell,clones
R7385 T11838 T11835 pobj clones,from
R7386 T11839 T11835 cc or,from
R7387 T11840 T11835 conj from,from
R7388 T11841 T11842 amod embryoid,bodies
R7389 T11842 T11840 pobj bodies,from
R739 T1243 T1242 auxpass be,found
R7390 T11843 T11842 acl recovered,bodies
R7391 T11844 T11843 prep after,recovered
R7392 T11845 T11846 nummod 7,d
R7393 T11846 T11844 pobj d,after
R7394 T11847 T11846 prep of,d
R7395 T11848 T11849 advmod in,vitro
R7396 T11849 T11850 amod vitro,differentiation
R7397 T11850 T11847 pobj differentiation,of
R7398 T11851 T11852 mark as,described
R7399 T11852 T11829 advcl described,analysed
R740 T1244 T1234 punct ", ",remain
R7400 T11853 T11852 advmod previously,described
R7401 T11854 T11855 punct [,41
R7402 T11855 T11829 parataxis 41,analysed
R7403 T11856 T11855 punct ],41
R7404 T11857 T11829 punct .,analysed
R7405 T11859 T11860 amod Genomic,DNA
R7406 T11860 T11861 nsubjpass DNA,digested
R7407 T11862 T11861 auxpass was,digested
R7408 T11863 T11861 prep with,digested
R7409 T11864 T11865 npadvmod methylation,sensitive
R741 T1245 T1234 prep at,remain
R7410 T11865 T11867 amod sensitive,enzymes
R7411 T11866 T11865 punct -,sensitive
R7412 T11867 T11863 pobj enzymes,with
R7413 T11868 T11867 compound restriction,enzymes
R7414 T11869 T11870 mark as,described
R7415 T11870 T11861 advcl described,digested
R7416 T11871 T11861 cc and,digested
R7417 T11872 T11861 conj analysed,digested
R7418 T11873 T11872 prep by,analysed
R7419 T11874 T11875 compound Southern,blotting
R742 T1246 T1245 amod present,at
R7420 T11875 T11873 pobj blotting,by
R7421 T11876 T11861 punct .,digested
R7422 T11878 T11879 det The,probes
R7423 T11879 T11883 nsubjpass probes,described
R7424 T11880 T11879 nmod RIB3,probes
R7425 T11881 T11880 cc and,RIB3
R7426 T11882 T11880 conj RIB4,RIB3
R7427 T11884 T11879 punct (,probes
R7428 T11885 T11886 dep which,amplified
R7429 T11886 T11879 relcl amplified,probes
R743 T1247 T1248 det the,genes
R7430 T11887 T11886 auxpass were,amplified
R7431 T11888 T11886 prep from,amplified
R7432 T11889 T11890 amod human,DNA
R7433 T11890 T11888 pobj DNA,from
R7434 T11891 T11886 punct ", ",amplified
R7435 T11892 T11886 cc but,amplified
R7436 T11893 T11886 conj cross-react,amplified
R7437 T11894 T11893 prep with,cross-react
R7438 T11895 T11896 det the,repeat
R7439 T11896 T11894 pobj repeat,with
R744 T1248 T1234 nsubj genes,remain
R7440 T11897 T11898 compound mouse,rDNA
R7441 T11898 T11896 compound rDNA,repeat
R7442 T11899 T11879 punct ),probes
R7443 T11900 T11883 aux have,described
R7444 T11901 T11883 auxpass been,described
R7445 T11902 T11883 advmod previously,described
R7446 T11903 T11904 punct [,11
R7447 T11904 T11883 parataxis 11,described
R7448 T11905 T11904 punct ],11
R7449 T11906 T11883 punct .,described
R745 T1249 T1250 compound α,globin
R7450 T11908 T11909 compound Oligonucleotide,probes
R7451 T11909 T11910 nsubjpass probes,described
R7452 T11911 T11912 aux to,detect
R7453 T11912 T11909 advcl detect,probes
R7454 T11913 T11914 nmod Line,repeats
R7455 T11914 T11912 dobj repeats,detect
R7456 T11915 T11913 nummod 1,Line
R7457 T11916 T11913 cc and,Line
R7458 T11917 T11918 compound Sine,B1
R7459 T11918 T11913 conj B1,Line
R746 T1250 T1248 compound globin,genes
R7460 T11919 T11910 aux have,described
R7461 T11920 T11910 auxpass been,described
R7462 T11921 T11910 advmod previously,described
R7463 T11922 T11923 punct [,42
R7464 T11923 T11910 parataxis 42,described
R7465 T11924 T11923 punct ],42
R7466 T11925 T11910 punct .,described
R7467 T11927 T11928 det The,probe
R7468 T11928 T11931 nsubj probe,was
R7469 T11929 T11930 amod minor,satellite
R747 T1251 T1250 punct -,globin
R7470 T11930 T11928 compound satellite,probe
R7471 T11932 T11933 det a,oligonucleotide
R7472 T11933 T11931 attr oligonucleotide,was
R7473 T11934 T11935 nummod 27,mer
R7474 T11935 T11933 compound mer,oligonucleotide
R7475 T11936 T11935 punct -,mer
R7476 T11937 T11933 punct (,oligonucleotide
R7477 T11938 T11933 appos mCENT2,oligonucleotide
R7478 T11939 T11931 punct ),was
R7479 T11940 T11931 punct .,was
R748 T1252 T1253 det the,targets
R7480 T11942 T11943 det The,probe
R7481 T11943 T11946 nsubj probe,was
R7482 T11944 T11945 amod major,satellite
R7483 T11945 T11943 compound satellite,probe
R7484 T11947 T11948 det a,oligonucleotide
R7485 T11948 T11946 attr oligonucleotide,was
