| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T4280 |
0-2 |
TO |
denotes |
To |
| T4281 |
3-9 |
VB |
denotes |
obtain |
| T4283 |
10-13 |
NN |
denotes |
DNA |
| T4284 |
14-23 |
NNS |
denotes |
fragments |
| T4285 |
24-34 |
VBG |
denotes |
containing |
| T4286 |
35-38 |
DT |
denotes |
the |
| T4288 |
39-43 |
NN |
denotes |
ESG1 |
| T4287 |
44-48 |
NN |
denotes |
gene |
| T4289 |
48-50 |
, |
denotes |
, |
| T4290 |
50-52 |
PRP |
denotes |
we |
| T4282 |
53-61 |
VBD |
denotes |
screened |
| T4291 |
62-65 |
DT |
denotes |
the |
| T4293 |
66-75 |
JJ |
denotes |
bacterial |
| T4295 |
76-86 |
JJ |
denotes |
artificial |
| T4294 |
87-97 |
NN |
denotes |
chromosome |
| T4296 |
98-99 |
-LRB- |
denotes |
( |
| T4297 |
99-102 |
NN |
denotes |
BAC |
| T4298 |
102-103 |
-RRB- |
denotes |
) |
| T4299 |
104-107 |
NN |
denotes |
DNA |
| T4292 |
108-112 |
NN |
denotes |
pool |
| T4300 |
113-115 |
IN |
denotes |
by |
| T4301 |
116-119 |
NN |
denotes |
PCR |
| T4302 |
120-125 |
VBG |
denotes |
using |
| T4303 |
126-132 |
NN |
denotes |
primer |
| T4304 |
133-138 |
NNS |
denotes |
pairs |
| T4305 |
139-143 |
WDT |
denotes |
that |
| T4307 |
144-149 |
MD |
denotes |
would |
| T4308 |
150-154 |
RB |
denotes |
only |
| T4306 |
155-162 |
VB |
denotes |
amplify |
| T4309 |
163-166 |
DT |
denotes |
the |
| T4311 |
167-171 |
JJ |
denotes |
real |
| T4310 |
172-176 |
NN |
denotes |
gene |
| T4312 |
176-178 |
, |
denotes |
, |
| T4313 |
178-181 |
RB |
denotes |
not |
| T4315 |
182-185 |
DT |
denotes |
the |
| T4314 |
186-197 |
NNS |
denotes |
pseudogenes |
| T4316 |
197-198 |
. |
denotes |
. |
| T4317 |
198-255 |
sentence |
denotes |
We obtained two independent, but overlapping BAC clones. |
| T4318 |
199-201 |
PRP |
denotes |
We |
| T4319 |
202-210 |
VBD |
denotes |
obtained |
| T4320 |
211-214 |
CD |
denotes |
two |
| T4322 |
215-226 |
JJ |
denotes |
independent |
| T4323 |
226-228 |
, |
denotes |
, |
| T4324 |
228-231 |
CC |
denotes |
but |
| T4325 |
232-243 |
VBG |
denotes |
overlapping |
| T4326 |
244-247 |
NN |
denotes |
BAC |
| T4321 |
248-254 |
NNS |
denotes |
clones |
| T4327 |
254-255 |
. |
denotes |
. |
| T4328 |
255-447 |
sentence |
denotes |
Southern blot analyses and sequencing demonstrated that these clones contained a sequence exhibiting complete identity with ESG1 cDNA that was interrupted by two putative introns (Figure 2A). |
| T4329 |
256-264 |
NNP |
denotes |
Southern |
| T4330 |
265-269 |
NN |
denotes |
blot |
| T4332 |
270-278 |
NNS |
denotes |
analyses |
| T4333 |
279-282 |
CC |
denotes |
and |
| T4334 |
283-293 |
NN |
denotes |
sequencing |
| T4331 |
