| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T10856 |
0-7 |
NNP |
denotes |
Western |
| T10857 |
8-12 |
NN |
denotes |
blot |
| T10858 |
12-217 |
sentence |
denotes |
After preparation of ES cell extracts with M-Per (Pierce), cellular proteins were separated on sodium dodecyl sulfate (SDS)-14% polyacrylamide gels and transferred to nitrocellulose membranes (Millipore). |
| T10859 |
13-18 |
IN |
denotes |
After |
| T10861 |
19-30 |
NN |
denotes |
preparation |
| T10862 |
31-33 |
IN |
denotes |
of |
| T10863 |
34-36 |
NN |
denotes |
ES |
| T10865 |
37-41 |
NN |
denotes |
cell |
| T10864 |
42-50 |
NNS |
denotes |
extracts |
| T10866 |
51-55 |
IN |
denotes |
with |
| T10867 |
56-57 |
NN |
denotes |
M |
| T10869 |
57-58 |
HYPH |
denotes |
- |
| T10868 |
58-61 |
NN |
denotes |
Per |
| T10870 |
62-63 |
-LRB- |
denotes |
( |
| T10871 |
63-69 |
NNP |
denotes |
Pierce |
| T10872 |
69-70 |
-RRB- |
denotes |
) |
| T10873 |
70-72 |
, |
denotes |
, |
| T10874 |
72-80 |
JJ |
denotes |
cellular |
| T10875 |
81-89 |
NN |
denotes |
proteins |
| T10876 |
90-94 |
VBD |
denotes |
were |
| T10860 |
95-104 |
VBN |
denotes |
separated |
| T10877 |
105-107 |
IN |
denotes |
on |
| T10878 |
108-114 |
NN |
denotes |
sodium |
| T10880 |
115-122 |
NN |
denotes |
dodecyl |
| T10879 |
123-130 |
NN |
denotes |
sulfate |
| T10882 |
131-132 |
-LRB- |
denotes |
( |
| T10883 |
132-135 |
NN |
denotes |
SDS |
| T10884 |
135-136 |
-RRB- |
denotes |
) |
| T10885 |
136-137 |
HYPH |
denotes |
- |
| T10886 |
137-139 |
CD |
denotes |
14 |
| T10887 |
139-140 |
NN |
denotes |
% |
| T10888 |
141-155 |
NN |
denotes |
polyacrylamide |
| T10881 |
156-160 |
NNS |
denotes |
gels |
| T10889 |
161-164 |
CC |
denotes |
and |
| T10890 |
165-176 |
VBN |
denotes |
transferred |
| T10891 |
177-179 |
IN |
denotes |
to |
| T10892 |
180-194 |
NN |
denotes |
nitrocellulose |
| T10893 |
195-204 |
NNS |
denotes |
membranes |
| T10894 |
205-206 |
-LRB- |
denotes |
( |
| T10895 |
206-215 |
NNP |
denotes |
Millipore |
| T10896 |
215-216 |
-RRB- |
denotes |
) |
| T10897 |
216-217 |
. |
denotes |
. |
| T10898 |
217-414 |
sentence |
denotes |
Membranes were incubated with anti-ESG1 (1/500 dilution), anti-Oct3/4 (1/500; Santa Cruz Biotechnology), anti-CDK4 (1/200; Santa Cruz Biotechnology), and anti-GFP (1/1000; MBL) primary antibodies. |
| T10899 |
218-227 |
NNS |
denotes |
Membranes |
| T10901 |
228-232 |
VBD |
denotes |
were |
| T10900 |
233-242 |
VBN |
denotes |
incubated |
| T10902 |
243-247 |
IN |
denotes |
with |
| T10903 |
248-257 |
NN |
denotes |
anti-ESG1 |
| T10905 |
258-259 |
