PMC:138691 JSON TXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T36	0-5	NN	denotes	BRCA2
T37	0-34	sentence	denotes	BRCA2 and homologous recombination
T38	6-9	CC	denotes	and
T39	10-20	JJ	denotes	homologous
T40	21-34	NN	denotes	recombination
T41	34-35	sentence	denotes
T43	45-48	CD	denotes	Two
T42	45-166	sentence	denotes	Two recent papers provide new evidence relevant to the role of the breast cancer susceptibility gene BRCA2 in DNA repair.
T45	49-55	JJ	denotes	recent
T44	56-62	NNS	denotes	papers
T46	63-70	VBP	denotes	provide
T47	71-74	JJ	denotes	new
T48	75-83	NN	denotes	evidence
T49	84-92	JJ	denotes	relevant
T50	93-95	IN	denotes	to
T51	96-99	DT	denotes	the
T52	100-104	NN	denotes	role
T53	105-107	IN	denotes	of
T54	108-111	DT	denotes	the
T56	112-118	NN	denotes	breast
T57	119-125	NN	denotes	cancer
T58	126-140	NN	denotes	susceptibility
T55	141-145	NN	denotes	gene
T59	146-151	NN	denotes	BRCA2
T60	152-154	IN	denotes	in
T61	155-158	NN	denotes	DNA
T62	159-165	NN	denotes	repair
T63	165-166	.	denotes	.
T64	166-313	sentence	denotes	Moynahan et al provide genetic data indicating a requirement for BRCA2 in homology-dependent (recombinational) repair of DNA double-strand breaks.
T65	167-175	NNP	denotes	Moynahan
T67	176-178	FW	denotes	et
T68	179-181	FW	denotes	al
T66	182-189	VBP	denotes	provide
T69	190-197	JJ	denotes	genetic
T70	198-202	NNS	denotes	data
T71	203-213	VBG	denotes	indicating
T72	214-215	DT	denotes	a
T73	216-227	NN	denotes	requirement
T74	228-231	IN	denotes	for
T75	232-237	NN	denotes	BRCA2
T76	238-240	IN	denotes	in
T77	241-249	JJ	denotes	homology
T79	249-250	HYPH	denotes	-
T78	250-259	JJ	denotes	dependent
T81	260-261	-LRB-	denotes	(
T80	261-276	JJ	denotes	recombinational
T83	276-277	-RRB-	denotes	)
T82	278-284	NN	denotes	repair
T84	285-287	IN	denotes	of
T85	288-291	NN	denotes	DNA
T87	292-298	JJ	denotes	double
T89	298-299	HYPH	denotes	-
T88	299-305	NN	denotes	strand
T86	306-312	NNS	denotes	breaks
T90	312-313	.	denotes	.
T91	313-450	sentence	denotes	The second paper, by Davies et al, begins to address the mechanism through which BRCA2 makes its contribution to recombinational repair.
T92	314-317	DT	denotes	The
T94	318-324	JJ	denotes	second
T93	325-330	NN	denotes	paper
T96	330-332	,	denotes	,
T97	332-334	IN	denotes	by
T98	335-341	NNP	denotes	Davies
T99	342-344	FW	denotes	et
T100	345-347	FW	denotes	al
T101	347-349	,	denotes	,
T95	349-355	VBZ	denotes	begins
T102	356-358	TO	denotes	to
T103	359-366	VB	denotes	address
T104	367-370	DT	denotes	the
T105	371-380	NN	denotes	mechanism
T106	381-388	IN	denotes	through
T108	389-394	WDT	denotes	which
T109	395-400	NN	denotes	BRCA2
T107	401-406	VBZ	denotes	makes
T110	407-410	PRP$	denotes	its
T111	411-423	NN	denotes	contribution
T112	424-426	IN	denotes	to
T113	427-442	JJ	denotes	recombinational
T114	443-449	NN	denotes	repair
T115	449-450	.	denotes	.
T116	450-563	sentence	denotes	BRCA2 appears to function in recombination via interactions with the major eukaryotic recombinase RAD51 [1,2,3].
T117	451-456	NN	denotes	BRCA2
T118	457-464	VBZ	denotes	appears
T119	465-467	TO	denotes	to
T120	468-476	VB	denotes	function
T121	477-479	IN	denotes	in
T122	480-493	NN	denotes	recombination
T123	494-497	IN	denotes	via
T124	498-510	NNS	denotes	interactions
T125	511-515	IN	denotes	with
T126	516-519	DT	denotes	the
T128	520-525	JJ	denotes	major
T129	526-536	JJ	denotes	eukaryotic
T127	537-548	NN	denotes	recombinase
T130	549-554	NN	denotes	RAD51
T131	555-556	-LRB-	denotes	[
T133	556-557	CD	denotes	1
T134	557-558	,	denotes	,
T135	558-559	CD	denotes	2
T136	559-560	,	denotes	,
T132	560-561	CD	denotes	3
T137	561-562	-RRB-	denotes	]
T138	562-563	.	denotes	.
T139	563-763	sentence	denotes	We briefly review the context in which the two studies were carried out, we comment on the results presented, and we discuss models designed to account for the role of BRCA2 in RAD51–mediated repair.
T140	564-566	PRP	denotes	We
T142	567-574	RB	denotes	briefly
T141	575-581	VBP	denotes	review
T143	582-585	DT	denotes	the
T144	586-593	NN	denotes	context
T145	594-596	IN	denotes	in
T147	597-602	WDT	denotes	which
T148	603-606	DT	denotes	the
T150	607-610	CD	denotes	two
T149	611-618	NNS	denotes	studies
T151	619-623	VBD	denotes	were
T146	624-631	VBN	denotes	carried
T152	632-635	RP	denotes	out
T153	635-637	,	denotes	,
T154	637-639	PRP	denotes	we
T155	640-647	VBP	denotes	comment
T156	648-650	IN	denotes	on
T157	651-654	DT	denotes	the
T158	655-662	NNS	denotes	results
T159	663-672	VBN	denotes	presented
T160	672-674	,	denotes	,
T161	674-677	CC	denotes	and
T162	678-680	PRP	denotes	we
T163	681-688	VBP	denotes	discuss
T164	689-695	NNS	denotes	models
T165	696-704	VBN	denotes	designed
T166	705-707	TO	denotes	to
T167	708-715	VB	denotes	account
T168	716-719	IN	denotes	for
T169	720-723	DT	denotes	the
T170	724-728	NN	denotes	role
T171	729-731	IN	denotes	of
T172	732-737	NN	denotes	BRCA2
T173	738-740	IN	denotes	in
T174	741-746	NN	denotes	RAD51
T176	746-747	HYPH	denotes	–
T175	747-755	VBN	denotes	mediated
T177	756-762	NN	denotes	repair
T178	762-763	.	denotes	.
