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PMC:1420271 / 19922-21056 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T11476 0-10 NN denotes Derivation
T11477 11-13 IN denotes of
T11478 14-18 NN denotes ESG1
T11480 18-19 HYPH denotes -
T11479 19-28 JJ denotes deficient
T11482 29-31 NN denotes ES
T11481 32-37 NNS denotes cells
T11483 38-42 IN denotes from
T11484 43-54 NNS denotes blastocysts
T11485 54-198 sentence denotes Esg1+/-or ESG1-/- mutant female mice were injected with Tamoxifen (10 μg) and Depo-provera (1 mg) subcutaneously on the third day of pregnancy.
T11486 55-59 NN denotes Esg1
T11488 59-60 SYM denotes +
T11489 60-61 HYPH denotes /
T11490 61-62 SYM denotes -
T11491 62-64 CC denotes or
T11492 65-69 NN denotes ESG1
T11493 69-70 SYM denotes -
T11494 70-71 HYPH denotes /
T11495 71-72 SYM denotes -
T11496 73-79 NN denotes mutant
T11497 80-86 JJ denotes female
T11487 87-91 NNS denotes mice
T11499 92-96 VBD denotes were
T11498 97-105 VBN denotes injected
T11500 106-110 IN denotes with
T11501 111-120 NN denotes Tamoxifen
T11502 121-122 -LRB- denotes (
T11504 122-124 CD denotes 10
T11503 125-127 NN denotes μg
T11505 127-128 -RRB- denotes )
T11506 129-132 CC denotes and
T11507 133-137 NN denotes Depo
T11509 137-138 HYPH denotes -
T11508 138-145 NN denotes provera
T11510 146-147 -LRB- denotes (
T11512 147-148 CD denotes 1
T11511 149-151 NN denotes mg
T11513 151-152 -RRB- denotes )
T11514 153-167 RB denotes subcutaneously
T11515 168-170 IN denotes on
T11516 171-174 DT denotes the
T11518 175-180 JJ denotes third
T11517 181-184 NN denotes day
T11519 185-187 IN denotes of
T11520 188-197 NN denotes pregnancy
T11521 197-198 . denotes .
T11522 198-582 sentence denotes Four days later, embryos in diapause were flushed out of the uterus and cultured on STO feeder cells in four-well plates in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 20% Fetal Bovine Serum (Hyclone), 0.1 mM Non-Essential Amino Acids (Invitrogen), 2 mM L-glutamine (Invitrogen), 50 U/ml Penicillin-Streptomysin (Invitrogen), and 0.11 mM 2-mercaptoethanol (Invitrogen).
T11523 199-203 CD denotes Four
T11524 204-208 NNS denotes days
T11525 209-214 RB denotes later
T11527 214-216 , denotes ,
T11528 216-223 NNS denotes embryos
T11529 224-226 IN denotes in
T11530 227-235 NN denotes diapause
T11531 236-240 VBD denotes were
T11526 241-248 VBN denotes flushed
T11532 249-252 IN denotes out
T11533 253-255 IN denotes of
T11534 256-259 DT denotes the
T11535 260-266 NN denotes uterus
T11536 267-270 CC denotes and
T11537 271-279 VBN denotes cultured
T11538 280-282 IN denotes on
T11539 283-286 NN denotes STO
T11541 287-293 NN denotes feeder
T11540 294-299 NNS denotes cells
T11542 300-302 IN denotes in
T11543 303-307 CD denotes four
T11545 307-308 HYPH denotes -
T11544 308-312 NN denotes well
T11546 313-319 NNS denotes plates
T11547 320-322 IN denotes in
T11548 323-331 NNP denotes Dulbecco
T11550 331-333 POS denotes 's
T11551 334-342 VBN denotes Modified
