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PMC:1435744 / 46186-47109 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T27028 0-11 JJ denotes Subcellular
T27030 12-24 NN denotes localization
T27029 25-33 NN denotes analysis
T27031 33-309 sentence denotes We transfected the plasmid encoding HA-tagged full-length mr-s into HEK293T cells, seeded cells on coverslips coated with collagen 48 hr after transfection, fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) at room temperature for 20 min, and washed with PBS.
T27032 34-36 PRP denotes We
T27033 37-48 VBD denotes transfected
T27034 49-52 DT denotes the
T27035 53-60 NN denotes plasmid
T27036 61-69 VBG denotes encoding
T27037 70-72 NN denotes HA
T27039 72-73 HYPH denotes -
T27038 73-79 VBN denotes tagged
T27041 80-84 JJ denotes full
T27043 84-85 HYPH denotes -
T27042 85-91 NN denotes length
T27044 92-94 NN denotes mr
T27045 94-95 HYPH denotes -
T27040 95-96 NN denotes s
T27046 97-101 IN denotes into
T27047 102-109 NN denotes HEK293T
T27048 110-115 NNS denotes cells
T27049 115-117 , denotes ,
T27050 117-123 VBD denotes seeded
T27051 124-129 NNS denotes cells
T27052 130-132 IN denotes on
T27053 133-143 NNS denotes coverslips
T27054 144-150 VBN denotes coated
T27055 151-155 IN denotes with
T27056 156-164 NN denotes collagen
T27057 165-167 CD denotes 48
T27058 168-170 NN denotes hr
T27059 171-176 IN denotes after
T27060 177-189 NN denotes transfection
T27061 189-191 , denotes ,
T27062 191-196 VBN denotes fixed
T27063 197-201 IN denotes with
T27064 202-203 CD denotes 4
T27065 203-204 NN denotes %
T27066 205-221 NN denotes paraformaldehyde
T27067 222-224 IN denotes in
T27068 225-234 NN denotes phosphate
T27070 234-235 HYPH denotes -
T27069 235-243 VBN denotes buffered
T27071 244-250 NN denotes saline
T27072 251-252 -LRB- denotes (
T27073 252-255 NN denotes PBS
T27074 255-256 -RRB- denotes )
T27075 257-259 IN denotes at
T27076 260-264 NN denotes room
T27077 265-276 NN denotes temperature
T27078 277-280 IN denotes for
T27079 281-283 CD denotes 20
T27080 284-287 NN denotes min
T27081 287-289 , denotes ,
T27082 289-292 CC denotes and
T27083 293-299 VBN denotes washed
T27084 300-304 IN denotes with
T27085 305-308 NN denotes PBS
T27086 308-309 . denotes .
T27087 309-504 sentence denotes Then we permeabilized cells in PBS containing 0.1% Triton X-100 for 15 min, washed again with PBS, and incubated in PBS containing 0.1% bovine serum albumin (BSA) for 30 min at room temperature.
T27088 310-314 RB denotes Then
T27090 315-317 PRP denotes we
T27089 318-331 VBD denotes permeabilized
T27091 332-337 NNS denotes cells
T27092 338-340 IN denotes in
T27093 341-344 NN denotes PBS
T27094 345-355 VBG denotes containing
T27095 356-359 CD denotes 0.1
T27096 359-360 NN denotes %
T27098 361-367 NN denotes Triton
T27099 368-369 NN denotes X
T27100 369-370 HYPH denotes -
T27097 370-373 NN denotes 100
T27101 374-377 IN denotes for
T27102 378-380 CD denotes 15
T27103 381-384 NN denotes min
T27104 384-386 , denotes ,
T27105 386-392 VBD denotes washed
T27106 393-398 RB denotes again
T27107 399-403 IN denotes with
T27108 404-407 NN denotes PBS
T27109 407-409 , denotes ,
T27110 409-412 CC denotes and
T27111 413-422 VBD denotes incubated
T27112 423-425 IN denotes in
T27113 426-429 NN denotes PBS
T27114 430-440 VBG denotes containing
T27115 441-444 CD denotes 0.1
T27116 444-445 NN denotes %
T27117 446-452 JJ denotes bovine
T27119 453-458 NN denotes serum
T27118 459-466 NN denotes albumin
T27120 467-468 -LRB- denotes (
T27121 468-471 NN denotes BSA
T27122 471-472 -RRB- denotes )
T27123 473-476 IN denotes for
T27124 477-479 CD denotes 30
T27125 480-483 NN denotes min
T27126 484-486 IN denotes at
T27127 487-491 NN denotes room
T27128 492-503 NN denotes temperature
T27129 503-504 . denotes .
