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PMC:1420314 / 19560-20441 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T13011 0-7 NNP denotes Western
T13012 8-12 NN denotes blot
T13013 13-21 NN denotes analysis
T13014 21-109 sentence denotes The absence of ADAM11 protein in the (-/-) mice was confirmed by Western blot analysis.
T13015 22-25 DT denotes The
T13016 26-33 NN denotes absence
T13018 34-36 IN denotes of
T13019 37-43 NN denotes ADAM11
T13020 44-51 NN denotes protein
T13021 52-54 IN denotes in
T13022 55-58 DT denotes the
T13024 59-60 -LRB- denotes (
T13025 60-61 SYM denotes -
T13027 61-62 HYPH denotes /
T13026 62-63 SYM denotes -
T13028 63-64 -RRB- denotes )
T13023 65-69 NNS denotes mice
T13029 70-73 VBD denotes was
T13017 74-83 VBN denotes confirmed
T13030 84-86 IN denotes by
T13031 87-94 NNP denotes Western
T13032 95-99 NN denotes blot
T13033 100-108 NN denotes analysis
T13034 108-109 . denotes .
T13035 109-329 sentence denotes To be brief, the cerebellum was isolated from a six month-old mouse of each genotype and homogenised in cell lysis buffer (50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1% NP-40, protease inhibitor cocktail [Roche Diagnostics]).
T13036 110-112 TO denotes To
T13037 113-115 VB denotes be
T13039 116-121 JJ denotes brief
T13040 121-123 , denotes ,
T13041 123-126 DT denotes the
T13042 127-137 NN denotes cerebellum
T13043 138-141 VBD denotes was
T13038 142-150 VBN denotes isolated
T13044 151-155 IN denotes from
T13045 156-157 DT denotes a
T13047 158-161 CD denotes six
T13048 162-167 NN denotes month
T13050 167-168 HYPH denotes -
T13049 168-171 JJ denotes old
T13046 172-177 NN denotes mouse
T13051 178-180 IN denotes of
T13052 181-185 DT denotes each
T13053 186-194 NN denotes genotype
T13054 195-198 CC denotes and
T13055 199-210 VBN denotes homogenised
T13056 211-213 IN denotes in
T13057 214-218 NN denotes cell
T13058 219-224 NN denotes lysis
T13059 225-231 NN denotes buffer
T13060 232-233 -LRB- denotes (
T13062 233-235 CD denotes 50
T13063 236-238 NN denotes mM
T13065 239-243 NN denotes Tris
T13066 243-244 HYPH denotes -
T13064 244-247 NN denotes HCl
T13067 247-249 , denotes ,
T13068 249-251 NN denotes pH
T13069 252-255 CD denotes 7.5
T13070 255-257 , denotes ,
T13071 257-260 CD denotes 100
T13072 261-263 NN denotes mM
T13073 264-268 NN denotes NaCl
T13074 268-270 , denotes ,
T13075 270-271 CD denotes 1
T13076 271-272 NN denotes %
T13077 273-275 NN denotes NP
T13078 275-276 HYPH denotes -
T13079 276-278 CD denotes 40
T13080 278-280 , denotes ,
T13081 280-288 NN denotes protease
T13082 289-298 NN denotes inhibitor
T13061 299-307 NN denotes cocktail
T13083 308-309 -LRB- denotes [
T13085 309-314 NNP denotes Roche
T13084 315-326 NNP denotes Diagnostics
T13086 326-327 -RRB- denotes ]
T13087 327-328 -RRB- denotes )
T13088 328-329 . denotes .
T13089 329-526 sentence denotes After removal of cell debris by centrifugation, the glycosylated proteins in the supernatant were concentrated by the affinity chromatography using Con A Sepharose 4B (Amersham Biosciences Corp.).
