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PMC:1435744 / 35753-37320 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T21560 0-2 PRP denotes We
T21562 3-7 RB denotes also
T21561 8-13 VBD denotes found
T21563 14-18 IN denotes that
T21565 19-23 JJ denotes full
T21567 23-24 HYPH denotes -
T21566 24-30 NN denotes length
T21569 31-33 NN denotes mr
T21570 33-34 HYPH denotes -
T21568 34-35 NN denotes s
T21571 36-41 VBN denotes fused
T21572 42-44 IN denotes to
T21573 45-48 DT denotes the
T21575 49-53 NN denotes GAL4
T21576 54-57 NN denotes DNA
T21577 58-65 VBG denotes binding
T21574 66-72 NN denotes domain
T21578 73-74 -LRB- denotes (
T21580 74-77 NN denotes DBD
T21581 77-78 HYPH denotes -
T21579 78-81 NN denotes mrs
T21582 81-82 -RRB- denotes )
T21564 83-92 VBZ denotes functions
T21583 93-95 IN denotes as
T21584 96-97 DT denotes a
T21586 98-113 JJ denotes transcriptional
T21585 114-123 NN denotes repressor
T21587 123-124 . denotes .
T21588 124-245 sentence denotes This may support the idea that mr-s is involved in repressive complexes similar to other SAM domain-containing proteins.
T21589 125-129 DT denotes This
T21591 130-133 MD denotes may
T21590 134-141 VB denotes support
T21592 142-145 DT denotes the
T21593 146-150 NN denotes idea
T21594 151-155 IN denotes that
T21596 156-158 NN denotes mr
T21598 158-159 HYPH denotes -
T21597 159-160 NN denotes s
T21599 161-163 VBZ denotes is
T21595 164-172 VBN denotes involved
T21600 173-175 IN denotes in
T21601 176-186 JJ denotes repressive
T21602 187-196 NNS denotes complexes
T21603 197-204 JJ denotes similar
T21604 205-207 IN denotes to
T21605 208-213 JJ denotes other
T21607 214-217 NN denotes SAM
T21608 218-224 NN denotes domain
T21610 224-225 HYPH denotes -
T21609 225-235 VBG denotes containing
T21606 236-244 NN denotes proteins
T21611 244-245 . denotes .
T21612 245-393 sentence denotes Our results, however, showed that the self-association of mr-s through its SAM domain is not essential for the transcriptional repressive activity.
T21613 246-249 PRP$ denotes Our
T21614 250-257 NNS denotes results
T21616 257-259 , denotes ,
T21617 259-266 RB denotes however
T21618 266-268 , denotes ,
T21615 268-274 VBD denotes showed
T21619 275-279 IN denotes that
T21621 280-283 DT denotes the
T21623 284-288 NN denotes self
T21624 288-289 HYPH denotes -
T21622 289-300 NN denotes association
T21625 301-303 IN denotes of
T21626 304-306 NN denotes mr
T21628 306-307 HYPH denotes -
T21627 307-308 NN denotes s
T21629 309-316 IN denotes through
T21630 317-320 PRP$ denotes its
T21632 321-324 NN denotes SAM
T21631 325-331 NN denotes domain
T21620 332-334 VBZ denotes is
T21633 335-338 RB denotes not
T21634 339-348 JJ denotes essential
T21635 349-352 IN denotes for
T21636 353-356 DT denotes the
T21638 357-372 JJ denotes transcriptional
T21639 373-383 JJ denotes repressive
T21637 384-392 NN denotes activity
T21640 392-393 . denotes .
T21641 393-523 sentence denotes Polymerization of the SAM domain has been previously reported to be essential for the repressive functions of ph and TEL [43,44].