T227	771-776	NN	denotes	BRCA2
T228	777-780	VBD	denotes	was
T229	781-784	DT	denotes	the
T231	785-791	JJ	denotes	second
T232	792-798	NN	denotes	breast
T233	799-805	NN	denotes	cancer
T234	806-820	NN	denotes	susceptibility
T230	821-825	NN	denotes	gene
T235	826-828	TO	denotes	to
T237	829-831	VB	denotes	be
T236	832-842	VBN	denotes	discovered
T238	842-844	,	denotes	,
T239	844-847	CC	denotes	and
T240	848-851	VBD	denotes	was
T241	852-860	VBN	denotes	isolated
T242	861-868	IN	denotes	through
T243	869-879	JJ	denotes	positional
T244	880-887	NN	denotes	cloning
T245	888-893	VBG	denotes	using
T246	894-898	NNS	denotes	data
T247	899-903	IN	denotes	from
T248	904-912	NNS	denotes	families
T249	913-917	IN	denotes	with
T250	918-927	JJ	denotes	inherited
T252	928-934	NN	denotes	breast
T251	935-941	NN	denotes	cancer
T253	942-943	-LRB-	denotes	[
T254	943-944	CD	denotes	4
T255	944-945	-RRB-	denotes	]
T256	945-946	.	denotes	.
T257	946-1083	sentence	denotes	Cells with mutant BRCA2 protein are, like many cancer cells, genetically unstable and accumulate gross chromosomal rearrangements [5,6].
T258	947-952	NNS	denotes	Cells
T260	953-957	IN	denotes	with
T261	958-964	JJ	denotes	mutant
T263	965-970	NN	denotes	BRCA2
T262	971-978	NN	denotes	protein
T259	979-982	VBP	denotes	are
T264	982-984	,	denotes	,
T265	984-988	IN	denotes	like
T266	989-993	JJ	denotes	many
T268	994-1000	NN	denotes	cancer
T267	1001-1006	NNS	denotes	cells
T269	1006-1008	,	denotes	,
T270	1008-1019	RB	denotes	genetically
T271	1020-1028	JJ	denotes	unstable
T272	1029-1032	CC	denotes	and
T273	1033-1043	VBP	denotes	accumulate
T274	1044-1049	JJ	denotes	gross
T276	1050-1061	JJ	denotes	chromosomal
T275	1062-1076	NNS	denotes	rearrangements
T277	1077-1078	-LRB-	denotes	[
T279	1078-1079	CD	denotes	5
T280	1079-1080	,	denotes	,
T278	1080-1081	CD	denotes	6
T281	1081-1082	-RRB-	denotes	]
T282	1082-1083	.	denotes	.
T283	1083-1330	sentence	denotes	The sequence of this large protein (3418 amino acids) offers very little clue to its function, although there are eight repeated segments (termed BRC repeats) in the middle of the protein that are highly conserved among mammalian orthologs [7,8].
T284	1084-1087	DT	denotes	The
T285	1088-1096	NN	denotes	sequence
T287	1097-1099	IN	denotes	of
T288	1100-1104	DT	denotes	this
T290	1105-1110	JJ	denotes	large
T289	1111-1118	NN	denotes	protein
T291	1119-1120	-LRB-	denotes	(
T292	1120-1124	CD	denotes	3418
T294	1125-1130	NN	denotes	amino
T293	1131-1136	NNS	denotes	acids
T295	1136-1137	-RRB-	denotes	)
T286	1138-1144	VBZ	denotes	offers
T296	1145-1149	RB	denotes	very
T298	1150-1156	JJ	denotes	little
T297	1157-1161	NN	denotes	clue
T299	1162-1164	IN	denotes	to
T300	1165-1168	PRP$	denotes	its
T301	1169-1177	NN	denotes	function
T302	1177-1179	,	denotes	,
T303	1179-1187	IN	denotes	although
T305	1188-1193	EX	denotes	there
T304	1194-1197	VBP	denotes	are
T306	1198-1203	CD	denotes	eight
T308	1204-1212	JJ	denotes	repeated
T307	1213-1221	NNS	denotes	segments
T309	1222-1223	-LRB-	denotes	(
T310	1223-1229	VBN	denotes	termed
T311	1230-1233	NN	denotes	BRC
T312	1234-1241	NNS	denotes	repeats
T313	1241-1242	-RRB-	denotes	)
T314	1243-1245	IN	denotes	in
T315	1246-1249	DT	denotes	the
T316	1250-1256	NN	denotes	middle
T317	1257-1259	IN	denotes	of
T318	1260-1263	DT	denotes	the
T319	1264-1271	NN	denotes	protein
T320	1272-1276	WDT	denotes	that
T321	1277-1280	VBP	denotes	are
T322	1281-1287	RB	denotes	highly
T323	1288-1297	VBN	denotes	conserved
T324	1298-1303	IN	denotes	among
T325	1304-1313	JJ	denotes	mammalian
T326	1314-1323	NNS	denotes	orthologs
T327	1324-1325	-LRB-	denotes	[
T329	1325-1326	CD	denotes	7
T330	1326-1327	,	denotes	,
T328	1327-1328	CD	denotes	8
T331	1328-1329	-RRB-	denotes	]
T332	1329-1330	.	denotes	.
T437	1382-1391	VBG	denotes	Following
T439	1392-1395	DT	denotes	the
T441	1396-1404	NN	denotes	landmark
T440	1405-1414	NN	denotes	discovery
T442	1415-1417	IN	denotes	by
T443	1418-1424	NNP	denotes	Scully
T444	1425-1427	FW	denotes	et
T445	1428-1430	FW	denotes	al
T446	1431-1435	IN	denotes	that
T448	1436-1439	DT	denotes	the
T450	1440-1450	JJ	denotes	homologous
T451	1451-1462	NN	denotes	recombinase
T449	1463-1468	NN	denotes	RAD51
T447	1469-1480	VBZ	denotes	colocalizes
T452	1481-1483	IN	denotes	at
T453	1484-1494	JJ	denotes	subnuclear
T454	1495-1500	NNS	denotes	sites
T455	1501-1505	IN	denotes	with
T456	1506-1511	NN	denotes	BRCA1
T457	1512-1513	-LRB-	denotes	[
T458	1513-1514	CD	denotes	9
T459	1514-1515	-RRB-	denotes	]
T460	1515-1517	,	denotes	,
T461	1517-1518	DT	denotes	a
T462	1519-1525	NN	denotes	number
T463	1526-1528	IN	denotes	of
T464	1529-1539	JJ	denotes	additional
T465	1540-1547	NNS	denotes	results
T466	1548-1552	VBP	denotes	have
T438	1553-1561	VBN	denotes	provided
T467	1562-1570	NN	denotes	evidence
T468	1571-1575	IN	denotes	that
T470	1576-1580	CC	denotes	both
T471	1581-1586	NN	denotes	BRCA1
T472	1587-1590	CC	denotes	and
T473	1591-1596	NN	denotes	BRCA2
T474	1597-1600	VBP	denotes	are
T469	1601-1609	VBN	denotes	involved
T475	1610-1612	IN	denotes	in
T476	1613-1628	JJ	denotes	recombinational
T477	1629-1635	NN	denotes	repair
T478	1636-1638	IN	denotes	of
T479	1639-1642	NN	denotes	DNA
T480	1643-1649	NN	denotes	damage
T481	1649-1650	.	denotes	.
