PMC:1440874 JSON TXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id	Subject	Object	Predicate	Lexical cue
T152	0-4	NN	denotes	Loss
T154	0-104	sentence	denotes	Loss of Atrx Affects Trophoblast Development and the Pattern of X-Inactivation in Extraembryonic Tissues
T155	5-7	IN	denotes	of
T156	8-12	NN	denotes	Atrx
T153	13-20	VBZ	denotes	Affects
T157	21-32	NN	denotes	Trophoblast
T158	33-44	NN	denotes	Development
T159	45-48	CC	denotes	and
T160	49-52	DT	denotes	the
T161	53-60	NN	denotes	Pattern
T162	61-63	IN	denotes	of
T163	64-65	NN	denotes	X
T165	65-66	HYPH	denotes	-
T164	66-78	NN	denotes	Inactivation
T166	79-81	IN	denotes	in
T167	82-96	JJ	denotes	Extraembryonic
T168	97-104	NNS	denotes	Tissues
T169	104-105	sentence	denotes
T171	155-159	NN	denotes	ATRX
T170	155-304	sentence	denotes	ATRX is an X-encoded member of the SNF2 family of ATPase/helicase proteins thought to regulate gene expression by modifying chromatin at target loci.
T172	160-162	VBZ	denotes	is
T173	163-165	DT	denotes	an
T175	166-167	NN	denotes	X
T176	167-168	HYPH	denotes	-
T177	168-175	VBN	denotes	encoded
T174	176-182	NN	denotes	member
T178	183-185	IN	denotes	of
T179	186-189	DT	denotes	the
T181	190-194	NN	denotes	SNF2
T180	195-201	NN	denotes	family
T182	202-204	IN	denotes	of
T183	205-211	NN	denotes	ATPase
T185	211-212	HYPH	denotes	/
T184	212-220	NN	denotes	helicase
T186	221-229	NN	denotes	proteins
T187	230-237	VBN	denotes	thought
T188	238-240	TO	denotes	to
T189	241-249	VB	denotes	regulate
T190	250-254	NN	denotes	gene
T191	255-265	NN	denotes	expression
T192	266-268	IN	denotes	by
T193	269-278	VBG	denotes	modifying
T194	279-288	NN	denotes	chromatin
T195	289-291	IN	denotes	at
T196	292-298	NN	denotes	target
T197	299-303	NNS	denotes	loci
T198	303-304	.	denotes	.
T199	304-418	sentence	denotes	Mutations in ATRX provided the first example of a human genetic disease associated with defects in such proteins.
T200	305-314	NNS	denotes	Mutations
T202	315-317	IN	denotes	in
T203	318-322	NN	denotes	ATRX
T201	323-331	VBD	denotes	provided
T204	332-335	DT	denotes	the
T206	336-341	JJ	denotes	first
T205	342-349	NN	denotes	example
T207	350-352	IN	denotes	of
T208	353-354	DT	denotes	a
T210	355-360	JJ	denotes	human
T211	361-368	JJ	denotes	genetic
T209	369-376	NN	denotes	disease
T212	377-387	VBN	denotes	associated
T213	388-392	IN	denotes	with
T214	393-400	NNS	denotes	defects
T215	401-403	IN	denotes	in
T216	404-408	JJ	denotes	such
T217	409-417	NN	denotes	proteins
T218	417-418	.	denotes	.
T219	418-675	sentence	denotes	To better understand the role of ATRX in development and the associated abnormalities in the ATR-X (alpha thalassemia mental retardation, X-linked) syndrome, we conditionally inactivated the homolog in mice, Atrx, at the 8- to 16-cell stage of development.
T220	419-421	TO	denotes	To
T222	422-428	RBR	denotes	better
T221	429-439	VB	denotes	understand
T224	440-443	DT	denotes	the
T225	444-448	NN	denotes	role
T226	449-451	IN	denotes	of
T227	452-456	NN	denotes	ATRX
T228	457-459	IN	denotes	in
T229	460-471	NN	denotes	development
T230	472-475	CC	denotes	and
T231	476-479	DT	denotes	the
T233	480-490	VBN	denotes	associated
T232	491-504	NNS	denotes	abnormalities
T234	505-507	IN	denotes	in
T235	508-511	DT	denotes	the
T237	512-515	NN	denotes	ATR
T239	515-516	HYPH	denotes	-
T238	516-517	NN	denotes	X
T240	518-519	-LRB-	denotes	(
T241	519-524	NN	denotes	alpha
T242	525-536	NN	denotes	thalassemia
T243	537-543	JJ	denotes	mental
T244	544-555	NN	denotes	retardation
T245	555-557	,	denotes	,
T246	557-558	NN	denotes	X
T248	558-559	HYPH	denotes	-
T247	559-565	VBN	denotes	linked
T249	565-566	-RRB-	denotes	)
T236	567-575	NN	denotes	syndrome
T250	575-577	,	denotes	,
T251	577-579	PRP	denotes	we
T252	580-593	RB	denotes	conditionally
T223	594-605	VBD	denotes	inactivated
T253	606-609	DT	denotes	the
T254	610-617	NN	denotes	homolog
T255	618-620	IN	denotes	in
T256	621-625	NNS	denotes	mice
T257	625-627	,	denotes	,
T258	627-631	NN	denotes	Atrx
T259	631-633	,	denotes	,
T260	633-635	IN	denotes	at
T261	636-639	DT	denotes	the
T263	640-641	CD	denotes	8
T265	641-642	HYPH	denotes	-
T266	643-645	IN	denotes	to
T264	646-648	CD	denotes	16
T268	648-649	HYPH	denotes	-
T267	649-653	NN	denotes	cell
T262	654-659	NN	denotes	stage
T269	660-662	IN	denotes	of
T270	663-674	NN	denotes	development
T271	674-675	.	denotes	.
T272	675-977	sentence	denotes	The protein, Atrx, was ubiquitously expressed, and male embryos null for Atrx implanted and gastrulated normally but did not survive beyond 9.5 days postcoitus due to a defect in formation of the extraembryonic trophoblast, one of the first terminally differentiated lineages in the developing embryo.
T273	676-679	DT	denotes	The
T274	680-687	NN	denotes	protein
T276	687-689	,	denotes	,
T277	689-693	NN	denotes	Atrx
T278	693-695	,	denotes	,
T279	695-698	VBD	denotes	was
T280	699-711	RB	denotes	ubiquitously
T275	712-721	VBN	denotes	expressed
T281	721-723	,	denotes	,
T282	723-726	CC	denotes	and
T283	727-731	JJ	denotes	male
T284	732-739	NNS	denotes	embryos
T286	740-744	JJ	denotes	null
T287	745-748	IN	denotes	for
T288	749-753	NN	denotes	Atrx
T285	754-763	VBN	denotes	implanted
T289	764-767	CC	denotes	and
T290	768-779	VBN	denotes	gastrulated
T291	780-788	RB	denotes	normally
T292	789-792	CC	denotes	but
T293	793-796	VBD	denotes	did
T295	797-800	RB	denotes	not
T294	801-808	VB	denotes	survive
T296	809-815	IN	denotes	beyond
T297	816-819	CD	denotes	9.5
T298	820-824	NNS	denotes	days
T299	825-835	RB	denotes	postcoitus
T300	836-839	IN	denotes	due
T301	840-842	IN	denotes	to
T302	843-844	DT	denotes	a
T303	845-851	NN	denotes	defect
T304	852-854	IN	denotes	in
T305	855-864	NN	denotes	formation
T306	865-867	IN	denotes	of
T307	868-871	DT	denotes	the
T309	872-886	JJ	denotes	extraembryonic
T308	887-898	NN	denotes	trophoblast
T310	898-900	,	denotes	,
T311	900-903	CD	denotes	one
T312	904-906	IN	denotes	of
T313	907-910	DT	denotes	the
T315	911-916	JJ	denotes	first
T316	917-927	RB	denotes	terminally
T317	928-942	VBN	denotes	differentiated
T314	943-951	NNS	denotes	lineages
T318	952-954	IN	denotes	in
T319	955-958	DT	denotes	the
T321	959-969	VBG	denotes	developing
T320	970-976	NN	denotes	embryo
T322	976-977	.	denotes	.
T323	977-1136	sentence	denotes	Carrier female mice that inherit a maternal null allele should be affected, since the paternal X chromosome is normally inactivated in extraembryonic tissues.
T324	978-985	NN	denotes	Carrier
T326	986-992	JJ	denotes	female
T325	993-997	NNS	denotes	mice
T328	998-1002	WDT	denotes	that
T329	1003-1010	VBP	denotes	inherit
T330	1011-1012	DT	denotes	a
T332	1013-1021	JJ	denotes	maternal
T333	1022-1026	JJ	denotes	null
T331	1027-1033	NN	denotes	allele
T334	1034-1040	MD	denotes	should
T335	1041-1043	VB	denotes	be
T327	1044-1052	VBN	denotes	affected
T336	1052-1054	,	denotes	,
T337	1054-1059	IN	denotes	since
T339	1060-1063	DT	denotes	the
T341	1064-1072	JJ	denotes	paternal
T342	1073-1074	NN	denotes	X
T340	1075-1085	NN	denotes	chromosome
T338	1086-1088	VBZ	denotes	is
T343	1089-1097	RB	denotes	normally
T344	1098-1109	JJ	denotes	inactivated
T345	1110-1112	IN	denotes	in
T346	1113-1127	JJ	denotes	extraembryonic
T347	1128-1135	NNS	denotes	tissues
T348	1135-1136	.	denotes	.
T349	1136-1297	sentence	denotes	Surprisingly, however, some carrier females established a normal placenta and appeared to escape the usual pattern of imprinted X-inactivation in these tissues.
T350	1137-1149	RB	denotes	Surprisingly
T352	1149-1151	,	denotes	,
T353	1151-1158	RB	denotes	however
T354	1158-1160	,	denotes	,
T355	1160-1164	DT	denotes	some
T357	1165-1172	NN	denotes	carrier
T356	1173-1180	NNS	denotes	females
T351	1181-1192	VBD	denotes	established
T358	1193-1194	DT	denotes	a
T360	1195-1201	JJ	denotes	normal
T359	1202-1210	NN	denotes	placenta
T361	1211-1214	CC	denotes	and
T362	1215-1223	VBD	denotes	appeared
T363	1224-1226	TO	denotes	to
T364	1227-1233	VB	denotes	escape
T365	1234-1237	DT	denotes	the
T367	1238-1243	JJ	denotes	usual
T366	1244-1251	NN	denotes	pattern
T368	1252-1254	IN	denotes	of
T369	1255-1264	VBN	denotes	imprinted
T371	1265-1266	NN	denotes	X
T372	1266-1267	HYPH	denotes	-
T370	1267-1279	NN	denotes	inactivation
T373	1280-1282	IN	denotes	in
T374	1283-1288	DT	denotes	these
T375	1289-1296	NNS	denotes	tissues
T376	1296-1297	.	denotes	.
T377	1297-1506	sentence	denotes	Together these findings demonstrate an unexpected, specific, and essential role for Atrx in the development of the murine trophoblast and present an example of escape from imprinted X chromosome inactivation.
T378	1298-1306	RB	denotes	Together
T380	1307-1312	DT	denotes	these
T381	1313-1321	NNS	denotes	findings
T379	1322-1333	VBP	denotes	demonstrate
T382	1334-1336	DT	denotes	an
T384	1337-1347	JJ	denotes	unexpected
T385	1347-1349	,	denotes	,
T386	1349-1357	JJ	denotes	specific
T387	1357-1359	,	denotes	,
T388	1359-1362	CC	denotes	and
T389	1363-1372	JJ	denotes	essential
T383	1373-1377	NN	denotes	role
T390	1378-1381	IN	denotes	for
T391	1382-1386	NN	denotes	Atrx
T392	1387-1389	IN	denotes	in
T393	1390-1393	DT	denotes	the
T394	1394-1405	NN	denotes	development
T395	1406-1408	IN	denotes	of
T396	1409-1412	DT	denotes	the
T398	1413-1419	JJ	denotes	murine
T397	1420-1431	NN	denotes	trophoblast
T399	1432-1435	CC	denotes	and
T400	1436-1443	VBP	denotes	present
T401	1444-1446	DT	denotes	an
T402	1447-1454	NN	denotes	example
T403	1455-1457	IN	denotes	of
T404	1458-1464	NN	denotes	escape
T405	1465-1469	IN	denotes	from
T406	1470-1479	VBN	denotes	imprinted
T408	1480-1481	NN	denotes	X
T407	1482-1492	NN	denotes	chromosome
T409	1493-1505	NN	denotes	inactivation
T410	1505-1506	.	denotes	.
T722	2868-2871	NN	denotes	ATR
T724	2871-2872	HYPH	denotes	-
T723	2872-2873	NN	denotes	X
T725	2874-2882	NN	denotes	syndrome
T726	2883-2885	VBZ	denotes	is
T727	2886-2887	DT	denotes	a
T729	2888-2894	JJ	denotes	severe
T730	2894-2896	,	denotes	,
T731	2896-2910	JJ	denotes	nonprogressive
T728	2911-2915	NN	denotes	form
T732	2916-2918	IN	denotes	of
T733	2919-2920	NN	denotes	X
T735	2920-2921	HYPH	denotes	-
T734	2921-2927	VBN	denotes	linked
T737	2928-2934	JJ	denotes	mental
T736	2935-2946	NN	denotes	retardation
T738	2947-2951	WDT	denotes	that
T740	2952-2954	VBZ	denotes	is
T741	2955-2965	RB	denotes	frequently
T739	2966-2976	VBN	denotes	associated
T742	2977-2981	IN	denotes	with
T743	2982-2990	JJ	denotes	multiple
T745	2991-3001	JJ	denotes	congenital
T744	3002-3015	NNS	denotes	abnormalities
T746	3016-3017	-LRB-	denotes	[
T747	3017-3018	CD	denotes	1
T748	3018-3019	-RRB-	denotes	]
T749	3019-3020	.	denotes	.
T750	3020-3269	sentence	denotes	It is usually associated with a mild form of α-thalassaemia, caused by reduced expression of structurally intact α-globin genes, and characterised by the presence of β-globin tetramers (haemoglobin H inclusion bodies) in peripheral red blood cells.
T751	3021-3023	PRP	denotes	It
T753	3024-3026	VBZ	denotes	is
T754	3027-3034	RB	denotes	usually
T752	3035-3045	VBN	denotes	associated
T755	3046-3050	IN	denotes	with
T756	3051-3052	DT	denotes	a
T758	3053-3057	JJ	denotes	mild
T757	3058-3062	NN	denotes	form
T759	3063-3065	IN	denotes	of
T760	3066-3067	NN	denotes	α
T762	3067-3068	HYPH	denotes	-
T761	3068-3080	NN	denotes	thalassaemia
T763	3080-3082	,	denotes	,
T764	3082-3088	VBN	denotes	caused
T765	3089-3091	IN	denotes	by
T766	3092-3099	VBN	denotes	reduced
T767	3100-3110	NN	denotes	expression
T768	3111-3113	IN	denotes	of
T769	3114-3126	RB	denotes	structurally
T770	3127-3133	JJ	denotes	intact
T772	3134-3135	NN	denotes	α
T774	3135-3136	HYPH	denotes	-
T773	3136-3142	NN	denotes	globin
T771	3143-3148	NNS	denotes	genes
T775	3148-3150	,	denotes	,
T776	3150-3153	CC	denotes	and
T777	3154-3167	VBN	denotes	characterised
T778	3168-3170	IN	denotes	by
T779	3171-3174	DT	denotes	the
T780	3175-3183	NN	denotes	presence
T781	3184-3186	IN	denotes	of
T782	3187-3188	NN	denotes	β
T784	3188-3189	HYPH	denotes	-
T783	3189-3195	NN	denotes	globin
T785	3196-3205	NNS	denotes	tetramers
T786	3206-3207	-LRB-	denotes	(
T787	3207-3218	NN	denotes	haemoglobin
T788	3219-3220	NN	denotes	H
T790	3221-3230	NN	denotes	inclusion
T789	3231-3237	NNS	denotes	bodies
T791	3237-3238	-RRB-	denotes	)
T792	3239-3241	IN	denotes	in
T793	3242-3252	JJ	denotes	peripheral
T795	3253-3256	JJ	denotes	red
T796	3257-3262	NN	denotes	blood
T794	3263-3268	NNS	denotes	cells
T797	3268-3269	.	denotes	.
T798	3269-3389	sentence	denotes	Carrier females occasionally manifest haemoglobin H inclusions, but are otherwise intellectually and physically normal.
T799	3270-3277	NN	denotes	Carrier
T800	3278-3285	NNS	denotes	females
T802	3286-3298	RB	denotes	occasionally
T801	3299-3307	VBP	denotes	manifest
T803	3308-3319	NN	denotes	haemoglobin
T804	3320-3321	NN	denotes	H
T805	3322-3332	NNS	denotes	inclusions
T806	3332-3334	,	denotes	,
T807	3334-3337	CC	denotes	but
T808	3338-3341	VBP	denotes	are
T809	3342-3351	RB	denotes	otherwise
T810	3352-3366	RB	denotes	intellectually
T812	3367-3370	CC	denotes	and
T813	3371-3381	RB	denotes	physically
T811	3382-3388	JJ	denotes	normal
T814	3388-3389	.	denotes	.
T815	3389-3662	sentence	denotes	Studies of X-chromosome inactivation in carrier females have demonstrated preferential inactivation of the chromosome bearing the abnormal allele in a variety of tissues [2], and this skewing of X-inactivation is thought to explain the mild phenotype observed in carriers.
T816	3390-3397	NNS	denotes	Studies
T818	3398-3400	IN	denotes	of
T819	3401-3402	NN	denotes	X
T821	3402-3403	HYPH	denotes	-
T820	3403-3413	NN	denotes	chromosome
T822	3414-3426	NN	denotes	inactivation
T823	3427-3429	IN	denotes	in
T824	3430-3437	NN	denotes	carrier
T825	3438-3445	NNS	denotes	females
T826	3446-3450	VBP	denotes	have
T817	3451-3463	VBN	denotes	demonstrated
T827	3464-3476	JJ	denotes	preferential
T828	3477-3489	NN	denotes	inactivation
T829	3490-3492	IN	denotes	of
T830	3493-3496	DT	denotes	the
T831	3497-3507	NN	denotes	chromosome
T832	3508-3515	VBG	denotes	bearing
T833	3516-3519	DT	denotes	the
T835	3520-3528	JJ	denotes	abnormal
T834	3529-3535	NN	denotes	allele
T836	3536-3538	IN	denotes	in
T837	3539-3540	DT	denotes	a
T838	3541-3548	NN	denotes	variety
T839	3549-3551	IN	denotes	of
T840	3552-3559	NNS	denotes	tissues
T841	3560-3561	-LRB-	denotes	[
T842	3561-3562	CD	denotes	2
T843	3562-3563	-RRB-	denotes	]
T844	3563-3565	,	denotes	,
T845	3565-3568	CC	denotes	and
T846	3569-3573	DT	denotes	this
T847	3574-3581	NN	denotes	skewing
T849	3582-3584	IN	denotes	of
T850	3585-3586	NN	denotes	X
T852	3586-3587	HYPH	denotes	-
T851	3587-3599	NN	denotes	inactivation
T853	3600-3602	VBZ	denotes	is
T848	3603-3610	VBN	denotes	thought
T854	3611-3613	TO	denotes	to
T855	3614-3621	VB	denotes	explain
T856	3622-3625	DT	denotes	the
T858	3626-3630	JJ	denotes	mild
T857	3631-3640	NN	denotes	phenotype
T859	3641-3649	VBN	denotes	observed
T860	3650-3652	IN	denotes	in
T861	3653-3661	NNS	denotes	carriers
T862	3661-3662	.	denotes	.
T863	3662-3802	sentence	denotes	The ATR-X syndrome is caused by mutations in a gene (ATRX) that comprises 36 exons spanning 300 kb of genomic DNA at Chromosome Xq13.3 [3].
T864	3663-3666	DT	denotes	The
T866	3667-3670	NN	denotes	ATR
T868	3670-3671	HYPH	denotes	-
T867	3671-3672	NN	denotes	X
T865	3673-3681	NN	denotes	syndrome
T870	3682-3684	VBZ	denotes	is
T869	3685-3691	VBN	denotes	caused
T871	3692-3694	IN	denotes	by
T872	3695-3704	NNS	denotes	mutations
T873	3705-3707	IN	denotes	in
T874	3708-3709	DT	denotes	a
T875	3710-3714	NN	denotes	gene
T876	3715-3716	-LRB-	denotes	(
T877	3716-3720	NN	denotes	ATRX
T878	3720-3721	-RRB-	denotes	)
T879	3722-3726	WDT	denotes	that
T880	3727-3736	VBZ	denotes	comprises
T881	3737-3739	CD	denotes	36
T882	3740-3745	NNS	denotes	exons
T883	3746-3754	VBG	denotes	spanning
T884	3755-3758	CD	denotes	300
T885	3759-3761	NNS	denotes	kb
T886	3762-3764	IN	denotes	of
T887	3765-3772	JJ	denotes	genomic
T888	3773-3776	NN	denotes	DNA
T889	3777-3779	IN	denotes	at
T890	3780-3790	NN	denotes	Chromosome
T891	3791-3797	NN	denotes	Xq13.3
T892	3798-3799	-LRB-	denotes	[
T893	3799-3800	CD	denotes	3
T894	3800-3801	-RRB-	denotes	]
T895	3801-3802	.	denotes	.
T896	3802-3862	sentence	denotes	This gene encodes two dominant protein isoforms (Figure 1).
T897	3803-3807	DT	denotes	This
T898	3808-3812	NN	denotes	gene
T899	3813-3820	VBZ	denotes	encodes
T900	3821-3824	CD	denotes	two
T902	3825-3833	JJ	denotes	dominant
T903	3834-3841	NN	denotes	protein
T901	3842-3850	NNS	denotes	isoforms
T904	3851-3852	-LRB-	denotes	(
T905	3852-3858	NN	denotes	Figure
T906	3859-3860	CD	denotes	1
T907	3860-3861	-RRB-	denotes	)
T908	3861-3862	.	denotes	.
T909	3862-4211	sentence	denotes	As well as the full-length ATRX protein of ~280 kDa, which is encoded by a transcript of ~10 kb, we recently demonstrated that a truncated isoform called ATRXt (~200 kDa) is produced from a transcript of around 7 kb, which arises when intron 11 fails to be spliced from the primary transcript and an alternative intronic poly(A) signal is used [4].
T910	3863-3865	RB	denotes	As
T912	3866-3870	RB	denotes	well
T911	3871-3873	IN	denotes	as
T914	3874-3877	DT	denotes	the
T916	3878-3882	JJ	denotes	full
T918	3882-3883	HYPH	denotes	-
T917	3883-3889	NN	denotes	length
T919	3890-3894	NN	denotes	ATRX
T915	3895-3902	NN	denotes	protein
T920	3903-3905	IN	denotes	of
T921	3906-3907	SYM	denotes	~
T922	3907-3910	CD	denotes	280
T923	3911-3914	NN	denotes	kDa
T924	3914-3916	,	denotes	,
T925	3916-3921	WDT	denotes	which
T927	3922-3924	VBZ	denotes	is
T926	3925-3932	VBN	denotes	encoded
T928	3933-3935	IN	denotes	by
T929	3936-3937	DT	denotes	a
T930	3938-3948	NN	denotes	transcript
T931	3949-3951	IN	denotes	of
T932	3952-3953	SYM	denotes	~
T933	3953-3955	CD	denotes	10
T934	3956-3958	NN	denotes	kb
T935	3958-3960	,	denotes	,
T936	3960-3962	PRP	denotes	we
T937	3963-3971	RB	denotes	recently
T913	3972-3984	VBD	denotes	demonstrated
T938	3985-3989	IN	denotes	that
T940	3990-3991	DT	denotes	a
T942	3992-4001	VBN	denotes	truncated
T941	4002-4009	NN	denotes	isoform
T943	4010-4016	VBN	denotes	called
T944	4017-4022	NN	denotes	ATRXt
T945	4023-4024	-LRB-	denotes	(
T947	4024-4025	SYM	denotes	~
T948	4025-4028	CD	denotes	200
T946	4029-4032	NN	denotes	kDa
T949	4032-4033	-RRB-	denotes	)
T950	4034-4036	VBZ	denotes	is
T939	4037-4045	VBN	denotes	produced
T951	4046-4050	IN	denotes	from
T952	4051-4052	DT	denotes	a
T953	4053-4063	NN	denotes	transcript
T954	4064-4066	IN	denotes	of
T955	4067-4073	IN	denotes	around
T956	4074-4075	CD	denotes	7
T957	4076-4078	NN	denotes	kb
T958	4078-4080	,	denotes	,
T959	4080-4085	WDT	denotes	which
T960	4086-4092	VBZ	denotes	arises
T961	4093-4097	WRB	denotes	when
T963	4098-4104	NN	denotes	intron
T964	4105-4107	CD	denotes	11
T962	4108-4113	VBZ	denotes	fails
T965	4114-4116	TO	denotes	to
T967	4117-4119	VB	denotes	be
T966	4120-4127	VBN	denotes	spliced
T968	4128-4132	IN	denotes	from
T969	4133-4136	DT	denotes	the
T971	4137-4144	JJ	denotes	primary
T970	4145-4155	NN	denotes	transcript
T972	4156-4159	CC	denotes	and
T973	4160-4162	DT	denotes	an
T975	4163-4174	JJ	denotes	alternative
T976	4175-4183	JJ	denotes	intronic
T977	4184-4188	NN	denotes	poly
T978	4188-4189	-LRB-	denotes	(
T979	4189-4190	NN	denotes	A
T980	4190-4191	-RRB-	denotes	)
T974	4192-4198	NN	denotes	signal
T982	4199-4201	VBZ	denotes	is
T981	4202-4206	VBN	denotes	used
T983	4207-4208	-LRB-	denotes	[
T984	4208-4209	CD	denotes	4
T985	4209-4210	-RRB-	denotes	]
T986	4210-4211	.	denotes	.
T987	4211-4391	sentence	denotes	The mouse homolog of the ATRX gene, Atrx, is also situated on the X chromosome, and also gives rise to full-length (Atrx, ~280 kDa) and truncated (Atrxt, ~200 kDa) isoforms [4,5].
T988	4212-4215	DT	denotes	The
T990	4216-4221	NN	denotes	mouse
T989	4222-4229	NN	denotes	homolog
T992	4230-4232	IN	denotes	of
T993	4233-4236	DT	denotes	the
T995	4237-4241	NN	denotes	ATRX
T994	4242-4246	NN	denotes	gene
T996	4246-4248	,	denotes	,
T997	4248-4252	NN	denotes	Atrx
T998	4252-4254	,	denotes	,
T999	4254-4256	VBZ	denotes	is
T1000	4257-4261	RB	denotes	also
T991	4262-4270	VBN	denotes	situated
T1001	4271-4273	IN	denotes	on
T1002	4274-4277	DT	denotes	the
T1004	4278-4279	NN	denotes	X
T1003	4280-4290	NN	denotes	chromosome
T1005	4290-4292	,	denotes	,
T1006	4292-4295	CC	denotes	and
T1007	4296-4300	RB	denotes	also
T1008	4301-4306	VBZ	denotes	gives
T1009	4307-4311	NN	denotes	rise
T1010	4312-4314	IN	denotes	to
T1011	4315-4319	JJ	denotes	full
T1013	4319-4320	HYPH	denotes	-
T1012	4320-4326	NN	denotes	length
T1015	4327-4328	-LRB-	denotes	(
T1017	4328-4332	NN	denotes	Atrx
T1018	4332-4334	,	denotes	,
T1019	4334-4335	SYM	denotes	~
T1020	4335-4338	CD	denotes	280
T1016	4339-4342	NN	denotes	kDa
T1021	4342-4343	-RRB-	denotes	)
T1022	4344-4347	CC	denotes	and
T1023	4348-4357	VBN	denotes	truncated
T1024	4358-4359	-LRB-	denotes	(
T1026	4359-4364	NN	denotes	Atrxt
T1027	4364-4366	,	denotes	,
T1028	4366-4367	SYM	denotes	~
T1029	4367-4370	CD	denotes	200
T1025	4371-4374	NN	denotes	kDa
T1030	4374-4375	-RRB-	denotes	)
T1014	4376-4384	NNS	denotes	isoforms
T1031	4385-4386	-LRB-	denotes	[
T1033	4386-4387	CD	denotes	4
T1034	4387-4388	,	denotes	,
T1032	4388-4389	CD	denotes	5
T1035	4389-4390	-RRB-	denotes	]
T1036	4390-4391	.	denotes	.
T1037	4391-5401	sentence	denotes	Figure 1 Schematic Representation of the ATRX Isoforms Shown at the top is the human ATRX cDNA. The boxes represent the 36 exons. The introns are not to scale. The alternative splicing of exons 6 and 7 is indicated. Shown underneath are the two ATRX protein isoforms. Full-length ATRX (~280 kDa) is encoded by the largest open reading frame. The positions of the principal features (the PHD-like domain and the seven SWI/SNF-like motifs) are indicated. Above full-length ATRX is shown the truncated ATRXt isoform (apparent molecular weight of ~200 kDa) that arises through the failure to splice intron 11 and the use of an intronic poly(A) signal. The intron-encoded region of ATRXt is indicated as a filled grey box. Locations of recombinant proteins (A2, FXNP5, and H-300) used to generate antibodies are shown. The scale bar represents 200 amino acids. Disease-causing missense mutations are clustered in two regions of the gene: a PHD-like zinc finger domain and a SNF2-like ATPase domain (Figure 1) [6].
T1038	5249-5256	NN	denotes	Disease
T1040	5256-5257	HYPH	denotes	-
T1039	5257-5264	VBG	denotes	causing
T1042	5265-5273	NN	denotes	missense
T1041	5274-5283	NNS	denotes	mutations
T1044	5284-5287	VBP	denotes	are
T1043	5288-5297	VBN	denotes	clustered
T1045	5298-5300	IN	denotes	in
T1046	5301-5304	CD	denotes	two
T1047	5305-5312	NNS	denotes	regions
T1048	5313-5315	IN	denotes	of
T1049	5316-5319	DT	denotes	the
T1050	5320-5324	NN	denotes	gene
T1051	5324-5326	:	denotes	:
T1052	5326-5327	DT	denotes	a
T1054	5328-5331	NN	denotes	PHD
T1056	5331-5332	HYPH	denotes	-
T1055	5332-5336	JJ	denotes	like
T1057	5337-5341	NN	denotes	zinc
T1058	5342-5348	NN	denotes	finger
T1053	5349-5355	NN	denotes	domain
T1059	5356-5359	CC	denotes	and
T1060	5360-5361	DT	denotes	a
T1062	5362-5366	NN	denotes	SNF2
T1064	5366-5367	HYPH	denotes	-
T1063	5367-5371	JJ	denotes	like
T1065	5372-5378	NN	denotes	ATPase
T1061	5379-5385	NN	denotes	domain
T1066	5386-5387	-LRB-	denotes	(
T1067	5387-5393	NN	denotes	Figure
T1068	5394-5395	CD	denotes	1
T1069	5395-5396	-RRB-	denotes	)
T1070	5397-5398	-LRB-	denotes	[
T1071	5398-5399	CD	denotes	6
T1072	5399-5400	-RRB-	denotes	]
T1073	5400-5401	.	denotes	.
T1074	5401-5657	sentence	denotes	The former motif is thought to be involved in protein-protein interactions in chromatin [7], and the latter is a feature of chromatin-remodelling proteins, and the presence of disease-causing mutations indicates the functional importance of these domains.
T1075	5402-5405	DT	denotes	The
T1077	5406-5412	JJ	denotes	former
T1076	5413-5418	NN	denotes	motif
T1079	5419-5421	VBZ	denotes	is
T1078	5422-5429	VBN	denotes	thought
T1080	5430-5432	TO	denotes	to
T1082	5433-5435	VB	denotes	be
T1081	5436-5444	VBN	denotes	involved
T1083	5445-5447	IN	denotes	in
T1084	5448-5455	NN	denotes	protein
T1086	5455-5456	SYM	denotes	-
T1085	5456-5463	NN	denotes	protein
T1087	5464-5476	NNS	denotes	interactions
T1088	5477-5479	IN	denotes	in
T1089	5480-5489	NN	denotes	chromatin
T1090	5490-5491	-LRB-	denotes	[
T1091	5491-5492	CD	denotes	7
T1092	5492-5493	-RRB-	denotes	]
T1093	5493-5495	,	denotes	,
T1094	5495-5498	CC	denotes	and
T1095	5499-5502	DT	denotes	the
T1096	5503-5509	JJ	denotes	latter
T1097	5510-5512	VBZ	denotes	is
T1098	5513-5514	DT	denotes	a
T1099	5515-5522	NN	denotes	feature
T1100	5523-5525	IN	denotes	of
T1101	5526-5535	NN	denotes	chromatin
T1103	5535-5536	HYPH	denotes	-
T1102	5536-5547	VBG	denotes	remodelling
T1104	5548-5556	NN	denotes	proteins
T1105	5556-5558	,	denotes	,
T1106	5558-5561	CC	denotes	and
T1107	5562-5565	DT	denotes	the
T1108	5566-5574	NN	denotes	presence
T1110	5575-5577	IN	denotes	of
T1111	5578-5585	NN	denotes	disease
T1113	5585-5586	HYPH	denotes	-
T1112	5586-5593	VBG	denotes	causing
T1114	5594-5603	NNS	denotes	mutations
T1109	5604-5613	VBZ	denotes	indicates
T1115	5614-5617	DT	denotes	the
T1117	5618-5628	JJ	denotes	functional
T1116	5629-5639	NN	denotes	importance
T1118	5640-5642	IN	denotes	of
T1119	5643-5648	DT	denotes	these
T1120	5649-5656	NNS	denotes	domains
T1121	5656-5657	.	denotes	.
T1122	5657-5703	sentence	denotes	ATRX has been shown to remodel chromatin [8].
T1123	5658-5662	NN	denotes	ATRX
T1125	5663-5666	VBZ	denotes	has
T1126	5667-5671	VBN	denotes	been
T1124	5672-5677	VBN	denotes	shown
T1127	5678-5680	TO	denotes	to
T1128	5681-5688	VB	denotes	remodel
T1129	5689-5698	NN	denotes	chromatin
T1130	5699-5700	-LRB-	denotes	[
T1131	5700-5701	CD	denotes	8
T1132	5701-5702	-RRB-	denotes	]
T1133	5702-5703	.	denotes	.
T1134	5703-5846	sentence	denotes	It also interacts with HP1 at heterochromatin [9] and is recruited to promyelocytic leukemia nuclear bodies via an interaction with Daxx [10].
T1135	5704-5706	PRP	denotes	It
T1137	5707-5711	RB	denotes	also
T1136	5712-5721	VBZ	denotes	interacts
T1138	5722-5726	IN	denotes	with
T1139	5727-5730	NN	denotes	HP1
T1140	5731-5733	IN	denotes	at
T1141	5734-5749	NN	denotes	heterochromatin
T1142	5750-5751	-LRB-	denotes	[
T1143	5751-5752	CD	denotes	9
T1144	5752-5753	-RRB-	denotes	]
T1145	5754-5757	CC	denotes	and
T1146	5758-5760	VBZ	denotes	is
T1147	5761-5770	VBN	denotes	recruited
T1148	5771-5773	IN	denotes	to
T1149	5774-5787	JJ	denotes	promyelocytic
T1150	5788-5796	NN	denotes	leukemia
T1152	5797-5804	JJ	denotes	nuclear
T1151	5805-5811	NNS	denotes	bodies
T1153	5812-5815	IN	denotes	via
T1154	5816-5818	DT	denotes	an
T1155	5819-5830	NN	denotes	interaction
T1156	5831-5835	IN	denotes	with
T1157	5836-5840	NN	denotes	Daxx
T1158	5841-5842	-LRB-	denotes	[
T1159	5842-5844	CD	denotes	10
T1160	5844-5845	-RRB-	denotes	]
T1161	5845-5846	.	denotes	.
T1162	5846-5928	sentence	denotes	Furthermore, disruption of ATRX leads to diverse changes in DNA methylation [11].
T1163	5847-5858	RB	denotes	Furthermore
T1165	5858-5860	,	denotes	,
T1166	5860-5870	NN	denotes	disruption
T1167	5871-5873	IN	denotes	of
T1168	5874-5878	NN	denotes	ATRX
T1164	5879-5884	VBZ	denotes	leads
T1169	5885-5887	IN	denotes	to
T1170	5888-5895	JJ	denotes	diverse
T1171	5896-5903	NNS	denotes	changes
T1172	5904-5906	IN	denotes	in
T1173	5907-5910	NN	denotes	DNA
T1174	5911-5922	NN	denotes	methylation
T1175	5923-5924	-LRB-	denotes	[
T1176	5924-5926	CD	denotes	11
T1177	5926-5927	-RRB-	denotes	]
T1178	5927-5928	.	denotes	.
T1179	5928-5998	sentence	denotes	Nevertheless, the role ATRX plays in gene expression remains unclear.
T1180	5929-5941	RB	denotes	Nevertheless
T1182	5941-5943	,	denotes	,
T1183	5943-5946	DT	denotes	the
T1184	5947-5951	NN	denotes	role
T1185	5952-5956	NN	denotes	ATRX
T1186	5957-5962	VBZ	denotes	plays
T1187	5963-5965	IN	denotes	in
T1188	5966-5970	NN	denotes	gene
T1189	5971-5981	NN	denotes	expression
T1181	5982-5989	VBZ	denotes	remains
T1190	5990-5997	JJ	denotes	unclear
T1191	5997-5998	.	denotes	.
T1192	5998-6223	sentence	denotes	The consistent core of clinical and haematological features observed in ATR-X patients suggests that, like the SWI2/SNF2 chromatin-remodelling protein, ATRX probably regulates transcription of a discrete set of target genes.
T1193	5999-6002	DT	denotes	The
T1195	6003-6013	JJ	denotes	consistent
T1194	6014-6018	NN	denotes	core
T1197	6019-6021	IN	denotes	of
T1198	6022-6030	JJ	denotes	clinical
T1200	6031-6034	CC	denotes	and
T1201	6035-6049	JJ	denotes	haematological
T1199	6050-6058	NNS	denotes	features
T1202	6059-6067	VBN	denotes	observed
T1203	6068-6070	IN	denotes	in
T1204	6071-6074	NN	denotes	ATR
T1206	6074-6075	HYPH	denotes	-
T1205	6075-6076	NN	denotes	X
T1207	6077-6085	NNS	denotes	patients
T1196	6086-6094	VBZ	denotes	suggests
T1208	6095-6099	IN	denotes	that
T1210	6099-6101	,	denotes	,
T1211	6101-6105	IN	denotes	like
T1212	6106-6109	DT	denotes	the
T1214	6110-6114	NN	denotes	SWI2
T1216	6114-6115	HYPH	denotes	/
T1215	6115-6119	NN	denotes	SNF2
T1217	6120-6129	NN	denotes	chromatin
T1219	6129-6130	HYPH	denotes	-
T1218	6130-6141	VBG	denotes	remodelling
T1213	6142-6149	NN	denotes	protein
T1220	6149-6151	,	denotes	,
T1221	6151-6155	NN	denotes	ATRX
T1222	6156-6164	RB	denotes	probably
T1209	6165-6174	VBZ	denotes	regulates
T1223	6175-6188	NN	denotes	transcription
T1224	6189-6191	IN	denotes	of
T1225	6192-6193	DT	denotes	a
T1227	6194-6202	JJ	denotes	discrete
T1226	6203-6206	NN	denotes	set
T1228	6207-6209	IN	denotes	of
T1229	6210-6216	NN	denotes	target
T1230	6217-6222	NNS	denotes	genes
T1231	6222-6223	.	denotes	.
T1232	6223-6383	sentence	denotes	However, although there are clearly others to be found, at present the α-globin genes remain the only confirmed targets for transcriptional regulation by ATRX.
T1233	6224-6231	RB	denotes	However
T1235	6231-6233	,	denotes	,
T1236	6233-6241	IN	denotes	although
T1238	6242-6247	EX	denotes	there
T1237	6248-6251	VBP	denotes	are
T1239	6252-6259	RB	denotes	clearly
T1240	6260-6266	NNS	denotes	others
T1241	6267-6269	TO	denotes	to
T1243	6270-6272	VB	denotes	be
T1242	6273-6278	VBN	denotes	found
T1244	6278-6280	,	denotes	,
T1245	6280-6282	IN	denotes	at
T1246	6283-6290	JJ	denotes	present
T1247	6291-6294	DT	denotes	the
T1249	6295-6296	NN	denotes	α
T1251	6296-6297	HYPH	denotes	-
T1250	6297-6303	NN	denotes	globin
T1248	6304-6309	NNS	denotes	genes
T1234	6310-6316	VBP	denotes	remain
T1252	6317-6320	DT	denotes	the
T1254	6321-6325	RB	denotes	only
T1255	6326-6335	VBN	denotes	confirmed
T1253	6336-6343	NNS	denotes	targets
T1256	6344-6347	IN	denotes	for
T1257	6348-6363	JJ	denotes	transcriptional
T1258	6364-6374	NN	denotes	regulation
T1259	6375-6377	IN	denotes	by
T1260	6378-6382	NN	denotes	ATRX
T1261	6382-6383	.	denotes	.
T1262	6383-6482	sentence	denotes	Little is currently known about the precise role of the ATRX protein during mammalian development.
T1263	6384-6390	JJ	denotes	Little
T1265	6391-6393	VBZ	denotes	is
T1266	6394-6403	RB	denotes	currently
T1264	6404-6409	VBN	denotes	known
T1267	6410-6415	IN	denotes	about
T1268	6416-6419	DT	denotes	the
T1270	6420-6427	JJ	denotes	precise
T1269	6428-6432	NN	denotes	role
T1271	6433-6435	IN	denotes	of
T1272	6436-6439	DT	denotes	the
T1274	6440-6444	NN	denotes	ATRX
T1273	6445-6452	NN	denotes	protein
T1275	6453-6459	IN	denotes	during
T1276	6460-6469	JJ	denotes	mammalian
T1277	6470-6481	NN	denotes	development
T1278	6481-6482	.	denotes	.
T1279	6482-6777	sentence	denotes	To investigate the role of this protein during mouse development, we generated a conditionally deleted allele of the Atrx gene in mouse embryonic stem (ES) cells, and used these cells to examine the effect of ablating expression of the full-length Atrx protein in ES cells and in mouse embryos.
T1280	6483-6485	TO	denotes	To
T1281	6486-6497	VB	denotes	investigate
T1283	6498-6501	DT	denotes	the
T1284	6502-6506	NN	denotes	role
T1285	6507-6509	IN	denotes	of
T1286	6510-6514	DT	denotes	this
T1287	6515-6522	NN	denotes	protein
T1288	6523-6529	IN	denotes	during
T1289	6530-6535	NN	denotes	mouse
T1290	6536-6547	NN	denotes	development
T1291	6547-6549	,	denotes	,
T1292	6549-6551	PRP	denotes	we
T1282	6552-6561	VBD	denotes	generated
T1293	6562-6563	DT	denotes	a
T1295	6564-6577	RB	denotes	conditionally
T1296	6578-6585	VBN	denotes	deleted
T1294	6586-6592	NN	denotes	allele
T1297	6593-6595	IN	denotes	of
T1298	6596-6599	DT	denotes	the
T1300	6600-6604	NN	denotes	Atrx
T1299	6605-6609	NN	denotes	gene
T1301	6610-6612	IN	denotes	in
T1302	6613-6618	NN	denotes	mouse
T1304	6619-6628	JJ	denotes	embryonic
T1305	6629-6633	NN	denotes	stem
T1306	6634-6635	-LRB-	denotes	(
T1307	6635-6637	NN	denotes	ES
T1308	6637-6638	-RRB-	denotes	)
T1303	6639-6644	NNS	denotes	cells
T1309	6644-6646	,	denotes	,
T1310	6646-6649	CC	denotes	and
T1311	6650-6654	VBD	denotes	used
T1312	6655-6660	DT	denotes	these
T1313	6661-6666	NNS	denotes	cells
T1314	6667-6669	TO	denotes	to
T1315	6670-6677	VB	denotes	examine
T1316	6678-6681	DT	denotes	the
T1317	6682-6688	NN	denotes	effect
T1318	6689-6691	IN	denotes	of
T1319	6692-6700	VBG	denotes	ablating
T1320	6701-6711	NN	denotes	expression
T1321	6712-6714	IN	denotes	of
T1322	6715-6718	DT	denotes	the
T1324	6719-6723	JJ	denotes	full
T1326	6723-6724	HYPH	denotes	-
T1325	6724-6730	NN	denotes	length
T1327	6731-6735	NN	denotes	Atrx
T1323	6736-6743	NN	denotes	protein
T1328	6744-6746	IN	denotes	in
T1329	6747-6749	NN	denotes	ES
T1330	6750-6755	NNS	denotes	cells
T1331	6756-6759	CC	denotes	and
T1332	6760-6762	IN	denotes	in
T1333	6763-6768	NN	denotes	mouse
T1334	6769-6776	NNS	denotes	embryos
T1335	6776-6777	.	denotes	.
T1574	6788-6798	NN	denotes	Generation
T1575	6799-6801	IN	denotes	of
T1576	6802-6804	NN	denotes	ES
T1577	6805-6810	NNS	denotes	Cells
T1578	6811-6818	VBG	denotes	Lacking
T1579	6819-6823	JJ	denotes	Full
T1581	6823-6824	HYPH	denotes	-
T1580	6824-6830	NN	denotes	Length
T1582	6831-6835	NN	denotes	Atrx
T1583	6835-7014	sentence	denotes	Like the human gene, the mouse Atrx gene is also X-linked, such that a direct disruption of the single Atrx allele in male ES cells would immediately give rise to the null state.
T1584	6836-6840	IN	denotes	Like
T1586	6841-6844	DT	denotes	the
T1588	6845-6850	JJ	denotes	human
T1587	6851-6855	NN	denotes	gene
T1589	6855-6857	,	denotes	,
T1590	6857-6860	DT	denotes	the
T1592	6861-6866	NN	denotes	mouse
T1593	6867-6871	NN	denotes	Atrx
T1591	6872-6876	NN	denotes	gene
T1585	6877-6879	VBZ	denotes	is
T1594	6880-6884	RB	denotes	also
T1595	6885-6886	NN	denotes	X
T1597	6886-6887	HYPH	denotes	-
T1596	6887-6893	VBN	denotes	linked
T1598	6893-6895	,	denotes	,
T1599	6895-6899	JJ	denotes	such
T1601	6900-6904	IN	denotes	that
T1602	6905-6906	DT	denotes	a
T1604	6907-6913	JJ	denotes	direct
T1603	6914-6924	NN	denotes	disruption
T1605	6925-6927	IN	denotes	of
T1606	6928-6931	DT	denotes	the
T1608	6932-6938	JJ	denotes	single
T1609	6939-6943	NN	denotes	Atrx
T1607	6944-6950	NN	denotes	allele
T1610	6951-6953	IN	denotes	in
T1611	6954-6958	JJ	denotes	male
T1613	6959-6961	NN	denotes	ES
T1612	6962-6967	NNS	denotes	cells
T1614	6968-6973	MD	denotes	would
T1615	6974-6985	RB	denotes	immediately
T1600	6986-6990	VB	denotes	give
T1616	6991-6995	NN	denotes	rise
T1617	6996-6998	IN	denotes	to
T1618	6999-7002	DT	denotes	the
T1620	7003-7007	JJ	denotes	null
T1619	7008-7013	NN	denotes	state
T1621	7013-7014	.	denotes	.
T1622	7014-7181	sentence	denotes	No targeted clones were recovered after attempted homologous recombination in male E14TG2a ES cells using two different vectors that removed exon 18 of the Atrx gene.
T1623	7015-7017	DT	denotes	No
T1625	7018-7026	VBN	denotes	targeted
T1624	7027-7033	NNS	denotes	clones
T1627	7034-7038	VBD	denotes	were
T1626	7039-7048	VBN	denotes	recovered
T1628	7049-7054	IN	denotes	after
T1629	7055-7064	VBN	denotes	attempted
T1631	7065-7075	JJ	denotes	homologous
T1630	7076-7089	NN	denotes	recombination
T1632	7090-7092	IN	denotes	in
T1633	7093-7097	JJ	denotes	male
T1635	7098-7105	NN	denotes	E14TG2a
T1636	7106-7108	NN	denotes	ES
T1634	7109-7114	NNS	denotes	cells
T1637	7115-7120	VBG	denotes	using
T1638	7121-7124	CD	denotes	two
T1640	7125-7134	JJ	denotes	different
T1639	7135-7142	NNS	denotes	vectors
T1641	7143-7147	WDT	denotes	that
T1642	7148-7155	VBD	denotes	removed
T1643	7156-7160	NN	denotes	exon
T1644	7161-7163	CD	denotes	18
T1645	7164-7166	IN	denotes	of
T1646	7167-7170	DT	denotes	the
T1648	7171-7175	NN	denotes	Atrx
T1647	7176-7180	NN	denotes	gene
T1649	7180-7181	.	denotes	.
T1650	7181-7424	sentence	denotes	Exon 18 encodes the first of the seven motifs composing the conserved SNF2-like domain of Atrx (Figure 1); mutation of the corresponding motif of the yeast SNF2 protein has been shown to severely impair SWI/SNF-dependent gene expression [12].
T1651	7182-7186	NN	denotes	Exon
T1653	7187-7189	CD	denotes	18
T1652	7190-7197	VBZ	denotes	encodes
T1655	7198-7201	DT	denotes	the
T1656	7202-7207	JJ	denotes	first
T1657	7208-7210	IN	denotes	of
T1658	7211-7214	DT	denotes	the
T1660	7215-7220	CD	denotes	seven
T1659	7221-7227	NNS	denotes	motifs
T1661	7228-7237	VBG	denotes	composing
T1662	7238-7241	DT	denotes	the
T1664	7242-7251	VBN	denotes	conserved
T1665	7252-7256	NN	denotes	SNF2
T1667	7256-7257	HYPH	denotes	-
T1666	7257-7261	JJ	denotes	like
T1663	7262-7268	NN	denotes	domain
T1668	7269-7271	IN	denotes	of
T1669	7272-7276	NN	denotes	Atrx
T1670	7277-7278	-LRB-	denotes	(
T1671	7278-7284	NN	denotes	Figure
T1672	7285-7286	CD	denotes	1
T1673	7286-7287	-RRB-	denotes	)
T1674	7287-7288	:	denotes	;
T1675	7289-7297	NN	denotes	mutation
T1676	7298-7300	IN	denotes	of
T1677	7301-7304	DT	denotes	the
T1679	7305-7318	VBG	denotes	corresponding
T1678	7319-7324	NN	denotes	motif
T1680	7325-7327	IN	denotes	of
T1681	7328-7331	DT	denotes	the
T1683	7332-7337	NN	denotes	yeast
T1684	7338-7342	NN	denotes	SNF2
T1682	7343-7350	NN	denotes	protein
T1685	7351-7354	VBZ	denotes	has
T1686	7355-7359	VBN	denotes	been
T1654	7360-7365	VBN	denotes	shown
T1687	7366-7368	TO	denotes	to
T1689	7369-7377	RB	denotes	severely
T1688	7378-7384	VB	denotes	impair
T1690	7385-7388	NN	denotes	SWI
T1692	7388-7389	HYPH	denotes	/
T1691	7389-7392	NN	denotes	SNF
T1694	7392-7393	HYPH	denotes	-
T1693	7393-7402	JJ	denotes	dependent
T1696	7403-7407	NN	denotes	gene
T1695	7408-7418	NN	denotes	expression
T1697	7419-7420	-LRB-	denotes	[
T1698	7420-7422	CD	denotes	12
T1699	7422-7423	-RRB-	denotes	]
T1700	7423-7424	.	denotes	.
T1701	7424-7626	sentence	denotes	The failure to recover targeted clones with these vectors suggested that Atrx may be important for normal ES cell growth and expansion and that direct targeting of the single locus may not be possible.
T1702	7425-7428	DT	denotes	The
T1703	7429-7436	NN	denotes	failure
T1705	7437-7439	TO	denotes	to
T1706	7440-7447	VB	denotes	recover
T1707	7448-7456	VBN	denotes	targeted
T1708	7457-7463	NNS	denotes	clones
T1709	7464-7468	IN	denotes	with
T1710	7469-7474	DT	denotes	these
T1711	7475-7482	NNS	denotes	vectors
T1704	7483-7492	VBD	denotes	suggested
T1712	7493-7497	IN	denotes	that
T1714	7498-7502	NN	denotes	Atrx
T1715	7503-7506	MD	denotes	may
T1713	7507-7509	VB	denotes	be
T1716	7510-7519	JJ	denotes	important
T1717	7520-7523	IN	denotes	for
T1718	7524-7530	JJ	denotes	normal
T1720	7531-7533	NN	denotes	ES
T1719	7534-7538	NN	denotes	cell
T1721	7539-7545	NN	denotes	growth
T1722	7546-7549	CC	denotes	and
T1723	7550-7559	NN	denotes	expansion
T1724	7560-7563	CC	denotes	and
T1725	7564-7568	IN	denotes	that
T1727	7569-7575	JJ	denotes	direct
T1728	7576-7585	NN	denotes	targeting
T1729	7586-7588	IN	denotes	of
T1730	7589-7592	DT	denotes	the
T1732	7593-7599	JJ	denotes	single
T1731	7600-7605	NN	denotes	locus
T1733	7606-7609	MD	denotes	may
T1734	7610-7613	RB	denotes	not
T1726	7614-7616	VB	denotes	be
T1735	7617-7625	JJ	denotes	possible
T1736	7625-7626	.	denotes	.
T1737	7626-7857	sentence	denotes	We therefore adopted a conditional strategy for targeting exon 18 (Figure 2) and recovered two clones in which exon 18 has been flanked by loxP recognition sites for the Cre recombinase (Atrx flox allele in Figure 2A) (Figure 2B).
T1738	7627-7629	PRP	denotes	We
T1740	7630-7639	RB	denotes	therefore
T1739	7640-7647	VBD	denotes	adopted
T1741	7648-7649	DT	denotes	a
T1743	7650-7661	JJ	denotes	conditional
T1742	7662-7670	NN	denotes	strategy
T1744	7671-7674	IN	denotes	for
T1745	7675-7684	VBG	denotes	targeting
T1746	7685-7689	NN	denotes	exon
T1747	7690-7692	CD	denotes	18
T1748	7693-7694	-LRB-	denotes	(
T1749	7694-7700	NN	denotes	Figure
T1750	7701-7702	CD	denotes	2
T1751	7702-7703	-RRB-	denotes	)
T1752	7704-7707	CC	denotes	and
T1753	7708-7717	VBD	denotes	recovered
T1754	7718-7721	CD	denotes	two
T1755	7722-7728	NNS	denotes	clones
T1756	7729-7731	IN	denotes	in
T1758	7732-7737	WDT	denotes	which
T1759	7738-7742	NN	denotes	exon
T1760	7743-7745	CD	denotes	18
T1761	7746-7749	VBZ	denotes	has
T1762	7750-7754	VBN	denotes	been
T1757	7755-7762	VBN	denotes	flanked
T1763	7763-7765	IN	denotes	by
T1764	7766-7770	NN	denotes	loxP
T1766	7771-7782	NN	denotes	recognition
T1765	7783-7788	NNS	denotes	sites
T1767	7789-7792	IN	denotes	for
T1768	7793-7796	DT	denotes	the
T1770	7797-7800	NN	denotes	Cre
T1769	7801-7812	NN	denotes	recombinase
T1771	7813-7814	-LRB-	denotes	(
T1773	7814-7823	NN	denotes	Atrx flox
T1772	7824-7830	NN	denotes	allele
T1774	7831-7833	IN	denotes	in
T1775	7834-7840	NN	denotes	Figure
T1776	7841-7843	NN	denotes	2A
T1777	7843-7844	-RRB-	denotes	)
T1778	7845-7846	-LRB-	denotes	(
T1780	7846-7852	NN	denotes	Figure
T1779	7853-7855	NN	denotes	2B
T1781	7855-7856	-RRB-	denotes	)
T1782	7856-7857	.	denotes	.
T1783	7857-7942	sentence	denotes	This allele also contains a loxP-flanked MC1-neor cassette in intron 17 (Figure 2A).
T1784	7858-7862	DT	denotes	This
T1785	7863-7869	NN	denotes	allele
T1787	7870-7874	RB	denotes	also
T1786	7875-7883	VBZ	denotes	contains
T1788	7884-7885	DT	denotes	a
T1790	7886-7890	NN	denotes	loxP
T1792	7890-7891	HYPH	denotes	-
T1791	7891-7898	VBN	denotes	flanked
T1793	7899-7902	NN	denotes	MC1
T1795	7902-7903	HYPH	denotes	-
T1794	7903-7907	NN	denotes	neor
T1789	7908-7916	NN	denotes	cassette
T1796	7917-7919	IN	denotes	in
T1797	7920-7926	NN	denotes	intron
T1798	7927-7929	CD	denotes	17
T1799	7930-7931	-LRB-	denotes	(
T1801	7931-7937	NN	denotes	Figure
T1800	7938-7940	NN	denotes	2A
T1802	7940-7941	-RRB-	denotes	)
T1803	7941-7942	.	denotes	.
T1804	7942-8116	sentence	denotes	Northern and Western blot analyses (Figure 2D and 2E) confirmed that the Atrx flox clones continued to express both full-length Atrx protein and the truncated Atrxt isoform.
T1805	7943-7951	NNP	denotes	Northern
T1807	7952-7955	CC	denotes	and
T1808	7956-7963	NNP	denotes	Western
T1806	7964-7968	NN	denotes	blot
T1809	7969-7977	NNS	denotes	analyses
T1811	7978-7979	-LRB-	denotes	(
T1813	7979-7985	NN	denotes	Figure
T1812	7986-7988	NN	denotes	2D
T1814	7989-7992	CC	denotes	and
T1815	7993-7995	NN	denotes	2E
T1816	7995-7996	-RRB-	denotes	)
T1810	7997-8006	VBD	denotes	confirmed
T1817	8007-8011	IN	denotes	that
T1819	8012-8015	DT	denotes	the
T1821	8016-8025	NN	denotes	Atrx flox
T1820	8026-8032	NNS	denotes	clones
T1818	8033-8042	VBD	denotes	continued
T1822	8043-8045	TO	denotes	to
T1823	8046-8053	VB	denotes	express
T1824	8054-8058	CC	denotes	both
T1826	8059-8063	JJ	denotes	full
T1828	8063-8064	HYPH	denotes	-
T1827	8064-8070	NN	denotes	length
T1829	8071-8075	NN	denotes	Atrx
T1825	8076-8083	NN	denotes	protein
T1830	8084-8087	CC	denotes	and
T1831	8088-8091	DT	denotes	the
T1833	8092-8101	VBN	denotes	truncated
T1834	8102-8107	NN	denotes	Atrxt
T1832	8108-8115	NN	denotes	isoform
T1835	8115-8116	.	denotes	.
T1836	8116-10848	sentence	denotes	Figure 2 Cre-Mediated Ablation of Full-Length Atrx Protein in ES Cells (A) Strategy for targeted deletion of exon 18 of the Atrx gene. The top line shows the wild-type allele (Atrx WT) at the region surrounding exon 18. Below is shown the targeting vector and the targeted allele (Atrx flox) resulting from homologous recombination. The loxP target sites of the Cre recombinase are shown as black triangles, and the three possible recombination events that can be mediated by the Cre recombinase are indicated (labelled A, B, and C in the Atrx flox allele). At bottom is shown the Cre-recombined allele (Atrx Δ18Δneo) (resulting from recombination event C) in which both exon 18 and the MC1neopA selection cassette have been deleted. EcoRI (labelled E) and SacI (labelled S) sites present on the targeted 129 strain X chromosome are indicated. Black bars indicate the positions of the probes used in Southern blots. (B) Southern blot analysis of EcoRI-digested DNA from either wild-type ES cells (E14) or targeted ES cell clones bearing the Atrx flox allele (1/F12 and 1/G11) hybridised with either the 20/27 (left blot) or Hae0.9 (right blot) probes. The EcoRI fragment of the Atrx WT allele (18.5 kb) has been replaced with the expected fragments of 11.2 kb (20/27 probe) or 8.5 kb (Hae0.9 probe) (C) Southern blot analysis of SacI-digested DNA from either wild-type ES cells (E14) or targeted ES cell clones bearing the Atrx flox allele (1/F12 and 1/G11) or Cre-recombinant clones derived from these (1/F12B1F12 and 1/G11D5). The membrane was hybridised with the intron 17 probe indicated in (A). The expected bands of 6.2 (Atrx WT), 5.0 (Atrx flox), and 2.8 (Atrx Δ18Δneo) kb were observed. (D) Northern blot analysis of RNA from ES cells shown in (C). The membrane was hybridised first to a probe from exon 10 of the Atrx gene (top blot) and subsequently to a β-actin cDNA probe as loading control (bottom blot). The transcripts responsible for full-length Atrx (~10 kb) and the truncated Atrxt isoforms (~7 kb) are indicated. (E) Western blot analysis of whole-cell extracts from the clones shown in (C) using an anti-ATRX monoclonal antibody (23C, raised against peptide A2 of the human ATRX protein shown in Figure 1). The full-length and truncated Atrx isoforms are indicated. To generate the full deletion in ES cells, the Atrx flox clones (1/F12 and 1/G11) were transiently transfected with a Cre-recombinase expression plasmid (pCAGGS-Cre-IRESpuro), and subclones were recovered bearing an allele (Atrx Δ18Δneo in Figure 2A) in which both exon 18 and the neor cassette had been deleted by the Cre recombinase (resulting from the recombination event labelled “C” in the Atrx flox allele shown in Figure 2A) (Figure 2C).
T1837	10404-10406	TO	denotes	To
T1838	10407-10415	VB	denotes	generate
T1840	10416-10419	DT	denotes	the
T1842	10420-10424	JJ	denotes	full
T1841	10425-10433	NN	denotes	deletion
T1843	10434-10436	IN	denotes	in
T1844	10437-10439	NN	denotes	ES
T1845	10440-10445	NNS	denotes	cells
T1846	10445-10447	,	denotes	,
T1847	10447-10450	DT	denotes	the
T1849	10451-10460	NN	denotes	Atrx flox
T1848	10461-10467	NNS	denotes	clones
T1850	10468-10469	-LRB-	denotes	(
T1852	10469-10470	CD	denotes	1
T1853	10470-10471	HYPH	denotes	/
T1851	10471-10474	NN	denotes	F12
T1854	10475-10478	CC	denotes	and
T1855	10479-10480	CD	denotes	1
T1857	10480-10481	HYPH	denotes	/
T1856	10481-10484	NN	denotes	G11
T1858	10484-10485	-RRB-	denotes	)
T1859	10486-10490	VBD	denotes	were
T1860	10491-10502	RB	denotes	transiently
T1839	10503-10514	VBN	denotes	transfected
T1861	10515-10519	IN	denotes	with
T1862	10520-10521	DT	denotes	a
T1864	10522-10525	NN	denotes	Cre
T1866	10525-10526	HYPH	denotes	-
T1865	10526-10537	NN	denotes	recombinase
T1867	10538-10548	NN	denotes	expression
T1863	10549-10556	NN	denotes	plasmid
T1868	10557-10558	-LRB-	denotes	(
T1869	10558-10564	NN	denotes	pCAGGS
T1871	10564-10565	HYPH	denotes	-
T1872	10565-10568	NN	denotes	Cre
T1873	10568-10569	HYPH	denotes	-
T1870	10569-10577	NN	denotes	IRESpuro
T1874	10577-10578	-RRB-	denotes	)
T1875	10578-10580	,	denotes	,
T1876	10580-10583	CC	denotes	and
T1877	10584-10593	NNS	denotes	subclones
T1879	10594-10598	VBD	denotes	were
T1880	10599-10608	VBN	denotes	recovered
T1878	10609-10616	VBG	denotes	bearing
T1881	10617-10619	DT	denotes	an
T1882	10620-10626	NN	denotes	allele
T1883	10627-10628	-LRB-	denotes	(
T1884	10628-10640	NN	denotes	Atrx Δ18Δneo
T1885	10641-10643	IN	denotes	in
T1886	10644-10650	NN	denotes	Figure
T1887	10651-10653	NN	denotes	2A
T1888	10653-10654	-RRB-	denotes	)
T1889	10655-10657	IN	denotes	in
T1891	10658-10663	WDT	denotes	which
T1892	10664-10668	CC	denotes	both
T1893	10669-10673	NN	denotes	exon
T1894	10674-10676	CD	denotes	18
T1895	10677-10680	CC	denotes	and
T1896	10681-10684	DT	denotes	the
T1898	10685-10689	NN	denotes	neor
T1897	10690-10698	NN	denotes	cassette
T1899	10699-10702	VBD	denotes	had
T1900	10703-10707	VBN	denotes	been
T1890	10708-10715	VBN	denotes	deleted
T1901	10716-10718	IN	denotes	by
T1902	10719-10722	DT	denotes	the
T1904	10723-10726	NN	denotes	Cre
T1903	10727-10738	NN	denotes	recombinase
T1905	10739-10740	-LRB-	denotes	(
T1906	10740-10749	VBG	denotes	resulting
T1907	10750-10754	IN	denotes	from
T1908	10755-10758	DT	denotes	the
T1910	10759-10772	NN	denotes	recombination
T1909	10773-10778	NN	denotes	event
T1911	10779-10787	VBN	denotes	labelled
T1912	10788-10789	``	denotes	“
T1913	10789-10790	NN	denotes	C
T1914	10790-10791	''	denotes	”
T1915	10792-10794	IN	denotes	in
T1916	10795-10798	DT	denotes	the
T1918	10799-10808	NN	denotes	Atrx flox
T1917	10809-10815	NN	denotes	allele
T1919	10816-10821	VBN	denotes	shown
T1920	10822-10824	IN	denotes	in
T1921	10825-10831	NN	denotes	Figure
T1922	10832-10834	NN	denotes	2A
T1923	10834-10835	-RRB-	denotes	)
T1924	10836-10837	-LRB-	denotes	(
T1926	10837-10843	NN	denotes	Figure
T1925	10844-10846	NN	denotes	2C
T1927	10846-10847	-RRB-	denotes	)
T1928	10847-10848	.	denotes	.
T1929	10848-11129	sentence	denotes	Northern and Western blot analyses (Figure 2D and 2E) revealed that the full-length Atrx transcript and protein is completely abolished in the Atrx Δ18Δneo recombinant clones, suggesting that deletion of this region has a highly destabilising effect on the full-length transcript.
T1930	10849-10857	NNP	denotes	Northern
T1932	10858-10861	CC	denotes	and
T1933	10862-10869	NNP	denotes	Western
T1931	10870-10874	NN	denotes	blot
T1934	10875-10883	NNS	denotes	analyses
T1936	10884-10885	-LRB-	denotes	(
T1938	10885-10891	NN	denotes	Figure
T1937	10892-10894	NN	denotes	2D
T1939	10895-10898	CC	denotes	and
T1940	10899-10901	NN	denotes	2E
T1941	10901-10902	-RRB-	denotes	)
T1935	10903-10911	VBD	denotes	revealed
T1942	10912-10916	IN	denotes	that
T1944	10917-10920	DT	denotes	the
T1946	10921-10925	JJ	denotes	full
T1948	10925-10926	HYPH	denotes	-
T1947	10926-10932	NN	denotes	length
T1949	10933-10937	NN	denotes	Atrx
T1945	10938-10948	NN	denotes	transcript
T1950	10949-10952	CC	denotes	and
T1951	10953-10960	NN	denotes	protein
T1952	10961-10963	VBZ	denotes	is
T1953	10964-10974	RB	denotes	completely
T1943	10975-10984	VBN	denotes	abolished
T1954	10985-10987	IN	denotes	in
T1955	10988-10991	DT	denotes	the
T1957	10992-11004	NN	denotes	Atrx Δ18Δneo
T1958	11005-11016	JJ	denotes	recombinant
T1956	11017-11023	NNS	denotes	clones
T1959	11023-11025	,	denotes	,
T1960	11025-11035	VBG	denotes	suggesting
T1961	11036-11040	IN	denotes	that
T1963	11041-11049	NN	denotes	deletion
T1964	11050-11052	IN	denotes	of
T1965	11053-11057	DT	denotes	this
T1966	11058-11064	NN	denotes	region
T1962	11065-11068	VBZ	denotes	has
T1967	11069-11070	DT	denotes	a
T1969	11071-11077	RB	denotes	highly
T1970	11078-11091	JJ	denotes	destabilising
T1968	11092-11098	NN	denotes	effect
T1971	11099-11101	IN	denotes	on
T1972	11102-11105	DT	denotes	the
T1974	11106-11110	JJ	denotes	full
T1976	11110-11111	HYPH	denotes	-
T1975	11111-11117	NN	denotes	length
T1973	11118-11128	NN	denotes	transcript
T1977	11128-11129	.	denotes	.
T1978	11129-11285	sentence	denotes	As expected, the truncated Atrxt isoform, the transcript of which is terminated within intron 11 [4], is unaffected by the deletion of exon 18 (Figure 2E).
T1979	11130-11132	IN	denotes	As
T1980	11133-11141	VBN	denotes	expected
T1982	11141-11143	,	denotes	,
T1983	11143-11146	DT	denotes	the
T1985	11147-11156	VBN	denotes	truncated
T1986	11157-11162	NN	denotes	Atrxt
T1984	11163-11170	NN	denotes	isoform
T1987	11170-11172	,	denotes	,
T1988	11172-11175	DT	denotes	the
T1989	11176-11186	NN	denotes	transcript
T1991	11187-11189	IN	denotes	of
T1992	11190-11195	WDT	denotes	which
T1993	11196-11198	VBZ	denotes	is
T1990	11199-11209	VBN	denotes	terminated
T1994	11210-11216	IN	denotes	within
T1995	11217-11223	NN	denotes	intron
T1996	11224-11226	CD	denotes	11
T1997	11227-11228	-LRB-	denotes	[
T1998	11228-11229	CD	denotes	4
T1999	11229-11230	-RRB-	denotes	]
T2000	11230-11232	,	denotes	,
T1981	11232-11234	VBZ	denotes	is
T2001	11235-11245	JJ	denotes	unaffected
T2002	11246-11248	IN	denotes	by
T2003	11249-11252	DT	denotes	the
T2004	11253-11261	NN	denotes	deletion
T2005	11262-11264	IN	denotes	of
T2006	11265-11269	NN	denotes	exon
T2007	11270-11272	CD	denotes	18
T2008	11273-11274	-LRB-	denotes	(
T2010	11274-11280	NN	denotes	Figure
T2009	11281-11283	NN	denotes	2E
T2011	11283-11284	-RRB-	denotes	)
T2012	11284-11285	.	denotes	.
T2013	11285-11489	sentence	denotes	While the function of Atrxt is not yet clear, this isoform, which contains the PHD-like domain but not the SWI/SNF motifs (Figure 1), is unlikely to be functionally equivalent to the full-length protein.
T2014	11286-11291	IN	denotes	While
T2016	11292-11295	DT	denotes	the
T2017	11296-11304	NN	denotes	function
T2018	11305-11307	IN	denotes	of
T2019	11308-11313	NN	denotes	Atrxt
T2015	11314-11316	VBZ	denotes	is
T2021	11317-11320	RB	denotes	not
T2022	11321-11324	RB	denotes	yet
T2023	11325-11330	JJ	denotes	clear
T2024	11330-11332	,	denotes	,
T2025	11332-11336	DT	denotes	this
T2026	11337-11344	NN	denotes	isoform
T2027	11344-11346	,	denotes	,
T2028	11346-11351	WDT	denotes	which
T2029	11352-11360	VBZ	denotes	contains
T2030	11361-11364	DT	denotes	the
T2032	11365-11368	NN	denotes	PHD
T2034	11368-11369	HYPH	denotes	-
T2033	11369-11373	JJ	denotes	like
T2031	11374-11380	NN	denotes	domain
T2035	11381-11384	CC	denotes	but
T2036	11385-11388	RB	denotes	not
T2037	11389-11392	DT	denotes	the
T2039	11393-11396	NN	denotes	SWI
T2041	11396-11397	HYPH	denotes	/
T2040	11397-11400	NN	denotes	SNF
T2038	11401-11407	NNS	denotes	motifs
T2042	11408-11409	-LRB-	denotes	(
T2043	11409-11415	NN	denotes	Figure
T2044	11416-11417	CD	denotes	1
T2045	11417-11418	-RRB-	denotes	)
T2046	11418-11420	,	denotes	,
T2020	11420-11422	VBZ	denotes	is
T2047	11423-11431	JJ	denotes	unlikely
T2048	11432-11434	TO	denotes	to
T2049	11435-11437	VB	denotes	be
T2050	11438-11450	RB	denotes	functionally
T2051	11451-11461	JJ	denotes	equivalent
T2052	11462-11464	IN	denotes	to
T2053	11465-11468	DT	denotes	the
T2055	11469-11473	JJ	denotes	full
T2057	11473-11474	HYPH	denotes	-
T2056	11474-11480	NN	denotes	length
T2054	11481-11488	NN	denotes	protein
T2058	11488-11489	.	denotes	.
T2059	11489-11597	sentence	denotes	Thus, a conditional knockout strategy allowed the isolation of ES cells that are null for full-length Atrx.
T2060	11490-11494	RB	denotes	Thus
T2062	11494-11496	,	denotes	,
T2063	11496-11497	DT	denotes	a
T2065	11498-11509	JJ	denotes	conditional
T2066	11510-11518	NN	denotes	knockout
T2064	11519-11527	NN	denotes	strategy
T2061	11528-11535	VBD	denotes	allowed
T2067	11536-11539	DT	denotes	the
T2068	11540-11549	NN	denotes	isolation
T2069	11550-11552	IN	denotes	of
T2070	11553-11555	NN	denotes	ES
T2071	11556-11561	NNS	denotes	cells
T2072	11562-11566	WDT	denotes	that
T2073	11567-11570	VBP	denotes	are
T2074	11571-11575	JJ	denotes	null
T2075	11576-11579	IN	denotes	for
T2076	11580-11584	JJ	denotes	full
T2078	11584-11585	HYPH	denotes	-
T2077	11585-11591	NN	denotes	length
T2079	11592-11596	NN	denotes	Atrx
T2080	11596-11597	.	denotes	.
T2534	11599-11608	VBN	denotes	Perturbed
T2535	11609-11615	NN	denotes	Growth
T2536	11616-11619	CC	denotes	and
T2537	11620-11631	NN	denotes	Methylation
T2538	11632-11639	NNS	denotes	Defects
T2539	11640-11642	IN	denotes	in
T2540	11643-11651	JJ	denotes	Atrxnull
T2542	11652-11654	NN	denotes	ES
T2541	11655-11660	NNS	denotes	Cells
T2543	11660-11834	sentence	denotes	Atrxnull ES cells could be maintained in culture but were generally slower growing than Atrx+ ES clones, and appeared to undergo higher rates of spontaneous differentiation.
T2544	11661-11669	JJ	denotes	Atrxnull
T2546	11670-11672	NN	denotes	ES
T2545	11673-11678	NNS	denotes	cells
T2548	11679-11684	MD	denotes	could
T2549	11685-11687	VB	denotes	be
T2547	11688-11698	VBN	denotes	maintained
T2550	11699-11701	IN	denotes	in
T2551	11702-11709	NN	denotes	culture
T2552	11710-11713	CC	denotes	but
T2553	11714-11718	VBD	denotes	were
T2554	11719-11728	RB	denotes	generally
T2555	11729-11735	RBR	denotes	slower
T2556	11736-11743	VBG	denotes	growing
T2557	11744-11748	IN	denotes	than
T2558	11749-11753	NN	denotes	Atrx
T2560	11753-11754	SYM	denotes	+
T2559	11755-11757	NN	denotes	ES
T2561	11758-11764	NNS	denotes	clones
T2562	11764-11766	,	denotes	,
T2563	11766-11769	CC	denotes	and
T2564	11770-11778	VBD	denotes	appeared
T2565	11779-11781	TO	denotes	to
T2566	11782-11789	VB	denotes	undergo
T2567	11790-11796	JJR	denotes	higher
T2568	11797-11802	NNS	denotes	rates
T2569	11803-11805	IN	denotes	of
T2570	11806-11817	JJ	denotes	spontaneous
T2571	11818-11833	NN	denotes	differentiation
T2572	11833-11834	.	denotes	.
T2573	11834-11968	sentence	denotes	We investigated directly the effect of Atrx on ES cell growth by comparing Atrx+ and Atrxnull ES cell clones in competition cultures.
T2574	11835-11837	PRP	denotes	We
T2575	11838-11850	VBD	denotes	investigated
T2576	11851-11859	RB	denotes	directly
T2577	11860-11863	DT	denotes	the
T2578	11864-11870	NN	denotes	effect
T2579	11871-11873	IN	denotes	of
T2580	11874-11878	NN	denotes	Atrx
T2581	11879-11881	IN	denotes	on
T2582	11882-11884	NN	denotes	ES
T2583	11885-11889	NN	denotes	cell
T2584	11890-11896	NN	denotes	growth
T2585	11897-11899	IN	denotes	by
T2586	11900-11909	VBG	denotes	comparing
T2587	11910-11914	NN	denotes	Atrx
T2589	11914-11915	SYM	denotes	+
T2590	11916-11919	CC	denotes	and
T2591	11920-11928	JJ	denotes	Atrxnull
T2592	11929-11931	NN	denotes	ES
T2593	11932-11936	NN	denotes	cell
T2588	11937-11943	NNS	denotes	clones
T2594	11944-11946	IN	denotes	in
T2595	11947-11958	NN	denotes	competition
T2596	11959-11967	NNS	denotes	cultures
T2597	11967-11968	.	denotes	.
T2598	11968-12198	sentence	denotes	Equal numbers of Atrx+ (bearing either an Atrx WT or an Atrx flox allele) and Atrxnull (bearing an Atrx Δ18Δneo allele) ES cells were inoculated into cultures and the mixed cultures were passaged (1:3 split) every 2 d for 8–10 d.
T2599	11969-11974	JJ	denotes	Equal
T2600	11975-11982	NNS	denotes	numbers
T2602	11983-11985	IN	denotes	of
T2603	11986-11990	NN	denotes	Atrx
T2605	11990-11991	SYM	denotes	+
T2606	11992-11993	-LRB-	denotes	(
T2607	11993-12000	VBG	denotes	bearing
T2608	12001-12007	CC	denotes	either
T2610	12008-12010	DT	denotes	an
T2609	12011-12018	NN	denotes	Atrx WT
T2611	12019-12021	CC	denotes	or
T2612	12022-12024	DT	denotes	an
T2613	12025-12034	NN	denotes	Atrx flox
T2614	12035-12041	NN	denotes	allele
T2615	12041-12042	-RRB-	denotes	)
T2616	12043-12046	CC	denotes	and
T2617	12047-12055	JJ	denotes	Atrxnull
T2618	12056-12057	-LRB-	denotes	(
T2619	12057-12064	VBG	denotes	bearing
T2620	12065-12067	DT	denotes	an
T2622	12068-12080	NN	denotes	Atrx Δ18Δneo
T2621	12081-12087	NN	denotes	allele
T2623	12087-12088	-RRB-	denotes	)
T2624	12089-12091	NN	denotes	ES
T2604	12092-12097	NNS	denotes	cells
T2625	12098-12102	VBD	denotes	were
T2601	12103-12113	VBN	denotes	inoculated
T2626	12114-12118	IN	denotes	into
T2627	12119-12127	NNS	denotes	cultures
T2628	12128-12131	CC	denotes	and
T2629	12132-12135	DT	denotes	the
T2631	12136-12141	VBN	denotes	mixed
T2630	12142-12150	NNS	denotes	cultures
T2633	12151-12155	VBD	denotes	were
T2632	12156-12164	VBN	denotes	passaged
T2634	12165-12166	-LRB-	denotes	(
T2636	12166-12167	CD	denotes	1
T2637	12167-12168	SYM	denotes	:
T2638	12168-12169	CD	denotes	3
T2635	12170-12175	NN	denotes	split
T2639	12175-12176	-RRB-	denotes	)
T2640	12177-12182	DT	denotes	every
T2642	12183-12184	CD	denotes	2
T2641	12185-12186	NNS	denotes	d
T2643	12187-12190	IN	denotes	for
T2644	12191-12192	CD	denotes	8
T2646	12192-12193	SYM	denotes	–
T2645	12193-12195	CD	denotes	10
T2647	12196-12197	NNS	denotes	d
T2648	12197-12198	.	denotes	.
T2649	12198-12327	sentence	denotes	The relative abundance of the different alleles in the culture at each time point was analysed by Southern blotting (Figure 3A).
T2650	12199-12202	DT	denotes	The
T2652	12203-12211	JJ	denotes	relative
T2651	12212-12221	NN	denotes	abundance
T2654	12222-12224	IN	denotes	of
T2655	12225-12228	DT	denotes	the
T2657	12229-12238	JJ	denotes	different
T2656	12239-12246	NNS	denotes	alleles
T2658	12247-12249	IN	denotes	in
T2659	12250-12253	DT	denotes	the
T2660	12254-12261	NN	denotes	culture
T2661	12262-12264	IN	denotes	at
T2662	12265-12269	DT	denotes	each
T2664	12270-12274	NN	denotes	time
T2663	12275-12280	NN	denotes	point
T2665	12281-12284	VBD	denotes	was
T2653	12285-12293	VBN	denotes	analysed
T2666	12294-12296	IN	denotes	by
T2667	12297-12305	NNP	denotes	Southern
T2668	12306-12314	NN	denotes	blotting
T2669	12315-12316	-LRB-	denotes	(
T2671	12316-12322	NN	denotes	Figure
T2670	12323-12325	NN	denotes	3A
T2672	12325-12326	-RRB-	denotes	)
T2673	12326-12327	.	denotes	.
T2674	12327-12458	sentence	denotes	The clone containing the Atrx Δ18Δneo allele was rapidly outgrown by both Atrx WT ES cells and cells bearing the Atrx flox allele.
T2675	12328-12331	DT	denotes	The
T2676	12332-12337	NN	denotes	clone
T2678	12338-12348	VBG	denotes	containing
T2679	12349-12352	DT	denotes	the
T2681	12353-12365	NN	denotes	Atrx Δ18Δneo
T2680	12366-12372	NN	denotes	allele
T2682	12373-12376	VBD	denotes	was
T2683	12377-12384	RB	denotes	rapidly
T2677	12385-12393	VBN	denotes	outgrown
T2684	12394-12396	IN	denotes	by
T2685	12397-12401	CC	denotes	both
T2687	12402-12409	NN	denotes	Atrx WT
T2688	12410-12412	NN	denotes	ES
T2686	12413-12418	NNS	denotes	cells
T2689	12419-12422	CC	denotes	and
T2690	12423-12428	NNS	denotes	cells
T2691	12429-12436	VBG	denotes	bearing
T2692	12437-12440	DT	denotes	the
T2694	12441-12450	NN	denotes	Atrx flox
T2693	12451-12457	NN	denotes	allele
T2695	12457-12458	.	denotes	.
T2696	12458-12652	sentence	denotes	In a control competition experiment between different clones bearing functional Atrx alleles (Atrx WT and Atrx flox), both clones continued to be equally represented after 8 d of cocultivation.
T2697	12459-12461	IN	denotes	In
T2699	12462-12463	DT	denotes	a
T2701	12464-12471	NN	denotes	control
T2702	12472-12483	NN	denotes	competition
T2700	12484-12494	NN	denotes	experiment
T2703	12495-12502	IN	denotes	between
T2704	12503-12512	JJ	denotes	different
T2705	12513-12519	NNS	denotes	clones
T2706	12520-12527	VBG	denotes	bearing
T2707	12528-12538	JJ	denotes	functional
T2709	12539-12543	NN	denotes	Atrx
T2708	12544-12551	NNS	denotes	alleles
T2710	12552-12553	-LRB-	denotes	(
T2711	12553-12560	NN	denotes	Atrx WT
T2712	12561-12564	CC	denotes	and
T2713	12565-12574	NN	denotes	Atrx flox
T2714	12574-12575	-RRB-	denotes	)
T2715	12575-12577	,	denotes	,
T2716	12577-12581	DT	denotes	both
T2717	12582-12588	NNS	denotes	clones
T2698	12589-12598	VBD	denotes	continued
T2718	12599-12601	TO	denotes	to
T2719	12602-12604	VB	denotes	be
T2720	12605-12612	RB	denotes	equally
T2721	12613-12624	JJ	denotes	represented
T2722	12625-12630	IN	denotes	after
T2723	12631-12632	CD	denotes	8
T2724	12633-12634	NNS	denotes	d
T2725	12635-12637	IN	denotes	of
T2726	12638-12651	NN	denotes	cocultivation
T2727	12651-12652	.	denotes	.
T2728	12652-12885	sentence	denotes	Thus, although Atrxnull ES cells could be recovered and maintained in culture by a conditional targeting approach, these cocultivation experiments suggested that the absence of Atrx does negatively impact upon normal ES cell growth.
T2729	12653-12657	RB	denotes	Thus
T2731	12657-12659	,	denotes	,
T2732	12659-12667	IN	denotes	although
T2734	12668-12676	JJ	denotes	Atrxnull
T2736	12677-12679	NN	denotes	ES
T2735	12680-12685	NNS	denotes	cells
T2737	12686-12691	MD	denotes	could
T2738	12692-12694	VB	denotes	be
T2733	12695-12704	VBN	denotes	recovered
T2739	12705-12708	CC	denotes	and
T2740	12709-12719	VBN	denotes	maintained
T2741	12720-12722	IN	denotes	in
T2742	12723-12730	NN	denotes	culture
T2743	12731-12733	IN	denotes	by
T2744	12734-12735	DT	denotes	a
T2746	12736-12747	JJ	denotes	conditional
T2747	12748-12757	NN	denotes	targeting
T2745	12758-12766	NN	denotes	approach
T2748	12766-12768	,	denotes	,
T2749	12768-12773	DT	denotes	these
T2751	12774-12787	NN	denotes	cocultivation
T2750	12788-12799	NNS	denotes	experiments
T2730	12800-12809	VBD	denotes	suggested
T2752	12810-12814	IN	denotes	that
T2754	12815-12818	DT	denotes	the
T2755	12819-12826	NN	denotes	absence
T2756	12827-12829	IN	denotes	of
T2757	12830-12834	NN	denotes	Atrx
T2758	12835-12839	VBZ	denotes	does
T2759	12840-12850	RB	denotes	negatively
T2753	12851-12857	VB	denotes	impact
T2760	12858-12862	IN	denotes	upon
T2761	12863-12869	JJ	denotes	normal
T2763	12870-12872	NN	denotes	ES
T2764	12873-12877	NN	denotes	cell
T2762	12878-12884	NN	denotes	growth
T2765	12884-12885	.	denotes	.
T2766	12885-14751	sentence	denotes	Figure 3 Growth and Methylation Defects in Atrxnull ES Cells (A) Cultures were inoculated with equivalent numbers of ES cells bearing different Atrx alleles as indicated, and were serially passaged. After the indicated days of coculture, DNA extracted from a sample of cells was analysed by Southern blot to detect the Atrx alleles. DNA was digested with SpeI, and the membrane was hybridised with the 20/27 probe shown in Figure 2A. The expected sizes of the different alleles are indicated. (B) Schematic diagram of the transcribed portion of the mouse rDNA repeat with the 18S, 5.8S, and 28S genes indicated. The positions of the limit-digesting enzymes BamH (labelled B) and EcoRI (labelled E) and the probes (RIB3 and RIB4) used in the Southern blots shown in (C) are indicated. Below are shown the locations of the methylation-sensitive enzymes (SmaI, PvuI, and MluI) whose methylation status has been analysed in the Southern blots shown in (C). (C) DNA from Atrx-positive (Atrx+, bearing either an Atrx WT or Atrx flox allele) or Atrxnull (bearing the Atrx Δ18Δneo allele) ES cells and 7-d embryoid bodies were digested with the enzymes shown and analysed by Southern blotting using the probes indicated. Arrows indicate the fully methylated copies (cut by only the limit-digesting enzyme). Phosphorimager quantitation of the blots are shown below. The y-axis shows the percentage of copies that are undigested by the methylation-sensitive enzyme as a percentage of the total signal from cut and uncut rDNA. Mean values are indicated by horizontal lines, and the significance of the differences between the Atrx-positive and Atrxnull populations are shown for each enzyme. To investigate a possible cell-cycle defect in the absence of Atrx, we analysed the cell cycle distribution of bromodeoxyuridine (BrdU)-pulsed ES cells by flow cytometry (Figure S1A).
T2767	14568-14570	TO	denotes	To
T2768	14571-14582	VB	denotes	investigate
T2770	14583-14584	DT	denotes	a
T2772	14585-14593	JJ	denotes	possible
T2773	14594-14598	NN	denotes	cell
T2775	14598-14599	HYPH	denotes	-
T2774	14599-14604	NN	denotes	cycle
T2771	14605-14611	NN	denotes	defect
T2776	14612-14614	IN	denotes	in
T2777	14615-14618	DT	denotes	the
T2778	14619-14626	NN	denotes	absence
T2779	14627-14629	IN	denotes	of
T2780	14630-14634	NN	denotes	Atrx
T2781	14634-14636	,	denotes	,
T2782	14636-14638	PRP	denotes	we
T2769	14639-14647	VBD	denotes	analysed
T2783	14648-14651	DT	denotes	the
T2785	14652-14656	NN	denotes	cell
T2786	14657-14662	NN	denotes	cycle
T2784	14663-14675	NN	denotes	distribution
T2787	14676-14678	IN	denotes	of
T2788	14679-14696	NN	denotes	bromodeoxyuridine
T2790	14697-14698	-LRB-	denotes	(
T2791	14698-14702	NN	denotes	BrdU
T2792	14702-14703	-RRB-	denotes	)
T2793	14703-14704	HYPH	denotes	-
T2789	14704-14710	VBN	denotes	pulsed
T2795	14711-14713	NN	denotes	ES
T2794	14714-14719	NNS	denotes	cells
T2796	14720-14722	IN	denotes	by
T2797	14723-14727	NN	denotes	flow
T2798	14728-14737	NN	denotes	cytometry
T2799	14738-14739	-LRB-	denotes	(
T2801	14739-14745	NN	denotes	Figure
T2800	14746-14749	NN	denotes	S1A
T2802	14749-14750	-RRB-	denotes	)
T2803	14750-14751	.	denotes	.
T2804	14751-14923	sentence	denotes	Surprisingly, both Atrxnull ES cell clones exhibited a cell cycle profile that was indistinguishable from ES cells bearing a functional Atrx allele (Atrx WT or Atrx flox).
T2805	14752-14764	RB	denotes	Surprisingly
T2807	14764-14766	,	denotes	,
T2808	14766-14770	DT	denotes	both
T2810	14771-14779	JJ	denotes	Atrxnull
T2812	14780-14782	NN	denotes	ES
T2811	14783-14787	NN	denotes	cell
T2809	14788-14794	NNS	denotes	clones
T2806	14795-14804	VBD	denotes	exhibited
T2813	14805-14806	DT	denotes	a
T2815	14807-14811	NN	denotes	cell
T2816	14812-14817	NN	denotes	cycle
T2814	14818-14825	NN	denotes	profile
T2817	14826-14830	WDT	denotes	that
T2818	14831-14834	VBD	denotes	was
T2819	14835-14852	JJ	denotes	indistinguishable
T2820	14853-14857	IN	denotes	from
T2821	14858-14860	NN	denotes	ES
T2822	14861-14866	NNS	denotes	cells
T2823	14867-14874	VBG	denotes	bearing
T2824	14875-14876	DT	denotes	a
T2826	14877-14887	JJ	denotes	functional
T2827	14888-14892	NN	denotes	Atrx
T2825	14893-14899	NN	denotes	allele
T2828	14900-14901	-LRB-	denotes	(
T2829	14901-14908	NN	denotes	Atrx WT
T2830	14909-14911	CC	denotes	or
T2831	14912-14921	NN	denotes	Atrx flox
T2832	14921-14922	-RRB-	denotes	)
T2833	14922-14923	.	denotes	.
T2834	14923-15126	sentence	denotes	We also specifically quantitated the mitotic index within each population by flow cytometry after staining ES cells for phosphorylated (Ser10) histone H3, a specific marker of mitosis (Figure S1B) [13].
T2835	14924-14926	PRP	denotes	We
T2837	14927-14931	RB	denotes	also
T2838	14932-14944	RB	denotes	specifically
T2836	14945-14956	VBD	denotes	quantitated
T2839	14957-14960	DT	denotes	the
T2841	14961-14968	JJ	denotes	mitotic
T2840	14969-14974	NN	denotes	index
T2842	14975-14981	IN	denotes	within
T2843	14982-14986	DT	denotes	each
T2844	14987-14997	NN	denotes	population
T2845	14998-15000	IN	denotes	by
T2846	15001-15005	NN	denotes	flow
T2847	15006-15015	NN	denotes	cytometry
T2848	15016-15021	IN	denotes	after
T2849	15022-15030	VBG	denotes	staining
T2850	15031-15033	NN	denotes	ES
T2851	15034-15039	NNS	denotes	cells
T2852	15040-15043	IN	denotes	for
T2853	15044-15058	VBN	denotes	phosphorylated
T2855	15059-15060	-LRB-	denotes	(
T2856	15060-15065	NN	denotes	Ser10
T2857	15065-15066	-RRB-	denotes	)
T2858	15067-15074	NN	denotes	histone
T2854	15075-15077	NN	denotes	H3
T2859	15077-15079	,	denotes	,
T2860	15079-15080	DT	denotes	a
T2862	15081-15089	JJ	denotes	specific
T2861	15090-15096	NN	denotes	marker
T2863	15097-15099	IN	denotes	of
T2864	15100-15107	NN	denotes	mitosis
T2865	15108-15109	-LRB-	denotes	(
T2867	15109-15115	NN	denotes	Figure
T2866	15116-15119	NN	denotes	S1B
T2868	15119-15120	-RRB-	denotes	)
T2869	15121-15122	-LRB-	denotes	[
T2870	15122-15124	CD	denotes	13
T2871	15124-15125	-RRB-	denotes	]
T2872	15125-15126	.	denotes	.
T2873	15126-15303	sentence	denotes	Consistent with the normal cell-cycle profile observed above, there was no depletion in the size of the mitotic population in the Atrxnull ES clones, despite their slow growth.
T2874	15127-15137	JJ	denotes	Consistent
T2876	15138-15142	IN	denotes	with
T2877	15143-15146	DT	denotes	the
T2879	15147-15153	JJ	denotes	normal
T2880	15154-15158	NN	denotes	cell
T2882	15158-15159	HYPH	denotes	-
T2881	15159-15164	NN	denotes	cycle
T2878	15165-15172	NN	denotes	profile
T2883	15173-15181	VBN	denotes	observed
T2884	15182-15187	RB	denotes	above
T2885	15187-15189	,	denotes	,
T2886	15189-15194	EX	denotes	there
T2875	15195-15198	VBD	denotes	was
T2887	15199-15201	DT	denotes	no
T2888	15202-15211	NN	denotes	depletion
T2889	15212-15214	IN	denotes	in
T2890	15215-15218	DT	denotes	the
T2891	15219-15223	NN	denotes	size
T2892	15224-15226	IN	denotes	of
T2893	15227-15230	DT	denotes	the
T2895	15231-15238	JJ	denotes	mitotic
T2894	15239-15249	NN	denotes	population
T2896	15250-15252	IN	denotes	in
T2897	15253-15256	DT	denotes	the
T2899	15257-15265	JJ	denotes	Atrxnull
T2900	15266-15268	NN	denotes	ES
T2898	15269-15275	NNS	denotes	clones
T2901	15275-15277	,	denotes	,
T2902	15277-15284	IN	denotes	despite
T2903	15285-15290	PRP$	denotes	their
T2905	15291-15295	JJ	denotes	slow
T2904	15296-15302	NN	denotes	growth
T2906	15302-15303	.	denotes	.
T2907	15303-15581	sentence	denotes	Finally, we investigated whether the growth defect in the Atrxnull ES cells was due to an up-regulation of apoptosis by staining cells with Annexin V (Figure S2) and found that the proportion of apoptotic cells was not significantly affected by the absence of full-length Atrx.
T2908	15304-15311	RB	denotes	Finally
T2910	15311-15313	,	denotes	,
T2911	15313-15315	PRP	denotes	we
T2909	15316-15328	VBD	denotes	investigated
T2912	15329-15336	IN	denotes	whether
T2914	15337-15340	DT	denotes	the
T2916	15341-15347	NN	denotes	growth
T2915	15348-15354	NN	denotes	defect
T2917	15355-15357	IN	denotes	in
T2918	15358-15361	DT	denotes	the
T2920	15362-15370	JJ	denotes	Atrxnull
T2921	15371-15373	NN	denotes	ES
T2919	15374-15379	NNS	denotes	cells
T2913	15380-15383	VBD	denotes	was
T2922	15384-15387	IN	denotes	due
T2923	15388-15390	IN	denotes	to
T2924	15391-15393	DT	denotes	an
T2926	15394-15396	JJ	denotes	up
T2927	15396-15397	HYPH	denotes	-
T2925	15397-15407	NN	denotes	regulation
T2928	15408-15410	IN	denotes	of
T2929	15411-15420	NN	denotes	apoptosis
T2930	15421-15423	IN	denotes	by
T2931	15424-15432	VBG	denotes	staining
T2932	15433-15438	NNS	denotes	cells
T2933	15439-15443	IN	denotes	with
T2934	15444-15451	NN	denotes	Annexin
T2935	15452-15453	CD	denotes	V
T2936	15454-15455	-LRB-	denotes	(
T2938	15455-15461	NN	denotes	Figure
T2937	15462-15464	NN	denotes	S2
T2939	15464-15465	-RRB-	denotes	)
T2940	15466-15469	CC	denotes	and
T2941	15470-15475	VBD	denotes	found
T2942	15476-15480	IN	denotes	that
T2944	15481-15484	DT	denotes	the
T2945	15485-15495	NN	denotes	proportion
T2946	15496-15498	IN	denotes	of
T2947	15499-15508	JJ	denotes	apoptotic
T2948	15509-15514	NNS	denotes	cells
T2949	15515-15518	VBD	denotes	was
T2950	15519-15522	RB	denotes	not
T2951	15523-15536	RB	denotes	significantly
T2943	15537-15545	VBN	denotes	affected
T2952	15546-15548	IN	denotes	by
T2953	15549-15552	DT	denotes	the
T2954	15553-15560	NN	denotes	absence
T2955	15561-15563	IN	denotes	of
T2956	15564-15568	JJ	denotes	full
T2958	15568-15569	HYPH	denotes	-
T2957	15569-15575	NN	denotes	length
T2959	15576-15580	NN	denotes	Atrx
T2960	15580-15581	.	denotes	.
T2961	15581-15721	sentence	denotes	Thus, the growth defect observed in ES cells lacking Atrx is not due to a specific cell cycle block or significant induction of cell death.
T2962	15582-15586	RB	denotes	Thus
T2964	15586-15588	,	denotes	,
T2965	15588-15591	DT	denotes	the
T2967	15592-15598	NN	denotes	growth
T2966	15599-15605	NN	denotes	defect
T2968	15606-15614	VBN	denotes	observed
T2969	15615-15617	IN	denotes	in
T2970	15618-15620	NN	denotes	ES
T2971	15621-15626	NNS	denotes	cells
T2972	15627-15634	VBG	denotes	lacking
T2973	15635-15639	NN	denotes	Atrx
T2963	15640-15642	VBZ	denotes	is
T2974	15643-15646	RB	denotes	not
T2975	15647-15650	IN	denotes	due
T2976	15651-15653	IN	denotes	to
T2977	15654-15655	DT	denotes	a
T2979	15656-15664	JJ	denotes	specific
T2980	15665-15669	NN	denotes	cell
T2981	15670-15675	NN	denotes	cycle
T2978	15676-15681	NN	denotes	block
T2982	15682-15684	CC	denotes	or
T2983	15685-15696	JJ	denotes	significant
T2984	15697-15706	NN	denotes	induction
T2985	15707-15709	IN	denotes	of
T2986	15710-15714	NN	denotes	cell
T2987	15715-15720	NN	denotes	death
T2988	15720-15721	.	denotes	.
T2989	15721-16088	sentence	denotes	While the cause of the proliferative delay is not yet clear, since Atrxnull ES cells appear to undergo higher rates of spontaneous differentiation (unpublished data), it seems likely that the observed growth defect reflects the spontaneous transition from fast-cycling, undifferentiated ES cells into more slowly cycling, differentiated cell types in these cultures.
T2990	15722-15727	IN	denotes	While
T2992	15728-15731	DT	denotes	the
T2993	15732-15737	NN	denotes	cause
T2994	15738-15740	IN	denotes	of
T2995	15741-15744	DT	denotes	the
T2997	15745-15758	JJ	denotes	proliferative
T2996	15759-15764	NN	denotes	delay
T2991	15765-15767	VBZ	denotes	is
T2999	15768-15771	RB	denotes	not
T3000	15772-15775	RB	denotes	yet
T3001	15776-15781	JJ	denotes	clear
T3002	15781-15783	,	denotes	,
T3003	15783-15788	IN	denotes	since
T3005	15789-15797	JJ	denotes	Atrxnull
T3007	15798-15800	NN	denotes	ES
T3006	15801-15806	NNS	denotes	cells
T3004	15807-15813	VBP	denotes	appear
T3008	15814-15816	TO	denotes	to
T3009	15817-15824	VB	denotes	undergo
T3010	15825-15831	JJR	denotes	higher
T3011	15832-15837	NNS	denotes	rates
T3012	15838-15840	IN	denotes	of
T3013	15841-15852	JJ	denotes	spontaneous
T3014	15853-15868	NN	denotes	differentiation
T3015	15869-15870	-LRB-	denotes	(
T3017	15870-15881	JJ	denotes	unpublished
T3016	15882-15886	NNS	denotes	data
T3018	15886-15887	-RRB-	denotes	)
T3019	15887-15889	,	denotes	,
T3020	15889-15891	PRP	denotes	it
T2998	15892-15897	VBZ	denotes	seems
T3021	15898-15904	JJ	denotes	likely
T3022	15905-15909	IN	denotes	that
T3024	15910-15913	DT	denotes	the
T3026	15914-15922	VBN	denotes	observed
T3027	15923-15929	NN	denotes	growth
T3025	15930-15936	NN	denotes	defect
T3023	15937-15945	VBZ	denotes	reflects
T3028	15946-15949	DT	denotes	the
T3030	15950-15961	JJ	denotes	spontaneous
T3029	15962-15972	NN	denotes	transition
T3031	15973-15977	IN	denotes	from
T3032	15978-15982	RB	denotes	fast
T3034	15982-15983	HYPH	denotes	-
T3033	15983-15990	VBG	denotes	cycling
T3036	15990-15992	,	denotes	,
T3037	15992-16008	JJ	denotes	undifferentiated
T3038	16009-16011	NN	denotes	ES
T3035	16012-16017	NNS	denotes	cells
T3039	16018-16022	IN	denotes	into
T3040	16023-16027	RBR	denotes	more
T3041	16028-16034	RB	denotes	slowly
T3042	16035-16042	VBG	denotes	cycling
T3044	16042-16044	,	denotes	,
T3045	16044-16058	JJ	denotes	differentiated
T3046	16059-16063	NN	denotes	cell
T3043	16064-16069	NNS	denotes	types
T3047	16070-16072	IN	denotes	in
T3048	16073-16078	DT	denotes	these
T3049	16079-16087	NNS	denotes	cultures
T3050	16087-16088	.	denotes	.
T3051	16088-16284	sentence	denotes	It has been shown that disease-causing mutations in the human ATRX gene give rise to changes in the normal pattern of DNA methylation at several repetitive sequences within the human genome [11].
T3052	16089-16091	PRP	denotes	It
T3054	16092-16095	VBZ	denotes	has
T3055	16096-16100	VBN	denotes	been
T3053	16101-16106	VBN	denotes	shown
T3056	16107-16111	IN	denotes	that
T3058	16112-16119	NN	denotes	disease
T3060	16119-16120	HYPH	denotes	-
T3059	16120-16127	VBG	denotes	causing
T3061	16128-16137	NNS	denotes	mutations
T3062	16138-16140	IN	denotes	in
T3063	16141-16144	DT	denotes	the
T3065	16145-16150	JJ	denotes	human
T3066	16151-16155	NN	denotes	ATRX
T3064	16156-16160	NN	denotes	gene
T3057	16161-16165	VBP	denotes	give
T3067	16166-16170	NN	denotes	rise
T3068	16171-16173	IN	denotes	to
T3069	16174-16181	NNS	denotes	changes
T3070	16182-16184	IN	denotes	in
T3071	16185-16188	DT	denotes	the
T3073	16189-16195	JJ	denotes	normal
T3072	16196-16203	NN	denotes	pattern
T3074	16204-16206	IN	denotes	of
T3075	16207-16210	NN	denotes	DNA
T3076	16211-16222	NN	denotes	methylation
T3077	16223-16225	IN	denotes	at
T3078	16226-16233	JJ	denotes	several
T3080	16234-16244	JJ	denotes	repetitive
T3079	16245-16254	NNS	denotes	sequences
T3081	16255-16261	IN	denotes	within
T3082	16262-16265	DT	denotes	the
T3084	16266-16271	JJ	denotes	human
T3083	16272-16278	NN	denotes	genome
T3085	16279-16280	-LRB-	denotes	[
T3086	16280-16282	CD	denotes	11
T3087	16282-16283	-RRB-	denotes	]
T3088	16283-16284	.	denotes	.
T3089	16284-16446	sentence	denotes	Notably, the transcribed region of the ribosomal DNA (rDNA) repeat was found to be significantly hypomethylated in ATR-X patients relative to normal individuals.
T3090	16285-16292	RB	denotes	Notably
T3092	16292-16294	,	denotes	,
T3093	16294-16297	DT	denotes	the
T3095	16298-16309	VBN	denotes	transcribed
T3094	16310-16316	NN	denotes	region
T3096	16317-16319	IN	denotes	of
T3097	16320-16323	DT	denotes	the
T3099	16324-16333	JJ	denotes	ribosomal
T3100	16334-16337	NN	denotes	DNA
T3101	16338-16339	-LRB-	denotes	(
T3102	16339-16343	NN	denotes	rDNA
T3103	16343-16344	-RRB-	denotes	)
T3098	16345-16351	NN	denotes	repeat
T3104	16352-16355	VBD	denotes	was
T3091	16356-16361	VBN	denotes	found
T3105	16362-16364	TO	denotes	to
T3107	16365-16367	VB	denotes	be
T3108	16368-16381	RB	denotes	significantly
T3106	16382-16396	VBN	denotes	hypomethylated
T3109	16397-16399	IN	denotes	in
T3110	16400-16403	NN	denotes	ATR
T3112	16403-16404	HYPH	denotes	-
T3111	16404-16405	NN	denotes	X
T3113	16406-16414	NNS	denotes	patients
T3114	16415-16423	JJ	denotes	relative
T3115	16424-16426	IN	denotes	to
T3116	16427-16433	JJ	denotes	normal
T3117	16434-16445	NNS	denotes	individuals
T3118	16445-16446	.	denotes	.
T3119	16446-16757	sentence	denotes	Using methylation-sensitive restriction enzymes, we also observed significant hypomethylation at several sites tested within the mouse rDNA repeats in Atrxnull ES cells and 12-d embryoid bodies relative to ES cells and embryoid bodies bearing a functional Atrx allele (Atrx WT or Atrx flox) (Figure 3B and 3C).