T21642 394-408 NN denotes Polymerization
T21644 409-411 IN denotes of
T21645 412-415 DT denotes the
T21647 416-419 NN denotes SAM
T21646 420-426 NN denotes domain
T21648 427-430 VBZ denotes has
T21649 431-435 VBN denotes been
T21650 436-446 RB denotes previously
T21643 447-455 VBN denotes reported
T21651 456-458 TO denotes to
T21652 459-461 VB denotes be
T21653 462-471 JJ denotes essential
T21654 472-475 IN denotes for
T21655 476-479 DT denotes the
T21657 480-490 JJ denotes repressive
T21656 491-500 NNS denotes functions
T21658 501-503 IN denotes of
T21659 504-506 NN denotes ph
T21660 507-510 CC denotes and
T21661 511-514 NN denotes TEL
T21662 515-516 -LRB- denotes [
T21664 516-518 CD denotes 43
T21665 518-519 , denotes ,
T21663 519-521 CD denotes 44
T21666 521-522 -RRB- denotes ]
T21667 522-523 . denotes .
T21668 523-814 sentence denotes On the other hand, human lethal(3) malignant brain tumor (H-L(3)MBT) protein, which also contains a SAM domain at the C-terminus, was reported as a transcriptional repressor and the repressor activity of H-L(3)MBT required mainly the presence of the MBT repeats but not the SAM domain [45].
T21669 524-526 IN denotes On
T21671 527-530 DT denotes the
T21673 531-536 JJ denotes other
T21672 537-541 NN denotes hand
T21674 541-543 , denotes ,
T21675 543-548 JJ denotes human
T21677 549-555 JJ denotes lethal
T21678 555-556 -LRB- denotes (
T21679 556-557 CD denotes 3
T21680 557-558 -RRB- denotes )
T21681 559-568 JJ denotes malignant
T21683 569-574 NN denotes brain
T21682 575-580 NN denotes tumor
T21684 581-582 -LRB- denotes (
T21686 582-583 NN denotes H
T21687 583-584 SYM denotes -
T21688 584-585 NN denotes L
T21689 585-586 -LRB- denotes (
T21685 586-587 CD denotes 3
T21690 587-588 -RRB- denotes )
T21691 588-591 NN denotes MBT
T21692 591-592 -RRB- denotes )
T21676 593-600 NN denotes protein
T21693 600-602 , denotes ,
T21694 602-607 WDT denotes which
T21696 608-612 RB denotes also
T21695 613-621 VBZ denotes contains
T21697 622-623 DT denotes a
T21699 624-627 NN denotes SAM
T21698 628-634 NN denotes domain
T21700 635-637 IN denotes at
T21701 638-641 DT denotes the
T21703 642-643 NN denotes C
T21704 643-644 HYPH denotes -
T21702 644-652 NN denotes terminus
T21705 652-654 , denotes ,
T21706 654-657 VBD denotes was
T21670 658-666 VBN denotes reported
T21707 667-669 IN denotes as
T21708 670-671 DT denotes a
T21710 672-687 JJ denotes transcriptional
T21709 688-697 NN denotes repressor
T21711 698-701 CC denotes and
T21712 702-705 DT denotes the
T21714 706-715 NN denotes repressor
T21713 716-724 NN denotes activity
T21716 725-727 IN denotes of
T21717 728-729 NN denotes H
T21719 729-730 SYM denotes -
T21720 730-731 NN denotes L
T21721 731-732 -LRB- denotes (
T21722 732-733 CD denotes 3
T21723 733-734 -RRB- denotes )
T21718 734-737 NN denotes MBT
T21715 738-746 VBD denotes required
T21724 747-753 RB denotes mainly
T21725 754-757 DT denotes the
T21726 758-766 NN denotes presence
T21727 767-769 IN denotes of
T21728 770-773 DT denotes the
T21730 774-777 NN denotes MBT
T21729 778-785 NNS denotes repeats
T21731 786-789 CC denotes but
T21732 790-793 RB denotes not
T21733 794-797 DT denotes the
T21735 798-801 NN denotes SAM
T21734 802-808 NN denotes domain
T21736 809-810 -LRB- denotes [
T21737 810-812 CD denotes 45
T21738 812-813 -RRB- denotes ]
T21739 813-814 . denotes .