-LRB- |
denotes |
( |
| T10907 |
259-260 |
CD |
denotes |
1 |
| T10909 |
260-261 |
SYM |
denotes |
/ |
| T10908 |
261-264 |
CD |
denotes |
500 |
| T10906 |
265-273 |
NN |
denotes |
dilution |
| T10910 |
273-274 |
-RRB- |
denotes |
) |
| T10911 |
274-276 |
, |
denotes |
, |
| T10912 |
276-285 |
NN |
denotes |
anti-Oct3 |
| T10913 |
285-286 |
HYPH |
denotes |
/ |
| T10914 |
286-287 |
CD |
denotes |
4 |
| T10915 |
288-289 |
-LRB- |
denotes |
( |
| T10917 |
289-290 |
CD |
denotes |
1 |
| T10919 |
290-291 |
SYM |
denotes |
/ |
| T10918 |
291-294 |
CD |
denotes |
500 |
| T10920 |
294-295 |
: |
denotes |
; |
| T10921 |
296-301 |
NNP |
denotes |
Santa |
| T10922 |
302-306 |
NNP |
denotes |
Cruz |
| T10916 |
307-320 |
NNP |
denotes |
Biotechnology |
| T10923 |
320-321 |
-RRB- |
denotes |
) |
| T10924 |
321-323 |
, |
denotes |
, |
| T10925 |
323-332 |
NN |
denotes |
anti-CDK4 |
| T10926 |
333-334 |
-LRB- |
denotes |
( |
| T10928 |
334-335 |
CD |
denotes |
1 |
| T10930 |
335-336 |
SYM |
denotes |
/ |
| T10929 |
336-339 |
CD |
denotes |
200 |
| T10931 |
339-340 |
: |
denotes |
; |
| T10932 |
341-346 |
NNP |
denotes |
Santa |
| T10933 |
347-351 |
NNP |
denotes |
Cruz |
| T10927 |
352-365 |
NNP |
denotes |
Biotechnology |
| T10934 |
365-366 |
-RRB- |
denotes |
) |
| T10935 |
366-368 |
, |
denotes |
, |
| T10936 |
368-371 |
CC |
denotes |
and |
| T10937 |
372-380 |
NN |
denotes |
anti-GFP |
| T10938 |
381-382 |
-LRB- |
denotes |
( |
| T10940 |
382-383 |
CD |
denotes |
1 |
| T10942 |
383-384 |
: |
denotes |
/ |
| T10941 |
384-388 |
CD |
denotes |
1000 |
| T10943 |
388-389 |
: |
denotes |
; |
| T10939 |
390-393 |
NN |
denotes |
MBL |
| T10944 |
393-394 |
-RRB- |
denotes |
) |
| T10945 |
395-402 |
JJ |
denotes |
primary |
| T10904 |
403-413 |
NNS |
denotes |
antibodies |
| T10946 |
413-414 |
. |
denotes |
. |
| T10947 |
414-554 |
sentence |
denotes |
Horseradish peroxidase-conjugated anti-rabbit and anti-mouse immunoglobulins (1/5000; Cell Signaling) were used to detect antibody binding. |
| T10948 |
415-426 |
NN |
denotes |
Horseradish |
| T10949 |
427-437 |
NN |
denotes |
peroxidase |
| T10951 |
437-438 |
HYPH |
denotes |
- |
| T10950 |
438-448 |
VBN |
denotes |
conjugated |
| T10953 |
449-460 |
JJ |
denotes |
anti-rabbit |
| T10954 |
461-464 |
CC |
denotes |
and |
| T10955 |
465-475 |
JJ |
denotes |
anti-mouse |
| T10952 |
476-491 |
NNS |
denotes |
immunoglobulins |
| T10957 |
492-493 |
-LRB- |
denotes |
( |
| T10959 |
493-494 |
CD |
denotes |
1 |
| T10961 |
494-495 |
SYM |
denotes |
/ |
| T10960 |
495-499 |
CD |
denotes |
5000 |
| T10962 |
