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PMC:1435744 / 25358-31435 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T16058 0-3 DT denotes The
T16060 4-8 NN denotes GAL4
T16062 8-9 HYPH denotes -
T16063 9-11 NN denotes mr
T16065 11-12 HYPH denotes -
T16064 12-13 NN denotes s
T16061 14-20 NN denotes fusion
T16059 21-28 NN denotes protein
T16066 29-38 VBZ denotes functions
T16067 39-41 IN denotes as
T16068 42-57 JJ denotes transcriptional
T16069 58-67 NN denotes repressor
T16070 67-167 sentence denotes A member of PcG proteins, ph, does not contain an obvious sequence-specific DNA binding motif (16).
T16071 68-69 DT denotes A
T16072 70-76 NN denotes member
T16074 77-79 IN denotes of
T16075 80-83 NN denotes PcG
T16076 84-92 NN denotes proteins
T16077 92-94 , denotes ,
T16078 94-96 NN denotes ph
T16079 96-98 , denotes ,
T16080 98-102 VBZ denotes does
T16081 103-106 RB denotes not
T16073 107-114 VB denotes contain
T16082 115-117 DT denotes an
T16084 118-125 JJ denotes obvious
T16085 126-134 NN denotes sequence
T16087 134-135 HYPH denotes -
T16086 135-143 JJ denotes specific
T16088 144-147 NN denotes DNA
T16089 148-155 VBG denotes binding
T16083 156-161 NN denotes motif
T16090 162-163 -LRB- denotes (
T16091 163-165 CD denotes 16
T16092 165-166 -RRB- denotes )
T16093 166-167 . denotes .
T16094 167-378 sentence denotes Ph functions as a transcriptional repressor through its polymerization and protein-protein interaction with other sequence-specific transcriptional repressors, which can form a higher order chromatin structure.
T16095 168-170 NN denotes Ph
T16096 171-180 VBZ denotes functions
T16097 181-183 IN denotes as
T16098 184-185 DT denotes a
T16100 186-201 JJ denotes transcriptional
T16099 202-211 NN denotes repressor
T16101 212-219 IN denotes through
T16102 220-223 PRP$ denotes its
T16103 224-238 NN denotes polymerization
T16104 239-242 CC denotes and
T16105 243-250 NN denotes protein
T16107 250-251 HYPH denotes -
T16106 251-258 NN denotes protein
T16108 259-270 NN denotes interaction
T16109 271-275 IN denotes with
T16110 276-281 JJ denotes other
T16112 282-290 NN denotes sequence
T16114 290-291 HYPH denotes -
T16113 291-299 JJ denotes specific
T16115 300-315 JJ denotes transcriptional
T16111 316-326 NNS denotes repressors
T16116 326-328 , denotes ,
T16117 328-333 WDT denotes which
T16119 334-337 MD denotes can
T16118 338-342 VB denotes form
T16120 343-344 DT denotes a
T16122 345-351 JJR denotes higher
T16123 352-357 NN denotes order
T16124 358-367 NN denotes chromatin
T16121 368-377 NN denotes structure
T16125 377-378 . denotes .
T16126 378-445 sentence denotes The mr-s protein also does not have an obvious DNA-binding domain.
T16127 379-382 DT denotes The
T16129 383-385 NN denotes mr
T16131 385-386 HYPH denotes -
T16130 386-387 NN denotes s
T16128 388-395 NN denotes protein
T16133 396-400 RB denotes also
T16134 401-405 VBZ denotes does
T16135 406-409 RB denotes not
T16132 410-414 VB denotes have
T16136 415-417 DT denotes an
T16138 418-425 JJ denotes obvious
T16139 426-429 NN denotes DNA
T16141 429-430 HYPH denotes -
T16140 430-437 VBG denotes binding
T16137 438-444 NN denotes domain
T16142 444-445 . denotes .
T16143 445-533 sentence denotes To characterize the biochemical activity of mr-s, we next performed a luciferase assay.
T16144 446-448 TO denotes To
T16145 449-461 VB denotes characterize
T16147 462-465 DT denotes the
T16149 466-477 JJ denotes biochemical
T16148 478-486 NN denotes activity
T16150 487-489 IN denotes of
T16151 490-492 NN denotes mr
T16153 492-493 HYPH denotes -
T16152 493-494 NN denotes s
T16154 494-496 , denotes ,
T16155 496-498 PRP denotes we
T16156 499-503 RB denotes next
T16146 504-513 VBD denotes performed
T16157 514-515 DT denotes a
T16159 516-526 NN denotes luciferase
T16158 527-532 NN denotes assay
T16160 532-533 . denotes .
T16161 533-663 sentence denotes We generated effector plasmids, which express various deletion constructs of mr-s fused to the GAL4 DNA-binding domain (Fig. 7A).
T16162 534-536 PRP denotes We
T16163 537-546 VBD denotes generated
T16164 547-555 NN denotes effector
T16165 556-564 NNS denotes plasmids
T16166 564-566 , denotes ,
T16167 566-571 WDT denotes which
T16168 572-579 VBP denotes express
T16169 580-587 JJ denotes various
T16171 588-596 NN denotes deletion
T16170 597-607 NNS denotes constructs
T16172 608-610 IN denotes of
T16173 611-613 NN denotes mr
T16175 613-614 HYPH denotes -
T16174 614-615 NN denotes s
T16176 616-621 VBN denotes fused
T16177 622-624 IN denotes to
T16178 625-628 DT denotes the
T16180 629-633 NN denotes GAL4
T16181 634-637 NN denotes DNA
T16183 637-638 HYPH denotes -
T16182 638-645 VBG denotes binding
T16179 646-652 NN denotes domain
T16184 653-654 -LRB- denotes (
T16186 654-658 NN denotes Fig.
T16185 659-661 NN denotes 7A
T16187 661-662 -RRB- denotes )
T16188 662-663 . denotes .
T16189 663-835 sentence denotes We first confirmed that full-length mr-s fused to GAL4 DNA binding domain (DBD-mrs) had no effect on the pGL3 promoter plasmid lacking GAL4 binding sites (data not shown).
T16190 664-666 PRP denotes We
T16192 667-672 RB denotes first
T16191 673-682 VBD denotes confirmed
T16193 683-687 IN denotes that
T16195 688-692 JJ denotes full
T16197 692-693 HYPH denotes -
T16196 693-699 NN denotes length
T16199 700-702 NN denotes mr
T16200 702-703 HYPH denotes -
T16198 703-704 NN denotes s
T16201 705-710 VBN denotes fused
T16202 711-713 IN denotes to
T16203 714-718 NN denotes GAL4
T16205 719-722 NN denotes DNA
T16206 723-730 NN denotes binding
T16204 731-737 NN denotes domain
T16207 738-739 -LRB- denotes (
T16208 739-742 NN denotes DBD
T16210 742-743 HYPH denotes -
T16209 743-746 NN denotes mrs
T16211 746-747 -RRB- denotes )
T16194 748-751 VBD denotes had
T16212 752-754 DT denotes no
T16213 755-761 NN denotes effect
T16214 762-764 IN denotes on
T16215 765-768 DT denotes the
T16217 769-773 NN denotes pGL3
T16218 774-782 NN denotes promoter
T16216 783-790 NN denotes plasmid
T16219 791-798 VBG denotes lacking
T16220 799-803 NN denotes GAL4
T16221 804-811 NN denotes binding
T16222 812-817 NNS denotes sites
T16223 818-819 -LRB- denotes (
T16225 819-823 NNS denotes data
T16226 824-827 RB denotes not
T16224 828-833 VBN denotes shown
T16227 833-834 -RRB- denotes )
T16228 834-835 . denotes .
T16229 835-982 sentence denotes When the 5xGAL4-pGL3 reporter plasmid was co-transfected, DBD-mrs repressed luciferase activity by about 90% in a dose-dependent manner (Fig. 7B).
T16230 836-840 WRB denotes When
T16232 841-844 DT denotes the
T16234 845-851 NN denotes 5xGAL4
T16236 851-852 HYPH denotes -
T16235 852-856 NN denotes pGL3
T16237 857-865 NN denotes reporter
T16233 866-873 NN denotes plasmid
T16238 874-877 VBD denotes was
T16231 878-892 VBN denotes co-transfected
T16240 892-894 , denotes ,
T16241 894-897 NN denotes DBD
T16243 897-898 HYPH denotes -
T16242 898-901 NN denotes mrs
T16239 902-911 VBN denotes repressed
T16244 912-922 NN denotes luciferase
T16245 923-931 NN denotes activity
T16246 932-934 IN denotes by
T16247 935-940 IN denotes about
T16248 941-943 CD denotes 90
T16249 943-944 NN denotes %
T16250 945-947 IN denotes in
T16251 948-949 DT denotes a
T16253 950-954 NN denotes dose
T16255 954-955 HYPH denotes -
T16254 955-964 JJ denotes dependent
T16252 965-971 NN denotes manner
T16256 972-973 -LRB- denotes (
T16258 973-977 NN denotes Fig.
T16257 978-980 NN denotes 7B
T16259 980-981 -RRB- denotes )
T16260 981-982 . denotes .
T16261 982-1089 sentence denotes As a control, GAL4 DNA-binding domain (DBD) had no significant effect on the 5xGAL4-pGL3 reporter plasmid.
T16262 983-985 IN denotes As
T16264 986-987 DT denotes a
T16265 988-995 NN denotes control
T16266 995-997 , denotes ,
T16267 997-1001 NN denotes GAL4
T16269 1002-1005 NN denotes DNA
T16271 1005-1006 HYPH denotes -
T16270 1006-1013 VBG denotes binding
T16268 1014-1020 NN denotes domain
T16272 1021-1022 -LRB- denotes (
T16273 1022-1025 NN denotes DBD
T16274 1025-1026 -RRB- denotes )
T16263 1027-1030 VBD denotes had
T16275 1031-1033 DT denotes no
T16277 1034-1045 JJ denotes significant
T16276 1046-1052 NN denotes effect
T16278 1053-1055 IN denotes on
T16279 1056-1059 DT denotes the
T16281 1060-1066 NN denotes 5xGAL4
T16283 1066-1067 HYPH denotes -
T16282 1067-1071 NN denotes pGL3
T16284 1072-1080 NN denotes reporter
T16280 1081-1088 NN denotes plasmid
T16285 1088-1089 . denotes .
T16286 1089-1215 sentence denotes In addition, we confirmed that full-length mr-s without GAL4 DBD had no effect on the same reporter plasmid (data not shown).
T16287 1090-1092 IN denotes In
T16289 1093-1101 NN denotes addition
T16290 1101-1103 , denotes ,
T16291 1103-1105 PRP denotes we
T16288 1106-1115 VBD denotes confirmed
T16292 1116-1120 IN denotes that
T16294 1121-1125 JJ denotes full
T16296 1125-1126 HYPH denotes -
T16295 1126-1132 NN denotes length
T16298 1133-1135 NN denotes mr
T16299 1135-1136 HYPH denotes -
T16297 1136-1137 NN denotes s
T16300 1138-1145 IN denotes without
T16301 1146-1150 NN denotes GAL4
T16302 1151-1154 NN denotes DBD
T16293 1155-1158 VBD denotes had
T16303 1159-1161 DT denotes no
T16304 1162-1168 NN denotes effect
T16305 1169-1171 IN denotes on
T16306 1172-1175 DT denotes the
T16308 1176-1180 JJ denotes same
T16309 1181-1189 NN denotes reporter
T16307 1190-1197 NN denotes plasmid
T16310 1198-1199 -LRB- denotes (
T16312 1199-1203 NNS denotes data
T16313 1204-1207 RB denotes not
T16311 1208-1213 VBN denotes shown
T16314 1213-1214 -RRB- denotes )
T16315 1214-1215 . denotes .
T16316 1215-1425 sentence denotes We next analyzed deletion constructs in which the N-terminus 400 aa stretch of mr-s (amino acids 1 to 400) or the C-terminus portion (amino acids 391 to 542) were fused to the GAL4 DBD (Fig. 7A, DBD-N, DBD-C).
T16317 1216-1218 PRP denotes We
T16319 1219-1223 RB denotes next
T16318 1224-1232 VBD denotes analyzed
T16320 1233-1241 NN denotes deletion
T16321 1242-1252 NNS denotes constructs
T16322 1253-1255 IN denotes in
T16324 1256-1261 WDT denotes which
T16325 1262-1265 DT denotes the
T16327 1266-1267 NN denotes N
T16329 1267-1268 HYPH denotes -
T16328 1268-1276 NN denotes terminus
T16330 1277-1280 CD denotes 400
T16331 1281-1283 NN denotes aa
T16326 1284-1291 NN denotes stretch
T16332 1292-1294 IN denotes of
T16333 1295-1297 NN denotes mr
T16335 1297-1298 HYPH denotes -
T16334 1298-1299 NN denotes s
T16336 1300-1301 -LRB- denotes (
T16338 1301-1306 NN denotes amino
T16339 1307-1312 NNS denotes acids
T16337 1313-1314 CD denotes 1
T16340 1315-1317 IN denotes to
T16341 1318-1321 CD denotes 400
T16342 1321-1322 -RRB- denotes )
T16343 1323-1325 CC denotes or
T16344 1326-1329 DT denotes the
T16346 1330-1331 NN denotes C
T16348 1331-1332 HYPH denotes -
T16347 1332-1340 NN denotes terminus
T16345 1341-1348 NN denotes portion
T16349 1349-1350 -LRB- denotes (
T16351 1350-1355 NN denotes amino
T16352 1356-1361 NNS denotes acids
T16350 1362-1365 CD denotes 391
T16353 1366-1368 IN denotes to
T16354 1369-1372 CD denotes 542
T16355 1372-1373 -RRB- denotes )
T16356 1374-1378 VBD denotes were
T16323 1379-1384 VBN denotes fused
T16357 1385-1387 IN denotes to
T16358 1388-1391 DT denotes the
T16360 1392-1396 NN denotes GAL4
T16359 1397-1400 NN denotes DBD
T16361 1401-1402 -LRB- denotes (
T16363 1402-1406 NN denotes Fig.
T16362 1407-1409 NN denotes 7A
T16364 1409-1411 , denotes ,
T16365 1411-1414 NN denotes DBD
T16367 1414-1415 HYPH denotes -
T16366 1415-1416 NN denotes N
T16368 1416-1418 , denotes ,
T16369 1418-1421 NN denotes DBD
T16371 1421-1422 HYPH denotes -
T16370 1422-1423 NN denotes C
T16372 1423-1424 -RRB- denotes )
T16373 1424-1425 . denotes .
T16374 1425-1546 sentence denotes While DBD-N had no repressive effect on this reporter activity, DBD-C repressed luciferase gene expression by about 65%.