T482	1650-1765	sentence	denotes	BRCA1 and BRCA2 form discrete nuclear foci during the S phase and after exposure to DNA damaging agents [9,10,11].
T483	1651-1656	NN	denotes	BRCA1
T485	1657-1660	CC	denotes	and
T486	1661-1666	NN	denotes	BRCA2
T484	1667-1671	VBP	denotes	form
T487	1672-1680	JJ	denotes	discrete
T489	1681-1688	JJ	denotes	nuclear
T488	1689-1693	NNS	denotes	foci
T490	1694-1700	IN	denotes	during
T491	1701-1704	DT	denotes	the
T493	1705-1706	NN	denotes	S
T492	1707-1712	NN	denotes	phase
T494	1713-1716	CC	denotes	and
T495	1717-1722	IN	denotes	after
T496	1723-1731	NN	denotes	exposure
T497	1732-1734	IN	denotes	to
T498	1735-1738	NN	denotes	DNA
T499	1739-1747	VBG	denotes	damaging
T500	1748-1754	NNS	denotes	agents
T501	1755-1756	-LRB-	denotes	[
T503	1756-1757	CD	denotes	9
T504	1757-1758	,	denotes	,
T505	1758-1760	CD	denotes	10
T506	1760-1761	,	denotes	,
T502	1761-1763	CD	denotes	11
T507	1763-1764	-RRB-	denotes	]
T508	1764-1765	.	denotes	.
T509	1765-1855	sentence	denotes	These foci are probably sites of repair of spontaneous and induced DNA damage [12,13,14].
T510	1766-1771	DT	denotes	These
T511	1772-1776	NNS	denotes	foci
T512	1777-1780	VBP	denotes	are
T513	1781-1789	RB	denotes	probably
T514	1790-1795	NNS	denotes	sites
T515	1796-1798	IN	denotes	of
T516	1799-1805	NN	denotes	repair
T517	1806-1808	IN	denotes	of
T518	1809-1820	JJ	denotes	spontaneous
T520	1821-1824	CC	denotes	and
T521	1825-1832	JJ	denotes	induced
T522	1833-1836	NN	denotes	DNA
T519	1837-1843	NN	denotes	damage
T523	1844-1845	-LRB-	denotes	[
T525	1845-1847	CD	denotes	12
T526	1847-1848	,	denotes	,
T527	1848-1850	CD	denotes	13
T528	1850-1851	,	denotes	,
T524	1851-1853	CD	denotes	14
T529	1853-1854	-RRB-	denotes	]
T530	1854-1855	.	denotes	.
T531	1855-2053	sentence	denotes	Cell lines defective in either BRCA1 or BRCA2 are sensitive to damaging agents that form double-strand breaks (DSBs), as are other cell lines defective in recombinational repair (reviewed in [15]).
T532	1856-1860	NN	denotes	Cell
T533	1861-1866	NNS	denotes	lines
T535	1867-1876	JJ	denotes	defective
T536	1877-1879	IN	denotes	in
T537	1880-1886	CC	denotes	either
T538	1887-1892	NN	denotes	BRCA1
T539	1893-1895	CC	denotes	or
T540	1896-1901	NN	denotes	BRCA2
T534	1902-1905	VBP	denotes	are
T541	1906-1915	JJ	denotes	sensitive
T542	1916-1918	IN	denotes	to
T543	1919-1927	VBG	denotes	damaging
T544	1928-1934	NNS	denotes	agents
T545	1935-1939	WDT	denotes	that
T546	1940-1944	VBP	denotes	form
T547	1945-1951	JJ	denotes	double
T549	1951-1952	HYPH	denotes	-
T548	1952-1958	JJ	denotes	strand
T550	1959-1965	NNS	denotes	breaks
T551	1966-1967	-LRB-	denotes	(
T552	1967-1971	NNS	denotes	DSBs
T553	1971-1972	-RRB-	denotes	)
T554	1972-1974	,	denotes	,
T555	1974-1976	IN	denotes	as
T556	1977-1980	VBP	denotes	are
T557	1981-1986	JJ	denotes	other
T559	1987-1991	NN	denotes	cell
T558	1992-1997	NNS	denotes	lines
T560	1998-2007	JJ	denotes	defective
T561	2008-2010	IN	denotes	in
T562	2011-2026	JJ	denotes	recombinational
T563	2027-2033	NN	denotes	repair
T564	2034-2035	-LRB-	denotes	(
T565	2035-2043	VBN	denotes	reviewed
T566	2044-2046	IN	denotes	in
T567	2047-2048	-LRB-	denotes	[
T568	2048-2050	CD	denotes	15
T569	2050-2051	-RRB-	denotes	]
T570	2051-2052	-RRB-	denotes	)
T571	2052-2053	.	denotes	.
T572	2053-2147	sentence	denotes	BRCA2 interacts with the RAD51 recombinase via direct protein-protein contacts [16,17,18,19].
T573	2054-2059	NN	denotes	BRCA2
T574	2060-2069	VBZ	denotes	interacts
T575	2070-2074	IN	denotes	with
T576	2075-2078	DT	denotes	the
T578	2079-2084	NN	denotes	RAD51
T577	2085-2096	NN	denotes	recombinase
T579	2097-2100	IN	denotes	via
T580	2101-2107	JJ	denotes	direct
T582	2108-2115	NN	denotes	protein
T584	2115-2116	HYPH	denotes	-
T583	2116-2123	NN	denotes	protein
T581	2124-2132	NNS	denotes	contacts
T585	2133-2134	-LRB-	denotes	[
T587	2134-2136	CD	denotes	16
T588	2136-2137	,	denotes	,
T589	2137-2139	CD	denotes	17
T590	2139-2140	,	denotes	,
T591	2140-2142	CD	denotes	18
T592	2142-2143	,	denotes	,
T586	2143-2145	CD	denotes	19
T593	2145-2146	-RRB-	denotes	]
T594	2146-2147	.	denotes	.