T3120	16447-16452	VBG	denotes	Using
T3122	16453-16464	NN	denotes	methylation
T3124	16464-16465	HYPH	denotes	-
T3123	16465-16474	JJ	denotes	sensitive
T3126	16475-16486	NN	denotes	restriction
T3125	16487-16494	NNS	denotes	enzymes
T3127	16494-16496	,	denotes	,
T3128	16496-16498	PRP	denotes	we
T3129	16499-16503	RB	denotes	also
T3121	16504-16512	VBD	denotes	observed
T3130	16513-16524	JJ	denotes	significant
T3131	16525-16540	NN	denotes	hypomethylation
T3132	16541-16543	IN	denotes	at
T3133	16544-16551	JJ	denotes	several
T3134	16552-16557	NNS	denotes	sites
T3135	16558-16564	VBN	denotes	tested
T3136	16565-16571	IN	denotes	within
T3137	16572-16575	DT	denotes	the
T3139	16576-16581	NN	denotes	mouse
T3140	16582-16586	NN	denotes	rDNA
T3138	16587-16594	NNS	denotes	repeats
T3141	16595-16597	IN	denotes	in
T3142	16598-16606	JJ	denotes	Atrxnull
T3144	16607-16609	NN	denotes	ES
T3143	16610-16615	NNS	denotes	cells
T3145	16616-16619	CC	denotes	and
T3146	16620-16622	CD	denotes	12
T3148	16622-16623	HYPH	denotes	-
T3147	16623-16624	NN	denotes	d
T3150	16625-16633	JJ	denotes	embryoid
T3149	16634-16640	NNS	denotes	bodies
T3151	16641-16649	JJ	denotes	relative
T3152	16650-16652	IN	denotes	to
T3153	16653-16655	NN	denotes	ES
T3154	16656-16661	NNS	denotes	cells
T3155	16662-16665	CC	denotes	and
T3156	16666-16674	JJ	denotes	embryoid
T3157	16675-16681	NNS	denotes	bodies
T3158	16682-16689	VBG	denotes	bearing
T3159	16690-16691	DT	denotes	a
T3161	16692-16702	JJ	denotes	functional
T3162	16703-16707	NN	denotes	Atrx
T3160	16708-16714	NN	denotes	allele
T3163	16715-16716	-LRB-	denotes	(
T3164	16716-16723	NN	denotes	Atrx WT
T3165	16724-16726	CC	denotes	or
T3166	16727-16736	NN	denotes	Atrx flox
T3167	16736-16737	-RRB-	denotes	)
T3168	16738-16739	-LRB-	denotes	(
T3170	16739-16745	NN	denotes	Figure
T3169	16746-16748	NN	denotes	3B
T3171	16749-16752	CC	denotes	and
T3172	16753-16755	NN	denotes	3C
T3173	16755-16756	-RRB-	denotes	)
T3174	16756-16757	.	denotes	.
T3175	16757-16988	sentence	denotes	The observation that rDNA is hypomethylated in the absence of Atrx, even in ES cells, is consistent with the finding that hypomethylation of the human rDNA repeats is detectable from an early developmental stage in ATR-X patients.
T3176	16758-16761	DT	denotes	The
T3177	16762-16773	NN	denotes	observation
T3179	16774-16778	IN	denotes	that
T3181	16779-16783	NN	denotes	rDNA
T3182	16784-16786	VBZ	denotes	is
T3180	16787-16801	VBN	denotes	hypomethylated
T3183	16802-16804	IN	denotes	in
T3184	16805-16808	DT	denotes	the
T3185	16809-16816	NN	denotes	absence
T3186	16817-16819	IN	denotes	of
T3187	16820-16824	NN	denotes	Atrx
T3188	16824-16826	,	denotes	,
T3189	16826-16830	RB	denotes	even
T3190	16831-16833	IN	denotes	in
T3191	16834-16836	NN	denotes	ES
T3192	16837-16842	NNS	denotes	cells
T3193	16842-16844	,	denotes	,
T3178	16844-16846	VBZ	denotes	is
T3194	16847-16857	JJ	denotes	consistent
T3195	16858-16862	IN	denotes	with
T3196	16863-16866	DT	denotes	the
T3197	16867-16874	NN	denotes	finding
T3198	16875-16879	IN	denotes	that
T3200	16880-16895	NN	denotes	hypomethylation
T3201	16896-16898	IN	denotes	of
T3202	16899-16902	DT	denotes	the
T3204	16903-16908	JJ	denotes	human
T3205	16909-16913	NN	denotes	rDNA
T3203	16914-16921	NNS	denotes	repeats
T3199	16922-16924	VBZ	denotes	is
T3206	16925-16935	JJ	denotes	detectable
T3207	16936-16940	IN	denotes	from
T3208	16941-16943	DT	denotes	an
T3210	16944-16949	JJ	denotes	early
T3211	16950-16963	JJ	denotes	developmental
T3209	16964-16969	NN	denotes	stage
T3212	16970-16972	IN	denotes	in
T3213	16973-16976	NN	denotes	ATR
T3215	16976-16977	HYPH	denotes	-
T3214	16977-16978	NN	denotes	X
T3216	16979-16987	NNS	denotes	patients
T3217	16987-16988	.	denotes	.
T3218	16988-17317	sentence	denotes	Other mouse repetitive sequence elements surveyed in ES cell DNA include the heterochromatic major satellite (assayed with MaeII) and minor satellite (assayed with HpaII) repeats, as well as interspersed retroviral repeats of the intracisternal A particle (IAP) type and the Line 1 and Sine B1 families (all assayed with HpaII).
T3219	16989-16994	JJ	denotes	Other
T3221	16995-17000	NN	denotes	mouse
T3222	17001-17011	JJ	denotes	repetitive
T3223	17012-17020	NN	denotes	sequence
T3220	17021-17029	NNS	denotes	elements
T3225	17030-17038	VBN	denotes	surveyed
T3226	17039-17041	IN	denotes	in
T3227	17042-17044	NN	denotes	ES
T3229	17045-17049	NN	denotes	cell
T3228	17050-17053	NN	denotes	DNA
T3224	17054-17061	VBP	denotes	include
T3230	17062-17065	DT	denotes	the
T3232	17066-17081	JJ	denotes	heterochromatic
T3234	17082-17087	JJ	denotes	major
T3233	17088-17097	NN	denotes	satellite
T3235	17098-17099	-LRB-	denotes	(
T3236	17099-17106	VBN	denotes	assayed
T3237	17107-17111	IN	denotes	with
T3238	17112-17117	NN	denotes	MaeII
T3239	17117-17118	-RRB-	denotes	)
T3240	17119-17122	CC	denotes	and
T3241	17123-17128	JJ	denotes	minor
T3242	17129-17138	NN	denotes	satellite
T3243	17139-17140	-LRB-	denotes	(
T3244	17140-17147	VBN	denotes	assayed
T3245	17148-17152	IN	denotes	with
T3246	17153-17158	NN	denotes	HpaII
T3247	17158-17159	-RRB-	denotes	)
T3231	17160-17167	NNS	denotes	repeats
T3248	17167-17169	,	denotes	,
T3249	17169-17171	RB	denotes	as
T3251	17172-17176	RB	denotes	well
T3250	17177-17179	IN	denotes	as
T3252	17180-17192	VBN	denotes	interspersed
T3254	17193-17203	JJ	denotes	retroviral
T3253	17204-17211	NNS	denotes	repeats
T3255	17212-17214	IN	denotes	of
T3256	17215-17218	DT	denotes	the
T3258	17219-17233	JJ	denotes	intracisternal
T3260	17234-17235	NN	denotes	A
T3259	17236-17244	NN	denotes	particle
T3261	17245-17246	-LRB-	denotes	(
T3262	17246-17249	NN	denotes	IAP
T3263	17249-17250	-RRB-	denotes	)
T3257	17251-17255	NN	denotes	type
T3264	17256-17259	CC	denotes	and
T3265	17260-17263	DT	denotes	the
T3267	17264-17268	NN	denotes	Line
T3268	17269-17270	CD	denotes	1
T3269	17271-17274	CC	denotes	and
T3270	17275-17279	NN	denotes	Sine
T3271	17280-17282	NN	denotes	B1
T3266	17283-17291	NNS	denotes	families
T3272	17292-17293	-LRB-	denotes	(
T3273	17293-17296	DT	denotes	all
T3274	17297-17304	VBN	denotes	assayed
T3275	17305-17309	IN	denotes	with
T3276	17310-17315	NN	denotes	HpaII
T3277	17315-17316	-RRB-	denotes	)
T3278	17316-17317	.	denotes	.
T3279	17317-17571	sentence	denotes	These repeats were found to be moderately (Line 1 and Sine B1) or highly (IAP, major satellite, minor satellite) methylated in wild-type ES cells, and this methylation was not detectably perturbed by the absence of Atrx (Figure S3 and unpublished data).
T3280	17318-17323	DT	denotes	These
T3281	17324-17331	NNS	denotes	repeats
T3283	17332-17336	VBD	denotes	were
T3282	17337-17342	VBN	denotes	found
T3284	17343-17345	TO	denotes	to
T3285	17346-17348	VB	denotes	be
T3286	17349-17359	RB	denotes	moderately
T3288	17360-17361	-LRB-	denotes	(
T3289	17361-17365	NN	denotes	Line
T3290	17366-17367	CD	denotes	1
T3291	17368-17371	CC	denotes	and
T3292	17372-17376	NN	denotes	Sine
T3293	17377-17379	NN	denotes	B1
T3294	17379-17380	-RRB-	denotes	)
T3295	17381-17383	CC	denotes	or
T3296	17384-17390	RB	denotes	highly
T3297	17391-17392	-LRB-	denotes	(
T3298	17392-17395	NN	denotes	IAP
T3299	17395-17397	,	denotes	,
T3300	17397-17402	JJ	denotes	major
T3301	17403-17412	NN	denotes	satellite
T3302	17412-17414	,	denotes	,
T3303	17414-17419	JJ	denotes	minor
T3304	17420-17429	NN	denotes	satellite
T3305	17429-17430	-RRB-	denotes	)
T3287	17431-17441	JJ	denotes	methylated
T3306	17442-17444	IN	denotes	in
T3307	17445-17449	JJ	denotes	wild
T3309	17449-17450	HYPH	denotes	-
T3308	17450-17454	NN	denotes	type
T3311	17455-17457	NN	denotes	ES
T3310	17458-17463	NNS	denotes	cells
T3312	17463-17465	,	denotes	,
T3313	17465-17468	CC	denotes	and
T3314	17469-17473	DT	denotes	this
T3315	17474-17485	NN	denotes	methylation
T3317	17486-17489	VBD	denotes	was
T3318	17490-17493	RB	denotes	not
T3319	17494-17504	RB	denotes	detectably
T3316	17505-17514	VBN	denotes	perturbed
T3320	17515-17517	IN	denotes	by
T3321	17518-17521	DT	denotes	the
T3322	17522-17529	NN	denotes	absence
T3323	17530-17532	IN	denotes	of
T3324	17533-17537	NN	denotes	Atrx
T3325	17538-17539	-LRB-	denotes	(
T3327	17539-17545	NN	denotes	Figure
T3326	17546-17548	NN	denotes	S3
T3328	17549-17552	CC	denotes	and
T3329	17553-17564	JJ	denotes	unpublished
T3330	17565-17569	NNS	denotes	data
T3331	17569-17570	-RRB-	denotes	)
T3332	17570-17571	.	denotes	.
T3333	17571-17737	sentence	denotes	Taken together, these data indicate that the subtle interplay between the ATRX protein and DNA methylation observed in human patients is also present in mouse cells.
T3334	17572-17577	VBN	denotes	Taken
T3336	17578-17586	RB	denotes	together
T3337	17586-17588	,	denotes	,
T3338	17588-17593	DT	denotes	these
T3339	17594-17598	NNS	denotes	data
T3335	17599-17607	VBP	denotes	indicate
T3340	17608-17612	IN	denotes	that
T3342	17613-17616	DT	denotes	the
T3344	17617-17623	JJ	denotes	subtle
T3343	17624-17633	NN	denotes	interplay
T3345	17634-17641	IN	denotes	between
T3346	17642-17645	DT	denotes	the
T3348	17646-17650	NN	denotes	ATRX
T3347	17651-17658	NN	denotes	protein
T3349	17659-17662	CC	denotes	and
T3350	17663-17666	NN	denotes	DNA
T3351	17667-17678	NN	denotes	methylation
T3352	17679-17687	VBN	denotes	observed
T3353	17688-17690	IN	denotes	in
T3354	17691-17696	JJ	denotes	human
T3355	17697-17705	NNS	denotes	patients
T3341	17706-17708	VBZ	denotes	is
T3356	17709-17713	RB	denotes	also
T3357	17714-17721	JJ	denotes	present
T3358	17722-17724	IN	denotes	in
T3359	17725-17730	NN	denotes	mouse
T3360	17731-17736	NNS	denotes	cells
T3361	17736-17737	.	denotes	.
T3956	17739-17744	JJ	denotes	Early
T3958	17745-17754	JJ	denotes	Embryonic
T3957	17755-17764	NN	denotes	Lethality
T3959	17765-17767	IN	denotes	in
T3960	17768-17776	JJ	denotes	Atrxnull
T3962	17777-17781	JJ	denotes	Male
T3961	17782-17786	NNS	denotes	Mice
T3963	17786-17925	sentence	denotes	To investigate the role of the Atrx protein during mouse development, we initially established lines of mice bearing the Atrx flox allele.
T3964	17787-17789	TO	denotes	To
T3965	17790-17801	VB	denotes	investigate
T3967	17802-17805	DT	denotes	the
T3968	17806-17810	NN	denotes	role
T3969	17811-17813	IN	denotes	of
T3970	17814-17817	DT	denotes	the
T3972	17818-17822	NN	denotes	Atrx
T3971	17823-17830	NN	denotes	protein
T3973	17831-17837	IN	denotes	during
T3974	17838-17843	NN	denotes	mouse
T3975	17844-17855	NN	denotes	development
T3976	17855-17857	,	denotes	,
T3977	17857-17859	PRP	denotes	we
T3978	17860-17869	RB	denotes	initially
T3966	17870-17881	VBD	denotes	established
T3979	17882-17887	NNS	denotes	lines
T3980	17888-17890	IN	denotes	of
T3981	17891-17895	NNS	denotes	mice
T3982	17896-17903	VBG	denotes	bearing
T3983	17904-17907	DT	denotes	the
T3985	17908-17917	NN	denotes	Atrx flox
T3984	17918-17924	NN	denotes	allele
T3986	17924-17925	.	denotes	.
T3987	17925-18124	sentence	denotes	Two independently targeted Atrx flox ES cell clones with normal male karyotype were injected into C57BL/6 blastocysts to produce chimaeric mice, which were then used to obtain germline transmission.
T3988	17926-17929	CD	denotes	Two
T3990	17930-17943	RB	denotes	independently
T3991	17944-17952	VBN	denotes	targeted
T3992	17953-17962	NN	denotes	Atrx flox
T3993	17963-17965	NN	denotes	ES
T3994	17966-17970	NN	denotes	cell
T3989	17971-17977	NNS	denotes	clones
T3996	17978-17982	IN	denotes	with
T3997	17983-17989	JJ	denotes	normal
T3999	17990-17994	JJ	denotes	male
T3998	17995-18004	NN	denotes	karyotype
T4000	18005-18009	VBD	denotes	were
T3995	18010-18018	VBN	denotes	injected
T4001	18019-18023	IN	denotes	into
T4002	18024-18029	NN	denotes	C57BL
T4004	18029-18030	HYPH	denotes	/
T4005	18030-18031	CD	denotes	6
T4003	18032-18043	NNS	denotes	blastocysts
T4006	18044-18046	TO	denotes	to
T4007	18047-18054	VB	denotes	produce
T4008	18055-18064	JJ	denotes	chimaeric
T4009	18065-18069	NNS	denotes	mice
T4010	18069-18071	,	denotes	,
T4011	18071-18076	WDT	denotes	which
T4013	18077-18081	VBD	denotes	were
T4014	18082-18086	RB	denotes	then
T4012	18087-18091	VBN	denotes	used
T4015	18092-18094	TO	denotes	to
T4016	18095-18101	VB	denotes	obtain
T4017	18102-18110	NN	denotes	germline
T4018	18111-18123	NN	denotes	transmission
T4019	18123-18124	.	denotes	.
T4020	18124-18309	sentence	denotes	Intercrosses between males hemizygous (Atrx flox/Y) and females heterozygous (Atrx WT/flox) for the floxed allele were also carried out to generate homozygous females (Atrx flox/flox).
T4021	18125-18137	NNS	denotes	Intercrosses
T4023	18138-18145	IN	denotes	between
T4024	18146-18151	NNS	denotes	males
T4025	18152-18162	JJ	denotes	hemizygous
T4026	18163-18164	-LRB-	denotes	(
T4028	18164-18173	NN	denotes	Atrx flox
T4029	18173-18174	HYPH	denotes	/
T4027	18174-18175	NN	denotes	Y
T4030	18175-18176	-RRB-	denotes	)
T4031	18177-18180	CC	denotes	and
T4032	18181-18188	NNS	denotes	females
T4033	18189-18201	JJ	denotes	heterozygous
T4034	18202-18203	-LRB-	denotes	(
T4036	18203-18210	NN	denotes	Atrx WT
T4037	18210-18211	HYPH	denotes	/
T4035	18211-18215	NN	denotes	flox
T4038	18215-18216	-RRB-	denotes	)
T4039	18217-18220	IN	denotes	for
T4040	18221-18224	DT	denotes	the
T4042	18225-18231	VBN	denotes	floxed
T4041	18232-18238	NN	denotes	allele
T4043	18239-18243	VBD	denotes	were
T4044	18244-18248	RB	denotes	also
T4022	18249-18256	VBN	denotes	carried
T4045	18257-18260	RP	denotes	out
T4046	18261-18263	TO	denotes	to
T4047	18264-18272	VB	denotes	generate
T4048	18273-18283	JJ	denotes	homozygous
T4049	18284-18291	NNS	denotes	females
T4050	18292-18293	-LRB-	denotes	(
T4052	18293-18302	NN	denotes	Atrx flox
T4053	18302-18303	HYPH	denotes	/
T4051	18303-18307	NN	denotes	flox
T4054	18307-18308	-RRB-	denotes	)
T4055	18308-18309	.	denotes	.
T4056	18309-18516	sentence	denotes	Males hemizygous and females heterozygous or homozygous for the Atrx flox allele were viable, appeared healthy, and bred normally, suggesting that, as expected, the Atrx flox allele was functionally normal.
T4057	18310-18315	NNS	denotes	Males
T4059	18316-18326	JJ	denotes	hemizygous
T4060	18327-18330	CC	denotes	and
T4061	18331-18338	NNS	denotes	females
T4062	18339-18351	JJ	denotes	heterozygous
T4063	18352-18354	CC	denotes	or
T4064	18355-18365	JJ	denotes	homozygous
T4065	18366-18369	IN	denotes	for
T4066	18370-18373	DT	denotes	the
T4068	18374-18383	NN	denotes	Atrx flox
T4067	18384-18390	NN	denotes	allele
T4058	18391-18395	VBD	denotes	were
T4069	18396-18402	JJ	denotes	viable
T4070	18402-18404	,	denotes	,
T4071	18404-18412	VBD	denotes	appeared
T4072	18413-18420	JJ	denotes	healthy
T4073	18420-18422	,	denotes	,
T4074	18422-18425	CC	denotes	and
T4075	18426-18430	VBD	denotes	bred
T4076	18431-18439	RB	denotes	normally
T4077	18439-18441	,	denotes	,
T4078	18441-18451	VBG	denotes	suggesting
T4079	18452-18456	IN	denotes	that
T4081	18456-18458	,	denotes	,
T4082	18458-18460	IN	denotes	as
T4083	18461-18469	VBN	denotes	expected
T4084	18469-18471	,	denotes	,
T4085	18471-18474	DT	denotes	the
T4087	18475-18484	NN	denotes	Atrx flox
T4086	18485-18491	NN	denotes	allele
T4080	18492-18495	VBD	denotes	was
T4088	18496-18508	RB	denotes	functionally
T4089	18509-18515	JJ	denotes	normal
T4090	18515-18516	.	denotes	.
T4091	18516-18767	sentence	denotes	To generate Atrxnull mice by Cre-mediated recombination, the Atrx flox mice were crossed with mice harboring a transgene in which the Cre recombinase is expressed under the control of the regulatory elements of the mouse GATA-1 gene (GATA1-cre) [14].
T4092	18517-18519	TO	denotes	To
T4093	18520-18528	VB	denotes	generate
T4095	18529-18537	JJ	denotes	Atrxnull
T4096	18538-18542	NNS	denotes	mice
T4097	18543-18545	IN	denotes	by
T4098	18546-18549	NN	denotes	Cre
T4100	18549-18550	HYPH	denotes	-
T4099	18550-18558	VBN	denotes	mediated
T4101	18559-18572	NN	denotes	recombination
T4102	18572-18574	,	denotes	,
T4103	18574-18577	DT	denotes	the
T4105	18578-18587	NN	denotes	Atrx flox
T4104	18588-18592	NNS	denotes	mice
T4106	18593-18597	VBD	denotes	were
T4094	18598-18605	VBN	denotes	crossed
T4107	18606-18610	IN	denotes	with
T4108	18611-18615	NNS	denotes	mice
T4109	18616-18625	VBG	denotes	harboring
T4110	18626-18627	DT	denotes	a
T4111	18628-18637	NN	denotes	transgene
T4112	18638-18640	IN	denotes	in
T4114	18641-18646	WDT	denotes	which
T4115	18647-18650	DT	denotes	the
T4117	18651-18654	NN	denotes	Cre
T4116	18655-18666	NN	denotes	recombinase
T4118	18667-18669	VBZ	denotes	is
T4113	18670-18679	VBN	denotes	expressed
T4119	18680-18685	IN	denotes	under
T4120	18686-18689	DT	denotes	the
T4121	18690-18697	NN	denotes	control
T4122	18698-18700	IN	denotes	of
T4123	18701-18704	DT	denotes	the
T4125	18705-18715	JJ	denotes	regulatory
T4124	18716-18724	NNS	denotes	elements
T4126	18725-18727	IN	denotes	of
T4127	18728-18731	DT	denotes	the
T4129	18732-18737	NN	denotes	mouse
T4130	18738-18742	NN	denotes	GATA
T4131	18742-18743	HYPH	denotes	-
T4132	18743-18744	CD	denotes	1
T4128	18745-18749	NN	denotes	gene
T4133	18750-18751	-LRB-	denotes	(
T4134	18751-18756	NN	denotes	GATA1
T4136	18756-18757	HYPH	denotes	-
T4135	18757-18760	NN	denotes	cre
T4137	18760-18761	-RRB-	denotes	)
T4138	18762-18763	-LRB-	denotes	[
T4139	18763-18765	CD	denotes	14
T4140	18765-18766	-RRB-	denotes	]
T4141	18766-18767	.	denotes	.
T4142	18767-18871	sentence	denotes	Widespread expression of the GATA1-cre transgene has been demonstrated during early embryogenesis [14].
T4143	18768-18778	JJ	denotes	Widespread
T4144	18779-18789	NN	denotes	expression
T4146	18790-18792	IN	denotes	of
T4147	18793-18796	DT	denotes	the
T4149	18797-18802	NN	denotes	GATA1
T4151	18802-18803	HYPH	denotes	-
T4150	18803-18806	NN	denotes	cre
T4148	18807-18816	NN	denotes	transgene
T4152	18817-18820	VBZ	denotes	has
T4153	18821-18825	VBN	denotes	been
T4145	18826-18838	VBN	denotes	demonstrated
T4154	18839-18845	IN	denotes	during
T4155	18846-18851	JJ	denotes	early
T4156	18852-18865	NN	denotes	embryogenesis
T4157	18866-18867	-LRB-	denotes	[
T4158	18867-18869	CD	denotes	14
T4159	18869-18870	-RRB-	denotes	]
T4160	18870-18871	.	denotes	.
T4161	18871-19135	sentence	denotes	We more accurately defined the onset of GATA1-cre expression using a ROSA26 reporter strain, in which a β-galactosidase/neor fusion reporter gene is expressed only after Cre-mediated excision of loxP-flanked transcription and translation termination signals [14].
T4162	18872-18874	PRP	denotes	We
T4164	18875-18879	RBR	denotes	more
T4165	18880-18890	RB	denotes	accurately
T4163	18891-18898	VBN	denotes	defined
T4166	18899-18902	DT	denotes	the
T4167	18903-18908	NN	denotes	onset
T4168	18909-18911	IN	denotes	of
T4169	18912-18917	NN	denotes	GATA1
T4171	18917-18918	HYPH	denotes	-
T4170	18918-18921	NN	denotes	cre
T4172	18922-18932	NN	denotes	expression
T4173	18933-18938	VBG	denotes	using
T4174	18939-18940	DT	denotes	a
T4176	18941-18947	NN	denotes	ROSA26
T4177	18948-18956	NN	denotes	reporter
T4175	18957-18963	NN	denotes	strain
T4178	18963-18965	,	denotes	,
T4179	18965-18967	IN	denotes	in
T4181	18968-18973	WDT	denotes	which
T4182	18974-18975	DT	denotes	a
T4184	18976-18977	NN	denotes	β
T4186	18977-18978	HYPH	denotes	-
T4185	18978-18991	NN	denotes	galactosidase
T4188	18991-18992	HYPH	denotes	/
T4189	18992-18996	NN	denotes	neor
T4187	18997-19003	NN	denotes	fusion
T4190	19004-19012	NN	denotes	reporter
T4183	19013-19017	NN	denotes	gene
T4191	19018-19020	VBZ	denotes	is
T4180	19021-19030	VBN	denotes	expressed
T4192	19031-19035	RB	denotes	only
T4193	19036-19041	IN	denotes	after
T4194	19042-19045	NN	denotes	Cre
T4196	19045-19046	HYPH	denotes	-
T4195	19046-19054	VBN	denotes	mediated
T4197	19055-19063	NN	denotes	excision
T4198	19064-19066	IN	denotes	of
T4199	19067-19071	NN	denotes	loxP
T4201	19071-19072	HYPH	denotes	-
T4200	19072-19079	VBN	denotes	flanked
T4202	19080-19093	NN	denotes	transcription
T4203	19094-19097	CC	denotes	and
T4204	19098-19109	NN	denotes	translation
T4205	19110-19121	NN	denotes	termination
T4206	19122-19129	NNS	denotes	signals
T4207	19130-19131	-LRB-	denotes	[
T4208	19131-19133	CD	denotes	14
T4209	19133-19134	-RRB-	denotes	]
T4210	19134-19135	.	denotes	.
T4211	19135-19276	sentence	denotes	We found that the GATA1-cre transgene was already active at the 16-cell morula stage of development (0.5 days postcoitus [dpc]) (Figure 4A).
T4212	19136-19138	PRP	denotes	We
T4213	19139-19144	VBD	denotes	found
T4214	19145-19149	IN	denotes	that
T4216	19150-19153	DT	denotes	the
T4218	19154-19159	NN	denotes	GATA1
T4220	19159-19160	HYPH	denotes	-
T4219	19160-19163	NN	denotes	cre
T4217	19164-19173	NN	denotes	transgene
T4215	19174-19177	VBD	denotes	was
T4221	19178-19185	RB	denotes	already
T4222	19186-19192	JJ	denotes	active
T4223	19193-19195	IN	denotes	at
T4224	19196-19199	DT	denotes	the
T4226	19200-19202	CD	denotes	16
T4228	19202-19203	HYPH	denotes	-
T4227	19203-19207	NN	denotes	cell
T4229	19208-19214	NN	denotes	morula
T4225	19215-19220	NN	denotes	stage
T4230	19221-19223	IN	denotes	of
T4231	19224-19235	NN	denotes	development
T4232	19236-19237	-LRB-	denotes	(
T4233	19237-19240	CD	denotes	0.5
T4234	19241-19245	NNS	denotes	days
T4235	19246-19256	RB	denotes	postcoitus
T4236	19257-19258	-LRB-	denotes	[
T4237	19258-19261	NN	denotes	dpc
T4238	19261-19262	-RRB-	denotes	]
T4239	19262-19263	-RRB-	denotes	)
T4240	19264-19265	-LRB-	denotes	(
T4242	19265-19271	NN	denotes	Figure
T4241	19272-19274	NN	denotes	4A
T4243	19274-19275	-RRB-	denotes	)
T4244	19275-19276	.	denotes	.
T4245	19276-20448	sentence	denotes	Figure 4 Timing of Onset of GATA1-Cre Expression and PCR Genotyping of Atrx Alleles (A) GATA1-cre +/+ transgenic males were crossed to females of the ROSA26 reporter strain (ROSA26 +/−), and embryos were recovered at 0.5 dpc (~16-cell morula stage) and stained with X-gal. Cre-mediated activation of the ROSA26 β-galactosidase reporter allele was detected in all cells in embryos in which both alleles are coinherited. (B) Top gel: PCR genotyping of Atrx alleles in embryos using primers PPS1.15 (exon 17) and Mxnp30 (exon 20) as described in Protocol S1. The sizes of PCR products from the different alleles are indicated. Both the Atrx Δ18 (resulting from recombination event B in Figure 2A) and the Atrx Δ18Δneo allele (resulting from recombination event C in Figure 2A) are null for full-length Atrx protein. The bottom gel shows products from a PCR reaction (primers DG52/DG53) used to sex embryos as described in Protocol S1. A 450-bp PCR product is amplified from a mouse Y chromosome-specific satellite repeat. To generate Atrxnull mice, heterozygous floxed females (Atrx WT/flox) were mated with homozygous GATA1-cre transgenic males (Atrx WT/Y;GATA1-cre +/+ ).
T4246	20297-20299	TO	denotes	To
T4247	20300-20308	VB	denotes	generate
T4249	20309-20317	JJ	denotes	Atrxnull
T4250	20318-20322	NNS	denotes	mice
T4251	20322-20324	,	denotes	,
T4252	20324-20336	JJ	denotes	heterozygous
T4254	20337-20343	VBN	denotes	floxed
T4253	20344-20351	NNS	denotes	females
T4255	20352-20353	-LRB-	denotes	(
T4257	20353-20360	NN	denotes	Atrx WT
T4258	20360-20361	HYPH	denotes	/
T4256	20361-20365	NN	denotes	flox
T4259	20365-20366	-RRB-	denotes	)
T4260	20367-20371	VBD	denotes	were
T4248	20372-20377	VBN	denotes	mated
T4261	20378-20382	IN	denotes	with
T4262	20383-20393	JJ	denotes	homozygous
T4264	20394-20399	NN	denotes	GATA1
T4266	20399-20400	HYPH	denotes	-
T4265	20400-20403	NN	denotes	cre
T4267	20404-20414	JJ	denotes	transgenic
T4263	20415-20420	NNS	denotes	males
T4268	20421-20422	-LRB-	denotes	(
T4270	20422-20429	NN	denotes	Atrx WT
T4272	20429-20430	HYPH	denotes	/
T4271	20430-20431	NN	denotes	Y
T4273	20431-20432	:	denotes	;
T4274	20432-20437	NN	denotes	GATA1
T4275	20437-20438	HYPH	denotes	-
T4269	20438-20441	NN	denotes	cre
T4276	20442-20443	SYM	denotes	+
T4277	20443-20444	HYPH	denotes	/
T4278	20444-20445	SYM	denotes	+
T4279	20446-20447	-RRB-	denotes	)
T4280	20447-20448	.	denotes	.
T4281	20448-20588	sentence	denotes	No Atrxnull males (Atrx null/Y;GATA1-cre +/− ) were recovered at birth, indicating that the absence of Atrx results in embryonic lethality.
T4282	20449-20451	DT	denotes	No
T4284	20452-20460	JJ	denotes	Atrxnull
T4283	20461-20466	NNS	denotes	males
T4286	20467-20468	-LRB-	denotes	(
T4288	20468-20477	NN	denotes	Atrx null
T4290	20477-20478	HYPH	denotes	/
T4289	20478-20479	NN	denotes	Y
T4291	20479-20480	:	denotes	;
T4292	20480-20485	NN	denotes	GATA1
T4293	20485-20486	HYPH	denotes	-
T4287	20486-20489	NN	denotes	cre
T4294	20490-20491	SYM	denotes	+
T4295	20491-20492	HYPH	denotes	/
T4296	20492-20493	SYM	denotes	−
T4297	20494-20495	-RRB-	denotes	)
T4298	20496-20500	VBD	denotes	were
T4285	20501-20510	VBN	denotes	recovered
T4299	20511-20513	IN	denotes	at
T4300	20514-20519	NN	denotes	birth
T4301	20519-20521	,	denotes	,
T4302	20521-20531	VBG	denotes	indicating
T4303	20532-20536	IN	denotes	that
T4305	20537-20540	DT	denotes	the
T4306	20541-20548	NN	denotes	absence
T4307	20549-20551	IN	denotes	of
T4308	20552-20556	NN	denotes	Atrx
T4304	20557-20564	VBZ	denotes	results
T4309	20565-20567	IN	denotes	in
T4310	20568-20577	JJ	denotes	embryonic
T4311	20578-20587	NN	denotes	lethality
T4312	20587-20588	.	denotes	.
T4313	20588-20691	sentence	denotes	This finding was unexpected, since human ATR-X patients clearly survive to adulthood (see Discussion).
T4314	20589-20593	DT	denotes	This
T4315	20594-20601	NN	denotes	finding
T4316	20602-20605	VBD	denotes	was
T4317	20606-20616	JJ	denotes	unexpected
T4318	20616-20618	,	denotes	,
T4319	20618-20623	IN	denotes	since
T4321	20624-20629	JJ	denotes	human
T4323	20630-20633	NN	denotes	ATR
T4325	20633-20634	HYPH	denotes	-
T4324	20634-20635	NN	denotes	X
T4322	20636-20644	NNS	denotes	patients
T4326	20645-20652	RB	denotes	clearly
T4320	20653-20660	VBP	denotes	survive
T4327	20661-20663	IN	denotes	to
T4328	20664-20673	NN	denotes	adulthood
T4329	20674-20675	-LRB-	denotes	(
T4330	20675-20678	VB	denotes	see
T4331	20679-20689	NN	denotes	Discussion
T4332	20689-20690	-RRB-	denotes	)
T4333	20690-20691	.	denotes	.
T4334	20691-20845	sentence	denotes	Embryos were dissected at 7.5, 8.5, and 9.5 dpc and genotyped by PCR analysis of DNA extracted from yolk sac or total embryo (Figure 4B and Protocol S1).
T4335	20692-20699	NNS	denotes	Embryos
T4337	20700-20704	VBD	denotes	were
T4336	20705-20714	VBN	denotes	dissected
T4338	20715-20717	IN	denotes	at
T4339	20718-20721	CD	denotes	7.5
T4341	20721-20723	,	denotes	,
T4342	20723-20726	CD	denotes	8.5
T4343	20726-20728	,	denotes	,
T4344	20728-20731	CC	denotes	and
T4345	20732-20735	CD	denotes	9.5
T4340	20736-20739	NN	denotes	dpc
T4346	20740-20743	CC	denotes	and
T4347	20744-20753	VBN	denotes	genotyped
T4348	20754-20756	IN	denotes	by
T4349	20757-20760	NN	denotes	PCR
T4350	20761-20769	NN	denotes	analysis
T4351	20770-20772	IN	denotes	of
T4352	20773-20776	NN	denotes	DNA
T4353	20777-20786	VBN	denotes	extracted
T4354	20787-20791	IN	denotes	from
T4355	20792-20796	NN	denotes	yolk
T4356	20797-20800	NN	denotes	sac
T4357	20801-20803	CC	denotes	or
T4358	20804-20809	JJ	denotes	total
T4359	20810-20816	NN	denotes	embryo
T4360	20817-20818	-LRB-	denotes	(
T4362	20818-20824	NN	denotes	Figure
T4361	20825-20827	NN	denotes	4B
T4363	20828-20831	CC	denotes	and
T4364	20832-20840	NN	denotes	Protocol
T4365	20841-20843	NN	denotes	S1
T4366	20843-20844	-RRB-	denotes	)
T4367	20844-20845	.	denotes	.
T4368	20845-20948	sentence	denotes	Atrxnull males were present at expected mendelian ratios (~25%) at both 7.5 dpc and 8.5 dpc (Table 1).
T4369	20846-20854	JJ	denotes	Atrxnull
T4370	20855-20860	NNS	denotes	males
T4371	20861-20865	VBD	denotes	were
T4372	20866-20873	JJ	denotes	present
T4373	20874-20876	IN	denotes	at
T4374	20877-20885	VBN	denotes	expected
T4376	20886-20895	JJ	denotes	mendelian
T4375	20896-20902	NNS	denotes	ratios
T4377	20903-20904	-LRB-	denotes	(
T4379	20904-20905	SYM	denotes	~
T4380	20905-20907	CD	denotes	25
T4378	20907-20908	NN	denotes	%
T4381	20908-20909	-RRB-	denotes	)
T4382	20910-20912	IN	denotes	at
T4383	20913-20917	CC	denotes	both
T4385	20918-20921	CD	denotes	7.5
T4384	20922-20925	NN	denotes	dpc
T4386	20926-20929	CC	denotes	and
T4387	20930-20933	CD	denotes	8.5
T4388	20934-20937	NN	denotes	dpc
T4389	20938-20939	-LRB-	denotes	(
T4390	20939-20944	NN	denotes	Table
T4391	20945-20946	CD	denotes	1
T4392	20946-20947	-RRB-	denotes	)
T4393	20947-20948	.	denotes	.
T4394	20948-21084	sentence	denotes	However, by 9.5 dpc, depletion was observed both in the number of Atrxnull males (7%) and in the total number of males recovered (31%).
T4395	20949-20956	RB	denotes	However
T4397	20956-20958	,	denotes	,
T4398	20958-20960	IN	denotes	by
T4399	20961-20964	CD	denotes	9.5
T4400	20965-20968	NN	denotes	dpc
T4401	20968-20970	,	denotes	,
T4402	20970-20979	NN	denotes	depletion
T4403	20980-20983	VBD	denotes	was
T4396	20984-20992	VBN	denotes	observed
T4404	20993-20997	CC	denotes	both
T4405	20998-21000	IN	denotes	in
T4406	21001-21004	DT	denotes	the
T4407	21005-21011	NN	denotes	number
T4408	21012-21014	IN	denotes	of
T4409	21015-21023	JJ	denotes	Atrxnull
T4410	21024-21029	NNS	denotes	males
T4411	21030-21031	-LRB-	denotes	(
T4413	21031-21032	CD	denotes	7
T4412	21032-21033	NN	denotes	%
T4414	21033-21034	-RRB-	denotes	)
T4415	21035-21038	CC	denotes	and
T4416	21039-21041	IN	denotes	in
T4417	21042-21045	DT	denotes	the
T4419	21046-21051	JJ	denotes	total
T4418	21052-21058	NN	denotes	number
T4420	21059-21061	IN	denotes	of
T4421	21062-21067	NNS	denotes	males
T4422	21068-21077	VBN	denotes	recovered
T4423	21078-21079	-LRB-	denotes	(
T4425	21079-21081	CD	denotes	31
T4424	21081-21082	NN	denotes	%
T4426	21082-21083	-RRB-	denotes	)
T4427	21083-21084	.	denotes	.
T4428	21084-21132	sentence	denotes	No Atrxnull males were recovered after 9.5 dpc.
T4429	21085-21087	DT	denotes	No
T4431	21088-21096	JJ	denotes	Atrxnull
T4430	21097-21102	NNS	denotes	males
T4433	21103-21107	VBD	denotes	were
T4432	21108-21117	VBN	denotes	recovered
T4434	21118-21123	IN	denotes	after
T4435	21124-21127	CD	denotes	9.5
T4436	21128-21131	NN	denotes	dpc
T4437	21131-21132	.	denotes	.
T4438	21132-21215	sentence	denotes	Thus the absence of Atrx gives rise to embryonic lethality in mice before 9.5 dpc.
T4439	21133-21137	RB	denotes	Thus
T4441	21138-21141	DT	denotes	the
T4442	21142-21149	NN	denotes	absence
T4443	21150-21152	IN	denotes	of
T4444	21153-21157	NN	denotes	Atrx
T4440	21158-21163	VBZ	denotes	gives
T4445	21164-21168	NN	denotes	rise
T4446	21169-21171	IN	denotes	to
T4447	21172-21181	JJ	denotes	embryonic
T4448	21182-21191	NN	denotes	lethality
T4449	21192-21194	IN	denotes	in
T4450	21195-21199	NNS	denotes	mice
T4451	21200-21206	IN	denotes	before
T4452	21207-21210	CD	denotes	9.5
T4453	21211-21214	NN	denotes	dpc
T4454	21214-21215	.	denotes	.
T4455	21215-21535	sentence	denotes	Table 1 Distribution of Atrx Genotypes in Timed Matings To investigate the morphology of Atrxnull embryos prior to death, embryos from the above crosses were initially dissected in their deciduas at 7.5 dpc, and paraffin sections were stained with haematoxylin (Figure 5A) or with an anti-ATRX antibody (Figure 5B–5E).
T4456	21216-21275	TO	denotes	Table 1 Distribution of Atrx Genotypes in Timed Matings To
T4457	21276-21287	VB	denotes	investigate
T4459	21288-21291	DT	denotes	the
T4460	21292-21302	NN	denotes	morphology
T4461	21303-21305	IN	denotes	of
T4462	21306-21314	JJ	denotes	Atrxnull
T4463	21315-21322	NNS	denotes	embryos
T4464	21323-21328	RB	denotes	prior
T4465	21329-21331	IN	denotes	to
T4466	21332-21337	NN	denotes	death
T4467	21337-21339	,	denotes	,
T4468	21339-21346	NNS	denotes	embryos
T4469	21347-21351	IN	denotes	from
T4470	21352-21355	DT	denotes	the
T4472	21356-21361	JJ	denotes	above
T4471	21362-21369	NNS	denotes	crosses
T4473	21370-21374	VBD	denotes	were
T4474	21375-21384	RB	denotes	initially
T4458	21385-21394	VBN	denotes	dissected
T4475	21395-21397	IN	denotes	in
T4476	21398-21403	PRP$	denotes	their
T4477	21404-21412	NNS	denotes	deciduas
T4478	21413-21415	IN	denotes	at
T4479	21416-21419	CD	denotes	7.5
T4480	21420-21423	NN	denotes	dpc
T4481	21423-21425	,	denotes	,
T4482	21425-21428	CC	denotes	and
T4483	21429-21437	NN	denotes	paraffin
T4484	21438-21446	NNS	denotes	sections
T4486	21447-21451	VBD	denotes	were
T4485	21452-21459	VBN	denotes	stained
T4487	21460-21464	IN	denotes	with
T4488	21465-21477	NN	denotes	haematoxylin
T4489	21478-21479	-LRB-	denotes	(
T4491	21479-21485	NN	denotes	Figure
T4490	21486-21488	NN	denotes	5A
T4492	21488-21489	-RRB-	denotes	)
T4493	21490-21492	CC	denotes	or
T4494	21493-21497	IN	denotes	with
T4495	21498-21500	DT	denotes	an
T4497	21501-21510	JJ	denotes	anti-ATRX
T4496	21511-21519	NN	denotes	antibody
T4498	21520-21521	-LRB-	denotes	(
T4500	21521-21527	NN	denotes	Figure
T4499	21528-21530	NN	denotes	5B
T4501	21530-21531	SYM	denotes	–
T4502	21531-21533	NN	denotes	5E
T4503	21533-21534	-RRB-	denotes	)
T4504	21534-21535	.	denotes	.
T4505	21535-21646	sentence	denotes	Immunohistochemical staining revealed that Atrx was widely expressed in wild-type 7.5 dpc embryos (Figure 5B).
T4506	21536-21555	JJ	denotes	Immunohistochemical
T4507	21556-21564	NN	denotes	staining
T4508	21565-21573	VBD	denotes	revealed
T4509	21574-21578	IN	denotes	that
T4511	21579-21583	NN	denotes	Atrx
T4512	21584-21587	VBD	denotes	was
T4513	21588-21594	RB	denotes	widely
T4510	21595-21604	VBN	denotes	expressed
T4514	21605-21607	IN	denotes	in
T4515	21608-21612	JJ	denotes	wild
T4517	21612-21613	HYPH	denotes	-
T4516	21613-21617	NN	denotes	type
T4519	21618-21621	CD	denotes	7.5
T4520	21622-21625	NN	denotes	dpc
T4518	21626-21633	NNS	denotes	embryos
T4521	21634-21635	-LRB-	denotes	(
T4523	21635-21641	NN	denotes	Figure
T4522	21642-21644	NN	denotes	5B
T4524	21644-21645	-RRB-	denotes	)
T4525	21645-21646	.	denotes	.
T4526	21646-21734	sentence	denotes	Expression was highest in the embryonic region (Figure 5C) and the chorion (Figure 5D).
T4527	21647-21657	NN	denotes	Expression
T4528	21658-21661	VBD	denotes	was
T4529	21662-21669	JJS	denotes	highest
T4530	21670-21672	IN	denotes	in
T4531	21673-21676	DT	denotes	the
T4533	21677-21686	JJ	denotes	embryonic
T4532	21687-21693	NN	denotes	region
T4534	21694-21695	-LRB-	denotes	(
T4536	21695-21701	NN	denotes	Figure
T4535	21702-21704	NN	denotes	5C
T4537	21704-21705	-RRB-	denotes	)
T4538	21706-21709	CC	denotes	and
T4539	21710-21713	DT	denotes	the
T4540	21714-21721	NN	denotes	chorion
T4541	21722-21723	-LRB-	denotes	(
T4543	21723-21729	NN	denotes	Figure
T4542	21730-21732	NN	denotes	5D
T4544	21732-21733	-RRB-	denotes	)
T4545	21733-21734	.	denotes	.
T4546	21734-21861	sentence	denotes	Detectable but lower levels of expression were observed in the ectoplacental cone (Figure 5D) and surrounding decidual tissue.
T4547	21735-21745	JJ	denotes	Detectable
T4549	21746-21749	CC	denotes	but
T4550	21750-21755	JJR	denotes	lower
T4548	21756-21762	NNS	denotes	levels
T4552	21763-21765	IN	denotes	of
T4553	21766-21776	NN	denotes	expression
T4554	21777-21781	VBD	denotes	were
T4551	21782-21790	VBN	denotes	observed
T4555	21791-21793	IN	denotes	in
T4556	21794-21797	DT	denotes	the
T4558	21798-21811	JJ	denotes	ectoplacental
T4557	21812-21816	NN	denotes	cone
T4559	21817-21818	-LRB-	denotes	(
T4561	21818-21824	NN	denotes	Figure
T4560	21825-21827	NN	denotes	5D
T4562	21827-21828	-RRB-	denotes	)
T4563	21829-21832	CC	denotes	and
T4564	21833-21844	VBG	denotes	surrounding
T4566	21845-21853	JJ	denotes	decidual
T4565	21854-21860	NN	denotes	tissue
T4567	21860-21861	.	denotes	.
T4568	21861-21997	sentence	denotes	We also observed very high levels of Atrx expression in trophoblast giant cells (TGCs) surrounding the Reichert's membrane (Figure 5E).
T4569	21862-21864	PRP	denotes	We
T4571	21865-21869	RB	denotes	also
T4570	21870-21878	VBD	denotes	observed
T4572	21879-21883	RB	denotes	very
T4573	21884-21888	JJ	denotes	high
T4574	21889-21895	NNS	denotes	levels
T4575	21896-21898	IN	denotes	of
T4576	21899-21903	NN	denotes	Atrx
T4577	21904-21914	NN	denotes	expression
T4578	21915-21917	IN	denotes	in
T4579	21918-21929	NN	denotes	trophoblast
T4581	21930-21935	JJ	denotes	giant
T4580	21936-21941	NNS	denotes	cells
T4582	21942-21943	-LRB-	denotes	(
T4583	21943-21947	NNS	denotes	TGCs
T4584	21947-21948	-RRB-	denotes	)
T4585	21949-21960	VBG	denotes	surrounding
T4586	21961-21964	DT	denotes	the
T4588	21965-21973	NN	denotes	Reichert
T4589	21973-21975	POS	denotes	's
T4587	21976-21984	NN	denotes	membrane
T4590	21985-21986	-LRB-	denotes	(
T4592	21986-21992	NN	denotes	Figure
T4591	21993-21995	NN	denotes	5E
T4593	21995-21996	-RRB-	denotes	)
T4594	21996-21997	.	denotes	.
T4595	21997-22152	sentence	denotes	Within the large nuclei of these TGCs, the typical nuclear association of Atrx with blocks of pericentromeric heterochromatin [15] was clearly observable.
T4596	21998-22004	IN	denotes	Within
T4598	22005-22008	DT	denotes	the
T4600	22009-22014	JJ	denotes	large
T4599	22015-22021	NNS	denotes	nuclei
T4601	22022-22024	IN	denotes	of
T4602	22025-22030	DT	denotes	these
T4603	22031-22035	NNS	denotes	TGCs
T4604	22035-22037	,	denotes	,
T4605	22037-22040	DT	denotes	the
T4607	22041-22048	JJ	denotes	typical
T4608	22049-22056	JJ	denotes	nuclear
T4606	22057-22068	NN	denotes	association
T4609	22069-22071	IN	denotes	of
T4610	22072-22076	NN	denotes	Atrx
T4611	22077-22081	IN	denotes	with
T4612	22082-22088	NNS	denotes	blocks
T4613	22089-22091	IN	denotes	of
T4614	22092-22107	JJ	denotes	pericentromeric
T4615	22108-22123	NN	denotes	heterochromatin
T4616	22124-22125	-LRB-	denotes	[
T4617	22125-22127	CD	denotes	15
T4618	22127-22128	-RRB-	denotes	]
T4597	22129-22132	VBD	denotes	was
T4619	22133-22140	RB	denotes	clearly
T4620	22141-22151	JJ	denotes	observable
T4621	22151-22152	.	denotes	.
T4622	22152-22397	sentence	denotes	Only background staining was seen in the corresponding Atrxnull embryonic tissues (Figure 5B–5D), while expression in the surrounding decidual tissue (of maternal origin) was normal and served as an antibody staining control (unpublished data).
T4623	22153-22157	RB	denotes	Only
T4625	22158-22168	NN	denotes	background
T4624	22169-22177	NN	denotes	staining
T4627	22178-22181	VBD	denotes	was
T4626	22182-22186	VBN	denotes	seen
T4628	22187-22189	IN	denotes	in
T4629	22190-22193	DT	denotes	the
T4631	22194-22207	VBG	denotes	corresponding
T4632	22208-22216	JJ	denotes	Atrxnull
T4633	22217-22226	JJ	denotes	embryonic
T4630	22227-22234	NNS	denotes	tissues
T4634	22235-22236	-LRB-	denotes	(
T4636	22236-22242	NN	denotes	Figure
T4635	22243-22245	NN	denotes	5B
T4637	22245-22246	SYM	denotes	–
T4638	22246-22248	NN	denotes	5D
T4639	22248-22249	-RRB-	denotes	)
T4640	22249-22251	,	denotes	,
T4641	22251-22256	IN	denotes	while
T4643	22257-22267	NN	denotes	expression
T4644	22268-22270	IN	denotes	in
T4645	22271-22274	DT	denotes	the
T4647	22275-22286	VBG	denotes	surrounding
T4648	22287-22295	JJ	denotes	decidual
T4646	22296-22302	NN	denotes	tissue
T4649	22303-22304	-LRB-	denotes	(
T4650	22304-22306	IN	denotes	of
T4651	22307-22315	JJ	denotes	maternal
T4652	22316-22322	NN	denotes	origin
T4653	22322-22323	-RRB-	denotes	)
T4642	22324-22327	VBD	denotes	was
T4654	22328-22334	JJ	denotes	normal
T4655	22335-22338	CC	denotes	and
T4656	22339-22345	VBD	denotes	served
T4657	22346-22348	IN	denotes	as
T4658	22349-22351	DT	denotes	an
T4660	22352-22360	NN	denotes	antibody
T4661	22361-22369	NN	denotes	staining
T4659	22370-22377	NN	denotes	control
T4662	22378-22379	-LRB-	denotes	(
T4664	22379-22390	JJ	denotes	unpublished
T4663	22391-22395	NNS	denotes	data
T4665	22395-22396	-RRB-	denotes	)
T4666	22396-22397	.	denotes	.
T4667	22397-22575	sentence	denotes	Morphologically, 7.5 dpc Atrxnull embryos were dramatically reduced in size and appeared developmentally retarded relative to stage-matched wild-type embryos (Figure 5A and 5B).
T4668	22398-22413	RB	denotes	Morphologically
T4670	22413-22415	,	denotes	,
T4671	22415-22418	CD	denotes	7.5
T4672	22419-22422	NNS	denotes	dpc
T4674	22423-22431	JJ	denotes	Atrxnull
T4673	22432-22439	NNS	denotes	embryos
T4675	22440-22444	VBD	denotes	were
T4676	22445-22457	RB	denotes	dramatically
T4669	22458-22465	VBN	denotes	reduced
T4677	22466-22468	IN	denotes	in
T4678	22469-22473	NN	denotes	size
T4679	22474-22477	CC	denotes	and
T4680	22478-22486	VBD	denotes	appeared
T4681	22487-22502	RB	denotes	developmentally
T4682	22503-22511	JJ	denotes	retarded
T4683	22512-22520	JJ	denotes	relative
T4684	22521-22523	IN	denotes	to
T4685	22524-22529	NN	denotes	stage
T4687	22529-22530	HYPH	denotes	-
T4686	22530-22537	VBN	denotes	matched
T4689	22538-22542	JJ	denotes	wild
T4691	22542-22543	HYPH	denotes	-
T4690	22543-22547	NN	denotes	type
T4688	22548-22555	NNS	denotes	embryos
T4692	22556-22557	-LRB-	denotes	(
T4694	22557-22563	NN	denotes	Figure
T4693	22564-22566	NN	denotes	5A
T4695	22567-22570	CC	denotes	and
T4696	22571-22573	NN	denotes	5B
T4697	22573-22574	-RRB-	denotes	)
T4698	22574-22575	.	denotes	.
T4699	22575-22725	sentence	denotes	However, despite their reduced size, the general morphology and organisation of embryonic structures in Atrxnull conceptuses appeared grossly normal.
T4700	22576-22583	RB	denotes	However
T4702	22583-22585	,	denotes	,
T4703	22585-22592	IN	denotes	despite
T4704	22593-22598	PRP$	denotes	their
T4706	22599-22606	VBN	denotes	reduced
T4705	22607-22611	NN	denotes	size
T4707	22611-22613	,	denotes	,
T4708	22613-22616	DT	denotes	the
T4710	22617-22624	JJ	denotes	general
T4709	22625-22635	NN	denotes	morphology
T4711	22636-22639	CC	denotes	and
T4712	22640-22652	NN	denotes	organisation
T4713	22653-22655	IN	denotes	of
T4714	22656-22665	JJ	denotes	embryonic
T4715	22666-22676	NNS	denotes	structures
T4716	22677-22679	IN	denotes	in
T4717	22680-22688	JJ	denotes	Atrxnull
T4718	22689-22700	NNS	denotes	conceptuses
T4701	22701-22709	VBD	denotes	appeared
T4719	22710-22717	RB	denotes	grossly
T4720	22718-22724	JJ	denotes	normal
T4721	22724-22725	.	denotes	.
T4722	22725-22905	sentence	denotes	The amnion and chorion were clearly present and the amniotic, exocoelomic, and ectoplacental cavities were distinguishable, as were all three embryonic germ layers (Figure 5A–5C).
T4723	22726-22729	DT	denotes	The
T4724	22730-22736	NN	denotes	amnion
T4726	22737-22740	CC	denotes	and
T4727	22741-22748	NN	denotes	chorion
T4725	22749-22753	VBD	denotes	were
T4728	22754-22761	RB	denotes	clearly
T4729	22762-22769	JJ	denotes	present
T4730	22770-22773	CC	denotes	and
T4731	22774-22777	DT	denotes	the
T4733	22778-22786	JJ	denotes	amniotic
T4734	22786-22788	,	denotes	,
T4735	22788-22799	JJ	denotes	exocoelomic
T4736	22799-22801	,	denotes	,
T4737	22801-22804	CC	denotes	and
T4738	22805-22818	JJ	denotes	ectoplacental
T4732	22819-22827	NNS	denotes	cavities
T4739	22828-22832	VBD	denotes	were
T4740	22833-22848	JJ	denotes	distinguishable
T4741	22848-22850	,	denotes	,
T4742	22850-22852	IN	denotes	as
T4743	22853-22857	VBD	denotes	were
T4744	22858-22861	DT	denotes	all
T4746	22862-22867	CD	denotes	three
T4747	22868-22877	JJ	denotes	embryonic
T4748	22878-22882	NN	denotes	germ
T4745	22883-22889	NNS	denotes	layers
T4749	22890-22891	-LRB-	denotes	(
T4751	22891-22897	NN	denotes	Figure
T4750	22898-22900	NN	denotes	5A
T4752	22900-22901	SYM	denotes	–
T4753	22901-22903	NN	denotes	5C
T4754	22903-22904	-RRB-	denotes	)
T4755	22904-22905	.	denotes	.
T4756	22905-22987	sentence	denotes	At 8.5 dpc, embryos were dissected free of deciduas, and observed in whole mount.
T4757	22906-22908	IN	denotes	At
T4759	22909-22912	CD	denotes	8.5
T4760	22913-22916	NN	denotes	dpc
T4761	22916-22918	,	denotes	,
T4762	22918-22925	NNS	denotes	embryos
T4763	22926-22930	VBD	denotes	were
T4758	22931-22940	VBN	denotes	dissected
T4764	22941-22945	JJ	denotes	free
T4765	22946-22948	IN	denotes	of
T4766	22949-22957	NNS	denotes	deciduas
T4767	22957-22959	,	denotes	,
T4768	22959-22962	CC	denotes	and
T4769	22963-22971	VBN	denotes	observed
T4770	22972-22974	IN	denotes	in
T4771	22975-22980	JJ	denotes	whole
T4772	22981-22986	NN	denotes	mount
T4773	22986-22987	.	denotes	.
T4774	22987-23094	sentence	denotes	Individual conceptuses were genotyped by PCR using DNA isolated from yolk sac as described in Protocol S1.
T4775	22988-22998	JJ	denotes	Individual
T4776	22999-23010	NNS	denotes	conceptuses
T4778	23011-23015	VBD	denotes	were
T4777	23016-23025	VBN	denotes	genotyped
T4779	23026-23028	IN	denotes	by
T4780	23029-23032	NN	denotes	PCR
T4781	23033-23038	VBG	denotes	using
T4782	23039-23042	NN	denotes	DNA
T4783	23043-23051	VBN	denotes	isolated
T4784	23052-23056	IN	denotes	from
T4785	23057-23061	NN	denotes	yolk
T4786	23062-23065	NN	denotes	sac
T4787	23066-23068	IN	denotes	as
T4788	23069-23078	VBN	denotes	described
T4789	23079-23081	IN	denotes	in
T4790	23082-23090	NN	denotes	Protocol
T4791	23091-23093	NN	denotes	S1
T4792	23093-23094	.	denotes	.
T4793	23094-23244	sentence	denotes	Consistent with observations at 7.5 dpc, the general morphology of the embryo proper of Atrxnull conceptuses also appeared grossly normal at 8.5 dpc.
T4794	23095-23105	JJ	denotes	Consistent
T4796	23106-23110	IN	denotes	with
T4797	23111-23123	NNS	denotes	observations
T4798	23124-23126	IN	denotes	at
T4799	23127-23130	CD	denotes	7.5
T4800	23131-23134	NN	denotes	dpc
T4801	23134-23136	,	denotes	,
T4802	23136-23139	DT	denotes	the
T4804	23140-23147	JJ	denotes	general
T4803	23148-23158	NN	denotes	morphology
T4805	23159-23161	IN	denotes	of
T4806	23162-23165	DT	denotes	the
T4807	23166-23172	NN	denotes	embryo
T4808	23173-23179	JJ	denotes	proper
T4809	23180-23182	IN	denotes	of
T4810	23183-23191	JJ	denotes	Atrxnull
T4811	23192-23203	NNS	denotes	conceptuses
T4812	23204-23208	RB	denotes	also
T4795	23209-23217	VBD	denotes	appeared
T4813	23218-23225	RB	denotes	grossly
T4814	23226-23232	JJ	denotes	normal
T4815	23233-23235	IN	denotes	at
T4816	23236-23239	CD	denotes	8.5
T4817	23240-23243	NN	denotes	dpc
T4818	23243-23244	.	denotes	.
T4819	23244-23509	sentence	denotes	The head fold had clearly formed, and expression of the early mesoderm marker brachyury (T) [16] was detected in the primitive streak and emerging notochord by whole-mount in situ hybridisation (WMISH) (Figure 5F), indicating that Atrxnull embryos had gastrulated.
T4820	23245-23248	DT	denotes	The
T4822	23249-23253	NN	denotes	head
T4821	23254-23258	NN	denotes	fold
T4824	23259-23262	VBD	denotes	had
T4825	23263-23270	RB	denotes	clearly
T4823	23271-23277	VBN	denotes	formed
T4826	23277-23279	,	denotes	,
T4827	23279-23282	CC	denotes	and
T4828	23283-23293	NN	denotes	expression
T4830	23294-23296	IN	denotes	of
T4831	23297-23300	DT	denotes	the
T4833	23301-23306	JJ	denotes	early
T4834	23307-23315	NN	denotes	mesoderm
T4835	23316-23322	NN	denotes	marker
T4832	23323-23332	NN	denotes	brachyury
T4836	23333-23334	-LRB-	denotes	(
T4837	23334-23335	NN	denotes	T
T4838	23335-23336	-RRB-	denotes	)
T4839	23337-23338	-LRB-	denotes	[
T4840	23338-23340	CD	denotes	16
T4841	23340-23341	-RRB-	denotes	]
T4842	23342-23345	VBD	denotes	was
T4829	23346-23354	VBN	denotes	detected
T4843	23355-23357	IN	denotes	in
T4844	23358-23361	DT	denotes	the
T4846	23362-23371	JJ	denotes	primitive
T4845	23372-23378	NN	denotes	streak
T4847	23379-23382	CC	denotes	and
T4848	23383-23391	VBG	denotes	emerging
T4849	23392-23401	NN	denotes	notochord
T4850	23402-23404	IN	denotes	by
T4851	23405-23410	JJ	denotes	whole
T4853	23410-23411	HYPH	denotes	-
T4852	23411-23416	NN	denotes	mount
T4855	23417-23419	FW	denotes	in
T4856	23420-23424	FW	denotes	situ
T4854	23425-23438	NN	denotes	hybridisation
T4857	23439-23440	-LRB-	denotes	(
T4858	23440-23445	NN	denotes	WMISH
T4859	23445-23446	-RRB-	denotes	)
T4860	23447-23448	-LRB-	denotes	(
T4862	23448-23454	NN	denotes	Figure
T4861	23455-23457	NN	denotes	5F
T4863	23457-23458	-RRB-	denotes	)
T4864	23458-23460	,	denotes	,
T4865	23460-23470	VBG	denotes	indicating
T4866	23471-23475	IN	denotes	that
T4868	23476-23484	JJ	denotes	Atrxnull
T4869	23485-23492	NNS	denotes	embryos
T4870	23493-23496	VBD	denotes	had
T4867	23497-23508	VBN	denotes	gastrulated
T4871	23508-23509	.	denotes	.
T4872	23509-24621	sentence	denotes	Figure 5 Morphology of Atrxnull Embryos at 7.5 dpc and 8.5 dpc Paraffin sections of wild-type or Atrxnull 7.5 dpc embryos (dissected in their deciduas) were stained with haematoxylin (A) or with an anti-ATRX antibody (H-300, Figure 1) (B–E). Photomicrographs C–E show higher magnification images (200×) of the stained sections shown in (B) (40×). Scale bars represent 200 μm (40× magnification) or 40 μm (200× magnification). a, amnion; ac, amniotic cavity; c, chorion; e, epiblast; ec, ectoplacental cavity; ecc, exocoelomic cavity; ep, ectoplacental cone; ne, neural ectoderm; rm, Reichert's membrane; tgc, trophoblast giant cell. (F) Detection of brachyury (T) expression in Atrxnull 8.5 dpc embryo (head fold stage) by WMISH. The genotype was determined by PCR (as shown in Protocol S1) using DNA extracted from yolk sac. hf, head fold; n, emerging notochord; ps, primitive streak. To investigate whether the reduced size of the Atrxnull embryos was due to an increase in apoptosis, we analysed sections of paraffin-embedded 7.5 dpc embryos by TdT-mediated dUTP nick end labeling (TUNEL) assay (Figure 6A).
T4873	24397-24399	TO	denotes	To
T4874	24400-24411	VB	denotes	investigate
T4876	24412-24419	IN	denotes	whether
T4878	24420-24423	DT	denotes	the
T4880	24424-24431	VBN	denotes	reduced
T4879	24432-24436	NN	denotes	size
T4881	24437-24439	IN	denotes	of
T4882	24440-24443	DT	denotes	the
T4884	24444-24452	JJ	denotes	Atrxnull
T4883	24453-24460	NNS	denotes	embryos
T4877	24461-24464	VBD	denotes	was
T4885	24465-24468	IN	denotes	due
T4886	24469-24471	IN	denotes	to
T4887	24472-24474	DT	denotes	an
T4888	24475-24483	NN	denotes	increase
T4889	24484-24486	IN	denotes	in
T4890	24487-24496	NN	denotes	apoptosis
T4891	24496-24498	,	denotes	,
T4892	24498-24500	PRP	denotes	we
T4875	24501-24509	VBD	denotes	analysed
T4893	24510-24518	NNS	denotes	sections
T4894	24519-24521	IN	denotes	of
T4895	24522-24530	NN	denotes	paraffin
T4897	24530-24531	HYPH	denotes	-
T4896	24531-24539	VBN	denotes	embedded
T4899	24540-24543	CD	denotes	7.5
T4900	24544-24547	NN	denotes	dpc
T4898	24548-24555	NNS	denotes	embryos
T4901	24556-24558	IN	denotes	by
T4902	24559-24562	NN	denotes	TdT
T4904	24562-24563	HYPH	denotes	-
T4903	24563-24571	VBN	denotes	mediated
T4906	24572-24576	NN	denotes	dUTP
T4907	24577-24581	NN	denotes	nick
T4908	24582-24585	NN	denotes	end
T4905	24586-24594	NN	denotes	labeling
T4910	24595-24596	-LRB-	denotes	(
T4911	24596-24601	NN	denotes	TUNEL
T4912	24601-24602	-RRB-	denotes	)
T4909	24603-24608	NN	denotes	assay
T4913	24609-24610	-LRB-	denotes	(
T4915	24610-24616	NN	denotes	Figure
T4914	24617-24619	NN	denotes	6A
T4916	24619-24620	-RRB-	denotes	)
T4917	24620-24621	.	denotes	.
T4918	24621-24690	sentence	denotes	Very few apoptotic cells were detected in wild-type 7.5 dpc embryos.
T4919	24622-24626	RB	denotes	Very
T4920	24627-24630	JJ	denotes	few
T4922	24631-24640	JJ	denotes	apoptotic
T4921	24641-24646	NNS	denotes	cells
T4924	24647-24651	VBD	denotes	were
T4923	24652-24660	VBN	denotes	detected
T4925	24661-24663	IN	denotes	in
T4926	24664-24668	JJ	denotes	wild
T4928	24668-24669	HYPH	denotes	-
T4927	24669-24673	NN	denotes	type
T4930	24674-24677	CD	denotes	7.5
T4931	24678-24681	NN	denotes	dpc
T4929	24682-24689	NNS	denotes	embryos
T4932	24689-24690	.	denotes	.
T4933	24690-24770	sentence	denotes	In Atrxnull embryos, a slight increase in the apoptotic population was evident.
T4934	24691-24693	IN	denotes	In
T4936	24694-24702	JJ	denotes	Atrxnull
T4937	24703-24710	NNS	denotes	embryos
T4938	24710-24712	,	denotes	,
T4939	24712-24713	DT	denotes	a
T4941	24714-24720	JJ	denotes	slight
T4940	24721-24729	NN	denotes	increase
T4942	24730-24732	IN	denotes	in
T4943	24733-24736	DT	denotes	the
T4945	24737-24746	JJ	denotes	apoptotic
T4944	24747-24757	NN	denotes	population
T4935	24758-24761	VBD	denotes	was
T4946	24762-24769	JJ	denotes	evident
T4947	24769-24770	.	denotes	.
T4948	24770-25025	sentence	denotes	However, consistent with our observation of a grossly normal apoptotic index in Atrxnull ES cells (Figure S2), the apoptotic response observed in Atrxnull embryos was also not uniform, but was restricted to a low number of scattered TUNEL-positive cells.
T4949	24771-24778	RB	denotes	However
T4951	24778-24780	,	denotes	,
T4952	24780-24790	JJ	denotes	consistent
T4953	24791-24795	IN	denotes	with
T4954	24796-24799	PRP$	denotes	our
T4955	24800-24811	NN	denotes	observation
T4956	24812-24814	IN	denotes	of
T4957	24815-24816	DT	denotes	a
T4959	24817-24824	RB	denotes	grossly
T4960	24825-24831	JJ	denotes	normal
T4961	24832-24841	JJ	denotes	apoptotic
T4958	24842-24847	NN	denotes	index
T4962	24848-24850	IN	denotes	in
T4963	24851-24859	JJ	denotes	Atrxnull
T4964	24860-24862	NN	denotes	ES
T4965	24863-24868	NNS	denotes	cells
T4966	24869-24870	-LRB-	denotes	(
T4968	24870-24876	NN	denotes	Figure
T4967	24877-24879	NN	denotes	S2
T4969	24879-24880	-RRB-	denotes	)
T4970	24880-24882	,	denotes	,
T4971	24882-24885	DT	denotes	the
T4973	24886-24895	JJ	denotes	apoptotic
T4972	24896-24904	NN	denotes	response
T4974	24905-24913	VBN	denotes	observed
T4975	24914-24916	IN	denotes	in
T4976	24917-24925	JJ	denotes	Atrxnull
T4977	24926-24933	NNS	denotes	embryos
T4950	24934-24937	VBD	denotes	was
T4978	24938-24942	RB	denotes	also
T4979	24943-24946	RB	denotes	not
T4980	24947-24954	JJ	denotes	uniform
T4981	24954-24956	,	denotes	,
T4982	24956-24959	CC	denotes	but
T4983	24960-24963	VBD	denotes	was
T4984	24964-24974	VBN	denotes	restricted
T4985	24975-24977	IN	denotes	to
T4986	24978-24979	DT	denotes	a
T4988	24980-24983	JJ	denotes	low
T4987	24984-24990	NN	denotes	number
T4989	24991-24993	IN	denotes	of
T4990	24994-25003	VBN	denotes	scattered
T4992	25004-25009	NN	denotes	TUNEL
T4994	25009-25010	HYPH	denotes	-
T4993	25010-25018	JJ	denotes	positive
T4991	25019-25024	NNS	denotes	cells
T4995	25024-25025	.	denotes	.
T4996	25025-25326	sentence	denotes	Since this small apoptotic response is unlikely to account fully for the dramatic size deficit observed in Atrxnull embryos, a possible proliferation defect was also investigated by immunohistochemical staining of 7.5 dpc embryo sections for the mitosis marker phosphorylated (Ser10) histone H3 [13].
T4997	25026-25031	IN	denotes	Since
T4999	25032-25036	DT	denotes	this
T5001	25037-25042	JJ	denotes	small
T5002	25043-25052	JJ	denotes	apoptotic
T5000	25053-25061	NN	denotes	response
T4998	25062-25064	VBZ	denotes	is
T5004	25065-25073	JJ	denotes	unlikely
T5005	25074-25076	TO	denotes	to
T5006	25077-25084	VB	denotes	account
T5007	25085-25090	RB	denotes	fully
T5008	25091-25094	IN	denotes	for
T5009	25095-25098	DT	denotes	the
T5011	25099-25107	JJ	denotes	dramatic
T5012	25108-25112	NN	denotes	size
T5010	25113-25120	NN	denotes	deficit
T5013	25121-25129	VBN	denotes	observed
T5014	25130-25132	IN	denotes	in
T5015	25133-25141	JJ	denotes	Atrxnull
T5016	25142-25149	NNS	denotes	embryos
T5017	25149-25151	,	denotes	,
T5018	25151-25152	DT	denotes	a
T5020	25153-25161	JJ	denotes	possible
T5021	25162-25175	NN	denotes	proliferation
T5019	25176-25182	NN	denotes	defect
T5022	25183-25186	VBD	denotes	was
T5023	25187-25191	RB	denotes	also
T5003	25192-25204	VBN	denotes	investigated
T5024	25205-25207	IN	denotes	by
T5025	25208-25227	JJ	denotes	immunohistochemical
T5026	25228-25236	NN	denotes	staining
T5027	25237-25239	IN	denotes	of
T5028	25240-25243	CD	denotes	7.5
T5029	25244-25247	NN	denotes	dpc
T5030	25248-25254	NN	denotes	embryo
T5031	25255-25263	NNS	denotes	sections
T5032	25264-25267	IN	denotes	for
T5033	25268-25271	DT	denotes	the
T5035	25272-25279	NN	denotes	mitosis
T5036	25280-25286	NN	denotes	marker
T5037	25287-25301	VBN	denotes	phosphorylated
T5038	25302-25303	-LRB-	denotes	(
T5039	25303-25308	NN	denotes	Ser10
T5040	25308-25309	-RRB-	denotes	)
T5041	25310-25317	NN	denotes	histone
T5034	25318-25320	NN	denotes	H3
T5042	25321-25322	-LRB-	denotes	[
T5043	25322-25324	CD	denotes	13
T5044	25324-25325	-RRB-	denotes	]
T5045	25325-25326	.	denotes	.
T5046	25326-25507	sentence	denotes	Relative to the very high mitotic index observed in wild-type embryos, the proportion of mitotic cells observed in Atrxnull embryos at 7.5 dpc was dramatically reduced (Figure 6B).
T5047	25327-25335	JJ	denotes	Relative
T5049	25336-25338	IN	denotes	to
T5050	25339-25342	DT	denotes	the
T5052	25343-25347	RB	denotes	very
T5053	25348-25352	JJ	denotes	high
T5054	25353-25360	JJ	denotes	mitotic
T5051	25361-25366	NN	denotes	index
T5055	25367-25375	VBN	denotes	observed
T5056	25376-25378	IN	denotes	in
T5057	25379-25383	JJ	denotes	wild
T5059	25383-25384	HYPH	denotes	-
T5058	25384-25388	NN	denotes	type
T5060	25389-25396	NNS	denotes	embryos
T5061	25396-25398	,	denotes	,
T5062	25398-25401	DT	denotes	the
T5063	25402-25412	NN	denotes	proportion
T5064	25413-25415	IN	denotes	of
T5065	25416-25423	JJ	denotes	mitotic
T5066	25424-25429	NNS	denotes	cells
T5067	25430-25438	VBN	denotes	observed
T5068	25439-25441	IN	denotes	in
T5069	25442-25450	JJ	denotes	Atrxnull
T5070	25451-25458	NNS	denotes	embryos
T5071	25459-25461	IN	denotes	at
T5072	25462-25465	CD	denotes	7.5
T5073	25466-25469	NN	denotes	dpc
T5074	25470-25473	VBD	denotes	was
T5075	25474-25486	RB	denotes	dramatically
T5048	25487-25494	VBN	denotes	reduced
T5076	25495-25496	-LRB-	denotes	(
T5078	25496-25502	NN	denotes	Figure
T5077	25503-25505	NN	denotes	6B
T5079	25505-25506	-RRB-	denotes	)
T5080	25506-25507	.	denotes	.
T5081	25507-25721	sentence	denotes	Taken together, these results suggest that the size deficit observed in Atrxnull embryos prior to lethality largely reflects a proliferative defect, with a minor but indirect contribution from increased apoptosis.
T5082	25508-25513	VBN	denotes	Taken
T5084	25514-25522	RB	denotes	together
T5085	25522-25524	,	denotes	,
T5086	25524-25529	DT	denotes	these
T5087	25530-25537	NNS	denotes	results
T5083	25538-25545	VBP	denotes	suggest
T5088	25546-25550	IN	denotes	that
T5090	25551-25554	DT	denotes	the
T5092	25555-25559	NN	denotes	size
T5091	25560-25567	NN	denotes	deficit
T5093	25568-25576	VBN	denotes	observed
T5094	25577-25579	IN	denotes	in
T5095	25580-25588	JJ	denotes	Atrxnull
T5096	25589-25596	NNS	denotes	embryos
T5097	25597-25602	JJ	denotes	prior
T5098	25603-25605	IN	denotes	to
T5099	25606-25615	NN	denotes	lethality
T5100	25616-25623	RB	denotes	largely
T5089	25624-25632	VBZ	denotes	reflects
T5101	25633-25634	DT	denotes	a
T5103	25635-25648	JJ	denotes	proliferative
T5102	25649-25655	NN	denotes	defect
T5104	25655-25657	,	denotes	,
T5105	25657-25661	IN	denotes	with
T5106	25662-25663	DT	denotes	a
T5108	25664-25669	JJ	denotes	minor
T5109	25670-25673	CC	denotes	but
T5110	25674-25682	JJ	denotes	indirect
T5107	25683-25695	NN	denotes	contribution
T5111	25696-25700	IN	denotes	from
T5112	25701-25710	VBN	denotes	increased
T5113	25711-25720	NN	denotes	apoptosis
T5114	25720-25721	.	denotes	.
T5115	25721-25947	sentence	denotes	Although a growth defect was also observed in Atrxnull ES cells (Figure 3A), in contrast to the Atrxnull embryos, the mitotic index of the ES cell population (as measured with the same antibody) was not depleted (Figure S1B).
T5116	25722-25730	IN	denotes	Although
T5118	25731-25732	DT	denotes	a
T5120	25733-25739	NN	denotes	growth
T5119	25740-25746	NN	denotes	defect
T5121	25747-25750	VBD	denotes	was
T5122	25751-25755	RB	denotes	also
T5117	25756-25764	VBN	denotes	observed
T5124	25765-25767	IN	denotes	in
T5125	25768-25776	JJ	denotes	Atrxnull
T5127	25777-25779	NN	denotes	ES
T5126	25780-25785	NNS	denotes	cells
T5128	25786-25787	-LRB-	denotes	(
T5130	25787-25793	NN	denotes	Figure
T5129	25794-25796	NN	denotes	3A
T5131	25796-25797	-RRB-	denotes	)
T5132	25797-25799	,	denotes	,
T5133	25799-25801	IN	denotes	in
T5134	25802-25810	NN	denotes	contrast
T5135	25811-25813	IN	denotes	to
T5136	25814-25817	DT	denotes	the
T5138	25818-25826	JJ	denotes	Atrxnull
T5137	25827-25834	NNS	denotes	embryos
T5139	25834-25836	,	denotes	,
T5140	25836-25839	DT	denotes	the
T5142	25840-25847	JJ	denotes	mitotic
T5141	25848-25853	NN	denotes	index
T5143	25854-25856	IN	denotes	of
T5144	25857-25860	DT	denotes	the
T5146	25861-25863	NN	denotes	ES
T5147	25864-25868	NN	denotes	cell
T5145	25869-25879	NN	denotes	population
T5148	25880-25881	-LRB-	denotes	(
T5149	25881-25883	IN	denotes	as
T5150	25884-25892	VBN	denotes	measured
T5151	25893-25897	IN	denotes	with
T5152	25898-25901	DT	denotes	the
T5154	25902-25906	JJ	denotes	same
T5153	25907-25915	NN	denotes	antibody
T5155	25915-25916	-RRB-	denotes	)
T5156	25917-25920	VBD	denotes	was
T5157	25921-25924	RB	denotes	not
T5123	25925-25933	VBN	denotes	depleted
T5158	25934-25935	-LRB-	denotes	(
T5160	25935-25941	NN	denotes	Figure
T5159	25942-25945	NN	denotes	S1B
T5161	25945-25946	-RRB-	denotes	)
T5162	25946-25947	.	denotes	.
T5163	25947-26209	sentence	denotes	These observations suggest that the mitotic defect observed in embryos is unlikely to be a direct, cell-autonomous effect of the absence of Atrx, and is more likely to be a secondary effect resulting from the failure to develop a normal trophoblast (see below).
T5164	25948-25953	DT	denotes	These
T5165	25954-25966	NNS	denotes	observations
T5166	25967-25974	VBP	denotes	suggest
T5167	25975-25979	IN	denotes	that
T5169	25980-25983	DT	denotes	the
T5171	25984-25991	JJ	denotes	mitotic
T5170	25992-25998	NN	denotes	defect
T5172	25999-26007	VBN	denotes	observed
T5173	26008-26010	IN	denotes	in
T5174	26011-26018	NNS	denotes	embryos
T5168	26019-26021	VBZ	denotes	is
T5175	26022-26030	JJ	denotes	unlikely
T5176	26031-26033	TO	denotes	to
T5177	26034-26036	VB	denotes	be
T5178	26037-26038	DT	denotes	a
T5180	26039-26045	JJ	denotes	direct
T5181	26045-26047	,	denotes	,
T5182	26047-26051	NN	denotes	cell
T5184	26051-26052	HYPH	denotes	-
T5183	26052-26062	JJ	denotes	autonomous
T5179	26063-26069	NN	denotes	effect
T5185	26070-26072	IN	denotes	of
T5186	26073-26076	DT	denotes	the
T5187	26077-26084	NN	denotes	absence
T5188	26085-26087	IN	denotes	of
T5189	26088-26092	NN	denotes	Atrx
T5190	26092-26094	,	denotes	,
T5191	26094-26097	CC	denotes	and
T5192	26098-26100	VBZ	denotes	is
T5193	26101-26105	RBR	denotes	more
T5194	26106-26112	JJ	denotes	likely
T5195	26113-26115	TO	denotes	to
T5196	26116-26118	VB	denotes	be
T5197	26119-26120	DT	denotes	a
T5199	26121-26130	JJ	denotes	secondary
T5198	26131-26137	NN	denotes	effect
T5200	26138-26147	VBG	denotes	resulting
T5201	26148-26152	IN	denotes	from
T5202	26153-26156	DT	denotes	the
T5203	26157-26164	NN	denotes	failure
T5204	26165-26167	TO	denotes	to
T5205	26168-26175	VB	denotes	develop
T5206	26176-26177	DT	denotes	a
T5208	26178-26184	JJ	denotes	normal
T5207	26185-26196	NN	denotes	trophoblast
T5209	26197-26198	-LRB-	denotes	(
T5210	26198-26201	VB	denotes	see
T5211	26202-26207	RB	denotes	below
T5212	26207-26208	-RRB-	denotes	)
T5213	26208-26209	.	denotes	.
T5495	26877-26890	NN	denotes	Trophectoderm
T5496	26891-26898	NN	denotes	Failure
T5497	26899-26901	IN	denotes	in
T5498	26902-26910	JJ	denotes	Atrxnull
T5499	26911-26918	NNS	denotes	Embryos
T5500	26918-27143	sentence	denotes	Whole-mount observation of 8.5 dpc embryos revealed that, in contrast to the basically normal although delayed morphology of the embryo itself, the extraembryonic tissues of Atrxnull conceptuses appeared highly disorganised.
T5501	26919-26924	JJ	denotes	Whole
T5503	26924-26925	HYPH	denotes	-
T5502	26925-26930	NN	denotes	mount
T5504	26931-26942	NN	denotes	observation
T5506	26943-26945	IN	denotes	of
T5507	26946-26949	CD	denotes	8.5
T5508	26950-26953	NN	denotes	dpc
T5509	26954-26961	NNS	denotes	embryos
T5505	26962-26970	VBD	denotes	revealed
T5510	26971-26975	IN	denotes	that
T5512	26975-26977	,	denotes	,
T5513	26977-26979	IN	denotes	in
T5514	26980-26988	NN	denotes	contrast
T5515	26989-26991	IN	denotes	to
T5516	26992-26995	DT	denotes	the
T5518	26996-27005	RB	denotes	basically
T5519	27006-27012	JJ	denotes	normal
T5520	27013-27021	IN	denotes	although
T5521	27022-27029	VBN	denotes	delayed
T5517	27030-27040	NN	denotes	morphology
T5522	27041-27043	IN	denotes	of
T5523	27044-27047	DT	denotes	the
T5524	27048-27054	NN	denotes	embryo
T5525	27055-27061	PRP	denotes	itself
T5526	27061-27063	,	denotes	,
T5527	27063-27066	DT	denotes	the
T5529	27067-27081	JJ	denotes	extraembryonic
T5528	27082-27089	NNS	denotes	tissues
T5530	27090-27092	IN	denotes	of
T5531	27093-27101	JJ	denotes	Atrxnull
T5532	27102-27113	NNS	denotes	conceptuses
T5511	27114-27122	VBD	denotes	appeared
T5533	27123-27129	RB	denotes	highly
T5534	27130-27142	JJ	denotes	disorganised
T5535	27142-27143	.	denotes	.
T5536	27143-27395	sentence	denotes	When embryos were removed from deciduas, the surrounding trophectoderm layer appeared dramatically reduced in Atrxnull embryos relative to wild-type littermates, and the underlying ectoplacental cone appeared reduced and abnormally shaped (Figure 7A).
T5537	27144-27148	WRB	denotes	When
T5539	27149-27156	NNS	denotes	embryos
T5540	27157-27161	VBD	denotes	were
T5538	27162-27169	VBN	denotes	removed
T5542	27170-27174	IN	denotes	from
T5543	27175-27183	NNS	denotes	deciduas
T5544	27183-27185	,	denotes	,
T5545	27185-27188	DT	denotes	the
T5547	27189-27200	VBG	denotes	surrounding
T5548	27201-27214	NN	denotes	trophectoderm
T5546	27215-27220	NN	denotes	layer
T5541	27221-27229	VBD	denotes	appeared
T5549	27230-27242	RB	denotes	dramatically
T5550	27243-27250	JJ	denotes	reduced
T5551	27251-27253	IN	denotes	in
T5552	27254-27262	JJ	denotes	Atrxnull
T5553	27263-27270	NNS	denotes	embryos
T5554	27271-27279	JJ	denotes	relative
T5555	27280-27282	IN	denotes	to
T5556	27283-27287	JJ	denotes	wild
T5558	27287-27288	HYPH	denotes	-
T5557	27288-27292	NN	denotes	type
T5559	27293-27304	NNS	denotes	littermates
T5560	27304-27306	,	denotes	,
T5561	27306-27309	CC	denotes	and
T5562	27310-27313	DT	denotes	the
T5564	27314-27324	VBG	denotes	underlying
T5565	27325-27338	JJ	denotes	ectoplacental
T5563	27339-27343	NN	denotes	cone
T5566	27344-27352	VBD	denotes	appeared
T5567	27353-27360	JJ	denotes	reduced
T5568	27361-27364	CC	denotes	and
T5569	27365-27375	RB	denotes	abnormally
T5570	27376-27382	VBN	denotes	shaped
T5571	27383-27384	-LRB-	denotes	(
T5573	27384-27390	NN	denotes	Figure
T5572	27391-27393	NN	denotes	7A
T5574	27393-27394	-RRB-	denotes	)
T5575	27394-27395	.	denotes	.
T5576	27395-27590	sentence	denotes	Vacated deciduas surrounding 8.5 dpc wild-type and Atrxnull embryos were bisected and analysed by WMISH for expression of placental lactogen-1 (Pl-1), a marker of terminally differentiated TGCs.
T5577	27396-27403	VBN	denotes	Vacated
T5578	27404-27412	NNS	denotes	deciduas
T5580	27413-27424	VBG	denotes	surrounding
T5581	27425-27428	CD	denotes	8.5
T5582	27429-27432	NN	denotes	dpc
T5584	27433-27437	JJ	denotes	wild
T5586	27437-27438	HYPH	denotes	-
T5585	27438-27442	NN	denotes	type
T5587	27443-27446	CC	denotes	and
T5588	27447-27455	JJ	denotes	Atrxnull
T5583	27456-27463	NNS	denotes	embryos
T5589	27464-27468	VBD	denotes	were
T5579	27469-27477	VBN	denotes	bisected
T5590	27478-27481	CC	denotes	and
T5591	27482-27490	VBN	denotes	analysed
T5592	27491-27493	IN	denotes	by
T5593	27494-27499	NN	denotes	WMISH
T5594	27500-27503	IN	denotes	for
T5595	27504-27514	NN	denotes	expression
T5596	27515-27517	IN	denotes	of
T5597	27518-27527	JJ	denotes	placental
T5598	27528-27536	NN	denotes	lactogen
T5599	27536-27537	HYPH	denotes	-
T5600	27537-27538	CD	denotes	1
T5601	27539-27540	-LRB-	denotes	(
T5602	27540-27542	NN	denotes	Pl
T5603	27542-27543	HYPH	denotes	-
T5604	27543-27544	CD	denotes	1
T5605	27544-27545	-RRB-	denotes	)
T5606	27545-27547	,	denotes	,
T5607	27547-27548	DT	denotes	a
T5608	27549-27555	NN	denotes	marker
T5609	27556-27558	IN	denotes	of
T5610	27559-27569	RB	denotes	terminally
T5611	27570-27584	VBN	denotes	differentiated
T5612	27585-27589	NNS	denotes	TGCs
T5613	27589-27590	.	denotes	.
T5614	27590-27778	sentence	denotes	The number of Pl-1-expressing cells attached to the decidual wall after removal of the embryo is an indication of the density of trophoblast cells surrounding each implantation site [17].
T5615	27591-27594	DT	denotes	The
T5616	27595-27601	NN	denotes	number
T5618	27602-27604	IN	denotes	of
T5619	27605-27607	NN	denotes	Pl
T5621	27607-27608	HYPH	denotes	-
T5622	27608-27609	CD	denotes	1
T5623	27609-27610	HYPH	denotes	-
T5620	27610-27620	VBG	denotes	expressing
T5624	27621-27626	NNS	denotes	cells
T5625	27627-27635	VBN	denotes	attached
T5626	27636-27638	IN	denotes	to
T5627	27639-27642	DT	denotes	the
T5629	27643-27651	JJ	denotes	decidual
T5628	27652-27656	NN	denotes	wall
T5630	27657-27662	IN	denotes	after
T5631	27663-27670	NN	denotes	removal
T5632	27671-27673	IN	denotes	of
T5633	27674-27677	DT	denotes	the
T5634	27678-27684	NN	denotes	embryo
T5617	27685-27687	VBZ	denotes	is
T5635	27688-27690	DT	denotes	an
T5636	27691-27701	NN	denotes	indication
T5637	27702-27704	IN	denotes	of
T5638	27705-27708	DT	denotes	the
T5639	27709-27716	NN	denotes	density
T5640	27717-27719	IN	denotes	of
T5641	27720-27731	NN	denotes	trophoblast
T5642	27732-27737	NNS	denotes	cells
T5643	27738-27749	VBG	denotes	surrounding
T5644	27750-27754	DT	denotes	each
T5646	27755-27767	NN	denotes	implantation
T5645	27768-27772	NN	denotes	site
T5647	27773-27774	-LRB-	denotes	[
T5648	27774-27776	CD	denotes	17
T5649	27776-27777	-RRB-	denotes	]
T5650	27777-27778	.	denotes	.
T5651	27778-28137	sentence	denotes	We found that the population of Pl-1-expressing cells was depleted in the decidual implantation sites containing Atrxnull embryos relative to those containing wild-type littermates (Figure 7B); this was also apparent at 7.5 dpc, as determined by immunohistochemical staining of paraffin sections of embryos in deciduas with an anti-Pl-1 antibody (Figure 7C).
T5652	27779-27781	PRP	denotes	We
T5653	27782-27787	VBD	denotes	found
T5655	27788-27792	IN	denotes	that
T5657	27793-27796	DT	denotes	the
T5658	27797-27807	NN	denotes	population
T5659	27808-27810	IN	denotes	of
T5660	27811-27813	NN	denotes	Pl
T5662	27813-27814	HYPH	denotes	-
T5663	27814-27815	CD	denotes	1
T5664	27815-27816	HYPH	denotes	-
T5661	27816-27826	VBG	denotes	expressing
T5665	27827-27832	NNS	denotes	cells
T5666	27833-27836	VBD	denotes	was
T5656	27837-27845	VBN	denotes	depleted
T5667	27846-27848	IN	denotes	in
T5668	27849-27852	DT	denotes	the
T5670	27853-27861	JJ	denotes	decidual
T5671	27862-27874	NN	denotes	implantation
T5669	27875-27880	NNS	denotes	sites
T5672	27881-27891	VBG	denotes	containing
T5673	27892-27900	JJ	denotes	Atrxnull
T5674	27901-27908	NNS	denotes	embryos
T5675	27909-27917	JJ	denotes	relative
T5676	27918-27920	IN	denotes	to
T5677	27921-27926	DT	denotes	those
T5678	27927-27937	VBG	denotes	containing
T5679	27938-27942	JJ	denotes	wild
T5681	27942-27943	HYPH	denotes	-
T5680	27943-27947	NN	denotes	type
T5682	27948-27959	NNS	denotes	littermates
T5683	27960-27961	-LRB-	denotes	(
T5685	27961-27967	NN	denotes	Figure
T5684	27968-27970	NN	denotes	7B
T5686	27970-27971	-RRB-	denotes	)
T5687	27971-27972	:	denotes	;
T5688	27973-27977	DT	denotes	this
T5654	27978-27981	VBD	denotes	was
T5689	27982-27986	RB	denotes	also
T5690	27987-27995	JJ	denotes	apparent
T5691	27996-27998	IN	denotes	at
T5692	27999-28002	CD	denotes	7.5
T5693	28003-28006	NN	denotes	dpc
T5694	28006-28008	,	denotes	,
T5695	28008-28010	IN	denotes	as
T5696	28011-28021	VBN	denotes	determined
T5697	28022-28024	IN	denotes	by
T5698	28025-28044	JJ	denotes	immunohistochemical
T5699	28045-28053	NN	denotes	staining
T5700	28054-28056	IN	denotes	of
T5701	28057-28065	NN	denotes	paraffin
T5702	28066-28074	NNS	denotes	sections
T5703	28075-28077	IN	denotes	of
T5704	28078-28085	NNS	denotes	embryos
T5705	28086-28088	IN	denotes	in
T5706	28089-28097	NNS	denotes	deciduas
T5707	28098-28102	IN	denotes	with
T5708	28103-28105	DT	denotes	an
T5710	28106-28113	JJ	denotes	anti-Pl
T5712	28113-28114	HYPH	denotes	-
T5711	28114-28115	CD	denotes	1
T5709	28116-28124	NN	denotes	antibody
T5713	28125-28126	-LRB-	denotes	(
T5715	28126-28132	NN	denotes	Figure
T5714	28133-28135	NN	denotes	7C
T5716	28135-28136	-RRB-	denotes	)
T5717	28136-28137	.	denotes	.
T5718	28137-28308	sentence	denotes	A TGC deficiency in the absence of Atrx is consistent with the observation that Atrx is highly expressed in giant cells surrounding wild-type 7.5 dpc embryos (Figure 5E).
T5719	28138-28139	DT	denotes	A
T5721	28140-28143	NN	denotes	TGC
T5720	28144-28154	NN	denotes	deficiency
T5723	28155-28157	IN	denotes	in
T5724	28158-28161	DT	denotes	the
T5725	28162-28169	NN	denotes	absence
T5726	28170-28172	IN	denotes	of
T5727	28173-28177	NN	denotes	Atrx
T5722	28178-28180	VBZ	denotes	is
T5728	28181-28191	JJ	denotes	consistent
T5729	28192-28196	IN	denotes	with
T5730	28197-28200	DT	denotes	the
T5731	28201-28212	NN	denotes	observation
T5732	28213-28217	IN	denotes	that
T5734	28218-28222	NN	denotes	Atrx
T5733	28223-28225	VBZ	denotes	is
T5735	28226-28232	RB	denotes	highly
T5736	28233-28242	JJ	denotes	expressed
T5737	28243-28245	IN	denotes	in
T5738	28246-28251	JJ	denotes	giant
T5739	28252-28257	NNS	denotes	cells
T5740	28258-28269	VBG	denotes	surrounding
T5741	28270-28274	JJ	denotes	wild
T5743	28274-28275	HYPH	denotes	-
T5742	28275-28279	NN	denotes	type
T5745	28280-28283	CD	denotes	7.5
T5746	28284-28287	NN	denotes	dpc
T5744	28288-28295	NNS	denotes	embryos
T5747	28296-28297	-LRB-	denotes	(
T5749	28297-28303	NN	denotes	Figure
T5748	28304-28306	NN	denotes	5E
T5750	28306-28307	-RRB-	denotes	)
T5751	28307-28308	.	denotes	.
T5752	28308-30450	sentence	denotes	Figure 7 Trophectoderm Defect in Atrxnull Embryos (A) 8.5 dpc embryos were dissected from surrounding decidual tissue and observed in whole mount. The genotype of each (indicated above) was determined by PCR using DNA extracted from whole embryos after photography. In the left image, the wild-type female (three-somite stage, left) is surrounded by trophoblast (t) while the trophoblast component surrounding the Atrxnull males (at headfold/presomite [middle] and two-somite stages [right], respectively) is severely depleted. In the right image, the trophoblast has been dissected away from the embryonic region of the wild-type embryo, to reveal the small, abnormally shaped ectoplacental cone (epc) of the mutant littermates. (B) WMISH to detect expression of Pl-1 (a marker of TGCs) at the implantation sites in vacated deciduas that had contained 8.5 dpc wild-type (Atrx WT/WT) or Atrxnull (Atrx Δ18Δneo/Y) embryos. The genotype was determined by PCR using DNA extracted from whole embryos. TGCs are stained with Pl-1. (C) Paraffin sections of wild-type or Atrxnull 7.5 dpc embryos (dissected in their deciduas) were stained with an anti-Pl-1 antibody. The presence or absence of Atrx in each embryo was determined by staining adjacent sections with the anti-ATRX antibody (H-300) as in Figure 5 (unpublished data). (D) Examples of 5-d blastocyst outgrowth cultures. Extensive trophoblast outgrowing from the inner cell mass (icm) was observed in all genotypes. The Atrx genotype and sex of the blastocyst indicated were determined by PCR. To investigate whether the trophoblast defect was restricted to the production of secondary TGCs (produced by diploid precursors in the ectoplacental cone and derived originally from the polar trophectoderm overlying the inner cell mass of the blastocyst) or also affected the production of primary TGCs (resulting from differentiation of the mural trophectoderm of the blastocyst), blastocysts (3.5 dpc) from crosses between Atrx WT/flox females and GATA1-Cre homozygous transgenic males (Atrx WT/Y;GATA1-Cre +/+) were cultured in vitro for 5 d to monitor outgrowth of the primary trophoblast.
T5753	29856-29858	TO	denotes	To
T5754	29859-29870	VB	denotes	investigate
T5756	29871-29878	IN	denotes	whether
T5758	29879-29882	DT	denotes	the
T5760	29883-29894	NN	denotes	trophoblast
T5759	29895-29901	NN	denotes	defect
T5761	29902-29905	VBD	denotes	was
T5757	29906-29916	VBN	denotes	restricted
T5762	29917-29919	IN	denotes	to
T5763	29920-29923	DT	denotes	the
T5764	29924-29934	NN	denotes	production
T5765	29935-29937	IN	denotes	of
T5766	29938-29947	JJ	denotes	secondary
T5767	29948-29952	NNS	denotes	TGCs
T5768	29953-29954	-LRB-	denotes	(
T5769	29954-29962	VBN	denotes	produced
T5770	29963-29965	IN	denotes	by
T5771	29966-29973	NN	denotes	diploid
T5772	29974-29984	NNS	denotes	precursors
T5773	29985-29987	IN	denotes	in
T5774	29988-29991	DT	denotes	the
T5776	29992-30005	JJ	denotes	ectoplacental
T5775	30006-30010	NN	denotes	cone
T5777	30011-30014	CC	denotes	and
T5778	30015-30022	VBN	denotes	derived
T5779	30023-30033	RB	denotes	originally
T5780	30034-30038	IN	denotes	from
T5781	30039-30042	DT	denotes	the
T5783	30043-30048	JJ	denotes	polar
T5782	30049-30062	NN	denotes	trophectoderm
T5784	30063-30072	VBG	denotes	overlying
T5785	30073-30076	DT	denotes	the
T5787	30077-30082	JJ	denotes	inner
T5788	30083-30087	NN	denotes	cell
T5786	30088-30092	NN	denotes	mass
T5789	30093-30095	IN	denotes	of
T5790	30096-30099	DT	denotes	the
T5791	30100-30110	NN	denotes	blastocyst
T5792	30110-30111	-RRB-	denotes	)
T5793	30112-30114	CC	denotes	or
T5794	30115-30119	RB	denotes	also
T5795	30120-30128	VBD	denotes	affected
T5796	30129-30132	DT	denotes	the
T5797	30133-30143	NN	denotes	production
T5798	30144-30146	IN	denotes	of
T5799	30147-30154	JJ	denotes	primary
T5800	30155-30159	NNS	denotes	TGCs
T5801	30160-30161	-LRB-	denotes	(
T5802	30161-30170	VBG	denotes	resulting
T5803	30171-30175	IN	denotes	from
T5804	30176-30191	NN	denotes	differentiation
T5805	30192-30194	IN	denotes	of
T5806	30195-30198	DT	denotes	the
T5808	30199-30204	JJ	denotes	mural
T5807	30205-30218	NN	denotes	trophectoderm
T5809	30219-30221	IN	denotes	of
T5810	30222-30225	DT	denotes	the
T5811	30226-30236	NN	denotes	blastocyst
T5812	30236-30237	-RRB-	denotes	)
T5813	30237-30239	,	denotes	,
T5814	30239-30250	NNS	denotes	blastocysts
T5815	30251-30252	-LRB-	denotes	(
T5817	30252-30255	CD	denotes	3.5
T5816	30256-30259	NN	denotes	dpc
T5818	30259-30260	-RRB-	denotes	)
T5819	30261-30265	IN	denotes	from
T5820	30266-30273	NNS	denotes	crosses
T5821	30274-30281	IN	denotes	between
T5822	30282-30289	NN	denotes	Atrx WT
T5824	30289-30290	HYPH	denotes	/
T5823	30290-30294	NN	denotes	flox
T5825	30295-30302	NNS	denotes	females
T5826	30303-30306	CC	denotes	and
T5827	30307-30312	NN	denotes	GATA1
T5829	30312-30313	HYPH	denotes	-
T5828	30313-30316	NN	denotes	Cre
T5830	30317-30327	JJ	denotes	homozygous
T5832	30328-30338	JJ	denotes	transgenic
T5831	30339-30344	NNS	denotes	males
T5833	30345-30346	-LRB-	denotes	(
T5834	30346-30353	NN	denotes	Atrx WT
T5836	30353-30354	HYPH	denotes	/
T5835	30354-30355	NN	denotes	Y
T5837	30355-30356	:	denotes	;
T5838	30356-30361	NN	denotes	GATA1
T5840	30361-30362	HYPH	denotes	-
T5839	30362-30365	NN	denotes	Cre
T5841	30366-30367	SYM	denotes	+
T5842	30367-30368	HYPH	denotes	/
T5843	30368-30369	SYM	denotes	+
T5844	30369-30370	-RRB-	denotes	)
T5845	30371-30375	VBD	denotes	were
T5755	30376-30384	VBN	denotes	cultured
T5846	30385-30387	FW	denotes	in
T5847	30388-30393	FW	denotes	vitro
T5848	30394-30397	IN	denotes	for
T5849	30398-30399	CD	denotes	5
T5850	30400-30401	NNS	denotes	d
T5851	30402-30404	TO	denotes	to
T5852	30405-30412	VB	denotes	monitor
T5853	30413-30422	NN	denotes	outgrowth
T5854	30423-30425	IN	denotes	of
T5855	30426-30429	DT	denotes	the
T5857	30430-30437	JJ	denotes	primary
T5856	30438-30449	NN	denotes	trophoblast
T5858	30449-30450	.	denotes	.
T5859	30450-30625	sentence	denotes	After 5 d, individual blastocyst cultures were scored for the extent of primary trophoblast outgrowth, and the Atrx genotype and sex of the blastocyst were determined by PCR.
T5860	30451-30456	IN	denotes	After
T5862	30457-30458	CD	denotes	5
T5863	30459-30460	NNS	denotes	d
T5864	30460-30462	,	denotes	,
T5865	30462-30472	JJ	denotes	individual
T5867	30473-30483	NN	denotes	blastocyst
T5866	30484-30492	NNS	denotes	cultures
T5868	30493-30497	VBD	denotes	were
T5861	30498-30504	VBN	denotes	scored
T5869	30505-30508	IN	denotes	for
T5870	30509-30512	DT	denotes	the
T5871	30513-30519	NN	denotes	extent
T5872	30520-30522	IN	denotes	of
T5873	30523-30530	JJ	denotes	primary
T5875	30531-30542	NN	denotes	trophoblast
T5874	30543-30552	NN	denotes	outgrowth
T5876	30552-30554	,	denotes	,
T5877	30554-30557	CC	denotes	and
T5878	30558-30561	DT	denotes	the
T5880	30562-30566	NN	denotes	Atrx
T5879	30567-30575	NN	denotes	genotype
T5882	30576-30579	CC	denotes	and
T5883	30580-30583	NN	denotes	sex
T5884	30584-30586	IN	denotes	of
T5885	30587-30590	DT	denotes	the
T5886	30591-30601	NN	denotes	blastocyst
T5887	30602-30606	VBD	denotes	were
T5881	30607-30617	VBN	denotes	determined
T5888	30618-30620	IN	denotes	by
T5889	30621-30624	NN	denotes	PCR
T5890	30624-30625	.	denotes	.
T5891	30625-30798	sentence	denotes	Most blastocysts hatched from the zona pellucida within 24 h, and trophoblast cells spreading out from the inner cell mass could usually be detected within 48 h of culture.
T5892	30626-30630	JJS	denotes	Most
T5893	30631-30642	NNS	denotes	blastocysts
T5894	30643-30650	VBN	denotes	hatched
T5895	30651-30655	IN	denotes	from
T5896	30656-30659	DT	denotes	the
T5898	30660-30664	NN	denotes	zona
T5897	30665-30674	NN	denotes	pellucida
T5899	30675-30681	IN	denotes	within
T5900	30682-30684	CD	denotes	24
T5901	30685-30686	NNS	denotes	h
T5902	30686-30688	,	denotes	,
T5903	30688-30691	CC	denotes	and
T5904	30692-30703	NN	denotes	trophoblast
T5905	30704-30709	NNS	denotes	cells
T5907	30710-30719	VBG	denotes	spreading
T5908	30720-30723	RP	denotes	out
T5909	30724-30728	IN	denotes	from
T5910	30729-30732	DT	denotes	the
T5912	30733-30738	JJ	denotes	inner
T5913	30739-30743	NN	denotes	cell
T5911	30744-30748	NN	denotes	mass
T5914	30749-30754	MD	denotes	could
T5915	30755-30762	RB	denotes	usually
T5916	30763-30765	VB	denotes	be
T5906	30766-30774	VBN	denotes	detected
T5917	30775-30781	IN	denotes	within
T5918	30782-30784	CD	denotes	48
T5919	30785-30786	NNS	denotes	h
T5920	30787-30789	IN	denotes	of
T5921	30790-30797	NN	denotes	culture
T5922	30797-30798	.	denotes	.
T5923	30798-31161	sentence	denotes	No difference was observed in the rate or extent of trophoblast outgrowth over 5 d of culture between Atrxnull blastocysts (Atrx null/Y, n = 6) and blastocysts bearing an Atrx WT allele (Atrx WT/WT, n = 6; Atrx WT/null, n = 6; Atrx WT/Y, n = 6) (examples shown in Figure 7D), suggesting that the defect specifically involves the secondary giant cell compartment.
T5924	30799-30801	DT	denotes	No
T5925	30802-30812	NN	denotes	difference
T5927	30813-30816	VBD	denotes	was
T5926	30817-30825	VBN	denotes	observed
T5928	30826-30828	IN	denotes	in
T5929	30829-30832	DT	denotes	the
T5930	30833-30837	NN	denotes	rate
T5931	30838-30840	CC	denotes	or
T5932	30841-30847	NN	denotes	extent
T5933	30848-30850	IN	denotes	of
T5934	30851-30862	NN	denotes	trophoblast
T5935	30863-30872	NN	denotes	outgrowth
T5936	30873-30877	IN	denotes	over
T5937	30878-30879	CD	denotes	5
T5938	30880-30881	NNS	denotes	d
T5939	30882-30884	IN	denotes	of
T5940	30885-30892	NN	denotes	culture
T5941	30893-30900	IN	denotes	between
T5942	30901-30909	JJ	denotes	Atrxnull
T5943	30910-30921	NNS	denotes	blastocysts
T5944	30922-30923	-LRB-	denotes	(
T5946	30923-30932	JJ	denotes	Atrx null
T5947	30932-30933	HYPH	denotes	/
T5945	30933-30934	NN	denotes	Y
T5948	30934-30936	,	denotes	,
T5949	30936-30937	NN	denotes	n
T5951	30938-30939	SYM	denotes	=
T5950	30940-30941	CD	denotes	6
T5952	30941-30942	-RRB-	denotes	)
T5953	30943-30946	CC	denotes	and
T5954	30947-30958	NNS	denotes	blastocysts
T5955	30959-30966	VBG	denotes	bearing
T5956	30967-30969	DT	denotes	an
T5958	30970-30977	NN	denotes	Atrx WT
T5957	30978-30984	NN	denotes	allele
T5959	30985-30986	-LRB-	denotes	(
T5961	30986-30993	NN	denotes	Atrx WT
T5963	30993-30994	HYPH	denotes	/
T5962	30994-30996	NN	denotes	WT
T5965	30996-30998	,	denotes	,
T5966	30998-30999	NN	denotes	n
T5967	31000-31001	SYM	denotes	=
T5964	31002-31003	CD	denotes	6
T5968	31003-31004	:	denotes	;
T5969	31005-31012	NN	denotes	Atrx WT
T5971	31012-31013	HYPH	denotes	/
T5970	31013-31017	NN	denotes	null
T5973	31017-31019	,	denotes	,
T5974	31019-31020	NN	denotes	n
T5975	31021-31022	SYM	denotes	=
T5972	31023-31024	CD	denotes	6
T5976	31024-31025	:	denotes	;
T5977	31026-31033	NN	denotes	Atrx WT
T5979	31033-31034	HYPH	denotes	/
T5978	31034-31035	NN	denotes	Y
T5980	31035-31037	,	denotes	,
T5981	31037-31038	NN	denotes	n
T5982	31039-31040	SYM	denotes	=
T5960	31041-31042	CD	denotes	6
T5983	31042-31043	-RRB-	denotes	)
T5984	31044-31045	-LRB-	denotes	(
T5986	31045-31053	NNS	denotes	examples
T5985	31054-31059	VBN	denotes	shown
T5987	31060-31062	IN	denotes	in
T5988	31063-31069	NN	denotes	Figure
T5989	31070-31072	NN	denotes	7D
T5990	31072-31073	-RRB-	denotes	)
T5991	31073-31075	,	denotes	,
T5992	31075-31085	VBG	denotes	suggesting
T5993	31086-31090	IN	denotes	that
T5995	31091-31094	DT	denotes	the
T5996	31095-31101	NN	denotes	defect
T5997	31102-31114	RB	denotes	specifically
T5994	31115-31123	VBZ	denotes	involves
T5998	31124-31127	DT	denotes	the
T6000	31128-31137	JJ	denotes	secondary
T6001	31138-31143	JJ	denotes	giant
T6002	31144-31148	NN	denotes	cell
T5999	31149-31160	NN	denotes	compartment
T6003	31160-31161	.	denotes	.