R7486 T11949 T11950 nummod 27,mer
R7487 T11950 T11948 compound mer,oligonucleotide
R7488 T11951 T11950 punct -,mer
R7489 T11952 T11948 punct (,oligonucleotide
R749 T1253 T1234 oprd targets,remain
R7490 T11953 T11948 appos DG27,oligonucleotide
R7491 T11954 T11946 punct ),was
R7492 T11955 T11946 punct .,was
R7493 T11957 T11958 det The,probe
R7494 T11958 T11960 nsubj probe,was
R7495 T11959 T11958 compound IAP,probe
R7496 T11961 T11962 det an,product
R7497 T11962 T11960 attr product,was
R7498 T11963 T11964 punct ~,400
R7499 T11964 T11965 nummod 400,bp
R750 T1254 T1253 advmod only,targets
R7500 T11965 T11966 compound bp,PCR
R7501 T11966 T11962 compound PCR,product
R7502 T11967 T11962 punct (,product
R7503 T11968 T11969 compound primers,14A
R7504 T11969 T11962 appos 14A,product
R7505 T11970 T11969 cc and,14A
R7506 T11971 T11969 conj 13K,14A
R7507 T11972 T11962 punct ),product
R7508 T11973 T11962 acl amplified,product
R7509 T11974 T11973 prep from,amplified
R751 T1255 T1253 amod confirmed,targets
R7510 T11975 T11976 det an,IAP
R7511 T11976 T11974 pobj IAP,from
R7512 T11977 T11976 acl inserted,IAP
R7513 T11978 T11977 prep into,inserted
R7514 T11979 T11980 det the,gene
R7515 T11980 T11978 pobj gene,into
R7516 T11981 T11982 compound mouse,agouti
R7517 T11982 T11980 compound agouti,gene
R7518 T11983 T11984 punct [,43
R7519 T11984 T11960 parataxis 43,was
R752 T1256 T1253 prep for,targets
R7520 T11985 T11984 punct ],43
R7521 T11986 T11960 cc and,was
R7522 T11987 T11960 conj was,was
R7523 T11988 T11989 det a,gift
R7524 T11989 T11987 attr gift,was
R7525 T11990 T11989 prep from,gift
R7526 T11991 T11992 compound Peter,Warnecke
R7527 T11992 T11990 pobj Warnecke,from
R7528 T11993 T11992 cc and,Warnecke
R7529 T11994 T11995 compound Tim,Bestor
R753 T1257 T1258 amod transcriptional,regulation
R7530 T11995 T11992 conj Bestor,Warnecke
R7531 T11996 T11960 punct .,was
R7532 T11998 T11999 det The,product
R7533 T11999 T12001 nsubj product,included
R7534 T12000 T11999 compound PCR,product
R7535 T12002 T12003 det the,LTR
R7536 T12003 T12001 dobj LTR,included
R7537 T12004 T12003 amod entire,LTR
R7538 T12005 T12003 nummod 5,LTR
R7539 T12006 T12005 punct ′,5
R754 T1258 T1256 pobj regulation,for
R7540 T12007 T12003 prep of,LTR
R7541 T12008 T12009 det the,IAP
R7542 T12009 T12007 pobj IAP,of
R7543 T12010 T12001 punct .,included
R7544 T12012 T12013 det All,sequences
R7545 T12013 T12015 nsubjpass sequences,shown
R7546 T12014 T12013 compound oligonucleotide,sequences
R7547 T12016 T12015 auxpass are,shown
R7548 T12017 T12015 prep in,shown
R7549 T12018 T12019 compound Table,S1
R755 T1259 T1258 prep by,regulation
R7550 T12019 T12017 pobj S1,in
R7551 T12020 T12015 punct .,shown
R7552 T12081 T12080 prep of,Generation
R7553 T12082 T12081 pobj chimeras,of
R7554 T12083 T12082 punct ", ",chimeras
R7555 T12084 T12085 amod floxed,mice
R7556 T12085 T12082 conj mice,chimeras
R7557 T12086 T12085 punct ", ",mice
R7558 T12087 T12085 cc and,mice
R7559 T12088 T12089 compound mutant,mice
R756 T1260 T1259 pobj ATRX,by
R7560 T12089 T12085 conj mice,mice
R7561 T12090 T12080 punct .,Generation
R7562 T12092 T12093 amod Targeted,clones
R7563 T12093 T12097 nsubjpass clones,injected
R7564 T12094 T12095 compound Atrx flox,cell
R7565 T12095 T12093 compound cell,clones
R7566 T12096 T12095 compound ES,cell
R7567 T12098 T12097 auxpass were,injected
R7568 T12099 T12097 prep into,injected
R7569 T12100 T12101 nmod C57BL,blastocysts
R757 T1261 T1234 punct .,remain
R7570 T12101 T12099 pobj blastocysts,into
R7571 T12102 T12100 punct /,C57BL
R7572 T12103 T12100 nummod 6,C57BL
R7573 T12104 T12097 cc and,injected
R7574 T12105 T12097 conj transferred,injected
R7575 T12106 T12105 prep into,transferred
R7576 T12107 T12108 nummod 2.5,dpc
R7577 T12108 T12109 nmod dpc,recipients
R7578 T12109 T12106 pobj recipients,into
R7579 T12110 T12109 amod pseudopregnant,recipients
R758 T1263 T1264 nsubjpass Little,known
R7580 T12111 T12097 prep by,injected
R7581 T12112 T12113 amod standard,techniques
R7582 T12113 T12111 pobj techniques,by
R7583 T12114 T12097 punct .,injected
R7584 T12116 T12117 amod Resulting,chimeras
R7585 T12117 T12118 nsubjpass chimeras,mated
R7586 T12119 T12118 auxpass were,mated
R7587 T12120 T12118 prep with,mated
R7588 T12121 T12120 pobj C57BL,with
R7589 T12122 T12121 punct /,C57BL
R759 T1265 T1264 auxpass is,known