T21740 814-972 sentence denotes To determine the transcriptional repressor region of the mr-s protein, we performed a luciferase assay using site-directed mutants (DBD-W404A and DBD-G453A).
T21741 815-817 TO denotes To
T21742 818-827 VB denotes determine
T21744 828-831 DT denotes the
T21746 832-847 JJ denotes transcriptional
T21747 848-857 NN denotes repressor
T21745 858-864 NN denotes region
T21748 865-867 IN denotes of
T21749 868-871 DT denotes the
T21751 872-874 NN denotes mr
T21753 874-875 HYPH denotes -
T21752 875-876 NN denotes s
T21750 877-884 NN denotes protein
T21754 884-886 , denotes ,
T21755 886-888 PRP denotes we
T21743 889-898 VBD denotes performed
T21756 899-900 DT denotes a
T21758 901-911 NN denotes luciferase
T21757 912-917 NN denotes assay
T21759 918-923 VBG denotes using
T21760 924-928 NN denotes site
T21762 928-929 HYPH denotes -
T21761 929-937 VBN denotes directed
T21763 938-945 NNS denotes mutants
T21764 946-947 -LRB- denotes (
T21766 947-950 NN denotes DBD
T21767 950-951 HYPH denotes -
T21765 951-956 NN denotes W404A
T21768 957-960 CC denotes and
T21769 961-964 NN denotes DBD
T21771 964-965 HYPH denotes -
T21770 965-970 NN denotes G453A
T21772 970-971 -RRB- denotes )
T21773 971-972 . denotes .
T21774 972-1124 sentence denotes The result showed that the reduced binding ability of self-association partially compromises the transcriptional repressive activity of mr-s (Fig. 7D).
T21775 973-976 DT denotes The
T21776 977-983 NN denotes result
T21777 984-990 VBD denotes showed
T21778 991-995 IN denotes that
T21780 996-999 DT denotes the
T21782 1000-1007 VBN denotes reduced
T21783 1008-1015 NN denotes binding
T21781 1016-1023 NN denotes ability
T21784 1024-1026 IN denotes of
T21785 1027-1031 NN denotes self
T21787 1031-1032 HYPH denotes -
T21786 1032-1043 NN denotes association
T21788 1044-1053 RB denotes partially
T21779 1054-1065 VBZ denotes compromises
T21789 1066-1069 DT denotes the
T21791 1070-1085 JJ denotes transcriptional
T21792 1086-1096 JJ denotes repressive
T21790 1097-1105 NN denotes activity
T21793 1106-1108 IN denotes of
T21794 1109-1111 NN denotes mr
T21796 1111-1112 HYPH denotes -
T21795 1112-1113 NN denotes s
T21797 1114-1115 -LRB- denotes (
T21799 1115-1119 NN denotes Fig.
T21798 1120-1122 NN denotes 7D
T21800 1122-1123 -RRB- denotes )
T21801 1123-1124 . denotes .
T21802 1124-1283 sentence denotes However, the repressive effect was more significant when DBD-tail, which does not contain SAM domain, was co-introduced with the 5xGAL4-pGL3 reporter plasmid.