T13090 330-335 IN denotes After
T13092 336-343 NN denotes removal
T13093 344-346 IN denotes of
T13094 347-351 NN denotes cell
T13095 352-358 NN denotes debris
T13096 359-361 IN denotes by
T13097 362-376 NN denotes centrifugation
T13098 376-378 , denotes ,
T13099 378-381 DT denotes the
T13101 382-394 VBN denotes glycosylated
T13100 395-403 NN denotes proteins
T13102 404-406 IN denotes in
T13103 407-410 DT denotes the
T13104 411-422 NN denotes supernatant
T13105 423-427 VBD denotes were
T13091 428-440 VBN denotes concentrated
T13106 441-443 IN denotes by
T13107 444-447 DT denotes the
T13109 448-456 NN denotes affinity
T13108 457-471 NN denotes chromatography
T13110 472-477 VBG denotes using
T13111 478-481 NN denotes Con
T13113 482-483 NN denotes A
T13114 484-493 NN denotes Sepharose
T13112 494-496 NN denotes 4B
T13115 497-498 -LRB- denotes (
T13117 498-506 NNP denotes Amersham
T13118 507-518 NNP denotes Biosciences
T13116 519-524 NNP denotes Corp.
T13119 524-525 -RRB- denotes )
T13120 525-526 . denotes .
T13121 526-605 sentence denotes Each sample was separated on 10% SDS-PAGE, and transferred to a PVDF membrane.
T13122 527-531 DT denotes Each
T13123 532-538 NN denotes sample
T13125 539-542 VBD denotes was
T13124 543-552 VBN denotes separated
T13126 553-555 IN denotes on
T13127 556-558 CD denotes 10
T13128 558-559 NN denotes %
T13130 560-563 NN denotes SDS
T13131 563-564 HYPH denotes -
T13129 564-568 NN denotes PAGE
T13132 568-570 , denotes ,
T13133 570-573 CC denotes and
T13134 574-585 VBN denotes transferred
T13135 586-588 IN denotes to
T13136 589-590 DT denotes a
T13138 591-595 NN denotes PVDF
T13137 596-604 NN denotes membrane
T13139 604-605 . denotes .
T13140 605-714 sentence denotes The blot was then incubated with monoclonal antibody against the ADAM11 (U. S. Patent 5,631,351) at 1 μg/ml.
T13141 606-609 DT denotes The
T13142 610-614 NN denotes blot
T13144 615-618 VBD denotes was
T13145 619-623 RB denotes then
T13143 624-633 VBN denotes incubated
T13146 634-638 IN denotes with
T13147 639-649 JJ denotes monoclonal
T13148 650-658 NN denotes antibody
T13149 659-666 IN denotes against
T13150 667-670 DT denotes the
T13151 671-677 NN denotes ADAM11
T13152 678-679 -LRB- denotes (
T13154 679-681 NNP denotes U.
T13155 682-684 NNP denotes S.
T13153 685-691 NN denotes Patent
T13156 692-701 CD denotes 5,631,351
T13157 701-702 -RRB- denotes )
T13158 703-705 IN denotes at
T13159 706-707 CD denotes 1
T13160 708-710 NN denotes μg
T13161 710-711 SYM denotes /
T13162 711-713 NN denotes ml
T13163 713-714 . denotes .
T13164 714-881 sentence denotes Bound antibodies were visualised with horseradish peroxidase-labelled second antibody and an ECL-plus chemiluminescence detection system (Amersham Biosciences Corp.).
T13165 715-720 JJ denotes Bound
T13166 721-731 NNS denotes antibodies
T13168 732-736 VBD denotes were
T13167 737-747 VBN denotes visualised
T13169 748-752 IN denotes with
T13170 753-764 NN denotes horseradish
T13171 765-775 NN denotes peroxidase
T13173 775-776 HYPH denotes -
T13172 776-784 VBN denotes labelled
T13175 785-791 JJ denotes second
T13174 792-800 NN denotes antibody
T13176 801-804 CC denotes and
T13177 805-807 DT denotes an
T13179 808-811 NN denotes ECL
T13181 811-812 HYPH denotes -
T13180 812-816 NN denotes plus
T13182 817-834 NN denotes chemiluminescence
T13183 835-844 NN denotes detection
T13178 845-851 NN denotes system
T13184 852-853 -LRB- denotes (
T13186 853-861 NNP denotes Amersham
T13187 862-873 NNP denotes Biosciences
T13185 874-879 NNP denotes Corp.
T13188 879-880 -RRB- denotes )
T13189 880-881 . denotes .
