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PMC:1435744 / 42181-42722 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T24169 0-8 NNP denotes Northern
T24171 8-9 HYPH denotes -
T24170 9-13 NN denotes blot
T24172 14-22 NN denotes analysis
T24173 22-98 sentence denotes RNA was extracted from the tissues of adult mice using Trizol (Invitrogen).
T24174 23-26 NN denotes RNA
T24176 27-30 VBD denotes was
T24175 31-40 VBN denotes extracted
T24177 41-45 IN denotes from
T24178 46-49 DT denotes the
T24179 50-57 NNS denotes tissues
T24180 58-60 IN denotes of
T24181 61-66 JJ denotes adult
T24182 67-71 NNS denotes mice
T24183 72-77 VBG denotes using
T24184 78-84 NNP denotes Trizol
T24185 85-86 -LRB- denotes (
T24186 86-96 NNP denotes Invitrogen
T24187 96-97 -RRB- denotes )
T24188 97-98 . denotes .
T24189 98-235 sentence denotes A 5 μg of total RNA was electrophoresed in a 1.0% agarose-formaldehyde gel and transferred to a nylon membrane (Zeta-Probe GT, Bio-Rad).
T24190 99-100 DT denotes A
T24192 101-102 CD denotes 5
T24191 103-105 NN denotes μg
T24194 106-108 IN denotes of
T24195 109-114 JJ denotes total
T24196 115-118 NN denotes RNA
T24197 119-122 VBD denotes was
T24193 123-138 VBN denotes electrophoresed
T24198 139-141 IN denotes in
T24199 142-143 DT denotes a
T24201 144-147 CD denotes 1.0
T24202 147-148 NN denotes %
T24203 149-156 NN denotes agarose
T24205 156-157 HYPH denotes -
T24204 157-169 NN denotes formaldehyde
T24200 170-173 NN denotes gel
T24206 174-177 CC denotes and
T24207 178-189 VBN denotes transferred
T24208 190-192 IN denotes to
T24209 193-194 DT denotes a
T24211 195-200 NN denotes nylon
T24210 201-209 NN denotes membrane
T24212 210-211 -LRB- denotes (
T24214 211-215 NNP denotes Zeta
T24216 215-216 HYPH denotes -
T24215 216-221 NNP denotes Probe
T24213 222-224 NNP denotes GT
T24217 224-226 , denotes ,
T24218 226-229 NNP denotes Bio
T24220 229-230 HYPH denotes -
T24219 230-233 NNP denotes Rad
T24221 233-234 -RRB- denotes )
T24222 234-235 . denotes .
T24223 235-331 sentence denotes The 735-bp cDNA fragment encoding the SAM domain of mr-s was used as a probe for hybridization.
T24224 236-239 DT denotes The
T24226 240-243 CD denotes 735
T24228 243-244 HYPH denotes -
T24227 244-246 NN denotes bp
T24229 247-251 NN denotes cDNA
T24225 252-260 NN denotes fragment
T24231 261-269 VBG denotes encoding
T24232 270-273 DT denotes the
T24234 274-277 NN denotes SAM
T24233 278-284 NN denotes domain
T24235 285-287 IN denotes of
T24236 288-290 NN denotes mr
T24238 290-291 HYPH denotes -
T24237 291-292 NN denotes s
T24239 293-296 VBD denotes was
T24230 297-301 VBN denotes used
T24240 302-304 IN denotes as
T24241 305-306 DT denotes a
T24242 307-312 NN denotes probe
T24243 313-316 IN denotes for
T24244 317-330 NN denotes hybridization
T24245 330-331 . denotes .
T24246 331-401 sentence denotes Hybridization was performed according to the manufacturer's protocol.
T24247 332-345 NN denotes Hybridization
T24249 346-349 VBD denotes was
T24248 350-359 VBN denotes performed
T24250 360-369 VBG denotes according
T24251 370-372 IN denotes to
T24252 373-376 DT denotes the
T24253 377-389 NN denotes manufacturer
T24255 389-391 POS denotes 's
T24254 392-400 NN denotes protocol
T24256 400-401 . denotes .
T24257 401-541 sentence denotes Washes with increasing stringency were performed, the last being at 50°C in 0.1× standard saline citrate/0.1% sodium dodecyl sulfate (SDS).
T24258 402-408 NNS denotes Washes
T24260 409-413 IN denotes with
T24261 414-424 VBG denotes increasing
T24262 425-435 NN denotes stringency
T24263 436-440 VBD denotes were
T24259 441-450 VBN denotes performed
T24264 450-452 , denotes ,
T24265 452-455 DT denotes the
T24266 456-460 JJ denotes last
T24267 461-466 VBG denotes being
T24268 467-469 IN denotes at
T24269 470-472 CD denotes 50
T24270 472-474 NN denotes °C
T24271 475-477 IN denotes in
T24272 478-481 CD denotes 0.1
T24274 481-482 SYM denotes ×
T24275 483-491 JJ denotes standard
T24276 492-498 NN denotes saline
T24273 499-506 NN denotes citrate
T24277 506-507 SYM denotes /
T24279 507-510 CD denotes 0.1
T24278 510-511 NN denotes %
T24280 512-518 NN denotes sodium
T24282 519-526 NN denotes dodecyl
T24281 527-534 NN denotes sulfate
T24283 535-536 -LRB- denotes (
T24284 536-539 NN denotes SDS
T24285 539-540 -RRB- denotes )
T24286 540-541 . denotes .