R7590 T12123 T12121 nummod 6,C57BL
R7591 T12124 T12125 aux to,establish
R7592 T12125 T12118 advcl establish,mated
R7593 T12126 T12127 compound germline,transmission
R7594 T12127 T12125 dobj transmission,establish
R7595 T12128 T12118 punct .,mated
R7596 T12130 T12131 nsubjpass Offspring,identified
R7597 T12132 T12130 prep with,Offspring
R7598 T12133 T12134 det the,allele
R7599 T12134 T12132 pobj allele,with
R760 T1266 T1264 advmod currently,known
R7600 T12135 T12134 compound Atrx flox,allele
R7601 T12136 T12131 auxpass were,identified
R7602 T12137 T12131 prep by,identified
R7603 T12138 T12139 compound Southern,blotting
R7604 T12139 T12137 pobj blotting,by
R7605 T12140 T12139 prep of,blotting
R7606 T12141 T12142 npadvmod SacI,digested
R7607 T12142 T12144 amod digested,DNA
R7608 T12143 T12142 punct -,digested
R7609 T12144 T12140 pobj DNA,of
R761 T1267 T1264 prep about,known
R7610 T12145 T12144 compound tail,DNA
R7611 T12146 T12131 advcl using,identified
R7612 T12147 T12148 det the,probe
R7613 T12148 T12146 dobj probe,using
R7614 T12149 T12148 nmod intron,probe
R7615 T12150 T12149 nummod 17,intron
R7616 T12151 T12152 mark as,shown
R7617 T12152 T12146 advcl shown,using
R7618 T12153 T12152 prep in,shown
R7619 T12154 T12155 compound Figure,2A
R762 T1268 T1269 det the,role
R7620 T12155 T12153 pobj 2A,in
R7621 T12156 T12155 cc and,2A
R7622 T12157 T12155 conj 2C,2A
R7623 T12158 T12131 punct .,identified
R7624 T12160 T12161 prep For,crossed
R7625 T12162 T12163 compound Cre,recombination
R7626 T12163 T12160 pobj recombination,For
R7627 T12164 T12163 punct -,recombination
R7628 T12165 T12161 punct ", ",crossed
R7629 T12166 T12167 compound Atrx flox,mice
R763 T1269 T1267 pobj role,about
R7630 T12167 T12161 nsubjpass mice,crossed
R7631 T12168 T12161 auxpass were,crossed
R7632 T12169 T12161 prep with,crossed
R7633 T12170 T12171 compound GATA1,Cre
R7634 T12171 T12173 npadvmod Cre,transgenic
R7635 T12172 T12171 punct -,Cre
R7636 T12173 T12174 amod transgenic,mice
R7637 T12174 T12169 pobj mice,with
R7638 T12175 T12176 mark as,described
R7639 T12176 T12161 advcl described,crossed
R764 T1270 T1269 amod precise,role
R7640 T12177 T12176 prep in,described
R7641 T12178 T12179 det the,text
R7642 T12179 T12177 pobj text,in
R7643 T12180 T12161 punct .,crossed
R7644 T12182 T12183 amod Recombinant,alleles
R7645 T12183 T12184 nsubjpass alleles,detected
R7646 T12185 T12184 auxpass were,detected
R7647 T12186 T12184 prep by,detected
R7648 T12187 T12188 compound Southern,blotting
R7649 T12188 T12186 pobj blotting,by
R765 T1271 T1269 prep of,role
R7650 T12189 T12190 mark as,described
R7651 T12190 T12188 advcl described,blotting
R7652 T12191 T12190 advmod above,described
R7653 T12192 T12186 cc or,by
R7654 T12193 T12186 conj by,by
R7655 T12194 T12193 pobj PCR,by
R7656 T12195 T12196 mark as,described
R7657 T12196 T12184 advcl described,detected
R7658 T12197 T12196 prep in,described
R7659 T12198 T12199 compound Protocol,S1
R766 T1272 T1273 det the,protein
R7660 T12199 T12197 pobj S1,in
R7661 T12200 T12184 punct .,detected
R7662 T12293 T12292 punct ", ",Immunohistochemistry
R7663 T12294 T12295 nmod in,situ
R7664 T12295 T12296 nmod situ,hybridisation
R7665 T12296 T12292 conj hybridisation,Immunohistochemistry
R7666 T12297 T12296 punct ", ",hybridisation
R7667 T12298 T12296 cc and,hybridisation
R7668 T12299 T12300 compound TUNEL,assay
R7669 T12300 T12296 conj assay,hybridisation
R767 T1273 T1271 pobj protein,of
R7670 T12301 T12300 punct .,assay
R7671 T12303 T12304 nummod 7.5,dpc
R7672 T12304 T12305 nmod dpc,swellings
R7673 T12305 T12307 nsubjpass swellings,dissected
R7674 T12306 T12305 amod decidual,swellings
R7675 T12308 T12307 auxpass were,dissected
R7676 T12309 T12307 advmod away,dissected
R7677 T12310 T12309 prep from,away
R7678 T12311 T12312 amod maternal,tissue
R7679 T12312 T12310 pobj tissue,from
R768 T1274 T1273 compound ATRX,protein
R7680 T12313 T12307 cc and,dissected
R7681 T12314 T12307 conj fixed,dissected
R7682 T12315 T12314 prep in,fixed
R7683 T12316 T12317 nummod 4,%
R7684 T12317 T12318 compound %,paraformaldehyde
R7685 T12318 T12315 pobj paraformaldehyde,in
R7686 T12319 T12318 punct /,paraformaldehyde
R7687 T12320 T12318 appos PBS,paraformaldehyde
R7688 T12321 T12314 advmod overnight,fixed
R7689 T12322 T12314 prep at,fixed
R769 T1275 T1269 prep during,role