T6004	31161-31277	sentence	denotes	This is consistent with the observation that Atrxnull conceptuses implant successfully and survive to gastrulation.
T6005	31162-31166	DT	denotes	This
T6006	31167-31169	VBZ	denotes	is
T6007	31170-31180	JJ	denotes	consistent
T6008	31181-31185	IN	denotes	with
T6009	31186-31189	DT	denotes	the
T6010	31190-31201	NN	denotes	observation
T6011	31202-31206	IN	denotes	that
T6013	31207-31215	JJ	denotes	Atrxnull
T6014	31216-31227	NNS	denotes	conceptuses
T6012	31228-31235	VBP	denotes	implant
T6015	31236-31248	RB	denotes	successfully
T6016	31249-31252	CC	denotes	and
T6017	31253-31260	VBP	denotes	survive
T6018	31261-31263	IN	denotes	to
T6019	31264-31276	NN	denotes	gastrulation
T6020	31276-31277	.	denotes	.
T6021	31277-31423	sentence	denotes	Taken together, these data suggest that loss of Atrx results in a defect in formation of the secondary trophoblast that is apparent from 7.5 dpc.
T6022	31278-31283	VBN	denotes	Taken
T6024	31284-31292	RB	denotes	together
T6025	31292-31294	,	denotes	,
T6026	31294-31299	DT	denotes	these
T6027	31300-31304	NNS	denotes	data
T6023	31305-31312	VBP	denotes	suggest
T6028	31313-31317	IN	denotes	that
T6030	31318-31322	NN	denotes	loss
T6031	31323-31325	IN	denotes	of
T6032	31326-31330	NN	denotes	Atrx
T6029	31331-31338	VBZ	denotes	results
T6033	31339-31341	IN	denotes	in
T6034	31342-31343	DT	denotes	a
T6035	31344-31350	NN	denotes	defect
T6036	31351-31353	IN	denotes	in
T6037	31354-31363	NN	denotes	formation
T6038	31364-31366	IN	denotes	of
T6039	31367-31370	DT	denotes	the
T6041	31371-31380	JJ	denotes	secondary
T6040	31381-31392	NN	denotes	trophoblast
T6042	31393-31397	WDT	denotes	that
T6043	31398-31400	VBZ	denotes	is
T6044	31401-31409	JJ	denotes	apparent
T6045	31410-31414	IN	denotes	from
T6046	31415-31418	CD	denotes	7.5
T6047	31419-31422	NN	denotes	dpc
T6048	31422-31423	.	denotes	.
T6049	31423-31668	sentence	denotes	Despite initiating normal organisation in the embryo proper, Atrxnull conceptuses exhibit a proliferative defect by 7.5 dpc and die by around 9.5 dpc, probably due to a nutritional deficit resulting from failure to develop a normal trophoblast.
T6050	31424-31431	IN	denotes	Despite
T6052	31432-31442	VBG	denotes	initiating
T6053	31443-31449	JJ	denotes	normal
T6054	31450-31462	NN	denotes	organisation
T6055	31463-31465	IN	denotes	in
T6056	31466-31469	DT	denotes	the
T6057	31470-31476	NN	denotes	embryo
T6058	31477-31483	JJ	denotes	proper
T6059	31483-31485	,	denotes	,
T6060	31485-31493	JJ	denotes	Atrxnull
T6061	31494-31505	NNS	denotes	conceptuses
T6051	31506-31513	VBP	denotes	exhibit
T6062	31514-31515	DT	denotes	a
T6064	31516-31529	JJ	denotes	proliferative
T6063	31530-31536	NN	denotes	defect
T6065	31537-31539	IN	denotes	by
T6066	31540-31543	CD	denotes	7.5
T6067	31544-31547	NN	denotes	dpc
T6068	31548-31551	CC	denotes	and
T6069	31552-31555	VBP	denotes	die
T6070	31556-31558	IN	denotes	by
T6071	31559-31565	IN	denotes	around
T6072	31566-31569	CD	denotes	9.5
T6073	31570-31573	NN	denotes	dpc
T6074	31573-31575	,	denotes	,
T6075	31575-31583	RB	denotes	probably
T6076	31584-31587	IN	denotes	due
T6077	31588-31590	IN	denotes	to
T6078	31591-31592	DT	denotes	a
T6080	31593-31604	JJ	denotes	nutritional
T6079	31605-31612	NN	denotes	deficit
T6081	31613-31622	VBG	denotes	resulting
T6082	31623-31627	IN	denotes	from
T6083	31628-31635	NN	denotes	failure
T6084	31636-31638	TO	denotes	to
T6085	31639-31646	VB	denotes	develop
T6086	31647-31648	DT	denotes	a
T6088	31649-31655	JJ	denotes	normal
T6087	31656-31667	NN	denotes	trophoblast
T6089	31667-31668	.	denotes	.
T6480	31670-31676	NN	denotes	Escape
T6481	31677-31681	IN	denotes	from
T6482	31682-31691	VBN	denotes	Imprinted
T6483	31692-31704	NN	denotes	Inactivation
T6484	31705-31707	IN	denotes	of
T6485	31708-31711	DT	denotes	the
T6487	31712-31722	RB	denotes	Paternally
T6488	31723-31732	VBN	denotes	Inherited
T6489	31733-31740	NN	denotes	Atrx WT
T6486	31741-31747	NN	denotes	Allele
T6490	31748-31750	IN	denotes	in
T6491	31751-31765	JJ	denotes	Extraembryonic
T6492	31766-31773	NNS	denotes	Tissues
T6493	31774-31776	IN	denotes	of
T6494	31777-31784	NN	denotes	Carrier
T6496	31785-31791	JJ	denotes	Female
T6495	31792-31796	NNS	denotes	Mice
T6497	31796-32142	sentence	denotes	Female mice carrying an Atrx null allele (Atrx WT/null;GATA1-cre +/−) were detected at 9.5 dpc (Table 1) and recovered at birth (unpublished data), although at both time points the number of carrier females was lower than that of wild-type (Atrx WT/WT;GATA1-cre +/−) females, suggesting that a proportion of carrier female embryos died in utero.
T6498	31797-31803	JJ	denotes	Female
T6499	31804-31808	NNS	denotes	mice
T6501	31809-31817	VBG	denotes	carrying
T6502	31818-31820	DT	denotes	an
T6504	31821-31830	JJ	denotes	Atrx null
T6503	31831-31837	NN	denotes	allele
T6505	31838-31839	-LRB-	denotes	(
T6507	31839-31846	NN	denotes	Atrx WT
T6509	31846-31847	HYPH	denotes	/
T6508	31847-31851	NN	denotes	null
T6510	31851-31852	:	denotes	;
T6511	31852-31857	NN	denotes	GATA1
T6512	31857-31858	HYPH	denotes	-
T6506	31858-31861	NN	denotes	cre
T6513	31862-31863	SYM	denotes	+
T6514	31863-31864	HYPH	denotes	/
T6515	31864-31865	SYM	denotes	−
T6516	31865-31866	-RRB-	denotes	)
T6517	31867-31871	VBD	denotes	were
T6500	31872-31880	VBN	denotes	detected
T6518	31881-31883	IN	denotes	at
T6519	31884-31887	CD	denotes	9.5
T6520	31888-31891	NN	denotes	dpc
T6521	31892-31893	-LRB-	denotes	(
T6522	31893-31898	NN	denotes	Table
T6523	31899-31900	CD	denotes	1
T6524	31900-31901	-RRB-	denotes	)
T6525	31902-31905	CC	denotes	and
T6526	31906-31915	VBN	denotes	recovered
T6527	31916-31918	IN	denotes	at
T6528	31919-31924	NN	denotes	birth
T6529	31925-31926	-LRB-	denotes	(
T6531	31926-31937	JJ	denotes	unpublished
T6530	31938-31942	NNS	denotes	data
T6532	31942-31943	-RRB-	denotes	)
T6533	31943-31945	,	denotes	,
T6534	31945-31953	IN	denotes	although
T6536	31954-31956	IN	denotes	at
T6537	31957-31961	CC	denotes	both
T6539	31962-31966	NN	denotes	time
T6538	31967-31973	NNS	denotes	points
T6540	31974-31977	DT	denotes	the
T6541	31978-31984	NN	denotes	number
T6542	31985-31987	IN	denotes	of
T6543	31988-31995	NN	denotes	carrier
T6544	31996-32003	NNS	denotes	females
T6535	32004-32007	VBD	denotes	was
T6545	32008-32013	JJR	denotes	lower
T6546	32014-32018	IN	denotes	than
T6547	32019-32023	DT	denotes	that
T6548	32024-32026	IN	denotes	of
T6549	32027-32031	JJ	denotes	wild
T6551	32031-32032	HYPH	denotes	-
T6550	32032-32036	NN	denotes	type
T6553	32037-32038	-LRB-	denotes	(
T6555	32038-32045	NN	denotes	Atrx WT
T6557	32045-32046	HYPH	denotes	/
T6556	32046-32048	NN	denotes	WT
T6558	32048-32049	:	denotes	;
T6559	32049-32054	NN	denotes	GATA1
T6560	32054-32055	HYPH	denotes	-
T6554	32055-32058	NN	denotes	cre
T6561	32059-32060	SYM	denotes	+
T6562	32060-32061	HYPH	denotes	/
T6563	32061-32062	SYM	denotes	−
T6564	32062-32063	-RRB-	denotes	)
T6552	32064-32071	NNS	denotes	females
T6565	32071-32073	,	denotes	,
T6566	32073-32083	VBG	denotes	suggesting
T6567	32084-32088	IN	denotes	that
T6569	32089-32090	DT	denotes	a
T6570	32091-32101	NN	denotes	proportion
T6571	32102-32104	IN	denotes	of
T6572	32105-32112	NN	denotes	carrier
T6574	32113-32119	JJ	denotes	female
T6573	32120-32127	NNS	denotes	embryos
T6568	32128-32132	VBD	denotes	died
T6575	32133-32135	FW	denotes	in
T6576	32136-32141	FW	denotes	utero
T6577	32141-32142	.	denotes	.
T6578	32142-32286	sentence	denotes	Surviving adult carrier female mice were not phenotypically normal and exhibited mild behavioural abnormalities, although some could reproduce.
T6579	32143-32152	VBG	denotes	Surviving
T6581	32153-32158	JJ	denotes	adult
T6582	32159-32166	NN	denotes	carrier
T6583	32167-32173	JJ	denotes	female
T6580	32174-32178	NNS	denotes	mice
T6584	32179-32183	VBD	denotes	were
T6585	32184-32187	RB	denotes	not
T6586	32188-32202	RB	denotes	phenotypically
T6587	32203-32209	JJ	denotes	normal
T6588	32210-32213	CC	denotes	and
T6589	32214-32223	VBD	denotes	exhibited
T6590	32224-32228	JJ	denotes	mild
T6592	32229-32240	JJ	denotes	behavioural
T6591	32241-32254	NNS	denotes	abnormalities
T6593	32254-32256	,	denotes	,
T6594	32256-32264	IN	denotes	although
T6596	32265-32269	DT	denotes	some
T6597	32270-32275	MD	denotes	could
T6595	32276-32285	VB	denotes	reproduce
T6598	32285-32286	.	denotes	.
T6599	32286-32446	sentence	denotes	For all Atrx WT/null carrier female embryos presented in Table 1, the Atrx WT allele was paternally derived, while the Atrx null allele was maternally derived.
T6600	32287-32290	IN	denotes	For
T6602	32291-32294	DT	denotes	all
T6604	32295-32302	NN	denotes	Atrx WT
T6606	32302-32303	HYPH	denotes	/
T6605	32303-32307	JJ	denotes	null
T6607	32308-32315	NN	denotes	carrier
T6608	32316-32322	JJ	denotes	female
T6603	32323-32330	NNS	denotes	embryos
T6609	32331-32340	VBN	denotes	presented
T6610	32341-32343	IN	denotes	in
T6611	32344-32349	NN	denotes	Table
T6612	32350-32351	CD	denotes	1
T6613	32351-32353	,	denotes	,
T6614	32353-32356	DT	denotes	the
T6616	32357-32364	NN	denotes	Atrx WT
T6615	32365-32371	NN	denotes	allele
T6617	32372-32375	VBD	denotes	was
T6618	32376-32386	RB	denotes	paternally
T6601	32387-32394	VBN	denotes	derived
T6619	32394-32396	,	denotes	,
T6620	32396-32401	IN	denotes	while
T6622	32402-32405	DT	denotes	the
T6624	32406-32415	JJ	denotes	Atrx null
T6623	32416-32422	NN	denotes	allele
T6625	32423-32426	VBD	denotes	was
T6626	32427-32437	RB	denotes	maternally
T6621	32438-32445	VBN	denotes	derived
T6627	32445-32446	.	denotes	.
T6628	32446-32685	sentence	denotes	In the mouse, X chromosome inactivation is subject to parental imprinting in the trophectoderm and primitive endoderm lineages that give rise to the extraembryonic tissues, resulting in inactivation of the paternal X chromosome (Xp) [18].
T6629	32447-32449	IN	denotes	In
T6631	32450-32453	DT	denotes	the
T6632	32454-32459	NN	denotes	mouse
T6633	32459-32461	,	denotes	,
T6634	32461-32462	NN	denotes	X
T6635	32463-32473	NN	denotes	chromosome
T6636	32474-32486	NN	denotes	inactivation
T6630	32487-32489	VBZ	denotes	is
T6637	32490-32497	JJ	denotes	subject
T6638	32498-32500	IN	denotes	to
T6639	32501-32509	JJ	denotes	parental
T6640	32510-32520	NN	denotes	imprinting
T6641	32521-32523	IN	denotes	in
T6642	32524-32527	DT	denotes	the
T6643	32528-32541	NN	denotes	trophectoderm
T6644	32542-32545	CC	denotes	and
T6645	32546-32555	JJ	denotes	primitive
T6647	32556-32564	NN	denotes	endoderm
T6646	32565-32573	NNS	denotes	lineages
T6648	32574-32578	WDT	denotes	that
T6649	32579-32583	VBP	denotes	give
T6650	32584-32588	NN	denotes	rise
T6651	32589-32591	IN	denotes	to
T6652	32592-32595	DT	denotes	the
T6654	32596-32610	JJ	denotes	extraembryonic
T6653	32611-32618	NNS	denotes	tissues
T6655	32618-32620	,	denotes	,
T6656	32620-32629	VBG	denotes	resulting
T6657	32630-32632	IN	denotes	in
T6658	32633-32645	NN	denotes	inactivation
T6659	32646-32648	IN	denotes	of
T6660	32649-32652	DT	denotes	the
T6662	32653-32661	JJ	denotes	paternal
T6663	32662-32663	NN	denotes	X
T6661	32664-32674	NN	denotes	chromosome
T6664	32675-32676	-LRB-	denotes	(
T6665	32676-32678	NN	denotes	Xp
T6666	32678-32679	-RRB-	denotes	)
T6667	32680-32681	-LRB-	denotes	[
T6668	32681-32683	CD	denotes	18
T6669	32683-32684	-RRB-	denotes	]
T6670	32684-32685	.	denotes	.
T6671	32685-32814	sentence	denotes	In contrast, in tissues of the embryo proper (derived from the inner cell mass of the blastocyst) X-inactivation is random [19].
T6672	32686-32688	IN	denotes	In
T6674	32689-32697	NN	denotes	contrast
T6675	32697-32699	,	denotes	,
T6676	32699-32701	IN	denotes	in
T6677	32702-32709	NNS	denotes	tissues
T6678	32710-32712	IN	denotes	of
T6679	32713-32716	DT	denotes	the
T6680	32717-32723	NN	denotes	embryo
T6681	32724-32730	JJ	denotes	proper
T6682	32731-32732	-LRB-	denotes	(
T6683	32732-32739	VBN	denotes	derived
T6684	32740-32744	IN	denotes	from
T6685	32745-32748	DT	denotes	the
T6687	32749-32754	JJ	denotes	inner
T6688	32755-32759	NN	denotes	cell
T6686	32760-32764	NN	denotes	mass
T6689	32765-32767	IN	denotes	of
T6690	32768-32771	DT	denotes	the
T6691	32772-32782	NN	denotes	blastocyst
T6692	32782-32783	-RRB-	denotes	)
T6693	32784-32785	NN	denotes	X
T6695	32785-32786	HYPH	denotes	-
T6694	32786-32798	NN	denotes	inactivation
T6673	32799-32801	VBZ	denotes	is
T6696	32802-32808	JJ	denotes	random
T6697	32809-32810	-LRB-	denotes	[
T6698	32810-32812	CD	denotes	19
T6699	32812-32813	-RRB-	denotes	]
T6700	32813-32814	.	denotes	.
T6701	32814-33147	sentence	denotes	Thus, in the extraembryonic compartment of Atrx WT/null carrier females, normal imprinted X-inactivation would be expected to result in silencing of the paternally derived Atrx WT allele, leaving only the Atrx null allele on the active maternal X (Xm) and thereby render the extraembryonic tissues null for full-length Atrx protein.
T6702	32815-32819	RB	denotes	Thus
T6704	32819-32821	,	denotes	,
T6705	32821-32823	IN	denotes	in
T6706	32824-32827	DT	denotes	the
T6708	32828-32842	JJ	denotes	extraembryonic
T6707	32843-32854	NN	denotes	compartment
T6709	32855-32857	IN	denotes	of
T6710	32858-32865	NN	denotes	Atrx WT
T6712	32865-32866	HYPH	denotes	/
T6711	32866-32870	JJ	denotes	null
T6714	32871-32878	NN	denotes	carrier
T6713	32879-32886	NNS	denotes	females
T6715	32886-32888	,	denotes	,
T6716	32888-32894	JJ	denotes	normal
T6718	32895-32904	VBN	denotes	imprinted
T6719	32905-32906	NN	denotes	X
T6720	32906-32907	HYPH	denotes	-
T6717	32907-32919	NN	denotes	inactivation
T6721	32920-32925	MD	denotes	would
T6722	32926-32928	VB	denotes	be
T6703	32929-32937	VBN	denotes	expected
T6723	32938-32940	TO	denotes	to
T6724	32941-32947	VB	denotes	result
T6725	32948-32950	IN	denotes	in
T6726	32951-32960	VBG	denotes	silencing
T6727	32961-32963	IN	denotes	of
T6728	32964-32967	DT	denotes	the
T6730	32968-32978	RB	denotes	paternally
T6731	32979-32986	VBN	denotes	derived
T6732	32987-32994	NN	denotes	Atrx WT
T6729	32995-33001	NN	denotes	allele
T6733	33001-33003	,	denotes	,
T6734	33003-33010	VBG	denotes	leaving
T6735	33011-33015	RB	denotes	only
T6737	33016-33019	DT	denotes	the
T6738	33020-33029	JJ	denotes	Atrx null
T6736	33030-33036	NN	denotes	allele
T6739	33037-33039	IN	denotes	on
T6740	33040-33043	DT	denotes	the
T6742	33044-33050	JJ	denotes	active
T6743	33051-33059	JJ	denotes	maternal
T6741	33060-33061	NN	denotes	X
T6744	33062-33063	-LRB-	denotes	(
T6745	33063-33065	NN	denotes	Xm
T6746	33065-33066	-RRB-	denotes	)
T6747	33067-33070	CC	denotes	and
T6748	33071-33078	RB	denotes	thereby
T6749	33079-33085	VB	denotes	render
T6750	33086-33089	DT	denotes	the
T6752	33090-33104	JJ	denotes	extraembryonic
T6751	33105-33112	NNS	denotes	tissues
T6753	33113-33117	JJ	denotes	null
T6754	33118-33121	IN	denotes	for
T6755	33122-33126	JJ	denotes	full
T6757	33126-33127	HYPH	denotes	-
T6756	33127-33133	NN	denotes	length
T6759	33134-33138	NN	denotes	Atrx
T6758	33139-33146	NN	denotes	protein
T6760	33146-33147	.	denotes	.
T6761	33147-33242	sentence	denotes	However, the absence of Atrx in the extraembryonic compartment is lethal in Atrx null/Y males.
T6762	33148-33155	RB	denotes	However
T6764	33155-33157	,	denotes	,
T6765	33157-33160	DT	denotes	the
T6766	33161-33168	NN	denotes	absence
T6767	33169-33171	IN	denotes	of
T6768	33172-33176	NN	denotes	Atrx
T6769	33177-33179	IN	denotes	in
T6770	33180-33183	DT	denotes	the
T6772	33184-33198	JJ	denotes	extraembryonic
T6771	33199-33210	NN	denotes	compartment
T6763	33211-33213	VBZ	denotes	is
T6773	33214-33220	JJ	denotes	lethal
T6774	33221-33223	IN	denotes	in
T6775	33224-33233	JJ	denotes	Atrx null
T6777	33233-33234	HYPH	denotes	/
T6776	33234-33235	NN	denotes	Y
T6778	33236-33241	NNS	denotes	males
T6779	33241-33242	.	denotes	.
T6780	33242-33432	sentence	denotes	This suggested the possibility of an escape from imprinted inactivation of the paternally derived Atrx WT allele in the extraembryonic compartment of a proportion of carrier female embryos.
T6781	33243-33247	DT	denotes	This
T6782	33248-33257	VBD	denotes	suggested
T6783	33258-33261	DT	denotes	the
T6784	33262-33273	NN	denotes	possibility
T6785	33274-33276	IN	denotes	of
T6786	33277-33279	DT	denotes	an
T6787	33280-33286	NN	denotes	escape
T6788	33287-33291	IN	denotes	from
T6789	33292-33301	VBN	denotes	imprinted
T6790	33302-33314	NN	denotes	inactivation
T6791	33315-33317	IN	denotes	of
T6792	33318-33321	DT	denotes	the
T6794	33322-33332	RB	denotes	paternally
T6795	33333-33340	VBN	denotes	derived
T6796	33341-33348	NN	denotes	Atrx WT
T6793	33349-33355	NN	denotes	allele
T6797	33356-33358	IN	denotes	in
T6798	33359-33362	DT	denotes	the
T6800	33363-33377	JJ	denotes	extraembryonic
T6799	33378-33389	NN	denotes	compartment
T6801	33390-33392	IN	denotes	of
T6802	33393-33394	DT	denotes	a
T6803	33395-33405	NN	denotes	proportion
T6804	33406-33408	IN	denotes	of
T6805	33409-33416	NN	denotes	carrier
T6807	33417-33423	JJ	denotes	female
T6806	33424-33431	NNS	denotes	embryos
T6808	33431-33432	.	denotes	.
T6809	33432-33700	sentence	denotes	To investigate further, we crossed homozygous Atrx flox/flox females and homozygous GATA1-cre transgenic males (Atrx WT/Y;GATA1-cre +/+), which would be expected to yield only Atrxnull males (Atrxnull/Y;GATA1-cre +/−) and carrier females (Atrx WT/null;GATA1-cre +/−).
T6810	33433-33435	TO	denotes	To
T6811	33436-33447	VB	denotes	investigate
T6813	33448-33455	RB	denotes	further
T6814	33455-33457	,	denotes	,
T6815	33457-33459	PRP	denotes	we
T6812	33460-33467	VBD	denotes	crossed
T6816	33468-33478	JJ	denotes	homozygous
T6818	33479-33488	NN	denotes	Atrx flox
T6820	33488-33489	HYPH	denotes	/
T6819	33489-33493	NN	denotes	flox
T6817	33494-33501	NNS	denotes	females
T6821	33502-33505	CC	denotes	and
T6822	33506-33516	JJ	denotes	homozygous
T6824	33517-33522	NN	denotes	GATA1
T6826	33522-33523	HYPH	denotes	-
T6825	33523-33526	NN	denotes	cre
T6827	33527-33537	JJ	denotes	transgenic
T6823	33538-33543	NNS	denotes	males
T6828	33544-33545	-LRB-	denotes	(
T6830	33545-33552	NN	denotes	Atrx WT
T6832	33552-33553	HYPH	denotes	/
T6831	33553-33554	NN	denotes	Y
T6833	33554-33555	:	denotes	;
T6834	33555-33560	NN	denotes	GATA1
T6835	33560-33561	HYPH	denotes	-
T6829	33561-33564	NN	denotes	cre
T6836	33565-33566	SYM	denotes	+
T6837	33566-33567	HYPH	denotes	/
T6838	33567-33568	SYM	denotes	+
T6839	33568-33569	-RRB-	denotes	)
T6840	33569-33571	,	denotes	,
T6841	33571-33576	WDT	denotes	which
T6843	33577-33582	MD	denotes	would
T6844	33583-33585	VB	denotes	be
T6842	33586-33594	VBN	denotes	expected
T6845	33595-33597	TO	denotes	to
T6846	33598-33603	VB	denotes	yield
T6847	33604-33608	RB	denotes	only
T6849	33609-33617	JJ	denotes	Atrxnull
T6848	33618-33623	NNS	denotes	males
T6850	33624-33625	-LRB-	denotes	(
T6852	33625-33633	JJ	denotes	Atrxnull
T6854	33633-33634	HYPH	denotes	/
T6853	33634-33635	NN	denotes	Y
T6855	33635-33636	:	denotes	;
T6856	33636-33641	NN	denotes	GATA1
T6857	33641-33642	HYPH	denotes	-
T6851	33642-33645	NN	denotes	cre
T6858	33646-33647	SYM	denotes	+
T6859	33647-33648	HYPH	denotes	/
T6860	33648-33649	SYM	denotes	−
T6861	33649-33650	-RRB-	denotes	)
T6862	33651-33654	CC	denotes	and
T6863	33655-33662	NN	denotes	carrier
T6864	33663-33670	NNS	denotes	females
T6865	33671-33672	-LRB-	denotes	(
T6867	33672-33679	NN	denotes	Atrx WT
T6869	33679-33680	HYPH	denotes	/
T6868	33680-33684	NN	denotes	null
T6870	33684-33685	:	denotes	;
T6871	33685-33690	NN	denotes	GATA1
T6872	33690-33691	HYPH	denotes	-
T6866	33691-33694	NN	denotes	cre
T6873	33695-33696	SYM	denotes	+
T6874	33696-33697	HYPH	denotes	/
T6875	33697-33698	SYM	denotes	−
T6876	33698-33699	-RRB-	denotes	)
T6877	33699-33700	.	denotes	.
T6878	33700-33770	sentence	denotes	In these carrier females, the Atrx WT allele is paternally inherited.
T6879	33701-33703	IN	denotes	In
T6881	33704-33709	DT	denotes	these
T6883	33710-33717	NN	denotes	carrier
T6882	33718-33725	NNS	denotes	females
T6884	33725-33727	,	denotes	,
T6885	33727-33730	DT	denotes	the
T6887	33731-33738	NN	denotes	Atrx WT
T6886	33739-33745	NN	denotes	allele
T6888	33746-33748	VBZ	denotes	is
T6889	33749-33759	RB	denotes	paternally
T6880	33760-33769	VBN	denotes	inherited
T6890	33769-33770	.	denotes	.
T6891	33770-33962	sentence	denotes	Embryos were dissected in their deciduas at 7.5 dpc, and paraffin sections were stained with anti-ATRX antibody, along with sections from a wild-type 7.5 dpc embryo for comparison (Figure 8).
T6892	33771-33778	NNS	denotes	Embryos
T6894	33779-33783	VBD	denotes	were
T6893	33784-33793	VBN	denotes	dissected
T6895	33794-33796	IN	denotes	in
T6896	33797-33802	PRP$	denotes	their
T6897	33803-33811	NNS	denotes	deciduas
T6898	33812-33814	IN	denotes	at
T6899	33815-33818	CD	denotes	7.5
T6900	33819-33822	NN	denotes	dpc
T6901	33822-33824	,	denotes	,
T6902	33824-33827	CC	denotes	and
T6903	33828-33836	NN	denotes	paraffin
T6904	33837-33845	NNS	denotes	sections
T6906	33846-33850	VBD	denotes	were
T6905	33851-33858	VBN	denotes	stained
T6907	33859-33863	IN	denotes	with
T6908	33864-33873	JJ	denotes	anti-ATRX
T6909	33874-33882	NN	denotes	antibody
T6910	33882-33884	,	denotes	,
T6911	33884-33889	IN	denotes	along
T6913	33890-33894	IN	denotes	with
T6912	33895-33903	NNS	denotes	sections
T6914	33904-33908	IN	denotes	from
T6915	33909-33910	DT	denotes	a
T6917	33911-33915	JJ	denotes	wild
T6919	33915-33916	HYPH	denotes	-
T6918	33916-33920	NN	denotes	type
T6920	33921-33924	CD	denotes	7.5
T6921	33925-33928	NN	denotes	dpc
T6916	33929-33935	NN	denotes	embryo
T6922	33936-33939	IN	denotes	for
T6923	33940-33950	NN	denotes	comparison
T6924	33951-33952	-LRB-	denotes	(
T6925	33952-33958	NN	denotes	Figure
T6926	33959-33960	CD	denotes	8
T6927	33960-33961	-RRB-	denotes	)
T6928	33961-33962	.	denotes	.
T6929	33962-34106	sentence	denotes	As described above, Atrx expression was detected in every cell in the epiblast (embryo proper) region of wild-type 7.5 dpc embryos (Figure 8B).
T6930	33963-33965	IN	denotes	As
T6931	33966-33975	VBN	denotes	described
T6933	33976-33981	RB	denotes	above
T6934	33981-33983	,	denotes	,
T6935	33983-33987	NN	denotes	Atrx
T6936	33988-33998	NN	denotes	expression
T6937	33999-34002	VBD	denotes	was
T6932	34003-34011	VBN	denotes	detected
T6938	34012-34014	IN	denotes	in
T6939	34015-34020	DT	denotes	every
T6940	34021-34025	NN	denotes	cell
T6941	34026-34028	IN	denotes	in
T6942	34029-34032	DT	denotes	the
T6944	34033-34041	NN	denotes	epiblast
T6945	34042-34043	-LRB-	denotes	(
T6946	34043-34049	NN	denotes	embryo
T6947	34050-34056	JJ	denotes	proper
T6948	34056-34057	-RRB-	denotes	)
T6943	34058-34064	NN	denotes	region
T6949	34065-34067	IN	denotes	of
T6950	34068-34072	JJ	denotes	wild
T6952	34072-34073	HYPH	denotes	-
T6951	34073-34077	NN	denotes	type
T6954	34078-34081	CD	denotes	7.5
T6955	34082-34085	NN	denotes	dpc
T6953	34086-34093	NNS	denotes	embryos
T6956	34094-34095	-LRB-	denotes	(
T6958	34095-34101	NN	denotes	Figure
T6957	34102-34104	NN	denotes	8B
T6959	34104-34105	-RRB-	denotes	)
T6960	34105-34106	.	denotes	.
T6961	34106-34463	sentence	denotes	In contrast, the epiblast region of carrier female embryos was composed of a mosaic of small clusters of Atrx-positive cells (in which the Atrx null allele on Xm had been inactivated) and Atrx-negative cells (in which the Atrx WT allele on Xp had been inactivated), indicating that the Atrx gene was subject to normal random X-inactivation in the epiblast.
T6962	34107-34109	IN	denotes	In
T6964	34110-34118	NN	denotes	contrast
T6965	34118-34120	,	denotes	,
T6966	34120-34123	DT	denotes	the
T6968	34124-34132	NN	denotes	epiblast
T6967	34133-34139	NN	denotes	region
T6969	34140-34142	IN	denotes	of
T6970	34143-34150	NN	denotes	carrier
T6972	34151-34157	JJ	denotes	female
T6971	34158-34165	NNS	denotes	embryos
T6973	34166-34169	VBD	denotes	was
T6963	34170-34178	VBN	denotes	composed
T6974	34179-34181	IN	denotes	of
T6975	34182-34183	DT	denotes	a
T6976	34184-34190	NN	denotes	mosaic
T6977	34191-34193	IN	denotes	of
T6978	34194-34199	JJ	denotes	small
T6979	34200-34208	NNS	denotes	clusters
T6980	34209-34211	IN	denotes	of
T6981	34212-34216	NN	denotes	Atrx
T6983	34216-34217	HYPH	denotes	-
T6982	34217-34225	JJ	denotes	positive
T6984	34226-34231	NNS	denotes	cells
T6985	34232-34233	-LRB-	denotes	(
T6986	34233-34235	IN	denotes	in
T6988	34236-34241	WDT	denotes	which
T6989	34242-34245	DT	denotes	the
T6991	34246-34255	JJ	denotes	Atrx null
T6990	34256-34262	NN	denotes	allele
T6992	34263-34265	IN	denotes	on
T6993	34266-34268	NN	denotes	Xm
T6994	34269-34272	VBD	denotes	had
T6995	34273-34277	VBN	denotes	been
T6987	34278-34289	VBN	denotes	inactivated
T6996	34289-34290	-RRB-	denotes	)
T6997	34291-34294	CC	denotes	and
T6998	34295-34299	NN	denotes	Atrx
T7000	34299-34300	HYPH	denotes	-
T6999	34300-34308	JJ	denotes	negative
T7001	34309-34314	NNS	denotes	cells
T7002	34315-34316	-LRB-	denotes	(
T7003	34316-34318	IN	denotes	in
T7005	34319-34324	WDT	denotes	which
T7006	34325-34328	DT	denotes	the
T7008	34329-34336	NN	denotes	Atrx WT
T7007	34337-34343	NN	denotes	allele
T7009	34344-34346	IN	denotes	on
T7010	34347-34349	NN	denotes	Xp
T7011	34350-34353	VBD	denotes	had
T7012	34354-34358	VBN	denotes	been
T7004	34359-34370	VBN	denotes	inactivated
T7013	34370-34371	-RRB-	denotes	)
T7014	34371-34373	,	denotes	,
T7015	34373-34383	VBG	denotes	indicating
T7016	34384-34388	IN	denotes	that
T7018	34389-34392	DT	denotes	the
T7020	34393-34397	NN	denotes	Atrx
T7019	34398-34402	NN	denotes	gene
T7017	34403-34406	VBD	denotes	was
T7021	34407-34414	JJ	denotes	subject
T7022	34415-34417	IN	denotes	to
T7023	34418-34424	JJ	denotes	normal
T7025	34425-34431	JJ	denotes	random
T7026	34432-34433	NN	denotes	X
T7027	34433-34434	HYPH	denotes	-
T7024	34434-34446	NN	denotes	inactivation
T7028	34447-34449	IN	denotes	in
T7029	34450-34453	DT	denotes	the
T7030	34454-34462	NN	denotes	epiblast
T7031	34462-34463	.	denotes	.
T7032	34463-34641	sentence	denotes	Remarkably, clear Atrx expression could also be detected in the extraembryonic tissues of carrier females, as shown in the extraembryonic-derived chorionic ectoderm (Figure 8C).
T7033	34464-34474	RB	denotes	Remarkably
T7035	34474-34476	,	denotes	,
T7036	34476-34481	JJ	denotes	clear
T7038	34482-34486	NN	denotes	Atrx
T7037	34487-34497	NN	denotes	expression
T7039	34498-34503	MD	denotes	could
T7040	34504-34508	RB	denotes	also
T7041	34509-34511	VB	denotes	be
T7034	34512-34520	VBN	denotes	detected
T7042	34521-34523	IN	denotes	in
T7043	34524-34527	DT	denotes	the
T7045	34528-34542	JJ	denotes	extraembryonic
T7044	34543-34550	NNS	denotes	tissues
T7046	34551-34553	IN	denotes	of
T7047	34554-34561	NN	denotes	carrier
T7048	34562-34569	NNS	denotes	females
T7049	34569-34571	,	denotes	,
T7050	34571-34573	IN	denotes	as
T7051	34574-34579	VBN	denotes	shown
T7052	34580-34582	IN	denotes	in
T7053	34583-34586	DT	denotes	the
T7055	34587-34601	JJ	denotes	extraembryonic
T7057	34601-34602	HYPH	denotes	-
T7056	34602-34609	VBN	denotes	derived
T7058	34610-34619	JJ	denotes	chorionic
T7054	34620-34628	NN	denotes	ectoderm
T7059	34629-34630	-LRB-	denotes	(
T7061	34630-34636	NN	denotes	Figure
T7060	34637-34639	NN	denotes	8C
T7062	34639-34640	-RRB-	denotes	)
T7063	34640-34641	.	denotes	.
T7064	34641-34722	sentence	denotes	Atrx expression could be detected in almost all cells of the chorionic ectoderm.
T7065	34642-34646	NN	denotes	Atrx
T7066	34647-34657	NN	denotes	expression
T7068	34658-34663	MD	denotes	could
T7069	34664-34666	VB	denotes	be
T7067	34667-34675	VBN	denotes	detected
T7070	34676-34678	IN	denotes	in
T7071	34679-34685	RB	denotes	almost
T7072	34686-34689	DT	denotes	all
T7073	34690-34695	NNS	denotes	cells
T7074	34696-34698	IN	denotes	of
T7075	34699-34702	DT	denotes	the
T7077	34703-34712	JJ	denotes	chorionic
T7076	34713-34721	NN	denotes	ectoderm
T7078	34721-34722	.	denotes	.
T7079	34722-34835	sentence	denotes	Atrx expression was also clearly detected in other extraembryonic structures, including TGCs (unpublished data).
T7080	34723-34727	NN	denotes	Atrx
T7081	34728-34738	NN	denotes	expression
T7083	34739-34742	VBD	denotes	was
T7084	34743-34747	RB	denotes	also
T7085	34748-34755	RB	denotes	clearly
T7082	34756-34764	VBN	denotes	detected
T7086	34765-34767	IN	denotes	in
T7087	34768-34773	JJ	denotes	other
T7089	34774-34788	JJ	denotes	extraembryonic
T7088	34789-34799	NNS	denotes	structures
T7090	34799-34801	,	denotes	,
T7091	34801-34810	VBG	denotes	including
T7092	34811-34815	NNS	denotes	TGCs
T7093	34816-34817	-LRB-	denotes	(
T7095	34817-34828	JJ	denotes	unpublished
T7094	34829-34833	NNS	denotes	data
T7096	34833-34834	-RRB-	denotes	)
T7097	34834-34835	.	denotes	.
T7098	34835-34978	sentence	denotes	Escape from silencing of an Xp-inherited Atrx WT allele was also observed in the chorionic ectoderm of carrier females at 8.5 dpc (Figure S4).
T7099	34836-34842	NN	denotes	Escape
T7101	34843-34847	IN	denotes	from
T7102	34848-34857	NN	denotes	silencing
T7103	34858-34860	IN	denotes	of
T7104	34861-34863	DT	denotes	an
T7106	34864-34866	NN	denotes	Xp
T7108	34866-34867	HYPH	denotes	-
T7107	34867-34876	VBN	denotes	inherited
T7109	34877-34884	NN	denotes	Atrx WT
T7105	34885-34891	NN	denotes	allele
T7110	34892-34895	VBD	denotes	was
T7111	34896-34900	RB	denotes	also
T7100	34901-34909	VBN	denotes	observed
T7112	34910-34912	IN	denotes	in
T7113	34913-34916	DT	denotes	the
T7115	34917-34926	JJ	denotes	chorionic
T7114	34927-34935	NN	denotes	ectoderm
T7116	34936-34938	IN	denotes	of
T7117	34939-34946	NN	denotes	carrier
T7118	34947-34954	NNS	denotes	females
T7119	34955-34957	IN	denotes	at
T7120	34958-34961	CD	denotes	8.5
T7121	34962-34965	NN	denotes	dpc
T7122	34966-34967	-LRB-	denotes	(
T7124	34967-34973	NN	denotes	Figure
T7123	34974-34976	NN	denotes	S4
T7125	34976-34977	-RRB-	denotes	)
T7126	34977-34978	.	denotes	.
T7127	34978-35207	sentence	denotes	Thus, although random X-inactivation occurs normally within the epiblast, the Atrx WT allele (inherited on the Xp chromosome) escaped the normal imprinted X-inactivation in the extraembryonic compartment of some carrier females.
T7128	34979-34983	RB	denotes	Thus
T7130	34983-34985	,	denotes	,
T7131	34985-34993	IN	denotes	although
T7133	34994-35000	JJ	denotes	random
T7135	35001-35002	NN	denotes	X
T7136	35002-35003	HYPH	denotes	-
T7134	35003-35015	NN	denotes	inactivation
T7132	35016-35022	VBZ	denotes	occurs
T7137	35023-35031	RB	denotes	normally
T7138	35032-35038	IN	denotes	within
T7139	35039-35042	DT	denotes	the
T7140	35043-35051	NN	denotes	epiblast
T7141	35051-35053	,	denotes	,
T7142	35053-35056	DT	denotes	the
T7144	35057-35064	NN	denotes	Atrx WT
T7143	35065-35071	NN	denotes	allele
T7145	35072-35073	-LRB-	denotes	(
T7146	35073-35082	VBN	denotes	inherited
T7147	35083-35085	IN	denotes	on
T7148	35086-35089	DT	denotes	the
T7150	35090-35092	NN	denotes	Xp
T7149	35093-35103	NN	denotes	chromosome
T7151	35103-35104	-RRB-	denotes	)
T7129	35105-35112	VBD	denotes	escaped
T7152	35113-35116	DT	denotes	the
T7154	35117-35123	JJ	denotes	normal
T7155	35124-35133	VBN	denotes	imprinted
T7156	35134-35135	NN	denotes	X
T7157	35135-35136	HYPH	denotes	-
T7153	35136-35148	NN	denotes	inactivation
T7158	35149-35151	IN	denotes	in
T7159	35152-35155	DT	denotes	the
T7161	35156-35170	JJ	denotes	extraembryonic
T7160	35171-35182	NN	denotes	compartment
T7162	35183-35185	IN	denotes	of
T7163	35186-35190	DT	denotes	some
T7165	35191-35198	NN	denotes	carrier
T7164	35199-35206	NNS	denotes	females
T7166	35206-35207	.	denotes	.
T7194	35981-35983	PRP	denotes	We
T7195	35984-35996	VBD	denotes	investigated
T7196	35997-36000	DT	denotes	the
T7197	36001-36005	NN	denotes	role
T7198	36006-36008	IN	denotes	of
T7199	36009-36012	DT	denotes	the
T7201	36013-36017	NN	denotes	Atrx
T7200	36018-36025	NN	denotes	protein
T7202	36026-36028	IN	denotes	in
T7203	36029-36034	NN	denotes	mouse
T7204	36035-36046	NN	denotes	development
T7205	36046-36047	.	denotes	.
T7206	36047-36206	sentence	denotes	By using a conditional knockout approach, we ablated the full-length Atrx protein first in ES cells and embryoid bodies, and then in developing mouse embryos.
T7207	36048-36050	IN	denotes	By
T7209	36051-36056	VBG	denotes	using
T7210	36057-36058	DT	denotes	a
T7212	36059-36070	JJ	denotes	conditional
T7213	36071-36079	NN	denotes	knockout
T7211	36080-36088	NN	denotes	approach
T7214	36088-36090	,	denotes	,
T7215	36090-36092	PRP	denotes	we
T7208	36093-36100	VBD	denotes	ablated
T7216	36101-36104	DT	denotes	the
T7218	36105-36109	JJ	denotes	full
T7220	36109-36110	HYPH	denotes	-
T7219	36110-36116	NN	denotes	length
T7221	36117-36121	NN	denotes	Atrx
T7217	36122-36129	NN	denotes	protein
T7222	36130-36135	RB	denotes	first
T7223	36136-36138	IN	denotes	in
T7224	36139-36141	NN	denotes	ES
T7225	36142-36147	NNS	denotes	cells
T7226	36148-36151	CC	denotes	and
T7227	36152-36160	JJ	denotes	embryoid
T7228	36161-36167	NNS	denotes	bodies
T7229	36167-36169	,	denotes	,
T7230	36169-36172	CC	denotes	and
T7231	36173-36177	RB	denotes	then
T7232	36178-36180	IN	denotes	in
T7233	36181-36191	VBG	denotes	developing
T7235	36192-36197	NN	denotes	mouse
T7234	36198-36205	NNS	denotes	embryos
T7236	36205-36206	.	denotes	.
T7415	36208-36212	NN	denotes	Atrx
T7416	36213-36215	IN	denotes	in
T7417	36216-36218	NN	denotes	ES
T7418	36219-36224	NNS	denotes	Cells
T7419	36224-36361	sentence	denotes	Atrxnull ES cells could not be recovered by direct targeting and were eventually generated by adopting a conditional targeting approach.
T7420	36225-36233	JJ	denotes	Atrxnull
T7421	36234-36236	NN	denotes	ES
T7422	36237-36242	NNS	denotes	cells
T7424	36243-36248	MD	denotes	could
T7425	36249-36252	RB	denotes	not
T7426	36253-36255	VB	denotes	be
T7423	36256-36265	VBN	denotes	recovered
T7427	36266-36268	IN	denotes	by
T7428	36269-36275	JJ	denotes	direct
T7429	36276-36285	NN	denotes	targeting
T7430	36286-36289	CC	denotes	and
T7431	36290-36294	VBD	denotes	were
T7433	36295-36305	RB	denotes	eventually
T7432	36306-36315	VBN	denotes	generated
T7434	36316-36318	IN	denotes	by
T7435	36319-36327	VBG	denotes	adopting
T7436	36328-36329	DT	denotes	a
T7438	36330-36341	JJ	denotes	conditional
T7439	36342-36351	NN	denotes	targeting
T7437	36352-36360	NN	denotes	approach
T7440	36360-36361	.	denotes	.
T7441	36361-36567	sentence	denotes	This is consistent with our observation that Atrx is highly expressed in ES cells, and that the absence of full-length Atrx imparts a growth disadvantage relative to cells bearing a functional Atrx allele.
T7442	36362-36366	DT	denotes	This
T7443	36367-36369	VBZ	denotes	is
T7444	36370-36380	JJ	denotes	consistent
T7445	36381-36385	IN	denotes	with
T7446	36386-36389	PRP$	denotes	our
T7447	36390-36401	NN	denotes	observation
T7448	36402-36406	IN	denotes	that
T7450	36407-36411	NN	denotes	Atrx
T7449	36412-36414	VBZ	denotes	is
T7451	36415-36421	RB	denotes	highly
T7452	36422-36431	JJ	denotes	expressed
T7453	36432-36434	IN	denotes	in
T7454	36435-36437	NN	denotes	ES
T7455	36438-36443	NNS	denotes	cells
T7456	36443-36445	,	denotes	,
T7457	36445-36448	CC	denotes	and
T7458	36449-36453	IN	denotes	that
T7460	36454-36457	DT	denotes	the
T7461	36458-36465	NN	denotes	absence
T7462	36466-36468	IN	denotes	of
T7463	36469-36473	JJ	denotes	full
T7465	36473-36474	HYPH	denotes	-
T7464	36474-36480	NN	denotes	length
T7466	36481-36485	NN	denotes	Atrx
T7459	36486-36493	VBZ	denotes	imparts
T7467	36494-36495	DT	denotes	a
T7469	36496-36502	NN	denotes	growth
T7468	36503-36515	NN	denotes	disadvantage
T7470	36516-36524	JJ	denotes	relative
T7471	36525-36527	IN	denotes	to
T7472	36528-36533	NNS	denotes	cells
T7473	36534-36541	VBG	denotes	bearing
T7474	36542-36543	DT	denotes	a
T7476	36544-36554	JJ	denotes	functional
T7477	36555-36559	NN	denotes	Atrx
T7475	36560-36566	NN	denotes	allele
T7478	36566-36567	.	denotes	.
T7479	36567-36651	sentence	denotes	At present, the cause of the proliferative delay in Atrxnull ES cells is not known.
T7480	36568-36570	IN	denotes	At
T7482	36571-36578	JJ	denotes	present
T7483	36578-36580	,	denotes	,
T7484	36580-36583	DT	denotes	the
T7485	36584-36589	NN	denotes	cause
T7486	36590-36592	IN	denotes	of
T7487	36593-36596	DT	denotes	the
T7489	36597-36610	JJ	denotes	proliferative
T7488	36611-36616	NN	denotes	delay
T7490	36617-36619	IN	denotes	in
T7491	36620-36628	JJ	denotes	Atrxnull
T7493	36629-36631	NN	denotes	ES
T7492	36632-36637	NNS	denotes	cells
T7481	36638-36640	VBZ	denotes	is
T7494	36641-36644	RB	denotes	not
T7495	36645-36650	JJ	denotes	known
T7496	36650-36651	.	denotes	.
T7497	36651-36819	sentence	denotes	Interestingly, we demonstrated that apoptosis is not significantly up-regulated in ES cells lacking Atrx and is only mildly elevated in Atrxnull 7.5 dpc mouse embryos.
T7498	36652-36665	RB	denotes	Interestingly
T7500	36665-36667	,	denotes	,
T7501	36667-36669	PRP	denotes	we
T7499	36670-36682	VBD	denotes	demonstrated
T7502	36683-36687	IN	denotes	that
T7504	36688-36697	NN	denotes	apoptosis
T7505	36698-36700	VBZ	denotes	is
T7506	36701-36704	RB	denotes	not
T7507	36705-36718	RB	denotes	significantly
T7508	36719-36721	RB	denotes	up
T7509	36721-36722	HYPH	denotes	-
T7503	36722-36731	VBN	denotes	regulated
T7510	36732-36734	IN	denotes	in
T7511	36735-36737	NN	denotes	ES
T7512	36738-36743	NNS	denotes	cells
T7513	36744-36751	VBG	denotes	lacking
T7514	36752-36756	NN	denotes	Atrx
T7515	36757-36760	CC	denotes	and
T7516	36761-36763	VBZ	denotes	is
T7517	36764-36768	RB	denotes	only
T7518	36769-36775	RB	denotes	mildly
T7519	36776-36784	JJ	denotes	elevated
T7520	36785-36787	IN	denotes	in
T7521	36788-36796	JJ	denotes	Atrxnull
T7523	36797-36800	CD	denotes	7.5
T7524	36801-36804	NN	denotes	dpc
T7525	36805-36810	NN	denotes	mouse
T7522	36811-36818	NNS	denotes	embryos
T7526	36818-36819	.	denotes	.
T7527	36819-37115	sentence	denotes	In contrast, it was recently shown that the loss of Atrx markedly increased the apoptotic population in the differentiating cells of the embryonic cortex and postnatal hippocampus, when Atrx expression was ablated in the developing mouse forebrain using the Atrx flox allele described here [20].
T7528	36820-36822	IN	denotes	In
T7530	36823-36831	NN	denotes	contrast
T7531	36831-36833	,	denotes	,
T7532	36833-36835	PRP	denotes	it
T7533	36836-36839	VBD	denotes	was
T7534	36840-36848	RB	denotes	recently
T7529	36849-36854	VBN	denotes	shown
T7535	36855-36859	IN	denotes	that
T7537	36860-36863	DT	denotes	the
T7538	36864-36868	NN	denotes	loss
T7539	36869-36871	IN	denotes	of
T7540	36872-36876	NN	denotes	Atrx
T7541	36877-36885	RB	denotes	markedly
T7536	36886-36895	VBD	denotes	increased
T7542	36896-36899	DT	denotes	the
T7544	36900-36909	JJ	denotes	apoptotic
T7543	36910-36920	NN	denotes	population
T7545	36921-36923	IN	denotes	in
T7546	36924-36927	DT	denotes	the
T7548	36928-36943	VBG	denotes	differentiating
T7547	36944-36949	NNS	denotes	cells
T7549	36950-36952	IN	denotes	of
T7550	36953-36956	DT	denotes	the
T7552	36957-36966	JJ	denotes	embryonic
T7551	36967-36973	NN	denotes	cortex
T7553	36974-36977	CC	denotes	and
T7554	36978-36987	JJ	denotes	postnatal
T7555	36988-36999	NN	denotes	hippocampus
T7556	36999-37001	,	denotes	,
T7557	37001-37005	WRB	denotes	when
T7559	37006-37010	NN	denotes	Atrx
T7560	37011-37021	NN	denotes	expression
T7561	37022-37025	VBD	denotes	was
T7558	37026-37033	VBN	denotes	ablated
T7562	37034-37036	IN	denotes	in
T7563	37037-37040	DT	denotes	the
T7565	37041-37051	VBG	denotes	developing
T7566	37052-37057	NN	denotes	mouse
T7564	37058-37067	NN	denotes	forebrain
T7567	37068-37073	VBG	denotes	using
T7568	37074-37077	DT	denotes	the
T7570	37078-37087	NN	denotes	Atrx flox
T7569	37088-37094	NN	denotes	allele
T7571	37095-37104	VBN	denotes	described
T7572	37105-37109	RB	denotes	here
T7573	37110-37111	-LRB-	denotes	[
T7574	37111-37113	CD	denotes	20
T7575	37113-37114	-RRB-	denotes	]
T7576	37114-37115	.	denotes	.
T7577	37115-37288	sentence	denotes	The human ATRX protein has been shown to associate in a complex with Daxx [8], a protein that has been implicated in multiple pathways for the regulation of apoptosis [21].
T7578	37116-37119	DT	denotes	The
T7580	37120-37125	JJ	denotes	human
T7581	37126-37130	NN	denotes	ATRX
T7579	37131-37138	NN	denotes	protein
T7583	37139-37142	VBZ	denotes	has
T7584	37143-37147	VBN	denotes	been
T7582	37148-37153	VBN	denotes	shown
T7585	37154-37156	TO	denotes	to
T7586	37157-37166	VB	denotes	associate
T7587	37167-37169	IN	denotes	in
T7588	37170-37171	DT	denotes	a
T7589	37172-37179	NN	denotes	complex
T7590	37180-37184	IN	denotes	with
T7591	37185-37189	NN	denotes	Daxx
T7592	37190-37191	-LRB-	denotes	[
T7593	37191-37192	CD	denotes	8
T7594	37192-37193	-RRB-	denotes	]
T7595	37193-37195	,	denotes	,
T7596	37195-37196	DT	denotes	a
T7597	37197-37204	NN	denotes	protein
T7598	37205-37209	WDT	denotes	that
T7600	37210-37213	VBZ	denotes	has
T7601	37214-37218	VBN	denotes	been
T7599	37219-37229	VBN	denotes	implicated
T7602	37230-37232	IN	denotes	in
T7603	37233-37241	JJ	denotes	multiple
T7604	37242-37250	NNS	denotes	pathways
T7605	37251-37254	IN	denotes	for
T7606	37255-37258	DT	denotes	the
T7607	37259-37269	NN	denotes	regulation
T7608	37270-37272	IN	denotes	of
T7609	37273-37282	NN	denotes	apoptosis
T7610	37283-37284	-LRB-	denotes	[
T7611	37284-37286	CD	denotes	21
T7612	37286-37287	-RRB-	denotes	]
T7613	37287-37288	.	denotes	.
T7614	37288-37440	sentence	denotes	It is possible that disruption of the mouse Atrx-Daxx complex (by ablation of the Atrx protein) could have triggered a universal proapoptotic response.
T7615	37289-37291	PRP	denotes	It
T7616	37292-37294	VBZ	denotes	is
T7617	37295-37303	JJ	denotes	possible
T7618	37304-37308	IN	denotes	that
T7620	37309-37319	NN	denotes	disruption
T7621	37320-37322	IN	denotes	of
T7622	37323-37326	DT	denotes	the
T7624	37327-37332	NN	denotes	mouse
T7625	37333-37337	NN	denotes	Atrx
T7627	37337-37338	HYPH	denotes	-
T7626	37338-37342	NN	denotes	Daxx
T7623	37343-37350	NN	denotes	complex
T7628	37351-37352	-LRB-	denotes	(
T7629	37352-37354	IN	denotes	by
T7630	37355-37363	NN	denotes	ablation
T7631	37364-37366	IN	denotes	of
T7632	37367-37370	DT	denotes	the
T7634	37371-37375	NN	denotes	Atrx
T7633	37376-37383	NN	denotes	protein
T7635	37383-37384	-RRB-	denotes	)
T7636	37385-37390	MD	denotes	could
T7637	37391-37395	VB	denotes	have
T7619	37396-37405	VBN	denotes	triggered
T7638	37406-37407	DT	denotes	a
T7640	37408-37417	JJ	denotes	universal
T7641	37418-37430	JJ	denotes	proapoptotic
T7639	37431-37439	NN	denotes	response
T7642	37439-37440	.	denotes	.
T7643	37440-37754	sentence	denotes	However, our observations in ES cells demonstrate that the induction of apoptosis is not an automatic response triggered by the removal of Atrx in all cell types, and suggest that the inappropriate apoptosis observed in the Atrx-mutant forebrain may reflect a requirement for Atrx during terminal differentiation.
T7644	37441-37448	RB	denotes	However
T7646	37448-37450	,	denotes	,
T7647	37450-37453	PRP$	denotes	our
T7648	37454-37466	NNS	denotes	observations
T7649	37467-37469	IN	denotes	in
T7650	37470-37472	NN	denotes	ES
T7651	37473-37478	NNS	denotes	cells
T7645	37479-37490	VBP	denotes	demonstrate
T7652	37491-37495	IN	denotes	that
T7654	37496-37499	DT	denotes	the
T7655	37500-37509	NN	denotes	induction
T7656	37510-37512	IN	denotes	of
T7657	37513-37522	NN	denotes	apoptosis
T7653	37523-37525	VBZ	denotes	is
T7658	37526-37529	RB	denotes	not
T7659	37530-37532	DT	denotes	an
T7661	37533-37542	JJ	denotes	automatic
T7660	37543-37551	NN	denotes	response
T7662	37552-37561	VBN	denotes	triggered
T7663	37562-37564	IN	denotes	by
T7664	37565-37568	DT	denotes	the
T7665	37569-37576	NN	denotes	removal
T7666	37577-37579	IN	denotes	of
T7667	37580-37584	NN	denotes	Atrx
T7668	37585-37587	IN	denotes	in
T7669	37588-37591	DT	denotes	all
T7671	37592-37596	NN	denotes	cell
T7670	37597-37602	NNS	denotes	types
T7672	37602-37604	,	denotes	,
T7673	37604-37607	CC	denotes	and
T7674	37608-37615	VBP	denotes	suggest
T7675	37616-37620	IN	denotes	that
T7677	37621-37624	DT	denotes	the
T7679	37625-37638	JJ	denotes	inappropriate
T7678	37639-37648	NN	denotes	apoptosis
T7680	37649-37657	VBN	denotes	observed
T7681	37658-37660	IN	denotes	in
T7682	37661-37664	DT	denotes	the
T7684	37665-37669	NN	denotes	Atrx
T7686	37669-37670	HYPH	denotes	-
T7685	37670-37676	NN	denotes	mutant
T7683	37677-37686	NN	denotes	forebrain
T7687	37687-37690	MD	denotes	may
T7676	37691-37698	VB	denotes	reflect
T7688	37699-37700	DT	denotes	a
T7689	37701-37712	NN	denotes	requirement
T7690	37713-37716	IN	denotes	for
T7691	37717-37721	NN	denotes	Atrx
T7692	37722-37728	IN	denotes	during
T7693	37729-37737	JJ	denotes	terminal
T7694	37738-37753	NN	denotes	differentiation
T7695	37753-37754	.	denotes	.
T8323	37756-37758	DT	denotes	An
T8325	37759-37769	JJ	denotes	Unexpected
T8324	37770-37774	NN	denotes	Role
T8326	37775-37778	IN	denotes	for
T8327	37779-37783	NN	denotes	Atrx
T8328	37784-37786	IN	denotes	in
T8329	37787-37798	NN	denotes	Development
T8330	37799-37801	IN	denotes	of
T8331	37802-37805	DT	denotes	the
T8333	37806-37811	NN	denotes	Mouse
T8332	37812-37823	NN	denotes	Trophoblast
T8334	37823-37905	sentence	denotes	We show that Atrxnull male mice are not viable and embryos die by around 9.5 dpc.
T8335	37824-37826	PRP	denotes	We
T8336	37827-37831	VBP	denotes	show
T8337	37832-37836	IN	denotes	that
T8339	37837-37845	JJ	denotes	Atrxnull
T8341	37846-37850	JJ	denotes	male
T8340	37851-37855	NNS	denotes	mice
T8338	37856-37859	VBP	denotes	are
T8342	37860-37863	RB	denotes	not
T8343	37864-37870	JJ	denotes	viable
T8344	37871-37874	CC	denotes	and
T8345	37875-37882	NNS	denotes	embryos
T8346	37883-37886	VBP	denotes	die
T8347	37887-37889	IN	denotes	by
T8348	37890-37896	IN	denotes	around
T8349	37897-37900	CD	denotes	9.5
T8350	37901-37904	NN	denotes	dpc
T8351	37904-37905	.	denotes	.
T8352	37905-38013	sentence	denotes	Before death, Atrxnull embryos exhibit a markedly reduced mitotic index, suggesting a proliferative defect.
T8353	37906-37912	IN	denotes	Before
T8355	37913-37918	NN	denotes	death
T8356	37918-37920	,	denotes	,
T8357	37920-37928	JJ	denotes	Atrxnull
T8358	37929-37936	NNS	denotes	embryos
T8354	37937-37944	VBP	denotes	exhibit
T8359	37945-37946	DT	denotes	a
T8361	37947-37955	RB	denotes	markedly
T8362	37956-37963	VBN	denotes	reduced
T8363	37964-37971	JJ	denotes	mitotic
T8360	37972-37977	NN	denotes	index
T8364	37977-37979	,	denotes	,
T8365	37979-37989	VBG	denotes	suggesting
T8366	37990-37991	DT	denotes	a
T8368	37992-38005	JJ	denotes	proliferative
T8367	38006-38012	NN	denotes	defect
T8369	38012-38013	.	denotes	.
T8370	38013-38360	sentence	denotes	Although the embryonic compartment of the conceptus appears initially normal, by 7.5 dpc Atrxnull embryos display abnormalities in development of the trophoblast, including a depletion in the population of TGCs surrounding the conceptus and a reduction in the size of the ectoplacental cone, which contains the diploid giant cell precursors [22].
T8371	38014-38022	IN	denotes	Although
T8373	38023-38026	DT	denotes	the
T8375	38027-38036	JJ	denotes	embryonic
T8374	38037-38048	NN	denotes	compartment
T8376	38049-38051	IN	denotes	of
T8377	38052-38055	DT	denotes	the
T8378	38056-38065	NN	denotes	conceptus
T8372	38066-38073	VBZ	denotes	appears
T8380	38074-38083	RB	denotes	initially
T8381	38084-38090	JJ	denotes	normal
T8382	38090-38092	,	denotes	,
T8383	38092-38094	IN	denotes	by
T8384	38095-38098	CD	denotes	7.5
T8385	38099-38102	NN	denotes	dpc
T8386	38103-38111	JJ	denotes	Atrxnull
T8387	38112-38119	NNS	denotes	embryos
T8379	38120-38127	VBP	denotes	display
T8388	38128-38141	NNS	denotes	abnormalities
T8389	38142-38144	IN	denotes	in
T8390	38145-38156	NN	denotes	development
T8391	38157-38159	IN	denotes	of
T8392	38160-38163	DT	denotes	the
T8393	38164-38175	NN	denotes	trophoblast
T8394	38175-38177	,	denotes	,
T8395	38177-38186	VBG	denotes	including
T8396	38187-38188	DT	denotes	a
T8397	38189-38198	NN	denotes	depletion
T8398	38199-38201	IN	denotes	in
T8399	38202-38205	DT	denotes	the
T8400	38206-38216	NN	denotes	population
T8401	38217-38219	IN	denotes	of
T8402	38220-38224	NNS	denotes	TGCs
T8403	38225-38236	VBG	denotes	surrounding
T8404	38237-38240	DT	denotes	the
T8405	38241-38250	NN	denotes	conceptus
T8406	38251-38254	CC	denotes	and
T8407	38255-38256	DT	denotes	a
T8408	38257-38266	NN	denotes	reduction
T8409	38267-38269	IN	denotes	in
T8410	38270-38273	DT	denotes	the
T8411	38274-38278	NN	denotes	size
T8412	38279-38281	IN	denotes	of
T8413	38282-38285	DT	denotes	the
T8415	38286-38299	JJ	denotes	ectoplacental
T8414	38300-38304	NN	denotes	cone
T8416	38304-38306	,	denotes	,
T8417	38306-38311	WDT	denotes	which
T8418	38312-38320	VBZ	denotes	contains
T8419	38321-38324	DT	denotes	the
T8421	38325-38332	NN	denotes	diploid
T8422	38333-38338	JJ	denotes	giant
T8423	38339-38343	NN	denotes	cell
T8420	38344-38354	NNS	denotes	precursors
T8424	38355-38356	-LRB-	denotes	[
T8425	38356-38358	CD	denotes	22
T8426	38358-38359	-RRB-	denotes	]
T8427	38359-38360	.	denotes	.
T8428	38360-38547	sentence	denotes	TGCs are highly differentiated, postmitotic cells that ultimately form an epithelial layer at the periphery of the placenta, which interfaces with the maternal cells of the decidua [23].
T8429	38361-38365	NNS	denotes	TGCs
T8430	38366-38369	VBP	denotes	are
T8431	38370-38376	RB	denotes	highly
T8432	38377-38391	VBN	denotes	differentiated
T8434	38391-38393	,	denotes	,
T8435	38393-38404	JJ	denotes	postmitotic
T8433	38405-38410	NNS	denotes	cells
T8436	38411-38415	WDT	denotes	that
T8438	38416-38426	RB	denotes	ultimately
T8437	38427-38431	VBP	denotes	form
T8439	38432-38434	DT	denotes	an
T8441	38435-38445	JJ	denotes	epithelial
T8440	38446-38451	NN	denotes	layer
T8442	38452-38454	IN	denotes	at
T8443	38455-38458	DT	denotes	the
T8444	38459-38468	NN	denotes	periphery
T8445	38469-38471	IN	denotes	of
T8446	38472-38475	DT	denotes	the
T8447	38476-38484	NN	denotes	placenta
T8448	38484-38486	,	denotes	,
T8449	38486-38491	WDT	denotes	which
T8450	38492-38502	VBZ	denotes	interfaces
T8451	38503-38507	IN	denotes	with
T8452	38508-38511	DT	denotes	the
T8454	38512-38520	JJ	denotes	maternal
T8453	38521-38526	NNS	denotes	cells
T8455	38527-38529	IN	denotes	of
T8456	38530-38533	DT	denotes	the
T8457	38534-38541	NN	denotes	decidua
T8458	38542-38543	-LRB-	denotes	[
T8459	38543-38545	CD	denotes	23
T8460	38545-38546	-RRB-	denotes	]
T8461	38546-38547	.	denotes	.
T8462	38547-38792	sentence	denotes	These highly invasive cells are important for mediating initial invasion of the uterine tissue, but are also involved in remodelling the maternal decidua after implantation and in secreting hormones that regulate fetal and maternal growth [24].
T8463	38548-38553	DT	denotes	These
T8465	38554-38560	RB	denotes	highly
T8466	38561-38569	JJ	denotes	invasive
T8464	38570-38575	NNS	denotes	cells
T8467	38576-38579	VBP	denotes	are
T8468	38580-38589	JJ	denotes	important
T8469	38590-38593	IN	denotes	for
T8470	38594-38603	VBG	denotes	mediating
T8471	38604-38611	JJ	denotes	initial
T8472	38612-38620	NN	denotes	invasion
T8473	38621-38623	IN	denotes	of
T8474	38624-38627	DT	denotes	the
T8476	38628-38635	JJ	denotes	uterine
T8475	38636-38642	NN	denotes	tissue
T8477	38642-38644	,	denotes	,
T8478	38644-38647	CC	denotes	but
T8479	38648-38651	VBP	denotes	are
T8481	38652-38656	RB	denotes	also
T8480	38657-38665	VBN	denotes	involved
T8482	38666-38668	IN	denotes	in
T8483	38669-38680	VBG	denotes	remodelling
T8484	38681-38684	DT	denotes	the
T8486	38685-38693	JJ	denotes	maternal
T8485	38694-38701	NN	denotes	decidua
T8487	38702-38707	IN	denotes	after
T8488	38708-38720	NN	denotes	implantation
T8489	38721-38724	CC	denotes	and
T8490	38725-38727	IN	denotes	in
T8491	38728-38737	VBG	denotes	secreting
T8492	38738-38746	NNS	denotes	hormones
T8493	38747-38751	WDT	denotes	that
T8494	38752-38760	VBP	denotes	regulate
T8495	38761-38766	JJ	denotes	fetal
T8497	38767-38770	CC	denotes	and
T8498	38771-38779	JJ	denotes	maternal
T8496	38780-38786	NN	denotes	growth
T8499	38787-38788	-LRB-	denotes	[
T8500	38788-38790	CD	denotes	24
T8501	38790-38791	-RRB-	denotes	]
T8502	38791-38792	.	denotes	.
T8503	38792-38931	sentence	denotes	Since Atrxnull embryos appear to implant normally, lethality is likely to arise due to a failure of TGC function later during development.
T8504	38793-38798	IN	denotes	Since
T8506	38799-38807	JJ	denotes	Atrxnull
T8507	38808-38815	NNS	denotes	embryos
T8505	38816-38822	VBP	denotes	appear
T8509	38823-38825	TO	denotes	to
T8510	38826-38833	VB	denotes	implant
T8511	38834-38842	RB	denotes	normally
T8512	38842-38844	,	denotes	,
T8513	38844-38853	NN	denotes	lethality
T8508	38854-38856	VBZ	denotes	is
T8514	38857-38863	JJ	denotes	likely
T8515	38864-38866	TO	denotes	to
T8516	38867-38872	VB	denotes	arise
T8517	38873-38876	IN	denotes	due
T8518	38877-38879	IN	denotes	to
T8519	38880-38881	DT	denotes	a
T8520	38882-38889	NN	denotes	failure
T8521	38890-38892	IN	denotes	of
T8522	38893-38896	NN	denotes	TGC
T8523	38897-38905	NN	denotes	function
T8524	38906-38911	RB	denotes	later
T8525	38912-38918	IN	denotes	during
T8526	38919-38930	NN	denotes	development
T8527	38930-38931	.	denotes	.
T8528	38931-39084	sentence	denotes	Embryonic lethality in mice in the absence of Atrx was a surprising finding, as there had been no suggestion of foetal loss in the human ATR-X syndrome.
T8529	38932-38941	JJ	denotes	Embryonic
T8530	38942-38951	NN	denotes	lethality
T8532	38952-38954	IN	denotes	in
T8533	38955-38959	NNS	denotes	mice
T8534	38960-38962	IN	denotes	in
T8535	38963-38966	DT	denotes	the
T8536	38967-38974	NN	denotes	absence
T8537	38975-38977	IN	denotes	of
T8538	38978-38982	NN	denotes	Atrx
T8531	38983-38986	VBD	denotes	was
T8539	38987-38988	DT	denotes	a
T8541	38989-38999	JJ	denotes	surprising
T8540	39000-39007	NN	denotes	finding
T8542	39007-39009	,	denotes	,
T8543	39009-39011	IN	denotes	as
T8545	39012-39017	EX	denotes	there
T8546	39018-39021	VBD	denotes	had
T8544	39022-39026	VBN	denotes	been
T8547	39027-39029	DT	denotes	no
T8548	39030-39040	NN	denotes	suggestion
T8549	39041-39043	IN	denotes	of
T8550	39044-39050	JJ	denotes	foetal
T8551	39051-39055	NN	denotes	loss
T8552	39056-39058	IN	denotes	in
T8553	39059-39062	DT	denotes	the
T8555	39063-39068	JJ	denotes	human
T8556	39069-39072	NN	denotes	ATR
T8558	39072-39073	HYPH	denotes	-
T8557	39073-39074	NN	denotes	X
T8554	39075-39083	NN	denotes	syndrome
T8559	39083-39084	.	denotes	.
T8560	39084-39228	sentence	denotes	It is possible that the role of Atrx in the trophoblast is specific to mice and that ATRX has no role or is redundant in the human trophoblast.
T8561	39085-39087	PRP	denotes	It
T8562	39088-39090	VBZ	denotes	is
T8563	39091-39099	JJ	denotes	possible
T8564	39100-39104	IN	denotes	that
T8566	39105-39108	DT	denotes	the
T8567	39109-39113	NN	denotes	role
T8568	39114-39116	IN	denotes	of
T8569	39117-39121	NN	denotes	Atrx
T8570	39122-39124	IN	denotes	in
T8571	39125-39128	DT	denotes	the
T8572	39129-39140	NN	denotes	trophoblast
T8565	39141-39143	VBZ	denotes	is
T8573	39144-39152	JJ	denotes	specific
T8574	39153-39155	IN	denotes	to
T8575	39156-39160	NNS	denotes	mice
T8576	39161-39164	CC	denotes	and
T8577	39165-39169	IN	denotes	that
T8579	39170-39174	NN	denotes	ATRX
T8578	39175-39178	VBZ	denotes	has
T8580	39179-39181	DT	denotes	no
T8581	39182-39186	NN	denotes	role
T8582	39187-39189	CC	denotes	or
T8583	39190-39192	VBZ	denotes	is
T8584	39193-39202	JJ	denotes	redundant
T8585	39203-39205	IN	denotes	in
T8586	39206-39209	DT	denotes	the
T8588	39210-39215	JJ	denotes	human
T8587	39216-39227	NN	denotes	trophoblast
T8589	39227-39228	.	denotes	.
T8590	39228-39302	sentence	denotes	Indeed, the birth weight of babies with ATR-X syndrome is usually normal.
T8591	39229-39235	RB	denotes	Indeed
T8593	39235-39237	,	denotes	,
T8594	39237-39240	DT	denotes	the
T8596	39241-39246	NN	denotes	birth
T8595	39247-39253	NN	denotes	weight
T8597	39254-39256	IN	denotes	of
T8598	39257-39263	NNS	denotes	babies
T8599	39264-39268	IN	denotes	with
T8600	39269-39272	NN	denotes	ATR
T8602	39272-39273	HYPH	denotes	-
T8601	39273-39274	NN	denotes	X
T8603	39275-39283	NN	denotes	syndrome
T8592	39284-39286	VBZ	denotes	is
T8604	39287-39294	RB	denotes	usually
T8605	39295-39301	JJ	denotes	normal
T8606	39301-39302	.	denotes	.
T8607	39302-39509	sentence	denotes	An alternative explanation for the unexpectedly severe phenotype we observed in mice is that the Atrx Δ18Δneo deletion generated by Cre recombination completely ablates full-length Atrx protein (Figure 2E).
T8608	39303-39305	DT	denotes	An
T8610	39306-39317	JJ	denotes	alternative
T8609	39318-39329	NN	denotes	explanation
T8612	39330-39333	IN	denotes	for
T8613	39334-39337	DT	denotes	the
T8615	39338-39350	RB	denotes	unexpectedly
T8616	39351-39357	JJ	denotes	severe
T8614	39358-39367	NN	denotes	phenotype
T8617	39368-39370	PRP	denotes	we
T8618	39371-39379	VBD	denotes	observed
T8619	39380-39382	IN	denotes	in
T8620	39383-39387	NNS	denotes	mice
T8611	39388-39390	VBZ	denotes	is
T8621	39391-39395	IN	denotes	that
T8623	39396-39399	DT	denotes	the
T8625	39400-39412	NN	denotes	Atrx Δ18Δneo
T8624	39413-39421	NN	denotes	deletion
T8626	39422-39431	VBN	denotes	generated
T8627	39432-39434	IN	denotes	by
T8628	39435-39438	NN	denotes	Cre
T8629	39439-39452	NN	denotes	recombination
T8630	39453-39463	RB	denotes	completely
T8622	39464-39471	VBZ	denotes	ablates
T8631	39472-39476	JJ	denotes	full
T8633	39476-39477	HYPH	denotes	-
T8632	39477-39483	NN	denotes	length
T8635	39484-39488	NN	denotes	Atrx
T8634	39489-39496	NN	denotes	protein
T8636	39497-39498	-LRB-	denotes	(
T8638	39498-39504	NN	denotes	Figure
T8637	39505-39507	NN	denotes	2E
T8639	39507-39508	-RRB-	denotes	)
T8640	39508-39509	.	denotes	.
T8641	39509-39635	sentence	denotes	In contrast, all disease-causing mutations characterised in human ATR-X pedigrees appear to give rise to hypomorphic alleles.
T8642	39510-39512	IN	denotes	In
T8644	39513-39521	NN	denotes	contrast
T8645	39521-39523	,	denotes	,
T8646	39523-39526	DT	denotes	all
T8648	39527-39534	NN	denotes	disease
T8650	39534-39535	HYPH	denotes	-
T8649	39535-39542	VBG	denotes	causing
T8647	39543-39552	NNS	denotes	mutations
T8651	39553-39566	VBN	denotes	characterised
T8652	39567-39569	IN	denotes	in
T8653	39570-39575	JJ	denotes	human
T8655	39576-39579	NN	denotes	ATR
T8657	39579-39580	HYPH	denotes	-
T8656	39580-39581	NN	denotes	X
T8654	39582-39591	NNS	denotes	pedigrees
T8643	39592-39598	VBP	denotes	appear
T8658	39599-39601	TO	denotes	to
T8659	39602-39606	VB	denotes	give
T8660	39607-39611	NN	denotes	rise
T8661	39612-39614	IN	denotes	to
T8662	39615-39626	JJ	denotes	hypomorphic
T8663	39627-39634	NNS	denotes	alleles
T8664	39634-39635	.	denotes	.
T8665	39635-39955	sentence	denotes	Some full-length ATRX protein is detected in cases predicted to have truncating mutations (RJG, unpublished data), and residual ATPase activity in ATRX immunoprecipitates can be detected in Epstein-Barr virus-transformed lymphocytes of all human patients analysed to date (A. Argentaro and M. Mitson, unpublished data).
T8666	39636-39640	DT	denotes	Some
T8668	39641-39645	JJ	denotes	full
T8670	39645-39646	HYPH	denotes	-
T8669	39646-39652	NN	denotes	length
T8671	39653-39657	NN	denotes	ATRX
T8667	39658-39665	NN	denotes	protein
T8673	39666-39668	VBZ	denotes	is
T8672	39669-39677	VBN	denotes	detected
T8674	39678-39680	IN	denotes	in
T8675	39681-39686	NNS	denotes	cases
T8676	39687-39696	VBN	denotes	predicted
T8677	39697-39699	TO	denotes	to
T8678	39700-39704	VB	denotes	have
T8679	39705-39715	NN	denotes	truncating
T8680	39716-39725	NNS	denotes	mutations
T8681	39726-39727	-LRB-	denotes	(
T8683	39727-39730	NN	denotes	RJG
T8684	39730-39732	,	denotes	,
T8685	39732-39743	JJ	denotes	unpublished
T8682	39744-39748	NNS	denotes	data
T8686	39748-39749	-RRB-	denotes	)
T8687	39749-39751	,	denotes	,
T8688	39751-39754	CC	denotes	and
T8689	39755-39763	JJ	denotes	residual
T8691	39764-39770	NN	denotes	ATPase
T8690	39771-39779	NN	denotes	activity
T8693	39780-39782	IN	denotes	in
T8694	39783-39787	NN	denotes	ATRX
T8695	39788-39806	NNS	denotes	immunoprecipitates
T8696	39807-39810	MD	denotes	can
T8697	39811-39813	VB	denotes	be
T8692	39814-39822	VBN	denotes	detected
T8698	39823-39825	IN	denotes	in
T8699	39826-39833	NN	denotes	Epstein
T8701	39833-39834	HYPH	denotes	-
T8700	39834-39838	NN	denotes	Barr
T8702	39839-39844	NN	denotes	virus
T8704	39844-39845	HYPH	denotes	-
T8703	39845-39856	VBN	denotes	transformed
T8705	39857-39868	NNS	denotes	lymphocytes
T8706	39869-39871	IN	denotes	of
T8707	39872-39875	DT	denotes	all
T8709	39876-39881	JJ	denotes	human
T8708	39882-39890	NNS	denotes	patients
T8710	39891-39899	VBN	denotes	analysed
T8711	39900-39902	IN	denotes	to
T8712	39903-39907	NN	denotes	date
T8713	39908-39909	-LRB-	denotes	(
T8714	39909-39911	NNP	denotes	A.
T8715	39912-39921	NNP	denotes	Argentaro
T8716	39922-39925	CC	denotes	and
T8717	39926-39928	NNP	denotes	M.
T8718	39929-39935	NNP	denotes	Mitson
T8719	39935-39937	,	denotes	,
T8720	39937-39948	JJ	denotes	unpublished
T8721	39949-39953	NNS	denotes	data
T8722	39953-39954	-RRB-	denotes	)
T8723	39954-39955	.	denotes	.
T8724	39955-40145	sentence	denotes	The failure to observe a truly null ATRX allele among human patients strongly suggests that, as in the mouse, the complete absence of ATRX protein is incompatible with human fetal survival.
T8725	39956-39959	DT	denotes	The
T8726	39960-39967	NN	denotes	failure
T8728	39968-39970	TO	denotes	to
T8729	39971-39978	VB	denotes	observe
T8730	39979-39980	DT	denotes	a
T8732	39981-39986	RB	denotes	truly
T8733	39987-39991	JJ	denotes	null
T8734	39992-39996	NN	denotes	ATRX
T8731	39997-40003	NN	denotes	allele
T8735	40004-40009	IN	denotes	among
T8736	40010-40015	JJ	denotes	human
T8737	40016-40024	NNS	denotes	patients
T8738	40025-40033	RB	denotes	strongly
T8727	40034-40042	VBZ	denotes	suggests
T8739	40043-40047	IN	denotes	that
T8741	40047-40049	,	denotes	,
T8742	40049-40051	IN	denotes	as
T8743	40052-40054	IN	denotes	in
T8744	40055-40058	DT	denotes	the
T8745	40059-40064	NN	denotes	mouse
T8746	40064-40066	,	denotes	,
T8747	40066-40069	DT	denotes	the
T8749	40070-40078	JJ	denotes	complete
T8748	40079-40086	NN	denotes	absence
T8750	40087-40089	IN	denotes	of
T8751	40090-40094	NN	denotes	ATRX
T8752	40095-40102	NN	denotes	protein
T8740	40103-40105	VBZ	denotes	is
T8753	40106-40118	JJ	denotes	incompatible
T8754	40119-40123	IN	denotes	with
T8755	40124-40129	JJ	denotes	human
T8757	40130-40135	JJ	denotes	fetal
T8756	40136-40144	NN	denotes	survival
T8758	40144-40145	.	denotes	.
T8759	40145-40411	sentence	denotes	While this study has revealed an unexpected role for Atrx in the murine trophectoderm, as a result of the early lethality observed in Atrxnull embryos it is not possible to rule out other roles for Atrx at later developmental stages in tissues of the embryo proper.
T8760	40146-40151	IN	denotes	While
T8762	40152-40156	DT	denotes	this
T8763	40157-40162	NN	denotes	study
T8764	40163-40166	VBZ	denotes	has
T8761	40167-40175	VBN	denotes	revealed
T8766	40176-40178	DT	denotes	an
T8768	40179-40189	JJ	denotes	unexpected
T8767	40190-40194	NN	denotes	role
T8769	40195-40198	IN	denotes	for
T8770	40199-40203	NN	denotes	Atrx
T8771	40204-40206	IN	denotes	in
T8772	40207-40210	DT	denotes	the
T8774	40211-40217	JJ	denotes	murine
T8773	40218-40231	NN	denotes	trophectoderm
T8775	40231-40233	,	denotes	,
T8776	40233-40235	IN	denotes	as
T8777	40236-40237	DT	denotes	a
T8778	40238-40244	NN	denotes	result
T8779	40245-40247	IN	denotes	of
T8780	40248-40251	DT	denotes	the
T8782	40252-40257	JJ	denotes	early
T8781	40258-40267	NN	denotes	lethality
T8783	40268-40276	VBN	denotes	observed
T8784	40277-40279	IN	denotes	in
T8785	40280-40288	JJ	denotes	Atrxnull
T8786	40289-40296	NNS	denotes	embryos
T8787	40297-40299	PRP	denotes	it
T8765	40300-40302	VBZ	denotes	is
T8788	40303-40306	RB	denotes	not
T8789	40307-40315	JJ	denotes	possible
T8790	40316-40318	TO	denotes	to
T8791	40319-40323	VB	denotes	rule
T8792	40324-40327	RP	denotes	out
T8793	40328-40333	JJ	denotes	other
T8794	40334-40339	NNS	denotes	roles
T8795	40340-40343	IN	denotes	for
T8796	40344-40348	NN	denotes	Atrx
T8797	40349-40351	IN	denotes	at
T8798	40352-40357	JJ	denotes	later
T8800	40358-40371	JJ	denotes	developmental
T8799	40372-40378	NNS	denotes	stages
T8801	40379-40381	IN	denotes	in
T8802	40382-40389	NNS	denotes	tissues
T8803	40390-40392	IN	denotes	of
T8804	40393-40396	DT	denotes	the
T8805	40397-40403	NN	denotes	embryo
T8806	40404-40410	JJ	denotes	proper
T8807	40410-40411	.	denotes	.
T8808	40411-40622	sentence	denotes	Indeed, we show that Atrx is highly expressed throughout the entire developing embryo at 7.5 dpc (Figure 5B), and it is likely that Atrx function will turn out to be important for other differentiating tissues.
T8809	40412-40418	RB	denotes	Indeed
T8811	40418-40420	,	denotes	,
T8812	40420-40422	PRP	denotes	we
T8810	40423-40427	VBP	denotes	show
T8813	40428-40432	IN	denotes	that
T8815	40433-40437	NN	denotes	Atrx
T8814	40438-40440	VBZ	denotes	is
T8816	40441-40447	RB	denotes	highly
T8817	40448-40457	JJ	denotes	expressed
T8818	40458-40468	IN	denotes	throughout
T8819	40469-40472	DT	denotes	the
T8821	40473-40479	JJ	denotes	entire
T8822	40480-40490	VBG	denotes	developing
T8820	40491-40497	NN	denotes	embryo
T8823	40498-40500	IN	denotes	at
T8824	40501-40504	CD	denotes	7.5
T8825	40505-40508	NN	denotes	dpc
T8826	40509-40510	-LRB-	denotes	(
T8828	40510-40516	NN	denotes	Figure
T8827	40517-40519	NN	denotes	5B
T8829	40519-40520	-RRB-	denotes	)
T8830	40520-40522	,	denotes	,
T8831	40522-40525	CC	denotes	and
T8832	40526-40528	PRP	denotes	it
T8833	40529-40531	VBZ	denotes	is
T8834	40532-40538	JJ	denotes	likely
T8835	40539-40543	IN	denotes	that
T8837	40544-40548	NN	denotes	Atrx
T8838	40549-40557	NN	denotes	function
T8839	40558-40562	MD	denotes	will
T8836	40563-40567	VB	denotes	turn
T8840	40568-40571	RP	denotes	out
T8841	40572-40574	TO	denotes	to
T8842	40575-40577	VB	denotes	be
T8843	40578-40587	JJ	denotes	important
T8844	40588-40591	IN	denotes	for
T8845	40592-40597	JJ	denotes	other
T8847	40598-40613	VBG	denotes	differentiating
T8846	40614-40621	NNS	denotes	tissues
T8848	40621-40622	.	denotes	.
T8849	40622-40969	sentence	denotes	Tetraploid aggregation experiments (in which mutant embryos are rescued with wild-type extraembryonic tissues) might shed more light on the role of Atrx during later mouse development, but these issues can be more subtly dissected by combining the conditional Atrx flox allele that we have generated with different tissue-specific Cre transgenes.
T8850	40623-40633	NN	denotes	Tetraploid
T8851	40634-40645	NN	denotes	aggregation
T8852	40646-40657	NNS	denotes	experiments
T8854	40658-40659	-LRB-	denotes	(
T8855	40659-40661	IN	denotes	in
T8857	40662-40667	WDT	denotes	which
T8858	40668-40674	NN	denotes	mutant
T8859	40675-40682	NNS	denotes	embryos
T8860	40683-40686	VBP	denotes	are
T8856	40687-40694	VBN	denotes	rescued
T8861	40695-40699	IN	denotes	with
T8862	40700-40704	JJ	denotes	wild
T8864	40704-40705	HYPH	denotes	-
T8863	40705-40709	NN	denotes	type
T8866	40710-40724	JJ	denotes	extraembryonic
T8865	40725-40732	NNS	denotes	tissues
T8867	40732-40733	-RRB-	denotes	)
T8868	40734-40739	MD	denotes	might
T8853	40740-40744	VB	denotes	shed
T8869	40745-40749	JJR	denotes	more
T8870	40750-40755	NN	denotes	light
T8871	40756-40758	IN	denotes	on
T8872	40759-40762	DT	denotes	the
T8873	40763-40767	NN	denotes	role
T8874	40768-40770	IN	denotes	of
T8875	40771-40775	NN	denotes	Atrx
T8876	40776-40782	IN	denotes	during
T8877	40783-40788	JJ	denotes	later
T8879	40789-40794	NN	denotes	mouse
T8878	40795-40806	NN	denotes	development
T8880	40806-40808	,	denotes	,
T8881	40808-40811	CC	denotes	but
T8882	40812-40817	DT	denotes	these
T8883	40818-40824	NNS	denotes	issues
T8885	40825-40828	MD	denotes	can
T8886	40829-40831	VB	denotes	be
T8887	40832-40836	RBR	denotes	more
T8888	40837-40843	RB	denotes	subtly
T8884	40844-40853	VBN	denotes	dissected
T8889	40854-40856	IN	denotes	by
T8890	40857-40866	VBG	denotes	combining
T8891	40867-40870	DT	denotes	the
T8893	40871-40882	JJ	denotes	conditional
T8894	40883-40892	NN	denotes	Atrx flox
T8892	40893-40899	NN	denotes	allele
T8895	40900-40904	WDT	denotes	that
T8897	40905-40907	PRP	denotes	we
T8898	40908-40912	VBP	denotes	have
T8896	40913-40922	VBN	denotes	generated
T8899	40923-40927	IN	denotes	with
T8900	40928-40937	JJ	denotes	different
T8902	40938-40944	NN	denotes	tissue
T8904	40944-40945	HYPH	denotes	-
T8903	40945-40953	JJ	denotes	specific
T8905	40954-40957	NN	denotes	Cre
T8901	40958-40968	NNS	denotes	transgenes
T8906	40968-40969	.	denotes	.
T8907	40969-41101	sentence	denotes	As mentioned above, this approach has already revealed a critical role for Atrx during neuronal differentiation in adult mice [20].
T8908	40970-40972	IN	denotes	As
T8909	40973-40982	VBN	denotes	mentioned
T8911	40983-40988	RB	denotes	above
T8912	40988-40990	,	denotes	,
T8913	40990-40994	DT	denotes	this
T8914	40995-41003	NN	denotes	approach
T8915	41004-41007	VBZ	denotes	has
T8916	41008-41015	RB	denotes	already
T8910	41016-41024	VBN	denotes	revealed
T8917	41025-41026	DT	denotes	a
T8919	41027-41035	JJ	denotes	critical
T8918	41036-41040	NN	denotes	role
T8920	41041-41044	IN	denotes	for
T8921	41045-41049	NN	denotes	Atrx
T8922	41050-41056	IN	denotes	during
T8923	41057-41065	JJ	denotes	neuronal
T8924	41066-41081	NN	denotes	differentiation
T8925	41082-41084	IN	denotes	in
T8926	41085-41090	JJ	denotes	adult
T8927	41091-41095	NNS	denotes	mice
T8928	41096-41097	-LRB-	denotes	[
T8929	41097-41099	CD	denotes	20
T8930	41099-41100	-RRB-	denotes	]
T8931	41100-41101	.	denotes	.
T8932	41101-41459	sentence	denotes	Further evidence that Atrx is also required at later stages of mouse development is provided by the observed dramatic skewing against Atrx-negative cells in some somatic tissues of carrier female mice, whose tissues initially comprise a mosaic of Atrx-positive and Atrx-negative cells as a result of random X-inactivation (M. Muers, personal communication).
T8933	41102-41109	JJ	denotes	Further
T8934	41110-41118	NN	denotes	evidence
T8936	41119-41123	IN	denotes	that
T8938	41124-41128	NN	denotes	Atrx
T8939	41129-41131	VBZ	denotes	is
T8940	41132-41136	RB	denotes	also
T8937	41137-41145	VBN	denotes	required
T8941	41146-41148	IN	denotes	at
T8942	41149-41154	JJ	denotes	later
T8943	41155-41161	NNS	denotes	stages
T8944	41162-41164	IN	denotes	of
T8945	41165-41170	NN	denotes	mouse
T8946	41171-41182	NN	denotes	development
T8947	41183-41185	VBZ	denotes	is
T8935	41186-41194	VBN	denotes	provided
T8948	41195-41197	IN	denotes	by
T8949	41198-41201	DT	denotes	the
T8951	41202-41210	VBN	denotes	observed
T8952	41211-41219	JJ	denotes	dramatic
T8950	41220-41227	NN	denotes	skewing
T8953	41228-41235	IN	denotes	against
T8954	41236-41240	NN	denotes	Atrx
T8956	41240-41241	HYPH	denotes	-
T8957	41241-41249	JJ	denotes	negative
T8955	41250-41255	NNS	denotes	cells
T8958	41256-41258	IN	denotes	in
T8959	41259-41263	DT	denotes	some
T8961	41264-41271	JJ	denotes	somatic
T8960	41272-41279	NNS	denotes	tissues
T8962	41280-41282	IN	denotes	of
T8963	41283-41290	NN	denotes	carrier
T8965	41291-41297	JJ	denotes	female
T8964	41298-41302	NNS	denotes	mice
T8966	41302-41304	,	denotes	,
T8967	41304-41309	WP$	denotes	whose
T8968	41310-41317	NNS	denotes	tissues
T8970	41318-41327	RB	denotes	initially
T8969	41328-41336	VBP	denotes	comprise
T8971	41337-41338	DT	denotes	a
T8972	41339-41345	NN	denotes	mosaic
T8973	41346-41348	IN	denotes	of
T8974	41349-41353	NN	denotes	Atrx
T8976	41353-41354	HYPH	denotes	-
T8975	41354-41362	JJ	denotes	positive
T8978	41363-41366	CC	denotes	and
T8979	41367-41371	NN	denotes	Atrx
T8981	41371-41372	HYPH	denotes	-
T8980	41372-41380	JJ	denotes	negative
T8977	41381-41386	NNS	denotes	cells
T8982	41387-41389	IN	denotes	as
T8983	41390-41391	DT	denotes	a
T8984	41392-41398	NN	denotes	result
T8985	41399-41401	IN	denotes	of
T8986	41402-41408	JJ	denotes	random
T8988	41409-41410	NN	denotes	X
T8989	41410-41411	HYPH	denotes	-
T8987	41411-41423	NN	denotes	inactivation
T8990	41424-41425	-LRB-	denotes	(
T8991	41425-41427	NNP	denotes	M.
T8992	41428-41433	NNP	denotes	Muers
T8993	41433-41435	,	denotes	,
T8994	41435-41443	JJ	denotes	personal
T8995	41444-41457	NN	denotes	communication
T8996	41457-41458	-RRB-	denotes	)
T8997	41458-41459	.	denotes	.
T8998	41459-41646	sentence	denotes	Atrx joins an expanding list of mouse genes for which targeted disruption results in peri-implantation lethality as a result of trophoblast or placental abnormalities (reviewed in [25]).
T8999	41460-41464	NN	denotes	Atrx
T9000	41465-41470	VBZ	denotes	joins
T9001	41471-41473	DT	denotes	an
T9003	41474-41483	VBG	denotes	expanding
T9002	41484-41488	NN	denotes	list
T9004	41489-41491	IN	denotes	of
T9005	41492-41497	NN	denotes	mouse
T9006	41498-41503	NNS	denotes	genes
T9007	41504-41507	IN	denotes	for
T9009	41508-41513	WDT	denotes	which
T9010	41514-41522	VBN	denotes	targeted
T9011	41523-41533	NN	denotes	disruption
T9008	41534-41541	VBZ	denotes	results
T9012	41542-41544	IN	denotes	in
T9013	41545-41562	JJ	denotes	peri-implantation
T9014	41563-41572	NN	denotes	lethality
T9015	41573-41575	IN	denotes	as
T9016	41576-41577	DT	denotes	a
T9017	41578-41584	NN	denotes	result
T9018	41585-41587	IN	denotes	of
T9019	41588-41599	NN	denotes	trophoblast
T9021	41600-41602	CC	denotes	or
T9022	41603-41612	JJ	denotes	placental
T9020	41613-41626	NNS	denotes	abnormalities
T9023	41627-41628	-LRB-	denotes	(
T9024	41628-41636	VBN	denotes	reviewed
T9025	41637-41639	IN	denotes	in
T9026	41640-41641	-LRB-	denotes	[
T9027	41641-41643	CD	denotes	25
T9028	41643-41644	-RRB-	denotes	]
T9029	41644-41645	-RRB-	denotes	)
T9030	41645-41646	.	denotes	.
T9031	41646-41756	sentence	denotes	Comparison with other phenotypes might provide some insight into the role of Atrx in trophoblast development.
T9032	41647-41657	NN	denotes	Comparison
T9034	41658-41662	IN	denotes	with
T9035	41663-41668	JJ	denotes	other
T9036	41669-41679	NNS	denotes	phenotypes
T9037	41680-41685	MD	denotes	might
T9033	41686-41693	VB	denotes	provide
T9038	41694-41698	DT	denotes	some
T9039	41699-41706	NN	denotes	insight
T9040	41707-41711	IN	denotes	into
T9041	41712-41715	DT	denotes	the
T9042	41716-41720	NN	denotes	role
T9043	41721-41723	IN	denotes	of
T9044	41724-41728	NN	denotes	Atrx
T9045	41729-41731	IN	denotes	in
T9046	41732-41743	NN	denotes	trophoblast
T9047	41744-41755	NN	denotes	development
T9048	41755-41756	.	denotes	.
T9049	41756-41972	sentence	denotes	Atrx-mutant embryos progress further than embryos nullizygous for factors involved in the initial specification of trophoblast stem cells (such as Cdx2) or in stem cell maintenance and proliferation (such as Eomes).
T9050	41757-41761	NN	denotes	Atrx
T9052	41761-41762	HYPH	denotes	-
T9051	41762-41768	NN	denotes	mutant
T9053	41769-41776	NNS	denotes	embryos
T9054	41777-41785	VBP	denotes	progress
T9055	41786-41793	RB	denotes	further
T9056	41794-41798	IN	denotes	than
T9057	41799-41806	NNS	denotes	embryos
T9058	41807-41818	JJ	denotes	nullizygous
T9059	41819-41822	IN	denotes	for
T9060	41823-41830	NNS	denotes	factors
T9061	41831-41839	VBN	denotes	involved
T9062	41840-41842	IN	denotes	in
T9063	41843-41846	DT	denotes	the
T9065	41847-41854	JJ	denotes	initial
T9064	41855-41868	NN	denotes	specification
T9066	41869-41871	IN	denotes	of
T9067	41872-41883	NN	denotes	trophoblast
T9069	41884-41888	NN	denotes	stem
T9068	41889-41894	NNS	denotes	cells
T9070	41895-41896	-LRB-	denotes	(
T9072	41896-41900	JJ	denotes	such
T9071	41901-41903	IN	denotes	as
T9073	41904-41908	NN	denotes	Cdx2
T9074	41908-41909	-RRB-	denotes	)
T9075	41910-41912	CC	denotes	or
T9076	41913-41915	IN	denotes	in
T9077	41916-41920	NN	denotes	stem
T9079	41921-41925	NN	denotes	cell
T9078	41926-41937	NN	denotes	maintenance
T9080	41938-41941	CC	denotes	and
T9081	41942-41955	NN	denotes	proliferation
T9082	41956-41957	-LRB-	denotes	(
T9084	41957-41961	JJ	denotes	such
T9083	41962-41964	IN	denotes	as
T9085	41965-41970	NN	denotes	Eomes
T9086	41970-41971	-RRB-	denotes	)
T9087	41971-41972	.	denotes	.
T9088	41972-42208	sentence	denotes	Cdx2-mutant embryos fail to implant and die between 3.5 and 5.5 dpc [26], while Eomes-mutant blastocysts implant into the uterus, but arrest soon after implantation without forming organised embryonic or extraembryonic structures [27].
T9089	41973-41977	NN	denotes	Cdx2
T9091	41977-41978	HYPH	denotes	-
T9090	41978-41984	NN	denotes	mutant
T9092	41985-41992	NNS	denotes	embryos
T9093	41993-41997	VBP	denotes	fail
T9094	41998-42000	TO	denotes	to
T9095	42001-42008	VB	denotes	implant
T9096	42009-42012	CC	denotes	and
T9097	42013-42016	VBP	denotes	die
T9098	42017-42024	IN	denotes	between
T9099	42025-42028	CD	denotes	3.5
T9101	42029-42032	CC	denotes	and
T9102	42033-42036	CD	denotes	5.5
T9100	42037-42040	NN	denotes	dpc
T9103	42041-42042	-LRB-	denotes	[
T9104	42042-42044	CD	denotes	26
T9105	42044-42045	-RRB-	denotes	]
T9106	42045-42047	,	denotes	,
T9107	42047-42052	IN	denotes	while
T9109	42053-42058	NN	denotes	Eomes
T9111	42058-42059	HYPH	denotes	-
T9110	42059-42065	NN	denotes	mutant
T9112	42066-42077	NNS	denotes	blastocysts
T9108	42078-42085	VBP	denotes	implant
T9113	42086-42090	IN	denotes	into
T9114	42091-42094	DT	denotes	the
T9115	42095-42101	NN	denotes	uterus
T9116	42101-42103	,	denotes	,
T9117	42103-42106	CC	denotes	but
T9118	42107-42113	VBP	denotes	arrest
T9119	42114-42118	RB	denotes	soon
T9120	42119-42124	IN	denotes	after
T9121	42125-42137	NN	denotes	implantation
T9122	42138-42145	IN	denotes	without
T9123	42146-42153	VBG	denotes	forming
T9124	42154-42163	VBN	denotes	organised
T9126	42164-42173	JJ	denotes	embryonic
T9127	42174-42176	CC	denotes	or
T9128	42177-42191	JJ	denotes	extraembryonic
T9125	42192-42202	NNS	denotes	structures
T9129	42203-42204	-LRB-	denotes	[
T9130	42204-42206	CD	denotes	27
T9131	42206-42207	-RRB-	denotes	]
T9132	42207-42208	.	denotes	.
T9133	42208-42319	sentence	denotes	In contrast, Atrx-mutant embryos implant successfully and establish organised embryonic structures by 7.5 dpc.
T9134	42209-42211	IN	denotes	In
T9136	42212-42220	NN	denotes	contrast
T9137	42220-42222	,	denotes	,
T9138	42222-42226	NN	denotes	Atrx
T9140	42226-42227	HYPH	denotes	-
T9139	42227-42233	NN	denotes	mutant
T9141	42234-42241	NNS	denotes	embryos
T9135	42242-42249	VBP	denotes	implant
T9142	42250-42262	RB	denotes	successfully
T9143	42263-42266	CC	denotes	and
T9144	42267-42276	VBP	denotes	establish
T9145	42277-42286	VBN	denotes	organised
T9147	42287-42296	JJ	denotes	embryonic
T9146	42297-42307	NNS	denotes	structures
T9148	42308-42310	IN	denotes	by
T9149	42311-42314	CD	denotes	7.5
T9150	42315-42318	NN	denotes	dpc
T9151	42318-42319	.	denotes	.
T9152	42319-42456	sentence	denotes	The Atrx-mutant phenotype closely resembles that observed in mice nullizygous for the basic helix-loop-helix transcription factor Hand1.
T9153	42320-42323	DT	denotes	The
T9155	42324-42328	NN	denotes	Atrx
T9157	42328-42329	HYPH	denotes	-
T9156	42329-42335	NN	denotes	mutant
T9154	42336-42345	NN	denotes	phenotype
T9159	42346-42353	RB	denotes	closely
T9158	42354-42363	VBZ	denotes	resembles
T9160	42364-42368	DT	denotes	that
T9161	42369-42377	VBN	denotes	observed
T9162	42378-42380	IN	denotes	in
T9163	42381-42385	NNS	denotes	mice
T9164	42386-42397	JJ	denotes	nullizygous
T9165	42398-42401	IN	denotes	for
T9166	42402-42405	DT	denotes	the
T9168	42406-42411	JJ	denotes	basic
T9169	42412-42417	NN	denotes	helix
T9171	42417-42418	HYPH	denotes	-
T9172	42418-42422	NN	denotes	loop
T9173	42422-42423	HYPH	denotes	-
T9170	42423-42428	NN	denotes	helix
T9174	42429-42442	NN	denotes	transcription
T9167	42443-42449	NN	denotes	factor
T9175	42450-42455	NN	denotes	Hand1
T9176	42455-42456	.	denotes	.
T9177	42456-42682	sentence	denotes	Hand1-mutant conceptuses arrest at around 7.5 dpc and display a normal embryonic compartment, but, like Atrx-mutant embryos, ablation of Hand1 causes a reduction in the size of the ectoplacental cone and density of TGCs [28].
T9178	42457-42462	NN	denotes	Hand1
T9180	42462-42463	HYPH	denotes	-
T9179	42463-42469	NN	denotes	mutant
T9181	42470-42481	NNS	denotes	conceptuses
T9182	42482-42488	VBP	denotes	arrest
T9183	42489-42491	IN	denotes	at
T9184	42492-42498	IN	denotes	around
T9185	42499-42502	CD	denotes	7.5
T9186	42503-42506	NN	denotes	dpc
T9187	42507-42510	CC	denotes	and
T9188	42511-42518	VBP	denotes	display
T9189	42519-42520	DT	denotes	a
T9191	42521-42527	JJ	denotes	normal
T9192	42528-42537	JJ	denotes	embryonic
T9190	42538-42549	NN	denotes	compartment
T9193	42549-42551	,	denotes	,
T9194	42551-42554	CC	denotes	but
T9195	42554-42556	,	denotes	,
T9196	42556-42560	IN	denotes	like
T9198	42561-42565	NN	denotes	Atrx
T9200	42565-42566	HYPH	denotes	-
T9199	42566-42572	NN	denotes	mutant
T9201	42573-42580	NNS	denotes	embryos
T9202	42580-42582	,	denotes	,
T9203	42582-42590	NN	denotes	ablation
T9204	42591-42593	IN	denotes	of
T9205	42594-42599	NN	denotes	Hand1
T9197	42600-42606	VBZ	denotes	causes
T9206	42607-42608	DT	denotes	a
T9207	42609-42618	NN	denotes	reduction
T9208	42619-42621	IN	denotes	in
T9209	42622-42625	DT	denotes	the
T9210	42626-42630	NN	denotes	size
T9211	42631-42633	IN	denotes	of
T9212	42634-42637	DT	denotes	the
T9214	42638-42651	JJ	denotes	ectoplacental
T9213	42652-42656	NN	denotes	cone
T9215	42657-42660	CC	denotes	and
T9216	42661-42668	NN	denotes	density
T9217	42669-42671	IN	denotes	of
T9218	42672-42676	NNS	denotes	TGCs
T9219	42677-42678	-LRB-	denotes	[
T9220	42678-42680	CD	denotes	28
T9221	42680-42681	-RRB-	denotes	]
T9222	42681-42682	.	denotes	.
T9223	42682-42785	sentence	denotes	As with Atrx mutants, only arrested or resorbed Hand1-mutant conceptuses were recovered after 8.5 dpc.