499-500 |
: |
denotes |
; |
| T10963 |
501-505 |
NN |
denotes |
Cell |
| T10958 |
506-515 |
NN |
denotes |
Signaling |
| T10964 |
515-516 |
-RRB- |
denotes |
) |
| T10965 |
517-521 |
VBD |
denotes |
were |
| T10956 |
522-526 |
VBN |
denotes |
used |
| T10966 |
527-529 |
TO |
denotes |
to |
| T10967 |
530-536 |
VB |
denotes |
detect |
| T10968 |
537-545 |
NN |
denotes |
antibody |
| T10969 |
546-553 |
NN |
denotes |
binding |
| T10970 |
553-554 |
. |
denotes |
. |
| T10971 |
554-641 |
sentence |
denotes |
We visualized bound antibody with an ECL Western Blotting Detection System (Amersham). |
| T10972 |
555-557 |
PRP |
denotes |
We |
| T10973 |
558-568 |
VBD |
denotes |
visualized |
| T10974 |
569-574 |
VBN |
denotes |
bound |
| T10975 |
575-583 |
NN |
denotes |
antibody |
| T10976 |
584-588 |
IN |
denotes |
with |
| T10977 |
589-591 |
DT |
denotes |
an |
| T10979 |
592-595 |
NNP |
denotes |
ECL |
| T10980 |
596-603 |
NNP |
denotes |
Western |
| T10981 |
604-612 |
NNP |
denotes |
Blotting |
| T10982 |
613-622 |
NNP |
denotes |
Detection |
| T10978 |
623-629 |
NNP |
denotes |
System |
| T10983 |
630-631 |
-LRB- |
denotes |
( |
| T10984 |
631-639 |
NNP |
denotes |
Amersham |
| T10985 |
639-640 |
-RRB- |
denotes |
) |
| T10986 |
640-641 |
. |
denotes |
. |
| R3044 |
T10856 |
T10857 |
compound |
Western,blot |
| R3045 |
T10859 |
T10860 |
prep |
After,separated |
| R3046 |
T10861 |
T10859 |
pobj |
preparation,After |
| R3047 |
T10862 |
T10861 |
prep |
of,preparation |
| R3048 |
T10863 |
T10864 |
compound |
ES,extracts |
| R3049 |
T10864 |
T10862 |
pobj |
extracts,of |
| R3050 |
T10865 |
T10864 |
compound |
cell,extracts |
| R3051 |
T10866 |
T10861 |
prep |
with,preparation |
| R3052 |
T10867 |
T10868 |
compound |
M,Per |
| R3053 |
T10868 |
T10866 |
pobj |
Per,with |
| R3054 |
T10869 |
T10868 |
punct |
-,Per |
| R3055 |
T10870 |
T10871 |
punct |
(,Pierce |
| R3056 |
T10871 |
T10868 |
parataxis |
Pierce,Per |
| R3057 |
T10872 |
T10871 |
punct |
),Pierce |
| R3058 |
T10873 |
T10860 |
punct |
", ",separated |
| R3059 |
T10874 |
T10875 |
amod |
cellular,proteins |
| R3060 |
T10875 |
T10860 |
nsubjpass |
proteins,separated |
| R3061 |
T10876 |
T10860 |
auxpass |
were,separated |
| R3062 |
T10877 |
T10860 |
prep |
on,separated |
| R3063 |
T10878 |
T10879 |
nmod |
sodium,sulfate |
| R3064 |
T10879 |
T10881 |
nmod |
sulfate,gels |
| R3065 |
T10880 |
T10879 |
nmod |
dodecyl,sulfate |
| R3066 |
T10881 |
T10877 |
pobj |
gels,on |
| R3067 |
T10882 |
T10879 |
punct |
(,sulfate |
| R3068 |
T10883 |
T10879 |
appos |
SDS,sulfate |
| R3069 |
