PMC:1435744 / 21507-22520 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T12999 0-2 TO denotes To
T12998 0-246 sentence denotes To confirm self-association of the mr-s protein in mammalian cells, we next performed co-immunoprecipitation studies in HEK293T cells by co-transfection of HA-tagged full-length/truncated mr-s and Flag-tagged full-length/truncated mr-s (Fig. 5A).
T13000 3-10 VB denotes confirm
T13002 11-15 NN denotes self
T13004 15-16 HYPH denotes -
T13003 16-27 NN denotes association
T13005 28-30 IN denotes of
T13006 31-34 DT denotes the
T13008 35-37 NN denotes mr
T13010 37-38 HYPH denotes -
T13009 38-39 NN denotes s
T13007 40-47 NN denotes protein
T13011 48-50 IN denotes in
T13012 51-60 JJ denotes mammalian
T13013 61-66 NNS denotes cells
T13014 66-68 , denotes ,
T13015 68-70 PRP denotes we
T13016 71-75 RB denotes next
T13001 76-85 VBD denotes performed
T13017 86-108 NN denotes co-immunoprecipitation
T13018 109-116 NNS denotes studies
T13019 117-119 IN denotes in
T13020 120-127 NN denotes HEK293T
T13021 128-133 NNS denotes cells
T13022 134-136 IN denotes by
T13023 137-152 NN denotes co-transfection
T13024 153-155 IN denotes of
T13025 156-158 NN denotes HA
T13027 158-159 HYPH denotes -
T13026 159-165 VBN denotes tagged
T13029 166-170 JJ denotes full
T13031 170-171 HYPH denotes -
T13030 171-177 NN denotes length
T13033 177-178 HYPH denotes /
T13032 178-187 VBN denotes truncated
T13034 188-190 NN denotes mr
T13035 190-191 HYPH denotes -
T13028 191-192 NN denotes s
T13036 193-196 CC denotes and
T13037 197-201 NN denotes Flag
T13039 201-202 HYPH denotes -
T13038 202-208 VBN denotes tagged
T13041 209-213 JJ denotes full
T13043 213-214 HYPH denotes -
T13042 214-220 NN denotes length
T13045 220-221 HYPH denotes /
T13044 221-230 VBN denotes truncated
T13046 231-233 NN denotes mr
T13047 233-234 HYPH denotes -
T13040 234-235 NN denotes s
T13048 236-237 -LRB- denotes (
T13050 237-241 NN denotes Fig.
T13049 242-244 NN denotes 5A
T13051 244-245 -RRB- denotes )
T13052 245-246 . denotes .
T13053 246-333 sentence denotes As a negative control, we constructed Flag-tagged Sonic hedgehog (Shh) (lane 2 and 7).
T13054 247-249 IN denotes As
T13056 250-251 DT denotes a
T13058 252-260 JJ denotes negative
T13057 261-268 NN denotes control
T13059 268-270 , denotes ,
T13060 270-272 PRP denotes we
T13055 273-284 VBD denotes constructed
T13061 285-289 NN denotes Flag
T13063 289-290 HYPH denotes -
T13062 290-296 VBN denotes tagged
T13065 297-302 JJ denotes Sonic
T13064 303-311 NN denotes hedgehog
T13066 312-313 -LRB- denotes (
T13067 313-316 NN denotes Shh
T13068 316-317 -RRB- denotes )
T13069 318-319 -LRB- denotes (
T13071 319-323 NN denotes lane
T13070 324-325 CD denotes 2
T13072 326-329 CC denotes and
T13073 330-331 CD denotes 7
T13074 331-332 -RRB- denotes )
T13075 332-333 . denotes .
T13076 333-624 sentence denotes In accordance with the result of the yeast two-hybrid GAL4 assay, HA-tagged full-length mr-s (full-HA) was co-immunoprecipitated with Flag-tagged full-length mr-s (Flag-mrs) and the Flag-tagged C-terminus portion containing the SAM domain (Flag-SAM), respectively (Fig 5B, lane3 and lane5).