T27130 504-598 sentence denotes We incubated cells with anti-HA antibody overnight at 4°C (1:200 in PBS containing 0.2% BSA).
T27131 505-507 PRP denotes We
T27132 508-517 VBD denotes incubated
T27133 518-523 NNS denotes cells
T27134 524-528 IN denotes with
T27135 529-536 JJ denotes anti-HA
T27136 537-545 NN denotes antibody
T27137 546-555 RB denotes overnight
T27138 556-558 IN denotes at
T27139 559-560 CD denotes 4
T27140 560-562 NN denotes °C
T27141 563-564 -LRB- denotes (
T27142 564-565 CD denotes 1
T27143 565-566 SYM denotes :
T27144 566-569 CD denotes 200
T27145 570-572 IN denotes in
T27146 573-576 NN denotes PBS
T27147 577-587 VBG denotes containing
T27148 588-591 CD denotes 0.2
T27149 591-592 NN denotes %
T27150 593-596 NN denotes BSA
T27151 596-597 -RRB- denotes )
T27152 597-598 . denotes .
T27153 598-781 sentence denotes The following day, we washed cells three times with PBS and incubated with a Cy3-conjugated goat IgG against rabbit IgG (1:400 in PBS, Jackson ImmunoReseach Laboratories) for 30 min.
T27154 599-602 DT denotes The
T27156 603-612 VBG denotes following
T27155 613-616 NN denotes day
T27158 616-618 , denotes ,
T27159 618-620 PRP denotes we
T27157 621-627 VBD denotes washed
T27160 628-633 NNS denotes cells
T27161 634-639 CD denotes three
T27162 640-645 NNS denotes times
T27163 646-650 IN denotes with
T27164 651-654 NN denotes PBS
T27165 655-658 CC denotes and
T27166 659-668 VBD denotes incubated
T27167 669-673 IN denotes with
T27168 674-675 DT denotes a
T27170 676-679 NN denotes Cy3
T27172 679-680 HYPH denotes -
T27171 680-690 VBN denotes conjugated
T27173 691-695 NN denotes goat
T27169 696-699 NN denotes IgG
T27174 700-707 IN denotes against
T27175 708-714 NN denotes rabbit
T27176 715-718 NN denotes IgG
T27177 719-720 -LRB- denotes (
T27179 720-721 CD denotes 1
T27180 721-722 SYM denotes :
T27181 722-725 CD denotes 400
T27182 726-728 IN denotes in
T27183 729-732 NN denotes PBS
T27184 732-734 , denotes ,
T27185 734-741 NNP denotes Jackson
T27186 742-755 NNP denotes ImmunoReseach
T27178 756-768 NNP denotes Laboratories
T27187 768-769 -RRB- denotes )
T27188 770-773 IN denotes for
T27189 774-776 CD denotes 30
T27190 777-780 NN denotes min
T27191 780-781 . denotes .
T27192 781-923 sentence denotes We rinsed cells three times with PBS, followed by observation using a confocal microscope FV300 (Olympus) equipped with a 60× objective lens.
T27193 782-784 PRP denotes We
T27194 785-791 VBD denotes rinsed
T27195 792-797 NNS denotes cells
T27196 798-803 CD denotes three
T27197 804-809 NNS denotes times
T27198 810-814 IN denotes with
T27199 815-818 NN denotes PBS
T27200 818-820 , denotes ,
T27201 820-828 VBN denotes followed
T27202 829-831 IN denotes by
T27203 832-843 NN denotes observation
T27204 844-849 VBG denotes using
T27205 850-851 DT denotes a
T27207 852-860 JJ denotes confocal
T27208 861-871 NN denotes microscope
T27206 872-877 NN denotes FV300
T27209 878-879 -LRB- denotes (
T27210 879-886 NNP denotes Olympus
T27211 886-887 -RRB- denotes )
T27212 888-896 VBN denotes equipped
T27213 897-901 IN denotes with
T27214 902-903 DT denotes a
T27216 904-906 CD denotes 60
T27217 906-907 SYM denotes ×
T27218 908-917 NN denotes objective
T27215 918-922 NN denotes lens
T27219 922-923 . denotes .
