craft-sa-dev Home  

PMC:1420314 / 18515-19292 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T12309 0-10 NN denotes Generation
T12310 11-13 IN denotes of
T12311 14-20 NN denotes Adam11
T12312 21-30 JJ denotes deficient
T12313 31-35 NNS denotes mice
T12314 35-127 sentence denotes The linearised targeting vector was electroporated into TT2 embryonic stem (ES) cells [40].
T12315 36-39 DT denotes The
T12317 40-50 VBN denotes linearised
T12318 51-60 NN denotes targeting
T12316 61-67 NN denotes vector
T12320 68-71 VBD denotes was
T12319 72-86 VBN denotes electroporated
T12321 87-91 IN denotes into
T12322 92-95 NN denotes TT2
T12324 96-105 JJ denotes embryonic
T12325 106-110 NN denotes stem
T12326 111-112 -LRB- denotes (
T12327 112-114 NN denotes ES
T12328 114-115 -RRB- denotes )
T12323 116-121 NNS denotes cells
T12329 122-123 -LRB- denotes [
T12330 123-125 CD denotes 40
T12331 125-126 -RRB- denotes ]
T12332 126-127 . denotes .
T12333 127-341 sentence denotes Homologous recombinants were selected by PCR using the following primers, AGN1: 5'-TCGTGCTTTACGGTATCGCCGCTCCCGATT-3' in the PGKneo cassette, and SGN033: 5'-GGACCCGGAAGACATTTGCACGGT-3' outside the targeting vector.
T12334 128-138 JJ denotes Homologous
T12335 139-151 NNS denotes recombinants
T12337 152-156 VBD denotes were
T12336 157-165 VBN denotes selected
T12338 166-168 IN denotes by
T12339 169-172 NN denotes PCR
T12340 173-178 VBG denotes using
T12341 179-182 DT denotes the
T12343 183-192 VBG denotes following
T12342 193-200 NNS denotes primers
T12344 200-202 , denotes ,
T12345 202-206 NN denotes AGN1
T12346 206-208 : denotes :
T12347 208-209 CD denotes 5
T12348 209-210 SYM denotes '
T12349 210-211 HYPH denotes -
T12350 211-241 NN denotes TCGTGCTTTACGGTATCGCCGCTCCCGATT
T12351 241-242 HYPH denotes -
T12352 242-243 CD denotes 3
T12353 243-244 SYM denotes '
T12354 245-247 IN denotes in
T12355 248-251 DT denotes the
T12357 252-258 NN denotes PGKneo
T12356 259-267 NN denotes cassette
T12358 267-269 , denotes ,
T12359 269-272 CC denotes and
T12360 273-279 NN denotes SGN033
T12361 279-281 : denotes :
T12362 281-282 CD denotes 5
T12363 282-283 SYM denotes '
T12364 283-284 HYPH denotes -
T12365 284-308 NN denotes GGACCCGGAAGACATTTGCACGGT
T12366 308-309 HYPH denotes -
T12367 309-310 CD denotes 3
T12368 310-311 SYM denotes '
T12369 312-319 IN denotes outside
T12370 320-323 DT denotes the
T12372 324-333 NN denotes targeting
T12371 334-340 NN denotes vector
T12373 340-341 . denotes .
T12374 341-473 sentence denotes Homologous recombined DNA was efficiently amplified by 35 cycles of the following amplification steps (94°C-30 sec and 68°C-5 min).
T12375 342-352 JJ denotes Homologous
T12377 353-363 VBN denotes recombined
T12376 364-367 NN denotes DNA
T12379 368-371 VBD denotes was
T12380 372-383 RB denotes efficiently
T12378 384-393 VBN denotes amplified
T12381 394-396 IN denotes by
T12382 397-399 CD denotes 35
T12383 400-406 NNS denotes cycles
T12384 407-409 IN denotes of
T12385 410-413 DT denotes the
T12387 414-423 VBG denotes following
T12388 424-437 NN denotes amplification
T12386 438-443 NNS denotes steps
T12389 444-445 -LRB- denotes (
T12390 445-447 CD denotes 94
T12391 447-449 NN denotes °C
T12392 449-450 SYM denotes -
T12393 450-452 CD denotes 30
T12394 453-456 NN denotes sec
T12395 457-460 CC denotes and
T12396 461-463 CD denotes 68
T12397 463-465 NN denotes °C
T12398 465-466 SYM denotes -
T12399 466-467 CD denotes 5
T12400 468-471 NN denotes min
T12401 471-472 -RRB- denotes )
T12402 472-473 . denotes .
T12403 473-572 sentence denotes Correctly targeted ES clones were injected into fertilised ICR mouse eggs at the eight-cell stage.
T12404 474-483 RB denotes Correctly
T12405 484-492 VBN denotes targeted
T12407 493-495 NN denotes ES
T12406 496-502 NNS denotes clones
T12409 503-507 VBD denotes were
T12408 508-516 VBN denotes injected
T12410 517-521 IN denotes into
T12411 522-532 VBN denotes fertilised
T12413 533-536 NN denotes ICR
T12414 537-542 NN denotes mouse
T12412 543-547 NNS denotes eggs
T12415 548-550 IN denotes at
T12416 551-554 DT denotes the
T12418 555-560 CD denotes eight
T12420 560-561 HYPH denotes -
T12419 561-565 NN denotes cell
T12417 566-571 NN denotes stage
T12421 571-572 . denotes .