294-306 |
VBD |
denotes |
demonstrated |
| T4335 |
307-311 |
IN |
denotes |
that |
| T4337 |
312-317 |
DT |
denotes |
these |
| T4338 |
318-324 |
NNS |
denotes |
clones |
| T4336 |
325-334 |
VBD |
denotes |
contained |
| T4339 |
335-336 |
DT |
denotes |
a |
| T4340 |
337-345 |
NN |
denotes |
sequence |
| T4341 |
346-356 |
VBG |
denotes |
exhibiting |
| T4342 |
357-365 |
JJ |
denotes |
complete |
| T4343 |
366-374 |
NN |
denotes |
identity |
| T4344 |
375-379 |
IN |
denotes |
with |
| T4345 |
380-384 |
NN |
denotes |
ESG1 |
| T4346 |
385-389 |
NN |
denotes |
cDNA |
| T4347 |
390-394 |
WDT |
denotes |
that |
| T4349 |
395-398 |
VBD |
denotes |
was |
| T4348 |
399-410 |
VBN |
denotes |
interrupted |
| T4350 |
411-413 |
IN |
denotes |
by |
| T4351 |
414-417 |
CD |
denotes |
two |
| T4353 |
418-426 |
JJ |
denotes |
putative |
| T4352 |
427-434 |
NNS |
denotes |
introns |
| T4354 |
435-436 |
-LRB- |
denotes |
( |
| T4356 |
436-442 |
NN |
denotes |
Figure |
| T4355 |
443-445 |
NN |
denotes |
2A |
| T4357 |
445-446 |
-RRB- |
denotes |
) |
| T4358 |
446-447 |
. |
denotes |
. |
| T4359 |
447-566 |
sentence |
denotes |
The two intron sequences begin with GT and terminate with AG, fulfilling the GT-AG rule of exon-intron junctions [19]. |
| T4360 |
448-451 |
DT |
denotes |
The |
| T4362 |
452-455 |
CD |
denotes |
two |
| T4363 |
456-462 |
NN |
denotes |
intron |
| T4361 |
463-472 |
NNS |
denotes |
sequences |
| T4364 |
473-478 |
VBP |
denotes |
begin |
| T4365 |
479-483 |
IN |
denotes |
with |
| T4366 |
484-486 |
NN |
denotes |
GT |
| T4367 |
487-490 |
CC |
denotes |
and |
| T4368 |
491-500 |
VB |
denotes |
terminate |
| T4369 |
501-505 |
IN |
denotes |
with |
| T4370 |
506-508 |
NN |
denotes |
AG |
| T4371 |
508-510 |
, |
denotes |
, |
| T4372 |
510-520 |
VBG |
denotes |
fulfilling |
| T4373 |
521-524 |
DT |
denotes |
the |
| T4375 |
525-527 |
NN |
denotes |
GT |
| T4377 |
527-528 |
HYPH |
denotes |
- |
| T4376 |
528-530 |
NN |
denotes |
AG |
| T4374 |
531-535 |
NN |
denotes |
rule |
| T4378 |
536-538 |
IN |
denotes |
of |
| T4379 |
539-543 |
NN |
denotes |
exon |
| T4381 |
543-544 |
HYPH |
denotes |
- |
| T4380 |
544-550 |
NN |
denotes |
intron |
| T4382 |
551-560 |
NNS |
denotes |
junctions |
| T4383 |
561-562 |
-LRB- |
denotes |
[ |
| T4384 |
562-564 |
CD |
denotes |
19 |
| T4385 |
564-565 |
-RRB- |
denotes |
] |
| T4386 |
565-566 |
. |
denotes |
. |
| T4387 |
566-751 |
sentence |
denotes |
The 5' flanking region of this DNA fragment exhibited strong promoter/enhancer activity by luciferase reporter assays in undifferentiated ES cells, but not in somatic cells (Figure 3). |
