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PMC:1420314 / 17967-18513 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T11873 0-3 DT denotes The
T11875 4-8 CD denotes 13.7
T11877 8-9 HYPH denotes -
T11876 9-11 NN denotes kb
T11878 12-17 NN denotes BamHI
T11879 18-21 NN denotes DNA
T11874 22-30 NN denotes fragment
T11881 31-39 VBG denotes covering
T11882 40-45 NNS denotes exons
T11883 46-47 CD denotes 2
T11884 48-49 SYM denotes –
T11885 50-52 CD denotes 20
T11886 53-55 IN denotes of
T11887 56-59 DT denotes the
T11889 60-66 NN denotes Adam11
T11888 67-71 NN denotes gene
T11890 72-75 VBD denotes was
T11880 76-85 VBN denotes amplified
T11891 86-90 IN denotes from
T11892 91-96 NN denotes C57BL
T11894 96-97 HYPH denotes /
T11895 97-98 CD denotes 6
T11896 99-106 JJ denotes genomic
T11893 107-110 NN denotes DNA
T11897 111-113 IN denotes by
T11898 114-117 DT denotes the
T11899 118-121 NN denotes PCR
T11900 122-127 VBG denotes using
T11901 128-134 NN denotes Expand
T11903 135-137 NNP denotes Hi
T11905 137-138 HYPH denotes -
T11904 138-146 NNP denotes Fidelity
T11906 147-157 NN denotes polymerase
T11902 158-161 NN denotes mix
T11907 162-163 -LRB- denotes (
T11909 163-168 NNP denotes Roche
T11910 169-180 NNP denotes Diagnostics
T11908 181-183 NNP denotes KK
T11911 183-185 , denotes ,
T11912 185-190 NNP denotes Tokyo
T11913 190-192 , denotes ,
T11914 192-197 NNP denotes Japan
T11915 197-198 -RRB- denotes )
T11916 198-199 . denotes .
T11917 199-315 sentence denotes To generate a mutation in the mouse Adam11 gene, we inserted a PGKneo cassette into exons 5 – 7 of the Adam11 gene.
T11918 200-202 TO denotes To
T11919 203-211 VB denotes generate
T11921 212-213 DT denotes a
T11922 214-222 NN denotes mutation
T11923 223-225 IN denotes in
T11924 226-229 DT denotes the
T11926 230-235 NN denotes mouse
T11927 236-242 NN denotes Adam11
T11925 243-247 NN denotes gene
T11928 247-249 , denotes ,
T11929 249-251 PRP denotes we
T11920 252-260 VBD denotes inserted
T11930 261-262 DT denotes a
T11932 263-269 NN denotes PGKneo
T11931 270-278 NN denotes cassette
T11933 279-283 IN denotes into
T11934 284-289 NNS denotes exons
T11935 290-291 CD denotes 5
T11936 292-293 SYM denotes –
T11937 294-295 CD denotes 7
T11938 296-298 IN denotes of
T11939 299-302 DT denotes the
T11941 303-309 NN denotes Adam11
T11940 310-314 NN denotes gene
T11942 314-315 . denotes .
T11943 315-546 sentence denotes The final targeting construct consisted of a 10.1-kb genomic DNA fragment that was interrupted by the insertion of the PGKneo cassette and contained a MC1/TK cassette as a negative selection marker against random integration [39].
T11944 316-319 DT denotes The
T11946 320-325 JJ denotes final
T11947 326-335 NN denotes targeting
T11945 336-345 NN denotes construct
T11948 346-355 VBD denotes consisted
T11949 356-358 IN denotes of
T11950 359-360 DT denotes a
T11952 361-365 CD denotes 10.1
T11954 365-366 HYPH denotes -
T11953 366-368 NN denotes kb
T11955 369-376 JJ denotes genomic
T11956 377-380 NN denotes DNA
T11951 381-389 NN denotes fragment
T11957 390-394 WDT denotes that
T11959 395-398 VBD denotes was
T11958 399-410 VBN denotes interrupted
T11960 411-413 IN denotes by
T11961 414-417 DT denotes the
T11962 418-427 NN denotes insertion
T11963 428-430 IN denotes of
T11964 431-434 DT denotes the
T11966 435-441 NN denotes PGKneo
T11965 442-450 NN denotes cassette
T11967 451-454 CC denotes and
T11968 455-464 VBD denotes contained
T11969 465-466 DT denotes a
T11971 467-470 NN denotes MC1
T11973 470-471 HYPH denotes /
T11972 471-473 NN denotes TK
T11970 474-482 NN denotes cassette
T11974 483-485 IN denotes as
T11975 486-487 DT denotes a
T11977 488-496 JJ denotes negative
T11978 497-506 NN denotes selection
T11976 507-513 NN denotes marker
T11979 514-521 IN denotes against
T11980 522-528 JJ denotes random
T11981 529-540 NN denotes integration
T11982 541-542 -LRB- denotes [
T11983 542-544 CD denotes 39
T11984 544-545 -RRB- denotes ]
T11985 545-546 . denotes .