T16375 1426-1431 IN denotes While
T16377 1432-1435 NN denotes DBD
T16379 1435-1436 HYPH denotes -
T16378 1436-1437 NN denotes N
T16376 1438-1441 VBD denotes had
T16381 1442-1444 DT denotes no
T16383 1445-1455 JJ denotes repressive
T16382 1456-1462 NN denotes effect
T16384 1463-1465 IN denotes on
T16385 1466-1470 DT denotes this
T16387 1471-1479 NN denotes reporter
T16386 1480-1488 NN denotes activity
T16388 1488-1490 , denotes ,
T16389 1490-1493 NN denotes DBD
T16391 1493-1494 HYPH denotes -
T16390 1494-1495 NN denotes C
T16380 1496-1505 VBD denotes repressed
T16392 1506-1516 NN denotes luciferase
T16394 1517-1521 NN denotes gene
T16393 1522-1532 NN denotes expression
T16395 1533-1535 IN denotes by
T16396 1536-1541 IN denotes about
T16397 1542-1544 CD denotes 65
T16398 1544-1545 NN denotes %
T16399 1545-1546 . denotes .
T16400 1546-1691 sentence denotes This result suggested to us the possibility that DBD-mrs exerts transcriptional repressive activity via self-association through its SAM domain.
T16401 1547-1551 DT denotes This
T16402 1552-1558 NN denotes result
T16403 1559-1568 VBD denotes suggested
T16404 1569-1571 IN denotes to
T16405 1572-1574 PRP denotes us
T16406 1575-1578 DT denotes the
T16407 1579-1590 NN denotes possibility
T16408 1591-1595 IN denotes that
T16410 1596-1599 NN denotes DBD
T16412 1599-1600 HYPH denotes -
T16411 1600-1603 NN denotes mrs
T16409 1604-1610 VBZ denotes exerts
T16413 1611-1626 JJ denotes transcriptional
T16415 1627-1637 JJ denotes repressive
T16414 1638-1646 NN denotes activity
T16416 1647-1650 IN denotes via
T16417 1651-1655 NN denotes self
T16419 1655-1656 HYPH denotes -
T16418 1656-1667 NN denotes association
T16420 1668-1675 IN denotes through
T16421 1676-1679 PRP$ denotes its
T16423 1680-1683 NN denotes SAM
T16422 1684-1690 NN denotes domain
T16424 1690-1691 . denotes .
T16425 1691-1919 sentence denotes To investigate whether the homophilic association of mr-s is required for transcriptional repression, two site-directed mutants, DBD-W404A and DBD-G453A, either of which may reduce self-binding ability, were analyzed (Fig. 7C).
T16426 1692-1694 TO denotes To
T16427 1695-1706 VB denotes investigate
T16429 1707-1714 IN denotes whether
T16431 1715-1718 DT denotes the
T16433 1719-1729 JJ denotes homophilic
T16432 1730-1741 NN denotes association
T16434 1742-1744 IN denotes of
T16435 1745-1747 NN denotes mr
T16437 1747-1748 HYPH denotes -
T16436 1748-1749 NN denotes s
T16438 1750-1752 VBZ denotes is
T16430 1753-1761 VBN denotes required
T16439 1762-1765 IN denotes for
T16440 1766-1781 JJ denotes transcriptional
T16441 1782-1792 NN denotes repression
T16442 1792-1794 , denotes ,
T16443 1794-1797 CD denotes two
T16445 1798-1802 NN denotes site
T16447 1802-1803 HYPH denotes -
T16446 1803-1811 VBN denotes directed
T16444 1812-1819 NNS denotes mutants
T16448 1819-1821 , denotes ,
T16449 1821-1824 NN denotes DBD
T16451 1824-1825 HYPH denotes -
T16450 1825-1830 NN denotes W404A
T16452 1831-1834 CC denotes and
T16453 1835-1838 NN denotes DBD
T16455 1838-1839 HYPH denotes -
T16454 1839-1844 NN denotes G453A
T16456 1844-1846 , denotes ,
T16457 1846-1852 DT denotes either
T16459 1853-1855 IN denotes of
T16460 1856-1861 WDT denotes which
T16461 1862-1865 MD denotes may
T16458 1866-1872 VB denotes reduce
T16462 1873-1877 NN denotes self
T16464 1877-1878 HYPH denotes -
T16463 1878-1885 VBG denotes binding
T16465 1886-1893 NN denotes ability
T16466 1893-1895 , denotes ,
T16467 1895-1899 VBD denotes were
T16428 1900-1908 VBN denotes analyzed
T16468 1909-1910 -LRB- denotes (
T16470 1910-1914 NN denotes Fig.
T16469 1915-1917 NN denotes 7C
T16471 1917-1918 -RRB- denotes )
T16472 1918-1919 . denotes .
T16473 1919-2018 sentence denotes Compared to DBD-mrs, DBD-W404A and DBD-G453A had repression activity of 72% and 87%, respectively.
T16474 1920-1928 VBN denotes Compared
T16476 1929-1931 IN denotes to
T16477 1932-1935 NN denotes DBD
T16479 1935-1936 HYPH denotes -
T16478 1936-1939 NN denotes mrs
T16480 1939-1941 , denotes ,
T16481 1941-1944 NN denotes DBD
T16483 1944-1945 HYPH denotes -
T16482 1945-1950 NN denotes W404A
T16484 1951-1954 CC denotes and
T16485 1955-1958 NN denotes DBD
T16487 1958-1959 HYPH denotes -
T16486 1959-1964 NN denotes G453A
T16475 1965-1968 VBD denotes had
T16488 1969-1979 NN denotes repression
T16489 1980-1988 NN denotes activity
T16490 1989-1991 IN denotes of
T16491 1992-1994 CD denotes 72
T16492 1994-1995 NN denotes %
T16493 1996-1999 CC denotes and
T16494 2000-2002 CD denotes 87
T16495 2002-2003 NN denotes %
T16496 2003-2005 , denotes ,
T16497 2005-2017 RB denotes respectively
T16498 2017-2018 . denotes .
T16499 2018-2205 sentence denotes While the ability of mr-s self-association partially correlates with transcriptional repressive activity, these mutants do not compromise the ability to repress transcription critically.
T16500 2019-2024 IN denotes While
T16502 2025-2028 DT denotes the
T16503 2029-2036 NN denotes ability
T16504 2037-2039 IN denotes of
T16505 2040-2042 NN denotes mr
T16507 2042-2043 HYPH denotes -
T16506 2043-2044 NN denotes s
T16509 2045-2049 NN denotes self
T16510 2049-2050 HYPH denotes -
T16508 2050-2061 NN denotes association
T16511 2062-2071 RB denotes partially
T16501 2072-2082 VBZ denotes correlates
T16513 2083-2087 IN denotes with
T16514 2088-2103 JJ denotes transcriptional
T16516 2104-2114 JJ denotes repressive
T16515 2115-2123 NN denotes activity
T16517 2123-2125 , denotes ,
T16518 2125-2130 DT denotes these
T16519 2131-2138 NNS denotes mutants
T16520 2139-2141 VBP denotes do
T16521 2142-2145 RB denotes not
T16512 2146-2156 VB denotes compromise
T16522 2157-2160 DT denotes the
T16523 2161-2168 NN denotes ability
T16524 2169-2171 TO denotes to
T16525 2172-2179 VB denotes repress
T16526 2180-2193 NN denotes transcription
T16527 2194-2204 RB denotes critically
T16528 2204-2205 . denotes .
T16529 2205-2410 sentence denotes To determine the regions of mr-s involved in transcriptional repression more precisely, the C-terminus portion was divided into two regions and each was fused to the GAL4 DBD (Fig. 7A, DBD-tail, DBD-SAM).
T16530 2206-2208 TO denotes To
T16531 2209-2218 VB denotes determine
T16533 2219-2222 DT denotes the
T16534 2223-2230 NNS denotes regions
T16535 2231-2233 IN denotes of
T16536 2234-2236 NN denotes mr
T16538 2236-2237 HYPH denotes -
T16537 2237-2238 NN denotes s
T16539 2239-2247 VBN denotes involved
T16540 2248-2250 IN denotes in
T16541 2251-2266 JJ denotes transcriptional
T16542 2267-2277 NN denotes repression
T16543 2278-2282 RBR denotes more
T16544 2283-2292 RB denotes precisely
T16545 2292-2294 , denotes ,
T16546 2294-2297 DT denotes the
T16548 2298-2299 NN denotes C
T16550 2299-2300 HYPH denotes -
T16549 2300-2308 NN denotes terminus
T16547 2309-2316 NN denotes portion
T16551 2317-2320 VBD denotes was
T16532 2321-2328 VBN denotes divided
T16552 2329-2333 IN denotes into
T16553 2334-2337 CD denotes two
T16554 2338-2345 NNS denotes regions
T16555 2346-2349 CC denotes and
T16556 2350-2354 DT denotes each
T16558 2355-2358 VBD denotes was
T16557 2359-2364 VBN denotes fused
T16559 2365-2367 IN denotes to
T16560 2368-2371 DT denotes the
T16562 2372-2376 NN denotes GAL4
T16561 2377-2380 NN denotes DBD
T16563 2381-2382 -LRB- denotes (
T16565 2382-2386 NN denotes Fig.
T16566 2387-2389 NN denotes 7A
T16567 2389-2391 , denotes ,
T16568 2391-2394 NN denotes DBD
T16570 2394-2395 HYPH denotes -
T16569 2395-2399 NN denotes tail
T16571 2399-2401 , denotes ,
T16572 2401-2404 NN denotes DBD
T16573 2404-2405 HYPH denotes -
T16564 2405-2408 NN denotes SAM
T16574 2408-2409 -RRB- denotes )
T16575 2409-2410 . denotes .
T16576 2410-2531 sentence denotes As a consequence, DBD-SAM (amino acids 384 to 462) did not have a repressive effect on the 5xGAL4-pGL3 reporter plasmid.
T16577 2411-2413 IN denotes As
T16579 2414-2415 DT denotes a
T16580 2416-2427 NN denotes consequence
T16581 2427-2429 , denotes ,
T16582 2429-2432 NN denotes DBD
T16584 2432-2433 HYPH denotes -
T16583 2433-2436 NN denotes SAM
T16585 2437-2438 -LRB- denotes (
T16587 2438-2443 NN denotes amino
T16588 2444-2449 NNS denotes acids
T16586 2450-2453 CD denotes 384
T16589 2454-2456 IN denotes to
T16590 2457-2460 CD denotes 462
T16591 2460-2461 -RRB- denotes )
T16592 2462-2465 VBD denotes did
T16593 2466-2469 RB denotes not
T16578 2470-2474 VB denotes have
T16594 2475-2476 DT denotes a
T16596 2477-2487 JJ denotes repressive
T16595 2488-2494 NN denotes effect
T16597 2495-2497 IN denotes on
T16598 2498-2501 DT denotes the
T16600 2502-2508 NN denotes 5xGAL4
T16602 2508-2509 HYPH denotes -
T16601 2509-2513 NN denotes pGL3
T16603 2514-2522 NN denotes reporter
T16599 2523-2530 NN denotes plasmid
T16604 2530-2531 . denotes .
T16605 2531-2687 sentence denotes On the other hand, luciferase activity was repressed by 55% when DBD-tail (amino acids 459 to 542) was co-transfected with this reporter plasmid (Fig. 7D).
T16606 2532-2534 IN denotes On
T16608 2535-2538 DT denotes the
T16610 2539-2544 JJ denotes other
T16609 2545-2549 NN denotes hand
T16611 2549-2551 , denotes ,
T16612 2551-2561 NN denotes luciferase
T16613 2562-2570 NN denotes activity
T16614 2571-2574 VBD denotes was
T16607 2575-2584 VBN denotes repressed
T16615 2585-2587 IN denotes by
T16616 2588-2590 CD denotes 55
T16617 2590-2591 NN denotes %
T16618 2592-2596 WRB denotes when
T16620 2597-2600 NN denotes DBD
T16622 2600-2601 HYPH denotes -
T16621 2601-2605 NN denotes tail
T16623 2606-2607 -LRB- denotes (
T16625 2607-2612 NN denotes amino
T16626 2613-2618 NNS denotes acids
T16624 2619-2622 CD denotes 459
T16627 2623-2625 IN denotes to
T16628 2626-2629 CD denotes 542
T16629 2629-2630 -RRB- denotes )
T16630 2631-2634 VBD denotes was
T16619 2635-2649 VBN denotes co-transfected
T16631 2650-2654 IN denotes with
T16632 2655-2659 DT denotes this
T16634 2660-2668 NN denotes reporter
T16633 2669-2676 NN denotes plasmid
T16635 2677-2678 -LRB- denotes (
T16637 2678-2682 NN denotes Fig.
T16636 2683-2685 NN denotes 7D
T16638 2685-2686 -RRB- denotes )
T16639 2686-2687 . denotes .
T16640 2687-2844 sentence denotes To assess the transcriptional repressive activity of mr-s in cells of retinal origin, we performed similar experiments using human Y79 retinoblastoma cells.
T16641 2688-2690 TO denotes To
T16642 2691-2697 VB denotes assess
T16644 2698-2701 DT denotes the
T16646 2702-2717 JJ denotes transcriptional
T16647 2718-2728 JJ denotes repressive
T16645 2729-2737 NN denotes activity
T16648 2738-2740 IN denotes of
T16649 2741-2743 NN denotes mr
T16651 2743-2744 HYPH denotes -
T16650 2744-2745 NN denotes s
T16652 2746-2748 IN denotes in
T16653 2749-2754 NNS denotes cells
T16654 2755-2757 IN denotes of
T16655 2758-2765 JJ denotes retinal
T16656 2766-2772 NN denotes origin
T16657 2772-2774 , denotes ,
T16658 2774-2776 PRP denotes we
T16643 2777-2786 VBD denotes performed
T16659 2787-2794 JJ denotes similar
T16660 2795-2806 NNS denotes experiments
T16661 2807-2812 VBG denotes using
T16662 2813-2818 JJ denotes human
T16664 2819-2822 NN denotes Y79
T16665 2823-2837 NN denotes retinoblastoma
T16663 2838-2843 NNS denotes cells
T16666 2843-2844 . denotes .
T16667 2844-2965 sentence denotes The results indicated that DBD-mrs also reduced luciferase activity significantly in Y79 retinoblastoma cells (Fig. 7E).
T16668 2845-2848 DT denotes The
T16669 2849-2856 NNS denotes results
T16670 2857-2866 VBD denotes indicated
T16671 2867-2871 IN denotes that
T16673 2872-2875 NN denotes DBD
T16675 2875-2876 HYPH denotes -
T16674 2876-2879 NN denotes mrs
T16676 2880-2884 RB denotes also
T16672 2885-2892 VBD denotes reduced
T16677 2893-2903 NN denotes luciferase
T16678 2904-2912 NN denotes activity
T16679 2913-2926 RB denotes significantly
T16680 2927-2929 IN denotes in
T16681 2930-2933 NN denotes Y79
T16683 2934-2948 NN denotes retinoblastoma
T16682 2949-2954 NNS denotes cells
T16684 2955-2956 -LRB- denotes (
T16686 2956-2960 NN denotes Fig.
T16685 2961-2963 NN denotes 7E
T16687 2963-2964 -RRB- denotes )
T16688 2964-2965 . denotes .
T16689 2965-3083 sentence denotes However, luciferase activity was repressed by about 30% in Y79 cells, while it was repressed by 90% in HEK293T cells.