T595	2147-2282	sentence	denotes	Biochemical analysis also showed interaction between BRCA1 and RAD51, although these detected interactions may have been indirect [9].
T596	2148-2159	JJ	denotes	Biochemical
T597	2160-2168	NN	denotes	analysis
T599	2169-2173	RB	denotes	also
T598	2174-2180	VBD	denotes	showed
T600	2181-2192	NN	denotes	interaction
T601	2193-2200	IN	denotes	between
T602	2201-2206	NN	denotes	BRCA1
T603	2207-2210	CC	denotes	and
T604	2211-2216	NN	denotes	RAD51
T605	2216-2218	,	denotes	,
T606	2218-2226	IN	denotes	although
T608	2227-2232	DT	denotes	these
T610	2233-2241	VBN	denotes	detected
T609	2242-2254	NNS	denotes	interactions
T611	2255-2258	MD	denotes	may
T612	2259-2263	VB	denotes	have
T607	2264-2268	VBN	denotes	been
T613	2269-2277	JJ	denotes	indirect
T614	2278-2279	-LRB-	denotes	[
T615	2279-2280	CD	denotes	9
T616	2280-2281	-RRB-	denotes	]
T617	2281-2282	.	denotes	.
T618	2282-2353	sentence	denotes	The BRC repeats of BRCA2 are responsible for direct RAD51 interaction.
T619	2283-2286	DT	denotes	The
T621	2287-2290	NN	denotes	BRC
T620	2291-2298	NNS	denotes	repeats
T623	2299-2301	IN	denotes	of
T624	2302-2307	NN	denotes	BRCA2
T622	2308-2311	VBP	denotes	are
T625	2312-2323	JJ	denotes	responsible
T626	2324-2327	IN	denotes	for
T627	2328-2334	JJ	denotes	direct
T629	2335-2340	NN	denotes	RAD51
T628	2341-2352	NN	denotes	interaction
T630	2352-2353	.	denotes	.
T631	2353-2572	sentence	denotes	Cells lacking BRCA1/2 fail to form damage-induced subnuclear RAD51 foci with normal efficiency, suggesting that these proteins are required for the formation of recombinase complexes at the sites of DNA damage [20,21].
T632	2354-2359	NNS	denotes	Cells
T634	2360-2367	VBG	denotes	lacking
T635	2368-2373	NN	denotes	BRCA1
T637	2373-2374	HYPH	denotes	/
T636	2374-2375	NN	denotes	2
T633	2376-2380	VBP	denotes	fail
T638	2381-2383	TO	denotes	to
T639	2384-2388	VB	denotes	form
T640	2389-2395	NN	denotes	damage
T642	2395-2396	HYPH	denotes	-
T641	2396-2403	VBN	denotes	induced
T644	2404-2414	JJ	denotes	subnuclear
T643	2415-2420	NN	denotes	RAD51
T645	2421-2425	NNS	denotes	foci
T646	2426-2430	IN	denotes	with
T647	2431-2437	JJ	denotes	normal
T648	2438-2448	NN	denotes	efficiency
T649	2448-2450	,	denotes	,
T650	2450-2460	VBG	denotes	suggesting
T651	2461-2465	IN	denotes	that
T653	2466-2471	DT	denotes	these
T654	2472-2480	NN	denotes	proteins
T655	2481-2484	VBP	denotes	are
T652	2485-2493	VBN	denotes	required
T656	2494-2497	IN	denotes	for
T657	2498-2501	DT	denotes	the
T658	2502-2511	NN	denotes	formation
T659	2512-2514	IN	denotes	of
T660	2515-2526	NN	denotes	recombinase
T661	2527-2536	NNS	denotes	complexes
T662	2537-2539	IN	denotes	at
T663	2540-2543	DT	denotes	the
T664	2544-2549	NNS	denotes	sites
T665	2550-2552	IN	denotes	of
T666	2553-2556	NN	denotes	DNA
T667	2557-2563	NN	denotes	damage
T668	2564-2565	-LRB-	denotes	[
T670	2565-2567	CD	denotes	20
T671	2567-2568	,	denotes	,
T669	2568-2570	CD	denotes	21
T672	2570-2571	-RRB-	denotes	]
T673	2571-2572	.	denotes	.
T674	2572-2733	sentence	denotes	Finally, genetic measurements of recombination frequency have shown that Brca1-/- embryonic stem (ES) cells are deficient in recombinational DSB repair [22,23].
T675	2573-2580	RB	denotes	Finally
T677	2580-2582	,	denotes	,
T678	2582-2589	JJ	denotes	genetic
T679	2590-2602	NNS	denotes	measurements
T680	2603-2605	IN	denotes	of
T681	2606-2619	NN	denotes	recombination
T682	2620-2629	NN	denotes	frequency
T683	2630-2634	VBP	denotes	have
T676	2635-2640	VBN	denotes	shown
T684	2641-2645	IN	denotes	that
T686	2646-2651	NN	denotes	Brca1
T688	2651-2652	SYM	denotes	-
T689	2652-2653	HYPH	denotes	/
T690	2653-2654	SYM	denotes	-
T691	2655-2664	JJ	denotes	embryonic
T692	2665-2669	NN	denotes	stem
T693	2670-2671	-LRB-	denotes	(
T694	2671-2673	NN	denotes	ES
T695	2673-2674	-RRB-	denotes	)
T687	2675-2680	NNS	denotes	cells
T685	2681-2684	VBP	denotes	are
T696	2685-2694	JJ	denotes	deficient
T697	2695-2697	IN	denotes	in
T698	2698-2713	JJ	denotes	recombinational
T700	2714-2717	NN	denotes	DSB
T699	2718-2724	NN	denotes	repair
T701	2725-2726	-LRB-	denotes	[
T703	2726-2728	CD	denotes	22
T704	2728-2729	,	denotes	,
T702	2729-2731	CD	denotes	23
T705	2731-2732	-RRB-	denotes	]
T706	2732-2733	.	denotes	.
T707	2733-3028	sentence	denotes	The similarity between phenotypes associated with BRCA1 and BRCA2 deficiency, together with data showing a similar effect of DNA damage on distribution of BRCA1 and BRCA2 in repair-proficient cells, led to the hypothesis that BRCA2, like BRCA1, is required for efficient recombinational repair.