T21803 1125-1132 RB denotes However
T21805 1132-1134 , denotes ,
T21806 1134-1137 DT denotes the
T21808 1138-1148 JJ denotes repressive
T21807 1149-1155 NN denotes effect
T21804 1156-1159 VBD denotes was
T21809 1160-1164 RBR denotes more
T21810 1165-1176 JJ denotes significant
T21811 1177-1181 WRB denotes when
T21813 1182-1185 NN denotes DBD
T21815 1185-1186 HYPH denotes -
T21814 1186-1190 NN denotes tail
T21816 1190-1192 , denotes ,
T21817 1192-1197 WDT denotes which
T21819 1198-1202 VBZ denotes does
T21820 1203-1206 RB denotes not
T21818 1207-1214 VB denotes contain
T21821 1215-1218 NN denotes SAM
T21822 1219-1225 NN denotes domain
T21823 1225-1227 , denotes ,
T21824 1227-1230 VBD denotes was
T21812 1231-1244 VBN denotes co-introduced
T21825 1245-1249 IN denotes with
T21826 1250-1253 DT denotes the
T21828 1254-1260 NN denotes 5xGAL4
T21830 1260-1261 HYPH denotes -
T21829 1261-1265 NN denotes pGL3
T21831 1266-1274 NN denotes reporter
T21827 1275-1282 NN denotes plasmid
T21832 1282-1283 . denotes .
T21833 1283-1418 sentence denotes Therefore, we conclude that a region from amino acids 463 to 542 is mainly responsible for the repressor activity in the case of mr-s.
T21834 1284-1293 RB denotes Therefore
T21836 1293-1295 , denotes ,
T21837 1295-1297 PRP denotes we
T21835 1298-1306 VBP denotes conclude
T21838 1307-1311 IN denotes that
T21840 1312-1313 DT denotes a
T21841 1314-1320 NN denotes region
T21842 1321-1325 IN denotes from
T21843 1326-1331 NN denotes amino
T21845 1332-1337 NNS denotes acids
T21844 1338-1341 CD denotes 463
T21846 1342-1344 IN denotes to
T21847 1345-1348 CD denotes 542
T21839 1349-1351 VBZ denotes is
T21848 1352-1358 RB denotes mainly
T21849 1359-1370 JJ denotes responsible
T21850 1371-1374 IN denotes for
T21851 1375-1378 DT denotes the
T21853 1379-1388 NN denotes repressor
T21852 1389-1397 NN denotes activity
T21854 1398-1400 IN denotes in
T21855 1401-1404 DT denotes the
T21856 1405-1409 NN denotes case
T21857 1410-1412 IN denotes of
T21858 1413-1415 NN denotes mr
T21860 1415-1416 HYPH denotes -
T21859 1416-1417 NN denotes s
T21861 1417-1418 . denotes .
T21862 1418-1567 sentence denotes Evolutionary conservation of the C-terminal 80 aa region of mr-s from zebrafish through human may underlie the functional importance of this region.
T21863 1419-1431 JJ denotes Evolutionary
T21864 1432-1444 NN denotes conservation
T21866 1445-1447 IN denotes of
T21867 1448-1451 DT denotes the
T21869 1452-1453 NN denotes C
T21871 1453-1454 HYPH denotes -
T21870 1454-1462 JJ denotes terminal
T21872 1463-1465 CD denotes 80
T21873 1466-1468 NN denotes aa
T21868 1469-1475 NN denotes region
T21874 1476-1478 IN denotes of
T21875 1479-1481 NN denotes mr
T21877 1481-1482 HYPH denotes -
T21876 1482-1483 NN denotes s
T21878 1484-1488 IN denotes from
T21879 1489-1498 NN denotes zebrafish
T21880 1499-1506 IN denotes through
T21881 1507-1512 JJ denotes human
T21882 1513-1516 MD denotes may
T21865 1517-1525 VB denotes underlie
T21883 1526-1529 DT denotes the
T21885 1530-1540 JJ denotes functional
T21884 1541-1551 NN denotes importance
T21886 1552-1554 IN denotes of
T21887 1555-1559 DT denotes this
T21888 1560-1566 NN denotes region
T21889 1566-1567 . denotes .