T9224	42683-42685	IN	denotes	As
T9226	42686-42690	IN	denotes	with
T9227	42691-42695	NN	denotes	Atrx
T9228	42696-42703	NNS	denotes	mutants
T9229	42703-42705	,	denotes	,
T9230	42705-42709	RB	denotes	only
T9232	42710-42718	VBN	denotes	arrested
T9233	42719-42721	CC	denotes	or
T9234	42722-42730	VBN	denotes	resorbed
T9235	42731-42736	NN	denotes	Hand1
T9237	42736-42737	HYPH	denotes	-
T9236	42737-42743	NN	denotes	mutant
T9231	42744-42755	NNS	denotes	conceptuses
T9238	42756-42760	VBD	denotes	were
T9225	42761-42770	VBN	denotes	recovered
T9239	42771-42776	IN	denotes	after
T9240	42777-42780	CD	denotes	8.5
T9241	42781-42784	NN	denotes	dpc
T9242	42784-42785	.	denotes	.
T9243	42785-42943	sentence	denotes	Also like Atrx, disruption of Hand1 specifically affects secondary giant cell formation, and primary trophoblast outgrowths from blastocysts appeared normal.
T9244	42786-42790	RB	denotes	Also
T9246	42791-42795	IN	denotes	like
T9247	42796-42800	NN	denotes	Atrx
T9248	42800-42802	,	denotes	,
T9249	42802-42812	NN	denotes	disruption
T9250	42813-42815	IN	denotes	of
T9251	42816-42821	NN	denotes	Hand1
T9252	42822-42834	RB	denotes	specifically
T9245	42835-42842	VBZ	denotes	affects
T9253	42843-42852	JJ	denotes	secondary
T9255	42853-42858	JJ	denotes	giant
T9254	42859-42863	NN	denotes	cell
T9256	42864-42873	NN	denotes	formation
T9257	42873-42875	,	denotes	,
T9258	42875-42878	CC	denotes	and
T9259	42879-42886	JJ	denotes	primary
T9261	42887-42898	NN	denotes	trophoblast
T9260	42899-42909	NNS	denotes	outgrowths
T9263	42910-42914	IN	denotes	from
T9264	42915-42926	NNS	denotes	blastocysts
T9262	42927-42935	VBD	denotes	appeared
T9265	42936-42942	JJ	denotes	normal
T9266	42942-42943	.	denotes	.
T9267	42943-43109	sentence	denotes	Hand1 is required for terminal differentiation of secondary TGCs, and in its absence trophoblast cells arrest at a precursor stage in the ectoplacental cone [17,28].
T9268	42944-42949	NN	denotes	Hand1
T9270	42950-42952	VBZ	denotes	is
T9269	42953-42961	VBN	denotes	required
T9271	42962-42965	IN	denotes	for
T9272	42966-42974	JJ	denotes	terminal
T9273	42975-42990	NN	denotes	differentiation
T9274	42991-42993	IN	denotes	of
T9275	42994-43003	JJ	denotes	secondary
T9276	43004-43008	NNS	denotes	TGCs
T9277	43008-43010	,	denotes	,
T9278	43010-43013	CC	denotes	and
T9279	43014-43016	IN	denotes	in
T9281	43017-43020	PRP$	denotes	its
T9282	43021-43028	NN	denotes	absence
T9283	43029-43040	NN	denotes	trophoblast
T9284	43041-43046	NNS	denotes	cells
T9280	43047-43053	VBP	denotes	arrest
T9285	43054-43056	IN	denotes	at
T9286	43057-43058	DT	denotes	a
T9288	43059-43068	NN	denotes	precursor
T9287	43069-43074	NN	denotes	stage
T9289	43075-43077	IN	denotes	in
T9290	43078-43081	DT	denotes	the
T9292	43082-43095	JJ	denotes	ectoplacental
T9291	43096-43100	NN	denotes	cone
T9293	43101-43102	-LRB-	denotes	[
T9295	43102-43104	CD	denotes	17
T9296	43104-43105	,	denotes	,
T9294	43105-43107	CD	denotes	28
T9297	43107-43108	-RRB-	denotes	]
T9298	43108-43109	.	denotes	.
T9299	43109-43480	sentence	denotes	Given the similarity of the Atrx- and Hand1-mutant phenotypes and the likelihood that Atrx acts as a transcriptional regulator by modifying chromatin structure, it will be of interest to determine whether Atrx is itself a regulator of Hand1 expression, or alternatively whether it acts as a co-regulator of one or more of the downstream transcriptional targets of Hand1.
T9300	43110-43115	VBN	denotes	Given
T9302	43116-43119	DT	denotes	the
T9303	43120-43130	NN	denotes	similarity
T9304	43131-43133	IN	denotes	of
T9305	43134-43137	DT	denotes	the
T9307	43138-43142	NN	denotes	Atrx
T9309	43142-43143	HYPH	denotes	-
T9310	43144-43147	CC	denotes	and
T9311	43148-43153	NN	denotes	Hand1
T9312	43153-43154	HYPH	denotes	-
T9308	43154-43160	NN	denotes	mutant
T9306	43161-43171	NNS	denotes	phenotypes
T9313	43172-43175	CC	denotes	and
T9314	43176-43179	DT	denotes	the
T9315	43180-43190	NN	denotes	likelihood
T9316	43191-43195	IN	denotes	that
T9318	43196-43200	NN	denotes	Atrx
T9317	43201-43205	VBZ	denotes	acts
T9319	43206-43208	IN	denotes	as
T9320	43209-43210	DT	denotes	a
T9322	43211-43226	JJ	denotes	transcriptional
T9321	43227-43236	NN	denotes	regulator
T9323	43237-43239	IN	denotes	by
T9324	43240-43249	VBG	denotes	modifying
T9325	43250-43259	NN	denotes	chromatin
T9326	43260-43269	NN	denotes	structure
T9327	43269-43271	,	denotes	,
T9328	43271-43273	PRP	denotes	it
T9329	43274-43278	MD	denotes	will
T9301	43279-43281	VB	denotes	be
T9330	43282-43284	IN	denotes	of
T9331	43285-43293	NN	denotes	interest
T9332	43294-43296	TO	denotes	to
T9333	43297-43306	VB	denotes	determine
T9334	43307-43314	IN	denotes	whether
T9336	43315-43319	NN	denotes	Atrx
T9335	43320-43322	VBZ	denotes	is
T9337	43323-43329	PRP	denotes	itself
T9338	43330-43331	DT	denotes	a
T9339	43332-43341	NN	denotes	regulator
T9340	43342-43344	IN	denotes	of
T9341	43345-43350	NN	denotes	Hand1
T9342	43351-43361	NN	denotes	expression
T9343	43361-43363	,	denotes	,
T9344	43363-43365	CC	denotes	or
T9345	43366-43379	RB	denotes	alternatively
T9347	43380-43387	IN	denotes	whether
T9348	43388-43390	PRP	denotes	it
T9346	43391-43395	VBZ	denotes	acts
T9349	43396-43398	IN	denotes	as
T9350	43399-43400	DT	denotes	a
T9351	43401-43413	NN	denotes	co-regulator
T9352	43414-43416	IN	denotes	of
T9353	43417-43420	CD	denotes	one
T9354	43421-43423	CC	denotes	or
T9355	43424-43428	JJR	denotes	more
T9356	43429-43431	IN	denotes	of
T9357	43432-43435	DT	denotes	the
T9359	43436-43446	JJ	denotes	downstream
T9360	43447-43462	JJ	denotes	transcriptional
T9358	43463-43470	NNS	denotes	targets
T9361	43471-43473	IN	denotes	of
T9362	43474-43479	NN	denotes	Hand1
T9363	43479-43480	.	denotes	.
T9364	43480-43613	sentence	denotes	It is noteworthy that, in the brain-specific Atrx knockout mice, the defect was observed in terminally differentiating neurons [20].
T9365	43481-43483	PRP	denotes	It
T9366	43484-43486	VBZ	denotes	is
T9367	43487-43497	JJ	denotes	noteworthy
T9368	43498-43502	IN	denotes	that
T9370	43502-43504	,	denotes	,
T9371	43504-43506	IN	denotes	in
T9372	43507-43510	DT	denotes	the
T9374	43511-43516	NN	denotes	brain
T9376	43516-43517	HYPH	denotes	-
T9375	43517-43525	JJ	denotes	specific
T9377	43526-43530	NN	denotes	Atrx
T9378	43531-43539	NN	denotes	knockout
T9373	43540-43544	NNS	denotes	mice
T9379	43544-43546	,	denotes	,
T9380	43546-43549	DT	denotes	the
T9381	43550-43556	NN	denotes	defect
T9382	43557-43560	VBD	denotes	was
T9369	43561-43569	VBN	denotes	observed
T9383	43570-43572	IN	denotes	in
T9384	43573-43583	RB	denotes	terminally
T9385	43584-43599	VBG	denotes	differentiating
T9386	43600-43607	NNS	denotes	neurons
T9387	43608-43609	-LRB-	denotes	[
T9388	43609-43611	CD	denotes	20
T9389	43611-43612	-RRB-	denotes	]
T9390	43612-43613	.	denotes	.
T9391	43613-43922	sentence	denotes	The secondary TGCs affected in the universal Atrx knockout reported here represent one of the first terminally differentiated tissues in the developing mouse, and this may point to the requirement for Atrx in the high-level expression of some tissue-specific genes during the final stages of differentiation.
T9392	43614-43617	DT	denotes	The
T9394	43618-43627	JJ	denotes	secondary
T9393	43628-43632	NNS	denotes	TGCs
T9396	43633-43641	VBN	denotes	affected
T9397	43642-43644	IN	denotes	in
T9398	43645-43648	DT	denotes	the
T9400	43649-43658	JJ	denotes	universal
T9401	43659-43663	NN	denotes	Atrx
T9399	43664-43672	NN	denotes	knockout
T9402	43673-43681	VBN	denotes	reported
T9403	43682-43686	RB	denotes	here
T9395	43687-43696	VBP	denotes	represent
T9404	43697-43700	CD	denotes	one
T9405	43701-43703	IN	denotes	of
T9406	43704-43707	DT	denotes	the
T9408	43708-43713	JJ	denotes	first
T9409	43714-43724	RB	denotes	terminally
T9410	43725-43739	VBN	denotes	differentiated
T9407	43740-43747	NNS	denotes	tissues
T9411	43748-43750	IN	denotes	in
T9412	43751-43754	DT	denotes	the
T9414	43755-43765	VBG	denotes	developing
T9413	43766-43771	NN	denotes	mouse
T9415	43771-43773	,	denotes	,
T9416	43773-43776	CC	denotes	and
T9417	43777-43781	DT	denotes	this
T9419	43782-43785	MD	denotes	may
T9418	43786-43791	VB	denotes	point
T9420	43792-43794	IN	denotes	to
T9421	43795-43798	DT	denotes	the
T9422	43799-43810	NN	denotes	requirement
T9423	43811-43814	IN	denotes	for
T9424	43815-43819	NN	denotes	Atrx
T9425	43820-43822	IN	denotes	in
T9426	43823-43826	DT	denotes	the
T9428	43827-43831	JJ	denotes	high
T9430	43831-43832	HYPH	denotes	-
T9429	43832-43837	NN	denotes	level
T9427	43838-43848	NN	denotes	expression
T9431	43849-43851	IN	denotes	of
T9432	43852-43856	DT	denotes	some
T9434	43857-43863	NN	denotes	tissue
T9436	43863-43864	HYPH	denotes	-
T9435	43864-43872	JJ	denotes	specific
T9433	43873-43878	NNS	denotes	genes
T9437	43879-43885	IN	denotes	during
T9438	43886-43889	DT	denotes	the
T9440	43890-43895	JJ	denotes	final
T9439	43896-43902	NNS	denotes	stages
T9441	43903-43905	IN	denotes	of
T9442	43906-43921	NN	denotes	differentiation
T9443	43921-43922	.	denotes	.
T9444	43922-44130	sentence	denotes	Interestingly, the α-globin genes, the only confirmed transcriptional targets of regulation by human ATRX, are also highly expressed specifically during terminal differentiation within the erythroid lineage.
T9445	43923-43936	RB	denotes	Interestingly
T9447	43936-43938	,	denotes	,
T9448	43938-43941	DT	denotes	the
T9450	43942-43943	NN	denotes	α
T9452	43943-43944	HYPH	denotes	-
T9451	43944-43950	NN	denotes	globin
T9449	43951-43956	NNS	denotes	genes
T9453	43956-43958	,	denotes	,
T9454	43958-43961	DT	denotes	the
T9456	43962-43966	RB	denotes	only
T9457	43967-43976	VBN	denotes	confirmed
T9458	43977-43992	JJ	denotes	transcriptional
T9455	43993-44000	NNS	denotes	targets
T9459	44001-44003	IN	denotes	of
T9460	44004-44014	NN	denotes	regulation
T9461	44015-44017	IN	denotes	by
T9462	44018-44023	JJ	denotes	human
T9463	44024-44028	NN	denotes	ATRX
T9464	44028-44030	,	denotes	,
T9465	44030-44033	VBP	denotes	are
T9466	44034-44038	RB	denotes	also
T9467	44039-44045	RB	denotes	highly
T9446	44046-44055	VBN	denotes	expressed
T9468	44056-44068	RB	denotes	specifically
T9469	44069-44075	IN	denotes	during
T9470	44076-44084	JJ	denotes	terminal
T9471	44085-44100	NN	denotes	differentiation
T9472	44101-44107	IN	denotes	within
T9473	44108-44111	DT	denotes	the
T9475	44112-44121	JJ	denotes	erythroid
T9474	44122-44129	NN	denotes	lineage
T9476	44129-44130	.	denotes	.
T9932	44132-44136	NN	denotes	Atrx
T9933	44137-44144	VBZ	denotes	Escapes
T9934	44145-44154	VBN	denotes	Imprinted
T9936	44155-44156	NN	denotes	X
T9937	44156-44157	HYPH	denotes	-
T9935	44157-44169	NN	denotes	Inactivation
T9938	44170-44172	IN	denotes	in
T9939	44173-44187	JJ	denotes	Extraembryonic
T9940	44188-44195	NNS	denotes	Tissues
T9941	44196-44198	IN	denotes	of
T9942	44199-44206	NN	denotes	Carrier
T9944	44207-44213	JJ	denotes	Female
T9943	44214-44218	NNS	denotes	Mice
T9945	44218-44501	sentence	denotes	Another surprising finding of this study is that, in carrier female embryos, a paternally inherited Atrx WT allele appears to escape the process of imprinted X-inactivation, which ordinarily silences the Xp chromosome in the extraembryonic compartment of female murine tissues [18].
T9946	44219-44226	DT	denotes	Another
T9948	44227-44237	JJ	denotes	surprising
T9947	44238-44245	NN	denotes	finding
T9950	44246-44248	IN	denotes	of
T9951	44249-44253	DT	denotes	this
T9952	44254-44259	NN	denotes	study
T9949	44260-44262	VBZ	denotes	is
T9953	44263-44267	IN	denotes	that
T9955	44267-44269	,	denotes	,
T9956	44269-44271	IN	denotes	in
T9957	44272-44279	NN	denotes	carrier
T9959	44280-44286	JJ	denotes	female
T9958	44287-44294	NNS	denotes	embryos
T9960	44294-44296	,	denotes	,
T9961	44296-44297	DT	denotes	a
T9963	44298-44308	RB	denotes	paternally
T9964	44309-44318	VBN	denotes	inherited
T9965	44319-44326	NN	denotes	Atrx WT
T9962	44327-44333	NN	denotes	allele
T9954	44334-44341	VBZ	denotes	appears
T9966	44342-44344	TO	denotes	to
T9967	44345-44351	VB	denotes	escape
T9968	44352-44355	DT	denotes	the
T9969	44356-44363	NN	denotes	process
T9970	44364-44366	IN	denotes	of
T9971	44367-44376	VBN	denotes	imprinted
T9973	44377-44378	NN	denotes	X
T9974	44378-44379	HYPH	denotes	-
T9972	44379-44391	NN	denotes	inactivation
T9975	44391-44393	,	denotes	,
T9976	44393-44398	WDT	denotes	which
T9978	44399-44409	RB	denotes	ordinarily
T9977	44410-44418	VBZ	denotes	silences
T9979	44419-44422	DT	denotes	the
T9981	44423-44425	NN	denotes	Xp
T9980	44426-44436	NN	denotes	chromosome
T9982	44437-44439	IN	denotes	in
T9983	44440-44443	DT	denotes	the
T9985	44444-44458	JJ	denotes	extraembryonic
T9984	44459-44470	NN	denotes	compartment
T9986	44471-44473	IN	denotes	of
T9987	44474-44480	JJ	denotes	female
T9989	44481-44487	JJ	denotes	murine
T9988	44488-44495	NNS	denotes	tissues
T9990	44496-44497	-LRB-	denotes	[
T9991	44497-44499	CD	denotes	18
T9992	44499-44500	-RRB-	denotes	]
T9993	44500-44501	.	denotes	.
T9994	44501-44685	sentence	denotes	Silencing of the Atrx WT allele on Xp should render these females null for Atrx in the extraembryonic tissues, since the normally active Xm chromosome carries the Atrx Δ18Δneo allele.
T9995	44502-44511	NN	denotes	Silencing
T9997	44512-44514	IN	denotes	of
T9998	44515-44518	DT	denotes	the
T10000	44519-44526	NN	denotes	Atrx WT
T9999	44527-44533	NN	denotes	allele
T10001	44534-44536	IN	denotes	on
T10002	44537-44539	NN	denotes	Xp
T10003	44540-44546	MD	denotes	should
T9996	44547-44553	VB	denotes	render
T10004	44554-44559	DT	denotes	these
T10005	44560-44567	NNS	denotes	females
T10006	44568-44572	JJ	denotes	null
T10007	44573-44576	IN	denotes	for
T10008	44577-44581	NN	denotes	Atrx
T10009	44582-44584	IN	denotes	in
T10010	44585-44588	DT	denotes	the
T10012	44589-44603	JJ	denotes	extraembryonic
T10011	44604-44611	NNS	denotes	tissues
T10013	44611-44613	,	denotes	,
T10014	44613-44618	IN	denotes	since
T10016	44619-44622	DT	denotes	the
T10018	44623-44631	RB	denotes	normally
T10019	44632-44638	JJ	denotes	active
T10020	44639-44641	NN	denotes	Xm
T10017	44642-44652	NN	denotes	chromosome
T10015	44653-44660	VBZ	denotes	carries
T10021	44661-44664	DT	denotes	the
T10023	44665-44677	NN	denotes	Atrx Δ18Δneo
T10022	44678-44684	NN	denotes	allele
T10024	44684-44685	.	denotes	.
T10025	44685-44791	sentence	denotes	Although not phenotypically normal, some Atrx carrier females developed to term and went on to reproduce.
T10026	44686-44694	IN	denotes	Although
T10028	44695-44698	RB	denotes	not
T10029	44699-44713	RB	denotes	phenotypically
T10027	44714-44720	JJ	denotes	normal
T10031	44720-44722	,	denotes	,
T10032	44722-44726	DT	denotes	some
T10034	44727-44731	NN	denotes	Atrx
T10035	44732-44739	NN	denotes	carrier
T10033	44740-44747	NNS	denotes	females
T10030	44748-44757	VBD	denotes	developed
T10036	44758-44760	IN	denotes	to
T10037	44761-44765	NN	denotes	term
T10038	44766-44769	CC	denotes	and
T10039	44770-44774	VBD	denotes	went
T10040	44775-44777	RP	denotes	on
T10041	44778-44780	TO	denotes	to
T10042	44781-44790	VB	denotes	reproduce
T10043	44790-44791	.	denotes	.
T10044	44791-45109	sentence	denotes	Thus, the failure to correctly silence the paternally derived Atrx WT allele in the extraembryonic tissues of carrier females is consistent with our observations that in Atrxnull males, the Atrx protein plays an essential role in the development of the trophoblast and is necessary for survival in utero in the mouse.
T10045	44792-44796	RB	denotes	Thus
T10047	44796-44798	,	denotes	,
T10048	44798-44801	DT	denotes	the
T10049	44802-44809	NN	denotes	failure
T10050	44810-44812	TO	denotes	to
T10052	44813-44822	RB	denotes	correctly
T10051	44823-44830	VB	denotes	silence
T10053	44831-44834	DT	denotes	the
T10055	44835-44845	RB	denotes	paternally
T10056	44846-44853	VBN	denotes	derived
T10057	44854-44861	NN	denotes	Atrx WT
T10054	44862-44868	NN	denotes	allele
T10058	44869-44871	IN	denotes	in
T10059	44872-44875	DT	denotes	the
T10061	44876-44890	JJ	denotes	extraembryonic
T10060	44891-44898	NNS	denotes	tissues
T10062	44899-44901	IN	denotes	of
T10063	44902-44909	NN	denotes	carrier
T10064	44910-44917	NNS	denotes	females
T10046	44918-44920	VBZ	denotes	is
T10065	44921-44931	JJ	denotes	consistent
T10066	44932-44936	IN	denotes	with
T10067	44937-44940	PRP$	denotes	our
T10068	44941-44953	NNS	denotes	observations
T10069	44954-44958	IN	denotes	that
T10071	44959-44961	IN	denotes	in
T10072	44962-44970	JJ	denotes	Atrxnull
T10073	44971-44976	NNS	denotes	males
T10074	44976-44978	,	denotes	,
T10075	44978-44981	DT	denotes	the
T10077	44982-44986	NN	denotes	Atrx
T10076	44987-44994	NN	denotes	protein
T10070	44995-45000	VBZ	denotes	plays
T10078	45001-45003	DT	denotes	an
T10080	45004-45013	JJ	denotes	essential
T10079	45014-45018	NN	denotes	role
T10081	45019-45021	IN	denotes	in
T10082	45022-45025	DT	denotes	the
T10083	45026-45037	NN	denotes	development
T10084	45038-45040	IN	denotes	of
T10085	45041-45044	DT	denotes	the
T10086	45045-45056	NN	denotes	trophoblast
T10087	45057-45060	CC	denotes	and
T10088	45061-45063	VBZ	denotes	is
T10089	45064-45073	JJ	denotes	necessary
T10090	45074-45077	IN	denotes	for
T10091	45078-45086	NN	denotes	survival
T10092	45087-45089	FW	denotes	in
T10093	45090-45095	FW	denotes	utero
T10094	45096-45098	IN	denotes	in
T10095	45099-45102	DT	denotes	the
T10096	45103-45108	NN	denotes	mouse
T10097	45108-45109	.	denotes	.
T10098	45109-45294	sentence	denotes	The survival of Atrx carrier females contrasts with the phenotypes seen in carriers of mutations of other murine X-linked genes known to be essential in the extraembryonic compartment.
T10099	45110-45113	DT	denotes	The
T10100	45114-45122	NN	denotes	survival
T10102	45123-45125	IN	denotes	of
T10103	45126-45130	NN	denotes	Atrx
T10105	45131-45138	NN	denotes	carrier
T10104	45139-45146	NNS	denotes	females
T10101	45147-45156	VBZ	denotes	contrasts
T10106	45157-45161	IN	denotes	with
T10107	45162-45165	DT	denotes	the
T10108	45166-45176	NNS	denotes	phenotypes
T10109	45177-45181	VBN	denotes	seen
T10110	45182-45184	IN	denotes	in
T10111	45185-45193	NNS	denotes	carriers
T10112	45194-45196	IN	denotes	of
T10113	45197-45206	NNS	denotes	mutations
T10114	45207-45209	IN	denotes	of
T10115	45210-45215	JJ	denotes	other
T10117	45216-45222	JJ	denotes	murine
T10118	45223-45224	NN	denotes	X
T10120	45224-45225	HYPH	denotes	-
T10119	45225-45231	VBN	denotes	linked
T10116	45232-45237	NNS	denotes	genes
T10121	45238-45243	VBN	denotes	known
T10122	45244-45246	TO	denotes	to
T10123	45247-45249	VB	denotes	be
T10124	45250-45259	JJ	denotes	essential
T10125	45260-45262	IN	denotes	in
T10126	45263-45266	DT	denotes	the
T10128	45267-45281	JJ	denotes	extraembryonic
T10127	45282-45293	NN	denotes	compartment
T10129	45293-45294	.	denotes	.
T10130	45294-45533	sentence	denotes	For example, targeted disruption of the dyskerin (Dkc1), glucose 6-phosphate dehydrogenase (G6PD), and choroideremia (Chm) genes cause embryonic lethality in null male embryos through defects of the extraembryonic-derived tissues [29–31].
T10131	45295-45298	IN	denotes	For
T10133	45299-45306	NN	denotes	example
T10134	45306-45308	,	denotes	,
T10135	45308-45316	VBN	denotes	targeted
T10136	45317-45327	NN	denotes	disruption
T10137	45328-45330	IN	denotes	of
T10138	45331-45334	DT	denotes	the
T10140	45335-45343	NN	denotes	dyskerin
T10141	45344-45345	-LRB-	denotes	(
T10142	45345-45349	NN	denotes	Dkc1
T10143	45349-45350	-RRB-	denotes	)
T10144	45350-45352	,	denotes	,
T10145	45352-45359	NN	denotes	glucose
T10147	45360-45361	CD	denotes	6
T10148	45361-45362	HYPH	denotes	-
T10146	45362-45371	NN	denotes	phosphate
T10149	45372-45385	NN	denotes	dehydrogenase
T10150	45386-45387	-LRB-	denotes	(
T10151	45387-45391	NN	denotes	G6PD
T10152	45391-45392	-RRB-	denotes	)
T10153	45392-45394	,	denotes	,
T10154	45394-45397	CC	denotes	and
T10155	45398-45411	NN	denotes	choroideremia
T10156	45412-45413	-LRB-	denotes	(
T10157	45413-45416	NN	denotes	Chm
T10158	45416-45417	-RRB-	denotes	)
T10139	45418-45423	NNS	denotes	genes
T10132	45424-45429	VBP	denotes	cause
T10159	45430-45439	JJ	denotes	embryonic
T10160	45440-45449	NN	denotes	lethality
T10161	45450-45452	IN	denotes	in
T10162	45453-45457	JJ	denotes	null
T10164	45458-45462	JJ	denotes	male
T10163	45463-45470	NNS	denotes	embryos
T10165	45471-45478	IN	denotes	through
T10166	45479-45486	NNS	denotes	defects
T10167	45487-45489	IN	denotes	of
T10168	45490-45493	DT	denotes	the
T10170	45494-45508	JJ	denotes	extraembryonic
T10172	45508-45509	HYPH	denotes	-
T10171	45509-45516	VBN	denotes	derived
T10169	45517-45524	NNS	denotes	tissues
T10173	45525-45526	-LRB-	denotes	[
T10174	45526-45528	CD	denotes	29
T10175	45528-45529	SYM	denotes	–
T10176	45529-45531	CD	denotes	31
T10177	45531-45532	-RRB-	denotes	]
T10178	45532-45533	.	denotes	.
T10179	45533-45711	sentence	denotes	Female mice carrying mutations of these genes on the maternally inherited X chromosome also die in utero, whereas females that inherit the mutation on the Xp chromosome survive.
T10180	45534-45540	JJ	denotes	Female
T10181	45541-45545	NNS	denotes	mice
T10183	45546-45554	VBG	denotes	carrying
T10184	45555-45564	NNS	denotes	mutations
T10185	45565-45567	IN	denotes	of
T10186	45568-45573	DT	denotes	these
T10187	45574-45579	NNS	denotes	genes
T10188	45580-45582	IN	denotes	on
T10189	45583-45586	DT	denotes	the
T10191	45587-45597	RB	denotes	maternally
T10192	45598-45607	VBN	denotes	inherited
T10193	45608-45609	NN	denotes	X
T10190	45610-45620	NN	denotes	chromosome
T10194	45621-45625	RB	denotes	also
T10182	45626-45629	VBP	denotes	die
T10195	45630-45632	FW	denotes	in
T10196	45633-45638	FW	denotes	utero
T10197	45638-45640	,	denotes	,
T10198	45640-45647	IN	denotes	whereas
T10200	45648-45655	NNS	denotes	females
T10201	45656-45660	WDT	denotes	that
T10202	45661-45668	VBP	denotes	inherit
T10203	45669-45672	DT	denotes	the
T10204	45673-45681	NN	denotes	mutation
T10205	45682-45684	IN	denotes	on
T10206	45685-45688	DT	denotes	the
T10208	45689-45691	NN	denotes	Xp
T10207	45692-45702	NN	denotes	chromosome
T10199	45703-45710	VBP	denotes	survive
T10209	45710-45711	.	denotes	.
T10210	45711-45932	sentence	denotes	Thus, unlike Atrx, these genes and/or their effects on cell growth are unable to circumvent the processes that ultimately cause all cells in the extraembryonic tissues to express only the maternally derived X chromosome.
T10211	45712-45716	RB	denotes	Thus
T10213	45716-45718	,	denotes	,
T10214	45718-45724	IN	denotes	unlike
T10215	45725-45729	NN	denotes	Atrx
T10216	45729-45731	,	denotes	,
T10217	45731-45736	DT	denotes	these
T10218	45737-45742	NNS	denotes	genes
T10219	45743-45746	CC	denotes	and
T10220	45746-45747	HYPH	denotes	/
T10221	45747-45749	CC	denotes	or
T10222	45750-45755	PRP$	denotes	their
T10223	45756-45763	NNS	denotes	effects
T10224	45764-45766	IN	denotes	on
T10225	45767-45771	NN	denotes	cell
T10226	45772-45778	NN	denotes	growth
T10212	45779-45782	VBP	denotes	are
T10227	45783-45789	JJ	denotes	unable
T10228	45790-45792	TO	denotes	to
T10229	45793-45803	VB	denotes	circumvent
T10230	45804-45807	DT	denotes	the
T10231	45808-45817	NNS	denotes	processes
T10232	45818-45822	WDT	denotes	that
T10234	45823-45833	RB	denotes	ultimately
T10233	45834-45839	VBP	denotes	cause
T10235	45840-45843	DT	denotes	all
T10236	45844-45849	NNS	denotes	cells
T10238	45850-45852	IN	denotes	in
T10239	45853-45856	DT	denotes	the
T10241	45857-45871	JJ	denotes	extraembryonic
T10240	45872-45879	NNS	denotes	tissues
T10242	45880-45882	TO	denotes	to
T10237	45883-45890	VB	denotes	express
T10243	45891-45895	RB	denotes	only
T10245	45896-45899	DT	denotes	the
T10246	45900-45910	RB	denotes	maternally
T10247	45911-45918	VBN	denotes	derived
T10248	45919-45920	NN	denotes	X
T10244	45921-45931	NN	denotes	chromosome
T10249	45931-45932	.	denotes	.
T10250	45932-46057	sentence	denotes	How might expression of the paternal Atrx WT allele be maintained in the extraembryonic tissues of the Atrx carrier females?
T10251	45933-45936	WRB	denotes	How
T10253	45937-45942	MD	denotes	might
T10254	45943-45953	NN	denotes	expression
T10255	45954-45956	IN	denotes	of
T10256	45957-45960	DT	denotes	the
T10258	45961-45969	JJ	denotes	paternal
T10259	45970-45977	NN	denotes	Atrx WT
T10257	45978-45984	NN	denotes	allele
T10260	45985-45987	VB	denotes	be
T10252	45988-45998	VBN	denotes	maintained
T10261	45999-46001	IN	denotes	in
T10262	46002-46005	DT	denotes	the
T10264	46006-46020	JJ	denotes	extraembryonic
T10263	46021-46028	NNS	denotes	tissues
T10265	46029-46031	IN	denotes	of
T10266	46032-46035	DT	denotes	the
T10268	46036-46040	NN	denotes	Atrx
T10269	46041-46048	NN	denotes	carrier
T10267	46049-46056	NNS	denotes	females
T10270	46056-46057	.	denotes	?
T10271	46057-46291	sentence	denotes	One possibility is that, like some other X-linked genes, silencing of the Atrx gene on Xp is incomplete, such that there is always a low-level, leaky output of Atrx from a normally inactivated Xp chromosome in extraembryonic tissues.
T10272	46058-46061	CD	denotes	One
T10273	46062-46073	NN	denotes	possibility
T10274	46074-46076	VBZ	denotes	is
T10275	46077-46081	IN	denotes	that
T10277	46081-46083	,	denotes	,
T10278	46083-46087	IN	denotes	like
T10279	46088-46092	DT	denotes	some
T10281	46093-46098	JJ	denotes	other
T10282	46099-46100	NN	denotes	X
T10284	46100-46101	HYPH	denotes	-
T10283	46101-46107	JJ	denotes	linked
T10280	46108-46113	NNS	denotes	genes
T10285	46113-46115	,	denotes	,
T10286	46115-46124	NN	denotes	silencing
T10287	46125-46127	IN	denotes	of
T10288	46128-46131	DT	denotes	the
T10290	46132-46136	NN	denotes	Atrx
T10289	46137-46141	NN	denotes	gene
T10291	46142-46144	IN	denotes	on
T10292	46145-46147	NN	denotes	Xp
T10276	46148-46150	VBZ	denotes	is
T10293	46151-46161	JJ	denotes	incomplete
T10294	46161-46163	,	denotes	,
T10295	46163-46167	JJ	denotes	such
T10297	46168-46172	IN	denotes	that
T10298	46173-46178	EX	denotes	there
T10296	46179-46181	VBZ	denotes	is
T10299	46182-46188	RB	denotes	always
T10300	46189-46190	DT	denotes	a
T10302	46191-46194	JJ	denotes	low
T10304	46194-46195	HYPH	denotes	-
T10303	46195-46200	NN	denotes	level
T10305	46200-46202	,	denotes	,
T10306	46202-46207	JJ	denotes	leaky
T10301	46208-46214	NN	denotes	output
T10307	46215-46217	IN	denotes	of
T10308	46218-46222	NN	denotes	Atrx
T10309	46223-46227	IN	denotes	from
T10310	46228-46229	DT	denotes	a
T10312	46230-46238	RB	denotes	normally
T10313	46239-46250	VBN	denotes	inactivated
T10314	46251-46253	NN	denotes	Xp
T10311	46254-46264	NN	denotes	chromosome
T10315	46265-46267	IN	denotes	in
T10316	46268-46282	JJ	denotes	extraembryonic
T10317	46283-46290	NNS	denotes	tissues
T10318	46290-46291	.	denotes	.
T10319	46291-46569	sentence	denotes	However, it was recently demonstrated that the paternal Atrx (called Xnp) allele is completely silenced in a normal mouse trophoblast stem cell line [32], suggesting that Atrx does not normally escape imprinted X-inactivation in the extraembryonic tissues of wild-type females.
T10320	46292-46299	RB	denotes	However
T10322	46299-46301	,	denotes	,
T10323	46301-46303	PRP	denotes	it
T10324	46304-46307	VBD	denotes	was
T10325	46308-46316	RB	denotes	recently
T10321	46317-46329	VBN	denotes	demonstrated
T10326	46330-46334	IN	denotes	that
T10328	46335-46338	DT	denotes	the
T10330	46339-46347	JJ	denotes	paternal
T10331	46348-46352	NN	denotes	Atrx
T10332	46353-46354	-LRB-	denotes	(
T10333	46354-46360	VBN	denotes	called
T10334	46361-46364	NN	denotes	Xnp
T10335	46364-46365	-RRB-	denotes	)
T10329	46366-46372	NN	denotes	allele
T10336	46373-46375	VBZ	denotes	is
T10337	46376-46386	RB	denotes	completely
T10327	46387-46395	VBN	denotes	silenced
T10338	46396-46398	IN	denotes	in
T10339	46399-46400	DT	denotes	a
T10341	46401-46407	JJ	denotes	normal
T10342	46408-46413	NN	denotes	mouse
T10343	46414-46425	NN	denotes	trophoblast
T10344	46426-46430	NN	denotes	stem
T10345	46431-46435	NN	denotes	cell
T10340	46436-46440	NN	denotes	line
T10346	46441-46442	-LRB-	denotes	[
T10347	46442-46444	CD	denotes	32
T10348	46444-46445	-RRB-	denotes	]
T10349	46445-46447	,	denotes	,
T10350	46447-46457	VBG	denotes	suggesting
T10351	46458-46462	IN	denotes	that
T10353	46463-46467	NN	denotes	Atrx
T10354	46468-46472	VBZ	denotes	does
T10355	46473-46476	RB	denotes	not
T10356	46477-46485	RB	denotes	normally
T10352	46486-46492	VB	denotes	escape
T10357	46493-46502	VBN	denotes	imprinted
T10359	46503-46504	NN	denotes	X
T10360	46504-46505	HYPH	denotes	-
T10358	46505-46517	NN	denotes	inactivation
T10361	46518-46520	IN	denotes	in
T10362	46521-46524	DT	denotes	the
T10364	46525-46539	JJ	denotes	extraembryonic
T10363	46540-46547	NNS	denotes	tissues
T10365	46548-46550	IN	denotes	of
T10366	46551-46555	JJ	denotes	wild
T10368	46555-46556	HYPH	denotes	-
T10367	46556-46560	NN	denotes	type
T10369	46561-46568	NNS	denotes	females
T10370	46568-46569	.	denotes	.
T10371	46569-46693	sentence	denotes	Thus, the expression of the Xp-linked Atrx WT allele that we observed is unique to female carriers of the Atrx null allele.
T10372	46570-46574	RB	denotes	Thus
T10374	46574-46576	,	denotes	,
T10375	46576-46579	DT	denotes	the
T10376	46580-46590	NN	denotes	expression
T10377	46591-46593	IN	denotes	of
T10378	46594-46597	DT	denotes	the
T10380	46598-46600	NN	denotes	Xp
T10382	46600-46601	HYPH	denotes	-
T10381	46601-46607	VBN	denotes	linked
T10383	46608-46615	NN	denotes	Atrx WT
T10379	46616-46622	NN	denotes	allele
T10384	46623-46627	WDT	denotes	that
T10386	46628-46630	PRP	denotes	we
T10385	46631-46639	VBD	denotes	observed
T10373	46640-46642	VBZ	denotes	is
T10387	46643-46649	JJ	denotes	unique
T10388	46650-46652	IN	denotes	to
T10389	46653-46659	JJ	denotes	female
T10390	46660-46668	NNS	denotes	carriers
T10391	46669-46671	IN	denotes	of
T10392	46672-46675	DT	denotes	the
T10394	46676-46685	JJ	denotes	Atrx null
T10393	46686-46692	NN	denotes	allele
T10395	46692-46693	.	denotes	.
T10396	46693-47014	sentence	denotes	Perhaps a more likely explanation for this phenomenon stems from experimental observations suggesting that imprinted X-inactivation is not imposed on all precursors of the mouse extraembryonic tissues: A subpopulation of cells may escape this process and make a random “choice” of which X chromosome will be inactivated.
T10397	46694-46701	RB	denotes	Perhaps
T10399	46702-46703	DT	denotes	a
T10401	46704-46708	RBR	denotes	more
T10402	46709-46715	JJ	denotes	likely
T10400	46716-46727	NN	denotes	explanation
T10403	46728-46731	IN	denotes	for
T10404	46732-46736	DT	denotes	this
T10405	46737-46747	NN	denotes	phenomenon
T10398	46748-46753	VBZ	denotes	stems
T10406	46754-46758	IN	denotes	from
T10407	46759-46771	JJ	denotes	experimental
T10408	46772-46784	NNS	denotes	observations
T10409	46785-46795	VBG	denotes	suggesting
T10410	46796-46800	IN	denotes	that
T10412	46801-46810	VBN	denotes	imprinted
T10414	46811-46812	NN	denotes	X
T10415	46812-46813	HYPH	denotes	-
T10413	46813-46825	NN	denotes	inactivation
T10417	46826-46828	VBZ	denotes	is
T10418	46829-46832	RB	denotes	not
T10416	46833-46840	VBN	denotes	imposed
T10419	46841-46843	IN	denotes	on
T10420	46844-46847	DT	denotes	all
T10421	46848-46858	NNS	denotes	precursors
T10422	46859-46861	IN	denotes	of
T10423	46862-46865	DT	denotes	the
T10425	46866-46871	NN	denotes	mouse
T10426	46872-46886	JJ	denotes	extraembryonic
T10424	46887-46894	NNS	denotes	tissues
T10427	46894-46896	:	denotes	:
T10428	46896-46897	DT	denotes	A
T10429	46898-46911	NN	denotes	subpopulation
T10430	46912-46914	IN	denotes	of
T10431	46915-46920	NNS	denotes	cells
T10432	46921-46924	MD	denotes	may
T10411	46925-46931	VB	denotes	escape
T10433	46932-46936	DT	denotes	this
T10434	46937-46944	NN	denotes	process
T10435	46945-46948	CC	denotes	and
T10436	46949-46953	VB	denotes	make
T10437	46954-46955	DT	denotes	a
T10439	46956-46962	JJ	denotes	random
T10440	46963-46964	``	denotes	“
T10438	46964-46970	NN	denotes	choice
T10441	46970-46971	''	denotes	”
T10442	46972-46974	IN	denotes	of
T10443	46975-46980	WDT	denotes	which
T10445	46981-46982	NN	denotes	X
T10444	46983-46993	NN	denotes	chromosome
T10447	46994-46998	MD	denotes	will
T10448	46999-47001	VB	denotes	be
T10446	47002-47013	VBN	denotes	inactivated
T10449	47013-47014	.	denotes	.
T10450	47014-47142	sentence	denotes	On average, 50% of the cells in this randomly inactivating subpopulation would be expected to maintain an active Xp chromosome.
T10451	47015-47017	IN	denotes	On
T10453	47018-47025	JJ	denotes	average
T10454	47025-47027	,	denotes	,
T10455	47027-47029	CD	denotes	50
T10456	47029-47030	NN	denotes	%
T10457	47031-47033	IN	denotes	of
T10458	47034-47037	DT	denotes	the
T10459	47038-47043	NNS	denotes	cells
T10460	47044-47046	IN	denotes	in
T10461	47047-47051	DT	denotes	this
T10463	47052-47060	RB	denotes	randomly
T10464	47061-47073	VBG	denotes	inactivating
T10462	47074-47087	NN	denotes	subpopulation
T10465	47088-47093	MD	denotes	would
T10466	47094-47096	VB	denotes	be
T10452	47097-47105	VBN	denotes	expected
T10467	47106-47108	TO	denotes	to
T10468	47109-47117	VB	denotes	maintain
T10469	47118-47120	DT	denotes	an
T10471	47121-47127	JJ	denotes	active
T10472	47128-47130	NN	denotes	Xp
T10470	47131-47141	NN	denotes	chromosome
T10473	47141-47142	.	denotes	.
T10474	47142-47358	sentence	denotes	In support of this hypothesis, it has been demonstrated that expression of paternally transmitted X-linked lacZ [33,34] and GFP [35] transgenes failed to be silenced in a small subpopulation of extraembryonic cells.
T10475	47143-47145	IN	denotes	In
T10477	47146-47153	NN	denotes	support
T10478	47154-47156	IN	denotes	of
T10479	47157-47161	DT	denotes	this
T10480	47162-47172	NN	denotes	hypothesis
T10481	47172-47174	,	denotes	,
T10482	47174-47176	PRP	denotes	it
T10483	47177-47180	VBZ	denotes	has
T10484	47181-47185	VBN	denotes	been
T10476	47186-47198	VBN	denotes	demonstrated
T10485	47199-47203	IN	denotes	that
T10487	47204-47214	NN	denotes	expression
T10488	47215-47217	IN	denotes	of
T10489	47218-47228	RB	denotes	paternally
T10490	47229-47240	VBN	denotes	transmitted
T10492	47241-47242	NN	denotes	X
T10494	47242-47243	HYPH	denotes	-
T10493	47243-47249	VBN	denotes	linked
T10491	47250-47254	NN	denotes	lacZ
T10496	47255-47256	-LRB-	denotes	[
T10498	47256-47258	CD	denotes	33
T10499	47258-47259	,	denotes	,
T10497	47259-47261	CD	denotes	34
T10500	47261-47262	-RRB-	denotes	]
T10501	47263-47266	CC	denotes	and
T10502	47267-47270	NN	denotes	GFP
T10503	47271-47272	-LRB-	denotes	[
T10504	47272-47274	CD	denotes	35
T10505	47274-47275	-RRB-	denotes	]
T10495	47276-47286	NNS	denotes	transgenes
T10486	47287-47293	VBD	denotes	failed
T10506	47294-47296	TO	denotes	to
T10508	47297-47299	VB	denotes	be
T10507	47300-47308	VBN	denotes	silenced
T10509	47309-47311	IN	denotes	in
T10510	47312-47313	DT	denotes	a
T10512	47314-47319	JJ	denotes	small
T10511	47320-47333	NN	denotes	subpopulation
T10513	47334-47336	IN	denotes	of
T10514	47337-47351	JJ	denotes	extraembryonic
T10515	47352-47357	NNS	denotes	cells
T10516	47357-47358	.	denotes	.
T10517	47358-47552	sentence	denotes	Further, it has been shown that in a subpopulation of extraembryonic cells, it is the Xm rather than the Xp that undergoes late replication, a molecular correlate of the inactive state [18,36].
T10518	47359-47366	RB	denotes	Further
T10520	47366-47368	,	denotes	,
T10521	47368-47370	PRP	denotes	it
T10522	47371-47374	VBZ	denotes	has
T10523	47375-47379	VBN	denotes	been
T10519	47380-47385	VBN	denotes	shown
T10524	47386-47390	IN	denotes	that
T10526	47391-47393	IN	denotes	in
T10527	47394-47395	DT	denotes	a
T10528	47396-47409	NN	denotes	subpopulation
T10529	47410-47412	IN	denotes	of
T10530	47413-47427	JJ	denotes	extraembryonic
T10531	47428-47433	NNS	denotes	cells
T10532	47433-47435	,	denotes	,
T10533	47435-47437	PRP	denotes	it
T10525	47438-47440	VBZ	denotes	is
T10534	47441-47444	DT	denotes	the
T10535	47445-47447	NN	denotes	Xm
T10536	47448-47454	JJ	denotes	rather
T10537	47455-47459	IN	denotes	than
T10538	47460-47463	DT	denotes	the
T10539	47464-47466	NN	denotes	Xp
T10540	47467-47471	WDT	denotes	that
T10541	47472-47481	VBZ	denotes	undergoes
T10542	47482-47486	JJ	denotes	late
T10543	47487-47498	NN	denotes	replication
T10544	47498-47500	,	denotes	,
T10545	47500-47501	DT	denotes	a
T10547	47502-47511	JJ	denotes	molecular
T10546	47512-47521	NN	denotes	correlate
T10548	47522-47524	IN	denotes	of
T10549	47525-47528	DT	denotes	the
T10551	47529-47537	JJ	denotes	inactive
T10550	47538-47543	NN	denotes	state
T10552	47544-47545	-LRB-	denotes	[
T10554	47545-47547	CD	denotes	18
T10555	47547-47548	,	denotes	,
T10553	47548-47550	CD	denotes	36
T10556	47550-47551	-RRB-	denotes	]
T10557	47551-47552	.	denotes	.
T10558	47552-47840	sentence	denotes	Although initially small and quickly diluted in normal embryos, the cellular subpopulation that inactivates the Xm chromosome could rapidly expand to replace the normally imprinted cells in extraembryonic lineages if the normal silencing of Xp compromises cell growth or differentiation.
T10559	47553-47561	IN	denotes	Although
T10561	47562-47571	RB	denotes	initially
T10560	47572-47577	JJ	denotes	small
T10563	47578-47581	CC	denotes	and
T10564	47582-47589	RB	denotes	quickly
T10565	47590-47597	VBN	denotes	diluted
T10566	47598-47600	IN	denotes	in
T10567	47601-47607	JJ	denotes	normal
T10568	47608-47615	NNS	denotes	embryos
T10569	47615-47617	,	denotes	,
T10570	47617-47620	DT	denotes	the
T10572	47621-47629	JJ	denotes	cellular
T10571	47630-47643	NN	denotes	subpopulation
T10573	47644-47648	WDT	denotes	that
T10574	47649-47660	VBZ	denotes	inactivates
T10575	47661-47664	DT	denotes	the
T10577	47665-47667	NN	denotes	Xm
T10576	47668-47678	NN	denotes	chromosome
T10578	47679-47684	MD	denotes	could
T10579	47685-47692	RB	denotes	rapidly
T10562	47693-47699	VB	denotes	expand
T10580	47700-47702	TO	denotes	to
T10581	47703-47710	VB	denotes	replace
T10582	47711-47714	DT	denotes	the
T10584	47715-47723	RB	denotes	normally
T10585	47724-47733	VBN	denotes	imprinted
T10583	47734-47739	NNS	denotes	cells
T10586	47740-47742	IN	denotes	in
T10587	47743-47757	JJ	denotes	extraembryonic
T10588	47758-47766	NNS	denotes	lineages
T10589	47767-47769	IN	denotes	if
T10591	47770-47773	DT	denotes	the
T10593	47774-47780	JJ	denotes	normal
T10592	47781-47790	NN	denotes	silencing
T10594	47791-47793	IN	denotes	of
T10595	47794-47796	NN	denotes	Xp
T10590	47797-47808	VBZ	denotes	compromises
T10596	47809-47813	NN	denotes	cell
T10597	47814-47820	NN	denotes	growth
T10598	47821-47823	CC	denotes	or
T10599	47824-47839	NN	denotes	differentiation
T10600	47839-47840	.	denotes	.
T10601	47840-48194	sentence	denotes	Interestingly, it has been suggested that the size of the population that initially escapes imprinting may range widely (from 0% to 30%), even between genetically identical embryos [37], and this may account for the variable phenotype observed among females bearing Xm-linked mutant alleles of genes essential for normal extraembryonic development [38].
T10602	47841-47854	RB	denotes	Interestingly
T10604	47854-47856	,	denotes	,
T10605	47856-47858	PRP	denotes	it
T10606	47859-47862	VBZ	denotes	has
T10607	47863-47867	VBN	denotes	been
T10603	47868-47877	VBN	denotes	suggested
T10608	47878-47882	IN	denotes	that
T10610	47883-47886	DT	denotes	the
T10611	47887-47891	NN	denotes	size
T10612	47892-47894	IN	denotes	of
T10613	47895-47898	DT	denotes	the
T10614	47899-47909	NN	denotes	population
T10615	47910-47914	WDT	denotes	that
T10617	47915-47924	RB	denotes	initially
T10616	47925-47932	VBZ	denotes	escapes
T10618	47933-47943	NN	denotes	imprinting
T10619	47944-47947	MD	denotes	may
T10609	47948-47953	VB	denotes	range
T10620	47954-47960	RB	denotes	widely
T10621	47961-47962	-LRB-	denotes	(
T10622	47962-47966	IN	denotes	from
T10623	47967-47968	CD	denotes	0
T10624	47968-47969	NN	denotes	%
T10625	47970-47972	IN	denotes	to
T10626	47973-47975	CD	denotes	30
T10627	47975-47976	NN	denotes	%
T10628	47976-47977	-RRB-	denotes	)
T10629	47977-47979	,	denotes	,
T10630	47979-47983	RB	denotes	even
T10631	47984-47991	IN	denotes	between
T10632	47992-48003	RB	denotes	genetically
T10633	48004-48013	JJ	denotes	identical
T10634	48014-48021	NNS	denotes	embryos
T10635	48022-48023	-LRB-	denotes	[
T10636	48023-48025	CD	denotes	37
T10637	48025-48026	-RRB-	denotes	]
T10638	48026-48028	,	denotes	,
T10639	48028-48031	CC	denotes	and
T10640	48032-48036	DT	denotes	this
T10642	48037-48040	MD	denotes	may
T10641	48041-48048	VB	denotes	account
T10643	48049-48052	IN	denotes	for
T10644	48053-48056	DT	denotes	the
T10646	48057-48065	JJ	denotes	variable
T10645	48066-48075	NN	denotes	phenotype
T10647	48076-48084	VBN	denotes	observed
T10648	48085-48090	IN	denotes	among
T10649	48091-48098	NNS	denotes	females
T10650	48099-48106	VBG	denotes	bearing
T10651	48107-48109	NN	denotes	Xm
T10653	48109-48110	HYPH	denotes	-
T10652	48110-48116	VBN	denotes	linked
T10655	48117-48123	NN	denotes	mutant
T10654	48124-48131	NNS	denotes	alleles
T10656	48132-48134	IN	denotes	of
T10657	48135-48140	NNS	denotes	genes
T10658	48141-48150	JJ	denotes	essential
T10659	48151-48154	IN	denotes	for
T10660	48155-48161	JJ	denotes	normal
T10662	48162-48176	JJ	denotes	extraembryonic
T10661	48177-48188	NN	denotes	development
T10663	48189-48190	-LRB-	denotes	[
T10664	48190-48192	CD	denotes	38
T10665	48192-48193	-RRB-	denotes	]
T10666	48193-48194	.	denotes	.
T10667	48194-48347	sentence	denotes	Put simply, carrier females bearing a small initial population of escaping cells would be more severely affected than those bearing a larger population.
T10668	48195-48198	VBN	denotes	Put
T10670	48199-48205	RB	denotes	simply
T10671	48205-48207	,	denotes	,
T10672	48207-48214	NN	denotes	carrier
T10673	48215-48222	NNS	denotes	females
T10674	48223-48230	VBG	denotes	bearing
T10675	48231-48232	DT	denotes	a
T10677	48233-48238	JJ	denotes	small
T10678	48239-48246	JJ	denotes	initial
T10676	48247-48257	NN	denotes	population
T10679	48258-48260	IN	denotes	of
T10680	48261-48269	VBG	denotes	escaping
T10681	48270-48275	NNS	denotes	cells
T10682	48276-48281	MD	denotes	would
T10669	48282-48284	VB	denotes	be
T10683	48285-48289	RBR	denotes	more
T10684	48290-48298	RB	denotes	severely
T10685	48299-48307	VBN	denotes	affected
T10686	48308-48312	IN	denotes	than
T10687	48313-48318	DT	denotes	those
T10688	48319-48326	VBG	denotes	bearing
T10689	48327-48328	DT	denotes	a
T10691	48329-48335	JJR	denotes	larger
T10690	48336-48346	NN	denotes	population
T10692	48346-48347	.	denotes	.
T10693	48347-48534	sentence	denotes	This could explain why we have observed significant phenotypic variation among Atrx carrier females, with some carriers dying in utero by 9.5 dpc (Table 1) and others developing to term.
T10694	48348-48352	DT	denotes	This
T10696	48353-48358	MD	denotes	could
T10695	48359-48366	VB	denotes	explain
T10697	48367-48370	WRB	denotes	why
T10699	48371-48373	PRP	denotes	we
T10700	48374-48378	VBP	denotes	have
T10698	48379-48387	VBN	denotes	observed
T10701	48388-48399	JJ	denotes	significant
T10703	48400-48410	JJ	denotes	phenotypic
T10702	48411-48420	NN	denotes	variation
T10704	48421-48426	IN	denotes	among
T10705	48427-48431	NN	denotes	Atrx
T10706	48432-48439	NN	denotes	carrier
T10707	48440-48447	NNS	denotes	females
T10708	48447-48449	,	denotes	,
T10709	48449-48453	IN	denotes	with
T10710	48454-48458	DT	denotes	some
T10711	48459-48467	NNS	denotes	carriers
T10712	48468-48473	VBG	denotes	dying
T10713	48474-48476	FW	denotes	in
T10714	48477-48482	FW	denotes	utero
T10715	48483-48485	IN	denotes	by
T10716	48486-48489	CD	denotes	9.5
T10717	48490-48493	NNS	denotes	dpc
T10718	48494-48495	-LRB-	denotes	(
T10719	48495-48500	NN	denotes	Table
T10720	48501-48502	CD	denotes	1
T10721	48502-48503	-RRB-	denotes	)
T10722	48504-48507	CC	denotes	and
T10723	48508-48514	NNS	denotes	others
T10724	48515-48525	VBG	denotes	developing
T10725	48526-48528	IN	denotes	to
T10726	48529-48533	NN	denotes	term
T10727	48533-48534	.	denotes	.
T10728	48534-48703	sentence	denotes	Another possible mechanism is that inactivation of the paternal X proceeds normally in all cells, but subsequently the Atrx gene within individual cells is reactivated.
T10729	48535-48542	DT	denotes	Another
T10731	48543-48551	JJ	denotes	possible
T10730	48552-48561	NN	denotes	mechanism
T10732	48562-48564	VBZ	denotes	is
T10733	48565-48569	IN	denotes	that
T10735	48570-48582	NN	denotes	inactivation
T10736	48583-48585	IN	denotes	of
T10737	48586-48589	DT	denotes	the
T10739	48590-48598	JJ	denotes	paternal
T10738	48599-48600	NN	denotes	X
T10734	48601-48609	VBZ	denotes	proceeds
T10740	48610-48618	RB	denotes	normally
T10741	48619-48621	IN	denotes	in
T10742	48622-48625	DT	denotes	all
T10743	48626-48631	NNS	denotes	cells
T10744	48631-48633	,	denotes	,
T10745	48633-48636	CC	denotes	but
T10746	48637-48649	RB	denotes	subsequently
T10748	48650-48653	DT	denotes	the
T10750	48654-48658	NN	denotes	Atrx
T10749	48659-48663	NN	denotes	gene
T10751	48664-48670	IN	denotes	within
T10752	48671-48681	JJ	denotes	individual
T10753	48682-48687	NNS	denotes	cells
T10754	48688-48690	VBZ	denotes	is
T10747	48691-48702	VBN	denotes	reactivated
T10755	48702-48703	.	denotes	.
T10756	48703-48816	sentence	denotes	Alternatively, in the absence of Atrx, the paternal allele may partially escape the normal process of silencing.
T10757	48704-48717	RB	denotes	Alternatively
T10759	48717-48719	,	denotes	,
T10760	48719-48721	IN	denotes	in
T10761	48722-48725	DT	denotes	the
T10762	48726-48733	NN	denotes	absence
T10763	48734-48736	IN	denotes	of
T10764	48737-48741	NN	denotes	Atrx
T10765	48741-48743	,	denotes	,
T10766	48743-48746	DT	denotes	the
T10768	48747-48755	JJ	denotes	paternal
T10767	48756-48762	NN	denotes	allele
T10769	48763-48766	MD	denotes	may
T10770	48767-48776	RB	denotes	partially
T10758	48777-48783	VB	denotes	escape
T10771	48784-48787	DT	denotes	the
T10773	48788-48794	JJ	denotes	normal
T10772	48795-48802	NN	denotes	process
T10774	48803-48805	IN	denotes	of
T10775	48806-48815	NN	denotes	silencing
T10776	48815-48816	.	denotes	.
T10777	48816-49070	sentence	denotes	In both of these cases, other genes on the paternal X chromosome must be inactivated and remain so, since blocking inactivation of the entire Xp chromosome causes embryonic lethality due to biallelic expression of X-linked genes in the trophoblast [39].
T10778	48817-48819	IN	denotes	In
T10780	48820-48824	DT	denotes	both
T10781	48825-48827	IN	denotes	of
T10782	48828-48833	DT	denotes	these
T10783	48834-48839	NNS	denotes	cases
T10784	48839-48841	,	denotes	,
T10785	48841-48846	JJ	denotes	other
T10786	48847-48852	NNS	denotes	genes
T10787	48853-48855	IN	denotes	on
T10788	48856-48859	DT	denotes	the
T10790	48860-48868	JJ	denotes	paternal
T10791	48869-48870	NN	denotes	X
T10789	48871-48881	NN	denotes	chromosome
T10792	48882-48886	MD	denotes	must
T10793	48887-48889	VB	denotes	be
T10779	48890-48901	VBN	denotes	inactivated
T10794	48902-48905	CC	denotes	and
T10795	48906-48912	VBP	denotes	remain
T10796	48913-48915	RB	denotes	so
T10797	48915-48917	,	denotes	,
T10798	48917-48922	IN	denotes	since
T10800	48923-48931	VBG	denotes	blocking
T10801	48932-48944	NN	denotes	inactivation
T10802	48945-48947	IN	denotes	of
T10803	48948-48951	DT	denotes	the
T10805	48952-48958	JJ	denotes	entire
T10806	48959-48961	NN	denotes	Xp
T10804	48962-48972	NN	denotes	chromosome
T10799	48973-48979	VBZ	denotes	causes
T10807	48980-48989	JJ	denotes	embryonic
T10808	48990-48999	NN	denotes	lethality
T10809	49000-49003	IN	denotes	due
T10810	49004-49006	IN	denotes	to
T10811	49007-49016	JJ	denotes	biallelic
T10812	49017-49027	NN	denotes	expression
T10813	49028-49030	IN	denotes	of
T10814	49031-49032	NN	denotes	X
T10816	49032-49033	HYPH	denotes	-
T10815	49033-49039	VBN	denotes	linked
T10817	49040-49045	NNS	denotes	genes
T10818	49046-49048	IN	denotes	in
T10819	49049-49052	DT	denotes	the
T10820	49053-49064	NN	denotes	trophoblast
T10821	49065-49066	-LRB-	denotes	[
T10822	49066-49068	CD	denotes	39
T10823	49068-49069	-RRB-	denotes	]
T10824	49069-49070	.	denotes	.
T10925	49072-49079	NN	denotes	Summary
T10926	49079-49196	sentence	denotes	ATR-X syndrome is the first human genetic disease known to be caused by mutations in a chromatin remodelling factor.
T10927	49080-49083	NN	denotes	ATR
T10929	49083-49084	HYPH	denotes	-
T10928	49084-49085	NN	denotes	X
T10930	49086-49094	NN	denotes	syndrome
T10931	49095-49097	VBZ	denotes	is
T10932	49098-49101	DT	denotes	the
T10934	49102-49107	JJ	denotes	first
T10935	49108-49113	JJ	denotes	human
T10936	49114-49121	JJ	denotes	genetic
T10933	49122-49129	NN	denotes	disease
T10937	49130-49135	VBN	denotes	known
T10938	49136-49138	TO	denotes	to
T10940	49139-49141	VB	denotes	be
T10939	49142-49148	VBN	denotes	caused
T10941	49149-49151	IN	denotes	by
T10942	49152-49161	NNS	denotes	mutations
T10943	49162-49164	IN	denotes	in
T10944	49165-49166	DT	denotes	a
T10946	49167-49176	NN	denotes	chromatin
T10947	49177-49188	NN	denotes	remodelling
T10945	49189-49195	NN	denotes	factor
T10948	49195-49196	.	denotes	.
T10949	49196-49299	sentence	denotes	At present we do not know how ATRX influences gene expression or what effect it has on cell behaviour.
T10950	49197-49199	IN	denotes	At
T10952	49200-49207	JJ	denotes	present
T10953	49208-49210	PRP	denotes	we
T10954	49211-49213	VBP	denotes	do
T10955	49214-49217	RB	denotes	not
T10951	49218-49222	VB	denotes	know
T10956	49223-49226	WRB	denotes	how
T10958	49227-49231	NN	denotes	ATRX
T10957	49232-49242	VBZ	denotes	influences
T10959	49243-49247	NN	denotes	gene
T10960	49248-49258	NN	denotes	expression
T10961	49259-49261	CC	denotes	or
T10962	49262-49266	WDT	denotes	what
T10963	49267-49273	NN	denotes	effect
T10965	49274-49276	PRP	denotes	it
T10964	49277-49280	VBZ	denotes	has
T10966	49281-49283	IN	denotes	on
T10967	49284-49288	NN	denotes	cell
T10968	49289-49298	NN	denotes	behaviour
T10969	49298-49299	.	denotes	.
T10970	49299-49475	sentence	denotes	Nevertheless, we have previously noted that none of the natural mutations causing ATR-X syndrome are nulls, which suggests that it plays a critical role in normal development.
T10971	49300-49312	RB	denotes	Nevertheless
T10973	49312-49314	,	denotes	,
T10974	49314-49316	PRP	denotes	we
T10975	49317-49321	VBP	denotes	have
T10976	49322-49332	RB	denotes	previously
T10972	49333-49338	VBN	denotes	noted
T10977	49339-49343	IN	denotes	that
T10979	49344-49348	NN	denotes	none
T10980	49349-49351	IN	denotes	of
T10981	49352-49355	DT	denotes	the
T10983	49356-49363	JJ	denotes	natural
T10982	49364-49373	NNS	denotes	mutations
T10984	49374-49381	VBG	denotes	causing
T10985	49382-49385	NN	denotes	ATR
T10987	49385-49386	HYPH	denotes	-
T10986	49386-49387	NN	denotes	X
T10988	49388-49396	NN	denotes	syndrome
T10978	49397-49400	VBP	denotes	are
T10989	49401-49406	NNS	denotes	nulls
T10990	49406-49408	,	denotes	,
T10991	49408-49413	WDT	denotes	which
T10992	49414-49422	VBZ	denotes	suggests
T10993	49423-49427	IN	denotes	that
T10995	49428-49430	PRP	denotes	it
T10994	49431-49436	VBZ	denotes	plays
T10996	49437-49438	DT	denotes	a
T10998	49439-49447	JJ	denotes	critical
T10997	49448-49452	NN	denotes	role
T10999	49453-49455	IN	denotes	in
T11000	49456-49462	JJ	denotes	normal
T11001	49463-49474	NN	denotes	development
T11002	49474-49475	.	denotes	.
T11003	49475-49677	sentence	denotes	Results of conditional inactivation of Atrx in the developing mouse forebrain, based on the Atrx flox allele described here, shows that Atrx exerts a major effect on terminally differentiating neurons.
T11004	49476-49483	NNS	denotes	Results
T11006	49484-49486	IN	denotes	of
T11007	49487-49498	JJ	denotes	conditional
T11008	49499-49511	NN	denotes	inactivation
T11009	49512-49514	IN	denotes	of
T11010	49515-49519	NN	denotes	Atrx
T11011	49520-49522	IN	denotes	in
T11012	49523-49526	DT	denotes	the
T11014	49527-49537	VBG	denotes	developing
T11015	49538-49543	NN	denotes	mouse
T11013	49544-49553	NN	denotes	forebrain
T11016	49553-49555	,	denotes	,
T11017	49555-49560	VBN	denotes	based
T11018	49561-49563	IN	denotes	on
T11019	49564-49567	DT	denotes	the
T11021	49568-49577	NN	denotes	Atrx flox
T11020	49578-49584	NN	denotes	allele
T11022	49585-49594	VBN	denotes	described
T11023	49595-49599	RB	denotes	here
T11024	49599-49601	,	denotes	,
T11005	49601-49606	VBZ	denotes	shows
T11025	49607-49611	IN	denotes	that
T11027	49612-49616	NN	denotes	Atrx
T11026	49617-49623	VBZ	denotes	exerts
T11028	49624-49625	DT	denotes	a
T11030	49626-49631	JJ	denotes	major
T11029	49632-49638	NN	denotes	effect
T11031	49639-49641	IN	denotes	on
T11032	49642-49652	RB	denotes	terminally
T11033	49653-49668	VBG	denotes	differentiating
T11034	49669-49676	NNS	denotes	neurons
T11035	49676-49677	.	denotes	.
T11036	49677-49740	sentence	denotes	Conditional inactivation of Atrx in other tissues is underway.
T11037	49678-49689	JJ	denotes	Conditional
T11038	49690-49702	NN	denotes	inactivation
T11040	49703-49705	IN	denotes	of
T11041	49706-49710	NN	denotes	Atrx
T11042	49711-49713	IN	denotes	in
T11043	49714-49719	JJ	denotes	other
T11044	49720-49727	NNS	denotes	tissues
T11039	49728-49730	VBZ	denotes	is
T11045	49731-49739	JJ	denotes	underway
T11046	49739-49740	.	denotes	.
T11047	49740-49929	sentence	denotes	Here we have shown that animal-wide disruption of the Atrx gene causes a severe embryonic-lethal phenotype, revealing an essential role for Atrx in the formation of the murine trophoblast.
T11048	49741-49745	RB	denotes	Here
T11050	49746-49748	PRP	denotes	we
T11051	49749-49753	VBP	denotes	have
T11049	49754-49759	VBN	denotes	shown
T11052	49760-49764	IN	denotes	that
T11054	49765-49771	NN	denotes	animal
T11056	49771-49772	HYPH	denotes	-
T11055	49772-49776	JJ	denotes	wide
T11057	49777-49787	NN	denotes	disruption
T11058	49788-49790	IN	denotes	of
T11059	49791-49794	DT	denotes	the
T11061	49795-49799	NN	denotes	Atrx
T11060	49800-49804	NN	denotes	gene
T11053	49805-49811	VBZ	denotes	causes
T11062	49812-49813	DT	denotes	a
T11064	49814-49820	JJ	denotes	severe
T11065	49821-49830	JJ	denotes	embryonic
T11067	49830-49831	HYPH	denotes	-
T11066	49831-49837	JJ	denotes	lethal
T11063	49838-49847	NN	denotes	phenotype
T11068	49847-49849	,	denotes	,
T11069	49849-49858	VBG	denotes	revealing
T11070	49859-49861	DT	denotes	an
T11072	49862-49871	JJ	denotes	essential
T11071	49872-49876	NN	denotes	role
T11073	49877-49880	IN	denotes	for
T11074	49881-49885	NN	denotes	Atrx
T11075	49886-49888	IN	denotes	in
T11076	49889-49892	DT	denotes	the
T11077	49893-49902	NN	denotes	formation
T11078	49903-49905	IN	denotes	of
T11079	49906-49909	DT	denotes	the
T11081	49910-49916	JJ	denotes	murine
T11080	49917-49928	NN	denotes	trophoblast
T11082	49928-49929	.	denotes	.
T11083	49929-50060	sentence	denotes	In addition, Atrx appears to escape imprinted X-chromosome inactivation in the extraembryonic tissues of some carrier female mice.
T11084	49930-49932	IN	denotes	In
T11086	49933-49941	NN	denotes	addition
T11087	49941-49943	,	denotes	,
T11088	49943-49947	NN	denotes	Atrx
T11085	49948-49955	VBZ	denotes	appears
T11089	49956-49958	TO	denotes	to
T11090	49959-49965	VB	denotes	escape
T11091	49966-49975	VBN	denotes	imprinted
T11093	49976-49977	NN	denotes	X
T11094	49977-49978	HYPH	denotes	-
T11092	49978-49988	NN	denotes	chromosome
T11095	49989-50001	NN	denotes	inactivation
T11096	50002-50004	IN	denotes	in
T11097	50005-50008	DT	denotes	the
T11099	50009-50023	JJ	denotes	extraembryonic
T11098	50024-50031	NNS	denotes	tissues
T11100	50032-50034	IN	denotes	of
T11101	50035-50039	DT	denotes	some
T11103	50040-50047	NN	denotes	carrier
T11104	50048-50054	JJ	denotes	female
T11102	50055-50059	NNS	denotes	mice
T11105	50059-50060	.	denotes	.
T11189	50085-50095	NN	denotes	Generation
T11190	50096-50098	IN	denotes	of
T11191	50099-50101	NN	denotes	ES
T11192	50102-50107	NNS	denotes	cells
T11193	50108-50115	VBG	denotes	bearing
T11194	50116-50119	DT	denotes	the
T11196	50120-50129	NN	denotes	Atrx flox
T11195	50130-50136	NN	denotes	allele
T11197	50136-50137	.	denotes	.
T11198	50137-50302	sentence	denotes	Briefly, the targeting vector (shown in Figure 2A) places a loxP site within intron 18 and a loxP-flanked MC1neopA selection cassette in intron 17 of the Atrx gene.
T11199	50138-50145	RB	denotes	Briefly
T11201	50145-50147	,	denotes	,
T11202	50147-50150	DT	denotes	the
T11204	50151-50160	NN	denotes	targeting
T11203	50161-50167	NN	denotes	vector
T11205	50168-50169	-LRB-	denotes	(
T11206	50169-50174	VBN	denotes	shown
T11207	50175-50177	IN	denotes	in
T11208	50178-50184	NN	denotes	Figure
T11209	50185-50187	NN	denotes	2A
T11210	50187-50188	-RRB-	denotes	)
T11200	50189-50195	VBZ	denotes	places
T11211	50196-50197	DT	denotes	a
T11213	50198-50202	NN	denotes	loxP
T11212	50203-50207	NN	denotes	site
T11214	50208-50214	IN	denotes	within
T11215	50215-50221	NN	denotes	intron
T11216	50222-50224	CD	denotes	18
T11217	50225-50228	CC	denotes	and
T11218	50229-50230	DT	denotes	a
T11220	50231-50235	NN	denotes	loxP
T11222	50235-50236	HYPH	denotes	-
T11221	50236-50243	VBN	denotes	flanked
T11223	50244-50252	NN	denotes	MC1neopA
T11224	50253-50262	NN	denotes	selection
T11219	50263-50271	NN	denotes	cassette
T11225	50272-50274	IN	denotes	in
T11226	50275-50281	NN	denotes	intron
T11227	50282-50284	CD	denotes	17
T11228	50285-50287	IN	denotes	of
T11229	50288-50291	DT	denotes	the
T11231	50292-50296	NN	denotes	Atrx
T11230	50297-50301	NN	denotes	gene
T11232	50301-50302	.	denotes	.
T11233	50302-50373	sentence	denotes	A detailed description of the targeting construct is provided in [20].
T11234	50303-50304	DT	denotes	A
T11236	50305-50313	VBN	denotes	detailed
T11235	50314-50325	NN	denotes	description
T11238	50326-50328	IN	denotes	of
T11239	50329-50332	DT	denotes	the
T11241	50333-50342	NN	denotes	targeting
T11240	50343-50352	NN	denotes	construct
T11242	50353-50355	VBZ	denotes	is
T11237	50356-50364	VBN	denotes	provided
T11243	50365-50367	IN	denotes	in
T11244	50368-50369	-LRB-	denotes	[
T11245	50369-50371	CD	denotes	20
T11246	50371-50372	-RRB-	denotes	]
T11247	50372-50373	.	denotes	.
T11248	50373-50513	sentence	denotes	Linearised plasmid (150 μg) was electroporated into 1 × 108 E14Tg2a ES cells, and colonies resistant to G418 and ganciclovir were isolated.
T11249	50374-50384	VBN	denotes	Linearised
T11250	50385-50392	NN	denotes	plasmid
T11252	50393-50394	-LRB-	denotes	(
T11254	50394-50397	CD	denotes	150
T11253	50398-50400	NNS	denotes	μg
T11255	50400-50401	-RRB-	denotes	)
T11256	50402-50405	VBD	denotes	was
T11251	50406-50420	VBN	denotes	electroporated
T11257	50421-50425	IN	denotes	into
T11258	50426-50427	CD	denotes	1
T11260	50428-50429	SYM	denotes	×
T11259	50430-50433	CD	denotes	108
T11262	50434-50441	NN	denotes	E14Tg2a
T11263	50442-50444	NN	denotes	ES
T11261	50445-50450	NNS	denotes	cells
T11264	50450-50452	,	denotes	,
T11265	50452-50455	CC	denotes	and
T11266	50456-50464	NNS	denotes	colonies
T11268	50465-50474	JJ	denotes	resistant
T11269	50475-50477	IN	denotes	to
T11270	50478-50482	NN	denotes	G418
T11271	50483-50486	CC	denotes	and
T11272	50487-50498	NN	denotes	ganciclovir
T11273	50499-50503	VBD	denotes	were
T11267	50504-50512	VBN	denotes	isolated
T11274	50512-50513	.	denotes	.
T11275	50513-50747	sentence	denotes	Homologous targeting events were identified by Southern blot of EcoRI-digested DNA and hybridisation with a 5′ probe (generated with primers PPS1.20 and PPS1.27) and a 3′ probe (a 0.9-kb HaeIII fragment) as shown in Figure 2A and 2B.
T11276	50514-50524	JJ	denotes	Homologous
T11278	50525-50534	NN	denotes	targeting
T11277	50535-50541	NNS	denotes	events
T11280	50542-50546	VBD	denotes	were
T11279	50547-50557	VBN	denotes	identified
T11281	50558-50560	IN	denotes	by
T11282	50561-50569	NNP	denotes	Southern
T11283	50570-50574	NN	denotes	blot
T11284	50575-50577	IN	denotes	of
T11285	50578-50583	NN	denotes	EcoRI
T11287	50583-50584	HYPH	denotes	-
T11286	50584-50592	VBN	denotes	digested
T11288	50593-50596	NN	denotes	DNA
T11289	50597-50600	CC	denotes	and
T11290	50601-50614	NN	denotes	hybridisation
T11291	50615-50619	IN	denotes	with
T11292	50620-50621	DT	denotes	a
T11294	50622-50623	CD	denotes	5
T11295	50623-50624	SYM	denotes	′
T11293	50625-50630	NN	denotes	probe
T11296	50631-50632	-LRB-	denotes	(
T11297	50632-50641	VBN	denotes	generated
T11298	50642-50646	IN	denotes	with
T11299	50647-50654	NNS	denotes	primers
T11300	50655-50662	NN	denotes	PPS1.20
T11301	50663-50666	CC	denotes	and
T11302	50667-50674	NN	denotes	PPS1.27
T11303	50674-50675	-RRB-	denotes	)
T11304	50676-50679	CC	denotes	and
T11305	50680-50681	DT	denotes	a
T11307	50682-50683	CD	denotes	3
T11308	50683-50684	SYM	denotes	′
T11306	50685-50690	NN	denotes	probe
T11309	50691-50692	-LRB-	denotes	(
T11310	50692-50693	DT	denotes	a
T11312	50694-50697	CD	denotes	0.9
T11314	50697-50698	HYPH	denotes	-
T11313	50698-50700	NN	denotes	kb
T11315	50701-50707	NN	denotes	HaeIII
T11311	50708-50716	NN	denotes	fragment
T11316	50716-50717	-RRB-	denotes	)
T11317	50718-50720	IN	denotes	as
T11318	50721-50726	VBN	denotes	shown
T11319	50727-50729	IN	denotes	in
T11320	50730-50736	NN	denotes	Figure
T11321	50737-50739	NN	denotes	2A
T11322	50740-50743	CC	denotes	and
T11323	50744-50746	NN	denotes	2B
T11324	50746-50747	.	denotes	.
T11325	50747-51066	sentence	denotes	DNA from correctly targeted clones was also digested with SacI and analysed by Southern blot and hybridisation with a probe from within intron 17 (a PCR product generated with primers PPS1.15 and Xnp46) to confirm that the loxP site within intron 18 had been included within the crossed-over region (Figure 2A and 2C).
T11326	50748-50751	NN	denotes	DNA
T11328	50752-50756	IN	denotes	from
T11329	50757-50766	RB	denotes	correctly
T11330	50767-50775	VBN	denotes	targeted
T11331	50776-50782	NNS	denotes	clones
T11332	50783-50786	VBD	denotes	was
T11333	50787-50791	RB	denotes	also
T11327	50792-50800	VBN	denotes	digested
T11334	50801-50805	IN	denotes	with
T11335	50806-50810	NN	denotes	SacI
T11336	50811-50814	CC	denotes	and
T11337	50815-50823	VBN	denotes	analysed
T11338	50824-50826	IN	denotes	by
T11339	50827-50835	NNP	denotes	Southern
T11340	50836-50840	NN	denotes	blot
T11341	50841-50844	CC	denotes	and
T11342	50845-50858	NN	denotes	hybridisation
T11343	50859-50863	IN	denotes	with
T11344	50864-50865	DT	denotes	a
T11345	50866-50871	NN	denotes	probe
T11346	50872-50876	IN	denotes	from
T11347	50877-50883	IN	denotes	within
T11348	50884-50890	NN	denotes	intron
T11349	50891-50893	CD	denotes	17
T11350	50894-50895	-LRB-	denotes	(
T11351	50895-50896	DT	denotes	a
T11353	50897-50900	NN	denotes	PCR
T11352	50901-50908	NN	denotes	product
T11354	50909-50918	VBN	denotes	generated
T11355	50919-50923	IN	denotes	with
T11356	50924-50931	NNS	denotes	primers
T11357	50932-50939	NN	denotes	PPS1.15
T11358	50940-50943	CC	denotes	and
T11359	50944-50949	NN	denotes	Xnp46
T11360	50949-50950	-RRB-	denotes	)
T11361	50951-50953	TO	denotes	to
T11362	50954-50961	VB	denotes	confirm
T11363	50962-50966	IN	denotes	that
T11365	50967-50970	DT	denotes	the
T11367	50971-50975	NN	denotes	loxP
T11366	50976-50980	NN	denotes	site
T11368	50981-50987	IN	denotes	within
T11369	50988-50994	NN	denotes	intron
T11370	50995-50997	CD	denotes	18
T11371	50998-51001	VBD	denotes	had
T11372	51002-51006	VBN	denotes	been
T11364	51007-51015	VBN	denotes	included
T11373	51016-51022	IN	denotes	within
T11374	51023-51026	DT	denotes	the
T11376	51027-51034	VBN	denotes	crossed
T11377	51034-51035	HYPH	denotes	-
T11378	51035-51039	RP	denotes	over
T11375	51040-51046	NN	denotes	region
T11379	51047-51048	-LRB-	denotes	(
T11381	51048-51054	NN	denotes	Figure
T11380	51055-51057	NN	denotes	2A
T11382	51058-51061	CC	denotes	and
T11383	51062-51064	NN	denotes	2C
T11384	51064-51065	-RRB-	denotes	)
T11385	51065-51066	.	denotes	.
T11386	51066-51110	sentence	denotes	Sequences of primers are shown in Table S1.
T11387	51067-51076	NNS	denotes	Sequences
T11389	51077-51079	IN	denotes	of
T11390	51080-51087	NNS	denotes	primers
T11391	51088-51091	VBP	denotes	are
T11388	51092-51097	VBN	denotes	shown
T11392	51098-51100	IN	denotes	in
T11393	51101-51106	NN	denotes	Table
T11394	51107-51109	NN	denotes	S1
T11395	51109-51110	.	denotes	.
T11569	51112-51115	NN	denotes	Cre
T11571	51115-51116	HYPH	denotes	-
T11570	51116-51129	NN	denotes	recombination
T11572	51130-51133	CC	denotes	and
T11573	51134-51150	NN	denotes	characterisation
T11574	51151-51153	IN	denotes	of
T11575	51154-51162	JJ	denotes	Atrxnull
T11577	51163-51165	NN	denotes	ES
T11576	51166-51171	NNS	denotes	cells
T11578	51172-51175	CC	denotes	and
T11579	51176-51184	JJ	denotes	embryoid
T11580	51185-51191	NNS	denotes	bodies
T11581	51191-51192	.	denotes	.
T11582	51192-51352	sentence	denotes	ES cell clones bearing the Atrx flox allele (1 × 107 cells) were transiently transfected with 50 μg of uncut Cre expression plasmid (pCAGGS-Cre-IRESpuro) [40].
T11583	51193-51195	NN	denotes	ES
T11584	51196-51200	NN	denotes	cell
T11585	51201-51207	NNS	denotes	clones
T11587	51208-51215	VBG	denotes	bearing
T11588	51216-51219	DT	denotes	the
T11590	51220-51229	NN	denotes	Atrx flox
T11589	51230-51236	NN	denotes	allele
T11591	51237-51238	-LRB-	denotes	(
T11593	51238-51239	CD	denotes	1
T11595	51240-51241	SYM	denotes	×
T11594	51242-51245	CD	denotes	107
T11592	51246-51251	NNS	denotes	cells
T11596	51251-51252	-RRB-	denotes	)
T11597	51253-51257	VBD	denotes	were
T11598	51258-51269	RB	denotes	transiently
T11586	51270-51281	VBN	denotes	transfected
T11599	51282-51286	IN	denotes	with
T11600	51287-51289	CD	denotes	50
T11601	51290-51292	NNS	denotes	μg
T11602	51293-51295	IN	denotes	of
T11603	51296-51301	JJ	denotes	uncut
T11605	51302-51305	NN	denotes	Cre
T11606	51306-51316	NN	denotes	expression
T11604	51317-51324	NN	denotes	plasmid
T11607	51325-51326	-LRB-	denotes	(
T11608	51326-51332	NN	denotes	pCAGGS
T11610	51332-51333	HYPH	denotes	-
T11611	51333-51336	NN	denotes	Cre
T11612	51336-51337	HYPH	denotes	-
T11609	51337-51345	NN	denotes	IRESpuro
T11613	51345-51346	-RRB-	denotes	)
T11614	51347-51348	-LRB-	denotes	[
T11615	51348-51350	CD	denotes	40
T11616	51350-51351	-RRB-	denotes	]
T11617	51351-51352	.	denotes	.
T11618	51352-51504	sentence	denotes	Following transfection, cells were plated at a range of clonal densities in complete medium without G418, and isolated subclones were picked after 7 d.
T11619	51353-51362	VBG	denotes	Following
T11621	51363-51375	NN	denotes	transfection
T11622	51375-51377	,	denotes	,
T11623	51377-51382	NNS	denotes	cells
T11624	51383-51387	VBD	denotes	were
T11620	51388-51394	VBN	denotes	plated
T11625	51395-51397	IN	denotes	at
T11626	51398-51399	DT	denotes	a
T11627	51400-51405	NN	denotes	range
T11628	51406-51408	IN	denotes	of
T11629	51409-51415	JJ	denotes	clonal
T11630	51416-51425	NNS	denotes	densities
T11631	51426-51428	IN	denotes	in
T11632	51429-51437	JJ	denotes	complete
T11633	51438-51444	NN	denotes	medium
T11634	51445-51452	IN	denotes	without
T11635	51453-51457	NN	denotes	G418
T11636	51457-51459	,	denotes	,
T11637	51459-51462	CC	denotes	and
T11638	51463-51471	VBN	denotes	isolated
T11639	51472-51481	NNS	denotes	subclones
T11641	51482-51486	VBD	denotes	were
T11640	51487-51493	VBN	denotes	picked
T11642	51494-51499	IN	denotes	after
T11643	51500-51501	CD	denotes	7
T11644	51502-51503	NNS	denotes	d
T11645	51503-51504	.	denotes	.
T11646	51504-51749	sentence	denotes	Subclones were expanded and analysed for the presence of a recombinant locus initially by PCR, to detect deletion of the MC1neopA cassette, and then by Southern blot and hybridisation with the intron 17 probe described above (Figure 2A and 2C).
T11647	51505-51514	NNS	denotes	Subclones
T11649	51515-51519	VBD	denotes	were
T11648	51520-51528	VBN	denotes	expanded
T11650	51529-51532	CC	denotes	and
T11651	51533-51541	VBN	denotes	analysed
T11652	51542-51545	IN	denotes	for
T11653	51546-51549	DT	denotes	the
T11654	51550-51558	NN	denotes	presence
T11655	51559-51561	IN	denotes	of
T11656	51562-51563	DT	denotes	a
T11658	51564-51575	JJ	denotes	recombinant
T11657	51576-51581	NN	denotes	locus
T11659	51582-51591	RB	denotes	initially
T11660	51592-51594	IN	denotes	by
T11661	51595-51598	NN	denotes	PCR
T11662	51598-51600	,	denotes	,
T11663	51600-51602	TO	denotes	to
T11664	51603-51609	VB	denotes	detect
T11665	51610-51618	NN	denotes	deletion
T11666	51619-51621	IN	denotes	of
T11667	51622-51625	DT	denotes	the
T11669	51626-51634	NN	denotes	MC1neopA
T11668	51635-51643	NN	denotes	cassette
T11670	51643-51645	,	denotes	,
T11671	51645-51648	CC	denotes	and
T11672	51649-51653	RB	denotes	then
T11673	51654-51656	IN	denotes	by
T11674	51657-51665	NNP	denotes	Southern
T11675	51666-51670	NN	denotes	blot
T11676	51671-51674	CC	denotes	and
T11677	51675-51688	NN	denotes	hybridisation
T11678	51689-51693	IN	denotes	with
T11679	51694-51697	DT	denotes	the
T11681	51698-51704	NN	denotes	intron
T11682	51705-51707	CD	denotes	17
T11680	51708-51713	NN	denotes	probe
T11683	51714-51723	VBN	denotes	described
T11684	51724-51729	RB	denotes	above
T11685	51730-51731	-LRB-	denotes	(
T11687	51731-51737	NN	denotes	Figure
T11686	51738-51740	NN	denotes	2A
T11688	51741-51744	CC	denotes	and
T11689	51745-51747	NN	denotes	2C
T11690	51747-51748	-RRB-	denotes	)
T11691	51748-51749	.	denotes	.
T11692	51749-51919	sentence	denotes	Northern blots were carried out according to standard techniques using 20 μg of total RNA isolated using TRI Reagent (Sigma-Aldrich, St. Louis, Missouri, United States).
T11693	51750-51758	NNP	denotes	Northern
T11694	51759-51764	NNS	denotes	blots
T11696	51765-51769	VBD	denotes	were
T11695	51770-51777	VBN	denotes	carried
T11697	51778-51781	RP	denotes	out
T11698	51782-51791	VBG	denotes	according
T11699	51792-51794	IN	denotes	to
T11700	51795-51803	JJ	denotes	standard
T11701	51804-51814	NNS	denotes	techniques
T11702	51815-51820	VBG	denotes	using
T11703	51821-51823	CD	denotes	20
T11704	51824-51826	NNS	denotes	μg
T11705	51827-51829	IN	denotes	of
T11706	51830-51835	JJ	denotes	total
T11707	51836-51839	NN	denotes	RNA
T11708	51840-51848	VBN	denotes	isolated
T11709	51849-51854	VBG	denotes	using
T11710	51855-51858	NN	denotes	TRI
T11711	51859-51866	NN	denotes	Reagent
T11712	51867-51868	-LRB-	denotes	(
T11714	51868-51873	NNP	denotes	Sigma
T11715	51873-51874	HYPH	denotes	-
T11713	51874-51881	NNP	denotes	Aldrich
T11716	51881-51883	,	denotes	,
T11717	51883-51886	NNP	denotes	St.
T11718	51887-51892	NNP	denotes	Louis
T11719	51892-51894	,	denotes	,
T11720	51894-51902	NNP	denotes	Missouri
T11721	51902-51904	,	denotes	,
T11722	51904-51910	NNP	denotes	United
T11723	51911-51917	NNP	denotes	States
T11724	51917-51918	-RRB-	denotes	)
T11725	51918-51919	.	denotes	.
T11726	51919-52047	sentence	denotes	The blot was hybridised with a probe from within exon 10 of the Atrx gene (generated with primers Mxnp4 and Mxnp28 [Table S1]).
T11727	51920-51923	DT	denotes	The
T11728	51924-51928	NN	denotes	blot
T11730	51929-51932	VBD	denotes	was
T11729	51933-51943	VBN	denotes	hybridised
T11731	51944-51948	IN	denotes	with
T11732	51949-51950	DT	denotes	a
T11733	51951-51956	NN	denotes	probe
T11734	51957-51961	IN	denotes	from
T11735	51962-51968	IN	denotes	within
T11736	51969-51973	NN	denotes	exon
T11737	51974-51976	CD	denotes	10
T11738	51977-51979	IN	denotes	of
T11739	51980-51983	DT	denotes	the
T11741	51984-51988	NN	denotes	Atrx
T11740	51989-51993	NN	denotes	gene
T11742	51994-51995	-LRB-	denotes	(
T11743	51995-52004	VBN	denotes	generated
T11744	52005-52009	IN	denotes	with
T11745	52010-52017	NNS	denotes	primers
T11746	52018-52023	NN	denotes	Mxnp4
T11747	52024-52027	CC	denotes	and
T11748	52028-52034	NN	denotes	Mxnp28
T11749	52035-52036	-LRB-	denotes	[
T11751	52036-52041	NN	denotes	Table
T11750	52042-52044	NN	denotes	S1
T11752	52044-52045	-RRB-	denotes	]
T11753	52045-52046	-RRB-	denotes	)
T11754	52046-52047	.	denotes	.
T11755	52047-52174	sentence	denotes	After it was stripped, the membrane was hybridised with a β-actin cDNA probe (Clontech, Palo Alto, California, United States).
T11756	52048-52053	IN	denotes	After
T11758	52054-52056	PRP	denotes	it
T11759	52057-52060	VBD	denotes	was
T11757	52061-52069	VBN	denotes	stripped
T11761	52069-52071	,	denotes	,
T11762	52071-52074	DT	denotes	the
T11763	52075-52083	NN	denotes	membrane
T11764	52084-52087	VBD	denotes	was
T11760	52088-52098	VBN	denotes	hybridised
T11765	52099-52103	IN	denotes	with
T11766	52104-52105	DT	denotes	a
T11768	52106-52107	NN	denotes	β
T11770	52107-52108	HYPH	denotes	-
T11769	52108-52113	NN	denotes	actin
T11771	52114-52118	NN	denotes	cDNA
T11767	52119-52124	NN	denotes	probe
T11772	52125-52126	-LRB-	denotes	(
T11773	52126-52134	NNP	denotes	Clontech
T11774	52134-52136	,	denotes	,
T11775	52136-52140	NNP	denotes	Palo
T11776	52141-52145	NNP	denotes	Alto
T11777	52145-52147	,	denotes	,
T11778	52147-52157	NNP	denotes	California
T11779	52157-52159	,	denotes	,
T11780	52159-52165	NNP	denotes	United
T11781	52166-52172	NNP	denotes	States
T11782	52172-52173	-RRB-	denotes	)
T11783	52173-52174	.	denotes	.
T11784	52174-52334	sentence	denotes	Protein extraction and detection of Atrx by Western blotting was performed as described previously [4], using the mouse monoclonal anti-ATRX antibody 23C [15].
T11785	52175-52182	NN	denotes	Protein
T11786	52183-52193	NN	denotes	extraction
T11788	52194-52197	CC	denotes	and
T11789	52198-52207	NN	denotes	detection
T11790	52208-52210	IN	denotes	of
T11791	52211-52215	NN	denotes	Atrx
T11792	52216-52218	IN	denotes	by
T11793	52219-52226	NNP	denotes	Western
T11794	52227-52235	NN	denotes	blotting
T11795	52236-52239	VBD	denotes	was
T11787	52240-52249	VBN	denotes	performed
T11796	52250-52252	IN	denotes	as
T11797	52253-52262	VBN	denotes	described
T11798	52263-52273	RB	denotes	previously
T11799	52274-52275	-LRB-	denotes	[
T11800	52275-52276	CD	denotes	4
T11801	52276-52277	-RRB-	denotes	]
T11802	52277-52279	,	denotes	,
T11803	52279-52284	VBG	denotes	using
T11804	52285-52288	DT	denotes	the
T11806	52289-52294	NN	denotes	mouse
T11807	52295-52305	JJ	denotes	monoclonal
T11808	52306-52315	JJ	denotes	anti-ATRX
T11805	52316-52324	NN	denotes	antibody
T11809	52325-52328	NN	denotes	23C
T11810	52329-52330	-LRB-	denotes	[
T11811	52330-52332	CD	denotes	15
T11812	52332-52333	-RRB-	denotes	]
T11813	52333-52334	.	denotes	.
T11814	52334-52401	sentence	denotes	Analyses of cell cycle and apoptosis are described in Protocol S1.
T11815	52335-52343	NNS	denotes	Analyses
T11817	52344-52346	IN	denotes	of
T11818	52347-52351	NN	denotes	cell
T11819	52352-52357	NN	denotes	cycle
T11820	52358-52361	CC	denotes	and
T11821	52362-52371	NN	denotes	apoptosis
T11822	52372-52375	VBP	denotes	are
T11816	52376-52385	VBN	denotes	described
T11823	52386-52388	IN	denotes	in
T11824	52389-52397	NN	denotes	Protocol
T11825	52398-52400	NN	denotes	S1
T11826	52400-52401	.	denotes	.
T11827	52401-52563	sentence	denotes	Methylation of rDNA was analysed in DNA from ES cell clones or from embryoid bodies recovered after 7 d of in vitro differentiation as described previously [41].
T11828	52402-52413	NN	denotes	Methylation
T11830	52414-52416	IN	denotes	of
T11831	52417-52421	NN	denotes	rDNA
T11832	52422-52425	VBD	denotes	was
T11829	52426-52434	VBN	denotes	analysed
T11833	52435-52437	IN	denotes	in
T11834	52438-52441	NN	denotes	DNA
T11835	52442-52446	IN	denotes	from
T11836	52447-52449	NN	denotes	ES
T11837	52450-52454	NN	denotes	cell
T11838	52455-52461	NNS	denotes	clones
T11839	52462-52464	CC	denotes	or
T11840	52465-52469	IN	denotes	from
T11841	52470-52478	JJ	denotes	embryoid
T11842	52479-52485	NNS	denotes	bodies
T11843	52486-52495	VBN	denotes	recovered
T11844	52496-52501	IN	denotes	after
T11845	52502-52503	CD	denotes	7
T11846	52504-52505	NNS	denotes	d
T11847	52506-52508	IN	denotes	of
T11848	52509-52511	FW	denotes	in
T11849	52512-52517	FW	denotes	vitro
T11850	52518-52533	NN	denotes	differentiation
T11851	52534-52536	IN	denotes	as
T11852	52537-52546	VBN	denotes	described
T11853	52547-52557	RB	denotes	previously
T11854	52558-52559	-LRB-	denotes	[
T11855	52559-52561	CD	denotes	41
T11856	52561-52562	-RRB-	denotes	]
T11857	52562-52563	.	denotes	.
T11858	52563-52683	sentence	denotes	Genomic DNA was digested with methylation-sensitive restriction enzymes as described and analysed by Southern blotting.
T11859	52564-52571	JJ	denotes	Genomic
T11860	52572-52575	NN	denotes	DNA
T11862	52576-52579	VBD	denotes	was
T11861	52580-52588	VBN	denotes	digested
T11863	52589-52593	IN	denotes	with
T11864	52594-52605	NN	denotes	methylation
T11866	52605-52606	HYPH	denotes	-
T11865	52606-52615	JJ	denotes	sensitive
T11868	52616-52627	NN	denotes	restriction
T11867	52628-52635	NNS	denotes	enzymes
T11869	52636-52638	IN	denotes	as
T11870	52639-52648	VBN	denotes	described
T11871	52649-52652	CC	denotes	and
T11872	52653-52661	VBN	denotes	analysed
T11873	52662-52664	IN	denotes	by
T11874	52665-52673	NNP	denotes	Southern
T11875	52674-52682	NN	denotes	blotting
T11876	52682-52683	.	denotes	.
T11877	52683-52827	sentence	denotes	The RIB3 and RIB4 probes (which were amplified from human DNA, but cross-react with the mouse rDNA repeat) have been described previously [11].
T11878	52684-52687	DT	denotes	The
T11880	52688-52692	NN	denotes	RIB3
T11881	52693-52696	CC	denotes	and
T11882	52697-52701	NN	denotes	RIB4
T11879	52702-52708	NNS	denotes	probes
T11884	52709-52710	-LRB-	denotes	(
T11885	52710-52715	WDT	denotes	which
T11887	52716-52720	VBD	denotes	were
T11886	52721-52730	VBN	denotes	amplified
T11888	52731-52735	IN	denotes	from
T11889	52736-52741	JJ	denotes	human
T11890	52742-52745	NN	denotes	DNA
T11891	52745-52747	,	denotes	,
T11892	52747-52750	CC	denotes	but
T11893	52751-52762	VBP	denotes	cross-react
T11894	52763-52767	IN	denotes	with
T11895	52768-52771	DT	denotes	the
T11897	52772-52777	NN	denotes	mouse
T11898	52778-52782	NN	denotes	rDNA
T11896	52783-52789	NN	denotes	repeat
T11899	52789-52790	-RRB-	denotes	)
T11900	52791-52795	VBP	denotes	have
T11901	52796-52800	VBN	denotes	been
T11883	52801-52810	VBN	denotes	described
T11902	52811-52821	RB	denotes	previously
T11903	52822-52823	-LRB-	denotes	[
T11904	52823-52825	CD	denotes	11
T11905	52825-52826	-RRB-	denotes	]
T11906	52826-52827	.	denotes	.
T11907	52827-52924	sentence	denotes	Oligonucleotide probes to detect Line 1 and Sine B1 repeats have been described previously [42].
T11908	52828-52843	NN	denotes	Oligonucleotide
T11909	52844-52850	NNS	denotes	probes
T11911	52851-52853	TO	denotes	to
T11912	52854-52860	VB	denotes	detect
T11913	52861-52865	NN	denotes	Line
T11915	52866-52867	CD	denotes	1
T11916	52868-52871	CC	denotes	and
T11917	52872-52876	NN	denotes	Sine
T11918	52877-52879	NN	denotes	B1
T11914	52880-52887	NNS	denotes	repeats
T11919	52888-52892	VBP	denotes	have
T11920	52893-52897	VBN	denotes	been
T11910	52898-52907	VBN	denotes	described
T11921	52908-52918	RB	denotes	previously
T11922	52919-52920	-LRB-	denotes	[
T11923	52920-52922	CD	denotes	42
T11924	52922-52923	-RRB-	denotes	]
T11925	52923-52924	.	denotes	.
T11926	52924-52989	sentence	denotes	The minor satellite probe was a 27-mer oligonucleotide (mCENT2).
T11927	52925-52928	DT	denotes	The
T11929	52929-52934	JJ	denotes	minor
T11930	52935-52944	NN	denotes	satellite
T11928	52945-52950	NN	denotes	probe
T11931	52951-52954	VBD	denotes	was
T11932	52955-52956	DT	denotes	a
T11934	52957-52959	CD	denotes	27
T11936	52959-52960	HYPH	denotes	-
T11935	52960-52963	NN	denotes	mer
T11933	52964-52979	NN	denotes	oligonucleotide
T11937	52980-52981	-LRB-	denotes	(
T11938	52981-52987	NN	denotes	mCENT2
T11939	52987-52988	-RRB-	denotes	)
T11940	52988-52989	.	denotes	.
T11941	52989-53052	sentence	denotes	The major satellite probe was a 27-mer oligonucleotide (DG27).
T11942	52990-52993	DT	denotes	The
T11944	52994-52999	JJ	denotes	major
T11945	53000-53009	NN	denotes	satellite
T11943	53010-53015	NN	denotes	probe
T11946	53016-53019	VBD	denotes	was
T11947	53020-53021	DT	denotes	a
T11949	53022-53024	CD	denotes	27
T11951	53024-53025	HYPH	denotes	-
T11950	53025-53028	NN	denotes	mer
T11948	53029-53044	NN	denotes	oligonucleotide
T11952	53045-53046	-LRB-	denotes	(
T11953	53046-53050	NN	denotes	DG27
T11954	53050-53051	-RRB-	denotes	)
T11955	53051-53052	.	denotes	.
T11956	53052-53229	sentence	denotes	The IAP probe was an ~400 bp PCR product (primers 14A and 13K) amplified from an IAP inserted into the mouse agouti gene [43] and was a gift from Peter Warnecke and Tim Bestor.
T11957	53053-53056	DT	denotes	The
T11959	53057-53060	NN	denotes	IAP
T11958	53061-53066	NN	denotes	probe
T11960	53067-53070	VBD	denotes	was
T11961	53071-53073	DT	denotes	an
T11963	53074-53075	SYM	denotes	~
T11964	53075-53078	CD	denotes	400
T11965	53079-53081	NN	denotes	bp
T11966	53082-53085	NN	denotes	PCR
T11962	53086-53093	NN	denotes	product
T11967	53094-53095	-LRB-	denotes	(
T11968	53095-53102	NNS	denotes	primers
T11969	53103-53106	NN	denotes	14A
T11970	53107-53110	CC	denotes	and
T11971	53111-53114	NN	denotes	13K
T11972	53114-53115	-RRB-	denotes	)
T11973	53116-53125	VBN	denotes	amplified
T11974	53126-53130	IN	denotes	from
T11975	53131-53133	DT	denotes	an
T11976	53134-53137	NN	denotes	IAP
T11977	53138-53146	VBN	denotes	inserted
T11978	53147-53151	IN	denotes	into
T11979	53152-53155	DT	denotes	the
T11981	53156-53161	NN	denotes	mouse
T11982	53162-53168	NN	denotes	agouti
T11980	53169-53173	NN	denotes	gene
T11983	53174-53175	-LRB-	denotes	[
T11984	53175-53177	CD	denotes	43
T11985	53177-53178	-RRB-	denotes	]
T11986	53179-53182	CC	denotes	and
T11987	53183-53186	VBD	denotes	was
T11988	53187-53188	DT	denotes	a
T11989	53189-53193	NN	denotes	gift
T11990	53194-53198	IN	denotes	from
T11991	53199-53204	NNP	denotes	Peter
T11992	53205-53213	NNP	denotes	Warnecke
T11993	53214-53217	CC	denotes	and
T11994	53218-53221	NNP	denotes	Tim
T11995	53222-53228	NNP	denotes	Bestor
T11996	53228-53229	.	denotes	.
T11997	53229-53284	sentence	denotes	The PCR product included the entire 5′ LTR of the IAP.
T11998	53230-53233	DT	denotes	The
T12000	53234-53237	NN	denotes	PCR
T11999	53238-53245	NN	denotes	product
T12001	53246-53254	VBD	denotes	included
T12002	53255-53258	DT	denotes	the
T12004	53259-53265	JJ	denotes	entire
T12005	53266-53267	CD	denotes	5
T12006	53267-53268	SYM	denotes	′
T12003	53269-53272	NN	denotes	LTR
T12007	53273-53275	IN	denotes	of
T12008	53276-53279	DT	denotes	the
T12009	53280-53283	NN	denotes	IAP
T12010	53283-53284	.	denotes	.
T12011	53284-53337	sentence	denotes	All oligonucleotide sequences are shown in Table S1.
T12012	53285-53288	DT	denotes	All
T12014	53289-53304	NN	denotes	oligonucleotide
T12013	53305-53314	NNS	denotes	sequences
T12016	53315-53318	VBP	denotes	are
T12015	53319-53324	VBN	denotes	shown
T12017	53325-53327	IN	denotes	in
T12018	53328-53333	NN	denotes	Table
T12019	53334-53336	NN	denotes	S1
T12020	53336-53337	.	denotes	.
T12080	53339-53349	NN	denotes	Generation
T12081	53350-53352	IN	denotes	of
T12082	53353-53361	NNS	denotes	chimeras
T12083	53361-53363	,	denotes	,
T12084	53363-53369	VBN	denotes	floxed
T12085	53370-53374	NNS	denotes	mice
T12086	53374-53376	,	denotes	,
T12087	53376-53379	CC	denotes	and
T12088	53380-53386	NN	denotes	mutant
T12089	53387-53391	NNS	denotes	mice
T12090	53391-53392	.	denotes	.
T12091	53392-53544	sentence	denotes	Targeted Atrx flox ES cell clones were injected into C57BL/6 blastocysts and transferred into 2.5 dpc pseudopregnant recipients by standard techniques.
T12092	53393-53401	VBN	denotes	Targeted
T12094	53402-53411	NN	denotes	Atrx flox
T12096	53412-53414	NN	denotes	ES
T12095	53415-53419	NN	denotes	cell
T12093	53420-53426	NNS	denotes	clones
T12098	53427-53431	VBD	denotes	were
T12097	53432-53440	VBN	denotes	injected
T12099	53441-53445	IN	denotes	into
T12100	53446-53451	NN	denotes	C57BL
T12102	53451-53452	HYPH	denotes	/
T12103	53452-53453	CD	denotes	6
T12101	53454-53465	NNS	denotes	blastocysts
T12104	53466-53469	CC	denotes	and
T12105	53470-53481	VBN	denotes	transferred
T12106	53482-53486	IN	denotes	into
T12107	53487-53490	CD	denotes	2.5
T12108	53491-53494	NN	denotes	dpc
T12110	53495-53509	JJ	denotes	pseudopregnant
T12109	53510-53520	NNS	denotes	recipients
T12111	53521-53523	IN	denotes	by
T12112	53524-53532	JJ	denotes	standard
T12113	53533-53543	NNS	denotes	techniques
T12114	53543-53544	.	denotes	.
T12115	53544-53623	sentence	denotes	Resulting chimeras were mated with C57BL/6 to establish germline transmission.
T12116	53545-53554	VBG	denotes	Resulting
T12117	53555-53563	NNS	denotes	chimeras
T12119	53564-53568	VBD	denotes	were
T12118	53569-53574	VBN	denotes	mated
T12120	53575-53579	IN	denotes	with
T12121	53580-53585	NN	denotes	C57BL
T12122	53585-53586	HYPH	denotes	/
T12123	53586-53587	CD	denotes	6
T12124	53588-53590	TO	denotes	to
T12125	53591-53600	VB	denotes	establish
T12126	53601-53609	NN	denotes	germline
T12127	53610-53622	NN	denotes	transmission
T12128	53622-53623	.	denotes	.
T12129	53623-53778	sentence	denotes	Offspring with the Atrx flox allele were identified by Southern blotting of SacI-digested tail DNA using the intron 17 probe as shown in Figure 2A and 2C.