T708	2734-2737	DT	denotes	The
T709	2738-2748	NN	denotes	similarity
T711	2749-2756	IN	denotes	between
T712	2757-2767	NNS	denotes	phenotypes
T713	2768-2778	VBN	denotes	associated
T714	2779-2783	IN	denotes	with
T715	2784-2789	NN	denotes	BRCA1
T717	2790-2793	CC	denotes	and
T718	2794-2799	NN	denotes	BRCA2
T716	2800-2810	NN	denotes	deficiency
T719	2810-2812	,	denotes	,
T720	2812-2820	RB	denotes	together
T721	2821-2825	IN	denotes	with
T722	2826-2830	NNS	denotes	data
T723	2831-2838	VBG	denotes	showing
T724	2839-2840	DT	denotes	a
T726	2841-2848	JJ	denotes	similar
T725	2849-2855	NN	denotes	effect
T727	2856-2858	IN	denotes	of
T728	2859-2862	NN	denotes	DNA
T729	2863-2869	NN	denotes	damage
T730	2870-2872	IN	denotes	on
T731	2873-2885	NN	denotes	distribution
T732	2886-2888	IN	denotes	of
T733	2889-2894	NN	denotes	BRCA1
T734	2895-2898	CC	denotes	and
T735	2899-2904	NN	denotes	BRCA2
T736	2905-2907	IN	denotes	in
T737	2908-2914	NN	denotes	repair
T739	2914-2915	HYPH	denotes	-
T738	2915-2925	JJ	denotes	proficient
T740	2926-2931	NNS	denotes	cells
T741	2931-2933	,	denotes	,
T710	2933-2936	VBD	denotes	led
T742	2937-2939	IN	denotes	to
T743	2940-2943	DT	denotes	the
T744	2944-2954	NN	denotes	hypothesis
T745	2955-2959	IN	denotes	that
T747	2960-2965	NN	denotes	BRCA2
T748	2965-2967	,	denotes	,
T749	2967-2971	IN	denotes	like
T750	2972-2977	NN	denotes	BRCA1
T751	2977-2979	,	denotes	,
T752	2979-2981	VBZ	denotes	is
T746	2982-2990	VBN	denotes	required
T753	2991-2994	IN	denotes	for
T754	2995-3004	JJ	denotes	efficient
T756	3005-3020	JJ	denotes	recombinational
T755	3021-3027	NN	denotes	repair
T757	3027-3028	.	denotes	.
T758	3028-3109	sentence	denotes	The paper by Moynahan et al [24] provides important support for this hypothesis.
T759	3029-3032	DT	denotes	The
T760	3033-3038	NN	denotes	paper
T762	3039-3041	IN	denotes	by
T763	3042-3050	NNP	denotes	Moynahan
T764	3051-3053	FW	denotes	et
T765	3054-3056	FW	denotes	al
T766	3057-3058	-LRB-	denotes	[
T767	3058-3060	CD	denotes	24
T768	3060-3061	-RRB-	denotes	]
T761	3062-3070	VBZ	denotes	provides
T769	3071-3080	JJ	denotes	important
T770	3081-3088	NN	denotes	support
T771	3089-3092	IN	denotes	for
T772	3093-3097	DT	denotes	this
T773	3098-3108	NN	denotes	hypothesis
T774	3108-3109	.	denotes	.
T1002	3182-3188	NNP	denotes	Pierce
T1004	3189-3191	FW	denotes	et
T1005	3192-3194	FW	denotes	al
T1006	3195-3199	VBP	denotes	have
T1003	3200-3208	VBN	denotes	designed
T1007	3209-3210	DT	denotes	a
T1008	3211-3214	NN	denotes	set
T1009	3215-3217	IN	denotes	of
T1010	3218-3231	NN	denotes	recombination
T1011	3232-3242	NNS	denotes	substrates
T1012	3243-3246	IN	denotes	for
T1013	3247-3256	VBG	denotes	measuring
T1014	3257-3260	DT	denotes	the
T1015	3261-3266	NN	denotes	level
T1016	3267-3269	IN	denotes	of
T1017	3270-3280	JJ	denotes	homologous
T1018	3281-3294	NN	denotes	recombination
T1019	3295-3297	FW	denotes	in
T1020	3298-3302	FW	denotes	vivo
T1021	3303-3304	-LRB-	denotes	(
T1022	3304-3308	NN	denotes	Fig.
T1023	3309-3310	CD	denotes	1
T1024	3310-3311	-RRB-	denotes	)
T1025	3312-3313	-LRB-	denotes	[
T1026	3313-3315	CD	denotes	25
T1027	3315-3316	-RRB-	denotes	]
T1028	3316-3317	.	denotes	.
T1029	3317-3561	sentence	denotes	The DNA substrate contains a pair of mutated GFP genes (GFP encodes the easily detected green fluorescent protein), one of which contains a restriction site for I-SceI, a yeast intron encoded endonuclease with an 18 base pair recognition site.
T1030	3318-3321	DT	denotes	The
T1032	3322-3325	NN	denotes	DNA
T1031	3326-3335	NN	denotes	substrate
T1033	3336-3344	VBZ	denotes	contains
T1034	3345-3346	DT	denotes	a
T1035	3347-3351	NN	denotes	pair
T1036	3352-3354	IN	denotes	of
T1037	3355-3362	VBN	denotes	mutated
T1039	3363-3366	NN	denotes	GFP
T1038	3367-3372	NNS	denotes	genes
T1040	3373-3374	-LRB-	denotes	(
T1042	3374-3377	NN	denotes	GFP
T1041	3378-3385	VBZ	denotes	encodes
T1043	3386-3389	DT	denotes	the
T1045	3390-3396	RB	denotes	easily
T1046	3397-3405	VBN	denotes	detected
T1047	3406-3411	JJ	denotes	green
T1048	3412-3423	JJ	denotes	fluorescent
T1044	3424-3431	NN	denotes	protein
T1049	3431-3432	-RRB-	denotes	)
T1050	3432-3434	,	denotes	,
T1051	3434-3437	CD	denotes	one
T1053	3438-3440	IN	denotes	of
T1054	3441-3446	WDT	denotes	which
T1052	3447-3455	VBZ	denotes	contains
T1055	3456-3457	DT	denotes	a
T1057	3458-3469	NN	denotes	restriction
T1056	3470-3474	NN	denotes	site
T1058	3475-3478	IN	denotes	for
T1059	3479-3480	NN	denotes	I
T1061	3480-3481	HYPH	denotes	-
T1060	3481-3485	NN	denotes	SceI
T1062	3485-3487	,	denotes	,
T1063	3487-3488	DT	denotes	a
T1065	3489-3494	NN	denotes	yeast
T1066	3495-3501	NN	denotes	intron
T1067	3502-3509	VBN	denotes	encoded
T1064	3510-3522	NN	denotes	endonuclease
T1068	3523-3527	IN	denotes	with
T1069	3528-3530	DT	denotes	an
T1071	3531-3533	CD	denotes	18
T1073	3534-3538	NN	denotes	base
T1072	3539-3543	NN	denotes	pair
T1074	3544-3555	NN	denotes	recognition
T1070	3556-3560	NN	denotes	site
T1075	3560-3561	.	denotes	.