T11552 343-348 NNP denotes Eagle
T11549 349-355 NNP denotes Medium
T11553 356-357 -LRB- denotes (
T11554 357-361 NN denotes DMEM
T11555 361-362 -RRB- denotes )
T11556 363-375 VBN denotes supplemented
T11557 376-380 IN denotes with
T11558 381-383 CD denotes 20
T11559 383-384 NN denotes %
T11561 385-390 JJ denotes Fetal
T11562 391-397 JJ denotes Bovine
T11560 398-403 NN denotes Serum
T11563 404-405 -LRB- denotes (
T11564 405-412 NN denotes Hyclone
T11565 412-413 -RRB- denotes )
T11566 413-415 , denotes ,
T11567 415-418 CD denotes 0.1
T11568 419-421 NN denotes mM
T11570 422-435 JJ denotes Non-Essential
T11571 436-441 NN denotes Amino
T11569 442-447 NNS denotes Acids
T11572 448-449 -LRB- denotes (
T11573 449-459 NNP denotes Invitrogen
T11574 459-460 -RRB- denotes )
T11575 460-462 , denotes ,
T11576 462-463 CD denotes 2
T11577 464-466 NN denotes mM
T11579 467-468 NN denotes L
T11580 468-469 HYPH denotes -
T11578 469-478 NN denotes glutamine
T11581 479-480 -LRB- denotes (
T11582 480-490 NNP denotes Invitrogen
T11583 490-491 -RRB- denotes )
T11584 491-493 , denotes ,
T11585 493-495 CD denotes 50
T11586 496-497 NN denotes U
T11588 497-498 SYM denotes /
T11589 498-500 NN denotes ml
T11590 501-511 NN denotes Penicillin
T11591 511-512 HYPH denotes -
T11587 512-524 NN denotes Streptomysin
T11592 525-526 -LRB- denotes (
T11593 526-536 NNP denotes Invitrogen
T11594 536-537 -RRB- denotes )
T11595 537-539 , denotes ,
T11596 539-542 CC denotes and
T11597 543-547 CD denotes 0.11
T11598 548-550 NN denotes mM
T11600 551-552 CD denotes 2
T11601 552-553 HYPH denotes -
T11599 553-568 NN denotes mercaptoethanol
T11602 569-570 -LRB- denotes (
T11603 570-580 NNP denotes Invitrogen
T11604 580-581 -RRB- denotes )
T11605 581-582 . denotes .
T11606 582-712 sentence denotes After six days, the central mass of each explant was harvested, rinsed in PBS, and placed in a drop of trypsin for a few minutes.
T11607 583-588 IN denotes After
T11609 589-592 CD denotes six
T11610 593-597 NNS denotes days
T11611 597-599 , denotes ,
T11612 599-602 DT denotes the
T11614 603-610 JJ denotes central
T11613 611-615 NN denotes mass
T11615 616-618 IN denotes of
T11616 619-623 DT denotes each
T11617 624-631 NN denotes explant
T11618 632-635 VBD denotes was
T11608 636-645 VBN denotes harvested
T11619 645-647 , denotes ,
T11620 647-653 VBN denotes rinsed
T11621 654-656 IN denotes in
T11622 657-660 NN denotes PBS
T11623 660-662 , denotes ,
T11624 662-665 CC denotes and
T11625 666-672 VBN denotes placed
T11626 673-675 IN denotes in
T11627 676-677 DT denotes a
T11628 678-682 NN denotes drop
T11629 683-685 IN denotes of
T11630 686-693 NN denotes trypsin
T11631 694-697 IN denotes for
T11632 698-699 DT denotes a
T11634 700-703 JJ denotes few
T11633 704-711 NNS denotes minutes
T11635 711-712 . denotes .
T11636 712-846 sentence denotes The cell mass was collected with a finely drawn-out Pasteur pipette preloaded with medium, ensuring minimal carryover of the trypsin.