R6866 T24169 T24170 compound Northern,blot
R6867 T24170 T24172 compound blot,analysis
R6868 T24171 T24170 punct -,blot
R6869 T24174 T24175 nsubjpass RNA,extracted
R6870 T24176 T24175 auxpass was,extracted
R6871 T24177 T24175 prep from,extracted
R6872 T24178 T24179 det the,tissues
R6873 T24179 T24177 pobj tissues,from
R6874 T24180 T24179 prep of,tissues
R6875 T24181 T24182 amod adult,mice
R6876 T24182 T24180 pobj mice,of
R6877 T24183 T24175 advcl using,extracted
R6878 T24184 T24183 dobj Trizol,using
R6879 T24185 T24186 punct (,Invitrogen
R6880 T24186 T24184 parataxis Invitrogen,Trizol
R6881 T24187 T24186 punct ),Invitrogen
R6882 T24188 T24175 punct .,extracted
R6883 T24190 T24191 det A,μg
R6884 T24191 T24193 nsubjpass μg,electrophoresed
R6885 T24192 T24191 nummod 5,μg
R6886 T24194 T24191 prep of,μg
R6887 T24195 T24196 amod total,RNA
R6888 T24196 T24194 pobj RNA,of
R6889 T24197 T24193 auxpass was,electrophoresed
R6890 T24198 T24193 prep in,electrophoresed
R6891 T24199 T24200 det a,gel
R6892 T24200 T24198 pobj gel,in
R6893 T24201 T24202 nummod 1.0,%
R6894 T24202 T24200 compound %,gel
R6895 T24203 T24204 compound agarose,formaldehyde
R6896 T24204 T24200 compound formaldehyde,gel
R6897 T24205 T24204 punct -,formaldehyde
R6898 T24206 T24193 cc and,electrophoresed
R6899 T24207 T24193 conj transferred,electrophoresed
R6900 T24208 T24207 prep to,transferred
R6901 T24209 T24210 det a,membrane
R6902 T24210 T24208 pobj membrane,to
R6903 T24211 T24210 compound nylon,membrane
R6904 T24212 T24213 punct (,GT
R6905 T24213 T24210 parataxis GT,membrane
R6906 T24214 T24215 compound Zeta,Probe
R6907 T24215 T24213 compound Probe,GT
R6908 T24216 T24215 punct -,Probe
R6909 T24217 T24213 punct ", ",GT
R6910 T24218 T24219 compound Bio,Rad
R6911 T24219 T24213 npadvmod Rad,GT
R6912 T24220 T24219 punct -,Rad
R6913 T24221 T24213 punct ),GT
R6914 T24222 T24193 punct .,electrophoresed
R6915 T24224 T24225 det The,fragment
R6916 T24225 T24230 nsubjpass fragment,used
R6917 T24226 T24227 nummod 735,bp
R6918 T24227 T24225 compound bp,fragment
R6919 T24228 T24227 punct -,bp
R6920 T24229 T24225 compound cDNA,fragment
R6921 T24231 T24225 acl encoding,fragment
R6922 T24232 T24233 det the,domain
R6923 T24233 T24231 dobj domain,encoding
R6924 T24234 T24233 compound SAM,domain
R6925 T24235 T24233 prep of,domain
R6926 T24236 T24237 compound mr,s
R6927 T24237 T24235 pobj s,of
R6928 T24238 T24237 punct -,s
R6929 T24239 T24230 auxpass was,used
R6930 T24240 T24230 prep as,used
R6931 T24241 T24242 det a,probe
R6932 T24242 T24240 pobj probe,as
R6933 T24243 T24230 prep for,used
R6934 T24244 T24243 pobj hybridization,for
R6935 T24245 T24230 punct .,used
R6936 T24247 T24248 nsubjpass Hybridization,performed
R6937 T24249 T24248 auxpass was,performed
R6938 T24250 T24248 prep according,performed
R6939 T24251 T24250 prep to,according
R6940 T24252 T24253 det the,manufacturer
R6941 T24253 T24254 poss manufacturer,protocol
R6942 T24254 T24251 pobj protocol,to
R6943 T24255 T24253 case 's,manufacturer
R6944 T24256 T24248 punct .,performed
R6945 T24258 T24259 nsubjpass Washes,performed
R6946 T24260 T24258 prep with,Washes
R6947 T24261 T24262 amod increasing,stringency
R6948 T24262 T24260 pobj stringency,with
R6949 T24263 T24259 auxpass were,performed
R6950 T24264 T24259 punct ", ",performed
R6951 T24265 T24266 det the,last
R6952 T24266 T24267 nsubj last,being
R6953 T24267 T24259 advcl being,performed
R6954 T24268 T24267 prep at,being
R6955 T24269 T24270 nummod 50,°C
R6956 T24270 T24268 pobj °C,at
R6957 T24271 T24267 prep in,being
R6958 T24272 T24273 nummod 0.1,citrate
R6959 T24273 T24271 pobj citrate,in
R6960 T24274 T24272 punct ×,0.1
R6961 T24275 T24273 amod standard,citrate
R6962 T24276 T24273 compound saline,citrate
R6963 T24277 T24278 punct /,%
R6964 T24278 T24273 prep %,citrate
R6965 T24279 T24278 nummod 0.1,%
R6966 T24280 T24281 compound sodium,sulfate
R6967 T24281 T24278 appos sulfate,%
R6968 T24282 T24281 compound dodecyl,sulfate
R6969 T24283 T24281 punct (,sulfate
R6970 T24284 T24281 appos SDS,sulfate
R6971 T24285 T24259 punct ),performed
R6972 T24286 T24259 punct .,performed