T1076	3561-3684	sentence	denotes	Transient transfection of an I-SceI expression vector results in the production of a DSB in the first mutated copy of GFP.
T1077	3562-3571	JJ	denotes	Transient
T1078	3572-3584	NN	denotes	transfection
T1080	3585-3587	IN	denotes	of
T1081	3588-3590	DT	denotes	an
T1083	3591-3592	NN	denotes	I
T1085	3592-3593	HYPH	denotes	-
T1084	3593-3597	NN	denotes	SceI
T1086	3598-3608	NN	denotes	expression
T1082	3609-3615	NN	denotes	vector
T1079	3616-3623	VBZ	denotes	results
T1087	3624-3626	IN	denotes	in
T1088	3627-3630	DT	denotes	the
T1089	3631-3641	NN	denotes	production
T1090	3642-3644	IN	denotes	of
T1091	3645-3646	DT	denotes	a
T1092	3647-3650	NN	denotes	DSB
T1093	3651-3653	IN	denotes	in
T1094	3654-3657	DT	denotes	the
T1096	3658-3663	JJ	denotes	first
T1097	3664-3671	VBN	denotes	mutated
T1095	3672-3676	NN	denotes	copy
T1098	3677-3679	IN	denotes	of
T1099	3680-3683	NN	denotes	GFP
T1100	3683-3684	.	denotes	.
T1101	3684-3868	sentence	denotes	One or both DNA ends formed by the break invade(s) the homologous sequence in the second mutant GFP copy, resulting in repair of the DSB via a homology-mediated gene conversion event.
T1102	3685-3688	CD	denotes	One
T1104	3689-3691	CC	denotes	or
T1105	3692-3696	CC	denotes	both
T1107	3697-3700	NN	denotes	DNA
T1106	3701-3705	NNS	denotes	ends
T1108	3706-3712	VBN	denotes	formed
T1109	3713-3715	IN	denotes	by
T1110	3716-3719	DT	denotes	the
T1111	3720-3725	NN	denotes	break
T1103	3726-3732	VB	denotes	invade
T1112	3732-3733	-LRB-	denotes	(
T1113	3733-3734	VBZ	denotes	s
T1114	3734-3735	-RRB-	denotes	)
T1115	3736-3739	DT	denotes	the
T1117	3740-3750	JJ	denotes	homologous
T1116	3751-3759	NN	denotes	sequence
T1118	3760-3762	IN	denotes	in
T1119	3763-3766	DT	denotes	the
T1121	3767-3773	JJ	denotes	second
T1122	3774-3780	JJ	denotes	mutant
T1123	3781-3784	NN	denotes	GFP
T1120	3785-3789	NN	denotes	copy
T1124	3789-3791	,	denotes	,
T1125	3791-3800	VBG	denotes	resulting
T1126	3801-3803	IN	denotes	in
T1127	3804-3810	NN	denotes	repair
T1128	3811-3813	IN	denotes	of
T1129	3814-3817	DT	denotes	the
T1130	3818-3821	NN	denotes	DSB
T1131	3822-3825	IN	denotes	via
T1132	3826-3827	DT	denotes	a
T1134	3828-3836	NN	denotes	homology
T1136	3836-3837	HYPH	denotes	-
T1135	3837-3845	VBN	denotes	mediated
T1137	3846-3850	NN	denotes	gene
T1138	3851-3861	NN	denotes	conversion
T1133	3862-3867	NN	denotes	event
T1139	3867-3868	.	denotes	.
T1140	3868-4005	sentence	denotes	The configuration of the GFP construct is such that homology-mediated repair often results in the formation of a functional copy of GFP.
T1141	3869-3872	DT	denotes	The
T1142	3873-3886	NN	denotes	configuration
T1144	3887-3889	IN	denotes	of
T1145	3890-3893	DT	denotes	the
T1147	3894-3897	NN	denotes	GFP
T1146	3898-3907	NN	denotes	construct
T1143	3908-3910	VBZ	denotes	is
T1148	3911-3915	JJ	denotes	such
T1149	3916-3920	IN	denotes	that
T1151	3921-3929	NN	denotes	homology
T1153	3929-3930	HYPH	denotes	-
T1152	3930-3938	VBN	denotes	mediated
T1154	3939-3945	NN	denotes	repair
T1155	3946-3951	RB	denotes	often
T1150	3952-3959	VBZ	denotes	results
T1156	3960-3962	IN	denotes	in
T1157	3963-3966	DT	denotes	the
T1158	3967-3976	NN	denotes	formation
T1159	3977-3979	IN	denotes	of
T1160	3980-3981	DT	denotes	a
T1162	3982-3992	JJ	denotes	functional
T1161	3993-3997	NN	denotes	copy
T1163	3998-4000	IN	denotes	of
T1164	4001-4004	NN	denotes	GFP
T1165	4004-4005	.	denotes	.
T1166	4005-4119	sentence	denotes	Such events can be detected by fluorescence-activated cell sorting analysis by virtue of their expression of GFP.
T1167	4006-4010	JJ	denotes	Such
T1168	4011-4017	NNS	denotes	events
T1170	4018-4021	MD	denotes	can
T1171	4022-4024	VB	denotes	be
T1169	4025-4033	VBN	denotes	detected
T1172	4034-4036	IN	denotes	by
T1173	4037-4049	NN	denotes	fluorescence
T1175	4049-4050	HYPH	denotes	-
T1174	4050-4059	VBN	denotes	activated
T1177	4060-4064	NN	denotes	cell
T1176	4065-4072	NN	denotes	sorting
T1178	4073-4081	NN	denotes	analysis
T1179	4082-4084	IN	denotes	by
T1180	4085-4091	NN	denotes	virtue
T1181	4092-4094	IN	denotes	of
T1182	4095-4100	PRP$	denotes	their
T1183	4101-4111	NN	denotes	expression
T1184	4112-4114	IN	denotes	of
T1185	4115-4118	NN	denotes	GFP
T1186	4118-4119	.	denotes	.
T1187	4119-4788	sentence	denotes	Figure 1 Recombination substrates used for assaying homology-directed repair. Cutting at the I-SceI site within the mutant GFP (SceGFP) results in a double-strand break that can be repaired through homologous gene conversion using a 3'-truncated copy of GFP as sequence donor. The mechanism results in the formation of a functional copy of the GFP gene. The model shown assumes gene conversion occurs via the synthesis-dependent annealing mechanism. Moynahan et al [24] have used such a GFP recombination substrate to demonstrate that cells with defective BRCA2 protein are deficient in their ability to repair the I-SceI-induced DSB through homologous recombination.