T12422 572-777 sentence denotes The resulting male chimeras were mated with C57BL/6NCrj females (Charles River Japan, Tokyo, Japan), and resulting heterozygous (+/-) male and female mice were interbred to generate homozygous (-/-) mice.
T12423 573-576 DT denotes The
T12425 577-586 VBG denotes resulting
T12426 587-591 JJ denotes male
T12424 592-600 NNS denotes chimeras
T12428 601-605 VBD denotes were
T12427 606-611 VBN denotes mated
T12429 612-616 IN denotes with
T12430 617-622 NN denotes C57BL
T12432 622-623 HYPH denotes /
T12431 623-628 NN denotes 6NCrj
T12433 629-636 NNS denotes females
T12434 637-638 -LRB- denotes (
T12436 638-645 NNP denotes Charles
T12437 646-651 NNP denotes River
T12435 652-657 NNP denotes Japan
T12438 657-659 , denotes ,
T12439 659-664 NNP denotes Tokyo
T12440 664-666 , denotes ,
T12441 666-671 NNP denotes Japan
T12442 671-672 -RRB- denotes )
T12443 672-674 , denotes ,
T12444 674-677 CC denotes and
T12445 678-687 VBG denotes resulting
T12447 688-700 JJ denotes heterozygous
T12448 701-702 -LRB- denotes (
T12449 702-703 SYM denotes +
T12451 703-704 HYPH denotes /
T12450 704-705 SYM denotes -
T12452 705-706 -RRB- denotes )
T12453 707-711 JJ denotes male
T12454 712-715 CC denotes and
T12455 716-722 JJ denotes female
T12446 723-727 NNS denotes mice
T12457 728-732 VBD denotes were
T12456 733-742 VBN denotes interbred
T12458 743-745 TO denotes to
T12459 746-754 VB denotes generate
T12460 755-765 JJ denotes homozygous
T12462 766-767 -LRB- denotes (
T12463 767-768 SYM denotes -
T12465 768-769 HYPH denotes /
T12464 769-770 SYM denotes -
T12466 770-771 -RRB- denotes )
T12461 772-776 NNS denotes mice
T12467 776-777 . denotes .
R3640 T12310 T12309 prep of,Generation
R3641 T12311 T12312 npadvmod Adam11,deficient
R3642 T12312 T12313 amod deficient,mice
R3643 T12313 T12310 pobj mice,of
R3644 T12315 T12316 det The,vector
R3645 T12316 T12319 nsubjpass vector,electroporated
R3646 T12317 T12316 amod linearised,vector
R3647 T12318 T12316 compound targeting,vector
R3648 T12320 T12319 auxpass was,electroporated
R3649 T12321 T12319 prep into,electroporated
R3650 T12322 T12323 nmod TT2,cells
R3651 T12323 T12321 pobj cells,into
R3652 T12324 T12325 amod embryonic,stem
R3653 T12325 T12323 nmod stem,cells
R3654 T12326 T12325 punct (,stem
R3655 T12327 T12325 appos ES,stem
R3656 T12328 T12323 punct ),cells
R3657 T12329 T12330 punct [,40
R3658 T12330 T12319 parataxis 40,electroporated
R3659 T12331 T12330 punct ],40
R3660 T12332 T12319 punct .,electroporated
R3661 T12334 T12335 amod Homologous,recombinants
R3662 T12335 T12336 nsubjpass recombinants,selected
R3663 T12337 T12336 auxpass were,selected
R3664 T12338 T12336 prep by,selected
R3665 T12339 T12338 pobj PCR,by
R3666 T12340 T12336 advcl using,selected
R3667 T12341 T12342 det the,primers
R3668 T12342 T12340 dobj primers,using
R3669 T12343 T12342 amod following,primers
R3670 T12344 T12342 punct ", ",primers
R3671 T12345 T12342 appos AGN1,primers
R3672 T12346 T12345 punct : ,AGN1
R3673 T12347 T12345 appos 5,AGN1
R3674 T12348 T12347 punct ',5
R3675 T12349 T12347 punct -,5
R3676 T12350 T12347 appos TCGTGCTTTACGGTATCGCCGCTCCCGATT,5
R3677 T12351 T12347 punct -,5
R3678 T12352 T12347 nummod 3,5
R3679 T12353 T12347 punct ',5
R3680 T12354 T12345 prep in,AGN1
R3681 T12355 T12356 det the,cassette
R3682 T12356 T12354 pobj cassette,in
R3683 T12357 T12356 compound PGKneo,cassette
R3684 T12358 T12342 punct ", ",primers
R3685 T12359 T12342 cc and,primers
R3686 T12360 T12342 conj SGN033,primers
R3687 T12361 T12360 punct : ,SGN033
R3688 T12362 T12360 appos 5,SGN033
R3689 T12363 T12362 punct ',5
R3690 T12364 T12362 punct -,5