T16690 2966-2973 RB denotes However
T16692 2973-2975 , denotes ,
T16693 2975-2985 NN denotes luciferase
T16694 2986-2994 NN denotes activity
T16695 2995-2998 VBD denotes was
T16691 2999-3008 VBN denotes repressed
T16696 3009-3011 IN denotes by
T16697 3012-3017 IN denotes about
T16698 3018-3020 CD denotes 30
T16699 3020-3021 NN denotes %
T16700 3022-3024 IN denotes in
T16701 3025-3028 NN denotes Y79
T16702 3029-3034 NNS denotes cells
T16703 3034-3036 , denotes ,
T16704 3036-3041 IN denotes while
T16706 3042-3044 PRP denotes it
T16707 3045-3048 VBD denotes was
T16705 3049-3058 VBN denotes repressed
T16708 3059-3061 IN denotes by
T16709 3062-3064 CD denotes 90
T16710 3064-3065 NN denotes %
T16711 3066-3068 IN denotes in
T16712 3069-3076 NN denotes HEK293T
T16713 3077-3082 NNS denotes cells
T16714 3082-3083 . denotes .
T16715 3083-3172 sentence denotes This might be due to the difference in transfection efficiency between these cell lines.
T16716 3084-3088 DT denotes This
T16718 3089-3094 MD denotes might
T16717 3095-3097 VB denotes be
T16719 3098-3101 IN denotes due
T16720 3102-3104 IN denotes to
T16721 3105-3108 DT denotes the
T16722 3109-3119 NN denotes difference
T16723 3120-3122 IN denotes in
T16724 3123-3135 NN denotes transfection
T16725 3136-3146 NN denotes efficiency
T16726 3147-3154 IN denotes between
T16727 3155-3160 DT denotes these
T16729 3161-3165 NN denotes cell
T16728 3166-3171 NNS denotes lines
T16730 3171-3172 . denotes .
T16731 3172-3330 sentence denotes Another possibility is that intracellular environment in Y79 cells, a retinoblastoma cell line, is insufficient for recapitulating developing photoreceptors.
T16732 3173-3180 DT denotes Another
T16733 3181-3192 NN denotes possibility
T16734 3193-3195 VBZ denotes is
T16735 3196-3200 IN denotes that
T16737 3201-3214 JJ denotes intracellular
T16738 3215-3226 NN denotes environment
T16739 3227-3229 IN denotes in
T16740 3230-3233 NN denotes Y79
T16741 3234-3239 NNS denotes cells
T16742 3239-3241 , denotes ,
T16743 3241-3242 DT denotes a
T16745 3243-3257 NN denotes retinoblastoma
T16746 3258-3262 NN denotes cell
T16744 3263-3267 NN denotes line
T16747 3267-3269 , denotes ,
T16736 3269-3271 VBZ denotes is
T16748 3272-3284 JJ denotes insufficient
T16749 3285-3288 IN denotes for
T16750 3289-3303 VBG denotes recapitulating
T16751 3304-3314 VBG denotes developing
T16752 3315-3329 NNS denotes photoreceptors
T16753 3329-3330 . denotes .
T16754 3330-3466 sentence denotes In this study, we did not address the question whether or not our in vitro data reflect native mr-s transcriptional repression in vivo.
T16755 3331-3333 IN denotes In
T16757 3334-3338 DT denotes this
T16758 3339-3344 NN denotes study
T16759 3344-3346 , denotes ,
T16760 3346-3348 PRP denotes we
T16761 3349-3352 VBD denotes did
T16762 3353-3356 RB denotes not
T16756 3357-3364 VB denotes address
T16763 3365-3368 DT denotes the
T16764 3369-3377 NN denotes question
T16765 3378-3385 IN denotes whether
T16767 3386-3388 CC denotes or
T16768 3389-3392 RB denotes not
T16769 3393-3396 PRP$ denotes our
T16771 3397-3399 FW denotes in
T16772 3400-3405 FW denotes vitro
T16770 3406-3410 NNS denotes data
T16766 3411-3418 VBP denotes reflect
T16773 3419-3425 JJ denotes native
T16775 3426-3428 NN denotes mr
T16777 3428-3429 HYPH denotes -
T16776 3429-3430 NN denotes s
T16778 3431-3446 JJ denotes transcriptional
T16774 3447-3457 NN denotes repression
T16779 3458-3460 FW denotes in
T16780 3461-3465 FW denotes vivo
T16781 3465-3466 . denotes .
T16782 3466-3643 sentence denotes However, these in vitro experiments using HEK293T and Y79 cells strongly support our hypothesis that mr-s functions as a transcriptional repressor in developing photoreceptors.
T16783 3467-3474 RB denotes However
T16785 3474-3476 , denotes ,
T16786 3476-3481 DT denotes these
T16788 3482-3484 FW denotes in
T16789 3485-3490 FW denotes vitro
T16787 3491-3502 NNS denotes experiments
T16790 3503-3508 VBG denotes using
T16791 3509-3516 NN denotes HEK293T
T16793 3517-3520 CC denotes and
T16794 3521-3524 NN denotes Y79
T16792 3525-3530 NNS denotes cells
T16795 3531-3539 RB denotes strongly
T16784 3540-3547 VBP denotes support
T16796 3548-3551 PRP$ denotes our
T16797 3552-3562 NN denotes hypothesis
T16798 3563-3567 IN denotes that
T16800 3568-3570 NN denotes mr
T16802 3570-3571 HYPH denotes -
T16801 3571-3572 NN denotes s
T16799 3573-3582 VBZ denotes functions
T16803 3583-3585 IN denotes as
T16804 3586-3587 DT denotes a
T16806 3588-3603 JJ denotes transcriptional
T16805 3604-3613 NN denotes repressor
T16807 3614-3616 IN denotes in
T16808 3617-3627 VBG denotes developing
T16809 3628-3642 NNS denotes photoreceptors
T16810 3642-3643 . denotes .
T16811 3643-3851 sentence denotes Our results suggested that the C-terminal region of mr-s (amino acids 463 to 542) is required for transcriptional repression of mr-s and the SAM domain appears to be dispensable for this repressive activity.
T16812 3644-3647 PRP$ denotes Our
T16813 3648-3655 NNS denotes results
T16814 3656-3665 VBD denotes suggested
T16815 3666-3670 IN denotes that
T16817 3671-3674 DT denotes the
T16819 3675-3676 NN denotes C
T16821 3676-3677 HYPH denotes -
T16820 3677-3685 JJ denotes terminal
T16818 3686-3692 NN denotes region
T16822 3693-3695 IN denotes of
T16823 3696-3698 NN denotes mr
T16825 3698-3699 HYPH denotes -
T16824 3699-3700 NN denotes s
T16826 3701-3702 -LRB- denotes (
T16828 3702-3707 NN denotes amino
T16829 3708-3713 NNS denotes acids
T16827 3714-3717 CD denotes 463
T16830 3718-3720 IN denotes to
T16831 3721-3724 CD denotes 542
T16832 3724-3725 -RRB- denotes )
T16833 3726-3728 VBZ denotes is
T16816 3729-3737 VBN denotes required
T16834 3738-3741 IN denotes for
T16835 3742-3757 JJ denotes transcriptional
T16836 3758-3768 NN denotes repression
T16837 3769-3771 IN denotes of
T16838 3772-3774 NN denotes mr
T16840 3774-3775 HYPH denotes -
T16839 3775-3776 NN denotes s
T16841 3777-3780 CC denotes and
T16842 3781-3784 DT denotes the
T16844 3785-3788 NN denotes SAM
T16843 3789-3795 NN denotes domain
T16845 3796-3803 VBZ denotes appears
T16846 3804-3806 TO denotes to
T16847 3807-3809 VB denotes be
T16848 3810-3821 JJ denotes dispensable
T16849 3822-3825 IN denotes for
T16850 3826-3830 DT denotes this
T16852 3831-3841 JJ denotes repressive
T16851 3842-3850 NN denotes activity
T16853 3850-3851 . denotes .
T16854 3851-3933 sentence denotes This C-terminal region of mouse mr-s is highly conserved among species (Fig. 7F).
T16855 3852-3856 DT denotes This
T16857 3857-3858 NN denotes C
T16859 3858-3859 HYPH denotes -
T16858 3859-3867 JJ denotes terminal
T16856 3868-3874 NN denotes region
T16861 3875-3877 IN denotes of
T16862 3878-3883 NN denotes mouse
T16864 3884-3886 NN denotes mr
T16865 3886-3887 HYPH denotes -
T16863 3887-3888 NN denotes s
T16866 3889-3891 VBZ denotes is
T16867 3892-3898 RB denotes highly
T16860 3899-3908 VBN denotes conserved
T16868 3909-3914 IN denotes among
T16869 3915-3922 NNS denotes species
T16870 3923-3924 -LRB- denotes (
T16872 3924-3928 NN denotes Fig.
T16871 3929-3931 NN denotes 7F
T16873 3931-3932 -RRB- denotes )
T16874 3932-3933 . denotes .
T16875 3933-4056 sentence denotes The sequence identity of the region was 93%, 73%, 41% and 40% for rat, human, chick and zebrafish, respectively (Fig. 1D).
T16876 3934-3937 DT denotes The
T16878 3938-3946 NN denotes sequence
T16877 3947-3955 NN denotes identity
T16880 3956-3958 IN denotes of
T16881 3959-3962 DT denotes the
T16882 3963-3969 NN denotes region
T16879 3970-3973 VBD denotes was
T16883 3974-3976 CD denotes 93
T16884 3976-3977 NN denotes %
T16885 3977-3979 , denotes ,
T16886 3979-3981 CD denotes 73
T16887 3981-3982 NN denotes %
T16888 3982-3984 , denotes ,
T16889 3984-3986 CD denotes 41
T16890 3986-3987 NN denotes %
T16891 3988-3991 CC denotes and
T16892 3992-3994 CD denotes 40
T16893 3994-3995 NN denotes %
T16894 3996-3999 IN denotes for
T16895 4000-4003 NN denotes rat
T16896 4003-4005 , denotes ,
T16897 4005-4010 JJ denotes human
T16898 4010-4012 , denotes ,
T16899 4012-4017 NN denotes chick
T16900 4018-4021 CC denotes and
T16901 4022-4031 NN denotes zebrafish
T16902 4031-4033 , denotes ,
T16903 4033-4045 RB denotes respectively
T16904 4046-4047 -LRB- denotes (
T16906 4047-4051 NN denotes Fig.
T16905 4052-4054 NN denotes 1D
T16907 4054-4055 -RRB- denotes )
T16908 4055-4056 . denotes .
T16909 4056-4199 sentence denotes This strongly suggests that the C-terminal region of mouse mr-s functions as a transcriptional repressive domain in photoreceptor development.
T16910 4057-4061 DT denotes This
T16912 4062-4070 RB denotes strongly
T16911 4071-4079 VBZ denotes suggests
T16913 4080-4084 IN denotes that
T16915 4085-4088 DT denotes the
T16917 4089-4090 NN denotes C
T16919 4090-4091 HYPH denotes -
T16918 4091-4099 JJ denotes terminal
T16916 4100-4106 NN denotes region
T16920 4107-4109 IN denotes of
T16921 4110-4115 NN denotes mouse
T16923 4116-4118 NN denotes mr
T16924 4118-4119 HYPH denotes -
T16922 4119-4120 NN denotes s
T16914 4121-4130 VBZ denotes functions
T16925 4131-4133 IN denotes as
T16926 4134-4135 DT denotes a
T16928 4136-4151 JJ denotes transcriptional
T16929 4152-4162 JJ denotes repressive
T16927 4163-4169 NN denotes domain
T16930 4170-4172 IN denotes in
T16931 4173-4186 NN denotes photoreceptor
T16932 4187-4198 NN denotes development
T16933 4198-4199 . denotes .
T16934 4199-4394 sentence denotes However, this region does not contain characteristic amino acid motifs and the mechanism through which the region achieves and/or maintains gene repression remains to be clarified in the future.
T16935 4200-4207 RB denotes However
T16937 4207-4209 , denotes ,
T16938 4209-4213 DT denotes this
T16939 4214-4220 NN denotes region
T16940 4221-4225 VBZ denotes does
T16941 4226-4229 RB denotes not
T16936 4230-4237 VB denotes contain
T16942 4238-4252 JJ denotes characteristic
T16943 4253-4258 NN denotes amino
T16944 4259-4263 NN denotes acid
T16945 4264-4270 NNS denotes motifs
T16946 4271-4274 CC denotes and
T16947 4275-4278 DT denotes the
T16948 4279-4288 NN denotes mechanism
T16950 4289-4296 IN denotes through
T16952 4297-4302 WDT denotes which
T16953 4303-4306 DT denotes the
T16954 4307-4313 NN denotes region
T16951 4314-4322 VBZ denotes achieves
T16955 4323-4326 CC denotes and
T16956 4326-4327 HYPH denotes /
T16957 4327-4329 CC denotes or
T16958 4330-4339 VBZ denotes maintains
T16959 4340-4344 NN denotes gene
T16960 4345-4355 NN denotes repression
T16949 4356-4363 VBZ denotes remains
T16961 4364-4366 TO denotes to
T16963 4367-4369 VB denotes be
T16962 4370-4379 VBN denotes clarified
T16964 4380-4382 IN denotes in
T16965 4383-4386 DT denotes the
T16966 4387-4393 NN denotes future
T16967 4393-4394 . denotes .
T16968 4394-6077 sentence denotes Figure 7 mr-s fused to GAL4 DNA binding domain functions as a transcriptional repressor in HEK293T cells. (A) Schematic drawing of the constructs used for the luciferase assay. 5xGAL4-pGL3 reporter plasmid was co-transfected into HEK293T cells with effector plasmids expressing various deletion mutants fused to GAL4-DBD. (B) Various amounts of DBD, DBD-mrs, DBD-N or DBD-C plasmids were transfected with 0.1 μg of 5xGAL4-pGL3 reporter plasmid. The reporter activity in the presence of the pcDNA3 vector (pcDNA3) was designated as 1. Error bars represent standard error of mean. (C) DBD-W404A and DBD-G453A were co-transfected into HEK293T cells with 5xGAL4-pGL3 reporter plasmid. Fold repression was calculated as the fold decrease in luciferase activity compared with DBD-mrs. Error bars represent standard deviation. (D) Various amounts of DBD-tail or DBD-SAM were transfected with 5xGAL4-pGL3 reporter plasmid. Error bars represent standard error of mean. (E) pcDNA3 or DBD-mrs (5 μg) was co-transfected into Y79 retinoblastoma cells with 0.5 μg of 5xGAL4-pGL3 reporter plasmid. The reporter activity in the presence of pcDNA3 was designated as 1. Error bars represent standard deviation. Asterisk marks statistically significant difference (Student's t test: p < 0.03). (F) Alignment of the C-terminal regions for mouse, rat, human, chick and zebrafish mr-s proteins. Conserved amino acid residues are shown with a dark shadow. Taken together, our findings suggest that DBD-mrs functions as a transcriptional repressor and that the repression activity of mr-s is not due to a homophilic interaction through its SAM domain but to the C-terminal region (amino acids 463 to 542).