T1188	4571-4579	NNP	denotes	Moynahan
T1190	4580-4582	FW	denotes	et
T1191	4583-4585	FW	denotes	al
T1192	4586-4587	-LRB-	denotes	[
T1193	4587-4589	CD	denotes	24
T1194	4589-4590	-RRB-	denotes	]
T1195	4591-4595	VBP	denotes	have
T1189	4596-4600	VBN	denotes	used
T1196	4601-4605	PDT	denotes	such
T1198	4606-4607	DT	denotes	a
T1199	4608-4611	NN	denotes	GFP
T1200	4612-4625	NN	denotes	recombination
T1197	4626-4635	NN	denotes	substrate
T1201	4636-4638	TO	denotes	to
T1202	4639-4650	VB	denotes	demonstrate
T1203	4651-4655	IN	denotes	that
T1205	4656-4661	NNS	denotes	cells
T1206	4662-4666	IN	denotes	with
T1207	4667-4676	JJ	denotes	defective
T1209	4677-4682	NN	denotes	BRCA2
T1208	4683-4690	NN	denotes	protein
T1204	4691-4694	VBP	denotes	are
T1210	4695-4704	JJ	denotes	deficient
T1211	4705-4707	IN	denotes	in
T1212	4708-4713	PRP$	denotes	their
T1213	4714-4721	NN	denotes	ability
T1214	4722-4724	TO	denotes	to
T1215	4725-4731	VB	denotes	repair
T1216	4732-4735	DT	denotes	the
T1218	4736-4737	NN	denotes	I
T1220	4737-4738	HYPH	denotes	-
T1219	4738-4742	NN	denotes	SceI
T1222	4742-4743	HYPH	denotes	-
T1221	4743-4750	VBN	denotes	induced
T1217	4751-4754	NN	denotes	DSB
T1223	4755-4762	IN	denotes	through
T1224	4763-4773	JJ	denotes	homologous
T1225	4774-4787	NN	denotes	recombination
T1226	4787-4788	.	denotes	.
T1227	4788-4933	sentence	denotes	Expression of I-SceI resulted in 1 out of 1400 cells producing GFP via homologous recombination in the human pancreatic tumor cell line CAPAN-1.
T1228	4789-4799	NN	denotes	Expression
T1230	4800-4802	IN	denotes	of
T1231	4803-4804	NN	denotes	I
T1233	4804-4805	HYPH	denotes	-
T1232	4805-4809	NN	denotes	SceI
T1229	4810-4818	VBD	denotes	resulted
T1234	4819-4821	IN	denotes	in
T1235	4822-4823	CD	denotes	1
T1237	4824-4827	IN	denotes	out
T1238	4828-4830	IN	denotes	of
T1236	4831-4835	CD	denotes	1400
T1239	4836-4841	NNS	denotes	cells
T1240	4842-4851	VBG	denotes	producing
T1241	4852-4855	NN	denotes	GFP
T1242	4856-4859	IN	denotes	via
T1243	4860-4870	JJ	denotes	homologous
T1244	4871-4884	NN	denotes	recombination
T1245	4885-4887	IN	denotes	in
T1246	4888-4891	DT	denotes	the
T1248	4892-4897	JJ	denotes	human
T1250	4898-4908	JJ	denotes	pancreatic
T1251	4909-4914	NN	denotes	tumor
T1249	4915-4919	NN	denotes	cell
T1247	4920-4924	NN	denotes	line
T1252	4925-4930	NN	denotes	CAPAN
T1253	4930-4931	HYPH	denotes	-
T1254	4931-4932	CD	denotes	1
T1255	4932-4933	.	denotes	.
T1256	4933-5063	sentence	denotes	CAPAN-1 cells carry a deletion of BRCA2 on one homolog and codes for a protein truncated at amino acid 1981 on the other homolog.
T1257	4934-4939	NN	denotes	CAPAN
T1259	4939-4940	HYPH	denotes	-
T1260	4940-4941	CD	denotes	1
T1258	4942-4947	NNS	denotes	cells
T1261	4948-4953	VBP	denotes	carry
T1262	4954-4955	DT	denotes	a
T1263	4956-4964	NN	denotes	deletion
T1264	4965-4967	IN	denotes	of
T1265	4968-4973	NN	denotes	BRCA2
T1266	4974-4976	IN	denotes	on
T1267	4977-4980	CD	denotes	one
T1268	4981-4988	NN	denotes	homolog
T1269	4989-4992	CC	denotes	and
T1270	4993-4998	NNS	denotes	codes
T1271	4999-5002	IN	denotes	for
T1272	5003-5004	DT	denotes	a
T1273	5005-5012	NN	denotes	protein
T1274	5013-5022	VBN	denotes	truncated
T1275	5023-5025	IN	denotes	at
T1276	5026-5031	JJ	denotes	amino
T1277	5032-5036	NN	denotes	acid
T1278	5037-5041	CD	denotes	1981
T1279	5042-5044	IN	denotes	on
T1280	5045-5048	DT	denotes	the
T1282	5049-5054	JJ	denotes	other
T1281	5055-5062	NN	denotes	homolog
T1283	5062-5063	.	denotes	.
T1284	5063-5224	sentence	denotes	The authors indicate that the level of I-SceI-induced recombination in CAPAN-1 is over 100-fold less than that seen using other (BRCA2+) human tumor cell lines.
T1285	5064-5067	DT	denotes	The
T1286	5068-5075	NNS	denotes	authors
T1287	5076-5084	VBP	denotes	indicate
T1288	5085-5089	IN	denotes	that
T1290	5090-5093	DT	denotes	the
T1291	5094-5099	NN	denotes	level
T1292	5100-5102	IN	denotes	of
T1293	5103-5104	NN	denotes	I
T1295	5104-5105	HYPH	denotes	-
T1294	5105-5109	NN	denotes	SceI
T1297	5109-5110	HYPH	denotes	-
T1296	5110-5117	VBN	denotes	induced
T1298	5118-5131	NN	denotes	recombination
T1299	5132-5134	IN	denotes	in
T1300	5135-5140	NN	denotes	CAPAN
T1301	5140-5141	HYPH	denotes	-
T1302	5141-5142	CD	denotes	1
T1289	5143-5145	VBZ	denotes	is
T1303	5146-5150	IN	denotes	over
T1304	5151-5159	RB	denotes	100-fold
T1305	5160-5164	JJR	denotes	less
T1306	5165-5169	IN	denotes	than
T1307	5170-5174	DT	denotes	that
T1308	5175-5179	VBN	denotes	seen
T1309	5180-5185	VBG	denotes	using
T1310	5186-5191	JJ	denotes	other
T1312	5192-5193	-LRB-	denotes	(
T1311	5193-5199	JJ	denotes	BRCA2+
T1314	5199-5200	-RRB-	denotes	)
T1315	5201-5206	JJ	denotes	human
T1316	5207-5212	NN	denotes	tumor
T1317	5213-5217	NN	denotes	cell
T1313	5218-5223	NNS	denotes	lines
T1318	5223-5224	.	denotes	.