T10884 |
T10881 |
punct |
),gels |
| R3070 |
T10885 |
T10881 |
punct |
-,gels |
| R3071 |
T10886 |
T10887 |
nummod |
14,% |
| R3072 |
T10887 |
T10881 |
compound |
%,gels |
| R3073 |
T10888 |
T10881 |
compound |
polyacrylamide,gels |
| R3074 |
T10889 |
T10860 |
cc |
and,separated |
| R3075 |
T10890 |
T10860 |
conj |
transferred,separated |
| R3076 |
T10891 |
T10890 |
prep |
to,transferred |
| R3077 |
T10892 |
T10893 |
compound |
nitrocellulose,membranes |
| R3078 |
T10893 |
T10891 |
pobj |
membranes,to |
| R3079 |
T10894 |
T10895 |
punct |
(,Millipore |
| R3080 |
T10895 |
T10890 |
parataxis |
Millipore,transferred |
| R3081 |
T10896 |
T10895 |
punct |
),Millipore |
| R3082 |
T10897 |
T10860 |
punct |
.,separated |
| R3083 |
T10899 |
T10900 |
nsubjpass |
Membranes,incubated |
| R3084 |
T10901 |
T10900 |
auxpass |
were,incubated |
| R3085 |
T10902 |
T10900 |
prep |
with,incubated |
| R3086 |
T10903 |
T10904 |
nmod |
anti-ESG1,antibodies |
| R3087 |
T10904 |
T10902 |
pobj |
antibodies,with |
| R3088 |
T10905 |
T10906 |
punct |
(,dilution |
| R3089 |
T10906 |
T10903 |
parataxis |
dilution,anti-ESG1 |
| R3090 |
T10907 |
T10908 |
quantmod |
1,500 |
| R3091 |
T10908 |
T10906 |
nummod |
500,dilution |
| R3092 |
T10909 |
T10908 |
punct |
/,500 |
| R3093 |
T10910 |
T10906 |
punct |
),dilution |
| R3094 |
T10911 |
T10903 |
punct |
", ",anti-ESG1 |
| R3095 |
T10912 |
T10903 |
conj |
anti-Oct3,anti-ESG1 |
| R3096 |
T10913 |
T10912 |
punct |
/,anti-Oct3 |
| R3097 |
T10914 |
T10912 |
nummod |
4,anti-Oct3 |
| R3098 |
T10915 |
T10916 |
punct |
(,Biotechnology |
| R3099 |
T10916 |
T10912 |
parataxis |
Biotechnology,anti-Oct3 |
| R3100 |
T10917 |
T10918 |
quantmod |
1,500 |
| R3101 |
T10918 |
T10916 |
dep |
500,Biotechnology |
| R3102 |
T10919 |
T10918 |
punct |
/,500 |
| R3103 |
T10920 |
T10916 |
punct |
;,Biotechnology |
| R3104 |
T10921 |
T10916 |
compound |
Santa,Biotechnology |
| R3105 |
T10922 |
T10916 |
compound |
Cruz,Biotechnology |
| R3106 |
T10923 |
T10916 |
punct |
),Biotechnology |
| R3107 |
T10924 |
T10912 |
punct |
", ",anti-Oct3 |
| R3108 |
T10925 |
T10912 |
conj |
anti-CDK4,anti-Oct3 |
| R3109 |
T10926 |
T10927 |
punct |
(,Biotechnology |
| R3110 |
T10927 |
T10925 |
parataxis |
Biotechnology,anti-CDK4 |
| R3111 |
T10928 |
T10929 |
quantmod |
1,200 |
| R3112 |
T10929 |
T10927 |
dep |
200,Biotechnology |
| R3113 |
T10930 |
T10929 |
punct |
/,200 |
| R3114 |
T10931 |
T10927 |
punct |
;,Biotechnology |
| R3115 |
T10932 |
T10927 |
compound |
Santa,Biotechnology |
| R3116 |
T10933 |
T10927 |
compound |
Cruz,Biotechnology |
| R3117 |