R7690 T12323 T12324 nummod 4,°C
R7691 T12324 T12322 pobj °C,at
R7692 T12325 T12307 punct .,dissected
R7693 T12327 T12328 mark After,washed
R7694 T12328 T12331 advcl washed,dehydrated
R7695 T12329 T12328 nsubjpass embryos,washed
R7696 T12330 T12328 auxpass were,washed
R7697 T12332 T12328 advmod thoroughly,washed
R7698 T12333 T12328 prep in,washed
R7699 T12334 T12333 pobj PBS,in
R770 T1276 T1277 amod mammalian,development
R7700 T12335 T12331 punct ", ",dehydrated
R7701 T12336 T12331 nsubjpass they,dehydrated
R7702 T12337 T12331 auxpass were,dehydrated
R7703 T12338 T12331 prep through,dehydrated
R7704 T12339 T12340 det an,series
R7705 T12340 T12338 pobj series,through
R7706 T12341 T12340 compound ethanol,series
R7707 T12342 T12340 cc and,series
R7708 T12343 T12340 conj xylene,series
R7709 T12344 T12331 punct ", ",dehydrated
R771 T1277 T1275 pobj development,during
R7710 T12345 T12331 conj embedded,dehydrated
R7711 T12346 T12345 prep in,embedded
R7712 T12347 T12346 pobj paraffin,in
R7713 T12348 T12345 punct ", ",embedded
R7714 T12349 T12345 cc and,embedded
R7715 T12350 T12345 conj sectioned,embedded
R7716 T12351 T12350 prep at,sectioned
R7717 T12352 T12353 nummod 5,μm
R7718 T12353 T12351 pobj μm,at
R7719 T12354 T12331 punct .,dehydrated
R772 T1278 T1264 punct .,known
R7720 T12356 T12357 nsubjpass Sections,processed
R7721 T12358 T12357 auxpass were,processed
R7722 T12359 T12357 prep for,processed
R7723 T12360 T12359 pobj immunohistochemistry,for
R7724 T12361 T12357 advcl using,processed
R7725 T12362 T12363 det the,System
R7726 T12363 T12361 dobj System,using
R7727 T12364 T12363 compound ABC,System
R7728 T12365 T12363 compound Staining,System
R7729 T12366 T12367 punct (,Biotechnology
R773 T1280 T1281 aux To,investigate
R7730 T12367 T12363 parataxis Biotechnology,System
R7731 T12368 T12369 compound Santa,Cruz
R7732 T12369 T12367 compound Cruz,Biotechnology
R7733 T12370 T12367 punct ", ",Biotechnology
R7734 T12371 T12372 compound Santa,Cruz
R7735 T12372 T12367 npadvmod Cruz,Biotechnology
R7736 T12373 T12367 punct ", ",Biotechnology
R7737 T12374 T12367 npadvmod California,Biotechnology
R7738 T12375 T12367 punct ", ",Biotechnology
R7739 T12376 T12377 compound United,States
R774 T1281 T1282 advcl investigate,generated
R7740 T12377 T12367 npadvmod States,Biotechnology
R7741 T12378 T12367 punct ),Biotechnology
R7742 T12379 T12361 prep according,using
R7743 T12380 T12379 prep to,according
R7744 T12381 T12382 det the,manufacturer
R7745 T12382 T12383 poss manufacturer,instructions
R7746 T12383 T12380 pobj instructions,to
R7747 T12384 T12382 case 's,manufacturer
R7748 T12385 T12357 punct .,processed
R7749 T12387 T12388 nsubjpass Sections,stained
R775 T1283 T1284 det the,role
R7750 T12389 T12388 auxpass were,stained
R7751 T12390 T12388 prep with,stained
R7752 T12391 T12392 nmod rabbit,antibodies
R7753 T12392 T12390 pobj antibodies,with
R7754 T12393 T12392 amod polyclonal,antibodies
R7755 T12394 T12392 prep against,antibodies
R7756 T12395 T12394 pobj ATRX,against
R7757 T12396 T12397 punct (,H
R7758 T12397 T12395 parataxis H,ATRX
R7759 T12398 T12397 punct -,H
R776 T1284 T1281 dobj role,investigate
R7760 T12399 T12397 nummod 300,H
R7761 T12400 T12397 punct ", ",H
R7762 T12401 T12402 compound Santa,Cruz
R7763 T12402 T12403 compound Cruz,Biotechnology
R7764 T12403 T12397 npadvmod Biotechnology,H
R7765 T12404 T12397 punct ),H
R7766 T12405 T12395 punct ", ",ATRX
R7767 T12406 T12407 amod Placental,I
R7768 T12407 T12395 conj I,ATRX
R7769 T12408 T12407 compound lactogen,I
R777 T1285 T1284 prep of,role
R7770 T12409 T12407 punct -,I
R7771 T12410 T12411 punct (,AB1288
R7772 T12411 T12407 parataxis AB1288,I
R7773 T12412 T12411 punct ", ",AB1288
R7774 T12413 T12414 compound Chemicon,International
R7775 T12414 T12411 npadvmod International,AB1288
R7776 T12415 T12411 punct ", ",AB1288
R7777 T12416 T12411 npadvmod Temecula,AB1288
R7778 T12417 T12411 punct ", ",AB1288
R7779 T12418 T12411 npadvmod California,AB1288
R778 T1286 T1287 det this,protein
R7780 T12419 T12411 punct ", ",AB1288
R7781 T12420 T12421 compound United,States
R7782 T12421 T12411 npadvmod States,AB1288
R7783 T12422 T12411 punct ),AB1288
R7784 T12423 T12407 cc and,I
R7785 T12424 T12425 nmod phospho,histone
R7786 T12425 T12430 compound histone,H3
R7787 T12426 T12427 punct (,Ser10