T12130	53624-53633	NN	denotes	Offspring
T12132	53634-53638	IN	denotes	with
T12133	53639-53642	DT	denotes	the
T12135	53643-53652	NN	denotes	Atrx flox
T12134	53653-53659	NN	denotes	allele
T12136	53660-53664	VBD	denotes	were
T12131	53665-53675	VBN	denotes	identified
T12137	53676-53678	IN	denotes	by
T12138	53679-53687	NNP	denotes	Southern
T12139	53688-53696	NN	denotes	blotting
T12140	53697-53699	IN	denotes	of
T12141	53700-53704	NN	denotes	SacI
T12143	53704-53705	HYPH	denotes	-
T12142	53705-53713	VBN	denotes	digested
T12145	53714-53718	NN	denotes	tail
T12144	53719-53722	NN	denotes	DNA
T12146	53723-53728	VBG	denotes	using
T12147	53729-53732	DT	denotes	the
T12149	53733-53739	NN	denotes	intron
T12150	53740-53742	CD	denotes	17
T12148	53743-53748	NN	denotes	probe
T12151	53749-53751	IN	denotes	as
T12152	53752-53757	VBN	denotes	shown
T12153	53758-53760	IN	denotes	in
T12154	53761-53767	NN	denotes	Figure
T12155	53768-53770	NN	denotes	2A
T12156	53771-53774	CC	denotes	and
T12157	53775-53777	NN	denotes	2C
T12158	53777-53778	.	denotes	.
T12159	53778-53886	sentence	denotes	For Cre-recombination, Atrx flox mice were crossed with GATA1-Cre transgenic mice as described in the text.
T12160	53779-53782	IN	denotes	For
T12162	53783-53786	NN	denotes	Cre
T12164	53786-53787	HYPH	denotes	-
T12163	53787-53800	NN	denotes	recombination
T12165	53800-53802	,	denotes	,
T12166	53802-53811	NN	denotes	Atrx flox
T12167	53812-53816	NNS	denotes	mice
T12168	53817-53821	VBD	denotes	were
T12161	53822-53829	VBN	denotes	crossed
T12169	53830-53834	IN	denotes	with
T12170	53835-53840	NN	denotes	GATA1
T12172	53840-53841	HYPH	denotes	-
T12171	53841-53844	NN	denotes	Cre
T12173	53845-53855	JJ	denotes	transgenic
T12174	53856-53860	NNS	denotes	mice
T12175	53861-53863	IN	denotes	as
T12176	53864-53873	VBN	denotes	described
T12177	53874-53876	IN	denotes	in
T12178	53877-53880	DT	denotes	the
T12179	53881-53885	NN	denotes	text
T12180	53885-53886	.	denotes	.
T12181	53886-53999	sentence	denotes	Recombinant alleles were detected by Southern blotting as described above or by PCR as described in Protocol S1.
T12182	53887-53898	JJ	denotes	Recombinant
T12183	53899-53906	NNS	denotes	alleles
T12185	53907-53911	VBD	denotes	were
T12184	53912-53920	VBN	denotes	detected
T12186	53921-53923	IN	denotes	by
T12187	53924-53932	NNP	denotes	Southern
T12188	53933-53941	NN	denotes	blotting
T12189	53942-53944	IN	denotes	as
T12190	53945-53954	VBN	denotes	described
T12191	53955-53960	RB	denotes	above
T12192	53961-53963	CC	denotes	or
T12193	53964-53966	IN	denotes	by
T12194	53967-53970	NN	denotes	PCR
T12195	53971-53973	IN	denotes	as
T12196	53974-53983	VBN	denotes	described
T12197	53984-53986	IN	denotes	in
T12198	53987-53995	NN	denotes	Protocol
T12199	53996-53998	NN	denotes	S1
T12200	53998-53999	.	denotes	.
T12292	54001-54021	NN	denotes	Immunohistochemistry
T12293	54021-54023	,	denotes	,
T12294	54023-54025	FW	denotes	in
T12295	54026-54030	FW	denotes	situ
T12296	54031-54044	NN	denotes	hybridisation
T12297	54044-54046	,	denotes	,
T12298	54046-54049	CC	denotes	and
T12299	54050-54055	NN	denotes	TUNEL
T12300	54056-54061	NN	denotes	assay
T12301	54061-54062	.	denotes	.
T12302	54062-54186	sentence	denotes	7.5 dpc decidual swellings were dissected away from maternal tissue and fixed in 4% paraformaldehyde/PBS overnight at 4 °C.
T12303	54063-54066	CD	denotes	7.5
T12304	54067-54070	NN	denotes	dpc
T12306	54071-54079	JJ	denotes	decidual
T12305	54080-54089	NNS	denotes	swellings
T12308	54090-54094	VBD	denotes	were
T12307	54095-54104	VBN	denotes	dissected
T12309	54105-54109	RB	denotes	away
T12310	54110-54114	IN	denotes	from
T12311	54115-54123	JJ	denotes	maternal
T12312	54124-54130	NN	denotes	tissue
T12313	54131-54134	CC	denotes	and
T12314	54135-54140	VBN	denotes	fixed
T12315	54141-54143	IN	denotes	in
T12316	54144-54145	CD	denotes	4
T12317	54145-54146	NN	denotes	%
T12318	54147-54163	NN	denotes	paraformaldehyde
T12319	54163-54164	HYPH	denotes	/
T12320	54164-54167	NN	denotes	PBS
T12321	54168-54177	RB	denotes	overnight
T12322	54178-54180	IN	denotes	at
T12323	54181-54182	CD	denotes	4
T12324	54183-54185	NN	denotes	°C
T12325	54185-54186	.	denotes	.
T12326	54186-54335	sentence	denotes	After embryos were washed thoroughly in PBS, they were dehydrated through an ethanol series and xylene, embedded in paraffin, and sectioned at 5 μm.
T12327	54187-54192	IN	denotes	After
T12329	54193-54200	NNS	denotes	embryos
T12330	54201-54205	VBD	denotes	were
T12328	54206-54212	VBN	denotes	washed
T12332	54213-54223	RB	denotes	thoroughly
T12333	54224-54226	IN	denotes	in
T12334	54227-54230	NN	denotes	PBS
T12335	54230-54232	,	denotes	,
T12336	54232-54236	PRP	denotes	they
T12337	54237-54241	VBD	denotes	were
T12331	54242-54252	VBN	denotes	dehydrated
T12338	54253-54260	IN	denotes	through
T12339	54261-54263	DT	denotes	an
T12341	54264-54271	NN	denotes	ethanol
T12340	54272-54278	NNS	denotes	series
T12342	54279-54282	CC	denotes	and
T12343	54283-54289	NN	denotes	xylene
T12344	54289-54291	,	denotes	,
T12345	54291-54299	VBN	denotes	embedded
T12346	54300-54302	IN	denotes	in
T12347	54303-54311	NN	denotes	paraffin
T12348	54311-54313	,	denotes	,
T12349	54313-54316	CC	denotes	and
T12350	54317-54326	VBN	denotes	sectioned
T12351	54327-54329	IN	denotes	at
T12352	54330-54331	CD	denotes	5
T12353	54332-54334	NNS	denotes	μm
T12354	54334-54335	.	denotes	.
T12355	54335-54526	sentence	denotes	Sections were processed for immunohistochemistry using the ABC Staining System (Santa Cruz Biotechnology, Santa Cruz, California, United States) according to the manufacturer's instructions.
T12356	54336-54344	NNS	denotes	Sections
T12358	54345-54349	VBD	denotes	were
T12357	54350-54359	VBN	denotes	processed
T12359	54360-54363	IN	denotes	for
T12360	54364-54384	NN	denotes	immunohistochemistry
T12361	54385-54390	VBG	denotes	using
T12362	54391-54394	DT	denotes	the
T12364	54395-54398	NNP	denotes	ABC
T12365	54399-54407	NNP	denotes	Staining
T12363	54408-54414	NNP	denotes	System
T12366	54415-54416	-LRB-	denotes	(
T12368	54416-54421	NNP	denotes	Santa
T12369	54422-54426	NNP	denotes	Cruz
T12367	54427-54440	NNP	denotes	Biotechnology
T12370	54440-54442	,	denotes	,
T12371	54442-54447	NNP	denotes	Santa
T12372	54448-54452	NNP	denotes	Cruz
T12373	54452-54454	,	denotes	,
T12374	54454-54464	NNP	denotes	California
T12375	54464-54466	,	denotes	,
T12376	54466-54472	NNP	denotes	United
T12377	54473-54479	NNP	denotes	States
T12378	54479-54480	-RRB-	denotes	)
T12379	54481-54490	VBG	denotes	according
T12380	54491-54493	IN	denotes	to
T12381	54494-54497	DT	denotes	the
T12382	54498-54510	NN	denotes	manufacturer
T12384	54510-54512	POS	denotes	's
T12383	54513-54525	NNS	denotes	instructions
T12385	54525-54526	.	denotes	.
T12386	54526-54824	sentence	denotes	Sections were stained with rabbit polyclonal antibodies against ATRX (H-300, Santa Cruz Biotechnology), Placental lactogen-I (AB1288, Chemicon International, Temecula, California, United States) and phospho (Ser10)-histone H3 (06–570, Upstate Biotechnology, Waltham, Massachusetts, United States).
T12387	54527-54535	NNS	denotes	Sections
T12389	54536-54540	VBD	denotes	were
T12388	54541-54548	VBN	denotes	stained
T12390	54549-54553	IN	denotes	with
T12391	54554-54560	NN	denotes	rabbit
T12393	54561-54571	JJ	denotes	polyclonal
T12392	54572-54582	NNS	denotes	antibodies
T12394	54583-54590	IN	denotes	against
T12395	54591-54595	NN	denotes	ATRX
T12396	54596-54597	-LRB-	denotes	(
T12397	54597-54598	NN	denotes	H
T12398	54598-54599	HYPH	denotes	-
T12399	54599-54602	CD	denotes	300
T12400	54602-54604	,	denotes	,
T12401	54604-54609	NNP	denotes	Santa
T12402	54610-54614	NNP	denotes	Cruz
T12403	54615-54628	NNP	denotes	Biotechnology
T12404	54628-54629	-RRB-	denotes	)
T12405	54629-54631	,	denotes	,
T12406	54631-54640	JJ	denotes	Placental
T12408	54641-54649	NN	denotes	lactogen
T12409	54649-54650	HYPH	denotes	-
T12407	54650-54651	NN	denotes	I
T12410	54652-54653	-LRB-	denotes	(
T12411	54653-54659	NN	denotes	AB1288
T12412	54659-54661	,	denotes	,
T12413	54661-54669	NNP	denotes	Chemicon
T12414	54670-54683	NNP	denotes	International
T12415	54683-54685	,	denotes	,
T12416	54685-54693	NNP	denotes	Temecula
T12417	54693-54695	,	denotes	,
T12418	54695-54705	NNP	denotes	California
T12419	54705-54707	,	denotes	,
T12420	54707-54713	NNP	denotes	United
T12421	54714-54720	NNP	denotes	States
T12422	54720-54721	-RRB-	denotes	)
T12423	54722-54725	CC	denotes	and
T12424	54726-54733	NN	denotes	phospho
T12426	54734-54735	-LRB-	denotes	(
T12427	54735-54740	NN	denotes	Ser10
T12428	54740-54741	-RRB-	denotes	)
T12429	54741-54742	HYPH	denotes	-
T12425	54742-54749	NN	denotes	histone
T12430	54750-54752	NN	denotes	H3
T12431	54753-54754	-LRB-	denotes	(
T12433	54754-54756	CD	denotes	06
T12434	54756-54757	HYPH	denotes	–
T12432	54757-54760	CD	denotes	570
T12435	54760-54762	,	denotes	,
T12436	54762-54769	NNP	denotes	Upstate
T12437	54770-54783	NNP	denotes	Biotechnology
T12438	54783-54785	,	denotes	,
T12439	54785-54792	NNP	denotes	Waltham
T12440	54792-54794	,	denotes	,
T12441	54794-54807	NNP	denotes	Massachusetts
T12442	54807-54809	,	denotes	,
T12443	54809-54815	NNP	denotes	United
T12444	54816-54822	NNP	denotes	States
T12445	54822-54823	-RRB-	denotes	)
T12446	54823-54824	.	denotes	.
T12447	54824-54893	sentence	denotes	Where appropriate, adjacent sections were stained with haematoxylin.
T12448	54825-54830	WRB	denotes	Where
T12449	54831-54842	JJ	denotes	appropriate
T12451	54842-54844	,	denotes	,
T12452	54844-54852	JJ	denotes	adjacent
T12453	54853-54861	NNS	denotes	sections
T12454	54862-54866	VBD	denotes	were
T12450	54867-54874	VBN	denotes	stained
T12455	54875-54879	IN	denotes	with
T12456	54880-54892	NN	denotes	haematoxylin
T12457	54892-54893	.	denotes	.
T12458	54893-55052	sentence	denotes	In some cases, adjacent sections were also analysed to detect apoptotic cells by TUNEL using the in situ cell death detection kit (Roche, Basel, Switzerland).
T12459	54894-54896	IN	denotes	In
T12461	54897-54901	DT	denotes	some
T12462	54902-54907	NNS	denotes	cases
T12463	54907-54909	,	denotes	,
T12464	54909-54917	JJ	denotes	adjacent
T12465	54918-54926	NNS	denotes	sections
T12466	54927-54931	VBD	denotes	were
T12467	54932-54936	RB	denotes	also
T12460	54937-54945	VBN	denotes	analysed
T12468	54946-54948	TO	denotes	to
T12469	54949-54955	VB	denotes	detect
T12470	54956-54965	JJ	denotes	apoptotic
T12471	54966-54971	NNS	denotes	cells
T12472	54972-54974	IN	denotes	by
T12473	54975-54980	NN	denotes	TUNEL
T12474	54981-54986	VBG	denotes	using
T12475	54987-54990	DT	denotes	the
T12477	54991-54993	FW	denotes	in
T12478	54994-54998	FW	denotes	situ
T12479	54999-55003	NN	denotes	cell
T12480	55004-55009	NN	denotes	death
T12481	55010-55019	NN	denotes	detection
T12476	55020-55023	NN	denotes	kit
T12482	55024-55025	-LRB-	denotes	(
T12483	55025-55030	NNP	denotes	Roche
T12484	55030-55032	,	denotes	,
T12485	55032-55037	NNP	denotes	Basel
T12486	55037-55039	,	denotes	,
T12487	55039-55050	NNP	denotes	Switzerland
T12488	55050-55051	-RRB-	denotes	)
T12489	55051-55052	.	denotes	.
T12490	55052-55230	sentence	denotes	After labelling, these slides were mounted in Vectashield containing DAPI (Vector Laboratories, Burlingame, California, United States) and visualised by fluorescence microscopy.
T12491	55053-55058	IN	denotes	After
T12493	55059-55068	NN	denotes	labelling
T12494	55068-55070	,	denotes	,
T12495	55070-55075	DT	denotes	these
T12496	55076-55082	NNS	denotes	slides
T12497	55083-55087	VBD	denotes	were
T12492	55088-55095	VBN	denotes	mounted
T12498	55096-55098	IN	denotes	in
T12499	55099-55110	NNP	denotes	Vectashield
T12500	55111-55121	VBG	denotes	containing
T12501	55122-55126	NN	denotes	DAPI
T12502	55127-55128	-LRB-	denotes	(
T12504	55128-55134	NNP	denotes	Vector
T12503	55135-55147	NNP	denotes	Laboratories
T12505	55147-55149	,	denotes	,
T12506	55149-55159	NNP	denotes	Burlingame
T12507	55159-55161	,	denotes	,
T12508	55161-55171	NNP	denotes	California
T12509	55171-55173	,	denotes	,
T12510	55173-55179	NNP	denotes	United
T12511	55180-55186	NNP	denotes	States
T12512	55186-55187	-RRB-	denotes	)
T12513	55188-55191	CC	denotes	and
T12514	55192-55202	VBN	denotes	visualised
T12515	55203-55205	IN	denotes	by
T12516	55206-55218	NN	denotes	fluorescence
T12517	55219-55229	NN	denotes	microscopy
T12518	55229-55230	.	denotes	.
T12519	55230-55561	sentence	denotes	Whole-mount in situ hybridisations were performed on 8.5 dpc embryos (dissected away from maternal and extraembryonic tissues) using a brachyury (T) riboprobe [16] and on bisected decidual implantation sites from which embryos (8.5 dpc) had been removed using a placental lactogen-1 (Pl-1) riboprobe (see Protocol S1 for details).
T12520	55231-55236	JJ	denotes	Whole
T12522	55236-55237	HYPH	denotes	-
T12521	55237-55242	NN	denotes	mount
T12524	55243-55245	FW	denotes	in
T12525	55246-55250	FW	denotes	situ
T12523	55251-55265	NNS	denotes	hybridisations
T12527	55266-55270	VBD	denotes	were
T12526	55271-55280	VBN	denotes	performed
T12528	55281-55283	IN	denotes	on
T12529	55284-55287	CD	denotes	8.5
T12530	55288-55291	NN	denotes	dpc
T12531	55292-55299	NNS	denotes	embryos
T12532	55300-55301	-LRB-	denotes	(
T12533	55301-55310	VBN	denotes	dissected
T12534	55311-55315	RB	denotes	away
T12535	55316-55320	IN	denotes	from
T12536	55321-55329	JJ	denotes	maternal
T12538	55330-55333	CC	denotes	and
T12539	55334-55348	JJ	denotes	extraembryonic
T12537	55349-55356	NNS	denotes	tissues
T12540	55356-55357	-RRB-	denotes	)
T12541	55358-55363	VBG	denotes	using
T12542	55364-55365	DT	denotes	a
T12544	55366-55375	NN	denotes	brachyury
T12545	55376-55377	-LRB-	denotes	(
T12546	55377-55378	NN	denotes	T
T12547	55378-55379	-RRB-	denotes	)
T12543	55380-55389	NN	denotes	riboprobe
T12548	55390-55391	-LRB-	denotes	[
T12549	55391-55393	CD	denotes	16
T12550	55393-55394	-RRB-	denotes	]
T12551	55395-55398	CC	denotes	and
T12552	55399-55401	IN	denotes	on
T12553	55402-55410	VBN	denotes	bisected
T12555	55411-55419	JJ	denotes	decidual
T12556	55420-55432	NN	denotes	implantation
T12554	55433-55438	NNS	denotes	sites
T12557	55439-55443	IN	denotes	from
T12559	55444-55449	WDT	denotes	which
T12560	55450-55457	NNS	denotes	embryos
T12561	55458-55459	-LRB-	denotes	(
T12563	55459-55462	CD	denotes	8.5
T12562	55463-55466	NN	denotes	dpc
T12564	55466-55467	-RRB-	denotes	)
T12565	55468-55471	VBD	denotes	had
T12566	55472-55476	VBN	denotes	been
T12558	55477-55484	VBN	denotes	removed
T12567	55485-55490	VBG	denotes	using
T12568	55491-55492	DT	denotes	a
T12570	55493-55502	JJ	denotes	placental
T12571	55503-55511	NN	denotes	lactogen
T12572	55511-55512	HYPH	denotes	-
T12573	55512-55513	CD	denotes	1
T12574	55514-55515	-LRB-	denotes	(
T12575	55515-55517	NN	denotes	Pl
T12576	55517-55518	HYPH	denotes	-
T12577	55518-55519	CD	denotes	1
T12578	55519-55520	-RRB-	denotes	)
T12569	55521-55530	NN	denotes	riboprobe
T12579	55531-55532	-LRB-	denotes	(
T12580	55532-55535	VB	denotes	see
T12581	55536-55544	NN	denotes	Protocol
T12582	55545-55547	NN	denotes	S1
T12583	55548-55551	IN	denotes	for
T12584	55552-55559	NNS	denotes	details
T12585	55559-55560	-RRB-	denotes	)
T12586	55560-55561	.	denotes	.
T12623	55563-55573	NN	denotes	Blastocyst
T12624	55574-55583	NN	denotes	outgrowth
T12625	55584-55592	NNS	denotes	cultures
T12626	55592-55593	.	denotes	.
T12627	55593-55787	sentence	denotes	Superovulated heterozygous female mice (Atrx WT/flox) were mated to homozygous GATA1-cre +/+ transgenic males, and blastocysts were flushed from uterine horns with M2 medium (Sigma) at 3.5 dpc.
T12628	55594-55607	VBN	denotes	Superovulated
T12630	55608-55620	JJ	denotes	heterozygous
T12631	55621-55627	JJ	denotes	female
T12629	55628-55632	NNS	denotes	mice
T12633	55633-55634	-LRB-	denotes	(
T12635	55634-55641	NN	denotes	Atrx WT
T12636	55641-55642	HYPH	denotes	/
T12634	55642-55646	NN	denotes	flox
T12637	55646-55647	-RRB-	denotes	)
T12638	55648-55652	VBD	denotes	were
T12632	55653-55658	VBN	denotes	mated
T12639	55659-55661	IN	denotes	to
T12640	55662-55672	JJ	denotes	homozygous
T12642	55673-55678	NN	denotes	GATA1
T12644	55678-55679	HYPH	denotes	-
T12643	55679-55682	NN	denotes	cre
T12645	55683-55684	SYM	denotes	+
T12646	55684-55685	HYPH	denotes	/
T12647	55685-55686	SYM	denotes	+
T12648	55687-55697	JJ	denotes	transgenic
T12641	55698-55703	NNS	denotes	males
T12649	55703-55705	,	denotes	,
T12650	55705-55708	CC	denotes	and
T12651	55709-55720	NNS	denotes	blastocysts
T12653	55721-55725	VBD	denotes	were
T12652	55726-55733	VBN	denotes	flushed
T12654	55734-55738	IN	denotes	from
T12655	55739-55746	JJ	denotes	uterine
T12656	55747-55752	NNS	denotes	horns
T12657	55753-55757	IN	denotes	with
T12658	55758-55760	NN	denotes	M2
T12659	55761-55767	NN	denotes	medium
T12660	55768-55769	-LRB-	denotes	(
T12661	55769-55774	NNP	denotes	Sigma
T12662	55774-55775	-RRB-	denotes	)
T12663	55776-55778	IN	denotes	at
T12664	55779-55782	CD	denotes	3.5
T12665	55783-55786	NNS	denotes	dpc
T12666	55786-55787	.	denotes	.
T12667	55787-55890	sentence	denotes	Individual blastocysts were cultured in multiwell tissue cultures plates as described previously [44].
T12668	55788-55798	JJ	denotes	Individual
T12669	55799-55810	NNS	denotes	blastocysts
T12671	55811-55815	VBD	denotes	were
T12670	55816-55824	VBN	denotes	cultured
T12672	55825-55827	IN	denotes	in
T12673	55828-55837	JJ	denotes	multiwell
T12675	55838-55844	NN	denotes	tissue
T12674	55845-55853	NNS	denotes	cultures
T12676	55854-55860	NNS	denotes	plates
T12677	55861-55863	IN	denotes	as
T12678	55864-55873	VBN	denotes	described
T12679	55874-55884	RB	denotes	previously
T12680	55885-55886	-LRB-	denotes	[
T12681	55886-55888	CD	denotes	44
T12682	55888-55889	-RRB-	denotes	]
T12683	55889-55890	.	denotes	.
T12684	55890-55973	sentence	denotes	Cultures were inspected and photographed daily and the extent of outgrowth scored.
T12685	55891-55899	NNS	denotes	Cultures
T12687	55900-55904	VBD	denotes	were
T12686	55905-55914	VBN	denotes	inspected
T12688	55915-55918	CC	denotes	and
T12689	55919-55931	VBN	denotes	photographed
T12690	55932-55937	RB	denotes	daily
T12691	55938-55941	CC	denotes	and
T12692	55942-55945	DT	denotes	the
T12693	55946-55952	NN	denotes	extent
T12695	55953-55955	IN	denotes	of
T12696	55956-55965	NN	denotes	outgrowth
T12694	55966-55972	VBN	denotes	scored
T12697	55972-55973	.	denotes	.
T12698	55973-56027	sentence	denotes	After 7 d, cultures were harvested and DNA extracted.
T12699	55974-55979	IN	denotes	After
T12701	55980-55981	CD	denotes	7
T12702	55982-55983	NNS	denotes	d
T12703	55983-55985	,	denotes	,
T12704	55985-55993	NNS	denotes	cultures
T12705	55994-55998	VBD	denotes	were
T12700	55999-56008	VBN	denotes	harvested
T12706	56009-56012	CC	denotes	and
T12707	56013-56016	NN	denotes	DNA
T12708	56017-56026	VBN	denotes	extracted
T12709	56026-56027	.	denotes	.
T12710	56027-56120	sentence	denotes	The Atrx genotype and sex of each culture was determined by PCR as described in Protocol S1.
T12711	56028-56031	DT	denotes	The
T12713	56032-56036	NN	denotes	Atrx
T12712	56037-56045	NN	denotes	genotype
T12715	56046-56049	CC	denotes	and
T12716	56050-56053	NN	denotes	sex
T12717	56054-56056	IN	denotes	of
T12718	56057-56061	DT	denotes	each
T12719	56062-56069	NN	denotes	culture
T12720	56070-56073	VBD	denotes	was
T12714	56074-56084	VBN	denotes	determined
T12721	56085-56087	IN	denotes	by
T12722	56088-56091	NN	denotes	PCR
T12723	56092-56094	IN	denotes	as
T12724	56095-56104	VBN	denotes	described
T12725	56105-56107	IN	denotes	in
T12726	56108-56116	NN	denotes	Protocol
T12727	56117-56119	NN	denotes	S1
T12728	56119-56120	.	denotes	.
R1	T152	T153	nsubj	Loss,Affects
R10	T164	T162	pobj	Inactivation,of
R100	T261	T262	det	the,stage
R1000	T1755	T1753	dobj	clones,recovered
R1001	T1756	T1757	prep	in,flanked
R1002	T1757	T1755	relcl	flanked,clones
R1003	T1758	T1756	pobj	which,in
R1004	T1759	T1757	nsubjpass	exon,flanked
R1005	T1760	T1759	nummod	18,exon
R1006	T1761	T1757	aux	has,flanked
R1007	T1762	T1757	auxpass	been,flanked
R1008	T1763	T1757	agent	by,flanked
R1009	T1764	T1765	compound	loxP,sites
R101	T262	T260	pobj	stage,at
R1010	T1765	T1763	pobj	sites,by
R1011	T1766	T1765	compound	recognition,sites
R1012	T1767	T1765	prep	for,sites
R1013	T1768	T1769	det	the,recombinase
R1014	T1769	T1767	pobj	recombinase,for
R1015	T1770	T1769	compound	Cre,recombinase
R1016	T1771	T1772	punct	(,allele
R1017	T1772	T1769	parataxis	allele,recombinase
R1018	T1773	T1772	compound	Atrx flox,allele
R1019	T1774	T1772	prep	in,allele
R102	T263	T264	quantmod	8,16
R1020	T1775	T1776	compound	Figure,2A
R1021	T1776	T1774	pobj	2A,in
R1022	T1777	T1772	punct	),allele
R1023	T1778	T1779	punct	(,2B
R1024	T1779	T1753	parataxis	2B,recovered
R1025	T1780	T1779	compound	Figure,2B
R1026	T1781	T1779	punct	),2B
R1027	T1782	T1739	punct	.,adopted
R1028	T1784	T1785	det	This,allele
R1029	T1785	T1786	nsubj	allele,contains
R103	T264	T267	nummod	16,cell
R1030	T1787	T1786	advmod	also,contains
R1031	T1788	T1789	det	a,cassette
R1032	T1789	T1786	dobj	cassette,contains
R1033	T1790	T1791	npadvmod	loxP,flanked
R1034	T1791	T1789	amod	flanked,cassette
R1035	T1792	T1791	punct	-,flanked
R1036	T1793	T1794	compound	MC1,neor
R1037	T1794	T1789	compound	neor,cassette
R1038	T1795	T1794	punct	-,neor
R1039	T1796	T1786	prep	in,contains
R104	T265	T264	punct	-,16
R1040	T1797	T1796	pobj	intron,in
R1041	T1798	T1797	nummod	17,intron
R1042	T1799	T1800	punct	(,2A
R1043	T1800	T1786	parataxis	2A,contains
R1044	T1801	T1800	compound	Figure,2A
R1045	T1802	T1800	punct	),2A
R1046	T1803	T1786	punct	.,contains
R1047	T1805	T1806	nmod	Northern,blot
R1048	T1806	T1809	compound	blot,analyses
R1049	T1807	T1805	cc	and,Northern
R105	T266	T264	quantmod	to,16
R1050	T1808	T1805	conj	Western,Northern
R1051	T1809	T1810	nsubj	analyses,confirmed
R1052	T1811	T1812	punct	(,2D
R1053	T1812	T1809	parataxis	2D,analyses
R1054	T1813	T1812	compound	Figure,2D
R1055	T1814	T1812	cc	and,2D
R1056	T1815	T1812	conj	2E,2D
R1057	T1816	T1812	punct	),2D
R1058	T1817	T1818	mark	that,continued
R1059	T1818	T1810	ccomp	continued,confirmed
R106	T267	T262	compound	cell,stage
R1060	T1819	T1820	det	the,clones
R1061	T1820	T1818	nsubj	clones,continued
R1062	T1821	T1820	compound	Atrx flox,clones
R1063	T1822	T1823	aux	to,express
R1064	T1823	T1818	xcomp	express,continued
R1065	T1824	T1825	preconj	both,protein
R1066	T1825	T1823	dobj	protein,express
R1067	T1826	T1827	amod	full,length
R1068	T1827	T1825	compound	length,protein
R1069	T1828	T1827	punct	-,length
R107	T268	T267	punct	-,cell
R1070	T1829	T1825	compound	Atrx,protein
R1071	T1830	T1825	cc	and,protein
R1072	T1831	T1832	det	the,isoform
R1073	T1832	T1825	conj	isoform,protein
R1074	T1833	T1832	amod	truncated,isoform
R1075	T1834	T1832	compound	Atrxt,isoform
R1076	T1835	T1810	punct	.,confirmed
R1077	T1837	T1838	aux	To,generate
R1078	T1838	T1839	advcl	generate,transfected
R1079	T1840	T1841	det	the,deletion
R108	T269	T262	prep	of,stage
R1080	T1841	T1838	dobj	deletion,generate
R1081	T1842	T1841	amod	full,deletion
R1082	T1843	T1838	prep	in,generate
R1083	T1844	T1845	compound	ES,cells
R1084	T1845	T1843	pobj	cells,in
R1085	T1846	T1839	punct	", ",transfected
R1086	T1847	T1848	det	the,clones
R1087	T1848	T1839	nsubjpass	clones,transfected
R1088	T1849	T1848	compound	Atrx flox,clones
R1089	T1850	T1851	punct	(,F12
R109	T270	T269	pobj	development,of
R1090	T1851	T1848	parataxis	F12,clones
R1091	T1852	T1851	nummod	1,F12
R1092	T1853	T1851	punct	/,F12
R1093	T1854	T1851	cc	and,F12
R1094	T1855	T1856	nummod	1,G11
R1095	T1856	T1851	conj	G11,F12
R1096	T1857	T1856	punct	/,G11
R1097	T1858	T1851	punct	),F12
R1098	T1859	T1839	auxpass	were,transfected
R1099	T1860	T1839	advmod	transiently,transfected
R11	T165	T164	punct	-,Inactivation
R110	T271	T223	punct	.,inactivated
R1100	T1861	T1839	prep	with,transfected
R1101	T1862	T1863	det	a,plasmid
R1102	T1863	T1861	pobj	plasmid,with
R1103	T1864	T1865	compound	Cre,recombinase
R1104	T1865	T1863	compound	recombinase,plasmid
R1105	T1866	T1865	punct	-,recombinase
R1106	T1867	T1863	compound	expression,plasmid
R1107	T1868	T1863	punct	(,plasmid
R1108	T1869	T1870	compound	pCAGGS,IRESpuro
R1109	T1870	T1863	appos	IRESpuro,plasmid
R111	T273	T274	det	The,protein
R1110	T1871	T1870	punct	-,IRESpuro
R1111	T1872	T1870	compound	Cre,IRESpuro
R1112	T1873	T1870	punct	-,IRESpuro
R1113	T1874	T1839	punct	),transfected
R1114	T1875	T1839	punct	", ",transfected
R1115	T1876	T1839	cc	and,transfected
R1116	T1877	T1878	nsubjpass	subclones,bearing
R1117	T1878	T1839	conj	bearing,transfected
R1118	T1879	T1878	auxpass	were,bearing
R1119	T1880	T1878	aux	recovered,bearing
R112	T274	T275	nsubjpass	protein,expressed
R1120	T1881	T1882	det	an,allele
R1121	T1882	T1878	dobj	allele,bearing
R1122	T1883	T1884	punct	(,Atrx Δ18Δneo
R1123	T1884	T1882	parataxis	Atrx Δ18Δneo,allele
R1124	T1885	T1884	prep	in,Atrx Δ18Δneo
R1125	T1886	T1887	compound	Figure,2A
R1126	T1887	T1885	pobj	2A,in
R1127	T1888	T1884	punct	),Atrx Δ18Δneo
R1128	T1889	T1890	prep	in,deleted
R1129	T1890	T1882	relcl	deleted,allele
R113	T276	T274	punct	", ",protein
R1130	T1891	T1889	pobj	which,in
R1131	T1892	T1893	preconj	both,exon
R1132	T1893	T1890	nsubjpass	exon,deleted
R1133	T1894	T1893	nummod	18,exon
R1134	T1895	T1893	cc	and,exon
R1135	T1896	T1897	det	the,cassette
R1136	T1897	T1893	conj	cassette,exon
R1137	T1898	T1897	compound	neor,cassette
R1138	T1899	T1890	aux	had,deleted
R1139	T1900	T1890	auxpass	been,deleted
R114	T277	T274	appos	Atrx,protein
R1140	T1901	T1890	agent	by,deleted
R1141	T1902	T1903	det	the,recombinase
R1142	T1903	T1901	pobj	recombinase,by
R1143	T1904	T1903	compound	Cre,recombinase
R1144	T1905	T1906	punct	(,resulting
R1145	T1906	T1890	parataxis	resulting,deleted
R1146	T1907	T1906	prep	from,resulting
R1147	T1908	T1909	det	the,event
R1148	T1909	T1907	pobj	event,from
R1149	T1910	T1909	compound	recombination,event
R115	T278	T275	punct	", ",expressed
R1150	T1911	T1909	acl	labelled,event
R1151	T1912	T1913	punct	“,C
R1152	T1913	T1911	oprd	C,labelled
R1153	T1914	T1911	punct	”,labelled
R1154	T1915	T1911	prep	in,labelled
R1155	T1916	T1917	det	the,allele
R1156	T1917	T1915	pobj	allele,in
R1157	T1918	T1917	compound	Atrx flox,allele
R1158	T1919	T1917	acl	shown,allele
R1159	T1920	T1919	prep	in,shown
R116	T279	T275	auxpass	was,expressed
R1160	T1921	T1922	compound	Figure,2A
R1161	T1922	T1920	pobj	2A,in
R1162	T1923	T1906	punct	),resulting
R1163	T1924	T1925	punct	(,2C
R1164	T1925	T1890	parataxis	2C,deleted
R1165	T1926	T1925	compound	Figure,2C
R1166	T1927	T1925	punct	),2C
R1167	T1928	T1878	punct	.,bearing
R1168	T1930	T1931	nmod	Northern,blot
R1169	T1931	T1934	compound	blot,analyses
R117	T280	T275	advmod	ubiquitously,expressed
R1170	T1932	T1930	cc	and,Northern
R1171	T1933	T1930	conj	Western,Northern
R1172	T1934	T1935	nsubj	analyses,revealed
R1173	T1936	T1937	punct	(,2D
R1174	T1937	T1934	parataxis	2D,analyses
R1175	T1938	T1937	compound	Figure,2D
R1176	T1939	T1937	cc	and,2D
R1177	T1940	T1937	conj	2E,2D
R1178	T1941	T1937	punct	),2D
R1179	T1942	T1943	mark	that,abolished
R118	T281	T275	punct	", ",expressed
R1180	T1943	T1935	ccomp	abolished,revealed
R1181	T1944	T1945	det	the,transcript
R1182	T1945	T1943	nsubjpass	transcript,abolished
R1183	T1946	T1947	amod	full,length
R1184	T1947	T1945	compound	length,transcript
R1185	T1948	T1947	punct	-,length
R1186	T1949	T1945	compound	Atrx,transcript
R1187	T1950	T1945	cc	and,transcript
R1188	T1951	T1945	conj	protein,transcript
R1189	T1952	T1943	auxpass	is,abolished
R119	T282	T275	cc	and,expressed
R1190	T1953	T1943	advmod	completely,abolished
R1191	T1954	T1943	prep	in,abolished
R1192	T1955	T1956	det	the,clones
R1193	T1956	T1954	pobj	clones,in
R1194	T1957	T1958	npadvmod	Atrx Δ18Δneo,recombinant
R1195	T1958	T1956	amod	recombinant,clones
R1196	T1959	T1943	punct	", ",abolished
R1197	T1960	T1943	advcl	suggesting,abolished
R1198	T1961	T1962	mark	that,has
R1199	T1962	T1960	ccomp	has,suggesting
R12	T166	T153	prep	in,Affects
R120	T283	T284	amod	male,embryos
R1200	T1963	T1962	nsubj	deletion,has
R1201	T1964	T1963	prep	of,deletion
R1202	T1965	T1966	det	this,region
R1203	T1966	T1964	pobj	region,of
R1204	T1967	T1968	det	a,effect
R1205	T1968	T1962	dobj	effect,has
R1206	T1969	T1970	advmod	highly,destabilising
R1207	T1970	T1968	amod	destabilising,effect
R1208	T1971	T1962	prep	on,has
R1209	T1972	T1973	det	the,transcript
R121	T284	T285	nsubj	embryos,implanted
R1210	T1973	T1971	pobj	transcript,on
R1211	T1974	T1975	amod	full,length
R1212	T1975	T1973	compound	length,transcript
R1213	T1976	T1975	punct	-,length
R1214	T1977	T1935	punct	.,revealed
R1215	T1979	T1980	mark	As,expected
R1216	T1980	T1981	advcl	expected,is
R1217	T1982	T1981	punct	", ",is
R1218	T1983	T1984	det	the,isoform
R1219	T1984	T1981	nsubj	isoform,is
R122	T285	T275	conj	implanted,expressed
R1220	T1985	T1984	amod	truncated,isoform
R1221	T1986	T1984	compound	Atrxt,isoform
R1222	T1987	T1984	punct	", ",isoform
R1223	T1988	T1989	det	the,transcript
R1224	T1989	T1990	dep	transcript,terminated
R1225	T1990	T1984	relcl	terminated,isoform
R1226	T1991	T1989	prep	of,transcript
R1227	T1992	T1991	pobj	which,of
R1228	T1993	T1990	auxpass	is,terminated
R1229	T1994	T1990	prep	within,terminated
R123	T286	T284	amod	null,embryos
R1230	T1995	T1994	pobj	intron,within
R1231	T1996	T1995	nummod	11,intron
R1232	T1997	T1998	punct	[,4
R1233	T1998	T1990	parataxis	4,terminated
R1234	T1999	T1998	punct	],4
R1235	T2000	T1981	punct	", ",is
R1236	T2001	T1981	acomp	unaffected,is
R1237	T2002	T2001	prep	by,unaffected
R1238	T2003	T2004	det	the,deletion
R1239	T2004	T2002	pobj	deletion,by
R124	T287	T286	prep	for,null
R1240	T2005	T2004	prep	of,deletion
R1241	T2006	T2005	pobj	exon,of
R1242	T2007	T2006	nummod	18,exon
R1243	T2008	T2009	punct	(,2E
R1244	T2009	T1981	parataxis	2E,is
R1245	T2010	T2009	compound	Figure,2E
R1246	T2011	T2009	punct	),2E
R1247	T2012	T1981	punct	.,is
R1248	T2014	T2015	mark	While,is
R1249	T2015	T2020	advcl	is,is
R125	T288	T287	pobj	Atrx,for
R1250	T2016	T2017	det	the,function
R1251	T2017	T2015	nsubj	function,is
R1252	T2018	T2017	prep	of,function
R1253	T2019	T2018	pobj	Atrxt,of
R1254	T2021	T2015	neg	not,is
R1255	T2022	T2015	advmod	yet,is
R1256	T2023	T2015	acomp	clear,is
R1257	T2024	T2020	punct	", ",is
R1258	T2025	T2026	det	this,isoform
R1259	T2026	T2020	nsubj	isoform,is
R126	T289	T285	cc	and,implanted
R1260	T2027	T2026	punct	", ",isoform
R1261	T2028	T2029	dep	which,contains
R1262	T2029	T2026	relcl	contains,isoform
R1263	T2030	T2031	det	the,domain
R1264	T2031	T2029	dobj	domain,contains
R1265	T2032	T2033	npadvmod	PHD,like
R1266	T2033	T2031	amod	like,domain
R1267	T2034	T2033	punct	-,like
R1268	T2035	T2031	cc	but,domain
R1269	T2036	T2035	neg	not,but
R127	T290	T285	conj	gastrulated,implanted
R1270	T2037	T2038	det	the,motifs
R1271	T2038	T2031	conj	motifs,domain
R1272	T2039	T2040	compound	SWI,SNF
R1273	T2040	T2038	compound	SNF,motifs
R1274	T2041	T2040	punct	/,SNF
R1275	T2042	T2043	punct	(,Figure
R1276	T2043	T2029	parataxis	Figure,contains
R1277	T2044	T2043	nummod	1,Figure
R1278	T2045	T2043	punct	),Figure
R1279	T2046	T2020	punct	", ",is
R128	T291	T285	advmod	normally,implanted
R1280	T2047	T2020	acomp	unlikely,is
R1281	T2048	T2049	aux	to,be
R1282	T2049	T2047	xcomp	be,unlikely
R1283	T2050	T2051	advmod	functionally,equivalent
R1284	T2051	T2049	acomp	equivalent,be
R1285	T2052	T2051	prep	to,equivalent
R1286	T2053	T2054	det	the,protein
R1287	T2054	T2052	pobj	protein,to
R1288	T2055	T2056	amod	full,length
R1289	T2056	T2054	compound	length,protein
R129	T292	T285	cc	but,implanted
R1290	T2057	T2056	punct	-,length
R1291	T2058	T2020	punct	.,is
R1292	T2060	T2061	advmod	Thus,allowed
R1293	T2062	T2061	punct	", ",allowed
R1294	T2063	T2064	det	a,strategy
R1295	T2064	T2061	nsubj	strategy,allowed
R1296	T2065	T2066	amod	conditional,knockout
R1297	T2066	T2064	compound	knockout,strategy
R1298	T2067	T2068	det	the,isolation
R1299	T2068	T2061	dobj	isolation,allowed
R13	T167	T168	amod	Extraembryonic,Tissues
R130	T293	T294	aux	did,survive
R1300	T2069	T2068	prep	of,isolation
R1301	T2070	T2071	compound	ES,cells
R1302	T2071	T2069	pobj	cells,of
R1303	T2072	T2073	dep	that,are
R1304	T2073	T2068	relcl	are,isolation
R1305	T2074	T2073	acomp	null,are
R1306	T2075	T2074	prep	for,null
R1307	T2076	T2077	amod	full,length
R1308	T2077	T2079	compound	length,Atrx
R1309	T2078	T2077	punct	-,length
R131	T294	T285	conj	survive,implanted
R1310	T2079	T2075	pobj	Atrx,for
R1311	T2080	T2061	punct	.,allowed
R1313	T2534	T2535	amod	Perturbed,Growth
R1314	T2536	T2535	cc	and,Growth
R1315	T2537	T2538	compound	Methylation,Defects
R1316	T2538	T2535	conj	Defects,Growth
R1317	T2539	T2535	prep	in,Growth
R1318	T2540	T2541	amod	Atrxnull,Cells
R1319	T2541	T2539	pobj	Cells,in
R132	T295	T294	neg	not,survive
R1320	T2542	T2541	compound	ES,Cells
R1321	T2544	T2545	amod	Atrxnull,cells
R1322	T2545	T2547	nsubjpass	cells,maintained
R1323	T2546	T2545	compound	ES,cells
R1324	T2548	T2547	aux	could,maintained
R1325	T2549	T2547	auxpass	be,maintained
R1326	T2550	T2547	prep	in,maintained
R1327	T2551	T2550	pobj	culture,in
R1328	T2552	T2547	cc	but,maintained
R1329	T2553	T2547	conj	were,maintained
R133	T296	T294	prep	beyond,survive
R1330	T2554	T2553	advmod	generally,were
R1331	T2555	T2556	advmod	slower,growing
R1332	T2556	T2553	acomp	growing,were
R1333	T2557	T2556	prep	than,growing
R1334	T2558	T2559	nmod	Atrx,ES
R1335	T2559	T2561	compound	ES,clones
R1336	T2560	T2559	punct	+,ES
R1337	T2561	T2557	pobj	clones,than
R1338	T2562	T2553	punct	", ",were
R1339	T2563	T2553	cc	and,were
R134	T297	T298	nummod	9.5,days
R1340	T2564	T2553	conj	appeared,were
R1341	T2565	T2566	aux	to,undergo
R1342	T2566	T2564	xcomp	undergo,appeared
R1343	T2567	T2568	amod	higher,rates
R1344	T2568	T2566	dobj	rates,undergo
R1345	T2569	T2568	prep	of,rates
R1346	T2570	T2571	amod	spontaneous,differentiation
R1347	T2571	T2569	pobj	differentiation,of
R1348	T2572	T2547	punct	.,maintained
R1349	T2574	T2575	nsubj	We,investigated
R135	T298	T296	pobj	days,beyond
R1350	T2576	T2575	advmod	directly,investigated
R1351	T2577	T2578	det	the,effect
R1352	T2578	T2575	dobj	effect,investigated
R1353	T2579	T2578	prep	of,effect
R1354	T2580	T2579	pobj	Atrx,of
R1355	T2581	T2578	prep	on,effect
R1356	T2582	T2583	compound	ES,cell
R1357	T2583	T2584	compound	cell,growth
R1358	T2584	T2581	pobj	growth,on
R1359	T2585	T2575	prep	by,investigated
R136	T299	T298	advmod	postcoitus,days
R1360	T2586	T2585	pcomp	comparing,by
R1361	T2587	T2588	nmod	Atrx,clones
R1362	T2588	T2586	dobj	clones,comparing
R1363	T2589	T2587	punct	+,Atrx
R1364	T2590	T2587	cc	and,Atrx
R1365	T2591	T2587	conj	Atrxnull,Atrx
R1366	T2592	T2593	compound	ES,cell
R1367	T2593	T2588	compound	cell,clones
R1368	T2594	T2586	prep	in,comparing
R1369	T2595	T2596	compound	competition,cultures
R137	T300	T294	prep	due,survive
R1370	T2596	T2594	pobj	cultures,in
R1371	T2597	T2575	punct	.,investigated
R1372	T2599	T2600	amod	Equal,numbers
R1373	T2600	T2601	nsubjpass	numbers,inoculated
R1374	T2602	T2600	prep	of,numbers
R1375	T2603	T2604	nmod	Atrx,cells
R1376	T2604	T2602	pobj	cells,of
R1377	T2605	T2603	punct	+,Atrx
R1378	T2606	T2603	punct	(,Atrx
R1379	T2607	T2603	acl	bearing,Atrx
R138	T301	T300	pcomp	to,due
R1380	T2608	T2609	preconj	either,Atrx WT
R1381	T2609	T2607	dobj	Atrx WT,bearing
R1382	T2610	T2609	det	an,Atrx WT
R1383	T2611	T2609	cc	or,Atrx WT
R1384	T2612	T2613	det	an,Atrx flox
R1385	T2613	T2614	compound	Atrx flox,allele
R1386	T2614	T2609	conj	allele,Atrx WT
R1387	T2615	T2603	punct	),Atrx
R1388	T2616	T2603	cc	and,Atrx
R1389	T2617	T2603	conj	Atrxnull,Atrx
R139	T302	T303	det	a,defect
R1390	T2618	T2617	punct	(,Atrxnull
R1391	T2619	T2617	acl	bearing,Atrxnull
R1392	T2620	T2621	det	an,allele
R1393	T2621	T2619	dobj	allele,bearing
R1394	T2622	T2621	compound	Atrx Δ18Δneo,allele
R1395	T2623	T2604	punct	),cells
R1396	T2624	T2604	compound	ES,cells
R1397	T2625	T2601	auxpass	were,inoculated
R1398	T2626	T2601	prep	into,inoculated
R1399	T2627	T2626	pobj	cultures,into
R14	T168	T166	pobj	Tissues,in
R140	T303	T300	pobj	defect,due
R1400	T2628	T2601	cc	and,inoculated
R1401	T2629	T2630	det	the,cultures
R1402	T2630	T2632	nsubjpass	cultures,passaged
R1403	T2631	T2630	amod	mixed,cultures
R1404	T2632	T2601	conj	passaged,inoculated
R1405	T2633	T2632	auxpass	were,passaged
R1406	T2634	T2635	punct	(,split
R1407	T2635	T2632	parataxis	split,passaged
R1408	T2636	T2635	nummod	1,split
R1409	T2637	T2638	punct	:,3
R141	T304	T303	prep	in,defect
R1410	T2638	T2636	prep	3,1
R1411	T2639	T2635	punct	),split
R1412	T2640	T2641	det	every,d
R1413	T2641	T2632	npadvmod	d,passaged
R1414	T2642	T2641	nummod	2,d
R1415	T2643	T2632	prep	for,passaged
R1416	T2644	T2645	quantmod	8,10
R1417	T2645	T2647	nummod	10,d
R1418	T2646	T2645	punct	–,10
R1419	T2647	T2643	pobj	d,for
R142	T305	T304	pobj	formation,in
R1420	T2648	T2632	punct	.,passaged
R1421	T2650	T2651	det	The,abundance
R1422	T2651	T2653	nsubjpass	abundance,analysed
R1423	T2652	T2651	amod	relative,abundance
R1424	T2654	T2651	prep	of,abundance
R1425	T2655	T2656	det	the,alleles
R1426	T2656	T2654	pobj	alleles,of
R1427	T2657	T2656	amod	different,alleles
R1428	T2658	T2651	prep	in,abundance
R1429	T2659	T2660	det	the,culture
R143	T306	T305	prep	of,formation
R1430	T2660	T2658	pobj	culture,in
R1431	T2661	T2651	prep	at,abundance
R1432	T2662	T2663	det	each,point
R1433	T2663	T2661	pobj	point,at
R1434	T2664	T2663	compound	time,point
R1435	T2665	T2653	auxpass	was,analysed
R1436	T2666	T2653	prep	by,analysed
R1437	T2667	T2668	compound	Southern,blotting
R1438	T2668	T2666	pobj	blotting,by
R1439	T2669	T2670	punct	(,3A
R144	T307	T308	det	the,trophoblast
R1440	T2670	T2653	parataxis	3A,analysed
R1441	T2671	T2670	compound	Figure,3A
R1442	T2672	T2670	punct	),3A
R1443	T2673	T2653	punct	.,analysed
R1444	T2675	T2676	det	The,clone
R1445	T2676	T2677	nsubjpass	clone,outgrown
R1446	T2678	T2676	acl	containing,clone
R1447	T2679	T2680	det	the,allele
R1448	T2680	T2678	dobj	allele,containing
R1449	T2681	T2680	compound	Atrx Δ18Δneo,allele
R145	T308	T306	pobj	trophoblast,of
R1450	T2682	T2677	auxpass	was,outgrown
R1451	T2683	T2677	advmod	rapidly,outgrown
R1452	T2684	T2677	agent	by,outgrown
R1453	T2685	T2686	preconj	both,cells
R1454	T2686	T2684	pobj	cells,by
R1455	T2687	T2686	compound	Atrx WT,cells
R1456	T2688	T2686	compound	ES,cells
R1457	T2689	T2686	cc	and,cells
R1458	T2690	T2686	conj	cells,cells
R1459	T2691	T2690	acl	bearing,cells
R146	T309	T308	amod	extraembryonic,trophoblast
R1460	T2692	T2693	det	the,allele
R1461	T2693	T2691	dobj	allele,bearing
R1462	T2694	T2693	compound	Atrx flox,allele
R1463	T2695	T2677	punct	.,outgrown
R1464	T2697	T2698	prep	In,continued
R1465	T2699	T2700	det	a,experiment
R1466	T2700	T2697	pobj	experiment,In
R1467	T2701	T2700	compound	control,experiment
R1468	T2702	T2700	compound	competition,experiment
R1469	T2703	T2700	prep	between,experiment
R147	T310	T308	punct	", ",trophoblast
R1470	T2704	T2705	amod	different,clones
R1471	T2705	T2703	pobj	clones,between
R1472	T2706	T2705	acl	bearing,clones
R1473	T2707	T2708	amod	functional,alleles
R1474	T2708	T2706	dobj	alleles,bearing
R1475	T2709	T2708	compound	Atrx,alleles
R1476	T2710	T2708	punct	(,alleles
R1477	T2711	T2708	appos	Atrx WT,alleles
R1478	T2712	T2711	cc	and,Atrx WT
R1479	T2713	T2711	conj	Atrx flox,Atrx WT
R148	T311	T308	appos	one,trophoblast
R1480	T2714	T2698	punct	),continued
R1481	T2715	T2698	punct	", ",continued
R1482	T2716	T2717	det	both,clones
R1483	T2717	T2698	nsubj	clones,continued
R1484	T2718	T2719	aux	to,be
R1485	T2719	T2698	xcomp	be,continued
R1486	T2720	T2721	advmod	equally,represented
R1487	T2721	T2719	acomp	represented,be
R1488	T2722	T2719	prep	after,be
R1489	T2723	T2724	nummod	8,d
R149	T312	T311	prep	of,one
R1490	T2724	T2722	pobj	d,after
R1491	T2725	T2724	prep	of,d
R1492	T2726	T2725	pobj	cocultivation,of
R1493	T2727	T2698	punct	.,continued
R1494	T2729	T2730	advmod	Thus,suggested
R1495	T2731	T2730	punct	", ",suggested
R1496	T2732	T2733	mark	although,recovered
R1497	T2733	T2730	advcl	recovered,suggested
R1498	T2734	T2735	amod	Atrxnull,cells
R1499	T2735	T2733	nsubj	cells,recovered
R15	T171	T172	nsubj	ATRX,is
R150	T313	T314	det	the,lineages
R1500	T2736	T2735	compound	ES,cells
R1501	T2737	T2733	aux	could,recovered
R1502	T2738	T2733	aux	be,recovered
R1503	T2739	T2733	cc	and,recovered
R1504	T2740	T2733	conj	maintained,recovered
R1505	T2741	T2740	prep	in,maintained
R1506	T2742	T2741	pobj	culture,in
R1507	T2743	T2733	prep	by,recovered
R1508	T2744	T2745	det	a,approach
R1509	T2745	T2743	pobj	approach,by
R151	T314	T312	pobj	lineages,of
R1510	T2746	T2747	amod	conditional,targeting
R1511	T2747	T2745	compound	targeting,approach
R1512	T2748	T2730	punct	", ",suggested
R1513	T2749	T2750	det	these,experiments
R1514	T2750	T2730	nsubj	experiments,suggested
R1515	T2751	T2750	compound	cocultivation,experiments
R1516	T2752	T2753	mark	that,impact
R1517	T2753	T2730	ccomp	impact,suggested
R1518	T2754	T2755	det	the,absence
R1519	T2755	T2753	nsubj	absence,impact
R152	T315	T314	amod	first,lineages
R1520	T2756	T2755	prep	of,absence
R1521	T2757	T2756	pobj	Atrx,of
R1522	T2758	T2753	aux	does,impact
R1523	T2759	T2753	advmod	negatively,impact
R1524	T2760	T2753	prep	upon,impact
R1525	T2761	T2762	amod	normal,growth
R1526	T2762	T2760	pobj	growth,upon
R1527	T2763	T2764	compound	ES,cell
R1528	T2764	T2762	compound	cell,growth
R1529	T2765	T2730	punct	.,suggested
R153	T316	T317	advmod	terminally,differentiated
R1530	T2767	T2768	aux	To,investigate
R1531	T2768	T2769	advcl	investigate,analysed
R1532	T2770	T2771	det	a,defect
R1533	T2771	T2768	dobj	defect,investigate
R1534	T2772	T2771	amod	possible,defect
R1535	T2773	T2774	compound	cell,cycle
R1536	T2774	T2771	compound	cycle,defect
R1537	T2775	T2774	punct	-,cycle
R1538	T2776	T2771	prep	in,defect
R1539	T2777	T2778	det	the,absence
R154	T317	T314	amod	differentiated,lineages
R1540	T2778	T2776	pobj	absence,in
R1541	T2779	T2778	prep	of,absence
R1542	T2780	T2779	pobj	Atrx,of
R1543	T2781	T2769	punct	", ",analysed
R1544	T2782	T2769	nsubj	we,analysed
R1545	T2783	T2784	det	the,distribution
R1546	T2784	T2769	dobj	distribution,analysed
R1547	T2785	T2786	compound	cell,cycle
R1548	T2786	T2784	compound	cycle,distribution
R1549	T2787	T2784	prep	of,distribution
R155	T318	T314	prep	in,lineages
R1550	T2788	T2789	npadvmod	bromodeoxyuridine,pulsed
R1551	T2789	T2794	amod	pulsed,cells
R1552	T2790	T2788	punct	(,bromodeoxyuridine
R1553	T2791	T2788	appos	BrdU,bromodeoxyuridine
R1554	T2792	T2789	punct	),pulsed
R1555	T2793	T2789	punct	-,pulsed
R1556	T2794	T2787	pobj	cells,of
R1557	T2795	T2794	compound	ES,cells
R1558	T2796	T2769	prep	by,analysed
R1559	T2797	T2798	compound	flow,cytometry
R156	T319	T320	det	the,embryo
R1560	T2798	T2796	pobj	cytometry,by
R1561	T2799	T2800	punct	(,S1A
R1562	T2800	T2769	parataxis	S1A,analysed
R1563	T2801	T2800	compound	Figure,S1A
R1564	T2802	T2800	punct	),S1A
R1565	T2803	T2769	punct	.,analysed
R1566	T2805	T2806	advmod	Surprisingly,exhibited
R1567	T2807	T2806	punct	", ",exhibited
R1568	T2808	T2809	det	both,clones
R1569	T2809	T2806	nsubj	clones,exhibited
R157	T320	T318	pobj	embryo,in
R1570	T2810	T2811	amod	Atrxnull,cell
R1571	T2811	T2809	compound	cell,clones
R1572	T2812	T2811	compound	ES,cell
R1573	T2813	T2814	det	a,profile
R1574	T2814	T2806	dobj	profile,exhibited
R1575	T2815	T2816	compound	cell,cycle
R1576	T2816	T2814	compound	cycle,profile
R1577	T2817	T2818	dep	that,was
R1578	T2818	T2814	relcl	was,profile
R1579	T2819	T2818	acomp	indistinguishable,was
R158	T321	T320	amod	developing,embryo
R1580	T2820	T2819	prep	from,indistinguishable
R1581	T2821	T2822	compound	ES,cells
R1582	T2822	T2820	pobj	cells,from
R1583	T2823	T2822	acl	bearing,cells
R1584	T2824	T2825	det	a,allele
R1585	T2825	T2823	dobj	allele,bearing
R1586	T2826	T2825	amod	functional,allele
R1587	T2827	T2825	compound	Atrx,allele
R1588	T2828	T2825	punct	(,allele
R1589	T2829	T2825	appos	Atrx WT,allele
R159	T322	T285	punct	.,implanted
R1590	T2830	T2829	cc	or,Atrx WT
R1591	T2831	T2829	conj	Atrx flox,Atrx WT
R1592	T2832	T2806	punct	),exhibited
R1593	T2833	T2806	punct	.,exhibited
R1594	T2835	T2836	nsubj	We,quantitated
R1595	T2837	T2836	advmod	also,quantitated
R1596	T2838	T2836	advmod	specifically,quantitated
R1597	T2839	T2840	det	the,index
R1598	T2840	T2836	dobj	index,quantitated
R1599	T2841	T2840	amod	mitotic,index
R16	T173	T174	det	an,member
R160	T324	T325	nmod	Carrier,mice
R1600	T2842	T2840	prep	within,index
R1601	T2843	T2844	det	each,population
R1602	T2844	T2842	pobj	population,within
R1603	T2845	T2836	prep	by,quantitated
R1604	T2846	T2847	compound	flow,cytometry
R1605	T2847	T2845	pobj	cytometry,by
R1606	T2848	T2836	prep	after,quantitated
R1607	T2849	T2848	pcomp	staining,after
R1608	T2850	T2851	compound	ES,cells
R1609	T2851	T2849	dobj	cells,staining
R161	T325	T327	nsubjpass	mice,affected
R1610	T2852	T2849	prep	for,staining
R1611	T2853	T2854	amod	phosphorylated,H3
R1612	T2854	T2852	pobj	H3,for
R1613	T2855	T2856	punct	(,Ser10
R1614	T2856	T2853	parataxis	Ser10,phosphorylated
R1615	T2857	T2856	punct	),Ser10
R1616	T2858	T2854	compound	histone,H3
R1617	T2859	T2854	punct	", ",H3
R1618	T2860	T2861	det	a,marker
R1619	T2861	T2854	appos	marker,H3
R162	T326	T325	amod	female,mice
R1620	T2862	T2861	amod	specific,marker
R1621	T2863	T2861	prep	of,marker
R1622	T2864	T2863	pobj	mitosis,of
R1623	T2865	T2866	punct	(,S1B
R1624	T2866	T2836	parataxis	S1B,quantitated
R1625	T2867	T2866	compound	Figure,S1B
R1626	T2868	T2866	punct	),S1B
R1627	T2869	T2870	punct	[,13
R1628	T2870	T2836	parataxis	13,quantitated
R1629	T2871	T2870	punct	],13
R163	T328	T329	dep	that,inherit
R1630	T2872	T2836	punct	.,quantitated
R1631	T2874	T2875	advcl	Consistent,was
R1632	T2876	T2874	prep	with,Consistent
R1633	T2877	T2878	det	the,profile
R1634	T2878	T2876	pobj	profile,with
R1635	T2879	T2878	amod	normal,profile
R1636	T2880	T2881	compound	cell,cycle
R1637	T2881	T2878	compound	cycle,profile
R1638	T2882	T2881	punct	-,cycle
R1639	T2883	T2878	acl	observed,profile
R164	T329	T325	relcl	inherit,mice
R1640	T2884	T2883	advmod	above,observed
R1641	T2885	T2875	punct	", ",was
R1642	T2886	T2875	expl	there,was
R1643	T2887	T2888	det	no,depletion
R1644	T2888	T2875	attr	depletion,was
R1645	T2889	T2888	prep	in,depletion
R1646	T2890	T2891	det	the,size
R1647	T2891	T2889	pobj	size,in
R1648	T2892	T2891	prep	of,size
R1649	T2893	T2894	det	the,population
R165	T330	T331	det	a,allele
R1650	T2894	T2892	pobj	population,of
R1651	T2895	T2894	amod	mitotic,population
R1652	T2896	T2875	prep	in,was
R1653	T2897	T2898	det	the,clones
R1654	T2898	T2896	pobj	clones,in
R1655	T2899	T2898	amod	Atrxnull,clones
R1656	T2900	T2898	compound	ES,clones
R1657	T2901	T2875	punct	", ",was
R1658	T2902	T2875	prep	despite,was
R1659	T2903	T2904	poss	their,growth
R166	T331	T329	dobj	allele,inherit
R1660	T2904	T2902	pobj	growth,despite
R1661	T2905	T2904	amod	slow,growth
R1662	T2906	T2875	punct	.,was
R1663	T2908	T2909	advmod	Finally,investigated
R1664	T2910	T2909	punct	", ",investigated
R1665	T2911	T2909	nsubj	we,investigated
R1666	T2912	T2913	mark	whether,was
R1667	T2913	T2909	ccomp	was,investigated
R1668	T2914	T2915	det	the,defect
R1669	T2915	T2913	nsubj	defect,was
R167	T332	T331	amod	maternal,allele
R1670	T2916	T2915	compound	growth,defect
R1671	T2917	T2915	prep	in,defect
R1672	T2918	T2919	det	the,cells
R1673	T2919	T2917	pobj	cells,in
R1674	T2920	T2921	amod	Atrxnull,ES
R1675	T2921	T2919	compound	ES,cells
R1676	T2922	T2913	prep	due,was
R1677	T2923	T2922	pcomp	to,due
R1678	T2924	T2925	det	an,regulation
R1679	T2925	T2922	pobj	regulation,due
R168	T333	T331	amod	null,allele
R1680	T2926	T2925	amod	up,regulation
R1681	T2927	T2925	punct	-,regulation
R1682	T2928	T2925	prep	of,regulation
R1683	T2929	T2928	pobj	apoptosis,of
R1684	T2930	T2909	prep	by,investigated
R1685	T2931	T2930	pcomp	staining,by
R1686	T2932	T2931	dobj	cells,staining
R1687	T2933	T2931	prep	with,staining
R1688	T2934	T2933	pobj	Annexin,with
R1689	T2935	T2934	nummod	V,Annexin
R169	T334	T327	aux	should,affected
R1690	T2936	T2937	punct	(,S2
R1691	T2937	T2909	parataxis	S2,investigated
R1692	T2938	T2937	compound	Figure,S2
R1693	T2939	T2937	punct	),S2
R1694	T2940	T2909	cc	and,investigated
R1695	T2941	T2909	conj	found,investigated
R1696	T2942	T2943	mark	that,affected
R1697	T2943	T2941	ccomp	affected,found
R1698	T2944	T2945	det	the,proportion
R1699	T2945	T2943	nsubjpass	proportion,affected
R17	T174	T172	attr	member,is
R170	T335	T327	auxpass	be,affected
R1700	T2946	T2945	prep	of,proportion
R1701	T2947	T2948	amod	apoptotic,cells
R1702	T2948	T2946	pobj	cells,of
R1703	T2949	T2943	auxpass	was,affected
R1704	T2950	T2943	neg	not,affected
R1705	T2951	T2943	advmod	significantly,affected
R1706	T2952	T2943	agent	by,affected
R1707	T2953	T2954	det	the,absence
R1708	T2954	T2952	pobj	absence,by
R1709	T2955	T2954	prep	of,absence
R171	T336	T327	punct	", ",affected
R1710	T2956	T2957	amod	full,length
R1711	T2957	T2959	compound	length,Atrx
R1712	T2958	T2957	punct	-,length
R1713	T2959	T2955	pobj	Atrx,of
R1714	T2960	T2909	punct	.,investigated
R1715	T2962	T2963	advmod	Thus,is
R1716	T2964	T2963	punct	", ",is
R1717	T2965	T2966	det	the,defect
R1718	T2966	T2963	nsubj	defect,is
R1719	T2967	T2966	compound	growth,defect
R172	T337	T338	mark	since,is
R1720	T2968	T2966	acl	observed,defect
R1721	T2969	T2968	prep	in,observed
R1722	T2970	T2971	compound	ES,cells
R1723	T2971	T2969	pobj	cells,in
R1724	T2972	T2971	acl	lacking,cells
R1725	T2973	T2972	dobj	Atrx,lacking
R1726	T2974	T2963	neg	not,is
R1727	T2975	T2963	prep	due,is
R1728	T2976	T2975	pcomp	to,due
R1729	T2977	T2978	det	a,block
R173	T338	T327	advcl	is,affected
R1730	T2978	T2975	pobj	block,due
R1731	T2979	T2978	amod	specific,block
R1732	T2980	T2981	compound	cell,cycle
R1733	T2981	T2978	compound	cycle,block
R1734	T2982	T2978	cc	or,block
R1735	T2983	T2984	amod	significant,induction
R1736	T2984	T2978	conj	induction,block
R1737	T2985	T2984	prep	of,induction
R1738	T2986	T2987	compound	cell,death
R1739	T2987	T2985	pobj	death,of
R174	T339	T340	det	the,chromosome
R1740	T2988	T2963	punct	.,is
R1741	T2990	T2991	mark	While,is
R1742	T2991	T2998	advcl	is,seems
R1743	T2992	T2993	det	the,cause
R1744	T2993	T2991	nsubj	cause,is
R1745	T2994	T2993	prep	of,cause
R1746	T2995	T2996	det	the,delay
R1747	T2996	T2994	pobj	delay,of
R1748	T2997	T2996	amod	proliferative,delay
R1749	T2999	T2991	neg	not,is
R175	T340	T338	nsubj	chromosome,is
R1750	T3000	T2991	advmod	yet,is
R1751	T3001	T2991	acomp	clear,is
R1752	T3002	T2991	punct	", ",is
R1753	T3003	T3004	mark	since,appear
R1754	T3004	T2991	advcl	appear,is
R1755	T3005	T3006	amod	Atrxnull,cells
R1756	T3006	T3004	nsubj	cells,appear
R1757	T3007	T3006	compound	ES,cells
R1758	T3008	T3009	aux	to,undergo
R1759	T3009	T3004	xcomp	undergo,appear
R176	T341	T340	amod	paternal,chromosome
R1760	T3010	T3011	amod	higher,rates
R1761	T3011	T3009	dobj	rates,undergo
R1762	T3012	T3011	prep	of,rates
R1763	T3013	T3014	amod	spontaneous,differentiation
R1764	T3014	T3012	pobj	differentiation,of
R1765	T3015	T3016	punct	(,data
R1766	T3016	T2991	meta	data,is
R1767	T3017	T3016	amod	unpublished,data
R1768	T3018	T3016	punct	),data
R1769	T3019	T2998	punct	", ",seems
R177	T342	T340	compound	X,chromosome
R1770	T3020	T2998	nsubj	it,seems
R1771	T3021	T2998	oprd	likely,seems
R1772	T3022	T3023	mark	that,reflects
R1773	T3023	T2998	ccomp	reflects,seems
R1774	T3024	T3025	det	the,defect
R1775	T3025	T3023	nsubj	defect,reflects
R1776	T3026	T3025	amod	observed,defect
R1777	T3027	T3025	compound	growth,defect
R1778	T3028	T3029	det	the,transition
R1779	T3029	T3023	dobj	transition,reflects
R178	T343	T338	advmod	normally,is
R1780	T3030	T3029	amod	spontaneous,transition
R1781	T3031	T3029	prep	from,transition
R1782	T3032	T3033	advmod	fast,cycling
R1783	T3033	T3035	amod	cycling,cells
R1784	T3034	T3033	punct	-,cycling
R1785	T3035	T3031	pobj	cells,from
R1786	T3036	T3035	punct	", ",cells
R1787	T3037	T3035	amod	undifferentiated,cells
R1788	T3038	T3035	compound	ES,cells
R1789	T3039	T3029	prep	into,transition
R179	T344	T338	acomp	inactivated,is
R1790	T3040	T3041	advmod	more,slowly
R1791	T3041	T3042	advmod	slowly,cycling
R1792	T3042	T3043	amod	cycling,types
R1793	T3043	T3039	pobj	types,into
R1794	T3044	T3043	punct	", ",types
R1795	T3045	T3043	amod	differentiated,types
R1796	T3046	T3043	compound	cell,types
R1797	T3047	T3023	prep	in,reflects
R1798	T3048	T3049	det	these,cultures
R1799	T3049	T3047	pobj	cultures,in
R18	T175	T174	nmod	X,member
R180	T345	T338	prep	in,is
R1800	T3050	T2998	punct	.,seems
R1801	T3052	T3053	nsubjpass	It,shown
R1802	T3054	T3053	aux	has,shown
R1803	T3055	T3053	auxpass	been,shown
R1804	T3056	T3057	mark	that,give
R1805	T3057	T3053	ccomp	give,shown
R1806	T3058	T3059	npadvmod	disease,causing
R1807	T3059	T3061	amod	causing,mutations
R1808	T3060	T3059	punct	-,causing
R1809	T3061	T3057	nsubj	mutations,give
R181	T346	T347	amod	extraembryonic,tissues
R1810	T3062	T3061	prep	in,mutations
R1811	T3063	T3064	det	the,gene
R1812	T3064	T3062	pobj	gene,in
R1813	T3065	T3064	amod	human,gene
R1814	T3066	T3064	compound	ATRX,gene
R1815	T3067	T3057	dobj	rise,give
R1816	T3068	T3057	prep	to,give
R1817	T3069	T3068	pobj	changes,to
R1818	T3070	T3069	prep	in,changes
R1819	T3071	T3072	det	the,pattern
R182	T347	T345	pobj	tissues,in
R1820	T3072	T3070	pobj	pattern,in
R1821	T3073	T3072	amod	normal,pattern
R1822	T3074	T3072	prep	of,pattern
R1823	T3075	T3076	compound	DNA,methylation
R1824	T3076	T3074	pobj	methylation,of
R1825	T3077	T3069	prep	at,changes
R1826	T3078	T3079	amod	several,sequences
R1827	T3079	T3077	pobj	sequences,at
R1828	T3080	T3079	amod	repetitive,sequences
R1829	T3081	T3079	prep	within,sequences
R183	T348	T327	punct	.,affected
R1830	T3082	T3083	det	the,genome
R1831	T3083	T3081	pobj	genome,within
R1832	T3084	T3083	amod	human,genome
R1833	T3085	T3086	punct	[,11
R1834	T3086	T3057	parataxis	11,give
R1835	T3087	T3086	punct	],11
R1836	T3088	T3053	punct	.,shown
R1837	T3090	T3091	advmod	Notably,found
R1838	T3092	T3091	punct	", ",found
R1839	T3093	T3094	det	the,region
R184	T350	T351	advmod	Surprisingly,established
R1840	T3094	T3091	nsubjpass	region,found
R1841	T3095	T3094	amod	transcribed,region
R1842	T3096	T3094	prep	of,region
R1843	T3097	T3098	det	the,repeat
R1844	T3098	T3096	pobj	repeat,of
R1845	T3099	T3100	amod	ribosomal,DNA
R1846	T3100	T3098	nmod	DNA,repeat
R1847	T3101	T3100	punct	(,DNA
R1848	T3102	T3100	appos	rDNA,DNA
R1849	T3103	T3098	punct	),repeat
R185	T352	T351	punct	", ",established
R1850	T3104	T3091	auxpass	was,found
R1851	T3105	T3106	aux	to,hypomethylated
R1852	T3106	T3091	xcomp	hypomethylated,found
R1853	T3107	T3106	auxpass	be,hypomethylated
R1854	T3108	T3106	advmod	significantly,hypomethylated
R1855	T3109	T3106	prep	in,hypomethylated
R1856	T3110	T3111	compound	ATR,X
R1857	T3111	T3113	compound	X,patients
R1858	T3112	T3111	punct	-,X
R1859	T3113	T3109	pobj	patients,in
R186	T353	T351	advmod	however,established
R1860	T3114	T3106	advcl	relative,hypomethylated
R1861	T3115	T3114	prep	to,relative
R1862	T3116	T3117	amod	normal,individuals
R1863	T3117	T3115	pobj	individuals,to
R1864	T3118	T3091	punct	.,found
R1865	T3120	T3121	advcl	Using,observed
R1866	T3122	T3123	npadvmod	methylation,sensitive
R1867	T3123	T3125	amod	sensitive,enzymes
R1868	T3124	T3123	punct	-,sensitive
R1869	T3125	T3120	dobj	enzymes,Using
R187	T354	T351	punct	", ",established
R1870	T3126	T3125	compound	restriction,enzymes
R1871	T3127	T3121	punct	", ",observed
R1872	T3128	T3121	nsubj	we,observed
R1873	T3129	T3121	advmod	also,observed
R1874	T3130	T3131	amod	significant,hypomethylation
R1875	T3131	T3121	dobj	hypomethylation,observed
R1876	T3132	T3121	prep	at,observed
R1877	T3133	T3134	amod	several,sites
R1878	T3134	T3132	pobj	sites,at
R1879	T3135	T3134	acl	tested,sites
R188	T355	T356	det	some,females
R1880	T3136	T3135	prep	within,tested
R1881	T3137	T3138	det	the,repeats
R1882	T3138	T3136	pobj	repeats,within
R1883	T3139	T3140	compound	mouse,rDNA
R1884	T3140	T3138	compound	rDNA,repeats
R1885	T3141	T3138	prep	in,repeats
R1886	T3142	T3143	amod	Atrxnull,cells
R1887	T3143	T3141	pobj	cells,in
R1888	T3144	T3143	compound	ES,cells
R1889	T3145	T3143	cc	and,cells
R189	T356	T351	nsubj	females,established
R1890	T3146	T3147	nummod	12,d
R1891	T3147	T3149	nmod	d,bodies
R1892	T3148	T3147	punct	-,d
R1893	T3149	T3143	conj	bodies,cells
R1894	T3150	T3149	amod	embryoid,bodies
R1895	T3151	T3121	advcl	relative,observed
R1896	T3152	T3151	prep	to,relative
R1897	T3153	T3154	compound	ES,cells
R1898	T3154	T3152	pobj	cells,to
R1899	T3155	T3154	cc	and,cells
R19	T176	T175	punct	-,X
R190	T357	T356	compound	carrier,females
R1900	T3156	T3157	amod	embryoid,bodies
R1901	T3157	T3154	conj	bodies,cells
R1902	T3158	T3154	acl	bearing,cells
R1903	T3159	T3160	det	a,allele
R1904	T3160	T3158	dobj	allele,bearing
R1905	T3161	T3160	amod	functional,allele
R1906	T3162	T3160	compound	Atrx,allele
R1907	T3163	T3160	punct	(,allele
R1908	T3164	T3160	appos	Atrx WT,allele
R1909	T3165	T3164	cc	or,Atrx WT
R191	T358	T359	det	a,placenta
R1910	T3166	T3164	conj	Atrx flox,Atrx WT
R1911	T3167	T3121	punct	),observed
R1912	T3168	T3169	punct	(,3B
R1913	T3169	T3121	parataxis	3B,observed
R1914	T3170	T3169	compound	Figure,3B
R1915	T3171	T3169	cc	and,3B
R1916	T3172	T3169	conj	3C,3B
R1917	T3173	T3169	punct	),3B
R1918	T3174	T3121	punct	.,observed
R1919	T3176	T3177	det	The,observation
R192	T359	T351	dobj	placenta,established
R1920	T3177	T3178	nsubj	observation,is
R1921	T3179	T3180	mark	that,hypomethylated
R1922	T3180	T3177	acl	hypomethylated,observation
R1923	T3181	T3180	nsubjpass	rDNA,hypomethylated
R1924	T3182	T3180	auxpass	is,hypomethylated
R1925	T3183	T3180	prep	in,hypomethylated
R1926	T3184	T3185	det	the,absence
R1927	T3185	T3183	pobj	absence,in
R1928	T3186	T3185	prep	of,absence
R1929	T3187	T3186	pobj	Atrx,of
R193	T360	T359	amod	normal,placenta
R1930	T3188	T3180	punct	", ",hypomethylated
R1931	T3189	T3190	advmod	even,in
R1932	T3190	T3180	prep	in,hypomethylated
R1933	T3191	T3192	compound	ES,cells
R1934	T3192	T3190	pobj	cells,in
R1935	T3193	T3178	punct	", ",is
R1936	T3194	T3178	acomp	consistent,is
R1937	T3195	T3194	prep	with,consistent
R1938	T3196	T3197	det	the,finding
R1939	T3197	T3195	pobj	finding,with
R194	T361	T351	cc	and,established
R1940	T3198	T3199	mark	that,is
R1941	T3199	T3197	acl	is,finding
R1942	T3200	T3199	nsubj	hypomethylation,is
R1943	T3201	T3200	prep	of,hypomethylation
R1944	T3202	T3203	det	the,repeats
R1945	T3203	T3201	pobj	repeats,of
R1946	T3204	T3205	amod	human,rDNA
R1947	T3205	T3203	compound	rDNA,repeats
R1948	T3206	T3199	acomp	detectable,is
R1949	T3207	T3199	prep	from,is
R195	T362	T351	conj	appeared,established
R1950	T3208	T3209	det	an,stage
R1951	T3209	T3207	pobj	stage,from
R1952	T3210	T3209	amod	early,stage
R1953	T3211	T3209	amod	developmental,stage
R1954	T3212	T3199	prep	in,is
R1955	T3213	T3214	compound	ATR,X
R1956	T3214	T3216	compound	X,patients
R1957	T3215	T3214	punct	-,X
R1958	T3216	T3212	pobj	patients,in
R1959	T3217	T3178	punct	.,is
R196	T363	T364	aux	to,escape
R1960	T3219	T3220	amod	Other,elements
R1961	T3220	T3224	nsubj	elements,include
R1962	T3221	T3220	nmod	mouse,elements
R1963	T3222	T3223	amod	repetitive,sequence
R1964	T3223	T3220	compound	sequence,elements
R1965	T3225	T3220	acl	surveyed,elements
R1966	T3226	T3225	prep	in,surveyed
R1967	T3227	T3228	compound	ES,DNA
R1968	T3228	T3226	pobj	DNA,in
R1969	T3229	T3228	compound	cell,DNA
R197	T364	T362	xcomp	escape,appeared
R1970	T3230	T3231	det	the,repeats
R1971	T3231	T3224	dobj	repeats,include
R1972	T3232	T3233	amod	heterochromatic,satellite
R1973	T3233	T3231	nmod	satellite,repeats
R1974	T3234	T3233	amod	major,satellite
R1975	T3235	T3236	punct	(,assayed
R1976	T3236	T3233	parataxis	assayed,satellite
R1977	T3237	T3236	prep	with,assayed
R1978	T3238	T3237	pobj	MaeII,with
R1979	T3239	T3236	punct	),assayed
R198	T365	T366	det	the,pattern
R1980	T3240	T3233	cc	and,satellite
R1981	T3241	T3242	amod	minor,satellite
R1982	T3242	T3233	conj	satellite,satellite
R1983	T3243	T3244	punct	(,assayed
R1984	T3244	T3242	parataxis	assayed,satellite
R1985	T3245	T3244	prep	with,assayed
R1986	T3246	T3245	pobj	HpaII,with
R1987	T3247	T3244	punct	),assayed
R1988	T3248	T3231	punct	", ",repeats
R1989	T3249	T3250	advmod	as,as
R199	T366	T364	dobj	pattern,escape
R1990	T3250	T3231	cc	as,repeats
R1991	T3251	T3250	advmod	well,as
R1992	T3252	T3253	amod	interspersed,repeats
R1993	T3253	T3231	conj	repeats,repeats
R1994	T3254	T3253	amod	retroviral,repeats
R1995	T3255	T3253	prep	of,repeats
R1996	T3256	T3257	det	the,type
R1997	T3257	T3255	pobj	type,of
R1998	T3258	T3259	amod	intracisternal,particle
R1999	T3259	T3257	nmod	particle,type
R2	T155	T152	prep	of,Loss
R20	T177	T175	amod	encoded,X
R200	T367	T366	amod	usual,pattern
R2000	T3260	T3259	nmod	A,particle
R2001	T3261	T3259	punct	(,particle
R2002	T3262	T3259	appos	IAP,particle
R2003	T3263	T3257	punct	),type
R2004	T3264	T3257	cc	and,type
R2005	T3265	T3266	det	the,families
R2006	T3266	T3257	conj	families,type
R2007	T3267	T3266	nmod	Line,families
R2008	T3268	T3267	nummod	1,Line
R2009	T3269	T3267	cc	and,Line
R201	T368	T366	prep	of,pattern
R2010	T3270	T3271	compound	Sine,B1
R2011	T3271	T3267	conj	B1,Line
R2012	T3272	T3273	punct	(,all
R2013	T3273	T3266	parataxis	all,families
R2014	T3274	T3273	acl	assayed,all
R2015	T3275	T3274	prep	with,assayed
R2016	T3276	T3275	pobj	HpaII,with
R2017	T3277	T3273	punct	),all
R2018	T3278	T3224	punct	.,include
R2019	T3280	T3281	det	These,repeats
R202	T369	T370	amod	imprinted,inactivation
R2020	T3281	T3282	nsubjpass	repeats,found
R2021	T3283	T3282	auxpass	were,found
R2022	T3284	T3285	aux	to,be
R2023	T3285	T3282	xcomp	be,found
R2024	T3286	T3287	advmod	moderately,methylated
R2025	T3287	T3285	acomp	methylated,be
R2026	T3288	T3289	punct	(,Line
R2027	T3289	T3286	parataxis	Line,moderately
R2028	T3290	T3289	nummod	1,Line
R2029	T3291	T3289	cc	and,Line
R203	T370	T368	pobj	inactivation,of
R2030	T3292	T3293	compound	Sine,B1
R2031	T3293	T3289	conj	B1,Line
R2032	T3294	T3289	punct	),Line
R2033	T3295	T3286	cc	or,moderately
R2034	T3296	T3286	conj	highly,moderately
R2035	T3297	T3298	punct	(,IAP
R2036	T3298	T3296	parataxis	IAP,highly
R2037	T3299	T3298	punct	", ",IAP
R2038	T3300	T3301	amod	major,satellite
R2039	T3301	T3298	appos	satellite,IAP
R204	T371	T370	compound	X,inactivation
R2040	T3302	T3298	punct	", ",IAP
R2041	T3303	T3304	amod	minor,satellite
R2042	T3304	T3298	appos	satellite,IAP
R2043	T3305	T3298	punct	),IAP
R2044	T3306	T3285	prep	in,be
R2045	T3307	T3308	amod	wild,type
R2046	T3308	T3310	compound	type,cells
R2047	T3309	T3308	punct	-,type
R2048	T3310	T3306	pobj	cells,in
R2049	T3311	T3310	compound	ES,cells
R205	T372	T370	punct	-,inactivation
R2050	T3312	T3282	punct	", ",found
R2051	T3313	T3282	cc	and,found
R2052	T3314	T3315	det	this,methylation
R2053	T3315	T3316	nsubjpass	methylation,perturbed
R2054	T3316	T3282	conj	perturbed,found
R2055	T3317	T3316	auxpass	was,perturbed
R2056	T3318	T3316	neg	not,perturbed
R2057	T3319	T3316	advmod	detectably,perturbed
R2058	T3320	T3316	agent	by,perturbed
R2059	T3321	T3322	det	the,absence
R206	T373	T364	prep	in,escape
R2060	T3322	T3320	pobj	absence,by
R2061	T3323	T3322	prep	of,absence
R2062	T3324	T3323	pobj	Atrx,of
R2063	T3325	T3326	punct	(,S3
R2064	T3326	T3316	parataxis	S3,perturbed
R2065	T3327	T3326	compound	Figure,S3
R2066	T3328	T3326	cc	and,S3
R2067	T3329	T3330	amod	unpublished,data
R2068	T3330	T3326	conj	data,S3
R2069	T3331	T3326	punct	),S3
R207	T374	T375	det	these,tissues
R2070	T3332	T3316	punct	.,perturbed
R2071	T3334	T3335	advcl	Taken,indicate
R2072	T3336	T3334	advmod	together,Taken
R2073	T3337	T3335	punct	", ",indicate
R2074	T3338	T3339	det	these,data
R2075	T3339	T3335	nsubj	data,indicate
R2076	T3340	T3341	mark	that,is
R2077	T3341	T3335	ccomp	is,indicate
R2078	T3342	T3343	det	the,interplay
R2079	T3343	T3341	nsubj	interplay,is
R208	T375	T373	pobj	tissues,in
R2080	T3344	T3343	amod	subtle,interplay
R2081	T3345	T3343	prep	between,interplay
R2082	T3346	T3347	det	the,protein
R2083	T3347	T3345	pobj	protein,between
R2084	T3348	T3347	compound	ATRX,protein
R2085	T3349	T3347	cc	and,protein
R2086	T3350	T3351	compound	DNA,methylation
R2087	T3351	T3347	conj	methylation,protein
R2088	T3352	T3343	acl	observed,interplay
R2089	T3353	T3352	prep	in,observed
R209	T376	T351	punct	.,established
R2090	T3354	T3355	amod	human,patients
R2091	T3355	T3353	pobj	patients,in
R2092	T3356	T3341	advmod	also,is
R2093	T3357	T3341	acomp	present,is
R2094	T3358	T3341	prep	in,is
R2095	T3359	T3360	compound	mouse,cells
R2096	T3360	T3358	pobj	cells,in
R2097	T3361	T3335	punct	.,indicate
R21	T178	T174	prep	of,member
R210	T378	T379	advmod	Together,demonstrate
R2105	T3956	T3957	amod	Early,Lethality
R2106	T3958	T3957	amod	Embryonic,Lethality
R2107	T3959	T3957	prep	in,Lethality
R2108	T3960	T3961	amod	Atrxnull,Mice
R2109	T3961	T3959	pobj	Mice,in
R211	T380	T381	det	these,findings
R2110	T3962	T3961	amod	Male,Mice
R2111	T3964	T3965	aux	To,investigate
R2112	T3965	T3966	advcl	investigate,established
R2113	T3967	T3968	det	the,role
R2114	T3968	T3965	dobj	role,investigate
R2115	T3969	T3968	prep	of,role
R2116	T3970	T3971	det	the,protein
R2117	T3971	T3969	pobj	protein,of
R2118	T3972	T3971	compound	Atrx,protein
R2119	T3973	T3968	prep	during,role
R212	T381	T379	nsubj	findings,demonstrate
R2120	T3974	T3975	compound	mouse,development
R2121	T3975	T3973	pobj	development,during
R2122	T3976	T3966	punct	", ",established
R2123	T3977	T3966	nsubj	we,established
R2124	T3978	T3966	advmod	initially,established
R2125	T3979	T3966	dobj	lines,established
R2126	T3980	T3979	prep	of,lines
R2127	T3981	T3980	pobj	mice,of
R2128	T3982	T3979	acl	bearing,lines
R2129	T3983	T3984	det	the,allele
R213	T382	T383	det	an,role
R2130	T3984	T3982	dobj	allele,bearing
R2131	T3985	T3984	compound	Atrx flox,allele
R2132	T3986	T3966	punct	.,established
R2133	T3988	T3989	nummod	Two,clones
R2134	T3989	T3995	nsubjpass	clones,injected
R2135	T3990	T3991	advmod	independently,targeted
R2136	T3991	T3989	amod	targeted,clones
R2137	T3992	T3989	compound	Atrx flox,clones
R2138	T3993	T3994	compound	ES,cell
R2139	T3994	T3989	compound	cell,clones
R214	T383	T379	dobj	role,demonstrate
R2140	T3996	T3989	prep	with,clones
R2141	T3997	T3998	amod	normal,karyotype
R2142	T3998	T3996	pobj	karyotype,with
R2143	T3999	T3998	amod	male,karyotype
R2144	T4000	T3995	auxpass	were,injected
R2145	T4001	T3995	prep	into,injected
R2146	T4002	T4003	nmod	C57BL,blastocysts
R2147	T4003	T4001	pobj	blastocysts,into
R2148	T4004	T4002	punct	/,C57BL
R2149	T4005	T4002	nummod	6,C57BL
R215	T384	T383	amod	unexpected,role
R2150	T4006	T4007	aux	to,produce
R2151	T4007	T3995	advcl	produce,injected
R2152	T4008	T4009	amod	chimaeric,mice
R2153	T4009	T4007	dobj	mice,produce
R2154	T4010	T4009	punct	", ",mice
R2155	T4011	T4012	dep	which,used
R2156	T4012	T4009	relcl	used,mice
R2157	T4013	T4012	auxpass	were,used
R2158	T4014	T4012	advmod	then,used
R2159	T4015	T4016	aux	to,obtain
R216	T385	T384	punct	", ",unexpected
R2160	T4016	T4012	advcl	obtain,used
R2161	T4017	T4018	compound	germline,transmission
R2162	T4018	T4016	dobj	transmission,obtain
R2163	T4019	T3995	punct	.,injected
R2164	T4021	T4022	nsubjpass	Intercrosses,carried
R2165	T4023	T4021	prep	between,Intercrosses
R2166	T4024	T4023	pobj	males,between
R2167	T4025	T4024	amod	hemizygous,males
R2168	T4026	T4027	punct	(,Y
R2169	T4027	T4025	parataxis	Y,hemizygous
R217	T386	T384	conj	specific,unexpected
R2170	T4028	T4027	compound	Atrx flox,Y
R2171	T4029	T4027	punct	/,Y
R2172	T4030	T4027	punct	),Y
R2173	T4031	T4024	cc	and,males
R2174	T4032	T4024	conj	females,males
R2175	T4033	T4032	amod	heterozygous,females
R2176	T4034	T4035	punct	(,flox
R2177	T4035	T4033	parataxis	flox,heterozygous
R2178	T4036	T4035	compound	Atrx WT,flox
R2179	T4037	T4035	punct	/,flox
R218	T387	T386	punct	", ",specific
R2180	T4038	T4035	punct	),flox
R2181	T4039	T4032	prep	for,females
R2182	T4040	T4041	det	the,allele
R2183	T4041	T4039	pobj	allele,for
R2184	T4042	T4041	amod	floxed,allele
R2185	T4043	T4022	auxpass	were,carried
R2186	T4044	T4022	advmod	also,carried
R2187	T4045	T4022	prt	out,carried
R2188	T4046	T4047	aux	to,generate
R2189	T4047	T4022	advcl	generate,carried
R219	T388	T386	cc	and,specific
R2190	T4048	T4049	amod	homozygous,females
R2191	T4049	T4047	dobj	females,generate
R2192	T4050	T4051	punct	(,flox
R2193	T4051	T4049	parataxis	flox,females
R2194	T4052	T4051	compound	Atrx flox,flox
R2195	T4053	T4051	punct	/,flox
R2196	T4054	T4051	punct	),flox
R2197	T4055	T4022	punct	.,carried
R2198	T4057	T4058	nsubj	Males,were
R2199	T4059	T4057	amod	hemizygous,Males
R22	T179	T180	det	the,family
R220	T389	T386	conj	essential,specific
R2200	T4060	T4057	cc	and,Males
R2201	T4061	T4057	conj	females,Males
R2202	T4062	T4061	amod	heterozygous,females
R2203	T4063	T4062	cc	or,heterozygous
R2204	T4064	T4062	conj	homozygous,heterozygous
R2205	T4065	T4061	prep	for,females
R2206	T4066	T4067	det	the,allele
R2207	T4067	T4065	pobj	allele,for
R2208	T4068	T4067	compound	Atrx flox,allele
R2209	T4069	T4058	acomp	viable,were
R221	T390	T383	prep	for,role
R2210	T4070	T4058	punct	", ",were
R2211	T4071	T4058	conj	appeared,were
R2212	T4072	T4071	oprd	healthy,appeared
R2213	T4073	T4071	punct	", ",appeared
R2214	T4074	T4071	cc	and,appeared
R2215	T4075	T4071	conj	bred,appeared
R2216	T4076	T4075	advmod	normally,bred
R2217	T4077	T4058	punct	", ",were
R2218	T4078	T4058	advcl	suggesting,were
R2219	T4079	T4080	mark	that,was
R222	T391	T390	pobj	Atrx,for
R2220	T4080	T4078	ccomp	was,suggesting
R2221	T4081	T4080	punct	", ",was
R2222	T4082	T4083	mark	as,expected
R2223	T4083	T4080	advcl	expected,was
R2224	T4084	T4080	punct	", ",was
R2225	T4085	T4086	det	the,allele
R2226	T4086	T4080	nsubj	allele,was
R2227	T4087	T4086	compound	Atrx flox,allele
R2228	T4088	T4089	advmod	functionally,normal
R2229	T4089	T4080	acomp	normal,was
R223	T392	T383	prep	in,role
R2230	T4090	T4058	punct	.,were
R2231	T4092	T4093	aux	To,generate
R2232	T4093	T4094	advcl	generate,crossed
R2233	T4095	T4096	amod	Atrxnull,mice
R2234	T4096	T4093	dobj	mice,generate
R2235	T4097	T4093	prep	by,generate
R2236	T4098	T4099	npadvmod	Cre,mediated
R2237	T4099	T4101	amod	mediated,recombination
R2238	T4100	T4099	punct	-,mediated
R2239	T4101	T4097	pobj	recombination,by
R224	T393	T394	det	the,development
R2240	T4102	T4094	punct	", ",crossed
R2241	T4103	T4104	det	the,mice
R2242	T4104	T4094	nsubjpass	mice,crossed
R2243	T4105	T4104	compound	Atrx flox,mice
R2244	T4106	T4094	auxpass	were,crossed
R2245	T4107	T4094	prep	with,crossed
R2246	T4108	T4107	pobj	mice,with
R2247	T4109	T4108	acl	harboring,mice
R2248	T4110	T4111	det	a,transgene
R2249	T4111	T4109	dobj	transgene,harboring
R225	T394	T392	pobj	development,in
R2250	T4112	T4113	prep	in,expressed
R2251	T4113	T4111	relcl	expressed,transgene
R2252	T4114	T4112	pobj	which,in
R2253	T4115	T4116	det	the,recombinase
R2254	T4116	T4113	nsubjpass	recombinase,expressed
R2255	T4117	T4116	compound	Cre,recombinase
R2256	T4118	T4113	auxpass	is,expressed
R2257	T4119	T4113	prep	under,expressed
R2258	T4120	T4121	det	the,control
R2259	T4121	T4119	pobj	control,under
R226	T395	T394	prep	of,development
R2260	T4122	T4121	prep	of,control
R2261	T4123	T4124	det	the,elements
R2262	T4124	T4122	pobj	elements,of
R2263	T4125	T4124	amod	regulatory,elements
R2264	T4126	T4124	prep	of,elements
R2265	T4127	T4128	det	the,gene
R2266	T4128	T4126	pobj	gene,of
R2267	T4129	T4128	nmod	mouse,gene
R2268	T4130	T4128	nmod	GATA,gene
R2269	T4131	T4130	punct	-,GATA
R227	T396	T397	det	the,trophoblast
R2270	T4132	T4130	nummod	1,GATA
R2271	T4133	T4128	punct	(,gene
R2272	T4134	T4135	compound	GATA1,cre
R2273	T4135	T4128	appos	cre,gene
R2274	T4136	T4135	punct	-,cre
R2275	T4137	T4094	punct	),crossed
R2276	T4138	T4139	punct	[,14
R2277	T4139	T4094	parataxis	14,crossed
R2278	T4140	T4139	punct	],14
R2279	T4141	T4094	punct	.,crossed
R228	T397	T395	pobj	trophoblast,of
R2280	T4143	T4144	amod	Widespread,expression
R2281	T4144	T4145	nsubjpass	expression,demonstrated
R2282	T4146	T4144	prep	of,expression
R2283	T4147	T4148	det	the,transgene
R2284	T4148	T4146	pobj	transgene,of
R2285	T4149	T4150	compound	GATA1,cre
R2286	T4150	T4148	compound	cre,transgene
R2287	T4151	T4150	punct	-,cre
R2288	T4152	T4145	aux	has,demonstrated
R2289	T4153	T4145	auxpass	been,demonstrated
R229	T398	T397	amod	murine,trophoblast
R2290	T4154	T4145	prep	during,demonstrated
R2291	T4155	T4156	amod	early,embryogenesis
R2292	T4156	T4154	pobj	embryogenesis,during
R2293	T4157	T4158	punct	[,14
R2294	T4158	T4145	parataxis	14,demonstrated
R2295	T4159	T4158	punct	],14
R2296	T4160	T4145	punct	.,demonstrated
R2297	T4162	T4163	nsubj	We,defined
R2298	T4164	T4165	advmod	more,accurately
R2299	T4165	T4163	advmod	accurately,defined
R23	T180	T178	pobj	family,of
R230	T399	T379	cc	and,demonstrate
R2300	T4166	T4167	det	the,onset
R2301	T4167	T4163	dobj	onset,defined
R2302	T4168	T4167	prep	of,onset
R2303	T4169	T4170	compound	GATA1,cre
R2304	T4170	T4172	compound	cre,expression
R2305	T4171	T4170	punct	-,cre
R2306	T4172	T4168	pobj	expression,of
R2307	T4173	T4163	advcl	using,defined
R2308	T4174	T4175	det	a,strain
R2309	T4175	T4173	dobj	strain,using
R231	T400	T379	conj	present,demonstrate
R2310	T4176	T4175	compound	ROSA26,strain
R2311	T4177	T4175	compound	reporter,strain
R2312	T4178	T4175	punct	", ",strain
R2313	T4179	T4180	prep	in,expressed
R2314	T4180	T4175	relcl	expressed,strain
R2315	T4181	T4179	pobj	which,in
R2316	T4182	T4183	det	a,gene
R2317	T4183	T4180	nsubjpass	gene,expressed
R2318	T4184	T4185	nmod	β,galactosidase
R2319	T4185	T4187	nmod	galactosidase,fusion
R232	T401	T402	det	an,example
R2320	T4186	T4185	punct	-,galactosidase
R2321	T4187	T4183	compound	fusion,gene
R2322	T4188	T4185	punct	/,galactosidase
R2323	T4189	T4185	appos	neor,galactosidase
R2324	T4190	T4183	compound	reporter,gene
R2325	T4191	T4180	auxpass	is,expressed
R2326	T4192	T4193	advmod	only,after
R2327	T4193	T4180	prep	after,expressed
R2328	T4194	T4195	npadvmod	Cre,mediated
R2329	T4195	T4197	amod	mediated,excision
R233	T402	T400	dobj	example,present
R2330	T4196	T4195	punct	-,mediated
R2331	T4197	T4193	pobj	excision,after
R2332	T4198	T4197	prep	of,excision
R2333	T4199	T4200	npadvmod	loxP,flanked
R2334	T4200	T4202	amod	flanked,transcription
R2335	T4201	T4200	punct	-,flanked
R2336	T4202	T4198	pobj	transcription,of
R2337	T4203	T4197	cc	and,excision
R2338	T4204	T4205	compound	translation,termination
R2339	T4205	T4206	compound	termination,signals
R234	T403	T402	prep	of,example
R2340	T4206	T4197	conj	signals,excision
R2341	T4207	T4208	punct	[,14
R2342	T4208	T4163	parataxis	14,defined
R2343	T4209	T4208	punct	],14
R2344	T4210	T4163	punct	.,defined
R2345	T4212	T4213	nsubj	We,found
R2346	T4214	T4215	mark	that,was
R2347	T4215	T4213	ccomp	was,found
R2348	T4216	T4217	det	the,transgene
R2349	T4217	T4215	nsubj	transgene,was
R235	T404	T403	pobj	escape,of
R2350	T4218	T4219	compound	GATA1,cre
R2351	T4219	T4217	compound	cre,transgene
R2352	T4220	T4219	punct	-,cre
R2353	T4221	T4215	advmod	already,was
R2354	T4222	T4215	acomp	active,was
R2355	T4223	T4215	prep	at,was
R2356	T4224	T4225	det	the,stage
R2357	T4225	T4223	pobj	stage,at
R2358	T4226	T4227	nummod	16,cell
R2359	T4227	T4225	compound	cell,stage
R236	T405	T404	prep	from,escape
R2360	T4228	T4227	punct	-,cell
R2361	T4229	T4225	compound	morula,stage
R2362	T4230	T4225	prep	of,stage
R2363	T4231	T4230	pobj	development,of
R2364	T4232	T4225	punct	(,stage
R2365	T4233	T4234	nummod	0.5,days
R2366	T4234	T4235	npadvmod	days,postcoitus
R2367	T4235	T4225	advmod	postcoitus,stage
R2368	T4236	T4237	punct	[,dpc
R2369	T4237	T4235	parataxis	dpc,postcoitus
R237	T406	T407	amod	imprinted,chromosome
R2370	T4238	T4237	punct	],dpc
R2371	T4239	T4213	punct	),found
R2372	T4240	T4241	punct	(,4A
R2373	T4241	T4213	parataxis	4A,found
R2374	T4242	T4241	compound	Figure,4A
R2375	T4243	T4241	punct	),4A
R2376	T4244	T4213	punct	.,found
R2377	T4246	T4247	aux	To,generate
R2378	T4247	T4248	advcl	generate,mated
R2379	T4249	T4250	amod	Atrxnull,mice
R238	T407	T409	compound	chromosome,inactivation
R2380	T4250	T4247	dobj	mice,generate
R2381	T4251	T4248	punct	", ",mated
R2382	T4252	T4253	amod	heterozygous,females
R2383	T4253	T4248	nsubjpass	females,mated
R2384	T4254	T4253	amod	floxed,females
R2385	T4255	T4256	punct	(,flox
R2386	T4256	T4253	parataxis	flox,females
R2387	T4257	T4256	compound	Atrx WT,flox
R2388	T4258	T4256	punct	/,flox
R2389	T4259	T4256	punct	),flox
R239	T408	T407	compound	X,chromosome
R2390	T4260	T4248	auxpass	were,mated
R2391	T4261	T4248	prep	with,mated
R2392	T4262	T4263	amod	homozygous,males
R2393	T4263	T4261	pobj	males,with
R2394	T4264	T4265	compound	GATA1,cre
R2395	T4265	T4267	npadvmod	cre,transgenic
R2396	T4266	T4265	punct	-,cre
R2397	T4267	T4263	amod	transgenic,males
R2398	T4268	T4269	punct	(,cre
R2399	T4269	T4263	parataxis	cre,males
R24	T181	T180	compound	SNF2,family
R240	T409	T405	pobj	inactivation,from
R2400	T4270	T4271	compound	Atrx WT,Y
R2401	T4271	T4269	dep	Y,cre
R2402	T4272	T4271	punct	/,Y
R2403	T4273	T4269	punct	;,cre
R2404	T4274	T4269	compound	GATA1,cre
R2405	T4275	T4269	punct	-,cre
R2406	T4276	T4269	punct	+,cre
R2407	T4277	T4269	punct	/,cre
R2408	T4278	T4269	punct	+,cre
R2409	T4279	T4269	punct	),cre
R241	T410	T379	punct	.,demonstrate
R2410	T4280	T4248	punct	.,mated
R2411	T4282	T4283	det	No,males
R2412	T4283	T4285	nsubjpass	males,recovered
R2413	T4284	T4283	amod	Atrxnull,males
R2414	T4286	T4287	punct	(,cre
R2415	T4287	T4283	parataxis	cre,males
R2416	T4288	T4289	compound	Atrx null,Y
R2417	T4289	T4287	dep	Y,cre
R2418	T4290	T4289	punct	/,Y
R2419	T4291	T4287	punct	;,cre
R242	T160	T161	det	the,Pattern
R2420	T4292	T4287	compound	GATA1,cre
R2421	T4293	T4287	punct	-,cre
R2422	T4294	T4287	punct	+,cre
R2423	T4295	T4287	punct	/,cre
R2424	T4296	T4287	punct	−,cre
R2425	T4297	T4287	punct	),cre
R2426	T4298	T4285	auxpass	were,recovered
R2427	T4299	T4285	prep	at,recovered
R2428	T4300	T4299	pobj	birth,at
R2429	T4301	T4285	punct	", ",recovered
R2430	T4302	T4285	advcl	indicating,recovered
R2431	T4303	T4304	mark	that,results
R2432	T4304	T4302	ccomp	results,indicating
R2433	T4305	T4306	det	the,absence
R2434	T4306	T4304	nsubj	absence,results
R2435	T4307	T4306	prep	of,absence
R2436	T4308	T4307	pobj	Atrx,of
R2437	T4309	T4304	prep	in,results
R2438	T4310	T4311	amod	embryonic,lethality
R2439	T4311	T4309	pobj	lethality,in
R2440	T4312	T4285	punct	.,recovered
R2441	T4314	T4315	det	This,finding
R2442	T4315	T4316	nsubj	finding,was
R2443	T4317	T4316	acomp	unexpected,was
R2444	T4318	T4316	punct	", ",was
R2445	T4319	T4320	mark	since,survive
R2446	T4320	T4316	advcl	survive,was
R2447	T4321	T4322	amod	human,patients
R2448	T4322	T4320	nsubj	patients,survive
R2449	T4323	T4324	compound	ATR,X
R2450	T4324	T4322	compound	X,patients
R2451	T4325	T4324	punct	-,X
R2452	T4326	T4320	advmod	clearly,survive
R2453	T4327	T4320	prep	to,survive
R2454	T4328	T4327	pobj	adulthood,to
R2455	T4329	T4330	punct	(,see
R2456	T4330	T4320	parataxis	see,survive
R2457	T4331	T4330	dobj	Discussion,see
R2458	T4332	T4330	punct	),see
R2459	T4333	T4316	punct	.,was
R2460	T4335	T4336	nsubjpass	Embryos,dissected
R2461	T4337	T4336	auxpass	were,dissected
R2462	T4338	T4336	prep	at,dissected
R2463	T4339	T4340	nummod	7.5,dpc
R2464	T4340	T4338	pobj	dpc,at
R2465	T4341	T4339	punct	", ",7.5
R2466	T4342	T4339	conj	8.5,7.5
R2467	T4343	T4342	punct	", ",8.5
R2468	T4344	T4342	cc	and,8.5
R2469	T4345	T4342	conj	9.5,8.5
R2470	T4346	T4336	cc	and,dissected
R2471	T4347	T4336	conj	genotyped,dissected
R2472	T4348	T4347	prep	by,genotyped