T13077 334-336 IN denotes In
T13079 337-347 NN denotes accordance
T13080 348-352 IN denotes with
T13081 353-356 DT denotes the
T13082 357-363 NN denotes result
T13083 364-366 IN denotes of
T13084 367-370 DT denotes the
T13086 371-376 NN denotes yeast
T13087 377-380 CD denotes two
T13089 380-381 HYPH denotes -
T13088 381-387 NN denotes hybrid
T13090 388-392 NN denotes GAL4
T13085 393-398 NN denotes assay
T13091 398-400 , denotes ,
T13092 400-402 NN denotes HA
T13094 402-403 HYPH denotes -
T13093 403-409 VBN denotes tagged
T13096 410-414 JJ denotes full
T13098 414-415 HYPH denotes -
T13097 415-421 NN denotes length
T13099 422-424 NN denotes mr
T13100 424-425 HYPH denotes -
T13095 425-426 NN denotes s
T13101 427-428 -LRB- denotes (
T13102 428-432 JJ denotes full
T13104 432-433 HYPH denotes -
T13103 433-435 NN denotes HA
T13105 435-436 -RRB- denotes )
T13106 437-440 VBD denotes was
T13078 441-462 VBN denotes co-immunoprecipitated
T13107 463-467 IN denotes with
T13108 468-472 NN denotes Flag
T13110 472-473 HYPH denotes -
T13109 473-479 VBN denotes tagged
T13112 480-484 JJ denotes full
T13114 484-485 HYPH denotes -
T13113 485-491 NN denotes length
T13115 492-494 NN denotes mr
T13116 494-495 HYPH denotes -
T13111 495-496 NN denotes s
T13117 497-498 -LRB- denotes (
T13118 498-502 NN denotes Flag
T13120 502-503 HYPH denotes -
T13119 503-506 NN denotes mrs
T13121 506-507 -RRB- denotes )
T13122 508-511 CC denotes and
T13123 512-515 DT denotes the
T13125 516-520 NN denotes Flag
T13127 520-521 HYPH denotes -
T13126 521-527 VBN denotes tagged
T13128 528-529 NN denotes C
T13130 529-530 HYPH denotes -
T13129 530-538 NN denotes terminus
T13124 539-546 NN denotes portion
T13131 547-557 VBG denotes containing
T13132 558-561 DT denotes the
T13134 562-565 NN denotes SAM
T13133 566-572 NN denotes domain
T13135 573-574 -LRB- denotes (
T13136 574-578 NN denotes Flag
T13138 578-579 HYPH denotes -
T13137 579-582 NN denotes SAM
T13139 582-583 -RRB- denotes )
T13140 583-585 , denotes ,
T13141 585-597 RB denotes respectively
T13142 598-599 -LRB- denotes (
T13144 599-602 NN denotes Fig
T13145 603-605 NN denotes 5B
T13146 605-607 , denotes ,
T13143 607-612 NN denotes lane3
T13147 613-616 CC denotes and
T13148 617-622 NN denotes lane5
T13149 622-623 -RRB- denotes )
T13150 623-624 . denotes .
T13151 624-776 sentence denotes We also found a weak co-immunoprecipitation band in co-transfection of full-HA and Flag-tagged N-terminus portion of mr-s (Flag-ΔSAM, Fig. 5B, lane 4).
T13152 625-627 PRP denotes We
T13154 628-632 RB denotes also
T13153 633-638 VBD denotes found
T13155 639-640 DT denotes a
T13157 641-645 JJ denotes weak
T13158 646-668 NN denotes co-immunoprecipitation
T13156 669-673 NN denotes band
T13159 674-676 IN denotes in
T13160 677-692 NN denotes co-transfection
T13161 693-695 IN denotes of
T13162 696-700 JJ denotes full
T13164 700-701 HYPH denotes -
T13163 701-703 NN denotes HA
T13165 704-707 CC denotes and
T13166 708-712 NN denotes Flag
T13168 712-713 HYPH denotes -
T13167 713-719 VBN denotes tagged
T13170 720-721 NN denotes N
T13172 721-722 HYPH denotes -
T13171 722-730 NN denotes terminus
T13169 731-738 NN denotes portion
T13173 739-741 IN denotes of
T13174 742-744 NN denotes mr
T13176 744-745 HYPH denotes -
T13175 745-746 NN denotes s
T13177 747-748 -LRB- denotes (
T13178 748-752 NN denotes Flag
T13180 752-753 HYPH denotes -
T13179 753-757 NN denotes ΔSAM
T13181 757-759 , denotes ,
T13183 759-763 NN denotes Fig.