R7788 T12427 T12425 parataxis Ser10,histone
R7789 T12428 T12427 punct ),Ser10
R779 T1287 T1285 pobj protein,of
R7790 T12429 T12425 punct -,histone
R7791 T12430 T12407 conj H3,I
R7792 T12431 T12432 punct (,570
R7793 T12432 T12430 parataxis 570,H3
R7794 T12433 T12432 nummod 06,570
R7795 T12434 T12432 punct –,570
R7796 T12435 T12432 punct ", ",570
R7797 T12436 T12437 compound Upstate,Biotechnology
R7798 T12437 T12432 npadvmod Biotechnology,570
R7799 T12438 T12432 punct ", ",570
R780 T1288 T1284 prep during,role
R7800 T12439 T12432 npadvmod Waltham,570
R7801 T12440 T12432 punct ", ",570
R7802 T12441 T12432 npadvmod Massachusetts,570
R7803 T12442 T12432 punct ", ",570
R7804 T12443 T12444 compound United,States
R7805 T12444 T12432 npadvmod States,570
R7806 T12445 T12432 punct ),570
R7807 T12446 T12388 punct .,stained
R7808 T12448 T12449 advmod Where,appropriate
R7809 T12449 T12450 advcl appropriate,stained
R781 T1289 T1290 compound mouse,development
R7810 T12451 T12450 punct ", ",stained
R7811 T12452 T12453 amod adjacent,sections
R7812 T12453 T12450 nsubjpass sections,stained
R7813 T12454 T12450 auxpass were,stained
R7814 T12455 T12450 prep with,stained
R7815 T12456 T12455 pobj haematoxylin,with
R7816 T12457 T12450 punct .,stained
R7817 T12459 T12460 prep In,analysed
R7818 T12461 T12462 det some,cases
R7819 T12462 T12459 pobj cases,In
R782 T1290 T1288 pobj development,during
R7820 T12463 T12460 punct ", ",analysed
R7821 T12464 T12465 amod adjacent,sections
R7822 T12465 T12460 nsubjpass sections,analysed
R7823 T12466 T12460 auxpass were,analysed
R7824 T12467 T12460 advmod also,analysed
R7825 T12468 T12469 aux to,detect
R7826 T12469 T12460 advcl detect,analysed
R7827 T12470 T12471 amod apoptotic,cells
R7828 T12471 T12469 dobj cells,detect
R7829 T12472 T12469 prep by,detect
R783 T1291 T1282 punct ", ",generated
R7830 T12473 T12472 pobj TUNEL,by
R7831 T12474 T12469 advcl using,detect
R7832 T12475 T12476 det the,kit
R7833 T12476 T12474 dobj kit,using
R7834 T12477 T12478 advmod in,situ
R7835 T12478 T12476 amod situ,kit
R7836 T12479 T12480 compound cell,death
R7837 T12480 T12481 compound death,detection
R7838 T12481 T12476 compound detection,kit
R7839 T12482 T12483 punct (,Roche
R784 T1292 T1282 nsubj we,generated
R7840 T12483 T12474 parataxis Roche,using
R7841 T12484 T12483 punct ", ",Roche
R7842 T12485 T12483 npadvmod Basel,Roche
R7843 T12486 T12483 punct ", ",Roche
R7844 T12487 T12483 npadvmod Switzerland,Roche
R7845 T12488 T12483 punct ),Roche
R7846 T12489 T12460 punct .,analysed
R7847 T12491 T12492 prep After,mounted
R7848 T12493 T12491 pobj labelling,After
R7849 T12494 T12492 punct ", ",mounted
R785 T1293 T1294 det a,allele
R7850 T12495 T12496 det these,slides
R7851 T12496 T12492 nsubjpass slides,mounted
R7852 T12497 T12492 auxpass were,mounted
R7853 T12498 T12492 prep in,mounted
R7854 T12499 T12498 pobj Vectashield,in
R7855 T12500 T12499 acl containing,Vectashield
R7856 T12501 T12500 dobj DAPI,containing
R7857 T12502 T12503 punct (,Laboratories
R7858 T12503 T12499 parataxis Laboratories,Vectashield
R7859 T12504 T12503 compound Vector,Laboratories
R786 T1294 T1282 dobj allele,generated
R7860 T12505 T12503 punct ", ",Laboratories
R7861 T12506 T12503 npadvmod Burlingame,Laboratories
R7862 T12507 T12503 punct ", ",Laboratories
R7863 T12508 T12503 npadvmod California,Laboratories
R7864 T12509 T12503 punct ", ",Laboratories
R7865 T12510 T12511 compound United,States
R7866 T12511 T12503 npadvmod States,Laboratories
R7867 T12512 T12503 punct ),Laboratories
R7868 T12513 T12492 cc and,mounted
R7869 T12514 T12492 conj visualised,mounted
R787 T1295 T1296 advmod conditionally,deleted
R7870 T12515 T12514 prep by,visualised
R7871 T12516 T12517 compound fluorescence,microscopy
R7872 T12517 T12515 pobj microscopy,by
R7873 T12518 T12492 punct .,mounted
R7874 T12520 T12521 amod Whole,mount
R7875 T12521 T12523 nmod mount,hybridisations
R7876 T12522 T12521 punct -,mount
R7877 T12523 T12526 nsubjpass hybridisations,performed
R7878 T12524 T12525 advmod in,situ
R7879 T12525 T12523 amod situ,hybridisations
R788 T1296 T1294 amod deleted,allele
R7880 T12527 T12526 auxpass were,performed
R7881 T12528 T12526 prep on,performed
R7882 T12529 T12530 nummod 8.5,dpc
R7883 T12530 T12531 compound dpc,embryos
R7884 T12531 T12528 pobj embryos,on
R7885 T12532 T12531 punct (,embryos