T32444 4405-4407 NN denotes mr
T32446 4407-4408 HYPH denotes -
T32445 4408-4409 NN denotes s
T32448 4410-4415 VBN denotes fused
T32449 4416-4418 IN denotes to
T32450 4419-4423 NN denotes GAL4
T32452 4424-4427 NN denotes DNA
T32453 4428-4435 VBG denotes binding
T32451 4436-4442 NN denotes domain
T32447 4443-4452 VBZ denotes functions
T32454 4453-4455 IN denotes as
T32455 4456-4457 DT denotes a
T32457 4458-4473 JJ denotes transcriptional
T32456 4474-4483 NN denotes repressor
T32458 4484-4486 IN denotes in
T32459 4487-4494 NN denotes HEK293T
T32460 4495-4500 NNS denotes cells
T32461 4500-4501 . denotes .
T32462 4501-4572 sentence denotes (A) Schematic drawing of the constructs used for the luciferase assay.
T32463 4502-4503 -LRB- denotes (
T32464 4503-4504 LS denotes A
T32466 4504-4505 -RRB- denotes )
T32467 4506-4515 JJ denotes Schematic
T32465 4516-4523 NN denotes drawing
T32468 4524-4526 IN denotes of
T32469 4527-4530 DT denotes the
T32470 4531-4541 NNS denotes constructs
T32471 4542-4546 VBN denotes used
T32472 4547-4550 IN denotes for
T32473 4551-4554 DT denotes the
T32475 4555-4565 NN denotes luciferase
T32474 4566-4571 NN denotes assay
T32476 4571-4572 . denotes .
T32477 4572-4717 sentence denotes 5xGAL4-pGL3 reporter plasmid was co-transfected into HEK293T cells with effector plasmids expressing various deletion mutants fused to GAL4-DBD.
T32478 4573-4579 NN denotes 5xGAL4
T32480 4579-4580 HYPH denotes -
T32479 4580-4584 NN denotes pGL3
T32482 4585-4593 NN denotes reporter
T32481 4594-4601 NN denotes plasmid
T32484 4602-4605 VBD denotes was
T32483 4606-4620 VBN denotes co-transfected
T32485 4621-4625 IN denotes into
T32486 4626-4633 NN denotes HEK293T
T32487 4634-4639 NNS denotes cells
T32488 4640-4644 IN denotes with
T32489 4645-4653 NN denotes effector
T32490 4654-4662 NNS denotes plasmids
T32491 4663-4673 VBG denotes expressing
T32492 4674-4681 JJ denotes various
T32494 4682-4690 NN denotes deletion
T32493 4691-4698 NNS denotes mutants
T32495 4699-4704 VBN denotes fused
T32496 4705-4707 IN denotes to
T32497 4708-4712 NN denotes GAL4
T32499 4712-4713 HYPH denotes -
T32498 4713-4716 NN denotes DBD
T32500 4716-4717 . denotes .
T32501 4717-4840 sentence denotes (B) Various amounts of DBD, DBD-mrs, DBD-N or DBD-C plasmids were transfected with 0.1 μg of 5xGAL4-pGL3 reporter plasmid.
T32502 4718-4719 -LRB- denotes (
T32503 4719-4720 LS denotes B
T32505 4720-4721 -RRB- denotes )
T32506 4722-4729 JJ denotes Various
T32507 4730-4737 NNS denotes amounts
T32508 4738-4740 IN denotes of
T32509 4741-4744 NN denotes DBD
T32511 4744-4746 , denotes ,
T32512 4746-4749 NN denotes DBD
T32514 4749-4750 HYPH denotes -
T32513 4750-4753 NN denotes mrs
T32515 4753-4755 , denotes ,
T32516 4755-4758 NN denotes DBD
T32518 4758-4759 HYPH denotes -
T32517 4759-4760 NN denotes N
T32519 4761-4763 CC denotes or
T32520 4764-4767 NN denotes DBD
T32522 4767-4768 HYPH denotes -
T32521 4768-4769 NN denotes C
T32510 4770-4778 NNS denotes plasmids
T32523 4779-4783 VBD denotes were
T32504 4784-4795 VBN denotes transfected
T32524 4796-4800 IN denotes with
T32525 4801-4804 CD denotes 0.1
T32526 4805-4807 NN denotes μg
T32527 4808-4810 IN denotes of
T32528 4811-4817 NN denotes 5xGAL4
T32530 4817-4818 HYPH denotes -
T32529 4818-4822 NN denotes pGL3
T32532 4823-4831 NN denotes reporter
T32531 4832-4839 NN denotes plasmid
T32533 4839-4840 . denotes .
T32534 4840-4929 sentence denotes The reporter activity in the presence of the pcDNA3 vector (pcDNA3) was designated as 1.
T32535 4841-4844 DT denotes The
T32537 4845-4853 NN denotes reporter
T32536 4854-4862 NN denotes activity
T32539 4863-4865 IN denotes in
T32540 4866-4869 DT denotes the
T32541 4870-4878 NN denotes presence
T32542 4879-4881 IN denotes of
T32543 4882-4885 DT denotes the
T32545 4886-4892 NN denotes pcDNA3
T32544 4893-4899 NN denotes vector
T32546 4900-4901 -LRB- denotes (
T32547 4901-4907 NN denotes pcDNA3
T32548 4907-4908 -RRB- denotes )
T32549 4909-4912 VBD denotes was
T32538 4913-4923 VBN denotes designated
T32550 4924-4926 IN denotes as
T32551 4927-4928 CD denotes 1
T32552 4928-4929 . denotes .
T32553 4929-4974 sentence denotes Error bars represent standard error of mean.
T32554 4930-4935 NN denotes Error
T32555 4936-4940 NNS denotes bars
T32556 4941-4950 VBP denotes represent
T32557 4951-4959 JJ denotes standard
T32558 4960-4965 NN denotes error
T32559 4966-4968 IN denotes of
T32560 4969-4973 NN denotes mean
T32561 4973-4974 . denotes .
T32562 4974-5076 sentence denotes (C) DBD-W404A and DBD-G453A were co-transfected into HEK293T cells with 5xGAL4-pGL3 reporter plasmid.
T32563 4975-4976 -LRB- denotes (
T32564 4976-4977 LS denotes C
T32566 4977-4978 -RRB- denotes )
T32567 4979-4982 NN denotes DBD
T32569 4982-4983 HYPH denotes -
T32568 4983-4988 NN denotes W404A
T32570 4989-4992 CC denotes and
T32571 4993-4996 NN denotes DBD
T32573 4996-4997 HYPH denotes -
T32572 4997-5002 NN denotes G453A
T32574 5003-5007 VBD denotes were
T32565 5008-5022 VBN denotes co-transfected
T32575 5023-5027 IN denotes into
T32576 5028-5035 NN denotes HEK293T
T32577 5036-5041 NNS denotes cells
T32578 5042-5046 IN denotes with
T32579 5047-5053 NN denotes 5xGAL4
T32581 5053-5054 HYPH denotes -
T32580 5054-5058 NN denotes pGL3
T32583 5059-5067 NN denotes reporter
T32582 5068-5075 NN denotes plasmid
T32584 5075-5076 . denotes .
T32585 5076-5174 sentence denotes Fold repression was calculated as the fold decrease in luciferase activity compared with DBD-mrs.
T32586 5077-5081 NN denotes Fold
T32587 5082-5092 NN denotes repression
T32589 5093-5096 VBD denotes was
T32588 5097-5107 VBN denotes calculated
T32590 5108-5110 IN denotes as
T32591 5111-5114 DT denotes the
T32593 5115-5119 NN denotes fold
T32592 5120-5128 NN denotes decrease
T32594 5129-5131 IN denotes in
T32595 5132-5142 NN denotes luciferase
T32596 5143-5151 NN denotes activity
T32597 5152-5160 VBN denotes compared
T32598 5161-5165 IN denotes with
T32599 5166-5169 NN denotes DBD
T32601 5169-5170 HYPH denotes -
T32600 5170-5173 NN denotes mrs
T32602 5173-5174 . denotes .
T32603 5174-5215 sentence denotes Error bars represent standard deviation.
T32604 5175-5180 NN denotes Error
T32605 5181-5185 NNS denotes bars
T32606 5186-5195 VBP denotes represent
T32607 5196-5204 JJ denotes standard
T32608 5205-5214 NN denotes deviation
T32609 5214-5215 . denotes .
T32610 5215-5310 sentence denotes (D) Various amounts of DBD-tail or DBD-SAM were transfected with 5xGAL4-pGL3 reporter plasmid.
T32611 5216-5217 -LRB- denotes (
T32612 5217-5218 LS denotes D
T32614 5218-5219 -RRB- denotes )
T32615 5220-5227 JJ denotes Various
T32616 5228-5235 NNS denotes amounts
T32617 5236-5238 IN denotes of
T32618 5239-5242 NN denotes DBD
T32620 5242-5243 HYPH denotes -
T32619 5243-5247 NN denotes tail
T32621 5248-5250 CC denotes or
T32622 5251-5254 NN denotes DBD
T32624 5254-5255 HYPH denotes -
T32623 5255-5258 NN denotes SAM
T32625 5259-5263 VBD denotes were
T32613 5264-5275 VBN denotes transfected
T32626 5276-5280 IN denotes with
T32627 5281-5287 NN denotes 5xGAL4
T32629 5287-5288 HYPH denotes -
T32628 5288-5292 NN denotes pGL3
T32631 5293-5301 NN denotes reporter
T32630 5302-5309 NN denotes plasmid
T32632 5309-5310 . denotes .
T32633 5310-5355 sentence denotes Error bars represent standard error of mean.
T32634 5311-5316 NN denotes Error
T32635 5317-5321 NNS denotes bars
T32636 5322-5331 VBP denotes represent
T32637 5332-5340 JJ denotes standard
T32638 5341-5346 NN denotes error
T32639 5347-5349 IN denotes of
T32640 5350-5354 NN denotes mean
T32641 5354-5355 . denotes .
T32642 5355-5478 sentence denotes (E) pcDNA3 or DBD-mrs (5 μg) was co-transfected into Y79 retinoblastoma cells with 0.5 μg of 5xGAL4-pGL3 reporter plasmid.
T32643 5356-5357 -LRB- denotes (
T32644 5357-5358 LS denotes E
T32646 5358-5359 -RRB- denotes )
T32647 5360-5366 NN denotes pcDNA3
T32648 5367-5369 CC denotes or
T32649 5370-5373 NN denotes DBD
T32651 5373-5374 HYPH denotes -
T32650 5374-5377 NN denotes mrs
T32652 5378-5379 -LRB- denotes (
T32654 5379-5380 CD denotes 5
T32653 5381-5383 NN denotes μg
T32655 5383-5384 -RRB- denotes )
T32656 5385-5388 VBD denotes was
T32645 5389-5403 VBN denotes co-transfected
T32657 5404-5408 IN denotes into
T32658 5409-5412 NN denotes Y79
T32660 5413-5427 NN denotes retinoblastoma
T32659 5428-5433 NNS denotes cells
T32661 5434-5438 IN denotes with
T32662 5439-5442 CD denotes 0.5
T32663 5443-5445 NN denotes μg
T32664 5446-5448 IN denotes of
T32665 5449-5455 NN denotes 5xGAL4
T32667 5455-5456 HYPH denotes -
T32666 5456-5460 NN denotes pGL3
T32669 5461-5469 NN denotes reporter
T32668 5470-5477 NN denotes plasmid
T32670 5477-5478 . denotes .
T32671 5478-5547 sentence denotes The reporter activity in the presence of pcDNA3 was designated as 1.
T32672 5479-5482 DT denotes The
T32674 5483-5491 NN denotes reporter
T32673 5492-5500 NN denotes activity
T32676 5501-5503 IN denotes in
T32677 5504-5507 DT denotes the
T32678 5508-5516 NN denotes presence
T32679 5517-5519 IN denotes of
T32680 5520-5526 NN denotes pcDNA3
T32681 5527-5530 VBD denotes was
T32675 5531-5541 VBN denotes designated
T32682 5542-5544 IN denotes as
T32683 5545-5546 CD denotes 1
T32684 5546-5547 . denotes .
T32685 5547-5588 sentence denotes Error bars represent standard deviation.
T32686 5548-5553 NN denotes Error
T32687 5554-5558 NNS denotes bars
T32688 5559-5568 VBP denotes represent
T32689 5569-5577 JJ denotes standard
T32690 5578-5587 NN denotes deviation
T32691 5587-5588 . denotes .
T32692 5588-5670 sentence denotes Asterisk marks statistically significant difference (Student's t test: p < 0.03).
T32693 5589-5597 NN denotes Asterisk
T32694 5598-5603 VBZ denotes marks
T32695 5604-5617 RB denotes statistically
T32696 5618-5629 JJ denotes significant
T32697 5630-5640 NN denotes difference
T32698 5641-5642 -LRB- denotes (
T32700 5642-5649 NNP denotes Student
T32701 5649-5651 POS denotes 's
T32702 5652-5653 NN denotes t
T32699 5654-5658 NN denotes test
T32703 5658-5660 : denotes :
T32704 5660-5661 NN denotes p
T32706 5662-5663 SYM denotes <
T32705 5664-5668 CD denotes 0.03
T32707 5668-5669 -RRB- denotes )
T32708 5669-5670 . denotes .
T32709 5670-5768 sentence denotes (F) Alignment of the C-terminal regions for mouse, rat, human, chick and zebrafish mr-s proteins.
T32710 5671-5672 -LRB- denotes (
T32711 5672-5673 LS denotes F
T32713 5673-5674 -RRB- denotes )
T32712 5675-5684 NN denotes Alignment
T32714 5685-5687 IN denotes of
T32715 5688-5691 DT denotes the
T32717 5692-5693 NN denotes C
T32719 5693-5694 HYPH denotes -
T32718 5694-5702 JJ denotes terminal
T32716 5703-5710 NNS denotes regions
T32720 5711-5714 IN denotes for
T32721 5715-5720 NN denotes mouse
T32723 5720-5722 , denotes ,
T32724 5722-5725 NN denotes rat
T32725 5725-5727 , denotes ,
T32726 5727-5732 JJ denotes human
T32727 5732-5734 , denotes ,
T32728 5734-5739 NN denotes chick
T32729 5740-5743 CC denotes and
T32730 5744-5753 NN denotes zebrafish
T32731 5754-5756 NN denotes mr
T32733 5756-5757 HYPH denotes -
T32732 5757-5758 NN denotes s
T32722 5759-5767 NN denotes proteins
T32734 5767-5768 . denotes .
T32735 5768-5828 sentence denotes Conserved amino acid residues are shown with a dark shadow.
T32736 5769-5778 VBN denotes Conserved
T32738 5779-5784 NN denotes amino
T32739 5785-5789 NN denotes acid
T32737 5790-5798 NNS denotes residues
T32741 5799-5802 VBP denotes are
T32740 5803-5808 VBN denotes shown
T32742 5809-5813 IN denotes with
T32743 5814-5815 DT denotes a
T32745 5816-5820 JJ denotes dark
T32744 5821-5827 NN denotes shadow
T32746 5827-5828 . denotes .