| T4388 |
567-570 |
DT |
denotes |
The |
| T4390 |
571-572 |
CD |
denotes |
5 |
| T4391 |
572-573 |
SYM |
denotes |
' |
| T4392 |
574-582 |
NN |
denotes |
flanking |
| T4389 |
583-589 |
NN |
denotes |
region |
| T4394 |
590-592 |
IN |
denotes |
of |
| T4395 |
593-597 |
DT |
denotes |
this |
| T4397 |
598-601 |
NN |
denotes |
DNA |
| T4396 |
602-610 |
NN |
denotes |
fragment |
| T4393 |
611-620 |
VBD |
denotes |
exhibited |
| T4398 |
621-627 |
JJ |
denotes |
strong |
| T4400 |
628-636 |
NN |
denotes |
promoter |
| T4402 |
636-637 |
HYPH |
denotes |
/ |
| T4401 |
637-645 |
NN |
denotes |
enhancer |
| T4399 |
646-654 |
NN |
denotes |
activity |
| T4403 |
655-657 |
IN |
denotes |
by |
| T4404 |
658-668 |
NN |
denotes |
luciferase |
| T4406 |
669-677 |
NN |
denotes |
reporter |
| T4405 |
678-684 |
NNS |
denotes |
assays |
| T4407 |
685-687 |
IN |
denotes |
in |
| T4408 |
688-704 |
JJ |
denotes |
undifferentiated |
| T4410 |
705-707 |
NN |
denotes |
ES |
| T4409 |
708-713 |
NNS |
denotes |
cells |
| T4411 |
713-715 |
, |
denotes |
, |
| T4412 |
715-718 |
CC |
denotes |
but |
| T4413 |
719-722 |
RB |
denotes |
not |
| T4414 |
723-725 |
IN |
denotes |
in |
| T4415 |
726-733 |
JJ |
denotes |
somatic |
| T4416 |
734-739 |
NNS |
denotes |
cells |
| T4417 |
740-741 |
-LRB- |
denotes |
( |
| T4418 |
741-747 |
NN |
denotes |
Figure |
| T4419 |
748-749 |
CD |
denotes |
3 |
| T4420 |
749-750 |
-RRB- |
denotes |
) |
| T4421 |
750-751 |
. |
denotes |
. |
| T4422 |
751-850 |
sentence |
denotes |
The same fragment showed much weaker activity after induction of differentiation by retinoic acid. |
| T4423 |
752-755 |
DT |
denotes |
The |
| T4425 |
756-760 |
JJ |
denotes |
same |
| T4424 |
761-769 |
NN |
denotes |
fragment |
| T4426 |
770-776 |
VBD |
denotes |
showed |
| T4427 |
777-781 |
RB |
denotes |
much |
| T4428 |
782-788 |
JJR |
denotes |
weaker |
| T4429 |
789-797 |
NN |
denotes |
activity |
| T4430 |
798-803 |
IN |
denotes |
after |
| T4431 |
804-813 |
NN |
denotes |
induction |
| T4432 |
814-816 |
IN |
denotes |
of |
| T4433 |
817-832 |
NN |
denotes |
differentiation |
| T4434 |
833-835 |
IN |
denotes |
by |
| T4435 |
836-844 |
JJ |
denotes |
retinoic |
| T4436 |
845-849 |
NN |
denotes |
acid |
| T4437 |
849-850 |
. |
denotes |
. |
| T4438 |
850-900 |
sentence |
denotes |
We concluded that this sequence is the ESG1 gene. |
| T4439 |
851-853 |
PRP |
denotes |
We |
| T4440 |
854-863 |
VBD |
denotes |
concluded |
| T4441 |
864-868 |
IN |
denotes |
that |
| T4443 |
869-873 |
DT |
denotes |
this |
| T4444 |
874-882 |
NN |
denotes |
sequence |
| T4442 |