R3691 T12365 T12362 appos GGACCCGGAAGACATTTGCACGGT,5
R3692 T12366 T12362 punct -,5
R3693 T12367 T12362 nummod 3,5
R3694 T12368 T12362 punct ',5
R3695 T12369 T12360 prep outside,SGN033
R3696 T12370 T12371 det the,vector
R3697 T12371 T12369 pobj vector,outside
R3698 T12372 T12371 compound targeting,vector
R3699 T12373 T12336 punct .,selected
R3700 T12375 T12376 amod Homologous,DNA
R3701 T12376 T12378 nsubjpass DNA,amplified
R3702 T12377 T12376 amod recombined,DNA
R3703 T12379 T12378 auxpass was,amplified
R3704 T12380 T12378 advmod efficiently,amplified
R3705 T12381 T12378 agent by,amplified
R3706 T12382 T12383 nummod 35,cycles
R3707 T12383 T12381 pobj cycles,by
R3708 T12384 T12383 prep of,cycles
R3709 T12385 T12386 det the,steps
R3710 T12386 T12384 pobj steps,of
R3711 T12387 T12386 amod following,steps
R3712 T12388 T12386 compound amplification,steps
R3713 T12389 T12386 punct (,steps
R3714 T12390 T12391 nummod 94,°C
R3715 T12391 T12386 appos °C,steps
R3716 T12392 T12391 punct -,°C
R3717 T12393 T12394 nummod 30,sec
R3718 T12394 T12391 npadvmod sec,°C
R3719 T12395 T12391 cc and,°C
R3720 T12396 T12397 nummod 68,°C
R3721 T12397 T12391 conj °C,°C
R3722 T12398 T12397 punct -,°C
R3723 T12399 T12400 nummod 5,min
R3724 T12400 T12397 npadvmod min,°C
R3725 T12401 T12386 punct ),steps
R3726 T12402 T12378 punct .,amplified
R3727 T12404 T12405 advmod Correctly,targeted
R3728 T12405 T12406 amod targeted,clones
R3729 T12406 T12408 nsubjpass clones,injected
R3730 T12407 T12406 compound ES,clones
R3731 T12409 T12408 auxpass were,injected
R3732 T12410 T12408 prep into,injected
R3733 T12411 T12412 amod fertilised,eggs
R3734 T12412 T12410 pobj eggs,into
R3735 T12413 T12412 compound ICR,eggs
R3736 T12414 T12412 compound mouse,eggs
R3737 T12415 T12408 prep at,injected
R3738 T12416 T12417 det the,stage
R3739 T12417 T12415 pobj stage,at
R3740 T12418 T12419 nummod eight,cell
R3741 T12419 T12417 compound cell,stage
R3742 T12420 T12419 punct -,cell
R3743 T12421 T12408 punct .,injected
R3744 T12423 T12424 det The,chimeras
R3745 T12424 T12427 nsubjpass chimeras,mated
R3746 T12425 T12424 amod resulting,chimeras
R3747 T12426 T12424 amod male,chimeras
R3748 T12428 T12427 auxpass were,mated
R3749 T12429 T12427 prep with,mated
R3750 T12430 T12431 compound C57BL,6NCrj
R3751 T12431 T12433 compound 6NCrj,females
R3752 T12432 T12431 punct /,6NCrj
R3753 T12433 T12429 pobj females,with
R3754 T12434 T12435 punct (,Japan
R3755 T12435 T12427 parataxis Japan,mated
R3756 T12436 T12435 compound Charles,Japan
R3757 T12437 T12435 compound River,Japan
R3758 T12438 T12435 punct ", ",Japan
R3759 T12439 T12435 npadvmod Tokyo,Japan
R3760 T12440 T12435 punct ", ",Japan
R3761 T12441 T12435 npadvmod Japan,Japan
R3762 T12442 T12435 punct ),Japan
R3763 T12443 T12427 punct ", ",mated
R3764 T12444 T12427 cc and,mated
R3765 T12445 T12446 amod resulting,mice
R3766 T12446 T12456 nsubjpass mice,interbred
R3767 T12447 T12446 amod heterozygous,mice
R3768 T12448 T12446 punct (,mice
R3769 T12449 T12450 punct +,-
R3770 T12450 T12446 punct -,mice
R3771 T12451 T12450 punct /,-
R3772 T12452 T12446 punct ),mice
R3773 T12453 T12446 amod male,mice
R3774 T12454 T12453 cc and,male
R3775 T12455 T12453 conj female,male
R3776 T12456 T12427 conj interbred,mated
R3777 T12457 T12456 auxpass were,interbred
R3778 T12458 T12459 aux to,generate
R3779 T12459 T12456 advcl generate,interbred
R3780 T12460 T12461 amod homozygous,mice
R3781 T12461 T12459 dobj mice,generate
R3782 T12462 T12461 punct (,mice
R3783 T12463 T12464 punct -,-
R3784 T12464 T12461 punct -,mice
R3785 T12465 T12464 punct /,-
R3786 T12466 T12461 punct ),mice
R3787 T12467 T12456 punct .,interbred