R7886 T12533 T12531 acl dissected,embryos
R7887 T12534 T12533 advmod away,dissected
R7888 T12535 T12534 prep from,away
R7889 T12536 T12537 amod maternal,tissues
R789 T1297 T1294 prep of,allele
R7890 T12537 T12535 pobj tissues,from
R7891 T12538 T12536 cc and,maternal
R7892 T12539 T12536 conj extraembryonic,maternal
R7893 T12540 T12533 punct ),dissected
R7894 T12541 T12533 advcl using,dissected
R7895 T12542 T12543 det a,riboprobe
R7896 T12543 T12541 dobj riboprobe,using
R7897 T12544 T12543 nmod brachyury,riboprobe
R7898 T12545 T12544 punct (,brachyury
R7899 T12546 T12544 appos T,brachyury
R790 T1298 T1299 det the,gene
R7900 T12547 T12543 punct ),riboprobe
R7901 T12548 T12549 punct [,16
R7902 T12549 T12541 parataxis 16,using
R7903 T12550 T12549 punct ],16
R7904 T12551 T12528 cc and,on
R7905 T12552 T12528 conj on,on
R7906 T12553 T12554 amod bisected,sites
R7907 T12554 T12552 pobj sites,on
R7908 T12555 T12554 amod decidual,sites
R7909 T12556 T12554 compound implantation,sites
R791 T1299 T1297 pobj gene,of
R7910 T12557 T12558 prep from,removed
R7911 T12558 T12554 relcl removed,sites
R7912 T12559 T12557 pobj which,from
R7913 T12560 T12558 nsubjpass embryos,removed
R7914 T12561 T12562 punct (,dpc
R7915 T12562 T12560 parataxis dpc,embryos
R7916 T12563 T12562 nummod 8.5,dpc
R7917 T12564 T12562 punct ),dpc
R7918 T12565 T12558 aux had,removed
R7919 T12566 T12558 auxpass been,removed
R792 T1300 T1299 compound Atrx,gene
R7920 T12567 T12558 advcl using,removed
R7921 T12568 T12569 det a,riboprobe
R7922 T12569 T12567 dobj riboprobe,using
R7923 T12570 T12571 amod placental,lactogen
R7924 T12571 T12569 nmod lactogen,riboprobe
R7925 T12572 T12571 punct -,lactogen
R7926 T12573 T12571 nummod 1,lactogen
R7927 T12574 T12571 punct (,lactogen
R7928 T12575 T12571 appos Pl,lactogen
R7929 T12576 T12575 punct -,Pl
R793 T1301 T1282 prep in,generated
R7930 T12577 T12575 nummod 1,Pl
R7931 T12578 T12569 punct ),riboprobe
R7932 T12579 T12580 punct (,see
R7933 T12580 T12558 parataxis see,removed
R7934 T12581 T12582 compound Protocol,S1
R7935 T12582 T12580 dobj S1,see
R7936 T12583 T12580 prep for,see
R7937 T12584 T12583 pobj details,for
R7938 T12585 T12580 punct ),see
R7939 T12586 T12526 punct .,performed
R794 T1302 T1303 nmod mouse,cells
R7940 T12623 T12624 compound Blastocyst,outgrowth
R7941 T12624 T12625 compound outgrowth,cultures
R7942 T12626 T12625 punct .,cultures
R7943 T12628 T12629 amod Superovulated,mice
R7944 T12629 T12632 nsubjpass mice,mated
R7945 T12630 T12629 amod heterozygous,mice
R7946 T12631 T12629 amod female,mice
R7947 T12633 T12634 punct (,flox
R7948 T12634 T12629 parataxis flox,mice
R7949 T12635 T12634 compound Atrx WT,flox
R795 T1303 T1301 pobj cells,in
R7950 T12636 T12634 punct /,flox
R7951 T12637 T12634 punct ),flox
R7952 T12638 T12632 auxpass were,mated
R7953 T12639 T12632 prep to,mated
R7954 T12640 T12641 amod homozygous,males
R7955 T12641 T12639 pobj males,to
R7956 T12642 T12643 nmod GATA1,cre
R7957 T12643 T12641 nmod cre,males
R7958 T12644 T12643 punct -,cre
R7959 T12645 T12643 punct +,cre
R796 T1304 T1305 amod embryonic,stem
R7960 T12646 T12643 punct /,cre
R7961 T12647 T12643 punct +,cre
R7962 T12648 T12641 amod transgenic,males
R7963 T12649 T12632 punct ", ",mated
R7964 T12650 T12632 cc and,mated
R7965 T12651 T12652 nsubjpass blastocysts,flushed
R7966 T12652 T12632 conj flushed,mated
R7967 T12653 T12652 auxpass were,flushed
R7968 T12654 T12652 prep from,flushed
R7969 T12655 T12656 amod uterine,horns
R797 T1305 T1303 nmod stem,cells
R7970 T12656 T12654 pobj horns,from
R7971 T12657 T12652 prep with,flushed
R7972 T12658 T12659 compound M2,medium
R7973 T12659 T12657 pobj medium,with
R7974 T12660 T12661 punct (,Sigma
R7975 T12661 T12659 parataxis Sigma,medium
R7976 T12662 T12661 punct ),Sigma
R7977 T12663 T12652 prep at,flushed
R7978 T12664 T12665 nummod 3.5,dpc
R7979 T12665 T12663 pobj dpc,at
R798 T1306 T1305 punct (,stem
R7980 T12666 T12652 punct .,flushed
R7981 T12668 T12669 amod Individual,blastocysts
R7982 T12669 T12670 nsubjpass blastocysts,cultured
R7983 T12671 T12670 auxpass were,cultured
R7984 T12672 T12670 prep in,cultured
R7985 T12673 T12674 amod multiwell,cultures
R7986 T12674 T12676 compound cultures,plates
R7987 T12675 T12674 compound tissue,cultures
R7988 T12676 T12672 pobj plates,in
R7989 T12677 T12678 mark as,described