R5673 T21560 T21561 nsubj We,found
R5674 T21562 T21561 advmod also,found
R5676 T21563 T21564 mark that,functions
R5677 T21564 T21561 ccomp functions,found
R5678 T21565 T21566 amod full,length
R5679 T21566 T21568 compound length,s
R5681 T21567 T21566 punct -,length
R5682 T21568 T21564 nsubj s,functions
R5683 T21569 T21568 compound mr,s
R5685 T21570 T21568 punct -,s
R5686 T21571 T21568 acl fused,s
R5687 T21572 T21571 prep to,fused
R5689 T21573 T21574 det the,domain
R5690 T21574 T21572 pobj domain,to
R5691 T21575 T21574 nmod GAL4,domain
R5693 T21576 T21577 npadvmod DNA,binding
R5694 T21577 T21574 amod binding,domain
R5695 T21578 T21579 punct (,mrs
R5697 T21579 T21568 parataxis mrs,s
R5698 T21580 T21579 compound DBD,mrs
R5699 T21581 T21579 punct -,mrs
R5701 T21582 T21579 punct ),mrs
R5702 T21583 T21564 prep as,functions
R5703 T21584 T21585 det a,repressor
R5704 T21585 T21583 pobj repressor,as
R5705 T21586 T21585 amod transcriptional,repressor
R5706 T21587 T21561 punct .,found
R5707 T21595 T21593 acl involved,idea
R5708 T21589 T21590 nsubj This,support
R5709 T21591 T21590 aux may,support
R5710 T21592 T21593 det the,idea
R5711 T21596 T21597 compound mr,s
R5712 T21593 T21590 dobj idea,support
R5713 T21594 T21595 mark that,involved
R5714 T21597 T21595 nsubjpass s,involved
R5715 T21598 T21597 punct -,s
R5716 T21599 T21595 auxpass is,involved
R5717 T21600 T21595 prep in,involved
R5718 T21700 T21695 prep at,contains
R5719 T21701 T21702 det the,terminus
R5720 T21601 T21602 amod repressive,complexes
R5721 T21702 T21700 pobj terminus,at
R5722 T21703 T21702 compound C,terminus
R5723 T21602 T21600 pobj complexes,in
R5724 T21704 T21702 punct -,terminus
R5725 T21705 T21670 punct ", ",reported
R5726 T21706 T21670 auxpass was,reported
R5727 T21603 T21602 amod similar,complexes
R5728 T21707 T21670 prep as,reported
R5729 T21708 T21709 det a,repressor
R5730 T21604 T21603 prep to,similar
R5731 T21709 T21707 pobj repressor,as
R5732 T21710 T21709 amod transcriptional,repressor
R5733 T21711 T21670 cc and,reported
R5734 T21605 T21606 amod other,proteins
R5735 T21712 T21713 det the,activity
R5736 T21606 T21604 pobj proteins,to
R5737 T21713 T21715 nsubj activity,required
R5738 T21607 T21608 compound SAM,domain
R5739 T21714 T21713 compound repressor,activity
R5740 T21715 T21670 conj required,reported
R5741 T21608 T21609 npadvmod domain,containing
R5742 T21716 T21713 prep of,activity
R5743 T21717 T21718 nmod H,MBT
R5744 T21609 T21606 amod containing,proteins
R5745 T21718 T21716 pobj MBT,of
R5746 T21719 T21720 punct -,L
R5747 T21720 T21717 prep L,H
R5748 T21610 T21609 punct -,containing
R5749 T21721 T21722 punct (,3
R5750 T21722 T21717 parataxis 3,H
R5751 T21723 T21722 punct ),3
R5752 T21611 T21590 punct .,support
R5753 T21724 T21715 advmod mainly,required
R5754 T21725 T21726 det the,presence
R5755 T21726 T21715 dobj presence,required
R5756 T21727 T21726 prep of,presence
R5757 T21728 T21729 det the,repeats
R5758 T21613 T21614 poss Our,results