T11637 713-716 DT denotes The
T11639 717-721 NN denotes cell
T11638 722-726 NN denotes mass
T11641 727-730 VBD denotes was
T11640 731-740 VBN denotes collected
T11642 741-745 IN denotes with
T11643 746-747 DT denotes a
T11645 748-754 RB denotes finely
T11646 755-760 VBN denotes drawn
T11647 760-761 HYPH denotes -
T11648 761-764 RP denotes out
T11649 765-772 NNP denotes Pasteur
T11644 773-780 NN denotes pipette
T11650 781-790 VBN denotes preloaded
T11651 791-795 IN denotes with
T11652 796-802 NN denotes medium
T11653 802-804 , denotes ,
T11654 804-812 VBG denotes ensuring
T11655 813-820 JJ denotes minimal
T11656 821-830 NN denotes carryover
T11657 831-833 IN denotes of
T11658 834-837 DT denotes the
T11659 838-845 NN denotes trypsin
T11660 845-846 . denotes .
T11661 846-929 sentence denotes The cells were gently transfered into a fresh well with 20% FBS-containing medium.
T11662 847-850 DT denotes The
T11663 851-856 NNS denotes cells
T11665 857-861 VBD denotes were
T11666 862-868 RB denotes gently
T11664 869-879 VBN denotes transfered
T11667 880-884 IN denotes into
T11668 885-886 DT denotes a
T11670 887-892 JJ denotes fresh
T11669 893-897 NN denotes well
T11671 898-902 IN denotes with
T11672 903-905 CD denotes 20
T11673 905-906 NN denotes %
T11675 907-910 NN denotes FBS
T11677 910-911 HYPH denotes -
T11676 911-921 VBG denotes containing
T11674 922-928 NN denotes medium
T11678 928-929 . denotes .
T11679 929-1061 sentence denotes The resulting primary ES cell colonies were individually passaged into wells of four-well plates containing STO feeder cell layers.
T11680 930-933 DT denotes The
T11682 934-943 VBG denotes resulting
T11683 944-951 JJ denotes primary
T11684 952-954 NN denotes ES
T11685 955-959 NN denotes cell
T11681 960-968 NNS denotes colonies
T11687 969-973 VBD denotes were
T11688 974-986 RB denotes individually
T11686 987-995 VBN denotes passaged
T11689 996-1000 IN denotes into
T11690 1001-1006 NNS denotes wells
T11691 1007-1009 IN denotes of
T11692 1010-1014 CD denotes four
T11694 1014-1015 HYPH denotes -
T11693 1015-1019 NN denotes well
T11695 1020-1026 NNS denotes plates
T11696 1027-1037 VBG denotes containing
T11697 1038-1041 NN denotes STO
T11699 1042-1048 NN denotes feeder
T11700 1049-1053 NN denotes cell
T11698 1054-1060 NNS denotes layers
T11701 1060-1061 . denotes .
T11702 1061-1134 sentence denotes Thereafter, cells were expanded by trypsinization of the entire culture.
T11703 1062-1072 RB denotes Thereafter
T11705 1072-1074 , denotes ,
T11706 1074-1079 NNS denotes cells
T11707 1080-1084 VBD denotes were
T11704 1085-1093 VBN denotes expanded
T11708 1094-1096 IN denotes by
T11709 1097-1111 NN denotes trypsinization
T11710 1112-1114 IN denotes of
T11711 1115-1118 DT denotes the
T11713 1119-1125 JJ denotes entire
T11712 1126-1133 NN denotes culture
T11714 1133-1134 . denotes .