T16969 5829-5834 VBN denotes Taken
T16971 5835-5843 RB denotes together
T16972 5843-5845 , denotes ,
T16973 5845-5848 PRP$ denotes our
T16974 5849-5857 NNS denotes findings
T16970 5858-5865 VBP denotes suggest
T16975 5866-5870 IN denotes that
T16977 5871-5874 NN denotes DBD
T16979 5874-5875 HYPH denotes -
T16978 5875-5878 NN denotes mrs
T16976 5879-5888 VBZ denotes functions
T16980 5889-5891 IN denotes as
T16981 5892-5893 DT denotes a
T16983 5894-5909 JJ denotes transcriptional
T16982 5910-5919 NN denotes repressor
T16984 5920-5923 CC denotes and
T16985 5924-5928 IN denotes that
T16987 5929-5932 DT denotes the
T16989 5933-5943 NN denotes repression
T16988 5944-5952 NN denotes activity
T16990 5953-5955 IN denotes of
T16991 5956-5958 NN denotes mr
T16993 5958-5959 HYPH denotes -
T16992 5959-5960 NN denotes s
T16986 5961-5963 VBZ denotes is
T16994 5964-5967 RB denotes not
T16995 5968-5971 IN denotes due
T16996 5972-5974 IN denotes to
T16997 5975-5976 DT denotes a
T16999 5977-5987 JJ denotes homophilic
T16998 5988-5999 NN denotes interaction
T17000 6000-6007 IN denotes through
T17001 6008-6011 PRP$ denotes its
T17003 6012-6015 NN denotes SAM
T17002 6016-6022 NN denotes domain
T17004 6023-6026 CC denotes but
T17005 6027-6029 IN denotes to
T17006 6030-6033 DT denotes the
T17008 6034-6035 NN denotes C
T17010 6035-6036 HYPH denotes -
T17009 6036-6044 JJ denotes terminal
T17007 6045-6051 NN denotes region
T17011 6052-6053 -LRB- denotes (
T17013 6053-6058 NN denotes amino
T17014 6059-6064 NNS denotes acids
T17012 6065-6068 CD denotes 463
T17015 6069-6071 IN denotes to
T17016 6072-6075 CD denotes 542
T17017 6075-6076 -RRB- denotes )
T17018 6076-6077 . denotes .
R3969 T16058 T16059 det The,protein
R3970 T16059 T16066 nsubj protein,functions
R3971 T16060 T16061 nmod GAL4,fusion
R3972 T16061 T16059 compound fusion,protein
R3973 T16062 T16060 punct -,GAL4
R3974 T16063 T16064 compound mr,s
R3975 T16064 T16060 appos s,GAL4
R3976 T16065 T16064 punct -,s
R3977 T16067 T16066 prep as,functions
R3978 T16068 T16069 amod transcriptional,repressor
R3979 T16069 T16067 pobj repressor,as
R3980 T16071 T16072 det A,member
R3981 T16072 T16073 nsubj member,contain
R3982 T16074 T16072 prep of,member
R3983 T16075 T16076 compound PcG,proteins
R3984 T16076 T16074 pobj proteins,of
R3985 T16077 T16072 punct ", ",member
R3986 T16078 T16072 appos ph,member
R3987 T16079 T16073 punct ", ",contain
R3988 T16080 T16073 aux does,contain
R3989 T16081 T16073 neg not,contain
R3990 T16082 T16083 det an,motif
R3991 T16083 T16073 dobj motif,contain
R3992 T16084 T16083 amod obvious,motif
R3993 T16085 T16086 npadvmod sequence,specific
R3994 T16086 T16083 amod specific,motif
R3995 T16087 T16086 punct -,specific
R3996 T16088 T16089 npadvmod DNA,binding
R3997 T16089 T16083 amod binding,motif
R3998 T16090 T16091 punct (,16
R3999 T16091 T16073 parataxis 16,contain
R4000 T16092 T16091 punct ),16
R4001 T16093 T16073 punct .,contain
R4002 T16095 T16096 nsubj Ph,functions
R4003 T16097 T16096 prep as,functions
R4004 T16098 T16099 det a,repressor
R4005 T16099 T16097 pobj repressor,as
R4006 T16100 T16099 amod transcriptional,repressor
R4007 T16101 T16096 prep through,functions
R4008 T16102 T16103 poss its,polymerization
R4009 T16103 T16101 pobj polymerization,through
R4010 T16104 T16103 cc and,polymerization
R4011 T16105 T16106 compound protein,protein
R4012 T16106 T16108 compound protein,interaction
R4013 T16107 T16106 punct -,protein
R4014 T16108 T16103 conj interaction,polymerization
R4015 T16109 T16108 prep with,interaction
R4016 T16110 T16111 amod other,repressors
R4017 T16111 T16109 pobj repressors,with
R4018 T16112 T16113 npadvmod sequence,specific
R4019 T16113 T16111 amod specific,repressors
R4020 T16114 T16113 punct -,specific
R4021 T16115 T16111 amod transcriptional,repressors
R4022 T16116 T16111 punct ", ",repressors
R4023 T16117 T16118 dep which,form
R4024 T16118 T16111 relcl form,repressors
R4025 T16119 T16118 aux can,form
R4026 T16120 T16121 det a,structure
R4027 T16121 T16118 dobj structure,form
R4028 T16122 T16123 amod higher,order
R4029 T16123 T16121 compound order,structure
R4030 T16124 T16121 compound chromatin,structure
R4031 T16125 T16096 punct .,functions
R4032 T16127 T16128 det The,protein
R4033 T16128 T16132 nsubj protein,have
R4034 T16129 T16130 compound mr,s
R4035 T16130 T16128 compound s,protein
R4036 T16131 T16130 punct -,s
R4037 T16133 T16132 advmod also,have
R4038 T16134 T16132 aux does,have
R4039 T16135 T16132 neg not,have
R4040 T16136 T16137 det an,domain
R4041 T16137 T16132 dobj domain,have
R4042 T16138 T16137 amod obvious,domain
R4043 T16139 T16140 npadvmod DNA,binding
R4044 T16140 T16137 amod binding,domain
R4045 T16141 T16140 punct -,binding
R4046 T16142 T16132 punct .,have
R4047 T16144 T16145 aux To,characterize
R4048 T16145 T16146 advcl characterize,performed
R4049 T16147 T16148 det the,activity
R4050 T16148 T16145 dobj activity,characterize
R4051 T16149 T16148 amod biochemical,activity
R4052 T16150 T16148 prep of,activity
R4053 T16151 T16152 compound mr,s
R4054 T16152 T16150 pobj s,of
R4055 T16153 T16152 punct -,s
R4056 T16154 T16146 punct ", ",performed
R4057 T16155 T16146 nsubj we,performed
R4058 T16156 T16146 advmod next,performed
R4059 T16157 T16158 det a,assay
R4060 T16158 T16146 dobj assay,performed
R4061 T16159 T16158 compound luciferase,assay
R4062 T16160 T16146 punct .,performed
R4063 T16162 T16163 nsubj We,generated
R4064 T16164 T16165 compound effector,plasmids
R4065 T16165 T16163 dobj plasmids,generated
R4066 T16166 T16165 punct ", ",plasmids
R4067 T16167 T16168 dep which,express
R4068 T16168 T16165 relcl express,plasmids
R4069 T16169 T16170 amod various,constructs
R4070 T16170 T16168 dobj constructs,express
R4071 T16171 T16170 compound deletion,constructs
R4072 T16172 T16170 prep of,constructs
R4073 T16173 T16174 compound mr,s
R4074 T16174 T16172 pobj s,of
R4075 T16175 T16174 punct -,s
R4076 T16176 T16170 acl fused,constructs
R4077 T16177 T16176 prep to,fused
R4078 T16178 T16179 det the,domain
R4079 T16179 T16177 pobj domain,to
R4080 T16180 T16179 nmod GAL4,domain
R4081 T16181 T16182 npadvmod DNA,binding
R4082 T16182 T16179 amod binding,domain
R4083 T16183 T16182 punct -,binding
R4084 T16184 T16185 punct (,7A
R4085 T16185 T16163 parataxis 7A,generated
R4086 T16186 T16185 compound Fig.,7A
R4087 T16187 T16185 punct ),7A
R4088 T16188 T16163 punct .,generated
R4089 T16190 T16191 nsubj We,confirmed
R4090 T16192 T16191 advmod first,confirmed
R4091 T16193 T16194 mark that,had
R4092 T16194 T16191 ccomp had,confirmed
R4093 T16195 T16196 amod full,length
R4094 T16196 T16198 compound length,s
R4095 T16197 T16196 punct -,length
R4096 T16198 T16194 nsubj s,had
R4097 T16199 T16198 compound mr,s
R4098 T16200 T16198 punct -,s
R4099 T16201 T16198 acl fused,s
R4100 T16202 T16201 prep to,fused
R4101 T16203 T16204 compound GAL4,domain
R4102 T16204 T16202 pobj domain,to
R4103 T16205 T16206 compound DNA,binding
R4104 T16206 T16204 compound binding,domain
R4105 T16207 T16204 punct (,domain
R4106 T16208 T16209 compound DBD,mrs
R4107 T16209 T16204 appos mrs,domain
R4108 T16210 T16209 punct -,mrs
R4109 T16211 T16194 punct ),had
R4110 T16212 T16213 det no,effect
R4111 T16213 T16194 dobj effect,had
R4112 T16214 T16213 prep on,effect
R4113 T16215 T16216 det the,plasmid
R4114 T16216 T16214 pobj plasmid,on
R4115 T16217 T16216 compound pGL3,plasmid
R4116 T16218 T16216 compound promoter,plasmid
R4117 T16219 T16216 acl lacking,plasmid
R4118 T16220 T16221 compound GAL4,binding
R4119 T16221 T16222 compound binding,sites
R4120 T16222 T16219 dobj sites,lacking
R4121 T16223 T16224 punct (,shown
R4122 T16224 T16191 parataxis shown,confirmed
R4123 T16225 T16224 nsubj data,shown
R4124 T16226 T16224 neg not,shown
R4125 T16227 T16224 punct ),shown
R4126 T16228 T16191 punct .,confirmed
R4127 T16230 T16231 advmod When,co-transfected
R4128 T16231 T16239 advcl co-transfected,repressed
R4129 T16232 T16233 det the,plasmid
R4130 T16233 T16231 nsubjpass plasmid,co-transfected
R4131 T16234 T16235 compound 5xGAL4,pGL3
R4132 T16235 T16233 compound pGL3,plasmid
R4133 T16236 T16235 punct -,pGL3
R4134 T16237 T16233 compound reporter,plasmid
R4135 T16238 T16231 auxpass was,co-transfected
R4136 T16240 T16239 punct ", ",repressed
R4137 T16241 T16242 compound DBD,mrs
R4138 T16242 T16239 nsubj mrs,repressed
R4139 T16243 T16242 punct -,mrs
R4140 T16244 T16245 compound luciferase,activity
R4141 T16245 T16239 dobj activity,repressed
R4142 T16246 T16239 prep by,repressed
R4143 T16247 T16248 quantmod about,90
R4144 T16248 T16249 nummod 90,%
R4145 T16249 T16246 pobj %,by
R4146 T16250 T16239 prep in,repressed
R4147 T16251 T16252 det a,manner
R4148 T16252 T16250 pobj manner,in
R4149 T16253 T16254 npadvmod dose,dependent
R4150 T16254 T16252 amod dependent,manner
R4151 T16255 T16254 punct -,dependent
R4152 T16256 T16257 punct (,7B
R4153 T16257 T16239 parataxis 7B,repressed
R4154 T16258 T16257 compound Fig.,7B
R4155 T16259 T16257 punct ),7B
R4156 T16260 T16239 punct .,repressed
R4157 T16262 T16263 prep As,had
R4158 T16264 T16265 det a,control
R4159 T16265 T16262 pobj control,As
R4160 T16266 T16263 punct ", ",had
R4161 T16267 T16268 nmod GAL4,domain
R4162 T16268 T16263 nsubj domain,had
R4163 T16269 T16270 npadvmod DNA,binding
R4164 T16270 T16268 amod binding,domain
R4165 T16271 T16270 punct -,binding
R4166 T16272 T16268 punct (,domain
R4167 T16273 T16268 appos DBD,domain
R4168 T16274 T16263 punct ),had
R4169 T16275 T16276 det no,effect
R4170 T16276 T16263 dobj effect,had
R4171 T16277 T16276 amod significant,effect
R4172 T16278 T16276 prep on,effect
R4173 T16279 T16280 det the,plasmid
R4174 T16280 T16278 pobj plasmid,on
R4175 T16281 T16282 compound 5xGAL4,pGL3
R4176 T16282 T16280 compound pGL3,plasmid
R4177 T16283 T16282 punct -,pGL3
R4178 T16284 T16280 compound reporter,plasmid
R4179 T16285 T16263 punct .,had
R4180 T16287 T16288 prep In,confirmed
R4181 T16289 T16287 pobj addition,In
R4182 T16290 T16288 punct ", ",confirmed
R4183 T16291 T16288 nsubj we,confirmed
R4184 T16292 T16293 mark that,had
R4185 T16293 T16288 ccomp had,confirmed
R4186 T16294 T16295 amod full,length
R4187 T16295 T16297 compound length,s
R4188 T16296 T16295 punct -,length
R4189 T16297 T16293 nsubj s,had
R4190 T16298 T16297 compound mr,s
R4191 T16299 T16297 punct -,s
R4192 T16300 T16297 prep without,s
R4193 T16301 T16302 compound GAL4,DBD
R4194 T16302 T16300 pobj DBD,without
R4195 T16303 T16304 det no,effect
R4196 T16304 T16293 dobj effect,had
R4197 T16305 T16304 prep on,effect
R4198 T16306 T16307 det the,plasmid
R4199 T16307 T16305 pobj plasmid,on
R4200 T16308 T16307 amod same,plasmid
R4201 T16309 T16307 compound reporter,plasmid
R4202 T16310 T16311 punct (,shown
R4203 T16311 T16288 parataxis shown,confirmed
R4204 T16312 T16311 nsubj data,shown
R4205 T16313 T16311 neg not,shown
R4206 T16314 T16311 punct ),shown
R4207 T16315 T16288 punct .,confirmed
R4208 T16317 T16318 nsubj We,analyzed
R4209 T16319 T16318 advmod next,analyzed
R4210 T16320 T16321 compound deletion,constructs
R4211 T16321 T16318 dobj constructs,analyzed
R4212 T16322 T16323 prep in,fused
R4213 T16323 T16321 relcl fused,constructs
R4214 T16324 T16322 pobj which,in
R4215 T16325 T16326 det the,stretch
R4216 T16326 T16323 nsubjpass stretch,fused
R4217 T16327 T16328 nmod N,terminus
R4218 T16328 T16326 nmod terminus,stretch
R4219 T16329 T16328 punct -,terminus
R4220 T16330 T16331 nummod 400,aa
R4221 T16331 T16326 compound aa,stretch
R4222 T16332 T16326 prep of,stretch
R4223 T16333 T16334 compound mr,s
R4224 T16334 T16332 pobj s,of
R4225 T16335 T16334 punct -,s
R4226 T16336 T16337 punct (,1