883-885 |
VBZ |
denotes |
is |
| T4445 |
886-889 |
DT |
denotes |
the |
| T4447 |
890-894 |
NN |
denotes |
ESG1 |
| T4446 |
895-899 |
NN |
denotes |
gene |
| T4448 |
899-900 |
. |
denotes |
. |
| R1000 |
T4312 |
T4306 |
punct |
", ",amplify |
| R1001 |
T4313 |
T4314 |
neg |
not,pseudogenes |
| R1002 |
T4314 |
T4306 |
dep |
pseudogenes,amplify |
| R1003 |
T4315 |
T4314 |
det |
the,pseudogenes |
| R1004 |
T4316 |
T4282 |
punct |
.,screened |
| R1005 |
T4318 |
T4319 |
nsubj |
We,obtained |
| R1006 |
T4320 |
T4321 |
nummod |
two,clones |
| R1007 |
T4321 |
T4319 |
dobj |
clones,obtained |
| R1008 |
T4322 |
T4321 |
amod |
independent,clones |
| R1009 |
T4323 |
T4322 |
punct |
", ",independent |
| R1010 |
T4324 |
T4322 |
cc |
but,independent |
| R1011 |
T4325 |
T4322 |
conj |
overlapping,independent |
| R1012 |
T4326 |
T4321 |
compound |
BAC,clones |
| R1013 |
T4327 |
T4319 |
punct |
.,obtained |
| R1014 |
T4329 |
T4330 |
compound |
Southern,blot |
| R1015 |
T4330 |
T4331 |
nsubj |
blot,demonstrated |
| R1016 |
T4332 |
T4330 |
appos |
analyses,blot |
| R1017 |
T4333 |
T4332 |
cc |
and,analyses |
| R1018 |
T4334 |
T4332 |
conj |
sequencing,analyses |
| R1019 |
T4335 |
T4336 |
mark |
that,contained |
| R1020 |
T4336 |
T4331 |
ccomp |
contained,demonstrated |
| R1021 |
T4337 |
T4338 |
det |
these,clones |
| R1022 |
T4338 |
T4336 |
nsubj |
clones,contained |
| R1023 |
T4339 |
T4340 |
det |
a,sequence |
| R1024 |
T4340 |
T4336 |
dobj |
sequence,contained |
| R1025 |
T4341 |
T4340 |
acl |
exhibiting,sequence |
| R1026 |
T4342 |
T4343 |
amod |
complete,identity |
| R1027 |
T4343 |
T4341 |
dobj |
identity,exhibiting |
| R1028 |
T4344 |
T4343 |
prep |
with,identity |
| R1029 |
T4345 |
T4346 |
compound |
ESG1,cDNA |
| R1030 |
T4346 |
T4344 |
pobj |
cDNA,with |
| R1031 |
T4347 |
T4348 |
dep |
that,interrupted |
| R1032 |
T4348 |
T4340 |
relcl |
interrupted,sequence |
| R1033 |
T4349 |
T4348 |
auxpass |
was,interrupted |
| R1034 |
T4350 |
T4348 |
agent |
by,interrupted |
| R1035 |
T4351 |
T4352 |
nummod |
two,introns |
| R1036 |
T4352 |
T4350 |
pobj |
introns,by |
| R1037 |
T4353 |
T4352 |
amod |
putative,introns |
| R1038 |
T4354 |
T4355 |
punct |
(,2A |
| R1039 |
T4355 |
T4348 |
parataxis |
2A,interrupted |
| R1040 |
T4356 |
T4355 |
compound |
Figure,2A |
| R1041 |
T4357 |
T4355 |
punct |
),2A |
| R1042 |
T4358 |
T4331 |
punct |
.,demonstrated |
| R1043 |
T4360 |
T4361 |
det |
The,sequences |
| R1044 |
T4361 |
T4364 |
nsubj |
sequences,begin |
| R1045 |
T4362 |
T4361 |
nummod |
two,sequences |
| R1046 |
T4363 |
T4361 |
compound |
intron,sequences |
| R1047 |
T4365 |