T10934 |
T10927 |
punct |
),Biotechnology |
| R3118 |
T10935 |
T10925 |
punct |
", ",anti-CDK4 |
| R3119 |
T10936 |
T10925 |
cc |
and,anti-CDK4 |
| R3120 |
T10937 |
T10925 |
conj |
anti-GFP,anti-CDK4 |
| R3121 |
T10938 |
T10939 |
punct |
(,MBL |
| R3122 |
T10939 |
T10937 |
parataxis |
MBL,anti-GFP |
| R3123 |
T10940 |
T10941 |
quantmod |
1,1000 |
| R3124 |
T10941 |
T10939 |
dep |
1000,MBL |
| R3125 |
T10942 |
T10941 |
punct |
/,1000 |
| R3126 |
T10943 |
T10939 |
punct |
;,MBL |
| R3127 |
T10944 |
T10939 |
punct |
),MBL |
| R3128 |
T10945 |
T10904 |
amod |
primary,antibodies |
| R3129 |
T10946 |
T10900 |
punct |
.,incubated |
| R3130 |
T10948 |
T10949 |
compound |
Horseradish,peroxidase |
| R3131 |
T10949 |
T10950 |
npadvmod |
peroxidase,conjugated |
| R3132 |
T10950 |
T10952 |
amod |
conjugated,immunoglobulins |
| R3133 |
T10951 |
T10950 |
punct |
-,conjugated |
| R3134 |
T10952 |
T10956 |
nsubjpass |
immunoglobulins,used |
| R3135 |
T10953 |
T10952 |
amod |
anti-rabbit,immunoglobulins |
| R3136 |
T10954 |
T10953 |
cc |
and,anti-rabbit |
| R3137 |
T10955 |
T10953 |
conj |
anti-mouse,anti-rabbit |
| R3138 |
T10957 |
T10958 |
punct |
(,Signaling |
| R3139 |
T10958 |
T10952 |
parataxis |
Signaling,immunoglobulins |
| R3140 |
T10959 |
T10960 |
quantmod |
1,5000 |
| R3141 |
T10960 |
T10958 |
dep |
5000,Signaling |
| R3142 |
T10961 |
T10960 |
punct |
/,5000 |
| R3143 |
T10962 |
T10958 |
punct |
;,Signaling |
| R3144 |
T10963 |
T10958 |
compound |
Cell,Signaling |
| R3145 |
T10964 |
T10958 |
punct |
),Signaling |
| R3146 |
T10965 |
T10956 |
auxpass |
were,used |
| R3147 |
T10966 |
T10967 |
aux |
to,detect |
| R3148 |
T10967 |
T10956 |
advcl |
detect,used |
| R3149 |
T10968 |
T10969 |
compound |
antibody,binding |
| R3150 |
T10969 |
T10967 |
dobj |
binding,detect |
| R3151 |
T10970 |
T10956 |
punct |
.,used |
| R3152 |
T10972 |
T10973 |
nsubj |
We,visualized |
| R3153 |
T10974 |
T10975 |
amod |
bound,antibody |
| R3154 |
T10975 |
T10973 |
dobj |
antibody,visualized |
| R3155 |
T10976 |
T10973 |
prep |
with,visualized |
| R3156 |
T10977 |
T10978 |
det |
an,System |
| R3157 |
T10978 |
T10976 |
pobj |
System,with |
| R3158 |
T10979 |
T10978 |
compound |
ECL,System |
| R3159 |
T10980 |
T10981 |
compound |
Western,Blotting |
| R3160 |
T10981 |
T10978 |
compound |
Blotting,System |
| R3161 |
T10982 |
T10978 |
compound |
Detection,System |
| R3162 |
T10983 |
T10984 |
punct |
(,Amersham |
| R3163 |
T10984 |
T10978 |
parataxis |
Amersham,System |
| R3164 |
T10985 |
T10984 |
punct |
),Amersham |
| R3165 |
T10986 |
T10973 |
punct |
.,visualized |