T13184 764-766 NN denotes 5B
T13185 766-768 , denotes ,
T13182 768-772 NN denotes lane
T13186 773-774 CD denotes 4
T13187 774-775 -RRB- denotes )
T13188 775-776 . denotes .
T13189 776-1013 sentence denotes When ΔSAM-HA and Flag-tagged deletion mutants were co-transfected, ΔSAM-HA was co-immunoprecipitated with Flag-mrs and Flag-ΔSAM (Fig. 5B, lane 8 and lane 9), while ΔSAM-HA was not co-immunoprecipitated with Flag-SAM (Fig. 5B, lane 10).
T13190 777-781 WRB denotes When
T13192 782-786 NN denotes ΔSAM
T13194 786-787 HYPH denotes -
T13193 787-789 NN denotes HA
T13195 790-793 CC denotes and
T13196 794-798 NN denotes Flag
T13198 798-799 HYPH denotes -
T13197 799-805 VBN denotes tagged
T13200 806-814 NN denotes deletion
T13199 815-822 NNS denotes mutants
T13201 823-827 VBD denotes were
T13191 828-842 VBN denotes co-transfected
T13203 842-844 , denotes ,
T13204 844-848 NN denotes ΔSAM
T13206 848-849 HYPH denotes -
T13205 849-851 NN denotes HA
T13207 852-855 VBD denotes was
T13202 856-877 VBN denotes co-immunoprecipitated
T13208 878-882 IN denotes with
T13209 883-887 NN denotes Flag
T13211 887-888 HYPH denotes -
T13210 888-891 NN denotes mrs
T13212 892-895 CC denotes and
T13213 896-900 NN denotes Flag
T13215 900-901 HYPH denotes -
T13214 901-905 NN denotes ΔSAM
T13216 906-907 -LRB- denotes (
T13218 907-911 NN denotes Fig.
T13217 912-914 NN denotes 5B
T13219 914-916 , denotes ,
T13220 916-920 NN denotes lane
T13221 921-922 CD denotes 8
T13222 923-926 CC denotes and
T13223 927-931 NN denotes lane
T13224 932-933 CD denotes 9
T13225 933-934 -RRB- denotes )
T13226 934-936 , denotes ,
T13227 936-941 IN denotes while
T13229 942-946 NN denotes ΔSAM
T13231 946-947 HYPH denotes -
T13230 947-949 NN denotes HA
T13232 950-953 VBD denotes was
T13233 954-957 RB denotes not
T13228 958-979 VBN denotes co-immunoprecipitated
T13234 980-984 IN denotes with
T13235 985-989 NN denotes Flag
T13237 989-990 HYPH denotes -
T13236 990-993 NN denotes SAM
T13238 994-995 -LRB- denotes (
T13240 995-999 NN denotes Fig.
T13239 1000-1002 NN denotes 5B
T13241 1002-1004 , denotes ,
T13242 1004-1008 NN denotes lane
T13243 1009-1011 CD denotes 10
T13244 1011-1012 -RRB- denotes )
T13245 1012-1013 . denotes .