R799 T1307 T1305 appos ES,stem
R7990 T12678 T12670 advcl described,cultured
R7991 T12679 T12678 advmod previously,described
R7992 T12680 T12681 punct [,44
R7993 T12681 T12670 parataxis 44,cultured
R7994 T12682 T12681 punct ],44
R7995 T12683 T12670 punct .,cultured
R7996 T12685 T12686 nsubj Cultures,inspected
R7997 T12687 T12686 aux were,inspected
R7998 T12688 T12686 cc and,inspected
R7999 T12689 T12686 conj photographed,inspected
R800 T1308 T1303 punct ),cells
R8000 T12690 T12686 advmod daily,inspected
R8001 T12691 T12686 cc and,inspected
R8002 T12692 T12693 det the,extent
R8003 T12693 T12694 nsubj extent,scored
R8004 T12694 T12686 conj scored,inspected
R8005 T12695 T12693 prep of,extent
R8006 T12696 T12695 pobj outgrowth,of
R8007 T12697 T12694 punct .,scored
R8008 T12699 T12700 prep After,harvested
R8009 T12701 T12702 nummod 7,d
R801 T1309 T1282 punct ", ",generated
R8010 T12702 T12699 pobj d,After
R8011 T12703 T12700 punct ", ",harvested
R8012 T12704 T12700 nsubjpass cultures,harvested
R8013 T12705 T12700 auxpass were,harvested
R8014 T12706 T12700 cc and,harvested
R8015 T12707 T12708 nsubj DNA,extracted
R8016 T12708 T12700 conj extracted,harvested
R8017 T12709 T12700 punct .,harvested
R8018 T12711 T12712 det The,genotype
R8019 T12712 T12714 nsubjpass genotype,determined
R802 T1310 T1282 cc and,generated
R8020 T12713 T12712 compound Atrx,genotype
R8021 T12715 T12712 cc and,genotype
R8022 T12716 T12712 conj sex,genotype
R8023 T12717 T12712 prep of,genotype
R8024 T12718 T12719 det each,culture
R8025 T12719 T12717 pobj culture,of
R8026 T12720 T12714 auxpass was,determined
R8027 T12721 T12714 prep by,determined
R8028 T12722 T12721 pobj PCR,by
R8029 T12723 T12724 mark as,described
R803 T1311 T1282 conj used,generated
R8030 T12724 T12714 advcl described,determined
R8031 T12725 T12724 prep in,described
R8032 T12726 T12727 compound Protocol,S1
R8033 T12727 T12725 pobj S1,in
R8034 T12728 T12714 punct .,determined
R804 T1312 T1313 det these,cells
R805 T1313 T1311 dobj cells,used
R806 T1314 T1315 aux to,examine
R807 T1315 T1311 advcl examine,used
R808 T1316 T1317 det the,effect
R809 T1317 T1315 dobj effect,examine
R810 T1318 T1317 prep of,effect
R811 T1319 T1320 amod ablating,expression
R812 T1320 T1318 pobj expression,of
R813 T1321 T1320 prep of,expression
R814 T1322 T1323 det the,protein
R815 T1323 T1321 pobj protein,of
R816 T1324 T1325 amod full,length
R817 T1325 T1323 compound length,protein
R818 T1326 T1325 punct -,length
R819 T1327 T1323 compound Atrx,protein
R820 T1328 T1317 prep in,effect
R821 T1329 T1330 compound ES,cells
R822 T1330 T1328 pobj cells,in
R823 T1331 T1328 cc and,in
R824 T1332 T1328 conj in,in
R825 T1333 T1334 compound mouse,embryos
R826 T1334 T1332 pobj embryos,in
R827 T1335 T1282 punct .,generated
R830 T1575 T1574 prep of,Generation
R831 T1576 T1577 compound ES,Cells
R832 T1577 T1575 pobj Cells,of
R833 T1578 T1577 acl Lacking,Cells
R834 T1579 T1580 amod Full,Length
R835 T1580 T1582 compound Length,Atrx
R836 T1581 T1580 punct -,Length
R837 T1582 T1578 dobj Atrx,Lacking
R838 T1584 T1585 prep Like,is
R839 T1586 T1587 det the,gene
R840 T1587 T1584 pobj gene,Like
R841 T1588 T1587 amod human,gene
R842 T1589 T1585 punct ", ",is
R843 T1590 T1591 det the,gene
R844 T1591 T1585 nsubj gene,is
R845 T1592 T1591 compound mouse,gene
R846 T1593 T1591 compound Atrx,gene
R847 T1594 T1585 advmod also,is
R848 T1595 T1596 npadvmod X,linked
R849 T1596 T1585 acomp linked,is
R850 T1597 T1596 punct -,linked
R851 T1598 T1585 punct ", ",is
R852 T1599 T1600 amod such,give
R853 T1600 T1585 advcl give,is
R854 T1601 T1600 mark that,give
R855 T1602 T1603 det a,disruption
R856 T1603 T1600 nsubj disruption,give
R857 T1604 T1603 amod direct,disruption
R858 T1605 T1603 prep of,disruption
R859 T1606 T1607 det the,allele
R860 T1607 T1605 pobj allele,of
R861 T1608 T1607 amod single,allele
R862 T1609 T1607 compound Atrx,allele
R863 T1610 T1603 prep in,disruption
R864 T1611 T1612 amod male,cells
R865 T1612 T1610 pobj cells,in
R866 T1613 T1612 compound ES,cells
R867 T1614 T1600 aux would,give
R868 T1615 T1600 advmod immediately,give
R869 T1616 T1600 dobj rise,give
R870 T1617 T1600 prep to,give
R871 T1618 T1619 det the,state
R872 T1619 T1617 pobj state,to
R873 T1620 T1619 amod null,state
R874 T1621 T1585 punct .,is
R875 T1623 T1624 det No,clones
R876 T1624 T1626 nsubjpass clones,recovered