R3528 T11873 T11874 det The,fragment
R3529 T11874 T11880 nsubjpass fragment,amplified
R3530 T11875 T11876 nummod 13.7,kb
R3531 T11876 T11874 compound kb,fragment
R3532 T11877 T11876 punct -,kb
R3533 T11878 T11874 compound BamHI,fragment
R3534 T11879 T11874 compound DNA,fragment
R3535 T11881 T11874 acl covering,fragment
R3536 T11882 T11883 nmod exons,2
R3537 T11883 T11881 dobj 2,covering
R3538 T11884 T11885 punct –,20
R3539 T11885 T11883 prep 20,2
R3540 T11886 T11883 prep of,2
R3541 T11887 T11888 det the,gene
R3542 T11888 T11886 pobj gene,of
R3543 T11889 T11888 compound Adam11,gene
R3544 T11890 T11880 auxpass was,amplified
R3545 T11891 T11880 prep from,amplified
R3546 T11892 T11893 nmod C57BL,DNA
R3547 T11893 T11891 pobj DNA,from
R3548 T11894 T11892 punct /,C57BL
R3549 T11895 T11892 nummod 6,C57BL
R3550 T11896 T11893 amod genomic,DNA
R3551 T11897 T11880 prep by,amplified
R3552 T11898 T11899 det the,PCR
R3553 T11899 T11897 pobj PCR,by
R3554 T11900 T11899 acl using,PCR
R3555 T11901 T11902 compound Expand,mix
R3556 T11902 T11900 dobj mix,using
R3557 T11903 T11904 compound Hi,Fidelity
R3558 T11904 T11902 compound Fidelity,mix
R3559 T11905 T11904 punct -,Fidelity
R3560 T11906 T11902 compound polymerase,mix
R3561 T11907 T11908 punct (,KK
R3562 T11908 T11902 parataxis KK,mix
R3563 T11909 T11908 compound Roche,KK
R3564 T11910 T11908 compound Diagnostics,KK
R3565 T11911 T11908 punct ", ",KK
R3566 T11912 T11908 npadvmod Tokyo,KK
R3567 T11913 T11908 punct ", ",KK
R3568 T11914 T11908 npadvmod Japan,KK
R3569 T11915 T11908 punct ),KK
R3570 T11916 T11880 punct .,amplified
R3571 T11918 T11919 aux To,generate
R3572 T11919 T11920 advcl generate,inserted
R3573 T11921 T11922 det a,mutation
R3574 T11922 T11919 dobj mutation,generate
R3575 T11923 T11919 prep in,generate
R3576 T11924 T11925 det the,gene
R3577 T11925 T11923 pobj gene,in
R3578 T11926 T11925 compound mouse,gene
R3579 T11927 T11925 compound Adam11,gene
R3580 T11928 T11920 punct ", ",inserted
R3581 T11929 T11920 nsubj we,inserted
R3582 T11930 T11931 det a,cassette
R3583 T11931 T11920 dobj cassette,inserted
R3584 T11932 T11931 compound PGKneo,cassette
R3585 T11933 T11920 prep into,inserted
R3586 T11934 T11935 nmod exons,5
R3587 T11935 T11933 pobj 5,into
R3588 T11936 T11937 punct –,7
R3589 T11937 T11935 prep 7,5
R3590 T11938 T11935 prep of,5
R3591 T11939 T11940 det the,gene
R3592 T11940 T11938 pobj gene,of
R3593 T11941 T11940 compound Adam11,gene
R3594 T11942 T11920 punct .,inserted
R3595 T11944 T11945 det The,construct
R3596 T11945 T11948 nsubj construct,consisted
R3597 T11946 T11945 amod final,construct
R3598 T11947 T11945 compound targeting,construct
R3599 T11949 T11948 prep of,consisted
R3600 T11950 T11951 det a,fragment
R3601 T11951 T11949 pobj fragment,of
R3602 T11952 T11953 nummod 10.1,kb
R3603 T11953 T11951 nmod kb,fragment
R3604 T11954 T11953 punct -,kb
R3605 T11955 T11951 amod genomic,fragment
R3606 T11956 T11951 compound DNA,fragment
R3607 T11957 T11958 dep that,interrupted
R3608 T11958 T11951 relcl interrupted,fragment
R3609 T11959 T11958 auxpass was,interrupted
R3610 T11960 T11958 agent by,interrupted
R3611 T11961 T11962 det the,insertion
R3612 T11962 T11960 pobj insertion,by
R3613 T11963 T11962 prep of,insertion
R3614 T11964 T11965 det the,cassette
R3615 T11965 T11963 pobj cassette,of
R3616 T11966 T11965 compound PGKneo,cassette
R3617 T11967 T11958 cc and,interrupted
R3618 T11968 T11958 conj contained,interrupted
R3619 T11969 T11970 det a,cassette
R3620 T11970 T11968 dobj cassette,contained
R3621 T11971 T11972 compound MC1,TK
R3622 T11972 T11970 compound TK,cassette
R3623 T11973 T11972 punct /,TK
R3624 T11974 T11968 prep as,contained
R3625 T11975 T11976 det a,marker
R3626 T11976 T11974 pobj marker,as
R3627 T11977 T11978 amod negative,selection
R3628 T11978 T11976 compound selection,marker
R3629 T11979 T11976 prep against,marker
R3630 T11980 T11981 amod random,integration
R3631 T11981 T11979 pobj integration,against
R3632 T11982 T11983 punct [,39
R3633 T11983 T11968 parataxis 39,contained
R3634 T11984 T11983 punct ],39
R3635 T11985 T11948 punct .,consisted