R5759 T21729 T21727 pobj repeats,of
R5760 T21730 T21729 compound MBT,repeats
R5761 T21731 T21729 cc but,repeats
R5762 T21732 T21731 neg not,but
R5763 T21733 T21734 det the,domain
R5764 T21614 T21615 nsubj results,showed
R5765 T21734 T21729 conj domain,repeats
R5766 T21735 T21734 compound SAM,domain
R5767 T21616 T21615 punct ", ",showed
R5768 T21736 T21737 punct [,45
R5769 T21737 T21715 parataxis 45,required
R5770 T21738 T21737 punct ],45
R5771 T21739 T21670 punct .,reported
R5772 T21617 T21615 advmod however,showed
R5773 T21741 T21742 aux To,determine
R5774 T21618 T21615 punct ", ",showed
R5775 T21742 T21743 advcl determine,performed
R5776 T21744 T21745 det the,region
R5777 T21619 T21620 mark that,is
R5778 T21745 T21742 dobj region,determine
R5779 T21746 T21745 amod transcriptional,region
R5780 T21747 T21745 compound repressor,region
R5781 T21620 T21615 ccomp is,showed
R5782 T21748 T21745 prep of,region
R5783 T21749 T21750 det the,protein
R5784 T21750 T21748 pobj protein,of
R5785 T21621 T21622 det the,association
R5786 T21751 T21752 compound mr,s
R5787 T21752 T21750 compound s,protein
R5788 T21753 T21752 punct -,s
R5789 T21622 T21620 nsubj association,is
R5790 T21754 T21743 punct ", ",performed
R5791 T21755 T21743 nsubj we,performed
R5792 T21756 T21757 det a,assay
R5793 T21623 T21622 compound self,association
R5794 T21757 T21743 dobj assay,performed
R5795 T21758 T21757 compound luciferase,assay
R5796 T21759 T21743 advcl using,performed
R5797 T21624 T21622 punct -,association
R5798 T21760 T21761 npadvmod site,directed
R5799 T21761 T21763 amod directed,mutants
R5800 T21762 T21761 punct -,directed
R5801 T21625 T21622 prep of,association
R5802 T21763 T21759 dobj mutants,using
R5803 T21764 T21765 punct (,W404A
R5804 T21765 T21743 parataxis W404A,performed
R5805 T21626 T21627 compound mr,s
R5806 T21766 T21765 compound DBD,W404A
R5807 T21767 T21765 punct -,W404A
R5808 T21768 T21765 cc and,W404A
R5809 T21769 T21770 compound DBD,G453A
R5810 T21770 T21765 conj G453A,W404A
R5811 T21771 T21770 punct -,G453A
R5812 T21772 T21765 punct ),W404A
R5813 T21627 T21625 pobj s,of
R5814 T21773 T21743 punct .,performed
R5815 T21775 T21776 det The,result
R5816 T21628 T21627 punct -,s
R5817 T21776 T21777 nsubj result,showed
R5818 T21778 T21779 mark that,compromises
R5819 T21629 T21622 prep through,association
R5820 T21779 T21777 ccomp compromises,showed
R5821 T21780 T21781 det the,ability
R5822 T21781 T21779 nsubj ability,compromises
R5823 T21630 T21631 poss its,domain
R5824 T21782 T21781 amod reduced,ability
R5825 T21783 T21781 compound binding,ability
R5826 T21631 T21629 pobj domain,through
R5827 T21784 T21781 prep of,ability
R5828 T21785 T21786 compound self,association
R5829 T21786 T21784 pobj association,of
R5830 T21632 T21631 compound SAM,domain
R5831 T21787 T21786 punct -,association
R5832 T21788 T21779 advmod partially,compromises
R5833 T21789 T21790 det the,activity
R5834 T21633 T21620 neg not,is