R3838 T13011 T13012 compound Western,blot
R3839 T13012 T13013 compound blot,analysis
R3840 T13015 T13016 det The,absence
R3841 T13016 T13017 nsubjpass absence,confirmed
R3842 T13018 T13016 prep of,absence
R3843 T13019 T13020 compound ADAM11,protein
R3844 T13020 T13018 pobj protein,of
R3845 T13021 T13016 prep in,absence
R3846 T13022 T13023 det the,mice
R3847 T13023 T13021 pobj mice,in
R3848 T13024 T13023 punct (,mice
R3849 T13025 T13026 punct -,-
R3850 T13026 T13023 punct -,mice
R3851 T13027 T13026 punct /,-
R3852 T13028 T13023 punct ),mice
R3853 T13029 T13017 auxpass was,confirmed
R3854 T13030 T13017 agent by,confirmed
R3855 T13031 T13032 compound Western,blot
R3856 T13032 T13033 compound blot,analysis
R3857 T13033 T13030 pobj analysis,by
R3858 T13034 T13017 punct .,confirmed
R3859 T13036 T13037 aux To,be
R3860 T13037 T13038 advcl be,isolated
R3861 T13039 T13037 acomp brief,be
R3862 T13040 T13038 punct ", ",isolated
R3863 T13041 T13042 det the,cerebellum
R3864 T13042 T13038 nsubjpass cerebellum,isolated
R3865 T13043 T13038 auxpass was,isolated
R3866 T13044 T13038 prep from,isolated
R3867 T13045 T13046 det a,mouse
R3868 T13046 T13044 pobj mouse,from
R3869 T13047 T13048 nummod six,month
R3870 T13048 T13049 npadvmod month,old
R3871 T13049 T13046 amod old,mouse
R3872 T13050 T13049 punct -,old
R3873 T13051 T13046 prep of,mouse
R3874 T13052 T13053 det each,genotype
R3875 T13053 T13051 pobj genotype,of
R3876 T13054 T13038 cc and,isolated
R3877 T13055 T13038 conj homogenised,isolated
R3878 T13056 T13055 prep in,homogenised
R3879 T13057 T13058 compound cell,lysis
R3880 T13058 T13059 compound lysis,buffer
R3881 T13059 T13056 pobj buffer,in
R3882 T13060 T13061 punct (,cocktail
R3883 T13061 T13055 parataxis cocktail,homogenised
R3884 T13062 T13063 nummod 50,mM
R3885 T13063 T13064 compound mM,HCl
R3886 T13064 T13061 dep HCl,cocktail
R3887 T13065 T13064 compound Tris,HCl
R3888 T13066 T13064 punct -,HCl
R3889 T13067 T13061 punct ", ",cocktail
R3890 T13068 T13061 dep pH,cocktail
R3891 T13069 T13068 nummod 7.5,pH
R3892 T13070 T13061 punct ", ",cocktail
R3893 T13071 T13072 nummod 100,mM
R3894 T13072 T13073 compound mM,NaCl
R3895 T13073 T13061 dep NaCl,cocktail
R3896 T13074 T13061 punct ", ",cocktail
R3897 T13075 T13076 nummod 1,%
R3898 T13076 T13077 compound %,NP
R3899 T13077 T13061 dep NP,cocktail
R3900 T13078 T13077 punct -,NP
R3901 T13079 T13077 nummod 40,NP
R3902 T13080 T13061 punct ", ",cocktail
R3903 T13081 T13082 compound protease,inhibitor
R3904 T13082 T13061 compound inhibitor,cocktail
R3905 T13083 T13084 punct [,Diagnostics
R3906 T13084 T13061 parataxis Diagnostics,cocktail
R3907 T13085 T13084 compound Roche,Diagnostics
R3908 T13086 T13084 punct ],Diagnostics
R3909 T13087 T13061 punct ),cocktail
R3910 T13088 T13038 punct .,isolated
R3911 T13090 T13091 prep After,concentrated
R3912 T13092 T13090 pobj removal,After
R3913 T13093 T13092 prep of,removal
R3914 T13094 T13095 compound cell,debris
R3915 T13095 T13093 pobj debris,of
R3916 T13096 T13092 prep by,removal
R3917 T13097 T13096 pobj centrifugation,by
R3918 T13098 T13091 punct ", ",concentrated
R3919 T13099 T13100 det the,proteins
R3920 T13100 T13091 nsubjpass proteins,concentrated
R3921 T13101 T13100 amod glycosylated,proteins
R3922 T13102 T13100 prep in,proteins
R3923 T13103 T13104 det the,supernatant
R3924 T13104 T13102 pobj supernatant,in
R3925 T13105 T13091 auxpass were,concentrated
R3926 T13106 T13091 prep by,concentrated
R3927 T13107 T13108 det the,chromatography
R3928 T13108 T13106 pobj chromatography,by
R3929 T13109 T13108 compound affinity,chromatography
R3930 T13110 T13091 advcl using,concentrated
R3931 T13111 T13112 compound Con,4B
R3932 T13112 T13110 dobj 4B,using
R3933 T13113 T13112 compound A,4B
R3934 T13114 T13112 compound Sepharose,4B
R3935 T13115 T13116 punct (,Corp.