R4227 T16337 T16326 parataxis 1,stretch
R4228 T16338 T16337 nmod amino,1
R4229 T16339 T16337 nmod acids,1
R4230 T16340 T16337 prep to,1
R4231 T16341 T16340 pobj 400,to
R4232 T16342 T16337 punct ),1
R4233 T16343 T16326 cc or,stretch
R4234 T16344 T16345 det the,portion
R4235 T16345 T16326 conj portion,stretch
R4236 T16346 T16347 compound C,terminus
R4237 T16347 T16345 compound terminus,portion
R4238 T16348 T16347 punct -,terminus
R4239 T16349 T16350 punct (,391
R4240 T16350 T16345 parataxis 391,portion
R4241 T16351 T16350 nmod amino,391
R4242 T16352 T16350 nmod acids,391
R4243 T16353 T16350 prep to,391
R4244 T16354 T16353 pobj 542,to
R4245 T16355 T16350 punct ),391
R4246 T16356 T16323 auxpass were,fused
R4247 T16357 T16323 prep to,fused
R4248 T16358 T16359 det the,DBD
R4249 T16359 T16357 pobj DBD,to
R4250 T16360 T16359 compound GAL4,DBD
R4251 T16361 T16362 punct (,7A
R4252 T16362 T16323 parataxis 7A,fused
R4253 T16363 T16362 compound Fig.,7A
R4254 T16364 T16362 punct ", ",7A
R4255 T16365 T16366 compound DBD,N
R4256 T16366 T16362 appos N,7A
R4257 T16367 T16366 punct -,N
R4258 T16368 T16362 punct ", ",7A
R4259 T16369 T16370 compound DBD,C
R4260 T16370 T16362 appos C,7A
R4261 T16371 T16370 punct -,C
R4262 T16372 T16362 punct ),7A
R4263 T16373 T16318 punct .,analyzed
R4264 T16375 T16376 mark While,had
R4265 T16376 T16380 advcl had,repressed
R4266 T16377 T16378 compound DBD,N
R4267 T16378 T16376 nsubj N,had
R4268 T16379 T16378 punct -,N
R4269 T16381 T16382 det no,effect
R4270 T16382 T16376 dobj effect,had
R4271 T16383 T16382 amod repressive,effect
R4272 T16384 T16382 prep on,effect
R4273 T16385 T16386 det this,activity
R4274 T16386 T16384 pobj activity,on
R4275 T16387 T16386 compound reporter,activity
R4276 T16388 T16380 punct ", ",repressed
R4277 T16389 T16390 compound DBD,C
R4278 T16390 T16380 nsubj C,repressed
R4279 T16391 T16390 punct -,C
R4280 T16392 T16393 compound luciferase,expression
R4281 T16393 T16380 dobj expression,repressed
R4282 T16394 T16393 compound gene,expression
R4283 T16395 T16380 prep by,repressed
R4284 T16396 T16397 quantmod about,65
R4285 T16397 T16398 nummod 65,%
R4286 T16398 T16395 pobj %,by
R4287 T16399 T16380 punct .,repressed
R4288 T16401 T16402 det This,result
R4289 T16402 T16403 nsubj result,suggested
R4290 T16404 T16403 prep to,suggested
R4291 T16405 T16404 pobj us,to
R4292 T16406 T16407 det the,possibility
R4293 T16407 T16403 dobj possibility,suggested
R4294 T16408 T16409 mark that,exerts
R4295 T16409 T16407 acl exerts,possibility
R4296 T16410 T16411 compound DBD,mrs
R4297 T16411 T16409 nsubj mrs,exerts
R4298 T16412 T16411 punct -,mrs
R4299 T16413 T16414 amod transcriptional,activity
R4300 T16414 T16409 dobj activity,exerts
R4301 T16415 T16414 amod repressive,activity
R4302 T16416 T16409 prep via,exerts
R4303 T16417 T16418 compound self,association
R4304 T16418 T16416 pobj association,via
R4305 T16419 T16418 punct -,association
R4306 T16420 T16409 prep through,exerts
R4307 T16421 T16422 poss its,domain
R4308 T16422 T16420 pobj domain,through
R4309 T16423 T16422 compound SAM,domain
R4310 T16424 T16403 punct .,suggested
R4311 T16426 T16427 aux To,investigate
R4312 T16427 T16428 advcl investigate,analyzed
R4313 T16429 T16430 mark whether,required
R4314 T16430 T16427 ccomp required,investigate
R4315 T16431 T16432 det the,association
R4316 T16432 T16430 nsubjpass association,required
R4317 T16433 T16432 amod homophilic,association
R4318 T16434 T16432 prep of,association
R4319 T16435 T16436 compound mr,s
R4320 T16436 T16434 pobj s,of
R4321 T16437 T16436 punct -,s
R4322 T16438 T16430 auxpass is,required
R4323 T16439 T16430 prep for,required
R4324 T16440 T16441 amod transcriptional,repression
R4325 T16441 T16439 pobj repression,for
R4326 T16442 T16428 punct ", ",analyzed
R4327 T16443 T16444 nummod two,mutants
R4328 T16444 T16428 nsubjpass mutants,analyzed
R4329 T16445 T16446 npadvmod site,directed
R4330 T16446 T16444 amod directed,mutants
R4331 T16447 T16446 punct -,directed
R4332 T16448 T16444 punct ", ",mutants
R4333 T16449 T16450 compound DBD,W404A
R4334 T16450 T16444 appos W404A,mutants
R4335 T16451 T16450 punct -,W404A
R4336 T16452 T16450 cc and,W404A
R4337 T16453 T16454 compound DBD,G453A
R4338 T16454 T16450 conj G453A,W404A
R4339 T16455 T16454 punct -,G453A
R4340 T16456 T16444 punct ", ",mutants
R4341 T16457 T16458 dep either,reduce
R4342 T16458 T16444 relcl reduce,mutants
R4343 T16459 T16457 prep of,either
R4344 T16460 T16459 pobj which,of
R4345 T16461 T16458 aux may,reduce
R4346 T16462 T16463 npadvmod self,binding
R4347 T16463 T16465 amod binding,ability
R4348 T16464 T16463 punct -,binding
R4349 T16465 T16458 dobj ability,reduce
R4350 T16466 T16428 punct ", ",analyzed
R4351 T16467 T16428 auxpass were,analyzed
R4352 T16468 T16469 punct (,7C
R4353 T16469 T16428 parataxis 7C,analyzed
R4354 T16470 T16469 compound Fig.,7C
R4355 T16471 T16469 punct ),7C
R4356 T16472 T16428 punct .,analyzed
R4357 T16474 T16475 prep Compared,had
R4358 T16476 T16474 prep to,Compared
R4359 T16477 T16478 compound DBD,mrs
R4360 T16478 T16476 pobj mrs,to
R4361 T16479 T16478 punct -,mrs
R4362 T16480 T16475 punct ", ",had
R4363 T16481 T16482 compound DBD,W404A
R4364 T16482 T16475 nsubj W404A,had
R4365 T16483 T16482 punct -,W404A
R4366 T16484 T16482 cc and,W404A
R4367 T16485 T16486 compound DBD,G453A
R4368 T16486 T16482 conj G453A,W404A
R4369 T16487 T16486 punct -,G453A
R4370 T16488 T16489 compound repression,activity
R4371 T16489 T16475 dobj activity,had
R4372 T16490 T16489 prep of,activity
R4373 T16491 T16492 nummod 72,%
R4374 T16492 T16490 pobj %,of
R4375 T16493 T16492 cc and,%
R4376 T16494 T16495 nummod 87,%
R4377 T16495 T16492 conj %,%
R4378 T16496 T16475 punct ", ",had
R4379 T16497 T16475 advmod respectively,had
R4380 T16498 T16475 punct .,had
R4381 T16500 T16501 mark While,correlates
R4382 T16501 T16512 advcl correlates,compromise
R4383 T16502 T16503 det the,ability
R4384 T16503 T16501 nsubj ability,correlates
R4385 T16504 T16503 prep of,ability
R4386 T16505 T16506 compound mr,s
R4387 T16506 T16508 compound s,association
R4388 T16507 T16506 punct -,s
R4389 T16508 T16504 pobj association,of
R4390 T16509 T16508 compound self,association
R4391 T16510 T16508 punct -,association
R4392 T16511 T16501 advmod partially,correlates
R4393 T16513 T16501 prep with,correlates
R4394 T16514 T16515 amod transcriptional,activity
R4395 T16515 T16513 pobj activity,with
R4396 T16516 T16515 amod repressive,activity
R4397 T16517 T16512 punct ", ",compromise
R4398 T16518 T16519 det these,mutants
R4399 T16519 T16512 nsubj mutants,compromise
R4400 T16520 T16512 aux do,compromise
R4401 T16521 T16512 neg not,compromise
R4402 T16522 T16523 det the,ability
R4403 T16523 T16512 dobj ability,compromise
R4404 T16524 T16525 aux to,repress
R4405 T16525 T16523 acl repress,ability
R4406 T16526 T16525 dobj transcription,repress
R4407 T16527 T16525 advmod critically,repress
R4408 T16528 T16512 punct .,compromise
R4409 T16530 T16531 aux To,determine
R4410 T16531 T16532 advcl determine,divided
R4411 T16533 T16534 det the,regions
R4412 T16534 T16531 dobj regions,determine
R4413 T16535 T16534 prep of,regions
R4414 T16536 T16537 compound mr,s
R4415 T16537 T16535 pobj s,of
R4416 T16538 T16537 punct -,s
R4417 T16539 T16534 acl involved,regions
R4418 T16540 T16539 prep in,involved
R4419 T16541 T16542 amod transcriptional,repression
R4420 T16542 T16540 pobj repression,in
R4421 T16543 T16544 advmod more,precisely
R4422 T16544 T16531 advmod precisely,determine
R4423 T16545 T16532 punct ", ",divided
R4424 T16546 T16547 det the,portion
R4425 T16547 T16532 nsubjpass portion,divided
R4426 T16548 T16549 compound C,terminus
R4427 T16549 T16547 compound terminus,portion
R4428 T16550 T16549 punct -,terminus
R4429 T16551 T16532 auxpass was,divided
R4430 T16552 T16532 prep into,divided
R4431 T16553 T16554 nummod two,regions
R4432 T16554 T16552 pobj regions,into
R4433 T16555 T16532 cc and,divided
R4434 T16556 T16557 nsubjpass each,fused
R4435 T16557 T16532 conj fused,divided
R4436 T16558 T16557 auxpass was,fused
R4437 T16559 T16557 prep to,fused
R4438 T16560 T16561 det the,DBD
R4439 T16561 T16559 pobj DBD,to
R4440 T16562 T16561 compound GAL4,DBD
R4441 T16563 T16564 punct (,SAM
R4442 T16564 T16557 parataxis SAM,fused
R4443 T16565 T16566 compound Fig.,7A
R4444 T16566 T16564 dep 7A,SAM
R4445 T16567 T16564 punct ", ",SAM
R4446 T16568 T16569 compound DBD,tail
R4447 T16569 T16564 dep tail,SAM
R4448 T16570 T16569 punct -,tail
R4449 T16571 T16564 punct ", ",SAM
R4450 T16572 T16564 compound DBD,SAM
R4451 T16573 T16564 punct -,SAM
R4452 T16574 T16564 punct ),SAM
R4453 T16575 T16532 punct .,divided
R4454 T16577 T16578 prep As,have
R4455 T16579 T16580 det a,consequence
R4456 T16580 T16577 pobj consequence,As
R4457 T16581 T16578 punct ", ",have
R4458 T16582 T16583 compound DBD,SAM
R4459 T16583 T16578 nsubj SAM,have
R4460 T16584 T16583 punct -,SAM
R4461 T16585 T16586 punct (,384
R4462 T16586 T16583 parataxis 384,SAM
R4463 T16587 T16586 nmod amino,384
R4464 T16588 T16586 nmod acids,384
R4465 T16589 T16586 prep to,384
R4466 T16590 T16589 pobj 462,to
R4467 T16591 T16586 punct ),384
R4468 T16592 T16578 aux did,have
R4469 T16593 T16578 neg not,have
R4470 T16594 T16595 det a,effect
R4471 T16595 T16578 dobj effect,have
R4472 T16596 T16595 amod repressive,effect
R4473 T16597 T16595 prep on,effect
R4474 T16598 T16599 det the,plasmid
R4475 T16599 T16597 pobj plasmid,on
R4476 T16600 T16601 compound 5xGAL4,pGL3
R4477 T16601 T16599 compound pGL3,plasmid
R4478 T16602 T16601 punct -,pGL3
R4479 T16603 T16599 compound reporter,plasmid
R4480 T16604 T16578 punct .,have
R4481 T16606 T16607 prep On,repressed
R4482 T16608 T16609 det the,hand
R4483 T16609 T16606 pobj hand,On
R4484 T16610 T16609 amod other,hand
R4485 T16611 T16607 punct ", ",repressed
R4486 T16612 T16613 compound luciferase,activity
R4487 T16613 T16607 nsubjpass activity,repressed
R4488 T16614 T16607 auxpass was,repressed
R4489 T16615 T16607 prep by,repressed
R4490 T16616 T16617 nummod 55,%
R4491 T16617 T16615 pobj %,by
R4492 T16618 T16619 advmod when,co-transfected
R4493 T16619 T16607 advcl co-transfected,repressed
R4494 T16620 T16621 compound DBD,tail
R4495 T16621 T16619 nsubjpass tail,co-transfected
R4496 T16622 T16621 punct -,tail
R4497 T16623 T16624 punct (,459
R4498 T16624 T16621 parataxis 459,tail
R4499 T16625 T16624 nmod amino,459
R4500 T16626 T16624 nmod acids,459
R4501 T16627 T16624 prep to,459
R4502 T16628 T16627 pobj 542,to
R4503 T16629 T16624 punct ),459
R4504 T16630 T16619 auxpass was,co-transfected
R4505 T16631 T16619 prep with,co-transfected
R4506 T16632 T16633 det this,plasmid
R4507 T16633 T16631 pobj plasmid,with
R4508 T16634 T16633 compound reporter,plasmid
R4509 T16635 T16636 punct (,7D
R4510 T16636 T16619 parataxis 7D,co-transfected
R4511 T16637 T16636 compound Fig.,7D
R4512 T16638 T16636 punct ),7D
R4513 T16639 T16607 punct .,repressed
R4514 T16641 T16642 aux To,assess
R4515 T16642 T16643 advcl assess,performed
R4516 T16644 T16645 det the,activity
R4517 T16645 T16642 dobj activity,assess
R4518 T16646 T16645 amod transcriptional,activity
R4519 T16647 T16645 amod repressive,activity
R4520 T16648 T16645 prep of,activity
R4521 T16649 T16650 compound mr,s
R4522 T16650 T16648 pobj s,of
R4523 T16651 T16650 punct -,s
R4524 T16652 T16642 prep in,assess
R4525 T16653 T16652 pobj cells,in
R4526 T16654 T16653 prep of,cells
R4527 T16655 T16656 amod retinal,origin
R4528 T16656 T16654 pobj origin,of
R4529 T16657 T16643 punct ", ",performed
R4530 T16658 T16643 nsubj we,performed
R4531 T16659 T16660 amod similar,experiments