R5835 T21790 T21779 dobj activity,compromises
R5836 T21791 T21790 amod transcriptional,activity
R5837 T21634 T21620 acomp essential,is
R5838 T21792 T21790 amod repressive,activity
R5839 T21793 T21790 prep of,activity
R5840 T21794 T21795 compound mr,s
R5841 T21635 T21634 prep for,essential
R5842 T21795 T21793 pobj s,of
R5843 T21796 T21795 punct -,s
R5844 T21797 T21798 punct (,7D
R5845 T21636 T21637 det the,activity
R5846 T21637 T21635 pobj activity,for
R5847 T21798 T21779 parataxis 7D,compromises
R5848 T21638 T21637 amod transcriptional,activity
R5849 T21799 T21798 compound Fig.,7D
R5850 T21800 T21798 punct ),7D
R5851 T21801 T21777 punct .,showed
R5852 T21639 T21637 amod repressive,activity
R5853 T21640 T21615 punct .,showed
R5854 T21803 T21804 advmod However,was
R5855 T21642 T21643 nsubjpass Polymerization,reported
R5856 T21644 T21642 prep of,Polymerization
R5857 T21805 T21804 punct ", ",was
R5858 T21806 T21807 det the,effect
R5859 T21807 T21804 nsubj effect,was
R5860 T21808 T21807 amod repressive,effect
R5861 T21809 T21810 advmod more,significant
R5862 T21810 T21804 acomp significant,was
R5863 T21645 T21646 det the,domain
R5864 T21811 T21812 advmod when,co-introduced
R5865 T21812 T21804 advcl co-introduced,was
R5866 T21813 T21814 compound DBD,tail
R5867 T21646 T21644 pobj domain,of
R5868 T21647 T21646 compound SAM,domain
R5869 T21648 T21643 aux has,reported
R5870 T21814 T21812 nsubjpass tail,co-introduced
R5871 T21649 T21643 auxpass been,reported
R5872 T21815 T21814 punct -,tail
R5873 T21816 T21814 punct ", ",tail
R5874 T21817 T21818 dep which,contain
R5875 T21650 T21643 advmod previously,reported
R5876 T21818 T21814 relcl contain,tail
R5877 T21819 T21818 aux does,contain
R5878 T21651 T21652 aux to,be
R5879 T21820 T21818 neg not,contain
R5880 T21821 T21822 compound SAM,domain
R5881 T21822 T21818 dobj domain,contain
R5882 T21652 T21643 xcomp be,reported
R5883 T21823 T21812 punct ", ",co-introduced
R5884 T21824 T21812 auxpass was,co-introduced
R5885 T21825 T21812 prep with,co-introduced
R5886 T21653 T21652 acomp essential,be
R5887 T21826 T21827 det the,plasmid
R5888 T21827 T21825 pobj plasmid,with
R5889 T21654 T21653 prep for,essential
R5890 T21828 T21829 compound 5xGAL4,pGL3
R5891 T21829 T21827 compound pGL3,plasmid
R5892 T21830 T21829 punct -,pGL3
R5893 T21655 T21656 det the,functions
R5894 T21831 T21827 compound reporter,plasmid
R5895 T21832 T21804 punct .,was
R5896 T21656 T21654 pobj functions,for
R5897 T21834 T21835 advmod Therefore,conclude
R5898 T21657 T21656 amod repressive,functions
R5899 T21836 T21835 punct ", ",conclude
R5900 T21837 T21835 nsubj we,conclude
R5901 T21838 T21839 mark that,is
R5902 T21658 T21656 prep of,functions
R5903 T21839 T21835 ccomp is,conclude
R5904 T21840 T21841 det a,region
R5905 T21659 T21658 pobj ph,of
R5906 T21841 T21839 nsubj region,is
R5907 T21842 T21841 prep from,region
R5908 T21843 T21844 nmod amino,463
R5909 T21660 T21659 cc and,ph
R5910 T21844 T21842 pobj 463,from
