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PMC:1435744 / 42724-43281 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T24536 0-2 NN denotes RT
T24538 2-3 HYPH denotes -
T24537 3-6 NN denotes PCR
T24539 7-15 NN denotes analysis
T24540 15-69 sentence denotes Total RNA was isolated from each tissue using Trizol.
T24541 16-21 JJ denotes Total
T24542 22-25 NN denotes RNA
T24544 26-29 VBD denotes was
T24543 30-38 VBN denotes isolated
T24545 39-43 IN denotes from
T24546 44-48 DT denotes each
T24547 49-55 NN denotes tissue
T24548 56-61 VBG denotes using
T24549 62-68 NN denotes Trizol
T24550 68-69 . denotes .
T24551 69-147 sentence denotes A 1 μg of total RNA was reverse transcribed using SuperscriptII (Invitrogen).
T24552 70-71 DT denotes A
T24554 72-73 CD denotes 1
T24553 74-76 NN denotes μg
T24556 77-79 IN denotes of
T24557 80-85 JJ denotes total
T24558 86-89 NN denotes RNA
T24559 90-93 VBD denotes was
T24560 94-101 JJ denotes reverse
T24555 102-113 VBN denotes transcribed
T24561 114-119 VBG denotes using
T24562 120-133 NNP denotes SuperscriptII
T24563 134-135 -LRB- denotes (
T24564 135-145 NNP denotes Invitrogen
T24565 145-146 -RRB- denotes )
T24566 146-147 . denotes .
T24567 147-288 sentence denotes RT-PCR primers, which span introns, for detection of mr-s cDNA were 5'-TGTCCAGCCCAGCCAACCCAAGGAGACGACA-3' and 5'-TGTGGTCTCCTCATCAGTGAAGA-3'.
T24568 148-150 NN denotes RT
T24570 150-151 HYPH denotes -
T24569 151-154 NN denotes PCR
T24571 155-162 NNS denotes primers
T24573 162-164 , denotes ,
T24574 164-169 WDT denotes which
T24575 170-174 VBP denotes span
T24576 175-182 NNS denotes introns
T24577 182-184 , denotes ,
T24578 184-187 IN denotes for
T24579 188-197 NN denotes detection
T24580 198-200 IN denotes of
T24581 201-203 NN denotes mr
T24583 203-204 HYPH denotes -
T24582 204-205 NN denotes s
T24584 206-210 NN denotes cDNA
T24572 211-215 VBD denotes were
T24585 216-217 CD denotes 5
T24587 217-218 SYM denotes '
T24588 218-219 HYPH denotes -
T24586 219-250 NN denotes TGTCCAGCCCAGCCAACCCAAGGAGACGACA
T24589 250-251 HYPH denotes -
T24590 251-252 CD denotes 3
T24591 252-253 SYM denotes '
T24592 254-257 CC denotes and
T24593 258-259 CD denotes 5
T24595 259-260 SYM denotes '
T24596 260-261 HYPH denotes -
T24594 261-284 NN denotes TGTGGTCTCCTCATCAGTGAAGA
T24597 284-285 HYPH denotes -
T24598 285-286 CD denotes 3
T24599 286-287 SYM denotes '
T24600 287-288 . denotes .
T24601 288-372 sentence denotes Product size was 292 bp (positions 965–1256 of Genbank Accession Number #AY458844).
T24602 289-296 NN denotes Product
T24603 297-301 NN denotes size
T24604 302-305 VBD denotes was
T24605 306-309 CD denotes 292
T24606 310-312 NN denotes bp
T24607 313-314 -LRB- denotes (
T24609 314-323 NNS denotes positions
T24608 324-327 CD denotes 965
T24610 327-328 SYM denotes –
T24611 328-332 CD denotes 1256
T24612 333-335 IN denotes of
T24613 336-343 NNP denotes Genbank
T24615 344-353 NNP denotes Accession
T24616 354-360 NNP denotes Number
T24617 361-362 SYM denotes #
T24614 362-370 NN denotes AY458844
T24618 370-371 -RRB- denotes )
T24619 371-372 . denotes .
T24620 372-557 sentence denotes Primer pairs for mouse G3PDH were 5'-ACCACAGTCCATGCCATCAC-3' and 5'-TCCACCACCCTGTTGCTGTA- 3' which amplified a 452-bp product (positions 587–1038 of Genbank Accession Number #BC85275).