R2473	T4349	T4350	compound	PCR,analysis
R2474	T4350	T4348	pobj	analysis,by
R2475	T4351	T4350	prep	of,analysis
R2476	T4352	T4351	pobj	DNA,of
R2477	T4353	T4352	acl	extracted,DNA
R2478	T4354	T4353	prep	from,extracted
R2479	T4355	T4356	compound	yolk,sac
R2480	T4356	T4354	pobj	sac,from
R2481	T4357	T4356	cc	or,sac
R2482	T4358	T4359	amod	total,embryo
R2483	T4359	T4356	conj	embryo,sac
R2484	T4360	T4361	punct	(,4B
R2485	T4361	T4336	parataxis	4B,dissected
R2486	T4362	T4361	compound	Figure,4B
R2487	T4363	T4361	cc	and,4B
R2488	T4364	T4365	compound	Protocol,S1
R2489	T4365	T4361	conj	S1,4B
R249	T722	T723	compound	ATR,X
R2490	T4366	T4361	punct	),4B
R2491	T4367	T4336	punct	.,dissected
R2492	T4369	T4370	amod	Atrxnull,males
R2493	T4370	T4371	nsubj	males,were
R2494	T4372	T4371	acomp	present,were
R2495	T4373	T4371	prep	at,were
R2496	T4374	T4375	amod	expected,ratios
R2497	T4375	T4373	pobj	ratios,at
R2498	T4376	T4375	amod	mendelian,ratios
R2499	T4377	T4378	punct	(,%
R25	T182	T180	prep	of,family
R250	T723	T725	compound	X,syndrome
R2500	T4378	T4375	parataxis	%,ratios
R2501	T4379	T4380	punct	~,25
R2502	T4380	T4378	nummod	25,%
R2503	T4381	T4378	punct	),%
R2504	T4382	T4371	prep	at,were
R2505	T4383	T4384	preconj	both,dpc
R2506	T4384	T4382	pobj	dpc,at
R2507	T4385	T4384	nummod	7.5,dpc
R2508	T4386	T4384	cc	and,dpc
R2509	T4387	T4388	nummod	8.5,dpc
R251	T724	T723	punct	-,X
R2510	T4388	T4384	conj	dpc,dpc
R2511	T4389	T4390	punct	(,Table
R2512	T4390	T4371	parataxis	Table,were
R2513	T4391	T4390	nummod	1,Table
R2514	T4392	T4390	punct	),Table
R2515	T4393	T4371	punct	.,were
R2516	T4395	T4396	advmod	However,observed
R2517	T4397	T4396	punct	", ",observed
R2518	T4398	T4396	prep	by,observed
R2519	T4399	T4400	nummod	9.5,dpc
R252	T725	T726	nsubj	syndrome,is
R2520	T4400	T4398	pobj	dpc,by
R2521	T4401	T4396	punct	", ",observed
R2522	T4402	T4396	nsubjpass	depletion,observed
R2523	T4403	T4396	auxpass	was,observed
R2524	T4404	T4405	preconj	both,in
R2525	T4405	T4396	prep	in,observed
R2526	T4406	T4407	det	the,number
R2527	T4407	T4405	pobj	number,in
R2528	T4408	T4407	prep	of,number
R2529	T4409	T4410	amod	Atrxnull,males
R253	T727	T728	det	a,form
R2530	T4410	T4408	pobj	males,of
R2531	T4411	T4412	punct	(,%
R2532	T4412	T4407	parataxis	%,number
R2533	T4413	T4412	nummod	7,%
R2534	T4414	T4412	punct	),%
R2535	T4415	T4405	cc	and,in
R2536	T4416	T4405	conj	in,in
R2537	T4417	T4418	det	the,number
R2538	T4418	T4416	pobj	number,in
R2539	T4419	T4418	amod	total,number
R254	T728	T726	attr	form,is
R2540	T4420	T4418	prep	of,number
R2541	T4421	T4420	pobj	males,of
R2542	T4422	T4421	acl	recovered,males
R2543	T4423	T4424	punct	(,%
R2544	T4424	T4418	parataxis	%,number
R2545	T4425	T4424	nummod	31,%
R2546	T4426	T4424	punct	),%
R2547	T4427	T4396	punct	.,observed
R2548	T4429	T4430	det	No,males
R2549	T4430	T4432	nsubjpass	males,recovered
R255	T729	T728	amod	severe,form
R2550	T4431	T4430	amod	Atrxnull,males
R2551	T4433	T4432	auxpass	were,recovered
R2552	T4434	T4432	prep	after,recovered
R2553	T4435	T4436	nummod	9.5,dpc
R2554	T4436	T4434	pobj	dpc,after
R2555	T4437	T4432	punct	.,recovered
R2556	T4439	T4440	advmod	Thus,gives
R2557	T4441	T4442	det	the,absence
R2558	T4442	T4440	nsubj	absence,gives
R2559	T4443	T4442	prep	of,absence
R256	T730	T728	punct	", ",form
R2560	T4444	T4443	pobj	Atrx,of
R2561	T4445	T4440	dobj	rise,gives
R2562	T4446	T4440	prep	to,gives
R2563	T4447	T4448	amod	embryonic,lethality
R2564	T4448	T4446	pobj	lethality,to
R2565	T4449	T4440	prep	in,gives
R2566	T4450	T4449	pobj	mice,in
R2567	T4451	T4440	prep	before,gives
R2568	T4452	T4453	nummod	9.5,dpc
R2569	T4453	T4451	pobj	dpc,before
R257	T731	T728	amod	nonprogressive,form
R2570	T4454	T4440	punct	.,gives
R2571	T4456	T4457	aux	Table 1 Distribution of Atrx Genotypes in Timed Matings To,investigate
R2572	T4457	T4458	advcl	investigate,dissected
R2573	T4459	T4460	det	the,morphology
R2574	T4460	T4457	dobj	morphology,investigate
R2575	T4461	T4460	prep	of,morphology
R2576	T4462	T4463	amod	Atrxnull,embryos
R2577	T4463	T4461	pobj	embryos,of
R2578	T4464	T4465	advmod	prior,to
R2579	T4465	T4460	prep	to,morphology
R258	T732	T728	prep	of,form
R2580	T4466	T4465	pobj	death,to
R2581	T4467	T4458	punct	", ",dissected
R2582	T4468	T4458	nsubjpass	embryos,dissected
R2583	T4469	T4468	prep	from,embryos
R2584	T4470	T4471	det	the,crosses
R2585	T4471	T4469	pobj	crosses,from
R2586	T4472	T4471	amod	above,crosses
R2587	T4473	T4458	auxpass	were,dissected
R2588	T4474	T4458	advmod	initially,dissected
R2589	T4475	T4458	prep	in,dissected
R259	T733	T734	npadvmod	X,linked
R2590	T4476	T4477	poss	their,deciduas
R2591	T4477	T4475	pobj	deciduas,in
R2592	T4478	T4458	prep	at,dissected
R2593	T4479	T4480	nummod	7.5,dpc
R2594	T4480	T4478	pobj	dpc,at
R2595	T4481	T4458	punct	", ",dissected
R2596	T4482	T4458	cc	and,dissected
R2597	T4483	T4484	compound	paraffin,sections
R2598	T4484	T4485	nsubjpass	sections,stained
R2599	T4485	T4458	conj	stained,dissected
R26	T183	T184	compound	ATPase,helicase
R260	T734	T736	amod	linked,retardation
R2600	T4486	T4485	auxpass	were,stained
R2601	T4487	T4485	prep	with,stained
R2602	T4488	T4487	pobj	haematoxylin,with
R2603	T4489	T4490	punct	(,5A
R2604	T4490	T4487	parataxis	5A,with
R2605	T4491	T4490	compound	Figure,5A
R2606	T4492	T4490	punct	),5A
R2607	T4493	T4487	cc	or,with
R2608	T4494	T4487	conj	with,with
R2609	T4495	T4496	det	an,antibody
R261	T735	T734	punct	-,linked
R2610	T4496	T4494	pobj	antibody,with
R2611	T4497	T4496	amod	anti-ATRX,antibody
R2612	T4498	T4499	punct	(,5B
R2613	T4499	T4458	parataxis	5B,dissected
R2614	T4500	T4499	compound	Figure,5B
R2615	T4501	T4502	punct	–,5E
R2616	T4502	T4499	prep	5E,5B
R2617	T4503	T4499	punct	),5B
R2618	T4504	T4458	punct	.,dissected
R2619	T4506	T4507	amod	Immunohistochemical,staining
R262	T736	T732	pobj	retardation,of
R2620	T4507	T4508	nsubj	staining,revealed
R2621	T4509	T4510	mark	that,expressed
R2622	T4510	T4508	ccomp	expressed,revealed
R2623	T4511	T4510	nsubjpass	Atrx,expressed
R2624	T4512	T4510	auxpass	was,expressed
R2625	T4513	T4510	advmod	widely,expressed
R2626	T4514	T4510	prep	in,expressed
R2627	T4515	T4516	amod	wild,type
R2628	T4516	T4518	nmod	type,embryos
R2629	T4517	T4516	punct	-,type
R263	T737	T736	amod	mental,retardation
R2630	T4518	T4514	pobj	embryos,in
R2631	T4519	T4520	nummod	7.5,dpc
R2632	T4520	T4518	compound	dpc,embryos
R2633	T4521	T4522	punct	(,5B
R2634	T4522	T4508	parataxis	5B,revealed
R2635	T4523	T4522	compound	Figure,5B
R2636	T4524	T4522	punct	),5B
R2637	T4525	T4508	punct	.,revealed
R2638	T4527	T4528	nsubj	Expression,was
R2639	T4529	T4528	acomp	highest,was
R264	T738	T739	dep	that,associated
R2640	T4530	T4528	prep	in,was
R2641	T4531	T4532	det	the,region
R2642	T4532	T4530	pobj	region,in
R2643	T4533	T4532	amod	embryonic,region
R2644	T4534	T4535	punct	(,5C
R2645	T4535	T4532	parataxis	5C,region
R2646	T4536	T4535	compound	Figure,5C
R2647	T4537	T4535	punct	),5C
R2648	T4538	T4532	cc	and,region
R2649	T4539	T4540	det	the,chorion
R265	T739	T728	relcl	associated,form
R2650	T4540	T4532	conj	chorion,region
R2651	T4541	T4542	punct	(,5D
R2652	T4542	T4540	parataxis	5D,chorion
R2653	T4543	T4542	compound	Figure,5D
R2654	T4544	T4542	punct	),5D
R2655	T4545	T4528	punct	.,was
R2656	T4547	T4548	amod	Detectable,levels
R2657	T4548	T4551	nsubjpass	levels,observed
R2658	T4549	T4547	cc	but,Detectable
R2659	T4550	T4547	conj	lower,Detectable
R266	T740	T739	auxpass	is,associated
R2660	T4552	T4548	prep	of,levels
R2661	T4553	T4552	pobj	expression,of
R2662	T4554	T4551	auxpass	were,observed
R2663	T4555	T4551	prep	in,observed
R2664	T4556	T4557	det	the,cone
R2665	T4557	T4555	pobj	cone,in
R2666	T4558	T4557	amod	ectoplacental,cone
R2667	T4559	T4560	punct	(,5D
R2668	T4560	T4557	parataxis	5D,cone
R2669	T4561	T4560	compound	Figure,5D
R267	T741	T739	advmod	frequently,associated
R2670	T4562	T4560	punct	),5D
R2671	T4563	T4557	cc	and,cone
R2672	T4564	T4565	amod	surrounding,tissue
R2673	T4565	T4557	conj	tissue,cone
R2674	T4566	T4565	amod	decidual,tissue
R2675	T4567	T4551	punct	.,observed
R2676	T4569	T4570	nsubj	We,observed
R2677	T4571	T4570	advmod	also,observed
R2678	T4572	T4573	advmod	very,high
R2679	T4573	T4574	amod	high,levels
R268	T742	T739	prep	with,associated
R2680	T4574	T4570	dobj	levels,observed
R2681	T4575	T4574	prep	of,levels
R2682	T4576	T4577	compound	Atrx,expression
R2683	T4577	T4575	pobj	expression,of
R2684	T4578	T4570	prep	in,observed
R2685	T4579	T4580	nmod	trophoblast,cells
R2686	T4580	T4578	pobj	cells,in
R2687	T4581	T4580	amod	giant,cells
R2688	T4582	T4580	punct	(,cells
R2689	T4583	T4580	appos	TGCs,cells
R269	T743	T744	amod	multiple,abnormalities
R2690	T4584	T4580	punct	),cells
R2691	T4585	T4580	acl	surrounding,cells
R2692	T4586	T4587	det	the,membrane
R2693	T4587	T4585	dobj	membrane,surrounding
R2694	T4588	T4587	poss	Reichert,membrane
R2695	T4589	T4588	case	's,Reichert
R2696	T4590	T4591	punct	(,5E
R2697	T4591	T4570	parataxis	5E,observed
R2698	T4592	T4591	compound	Figure,5E
R2699	T4593	T4591	punct	),5E
R27	T184	T186	compound	helicase,proteins
R270	T744	T742	pobj	abnormalities,with
R2700	T4594	T4570	punct	.,observed
R2701	T4596	T4597	prep	Within,was
R2702	T4598	T4599	det	the,nuclei
R2703	T4599	T4596	pobj	nuclei,Within
R2704	T4600	T4599	amod	large,nuclei
R2705	T4601	T4599	prep	of,nuclei
R2706	T4602	T4603	det	these,TGCs
R2707	T4603	T4601	pobj	TGCs,of
R2708	T4604	T4597	punct	", ",was
R2709	T4605	T4606	det	the,association
R271	T745	T744	amod	congenital,abnormalities
R2710	T4606	T4597	nsubj	association,was
R2711	T4607	T4606	amod	typical,association
R2712	T4608	T4606	amod	nuclear,association
R2713	T4609	T4606	prep	of,association
R2714	T4610	T4609	pobj	Atrx,of
R2715	T4611	T4606	prep	with,association
R2716	T4612	T4611	pobj	blocks,with
R2717	T4613	T4612	prep	of,blocks
R2718	T4614	T4615	amod	pericentromeric,heterochromatin
R2719	T4615	T4613	pobj	heterochromatin,of
R272	T746	T747	punct	[,1
R2720	T4616	T4617	punct	[,15
R2721	T4617	T4606	parataxis	15,association
R2722	T4618	T4617	punct	],15
R2723	T4619	T4620	advmod	clearly,observable
R2724	T4620	T4597	acomp	observable,was
R2725	T4621	T4597	punct	.,was
R2726	T4623	T4624	advmod	Only,staining
R2727	T4624	T4626	nsubjpass	staining,seen
R2728	T4625	T4624	compound	background,staining
R2729	T4627	T4626	auxpass	was,seen
R273	T747	T726	parataxis	1,is
R2730	T4628	T4626	prep	in,seen
R2731	T4629	T4630	det	the,tissues
R2732	T4630	T4628	pobj	tissues,in
R2733	T4631	T4630	amod	corresponding,tissues
R2734	T4632	T4630	amod	Atrxnull,tissues
R2735	T4633	T4630	amod	embryonic,tissues
R2736	T4634	T4635	punct	(,5B
R2737	T4635	T4626	parataxis	5B,seen
R2738	T4636	T4635	compound	Figure,5B
R2739	T4637	T4638	punct	–,5D
R274	T748	T747	punct	],1
R2740	T4638	T4635	prep	5D,5B
R2741	T4639	T4635	punct	),5B
R2742	T4640	T4626	punct	", ",seen
R2743	T4641	T4642	mark	while,was
R2744	T4642	T4626	advcl	was,seen
R2745	T4643	T4642	nsubj	expression,was
R2746	T4644	T4643	prep	in,expression
R2747	T4645	T4646	det	the,tissue
R2748	T4646	T4644	pobj	tissue,in
R2749	T4647	T4646	amod	surrounding,tissue
R275	T749	T726	punct	.,is
R2750	T4648	T4646	amod	decidual,tissue
R2751	T4649	T4646	punct	(,tissue
R2752	T4650	T4646	prep	of,tissue
R2753	T4651	T4652	amod	maternal,origin
R2754	T4652	T4650	pobj	origin,of
R2755	T4653	T4646	punct	),tissue
R2756	T4654	T4642	acomp	normal,was
R2757	T4655	T4642	cc	and,was
R2758	T4656	T4642	conj	served,was
R2759	T4657	T4656	prep	as,served
R276	T751	T752	nsubjpass	It,associated
R2760	T4658	T4659	det	an,control
R2761	T4659	T4657	pobj	control,as
R2762	T4660	T4659	compound	antibody,control
R2763	T4661	T4659	compound	staining,control
R2764	T4662	T4663	punct	(,data
R2765	T4663	T4656	meta	data,served
R2766	T4664	T4663	amod	unpublished,data
R2767	T4665	T4663	punct	),data
R2768	T4666	T4626	punct	.,seen
R2769	T4668	T4669	advmod	Morphologically,reduced
R277	T753	T752	auxpass	is,associated
R2770	T4670	T4669	punct	", ",reduced
R2771	T4671	T4672	nummod	7.5,dpc
R2772	T4672	T4673	nmod	dpc,embryos
R2773	T4673	T4669	nsubjpass	embryos,reduced
R2774	T4674	T4673	amod	Atrxnull,embryos
R2775	T4675	T4669	auxpass	were,reduced
R2776	T4676	T4669	advmod	dramatically,reduced
R2777	T4677	T4669	prep	in,reduced
R2778	T4678	T4677	pobj	size,in
R2779	T4679	T4669	cc	and,reduced
R278	T754	T752	advmod	usually,associated
R2780	T4680	T4669	conj	appeared,reduced
R2781	T4681	T4682	advmod	developmentally,retarded
R2782	T4682	T4680	oprd	retarded,appeared
R2783	T4683	T4680	advcl	relative,appeared
R2784	T4684	T4683	prep	to,relative
R2785	T4685	T4686	npadvmod	stage,matched
R2786	T4686	T4688	amod	matched,embryos
R2787	T4687	T4686	punct	-,matched
R2788	T4688	T4684	pobj	embryos,to
R2789	T4689	T4690	amod	wild,type
R279	T755	T752	prep	with,associated
R2790	T4690	T4688	compound	type,embryos
R2791	T4691	T4690	punct	-,type
R2792	T4692	T4693	punct	(,5A
R2793	T4693	T4680	parataxis	5A,appeared
R2794	T4694	T4693	compound	Figure,5A
R2795	T4695	T4693	cc	and,5A
R2796	T4696	T4693	conj	5B,5A
R2797	T4697	T4693	punct	),5A
R2798	T4698	T4669	punct	.,reduced
R2799	T4700	T4701	advmod	However,appeared
R28	T185	T184	punct	/,helicase
R280	T756	T757	det	a,form
R2800	T4702	T4701	punct	", ",appeared
R2801	T4703	T4701	prep	despite,appeared
R2802	T4704	T4705	poss	their,size
R2803	T4705	T4703	pobj	size,despite
R2804	T4706	T4705	amod	reduced,size
R2805	T4707	T4701	punct	", ",appeared
R2806	T4708	T4709	det	the,morphology
R2807	T4709	T4701	nsubj	morphology,appeared
R2808	T4710	T4709	amod	general,morphology
R2809	T4711	T4709	cc	and,morphology
R281	T757	T755	pobj	form,with
R2810	T4712	T4709	conj	organisation,morphology
R2811	T4713	T4709	prep	of,morphology
R2812	T4714	T4715	amod	embryonic,structures
R2813	T4715	T4713	pobj	structures,of
R2814	T4716	T4709	prep	in,morphology
R2815	T4717	T4718	amod	Atrxnull,conceptuses
R2816	T4718	T4716	pobj	conceptuses,in
R2817	T4719	T4720	advmod	grossly,normal
R2818	T4720	T4701	oprd	normal,appeared
R2819	T4721	T4701	punct	.,appeared
R282	T758	T757	amod	mild,form
R2820	T4723	T4724	det	The,amnion
R2821	T4724	T4725	nsubj	amnion,were
R2822	T4726	T4724	cc	and,amnion
R2823	T4727	T4724	conj	chorion,amnion
R2824	T4728	T4729	advmod	clearly,present
R2825	T4729	T4725	acomp	present,were
R2826	T4730	T4725	cc	and,were
R2827	T4731	T4732	det	the,cavities
R2828	T4732	T4739	nsubj	cavities,were
R2829	T4733	T4732	amod	amniotic,cavities
R283	T759	T757	prep	of,form
R2830	T4734	T4733	punct	", ",amniotic
R2831	T4735	T4733	conj	exocoelomic,amniotic
R2832	T4736	T4735	punct	", ",exocoelomic
R2833	T4737	T4735	cc	and,exocoelomic
R2834	T4738	T4735	conj	ectoplacental,exocoelomic
R2835	T4739	T4725	conj	were,were
R2836	T4740	T4739	acomp	distinguishable,were
R2837	T4741	T4739	punct	", ",were
R2838	T4742	T4743	mark	as,were
R2839	T4743	T4739	advcl	were,were
R284	T760	T761	compound	α,thalassaemia
R2840	T4744	T4745	det	all,layers
R2841	T4745	T4743	nsubj	layers,were
R2842	T4746	T4745	nummod	three,layers
R2843	T4747	T4745	amod	embryonic,layers
R2844	T4748	T4745	compound	germ,layers
R2845	T4749	T4750	punct	(,5A
R2846	T4750	T4739	parataxis	5A,were
R2847	T4751	T4750	compound	Figure,5A
R2848	T4752	T4753	punct	–,5C
R2849	T4753	T4750	prep	5C,5A
R285	T761	T759	pobj	thalassaemia,of
R2850	T4754	T4750	punct	),5A
R2851	T4755	T4739	punct	.,were
R2852	T4757	T4758	prep	At,dissected
R2853	T4759	T4760	nummod	8.5,dpc
R2854	T4760	T4757	pobj	dpc,At
R2855	T4761	T4758	punct	", ",dissected
R2856	T4762	T4758	nsubjpass	embryos,dissected
R2857	T4763	T4758	auxpass	were,dissected
R2858	T4764	T4758	advcl	free,dissected
R2859	T4765	T4764	prep	of,free
R286	T762	T761	punct	-,thalassaemia
R2860	T4766	T4765	pobj	deciduas,of
R2861	T4767	T4758	punct	", ",dissected
R2862	T4768	T4758	cc	and,dissected
R2863	T4769	T4758	conj	observed,dissected
R2864	T4770	T4769	prep	in,observed
R2865	T4771	T4772	amod	whole,mount
R2866	T4772	T4770	pobj	mount,in
R2867	T4773	T4758	punct	.,dissected
R2868	T4775	T4776	amod	Individual,conceptuses
R2869	T4776	T4777	nsubjpass	conceptuses,genotyped
R287	T763	T757	punct	", ",form
R2870	T4778	T4777	auxpass	were,genotyped
R2871	T4779	T4777	prep	by,genotyped
R2872	T4780	T4779	pobj	PCR,by
R2873	T4781	T4777	advcl	using,genotyped
R2874	T4782	T4781	dobj	DNA,using
R2875	T4783	T4782	acl	isolated,DNA
R2876	T4784	T4783	prep	from,isolated
R2877	T4785	T4786	compound	yolk,sac
R2878	T4786	T4784	pobj	sac,from
R2879	T4787	T4788	mark	as,described
R288	T764	T757	acl	caused,form
R2880	T4788	T4777	advcl	described,genotyped
R2881	T4789	T4788	prep	in,described
R2882	T4790	T4791	compound	Protocol,S1
R2883	T4791	T4789	pobj	S1,in
R2884	T4792	T4777	punct	.,genotyped
R2885	T4794	T4795	advcl	Consistent,appeared
R2886	T4796	T4794	prep	with,Consistent
R2887	T4797	T4796	pobj	observations,with
R2888	T4798	T4797	prep	at,observations
R2889	T4799	T4800	nummod	7.5,dpc
R289	T765	T764	agent	by,caused
R2890	T4800	T4798	pobj	dpc,at
R2891	T4801	T4795	punct	", ",appeared
R2892	T4802	T4803	det	the,morphology
R2893	T4803	T4795	nsubj	morphology,appeared
R2894	T4804	T4803	amod	general,morphology
R2895	T4805	T4803	prep	of,morphology
R2896	T4806	T4807	det	the,embryo
R2897	T4807	T4805	pobj	embryo,of
R2898	T4808	T4807	amod	proper,embryo
R2899	T4809	T4807	prep	of,embryo
R29	T186	T182	pobj	proteins,of
R290	T766	T767	amod	reduced,expression
R2900	T4810	T4811	amod	Atrxnull,conceptuses
R2901	T4811	T4809	pobj	conceptuses,of
R2902	T4812	T4795	advmod	also,appeared
R2903	T4813	T4814	advmod	grossly,normal
R2904	T4814	T4795	oprd	normal,appeared
R2905	T4815	T4795	prep	at,appeared
R2906	T4816	T4817	nummod	8.5,dpc
R2907	T4817	T4815	pobj	dpc,at
R2908	T4818	T4795	punct	.,appeared
R2909	T4820	T4821	det	The,fold
R291	T767	T765	pobj	expression,by
R2910	T4821	T4823	nsubj	fold,formed
R2911	T4822	T4821	compound	head,fold
R2912	T4824	T4823	aux	had,formed
R2913	T4825	T4823	advmod	clearly,formed
R2914	T4826	T4823	punct	", ",formed
R2915	T4827	T4823	cc	and,formed
R2916	T4828	T4829	nsubjpass	expression,detected
R2917	T4829	T4823	conj	detected,formed
R2918	T4830	T4828	prep	of,expression
R2919	T4831	T4832	det	the,brachyury
R292	T768	T767	prep	of,expression
R2920	T4832	T4830	pobj	brachyury,of
R2921	T4833	T4832	amod	early,brachyury
R2922	T4834	T4832	compound	mesoderm,brachyury
R2923	T4835	T4832	compound	marker,brachyury
R2924	T4836	T4837	punct	(,T
R2925	T4837	T4832	appos	T,brachyury
R2926	T4838	T4828	punct	),expression
R2927	T4839	T4840	punct	[,16
R2928	T4840	T4828	parataxis	16,expression
R2929	T4841	T4840	punct	],16
R293	T769	T770	advmod	structurally,intact
R2930	T4842	T4829	auxpass	was,detected
R2931	T4843	T4829	prep	in,detected
R2932	T4844	T4845	det	the,streak
R2933	T4845	T4843	pobj	streak,in
R2934	T4846	T4845	amod	primitive,streak
R2935	T4847	T4845	cc	and,streak
R2936	T4848	T4849	amod	emerging,notochord
R2937	T4849	T4845	conj	notochord,streak
R2938	T4850	T4829	prep	by,detected
R2939	T4851	T4852	amod	whole,mount
R294	T770	T771	amod	intact,genes
R2940	T4852	T4854	nmod	mount,hybridisation
R2941	T4853	T4852	punct	-,mount
R2942	T4854	T4850	pobj	hybridisation,by
R2943	T4855	T4856	advmod	in,situ
R2944	T4856	T4854	amod	situ,hybridisation
R2945	T4857	T4854	punct	(,hybridisation
R2946	T4858	T4854	appos	WMISH,hybridisation
R2947	T4859	T4829	punct	),detected
R2948	T4860	T4861	punct	(,5F
R2949	T4861	T4829	parataxis	5F,detected
R295	T771	T768	pobj	genes,of
R2950	T4862	T4861	compound	Figure,5F
R2951	T4863	T4861	punct	),5F
R2952	T4864	T4829	punct	", ",detected
R2953	T4865	T4829	advcl	indicating,detected
R2954	T4866	T4867	mark	that,gastrulated
R2955	T4867	T4865	ccomp	gastrulated,indicating
R2956	T4868	T4869	amod	Atrxnull,embryos
R2957	T4869	T4867	nsubj	embryos,gastrulated
R2958	T4870	T4867	aux	had,gastrulated
R2959	T4871	T4829	punct	.,detected
R296	T772	T773	compound	α,globin
R2960	T4873	T4874	aux	To,investigate
R2961	T4874	T4875	advcl	investigate,analysed
R2962	T4876	T4877	mark	whether,was
R2963	T4877	T4874	ccomp	was,investigate
R2964	T4878	T4879	det	the,size
R2965	T4879	T4877	nsubj	size,was
R2966	T4880	T4879	amod	reduced,size
R2967	T4881	T4879	prep	of,size
R2968	T4882	T4883	det	the,embryos
R2969	T4883	T4881	pobj	embryos,of
R297	T773	T771	compound	globin,genes
R2970	T4884	T4883	amod	Atrxnull,embryos
R2971	T4885	T4877	prep	due,was
R2972	T4886	T4885	pcomp	to,due
R2973	T4887	T4888	det	an,increase
R2974	T4888	T4885	pobj	increase,due
R2975	T4889	T4888	prep	in,increase
R2976	T4890	T4889	pobj	apoptosis,in
R2977	T4891	T4875	punct	", ",analysed
R2978	T4892	T4875	nsubj	we,analysed
R2979	T4893	T4875	dobj	sections,analysed
R298	T774	T773	punct	-,globin
R2980	T4894	T4893	prep	of,sections
R2981	T4895	T4896	npadvmod	paraffin,embedded
R2982	T4896	T4898	amod	embedded,embryos
R2983	T4897	T4896	punct	-,embedded
R2984	T4898	T4894	pobj	embryos,of
R2985	T4899	T4900	nummod	7.5,dpc
R2986	T4900	T4898	compound	dpc,embryos
R2987	T4901	T4875	prep	by,analysed
R2988	T4902	T4903	npadvmod	TdT,mediated
R2989	T4903	T4905	amod	mediated,labeling
R299	T775	T752	punct	", ",associated
R2990	T4904	T4903	punct	-,mediated
R2991	T4905	T4909	nmod	labeling,assay
R2992	T4906	T4905	nmod	dUTP,labeling
R2993	T4907	T4905	nmod	nick,labeling
R2994	T5014	T5013	prep	in,observed
R2995	T5015	T5016	amod	Atrxnull,embryos
R2996	T5016	T5014	pobj	embryos,in
R2997	T5017	T5003	punct	", ",investigated
R2998	T5018	T5019	det	a,defect
R2999	T5019	T5003	nsubjpass	defect,investigated
R3	T156	T155	pobj	Atrx,of
R30	T187	T174	acl	thought,member
R300	T776	T752	cc	and,associated
R3000	T5020	T5019	amod	possible,defect
R3001	T5021	T5019	compound	proliferation,defect
R3002	T5022	T5003	auxpass	was,investigated
R3003	T5023	T5003	advmod	also,investigated
R3004	T5024	T5003	prep	by,investigated
R3005	T5025	T5026	amod	immunohistochemical,staining
R3006	T5026	T5024	pobj	staining,by
R3007	T5027	T5026	prep	of,staining
R3008	T5028	T5029	nummod	7.5,dpc
R3009	T5029	T5030	compound	dpc,embryo
R301	T777	T752	conj	characterised,associated
R3010	T5030	T5031	compound	embryo,sections
R3011	T5031	T5027	pobj	sections,of
R3012	T5032	T5003	prep	for,investigated
R3013	T5033	T5034	det	the,H3
R3014	T5034	T5032	pobj	H3,for
R3015	T5035	T5036	compound	mitosis,marker
R3016	T4908	T4905	nmod	end,labeling
R3017	T5036	T5037	npadvmod	marker,phosphorylated
R3018	T4909	T4901	pobj	assay,by
R3019	T4910	T4905	punct	(,labeling
R302	T778	T777	prep	by,characterised
R3020	T5037	T5034	amod	phosphorylated,H3
R3021	T4911	T4905	appos	TUNEL,labeling
R3022	T4912	T4909	punct	),assay
R3023	T5038	T5039	punct	(,Ser10
R3024	T4913	T4914	punct	(,6A
R3025	T4914	T4875	parataxis	6A,analysed
R3026	T4915	T4914	compound	Figure,6A
R3027	T5039	T5037	parataxis	Ser10,phosphorylated
R3028	T4916	T4914	punct	),6A
R3029	T4917	T4875	punct	.,analysed
R303	T779	T780	det	the,presence
R3030	T4919	T4920	advmod	Very,few
R3031	T4920	T4921	amod	few,cells
R3032	T5040	T5039	punct	),Ser10
R3033	T4921	T4923	nsubjpass	cells,detected
R3034	T4922	T4921	amod	apoptotic,cells
R3035	T5041	T5034	compound	histone,H3
R3036	T4924	T4923	auxpass	were,detected
R3037	T4925	T4923	prep	in,detected
R3038	T5042	T5043	punct	[,13
R3039	T4926	T4927	amod	wild,type
R304	T780	T778	pobj	presence,by
R3040	T4927	T4929	nmod	type,embryos
R3041	T4928	T4927	punct	-,type
R3042	T5043	T5003	parataxis	13,investigated
R3043	T4929	T4925	pobj	embryos,in
R3044	T4930	T4931	nummod	7.5,dpc
R3045	T4931	T4929	compound	dpc,embryos
R3046	T5044	T5043	punct	],13
R3047	T4932	T4923	punct	.,detected
R3048	T5045	T5003	punct	.,investigated
R3049	T4934	T4935	prep	In,was
R305	T781	T780	prep	of,presence
R3050	T4936	T4937	amod	Atrxnull,embryos
R3051	T5047	T5048	advcl	Relative,reduced
R3052	T4937	T4934	pobj	embryos,In
R3053	T4938	T4935	punct	", ",was
R3054	T4939	T4940	det	a,increase
R3055	T5049	T5047	prep	to,Relative
R3056	T4940	T4935	nsubj	increase,was
R3057	T4941	T4940	amod	slight,increase
R3058	T4942	T4940	prep	in,increase
R3059	T4943	T4944	det	the,population
R306	T782	T783	compound	β,globin
R3060	T4944	T4942	pobj	population,in
R3061	T5050	T5051	det	the,index
R3062	T4945	T4944	amod	apoptotic,population
R3063	T4946	T4935	acomp	evident,was
R3064	T4947	T4935	punct	.,was
R3065	T5051	T5049	pobj	index,to
R3066	T4949	T4950	advmod	However,was
R3067	T4951	T4950	punct	", ",was
R3068	T5052	T5053	advmod	very,high
R3069	T4952	T4950	advcl	consistent,was
R307	T783	T785	compound	globin,tetramers
R3070	T4953	T4952	prep	with,consistent
R3071	T4954	T4955	poss	our,observation
R3072	T5053	T5051	amod	high,index
R3073	T4955	T4953	pobj	observation,with
R3074	T4956	T4955	prep	of,observation
R3075	T4957	T4958	det	a,index
R3076	T5054	T5051	amod	mitotic,index
R3077	T4958	T4956	pobj	index,of
R3078	T4959	T4960	advmod	grossly,normal
R3079	T4960	T4958	amod	normal,index
R308	T784	T783	punct	-,globin
R3080	T5055	T5051	acl	observed,index
R3081	T4961	T4958	amod	apoptotic,index
R3082	T4962	T4955	prep	in,observation
R3083	T4963	T4964	amod	Atrxnull,ES
R3084	T4964	T4965	compound	ES,cells
R3085	T4965	T4962	pobj	cells,in
R3086	T5056	T5055	prep	in,observed
R3087	T4966	T4967	punct	(,S2
R3088	T5057	T5058	amod	wild,type
R3089	T4967	T4952	parataxis	S2,consistent
R309	T785	T781	pobj	tetramers,of
R3090	T5058	T5060	compound	type,embryos
R3091	T4968	T4967	compound	Figure,S2
R3092	T4969	T4967	punct	),S2
R3093	T4970	T4950	punct	", ",was
R3094	T5059	T5058	punct	-,type
R3095	T4971	T4972	det	the,response
R3096	T4972	T4950	nsubj	response,was
R3097	T5060	T5056	pobj	embryos,in
R3098	T4973	T4972	amod	apoptotic,response
R3099	T4974	T4972	acl	observed,response
R31	T188	T189	aux	to,regulate
R310	T786	T785	punct	(,tetramers
R3100	T4975	T4974	prep	in,observed
R3101	T5061	T5048	punct	", ",reduced
R3102	T4976	T4977	amod	Atrxnull,embryos
R3103	T4977	T4975	pobj	embryos,in
R3104	T5062	T5063	det	the,proportion
R3105	T4978	T4950	advmod	also,was
R3106	T4979	T4950	neg	not,was
R3107	T4980	T4950	acomp	uniform,was
R3108	T5063	T5048	nsubjpass	proportion,reduced
R3109	T4981	T4950	punct	", ",was
R311	T787	T788	compound	haemoglobin,H
R3110	T4982	T4950	cc	but,was
R3111	T5064	T5063	prep	of,proportion
R3112	T4983	T4984	auxpass	was,restricted
R3113	T4984	T4950	conj	restricted,was
R3114	T4985	T4984	prep	to,restricted
R3115	T5065	T5066	amod	mitotic,cells
R3116	T4986	T4987	det	a,number
R3117	T4987	T4985	pobj	number,to
R3118	T4988	T4987	amod	low,number
R3119	T5066	T5064	pobj	cells,of
R312	T788	T789	compound	H,bodies
R3120	T4989	T4987	prep	of,number
R3121	T4990	T4991	amod	scattered,cells
R3122	T4991	T4989	pobj	cells,of
R3123	T5067	T5066	acl	observed,cells
R3124	T4992	T4993	npadvmod	TUNEL,positive
R3125	T4993	T4991	amod	positive,cells
R3126	T4994	T4993	punct	-,positive
R3127	T5068	T5067	prep	in,observed
R3128	T4995	T4950	punct	.,was
R3129	T5069	T5070	amod	Atrxnull,embryos
R313	T789	T785	appos	bodies,tetramers
R3130	T4997	T4998	mark	Since,is
R3131	T4998	T5003	advcl	is,investigated
R3132	T4999	T5000	det	this,response
R3133	T5070	T5068	pobj	embryos,in
R3134	T5000	T4998	nsubj	response,is
R3135	T5001	T5000	amod	small,response
R3136	T5002	T5000	amod	apoptotic,response
R3137	T5004	T4998	acomp	unlikely,is
R3138	T5005	T5006	aux	to,account
R3139	T5071	T5067	prep	at,observed
R314	T790	T789	compound	inclusion,bodies
R3140	T5006	T5004	xcomp	account,unlikely
R3141	T5007	T5006	advmod	fully,account
R3142	T5008	T5006	prep	for,account
R3143	T5072	T5073	nummod	7.5,dpc
R3144	T5009	T5010	det	the,deficit
R3145	T5010	T5008	pobj	deficit,for
R3146	T5073	T5071	pobj	dpc,at
R3147	T5011	T5010	amod	dramatic,deficit
R3148	T5012	T5010	compound	size,deficit
R3149	T5013	T5010	acl	observed,deficit
R315	T791	T780	punct	),presence
R3150	T5074	T5048	auxpass	was,reduced
R3151	T5075	T5048	advmod	dramatically,reduced
R3152	T5076	T5077	punct	(,6B
R3153	T5077	T5048	parataxis	6B,reduced
R3154	T5078	T5077	compound	Figure,6B
R3155	T5079	T5077	punct	),6B
R3156	T5080	T5048	punct	.,reduced
R3157	T5120	T5119	compound	growth,defect
R3158	T5082	T5083	advcl	Taken,suggest
R3159	T5121	T5117	auxpass	was,observed
R316	T792	T780	prep	in,presence
R3160	T5122	T5117	advmod	also,observed
R3161	T5084	T5082	advmod	together,Taken
R3162	T5124	T5117	prep	in,observed
R3163	T5125	T5126	amod	Atrxnull,cells
R3164	T5126	T5124	pobj	cells,in
R3165	T5085	T5083	punct	", ",suggest
R3166	T5127	T5126	compound	ES,cells
R3167	T5128	T5129	punct	(,3A
R3168	T5129	T5117	parataxis	3A,observed
R3169	T5086	T5087	det	these,results
R317	T793	T794	amod	peripheral,cells
R3170	T5130	T5129	compound	Figure,3A
R3171	T5131	T5129	punct	),3A
R3172	T5087	T5083	nsubj	results,suggest
R3173	T5132	T5123	punct	", ",depleted
R3174	T5133	T5123	prep	in,depleted
R3175	T5134	T5133	pobj	contrast,in
R3176	T5088	T5089	mark	that,reflects
R3177	T5135	T5134	prep	to,contrast
R3178	T5136	T5137	det	the,embryos
R3179	T5089	T5083	ccomp	reflects,suggest
R318	T794	T792	pobj	cells,in
R3180	T5090	T5091	det	the,deficit
R3181	T5137	T5135	pobj	embryos,to
R3182	T5091	T5089	nsubj	deficit,reflects
R3183	T5138	T5137	amod	Atrxnull,embryos
R3184	T5139	T5123	punct	", ",depleted
R3185	T5140	T5141	det	the,index
R3186	T5141	T5123	nsubjpass	index,depleted
R3187	T5092	T5091	compound	size,deficit
R3188	T5142	T5141	amod	mitotic,index
R3189	T5143	T5141	prep	of,index
R319	T795	T794	amod	red,cells
R3190	T5144	T5145	det	the,population
R3191	T5093	T5091	acl	observed,deficit
R3192	T5145	T5143	pobj	population,of
R3193	T5146	T5147	compound	ES,cell
R3194	T5094	T5093	prep	in,observed
R3195	T5147	T5145	compound	cell,population
R3196	T5148	T5141	punct	(,index
R3197	T5095	T5096	amod	Atrxnull,embryos
R3198	T5149	T5150	mark	as,measured
R3199	T5150	T5141	advcl	measured,index
R32	T189	T187	xcomp	regulate,thought
R320	T796	T794	compound	blood,cells
R3200	T5151	T5150	prep	with,measured
R3201	T5096	T5094	pobj	embryos,in
R3202	T5152	T5153	det	the,antibody
R3203	T5153	T5151	pobj	antibody,with
R3204	T5097	T5098	amod	prior,to
R3205	T5154	T5153	amod	same,antibody
R3206	T5155	T5123	punct	),depleted
R3207	T5156	T5123	auxpass	was,depleted
R3208	T5098	T5093	prep	to,observed
R3209	T5157	T5123	neg	not,depleted
R321	T797	T752	punct	.,associated
R3210	T5158	T5159	punct	(,S1B
R3211	T5099	T5098	pobj	lethality,to
R3212	T5159	T5123	parataxis	S1B,depleted
R3213	T5160	T5159	compound	Figure,S1B
R3214	T5161	T5159	punct	),S1B
R3215	T5100	T5089	advmod	largely,reflects
R3216	T5162	T5123	punct	.,depleted
R3217	T5101	T5102	det	a,defect
R3218	T5164	T5165	det	These,observations
R3219	T5165	T5166	nsubj	observations,suggest
R322	T799	T800	compound	Carrier,females
R3220	T5167	T5168	mark	that,is
R3221	T5102	T5089	dobj	defect,reflects
R3222	T5168	T5166	ccomp	is,suggest
R3223	T5169	T5170	det	the,defect
R3224	T5170	T5168	nsubj	defect,is
R3225	T5103	T5102	amod	proliferative,defect
R3226	T5171	T5170	amod	mitotic,defect
R3227	T5172	T5170	acl	observed,defect
R3228	T5104	T5089	punct	", ",reflects
R3229	T5173	T5172	prep	in,observed
R323	T800	T801	nsubj	females,manifest
R3230	T5174	T5173	pobj	embryos,in
R3231	T5175	T5168	acomp	unlikely,is
R3232	T5105	T5089	prep	with,reflects
R3233	T5176	T5177	aux	to,be
R3234	T5177	T5175	xcomp	be,unlikely
R3235	T5106	T5107	det	a,contribution
R3236	T5178	T5179	det	a,effect
R3237	T5179	T5177	attr	effect,be
R3238	T5180	T5179	amod	direct,effect
R3239	T5181	T5179	punct	", ",effect
R324	T802	T801	advmod	occasionally,manifest
R3240	T5182	T5183	npadvmod	cell,autonomous
R3241	T5107	T5105	pobj	contribution,with
R3242	T5183	T5179	amod	autonomous,effect
R3243	T5184	T5183	punct	-,autonomous
R3244	T5185	T5179	prep	of,effect
R3245	T5186	T5187	det	the,absence
R3246	T5108	T5107	amod	minor,contribution
R3247	T5187	T5185	pobj	absence,of
R3248	T5188	T5187	prep	of,absence
R3249	T5109	T5108	cc	but,minor
R325	T803	T804	compound	haemoglobin,H
R3250	T5189	T5188	pobj	Atrx,of
R3251	T5190	T5168	punct	", ",is
R3252	T5110	T5108	conj	indirect,minor
R3253	T5191	T5168	cc	and,is
R3254	T5192	T5168	conj	is,is
R3255	T5193	T5194	advmod	more,likely
R3256	T5111	T5107	prep	from,contribution
R3257	T5194	T5192	acomp	likely,is
R3258	T5112	T5113	amod	increased,apoptosis
R3259	T5195	T5196	aux	to,be
R326	T804	T805	compound	H,inclusions
R3260	T5113	T5111	pobj	apoptosis,from
R3261	T5196	T5194	xcomp	be,likely
R3262	T5197	T5198	det	a,effect
R3263	T5198	T5196	attr	effect,be
R3264	T5114	T5083	punct	.,suggest
R3265	T5199	T5198	amod	secondary,effect
R3266	T5200	T5198	acl	resulting,effect
R3267	T5116	T5117	mark	Although,observed
R3268	T5201	T5200	prep	from,resulting
R3269	T5202	T5203	det	the,failure
R327	T805	T801	dobj	inclusions,manifest
R3270	T5203	T5201	pobj	failure,from
R3271	T5117	T5123	advcl	observed,depleted
R3272	T5204	T5205	aux	to,develop
R3273	T5205	T5203	acl	develop,failure
R3274	T5206	T5207	det	a,trophoblast
R3275	T5118	T5119	det	a,defect
R3276	T5207	T5205	dobj	trophoblast,develop
R3277	T5208	T5207	amod	normal,trophoblast
R3278	T5209	T5210	punct	(,see
R3279	T5119	T5117	nsubjpass	defect,observed
R328	T806	T801	punct	", ",manifest
R3280	T5210	T5194	parataxis	see,likely
R3281	T5211	T5210	advmod	below,see
R3282	T5212	T5210	punct	),see
R3283	T5213	T5166	punct	.,suggest
R3285	T5495	T5496	compound	Trophectoderm,Failure
R3286	T5497	T5496	prep	in,Failure
R3287	T5498	T5499	amod	Atrxnull,Embryos
R3288	T5499	T5497	pobj	Embryos,in
R3289	T5501	T5502	amod	Whole,mount
R329	T807	T801	cc	but,manifest
R3290	T5502	T5504	compound	mount,observation
R3291	T5503	T5502	punct	-,mount
R3292	T5504	T5505	nsubj	observation,revealed
R3293	T5506	T5504	prep	of,observation
R3294	T5507	T5508	nummod	8.5,dpc
R3295	T5508	T5509	compound	dpc,embryos
R3296	T5509	T5506	pobj	embryos,of
R3297	T5510	T5511	mark	that,appeared
R3298	T5511	T5505	ccomp	appeared,revealed
R3299	T5512	T5511	punct	", ",appeared
R33	T190	T191	compound	gene,expression
R330	T808	T801	conj	are,manifest
R3300	T5513	T5511	prep	in,appeared
R3301	T5514	T5513	pobj	contrast,in
R3302	T5515	T5514	prep	to,contrast
R3303	T5516	T5517	det	the,morphology
R3304	T5517	T5515	pobj	morphology,to
R3305	T5518	T5519	advmod	basically,normal
R3306	T5519	T5517	amod	normal,morphology
R3307	T5520	T5521	mark	although,delayed
R3308	T5521	T5519	parataxis	delayed,normal
R3309	T5522	T5517	prep	of,morphology
R331	T809	T808	advmod	otherwise,are
R3310	T5523	T5524	det	the,embryo
R3311	T5524	T5522	pobj	embryo,of
R3312	T5525	T5524	appos	itself,embryo
R3313	T5526	T5511	punct	", ",appeared
R3314	T5527	T5528	det	the,tissues
R3315	T5528	T5511	nsubj	tissues,appeared
R3316	T5529	T5528	amod	extraembryonic,tissues
R3317	T5530	T5528	prep	of,tissues
R3318	T5531	T5532	amod	Atrxnull,conceptuses
R3319	T5532	T5530	pobj	conceptuses,of
R332	T810	T811	advmod	intellectually,normal
R3320	T5533	T5534	advmod	highly,disorganised
R3321	T5534	T5511	oprd	disorganised,appeared
R3322	T5535	T5505	punct	.,revealed
R3323	T5537	T5538	advmod	When,removed
R3324	T5538	T5541	advcl	removed,appeared
R3325	T5539	T5538	nsubjpass	embryos,removed
R3326	T5540	T5538	auxpass	were,removed
R3327	T5542	T5538	prep	from,removed
R3328	T5543	T5542	pobj	deciduas,from
R3329	T5544	T5541	punct	", ",appeared
R333	T811	T808	acomp	normal,are
R3330	T5545	T5546	det	the,layer
R3331	T5546	T5541	nsubj	layer,appeared
R3332	T5547	T5546	amod	surrounding,layer
R3333	T5548	T5546	compound	trophectoderm,layer
R3334	T5549	T5550	advmod	dramatically,reduced
R3335	T5550	T5541	oprd	reduced,appeared
R3336	T5551	T5541	prep	in,appeared
R3337	T5552	T5553	amod	Atrxnull,embryos
R3338	T5553	T5551	pobj	embryos,in
R3339	T5554	T5541	advcl	relative,appeared
R334	T812	T810	cc	and,intellectually
R3340	T5555	T5554	prep	to,relative
R3341	T5556	T5557	amod	wild,type
R3342	T5557	T5559	compound	type,littermates
R3343	T5558	T5557	punct	-,type
R3344	T5559	T5555	pobj	littermates,to
R3345	T5560	T5541	punct	", ",appeared
R3346	T5561	T5541	cc	and,appeared
R3347	T5562	T5563	det	the,cone
R3348	T5563	T5566	nsubj	cone,appeared
R3349	T5564	T5563	amod	underlying,cone
R335	T813	T810	conj	physically,intellectually
R3350	T5565	T5563	amod	ectoplacental,cone
R3351	T5566	T5541	conj	appeared,appeared
R3352	T5567	T5566	oprd	reduced,appeared
R3353	T5568	T5567	cc	and,reduced
R3354	T5569	T5570	advmod	abnormally,shaped
R3355	T5570	T5567	conj	shaped,reduced
R3356	T5571	T5572	punct	(,7A
R3357	T5572	T5566	parataxis	7A,appeared
R3358	T5573	T5572	compound	Figure,7A
R3359	T5574	T5572	punct	),7A
R336	T814	T801	punct	.,manifest
R3360	T5575	T5566	punct	.,appeared
R3361	T5577	T5578	amod	Vacated,deciduas
R3362	T5578	T5579	nsubjpass	deciduas,bisected
R3363	T5580	T5578	acl	surrounding,deciduas
R3364	T5581	T5582	nummod	8.5,dpc
R3365	T5582	T5583	nmod	dpc,embryos
R3366	T5583	T5580	dobj	embryos,surrounding
R3367	T5584	T5585	amod	wild,type
R3368	T5585	T5583	nmod	type,embryos
R3369	T5586	T5585	punct	-,type
R337	T816	T817	nsubj	Studies,demonstrated
R3370	T5587	T5585	cc	and,type
R3371	T5588	T5585	conj	Atrxnull,type
R3372	T5589	T5579	auxpass	were,bisected
R3373	T5590	T5579	cc	and,bisected
R3374	T5591	T5579	conj	analysed,bisected
R3375	T5592	T5591	prep	by,analysed
R3376	T5593	T5592	pobj	WMISH,by
R3377	T5594	T5591	prep	for,analysed
R3378	T5595	T5594	pobj	expression,for
R3379	T5596	T5595	prep	of,expression
R338	T818	T816	prep	of,Studies
R3380	T5597	T5598	amod	placental,lactogen
R3381	T5598	T5596	pobj	lactogen,of
R3382	T5599	T5598	punct	-,lactogen
R3383	T5600	T5598	nummod	1,lactogen
R3384	T5601	T5598	punct	(,lactogen
R3385	T5602	T5598	appos	Pl,lactogen
R3386	T5603	T5602	punct	-,Pl
R3387	T5604	T5602	nummod	1,Pl
R3388	T5605	T5598	punct	),lactogen
R3389	T5606	T5598	punct	", ",lactogen
R339	T819	T820	compound	X,chromosome
R3390	T5607	T5608	det	a,marker
R3391	T5608	T5598	appos	marker,lactogen
R3392	T5609	T5608	prep	of,marker
R3393	T5610	T5611	advmod	terminally,differentiated
R3394	T5611	T5612	amod	differentiated,TGCs
R3395	T5612	T5609	pobj	TGCs,of
R3396	T5613	T5579	punct	.,bisected
R3397	T5615	T5616	det	The,number
R3398	T5616	T5617	nsubj	number,is
R3399	T5618	T5616	prep	of,number
R34	T191	T189	dobj	expression,regulate
R340	T820	T822	compound	chromosome,inactivation
R3400	T5619	T5620	npadvmod	Pl,expressing
R3401	T5620	T5624	amod	expressing,cells
R3402	T5621	T5619	punct	-,Pl
R3403	T5622	T5619	nummod	1,Pl
R3404	T5623	T5620	punct	-,expressing
R3405	T5624	T5618	pobj	cells,of
R3406	T5625	T5624	acl	attached,cells
R3407	T5626	T5625	prep	to,attached
R3408	T5627	T5628	det	the,wall
R3409	T5628	T5626	pobj	wall,to
R341	T821	T820	punct	-,chromosome
R3410	T5629	T5628	amod	decidual,wall
R3411	T5630	T5625	prep	after,attached
R3412	T5631	T5630	pobj	removal,after
R3413	T5632	T5631	prep	of,removal
R3414	T5633	T5634	det	the,embryo
R3415	T5634	T5632	pobj	embryo,of
R3416	T5635	T5636	det	an,indication
R3417	T5636	T5617	attr	indication,is
R3418	T5637	T5636	prep	of,indication
R3419	T5638	T5639	det	the,density
R342	T822	T818	pobj	inactivation,of
R3420	T5639	T5637	pobj	density,of
R3421	T5640	T5639	prep	of,density
R3422	T5641	T5642	compound	trophoblast,cells
R3423	T5642	T5640	pobj	cells,of
R3424	T5643	T5642	acl	surrounding,cells
R3425	T5644	T5645	det	each,site
R3426	T5645	T5643	dobj	site,surrounding
R3427	T5646	T5645	compound	implantation,site
R3428	T5647	T5648	punct	[,17
R3429	T5648	T5617	parataxis	17,is
R343	T823	T822	prep	in,inactivation
R3430	T5649	T5648	punct	],17
R3431	T5650	T5617	punct	.,is
R3432	T5652	T5653	nsubj	We,found
R3433	T5653	T5654	ccomp	found,was
R3434	T5655	T5656	mark	that,depleted
R3435	T5656	T5653	ccomp	depleted,found
R3436	T5657	T5658	det	the,population
R3437	T5658	T5656	nsubjpass	population,depleted
R3438	T5659	T5658	prep	of,population
R3439	T5660	T5661	npadvmod	Pl,expressing
R344	T824	T825	compound	carrier,females
R3440	T5661	T5665	amod	expressing,cells
R3441	T5662	T5660	punct	-,Pl
R3442	T5663	T5660	nummod	1,Pl
R3443	T5664	T5661	punct	-,expressing
R3444	T5665	T5659	pobj	cells,of
R3445	T5666	T5656	auxpass	was,depleted
R3446	T5667	T5656	prep	in,depleted
R3447	T5668	T5669	det	the,sites
R3448	T5669	T5667	pobj	sites,in
R3449	T5670	T5669	amod	decidual,sites
R345	T825	T823	pobj	females,in
R3450	T5671	T5669	compound	implantation,sites
R3451	T5672	T5669	acl	containing,sites
R3452	T5673	T5674	amod	Atrxnull,embryos
R3453	T5674	T5672	dobj	embryos,containing
R3454	T5675	T5656	advcl	relative,depleted
R3455	T5676	T5675	prep	to,relative
R3456	T5677	T5676	pobj	those,to
R3457	T5678	T5677	acl	containing,those
R3458	T5679	T5680	amod	wild,type
R3459	T5680	T5682	compound	type,littermates
R346	T826	T817	aux	have,demonstrated
R3460	T5681	T5680	punct	-,type
R3461	T5682	T5678	dobj	littermates,containing
R3462	T5683	T5684	punct	(,7B
R3463	T5684	T5653	parataxis	7B,found
R3464	T5685	T5684	compound	Figure,7B
R3465	T5686	T5684	punct	),7B
R3466	T5687	T5654	punct	;,was
R3467	T5688	T5654	nsubj	this,was
R3468	T5689	T5654	advmod	also,was
R3469	T5690	T5654	acomp	apparent,was
R347	T827	T828	amod	preferential,inactivation
R3470	T5691	T5654	prep	at,was
R3471	T5692	T5693	nummod	7.5,dpc
R3472	T5693	T5691	pobj	dpc,at
R3473	T5694	T5654	punct	", ",was
R3474	T5695	T5696	mark	as,determined
R3475	T5696	T5654	advcl	determined,was
R3476	T5697	T5696	prep	by,determined
R3477	T5698	T5699	amod	immunohistochemical,staining
R3478	T5699	T5697	pobj	staining,by
R3479	T5700	T5699	prep	of,staining
R348	T828	T817	dobj	inactivation,demonstrated
R3480	T5701	T5702	compound	paraffin,sections
R3481	T5702	T5700	pobj	sections,of
R3482	T5703	T5702	prep	of,sections
R3483	T5704	T5703	pobj	embryos,of
R3484	T5705	T5704	prep	in,embryos
R3485	T5706	T5705	pobj	deciduas,in
R3486	T5707	T5699	prep	with,staining
R3487	T5708	T5709	det	an,antibody
R3488	T5709	T5707	pobj	antibody,with
R3489	T5710	T5711	amod	anti-Pl,1
R349	T829	T828	prep	of,inactivation
R3490	T5711	T5709	nummod	1,antibody
R3491	T5712	T5711	punct	-,1
R3492	T5713	T5714	punct	(,7C
R3493	T5714	T5654	parataxis	7C,was
R3494	T5715	T5714	compound	Figure,7C
R3495	T5716	T5714	punct	),7C
R3496	T5717	T5654	punct	.,was
R3497	T5719	T5720	det	A,deficiency
R3498	T5720	T5722	nsubj	deficiency,is
R3499	T5721	T5720	compound	TGC,deficiency
R35	T192	T189	prep	by,regulate
R350	T830	T831	det	the,chromosome
R3500	T5723	T5722	prep	in,is
R3501	T5724	T5725	det	the,absence
R3502	T5725	T5723	pobj	absence,in
R3503	T5726	T5725	prep	of,absence
R3504	T5727	T5726	pobj	Atrx,of
R3505	T5728	T5722	acomp	consistent,is
R3506	T5729	T5728	prep	with,consistent
R3507	T5730	T5731	det	the,observation
R3508	T5731	T5729	pobj	observation,with
R3509	T5732	T5733	mark	that,is
R351	T831	T829	pobj	chromosome,of
R3510	T5733	T5731	acl	is,observation
R3511	T5734	T5733	nsubj	Atrx,is
R3512	T5735	T5736	advmod	highly,expressed
R3513	T5736	T5733	acomp	expressed,is
R3514	T5737	T5733	prep	in,is
R3515	T5738	T5739	amod	giant,cells
R3516	T5739	T5737	pobj	cells,in
R3517	T5740	T5739	acl	surrounding,cells
R3518	T5741	T5742	amod	wild,type
R3519	T5742	T5744	nmod	type,embryos
R352	T832	T831	acl	bearing,chromosome
R3520	T5743	T5742	punct	-,type
R3521	T5744	T5740	dobj	embryos,surrounding
R3522	T5745	T5746	nummod	7.5,dpc
R3523	T5746	T5744	compound	dpc,embryos
R3524	T5747	T5748	punct	(,5E
R3525	T5748	T5722	parataxis	5E,is
R3526	T5749	T5748	compound	Figure,5E
R3527	T5750	T5748	punct	),5E
R3528	T5751	T5722	punct	.,is
R3529	T5753	T5754	aux	To,investigate
R353	T833	T834	det	the,allele
R3530	T5754	T5755	advcl	investigate,cultured
R3531	T5756	T5757	mark	whether,restricted
R3532	T5757	T5754	ccomp	restricted,investigate
R3533	T5758	T5759	det	the,defect
R3534	T5759	T5757	nsubjpass	defect,restricted
R3535	T5760	T5759	compound	trophoblast,defect
R3536	T5761	T5757	auxpass	was,restricted
R3537	T5762	T5757	prep	to,restricted
R3538	T5763	T5764	det	the,production
R3539	T5764	T5762	pobj	production,to
R354	T834	T832	dobj	allele,bearing
R3540	T5765	T5764	prep	of,production
R3541	T5766	T5767	amod	secondary,TGCs
R3542	T5767	T5765	pobj	TGCs,of
R3543	T5768	T5767	punct	(,TGCs
R3544	T5769	T5767	acl	produced,TGCs
R3545	T5770	T5769	agent	by,produced
R3546	T5771	T5772	compound	diploid,precursors
R3547	T5772	T5770	pobj	precursors,by
R3548	T5773	T5769	prep	in,produced
R3549	T5774	T5775	det	the,cone
R355	T835	T834	amod	abnormal,allele
R3550	T5775	T5773	pobj	cone,in
R3551	T5776	T5775	amod	ectoplacental,cone
R3552	T5777	T5769	cc	and,produced
R3553	T5778	T5769	conj	derived,produced
R3554	T5779	T5778	advmod	originally,derived
R3555	T5780	T5778	prep	from,derived
R3556	T5781	T5782	det	the,trophectoderm
R3557	T5782	T5780	pobj	trophectoderm,from
R3558	T5783	T5782	amod	polar,trophectoderm
R3559	T5784	T5782	acl	overlying,trophectoderm
R356	T836	T817	prep	in,demonstrated
R3560	T5785	T5786	det	the,mass
R3561	T5786	T5784	dobj	mass,overlying
R3562	T5787	T5786	amod	inner,mass
R3563	T5788	T5786	compound	cell,mass
R3564	T5789	T5786	prep	of,mass
R3565	T5790	T5791	det	the,blastocyst
R3566	T5791	T5789	pobj	blastocyst,of
R3567	T5792	T5757	punct	),restricted
R3568	T5793	T5757	cc	or,restricted
R3569	T5794	T5795	advmod	also,affected
R357	T837	T838	det	a,variety
R3570	T5795	T5757	conj	affected,restricted
R3571	T5796	T5797	det	the,production
R3572	T5797	T5795	dobj	production,affected
R3573	T5798	T5797	prep	of,production
R3574	T5799	T5800	amod	primary,TGCs
R3575	T5800	T5798	pobj	TGCs,of
R3576	T5801	T5800	punct	(,TGCs
R3577	T5802	T5800	acl	resulting,TGCs
R3578	T5803	T5802	prep	from,resulting
R3579	T5804	T5803	pobj	differentiation,from
R358	T838	T836	pobj	variety,in
R3580	T5805	T5804	prep	of,differentiation
R3581	T5806	T5807	det	the,trophectoderm
R3582	T5807	T5805	pobj	trophectoderm,of
R3583	T5808	T5807	amod	mural,trophectoderm
R3584	T5809	T5807	prep	of,trophectoderm
R3585	T5810	T5811	det	the,blastocyst
R3586	T5811	T5809	pobj	blastocyst,of
R3587	T5812	T5755	punct	),cultured
R3588	T5813	T5755	punct	", ",cultured
R3589	T5814	T5755	nsubjpass	blastocysts,cultured
R359	T839	T838	prep	of,variety
R3590	T5815	T5816	punct	(,dpc
R3591	T5816	T5814	parataxis	dpc,blastocysts
R3592	T5817	T5816	nummod	3.5,dpc
R3593	T5818	T5816	punct	),dpc
R3594	T5819	T5814	prep	from,blastocysts
R3595	T5820	T5819	pobj	crosses,from
R3596	T5821	T5820	prep	between,crosses
R3597	T5822	T5823	compound	Atrx WT,flox
R3598	T5823	T5825	compound	flox,females
R3599	T5824	T5823	punct	/,flox
R36	T193	T192	pcomp	modifying,by
R360	T840	T839	pobj	tissues,of
R3600	T5825	T5821	pobj	females,between
R3601	T5826	T5825	cc	and,females
R3602	T5827	T5828	compound	GATA1,Cre
R3603	T5828	T5830	npadvmod	Cre,homozygous
R3604	T5829	T5828	punct	-,Cre
R3605	T5830	T5831	amod	homozygous,males
R3606	T5831	T5825	conj	males,females
R3607	T5832	T5831	amod	transgenic,males
R3608	T5833	T5825	punct	(,females
R3609	T5834	T5835	compound	Atrx WT,Y
R361	T841	T842	punct	[,2
R3610	T5835	T5825	appos	Y,females
R3611	T5836	T5835	punct	/,Y
R3612	T5837	T5835	punct	;,Y
R3613	T5838	T5839	compound	GATA1,Cre
R3614	T5839	T5835	appos	Cre,Y
R3615	T5840	T5839	punct	-,Cre
R3616	T5841	T5839	punct	+,Cre
R3617	T5842	T5839	punct	/,Cre
R3618	T5843	T5839	punct	+,Cre
R3619	T5844	T5755	punct	),cultured
R362	T842	T817	parataxis	2,demonstrated
R3620	T5845	T5755	auxpass	were,cultured
R3621	T5846	T5847	advmod	in,vitro
R3622	T5847	T5755	advmod	vitro,cultured
R3623	T5848	T5755	prep	for,cultured
R3624	T5849	T5850	nummod	5,d
R3625	T5850	T5848	pobj	d,for
R3626	T5851	T5852	aux	to,monitor
R3627	T5852	T5755	advcl	monitor,cultured
R3628	T5853	T5852	dobj	outgrowth,monitor
R3629	T5854	T5853	prep	of,outgrowth
R363	T843	T842	punct	],2
R3630	T5855	T5856	det	the,trophoblast
R3631	T5856	T5854	pobj	trophoblast,of
R3632	T5857	T5856	amod	primary,trophoblast
R3633	T5858	T5755	punct	.,cultured
R3634	T5860	T5861	prep	After,scored
R3635	T5862	T5863	nummod	5,d
R3636	T5863	T5860	pobj	d,After
R3637	T5864	T5861	punct	", ",scored
R3638	T5865	T5866	amod	individual,cultures
R3639	T5866	T5861	nsubjpass	cultures,scored
R364	T844	T817	punct	", ",demonstrated
R3640	T5867	T5866	compound	blastocyst,cultures
R3641	T5868	T5861	auxpass	were,scored
R3642	T5869	T5861	prep	for,scored
R3643	T5870	T5871	det	the,extent
R3644	T5871	T5869	pobj	extent,for
R3645	T5872	T5871	prep	of,extent
R3646	T5873	T5874	amod	primary,outgrowth
R3647	T5874	T5872	pobj	outgrowth,of
R3648	T5875	T5874	compound	trophoblast,outgrowth
R3649	T5876	T5861	punct	", ",scored
R365	T845	T817	cc	and,demonstrated
R3650	T5877	T5861	cc	and,scored
R3651	T5878	T5879	det	the,genotype
R3652	T5879	T5881	nsubjpass	genotype,determined
R3653	T5880	T5879	compound	Atrx,genotype
R3654	T5881	T5861	conj	determined,scored
R3655	T5882	T5879	cc	and,genotype
R3656	T5883	T5879	conj	sex,genotype
R3657	T5884	T5883	prep	of,sex
R3658	T5885	T5886	det	the,blastocyst
R3659	T5886	T5884	pobj	blastocyst,of
R366	T846	T847	det	this,skewing
R3660	T5887	T5881	auxpass	were,determined
R3661	T5888	T5881	prep	by,determined
R3662	T5889	T5888	pobj	PCR,by
R3663	T5890	T5861	punct	.,scored
R3664	T5892	T5893	amod	Most,blastocysts
R3665	T5893	T5894	nsubj	blastocysts,hatched
R3666	T5895	T5894	prep	from,hatched
R3667	T5896	T5897	det	the,pellucida
R3668	T5897	T5895	pobj	pellucida,from
R3669	T5898	T5897	compound	zona,pellucida
R367	T847	T848	nsubjpass	skewing,thought
R3670	T5899	T5894	prep	within,hatched
R3671	T5900	T5901	nummod	24,h
R3672	T5901	T5899	pobj	h,within
R3673	T5902	T5894	punct	", ",hatched
R3674	T5903	T5894	cc	and,hatched
R3675	T5904	T5905	compound	trophoblast,cells
R3676	T5905	T5906	nsubjpass	cells,detected
R3677	T5906	T5894	conj	detected,hatched
R3678	T5907	T5905	acl	spreading,cells
R3679	T5908	T5907	prt	out,spreading
R368	T848	T817	conj	thought,demonstrated
R3680	T5909	T5907	prep	from,spreading
R3681	T5910	T5911	det	the,mass
R3682	T5911	T5909	pobj	mass,from
R3683	T5912	T5911	amod	inner,mass
R3684	T5913	T5911	compound	cell,mass
R3685	T5914	T5906	aux	could,detected
R3686	T5915	T5906	advmod	usually,detected
R3687	T5916	T5906	auxpass	be,detected
R3688	T5917	T5906	prep	within,detected
R3689	T5918	T5919	nummod	48,h
R369	T849	T847	prep	of,skewing
R3690	T5919	T5917	pobj	h,within
R3691	T5920	T5919	prep	of,h
R3692	T5921	T5920	pobj	culture,of
R3693	T5922	T5906	punct	.,detected
R3694	T5924	T5925	det	No,difference
R3695	T5925	T5926	nsubjpass	difference,observed
R3696	T5927	T5926	auxpass	was,observed
R3697	T5928	T5926	prep	in,observed
R3698	T5929	T5930	det	the,rate
R3699	T5930	T5928	pobj	rate,in
R37	T194	T193	dobj	chromatin,modifying
R370	T850	T851	compound	X,inactivation
R3700	T5931	T5930	cc	or,rate
R3701	T5932	T5930	conj	extent,rate
R3702	T5933	T5930	prep	of,rate
R3703	T5934	T5935	compound	trophoblast,outgrowth
R3704	T5935	T5933	pobj	outgrowth,of
R3705	T5936	T5926	prep	over,observed
R3706	T5937	T5938	nummod	5,d
R3707	T5938	T5936	pobj	d,over
R3708	T5939	T5938	prep	of,d
R3709	T5940	T5939	pobj	culture,of
R371	T851	T849	pobj	inactivation,of
R3710	T5941	T5926	prep	between,observed
R3711	T5942	T5943	amod	Atrxnull,blastocysts
R3712	T5943	T5941	pobj	blastocysts,between
R3713	T5944	T5945	punct	(,Y
R3714	T5945	T5943	parataxis	Y,blastocysts
R3715	T5946	T5945	amod	Atrx null,Y
R3716	T5947	T5945	punct	/,Y
R3717	T5948	T5945	punct	", ",Y
R3718	T5949	T5950	nsubj	n,6
R3719	T5950	T5945	ccomp	6,Y
R372	T852	T851	punct	-,inactivation
R3720	T5951	T5950	punct	=,6
R3721	T5952	T5945	punct	),Y
R3722	T5953	T5943	cc	and,blastocysts
R3723	T5954	T5943	conj	blastocysts,blastocysts
R3724	T5955	T5954	acl	bearing,blastocysts
R3725	T5956	T5957	det	an,allele
R3726	T5957	T5955	dobj	allele,bearing
R3727	T5958	T5957	compound	Atrx WT,allele
R3728	T5959	T5960	punct	(,6
R3729	T5960	T5954	parataxis	6,blastocysts
R373	T853	T848	auxpass	is,thought
R3730	T5961	T5962	compound	Atrx WT,WT
R3731	T5962	T5964	dep	WT,6
R3732	T5963	T5962	punct	/,WT
R3733	T5964	T5960	dep	6,6
R3734	T5965	T5964	punct	", ",6
R3735	T5966	T5964	nsubj	n,6
R3736	T5967	T5964	punct	=,6
R3737	T5968	T5960	punct	;,6
R3738	T5969	T5970	compound	Atrx WT,null
R3739	T5970	T5972	dep	null,6
R374	T854	T855	aux	to,explain
R3740	T5971	T5970	punct	/,null
R3741	T5972	T5960	dep	6,6
R3742	T5973	T5972	punct	", ",6
R3743	T5974	T5972	nsubj	n,6
R3744	T5975	T5972	punct	=,6
R3745	T5976	T5960	punct	;,6
R3746	T5977	T5978	compound	Atrx WT,Y
R3747	T5978	T5960	dep	Y,6
R3748	T5979	T5978	punct	/,Y
R3749	T5980	T5960	punct	", ",6
R375	T855	T848	xcomp	explain,thought
R3750	T5981	T5960	nsubj	n,6
R3751	T5982	T5960	punct	=,6
R3752	T5983	T5960	punct	),6
R3753	T5984	T5985	punct	(,shown
R3754	T5985	T5954	parataxis	shown,blastocysts
R3755	T5986	T5985	nsubj	examples,shown
R3756	T5987	T5985	prep	in,shown
R3757	T5988	T5989	compound	Figure,7D
R3758	T5989	T5987	pobj	7D,in
R3759	T5990	T5985	punct	),shown
R376	T856	T857	det	the,phenotype
R3760	T5991	T5926	punct	", ",observed
R3761	T5992	T5926	advcl	suggesting,observed
R3762	T5993	T5994	mark	that,involves
R3763	T5994	T5992	ccomp	involves,suggesting
R3764	T5995	T5996	det	the,defect
R3765	T5996	T5994	nsubj	defect,involves
R3766	T5997	T5994	advmod	specifically,involves
R3767	T5998	T5999	det	the,compartment
R3768	T5999	T5994	dobj	compartment,involves
R3769	T6000	T5999	amod	secondary,compartment
R377	T857	T855	dobj	phenotype,explain
R3770	T6001	T5999	amod	giant,compartment
R3771	T6002	T5999	compound	cell,compartment
R3772	T6003	T5926	punct	.,observed
R3773	T6005	T6006	nsubj	This,is
R3774	T6007	T6006	acomp	consistent,is
R3775	T6008	T6007	prep	with,consistent
R3776	T6009	T6010	det	the,observation
R3777	T6010	T6008	pobj	observation,with
R3778	T6011	T6012	mark	that,implant
R3779	T6012	T6010	acl	implant,observation
R378	T858	T857	amod	mild,phenotype
R3780	T6013	T6014	amod	Atrxnull,conceptuses
R3781	T6014	T6012	nsubj	conceptuses,implant
R3782	T6015	T6012	advmod	successfully,implant
R3783	T6016	T6012	cc	and,implant
R3784	T6017	T6012	conj	survive,implant
R3785	T6018	T6017	prep	to,survive
R3786	T6019	T6018	pobj	gastrulation,to
R3787	T6020	T6006	punct	.,is
R3788	T6022	T6023	advcl	Taken,suggest
R3789	T6024	T6022	advmod	together,Taken
R379	T859	T857	acl	observed,phenotype
R3790	T6025	T6023	punct	", ",suggest
R3791	T6026	T6027	det	these,data
R3792	T6027	T6023	nsubj	data,suggest
R3793	T6028	T6029	mark	that,results
R3794	T6029	T6023	ccomp	results,suggest
R3795	T6030	T6029	nsubj	loss,results
R3796	T6031	T6030	prep	of,loss
R3797	T6032	T6031	pobj	Atrx,of
R3798	T6033	T6029	prep	in,results
R3799	T6034	T6035	det	a,defect
R38	T195	T193	prep	at,modifying
R380	T860	T859	prep	in,observed
R3800	T6035	T6033	pobj	defect,in
R3801	T6036	T6035	prep	in,defect
R3802	T6037	T6036	pobj	formation,in
R3803	T6038	T6037	prep	of,formation
R3804	T6039	T6040	det	the,trophoblast
R3805	T6040	T6038	pobj	trophoblast,of
R3806	T6041	T6040	amod	secondary,trophoblast
R3807	T6042	T6043	dep	that,is
R3808	T6043	T6035	relcl	is,defect
R3809	T6044	T6043	acomp	apparent,is
R381	T861	T860	pobj	carriers,in
R3810	T6045	T6043	prep	from,is
R3811	T6046	T6047	nummod	7.5,dpc
R3812	T6047	T6045	pobj	dpc,from
R3813	T6048	T6023	punct	.,suggest
R3814	T6050	T6051	prep	Despite,exhibit
R3815	T6052	T6050	pcomp	initiating,Despite
R3816	T6053	T6054	amod	normal,organisation
R3817	T6054	T6052	dobj	organisation,initiating
R3818	T6055	T6052	prep	in,initiating
R3819	T6056	T6057	det	the,embryo
R382	T862	T848	punct	.,thought
R3820	T6057	T6055	pobj	embryo,in
R3821	T6058	T6057	amod	proper,embryo
R3822	T6059	T6051	punct	", ",exhibit
R3823	T6060	T6061	amod	Atrxnull,conceptuses
R3824	T6061	T6051	nsubj	conceptuses,exhibit
R3825	T6062	T6063	det	a,defect
R3826	T6063	T6051	dobj	defect,exhibit
R3827	T6064	T6063	amod	proliferative,defect
R3828	T6065	T6051	prep	by,exhibit
R3829	T6066	T6067	nummod	7.5,dpc
R383	T864	T865	det	The,syndrome
R3830	T6067	T6065	pobj	dpc,by
R3831	T6068	T6051	cc	and,exhibit
R3832	T6069	T6051	conj	die,exhibit
R3833	T6070	T6069	prep	by,die
R3834	T6071	T6072	quantmod	around,9.5
R3835	T6072	T6073	nummod	9.5,dpc
R3836	T6073	T6070	pobj	dpc,by
R3837	T6074	T6051	punct	", ",exhibit
R3838	T6075	T6076	advmod	probably,due
R3839	T6076	T6051	prep	due,exhibit
R384	T865	T869	nsubjpass	syndrome,caused
R3840	T6077	T6076	pcomp	to,due
R3841	T6078	T6079	det	a,deficit
R3842	T6079	T6076	pobj	deficit,due
R3843	T6080	T6079	amod	nutritional,deficit
R3844	T6081	T6079	acl	resulting,deficit
R3845	T6082	T6081	prep	from,resulting
R3846	T6083	T6082	pobj	failure,from
R3847	T6084	T6085	aux	to,develop
R3848	T6085	T6083	acl	develop,failure
R3849	T6086	T6087	det	a,trophoblast
R385	T866	T867	compound	ATR,X
R3850	T6087	T6085	dobj	trophoblast,develop
R3851	T6088	T6087	amod	normal,trophoblast
R3852	T6089	T6051	punct	.,exhibit
R386	T867	T865	compound	X,syndrome
R3860	T6481	T6480	prep	from,Escape
R3861	T6482	T6483	amod	Imprinted,Inactivation
R3862	T6483	T6481	pobj	Inactivation,from
R3863	T6484	T6483	prep	of,Inactivation
R3864	T6485	T6486	det	the,Allele
R3865	T6486	T6484	pobj	Allele,of
R3866	T6487	T6488	advmod	Paternally,Inherited
R3867	T6488	T6486	amod	Inherited,Allele
R3868	T6489	T6486	compound	Atrx WT,Allele
R3869	T6490	T6480	prep	in,Escape
R387	T868	T867	punct	-,X
R3870	T6491	T6492	amod	Extraembryonic,Tissues
R3871	T6492	T6490	pobj	Tissues,in
R3872	T6493	T6492	prep	of,Tissues
R3873	T6494	T6495	nmod	Carrier,Mice
R3874	T6495	T6493	pobj	Mice,of
R3875	T6496	T6495	amod	Female,Mice
R3876	T6498	T6499	amod	Female,mice
R3877	T6499	T6500	nsubjpass	mice,detected
R3878	T6501	T6499	acl	carrying,mice
R3879	T6502	T6503	det	an,allele
R388	T870	T869	auxpass	is,caused
R3880	T6503	T6501	dobj	allele,carrying
R3881	T6504	T6503	amod	Atrx null,allele
R3882	T6505	T6506	punct	(,cre
R3883	T6506	T6499	parataxis	cre,mice
R3884	T6507	T6508	compound	Atrx WT,null
R3885	T6508	T6506	dep	null,cre
R3886	T6509	T6508	punct	/,null
R3887	T6510	T6506	punct	;,cre
R3888	T6511	T6506	compound	GATA1,cre
R3889	T6512	T6506	punct	-,cre
R389	T871	T869	agent	by,caused
R3890	T6513	T6506	punct	+,cre
R3891	T6514	T6506	punct	/,cre
R3892	T6515	T6506	punct	−,cre
R3893	T6516	T6506	punct	),cre
R3894	T6517	T6500	auxpass	were,detected
R3895	T6518	T6500	prep	at,detected
R3896	T6519	T6520	nummod	9.5,dpc
R3897	T6520	T6518	pobj	dpc,at
R3898	T6521	T6522	punct	(,Table
R3899	T6522	T6500	parataxis	Table,detected
R39	T196	T197	compound	target,loci
R390	T872	T871	pobj	mutations,by
R3900	T6523	T6522	nummod	1,Table
R3901	T6524	T6522	punct	),Table
R3902	T6525	T6500	cc	and,detected
R3903	T6526	T6500	conj	recovered,detected
R3904	T6527	T6526	prep	at,recovered
R3905	T6528	T6527	pobj	birth,at
R3906	T6529	T6530	punct	(,data
R3907	T6530	T6526	meta	data,recovered
R3908	T6531	T6530	amod	unpublished,data
R3909	T6532	T6530	punct	),data
R391	T873	T872	prep	in,mutations
R3910	T6533	T6500	punct	", ",detected
R3911	T6534	T6535	mark	although,was
R3912	T6535	T6500	advcl	was,detected
R3913	T6536	T6535	prep	at,was
R3914	T6537	T6538	preconj	both,points
R3915	T6538	T6536	pobj	points,at
R3916	T6539	T6538	compound	time,points
R3917	T6540	T6541	det	the,number
R3918	T6541	T6535	nsubj	number,was
R3919	T6542	T6541	prep	of,number
R392	T874	T875	det	a,gene
R3920	T6543	T6544	compound	carrier,females
R3921	T6544	T6542	pobj	females,of
R3922	T6545	T6535	acomp	lower,was
R3923	T6546	T6545	prep	than,lower
R3924	T6547	T6546	pobj	that,than
R3925	T6548	T6547	prep	of,that
R3926	T6549	T6550	amod	wild,type
R3927	T6550	T6552	nmod	type,females
R3928	T6551	T6550	punct	-,type
R3929	T6552	T6548	pobj	females,of
R393	T875	T873	pobj	gene,in
R3930	T6553	T6554	punct	(,cre
R3931	T6554	T6550	parataxis	cre,type
R3932	T6555	T6556	compound	Atrx WT,WT
R3933	T6556	T6554	dep	WT,cre
R3934	T6557	T6556	punct	/,WT
R3935	T6558	T6554	punct	;,cre
R3936	T6559	T6554	compound	GATA1,cre
R3937	T6560	T6554	punct	-,cre
R3938	T6561	T6554	punct	+,cre
R3939	T6562	T6554	punct	/,cre
R394	T876	T875	punct	(,gene
R3940	T6563	T6554	punct	−,cre
R3941	T6564	T6554	punct	),cre
R3942	T6565	T6535	punct	", ",was
R3943	T6566	T6535	advcl	suggesting,was
R3944	T6567	T6568	mark	that,died
R3945	T6568	T6566	ccomp	died,suggesting
R3946	T6569	T6570	det	a,proportion
R3947	T6570	T6568	nsubj	proportion,died
R3948	T6571	T6570	prep	of,proportion
R3949	T6572	T6573	nmod	carrier,embryos
R395	T877	T875	appos	ATRX,gene
R3950	T6573	T6571	pobj	embryos,of
R3951	T6574	T6573	amod	female,embryos
R3952	T6575	T6576	advmod	in,utero
R3953	T6576	T6568	advmod	utero,died
R3954	T6577	T6500	punct	.,detected
R3955	T6579	T6580	amod	Surviving,mice
R3956	T6580	T6584	nsubj	mice,were
R3957	T6581	T6580	amod	adult,mice
R3958	T6582	T6580	nmod	carrier,mice
R3959	T6583	T6580	amod	female,mice
R396	T878	T875	punct	),gene
R3960	T6585	T6584	neg	not,were
R3961	T6586	T6587	advmod	phenotypically,normal
R3962	T6587	T6584	acomp	normal,were
R3963	T6588	T6584	cc	and,were
R3964	T6589	T6584	conj	exhibited,were
R3965	T6590	T6591	amod	mild,abnormalities
R3966	T6591	T6589	dobj	abnormalities,exhibited
R3967	T6592	T6591	amod	behavioural,abnormalities
R3968	T6593	T6584	punct	", ",were
R3969	T6594	T6595	mark	although,reproduce
R397	T879	T880	dep	that,comprises
R3970	T6595	T6584	advcl	reproduce,were
R3971	T6596	T6595	nsubj	some,reproduce
R3972	T6597	T6595	aux	could,reproduce
R3973	T6598	T6584	punct	.,were
R3974	T6600	T6601	prep	For,derived
R3975	T6602	T6603	det	all,embryos
R3976	T6603	T6600	pobj	embryos,For
R3977	T6604	T6605	npadvmod	Atrx WT,null
R3978	T6605	T6603	amod	null,embryos
R3979	T6606	T6605	punct	/,null
R398	T880	T875	relcl	comprises,gene
R3980	T6607	T6603	nmod	carrier,embryos
R3981	T6608	T6603	amod	female,embryos
R3982	T6609	T6603	acl	presented,embryos
R3983	T6610	T6609	prep	in,presented
R3984	T6611	T6610	pobj	Table,in
R3985	T6612	T6611	nummod	1,Table
R3986	T6613	T6601	punct	", ",derived
R3987	T6614	T6615	det	the,allele
R3988	T6615	T6601	nsubjpass	allele,derived
R3989	T6616	T6615	compound	Atrx WT,allele
R399	T881	T882	nummod	36,exons
R3990	T6617	T6601	auxpass	was,derived
R3991	T6618	T6601	advmod	paternally,derived
R3992	T6619	T6601	punct	", ",derived
R3993	T6620	T6621	mark	while,derived
R3994	T6621	T6601	advcl	derived,derived
R3995	T6622	T6623	det	the,allele
R3996	T6623	T6621	nsubjpass	allele,derived
R3997	T6624	T6623	amod	Atrx null,allele
R3998	T6625	T6621	auxpass	was,derived
R3999	T6626	T6621	advmod	maternally,derived
R4	T157	T158	compound	Trophoblast,Development
R40	T197	T195	pobj	loci,at
R400	T882	T880	dobj	exons,comprises
R4000	T6627	T6601	punct	.,derived
R4001	T6629	T6630	prep	In,is
R4002	T6631	T6632	det	the,mouse
R4003	T6632	T6629	pobj	mouse,In
R4004	T6633	T6630	punct	", ",is
R4005	T6634	T6635	compound	X,chromosome
R4006	T6635	T6636	compound	chromosome,inactivation
R4007	T6636	T6630	nsubj	inactivation,is
R4008	T6637	T6630	acomp	subject,is
R4009	T6638	T6637	prep	to,subject
R401	T883	T882	acl	spanning,exons
R4010	T6639	T6640	amod	parental,imprinting
R4011	T6640	T6638	pobj	imprinting,to
R4012	T6641	T6640	prep	in,imprinting
R4013	T6642	T6643	det	the,trophectoderm
R4014	T6643	T6641	pobj	trophectoderm,in
R4015	T6644	T6643	cc	and,trophectoderm
R4016	T6645	T6646	amod	primitive,lineages
R4017	T6646	T6643	conj	lineages,trophectoderm
R4018	T6647	T6646	compound	endoderm,lineages
R4019	T6648	T6649	dep	that,give
R402	T884	T885	nummod	300,kb
R4020	T6649	T6643	relcl	give,trophectoderm
R4021	T6650	T6649	dobj	rise,give
R4022	T6651	T6649	prep	to,give
R4023	T6652	T6653	det	the,tissues
R4024	T6653	T6651	pobj	tissues,to
R4025	T6654	T6653	amod	extraembryonic,tissues
R4026	T6655	T6630	punct	", ",is
R4027	T6656	T6630	advcl	resulting,is
R4028	T6657	T6656	prep	in,resulting
R4029	T6658	T6657	pobj	inactivation,in
R403	T885	T883	dobj	kb,spanning
R4030	T6659	T6658	prep	of,inactivation
R4031	T6660	T6661	det	the,chromosome
R4032	T6661	T6659	pobj	chromosome,of
R4033	T6662	T6663	amod	paternal,X
R4034	T6663	T6661	compound	X,chromosome
R4035	T6664	T6661	punct	(,chromosome
R4036	T6665	T6661	appos	Xp,chromosome
R4037	T6666	T6661	punct	),chromosome
R4038	T6667	T6668	punct	[,18
R4039	T6668	T6630	parataxis	18,is
R404	T886	T885	prep	of,kb
R4040	T6669	T6668	punct	],18
R4041	T6670	T6630	punct	.,is
R4042	T6672	T6673	prep	In,is
R4043	T6674	T6672	pobj	contrast,In
R4044	T6675	T6673	punct	", ",is
R4045	T6676	T6673	prep	in,is
R4046	T6677	T6676	pobj	tissues,in
R4047	T6678	T6677	prep	of,tissues
R4048	T6679	T6680	det	the,embryo
R4049	T6680	T6678	pobj	embryo,of
R405	T887	T888	amod	genomic,DNA
R4050	T6681	T6680	amod	proper,embryo
R4051	T6682	T6677	punct	(,tissues
R4052	T6683	T6677	acl	derived,tissues
R4053	T6684	T6683	prep	from,derived
R4054	T6685	T6686	det	the,mass
R4055	T6686	T6684	pobj	mass,from
R4056	T6687	T6686	amod	inner,mass
R4057	T6688	T6686	compound	cell,mass
R4058	T6689	T6686	prep	of,mass
R4059	T6690	T6691	det	the,blastocyst
R406	T888	T886	pobj	DNA,of
R4060	T6691	T6689	pobj	blastocyst,of
R4061	T6692	T6673	punct	),is
R4062	T6693	T6694	compound	X,inactivation
R4063	T6694	T6673	nsubj	inactivation,is
R4064	T6695	T6694	punct	-,inactivation
R4065	T6696	T6673	acomp	random,is
R4066	T6697	T6698	punct	[,19
R4067	T6698	T6673	parataxis	19,is
R4068	T6699	T6698	punct	],19
R4069	T6700	T6673	punct	.,is
R407	T889	T883	prep	at,spanning
R4070	T6702	T6703	advmod	Thus,expected
R4071	T6704	T6703	punct	", ",expected
R4072	T6705	T6703	prep	in,expected
R4073	T6706	T6707	det	the,compartment
R4074	T6707	T6705	pobj	compartment,in
R4075	T6708	T6707	amod	extraembryonic,compartment
R4076	T6709	T6707	prep	of,compartment
R4077	T6710	T6711	npadvmod	Atrx WT,null
R4078	T6711	T6713	amod	null,females
R4079	T6712	T6711	punct	/,null
R408	T890	T891	compound	Chromosome,Xq13.3
R4080	T6713	T6709	pobj	females,of
R4081	T6714	T6713	compound	carrier,females
R4082	T6715	T6703	punct	", ",expected
R4083	T6716	T6717	amod	normal,inactivation
R4084	T6717	T6703	nsubjpass	inactivation,expected
R4085	T6718	T6717	amod	imprinted,inactivation
R4086	T6719	T6717	compound	X,inactivation
R4087	T6720	T6717	punct	-,inactivation
R4088	T6721	T6703	aux	would,expected
R4089	T6722	T6703	auxpass	be,expected
R409	T891	T889	pobj	Xq13.3,at
R4090	T6723	T6724	aux	to,result
R4091	T6724	T6703	xcomp	result,expected
R4092	T6725	T6724	prep	in,result
R4093	T6726	T6725	pobj	silencing,in
R4094	T6727	T6726	prep	of,silencing
R4095	T6728	T6729	det	the,allele
R4096	T6729	T6727	pobj	allele,of
R4097	T6730	T6731	advmod	paternally,derived
R4098	T6731	T6729	amod	derived,allele
R4099	T6732	T6729	compound	Atrx WT,allele
R41	T198	T172	punct	.,is
R410	T892	T893	punct	[,3
R4100	T6733	T6703	punct	", ",expected
R4101	T6734	T6703	advcl	leaving,expected
R4102	T6735	T6736	advmod	only,allele
R4103	T6736	T6734	dobj	allele,leaving
R4104	T6737	T6736	det	the,allele
R4105	T6738	T6736	amod	Atrx null,allele
R4106	T6739	T6734	prep	on,leaving
R4107	T6740	T6741	det	the,X
R4108	T6741	T6739	pobj	X,on
R4109	T6742	T6741	amod	active,X
R411	T893	T869	parataxis	3,caused
R4110	T6743	T6741	amod	maternal,X
R4111	T6744	T6741	punct	(,X
R4112	T6745	T6741	appos	Xm,X
R4113	T6746	T6734	punct	),leaving
R4114	T6747	T6734	cc	and,leaving
R4115	T6748	T6749	advmod	thereby,render
R4116	T6749	T6734	conj	render,leaving
R4117	T6750	T6751	det	the,tissues
R4118	T6751	T6749	dobj	tissues,render
R4119	T6752	T6751	amod	extraembryonic,tissues
R412	T894	T893	punct	],3
R4120	T6753	T6749	oprd	null,render
R4121	T6754	T6753	prep	for,null
R4122	T6755	T6756	amod	full,length
R4123	T6756	T6758	compound	length,protein
R4124	T6757	T6756	punct	-,length
R4125	T6758	T6754	pobj	protein,for
R4126	T6759	T6758	compound	Atrx,protein
R4127	T6760	T6703	punct	.,expected
R4128	T6762	T6763	advmod	However,is
R4129	T6764	T6763	punct	", ",is
R413	T895	T869	punct	.,caused
R4130	T6765	T6766	det	the,absence
R4131	T6766	T6763	nsubj	absence,is
R4132	T6767	T6766	prep	of,absence
R4133	T6768	T6767	pobj	Atrx,of
R4134	T6769	T6766	prep	in,absence
R4135	T6770	T6771	det	the,compartment
R4136	T6771	T6769	pobj	compartment,in
R4137	T6772	T6771	amod	extraembryonic,compartment
R4138	T6773	T6763	acomp	lethal,is
R4139	T6774	T6763	prep	in,is
R414	T897	T898	det	This,gene
R4140	T6775	T6776	amod	Atrx null,Y
R4141	T6776	T6778	compound	Y,males
R4142	T6777	T6776	punct	/,Y
R4143	T6778	T6774	pobj	males,in
R4144	T6779	T6763	punct	.,is
R4145	T6781	T6782	nsubj	This,suggested
R4146	T6783	T6784	det	the,possibility
R4147	T6784	T6782	dobj	possibility,suggested
R4148	T6785	T6784	prep	of,possibility
R4149	T6786	T6787	det	an,escape
R415	T898	T899	nsubj	gene,encodes
R4150	T6787	T6785	pobj	escape,of
R4151	T6788	T6787	prep	from,escape
R4152	T6789	T6790	amod	imprinted,inactivation
R4153	T6790	T6788	pobj	inactivation,from
R4154	T6791	T6790	prep	of,inactivation
R4155	T6792	T6793	det	the,allele
R4156	T6793	T6791	pobj	allele,of
R4157	T6794	T6795	advmod	paternally,derived
R4158	T6795	T6793	amod	derived,allele
R4159	T6796	T6793	compound	Atrx WT,allele
R416	T900	T901	nummod	two,isoforms
R4160	T6797	T6782	prep	in,suggested
R4161	T6798	T6799	det	the,compartment
R4162	T6799	T6797	pobj	compartment,in
R4163	T6800	T6799	amod	extraembryonic,compartment
R4164	T6801	T6799	prep	of,compartment
R4165	T6802	T6803	det	a,proportion
R4166	T6803	T6801	pobj	proportion,of
R4167	T6804	T6803	prep	of,proportion
R4168	T6805	T6806	nmod	carrier,embryos
R4169	T6806	T6804	pobj	embryos,of
R417	T901	T899	dobj	isoforms,encodes
R4170	T6807	T6806	amod	female,embryos
R4171	T6808	T6782	punct	.,suggested
R4172	T6810	T6811	aux	To,investigate
R4173	T6811	T6812	advcl	investigate,crossed
R4174	T6813	T6811	advmod	further,investigate
R4175	T6814	T6812	punct	", ",crossed
R4176	T6815	T6812	nsubj	we,crossed
R4177	T6816	T6817	amod	homozygous,females
R4178	T6817	T6812	dobj	females,crossed
R4179	T6818	T6819	compound	Atrx flox,flox
R418	T902	T901	amod	dominant,isoforms
R4180	T6819	T6817	compound	flox,females
R4181	T6820	T6819	punct	/,flox
R4182	T6821	T6817	cc	and,females
R4183	T6822	T6823	amod	homozygous,males
R4184	T6823	T6817	conj	males,females
R4185	T6824	T6825	nmod	GATA1,cre
R4186	T6825	T6823	nmod	cre,males
R4187	T6826	T6825	punct	-,cre
R4188	T6827	T6823	amod	transgenic,males
R4189	T6828	T6829	punct	(,cre
R419	T903	T901	compound	protein,isoforms
R4190	T6829	T6823	parataxis	cre,males
R4191	T6830	T6831	compound	Atrx WT,Y
R4192	T6831	T6829	dep	Y,cre
R4193	T6832	T6831	punct	/,Y
R4194	T6833	T6829	punct	;,cre
R4195	T6834	T6829	compound	GATA1,cre
R4196	T6835	T6829	punct	-,cre
R4197	T6836	T6829	punct	+,cre
R4198	T6837	T6829	punct	/,cre
R4199	T6838	T6829	punct	+,cre
R42	T200	T201	nsubj	Mutations,provided
R420	T904	T905	punct	(,Figure
R4200	T6839	T6829	punct	),cre
R4201	T6840	T6812	punct	", ",crossed
R4202	T6841	T6842	dep	which,expected
R4203	T6842	T6812	advcl	expected,crossed
R4204	T6843	T6842	aux	would,expected
R4205	T6844	T6842	auxpass	be,expected
R4206	T6845	T6846	aux	to,yield
R4207	T6846	T6842	xcomp	yield,expected
R4208	T6847	T6848	advmod	only,males
R4209	T6848	T6846	dobj	males,yield
R421	T905	T899	parataxis	Figure,encodes
R4210	T6849	T6848	amod	Atrxnull,males
R4211	T6850	T6851	punct	(,cre
R4212	T6851	T6848	parataxis	cre,males
R4213	T6852	T6853	amod	Atrxnull,Y
R4214	T6853	T6851	dep	Y,cre
R4215	T6854	T6853	punct	/,Y
R4216	T6855	T6851	punct	;,cre
R4217	T6856	T6851	compound	GATA1,cre
R4218	T6857	T6851	punct	-,cre
R4219	T6858	T6851	punct	+,cre
R422	T906	T905	nummod	1,Figure
R4220	T6859	T6851	punct	/,cre
R4221	T6860	T6851	punct	−,cre
R4222	T6861	T6851	punct	),cre
R4223	T6862	T6848	cc	and,males
R4224	T6863	T6864	compound	carrier,females
R4225	T6864	T6848	conj	females,males
R4226	T6865	T6866	punct	(,cre
R4227	T6866	T6864	parataxis	cre,females
R4228	T6867	T6868	compound	Atrx WT,null
R4229	T6868	T6866	dep	null,cre
R423	T907	T905	punct	),Figure
R4230	T6869	T6868	punct	/,null
R4231	T6870	T6866	punct	;,cre
R4232	T6871	T6866	compound	GATA1,cre
R4233	T6872	T6866	punct	-,cre
R4234	T6873	T6866	punct	+,cre
R4235	T6874	T6866	punct	/,cre
R4236	T6875	T6866	punct	−,cre
R4237	T6876	T6866	punct	),cre
R4238	T6877	T6812	punct	.,crossed
R4239	T6879	T6880	prep	In,inherited
R424	T908	T899	punct	.,encodes
R4240	T6881	T6882	det	these,females
R4241	T6882	T6879	pobj	females,In
R4242	T6883	T6882	compound	carrier,females
R4243	T6884	T6880	punct	", ",inherited
R4244	T6885	T6886	det	the,allele
R4245	T6886	T6880	nsubjpass	allele,inherited
R4246	T6887	T6886	compound	Atrx WT,allele
R4247	T6888	T6880	auxpass	is,inherited
R4248	T6889	T6880	advmod	paternally,inherited
R4249	T6890	T6880	punct	.,inherited
R425	T910	T911	advmod	As,as
R4250	T6892	T6893	nsubjpass	Embryos,dissected
R4251	T6894	T6893	auxpass	were,dissected
R4252	T6895	T6893	prep	in,dissected
R4253	T6896	T6897	poss	their,deciduas
R4254	T6897	T6895	pobj	deciduas,in
R4255	T6898	T6893	prep	at,dissected
R4256	T6899	T6900	nummod	7.5,dpc
R4257	T6900	T6898	pobj	dpc,at
R4258	T6901	T6893	punct	", ",dissected
R4259	T6902	T6893	cc	and,dissected
R426	T911	T913	prep	as,demonstrated
R4260	T6903	T6904	compound	paraffin,sections
R4261	T6904	T6905	nsubjpass	sections,stained
R4262	T6905	T6893	conj	stained,dissected
R4263	T6906	T6905	auxpass	were,stained
R4264	T6907	T6905	prep	with,stained
R4265	T6908	T6909	amod	anti-ATRX,antibody
R4266	T6909	T6907	pobj	antibody,with
R4267	T6910	T6905	punct	", ",stained
R4268	T6911	T6912	cc	along,sections
R4269	T6912	T6905	dep	sections,stained
R427	T912	T911	advmod	well,as
R4270	T6913	T6911	dep	with,along
R4271	T6914	T6912	prep	from,sections
R4272	T6915	T6916	det	a,embryo
R4273	T6916	T6914	pobj	embryo,from
R4274	T6917	T6918	amod	wild,type
R4275	T6918	T6916	nmod	type,embryo
R4276	T6919	T6918	punct	-,type
R4277	T6920	T6921	nummod	7.5,dpc
R4278	T6921	T6916	compound	dpc,embryo
R4279	T6922	T6912	prep	for,sections
R428	T914	T915	det	the,protein
R4280	T6923	T6922	pobj	comparison,for
R4281	T6924	T6925	punct	(,Figure
R4282	T6925	T6905	parataxis	Figure,stained
R4283	T6926	T6925	nummod	8,Figure
R4284	T6927	T6925	punct	),Figure
R4285	T6928	T6905	punct	.,stained
R4286	T6930	T6931	mark	As,described
R4287	T6931	T6932	advcl	described,detected
R4288	T6933	T6931	advmod	above,described
R4289	T6934	T6932	punct	", ",detected
R429	T915	T911	pobj	protein,as
R4290	T6935	T6936	compound	Atrx,expression
R4291	T6936	T6932	nsubjpass	expression,detected
R4292	T6937	T6932	auxpass	was,detected
R4293	T6938	T6932	prep	in,detected
R4294	T6939	T6940	det	every,cell
R4295	T6940	T6938	pobj	cell,in
R4296	T6941	T6940	prep	in,cell
R4297	T6942	T6943	det	the,region
R4298	T6943	T6941	pobj	region,in
R4299	T6944	T6943	nmod	epiblast,region
R43	T202	T200	prep	in,Mutations
R430	T916	T917	amod	full,length
R4300	T6945	T6944	punct	(,epiblast
R4301	T6946	T6944	appos	embryo,epiblast
R4302	T6947	T6946	amod	proper,embryo
R4303	T6948	T6943	punct	),region
R4304	T6949	T6943	prep	of,region
R4305	T6950	T6951	amod	wild,type
R4306	T6951	T6953	nmod	type,embryos
R4307	T6952	T6951	punct	-,type
R4308	T6953	T6949	pobj	embryos,of
R4309	T6954	T6955	nummod	7.5,dpc
R431	T917	T915	compound	length,protein
R4310	T6955	T6953	compound	dpc,embryos
R4311	T6956	T6957	punct	(,8B
R4312	T6957	T6932	parataxis	8B,detected
R4313	T6958	T6957	compound	Figure,8B
R4314	T6959	T6957	punct	),8B
R4315	T6960	T6932	punct	.,detected
R4316	T6962	T6963	prep	In,composed
R4317	T6964	T6962	pobj	contrast,In
R4318	T6965	T6963	punct	", ",composed
R4319	T6966	T6967	det	the,region
R432	T918	T917	punct	-,length
R4320	T6967	T6963	nsubjpass	region,composed
R4321	T6968	T6967	compound	epiblast,region
R4322	T6969	T6967	prep	of,region
R4323	T6970	T6971	nmod	carrier,embryos
R4324	T6971	T6969	pobj	embryos,of
R4325	T6972	T6971	amod	female,embryos
R4326	T6973	T6963	auxpass	was,composed
R4327	T6974	T6963	prep	of,composed
R4328	T6975	T6976	det	a,mosaic
R4329	T6976	T6974	pobj	mosaic,of
R433	T919	T915	compound	ATRX,protein
R4330	T6977	T6976	prep	of,mosaic
R4331	T6978	T6979	amod	small,clusters
R4332	T6979	T6977	pobj	clusters,of
R4333	T6980	T6979	prep	of,clusters
R4334	T6981	T6982	npadvmod	Atrx,positive
R4335	T6982	T6984	amod	positive,cells
R4336	T6983	T6982	punct	-,positive
R4337	T6984	T6980	pobj	cells,of
R4338	T6985	T6984	punct	(,cells
R4339	T6986	T6987	prep	in,inactivated
R434	T920	T915	prep	of,protein
R4340	T6987	T6984	relcl	inactivated,cells
R4341	T6988	T6986	pobj	which,in
R4342	T6989	T6990	det	the,allele
R4343	T6990	T6987	nsubjpass	allele,inactivated
R4344	T6991	T6990	amod	Atrx null,allele
R4345	T6992	T6990	prep	on,allele
R4346	T6993	T6992	pobj	Xm,on
R4347	T6994	T6987	aux	had,inactivated
R4348	T6995	T6987	auxpass	been,inactivated
R4349	T6996	T6984	punct	),cells
R435	T921	T922	punct	~,280
R4350	T6997	T6984	cc	and,cells
R4351	T6998	T6999	npadvmod	Atrx,negative
R4352	T6999	T7001	amod	negative,cells
R4353	T7000	T6999	punct	-,negative
R4354	T7001	T6984	conj	cells,cells
R4355	T7002	T7001	punct	(,cells
R4356	T7003	T7004	prep	in,inactivated
R4357	T7004	T7001	relcl	inactivated,cells
R4358	T7005	T7003	pobj	which,in
R4359	T7006	T7007	det	the,allele
R436	T922	T923	nummod	280,kDa
R4360	T7007	T7004	nsubjpass	allele,inactivated
R4361	T7008	T7007	compound	Atrx WT,allele
R4362	T7009	T7007	prep	on,allele
R4363	T7010	T7009	pobj	Xp,on
R4364	T7011	T7004	aux	had,inactivated
R4365	T7012	T7004	auxpass	been,inactivated
R4366	T7013	T6963	punct	),composed
R4367	T7014	T6963	punct	", ",composed
R4368	T7015	T6963	advcl	indicating,composed
R4369	T7016	T7017	mark	that,was
R437	T923	T920	pobj	kDa,of
R4370	T7017	T7015	ccomp	was,indicating
R4371	T7018	T7019	det	the,gene
R4372	T7019	T7017	nsubj	gene,was
R4373	T7020	T7019	compound	Atrx,gene
R4374	T7021	T7017	acomp	subject,was
R4375	T7022	T7021	prep	to,subject
R4376	T7023	T7024	amod	normal,inactivation
R4377	T7024	T7022	pobj	inactivation,to
R4378	T7025	T7024	amod	random,inactivation
R4379	T7026	T7024	compound	X,inactivation
R438	T924	T915	punct	", ",protein
R4380	T7027	T7024	punct	-,inactivation
R4381	T7028	T7017	prep	in,was
R4382	T7029	T7030	det	the,epiblast
R4383	T7030	T7028	pobj	epiblast,in
R4384	T7031	T6963	punct	.,composed
R4385	T7033	T7034	advmod	Remarkably,detected
R4386	T7035	T7034	punct	", ",detected
R4387	T7036	T7037	amod	clear,expression
R4388	T7037	T7034	nsubjpass	expression,detected
R4389	T7038	T7037	compound	Atrx,expression
R439	T925	T926	dep	which,encoded
R4390	T7039	T7034	aux	could,detected
R4391	T7040	T7034	advmod	also,detected
R4392	T7041	T7034	auxpass	be,detected
R4393	T7042	T7034	prep	in,detected
R4394	T7043	T7044	det	the,tissues
R4395	T7044	T7042	pobj	tissues,in
R4396	T7045	T7044	amod	extraembryonic,tissues
R4397	T7046	T7044	prep	of,tissues
R4398	T7047	T7048	compound	carrier,females
R4399	T7048	T7046	pobj	females,of
R44	T203	T202	pobj	ATRX,in
R440	T926	T915	relcl	encoded,protein
R4400	T7049	T7034	punct	", ",detected
R4401	T7050	T7051	mark	as,shown
R4402	T7051	T7034	advcl	shown,detected
R4403	T7052	T7051	prep	in,shown
R4404	T7053	T7054	det	the,ectoderm
R4405	T7054	T7052	pobj	ectoderm,in
R4406	T7055	T7056	amod	extraembryonic,derived
R4407	T7056	T7054	amod	derived,ectoderm
R4408	T7057	T7056	punct	-,derived
R4409	T7058	T7054	amod	chorionic,ectoderm
R441	T927	T926	auxpass	is,encoded
R4410	T7059	T7060	punct	(,8C
R4411	T7060	T7034	parataxis	8C,detected
R4412	T7061	T7060	compound	Figure,8C
R4413	T7062	T7060	punct	),8C
R4414	T7063	T7034	punct	.,detected
R4415	T7065	T7066	compound	Atrx,expression
R4416	T7066	T7067	nsubjpass	expression,detected
R4417	T7068	T7067	aux	could,detected
R4418	T7069	T7067	auxpass	be,detected
R4419	T7070	T7067	prep	in,detected
R442	T928	T926	agent	by,encoded
R4420	T7071	T7072	advmod	almost,all
R4421	T7072	T7073	nummod	all,cells
R4422	T7073	T7070	pobj	cells,in
R4423	T7074	T7073	prep	of,cells
R4424	T7075	T7076	det	the,ectoderm
R4425	T7076	T7074	pobj	ectoderm,of
R4426	T7077	T7076	amod	chorionic,ectoderm
R4427	T7078	T7067	punct	.,detected
R4428	T7080	T7081	compound	Atrx,expression
R4429	T7081	T7082	nsubjpass	expression,detected
R443	T929	T930	det	a,transcript
R4430	T7083	T7082	auxpass	was,detected
R4431	T7084	T7082	advmod	also,detected
R4432	T7085	T7082	advmod	clearly,detected
R4433	T7086	T7082	prep	in,detected
R4434	T7087	T7088	amod	other,structures