R3936 T13116 T13110 parataxis Corp.,using
R3937 T13117 T13116 compound Amersham,Corp.
R3938 T13118 T13116 compound Biosciences,Corp.
R3939 T13119 T13116 punct ),Corp.
R3940 T13120 T13091 punct .,concentrated
R3941 T13122 T13123 det Each,sample
R3942 T13123 T13124 nsubjpass sample,separated
R3943 T13125 T13124 auxpass was,separated
R3944 T13126 T13124 prep on,separated
R3945 T13127 T13128 nummod 10,%
R3946 T13128 T13129 compound %,PAGE
R3947 T13129 T13126 pobj PAGE,on
R3948 T13130 T13129 compound SDS,PAGE
R3949 T13131 T13129 punct -,PAGE
R3950 T13132 T13124 punct ", ",separated
R3951 T13133 T13124 cc and,separated
R3952 T13134 T13124 conj transferred,separated
R3953 T13135 T13134 prep to,transferred
R3954 T13136 T13137 det a,membrane
R3955 T13137 T13135 pobj membrane,to
R3956 T13138 T13137 compound PVDF,membrane
R3957 T13139 T13124 punct .,separated
R3958 T13141 T13142 det The,blot
R3959 T13142 T13143 nsubjpass blot,incubated
R3960 T13144 T13143 auxpass was,incubated
R3961 T13145 T13143 advmod then,incubated
R3962 T13146 T13143 prep with,incubated
R3963 T13147 T13148 amod monoclonal,antibody
R3964 T13148 T13146 pobj antibody,with
R3965 T13149 T13148 prep against,antibody
R3966 T13150 T13151 det the,ADAM11
R3967 T13151 T13149 pobj ADAM11,against
R3968 T13152 T13153 punct (,Patent
R3969 T13153 T13151 parataxis Patent,ADAM11
R3970 T13154 T13155 compound U.,S.
R3971 T13155 T13153 compound S.,Patent
R3972 T13156 T13153 nummod "5,631,351",Patent
R3973 T13157 T13153 punct ),Patent
R3974 T13158 T13148 prep at,antibody
R3975 T13159 T13160 nummod 1,μg
R3976 T13160 T13158 pobj μg,at
R3977 T13161 T13162 punct /,ml
R3978 T13162 T13160 prep ml,μg
R3979 T13163 T13143 punct .,incubated
R3980 T13165 T13166 amod Bound,antibodies
R3981 T13166 T13167 nsubjpass antibodies,visualised
R3982 T13168 T13167 auxpass were,visualised
R3983 T13169 T13167 prep with,visualised
R3984 T13170 T13171 compound horseradish,peroxidase
R3985 T13171 T13172 npadvmod peroxidase,labelled
R3986 T13172 T13174 amod labelled,antibody
R3987 T13173 T13172 punct -,labelled
R3988 T13174 T13169 pobj antibody,with
R3989 T13175 T13174 amod second,antibody
R3990 T13176 T13174 cc and,antibody
R3991 T13177 T13178 det an,system
R3992 T13178 T13174 conj system,antibody
R3993 T13179 T13180 compound ECL,plus
R3994 T13180 T13178 compound plus,system
R3995 T13181 T13180 punct -,plus
R3996 T13182 T13183 compound chemiluminescence,detection
R3997 T13183 T13178 compound detection,system
R3998 T13184 T13185 punct (,Corp.
R3999 T13185 T13167 parataxis Corp.,visualised
R4000 T13186 T13185 compound Amersham,Corp.
R4001 T13187 T13185 compound Biosciences,Corp.
R4002 T13188 T13185 punct ),Corp.
R4003 T13189 T13167 punct .,visualised