T24621 373-379 NN denotes Primer
T24622 380-385 NNS denotes pairs
T24624 386-389 IN denotes for
T24625 390-395 NN denotes mouse
T24626 396-401 NN denotes G3PDH
T24623 402-406 VBD denotes were
T24627 407-408 CD denotes 5
T24629 408-409 SYM denotes '
T24630 409-410 HYPH denotes -
T24628 410-430 NN denotes ACCACAGTCCATGCCATCAC
T24631 430-431 HYPH denotes -
T24632 431-432 CD denotes 3
T24633 432-433 SYM denotes '
T24634 434-437 CC denotes and
T24635 438-439 CD denotes 5
T24637 439-440 SYM denotes '
T24638 440-441 HYPH denotes -
T24636 441-461 NN denotes TCCACCACCCTGTTGCTGTA
T24639 461-462 HYPH denotes -
T24640 463-464 CD denotes 3
T24641 464-465 SYM denotes '
T24642 466-471 WDT denotes which
T24643 472-481 VBD denotes amplified
T24644 482-483 DT denotes a
T24646 484-487 CD denotes 452
T24648 487-488 HYPH denotes -
T24647 488-490 NN denotes bp
T24645 491-498 NN denotes product
T24649 499-500 -LRB- denotes (
T24651 500-509 NNS denotes positions
T24650 510-513 CD denotes 587
T24652 513-514 SYM denotes –
T24653 514-518 CD denotes 1038
T24654 519-521 IN denotes of
T24655 522-529 NNP denotes Genbank
T24657 530-539 NN denotes Accession
T24658 540-546 NN denotes Number
T24659 547-548 SYM denotes #
T24656 548-555 NN denotes BC85275
T24660 555-556 -RRB- denotes )
T24661 556-557 . denotes .
R6973 T24536 T24537 compound RT,PCR
R6974 T24537 T24539 compound PCR,analysis
R6975 T24538 T24537 punct -,PCR
R6976 T24541 T24542 amod Total,RNA
R6977 T24542 T24543 nsubjpass RNA,isolated
R6978 T24544 T24543 auxpass was,isolated
R6979 T24545 T24543 prep from,isolated
R6980 T24546 T24547 det each,tissue
R6981 T24547 T24545 pobj tissue,from
R6982 T24548 T24543 advcl using,isolated
R6983 T24549 T24548 dobj Trizol,using
R6984 T24550 T24543 punct .,isolated
R6985 T24552 T24553 det A,μg
R6986 T24553 T24555 nsubjpass μg,transcribed
R6987 T24554 T24553 nummod 1,μg
R6988 T24556 T24553 prep of,μg
R6989 T24557 T24558 amod total,RNA
R6990 T24558 T24556 pobj RNA,of
R6991 T24559 T24555 auxpass was,transcribed
R6992 T24560 T24555 amod reverse,transcribed
R6993 T24561 T24555 advcl using,transcribed
R6994 T24562 T24561 dobj SuperscriptII,using
R6995 T24563 T24564 punct (,Invitrogen
R6996 T24564 T24562 parataxis Invitrogen,SuperscriptII
R6997 T24565 T24564 punct ),Invitrogen
R6998 T24566 T24555 punct .,transcribed
R6999 T24568 T24569 compound RT,PCR
R7000 T24569 T24571 compound PCR,primers
R7001 T24570 T24569 punct -,PCR
R7002 T24571 T24572 nsubj primers,were
R7003 T24573 T24571 punct ", ",primers
R7004 T24574 T24575 dep which,span
R7005 T24575 T24571 relcl span,primers
R7006 T24576 T24575 dobj introns,span
R7007 T24577 T24571 punct ", ",primers
R7008 T24578 T24571 prep for,primers
R7009 T24579 T24578 pobj detection,for
R7010 T24580 T24579 prep of,detection
R7011 T24581 T24582 compound mr,s
R7012 T24582 T24584 compound s,cDNA
R7013 T24583 T24582 punct -,s
R7014 T24584 T24580 pobj cDNA,of
R7015 T24585 T24586 nummod 5,TGTCCAGCCCAGCCAACCCAAGGAGACGACA
R7016 T24586 T24572 attr TGTCCAGCCCAGCCAACCCAAGGAGACGACA,were