R7687 T27028 T27029 amod Subcellular,analysis
R7688 T27030 T27029 compound localization,analysis
R7689 T27032 T27033 nsubj We,transfected
R7690 T27034 T27035 det the,plasmid
R7691 T27035 T27033 dobj plasmid,transfected
R7692 T27036 T27035 acl encoding,plasmid
R7693 T27037 T27038 npadvmod HA,tagged
R7694 T27038 T27040 amod tagged,s
R7695 T27039 T27038 punct -,tagged
R7696 T27040 T27036 dobj s,encoding
R7697 T27041 T27042 amod full,length
R7698 T27042 T27040 compound length,s
R7699 T27043 T27042 punct -,length
R7700 T27044 T27040 compound mr,s
R7701 T27045 T27040 punct -,s
R7702 T27046 T27033 prep into,transfected
R7703 T27047 T27048 compound HEK293T,cells
R7704 T27048 T27046 pobj cells,into
R7705 T27049 T27033 punct ", ",transfected
R7706 T27050 T27033 conj seeded,transfected
R7707 T27051 T27050 dobj cells,seeded
R7708 T27052 T27050 prep on,seeded
R7709 T27053 T27052 pobj coverslips,on
R7710 T27054 T27053 acl coated,coverslips
R7711 T27055 T27054 prep with,coated
R7712 T27056 T27055 pobj collagen,with
R7713 T27057 T27058 nummod 48,hr
R7714 T27058 T27059 npadvmod hr,after
R7715 T27059 T27050 prep after,seeded
R7716 T27060 T27059 pobj transfection,after
R7717 T27061 T27050 punct ", ",seeded
R7718 T27062 T27050 conj fixed,seeded
R7719 T27063 T27062 prep with,fixed
R7720 T27064 T27065 nummod 4,%
R7721 T27065 T27066 compound %,paraformaldehyde
R7722 T27066 T27063 pobj paraformaldehyde,with
R7723 T27067 T27066 prep in,paraformaldehyde
R7724 T27068 T27069 npadvmod phosphate,buffered
R7725 T27069 T27071 amod buffered,saline
R7726 T27070 T27069 punct -,buffered
R7727 T27071 T27067 pobj saline,in
R7728 T27072 T27071 punct (,saline
R7729 T27073 T27071 appos PBS,saline
R7730 T27074 T27062 punct ),fixed
R7731 T27075 T27062 prep at,fixed
R7732 T27076 T27077 compound room,temperature
R7733 T27077 T27075 pobj temperature,at
R7734 T27078 T27062 prep for,fixed
R7735 T27079 T27080 nummod 20,min
R7736 T27080 T27078 pobj min,for
R7737 T27081 T27062 punct ", ",fixed
R7738 T27082 T27062 cc and,fixed
R7739 T27083 T27062 conj washed,fixed
R7740 T27084 T27083 prep with,washed
R7741 T27085 T27084 pobj PBS,with
R7742 T27086 T27033 punct .,transfected
R7743 T27088 T27089 advmod Then,permeabilized
R7744 T27090 T27089 nsubj we,permeabilized
R7745 T27091 T27089 dobj cells,permeabilized
R7746 T27092 T27089 prep in,permeabilized
R7747 T27093 T27092 pobj PBS,in
R7748 T27094 T27093 acl containing,PBS
R7749 T27095 T27096 nummod 0.1,%
R7750 T27096 T27097 compound %,100
R7751 T27097 T27094 dobj 100,containing
R7752 T27098 T27097 compound Triton,100
R7753 T27099 T27097 compound X,100
R7754 T27100 T27097 punct -,100
R7755 T27101 T27089 prep for,permeabilized
R7756 T27102 T27103 nummod 15,min
R7757 T27103 T27101 pobj min,for
R7758 T27104 T27089 punct ", ",permeabilized
R7759 T27105 T27089 conj washed,permeabilized
R7760 T27106 T27105 advmod again,washed
R7761 T27107 T27105 prep with,washed
R7762 T27108 T27107 pobj PBS,with
R7763 T27109 T27105 punct ", ",washed
R7764 T27110 T27105 cc and,washed
R7765 T27111 T27105 conj incubated,washed
R7766 T27112 T27111 prep in,incubated
R7767 T27113 T27112 pobj PBS,in
R7768 T27114 T27113 acl containing,PBS
R7769 T27115 T27116 nummod 0.1,%
R7770 T27116 T27114 dobj %,containing
R7771 T27117 T27118 amod bovine,albumin
R7772 T27118 T27116 appos albumin,%
R7773 T27119 T27118 compound serum,albumin
R7774 T27120 T27118 punct (,albumin
R7775 T27121 T27118 appos BSA,albumin
R7776 T27122 T27111 punct ),incubated