R3367 T12999 T13000 aux To,confirm
R3368 T13000 T13001 advcl confirm,performed
R3369 T13002 T13003 compound self,association
R3370 T13003 T13000 dobj association,confirm
R3371 T13004 T13003 punct -,association
R3372 T13005 T13003 prep of,association
R3373 T13006 T13007 det the,protein
R3374 T13007 T13005 pobj protein,of
R3375 T13008 T13009 compound mr,s
R3376 T13009 T13007 compound s,protein
R3377 T13010 T13009 punct -,s
R3378 T13011 T13000 prep in,confirm
R3379 T13012 T13013 amod mammalian,cells
R3380 T13013 T13011 pobj cells,in
R3381 T13014 T13001 punct ", ",performed
R3382 T13015 T13001 nsubj we,performed
R3383 T13016 T13001 advmod next,performed
R3384 T13017 T13018 compound co-immunoprecipitation,studies
R3385 T13018 T13001 dobj studies,performed
R3386 T13019 T13001 prep in,performed
R3387 T13020 T13021 compound HEK293T,cells
R3388 T13021 T13019 pobj cells,in
R3389 T13022 T13001 prep by,performed
R3390 T13023 T13022 pobj co-transfection,by
R3391 T13024 T13023 prep of,co-transfection
R3392 T13025 T13026 npadvmod HA,tagged
R3393 T13026 T13028 amod tagged,s
R3394 T13027 T13026 punct -,tagged
R3395 T13028 T13024 pobj s,of
R3396 T13029 T13030 amod full,length
R3397 T13030 T13032 dep length,truncated
R3398 T13031 T13030 punct -,length
R3399 T13032 T13028 amod truncated,s
R3400 T13033 T13032 punct /,truncated
R3401 T13034 T13028 compound mr,s
R3402 T13035 T13028 punct -,s
R3403 T13036 T13028 cc and,s
R3404 T13037 T13038 npadvmod Flag,tagged
R3405 T13038 T13040 amod tagged,s
R3406 T13039 T13038 punct -,tagged
R3407 T13040 T13028 conj s,s
R3408 T13041 T13042 amod full,length
R3409 T13042 T13044 dep length,truncated
R3410 T13043 T13042 punct -,length
R3411 T13044 T13040 amod truncated,s
R3412 T13045 T13044 punct /,truncated
R3413 T13046 T13040 compound mr,s
R3414 T13047 T13040 punct -,s
R3415 T13048 T13049 punct (,5A
R3416 T13049 T13001 parataxis 5A,performed
R3417 T13050 T13049 compound Fig.,5A
R3418 T13051 T13049 punct ),5A
R3419 T13052 T13001 punct .,performed
R3420 T13054 T13055 prep As,constructed
R3421 T13056 T13057 det a,control
R3422 T13057 T13054 pobj control,As
R3423 T13058 T13057 amod negative,control
R3424 T13059 T13055 punct ", ",constructed
R3425 T13060 T13055 nsubj we,constructed
R3426 T13061 T13062 npadvmod Flag,tagged
R3427 T13062 T13064 amod tagged,hedgehog
R3428 T13063 T13062 punct -,tagged
R3429 T13064 T13055 dobj hedgehog,constructed
R3430 T13065 T13064 amod Sonic,hedgehog
R3431 T13066 T13064 punct (,hedgehog
R3432 T13067 T13064 appos Shh,hedgehog
R3433 T13068 T13055 punct ),constructed
R3434 T13069 T13070 punct (,2
R3435 T13070 T13055 parataxis 2,constructed
R3436 T13071 T13070 nmod lane,2
R3437 T13072 T13070 cc and,2
R3438 T13073 T13070 conj 7,2
R3439 T13074 T13070 punct ),2
R3440 T13075 T13055 punct .,constructed
R3441 T13077 T13078 prep In,co-immunoprecipitated
R3442 T13079 T13077 pobj accordance,In
R3443 T13080 T13079 prep with,accordance
R3444 T13081 T13082 det the,result
R3445 T13082 T13080 pobj result,with
R3446 T13083 T13082 prep of,result
R3447 T13084 T13085 det the,assay