R877 T1625 T1624 amod targeted,clones
R878 T1627 T1626 auxpass were,recovered
R879 T1628 T1626 prep after,recovered
R880 T1629 T1630 amod attempted,recombination
R881 T1630 T1628 pobj recombination,after
R882 T1631 T1630 amod homologous,recombination
R883 T1632 T1630 prep in,recombination
R884 T1633 T1634 amod male,cells
R885 T1634 T1632 pobj cells,in
R886 T1635 T1636 compound E14TG2a,ES
R887 T1636 T1634 compound ES,cells
R888 T1637 T1626 advcl using,recovered
R889 T1638 T1639 nummod two,vectors
R890 T1639 T1637 dobj vectors,using
R891 T1640 T1639 amod different,vectors
R892 T1641 T1642 dep that,removed
R893 T1642 T1639 relcl removed,vectors
R894 T1643 T1642 dobj exon,removed
R895 T1644 T1643 nummod 18,exon
R896 T1645 T1643 prep of,exon
R897 T1646 T1647 det the,gene
R898 T1647 T1645 pobj gene,of
R899 T1648 T1647 compound Atrx,gene
R900 T1649 T1626 punct .,recovered
R901 T1651 T1652 nsubj Exon,encodes
R902 T1652 T1654 ccomp encodes,shown
R903 T1653 T1651 nummod 18,Exon
R904 T1655 T1656 det the,first
R905 T1656 T1652 dobj first,encodes
R906 T1657 T1656 prep of,first
R907 T1658 T1659 det the,motifs
R908 T1659 T1657 pobj motifs,of
R909 T1660 T1659 nummod seven,motifs
R910 T1661 T1656 acl composing,first
R911 T1662 T1663 det the,domain
R912 T1663 T1661 dobj domain,composing
R913 T1664 T1663 amod conserved,domain
R914 T1665 T1666 npadvmod SNF2,like
R915 T1666 T1663 amod like,domain
R916 T1667 T1666 punct -,like
R917 T1668 T1663 prep of,domain
R918 T1669 T1668 pobj Atrx,of
R919 T1670 T1671 punct (,Figure
R920 T1671 T1652 parataxis Figure,encodes
R921 T1672 T1671 nummod 1,Figure
R922 T1673 T1671 punct ),Figure
R923 T1674 T1654 punct ;,shown
R924 T1675 T1654 nsubjpass mutation,shown
R925 T1676 T1675 prep of,mutation
R926 T1677 T1678 det the,motif
R927 T1678 T1676 pobj motif,of
R928 T1679 T1678 amod corresponding,motif
R929 T1680 T1678 prep of,motif
R930 T1681 T1682 det the,protein
R931 T1682 T1680 pobj protein,of
R932 T1683 T1682 compound yeast,protein
R933 T1684 T1682 compound SNF2,protein
R934 T1685 T1654 aux has,shown
R935 T1686 T1654 auxpass been,shown
R936 T1687 T1688 aux to,impair
R937 T1688 T1654 xcomp impair,shown
R938 T1689 T1688 advmod severely,impair
R939 T1690 T1691 compound SWI,SNF
R940 T1691 T1693 npadvmod SNF,dependent
R941 T1692 T1691 punct /,SNF
R942 T1693 T1695 amod dependent,expression
R943 T1694 T1693 punct -,dependent
R944 T1695 T1688 dobj expression,impair
R945 T1696 T1695 compound gene,expression
R946 T1697 T1698 punct [,12
R947 T1698 T1654 parataxis 12,shown
R948 T1699 T1698 punct ],12
R949 T1700 T1654 punct .,shown
R950 T1702 T1703 det The,failure
R951 T1703 T1704 nsubj failure,suggested
R952 T1705 T1706 aux to,recover
R953 T1706 T1703 acl recover,failure
R954 T1707 T1708 amod targeted,clones
R955 T1708 T1706 dobj clones,recover
R956 T1709 T1706 prep with,recover
R957 T1710 T1711 det these,vectors
R958 T1711 T1709 pobj vectors,with
R959 T1712 T1713 mark that,be
R960 T1713 T1704 advcl be,suggested
R961 T1714 T1713 nsubj Atrx,be
R962 T1715 T1713 aux may,be
R963 T1716 T1713 acomp important,be
R964 T1717 T1716 prep for,important
R965 T1718 T1719 amod normal,cell
R966 T1719 T1717 pobj cell,for
R967 T1720 T1719 compound ES,cell
R968 T1721 T1719 appos growth,cell
R969 T1722 T1721 cc and,growth
R970 T1723 T1721 conj expansion,growth
R971 T1724 T1713 cc and,be
R972 T1725 T1726 mark that,be
R973 T1726 T1713 conj be,be
R974 T1727 T1728 amod direct,targeting
R975 T1728 T1726 nsubj targeting,be
R976 T1729 T1728 prep of,targeting
R977 T1730 T1731 det the,locus
R978 T1731 T1729 pobj locus,of
R979 T1732 T1731 amod single,locus
R980 T1733 T1726 aux may,be
R981 T1734 T1726 neg not,be
R982 T1735 T1726 acomp possible,be
R983 T1736 T1704 punct .,suggested
R984 T1738 T1739 nsubj We,adopted
R985 T1740 T1739 advmod therefore,adopted
R986 T1741 T1742 det a,strategy
R987 T1742 T1739 dobj strategy,adopted
R988 T1743 T1742 amod conditional,strategy
R989 T1744 T1742 prep for,strategy
R990 T1745 T1744 pcomp targeting,for
R991 T1746 T1745 dobj exon,targeting
R992 T1747 T1746 nummod 18,exon
R993 T1748 T1749 punct (,Figure
R994 T1749 T1739 parataxis Figure,adopted
R995 T1750 T1749 nummod 2,Figure
R996 T1751 T1749 punct ),Figure
R997 T1752 T1739 cc and,adopted
R998 T1753 T1739 conj recovered,adopted
R999 T1754 T1755 nummod two,clones