R7777 T27123 T27111 prep for,incubated
R7778 T27124 T27125 nummod 30,min
R7779 T27125 T27123 pobj min,for
R7780 T27126 T27111 prep at,incubated
R7781 T27127 T27128 compound room,temperature
R7782 T27128 T27126 pobj temperature,at
R7783 T27129 T27089 punct .,permeabilized
R7784 T27131 T27132 nsubj We,incubated
R7785 T27133 T27132 dobj cells,incubated
R7786 T27134 T27132 prep with,incubated
R7787 T27135 T27136 amod anti-HA,antibody
R7788 T27136 T27134 pobj antibody,with
R7789 T27137 T27132 advmod overnight,incubated
R7790 T27138 T27132 prep at,incubated
R7791 T27139 T27140 nummod 4,°C
R7792 T27140 T27138 pobj °C,at
R7793 T27141 T27142 punct (,1
R7794 T27142 T27132 parataxis 1,incubated
R7795 T27143 T27144 punct :,200
R7796 T27144 T27142 prep 200,1
R7797 T27145 T27142 prep in,1
R7798 T27146 T27145 pobj PBS,in
R7799 T27147 T27146 acl containing,PBS
R7800 T27148 T27149 nummod 0.2,%
R7801 T27149 T27150 compound %,BSA
R7802 T27150 T27147 dobj BSA,containing
R7803 T27151 T27142 punct ),1
R7804 T27152 T27132 punct .,incubated
R7805 T27154 T27155 det The,day
R7806 T27155 T27157 npadvmod day,washed
R7807 T27156 T27155 amod following,day
R7808 T27158 T27157 punct ", ",washed
R7809 T27159 T27157 nsubj we,washed
R7810 T27160 T27157 dobj cells,washed
R7811 T27161 T27162 nummod three,times
R7812 T27162 T27157 npadvmod times,washed
R7813 T27163 T27157 prep with,washed
R7814 T27164 T27163 pobj PBS,with
R7815 T27165 T27157 cc and,washed
R7816 T27166 T27157 conj incubated,washed
R7817 T27167 T27166 prep with,incubated
R7818 T27168 T27169 det a,IgG
R7819 T27169 T27167 pobj IgG,with
R7820 T27170 T27171 npadvmod Cy3,conjugated
R7821 T27171 T27169 amod conjugated,IgG
R7822 T27172 T27171 punct -,conjugated
R7823 T27173 T27169 compound goat,IgG
R7824 T27174 T27169 prep against,IgG
R7825 T27175 T27176 compound rabbit,IgG
R7826 T27176 T27174 pobj IgG,against
R7827 T27177 T27178 punct (,Laboratories
R7828 T27178 T27169 parataxis Laboratories,IgG
R7829 T27179 T27178 dep 1,Laboratories
R7830 T27180 T27181 punct :,400
R7831 T27181 T27179 prep 400,1
R7832 T27182 T27179 prep in,1
R7833 T27183 T27182 pobj PBS,in
R7834 T27184 T27178 punct ", ",Laboratories
R7835 T27185 T27178 compound Jackson,Laboratories
R7836 T27186 T27178 compound ImmunoReseach,Laboratories
R7837 T27187 T27178 punct ),Laboratories
R7838 T27188 T27166 prep for,incubated
R7839 T27189 T27190 nummod 30,min
R7840 T27190 T27188 pobj min,for
R7841 T27191 T27157 punct .,washed
R7842 T27193 T27194 nsubj We,rinsed
R7843 T27195 T27194 dobj cells,rinsed
R7844 T27196 T27197 nummod three,times
R7845 T27197 T27194 npadvmod times,rinsed
R7846 T27198 T27194 prep with,rinsed
R7847 T27199 T27198 pobj PBS,with
R7848 T27200 T27194 punct ", ",rinsed
R7849 T27201 T27194 advcl followed,rinsed
R7850 T27202 T27201 agent by,followed
R7851 T27203 T27202 pobj observation,by
R7852 T27204 T27203 acl using,observation
R7853 T27205 T27206 det a,FV300
R7854 T27206 T27204 dobj FV300,using
R7855 T27207 T27206 amod confocal,FV300
R7856 T27208 T27206 compound microscope,FV300
R7857 T27209 T27210 punct (,Olympus
R7858 T27210 T27206 parataxis Olympus,FV300
R7859 T27211 T27210 punct ),Olympus
R7860 T27212 T27206 acl equipped,FV300
R7861 T27213 T27212 prep with,equipped
R7862 T27214 T27215 det a,lens
R7863 T27215 T27213 pobj lens,with
R7864 T27216 T27215 nummod 60,lens
R7865 T27217 T27216 punct ×,60
R7866 T27218 T27215 compound objective,lens
R7867 T27219 T27194 punct .,rinsed