T4364 |
prep |
with,begin |
| R1048 |
T4366 |
T4365 |
pobj |
GT,with |
| R1049 |
T4367 |
T4364 |
cc |
and,begin |
| R1050 |
T4368 |
T4364 |
conj |
terminate,begin |
| R1051 |
T4369 |
T4368 |
prep |
with,terminate |
| R1052 |
T4370 |
T4369 |
pobj |
AG,with |
| R1053 |
T4371 |
T4364 |
punct |
", ",begin |
| R1054 |
T4372 |
T4364 |
advcl |
fulfilling,begin |
| R1055 |
T4373 |
T4374 |
det |
the,rule |
| R1056 |
T4374 |
T4372 |
dobj |
rule,fulfilling |
| R1057 |
T4375 |
T4376 |
compound |
GT,AG |
| R1058 |
T4376 |
T4374 |
compound |
AG,rule |
| R1059 |
T4377 |
T4376 |
punct |
-,AG |
| R1060 |
T4378 |
T4374 |
prep |
of,rule |
| R1061 |
T4379 |
T4380 |
compound |
exon,intron |
| R1062 |
T4380 |
T4382 |
compound |
intron,junctions |
| R1063 |
T4381 |
T4380 |
punct |
-,intron |
| R1064 |
T4382 |
T4378 |
pobj |
junctions,of |
| R1065 |
T4383 |
T4384 |
punct |
[,19 |
| R1066 |
T4384 |
T4372 |
parataxis |
19,fulfilling |
| R1067 |
T4385 |
T4384 |
punct |
],19 |
| R1068 |
T4386 |
T4364 |
punct |
.,begin |
| R1069 |
T4388 |
T4389 |
det |
The,region |
| R1070 |
T4389 |
T4393 |
nsubj |
region,exhibited |
| R1071 |
T4390 |
T4389 |
nummod |
5,region |
| R1072 |
T4391 |
T4390 |
punct |
',5 |
| R1073 |
T4392 |
T4389 |
compound |
flanking,region |
| R1074 |
T4394 |
T4389 |
prep |
of,region |
| R1075 |
T4395 |
T4396 |
det |
this,fragment |
| R1076 |
T4396 |
T4394 |
pobj |
fragment,of |
| R1077 |
T4397 |
T4396 |
compound |
DNA,fragment |
| R1078 |
T4398 |
T4399 |
amod |
strong,activity |
| R1079 |
T4399 |
T4393 |
dobj |
activity,exhibited |
| R1080 |
T4400 |
T4401 |
compound |
promoter,enhancer |
| R1081 |
T4401 |
T4399 |
compound |
enhancer,activity |
| R1082 |
T4402 |
T4401 |
punct |
/,enhancer |
| R1083 |
T4403 |
T4393 |
prep |
by,exhibited |
| R1084 |
T4404 |
T4405 |
compound |
luciferase,assays |
| R1085 |
T4405 |
T4403 |
pobj |
assays,by |
| R1086 |
T4406 |
T4405 |
compound |
reporter,assays |
| R1087 |
T4407 |
T4393 |
prep |
in,exhibited |
| R1088 |
T4408 |
T4409 |
amod |
undifferentiated,cells |
| R1089 |
T4409 |
T4407 |
pobj |
cells,in |
| R1090 |
T4410 |
T4409 |
compound |
ES,cells |
| R1091 |
T4411 |
T4407 |
punct |
", ",in |
| R1092 |
T4412 |
T4407 |
cc |
but,in |
| R1093 |
T4413 |
T4412 |
neg |
not,but |
| R1094 |
T4414 |
T4407 |
conj |
in,in |
| R1095 |
T4415 |
T4416 |
amod |
somatic,cells |
| R1096 |
T4416 |
T4414 |
pobj |
cells,in |
| R1097 |
T4417 |
T4418 |
punct |
(,Figure |
| R1098 |
T4418 |
T4393 |
parataxis |
Figure,exhibited |
| R1099 |
T4419 |
T4418 |
nummod |
3,Figure |
| R1100 |
T4420 |
T4418 |
punct |
),Figure |
| R1101 |
T4421 |
T4393 |
punct |