R4532 T16660 T16643 dobj experiments,performed
R4533 T16661 T16643 advcl using,performed
R4534 T16662 T16663 amod human,cells
R4535 T16663 T16661 dobj cells,using
R4536 T16664 T16663 compound Y79,cells
R4537 T16665 T16663 compound retinoblastoma,cells
R4538 T16666 T16643 punct .,performed
R4539 T16668 T16669 det The,results
R4540 T16669 T16670 nsubj results,indicated
R4541 T16671 T16672 mark that,reduced
R4542 T16672 T16670 ccomp reduced,indicated
R4543 T16673 T16674 compound DBD,mrs
R4544 T16674 T16672 nsubj mrs,reduced
R4545 T16675 T16674 punct -,mrs
R4546 T16676 T16672 advmod also,reduced
R4547 T16677 T16678 compound luciferase,activity
R4548 T16678 T16672 dobj activity,reduced
R4549 T16679 T16672 advmod significantly,reduced
R4550 T16680 T16672 prep in,reduced
R4551 T16681 T16682 compound Y79,cells
R4552 T16682 T16680 pobj cells,in
R4553 T16683 T16682 compound retinoblastoma,cells
R4554 T16684 T16685 punct (,7E
R4555 T16685 T16672 parataxis 7E,reduced
R4556 T16686 T16685 compound Fig.,7E
R4557 T16687 T16685 punct ),7E
R4558 T16688 T16670 punct .,indicated
R4559 T16690 T16691 advmod However,repressed
R4560 T16692 T16691 punct ", ",repressed
R4561 T16693 T16694 compound luciferase,activity
R4562 T16694 T16691 nsubjpass activity,repressed
R4563 T16695 T16691 auxpass was,repressed
R4564 T16696 T16691 prep by,repressed
R4565 T16697 T16698 quantmod about,30
R4566 T16698 T16699 nummod 30,%
R4567 T16699 T16696 pobj %,by
R4568 T16700 T16691 prep in,repressed
R4569 T16701 T16702 compound Y79,cells
R4570 T16702 T16700 pobj cells,in
R4571 T16703 T16691 punct ", ",repressed
R4572 T16704 T16705 mark while,repressed
R4573 T16705 T16691 advcl repressed,repressed
R4574 T16706 T16705 nsubjpass it,repressed
R4575 T16707 T16705 auxpass was,repressed
R4576 T16708 T16705 prep by,repressed
R4577 T16709 T16710 nummod 90,%
R4578 T16710 T16708 pobj %,by
R4579 T16711 T16705 prep in,repressed
R4580 T16712 T16713 compound HEK293T,cells
R4581 T16713 T16711 pobj cells,in
R4582 T16714 T16691 punct .,repressed
R4583 T16716 T16717 nsubj This,be
R4584 T16718 T16717 aux might,be
R4585 T16719 T16717 prep due,be
R4586 T16720 T16719 pcomp to,due
R4587 T16721 T16722 det the,difference
R4588 T16722 T16719 pobj difference,due
R4589 T16723 T16722 prep in,difference
R4590 T16724 T16725 compound transfection,efficiency
R4591 T16725 T16723 pobj efficiency,in
R4592 T16726 T16722 prep between,difference
R4593 T16727 T16728 det these,lines
R4594 T16728 T16726 pobj lines,between
R4595 T16729 T16728 compound cell,lines
R4596 T16730 T16717 punct .,be
R4597 T16732 T16733 det Another,possibility
R4598 T16733 T16734 nsubj possibility,is
R4599 T16735 T16736 mark that,is
R4600 T16736 T16734 ccomp is,is
R4601 T16737 T16738 amod intracellular,environment
R4602 T16738 T16736 nsubj environment,is
R4603 T16739 T16738 prep in,environment
R4604 T16740 T16741 compound Y79,cells
R4605 T16741 T16739 pobj cells,in
R4606 T16742 T16741 punct ", ",cells
R4607 T16743 T16744 det a,line
R4608 T16744 T16741 appos line,cells
R4609 T16745 T16744 compound retinoblastoma,line
R4610 T16746 T16744 compound cell,line
R4611 T16747 T16736 punct ", ",is
R4612 T16748 T16736 acomp insufficient,is
R4613 T16749 T16748 prep for,insufficient
R4614 T16750 T16749 pcomp recapitulating,for
R4615 T16751 T16752 amod developing,photoreceptors
R4616 T16752 T16750 dobj photoreceptors,recapitulating
R4617 T16753 T16734 punct .,is
R4618 T16755 T16756 prep In,address
R4619 T16757 T16758 det this,study
R4620 T16758 T16755 pobj study,In
R4621 T16759 T16756 punct ", ",address
R4622 T16760 T16756 nsubj we,address
R4623 T16761 T16756 aux did,address
R4624 T16762 T16756 neg not,address
R4625 T16763 T16764 det the,question
R4626 T16764 T16756 dobj question,address
R4627 T16765 T16766 mark whether,reflect
R4628 T16766 T16764 acl reflect,question
R4629 T16767 T16766 cc or,reflect
R4630 T16768 T16766 neg not,reflect
R4631 T16769 T16770 poss our,data
R4632 T16770 T16766 nsubj data,reflect
R4633 T16771 T16772 advmod in,vitro
R4634 T16772 T16770 amod vitro,data
R4635 T16773 T16774 amod native,repression
R4636 T16774 T16766 dobj repression,reflect
R4637 T16775 T16776 nmod mr,s
R4638 T16776 T16774 nmod s,repression
R4639 T16777 T16776 punct -,s
R4640 T16778 T16774 amod transcriptional,repression
R4641 T16779 T16780 advmod in,vivo
R4642 T16780 T16766 advmod vivo,reflect
R4643 T16781 T16756 punct .,address
R4644 T16783 T16784 advmod However,support
R4645 T16785 T16784 punct ", ",support
R4646 T16786 T16787 det these,experiments
R4647 T16787 T16784 nsubj experiments,support
R4648 T16788 T16789 advmod in,vitro
R4649 T16789 T16787 amod vitro,experiments
R4650 T16790 T16787 acl using,experiments
R4651 T16791 T16792 nmod HEK293T,cells
R4652 T16792 T16790 dobj cells,using
R4653 T16793 T16791 cc and,HEK293T
R4654 T16794 T16791 conj Y79,HEK293T
R4655 T16795 T16784 advmod strongly,support
R4656 T16796 T16797 poss our,hypothesis
R4657 T16797 T16784 dobj hypothesis,support
R4658 T16798 T16799 mark that,functions
R4659 T16799 T16797 acl functions,hypothesis
R4660 T16800 T16801 compound mr,s
R4661 T16801 T16799 nsubj s,functions
R4662 T16802 T16801 punct -,s
R4663 T16803 T16799 prep as,functions
R4664 T16804 T16805 det a,repressor
R4665 T16805 T16803 pobj repressor,as
R4666 T16806 T16805 amod transcriptional,repressor
R4667 T16807 T16799 prep in,functions
R4668 T16808 T16809 amod developing,photoreceptors
R4669 T16809 T16807 pobj photoreceptors,in
R4670 T16810 T16784 punct .,support
R4671 T16812 T16813 poss Our,results
R4672 T16813 T16814 nsubj results,suggested
R4673 T16815 T16816 mark that,required
R4674 T16816 T16814 ccomp required,suggested
R4675 T16817 T16818 det the,region
R4676 T16818 T16816 nsubjpass region,required
R4677 T16819 T16820 npadvmod C,terminal
R4678 T16820 T16818 amod terminal,region
R4679 T16821 T16820 punct -,terminal
R4680 T16822 T16818 prep of,region
R4681 T16823 T16824 compound mr,s
R4682 T16824 T16822 pobj s,of
R4683 T16825 T16824 punct -,s
R4684 T16826 T16827 punct (,463
R4685 T16827 T16818 parataxis 463,region
R4686 T16828 T16827 nmod amino,463
R4687 T16829 T16827 nmod acids,463
R4688 T16830 T16827 prep to,463
R4689 T16831 T16830 pobj 542,to
R4690 T16832 T16827 punct ),463
R4691 T16833 T16816 auxpass is,required
R4692 T16834 T16816 prep for,required
R4693 T16835 T16836 amod transcriptional,repression
R4694 T16836 T16834 pobj repression,for
R4695 T16837 T16836 prep of,repression
R4696 T16838 T16839 compound mr,s
R4697 T16839 T16837 pobj s,of
R4698 T16840 T16839 punct -,s
R4699 T16841 T16816 cc and,required
R4700 T16842 T16843 det the,domain
R4701 T16843 T16845 nsubj domain,appears
R4702 T16844 T16843 compound SAM,domain
R4703 T16845 T16816 conj appears,required
R4704 T16846 T16847 aux to,be
R4705 T16847 T16845 xcomp be,appears
R4706 T16848 T16847 acomp dispensable,be
R4707 T16849 T16848 prep for,dispensable
R4708 T16850 T16851 det this,activity
R4709 T16851 T16849 pobj activity,for
R4710 T16852 T16851 amod repressive,activity
R4711 T16853 T16814 punct .,suggested
R4712 T16855 T16856 det This,region
R4713 T16856 T16860 nsubjpass region,conserved
R4714 T16857 T16858 npadvmod C,terminal
R4715 T16858 T16856 amod terminal,region
R4716 T16859 T16858 punct -,terminal
R4717 T16861 T16856 prep of,region
R4718 T16862 T16863 compound mouse,s
R4719 T16863 T16861 pobj s,of
R4720 T16864 T16863 compound mr,s
R4721 T16865 T16863 punct -,s
R4722 T16866 T16860 auxpass is,conserved
R4723 T16867 T16860 advmod highly,conserved
R4724 T16868 T16860 prep among,conserved
R4725 T16869 T16868 pobj species,among
R4726 T16870 T16871 punct (,7F
R4727 T16871 T16860 parataxis 7F,conserved
R4728 T16872 T16871 compound Fig.,7F
R4729 T16873 T16871 punct ),7F
R4730 T16874 T16860 punct .,conserved
R4731 T16876 T16877 det The,identity
R4732 T16877 T16879 nsubj identity,was
R4733 T16878 T16877 compound sequence,identity
R4734 T16880 T16877 prep of,identity
R4735 T16881 T16882 det the,region
R4736 T16882 T16880 pobj region,of
R4737 T16883 T16884 nummod 93,%
R4738 T16884 T16879 attr %,was
R4739 T16885 T16884 punct ", ",%
R4740 T16886 T16887 nummod 73,%
R4741 T16887 T16884 conj %,%
R4742 T16888 T16887 punct ", ",%
R4743 T16889 T16890 nummod 41,%
R4744 T16890 T16887 conj %,%
R4745 T16891 T16890 cc and,%
R4746 T16892 T16893 nummod 40,%
R4747 T16893 T16890 conj %,%
R4748 T16894 T16884 prep for,%
R4749 T16895 T16894 pobj rat,for
R4750 T16896 T16895 punct ", ",rat
R4751 T16897 T16895 amod human,rat
R4752 T16898 T16895 punct ", ",rat
R4753 T16899 T16895 conj chick,rat
R4754 T16900 T16899 cc and,chick
R4755 T16901 T16899 conj zebrafish,chick
R4756 T16902 T16884 punct ", ",%
R4757 T16903 T16884 advmod respectively,%
R4758 T16904 T16905 punct (,1D
R4759 T16905 T16879 parataxis 1D,was
R4760 T16906 T16905 compound Fig.,1D
R4761 T16907 T16905 punct ),1D
R4762 T16908 T16879 punct .,was
R4763 T16910 T16911 nsubj This,suggests
R4764 T16912 T16911 advmod strongly,suggests
R4765 T16913 T16914 mark that,functions
R4766 T16914 T16911 ccomp functions,suggests
R4767 T16915 T16916 det the,region
R4768 T16916 T16914 nsubj region,functions
R4769 T16917 T16918 npadvmod C,terminal
R4770 T16918 T16916 amod terminal,region
R4771 T16919 T16918 punct -,terminal
R4772 T16920 T16916 prep of,region
R4773 T16921 T16922 compound mouse,s
R4774 T16922 T16920 pobj s,of
R4775 T16923 T16922 compound mr,s
R4776 T16924 T16922 punct -,s
R4777 T16925 T16914 prep as,functions
R4778 T16926 T16927 det a,domain
R4779 T16927 T16925 pobj domain,as
R4780 T16928 T16927 amod transcriptional,domain
R4781 T16929 T16927 amod repressive,domain
R4782 T16930 T16927 prep in,domain
R4783 T16931 T16932 compound photoreceptor,development
R4784 T16932 T16930 pobj development,in
R4785 T16933 T16911 punct .,suggests
R4786 T16935 T16936 advmod However,contain
R4787 T16937 T16936 punct ", ",contain
R4788 T16938 T16939 det this,region
R4789 T16939 T16936 nsubj region,contain
R4790 T16940 T16936 aux does,contain
R4791 T16941 T16936 neg not,contain
R4792 T16942 T16936 amod characteristic,contain
R4793 T16943 T16944 compound amino,acid
R4794 T16944 T16936 dobj acid,contain
R4795 T16945 T16936 dep motifs,contain
R4796 T16946 T16936 cc and,contain
R4797 T16947 T16948 det the,mechanism
R4798 T16948 T16949 nsubj mechanism,remains
R4799 T16949 T16936 conj remains,contain
R4800 T16950 T16951 prep through,achieves
R4801 T16951 T16948 relcl achieves,mechanism
R4802 T16952 T16950 pobj which,through
R4803 T16953 T16954 det the,region
R4804 T16954 T16951 nsubj region,achieves
R4805 T16955 T16951 cc and,achieves
R4806 T16956 T16955 punct /,and
R4807 T16957 T16955 cc or,and
R4808 T16958 T16951 conj maintains,achieves
R4809 T16959 T16960 compound gene,repression
R4810 T16960 T16958 dobj repression,maintains
R4811 T16961 T16962 aux to,clarified
R4812 T16962 T16949 xcomp clarified,remains
R4813 T16963 T16962 auxpass be,clarified
R4814 T16964 T16962 prep in,clarified
R4815 T16965 T16966 det the,future
R4816 T16966 T16964 pobj future,in
R4817 T16967 T16936 punct .,contain
R4818 T16969 T16970 advcl Taken,suggest
R4819 T16971 T16969 advmod together,Taken
R4820 T16972 T16970 punct ", ",suggest
R4821 T16973 T16974 poss our,findings
R4822 T16974 T16970 nsubj findings,suggest
R4823 T16975 T16976 mark that,functions
R4824 T16976 T16970 advcl functions,suggest
R4825 T16977 T16978 compound DBD,mrs
R4826 T16978 T16976 nsubj mrs,functions
R4827 T16979 T16978 punct -,mrs
R4828 T16980 T16976 prep as,functions
R4829 T16981 T16982 det a,repressor
R4830 T16982 T16980 pobj repressor,as
R4831 T16983 T16982 amod transcriptional,repressor
R4832 T16984 T16976 cc and,functions