R4435	T7088	T7086	pobj	structures,in
R4436	T7089	T7088	amod	extraembryonic,structures
R4437	T7090	T7088	punct	", ",structures
R4438	T7091	T7088	prep	including,structures
R4439	T7092	T7091	pobj	TGCs,including
R444	T930	T928	pobj	transcript,by
R4440	T7093	T7094	punct	(,data
R4441	T7094	T7082	meta	data,detected
R4442	T7095	T7094	amod	unpublished,data
R4443	T7096	T7094	punct	),data
R4444	T7097	T7082	punct	.,detected
R4445	T7099	T7100	nsubjpass	Escape,observed
R4446	T7101	T7099	prep	from,Escape
R4447	T7102	T7101	pobj	silencing,from
R4448	T7103	T7102	prep	of,silencing
R4449	T7104	T7105	det	an,allele
R445	T931	T930	prep	of,transcript
R4450	T7105	T7103	pobj	allele,of
R4451	T7106	T7107	npadvmod	Xp,inherited
R4452	T7107	T7105	amod	inherited,allele
R4453	T7108	T7107	punct	-,inherited
R4454	T7109	T7105	compound	Atrx WT,allele
R4455	T7110	T7100	auxpass	was,observed
R4456	T7111	T7100	advmod	also,observed
R4457	T7112	T7100	prep	in,observed
R4458	T7113	T7114	det	the,ectoderm
R4459	T7114	T7112	pobj	ectoderm,in
R446	T932	T933	punct	~,10
R4460	T7115	T7114	amod	chorionic,ectoderm
R4461	T7116	T7114	prep	of,ectoderm
R4462	T7117	T7118	compound	carrier,females
R4463	T7118	T7116	pobj	females,of
R4464	T7119	T7100	prep	at,observed
R4465	T7120	T7121	nummod	8.5,dpc
R4466	T7121	T7119	pobj	dpc,at
R4467	T7122	T7123	punct	(,S4
R4468	T7123	T7100	parataxis	S4,observed
R4469	T7124	T7123	compound	Figure,S4
R447	T933	T934	nummod	10,kb
R4470	T7125	T7123	punct	),S4
R4471	T7126	T7100	punct	.,observed
R4472	T7128	T7129	advmod	Thus,escaped
R4473	T7130	T7129	punct	", ",escaped
R4474	T7131	T7132	mark	although,occurs
R4475	T7132	T7129	advcl	occurs,escaped
R4476	T7133	T7134	amod	random,inactivation
R4477	T7134	T7132	nsubj	inactivation,occurs
R4478	T7135	T7134	compound	X,inactivation
R4479	T7136	T7134	punct	-,inactivation
R448	T934	T931	pobj	kb,of
R4480	T7137	T7132	advmod	normally,occurs
R4481	T7138	T7132	prep	within,occurs
R4482	T7139	T7140	det	the,epiblast
R4483	T7140	T7138	pobj	epiblast,within
R4484	T7141	T7129	punct	", ",escaped
R4485	T7142	T7143	det	the,allele
R4486	T7143	T7129	nsubj	allele,escaped
R4487	T7144	T7143	compound	Atrx WT,allele
R4488	T7145	T7143	punct	(,allele
R4489	T7146	T7143	acl	inherited,allele
R449	T935	T913	punct	", ",demonstrated
R4490	T7147	T7146	prep	on,inherited
R4491	T7148	T7149	det	the,chromosome
R4492	T7149	T7147	pobj	chromosome,on
R4493	T7150	T7149	compound	Xp,chromosome
R4494	T7151	T7129	punct	),escaped
R4495	T7152	T7153	det	the,inactivation
R4496	T7153	T7129	dobj	inactivation,escaped
R4497	T7154	T7153	amod	normal,inactivation
R4498	T7155	T7153	amod	imprinted,inactivation
R4499	T7156	T7153	compound	X,inactivation
R45	T204	T205	det	the,example
R450	T936	T913	nsubj	we,demonstrated
R4500	T7157	T7153	punct	-,inactivation
R4501	T7158	T7153	prep	in,inactivation
R4502	T7159	T7160	det	the,compartment
R4503	T7160	T7158	pobj	compartment,in
R4504	T7161	T7160	amod	extraembryonic,compartment
R4505	T7162	T7160	prep	of,compartment
R4506	T7163	T7164	det	some,females
R4507	T7164	T7162	pobj	females,of
R4508	T7165	T7164	compound	carrier,females
R4509	T7166	T7129	punct	.,escaped
R451	T937	T913	advmod	recently,demonstrated
R4510	T7194	T7195	nsubj	We,investigated
R4511	T7196	T7197	det	the,role
R4512	T7197	T7195	dobj	role,investigated
R4513	T7198	T7197	prep	of,role
R4514	T7199	T7200	det	the,protein
R4515	T7200	T7198	pobj	protein,of
R4516	T7201	T7200	compound	Atrx,protein
R4517	T7202	T7197	prep	in,role
R4518	T7203	T7204	compound	mouse,development
R4519	T7204	T7202	pobj	development,in
R452	T938	T939	mark	that,produced
R4520	T7205	T7195	punct	.,investigated
R4521	T7207	T7208	prep	By,ablated
R4522	T7209	T7207	pcomp	using,By
R4523	T7210	T7211	det	a,approach
R4524	T7211	T7209	dobj	approach,using
R4525	T7212	T7211	amod	conditional,approach
R4526	T7213	T7211	compound	knockout,approach
R4527	T7214	T7208	punct	", ",ablated
R4528	T7215	T7208	nsubj	we,ablated
R4529	T7216	T7217	det	the,protein
R453	T939	T913	ccomp	produced,demonstrated
R4530	T7217	T7208	dobj	protein,ablated
R4531	T7218	T7219	amod	full,length
R4532	T7219	T7217	compound	length,protein
R4533	T7220	T7219	punct	-,length
R4534	T7221	T7217	compound	Atrx,protein
R4535	T7222	T7223	advmod	first,in
R4536	T7223	T7208	prep	in,ablated
R4537	T7224	T7225	compound	ES,cells
R4538	T7225	T7223	pobj	cells,in
R4539	T7226	T7225	cc	and,cells
R454	T940	T941	det	a,isoform
R4540	T7227	T7228	amod	embryoid,bodies
R4541	T7228	T7225	conj	bodies,cells
R4542	T7229	T7223	punct	", ",in
R4543	T7230	T7223	cc	and,in
R4544	T7231	T7232	advmod	then,in
R4545	T7232	T7223	conj	in,in
R4546	T7233	T7234	amod	developing,embryos
R4547	T7234	T7232	pobj	embryos,in
R4548	T7235	T7234	compound	mouse,embryos
R4549	T7236	T7208	punct	.,ablated
R455	T941	T939	nsubjpass	isoform,produced
R4550	T7416	T7415	prep	in,Atrx
R4551	T7417	T7418	compound	ES,Cells
R4552	T7418	T7416	pobj	Cells,in
R4553	T7420	T7421	amod	Atrxnull,ES
R4554	T7421	T7422	compound	ES,cells
R4555	T7422	T7423	nsubjpass	cells,recovered
R4556	T7424	T7423	aux	could,recovered
R4557	T7425	T7423	neg	not,recovered
R4558	T7426	T7423	auxpass	be,recovered
R4559	T7427	T7423	agent	by,recovered
R456	T942	T941	amod	truncated,isoform
R4560	T7428	T7429	amod	direct,targeting
R4561	T7429	T7427	pobj	targeting,by
R4562	T7430	T7423	cc	and,recovered
R4563	T7431	T7432	auxpass	were,generated
R4564	T7432	T7423	conj	generated,recovered
R4565	T7433	T7432	advmod	eventually,generated
R4566	T7434	T7432	prep	by,generated
R4567	T7435	T7434	pcomp	adopting,by
R4568	T7436	T7437	det	a,approach
R4569	T7437	T7435	dobj	approach,adopting
R457	T943	T941	acl	called,isoform
R4570	T7438	T7439	amod	conditional,targeting
R4571	T7439	T7437	compound	targeting,approach
R4572	T7440	T7423	punct	.,recovered
R4573	T7442	T7443	nsubj	This,is
R4574	T7444	T7443	acomp	consistent,is
R4575	T7445	T7444	prep	with,consistent
R4576	T7446	T7447	poss	our,observation
R4577	T7447	T7445	pobj	observation,with
R4578	T7448	T7449	mark	that,is
R4579	T7449	T7447	advcl	is,observation
R458	T944	T943	oprd	ATRXt,called
R4580	T7450	T7449	nsubj	Atrx,is
R4581	T7451	T7452	advmod	highly,expressed
R4582	T7452	T7449	acomp	expressed,is
R4583	T7453	T7449	prep	in,is
R4584	T7454	T7455	compound	ES,cells
R4585	T7455	T7453	pobj	cells,in
R4586	T7456	T7449	punct	", ",is
R4587	T7457	T7449	cc	and,is
R4588	T7458	T7459	mark	that,imparts
R4589	T7459	T7449	conj	imparts,is
R459	T945	T946	punct	(,kDa
R4590	T7460	T7461	det	the,absence
R4591	T7461	T7459	nsubj	absence,imparts
R4592	T7462	T7461	prep	of,absence
R4593	T7463	T7464	amod	full,length
R4594	T7464	T7466	compound	length,Atrx
R4595	T7465	T7464	punct	-,length
R4596	T7466	T7462	pobj	Atrx,of
R4597	T7467	T7468	det	a,disadvantage
R4598	T7468	T7459	dobj	disadvantage,imparts
R4599	T7469	T7468	compound	growth,disadvantage
R46	T205	T201	dobj	example,provided
R460	T946	T941	parataxis	kDa,isoform
R4600	T7470	T7459	advcl	relative,imparts
R4601	T7471	T7470	prep	to,relative
R4602	T7472	T7471	pobj	cells,to
R4603	T7473	T7472	acl	bearing,cells
R4604	T7474	T7475	det	a,allele
R4605	T7475	T7473	dobj	allele,bearing
R4606	T7476	T7475	amod	functional,allele
R4607	T7477	T7475	compound	Atrx,allele
R4608	T7478	T7443	punct	.,is
R4609	T7480	T7481	prep	At,is
R461	T947	T948	punct	~,200
R4610	T7482	T7480	amod	present,At
R4611	T7483	T7481	punct	", ",is
R4612	T7484	T7485	det	the,cause
R4613	T7485	T7481	nsubj	cause,is
R4614	T7486	T7485	prep	of,cause
R4615	T7487	T7488	det	the,delay
R4616	T7488	T7486	pobj	delay,of
R4617	T7489	T7488	amod	proliferative,delay
R4618	T7490	T7488	prep	in,delay
R4619	T7491	T7492	amod	Atrxnull,cells
R462	T948	T946	nummod	200,kDa
R4620	T7492	T7490	pobj	cells,in
R4621	T7493	T7492	compound	ES,cells
R4622	T7494	T7481	neg	not,is
R4623	T7495	T7481	acomp	known,is
R4624	T7496	T7481	punct	.,is
R4625	T7498	T7499	advmod	Interestingly,demonstrated
R4626	T7500	T7499	punct	", ",demonstrated
R4627	T7501	T7499	nsubj	we,demonstrated
R4628	T7502	T7503	mark	that,regulated
R4629	T7503	T7499	ccomp	regulated,demonstrated
R463	T949	T946	punct	),kDa
R4630	T7504	T7503	nsubjpass	apoptosis,regulated
R4631	T7505	T7503	auxpass	is,regulated
R4632	T7506	T7503	neg	not,regulated
R4633	T7507	T7503	advmod	significantly,regulated
R4634	T7508	T7503	advmod	up,regulated
R4635	T7509	T7503	punct	-,regulated
R4636	T7510	T7503	prep	in,regulated
R4637	T7511	T7512	compound	ES,cells
R4638	T7512	T7510	pobj	cells,in
R4639	T7513	T7512	acl	lacking,cells
R464	T950	T939	auxpass	is,produced
R4640	T7514	T7513	dobj	Atrx,lacking
R4641	T7515	T7503	cc	and,regulated
R4642	T7516	T7503	conj	is,regulated
R4643	T7517	T7518	advmod	only,mildly
R4644	T7518	T7519	advmod	mildly,elevated
R4645	T7519	T7516	acomp	elevated,is
R4646	T7520	T7516	prep	in,is
R4647	T7521	T7522	amod	Atrxnull,embryos
R4648	T7522	T7520	pobj	embryos,in
R4649	T7523	T7524	nummod	7.5,dpc
R465	T951	T939	prep	from,produced
R4650	T7524	T7522	compound	dpc,embryos
R4651	T7525	T7522	compound	mouse,embryos
R4652	T7526	T7499	punct	.,demonstrated
R4653	T7528	T7529	prep	In,shown
R4654	T7530	T7528	pobj	contrast,In
R4655	T7531	T7529	punct	", ",shown
R4656	T7532	T7529	nsubjpass	it,shown
R4657	T7533	T7529	auxpass	was,shown
R4658	T7534	T7529	advmod	recently,shown
R4659	T7535	T7536	mark	that,increased
R466	T952	T953	det	a,transcript
R4660	T7536	T7529	ccomp	increased,shown
R4661	T7537	T7538	det	the,loss
R4662	T7538	T7536	nsubj	loss,increased
R4663	T7539	T7538	prep	of,loss
R4664	T7540	T7539	pobj	Atrx,of
R4665	T7541	T7536	advmod	markedly,increased
R4666	T7542	T7543	det	the,population
R4667	T7543	T7536	dobj	population,increased
R4668	T7544	T7543	amod	apoptotic,population
R4669	T7545	T7536	prep	in,increased
R467	T953	T951	pobj	transcript,from
R4670	T7546	T7547	det	the,cells
R4671	T7547	T7545	pobj	cells,in
R4672	T7548	T7547	amod	differentiating,cells
R4673	T7549	T7547	prep	of,cells
R4674	T7550	T7551	det	the,cortex
R4675	T7551	T7549	pobj	cortex,of
R4676	T7552	T7551	amod	embryonic,cortex
R4677	T7553	T7551	cc	and,cortex
R4678	T7554	T7555	amod	postnatal,hippocampus
R4679	T7555	T7551	conj	hippocampus,cortex
R468	T954	T953	prep	of,transcript
R4680	T7556	T7536	punct	", ",increased
R4681	T7557	T7558	advmod	when,ablated
R4682	T7558	T7536	advcl	ablated,increased
R4683	T7559	T7560	compound	Atrx,expression
R4684	T7560	T7558	nsubjpass	expression,ablated
R4685	T7561	T7558	auxpass	was,ablated
R4686	T7562	T7558	prep	in,ablated
R4687	T7563	T7564	det	the,forebrain
R4688	T7564	T7562	pobj	forebrain,in
R4689	T7565	T7564	amod	developing,forebrain
R469	T955	T956	quantmod	around,7
R4690	T7566	T7564	compound	mouse,forebrain
R4691	T7567	T7536	advcl	using,increased
R4692	T7568	T7569	det	the,allele
R4693	T7569	T7567	dobj	allele,using
R4694	T7570	T7569	compound	Atrx flox,allele
R4695	T7571	T7569	acl	described,allele
R4696	T7572	T7571	advmod	here,described
R4697	T7573	T7574	punct	[,20
R4698	T7574	T7529	parataxis	20,shown
R4699	T7575	T7574	punct	],20
R47	T206	T205	amod	first,example
R470	T956	T957	nummod	7,kb
R4700	T7576	T7529	punct	.,shown
R4701	T7578	T7579	det	The,protein
R4702	T7579	T7582	nsubjpass	protein,shown
R4703	T7580	T7579	amod	human,protein
R4704	T7581	T7579	compound	ATRX,protein
R4705	T7583	T7582	aux	has,shown
R4706	T7584	T7582	auxpass	been,shown
R4707	T7585	T7586	aux	to,associate
R4708	T7586	T7582	xcomp	associate,shown
R4709	T7587	T7586	prep	in,associate
R471	T957	T954	pobj	kb,of
R4710	T7588	T7589	det	a,complex
R4711	T7589	T7587	pobj	complex,in
R4712	T7590	T7586	prep	with,associate
R4713	T7591	T7590	pobj	Daxx,with
R4714	T7592	T7593	punct	[,8
R4715	T7593	T7591	parataxis	8,Daxx
R4716	T7594	T7593	punct	],8
R4717	T7595	T7591	punct	", ",Daxx
R4718	T7596	T7597	det	a,protein
R4719	T7597	T7591	appos	protein,Daxx
R472	T958	T953	punct	", ",transcript
R4720	T7598	T7599	dep	that,implicated
R4721	T7599	T7597	relcl	implicated,protein
R4722	T7600	T7599	aux	has,implicated
R4723	T7601	T7599	auxpass	been,implicated
R4724	T7602	T7599	prep	in,implicated
R4725	T7603	T7604	amod	multiple,pathways
R4726	T7604	T7602	pobj	pathways,in
R4727	T7605	T7599	prep	for,implicated
R4728	T7606	T7607	det	the,regulation
R4729	T7607	T7605	pobj	regulation,for
R473	T959	T960	dep	which,arises
R4730	T7608	T7607	prep	of,regulation
R4731	T7609	T7608	pobj	apoptosis,of
R4732	T7610	T7611	punct	[,21
R4733	T7611	T7582	parataxis	21,shown
R4734	T7612	T7611	punct	],21
R4735	T7613	T7582	punct	.,shown
R4736	T7615	T7616	nsubj	It,is
R4737	T7617	T7616	acomp	possible,is
R4738	T7618	T7619	mark	that,triggered
R4739	T7619	T7616	ccomp	triggered,is
R474	T960	T953	relcl	arises,transcript
R4740	T7620	T7619	nsubj	disruption,triggered
R4741	T7621	T7620	prep	of,disruption
R4742	T7622	T7623	det	the,complex
R4743	T7623	T7621	pobj	complex,of
R4744	T7624	T7623	compound	mouse,complex
R4745	T7625	T7626	compound	Atrx,Daxx
R4746	T7626	T7623	compound	Daxx,complex
R4747	T7627	T7626	punct	-,Daxx
R4748	T7628	T7620	punct	(,disruption
R4749	T7629	T7620	prep	by,disruption
R475	T961	T962	advmod	when,fails
R4750	T7630	T7629	pobj	ablation,by
R4751	T7631	T7630	prep	of,ablation
R4752	T7632	T7633	det	the,protein
R4753	T7633	T7631	pobj	protein,of
R4754	T7634	T7633	compound	Atrx,protein
R4755	T7635	T7619	punct	),triggered
R4756	T7636	T7619	aux	could,triggered
R4757	T7637	T7619	aux	have,triggered
R4758	T7638	T7639	det	a,response
R4759	T7639	T7619	dobj	response,triggered
R476	T962	T960	advcl	fails,arises
R4760	T7640	T7639	amod	universal,response
R4761	T7641	T7639	amod	proapoptotic,response
R4762	T7642	T7616	punct	.,is
R4763	T7644	T7645	advmod	However,demonstrate
R4764	T7646	T7645	punct	", ",demonstrate
R4765	T7647	T7648	poss	our,observations
R4766	T7648	T7645	nsubj	observations,demonstrate
R4767	T7649	T7648	prep	in,observations
R4768	T7650	T7651	compound	ES,cells
R4769	T7651	T7649	pobj	cells,in
R477	T963	T962	nsubj	intron,fails
R4770	T7652	T7653	mark	that,is
R4771	T7653	T7645	ccomp	is,demonstrate
R4772	T7654	T7655	det	the,induction
R4773	T7655	T7653	nsubj	induction,is
R4774	T7656	T7655	prep	of,induction
R4775	T7657	T7656	pobj	apoptosis,of
R4776	T7658	T7653	neg	not,is
R4777	T7659	T7660	det	an,response
R4778	T7660	T7653	attr	response,is
R4779	T7661	T7660	amod	automatic,response
R478	T964	T963	nummod	11,intron
R4780	T7662	T7660	acl	triggered,response
R4781	T7663	T7662	agent	by,triggered
R4782	T7664	T7665	det	the,removal
R4783	T7665	T7663	pobj	removal,by
R4784	T7666	T7665	prep	of,removal
R4785	T7667	T7666	pobj	Atrx,of
R4786	T7668	T7662	prep	in,triggered
R4787	T7669	T7670	det	all,types
R4788	T7670	T7668	pobj	types,in
R4789	T7671	T7670	compound	cell,types
R479	T965	T966	aux	to,spliced
R4790	T7672	T7645	punct	", ",demonstrate
R4791	T7673	T7645	cc	and,demonstrate
R4792	T7674	T7645	conj	suggest,demonstrate
R4793	T7675	T7676	mark	that,reflect
R4794	T7676	T7674	ccomp	reflect,suggest
R4795	T7677	T7678	det	the,apoptosis
R4796	T7678	T7676	nsubj	apoptosis,reflect
R4797	T7679	T7678	amod	inappropriate,apoptosis
R4798	T7680	T7678	acl	observed,apoptosis
R4799	T7681	T7680	prep	in,observed
R48	T207	T205	prep	of,example
R480	T966	T962	xcomp	spliced,fails
R4800	T7682	T7683	det	the,forebrain
R4801	T7683	T7681	pobj	forebrain,in
R4802	T7684	T7685	compound	Atrx,mutant
R4803	T7685	T7683	compound	mutant,forebrain
R4804	T7686	T7685	punct	-,mutant
R4805	T7687	T7676	aux	may,reflect
R4806	T7688	T7689	det	a,requirement
R4807	T7689	T7676	dobj	requirement,reflect
R4808	T7690	T7689	prep	for,requirement
R4809	T7691	T7690	pobj	Atrx,for
R481	T967	T966	auxpass	be,spliced
R4810	T7692	T7689	prep	during,requirement
R4811	T7693	T7694	amod	terminal,differentiation
R4812	T7694	T7692	pobj	differentiation,during
R4813	T7695	T7645	punct	.,demonstrate
R482	T968	T966	prep	from,spliced
R4824	T8323	T8324	det	An,Role
R4825	T8325	T8324	amod	Unexpected,Role
R4826	T8326	T8324	prep	for,Role
R4827	T8327	T8326	pobj	Atrx,for
R4828	T8328	T8324	prep	in,Role
R4829	T8329	T8328	pobj	Development,in
R483	T969	T970	det	the,transcript
R4830	T8330	T8329	prep	of,Development
R4831	T8331	T8332	det	the,Trophoblast
R4832	T8332	T8330	pobj	Trophoblast,of
R4833	T8333	T8332	compound	Mouse,Trophoblast
R4834	T8335	T8336	nsubj	We,show
R4835	T8337	T8338	mark	that,are
R4836	T8338	T8336	ccomp	are,show
R4837	T8339	T8340	amod	Atrxnull,mice
R4838	T8340	T8338	nsubj	mice,are
R4839	T8341	T8340	amod	male,mice
R484	T970	T968	pobj	transcript,from
R4840	T8342	T8338	neg	not,are
R4841	T8343	T8338	acomp	viable,are
R4842	T8344	T8338	cc	and,are
R4843	T8345	T8346	nsubj	embryos,die
R4844	T8346	T8338	conj	die,are
R4845	T8347	T8346	prep	by,die
R4846	T8348	T8349	quantmod	around,9.5
R4847	T8349	T8350	nummod	9.5,dpc
R4848	T8350	T8347	pobj	dpc,by
R4849	T8351	T8336	punct	.,show
R485	T971	T970	amod	primary,transcript
R4850	T8353	T8354	prep	Before,exhibit
R4851	T8355	T8353	pobj	death,Before
R4852	T8356	T8354	punct	", ",exhibit
R4853	T8357	T8358	amod	Atrxnull,embryos
R4854	T8358	T8354	nsubj	embryos,exhibit
R4855	T8359	T8360	det	a,index
R4856	T8360	T8354	dobj	index,exhibit
R4857	T8361	T8362	advmod	markedly,reduced
R4858	T8362	T8360	amod	reduced,index
R4859	T8363	T8360	amod	mitotic,index
R486	T972	T962	cc	and,fails
R4860	T8364	T8354	punct	", ",exhibit
R4861	T8365	T8354	advcl	suggesting,exhibit
R4862	T8366	T8367	det	a,defect
R4863	T8367	T8365	dobj	defect,suggesting
R4864	T8368	T8367	amod	proliferative,defect
R4865	T8369	T8354	punct	.,exhibit
R4866	T8371	T8372	mark	Although,appears
R4867	T8372	T8379	advcl	appears,display
R4868	T8373	T8374	det	the,compartment
R4869	T8374	T8372	nsubj	compartment,appears
R487	T973	T974	det	an,signal
R4870	T8375	T8374	amod	embryonic,compartment
R4871	T8376	T8374	prep	of,compartment
R4872	T8377	T8378	det	the,conceptus
R4873	T8378	T8376	pobj	conceptus,of
R4874	T8380	T8381	advmod	initially,normal
R4875	T8381	T8372	oprd	normal,appears
R4876	T8382	T8379	punct	", ",display
R4877	T8383	T8379	prep	by,display
R4878	T8384	T8385	nummod	7.5,dpc
R4879	T8385	T8383	pobj	dpc,by
R488	T974	T981	nsubjpass	signal,used
R4880	T8386	T8387	amod	Atrxnull,embryos
R4881	T8387	T8379	nsubj	embryos,display
R4882	T8388	T8379	dobj	abnormalities,display
R4883	T8389	T8388	prep	in,abnormalities
R4884	T8390	T8389	pobj	development,in
R4885	T8391	T8390	prep	of,development
R4886	T8392	T8393	det	the,trophoblast
R4887	T8393	T8391	pobj	trophoblast,of
R4888	T8394	T8388	punct	", ",abnormalities
R4889	T8395	T8388	prep	including,abnormalities
R489	T975	T974	amod	alternative,signal
R4890	T8396	T8397	det	a,depletion
R4891	T8397	T8395	pobj	depletion,including
R4892	T8398	T8397	prep	in,depletion
R4893	T8399	T8400	det	the,population
R4894	T8400	T8398	pobj	population,in
R4895	T8401	T8400	prep	of,population
R4896	T8402	T8401	pobj	TGCs,of
R4897	T8403	T8402	acl	surrounding,TGCs
R4898	T8404	T8405	det	the,conceptus
R4899	T8405	T8403	dobj	conceptus,surrounding
R49	T208	T209	det	a,disease
R490	T976	T974	amod	intronic,signal
R4900	T8406	T8397	cc	and,depletion
R4901	T8407	T8408	det	a,reduction
R4902	T8408	T8397	conj	reduction,depletion
R4903	T8409	T8408	prep	in,reduction
R4904	T8410	T8411	det	the,size
R4905	T8411	T8409	pobj	size,in
R4906	T8412	T8411	prep	of,size
R4907	T8413	T8414	det	the,cone
R4908	T8414	T8412	pobj	cone,of
R4909	T8415	T8414	amod	ectoplacental,cone
R491	T977	T974	nmod	poly,signal
R4910	T8416	T8414	punct	", ",cone
R4911	T8417	T8418	dep	which,contains
R4912	T8418	T8414	relcl	contains,cone
R4913	T8419	T8420	det	the,precursors
R4914	T8420	T8418	dobj	precursors,contains
R4915	T8421	T8420	nmod	diploid,precursors
R4916	T8422	T8420	amod	giant,precursors
R4917	T8423	T8420	compound	cell,precursors
R4918	T8424	T8425	punct	[,22
R4919	T8425	T8379	parataxis	22,display
R492	T978	T979	punct	(,A
R4920	T8426	T8425	punct	],22
R4921	T8427	T8379	punct	.,display
R4922	T8429	T8430	nsubj	TGCs,are
R4923	T8431	T8432	advmod	highly,differentiated
R4924	T8432	T8433	amod	differentiated,cells
R4925	T8433	T8430	attr	cells,are
R4926	T8434	T8433	punct	", ",cells
R4927	T8435	T8433	amod	postmitotic,cells
R4928	T8436	T8437	dep	that,form
R4929	T8437	T8433	relcl	form,cells
R493	T979	T974	parataxis	A,signal
R4930	T8438	T8437	advmod	ultimately,form
R4931	T8439	T8440	det	an,layer
R4932	T8440	T8437	dobj	layer,form
R4933	T8441	T8440	amod	epithelial,layer
R4934	T8442	T8437	prep	at,form
R4935	T8443	T8444	det	the,periphery
R4936	T8444	T8442	pobj	periphery,at
R4937	T8445	T8444	prep	of,periphery
R4938	T8446	T8447	det	the,placenta
R4939	T8447	T8445	pobj	placenta,of
R494	T980	T979	punct	),A
R4940	T8448	T8437	punct	", ",form
R4941	T8449	T8450	dep	which,interfaces
R4942	T8450	T8437	ccomp	interfaces,form
R4943	T8451	T8450	prep	with,interfaces
R4944	T8452	T8453	det	the,cells
R4945	T8453	T8451	pobj	cells,with
R4946	T8454	T8453	amod	maternal,cells
R4947	T8455	T8453	prep	of,cells
R4948	T8456	T8457	det	the,decidua
R4949	T8457	T8455	pobj	decidua,of
R495	T981	T962	conj	used,fails
R4950	T8458	T8459	punct	[,23
R4951	T8459	T8430	parataxis	23,are
R4952	T8460	T8459	punct	],23
R4953	T8461	T8430	punct	.,are
R4954	T8463	T8464	det	These,cells
R4955	T8464	T8467	nsubj	cells,are
R4956	T8465	T8466	advmod	highly,invasive
R4957	T8466	T8464	amod	invasive,cells
R4958	T8468	T8467	acomp	important,are
R4959	T8469	T8468	prep	for,important
R496	T982	T981	auxpass	is,used
R4960	T8470	T8469	pcomp	mediating,for
R4961	T8471	T8472	amod	initial,invasion
R4962	T8472	T8470	dobj	invasion,mediating
R4963	T8473	T8472	prep	of,invasion
R4964	T8474	T8475	det	the,tissue
R4965	T8475	T8473	pobj	tissue,of
R4966	T8476	T8475	amod	uterine,tissue
R4967	T8477	T8467	punct	", ",are
R4968	T8478	T8467	cc	but,are
R4969	T8479	T8480	auxpass	are,involved
R497	T983	T984	punct	[,4
R4970	T8480	T8467	conj	involved,are
R4971	T8481	T8480	advmod	also,involved
R4972	T8482	T8480	prep	in,involved
R4973	T8483	T8482	pcomp	remodelling,in
R4974	T8484	T8485	det	the,decidua
R4975	T8485	T8483	dobj	decidua,remodelling
R4976	T8486	T8485	amod	maternal,decidua
R4977	T8487	T8483	prep	after,remodelling
R4978	T8488	T8487	pobj	implantation,after
R4979	T8489	T8482	cc	and,in
R498	T984	T913	parataxis	4,demonstrated
R4980	T8490	T8482	conj	in,in
R4981	T8491	T8490	pcomp	secreting,in
R4982	T8492	T8491	dobj	hormones,secreting
R4983	T8493	T8494	dep	that,regulate
R4984	T8494	T8492	relcl	regulate,hormones
R4985	T8495	T8496	amod	fetal,growth
R4986	T8496	T8494	dobj	growth,regulate
R4987	T8497	T8495	cc	and,fetal
R4988	T8498	T8495	conj	maternal,fetal
R4989	T8499	T8500	punct	[,24
R499	T985	T984	punct	],4
R4990	T8500	T8480	parataxis	24,involved
R4991	T8501	T8500	punct	],24
R4992	T8502	T8467	punct	.,are
R4993	T8504	T8505	mark	Since,appear
R4994	T8505	T8508	advcl	appear,is
R4995	T8506	T8507	amod	Atrxnull,embryos
R4996	T8507	T8505	nsubj	embryos,appear
R4997	T8509	T8510	aux	to,implant
R4998	T8510	T8505	xcomp	implant,appear
R4999	T8511	T8510	advmod	normally,implant
R5	T158	T153	dobj	Development,Affects
R50	T209	T207	pobj	disease,of
R500	T986	T913	punct	.,demonstrated
R5000	T8512	T8508	punct	", ",is
R5001	T8513	T8508	nsubj	lethality,is
R5002	T8514	T8508	acomp	likely,is
R5003	T8515	T8516	aux	to,arise
R5004	T8516	T8514	xcomp	arise,likely
R5005	T8517	T8516	prep	due,arise
R5006	T8518	T8517	pcomp	to,due
R5007	T8519	T8520	det	a,failure
R5008	T8520	T8517	pobj	failure,due
R5009	T8521	T8520	prep	of,failure
R501	T988	T989	det	The,homolog
R5010	T8522	T8523	compound	TGC,function
R5011	T8523	T8521	pobj	function,of
R5012	T8524	T8516	advmod	later,arise
R5013	T8525	T8516	prep	during,arise
R5014	T8526	T8525	pobj	development,during
R5015	T8527	T8508	punct	.,is
R5016	T8529	T8530	amod	Embryonic,lethality
R5017	T8530	T8531	nsubj	lethality,was
R5018	T8532	T8530	prep	in,lethality
R5019	T8533	T8532	pobj	mice,in
R502	T989	T991	nsubjpass	homolog,situated
R5020	T8534	T8530	prep	in,lethality
R5021	T8535	T8536	det	the,absence
R5022	T8536	T8534	pobj	absence,in
R5023	T8537	T8536	prep	of,absence
R5024	T8538	T8537	pobj	Atrx,of
R5025	T8539	T8540	det	a,finding
R5026	T8540	T8531	attr	finding,was
R5027	T8541	T8540	amod	surprising,finding
R5028	T8542	T8531	punct	", ",was
R5029	T8543	T8544	mark	as,been
R503	T990	T989	compound	mouse,homolog
R5030	T8544	T8531	advcl	been,was
R5031	T8545	T8544	expl	there,been
R5032	T8546	T8544	aux	had,been
R5033	T8547	T8548	det	no,suggestion
R5034	T8548	T8544	attr	suggestion,been
R5035	T8549	T8548	prep	of,suggestion
R5036	T8550	T8551	amod	foetal,loss
R5037	T8551	T8549	pobj	loss,of
R5038	T8552	T8544	prep	in,been
R5039	T8553	T8554	det	the,syndrome
R504	T992	T989	prep	of,homolog
R5040	T8554	T8552	pobj	syndrome,in
R5041	T8555	T8554	amod	human,syndrome
R5042	T8556	T8557	compound	ATR,X
R5043	T8557	T8554	compound	X,syndrome
R5044	T8558	T8557	punct	-,X
R5045	T8559	T8531	punct	.,was
R5046	T8561	T8562	nsubj	It,is
R5047	T8563	T8562	acomp	possible,is
R5048	T8564	T8565	mark	that,is
R5049	T8565	T8562	advcl	is,is
R505	T993	T994	det	the,gene
R5050	T8566	T8567	det	the,role
R5051	T8567	T8565	nsubj	role,is
R5052	T8568	T8567	prep	of,role
R5053	T8569	T8568	pobj	Atrx,of
R5054	T8570	T8567	prep	in,role
R5055	T8571	T8572	det	the,trophoblast
R5056	T8572	T8570	pobj	trophoblast,in
R5057	T8573	T8565	acomp	specific,is
R5058	T8574	T8573	prep	to,specific
R5059	T8575	T8574	pobj	mice,to
R506	T994	T992	pobj	gene,of
R5060	T8576	T8565	cc	and,is
R5061	T8577	T8578	mark	that,has
R5062	T8578	T8565	conj	has,is
R5063	T8579	T8578	nsubj	ATRX,has
R5064	T8580	T8581	det	no,role
R5065	T8581	T8578	dobj	role,has
R5066	T8582	T8578	cc	or,has
R5067	T8583	T8578	conj	is,has
R5068	T8584	T8583	acomp	redundant,is
R5069	T8585	T8578	prep	in,has
R507	T995	T994	compound	ATRX,gene
R5070	T8586	T8587	det	the,trophoblast
R5071	T8587	T8585	pobj	trophoblast,in
R5072	T8588	T8587	amod	human,trophoblast
R5073	T8589	T8562	punct	.,is
R5074	T8591	T8592	advmod	Indeed,is
R5075	T8593	T8592	punct	", ",is
R5076	T8594	T8595	det	the,weight
R5077	T8595	T8592	nsubj	weight,is
R5078	T8596	T8595	compound	birth,weight
R5079	T8597	T8595	prep	of,weight
R508	T996	T989	punct	", ",homolog
R5080	T8598	T8597	pobj	babies,of
R5081	T8599	T8598	prep	with,babies
R5082	T8600	T8601	compound	ATR,X
R5083	T8601	T8603	compound	X,syndrome
R5084	T8602	T8601	punct	-,X
R5085	T8603	T8599	pobj	syndrome,with
R5086	T8604	T8592	advmod	usually,is
R5087	T8605	T8592	acomp	normal,is
R5088	T8606	T8592	punct	.,is
R5089	T8608	T8609	det	An,explanation
R509	T997	T989	appos	Atrx,homolog
R5090	T8609	T8611	nsubj	explanation,is
R5091	T8610	T8609	amod	alternative,explanation
R5092	T8612	T8609	prep	for,explanation
R5093	T8613	T8614	det	the,phenotype
R5094	T8614	T8612	pobj	phenotype,for
R5095	T8615	T8616	advmod	unexpectedly,severe
R5096	T8616	T8614	amod	severe,phenotype
R5097	T8617	T8618	nsubj	we,observed
R5098	T8618	T8614	advcl	observed,phenotype
R5099	T8619	T8618	prep	in,observed
R51	T210	T209	amod	human,disease
R510	T998	T991	punct	", ",situated
R5100	T8620	T8619	pobj	mice,in
R5101	T8621	T8622	mark	that,ablates
R5102	T8622	T8611	ccomp	ablates,is
R5103	T8623	T8624	det	the,deletion
R5104	T8624	T8622	nsubj	deletion,ablates
R5105	T8625	T8624	compound	Atrx Δ18Δneo,deletion
R5106	T8626	T8624	acl	generated,deletion
R5107	T8627	T8626	prep	by,generated
R5108	T8628	T8629	compound	Cre,recombination
R5109	T8629	T8627	pobj	recombination,by
R511	T999	T991	auxpass	is,situated
R5110	T8630	T8622	advmod	completely,ablates
R5111	T8631	T8632	amod	full,length
R5112	T8632	T8634	compound	length,protein
R5113	T8633	T8632	punct	-,length
R5114	T8634	T8622	dobj	protein,ablates
R5115	T8635	T8634	compound	Atrx,protein
R5116	T8636	T8637	punct	(,2E
R5117	T8637	T8611	parataxis	2E,is
R5118	T8638	T8637	compound	Figure,2E
R5119	T8639	T8637	punct	),2E
R512	T1000	T991	advmod	also,situated
R5120	T8640	T8611	punct	.,is
R5121	T8642	T8643	prep	In,appear
R5122	T8644	T8642	pobj	contrast,In
R5123	T8645	T8643	punct	", ",appear
R5124	T8646	T8647	det	all,mutations
R5125	T8647	T8643	nsubj	mutations,appear
R5126	T8648	T8649	npadvmod	disease,causing
R5127	T8649	T8647	amod	causing,mutations
R5128	T8650	T8649	punct	-,causing
R5129	T8651	T8647	acl	characterised,mutations
R513	T1001	T991	prep	on,situated
R5130	T8652	T8651	prep	in,characterised
R5131	T8653	T8654	amod	human,pedigrees
R5132	T8654	T8652	pobj	pedigrees,in
R5133	T8655	T8656	compound	ATR,X
R5134	T8656	T8654	compound	X,pedigrees
R5135	T8657	T8656	punct	-,X
R5136	T8658	T8659	aux	to,give
R5137	T8659	T8643	xcomp	give,appear
R5138	T8660	T8659	dobj	rise,give
R5139	T8661	T8659	prep	to,give
R514	T1002	T1003	det	the,chromosome
R5140	T8662	T8663	amod	hypomorphic,alleles
R5141	T8663	T8661	pobj	alleles,to
R5142	T8664	T8643	punct	.,appear
R5143	T8666	T8667	det	Some,protein
R5144	T8667	T8672	nsubjpass	protein,detected
R5145	T8668	T8669	amod	full,length
R5146	T8669	T8667	compound	length,protein
R5147	T8670	T8669	punct	-,length
R5148	T8671	T8667	compound	ATRX,protein
R5149	T8673	T8672	auxpass	is,detected
R515	T1003	T1001	pobj	chromosome,on
R5150	T8674	T8672	prep	in,detected
R5151	T8675	T8674	pobj	cases,in
R5152	T8676	T8675	acl	predicted,cases
R5153	T8677	T8678	aux	to,have
R5154	T8678	T8676	xcomp	have,predicted
R5155	T8679	T8680	compound	truncating,mutations
R5156	T8680	T8678	dobj	mutations,have
R5157	T8681	T8682	punct	(,data
R5158	T8682	T8672	parataxis	data,detected
R5159	T8683	T8682	dep	RJG,data
R516	T1004	T1003	compound	X,chromosome
R5160	T8684	T8682	punct	", ",data
R5161	T8685	T8682	amod	unpublished,data
R5162	T8686	T8682	punct	),data
R5163	T8687	T8672	punct	", ",detected
R5164	T8688	T8672	cc	and,detected
R5165	T8689	T8690	amod	residual,activity
R5166	T8690	T8692	nsubjpass	activity,detected
R5167	T8691	T8690	compound	ATPase,activity
R5168	T8692	T8672	conj	detected,detected
R5169	T8693	T8690	prep	in,activity
R517	T1005	T991	punct	", ",situated
R5170	T8694	T8695	compound	ATRX,immunoprecipitates
R5171	T8695	T8693	pobj	immunoprecipitates,in
R5172	T8696	T8692	aux	can,detected
R5173	T8697	T8692	auxpass	be,detected
R5174	T8698	T8692	prep	in,detected
R5175	T8699	T8700	compound	Epstein,Barr
R5176	T8700	T8702	compound	Barr,virus
R5177	T8701	T8700	punct	-,Barr
R5178	T8702	T8703	npadvmod	virus,transformed
R5179	T8703	T8705	amod	transformed,lymphocytes
R518	T1006	T991	cc	and,situated
R5180	T8704	T8703	punct	-,transformed
R5181	T8705	T8698	pobj	lymphocytes,in
R5182	T8706	T8705	prep	of,lymphocytes
R5183	T8707	T8708	det	all,patients
R5184	T8708	T8706	pobj	patients,of
R5185	T8709	T8708	amod	human,patients
R5186	T8710	T8708	acl	analysed,patients
R5187	T8711	T8710	prep	to,analysed
R5188	T8712	T8711	pobj	date,to
R5189	T8713	T8714	punct	(,A.
R519	T1007	T1008	advmod	also,gives
R5190	T8714	T8692	meta	A.,detected
R5191	T8715	T8714	nmod	Argentaro,A.
R5192	T8716	T8714	cc	and,A.
R5193	T8717	T8714	conj	M.,A.
R5194	T8718	T8717	nmod	Mitson,M.
R5195	T8719	T8717	punct	", ",M.
R5196	T8720	T8721	amod	unpublished,data
R5197	T8721	T8717	conj	data,M.
R5198	T8722	T8721	punct	),data
R5199	T8723	T8692	punct	.,detected
R52	T211	T209	amod	genetic,disease
R520	T1008	T991	conj	gives,situated
R5200	T8725	T8726	det	The,failure
R5201	T8726	T8727	nsubj	failure,suggests
R5202	T8728	T8729	aux	to,observe
R5203	T8729	T8726	acl	observe,failure
R5204	T8730	T8731	det	a,allele
R5205	T8731	T8729	dobj	allele,observe
R5206	T8732	T8733	advmod	truly,null
R5207	T8733	T8731	amod	null,allele
R5208	T8734	T8731	compound	ATRX,allele
R5209	T8735	T8729	prep	among,observe
R521	T1009	T1008	dobj	rise,gives
R5210	T8736	T8737	amod	human,patients
R5211	T8737	T8735	pobj	patients,among
R5212	T8738	T8727	advmod	strongly,suggests
R5213	T8739	T8740	mark	that,is
R5214	T8740	T8727	ccomp	is,suggests
R5215	T8741	T8740	punct	", ",is
R5216	T8742	T8740	prep	as,is
R5217	T8743	T8742	prep	in,as
R5218	T8744	T8745	det	the,mouse
R5219	T8745	T8743	pobj	mouse,in
R522	T1010	T1008	prep	to,gives
R5220	T8746	T8740	punct	", ",is
R5221	T8747	T8748	det	the,absence
R5222	T8748	T8740	nsubj	absence,is
R5223	T8749	T8748	amod	complete,absence
R5224	T8750	T8748	prep	of,absence
R5225	T8751	T8752	compound	ATRX,protein
R5226	T8752	T8750	pobj	protein,of
R5227	T8753	T8740	acomp	incompatible,is
R5228	T8754	T8753	prep	with,incompatible
R5229	T8755	T8756	amod	human,survival
R523	T1011	T1012	amod	full,length
R5230	T8756	T8754	pobj	survival,with
R5231	T8757	T8756	amod	fetal,survival
R5232	T8758	T8727	punct	.,suggests
R5233	T8760	T8761	mark	While,revealed
R5234	T8761	T8765	advcl	revealed,is
R5235	T8762	T8763	det	this,study
R5236	T8763	T8761	nsubj	study,revealed
R5237	T8764	T8761	aux	has,revealed
R5238	T8766	T8767	det	an,role
R5239	T8767	T8761	dobj	role,revealed
R524	T1012	T1014	nmod	length,isoforms
R5240	T8768	T8767	amod	unexpected,role
R5241	T8769	T8767	prep	for,role
R5242	T8770	T8769	pobj	Atrx,for
R5243	T8771	T8767	prep	in,role
R5244	T8772	T8773	det	the,trophectoderm
R5245	T8773	T8771	pobj	trophectoderm,in
R5246	T8774	T8773	amod	murine,trophectoderm
R5247	T8775	T8761	punct	", ",revealed
R5248	T8776	T8761	prep	as,revealed
R5249	T8777	T8778	det	a,result
R525	T1013	T1012	punct	-,length
R5250	T8778	T8776	pobj	result,as
R5251	T8779	T8778	prep	of,result
R5252	T8780	T8781	det	the,lethality
R5253	T8781	T8779	pobj	lethality,of
R5254	T8782	T8781	amod	early,lethality
R5255	T8783	T8781	acl	observed,lethality
R5256	T8784	T8783	prep	in,observed
R5257	T8785	T8786	amod	Atrxnull,embryos
R5258	T8786	T8784	pobj	embryos,in
R5259	T8787	T8765	nsubj	it,is
R526	T1014	T1010	pobj	isoforms,to
R5260	T8788	T8765	neg	not,is
R5261	T8789	T8765	acomp	possible,is
R5262	T8790	T8791	aux	to,rule
R5263	T8791	T8765	xcomp	rule,is
R5264	T8792	T8791	prt	out,rule
R5265	T8793	T8794	amod	other,roles
R5266	T8794	T8791	dobj	roles,rule
R5267	T8795	T8794	prep	for,roles
R5268	T8796	T8795	pobj	Atrx,for
R5269	T8797	T8794	prep	at,roles
R527	T1015	T1016	punct	(,kDa
R5270	T8798	T8799	amod	later,stages
R5271	T8799	T8797	pobj	stages,at
R5272	T8800	T8799	amod	developmental,stages
R5273	T8801	T8794	prep	in,roles
R5274	T8802	T8801	pobj	tissues,in
R5275	T8803	T8802	prep	of,tissues
R5276	T8804	T8805	det	the,embryo
R5277	T8805	T8803	pobj	embryo,of
R5278	T8806	T8805	amod	proper,embryo
R5279	T8807	T8765	punct	.,is
R528	T1016	T1012	parataxis	kDa,length
R5280	T8809	T8810	advmod	Indeed,show
R5281	T8811	T8810	punct	", ",show
R5282	T8812	T8810	nsubj	we,show
R5283	T8813	T8814	mark	that,is
R5284	T8814	T8810	ccomp	is,show
R5285	T8815	T8814	nsubj	Atrx,is
R5286	T8816	T8817	advmod	highly,expressed
R5287	T8817	T8814	acomp	expressed,is
R5288	T8818	T8814	prep	throughout,is
R5289	T8819	T8820	det	the,embryo
R529	T1017	T1016	dep	Atrx,kDa
R5290	T8820	T8818	pobj	embryo,throughout
R5291	T8821	T8820	amod	entire,embryo
R5292	T8822	T8820	amod	developing,embryo
R5293	T8823	T8814	prep	at,is
R5294	T8824	T8825	nummod	7.5,dpc
R5295	T8825	T8823	pobj	dpc,at
R5296	T8826	T8827	punct	(,5B
R5297	T8827	T8814	parataxis	5B,is
R5298	T8828	T8827	compound	Figure,5B
R5299	T8829	T8827	punct	),5B
R53	T212	T209	acl	associated,disease
R530	T1018	T1016	punct	", ",kDa
R5300	T8830	T8810	punct	", ",show
R5301	T8831	T8810	cc	and,show
R5302	T8832	T8833	nsubj	it,is
R5303	T8833	T8810	conj	is,show
R5304	T8834	T8833	acomp	likely,is
R5305	T8835	T8836	mark	that,turn
R5306	T8836	T8833	ccomp	turn,is
R5307	T8837	T8838	compound	Atrx,function
R5308	T8838	T8836	nsubj	function,turn
R5309	T8839	T8836	aux	will,turn
R531	T1019	T1020	punct	~,280
R5310	T8840	T8836	prt	out,turn
R5311	T8841	T8842	aux	to,be
R5312	T8842	T8836	xcomp	be,turn
R5313	T8843	T8842	acomp	important,be
R5314	T8844	T8843	prep	for,important
R5315	T8845	T8846	amod	other,tissues
R5316	T8846	T8844	pobj	tissues,for
R5317	T8847	T8846	amod	differentiating,tissues
R5318	T8848	T8810	punct	.,show
R5319	T8850	T8851	compound	Tetraploid,aggregation
R532	T1020	T1016	nummod	280,kDa
R5320	T8851	T8852	compound	aggregation,experiments
R5321	T8852	T8853	nsubj	experiments,shed
R5322	T8854	T8852	punct	(,experiments
R5323	T8855	T8856	prep	in,rescued
R5324	T8856	T8852	relcl	rescued,experiments
R5325	T8857	T8855	pobj	which,in
R5326	T8858	T8859	compound	mutant,embryos
R5327	T8859	T8856	nsubjpass	embryos,rescued
R5328	T8860	T8856	auxpass	are,rescued
R5329	T8861	T8856	prep	with,rescued
R533	T1021	T1016	punct	),kDa
R5330	T8862	T8863	amod	wild,type
R5331	T8863	T8865	nmod	type,tissues
R5332	T8864	T8863	punct	-,type
R5333	T8865	T8861	pobj	tissues,with
R5334	T8866	T8865	amod	extraembryonic,tissues
R5335	T8867	T8853	punct	),shed
R5336	T8868	T8853	aux	might,shed
R5337	T8869	T8870	amod	more,light
R5338	T8870	T8853	dobj	light,shed
R5339	T8871	T8853	prep	on,shed
R534	T1022	T1012	cc	and,length
R5340	T8872	T8873	det	the,role
R5341	T8873	T8871	pobj	role,on
R5342	T8874	T8873	prep	of,role
R5343	T8875	T8874	pobj	Atrx,of
R5344	T8876	T8873	prep	during,role
R5345	T8877	T8878	amod	later,development
R5346	T8878	T8876	pobj	development,during
R5347	T8879	T8878	compound	mouse,development
R5348	T8880	T8853	punct	", ",shed
R5349	T8881	T8853	cc	but,shed
R535	T1023	T1012	conj	truncated,length
R5350	T8882	T8883	det	these,issues
R5351	T8883	T8884	nsubjpass	issues,dissected
R5352	T8884	T8853	conj	dissected,shed
R5353	T8885	T8884	aux	can,dissected
R5354	T8886	T8884	auxpass	be,dissected
R5355	T8887	T8888	advmod	more,subtly
R5356	T8888	T8884	advmod	subtly,dissected
R5357	T8889	T8884	prep	by,dissected
R5358	T8890	T8889	pcomp	combining,by
R5359	T8891	T8892	det	the,allele
R536	T1024	T1025	punct	(,kDa
R5360	T8892	T8890	dobj	allele,combining
R5361	T8893	T8892	amod	conditional,allele
R5362	T8894	T8892	compound	Atrx flox,allele
R5363	T8895	T8896	dep	that,generated
R5364	T8896	T8892	relcl	generated,allele
R5365	T8897	T8896	nsubj	we,generated
R5366	T8898	T8896	aux	have,generated
R5367	T8899	T8890	prep	with,combining
R5368	T8900	T8901	amod	different,transgenes
R5369	T8901	T8899	pobj	transgenes,with
R537	T1025	T1023	parataxis	kDa,truncated
R5370	T8902	T8903	npadvmod	tissue,specific
R5371	T8903	T8901	amod	specific,transgenes
R5372	T8904	T8903	punct	-,specific
R5373	T8905	T8901	compound	Cre,transgenes
R5374	T8906	T8884	punct	.,dissected
R5375	T8908	T8909	mark	As,mentioned
R5376	T8909	T8910	advcl	mentioned,revealed
R5377	T8911	T8909	advmod	above,mentioned
R5378	T8912	T8910	punct	", ",revealed
R5379	T8913	T8914	det	this,approach
R538	T1026	T1025	dep	Atrxt,kDa
R5380	T8914	T8910	nsubj	approach,revealed
R5381	T8915	T8910	aux	has,revealed
R5382	T8916	T8910	advmod	already,revealed
R5383	T8917	T8918	det	a,role
R5384	T8918	T8910	dobj	role,revealed
R5385	T8919	T8918	amod	critical,role
R5386	T8920	T8918	prep	for,role
R5387	T8921	T8920	pobj	Atrx,for
R5388	T8922	T8918	prep	during,role
R5389	T8923	T8924	amod	neuronal,differentiation
R539	T1027	T1025	punct	", ",kDa
R5390	T8924	T8922	pobj	differentiation,during
R5391	T8925	T8918	prep	in,role
R5392	T8926	T8927	amod	adult,mice
R5393	T8927	T8925	pobj	mice,in
R5394	T8928	T8929	punct	[,20
R5395	T8929	T8910	parataxis	20,revealed
R5396	T8930	T8929	punct	],20
R5397	T8931	T8910	punct	.,revealed
R5398	T8933	T8934	amod	Further,evidence
R5399	T8934	T8935	nsubjpass	evidence,provided
R54	T213	T212	prep	with,associated
R540	T1028	T1029	punct	~,200
R5400	T8936	T8937	mark	that,required
R5401	T8937	T8934	acl	required,evidence
R5402	T8938	T8937	nsubjpass	Atrx,required
R5403	T8939	T8937	auxpass	is,required
R5404	T8940	T8937	advmod	also,required
R5405	T8941	T8937	prep	at,required
R5406	T8942	T8943	amod	later,stages
R5407	T8943	T8941	pobj	stages,at
R5408	T8944	T8943	prep	of,stages
R5409	T8945	T8946	compound	mouse,development
R541	T1029	T1025	nummod	200,kDa
R5410	T8946	T8944	pobj	development,of
R5411	T8947	T8935	auxpass	is,provided
R5412	T8948	T8935	agent	by,provided
R5413	T8949	T8950	det	the,skewing
R5414	T8950	T8948	pobj	skewing,by
R5415	T8951	T8950	amod	observed,skewing
R5416	T8952	T8950	amod	dramatic,skewing
R5417	T8953	T8950	prep	against,skewing
R5418	T8954	T8955	nmod	Atrx,cells
R5419	T8955	T8953	pobj	cells,against
R542	T1030	T1025	punct	),kDa
R5420	T8956	T8954	punct	-,Atrx
R5421	T8957	T8954	amod	negative,Atrx
R5422	T8958	T8950	prep	in,skewing
R5423	T8959	T8960	det	some,tissues
R5424	T8960	T8958	pobj	tissues,in
R5425	T8961	T8960	amod	somatic,tissues
R5426	T8962	T8960	prep	of,tissues
R5427	T8963	T8964	nmod	carrier,mice
R5428	T8964	T8962	pobj	mice,of
R5429	T8965	T8964	amod	female,mice
R543	T1031	T1032	punct	[,5
R5430	T8966	T8964	punct	", ",mice
R5431	T8967	T8968	poss	whose,tissues
R5432	T8968	T8969	dep	tissues,comprise
R5433	T8969	T8964	relcl	comprise,mice
R5434	T8970	T8969	advmod	initially,comprise
R5435	T8971	T8972	det	a,mosaic
R5436	T8972	T8969	dobj	mosaic,comprise
R5437	T8973	T8972	prep	of,mosaic
R5438	T8974	T8975	npadvmod	Atrx,positive
R5439	T8975	T8977	amod	positive,cells
R544	T1032	T1008	parataxis	5,gives
R5440	T8976	T8975	punct	-,positive
R5441	T8977	T8973	pobj	cells,of
R5442	T8978	T8975	cc	and,positive
R5443	T8979	T8980	npadvmod	Atrx,negative
R5444	T8980	T8975	conj	negative,positive
R5445	T8981	T8980	punct	-,negative
R5446	T8982	T8969	prep	as,comprise
R5447	T8983	T8984	det	a,result
R5448	T8984	T8982	pobj	result,as
R5449	T8985	T8984	prep	of,result
R545	T1033	T1032	nummod	4,5
R5450	T8986	T8987	amod	random,inactivation
R5451	T8987	T8985	pobj	inactivation,of
R5452	T8988	T8987	compound	X,inactivation
R5453	T8989	T8987	punct	-,inactivation
R5454	T8990	T8991	punct	(,M.
R5455	T8991	T8935	meta	M.,provided
R5456	T8992	T8991	nmod	Muers,M.
R5457	T8993	T8991	punct	", ",M.
R5458	T8994	T8991	amod	personal,M.
R5459	T8995	T8991	nmod	communication,M.
R546	T1034	T1032	punct	",",5
R5460	T8996	T8991	punct	),M.
R5461	T8997	T8935	punct	.,provided
R5462	T8999	T9000	nsubj	Atrx,joins
R5463	T9001	T9002	det	an,list
R5464	T9002	T9000	dobj	list,joins
R5465	T9003	T9002	amod	expanding,list
R5466	T9004	T9002	prep	of,list
R5467	T9005	T9006	compound	mouse,genes
R5468	T9006	T9004	pobj	genes,of
R5469	T9007	T9008	prep	for,results
R547	T1035	T1032	punct	],5
R5470	T9008	T9002	relcl	results,list
R5471	T9009	T9007	pobj	which,for
R5472	T9010	T9011	amod	targeted,disruption
R5473	T9011	T9008	nsubj	disruption,results
R5474	T9012	T9008	prep	in,results
R5475	T9013	T9014	amod	peri-implantation,lethality
R5476	T9014	T9012	pobj	lethality,in
R5477	T9015	T9008	prep	as,results
R5478	T9016	T9017	det	a,result
R5479	T9017	T9015	pobj	result,as
R548	T1036	T991	punct	.,situated
R5480	T9018	T9017	prep	of,result
R5481	T9019	T9020	nmod	trophoblast,abnormalities
R5482	T9020	T9018	pobj	abnormalities,of
R5483	T9021	T9019	cc	or,trophoblast
R5484	T9022	T9019	conj	placental,trophoblast
R5485	T9023	T9024	punct	(,reviewed
R5486	T9024	T9000	parataxis	reviewed,joins
R5487	T9025	T9024	prep	in,reviewed
R5488	T9026	T9025	punct	[,in
R5489	T9027	T9025	pobj	25,in
R549	T1038	T1039	npadvmod	Disease,causing
R5490	T9028	T9024	punct	],reviewed
R5491	T9029	T9024	punct	),reviewed
R5492	T9030	T9000	punct	.,joins
R5493	T9032	T9033	nsubj	Comparison,provide
R5494	T9034	T9032	prep	with,Comparison
R5495	T9035	T9036	amod	other,phenotypes
R5496	T9036	T9034	pobj	phenotypes,with
R5497	T9037	T9033	aux	might,provide
R5498	T9038	T9039	det	some,insight
R5499	T9039	T9033	dobj	insight,provide
R55	T214	T213	pobj	defects,with
R550	T1039	T1041	amod	causing,mutations
R5500	T9040	T9033	prep	into,provide
R5501	T9041	T9042	det	the,role
R5502	T9042	T9040	pobj	role,into
R5503	T9043	T9042	prep	of,role
R5504	T9044	T9043	pobj	Atrx,of
R5505	T9045	T9042	prep	in,role
R5506	T9046	T9047	compound	trophoblast,development
R5507	T9047	T9045	pobj	development,in
R5508	T9048	T9033	punct	.,provide
R5509	T9050	T9051	compound	Atrx,mutant
R551	T1040	T1039	punct	-,causing
R5510	T9051	T9053	compound	mutant,embryos
R5511	T9052	T9051	punct	-,mutant
R5512	T9053	T9054	nsubj	embryos,progress
R5513	T9055	T9054	advmod	further,progress
R5514	T9056	T9055	prep	than,further
R5515	T9057	T9056	pobj	embryos,than
R5516	T9058	T9057	amod	nullizygous,embryos
R5517	T9059	T9058	prep	for,nullizygous
R5518	T9060	T9059	pobj	factors,for
R5519	T9061	T9060	acl	involved,factors
R552	T1041	T1043	nsubjpass	mutations,clustered
R5520	T9062	T9061	prep	in,involved
R5521	T9063	T9064	det	the,specification
R5522	T9064	T9062	pobj	specification,in
R5523	T9065	T9064	amod	initial,specification
R5524	T9066	T9064	prep	of,specification
R5525	T9067	T9068	compound	trophoblast,cells
R5526	T9068	T9066	pobj	cells,of
R5527	T9069	T9068	compound	stem,cells
R5528	T9070	T9071	punct	(,as
R5529	T9071	T9064	parataxis	as,specification
R553	T1042	T1041	compound	missense,mutations
R5530	T9072	T9071	amod	such,as
R5531	T9073	T9071	pobj	Cdx2,as
R5532	T9074	T9071	punct	),as
R5533	T9075	T9062	cc	or,in
R5534	T9076	T9062	conj	in,in
R5535	T9077	T9078	compound	stem,maintenance
R5536	T9078	T9076	pobj	maintenance,in
R5537	T9079	T9078	compound	cell,maintenance
R5538	T9080	T9078	cc	and,maintenance
R5539	T9081	T9078	conj	proliferation,maintenance
R554	T1044	T1043	auxpass	are,clustered
R5540	T9082	T9083	punct	(,as
R5541	T9083	T9078	parataxis	as,maintenance
R5542	T9084	T9083	amod	such,as
R5543	T9085	T9083	pobj	Eomes,as
R5544	T9086	T9083	punct	),as
R5545	T9087	T9054	punct	.,progress
R5546	T9089	T9090	compound	Cdx2,mutant
R5547	T9090	T9092	compound	mutant,embryos
R5548	T9091	T9090	punct	-,mutant
R5549	T9092	T9093	nsubj	embryos,fail
R555	T1045	T1043	prep	in,clustered
R5550	T9094	T9095	aux	to,implant
R5551	T9095	T9093	xcomp	implant,fail
R5552	T9096	T9093	cc	and,fail
R5553	T9097	T9093	conj	die,fail
R5554	T9098	T9099	quantmod	between,3.5
R5555	T9099	T9100	nummod	3.5,dpc
R5556	T9100	T9097	dobj	dpc,die
R5557	T9101	T9099	cc	and,3.5
R5558	T9102	T9099	conj	5.5,3.5
R5559	T9103	T9104	punct	[,26
R556	T1046	T1047	nummod	two,regions
R5560	T9104	T9093	parataxis	26,fail
R5561	T9105	T9104	punct	],26
R5562	T9106	T9093	punct	", ",fail
R5563	T9107	T9108	mark	while,implant
R5564	T9108	T9093	advcl	implant,fail
R5565	T9109	T9110	compound	Eomes,mutant
R5566	T9110	T9112	compound	mutant,blastocysts
R5567	T9111	T9110	punct	-,mutant
R5568	T9112	T9108	nsubj	blastocysts,implant
R5569	T9113	T9108	prep	into,implant
R557	T1047	T1045	pobj	regions,in
R5570	T9114	T9115	det	the,uterus
R5571	T9115	T9113	pobj	uterus,into
R5572	T9116	T9108	punct	", ",implant
R5573	T9117	T9108	cc	but,implant
R5574	T9118	T9108	conj	arrest,implant
R5575	T9119	T9120	advmod	soon,after
R5576	T9120	T9118	prep	after,arrest
R5577	T9121	T9120	pobj	implantation,after
R5578	T9122	T9118	prep	without,arrest
R5579	T9123	T9122	pcomp	forming,without
R558	T1048	T1047	prep	of,regions
R5580	T9124	T9125	amod	organised,structures
R5581	T9125	T9123	dobj	structures,forming
R5582	T9126	T9125	amod	embryonic,structures
R5583	T9127	T9126	cc	or,embryonic
R5584	T9128	T9126	conj	extraembryonic,embryonic
R5585	T9129	T9130	punct	[,27
R5586	T9130	T9093	parataxis	27,fail
R5587	T9131	T9130	punct	],27
R5588	T9132	T9093	punct	.,fail
R5589	T9134	T9135	prep	In,implant
R559	T1049	T1050	det	the,gene
R5590	T9136	T9134	pobj	contrast,In
R5591	T9137	T9135	punct	", ",implant
R5592	T9138	T9139	compound	Atrx,mutant
R5593	T9139	T9141	compound	mutant,embryos
R5594	T9140	T9139	punct	-,mutant
R5595	T9141	T9135	nsubj	embryos,implant
R5596	T9142	T9135	advmod	successfully,implant
R5597	T9143	T9135	cc	and,implant
R5598	T9144	T9135	conj	establish,implant
R5599	T9145	T9146	amod	organised,structures
R56	T215	T214	prep	in,defects
R560	T1050	T1048	pobj	gene,of
R5600	T9146	T9144	dobj	structures,establish
R5601	T9147	T9146	amod	embryonic,structures
R5602	T9148	T9144	prep	by,establish
R5603	T9149	T9150	nummod	7.5,dpc
R5604	T9150	T9148	pobj	dpc,by
R5605	T9151	T9135	punct	.,implant
R5606	T9153	T9154	det	The,phenotype
R5607	T9154	T9158	nsubj	phenotype,resembles
R5608	T9155	T9156	compound	Atrx,mutant
R5609	T9156	T9154	compound	mutant,phenotype
R561	T1051	T1047	punct	: ,regions
R5610	T9157	T9156	punct	-,mutant
R5611	T9159	T9158	advmod	closely,resembles
R5612	T9160	T9158	dobj	that,resembles
R5613	T9161	T9160	acl	observed,that
R5614	T9162	T9161	prep	in,observed
R5615	T9163	T9162	pobj	mice,in
R5616	T9164	T9163	amod	nullizygous,mice
R5617	T9165	T9164	prep	for,nullizygous
R5618	T9166	T9167	det	the,factor
R5619	T9167	T9165	pobj	factor,for
R562	T1052	T1053	det	a,domain
R5620	T9168	T9167	amod	basic,factor
R5621	T9169	T9170	compound	helix,helix
R5622	T9170	T9167	compound	helix,factor
R5623	T9171	T9170	punct	-,helix
R5624	T9172	T9170	compound	loop,helix
R5625	T9173	T9170	punct	-,helix
R5626	T9174	T9167	compound	transcription,factor
R5627	T9175	T9167	appos	Hand1,factor
R5628	T9176	T9158	punct	.,resembles
R5629	T9178	T9179	compound	Hand1,mutant
R563	T1053	T1047	appos	domain,regions
R5630	T9179	T9181	compound	mutant,conceptuses
R5631	T9180	T9179	punct	-,mutant
R5632	T9181	T9182	nsubj	conceptuses,arrest
R5633	T9183	T9182	prep	at,arrest
R5634	T9184	T9185	quantmod	around,7.5
R5635	T9185	T9186	nummod	7.5,dpc
R5636	T9186	T9183	pobj	dpc,at
R5637	T9187	T9182	cc	and,arrest
R5638	T9188	T9182	conj	display,arrest
R5639	T9189	T9190	det	a,compartment
R564	T1054	T1055	npadvmod	PHD,like
R5640	T9190	T9188	dobj	compartment,display
R5641	T9191	T9190	amod	normal,compartment
R5642	T9192	T9190	amod	embryonic,compartment
R5643	T9193	T9182	punct	", ",arrest
R5644	T9194	T9182	cc	but,arrest
R5645	T9195	T9182	punct	", ",arrest
R5646	T9196	T9197	prep	like,causes
R5647	T9197	T9182	conj	causes,arrest
R5648	T9198	T9199	compound	Atrx,mutant
R5649	T9199	T9201	compound	mutant,embryos
R565	T1055	T1053	amod	like,domain
R5650	T9200	T9199	punct	-,mutant
R5651	T9201	T9196	pobj	embryos,like
R5652	T9202	T9197	punct	", ",causes
R5653	T9203	T9197	nsubj	ablation,causes
R5654	T9204	T9203	prep	of,ablation
R5655	T9205	T9204	pobj	Hand1,of
R5656	T9206	T9207	det	a,reduction
R5657	T9207	T9197	dobj	reduction,causes
R5658	T9208	T9207	prep	in,reduction
R5659	T9209	T9210	det	the,size
R566	T1056	T1055	punct	-,like
R5660	T9210	T9208	pobj	size,in
R5661	T9211	T9210	prep	of,size
R5662	T9212	T9213	det	the,cone
R5663	T9213	T9211	pobj	cone,of
R5664	T9214	T9213	amod	ectoplacental,cone
R5665	T9215	T9210	cc	and,size
R5666	T9216	T9210	conj	density,size
R5667	T9217	T9216	prep	of,density
R5668	T9218	T9217	pobj	TGCs,of
R5669	T9219	T9220	punct	[,28
R567	T1057	T1053	compound	zinc,domain
R5670	T9220	T9197	parataxis	28,causes
R5671	T9221	T9220	punct	],28
R5672	T9222	T9197	punct	.,causes
R5673	T9224	T9225	prep	As,recovered
R5674	T9226	T9224	prep	with,As
R5675	T9227	T9228	compound	Atrx,mutants
R5676	T9228	T9226	pobj	mutants,with
R5677	T9229	T9225	punct	", ",recovered
R5678	T9230	T9231	advmod	only,conceptuses
R5679	T9231	T9225	nsubjpass	conceptuses,recovered
R568	T1058	T1053	compound	finger,domain
R5680	T9232	T9231	amod	arrested,conceptuses
R5681	T9233	T9232	cc	or,arrested
R5682	T9234	T9232	conj	resorbed,arrested
R5683	T9235	T9236	compound	Hand1,mutant
R5684	T9236	T9231	compound	mutant,conceptuses
R5685	T9237	T9236	punct	-,mutant
R5686	T9238	T9225	auxpass	were,recovered
R5687	T9239	T9225	prep	after,recovered
R5688	T9240	T9241	nummod	8.5,dpc
R5689	T9241	T9239	pobj	dpc,after
R569	T1059	T1053	cc	and,domain
R5690	T9242	T9225	punct	.,recovered
R5691	T9244	T9245	advmod	Also,affects
R5692	T9246	T9245	prep	like,affects
R5693	T9247	T9246	pobj	Atrx,like
R5694	T9248	T9245	punct	", ",affects
R5695	T9249	T9245	nsubj	disruption,affects
R5696	T9250	T9249	prep	of,disruption
R5697	T9251	T9250	pobj	Hand1,of
R5698	T9252	T9245	advmod	specifically,affects
R5699	T9253	T9254	amod	secondary,cell
R57	T216	T217	amod	such,proteins
R570	T1060	T1061	det	a,domain
R5700	T9254	T9256	compound	cell,formation
R5701	T9255	T9254	amod	giant,cell
R5702	T9256	T9245	dobj	formation,affects
R5703	T9257	T9245	punct	", ",affects
R5704	T9258	T9245	cc	and,affects
R5705	T9259	T9260	amod	primary,outgrowths
R5706	T9260	T9262	nsubj	outgrowths,appeared
R5707	T9261	T9260	compound	trophoblast,outgrowths
R5708	T9262	T9245	conj	appeared,affects
R5709	T9263	T9260	prep	from,outgrowths
R571	T1061	T1053	conj	domain,domain
R5710	T9264	T9263	pobj	blastocysts,from
R5711	T9265	T9262	oprd	normal,appeared
R5712	T9266	T9262	punct	.,appeared
R5713	T9268	T9269	nsubjpass	Hand1,required
R5714	T9270	T9269	auxpass	is,required
R5715	T9271	T9269	prep	for,required
R5716	T9272	T9273	amod	terminal,differentiation
R5717	T9273	T9271	pobj	differentiation,for
R5718	T9274	T9273	prep	of,differentiation
R5719	T9275	T9276	amod	secondary,TGCs
R572	T1062	T1063	npadvmod	SNF2,like
R5720	T9276	T9274	pobj	TGCs,of
R5721	T9277	T9269	punct	", ",required
R5722	T9278	T9269	cc	and,required
R5723	T9279	T9280	prep	in,arrest
R5724	T9280	T9269	conj	arrest,required
R5725	T9281	T9282	poss	its,absence
R5726	T9282	T9279	pobj	absence,in
R5727	T9283	T9284	compound	trophoblast,cells
R5728	T9284	T9280	nsubj	cells,arrest
R5729	T9285	T9280	prep	at,arrest
R573	T1063	T1061	amod	like,domain
R5730	T9286	T9287	det	a,stage
R5731	T9287	T9285	pobj	stage,at
R5732	T9288	T9287	compound	precursor,stage
R5733	T9289	T9280	prep	in,arrest
R5734	T9290	T9291	det	the,cone
R5735	T9291	T9289	pobj	cone,in
R5736	T9292	T9291	amod	ectoplacental,cone
R5737	T9293	T9294	punct	[,28
R5738	T9294	T9280	parataxis	28,arrest
R5739	T9295	T9294	nummod	17,28
R574	T1064	T1063	punct	-,like
R5740	T9296	T9294	punct	",",28
R5741	T9297	T9294	punct	],28
R5742	T9298	T9280	punct	.,arrest
R5743	T9300	T9301	prep	Given,be
R5744	T9302	T9303	det	the,similarity
R5745	T9303	T9300	pobj	similarity,Given
R5746	T9304	T9303	prep	of,similarity
R5747	T9305	T9306	det	the,phenotypes
R5748	T9306	T9304	pobj	phenotypes,of
R5749	T9307	T9308	nmod	Atrx,mutant
R575	T1065	T1061	compound	ATPase,domain
R5750	T9308	T9306	compound	mutant,phenotypes
R5751	T9309	T9307	punct	-,Atrx
R5752	T9310	T9307	cc	and,Atrx
R5753	T9311	T9307	conj	Hand1,Atrx
R5754	T9312	T9308	punct	-,mutant
R5755	T9313	T9303	cc	and,similarity
R5756	T9314	T9315	det	the,likelihood
R5757	T9315	T9303	conj	likelihood,similarity
R5758	T9316	T9317	mark	that,acts
R5759	T9317	T9315	acl	acts,likelihood
R576	T1066	T1067	punct	(,Figure
R5760	T9318	T9317	nsubj	Atrx,acts
R5761	T9319	T9317	prep	as,acts
R5762	T9320	T9321	det	a,regulator
R5763	T9321	T9319	pobj	regulator,as
R5764	T9322	T9321	amod	transcriptional,regulator
R5765	T9323	T9317	prep	by,acts
R5766	T9324	T9323	pcomp	modifying,by
R5767	T9325	T9326	compound	chromatin,structure
R5768	T9326	T9324	dobj	structure,modifying
R5769	T9327	T9301	punct	", ",be
R577	T1067	T1043	parataxis	Figure,clustered
R5770	T9328	T9301	nsubj	it,be
R5771	T9329	T9301	aux	will,be
R5772	T9330	T9301	prep	of,be
R5773	T9331	T9330	pobj	interest,of
R5774	T9332	T9333	aux	to,determine
R5775	T9333	T9301	xcomp	determine,be
R5776	T9334	T9335	mark	whether,is
R5777	T9335	T9333	advcl	is,determine
R5778	T9336	T9335	nsubj	Atrx,is
R5779	T9337	T9335	npadvmod	itself,is
R578	T1068	T1067	nummod	1,Figure
R5780	T9338	T9339	det	a,regulator
R5781	T9339	T9335	attr	regulator,is
R5782	T9340	T9339	prep	of,regulator
R5783	T9341	T9342	compound	Hand1,expression
R5784	T9342	T9340	pobj	expression,of
R5785	T9343	T9335	punct	", ",is
R5786	T9344	T9335	cc	or,is
R5787	T9345	T9346	advmod	alternatively,acts
R5788	T9346	T9335	conj	acts,is
R5789	T9347	T9346	mark	whether,acts
R579	T1069	T1067	punct	),Figure
R5790	T9348	T9346	nsubj	it,acts
R5791	T9349	T9346	prep	as,acts
R5792	T9350	T9351	det	a,co-regulator
R5793	T9351	T9349	pobj	co-regulator,as
R5794	T9352	T9351	prep	of,co-regulator
R5795	T9353	T9352	pobj	one,of
R5796	T9354	T9353	cc	or,one
R5797	T9355	T9353	conj	more,one
R5798	T9356	T9353	prep	of,one
R5799	T9357	T9358	det	the,targets
R58	T217	T215	pobj	proteins,in
R580	T1070	T1071	punct	[,6
R5800	T9358	T9356	pobj	targets,of
R5801	T9359	T9358	amod	downstream,targets
R5802	T9360	T9358	amod	transcriptional,targets
R5803	T9361	T9358	prep	of,targets
R5804	T9362	T9361	pobj	Hand1,of
R5805	T9363	T9301	punct	.,be
R5806	T9365	T9366	nsubj	It,is
R5807	T9367	T9366	acomp	noteworthy,is
R5808	T9368	T9369	mark	that,observed
R5809	T9369	T9366	ccomp	observed,is
R581	T1071	T1043	parataxis	6,clustered
R5810	T9370	T9369	punct	", ",observed
R5811	T9371	T9369	prep	in,observed
R5812	T9372	T9373	det	the,mice
R5813	T9373	T9371	pobj	mice,in
R5814	T9374	T9375	npadvmod	brain,specific
R5815	T9375	T9373	amod	specific,mice
R5816	T9376	T9375	punct	-,specific
R5817	T9377	T9378	compound	Atrx,knockout
R5818	T9378	T9373	compound	knockout,mice
R5819	T9379	T9369	punct	", ",observed
R582	T1072	T1071	punct	],6
R5820	T9380	T9381	det	the,defect
R5821	T9381	T9369	nsubjpass	defect,observed
R5822	T9382	T9369	auxpass	was,observed
R5823	T9383	T9369	prep	in,observed
R5824	T9384	T9385	advmod	terminally,differentiating
R5825	T9385	T9386	amod	differentiating,neurons
R5826	T9386	T9383	pobj	neurons,in
R5827	T9387	T9388	punct	[,20
R5828	T9388	T9366	parataxis	20,is
R5829	T9389	T9388	punct	],20
R583	T1073	T1043	punct	.,clustered
R5830	T9390	T9366	punct	.,is
R5831	T9392	T9393	det	The,TGCs
R5832	T9393	T9395	nsubj	TGCs,represent
R5833	T9394	T9393	amod	secondary,TGCs
R5834	T9396	T9393	acl	affected,TGCs
R5835	T9397	T9396	prep	in,affected
R5836	T9398	T9399	det	the,knockout
R5837	T9399	T9397	pobj	knockout,in
R5838	T9400	T9399	amod	universal,knockout
R5839	T9401	T9399	compound	Atrx,knockout
R584	T1075	T1076	det	The,motif
R5840	T9402	T9399	acl	reported,knockout
R5841	T9403	T9402	advmod	here,reported
R5842	T9404	T9395	dobj	one,represent
R5843	T9405	T9404	prep	of,one
R5844	T9406	T9407	det	the,tissues
R5845	T9407	T9405	pobj	tissues,of
R5846	T9408	T9407	amod	first,tissues
R5847	T9409	T9410	advmod	terminally,differentiated
R5848	T9410	T9407	amod	differentiated,tissues
R5849	T9411	T9404	prep	in,one
R585	T1076	T1078	nsubjpass	motif,thought
R5850	T9412	T9413	det	the,mouse
R5851	T9413	T9411	pobj	mouse,in
R5852	T9414	T9413	amod	developing,mouse
R5853	T9415	T9395	punct	", ",represent
R5854	T9416	T9395	cc	and,represent
R5855	T9417	T9418	nsubj	this,point
R5856	T9418	T9395	conj	point,represent
R5857	T9419	T9418	aux	may,point
R5858	T9420	T9418	prep	to,point
R5859	T9421	T9422	det	the,requirement
R586	T1077	T1076	amod	former,motif
R5860	T9422	T9420	pobj	requirement,to
R5861	T9423	T9422	prep	for,requirement
R5862	T9424	T9423	pobj	Atrx,for
R5863	T9425	T9422	prep	in,requirement
R5864	T9426	T9427	det	the,expression
R5865	T9427	T9425	pobj	expression,in
R5866	T9428	T9429	amod	high,level
R5867	T9429	T9427	compound	level,expression
R5868	T9430	T9429	punct	-,level
R5869	T9431	T9427	prep	of,expression
R587	T1079	T1078	auxpass	is,thought
R5870	T9432	T9433	det	some,genes
R5871	T9433	T9431	pobj	genes,of
R5872	T9434	T9435	npadvmod	tissue,specific
R5873	T9435	T9433	amod	specific,genes
R5874	T9436	T9435	punct	-,specific
R5875	T9437	T9422	prep	during,requirement
R5876	T9438	T9439	det	the,stages
R5877	T9439	T9437	pobj	stages,during
R5878	T9440	T9439	amod	final,stages
R5879	T9441	T9439	prep	of,stages
R588	T1080	T1081	aux	to,involved
R5880	T9442	T9441	pobj	differentiation,of
R5881	T9443	T9418	punct	.,point
R5882	T9445	T9446	advmod	Interestingly,expressed
R5883	T9447	T9446	punct	", ",expressed
R5884	T9448	T9449	det	the,genes
R5885	T9449	T9446	nsubjpass	genes,expressed
R5886	T9450	T9451	compound	α,globin
R5887	T9451	T9449	compound	globin,genes
R5888	T9452	T9451	punct	-,globin
R5889	T9453	T9449	punct	", ",genes
R589	T1081	T1078	xcomp	involved,thought
R5890	T9454	T9455	det	the,targets
R5891	T9455	T9449	appos	targets,genes
R5892	T9456	T9455	advmod	only,targets
R5893	T9457	T9455	amod	confirmed,targets
R5894	T9458	T9455	amod	transcriptional,targets
R5895	T9459	T9455	prep	of,targets
R5896	T9460	T9459	pobj	regulation,of
R5897	T9461	T9460	prep	by,regulation
R5898	T9462	T9463	amod	human,ATRX
R5899	T9463	T9461	pobj	ATRX,by
R59	T218	T201	punct	.,provided
R590	T1082	T1081	auxpass	be,involved
R5900	T9464	T9446	punct	", ",expressed
R5901	T9465	T9446	auxpass	are,expressed
R5902	T9466	T9446	advmod	also,expressed
R5903	T9467	T9446	advmod	highly,expressed
R5904	T9468	T9469	advmod	specifically,during
R5905	T9469	T9446	prep	during,expressed
R5906	T9470	T9471	amod	terminal,differentiation
R5907	T9471	T9469	pobj	differentiation,during
R5908	T9472	T9446	prep	within,expressed
R5909	T9473	T9474	det	the,lineage
R591	T1083	T1081	prep	in,involved
R5910	T9474	T9472	pobj	lineage,within
R5911	T9475	T9474	amod	erythroid,lineage
R5912	T9476	T9446	punct	.,expressed
R592	T1084	T1085	nmod	protein,protein
R5927	T9932	T9933	nsubj	Atrx,Escapes
R5928	T9934	T9935	amod	Imprinted,Inactivation
R5929	T9935	T9933	dobj	Inactivation,Escapes
R593	T1085	T1087	compound	protein,interactions
R5930	T9936	T9935	compound	X,Inactivation
R5931	T9937	T9935	punct	-,Inactivation
R5932	T9938	T9933	prep	in,Escapes
R5933	T9939	T9940	amod	Extraembryonic,Tissues
R5934	T9940	T9938	pobj	Tissues,in
R5935	T9941	T9940	prep	of,Tissues
R5936	T9942	T9943	nmod	Carrier,Mice
R5937	T9943	T9941	pobj	Mice,of
R5938	T9944	T9943	amod	Female,Mice
R5939	T9946	T9947	det	Another,finding
R594	T1086	T1085	punct	-,protein
R5940	T9947	T9949	nsubj	finding,is
R5941	T9948	T9947	amod	surprising,finding
R5942	T9950	T9947	prep	of,finding
R5943	T9951	T9952	det	this,study
R5944	T9952	T9950	pobj	study,of
R5945	T9953	T9954	mark	that,appears
R5946	T9954	T9949	ccomp	appears,is
R5947	T9955	T9954	punct	", ",appears
R5948	T9956	T9954	prep	in,appears
R5949	T9957	T9958	nmod	carrier,embryos
R595	T1087	T1083	pobj	interactions,in
R5950	T9958	T9956	pobj	embryos,in
R5951	T9959	T9958	amod	female,embryos
R5952	T9960	T9954	punct	", ",appears
R5953	T9961	T9962	det	a,allele
R5954	T9962	T9954	nsubj	allele,appears
R5955	T9963	T9964	advmod	paternally,inherited
R5956	T9964	T9962	amod	inherited,allele
R5957	T9965	T9962	compound	Atrx WT,allele
R5958	T9966	T9967	aux	to,escape
R5959	T9967	T9954	xcomp	escape,appears
R596	T1088	T1087	prep	in,interactions
R5960	T9968	T9969	det	the,process
R5961	T9969	T9967	dobj	process,escape
R5962	T9970	T9969	prep	of,process
R5963	T9971	T9972	amod	imprinted,inactivation
R5964	T9972	T9970	pobj	inactivation,of
R5965	T9973	T9972	compound	X,inactivation
R5966	T9974	T9972	punct	-,inactivation
R5967	T9975	T9969	punct	", ",process
R5968	T9976	T9977	dep	which,silences
R5969	T9977	T9969	relcl	silences,process
R597	T1089	T1088	pobj	chromatin,in
R5970	T9978	T9977	advmod	ordinarily,silences
R5971	T9979	T9980	det	the,chromosome
R5972	T9980	T9977	dobj	chromosome,silences
R5973	T9981	T9980	compound	Xp,chromosome
R5974	T9982	T9977	prep	in,silences
R5975	T9983	T9984	det	the,compartment
R5976	T9984	T9982	pobj	compartment,in
R5977	T9985	T9984	amod	extraembryonic,compartment
R5978	T9986	T9984	prep	of,compartment
R5979	T9987	T9988	amod	female,tissues
R598	T1090	T1091	punct	[,7
R5980	T9988	T9986	pobj	tissues,of
R5981	T9989	T9988	amod	murine,tissues
R5982	T9990	T9991	punct	[,18
R5983	T9991	T9949	parataxis	18,is
R5984	T9992	T9991	punct	],18
R5985	T9993	T9949	punct	.,is
R5986	T9995	T9996	nsubj	Silencing,render
R5987	T9997	T9995	prep	of,Silencing
R5988	T9998	T9999	det	the,allele
R5989	T9999	T9997	pobj	allele,of
R599	T1091	T1078	parataxis	7,thought
R5990	T10000	T9999	compound	Atrx WT,allele
R5991	T10001	T9995	prep	on,Silencing
R5992	T10002	T10001	pobj	Xp,on
R5993	T10003	T9996	aux	should,render
R5994	T10004	T10005	det	these,females
R5995	T10005	T9996	dobj	females,render
R5996	T10006	T9996	oprd	null,render
R5997	T10007	T10006	prep	for,null
R5998	T10008	T10007	pobj	Atrx,for
R5999	T10009	T9996	prep	in,render
R6	T159	T158	cc	and,Development
R60	T220	T221	aux	To,understand
R600	T1092	T1091	punct	],7
R6000	T10010	T10011	det	the,tissues
R6001	T10011	T10009	pobj	tissues,in
R6002	T10012	T10011	amod	extraembryonic,tissues
R6003	T10013	T9996	punct	", ",render
R6004	T10014	T10015	mark	since,carries
R6005	T10015	T9996	advcl	carries,render
R6006	T10016	T10017	det	the,chromosome
R6007	T10017	T10015	nsubj	chromosome,carries
R6008	T10018	T10019	advmod	normally,active
R6009	T10019	T10017	amod	active,chromosome
R601	T1093	T1078	punct	", ",thought
R6010	T10020	T10017	compound	Xm,chromosome
R6011	T10021	T10022	det	the,allele
R6012	T10022	T10015	dobj	allele,carries
R6013	T10023	T10022	compound	Atrx Δ18Δneo,allele
R6014	T10024	T9996	punct	.,render
R6015	T10026	T10027	mark	Although,normal
R6016	T10027	T10030	advcl	normal,developed
R6017	T10028	T10027	neg	not,normal
R6018	T10029	T10027	advmod	phenotypically,normal
R6019	T10031	T10030	punct	", ",developed
R602	T1094	T1078	cc	and,thought
R6020	T10032	T10033	det	some,females
R6021	T10033	T10030	nsubj	females,developed
R6022	T10034	T10033	compound	Atrx,females
R6023	T10035	T10033	compound	carrier,females
R6024	T10036	T10030	prep	to,developed
R6025	T10037	T10036	pobj	term,to
R6026	T10038	T10030	cc	and,developed
R6027	T10039	T10030	conj	went,developed
R6028	T10040	T10039	prt	on,went
R6029	T10041	T10042	aux	to,reproduce
R603	T1095	T1096	det	the,latter
R6030	T10042	T10039	xcomp	reproduce,went
R6031	T10043	T10030	punct	.,developed
R6032	T10045	T10046	advmod	Thus,is
R6033	T10047	T10046	punct	", ",is
R6034	T10048	T10049	det	the,failure
R6035	T10049	T10046	nsubj	failure,is
R6036	T10050	T10051	aux	to,silence
R6037	T10051	T10049	acl	silence,failure
R6038	T10052	T10051	advmod	correctly,silence
R6039	T10053	T10054	det	the,allele
R604	T1096	T1097	nsubj	latter,is
R6040	T10054	T10051	dobj	allele,silence
R6041	T10055	T10056	advmod	paternally,derived
R6042	T10056	T10054	amod	derived,allele
R6043	T10057	T10054	compound	Atrx WT,allele
R6044	T10058	T10054	prep	in,allele
R6045	T10059	T10060	det	the,tissues
R6046	T10060	T10058	pobj	tissues,in
R6047	T10061	T10060	amod	extraembryonic,tissues
R6048	T10062	T10060	prep	of,tissues
R6049	T10063	T10064	compound	carrier,females
R605	T1097	T1078	conj	is,thought
R6050	T10064	T10062	pobj	females,of
R6051	T10065	T10046	acomp	consistent,is
R6052	T10066	T10065	prep	with,consistent
R6053	T10067	T10068	poss	our,observations
R6054	T10068	T10066	pobj	observations,with
R6055	T10069	T10070	mark	that,plays
R6056	T10070	T10068	acl	plays,observations
R6057	T10071	T10070	prep	in,plays
R6058	T10072	T10073	amod	Atrxnull,males
R6059	T10073	T10071	pobj	males,in
R606	T1098	T1099	det	a,feature
R6060	T10074	T10070	punct	", ",plays
R6061	T10075	T10076	det	the,protein
R6062	T10076	T10070	nsubj	protein,plays
R6063	T10077	T10076	compound	Atrx,protein
R6064	T10078	T10079	det	an,role
R6065	T10079	T10070	dobj	role,plays
R6066	T10080	T10079	amod	essential,role
R6067	T10081	T10070	prep	in,plays
R6068	T10082	T10083	det	the,development
R6069	T10083	T10081	pobj	development,in
R607	T1099	T1097	attr	feature,is
R6070	T10084	T10083	prep	of,development
R6071	T10085	T10086	det	the,trophoblast
R6072	T10086	T10084	pobj	trophoblast,of
R6073	T10087	T10070	cc	and,plays
R6074	T10088	T10070	conj	is,plays
R6075	T10089	T10088	acomp	necessary,is
R6076	T10090	T10089	prep	for,necessary
R6077	T10091	T10090	pobj	survival,for
R6078	T10092	T10093	advmod	in,utero
R6079	T10093	T10091	advmod	utero,survival
R608	T1100	T1099	prep	of,feature
R6080	T10094	T10088	prep	in,is
R6081	T10095	T10096	det	the,mouse
R6082	T10096	T10094	pobj	mouse,in
R6083	T10097	T10046	punct	.,is
R6084	T10099	T10100	det	The,survival
R6085	T10100	T10101	nsubj	survival,contrasts
R6086	T10102	T10100	prep	of,survival
R6087	T10103	T10104	compound	Atrx,females
R6088	T10104	T10102	pobj	females,of
R6089	T10105	T10104	compound	carrier,females
R609	T1101	T1102	npadvmod	chromatin,remodelling
R6090	T10106	T10101	prep	with,contrasts
R6091	T10107	T10108	det	the,phenotypes
R6092	T10108	T10106	pobj	phenotypes,with
R6093	T10109	T10108	acl	seen,phenotypes
R6094	T10110	T10109	prep	in,seen
R6095	T10111	T10110	pobj	carriers,in
R6096	T10112	T10111	prep	of,carriers
R6097	T10113	T10112	pobj	mutations,of
R6098	T10114	T10113	prep	of,mutations
R6099	T10115	T10116	amod	other,genes
R61	T221	T223	advcl	understand,inactivated
R610	T1102	T1104	amod	remodelling,proteins
R6100	T10116	T10114	pobj	genes,of
R6101	T10117	T10116	amod	murine,genes
R6102	T10118	T10119	npadvmod	X,linked
R6103	T10119	T10116	amod	linked,genes
R6104	T10120	T10119	punct	-,linked
R6105	T10121	T10116	acl	known,genes
R6106	T10122	T10123	aux	to,be
R6107	T10123	T10121	xcomp	be,known
R6108	T10124	T10123	acomp	essential,be
R6109	T10125	T10123	prep	in,be
R611	T1103	T1102	punct	-,remodelling
R6110	T10126	T10127	det	the,compartment
R6111	T10127	T10125	pobj	compartment,in
R6112	T10128	T10127	amod	extraembryonic,compartment
R6113	T10129	T10101	punct	.,contrasts
R6114	T10131	T10132	prep	For,cause
R6115	T10133	T10131	pobj	example,For
R6116	T10134	T10132	punct	", ",cause
R6117	T10135	T10136	amod	targeted,disruption
R6118	T10136	T10132	nsubj	disruption,cause
R6119	T10137	T10136	prep	of,disruption
R612	T1104	T1100	pobj	proteins,of
R6120	T10138	T10139	det	the,genes
R6121	T10139	T10137	pobj	genes,of
R6122	T10140	T10139	nmod	dyskerin,genes
R6123	T10141	T10140	punct	(,dyskerin
R6124	T10142	T10140	appos	Dkc1,dyskerin
R6125	T10143	T10140	punct	),dyskerin
R6126	T10144	T10140	punct	", ",dyskerin
R6127	T10145	T10146	nmod	glucose,phosphate
R6128	T10146	T10149	compound	phosphate,dehydrogenase
R6129	T10147	T10146	nummod	6,phosphate
R613	T1105	T1078	punct	", ",thought
R6130	T10148	T10146	punct	-,phosphate
R6131	T10149	T10140	conj	dehydrogenase,dyskerin
R6132	T10150	T10149	punct	(,dehydrogenase
R6133	T10151	T10149	appos	G6PD,dehydrogenase
R6134	T10152	T10149	punct	),dehydrogenase
R6135	T10153	T10149	punct	", ",dehydrogenase
R6136	T10154	T10149	cc	and,dehydrogenase
R6137	T10155	T10149	conj	choroideremia,dehydrogenase
R6138	T10156	T10155	punct	(,choroideremia
R6139	T10157	T10155	appos	Chm,choroideremia
R614	T1106	T1078	cc	and,thought
R6140	T10158	T10139	punct	),genes
R6141	T10159	T10160	amod	embryonic,lethality
R6142	T10160	T10132	dobj	lethality,cause
R6143	T10161	T10160	prep	in,lethality
R6144	T10162	T10163	amod	null,embryos
R6145	T10163	T10161	pobj	embryos,in
R6146	T10164	T10163	amod	male,embryos
R6147	T10165	T10132	prep	through,cause
R6148	T10166	T10165	pobj	defects,through
R6149	T10167	T10166	prep	of,defects
R615	T1107	T1108	det	the,presence
R6150	T10168	T10169	det	the,tissues
R6151	T10169	T10167	pobj	tissues,of
R6152	T10170	T10171	amod	extraembryonic,derived
R6153	T10171	T10169	amod	derived,tissues
R6154	T10172	T10171	punct	-,derived
R6155	T10173	T10174	punct	[,29
R6156	T10174	T10132	parataxis	29,cause
R6157	T10175	T10176	punct	–,31
R6158	T10176	T10174	prep	31,29
R6159	T10177	T10174	punct	],29
R616	T1108	T1109	nsubj	presence,indicates
R6160	T10178	T10132	punct	.,cause
R6161	T10180	T10181	amod	Female,mice
R6162	T10181	T10182	nsubj	mice,die
R6163	T10183	T10181	acl	carrying,mice
R6164	T10184	T10183	dobj	mutations,carrying
R6165	T10185	T10184	prep	of,mutations
R6166	T10186	T10187	det	these,genes
R6167	T10187	T10185	pobj	genes,of
R6168	T10188	T10184	prep	on,mutations
R6169	T10189	T10190	det	the,chromosome
R617	T1109	T1078	conj	indicates,thought
R6170	T10190	T10188	pobj	chromosome,on
R6171	T10191	T10192	advmod	maternally,inherited
R6172	T10192	T10190	amod	inherited,chromosome
R6173	T10193	T10190	compound	X,chromosome
R6174	T10194	T10182	advmod	also,die
R6175	T10195	T10196	advmod	in,utero
R6176	T10196	T10182	advmod	utero,die
R6177	T10197	T10182	punct	", ",die
R6178	T10198	T10199	mark	whereas,survive
R6179	T10199	T10182	advcl	survive,die
R618	T1110	T1108	prep	of,presence
R6180	T10200	T10199	nsubj	females,survive
R6181	T10201	T10202	dep	that,inherit
R6182	T10202	T10200	relcl	inherit,females
R6183	T10203	T10204	det	the,mutation
R6184	T10204	T10202	dobj	mutation,inherit
R6185	T10205	T10204	prep	on,mutation
R6186	T10206	T10207	det	the,chromosome
R6187	T10207	T10205	pobj	chromosome,on
R6188	T10208	T10207	compound	Xp,chromosome
R6189	T10209	T10182	punct	.,die
R619	T1111	T1112	npadvmod	disease,causing
R6190	T10211	T10212	advmod	Thus,are
R6191	T10213	T10212	punct	", ",are
R6192	T10214	T10212	prep	unlike,are
R6193	T10215	T10214	pobj	Atrx,unlike
R6194	T10216	T10212	punct	", ",are
R6195	T10217	T10218	det	these,genes
R6196	T10218	T10212	nsubj	genes,are
R6197	T10219	T10218	cc	and,genes
R6198	T10220	T10219	punct	/,and
R6199	T10221	T10219	cc	or,and
R62	T222	T221	advmod	better,understand
R620	T1112	T1114	amod	causing,mutations
R6200	T10222	T10223	poss	their,effects
R6201	T10223	T10218	conj	effects,genes
R6202	T10224	T10223	prep	on,effects
R6203	T10225	T10226	compound	cell,growth
R6204	T10226	T10224	pobj	growth,on
R6205	T10227	T10212	acomp	unable,are
R6206	T10228	T10229	aux	to,circumvent
R6207	T10229	T10227	xcomp	circumvent,unable
R6208	T10230	T10231	det	the,processes
R6209	T10231	T10229	dobj	processes,circumvent
R621	T1113	T1112	punct	-,causing
R6210	T10232	T10233	dep	that,cause
R6211	T10233	T10231	relcl	cause,processes
R6212	T10234	T10233	advmod	ultimately,cause
R6213	T10235	T10236	det	all,cells
R6214	T10236	T10237	nsubj	cells,express
R6215	T10237	T10233	ccomp	express,cause
R6216	T10238	T10236	prep	in,cells
R6217	T10239	T10240	det	the,tissues
R6218	T10240	T10238	pobj	tissues,in
R6219	T10241	T10240	amod	extraembryonic,tissues
R622	T1114	T1110	pobj	mutations,of
R6220	T10242	T10237	aux	to,express
R6221	T10243	T10244	advmod	only,chromosome
R6222	T10244	T10237	dobj	chromosome,express
R6223	T10245	T10244	det	the,chromosome
R6224	T10246	T10247	advmod	maternally,derived
R6225	T10247	T10244	amod	derived,chromosome
R6226	T10248	T10244	compound	X,chromosome
R6227	T10249	T10212	punct	.,are
R6228	T10251	T10252	advmod	How,maintained
R6229	T10253	T10252	aux	might,maintained
R623	T1115	T1116	det	the,importance
R6230	T10254	T10252	nsubjpass	expression,maintained
R6231	T10255	T10254	prep	of,expression
R6232	T10256	T10257	det	the,allele
R6233	T10257	T10255	pobj	allele,of
R6234	T10258	T10259	amod	paternal,Atrx WT
R6235	T10259	T10257	compound	Atrx WT,allele
R6236	T10260	T10252	auxpass	be,maintained
R6237	T10261	T10252	prep	in,maintained
R6238	T10262	T10263	det	the,tissues
R6239	T10263	T10261	pobj	tissues,in
R624	T1116	T1109	dobj	importance,indicates
R6240	T10264	T10263	amod	extraembryonic,tissues
R6241	T10265	T10263	prep	of,tissues
R6242	T10266	T10267	det	the,females
R6243	T10267	T10265	pobj	females,of
R6244	T10268	T10269	compound	Atrx,carrier
R6245	T10269	T10267	compound	carrier,females
R6246	T10270	T10252	punct	?,maintained
R6247	T10272	T10273	nummod	One,possibility
R6248	T10273	T10274	nsubj	possibility,is
R6249	T10275	T10276	mark	that,is
R625	T1117	T1116	amod	functional,importance
R6250	T10276	T10274	ccomp	is,is
R6251	T10277	T10276	punct	", ",is
R6252	T10278	T10276	prep	like,is
R6253	T10279	T10280	det	some,genes
R6254	T10280	T10278	pobj	genes,like
R6255	T10281	T10280	amod	other,genes
R6256	T10282	T10283	npadvmod	X,linked
R6257	T10283	T10280	amod	linked,genes
R6258	T10284	T10283	punct	-,linked
R6259	T10285	T10276	punct	", ",is
R626	T1118	T1116	prep	of,importance
R6260	T10286	T10276	nsubj	silencing,is
R6261	T10287	T10286	prep	of,silencing
R6262	T10288	T10289	det	the,gene
R6263	T10289	T10287	pobj	gene,of
R6264	T10290	T10289	compound	Atrx,gene
R6265	T10291	T10289	prep	on,gene
R6266	T10292	T10291	pobj	Xp,on
R6267	T10293	T10276	acomp	incomplete,is
R6268	T10294	T10276	punct	", ",is
R6269	T10295	T10296	amod	such,is
R627	T1119	T1120	det	these,domains
R6270	T10296	T10276	advcl	is,is
R6271	T10297	T10296	mark	that,is
R6272	T10298	T10296	expl	there,is
R6273	T10299	T10296	advmod	always,is
R6274	T10300	T10301	det	a,output
R6275	T10301	T10296	attr	output,is
R6276	T10302	T10303	amod	low,level
R6277	T10303	T10301	nmod	level,output
R6278	T10304	T10303	punct	-,level
R6279	T10305	T10301	punct	", ",output
R628	T1120	T1118	pobj	domains,of
R6280	T10306	T10301	amod	leaky,output
R6281	T10307	T10301	prep	of,output
R6282	T10308	T10307	pobj	Atrx,of
R6283	T10309	T10301	prep	from,output
R6284	T10310	T10311	det	a,chromosome
R6285	T10311	T10309	pobj	chromosome,from
R6286	T10312	T10313	advmod	normally,inactivated
R6287	T10313	T10311	amod	inactivated,chromosome
R6288	T10314	T10311	compound	Xp,chromosome
R6289	T10315	T10296	prep	in,is
R629	T1121	T1109	punct	.,indicates
R6290	T10316	T10317	amod	extraembryonic,tissues
R6291	T10317	T10315	pobj	tissues,in
R6292	T10318	T10274	punct	.,is
R6293	T10320	T10321	advmod	However,demonstrated
R6294	T10322	T10321	punct	", ",demonstrated
R6295	T10323	T10321	nsubjpass	it,demonstrated
R6296	T10324	T10321	auxpass	was,demonstrated
R6297	T10325	T10321	advmod	recently,demonstrated
R6298	T10326	T10327	mark	that,silenced
R6299	T10327	T10321	ccomp	silenced,demonstrated
R63	T224	T225	det	the,role
R630	T1123	T1124	nsubjpass	ATRX,shown
R6300	T10328	T10329	det	the,allele
R6301	T10329	T10327	nsubjpass	allele,silenced
R6302	T10330	T10331	amod	paternal,Atrx
R6303	T10331	T10329	nmod	Atrx,allele
R6304	T10332	T10331	punct	(,Atrx
R6305	T10333	T10331	acl	called,Atrx
R6306	T10334	T10333	oprd	Xnp,called
R6307	T10335	T10329	punct	),allele
R6308	T10336	T10327	auxpass	is,silenced
R6309	T10337	T10327	advmod	completely,silenced
R631	T1125	T1124	aux	has,shown
R6310	T10338	T10327	prep	in,silenced
R6311	T10339	T10340	det	a,line
R6312	T10340	T10338	pobj	line,in
R6313	T10341	T10340	amod	normal,line
R6314	T10342	T10343	compound	mouse,trophoblast
R6315	T10343	T10340	compound	trophoblast,line
R6316	T10344	T10345	compound	stem,cell
R6317	T10345	T10340	compound	cell,line
R6318	T10346	T10347	punct	[,32
R6319	T10347	T10321	parataxis	32,demonstrated
R632	T1126	T1124	auxpass	been,shown
R6320	T10348	T10347	punct	],32
R6321	T10349	T10321	punct	", ",demonstrated
R6322	T10350	T10321	advcl	suggesting,demonstrated
R6323	T10351	T10352	mark	that,escape
R6324	T10352	T10350	ccomp	escape,suggesting
R6325	T10353	T10352	nsubj	Atrx,escape
R6326	T10354	T10352	aux	does,escape
R6327	T10355	T10352	neg	not,escape
R6328	T10356	T10352	advmod	normally,escape
R6329	T10357	T10358	amod	imprinted,inactivation
R633	T1127	T1128	aux	to,remodel
R6330	T10358	T10352	dobj	inactivation,escape
R6331	T10359	T10358	compound	X,inactivation
R6332	T10360	T10358	punct	-,inactivation
R6333	T10361	T10358	prep	in,inactivation
R6334	T10362	T10363	det	the,tissues
R6335	T10363	T10361	pobj	tissues,in
R6336	T10364	T10363	amod	extraembryonic,tissues
R6337	T10365	T10363	prep	of,tissues
R6338	T10366	T10367	amod	wild,type
R6339	T10367	T10369	compound	type,females
R634	T1128	T1124	xcomp	remodel,shown
R6340	T10368	T10367	punct	-,type
R6341	T10369	T10365	pobj	females,of
R6342	T10370	T10321	punct	.,demonstrated
R6343	T10372	T10373	advmod	Thus,is
R6344	T10374	T10373	punct	", ",is
R6345	T10375	T10376	det	the,expression
R6346	T10376	T10373	nsubj	expression,is
R6347	T10377	T10376	prep	of,expression
R6348	T10378	T10379	det	the,allele
R6349	T10379	T10377	pobj	allele,of
R635	T1129	T1128	dobj	chromatin,remodel
R6350	T10380	T10381	npadvmod	Xp,linked
R6351	T10381	T10379	amod	linked,allele
R6352	T10382	T10381	punct	-,linked
R6353	T10383	T10379	compound	Atrx WT,allele
R6354	T10384	T10385	dep	that,observed
R6355	T10385	T10379	relcl	observed,allele
R6356	T10386	T10385	nsubj	we,observed
R6357	T10387	T10373	acomp	unique,is
R6358	T10388	T10387	prep	to,unique
R6359	T10389	T10390	amod	female,carriers
R636	T1130	T1131	punct	[,8
R6360	T10390	T10388	pobj	carriers,to
R6361	T10391	T10390	prep	of,carriers
R6362	T10392	T10393	det	the,allele
R6363	T10393	T10391	pobj	allele,of
R6364	T10394	T10393	amod	Atrx null,allele
R6365	T10395	T10373	punct	.,is
R6366	T10397	T10398	advmod	Perhaps,stems
R6367	T10399	T10400	det	a,explanation
R6368	T10400	T10398	nsubj	explanation,stems
R6369	T10401	T10402	advmod	more,likely
R637	T1131	T1124	parataxis	8,shown
R6370	T10402	T10400	amod	likely,explanation
R6371	T10403	T10400	prep	for,explanation
R6372	T10404	T10405	det	this,phenomenon
R6373	T10405	T10403	pobj	phenomenon,for
R6374	T10406	T10398	prep	from,stems
R6375	T10407	T10408	amod	experimental,observations
R6376	T10408	T10406	pobj	observations,from
R6377	T10409	T10408	acl	suggesting,observations