.,exhibited |
| R1102 |
T4423 |
T4424 |
det |
The,fragment |
| R1103 |
T4424 |
T4426 |
nsubj |
fragment,showed |
| R1104 |
T4425 |
T4424 |
amod |
same,fragment |
| R1105 |
T4427 |
T4428 |
advmod |
much,weaker |
| R1106 |
T4428 |
T4429 |
amod |
weaker,activity |
| R1107 |
T4429 |
T4426 |
dobj |
activity,showed |
| R1108 |
T4430 |
T4426 |
prep |
after,showed |
| R1109 |
T4431 |
T4430 |
pobj |
induction,after |
| R1110 |
T4432 |
T4431 |
prep |
of,induction |
| R1111 |
T4433 |
T4432 |
pobj |
differentiation,of |
| R1112 |
T4434 |
T4431 |
prep |
by,induction |
| R1113 |
T4435 |
T4436 |
amod |
retinoic,acid |
| R1114 |
T4436 |
T4434 |
pobj |
acid,by |
| R1115 |
T4437 |
T4426 |
punct |
.,showed |
| R1116 |
T4439 |
T4440 |
nsubj |
We,concluded |
| R1117 |
T4441 |
T4442 |
mark |
that,is |
| R1118 |
T4442 |
T4440 |
ccomp |
is,concluded |
| R1119 |
T4443 |
T4444 |
det |
this,sequence |
| R1120 |
T4444 |
T4442 |
nsubj |
sequence,is |
| R1121 |
T4445 |
T4446 |
det |
the,gene |
| R1122 |
T4446 |
T4442 |
attr |
gene,is |
| R1123 |
T4447 |
T4446 |
compound |
ESG1,gene |
| R1124 |
T4448 |
T4440 |
punct |
.,concluded |
| R969 |
T4280 |
T4281 |
aux |
To,obtain |
| R970 |
T4281 |
T4282 |
advcl |
obtain,screened |
| R971 |
T4283 |
T4284 |
compound |
DNA,fragments |
| R972 |
T4284 |
T4281 |
dobj |
fragments,obtain |
| R973 |
T4285 |
T4284 |
acl |
containing,fragments |
| R974 |
T4286 |
T4287 |
det |
the,gene |
| R975 |
T4287 |
T4285 |
dobj |
gene,containing |
| R976 |
T4288 |
T4287 |
compound |
ESG1,gene |
| R977 |
T4289 |
T4282 |
punct |
", ",screened |
| R978 |
T4290 |
T4282 |
nsubj |
we,screened |
| R979 |
T4291 |
T4292 |
det |
the,pool |
| R980 |
T4292 |
T4282 |
dobj |
pool,screened |
| R981 |
T4293 |
T4294 |
amod |
bacterial,chromosome |
| R982 |
T4294 |
T4292 |
nmod |
chromosome,pool |
| R983 |
T4295 |
T4294 |
amod |
artificial,chromosome |
| R984 |
T4296 |
T4294 |
punct |
(,chromosome |
| R985 |
T4297 |
T4294 |
appos |
BAC,chromosome |
| R986 |
T4298 |
T4292 |
punct |
),pool |
| R987 |
T4299 |
T4292 |
compound |
DNA,pool |
| R988 |
T4300 |
T4282 |
prep |
by,screened |
| R989 |
T4301 |
T4300 |
pobj |
PCR,by |
| R990 |
T4302 |
T4282 |
advcl |
using,screened |
| R991 |
T4303 |
T4304 |
compound |
primer,pairs |
| R992 |
T4304 |
T4302 |
dobj |
pairs,using |
| R993 |
T4305 |
T4306 |
dep |
that,amplify |
| R994 |
T4306 |
T4304 |
relcl |
amplify,pairs |
| R995 |
T4307 |
T4306 |
aux |
would,amplify |
| R996 |
T4308 |
T4306 |
advmod |
only,amplify |
| R997 |
T4309 |
T4310 |
det |
the,gene |
| R998 |
T4310 |
T4306 |
dobj |
gene,amplify |
| R999 |
T4311 |
T4310 |
amod |
real,gene |