R7017 T24587 T24585 punct ',5
R7018 T24588 T24586 punct -,TGTCCAGCCCAGCCAACCCAAGGAGACGACA
R7019 T24589 T24586 punct -,TGTCCAGCCCAGCCAACCCAAGGAGACGACA
R7020 T24590 T24586 nummod 3,TGTCCAGCCCAGCCAACCCAAGGAGACGACA
R7021 T24591 T24586 punct ',TGTCCAGCCCAGCCAACCCAAGGAGACGACA
R7022 T24592 T24586 cc and,TGTCCAGCCCAGCCAACCCAAGGAGACGACA
R7023 T24593 T24594 nummod 5,TGTGGTCTCCTCATCAGTGAAGA
R7024 T24594 T24586 conj TGTGGTCTCCTCATCAGTGAAGA,TGTCCAGCCCAGCCAACCCAAGGAGACGACA
R7025 T24595 T24593 punct ',5
R7026 T24596 T24594 punct -,TGTGGTCTCCTCATCAGTGAAGA
R7027 T24597 T24594 punct -,TGTGGTCTCCTCATCAGTGAAGA
R7028 T24598 T24594 nummod 3,TGTGGTCTCCTCATCAGTGAAGA
R7029 T24599 T24594 punct ',TGTGGTCTCCTCATCAGTGAAGA
R7030 T24600 T24572 punct .,were
R7031 T24602 T24603 compound Product,size
R7032 T24603 T24604 nsubj size,was
R7033 T24605 T24606 nummod 292,bp
R7034 T24606 T24604 attr bp,was
R7035 T24607 T24608 punct (,965
R7036 T24608 T24604 parataxis 965,was
R7037 T24609 T24608 nmod positions,965
R7038 T24610 T24611 punct –,1256
R7039 T24611 T24608 prep 1256,965
R7040 T24612 T24608 prep of,965
R7041 T24613 T24614 nmod Genbank,AY458844
R7042 T24614 T24612 pobj AY458844,of
R7043 T24615 T24614 nmod Accession,AY458844
R7044 T24616 T24614 nmod Number,AY458844
R7045 T24617 T24614 punct #,AY458844
R7046 T24618 T24608 punct ),965
R7047 T24619 T24604 punct .,was
R7048 T24621 T24622 compound Primer,pairs
R7049 T24622 T24623 nsubj pairs,were
R7050 T24624 T24622 prep for,pairs
R7051 T24625 T24626 compound mouse,G3PDH
R7052 T24626 T24624 pobj G3PDH,for
R7053 T24627 T24628 nummod 5,ACCACAGTCCATGCCATCAC
R7054 T24628 T24623 attr ACCACAGTCCATGCCATCAC,were
R7055 T24629 T24627 punct ',5
R7056 T24630 T24628 punct -,ACCACAGTCCATGCCATCAC
R7057 T24631 T24628 punct -,ACCACAGTCCATGCCATCAC
R7058 T24632 T24628 nummod 3,ACCACAGTCCATGCCATCAC
R7059 T24633 T24628 punct ',ACCACAGTCCATGCCATCAC
R7060 T24634 T24628 cc and,ACCACAGTCCATGCCATCAC
R7061 T24635 T24636 nummod 5,TCCACCACCCTGTTGCTGTA
R7062 T24636 T24628 conj TCCACCACCCTGTTGCTGTA,ACCACAGTCCATGCCATCAC
R7063 T24637 T24635 punct ',5
R7064 T24638 T24636 punct -,TCCACCACCCTGTTGCTGTA
R7065 T24639 T24636 punct -,TCCACCACCCTGTTGCTGTA
R7066 T24640 T24636 nummod 3,TCCACCACCCTGTTGCTGTA
R7067 T24641 T24636 punct ',TCCACCACCCTGTTGCTGTA
R7068 T24642 T24643 dep which,amplified
R7069 T24643 T24628 relcl amplified,ACCACAGTCCATGCCATCAC
R7070 T24644 T24645 det a,product
R7071 T24645 T24643 dobj product,amplified
R7072 T24646 T24647 nummod 452,bp
R7073 T24647 T24645 compound bp,product
R7074 T24648 T24647 punct -,bp
R7075 T24649 T24650 punct (,587
R7076 T24650 T24643 parataxis 587,amplified
R7077 T24651 T24650 nmod positions,587
R7078 T24652 T24653 punct –,1038
R7079 T24653 T24650 prep 1038,587
R7080 T24654 T24650 prep of,587
R7081 T24655 T24656 nmod Genbank,BC85275
R7082 T24656 T24654 pobj BC85275,of
R7083 T24657 T24656 nmod Accession,BC85275
R7084 T24658 T24656 nmod Number,BC85275
R7085 T24659 T24656 punct #,BC85275
R7086 T24660 T24650 punct ),587
R7087 T24661 T24623 punct .,were