R3448 T13085 T13083 pobj assay,of
R3449 T13086 T13085 nmod yeast,assay
R3450 T13087 T13088 nummod two,hybrid
R3451 T13088 T13085 compound hybrid,assay
R3452 T13089 T13088 punct -,hybrid
R3453 T13090 T13085 compound GAL4,assay
R3454 T13091 T13078 punct ", ",co-immunoprecipitated
R3455 T13092 T13093 npadvmod HA,tagged
R3456 T13093 T13095 amod tagged,s
R3457 T13094 T13093 punct -,tagged
R3458 T13095 T13078 nsubjpass s,co-immunoprecipitated
R3459 T13096 T13097 amod full,length
R3460 T13097 T13095 compound length,s
R3461 T13098 T13097 punct -,length
R3462 T13099 T13095 compound mr,s
R3463 T13100 T13095 punct -,s
R3464 T13101 T13095 punct (,s
R3465 T13102 T13103 amod full,HA
R3466 T13103 T13095 appos HA,s
R3467 T13104 T13103 punct -,HA
R3468 T13105 T13078 punct ),co-immunoprecipitated
R3469 T13106 T13078 auxpass was,co-immunoprecipitated
R3470 T13107 T13078 prep with,co-immunoprecipitated
R3471 T13108 T13109 npadvmod Flag,tagged
R3472 T13109 T13111 amod tagged,s
R3473 T13110 T13109 punct -,tagged
R3474 T13111 T13107 pobj s,with
R3475 T13112 T13113 amod full,length
R3476 T13113 T13111 compound length,s
R3477 T13114 T13113 punct -,length
R3478 T13115 T13111 compound mr,s
R3479 T13116 T13111 punct -,s
R3480 T13117 T13111 punct (,s
R3481 T13118 T13119 compound Flag,mrs
R3482 T13119 T13111 appos mrs,s
R3483 T13120 T13119 punct -,mrs
R3484 T13121 T13111 punct ),s
R3485 T13122 T13111 cc and,s
R3486 T13123 T13124 det the,portion
R3487 T13124 T13111 conj portion,s
R3488 T13125 T13126 npadvmod Flag,tagged
R3489 T13126 T13124 amod tagged,portion
R3490 T13127 T13126 punct -,tagged
R3491 T13128 T13129 compound C,terminus
R3492 T13129 T13124 compound terminus,portion
R3493 T13130 T13129 punct -,terminus
R3494 T13131 T13124 acl containing,portion
R3495 T13132 T13133 det the,domain
R3496 T13133 T13131 dobj domain,containing
R3497 T13134 T13133 compound SAM,domain
R3498 T13135 T13124 punct (,portion
R3499 T13136 T13137 compound Flag,SAM
R3500 T13137 T13124 appos SAM,portion
R3501 T13138 T13137 punct -,SAM
R3502 T13139 T13078 punct ),co-immunoprecipitated
R3503 T13140 T13078 punct ", ",co-immunoprecipitated
R3504 T13141 T13078 advmod respectively,co-immunoprecipitated
R3505 T13142 T13143 punct (,lane3
R3506 T13143 T13078 parataxis lane3,co-immunoprecipitated
R3507 T13144 T13145 compound Fig,5B
R3508 T13145 T13143 dep 5B,lane3
R3509 T13146 T13143 punct ", ",lane3
R3510 T13147 T13143 cc and,lane3
R3511 T13148 T13143 conj lane5,lane3
R3512 T13149 T13143 punct ),lane3
R3513 T13150 T13078 punct .,co-immunoprecipitated
R3514 T13152 T13153 nsubj We,found
R3515 T13154 T13153 advmod also,found
R3516 T13155 T13156 det a,band
R3517 T13156 T13153 dobj band,found
R3518 T13157 T13156 amod weak,band
R3519 T13158 T13156 compound co-immunoprecipitation,band
R3520 T13159 T13153 prep in,found
R3521 T13160 T13159 pobj co-transfection,in
R3522 T13161 T13160 prep of,co-transfection
R3523 T13162 T13163 amod full,HA
R3524 T13163 T13161 pobj HA,of
R3525 T13164 T13163 punct -,HA
R3526 T13165 T13163 cc and,HA
R3527 T13166 T13167 npadvmod Flag,tagged