R4833 T16985 T16986 mark that,is
R4834 T16986 T16976 conj is,functions
R4835 T16987 T16988 det the,activity
R4836 T16988 T16986 nsubj activity,is
R4837 T16989 T16988 compound repression,activity
R4838 T16990 T16988 prep of,activity
R4839 T16991 T16992 compound mr,s
R4840 T16992 T16990 pobj s,of
R4841 T16993 T16992 punct -,s
R4842 T16994 T16986 neg not,is
R4843 T16995 T16986 prep due,is
R4844 T16996 T16995 prep to,due
R4845 T16997 T16998 det a,interaction
R4846 T16998 T16996 pobj interaction,to
R4847 T16999 T16998 amod homophilic,interaction
R4848 T17000 T16998 prep through,interaction
R4849 T17001 T17002 poss its,domain
R4850 T17002 T17000 pobj domain,through
R4851 T17003 T17002 compound SAM,domain
R4852 T17004 T16986 cc but,is
R4853 T17005 T16986 conj to,is
R4854 T17006 T17007 det the,region
R4855 T17007 T17005 pobj region,to
R4856 T17008 T17009 npadvmod C,terminal
R4857 T17009 T17007 amod terminal,region
R4858 T17010 T17009 punct -,terminal
R4859 T17011 T17012 punct (,463
R4860 T17012 T17007 parataxis 463,region
R4861 T17013 T17012 nmod amino,463
R4862 T17014 T17012 nmod acids,463
R4863 T17015 T17012 prep to,463
R4864 T17016 T17015 pobj 542,to
R4865 T17017 T17012 punct ),463
R4866 T17018 T16970 punct .,suggest
R9183 T32444 T32445 compound mr,s
R9184 T32445 T32447 nsubj s,functions
R9185 T32446 T32445 punct -,s
R9186 T32448 T32445 acl fused,s
R9187 T32449 T32448 prep to,fused
R9188 T32450 T32451 nmod GAL4,domain
R9189 T32451 T32449 pobj domain,to
R9190 T32452 T32453 npadvmod DNA,binding
R9191 T32453 T32451 amod binding,domain
R9192 T32454 T32447 prep as,functions
R9193 T32455 T32456 det a,repressor
R9194 T32456 T32454 pobj repressor,as
R9195 T32457 T32456 amod transcriptional,repressor
R9196 T32458 T32447 prep in,functions
R9197 T32459 T32460 compound HEK293T,cells
R9198 T32460 T32458 pobj cells,in
R9199 T32461 T32447 punct .,functions
R9200 T32463 T32464 punct (,A
R9201 T32464 T32465 meta A,drawing
R9202 T32466 T32464 punct ),A
R9203 T32467 T32465 amod Schematic,drawing
R9204 T32468 T32465 prep of,drawing
R9205 T32469 T32470 det the,constructs
R9206 T32470 T32468 pobj constructs,of
R9207 T32471 T32470 acl used,constructs
R9208 T32472 T32471 prep for,used
R9209 T32473 T32474 det the,assay
R9210 T32474 T32472 pobj assay,for
R9211 T32475 T32474 compound luciferase,assay
R9212 T32476 T32465 punct .,drawing
R9213 T32478 T32479 compound 5xGAL4,pGL3
R9214 T32479 T32481 compound pGL3,plasmid
R9215 T32480 T32479 punct -,pGL3
R9216 T32481 T32483 nsubjpass plasmid,co-transfected
R9217 T32482 T32481 compound reporter,plasmid
R9218 T32484 T32483 auxpass was,co-transfected
R9219 T32485 T32483 prep into,co-transfected
R9220 T32486 T32487 compound HEK293T,cells
R9221 T32487 T32485 pobj cells,into
R9222 T32488 T32487 prep with,cells
R9223 T32489 T32490 compound effector,plasmids
R9224 T32490 T32488 pobj plasmids,with
R9225 T32491 T32490 acl expressing,plasmids
R9226 T32492 T32493 amod various,mutants
R9227 T32493 T32491 dobj mutants,expressing
R9228 T32494 T32493 compound deletion,mutants
R9229 T32495 T32493 acl fused,mutants
R9230 T32496 T32495 prep to,fused
R9231 T32497 T32498 compound GAL4,DBD
R9232 T32498 T32496 pobj DBD,to
R9233 T32499 T32498 punct -,DBD
R9234 T32500 T32483 punct .,co-transfected
R9235 T32502 T32503 punct (,B
R9236 T32503 T32504 meta B,transfected
R9237 T32505 T32503 punct ),B
R9238 T32506 T32507 amod Various,amounts
R9239 T32507 T32504 nsubjpass amounts,transfected
R9240 T32508 T32507 prep of,amounts
R9241 T32509 T32510 nmod DBD,plasmids
R9242 T32510 T32508 pobj plasmids,of
R9243 T32511 T32509 punct ", ",DBD
R9244 T32512 T32513 compound DBD,mrs
R9245 T32513 T32509 conj mrs,DBD
R9246 T32514 T32513 punct -,mrs
R9247 T32515 T32513 punct ", ",mrs
R9248 T32516 T32517 compound DBD,N
R9249 T32517 T32513 conj N,mrs
R9250 T32518 T32517 punct -,N
R9251 T32519 T32517 cc or,N
R9252 T32520 T32521 compound DBD,C
R9253 T32521 T32517 conj C,N
R9254 T32522 T32521 punct -,C
R9255 T32523 T32504 auxpass were,transfected
R9256 T32524 T32504 prep with,transfected
R9257 T32525 T32526 nummod 0.1,μg
R9258 T32526 T32524 pobj μg,with
R9259 T32527 T32526 prep of,μg
R9260 T32528 T32529 compound 5xGAL4,pGL3
R9261 T32529 T32531 compound pGL3,plasmid
R9262 T32530 T32529 punct -,pGL3
R9263 T32531 T32527 pobj plasmid,of
R9264 T32532 T32531 compound reporter,plasmid
R9265 T32533 T32504 punct .,transfected
R9266 T32535 T32536 det The,activity
R9267 T32536 T32538 nsubjpass activity,designated
R9268 T32537 T32536 compound reporter,activity
R9269 T32539 T32536 prep in,activity
R9270 T32540 T32541 det the,presence
R9271 T32541 T32539 pobj presence,in
R9272 T32542 T32541 prep of,presence
R9273 T32543 T32544 det the,vector
R9274 T32544 T32542 pobj vector,of
R9275 T32545 T32544 compound pcDNA3,vector
R9276 T32546 T32547 punct (,pcDNA3
R9277 T32547 T32536 parataxis pcDNA3,activity
R9278 T32548 T32547 punct ),pcDNA3
R9279 T32549 T32538 auxpass was,designated
R9280 T32550 T32538 prep as,designated
R9281 T32551 T32550 pobj 1,as
R9282 T32552 T32538 punct .,designated
R9283 T32554 T32555 compound Error,bars
R9284 T32555 T32556 nsubj bars,represent
R9285 T32557 T32558 amod standard,error
R9286 T32558 T32556 dobj error,represent
R9287 T32559 T32558 prep of,error
R9288 T32560 T32559 pobj mean,of
R9289 T32561 T32556 punct .,represent
R9290 T32563 T32564 punct (,C
R9291 T32564 T32565 meta C,co-transfected
R9292 T32566 T32564 punct ),C
R9293 T32567 T32568 compound DBD,W404A
R9294 T32568 T32565 nsubjpass W404A,co-transfected
R9295 T32569 T32568 punct -,W404A
R9296 T32570 T32568 cc and,W404A
R9297 T32571 T32572 compound DBD,G453A
R9298 T32572 T32568 conj G453A,W404A
R9299 T32573 T32572 punct -,G453A
R9300 T32574 T32565 auxpass were,co-transfected
R9301 T32575 T32565 prep into,co-transfected
R9302 T32576 T32577 compound HEK293T,cells
R9303 T32577 T32575 pobj cells,into
R9304 T32578 T32565 prep with,co-transfected
R9305 T32579 T32580 compound 5xGAL4,pGL3
R9306 T32580 T32582 compound pGL3,plasmid
R9307 T32581 T32580 punct -,pGL3
R9308 T32582 T32578 pobj plasmid,with
R9309 T32583 T32582 compound reporter,plasmid
R9310 T32584 T32565 punct .,co-transfected
R9311 T32586 T32587 compound Fold,repression
R9312 T32587 T32588 nsubjpass repression,calculated
R9313 T32589 T32588 auxpass was,calculated
R9314 T32590 T32588 prep as,calculated
R9315 T32591 T32592 det the,decrease
R9316 T32592 T32590 pobj decrease,as
R9317 T32593 T32592 compound fold,decrease
R9318 T32594 T32592 prep in,decrease
R9319 T32595 T32596 compound luciferase,activity
R9320 T32596 T32594 pobj activity,in
R9321 T32597 T32592 prep compared,decrease
R9322 T32598 T32597 prep with,compared
R9323 T32599 T32600 compound DBD,mrs
R9324 T32600 T32598 pobj mrs,with
R9325 T32601 T32600 punct -,mrs
R9326 T32602 T32588 punct .,calculated
R9327 T32604 T32605 compound Error,bars
R9328 T32605 T32606 nsubj bars,represent
R9329 T32607 T32608 amod standard,deviation
R9330 T32608 T32606 dobj deviation,represent
R9331 T32609 T32606 punct .,represent
R9332 T32611 T32612 punct (,D
R9333 T32612 T32613 meta D,transfected
R9334 T32614 T32612 punct ),D
R9335 T32615 T32616 amod Various,amounts
R9336 T32616 T32613 nsubjpass amounts,transfected
R9337 T32617 T32616 prep of,amounts
R9338 T32618 T32619 compound DBD,tail
R9339 T32619 T32617 pobj tail,of
R9340 T32620 T32619 punct -,tail
R9341 T32621 T32619 cc or,tail
R9342 T32622 T32623 compound DBD,SAM
R9343 T32623 T32619 conj SAM,tail
R9344 T32624 T32623 punct -,SAM
R9345 T32625 T32613 auxpass were,transfected
R9346 T32626 T32613 prep with,transfected
R9347 T32627 T32628 compound 5xGAL4,pGL3
R9348 T32628 T32630 compound pGL3,plasmid
R9349 T32629 T32628 punct -,pGL3
R9350 T32630 T32626 pobj plasmid,with
R9351 T32631 T32630 compound reporter,plasmid
R9352 T32632 T32613 punct .,transfected
R9353 T32634 T32635 compound Error,bars
R9354 T32635 T32636 nsubj bars,represent
R9355 T32637 T32638 amod standard,error
R9356 T32638 T32636 dobj error,represent
R9357 T32639 T32638 prep of,error
R9358 T32640 T32639 pobj mean,of
R9359 T32641 T32636 punct .,represent
R9360 T32643 T32644 punct (,E
R9361 T32644 T32645 meta E,co-transfected
R9362 T32646 T32644 punct ),E
R9363 T32647 T32645 nsubjpass pcDNA3,co-transfected
R9364 T32648 T32647 cc or,pcDNA3
R9365 T32649 T32650 compound DBD,mrs
R9366 T32650 T32647 conj mrs,pcDNA3
R9367 T32651 T32650 punct -,mrs
R9368 T32652 T32653 punct (,μg
R9369 T32653 T32650 parataxis μg,mrs
R9370 T32654 T32653 nummod 5,μg
R9371 T32655 T32653 punct ),μg
R9372 T32656 T32645 auxpass was,co-transfected
R9373 T32657 T32645 prep into,co-transfected
R9374 T32658 T32659 compound Y79,cells
R9375 T32659 T32657 pobj cells,into
R9376 T32660 T32659 compound retinoblastoma,cells
R9377 T32661 T32645 prep with,co-transfected
R9378 T32662 T32663 nummod 0.5,μg
R9379 T32663 T32661 pobj μg,with
R9380 T32664 T32663 prep of,μg
R9381 T32665 T32666 compound 5xGAL4,pGL3
R9382 T32666 T32668 compound pGL3,plasmid
R9383 T32667 T32666 punct -,pGL3
R9384 T32668 T32664 pobj plasmid,of
R9385 T32669 T32668 compound reporter,plasmid
R9386 T32670 T32645 punct .,co-transfected
R9387 T32672 T32673 det The,activity
R9388 T32673 T32675 nsubjpass activity,designated
R9389 T32674 T32673 compound reporter,activity
R9390 T32676 T32673 prep in,activity
R9391 T32677 T32678 det the,presence
R9392 T32678 T32676 pobj presence,in
R9393 T32679 T32678 prep of,presence
R9394 T32680 T32679 pobj pcDNA3,of
R9395 T32681 T32675 auxpass was,designated
R9396 T32682 T32675 prep as,designated
R9397 T32683 T32682 pobj 1,as
R9398 T32684 T32675 punct .,designated
R9399 T32686 T32687 compound Error,bars
R9400 T32687 T32688 nsubj bars,represent
R9401 T32689 T32690 amod standard,deviation
R9402 T32690 T32688 dobj deviation,represent
R9403 T32691 T32688 punct .,represent
R9404 T32693 T32694 nsubj Asterisk,marks
R9405 T32695 T32696 advmod statistically,significant
R9406 T32696 T32697 amod significant,difference
R9407 T32697 T32694 dobj difference,marks
R9408 T32698 T32699 punct (,test
R9409 T32699 T32694 parataxis test,marks
R9410 T32700 T32699 poss Student,test
R9411 T32701 T32700 case 's,Student
R9412 T32702 T32699 compound t,test
R9413 T32703 T32699 punct : ,test
R9414 T32704 T32705 nsubj p,0.03
R9415 T32705 T32699 ccomp 0.03,test
R9416 T32706 T32705 punct <,0.03
R9417 T32707 T32699 punct ),test
R9418 T32708 T32694 punct .,marks
R9419 T32710 T32711 punct (,F
R9420 T32711 T32712 meta F,Alignment
R9421 T32713 T32711 punct ),F
R9422 T32714 T32712 prep of,Alignment
R9423 T32715 T32716 det the,regions
R9424 T32716 T32714 pobj regions,of
R9425 T32717 T32718 npadvmod C,terminal
R9426 T32718 T32716 amod terminal,regions
R9427 T32719 T32718 punct -,terminal
R9428 T32720 T32716 prep for,regions
R9429 T32721 T32722 nmod mouse,proteins
R9430 T32722 T32720 pobj proteins,for
R9431 T32723 T32721 punct ", ",mouse
R9432 T32724 T32721 conj rat,mouse
R9433 T32725 T32724 punct ", ",rat
R9434 T32726 T32724 amod human,rat
R9435 T32727 T32724 punct ", ",rat
R9436 T32728 T32724 conj chick,rat
R9437 T32729 T32728 cc and,chick
R9438 T32730 T32728 conj zebrafish,chick
R9439 T32731 T32732 compound mr,s
R9440 T32732 T32722 compound s,proteins
R9441 T32733 T32732 punct -,s
R9442 T32734 T32712 punct .,Alignment
R9443 T32736 T32737 amod Conserved,residues
R9444 T32737 T32740 nsubjpass residues,shown
R9445 T32738 T32739 compound amino,acid
R9446 T32739 T32737 compound acid,residues
R9447 T32741 T32740 auxpass are,shown
R9448 T32742 T32740 prep with,shown
R9449 T32743 T32744 det a,shadow
R9450 T32744 T32742 pobj shadow,with
R9451 T32745 T32744 amod dark,shadow
R9452 T32746 T32740 punct .,shown