R3168 T11477 T11476 prep of,Derivation
R3169 T11478 T11479 npadvmod ESG1,deficient
R3170 T11479 T11481 amod deficient,cells
R3171 T11480 T11479 punct -,deficient
R3172 T11481 T11477 pobj cells,of
R3173 T11482 T11481 compound ES,cells
R3174 T11483 T11476 prep from,Derivation
R3175 T11484 T11483 pobj blastocysts,from
R3176 T11486 T11487 nmod Esg1,mice
R3177 T11487 T11498 nsubjpass mice,injected
R3178 T11488 T11486 punct +,Esg1
R3179 T11489 T11486 punct /,Esg1
R3180 T11490 T11486 punct -,Esg1
R3181 T11491 T11486 cc or,Esg1
R3182 T11492 T11486 conj ESG1,Esg1
R3183 T11493 T11492 punct -,ESG1
R3184 T11494 T11492 punct /,ESG1
R3185 T11495 T11492 punct -,ESG1
R3186 T11496 T11487 nmod mutant,mice
R3187 T11497 T11487 amod female,mice
R3188 T11499 T11498 auxpass were,injected
R3189 T11500 T11498 prep with,injected
R3190 T11501 T11500 pobj Tamoxifen,with
R3191 T11502 T11503 punct (,μg
R3192 T11503 T11501 parataxis μg,Tamoxifen
R3193 T11504 T11503 nummod 10,μg
R3194 T11505 T11503 punct ),μg
R3195 T11506 T11501 cc and,Tamoxifen
R3196 T11507 T11508 compound Depo,provera
R3197 T11508 T11501 conj provera,Tamoxifen
R3198 T11509 T11508 punct -,provera
R3199 T11510 T11511 punct (,mg
R3200 T11511 T11508 parataxis mg,provera
R3201 T11512 T11511 nummod 1,mg
R3202 T11513 T11511 punct ),mg
R3203 T11514 T11498 advmod subcutaneously,injected
R3204 T11515 T11498 prep on,injected
R3205 T11516 T11517 det the,day
R3206 T11517 T11515 pobj day,on
R3207 T11518 T11517 amod third,day
R3208 T11519 T11517 prep of,day
R3209 T11520 T11519 pobj pregnancy,of
R3210 T11521 T11498 punct .,injected
R3211 T11523 T11524 nummod Four,days
R3212 T11524 T11525 npadvmod days,later
R3213 T11525 T11526 advmod later,flushed
R3214 T11527 T11526 punct ", ",flushed
R3215 T11528 T11526 nsubjpass embryos,flushed
R3216 T11529 T11528 prep in,embryos
R3217 T11530 T11529 pobj diapause,in
R3218 T11531 T11526 auxpass were,flushed
R3219 T11532 T11526 prep out,flushed
R3220 T11533 T11532 prep of,out
R3221 T11534 T11535 det the,uterus
R3222 T11535 T11533 pobj uterus,of
R3223 T11536 T11526 cc and,flushed
R3224 T11537 T11526 conj cultured,flushed
R3225 T11538 T11537 prep on,cultured
R3226 T11539 T11540 compound STO,cells
R3227 T11540 T11538 pobj cells,on
R3228 T11541 T11540 compound feeder,cells
R3229 T11542 T11537 prep in,cultured
R3230 T11543 T11544 nummod four,well
R3231 T11544 T11546 compound well,plates
R3232 T11545 T11544 punct -,well
R3233 T11546 T11542 pobj plates,in
R3234 T11547 T11537 prep in,cultured
R3235 T11548 T11549 poss Dulbecco,Medium
R3236 T11549 T11547 pobj Medium,in
R3237 T11550 T11548 case 's,Dulbecco
R3238 T11551 T11549 amod Modified,Medium
R3239 T11552 T11549 compound Eagle,Medium
R3240 T11553 T11549 punct (,Medium
R3241 T11554 T11549 appos DMEM,Medium
R3242 T11555 T11549 punct ),Medium
R3243 T11556 T11549 acl supplemented,Medium
R3244 T11557 T11556 prep with,supplemented