R3528 T13167 T13169 amod tagged,portion
R3529 T13236 T13234 pobj SAM,with
R3530 T13168 T13167 punct -,tagged
R3531 T13237 T13236 punct -,SAM
R3532 T13238 T13239 punct (,5B
R3533 T13169 T13163 conj portion,HA
R3534 T13239 T13228 parataxis 5B,co-immunoprecipitated
R3535 T13240 T13239 compound Fig.,5B
R3536 T13170 T13171 compound N,terminus
R3537 T13241 T13239 punct ", ",5B
R3538 T13242 T13239 appos lane,5B
R3539 T13243 T13242 nummod 10,lane
R3540 T13171 T13169 compound terminus,portion
R3541 T13244 T13239 punct ),5B
R3542 T13245 T13202 punct .,co-immunoprecipitated
R3543 T13172 T13171 punct -,terminus
R3544 T13173 T13169 prep of,portion
R3545 T13174 T13175 compound mr,s
R3546 T13175 T13173 pobj s,of
R3547 T13176 T13175 punct -,s
R3548 T13177 T13169 punct (,portion
R3550 T13178 T13179 compound Flag,ΔSAM
R3552 T13179 T13169 appos ΔSAM,portion
R3555 T13180 T13179 punct -,ΔSAM
R3559 T13181 T13182 punct ", ",lane
R3562 T13182 T13153 parataxis lane,found
R3566 T13183 T13184 compound Fig.,5B
R3570 T13184 T13182 dep 5B,lane
R3573 T13185 T13182 punct ", ",lane
R3577 T13186 T13182 nummod 4,lane
R3581 T13187 T13182 punct ),lane
R3585 T13188 T13153 punct .,found
R3589 T13190 T13191 advmod When,co-transfected
R3590 T13191 T13202 advcl co-transfected,co-immunoprecipitated
R3592 T13192 T13193 compound ΔSAM,HA
R3598 T13193 T13191 nsubjpass HA,co-transfected
R3600 T13194 T13193 punct -,HA
R3601 T13195 T13193 cc and,HA
R3603 T13196 T13197 npadvmod Flag,tagged
R3605 T13197 T13199 amod tagged,mutants
R3608 T13198 T13197 punct -,tagged
R3611 T13199 T13193 conj mutants,HA
R3615 T13200 T13199 compound deletion,mutants
R3619 T13201 T13191 auxpass were,co-transfected
R3623 T13203 T13202 punct ", ",co-immunoprecipitated
R3628 T13204 T13205 compound ΔSAM,HA
R3631 T13205 T13202 nsubjpass HA,co-immunoprecipitated
R3634 T13206 T13205 punct -,HA
R3638 T13207 T13202 auxpass was,co-immunoprecipitated
R3642 T13208 T13202 prep with,co-immunoprecipitated
R3646 T13209 T13210 compound Flag,mrs
R3652 T13210 T13208 pobj mrs,with
R3657 T13211 T13210 punct -,mrs
R3661 T13212 T13210 cc and,mrs
R3664 T13213 T13214 compound Flag,ΔSAM
R3668 T13214 T13210 conj ΔSAM,mrs
R3671 T13215 T13214 punct -,ΔSAM
R3673 T13216 T13217 punct (,5B
R3677 T13217 T13202 parataxis 5B,co-immunoprecipitated
R3681 T13218 T13217 compound Fig.,5B
R3685 T13219 T13217 punct ", ",5B
R3688 T13220 T13217 appos lane,5B
R3691 T13221 T13220 nummod 8,lane
R3695 T13222 T13220 cc and,lane
R3699 T13223 T13220 conj lane,lane
R3703 T13224 T13223 nummod 9,lane
R3705 T13225 T13217 punct ),5B
R3709 T13226 T13202 punct ", ",co-immunoprecipitated
R3712 T13227 T13228 mark while,co-immunoprecipitated
R3716 T13228 T13202 advcl co-immunoprecipitated,co-immunoprecipitated
R3720 T13229 T13230 compound ΔSAM,HA
R3724 T13230 T13228 nsubjpass HA,co-immunoprecipitated
R3728 T13231 T13230 punct -,HA
R3732 T13232 T13228 auxpass was,co-immunoprecipitated
R3734 T13233 T13228 neg not,co-immunoprecipitated
R3735 T13234 T13228 prep with,co-immunoprecipitated
R3739 T13235 T13236 compound Flag,SAM