R6378	T10410	T10411	mark	that,escape
R6379	T10411	T10409	ccomp	escape,suggesting
R638	T1132	T1131	punct	],8
R6380	T10412	T10413	amod	imprinted,inactivation
R6381	T10413	T10416	nsubjpass	inactivation,imposed
R6382	T10414	T10413	compound	X,inactivation
R6383	T10415	T10413	punct	-,inactivation
R6384	T10416	T10411	ccomp	imposed,escape
R6385	T10417	T10416	auxpass	is,imposed
R6386	T10418	T10416	neg	not,imposed
R6387	T10419	T10416	prep	on,imposed
R6388	T10420	T10421	det	all,precursors
R6389	T10421	T10419	pobj	precursors,on
R639	T1133	T1124	punct	.,shown
R6390	T10422	T10421	prep	of,precursors
R6391	T10423	T10424	det	the,tissues
R6392	T10424	T10422	pobj	tissues,of
R6393	T10425	T10424	nmod	mouse,tissues
R6394	T10426	T10425	amod	extraembryonic,mouse
R6395	T10427	T10411	punct	: ,escape
R6396	T10428	T10429	det	A,subpopulation
R6397	T10429	T10411	nsubj	subpopulation,escape
R6398	T10430	T10429	prep	of,subpopulation
R6399	T10431	T10430	pobj	cells,of
R64	T225	T221	dobj	role,understand
R640	T1135	T1136	nsubj	It,interacts
R6400	T10432	T10411	aux	may,escape
R6401	T10433	T10434	det	this,process
R6402	T10434	T10411	dobj	process,escape
R6403	T10435	T10411	cc	and,escape
R6404	T10436	T10411	conj	make,escape
R6405	T10437	T10438	det	a,choice
R6406	T10438	T10436	dobj	choice,make
R6407	T10439	T10438	amod	random,choice
R6408	T10440	T10438	punct	“,choice
R6409	T10441	T10438	punct	”,choice
R641	T1137	T1136	advmod	also,interacts
R6410	T10442	T10438	prep	of,choice
R6411	T10443	T10444	det	which,chromosome
R6412	T10444	T10446	dep	chromosome,inactivated
R6413	T10445	T10444	compound	X,chromosome
R6414	T10446	T10442	pcomp	inactivated,of
R6415	T10447	T10446	aux	will,inactivated
R6416	T10448	T10446	auxpass	be,inactivated
R6417	T10449	T10398	punct	.,stems
R6418	T10451	T10452	prep	On,expected
R6419	T10453	T10451	amod	average,On
R642	T1138	T1136	prep	with,interacts
R6420	T10454	T10452	punct	", ",expected
R6421	T10455	T10456	nummod	50,%
R6422	T10456	T10452	nsubjpass	%,expected
R6423	T10457	T10456	prep	of,%
R6424	T10458	T10459	det	the,cells
R6425	T10459	T10457	pobj	cells,of
R6426	T10460	T10456	prep	in,%
R6427	T10461	T10462	det	this,subpopulation
R6428	T10462	T10460	pobj	subpopulation,in
R6429	T10463	T10464	advmod	randomly,inactivating
R643	T1139	T1138	pobj	HP1,with
R6430	T10464	T10462	amod	inactivating,subpopulation
R6431	T10465	T10452	aux	would,expected
R6432	T10466	T10452	auxpass	be,expected
R6433	T10467	T10468	aux	to,maintain
R6434	T10468	T10452	xcomp	maintain,expected
R6435	T10469	T10470	det	an,chromosome
R6436	T10470	T10468	dobj	chromosome,maintain
R6437	T10471	T10470	amod	active,chromosome
R6438	T10472	T10470	compound	Xp,chromosome
R6439	T10473	T10452	punct	.,expected
R644	T1140	T1136	prep	at,interacts
R6440	T10475	T10476	prep	In,demonstrated
R6441	T10477	T10475	pobj	support,In
R6442	T10478	T10477	prep	of,support
R6443	T10479	T10480	det	this,hypothesis
R6444	T10480	T10478	pobj	hypothesis,of
R6445	T10481	T10476	punct	", ",demonstrated
R6446	T10482	T10476	nsubjpass	it,demonstrated
R6447	T10483	T10476	aux	has,demonstrated
R6448	T10484	T10476	auxpass	been,demonstrated
R6449	T10485	T10486	mark	that,failed
R645	T1141	T1140	pobj	heterochromatin,at
R6450	T10486	T10476	ccomp	failed,demonstrated
R6451	T10487	T10486	nsubj	expression,failed
R6452	T10488	T10487	prep	of,expression
R6453	T10489	T10490	advmod	paternally,transmitted
R6454	T10490	T10491	amod	transmitted,lacZ
R6455	T10491	T10495	nmod	lacZ,transgenes
R6456	T10492	T10493	npadvmod	X,linked
R6457	T10493	T10491	amod	linked,lacZ
R6458	T10494	T10493	punct	-,linked
R6459	T10495	T10488	pobj	transgenes,of
R646	T1142	T1143	punct	[,9
R6460	T10496	T10497	punct	[,34
R6461	T10497	T10491	parataxis	34,lacZ
R6462	T10498	T10497	nummod	33,34
R6463	T10499	T10497	punct	",",34
R6464	T10500	T10497	punct	],34
R6465	T10501	T10491	cc	and,lacZ
R6466	T10502	T10491	conj	GFP,lacZ
R6467	T10503	T10504	punct	[,35
R6468	T10504	T10502	parataxis	35,GFP
R6469	T10505	T10504	punct	],35
R647	T1143	T1136	parataxis	9,interacts
R6470	T10506	T10507	aux	to,silenced
R6471	T10507	T10486	xcomp	silenced,failed
R6472	T10508	T10507	auxpass	be,silenced
R6473	T10509	T10507	prep	in,silenced
R6474	T10510	T10511	det	a,subpopulation
R6475	T10511	T10509	pobj	subpopulation,in
R6476	T10512	T10511	amod	small,subpopulation
R6477	T10513	T10511	prep	of,subpopulation
R6478	T10514	T10515	amod	extraembryonic,cells
R6479	T10515	T10513	pobj	cells,of
R648	T1144	T1143	punct	],9
R6480	T10516	T10476	punct	.,demonstrated
R6481	T10518	T10519	advmod	Further,shown
R6482	T10520	T10519	punct	", ",shown
R6483	T10521	T10519	nsubjpass	it,shown
R6484	T10522	T10519	aux	has,shown
R6485	T10523	T10519	auxpass	been,shown
R6486	T10524	T10525	mark	that,is
R6487	T10525	T10519	ccomp	is,shown
R6488	T10526	T10525	prep	in,is
R6489	T10527	T10528	det	a,subpopulation
R649	T1145	T1136	cc	and,interacts
R6490	T10528	T10526	pobj	subpopulation,in
R6491	T10529	T10528	prep	of,subpopulation
R6492	T10530	T10531	amod	extraembryonic,cells
R6493	T10531	T10529	pobj	cells,of
R6494	T10532	T10525	punct	", ",is
R6495	T10533	T10525	nsubj	it,is
R6496	T10534	T10535	det	the,Xm
R6497	T10535	T10525	attr	Xm,is
R6498	T10536	T10537	amod	rather,than
R6499	T10537	T10535	cc	than,Xm
R65	T226	T225	prep	of,role
R650	T1146	T1147	auxpass	is,recruited
R6500	T10538	T10539	det	the,Xp
R6501	T10539	T10535	conj	Xp,Xm
R6502	T10540	T10541	dep	that,undergoes
R6503	T10541	T10535	relcl	undergoes,Xm
R6504	T10542	T10543	amod	late,replication
R6505	T10543	T10541	dobj	replication,undergoes
R6506	T10544	T10543	punct	", ",replication
R6507	T10545	T10546	det	a,correlate
R6508	T10546	T10543	appos	correlate,replication
R6509	T10547	T10546	amod	molecular,correlate
R651	T1147	T1136	conj	recruited,interacts
R6510	T10548	T10546	prep	of,correlate
R6511	T10549	T10550	det	the,state
R6512	T10550	T10548	pobj	state,of
R6513	T10551	T10550	amod	inactive,state
R6514	T10552	T10553	punct	[,36
R6515	T10553	T10525	parataxis	36,is
R6516	T10554	T10553	nummod	18,36
R6517	T10555	T10553	punct	",",36
R6518	T10556	T10553	punct	],36
R6519	T10557	T10519	punct	.,shown
R652	T1148	T1147	prep	to,recruited
R6520	T10559	T10560	mark	Although,small
R6521	T10560	T10562	advcl	small,expand
R6522	T10561	T10560	advmod	initially,small
R6523	T10563	T10560	cc	and,small
R6524	T10564	T10565	advmod	quickly,diluted
R6525	T10565	T10560	conj	diluted,small
R6526	T10566	T10565	prep	in,diluted
R6527	T10567	T10568	amod	normal,embryos
R6528	T10568	T10566	pobj	embryos,in
R6529	T10569	T10562	punct	", ",expand
R653	T1149	T1150	amod	promyelocytic,leukemia
R6530	T10570	T10571	det	the,subpopulation
R6531	T10571	T10562	nsubj	subpopulation,expand
R6532	T10572	T10571	amod	cellular,subpopulation
R6533	T10573	T10574	dep	that,inactivates
R6534	T10574	T10571	relcl	inactivates,subpopulation
R6535	T10575	T10576	det	the,chromosome
R6536	T10576	T10574	dobj	chromosome,inactivates
R6537	T10577	T10576	compound	Xm,chromosome
R6538	T10578	T10562	aux	could,expand
R6539	T10579	T10562	advmod	rapidly,expand
R654	T1150	T1151	nmod	leukemia,bodies
R6540	T10580	T10581	aux	to,replace
R6541	T10581	T10562	advcl	replace,expand
R6542	T10582	T10583	det	the,cells
R6543	T10583	T10581	dobj	cells,replace
R6544	T10584	T10585	advmod	normally,imprinted
R6545	T10585	T10583	amod	imprinted,cells
R6546	T10586	T10583	prep	in,cells
R6547	T10587	T10588	amod	extraembryonic,lineages
R6548	T10588	T10586	pobj	lineages,in
R6549	T10589	T10590	mark	if,compromises
R655	T1151	T1148	pobj	bodies,to
R6550	T10590	T10562	advcl	compromises,expand
R6551	T10591	T10592	det	the,silencing
R6552	T10592	T10590	nsubj	silencing,compromises
R6553	T10593	T10592	amod	normal,silencing
R6554	T10594	T10592	prep	of,silencing
R6555	T10595	T10594	pobj	Xp,of
R6556	T10596	T10597	compound	cell,growth
R6557	T10597	T10590	dobj	growth,compromises
R6558	T10598	T10597	cc	or,growth
R6559	T10599	T10597	conj	differentiation,growth
R656	T1152	T1151	amod	nuclear,bodies
R6560	T10600	T10562	punct	.,expand
R6561	T10602	T10603	advmod	Interestingly,suggested
R6562	T10604	T10603	punct	", ",suggested
R6563	T10605	T10603	nsubjpass	it,suggested
R6564	T10606	T10603	aux	has,suggested
R6565	T10607	T10603	auxpass	been,suggested
R6566	T10608	T10609	mark	that,range
R6567	T10609	T10603	ccomp	range,suggested
R6568	T10610	T10611	det	the,size
R6569	T10611	T10609	nsubj	size,range
R657	T1153	T1147	prep	via,recruited
R6570	T10612	T10611	prep	of,size
R6571	T10613	T10614	det	the,population
R6572	T10614	T10612	pobj	population,of
R6573	T10615	T10616	dep	that,escapes
R6574	T10616	T10614	relcl	escapes,population
R6575	T10617	T10616	advmod	initially,escapes
R6576	T10618	T10616	dobj	imprinting,escapes
R6577	T10619	T10609	aux	may,range
R6578	T10620	T10609	advmod	widely,range
R6579	T10621	T10609	punct	(,range
R658	T1154	T1155	det	an,interaction
R6580	T10622	T10609	prep	from,range
R6581	T10623	T10624	nummod	0,%
R6582	T10624	T10622	pobj	%,from
R6583	T10625	T10622	prep	to,from
R6584	T10626	T10627	nummod	30,%
R6585	T10627	T10625	pobj	%,to
R6586	T10628	T10609	punct	),range
R6587	T10629	T10609	punct	", ",range
R6588	T10630	T10631	advmod	even,between
R6589	T10631	T10609	prep	between,range
R659	T1155	T1153	pobj	interaction,via
R6590	T10632	T10633	advmod	genetically,identical
R6591	T10633	T10634	amod	identical,embryos
R6592	T10634	T10631	pobj	embryos,between
R6593	T10635	T10636	punct	[,37
R6594	T10636	T10609	parataxis	37,range
R6595	T10637	T10636	punct	],37
R6596	T10638	T10603	punct	", ",suggested
R6597	T10639	T10603	cc	and,suggested
R6598	T10640	T10641	nsubj	this,account
R6599	T10641	T10603	conj	account,suggested
R66	T227	T226	pobj	ATRX,of
R660	T1156	T1155	prep	with,interaction
R6600	T10642	T10641	aux	may,account
R6601	T10643	T10641	prep	for,account
R6602	T10644	T10645	det	the,phenotype
R6603	T10645	T10643	pobj	phenotype,for
R6604	T10646	T10645	amod	variable,phenotype
R6605	T10647	T10645	acl	observed,phenotype
R6606	T10648	T10647	prep	among,observed
R6607	T10649	T10648	pobj	females,among
R6608	T10650	T10649	acl	bearing,females
R6609	T10651	T10652	npadvmod	Xm,linked
R661	T1157	T1156	pobj	Daxx,with
R6610	T10652	T10654	amod	linked,alleles
R6611	T10653	T10652	punct	-,linked
R6612	T10654	T10650	dobj	alleles,bearing
R6613	T10655	T10654	compound	mutant,alleles
R6614	T10656	T10654	prep	of,alleles
R6615	T10657	T10656	pobj	genes,of
R6616	T10658	T10657	amod	essential,genes
R6617	T10659	T10658	prep	for,essential
R6618	T10660	T10661	amod	normal,development
R6619	T10661	T10659	pobj	development,for
R662	T1158	T1159	punct	[,10
R6620	T10662	T10661	amod	extraembryonic,development
R6621	T10663	T10664	punct	[,38
R6622	T10664	T10641	parataxis	38,account
R6623	T10665	T10664	punct	],38
R6624	T10666	T10641	punct	.,account
R6625	T10668	T10669	advcl	Put,be
R6626	T10670	T10668	advmod	simply,Put
R6627	T10671	T10669	punct	", ",be
R6628	T10672	T10673	compound	carrier,females
R6629	T10673	T10669	nsubj	females,be
R663	T1159	T1147	parataxis	10,recruited
R6630	T10674	T10673	acl	bearing,females
R6631	T10675	T10676	det	a,population
R6632	T10676	T10674	dobj	population,bearing
R6633	T10677	T10676	amod	small,population
R6634	T10678	T10676	amod	initial,population
R6635	T10679	T10676	prep	of,population
R6636	T10680	T10681	amod	escaping,cells
R6637	T10681	T10679	pobj	cells,of
R6638	T10682	T10669	aux	would,be
R6639	T10683	T10684	advmod	more,severely
R664	T1160	T1159	punct	],10
R6640	T10684	T10685	advmod	severely,affected
R6641	T10685	T10669	acomp	affected,be
R6642	T10686	T10685	prep	than,affected
R6643	T10687	T10686	pobj	those,than
R6644	T10688	T10687	acl	bearing,those
R6645	T10689	T10690	det	a,population
R6646	T10690	T10688	dobj	population,bearing
R6647	T10691	T10690	amod	larger,population
R6648	T10692	T10669	punct	.,be
R6649	T10694	T10695	nsubj	This,explain
R665	T1161	T1136	punct	.,interacts
R6650	T10696	T10695	aux	could,explain
R6651	T10697	T10698	advmod	why,observed
R6652	T10698	T10695	ccomp	observed,explain
R6653	T10699	T10698	nsubj	we,observed
R6654	T10700	T10698	aux	have,observed
R6655	T10701	T10702	amod	significant,variation
R6656	T10702	T10698	dobj	variation,observed
R6657	T10703	T10702	amod	phenotypic,variation
R6658	T10704	T10702	prep	among,variation
R6659	T10705	T10706	compound	Atrx,carrier
R666	T1163	T1164	advmod	Furthermore,leads
R6660	T10706	T10707	compound	carrier,females
R6661	T10707	T10704	pobj	females,among
R6662	T10708	T10698	punct	", ",observed
R6663	T10709	T10698	prep	with,observed
R6664	T10710	T10711	det	some,carriers
R6665	T10711	T10712	nsubj	carriers,dying
R6666	T10712	T10709	pobj	dying,with
R6667	T10713	T10714	advmod	in,utero
R6668	T10714	T10712	advmod	utero,dying
R6669	T10715	T10712	prep	by,dying
R667	T1165	T1164	punct	", ",leads
R6670	T10716	T10717	nummod	9.5,dpc
R6671	T10717	T10715	pobj	dpc,by
R6672	T10718	T10719	punct	(,Table
R6673	T10719	T10712	parataxis	Table,dying
R6674	T10720	T10719	nummod	1,Table
R6675	T10721	T10719	punct	),Table
R6676	T10722	T10712	cc	and,dying
R6677	T10723	T10724	nsubj	others,developing
R6678	T10724	T10712	conj	developing,dying
R6679	T10725	T10724	prep	to,developing
R668	T1166	T1164	nsubj	disruption,leads
R6680	T10726	T10725	pobj	term,to
R6681	T10727	T10695	punct	.,explain
R6682	T10729	T10730	det	Another,mechanism
R6683	T10730	T10732	nsubj	mechanism,is
R6684	T10731	T10730	amod	possible,mechanism
R6685	T10733	T10734	mark	that,proceeds
R6686	T10734	T10732	ccomp	proceeds,is
R6687	T10735	T10734	nsubj	inactivation,proceeds
R6688	T10736	T10735	prep	of,inactivation
R6689	T10737	T10738	det	the,X
R669	T1167	T1166	prep	of,disruption
R6690	T10738	T10736	pobj	X,of
R6691	T10739	T10738	amod	paternal,X
R6692	T10740	T10734	advmod	normally,proceeds
R6693	T10741	T10734	prep	in,proceeds
R6694	T10742	T10743	det	all,cells
R6695	T10743	T10741	pobj	cells,in
R6696	T10744	T10734	punct	", ",proceeds
R6697	T10745	T10734	cc	but,proceeds
R6698	T10746	T10747	advmod	subsequently,reactivated
R6699	T10747	T10734	conj	reactivated,proceeds
R67	T228	T225	prep	in,role
R670	T1168	T1167	pobj	ATRX,of
R6700	T10748	T10749	det	the,gene
R6701	T10749	T10747	nsubjpass	gene,reactivated
R6702	T10750	T10749	compound	Atrx,gene
R6703	T10751	T10749	prep	within,gene
R6704	T10752	T10753	amod	individual,cells
R6705	T10753	T10751	pobj	cells,within
R6706	T10754	T10747	auxpass	is,reactivated
R6707	T10755	T10732	punct	.,is
R6708	T10757	T10758	advmod	Alternatively,escape
R6709	T10759	T10758	punct	", ",escape
R671	T1169	T1164	prep	to,leads
R6710	T10760	T10758	prep	in,escape
R6711	T10761	T10762	det	the,absence
R6712	T10762	T10760	pobj	absence,in
R6713	T10763	T10762	prep	of,absence
R6714	T10764	T10763	pobj	Atrx,of
R6715	T10765	T10758	punct	", ",escape
R6716	T10766	T10767	det	the,allele
R6717	T10767	T10758	nsubj	allele,escape
R6718	T10768	T10767	amod	paternal,allele
R6719	T10769	T10758	aux	may,escape
R672	T1170	T1171	amod	diverse,changes
R6720	T10770	T10758	advmod	partially,escape
R6721	T10771	T10772	det	the,process
R6722	T10772	T10758	dobj	process,escape
R6723	T10773	T10772	amod	normal,process
R6724	T10774	T10772	prep	of,process
R6725	T10775	T10774	pobj	silencing,of
R6726	T10776	T10758	punct	.,escape
R6727	T10778	T10779	prep	In,inactivated
R6728	T10780	T10778	pobj	both,In
R6729	T10781	T10780	prep	of,both
R673	T1171	T1169	pobj	changes,to
R6730	T10782	T10783	det	these,cases
R6731	T10783	T10781	pobj	cases,of
R6732	T10784	T10779	punct	", ",inactivated
R6733	T10785	T10786	amod	other,genes
R6734	T10786	T10779	nsubjpass	genes,inactivated
R6735	T10787	T10786	prep	on,genes
R6736	T10788	T10789	det	the,chromosome
R6737	T10789	T10787	pobj	chromosome,on
R6738	T10790	T10789	amod	paternal,chromosome
R6739	T10791	T10789	compound	X,chromosome
R674	T1172	T1171	prep	in,changes
R6740	T10792	T10779	aux	must,inactivated
R6741	T10793	T10779	auxpass	be,inactivated
R6742	T10794	T10779	cc	and,inactivated
R6743	T10795	T10779	conj	remain,inactivated
R6744	T10796	T10795	advmod	so,remain
R6745	T10797	T10779	punct	", ",inactivated
R6746	T10798	T10799	mark	since,causes
R6747	T10799	T10779	advcl	causes,inactivated
R6748	T10800	T10801	amod	blocking,inactivation
R6749	T10801	T10799	nsubj	inactivation,causes
R675	T1173	T1174	compound	DNA,methylation
R6750	T10802	T10801	prep	of,inactivation
R6751	T10803	T10804	det	the,chromosome
R6752	T10804	T10802	pobj	chromosome,of
R6753	T10805	T10804	amod	entire,chromosome
R6754	T10806	T10804	compound	Xp,chromosome
R6755	T10807	T10808	amod	embryonic,lethality
R6756	T10808	T10799	dobj	lethality,causes
R6757	T10809	T10799	prep	due,causes
R6758	T10810	T10809	pcomp	to,due
R6759	T10811	T10812	amod	biallelic,expression
R676	T1174	T1172	pobj	methylation,in
R6760	T10812	T10809	pobj	expression,due
R6761	T10813	T10812	prep	of,expression
R6762	T10814	T10815	npadvmod	X,linked
R6763	T10815	T10817	amod	linked,genes
R6764	T10816	T10815	punct	-,linked
R6765	T10817	T10813	pobj	genes,of
R6766	T10818	T10812	prep	in,expression
R6767	T10819	T10820	det	the,trophoblast
R6768	T10820	T10818	pobj	trophoblast,in
R6769	T10821	T10822	punct	[,39
R677	T1175	T1176	punct	[,11
R6770	T10822	T10779	parataxis	39,inactivated
R6771	T10823	T10822	punct	],39
R6772	T10824	T10779	punct	.,inactivated
R6773	T10927	T10928	compound	ATR,X
R6774	T10928	T10930	compound	X,syndrome
R6775	T10929	T10928	punct	-,X
R6776	T10930	T10931	nsubj	syndrome,is
R6777	T10932	T10933	det	the,disease
R6778	T10933	T10931	attr	disease,is
R6779	T10934	T10933	amod	first,disease
R678	T1176	T1164	parataxis	11,leads
R6780	T10935	T10933	amod	human,disease
R6781	T10936	T10933	amod	genetic,disease
R6782	T10937	T10933	acl	known,disease
R6783	T10938	T10939	aux	to,caused
R6784	T10939	T10937	xcomp	caused,known
R6785	T10940	T10939	auxpass	be,caused
R6786	T10941	T10939	agent	by,caused
R6787	T10942	T10941	pobj	mutations,by
R6788	T10943	T10942	prep	in,mutations
R6789	T10944	T10945	det	a,factor
R679	T1177	T1176	punct	],11
R6790	T10945	T10943	pobj	factor,in
R6791	T10946	T10947	compound	chromatin,remodelling
R6792	T10947	T10945	compound	remodelling,factor
R6793	T10948	T10931	punct	.,is
R6794	T10950	T10951	prep	At,know
R6795	T10952	T10950	amod	present,At
R6796	T10953	T10951	nsubj	we,know
R6797	T10954	T10951	aux	do,know
R6798	T10955	T10951	neg	not,know
R6799	T10956	T10957	advmod	how,influences
R68	T229	T228	pobj	development,in
R680	T1178	T1164	punct	.,leads
R6800	T10957	T10951	advcl	influences,know
R6801	T10958	T10957	nsubj	ATRX,influences
R6802	T10959	T10960	compound	gene,expression
R6803	T10960	T10957	dobj	expression,influences
R6804	T10961	T10957	cc	or,influences
R6805	T10962	T10963	det	what,effect
R6806	T10963	T10964	dep	effect,has
R6807	T10964	T10957	conj	has,influences
R6808	T10965	T10964	nsubj	it,has
R6809	T10966	T10964	prep	on,has
R681	T1180	T1181	advmod	Nevertheless,remains
R6810	T10967	T10968	compound	cell,behaviour
R6811	T10968	T10966	pobj	behaviour,on
R6812	T10969	T10951	punct	.,know
R6813	T10971	T10972	advmod	Nevertheless,noted
R6814	T10973	T10972	punct	", ",noted
R6815	T10974	T10972	nsubj	we,noted
R6816	T10975	T10972	aux	have,noted
R6817	T10976	T10972	advmod	previously,noted
R6818	T10977	T10978	mark	that,are
R6819	T10978	T10972	ccomp	are,noted
R682	T1182	T1181	punct	", ",remains
R6820	T10979	T10978	nsubj	none,are
R6821	T10980	T10979	prep	of,none
R6822	T10981	T10982	det	the,mutations
R6823	T10982	T10980	pobj	mutations,of
R6824	T10983	T10982	amod	natural,mutations
R6825	T10984	T10982	acl	causing,mutations
R6826	T10985	T10986	compound	ATR,X
R6827	T10986	T10988	compound	X,syndrome
R6828	T10987	T10986	punct	-,X
R6829	T10988	T10984	dobj	syndrome,causing
R683	T1183	T1184	det	the,role
R6830	T10989	T10978	attr	nulls,are
R6831	T10990	T10972	punct	", ",noted
R6832	T10991	T10992	dep	which,suggests
R6833	T10992	T10972	advcl	suggests,noted
R6834	T10993	T10994	mark	that,plays
R6835	T10994	T10992	ccomp	plays,suggests
R6836	T10995	T10994	nsubj	it,plays
R6837	T10996	T10997	det	a,role
R6838	T10997	T10994	dobj	role,plays
R6839	T10998	T10997	amod	critical,role
R684	T1184	T1181	nsubj	role,remains
R6840	T10999	T10994	prep	in,plays
R6841	T11000	T11001	amod	normal,development
R6842	T11001	T10999	pobj	development,in
R6843	T11002	T10972	punct	.,noted
R6844	T11004	T11005	nsubj	Results,shows
R6845	T11006	T11004	prep	of,Results
R6846	T11007	T11008	amod	conditional,inactivation
R6847	T11008	T11006	pobj	inactivation,of
R6848	T11009	T11008	prep	of,inactivation
R6849	T11010	T11009	pobj	Atrx,of
R685	T1185	T1186	nsubj	ATRX,plays
R6850	T11011	T11008	prep	in,inactivation
R6851	T11012	T11013	det	the,forebrain
R6852	T11013	T11011	pobj	forebrain,in
R6853	T11014	T11013	amod	developing,forebrain
R6854	T11015	T11013	compound	mouse,forebrain
R6855	T11016	T11004	punct	", ",Results
R6856	T11017	T11004	prep	based,Results
R6857	T11018	T11017	prep	on,based
R6858	T11019	T11020	det	the,allele
R6859	T11020	T11018	pobj	allele,on
R686	T1186	T1184	advcl	plays,role
R6860	T11021	T11020	compound	Atrx flox,allele
R6861	T11022	T11020	acl	described,allele
R6862	T11023	T11022	advmod	here,described
R6863	T11024	T11005	punct	", ",shows
R6864	T11025	T11026	mark	that,exerts
R6865	T11026	T11005	ccomp	exerts,shows
R6866	T11027	T11026	nsubj	Atrx,exerts
R6867	T11028	T11029	det	a,effect
R6868	T11029	T11026	dobj	effect,exerts
R6869	T11030	T11029	amod	major,effect
R687	T1187	T1186	prep	in,plays
R6870	T11031	T11026	prep	on,exerts
R6871	T11032	T11033	advmod	terminally,differentiating
R6872	T11033	T11034	amod	differentiating,neurons
R6873	T11034	T11031	pobj	neurons,on
R6874	T11035	T11005	punct	.,shows
R6875	T11037	T11038	amod	Conditional,inactivation
R6876	T11038	T11039	nsubj	inactivation,is
R6877	T11040	T11038	prep	of,inactivation
R6878	T11041	T11040	pobj	Atrx,of
R6879	T11042	T11038	prep	in,inactivation
R688	T1188	T1189	compound	gene,expression
R6880	T11043	T11044	amod	other,tissues
R6881	T11044	T11042	pobj	tissues,in
R6882	T11045	T11039	acomp	underway,is
R6883	T11046	T11039	punct	.,is
R6884	T11048	T11049	advmod	Here,shown
R6885	T11050	T11049	nsubj	we,shown
R6886	T11051	T11049	aux	have,shown
R6887	T11052	T11053	mark	that,causes
R6888	T11053	T11049	ccomp	causes,shown
R6889	T11054	T11055	npadvmod	animal,wide
R689	T1189	T1187	pobj	expression,in
R6890	T11055	T11057	amod	wide,disruption
R6891	T11056	T11055	punct	-,wide
R6892	T11057	T11053	nsubj	disruption,causes
R6893	T11058	T11057	prep	of,disruption
R6894	T11059	T11060	det	the,gene
R6895	T11060	T11058	pobj	gene,of
R6896	T11061	T11060	compound	Atrx,gene
R6897	T11062	T11063	det	a,phenotype
R6898	T11063	T11053	dobj	phenotype,causes
R6899	T11064	T11063	amod	severe,phenotype
R69	T230	T225	cc	and,role
R690	T1190	T1181	acomp	unclear,remains
R6900	T11065	T11066	amod	embryonic,lethal
R6901	T11066	T11063	amod	lethal,phenotype
R6902	T11067	T11066	punct	-,lethal
R6903	T11068	T11049	punct	", ",shown
R6904	T11069	T11049	advcl	revealing,shown
R6905	T11070	T11071	det	an,role
R6906	T11071	T11069	dobj	role,revealing
R6907	T11072	T11071	amod	essential,role
R6908	T11073	T11071	prep	for,role
R6909	T11074	T11073	pobj	Atrx,for
R691	T1191	T1181	punct	.,remains
R6910	T11075	T11071	prep	in,role
R6911	T11076	T11077	det	the,formation
R6912	T11077	T11075	pobj	formation,in
R6913	T11078	T11077	prep	of,formation
R6914	T11079	T11080	det	the,trophoblast
R6915	T11080	T11078	pobj	trophoblast,of
R6916	T11081	T11080	amod	murine,trophoblast
R6917	T11082	T11049	punct	.,shown
R6918	T11084	T11085	prep	In,appears
R6919	T11086	T11084	pobj	addition,In
R692	T1193	T1194	det	The,core
R6920	T11087	T11085	punct	", ",appears
R6921	T11088	T11085	nsubj	Atrx,appears
R6922	T11089	T11090	aux	to,escape
R6923	T11090	T11085	xcomp	escape,appears
R6924	T11091	T11092	amod	imprinted,chromosome
R6925	T11092	T11095	compound	chromosome,inactivation
R6926	T11093	T11092	compound	X,chromosome
R6927	T11094	T11092	punct	-,chromosome
R6928	T11095	T11090	dobj	inactivation,escape
R6929	T11096	T11090	prep	in,escape
R693	T1194	T1196	nsubj	core,suggests
R6930	T11097	T11098	det	the,tissues
R6931	T11098	T11096	pobj	tissues,in
R6932	T11099	T11098	amod	extraembryonic,tissues
R6933	T11100	T11098	prep	of,tissues
R6934	T11101	T11102	det	some,mice
R6935	T11102	T11100	pobj	mice,of
R6936	T11103	T11102	nmod	carrier,mice
R6937	T11104	T11103	amod	female,carrier
R6938	T11105	T11085	punct	.,appears
R694	T1195	T1194	amod	consistent,core
R6941	T11190	T11189	prep	of,Generation
R6942	T11191	T11192	compound	ES,cells
R6943	T11192	T11190	pobj	cells,of
R6944	T11193	T11192	acl	bearing,cells
R6945	T11194	T11195	det	the,allele
R6946	T11195	T11193	dobj	allele,bearing
R6947	T11196	T11195	compound	Atrx flox,allele
R6948	T11197	T11189	punct	.,Generation
R6949	T11199	T11200	advmod	Briefly,places
R695	T1197	T1194	prep	of,core
R6950	T11201	T11200	punct	", ",places
R6951	T11202	T11203	det	the,vector
R6952	T11203	T11200	nsubj	vector,places
R6953	T11204	T11203	compound	targeting,vector
R6954	T11205	T11203	punct	(,vector
R6955	T11206	T11203	acl	shown,vector
R6956	T11207	T11206	prep	in,shown
R6957	T11208	T11209	compound	Figure,2A
R6958	T11209	T11207	pobj	2A,in
R6959	T11210	T11200	punct	),places
R696	T1198	T1199	amod	clinical,features
R6960	T11211	T11212	det	a,site
R6961	T11212	T11200	dobj	site,places
R6962	T11213	T11212	compound	loxP,site
R6963	T11214	T11200	prep	within,places
R6964	T11215	T11214	pobj	intron,within
R6965	T11216	T11215	nummod	18,intron
R6966	T11217	T11200	cc	and,places
R6967	T11218	T11219	det	a,cassette
R6968	T11219	T11200	conj	cassette,places
R6969	T11220	T11221	npadvmod	loxP,flanked
R697	T1199	T1197	pobj	features,of
R6970	T11221	T11219	amod	flanked,cassette
R6971	T11222	T11221	punct	-,flanked
R6972	T11223	T11224	compound	MC1neopA,selection
R6973	T11224	T11219	compound	selection,cassette
R6974	T11225	T11219	prep	in,cassette
R6975	T11226	T11225	pobj	intron,in
R6976	T11227	T11226	nummod	17,intron
R6977	T11228	T11226	prep	of,intron
R6978	T11229	T11230	det	the,gene
R6979	T11230	T11228	pobj	gene,of
R698	T1200	T1198	cc	and,clinical
R6980	T11231	T11230	compound	Atrx,gene
R6981	T11232	T11200	punct	.,places
R6982	T11234	T11235	det	A,description
R6983	T11235	T11237	nsubjpass	description,provided
R6984	T11236	T11235	amod	detailed,description
R6985	T11238	T11235	prep	of,description
R6986	T11239	T11240	det	the,construct
R6987	T11240	T11238	pobj	construct,of
R6988	T11241	T11240	compound	targeting,construct
R6989	T11242	T11237	auxpass	is,provided
R699	T1201	T1198	conj	haematological,clinical
R6990	T11243	T11237	prep	in,provided
R6991	T11244	T11243	punct	[,in
R6992	T11245	T11243	pobj	20,in
R6993	T11246	T11237	punct	],provided
R6994	T11247	T11237	punct	.,provided
R6995	T11249	T11250	amod	Linearised,plasmid
R6996	T11250	T11251	nsubjpass	plasmid,electroporated
R6997	T11252	T11253	punct	(,μg
R6998	T11253	T11250	parataxis	μg,plasmid
R6999	T11254	T11253	nummod	150,μg
R7	T161	T158	conj	Pattern,Development
R70	T231	T232	det	the,abnormalities
R700	T1202	T1194	acl	observed,core
R7000	T11255	T11253	punct	),μg
R7001	T11256	T11251	auxpass	was,electroporated
R7002	T11257	T11251	prep	into,electroporated
R7003	T11258	T11259	quantmod	1,108
R7004	T11259	T11261	nummod	108,cells
R7005	T11260	T11259	punct	×,108
R7006	T11261	T11257	pobj	cells,into
R7007	T11262	T11261	compound	E14Tg2a,cells
R7008	T11263	T11261	compound	ES,cells
R7009	T11264	T11251	punct	", ",electroporated
R701	T1203	T1202	prep	in,observed
R7010	T11265	T11251	cc	and,electroporated
R7011	T11266	T11267	nsubjpass	colonies,isolated
R7012	T11267	T11251	conj	isolated,electroporated
R7013	T11268	T11266	amod	resistant,colonies
R7014	T11269	T11268	prep	to,resistant
R7015	T11270	T11269	pobj	G418,to
R7016	T11271	T11270	cc	and,G418
R7017	T11272	T11270	conj	ganciclovir,G418
R7018	T11273	T11267	auxpass	were,isolated
R7019	T11274	T11267	punct	.,isolated
R702	T1204	T1205	compound	ATR,X
R7020	T11276	T11277	amod	Homologous,events
R7021	T11277	T11279	nsubjpass	events,identified
R7022	T11278	T11277	compound	targeting,events
R7023	T11280	T11279	auxpass	were,identified
R7024	T11281	T11279	prep	by,identified
R7025	T11282	T11283	compound	Southern,blot
R7026	T11283	T11281	pobj	blot,by
R7027	T11284	T11283	prep	of,blot
R7028	T11285	T11286	npadvmod	EcoRI,digested
R7029	T11286	T11288	amod	digested,DNA
R703	T1205	T1207	compound	X,patients
R7030	T11287	T11286	punct	-,digested
R7031	T11288	T11284	pobj	DNA,of
R7032	T11289	T11283	cc	and,blot
R7033	T11290	T11283	conj	hybridisation,blot
R7034	T11291	T11290	prep	with,hybridisation
R7035	T11292	T11293	det	a,probe
R7036	T11293	T11291	pobj	probe,with
R7037	T11294	T11293	nummod	5,probe
R7038	T11295	T11294	punct	′,5
R7039	T11296	T11293	punct	(,probe
R704	T1206	T1205	punct	-,X
R7040	T11297	T11293	acl	generated,probe
R7041	T11298	T11297	prep	with,generated
R7042	T11299	T11300	compound	primers,PPS1.20
R7043	T11300	T11298	pobj	PPS1.20,with
R7044	T11301	T11300	cc	and,PPS1.20
R7045	T11302	T11300	conj	PPS1.27,PPS1.20
R7046	T11303	T11293	punct	),probe
R7047	T11304	T11293	cc	and,probe
R7048	T11305	T11306	det	a,probe
R7049	T11306	T11293	conj	probe,probe
R705	T1207	T1203	pobj	patients,in
R7050	T11307	T11306	nummod	3,probe
R7051	T11308	T11307	punct	′,3
R7052	T11309	T11306	punct	(,probe
R7053	T11310	T11311	det	a,fragment
R7054	T11311	T11306	appos	fragment,probe
R7055	T11312	T11313	nummod	0.9,kb
R7056	T11313	T11311	compound	kb,fragment
R7057	T11314	T11313	punct	-,kb
R7058	T11315	T11311	compound	HaeIII,fragment
R7059	T11316	T11279	punct	),identified
R706	T1208	T1209	mark	that,regulates
R7060	T11317	T11318	mark	as,shown
R7061	T11318	T11279	advcl	shown,identified
R7062	T11319	T11318	prep	in,shown
R7063	T11320	T11321	compound	Figure,2A
R7064	T11321	T11319	pobj	2A,in
R7065	T11322	T11321	cc	and,2A
R7066	T11323	T11321	conj	2B,2A
R7067	T11324	T11279	punct	.,identified
R7068	T11326	T11327	nsubj	DNA,digested
R7069	T11328	T11326	prep	from,DNA
R707	T1209	T1196	ccomp	regulates,suggests
R7070	T11329	T11330	advmod	correctly,targeted
R7071	T11330	T11331	amod	targeted,clones
R7072	T11331	T11328	pobj	clones,from
R7073	T11332	T11327	aux	was,digested
R7074	T11333	T11327	advmod	also,digested
R7075	T11334	T11327	prep	with,digested
R7076	T11335	T11334	pobj	SacI,with
R7077	T11336	T11327	cc	and,digested
R7078	T11337	T11327	conj	analysed,digested
R7079	T11338	T11337	prep	by,analysed
R708	T1210	T1209	punct	", ",regulates
R7080	T11339	T11340	compound	Southern,blot
R7081	T11340	T11338	pobj	blot,by
R7082	T11341	T11340	cc	and,blot
R7083	T11342	T11340	conj	hybridisation,blot
R7084	T11343	T11342	prep	with,hybridisation
R7085	T11344	T11345	det	a,probe
R7086	T11345	T11343	pobj	probe,with
R7087	T11346	T11345	prep	from,probe
R7088	T11347	T11346	prep	within,from
R7089	T11348	T11347	pobj	intron,within
R709	T1211	T1209	prep	like,regulates
R7090	T11349	T11348	nummod	17,intron
R7091	T11350	T11345	punct	(,probe
R7092	T11351	T11352	det	a,product
R7093	T11352	T11345	appos	product,probe
R7094	T11353	T11352	compound	PCR,product
R7095	T11354	T11352	acl	generated,product
R7096	T11355	T11354	prep	with,generated
R7097	T11356	T11357	compound	primers,PPS1.15
R7098	T11357	T11355	pobj	PPS1.15,with
R7099	T11358	T11357	cc	and,PPS1.15
R71	T232	T225	conj	abnormalities,role
R710	T1212	T1213	det	the,protein
R7100	T11359	T11357	conj	Xnp46,PPS1.15
R7101	T11360	T11327	punct	),digested
R7102	T11361	T11362	aux	to,confirm
R7103	T11362	T11327	advcl	confirm,digested
R7104	T11363	T11364	mark	that,included
R7105	T11364	T11362	ccomp	included,confirm
R7106	T11365	T11366	det	the,site
R7107	T11366	T11364	nsubjpass	site,included
R7108	T11367	T11366	compound	loxP,site
R7109	T11368	T11366	prep	within,site
R711	T1213	T1211	pobj	protein,like
R7110	T11369	T11368	pobj	intron,within
R7111	T11370	T11369	nummod	18,intron
R7112	T11371	T11364	aux	had,included
R7113	T11372	T11364	auxpass	been,included
R7114	T11373	T11364	prep	within,included
R7115	T11374	T11375	det	the,region
R7116	T11375	T11373	pobj	region,within
R7117	T11376	T11375	amod	crossed,region
R7118	T11377	T11376	punct	-,crossed
R7119	T11378	T11376	prt	over,crossed
R712	T1214	T1215	nmod	SWI2,SNF2
R7120	T11379	T11380	punct	(,2A
R7121	T11380	T11327	parataxis	2A,digested
R7122	T11381	T11380	compound	Figure,2A
R7123	T11382	T11380	cc	and,2A
R7124	T11383	T11380	conj	2C,2A
R7125	T11384	T11380	punct	),2A
R7126	T11385	T11327	punct	.,digested
R7127	T11387	T11388	nsubjpass	Sequences,shown
R7128	T11389	T11387	prep	of,Sequences
R7129	T11390	T11389	pobj	primers,of
R713	T1215	T1213	nmod	SNF2,protein
R7130	T11391	T11388	auxpass	are,shown
R7131	T11392	T11388	prep	in,shown
R7132	T11393	T11394	compound	Table,S1
R7133	T11394	T11392	pobj	S1,in
R7134	T11395	T11388	punct	.,shown
R7135	T11569	T11570	compound	Cre,recombination
R7136	T11571	T11570	punct	-,recombination
R7137	T11572	T11570	cc	and,recombination
R7138	T11573	T11570	conj	characterisation,recombination
R7139	T11574	T11570	prep	of,recombination
R714	T1216	T1215	punct	/,SNF2
R7140	T11575	T11576	amod	Atrxnull,cells
R7141	T11576	T11574	pobj	cells,of
R7142	T11577	T11576	compound	ES,cells
R7143	T11578	T11576	cc	and,cells
R7144	T11579	T11580	amod	embryoid,bodies
R7145	T11580	T11576	conj	bodies,cells
R7146	T11581	T11570	punct	.,recombination
R7147	T11583	T11584	compound	ES,cell
R7148	T11584	T11585	compound	cell,clones
R7149	T11585	T11586	nsubjpass	clones,transfected
R715	T1217	T1218	npadvmod	chromatin,remodelling
R7150	T11587	T11585	acl	bearing,clones
R7151	T11588	T11589	det	the,allele
R7152	T11589	T11587	dobj	allele,bearing
R7153	T11590	T11589	compound	Atrx flox,allele
R7154	T11591	T11592	punct	(,cells
R7155	T11592	T11585	parataxis	cells,clones
R7156	T11593	T11594	quantmod	1,107
R7157	T11594	T11592	nummod	107,cells
R7158	T11595	T11594	punct	×,107
R7159	T11596	T11592	punct	),cells
R716	T1218	T1213	amod	remodelling,protein
R7160	T11597	T11586	auxpass	were,transfected
R7161	T11598	T11586	advmod	transiently,transfected
R7162	T11599	T11586	prep	with,transfected
R7163	T11600	T11601	nummod	50,μg
R7164	T11601	T11599	pobj	μg,with
R7165	T11602	T11601	prep	of,μg
R7166	T11603	T11604	amod	uncut,plasmid
R7167	T11604	T11602	pobj	plasmid,of
R7168	T11605	T11606	compound	Cre,expression
R7169	T11606	T11604	compound	expression,plasmid
R717	T1219	T1218	punct	-,remodelling
R7170	T11607	T11604	punct	(,plasmid
R7171	T11608	T11609	compound	pCAGGS,IRESpuro
R7172	T11609	T11604	appos	IRESpuro,plasmid
R7173	T11610	T11609	punct	-,IRESpuro
R7174	T11611	T11609	compound	Cre,IRESpuro
R7175	T11612	T11609	punct	-,IRESpuro
R7176	T11613	T11586	punct	),transfected
R7177	T11614	T11615	punct	[,40
R7178	T11615	T11586	parataxis	40,transfected
R7179	T11616	T11615	punct	],40
R718	T1220	T1209	punct	", ",regulates
R7180	T11617	T11586	punct	.,transfected
R7181	T11619	T11620	prep	Following,plated
R7182	T11621	T11619	pobj	transfection,Following
R7183	T11622	T11620	punct	", ",plated
R7184	T11623	T11620	nsubjpass	cells,plated
R7185	T11624	T11620	auxpass	were,plated
R7186	T11625	T11620	prep	at,plated
R7187	T11626	T11627	det	a,range
R7188	T11627	T11625	pobj	range,at
R7189	T11628	T11627	prep	of,range
R719	T1221	T1209	nsubj	ATRX,regulates
R7190	T11629	T11630	amod	clonal,densities
R7191	T11630	T11628	pobj	densities,of
R7192	T11631	T11620	prep	in,plated
R7193	T11632	T11633	amod	complete,medium
R7194	T11633	T11631	pobj	medium,in
R7195	T11634	T11633	prep	without,medium
R7196	T11635	T11634	pobj	G418,without
R7197	T11636	T11620	punct	", ",plated
R7198	T11637	T11620	cc	and,plated
R7199	T11638	T11639	amod	isolated,subclones
R72	T233	T232	amod	associated,abnormalities
R720	T1222	T1209	advmod	probably,regulates
R7200	T11639	T11640	nsubjpass	subclones,picked
R7201	T11640	T11620	conj	picked,plated
R7202	T11641	T11640	auxpass	were,picked
R7203	T11642	T11640	prep	after,picked
R7204	T11643	T11644	nummod	7,d
R7205	T11644	T11642	pobj	d,after
R7206	T11645	T11620	punct	.,plated
R7207	T11647	T11648	nsubjpass	Subclones,expanded
R7208	T11649	T11648	auxpass	were,expanded
R7209	T11650	T11648	cc	and,expanded
R721	T1223	T1209	dobj	transcription,regulates
R7210	T11651	T11648	conj	analysed,expanded
R7211	T11652	T11651	prep	for,analysed
R7212	T11653	T11654	det	the,presence
R7213	T11654	T11652	pobj	presence,for
R7214	T11655	T11654	prep	of,presence
R7215	T11656	T11657	det	a,locus
R7216	T11657	T11655	pobj	locus,of
R7217	T11658	T11657	amod	recombinant,locus
R7218	T11659	T11660	advmod	initially,by
R7219	T11660	T11651	prep	by,analysed
R722	T1224	T1223	prep	of,transcription
R7220	T11661	T11660	pobj	PCR,by
R7221	T11662	T11660	punct	", ",by
R7222	T11663	T11664	aux	to,detect
R7223	T11664	T11660	advcl	detect,by
R7224	T11665	T11664	dobj	deletion,detect
R7225	T11666	T11665	prep	of,deletion
R7226	T11667	T11668	det	the,cassette
R7227	T11668	T11666	pobj	cassette,of
R7228	T11669	T11668	compound	MC1neopA,cassette
R7229	T11670	T11660	punct	", ",by
R723	T1225	T1226	det	a,set
R7230	T11671	T11660	cc	and,by
R7231	T11672	T11673	advmod	then,by
R7232	T11673	T11660	conj	by,by
R7233	T11674	T11675	compound	Southern,blot
R7234	T11675	T11673	pobj	blot,by
R7235	T11676	T11675	cc	and,blot
R7236	T11677	T11675	conj	hybridisation,blot
R7237	T11678	T11675	prep	with,blot
R7238	T11679	T11680	det	the,probe
R7239	T11680	T11678	pobj	probe,with
R724	T1226	T1224	pobj	set,of
R7240	T11681	T11680	nmod	intron,probe
R7241	T11682	T11681	nummod	17,intron
R7242	T11683	T11680	acl	described,probe
R7243	T11684	T11683	advmod	above,described
R7244	T11685	T11686	punct	(,2A
R7245	T11686	T11651	parataxis	2A,analysed
R7246	T11687	T11686	compound	Figure,2A
R7247	T11688	T11686	cc	and,2A
R7248	T11689	T11686	conj	2C,2A
R7249	T11690	T11686	punct	),2A
R725	T1227	T1226	amod	discrete,set
R7250	T11691	T11648	punct	.,expanded
R7251	T11693	T11694	compound	Northern,blots
R7252	T11694	T11695	nsubjpass	blots,carried
R7253	T11696	T11695	auxpass	were,carried
R7254	T11697	T11695	prt	out,carried
R7255	T11698	T11695	prep	according,carried
R7256	T11699	T11698	prep	to,according
R7257	T11700	T11701	amod	standard,techniques
R7258	T11701	T11699	pobj	techniques,to
R7259	T11702	T11695	advcl	using,carried
R726	T1228	T1226	prep	of,set
R7260	T11703	T11704	nummod	20,μg
R7261	T11704	T11702	dobj	μg,using
R7262	T11705	T11704	prep	of,μg
R7263	T11706	T11707	amod	total,RNA
R7264	T11707	T11705	pobj	RNA,of
R7265	T11708	T11707	acl	isolated,RNA
R7266	T11709	T11708	advcl	using,isolated
R7267	T11710	T11711	compound	TRI,Reagent
R7268	T11711	T11709	dobj	Reagent,using
R7269	T11712	T11713	punct	(,Aldrich
R727	T1229	T1230	compound	target,genes
R7270	T11713	T11711	parataxis	Aldrich,Reagent
R7271	T11714	T11713	compound	Sigma,Aldrich
R7272	T11715	T11713	punct	-,Aldrich
R7273	T11716	T11713	punct	", ",Aldrich
R7274	T11717	T11718	compound	St.,Louis
R7275	T11718	T11713	npadvmod	Louis,Aldrich
R7276	T11719	T11713	punct	", ",Aldrich
R7277	T11720	T11713	npadvmod	Missouri,Aldrich
R7278	T11721	T11713	punct	", ",Aldrich
R7279	T11722	T11723	compound	United,States
R728	T1230	T1228	pobj	genes,of
R7280	T11723	T11713	npadvmod	States,Aldrich
R7281	T11724	T11713	punct	),Aldrich
R7282	T11725	T11695	punct	.,carried
R7283	T11727	T11728	det	The,blot
R7284	T11728	T11729	nsubjpass	blot,hybridised
R7285	T11730	T11729	auxpass	was,hybridised
R7286	T11731	T11729	prep	with,hybridised
R7287	T11732	T11733	det	a,probe
R7288	T11733	T11731	pobj	probe,with
R7289	T11734	T11733	prep	from,probe
R729	T1231	T1196	punct	.,suggests
R7290	T11735	T11734	prep	within,from
R7291	T11736	T11735	pobj	exon,within
R7292	T11737	T11736	nummod	10,exon
R7293	T11738	T11736	prep	of,exon
R7294	T11739	T11740	det	the,gene
R7295	T11740	T11738	pobj	gene,of
R7296	T11741	T11740	compound	Atrx,gene
R7297	T11742	T11733	punct	(,probe
R7298	T11743	T11733	acl	generated,probe
R7299	T11744	T11743	prep	with,generated
R73	T234	T232	prep	in,abnormalities
R730	T1233	T1234	advmod	However,remain
R7300	T11745	T11746	compound	primers,Mxnp4
R7301	T11746	T11744	pobj	Mxnp4,with
R7302	T11747	T11746	cc	and,Mxnp4
R7303	T11748	T11746	conj	Mxnp28,Mxnp4
R7304	T11749	T11750	punct	[,S1
R7305	T11750	T11729	parataxis	S1,hybridised
R7306	T11751	T11750	compound	Table,S1
R7307	T11752	T11750	punct	],S1
R7308	T11753	T11729	punct	),hybridised
R7309	T11754	T11729	punct	.,hybridised
R731	T1235	T1234	punct	", ",remain
R7310	T11756	T11757	mark	After,stripped
R7311	T11757	T11760	advcl	stripped,hybridised
R7312	T11758	T11757	nsubjpass	it,stripped
R7313	T11759	T11757	auxpass	was,stripped
R7314	T11761	T11760	punct	", ",hybridised
R7315	T11762	T11763	det	the,membrane
R7316	T11763	T11760	nsubjpass	membrane,hybridised
R7317	T11764	T11760	auxpass	was,hybridised
R7318	T11765	T11760	prep	with,hybridised
R7319	T11766	T11767	det	a,probe
R732	T1236	T1237	mark	although,are
R7320	T11767	T11765	pobj	probe,with
R7321	T11768	T11769	compound	β,actin
R7322	T11769	T11767	compound	actin,probe
R7323	T11770	T11769	punct	-,actin
R7324	T11771	T11767	compound	cDNA,probe
R7325	T11772	T11773	punct	(,Clontech
R7326	T11773	T11767	parataxis	Clontech,probe
R7327	T11774	T11773	punct	", ",Clontech
R7328	T11775	T11776	compound	Palo,Alto
R7329	T11776	T11773	npadvmod	Alto,Clontech
R733	T1237	T1234	advcl	are,remain
R7330	T11777	T11773	punct	", ",Clontech
R7331	T11778	T11773	npadvmod	California,Clontech
R7332	T11779	T11773	punct	", ",Clontech
R7333	T11780	T11781	compound	United,States
R7334	T11781	T11773	npadvmod	States,Clontech
R7335	T11782	T11773	punct	),Clontech
R7336	T11783	T11760	punct	.,hybridised
R7337	T11785	T11786	compound	Protein,extraction
R7338	T11786	T11787	nsubjpass	extraction,performed
R7339	T11788	T11786	cc	and,extraction
R734	T1238	T1237	expl	there,are
R7340	T11789	T11786	conj	detection,extraction
R7341	T11790	T11789	prep	of,detection
R7342	T11791	T11790	pobj	Atrx,of
R7343	T11792	T11789	prep	by,detection
R7344	T11793	T11794	compound	Western,blotting
R7345	T11794	T11792	pobj	blotting,by
R7346	T11795	T11787	auxpass	was,performed
R7347	T11796	T11797	mark	as,described
R7348	T11797	T11787	advcl	described,performed
R7349	T11798	T11797	advmod	previously,described
R735	T1239	T1237	advmod	clearly,are
R7350	T11799	T11800	punct	[,4
R7351	T11800	T11797	parataxis	4,described
R7352	T11801	T11800	punct	],4
R7353	T11802	T11787	punct	", ",performed
R7354	T11803	T11787	advcl	using,performed
R7355	T11804	T11805	det	the,antibody
R7356	T11805	T11803	dobj	antibody,using
R7357	T11806	T11805	nmod	mouse,antibody
R7358	T11807	T11805	amod	monoclonal,antibody
R7359	T11808	T11805	amod	anti-ATRX,antibody
R736	T1240	T1237	attr	others,are
R7360	T11809	T11805	appos	23C,antibody
R7361	T11810	T11811	punct	[,15
R7362	T11811	T11787	parataxis	15,performed
R7363	T11812	T11811	punct	],15
R7364	T11813	T11787	punct	.,performed
R7365	T11815	T11816	nsubjpass	Analyses,described
R7366	T11817	T11815	prep	of,Analyses
R7367	T11818	T11819	compound	cell,cycle
R7368	T11819	T11817	pobj	cycle,of
R7369	T11820	T11819	cc	and,cycle
R737	T1241	T1242	aux	to,found
R7370	T11821	T11819	conj	apoptosis,cycle
R7371	T11822	T11816	auxpass	are,described
R7372	T11823	T11816	prep	in,described
R7373	T11824	T11825	compound	Protocol,S1
R7374	T11825	T11823	pobj	S1,in
R7375	T11826	T11816	punct	.,described
R7376	T11828	T11829	nsubjpass	Methylation,analysed
R7377	T11830	T11828	prep	of,Methylation
R7378	T11831	T11830	pobj	rDNA,of
R7379	T11832	T11829	auxpass	was,analysed
R738	T1242	T1240	advcl	found,others
R7380	T11833	T11829	prep	in,analysed
R7381	T11834	T11833	pobj	DNA,in
R7382	T11835	T11834	prep	from,DNA
R7383	T11836	T11837	compound	ES,cell
R7384	T11837	T11838	compound	cell,clones
R7385	T11838	T11835	pobj	clones,from
R7386	T11839	T11835	cc	or,from
R7387	T11840	T11835	conj	from,from
R7388	T11841	T11842	amod	embryoid,bodies
R7389	T11842	T11840	pobj	bodies,from
R739	T1243	T1242	auxpass	be,found
R7390	T11843	T11842	acl	recovered,bodies
R7391	T11844	T11843	prep	after,recovered
R7392	T11845	T11846	nummod	7,d
R7393	T11846	T11844	pobj	d,after
R7394	T11847	T11846	prep	of,d
R7395	T11848	T11849	advmod	in,vitro
R7396	T11849	T11850	amod	vitro,differentiation
R7397	T11850	T11847	pobj	differentiation,of
R7398	T11851	T11852	mark	as,described
R7399	T11852	T11829	advcl	described,analysed
R74	T235	T236	det	the,syndrome
R740	T1244	T1234	punct	", ",remain
R7400	T11853	T11852	advmod	previously,described
R7401	T11854	T11855	punct	[,41
R7402	T11855	T11829	parataxis	41,analysed
R7403	T11856	T11855	punct	],41
R7404	T11857	T11829	punct	.,analysed
R7405	T11859	T11860	amod	Genomic,DNA
R7406	T11860	T11861	nsubjpass	DNA,digested
R7407	T11862	T11861	auxpass	was,digested
R7408	T11863	T11861	prep	with,digested
R7409	T11864	T11865	npadvmod	methylation,sensitive
R741	T1245	T1234	prep	at,remain
R7410	T11865	T11867	amod	sensitive,enzymes
R7411	T11866	T11865	punct	-,sensitive
R7412	T11867	T11863	pobj	enzymes,with
R7413	T11868	T11867	compound	restriction,enzymes
R7414	T11869	T11870	mark	as,described
R7415	T11870	T11861	advcl	described,digested
R7416	T11871	T11861	cc	and,digested
R7417	T11872	T11861	conj	analysed,digested
R7418	T11873	T11872	prep	by,analysed
R7419	T11874	T11875	compound	Southern,blotting
R742	T1246	T1245	amod	present,at
R7420	T11875	T11873	pobj	blotting,by
R7421	T11876	T11861	punct	.,digested
R7422	T11878	T11879	det	The,probes
R7423	T11879	T11883	nsubjpass	probes,described
R7424	T11880	T11879	nmod	RIB3,probes
R7425	T11881	T11880	cc	and,RIB3
R7426	T11882	T11880	conj	RIB4,RIB3
R7427	T11884	T11879	punct	(,probes
R7428	T11885	T11886	dep	which,amplified
R7429	T11886	T11879	relcl	amplified,probes
R743	T1247	T1248	det	the,genes
R7430	T11887	T11886	auxpass	were,amplified
R7431	T11888	T11886	prep	from,amplified
R7432	T11889	T11890	amod	human,DNA
R7433	T11890	T11888	pobj	DNA,from
R7434	T11891	T11886	punct	", ",amplified
R7435	T11892	T11886	cc	but,amplified
R7436	T11893	T11886	conj	cross-react,amplified
R7437	T11894	T11893	prep	with,cross-react
R7438	T11895	T11896	det	the,repeat
R7439	T11896	T11894	pobj	repeat,with
R744	T1248	T1234	nsubj	genes,remain
R7440	T11897	T11898	compound	mouse,rDNA
R7441	T11898	T11896	compound	rDNA,repeat
R7442	T11899	T11879	punct	),probes
R7443	T11900	T11883	aux	have,described
R7444	T11901	T11883	auxpass	been,described
R7445	T11902	T11883	advmod	previously,described
R7446	T11903	T11904	punct	[,11
R7447	T11904	T11883	parataxis	11,described
R7448	T11905	T11904	punct	],11
R7449	T11906	T11883	punct	.,described
R745	T1249	T1250	compound	α,globin
R7450	T11908	T11909	compound	Oligonucleotide,probes
R7451	T11909	T11910	nsubjpass	probes,described
R7452	T11911	T11912	aux	to,detect
R7453	T11912	T11909	advcl	detect,probes
R7454	T11913	T11914	nmod	Line,repeats
R7455	T11914	T11912	dobj	repeats,detect
R7456	T11915	T11913	nummod	1,Line
R7457	T11916	T11913	cc	and,Line
R7458	T11917	T11918	compound	Sine,B1
R7459	T11918	T11913	conj	B1,Line
R746	T1250	T1248	compound	globin,genes
R7460	T11919	T11910	aux	have,described
R7461	T11920	T11910	auxpass	been,described
R7462	T11921	T11910	advmod	previously,described
R7463	T11922	T11923	punct	[,42
R7464	T11923	T11910	parataxis	42,described
R7465	T11924	T11923	punct	],42
R7466	T11925	T11910	punct	.,described
R7467	T11927	T11928	det	The,probe
R7468	T11928	T11931	nsubj	probe,was
R7469	T11929	T11930	amod	minor,satellite
R747	T1251	T1250	punct	-,globin
R7470	T11930	T11928	compound	satellite,probe
R7471	T11932	T11933	det	a,oligonucleotide
R7472	T11933	T11931	attr	oligonucleotide,was
R7473	T11934	T11935	nummod	27,mer
R7474	T11935	T11933	compound	mer,oligonucleotide
R7475	T11936	T11935	punct	-,mer
R7476	T11937	T11933	punct	(,oligonucleotide
R7477	T11938	T11933	appos	mCENT2,oligonucleotide
R7478	T11939	T11931	punct	),was
R7479	T11940	T11931	punct	.,was
R748	T1252	T1253	det	the,targets
R7480	T11942	T11943	det	The,probe
R7481	T11943	T11946	nsubj	probe,was
R7482	T11944	T11945	amod	major,satellite
R7483	T11945	T11943	compound	satellite,probe
R7484	T11947	T11948	det	a,oligonucleotide
R7485	T11948	T11946	attr	oligonucleotide,was
R7486	T11949	T11950	nummod	27,mer
R7487	T11950	T11948	compound	mer,oligonucleotide
R7488	T11951	T11950	punct	-,mer
R7489	T11952	T11948	punct	(,oligonucleotide
R749	T1253	T1234	oprd	targets,remain
R7490	T11953	T11948	appos	DG27,oligonucleotide
R7491	T11954	T11946	punct	),was
R7492	T11955	T11946	punct	.,was
R7493	T11957	T11958	det	The,probe
R7494	T11958	T11960	nsubj	probe,was
R7495	T11959	T11958	compound	IAP,probe
R7496	T11961	T11962	det	an,product
R7497	T11962	T11960	attr	product,was
R7498	T11963	T11964	punct	~,400
R7499	T11964	T11965	nummod	400,bp
R75	T236	T234	pobj	syndrome,in
R750	T1254	T1253	advmod	only,targets
R7500	T11965	T11966	compound	bp,PCR
R7501	T11966	T11962	compound	PCR,product
R7502	T11967	T11962	punct	(,product
R7503	T11968	T11969	compound	primers,14A
R7504	T11969	T11962	appos	14A,product
R7505	T11970	T11969	cc	and,14A
R7506	T11971	T11969	conj	13K,14A
R7507	T11972	T11962	punct	),product
R7508	T11973	T11962	acl	amplified,product
R7509	T11974	T11973	prep	from,amplified
R751	T1255	T1253	amod	confirmed,targets
R7510	T11975	T11976	det	an,IAP
R7511	T11976	T11974	pobj	IAP,from
R7512	T11977	T11976	acl	inserted,IAP
R7513	T11978	T11977	prep	into,inserted
R7514	T11979	T11980	det	the,gene
R7515	T11980	T11978	pobj	gene,into
R7516	T11981	T11982	compound	mouse,agouti
R7517	T11982	T11980	compound	agouti,gene
R7518	T11983	T11984	punct	[,43
R7519	T11984	T11960	parataxis	43,was
R752	T1256	T1253	prep	for,targets
R7520	T11985	T11984	punct	],43
R7521	T11986	T11960	cc	and,was
R7522	T11987	T11960	conj	was,was
R7523	T11988	T11989	det	a,gift
R7524	T11989	T11987	attr	gift,was
R7525	T11990	T11989	prep	from,gift
R7526	T11991	T11992	compound	Peter,Warnecke
R7527	T11992	T11990	pobj	Warnecke,from
R7528	T11993	T11992	cc	and,Warnecke
R7529	T11994	T11995	compound	Tim,Bestor
R753	T1257	T1258	amod	transcriptional,regulation
R7530	T11995	T11992	conj	Bestor,Warnecke
R7531	T11996	T11960	punct	.,was
R7532	T11998	T11999	det	The,product
R7533	T11999	T12001	nsubj	product,included
R7534	T12000	T11999	compound	PCR,product
R7535	T12002	T12003	det	the,LTR
R7536	T12003	T12001	dobj	LTR,included
R7537	T12004	T12003	amod	entire,LTR
R7538	T12005	T12003	nummod	5,LTR
R7539	T12006	T12005	punct	′,5
R754	T1258	T1256	pobj	regulation,for
R7540	T12007	T12003	prep	of,LTR
R7541	T12008	T12009	det	the,IAP
R7542	T12009	T12007	pobj	IAP,of
R7543	T12010	T12001	punct	.,included
R7544	T12012	T12013	det	All,sequences
R7545	T12013	T12015	nsubjpass	sequences,shown
R7546	T12014	T12013	compound	oligonucleotide,sequences
R7547	T12016	T12015	auxpass	are,shown
R7548	T12017	T12015	prep	in,shown
R7549	T12018	T12019	compound	Table,S1
R755	T1259	T1258	prep	by,regulation
R7550	T12019	T12017	pobj	S1,in
R7551	T12020	T12015	punct	.,shown
R7552	T12081	T12080	prep	of,Generation
R7553	T12082	T12081	pobj	chimeras,of
R7554	T12083	T12082	punct	", ",chimeras
R7555	T12084	T12085	amod	floxed,mice
R7556	T12085	T12082	conj	mice,chimeras
R7557	T12086	T12085	punct	", ",mice
R7558	T12087	T12085	cc	and,mice
R7559	T12088	T12089	compound	mutant,mice
R756	T1260	T1259	pobj	ATRX,by
R7560	T12089	T12085	conj	mice,mice
R7561	T12090	T12080	punct	.,Generation
R7562	T12092	T12093	amod	Targeted,clones
R7563	T12093	T12097	nsubjpass	clones,injected
R7564	T12094	T12095	compound	Atrx flox,cell
R7565	T12095	T12093	compound	cell,clones
R7566	T12096	T12095	compound	ES,cell
R7567	T12098	T12097	auxpass	were,injected
R7568	T12099	T12097	prep	into,injected
R7569	T12100	T12101	nmod	C57BL,blastocysts
R757	T1261	T1234	punct	.,remain
R7570	T12101	T12099	pobj	blastocysts,into
R7571	T12102	T12100	punct	/,C57BL
R7572	T12103	T12100	nummod	6,C57BL
R7573	T12104	T12097	cc	and,injected
R7574	T12105	T12097	conj	transferred,injected
R7575	T12106	T12105	prep	into,transferred
R7576	T12107	T12108	nummod	2.5,dpc
R7577	T12108	T12109	nmod	dpc,recipients
R7578	T12109	T12106	pobj	recipients,into
R7579	T12110	T12109	amod	pseudopregnant,recipients
R758	T1263	T1264	nsubjpass	Little,known
R7580	T12111	T12097	prep	by,injected
R7581	T12112	T12113	amod	standard,techniques
R7582	T12113	T12111	pobj	techniques,by
R7583	T12114	T12097	punct	.,injected
R7584	T12116	T12117	amod	Resulting,chimeras
R7585	T12117	T12118	nsubjpass	chimeras,mated
R7586	T12119	T12118	auxpass	were,mated
R7587	T12120	T12118	prep	with,mated
R7588	T12121	T12120	pobj	C57BL,with
R7589	T12122	T12121	punct	/,C57BL
R759	T1265	T1264	auxpass	is,known
R7590	T12123	T12121	nummod	6,C57BL
R7591	T12124	T12125	aux	to,establish
R7592	T12125	T12118	advcl	establish,mated
R7593	T12126	T12127	compound	germline,transmission
R7594	T12127	T12125	dobj	transmission,establish
R7595	T12128	T12118	punct	.,mated
R7596	T12130	T12131	nsubjpass	Offspring,identified
R7597	T12132	T12130	prep	with,Offspring
R7598	T12133	T12134	det	the,allele
R7599	T12134	T12132	pobj	allele,with
R76	T237	T238	nmod	ATR,X
R760	T1266	T1264	advmod	currently,known
R7600	T12135	T12134	compound	Atrx flox,allele
R7601	T12136	T12131	auxpass	were,identified
R7602	T12137	T12131	prep	by,identified
R7603	T12138	T12139	compound	Southern,blotting
R7604	T12139	T12137	pobj	blotting,by
R7605	T12140	T12139	prep	of,blotting
R7606	T12141	T12142	npadvmod	SacI,digested
R7607	T12142	T12144	amod	digested,DNA
R7608	T12143	T12142	punct	-,digested
R7609	T12144	T12140	pobj	DNA,of
R761	T1267	T1264	prep	about,known
R7610	T12145	T12144	compound	tail,DNA
R7611	T12146	T12131	advcl	using,identified
R7612	T12147	T12148	det	the,probe
R7613	T12148	T12146	dobj	probe,using
R7614	T12149	T12148	nmod	intron,probe
R7615	T12150	T12149	nummod	17,intron
R7616	T12151	T12152	mark	as,shown
R7617	T12152	T12146	advcl	shown,using
R7618	T12153	T12152	prep	in,shown
R7619	T12154	T12155	compound	Figure,2A
R762	T1268	T1269	det	the,role
R7620	T12155	T12153	pobj	2A,in
R7621	T12156	T12155	cc	and,2A
R7622	T12157	T12155	conj	2C,2A
R7623	T12158	T12131	punct	.,identified
R7624	T12160	T12161	prep	For,crossed
R7625	T12162	T12163	compound	Cre,recombination
R7626	T12163	T12160	pobj	recombination,For
R7627	T12164	T12163	punct	-,recombination
R7628	T12165	T12161	punct	", ",crossed
R7629	T12166	T12167	compound	Atrx flox,mice
R763	T1269	T1267	pobj	role,about
R7630	T12167	T12161	nsubjpass	mice,crossed
R7631	T12168	T12161	auxpass	were,crossed
R7632	T12169	T12161	prep	with,crossed
R7633	T12170	T12171	compound	GATA1,Cre
R7634	T12171	T12173	npadvmod	Cre,transgenic
R7635	T12172	T12171	punct	-,Cre
R7636	T12173	T12174	amod	transgenic,mice
R7637	T12174	T12169	pobj	mice,with
R7638	T12175	T12176	mark	as,described
R7639	T12176	T12161	advcl	described,crossed
R764	T1270	T1269	amod	precise,role
R7640	T12177	T12176	prep	in,described
R7641	T12178	T12179	det	the,text
R7642	T12179	T12177	pobj	text,in
R7643	T12180	T12161	punct	.,crossed
R7644	T12182	T12183	amod	Recombinant,alleles
R7645	T12183	T12184	nsubjpass	alleles,detected
R7646	T12185	T12184	auxpass	were,detected
R7647	T12186	T12184	prep	by,detected
R7648	T12187	T12188	compound	Southern,blotting
R7649	T12188	T12186	pobj	blotting,by
R765	T1271	T1269	prep	of,role
R7650	T12189	T12190	mark	as,described
R7651	T12190	T12188	advcl	described,blotting
R7652	T12191	T12190	advmod	above,described
R7653	T12192	T12186	cc	or,by
R7654	T12193	T12186	conj	by,by
R7655	T12194	T12193	pobj	PCR,by
R7656	T12195	T12196	mark	as,described
R7657	T12196	T12184	advcl	described,detected
R7658	T12197	T12196	prep	in,described
R7659	T12198	T12199	compound	Protocol,S1
R766	T1272	T1273	det	the,protein
R7660	T12199	T12197	pobj	S1,in
R7661	T12200	T12184	punct	.,detected
R7662	T12293	T12292	punct	", ",Immunohistochemistry
R7663	T12294	T12295	nmod	in,situ
R7664	T12295	T12296	nmod	situ,hybridisation
R7665	T12296	T12292	conj	hybridisation,Immunohistochemistry
R7666	T12297	T12296	punct	", ",hybridisation
R7667	T12298	T12296	cc	and,hybridisation
R7668	T12299	T12300	compound	TUNEL,assay
R7669	T12300	T12296	conj	assay,hybridisation
R767	T1273	T1271	pobj	protein,of
R7670	T12301	T12300	punct	.,assay
R7671	T12303	T12304	nummod	7.5,dpc
R7672	T12304	T12305	nmod	dpc,swellings
R7673	T12305	T12307	nsubjpass	swellings,dissected
R7674	T12306	T12305	amod	decidual,swellings
R7675	T12308	T12307	auxpass	were,dissected
R7676	T12309	T12307	advmod	away,dissected
R7677	T12310	T12309	prep	from,away
R7678	T12311	T12312	amod	maternal,tissue
R7679	T12312	T12310	pobj	tissue,from
R768	T1274	T1273	compound	ATRX,protein
R7680	T12313	T12307	cc	and,dissected
R7681	T12314	T12307	conj	fixed,dissected
R7682	T12315	T12314	prep	in,fixed
R7683	T12316	T12317	nummod	4,%
R7684	T12317	T12318	compound	%,paraformaldehyde
R7685	T12318	T12315	pobj	paraformaldehyde,in
R7686	T12319	T12318	punct	/,paraformaldehyde
R7687	T12320	T12318	appos	PBS,paraformaldehyde
R7688	T12321	T12314	advmod	overnight,fixed
R7689	T12322	T12314	prep	at,fixed
R769	T1275	T1269	prep	during,role
R7690	T12323	T12324	nummod	4,°C
R7691	T12324	T12322	pobj	°C,at
R7692	T12325	T12307	punct	.,dissected
R7693	T12327	T12328	mark	After,washed
R7694	T12328	T12331	advcl	washed,dehydrated
R7695	T12329	T12328	nsubjpass	embryos,washed
R7696	T12330	T12328	auxpass	were,washed
R7697	T12332	T12328	advmod	thoroughly,washed
R7698	T12333	T12328	prep	in,washed
R7699	T12334	T12333	pobj	PBS,in
R77	T238	T236	nmod	X,syndrome
R770	T1276	T1277	amod	mammalian,development
R7700	T12335	T12331	punct	", ",dehydrated
R7701	T12336	T12331	nsubjpass	they,dehydrated
R7702	T12337	T12331	auxpass	were,dehydrated
R7703	T12338	T12331	prep	through,dehydrated
R7704	T12339	T12340	det	an,series
R7705	T12340	T12338	pobj	series,through
R7706	T12341	T12340	compound	ethanol,series
R7707	T12342	T12340	cc	and,series
R7708	T12343	T12340	conj	xylene,series
R7709	T12344	T12331	punct	", ",dehydrated
R771	T1277	T1275	pobj	development,during
R7710	T12345	T12331	conj	embedded,dehydrated
R7711	T12346	T12345	prep	in,embedded
R7712	T12347	T12346	pobj	paraffin,in
R7713	T12348	T12345	punct	", ",embedded
R7714	T12349	T12345	cc	and,embedded
R7715	T12350	T12345	conj	sectioned,embedded
R7716	T12351	T12350	prep	at,sectioned
R7717	T12352	T12353	nummod	5,μm
R7718	T12353	T12351	pobj	μm,at
R7719	T12354	T12331	punct	.,dehydrated
R772	T1278	T1264	punct	.,known
R7720	T12356	T12357	nsubjpass	Sections,processed
R7721	T12358	T12357	auxpass	were,processed
R7722	T12359	T12357	prep	for,processed
R7723	T12360	T12359	pobj	immunohistochemistry,for
R7724	T12361	T12357	advcl	using,processed
R7725	T12362	T12363	det	the,System
R7726	T12363	T12361	dobj	System,using
R7727	T12364	T12363	compound	ABC,System
R7728	T12365	T12363	compound	Staining,System
R7729	T12366	T12367	punct	(,Biotechnology
R773	T1280	T1281	aux	To,investigate
R7730	T12367	T12363	parataxis	Biotechnology,System
R7731	T12368	T12369	compound	Santa,Cruz
R7732	T12369	T12367	compound	Cruz,Biotechnology
R7733	T12370	T12367	punct	", ",Biotechnology
R7734	T12371	T12372	compound	Santa,Cruz
R7735	T12372	T12367	npadvmod	Cruz,Biotechnology
R7736	T12373	T12367	punct	", ",Biotechnology
R7737	T12374	T12367	npadvmod	California,Biotechnology
R7738	T12375	T12367	punct	", ",Biotechnology
R7739	T12376	T12377	compound	United,States
R774	T1281	T1282	advcl	investigate,generated
R7740	T12377	T12367	npadvmod	States,Biotechnology
R7741	T12378	T12367	punct	),Biotechnology
R7742	T12379	T12361	prep	according,using
R7743	T12380	T12379	prep	to,according
R7744	T12381	T12382	det	the,manufacturer
R7745	T12382	T12383	poss	manufacturer,instructions
R7746	T12383	T12380	pobj	instructions,to
R7747	T12384	T12382	case	's,manufacturer
R7748	T12385	T12357	punct	.,processed
R7749	T12387	T12388	nsubjpass	Sections,stained
R775	T1283	T1284	det	the,role
R7750	T12389	T12388	auxpass	were,stained
R7751	T12390	T12388	prep	with,stained
R7752	T12391	T12392	nmod	rabbit,antibodies
R7753	T12392	T12390	pobj	antibodies,with
R7754	T12393	T12392	amod	polyclonal,antibodies
R7755	T12394	T12392	prep	against,antibodies
R7756	T12395	T12394	pobj	ATRX,against
R7757	T12396	T12397	punct	(,H
R7758	T12397	T12395	parataxis	H,ATRX
R7759	T12398	T12397	punct	-,H
R776	T1284	T1281	dobj	role,investigate
R7760	T12399	T12397	nummod	300,H
R7761	T12400	T12397	punct	", ",H
R7762	T12401	T12402	compound	Santa,Cruz
R7763	T12402	T12403	compound	Cruz,Biotechnology
R7764	T12403	T12397	npadvmod	Biotechnology,H
R7765	T12404	T12397	punct	),H
R7766	T12405	T12395	punct	", ",ATRX
R7767	T12406	T12407	amod	Placental,I
R7768	T12407	T12395	conj	I,ATRX
R7769	T12408	T12407	compound	lactogen,I
R777	T1285	T1284	prep	of,role
R7770	T12409	T12407	punct	-,I
R7771	T12410	T12411	punct	(,AB1288
R7772	T12411	T12407	parataxis	AB1288,I
R7773	T12412	T12411	punct	", ",AB1288
R7774	T12413	T12414	compound	Chemicon,International
R7775	T12414	T12411	npadvmod	International,AB1288
R7776	T12415	T12411	punct	", ",AB1288
R7777	T12416	T12411	npadvmod	Temecula,AB1288
R7778	T12417	T12411	punct	", ",AB1288
R7779	T12418	T12411	npadvmod	California,AB1288
R778	T1286	T1287	det	this,protein
R7780	T12419	T12411	punct	", ",AB1288
R7781	T12420	T12421	compound	United,States
R7782	T12421	T12411	npadvmod	States,AB1288
R7783	T12422	T12411	punct	),AB1288
R7784	T12423	T12407	cc	and,I
R7785	T12424	T12425	nmod	phospho,histone
R7786	T12425	T12430	compound	histone,H3
R7787	T12426	T12427	punct	(,Ser10
R7788	T12427	T12425	parataxis	Ser10,histone
R7789	T12428	T12427	punct	),Ser10
R779	T1287	T1285	pobj	protein,of
R7790	T12429	T12425	punct	-,histone
R7791	T12430	T12407	conj	H3,I
R7792	T12431	T12432	punct	(,570
R7793	T12432	T12430	parataxis	570,H3
R7794	T12433	T12432	nummod	06,570
R7795	T12434	T12432	punct	–,570
R7796	T12435	T12432	punct	", ",570
R7797	T12436	T12437	compound	Upstate,Biotechnology
R7798	T12437	T12432	npadvmod	Biotechnology,570
R7799	T12438	T12432	punct	", ",570
R78	T239	T238	punct	-,X
R780	T1288	T1284	prep	during,role
R7800	T12439	T12432	npadvmod	Waltham,570
R7801	T12440	T12432	punct	", ",570
R7802	T12441	T12432	npadvmod	Massachusetts,570
R7803	T12442	T12432	punct	", ",570
R7804	T12443	T12444	compound	United,States
R7805	T12444	T12432	npadvmod	States,570
R7806	T12445	T12432	punct	),570
R7807	T12446	T12388	punct	.,stained
R7808	T12448	T12449	advmod	Where,appropriate
R7809	T12449	T12450	advcl	appropriate,stained
R781	T1289	T1290	compound	mouse,development
R7810	T12451	T12450	punct	", ",stained
R7811	T12452	T12453	amod	adjacent,sections
R7812	T12453	T12450	nsubjpass	sections,stained
R7813	T12454	T12450	auxpass	were,stained
R7814	T12455	T12450	prep	with,stained
R7815	T12456	T12455	pobj	haematoxylin,with
R7816	T12457	T12450	punct	.,stained
R7817	T12459	T12460	prep	In,analysed
R7818	T12461	T12462	det	some,cases
R7819	T12462	T12459	pobj	cases,In
R782	T1290	T1288	pobj	development,during
R7820	T12463	T12460	punct	", ",analysed
R7821	T12464	T12465	amod	adjacent,sections
R7822	T12465	T12460	nsubjpass	sections,analysed
R7823	T12466	T12460	auxpass	were,analysed
R7824	T12467	T12460	advmod	also,analysed
R7825	T12468	T12469	aux	to,detect
R7826	T12469	T12460	advcl	detect,analysed
R7827	T12470	T12471	amod	apoptotic,cells
R7828	T12471	T12469	dobj	cells,detect
R7829	T12472	T12469	prep	by,detect
R783	T1291	T1282	punct	", ",generated
R7830	T12473	T12472	pobj	TUNEL,by
R7831	T12474	T12469	advcl	using,detect
R7832	T12475	T12476	det	the,kit
R7833	T12476	T12474	dobj	kit,using
R7834	T12477	T12478	advmod	in,situ
R7835	T12478	T12476	amod	situ,kit
R7836	T12479	T12480	compound	cell,death
R7837	T12480	T12481	compound	death,detection
R7838	T12481	T12476	compound	detection,kit
R7839	T12482	T12483	punct	(,Roche
R784	T1292	T1282	nsubj	we,generated
R7840	T12483	T12474	parataxis	Roche,using
R7841	T12484	T12483	punct	", ",Roche
R7842	T12485	T12483	npadvmod	Basel,Roche
R7843	T12486	T12483	punct	", ",Roche
R7844	T12487	T12483	npadvmod	Switzerland,Roche
R7845	T12488	T12483	punct	),Roche
R7846	T12489	T12460	punct	.,analysed
R7847	T12491	T12492	prep	After,mounted
R7848	T12493	T12491	pobj	labelling,After
R7849	T12494	T12492	punct	", ",mounted
R785	T1293	T1294	det	a,allele
R7850	T12495	T12496	det	these,slides
R7851	T12496	T12492	nsubjpass	slides,mounted
R7852	T12497	T12492	auxpass	were,mounted
R7853	T12498	T12492	prep	in,mounted
R7854	T12499	T12498	pobj	Vectashield,in
R7855	T12500	T12499	acl	containing,Vectashield
R7856	T12501	T12500	dobj	DAPI,containing
R7857	T12502	T12503	punct	(,Laboratories
R7858	T12503	T12499	parataxis	Laboratories,Vectashield
R7859	T12504	T12503	compound	Vector,Laboratories
R786	T1294	T1282	dobj	allele,generated
R7860	T12505	T12503	punct	", ",Laboratories
R7861	T12506	T12503	npadvmod	Burlingame,Laboratories
R7862	T12507	T12503	punct	", ",Laboratories
R7863	T12508	T12503	npadvmod	California,Laboratories
R7864	T12509	T12503	punct	", ",Laboratories
R7865	T12510	T12511	compound	United,States
R7866	T12511	T12503	npadvmod	States,Laboratories
R7867	T12512	T12503	punct	),Laboratories
R7868	T12513	T12492	cc	and,mounted
R7869	T12514	T12492	conj	visualised,mounted
R787	T1295	T1296	advmod	conditionally,deleted
R7870	T12515	T12514	prep	by,visualised
R7871	T12516	T12517	compound	fluorescence,microscopy
R7872	T12517	T12515	pobj	microscopy,by
R7873	T12518	T12492	punct	.,mounted
R7874	T12520	T12521	amod	Whole,mount
R7875	T12521	T12523	nmod	mount,hybridisations
R7876	T12522	T12521	punct	-,mount
R7877	T12523	T12526	nsubjpass	hybridisations,performed
R7878	T12524	T12525	advmod	in,situ
R7879	T12525	T12523	amod	situ,hybridisations
R788	T1296	T1294	amod	deleted,allele
R7880	T12527	T12526	auxpass	were,performed
R7881	T12528	T12526	prep	on,performed
R7882	T12529	T12530	nummod	8.5,dpc
R7883	T12530	T12531	compound	dpc,embryos
R7884	T12531	T12528	pobj	embryos,on
R7885	T12532	T12531	punct	(,embryos
R7886	T12533	T12531	acl	dissected,embryos
R7887	T12534	T12533	advmod	away,dissected
R7888	T12535	T12534	prep	from,away
R7889	T12536	T12537	amod	maternal,tissues
R789	T1297	T1294	prep	of,allele
R7890	T12537	T12535	pobj	tissues,from
R7891	T12538	T12536	cc	and,maternal
R7892	T12539	T12536	conj	extraembryonic,maternal
R7893	T12540	T12533	punct	),dissected
R7894	T12541	T12533	advcl	using,dissected
R7895	T12542	T12543	det	a,riboprobe
R7896	T12543	T12541	dobj	riboprobe,using
R7897	T12544	T12543	nmod	brachyury,riboprobe
R7898	T12545	T12544	punct	(,brachyury
R7899	T12546	T12544	appos	T,brachyury
R79	T240	T238	punct	(,X
R790	T1298	T1299	det	the,gene
R7900	T12547	T12543	punct	),riboprobe
R7901	T12548	T12549	punct	[,16
R7902	T12549	T12541	parataxis	16,using
R7903	T12550	T12549	punct	],16
R7904	T12551	T12528	cc	and,on
R7905	T12552	T12528	conj	on,on
R7906	T12553	T12554	amod	bisected,sites
R7907	T12554	T12552	pobj	sites,on
R7908	T12555	T12554	amod	decidual,sites
R7909	T12556	T12554	compound	implantation,sites
R791	T1299	T1297	pobj	gene,of
R7910	T12557	T12558	prep	from,removed
R7911	T12558	T12554	relcl	removed,sites
R7912	T12559	T12557	pobj	which,from
R7913	T12560	T12558	nsubjpass	embryos,removed
R7914	T12561	T12562	punct	(,dpc
R7915	T12562	T12560	parataxis	dpc,embryos
R7916	T12563	T12562	nummod	8.5,dpc
R7917	T12564	T12562	punct	),dpc
R7918	T12565	T12558	aux	had,removed
R7919	T12566	T12558	auxpass	been,removed
R792	T1300	T1299	compound	Atrx,gene
R7920	T12567	T12558	advcl	using,removed
R7921	T12568	T12569	det	a,riboprobe
R7922	T12569	T12567	dobj	riboprobe,using
R7923	T12570	T12571	amod	placental,lactogen
R7924	T12571	T12569	nmod	lactogen,riboprobe
R7925	T12572	T12571	punct	-,lactogen
R7926	T12573	T12571	nummod	1,lactogen
R7927	T12574	T12571	punct	(,lactogen
R7928	T12575	T12571	appos	Pl,lactogen
R7929	T12576	T12575	punct	-,Pl
R793	T1301	T1282	prep	in,generated
R7930	T12577	T12575	nummod	1,Pl
R7931	T12578	T12569	punct	),riboprobe
R7932	T12579	T12580	punct	(,see
R7933	T12580	T12558	parataxis	see,removed
R7934	T12581	T12582	compound	Protocol,S1
R7935	T12582	T12580	dobj	S1,see
R7936	T12583	T12580	prep	for,see
R7937	T12584	T12583	pobj	details,for
R7938	T12585	T12580	punct	),see
R7939	T12586	T12526	punct	.,performed
R794	T1302	T1303	nmod	mouse,cells
R7940	T12623	T12624	compound	Blastocyst,outgrowth
R7941	T12624	T12625	compound	outgrowth,cultures
R7942	T12626	T12625	punct	.,cultures
R7943	T12628	T12629	amod	Superovulated,mice
R7944	T12629	T12632	nsubjpass	mice,mated
R7945	T12630	T12629	amod	heterozygous,mice
R7946	T12631	T12629	amod	female,mice
R7947	T12633	T12634	punct	(,flox
R7948	T12634	T12629	parataxis	flox,mice
R7949	T12635	T12634	compound	Atrx WT,flox
R795	T1303	T1301	pobj	cells,in
R7950	T12636	T12634	punct	/,flox
R7951	T12637	T12634	punct	),flox
R7952	T12638	T12632	auxpass	were,mated
R7953	T12639	T12632	prep	to,mated
R7954	T12640	T12641	amod	homozygous,males
R7955	T12641	T12639	pobj	males,to
R7956	T12642	T12643	nmod	GATA1,cre
R7957	T12643	T12641	nmod	cre,males
R7958	T12644	T12643	punct	-,cre
R7959	T12645	T12643	punct	+,cre
R796	T1304	T1305	amod	embryonic,stem
R7960	T12646	T12643	punct	/,cre
R7961	T12647	T12643	punct	+,cre
R7962	T12648	T12641	amod	transgenic,males
R7963	T12649	T12632	punct	", ",mated
R7964	T12650	T12632	cc	and,mated
R7965	T12651	T12652	nsubjpass	blastocysts,flushed
R7966	T12652	T12632	conj	flushed,mated
R7967	T12653	T12652	auxpass	were,flushed
R7968	T12654	T12652	prep	from,flushed
R7969	T12655	T12656	amod	uterine,horns
R797	T1305	T1303	nmod	stem,cells
R7970	T12656	T12654	pobj	horns,from
R7971	T12657	T12652	prep	with,flushed
R7972	T12658	T12659	compound	M2,medium
R7973	T12659	T12657	pobj	medium,with
R7974	T12660	T12661	punct	(,Sigma
R7975	T12661	T12659	parataxis	Sigma,medium
R7976	T12662	T12661	punct	),Sigma
R7977	T12663	T12652	prep	at,flushed
R7978	T12664	T12665	nummod	3.5,dpc
R7979	T12665	T12663	pobj	dpc,at
R798	T1306	T1305	punct	(,stem
R7980	T12666	T12652	punct	.,flushed
R7981	T12668	T12669	amod	Individual,blastocysts
R7982	T12669	T12670	nsubjpass	blastocysts,cultured
R7983	T12671	T12670	auxpass	were,cultured
R7984	T12672	T12670	prep	in,cultured
R7985	T12673	T12674	amod	multiwell,cultures
R7986	T12674	T12676	compound	cultures,plates
R7987	T12675	T12674	compound	tissue,cultures
R7988	T12676	T12672	pobj	plates,in
R7989	T12677	T12678	mark	as,described
R799	T1307	T1305	appos	ES,stem
R7990	T12678	T12670	advcl	described,cultured
R7991	T12679	T12678	advmod	previously,described
R7992	T12680	T12681	punct	[,44
R7993	T12681	T12670	parataxis	44,cultured
R7994	T12682	T12681	punct	],44
R7995	T12683	T12670	punct	.,cultured
R7996	T12685	T12686	nsubj	Cultures,inspected
R7997	T12687	T12686	aux	were,inspected
R7998	T12688	T12686	cc	and,inspected
R7999	T12689	T12686	conj	photographed,inspected
R8	T162	T161	prep	of,Pattern
R80	T241	T242	compound	alpha,thalassemia
R800	T1308	T1303	punct	),cells
R8000	T12690	T12686	advmod	daily,inspected
R8001	T12691	T12686	cc	and,inspected
R8002	T12692	T12693	det	the,extent
R8003	T12693	T12694	nsubj	extent,scored
R8004	T12694	T12686	conj	scored,inspected
R8005	T12695	T12693	prep	of,extent
R8006	T12696	T12695	pobj	outgrowth,of
R8007	T12697	T12694	punct	.,scored
R8008	T12699	T12700	prep	After,harvested
R8009	T12701	T12702	nummod	7,d
R801	T1309	T1282	punct	", ",generated
R8010	T12702	T12699	pobj	d,After
R8011	T12703	T12700	punct	", ",harvested
R8012	T12704	T12700	nsubjpass	cultures,harvested
R8013	T12705	T12700	auxpass	were,harvested
R8014	T12706	T12700	cc	and,harvested
R8015	T12707	T12708	nsubj	DNA,extracted
R8016	T12708	T12700	conj	extracted,harvested
R8017	T12709	T12700	punct	.,harvested
R8018	T12711	T12712	det	The,genotype
R8019	T12712	T12714	nsubjpass	genotype,determined
R802	T1310	T1282	cc	and,generated
R8020	T12713	T12712	compound	Atrx,genotype
R8021	T12715	T12712	cc	and,genotype
R8022	T12716	T12712	conj	sex,genotype
R8023	T12717	T12712	prep	of,genotype
R8024	T12718	T12719	det	each,culture
R8025	T12719	T12717	pobj	culture,of
R8026	T12720	T12714	auxpass	was,determined
R8027	T12721	T12714	prep	by,determined
R8028	T12722	T12721	pobj	PCR,by
R8029	T12723	T12724	mark	as,described
R803	T1311	T1282	conj	used,generated
R8030	T12724	T12714	advcl	described,determined
R8031	T12725	T12724	prep	in,described
R8032	T12726	T12727	compound	Protocol,S1
R8033	T12727	T12725	pobj	S1,in
R8034	T12728	T12714	punct	.,determined
R804	T1312	T1313	det	these,cells
R805	T1313	T1311	dobj	cells,used
R806	T1314	T1315	aux	to,examine
R807	T1315	T1311	advcl	examine,used
R808	T1316	T1317	det	the,effect
R809	T1317	T1315	dobj	effect,examine
R81	T242	T238	appos	thalassemia,X
R810	T1318	T1317	prep	of,effect
R811	T1319	T1320	amod	ablating,expression
R812	T1320	T1318	pobj	expression,of
R813	T1321	T1320	prep	of,expression
R814	T1322	T1323	det	the,protein
R815	T1323	T1321	pobj	protein,of
R816	T1324	T1325	amod	full,length
R817	T1325	T1323	compound	length,protein
R818	T1326	T1325	punct	-,length
R819	T1327	T1323	compound	Atrx,protein
R82	T243	T244	amod	mental,retardation
R820	T1328	T1317	prep	in,effect
R821	T1329	T1330	compound	ES,cells
R822	T1330	T1328	pobj	cells,in
R823	T1331	T1328	cc	and,in
R824	T1332	T1328	conj	in,in
R825	T1333	T1334	compound	mouse,embryos
R826	T1334	T1332	pobj	embryos,in
R827	T1335	T1282	punct	.,generated
R83	T244	T242	appos	retardation,thalassemia
R830	T1575	T1574	prep	of,Generation
R831	T1576	T1577	compound	ES,Cells
R832	T1577	T1575	pobj	Cells,of
R833	T1578	T1577	acl	Lacking,Cells
R834	T1579	T1580	amod	Full,Length
R835	T1580	T1582	compound	Length,Atrx
R836	T1581	T1580	punct	-,Length
R837	T1582	T1578	dobj	Atrx,Lacking
R838	T1584	T1585	prep	Like,is
R839	T1586	T1587	det	the,gene
R84	T245	T242	punct	", ",thalassemia
R840	T1587	T1584	pobj	gene,Like
R841	T1588	T1587	amod	human,gene
R842	T1589	T1585	punct	", ",is
R843	T1590	T1591	det	the,gene
R844	T1591	T1585	nsubj	gene,is
R845	T1592	T1591	compound	mouse,gene
R846	T1593	T1591	compound	Atrx,gene
R847	T1594	T1585	advmod	also,is
R848	T1595	T1596	npadvmod	X,linked
R849	T1596	T1585	acomp	linked,is
R85	T246	T247	npadvmod	X,linked
R850	T1597	T1596	punct	-,linked
R851	T1598	T1585	punct	", ",is
R852	T1599	T1600	amod	such,give
R853	T1600	T1585	advcl	give,is
R854	T1601	T1600	mark	that,give
R855	T1602	T1603	det	a,disruption
R856	T1603	T1600	nsubj	disruption,give
R857	T1604	T1603	amod	direct,disruption
R858	T1605	T1603	prep	of,disruption
R859	T1606	T1607	det	the,allele
R86	T247	T242	amod	linked,thalassemia
R860	T1607	T1605	pobj	allele,of
R861	T1608	T1607	amod	single,allele
R862	T1609	T1607	compound	Atrx,allele
R863	T1610	T1603	prep	in,disruption
R864	T1611	T1612	amod	male,cells
R865	T1612	T1610	pobj	cells,in
R866	T1613	T1612	compound	ES,cells
R867	T1614	T1600	aux	would,give
R868	T1615	T1600	advmod	immediately,give
R869	T1616	T1600	dobj	rise,give
R87	T248	T247	punct	-,linked
R870	T1617	T1600	prep	to,give
R871	T1618	T1619	det	the,state
R872	T1619	T1617	pobj	state,to
R873	T1620	T1619	amod	null,state
R874	T1621	T1585	punct	.,is
R875	T1623	T1624	det	No,clones
R876	T1624	T1626	nsubjpass	clones,recovered
R877	T1625	T1624	amod	targeted,clones
R878	T1627	T1626	auxpass	were,recovered
R879	T1628	T1626	prep	after,recovered
R88	T249	T236	punct	),syndrome
R880	T1629	T1630	amod	attempted,recombination
R881	T1630	T1628	pobj	recombination,after
R882	T1631	T1630	amod	homologous,recombination
R883	T1632	T1630	prep	in,recombination
R884	T1633	T1634	amod	male,cells
R885	T1634	T1632	pobj	cells,in
R886	T1635	T1636	compound	E14TG2a,ES
R887	T1636	T1634	compound	ES,cells
R888	T1637	T1626	advcl	using,recovered
R889	T1638	T1639	nummod	two,vectors
R89	T250	T223	punct	", ",inactivated
R890	T1639	T1637	dobj	vectors,using
R891	T1640	T1639	amod	different,vectors
R892	T1641	T1642	dep	that,removed
R893	T1642	T1639	relcl	removed,vectors
R894	T1643	T1642	dobj	exon,removed
R895	T1644	T1643	nummod	18,exon
R896	T1645	T1643	prep	of,exon
R897	T1646	T1647	det	the,gene
R898	T1647	T1645	pobj	gene,of
R899	T1648	T1647	compound	Atrx,gene
R9	T163	T164	compound	X,Inactivation
R90	T251	T223	nsubj	we,inactivated
R900	T1649	T1626	punct	.,recovered
R901	T1651	T1652	nsubj	Exon,encodes
R902	T1652	T1654	ccomp	encodes,shown
R903	T1653	T1651	nummod	18,Exon
R904	T1655	T1656	det	the,first
R905	T1656	T1652	dobj	first,encodes
R906	T1657	T1656	prep	of,first
R907	T1658	T1659	det	the,motifs
R908	T1659	T1657	pobj	motifs,of
R909	T1660	T1659	nummod	seven,motifs
R91	T252	T223	advmod	conditionally,inactivated
R910	T1661	T1656	acl	composing,first
R911	T1662	T1663	det	the,domain
R912	T1663	T1661	dobj	domain,composing
R913	T1664	T1663	amod	conserved,domain
R914	T1665	T1666	npadvmod	SNF2,like
R915	T1666	T1663	amod	like,domain
R916	T1667	T1666	punct	-,like
R917	T1668	T1663	prep	of,domain
R918	T1669	T1668	pobj	Atrx,of
R919	T1670	T1671	punct	(,Figure
R92	T253	T254	det	the,homolog
R920	T1671	T1652	parataxis	Figure,encodes
R921	T1672	T1671	nummod	1,Figure
R922	T1673	T1671	punct	),Figure
R923	T1674	T1654	punct	;,shown
R924	T1675	T1654	nsubjpass	mutation,shown
R925	T1676	T1675	prep	of,mutation
R926	T1677	T1678	det	the,motif
R927	T1678	T1676	pobj	motif,of
R928	T1679	T1678	amod	corresponding,motif
R929	T1680	T1678	prep	of,motif
R93	T254	T223	dobj	homolog,inactivated
R930	T1681	T1682	det	the,protein
R931	T1682	T1680	pobj	protein,of
R932	T1683	T1682	compound	yeast,protein
R933	T1684	T1682	compound	SNF2,protein
R934	T1685	T1654	aux	has,shown
R935	T1686	T1654	auxpass	been,shown
R936	T1687	T1688	aux	to,impair
R937	T1688	T1654	xcomp	impair,shown
R938	T1689	T1688	advmod	severely,impair
R939	T1690	T1691	compound	SWI,SNF
R94	T255	T254	prep	in,homolog
R940	T1691	T1693	npadvmod	SNF,dependent
R941	T1692	T1691	punct	/,SNF
R942	T1693	T1695	amod	dependent,expression
R943	T1694	T1693	punct	-,dependent
R944	T1695	T1688	dobj	expression,impair
R945	T1696	T1695	compound	gene,expression
R946	T1697	T1698	punct	[,12
R947	T1698	T1654	parataxis	12,shown
R948	T1699	T1698	punct	],12
R949	T1700	T1654	punct	.,shown
R95	T256	T255	pobj	mice,in
R950	T1702	T1703	det	The,failure
R951	T1703	T1704	nsubj	failure,suggested
R952	T1705	T1706	aux	to,recover
R953	T1706	T1703	acl	recover,failure
R954	T1707	T1708	amod	targeted,clones
R955	T1708	T1706	dobj	clones,recover
R956	T1709	T1706	prep	with,recover
R957	T1710	T1711	det	these,vectors
R958	T1711	T1709	pobj	vectors,with
R959	T1712	T1713	mark	that,be
R96	T257	T254	punct	", ",homolog
R960	T1713	T1704	advcl	be,suggested
R961	T1714	T1713	nsubj	Atrx,be
R962	T1715	T1713	aux	may,be
R963	T1716	T1713	acomp	important,be
R964	T1717	T1716	prep	for,important
R965	T1718	T1719	amod	normal,cell
R966	T1719	T1717	pobj	cell,for
R967	T1720	T1719	compound	ES,cell
R968	T1721	T1719	appos	growth,cell
R969	T1722	T1721	cc	and,growth
R97	T258	T254	appos	Atrx,homolog
R970	T1723	T1721	conj	expansion,growth
R971	T1724	T1713	cc	and,be
R972	T1725	T1726	mark	that,be
R973	T1726	T1713	conj	be,be
R974	T1727	T1728	amod	direct,targeting
R975	T1728	T1726	nsubj	targeting,be
R976	T1729	T1728	prep	of,targeting
R977	T1730	T1731	det	the,locus
R978	T1731	T1729	pobj	locus,of
R979	T1732	T1731	amod	single,locus
R98	T259	T223	punct	", ",inactivated
R980	T1733	T1726	aux	may,be
R981	T1734	T1726	neg	not,be
R982	T1735	T1726	acomp	possible,be
R983	T1736	T1704	punct	.,suggested
R984	T1738	T1739	nsubj	We,adopted
R985	T1740	T1739	advmod	therefore,adopted
R986	T1741	T1742	det	a,strategy
R987	T1742	T1739	dobj	strategy,adopted
R988	T1743	T1742	amod	conditional,strategy
R989	T1744	T1742	prep	for,strategy
R99	T260	T223	prep	at,inactivated
R990	T1745	T1744	pcomp	targeting,for
R991	T1746	T1745	dobj	exon,targeting
R992	T1747	T1746	nummod	18,exon
R993	T1748	T1749	punct	(,Figure
R994	T1749	T1739	parataxis	Figure,adopted
R995	T1750	T1749	nummod	2,Figure
R996	T1751	T1749	punct	),Figure
R997	T1752	T1739	cc	and,adopted
R998	T1753	T1739	conj	recovered,adopted
R999	T1754	T1755	nummod	two,clones

PMC:1440874 JSONTXT 8 Projects

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PMC:1440874 JSON TXT 8 Projects