R3245 T11558 T11559 nummod 20,%
R3246 T11559 T11560 nmod %,Serum
R3247 T11560 T11557 pobj Serum,with
R3248 T11561 T11560 amod Fetal,Serum
R3249 T11562 T11560 amod Bovine,Serum
R3250 T11563 T11564 punct (,Hyclone
R3251 T11564 T11560 parataxis Hyclone,Serum
R3252 T11565 T11564 punct ),Hyclone
R3253 T11566 T11560 punct ", ",Serum
R3254 T11567 T11568 nummod 0.1,mM
R3255 T11568 T11569 nmod mM,Acids
R3256 T11569 T11560 conj Acids,Serum
R3257 T11570 T11569 amod Non-Essential,Acids
R3258 T11571 T11569 compound Amino,Acids
R3259 T11572 T11573 punct (,Invitrogen
R3260 T11573 T11569 parataxis Invitrogen,Acids
R3261 T11574 T11573 punct ),Invitrogen
R3262 T11575 T11569 punct ", ",Acids
R3263 T11576 T11577 nummod 2,mM
R3264 T11577 T11578 compound mM,glutamine
R3265 T11578 T11569 conj glutamine,Acids
R3266 T11579 T11578 compound L,glutamine
R3267 T11580 T11578 punct -,glutamine
R3268 T11581 T11582 punct (,Invitrogen
R3269 T11582 T11578 parataxis Invitrogen,glutamine
R3270 T11583 T11582 punct ),Invitrogen
R3271 T11584 T11578 punct ", ",glutamine
R3272 T11585 T11586 nummod 50,U
R3273 T11586 T11587 nmod U,Streptomysin
R3274 T11587 T11578 conj Streptomysin,glutamine
R3275 T11588 T11589 punct /,ml
R3276 T11589 T11586 prep ml,U
R3277 T11590 T11587 compound Penicillin,Streptomysin
R3278 T11591 T11587 punct -,Streptomysin
R3279 T11592 T11593 punct (,Invitrogen
R3280 T11593 T11587 parataxis Invitrogen,Streptomysin
R3281 T11594 T11593 punct ),Invitrogen
R3282 T11595 T11587 punct ", ",Streptomysin
R3283 T11596 T11587 cc and,Streptomysin
R3284 T11597 T11598 nummod 0.11,mM
R3285 T11598 T11599 nmod mM,mercaptoethanol
R3286 T11599 T11587 conj mercaptoethanol,Streptomysin
R3287 T11600 T11599 nummod 2,mercaptoethanol
R3288 T11601 T11599 punct -,mercaptoethanol
R3289 T11602 T11603 punct (,Invitrogen
R3290 T11603 T11599 parataxis Invitrogen,mercaptoethanol
R3291 T11604 T11603 punct ),Invitrogen
R3292 T11605 T11526 punct .,flushed
R3293 T11607 T11608 prep After,harvested
R3294 T11609 T11610 nummod six,days
R3295 T11610 T11607 pobj days,After
R3296 T11611 T11608 punct ", ",harvested
R3297 T11612 T11613 det the,mass
R3298 T11613 T11608 nsubjpass mass,harvested
R3299 T11614 T11613 amod central,mass
R3300 T11615 T11613 prep of,mass
R3301 T11616 T11617 det each,explant
R3302 T11617 T11615 pobj explant,of
R3303 T11618 T11608 auxpass was,harvested
R3304 T11619 T11608 punct ", ",harvested
R3305 T11620 T11608 conj rinsed,harvested
R3306 T11621 T11620 prep in,rinsed
R3307 T11622 T11621 pobj PBS,in
R3308 T11623 T11620 punct ", ",rinsed
R3309 T11624 T11620 cc and,rinsed
R3310 T11625 T11620 conj placed,rinsed
R3311 T11626 T11625 prep in,placed
R3312 T11627 T11628 det a,drop
R3313 T11628 T11626 pobj drop,in
R3314 T11629 T11628 prep of,drop
R3315 T11630 T11629 pobj trypsin,of
R3316 T11631 T11625 prep for,placed
R3317 T11632 T11633 det a,minutes
R3318 T11633 T11631 pobj minutes,for