R5911 T21845 T21844 nmod acids,463
R5912 T21846 T21844 prep to,463
R5913 T21847 T21846 pobj 542,to
R5914 T21848 T21849 advmod mainly,responsible
R5915 T21849 T21839 acomp responsible,is
R5916 T21661 T21659 conj TEL,ph
R5917 T21850 T21849 prep for,responsible
R5918 T21851 T21852 det the,activity
R5919 T21852 T21850 pobj activity,for
R5920 T21662 T21663 punct [,44
R5921 T21853 T21852 compound repressor,activity
R5922 T21854 T21839 prep in,is
R5923 T21855 T21856 det the,case
R5924 T21663 T21643 parataxis 44,reported
R5925 T21856 T21854 pobj case,in
R5926 T21857 T21856 prep of,case
R5927 T21664 T21663 nummod 43,44
R5928 T21858 T21859 compound mr,s
R5929 T21859 T21857 pobj s,of
R5930 T21860 T21859 punct -,s
R5931 T21665 T21663 punct ",",44
R5932 T21861 T21835 punct .,conclude
R5933 T21666 T21663 punct ],44
R5934 T21863 T21864 amod Evolutionary,conservation
R5935 T21864 T21865 nsubj conservation,underlie
R5936 T21667 T21643 punct .,reported
R5937 T21866 T21864 prep of,conservation
R5938 T21867 T21868 det the,region
R5939 T21868 T21866 pobj region,of
R5940 T21669 T21670 prep On,reported
R5941 T21869 T21870 npadvmod C,terminal
R5942 T21870 T21868 amod terminal,region
R5943 T21871 T21870 punct -,terminal
R5944 T21671 T21672 det the,hand
R5945 T21872 T21873 nummod 80,aa
R5946 T21873 T21868 compound aa,region
R5947 T21874 T21868 prep of,region
R5948 T21875 T21876 compound mr,s
R5949 T21672 T21669 pobj hand,On
R5950 T21876 T21874 pobj s,of
R5951 T21877 T21876 punct -,s
R5952 T21673 T21672 amod other,hand
R5953 T21878 T21864 prep from,conservation
R5954 T21879 T21878 pobj zebrafish,from
R5955 T21880 T21878 prep through,from
R5956 T21674 T21670 punct ", ",reported
R5957 T21881 T21880 pobj human,through
R5958 T21882 T21865 aux may,underlie
R5959 T21883 T21884 det the,importance
R5960 T21675 T21676 amod human,protein
R5961 T21884 T21865 dobj importance,underlie
R5962 T21885 T21884 amod functional,importance
R5963 T21676 T21670 nsubjpass protein,reported
R5964 T21886 T21884 prep of,importance
R5965 T21887 T21888 det this,region
R5966 T21888 T21886 pobj region,of
R5967 T21677 T21676 amod lethal,protein
R5968 T21889 T21865 punct .,underlie
R5970 T21678 T21679 punct (,3
R5973 T21679 T21677 parataxis 3,lethal
R5976 T21680 T21679 punct ),3
R5979 T21681 T21682 amod malignant,tumor
R5983 T21682 T21676 nmod tumor,protein
R5986 T21683 T21682 nmod brain,tumor
R5989 T21684 T21685 punct (,3
R5992 T21685 T21682 parataxis 3,tumor
R5994 T21686 T21685 dep H,3
R5995 T21687 T21688 punct -,L
R5996 T21688 T21686 prep L,H
R6000 T21689 T21685 punct (,3
R6003 T21690 T21685 punct ),3
R6007 T21691 T21682 appos MBT,tumor
R6009 T21692 T21676 punct ),protein
R6014 T21693 T21676 punct ", ",protein
R6021 T21694 T21695 dep which,contains
R6025 T21695 T21676 relcl contains,protein
R6029 T21696 T21695 advmod also,contains
R6033 T21697 T21698 det a,domain
R6036 T21698 T21695 dobj domain,contains
R6038 T21699 T21698 compound SAM,domain