R3319 T11634 T11633 amod few,minutes
R3320 T11635 T11608 punct .,harvested
R3321 T11637 T11638 det The,mass
R3322 T11638 T11640 nsubjpass mass,collected
R3323 T11639 T11638 compound cell,mass
R3324 T11641 T11640 auxpass was,collected
R3325 T11642 T11640 prep with,collected
R3326 T11643 T11644 det a,pipette
R3327 T11644 T11642 pobj pipette,with
R3328 T11645 T11646 advmod finely,drawn
R3329 T11646 T11644 amod drawn,pipette
R3330 T11647 T11646 punct -,drawn
R3331 T11648 T11646 prt out,drawn
R3332 T11649 T11644 compound Pasteur,pipette
R3333 T11650 T11644 acl preloaded,pipette
R3334 T11651 T11650 prep with,preloaded
R3335 T11652 T11651 pobj medium,with
R3336 T11653 T11640 punct ", ",collected
R3337 T11654 T11640 advcl ensuring,collected
R3338 T11655 T11656 amod minimal,carryover
R3339 T11656 T11654 dobj carryover,ensuring
R3340 T11657 T11656 prep of,carryover
R3341 T11658 T11659 det the,trypsin
R3342 T11659 T11657 pobj trypsin,of
R3343 T11660 T11640 punct .,collected
R3344 T11662 T11663 det The,cells
R3345 T11663 T11664 nsubjpass cells,transfered
R3346 T11665 T11664 auxpass were,transfered
R3347 T11666 T11664 advmod gently,transfered
R3348 T11667 T11664 prep into,transfered
R3349 T11668 T11669 det a,well
R3350 T11669 T11667 pobj well,into
R3351 T11670 T11669 amod fresh,well
R3352 T11671 T11664 prep with,transfered
R3353 T11672 T11673 nummod 20,%
R3354 T11673 T11674 nmod %,medium
R3355 T11674 T11671 pobj medium,with
R3356 T11675 T11676 npadvmod FBS,containing
R3357 T11676 T11674 amod containing,medium
R3358 T11677 T11676 punct -,containing
R3359 T11678 T11664 punct .,transfered
R3360 T11680 T11681 det The,colonies
R3361 T11681 T11686 nsubjpass colonies,passaged
R3362 T11682 T11681 amod resulting,colonies
R3363 T11683 T11681 amod primary,colonies
R3364 T11684 T11681 compound ES,colonies
R3365 T11685 T11681 compound cell,colonies
R3366 T11687 T11686 auxpass were,passaged
R3367 T11688 T11686 advmod individually,passaged
R3368 T11689 T11686 prep into,passaged
R3369 T11690 T11689 pobj wells,into
R3370 T11691 T11690 prep of,wells
R3371 T11692 T11693 nummod four,well
R3372 T11693 T11695 compound well,plates
R3373 T11694 T11693 punct -,well
R3374 T11695 T11691 pobj plates,of
R3375 T11696 T11690 acl containing,wells
R3376 T11697 T11698 compound STO,layers
R3377 T11698 T11696 dobj layers,containing
R3378 T11699 T11700 compound feeder,cell
R3379 T11700 T11698 compound cell,layers
R3380 T11701 T11686 punct .,passaged
R3381 T11703 T11704 advmod Thereafter,expanded
R3382 T11705 T11704 punct ", ",expanded
R3383 T11706 T11704 nsubjpass cells,expanded
R3384 T11707 T11704 auxpass were,expanded
R3385 T11708 T11704 prep by,expanded
R3386 T11709 T11708 pobj trypsinization,by
R3387 T11710 T11709 prep of,trypsinization
R3388 T11711 T11712 det the,culture
R3389 T11712 T11710 pobj culture,of
R3390 T11713 T11712 amod entire,culture
R3391 T11714 T11704 punct .,expanded