T1319	5224-5401	sentence	denotes	The different lines examined, however, are very likely to differ genetically from CAPAN-1 cells not only at the BRCA2 locus, but also at a very large number of additional loci.
T1320	5225-5228	DT	denotes	The
T1322	5229-5238	JJ	denotes	different
T1321	5239-5244	NNS	denotes	lines
T1324	5245-5253	VBN	denotes	examined
T1325	5253-5255	,	denotes	,
T1326	5255-5262	RB	denotes	however
T1327	5262-5264	,	denotes	,
T1323	5264-5267	VBP	denotes	are
T1328	5268-5272	RB	denotes	very
T1329	5273-5279	JJ	denotes	likely
T1330	5280-5282	TO	denotes	to
T1331	5283-5289	VB	denotes	differ
T1332	5290-5301	RB	denotes	genetically
T1333	5302-5306	IN	denotes	from
T1334	5307-5312	NN	denotes	CAPAN
T1336	5312-5313	HYPH	denotes	-
T1337	5313-5314	CD	denotes	1
T1335	5315-5320	NNS	denotes	cells
T1338	5321-5324	RB	denotes	not
T1340	5325-5329	RB	denotes	only
T1339	5330-5332	IN	denotes	at
T1341	5333-5336	DT	denotes	the
T1343	5337-5342	NN	denotes	BRCA2
T1342	5343-5348	NN	denotes	locus
T1344	5348-5350	,	denotes	,
T1345	5350-5353	CC	denotes	but
T1346	5354-5358	RB	denotes	also
T1347	5359-5361	IN	denotes	at
T1348	5362-5363	DT	denotes	a
T1350	5364-5368	RB	denotes	very
T1351	5369-5374	JJ	denotes	large
T1349	5375-5381	NN	denotes	number
T1352	5382-5384	IN	denotes	of
T1353	5385-5395	JJ	denotes	additional
T1354	5396-5400	NNS	denotes	loci
T1355	5400-5401	.	denotes	.
T1356	5401-5549	sentence	denotes	This raises the possibility that some or even all of the recombinational repair defect seen in CAPAN-1 could be due to mutations at non-BRCA2 loci.
T1357	5402-5406	DT	denotes	This
T1358	5407-5413	VBZ	denotes	raises
T1359	5414-5417	DT	denotes	the
T1360	5418-5429	NN	denotes	possibility
T1361	5430-5434	IN	denotes	that
T1363	5435-5439	DT	denotes	some
T1364	5440-5442	CC	denotes	or
T1365	5443-5447	RB	denotes	even
T1366	5448-5451	DT	denotes	all
T1367	5452-5454	IN	denotes	of
T1368	5455-5458	DT	denotes	the
T1370	5459-5474	JJ	denotes	recombinational
T1371	5475-5481	NN	denotes	repair
T1369	5482-5488	NN	denotes	defect
T1372	5489-5493	VBN	denotes	seen
T1373	5494-5496	IN	denotes	in
T1374	5497-5502	NN	denotes	CAPAN
T1375	5502-5503	HYPH	denotes	-
T1376	5503-5504	CD	denotes	1
T1377	5505-5510	MD	denotes	could
T1362	5511-5513	VB	denotes	be
T1378	5514-5517	IN	denotes	due
T1379	5518-5520	IN	denotes	to
T1380	5521-5530	NNS	denotes	mutations
T1381	5531-5533	IN	denotes	at
T1382	5534-5543	JJ	denotes	non-BRCA2
T1383	5544-5548	NNS	denotes	loci
T1384	5548-5549	.	denotes	.
T1385	5549-5770	sentence	denotes	While Moynahan et al were careful to point out this problem, two sets of results argue against the possibility that the recombinational repair deficiency of CAPAN-1 cells is completely independent of its defect in BRCA2.
T1386	5550-5555	IN	denotes	While
T1388	5556-5564	NNP	denotes	Moynahan
T1389	5565-5567	FW	denotes	et
T1390	5568-5570	FW	denotes	al
T1387	5571-5575	VBD	denotes	were
T1392	5576-5583	JJ	denotes	careful
T1393	5584-5586	TO	denotes	to
T1394	5587-5592	VB	denotes	point
T1395	5593-5596	RP	denotes	out
T1396	5597-5601	DT	denotes	this
T1397	5602-5609	NN	denotes	problem
T1398	5609-5611	,	denotes	,
T1399	5611-5614	CD	denotes	two
T1400	5615-5619	NNS	denotes	sets
T1401	5620-5622	IN	denotes	of
T1402	5623-5630	NNS	denotes	results
T1391	5631-5636	VBP	denotes	argue
T1403	5637-5644	IN	denotes	against
T1404	5645-5648	DT	denotes	the
T1405	5649-5660	NN	denotes	possibility
T1406	5661-5665	IN	denotes	that
T1408	5666-5669	DT	denotes	the
T1410	5670-5685	JJ	denotes	recombinational
T1411	5686-5692	NN	denotes	repair
T1409	5693-5703	NN	denotes	deficiency
T1412	5704-5706	IN	denotes	of
T1413	5707-5712	NN	denotes	CAPAN
T1415	5712-5713	HYPH	denotes	-
T1416	5713-5714	CD	denotes	1
T1414	5715-5720	NNS	denotes	cells
T1407	5721-5723	VBZ	denotes	is
T1417	5724-5734	RB	denotes	completely
T1418	5735-5746	JJ	denotes	independent
T1419	5747-5749	IN	denotes	of
T1420	5750-5753	PRP$	denotes	its
T1421	5754-5760	NN	denotes	defect
T1422	5761-5763	IN	denotes	in
T1423	5764-5769	NN	denotes	BRCA2
T1424	5769-5770	.	denotes	.
T1425	5770-5897	sentence	denotes	First, in the same study [24], recombination in a mouse ES cell line that expresses only truncated BRCA2 protein was measured.
T1426	5771-5776	RB	denotes	First
T1428	5776-5778	,	denotes	,
T1429	5778-5780	IN	denotes	in
T1430	5781-5784	DT	denotes	the
T1432	5785-5789	JJ	denotes	same
T1431	5790-5795	NN	denotes	study
T1433	5796-5797	-LRB-	denotes	[
T1434	5797-5799	CD	denotes	24
T1435	5799-5800	-RRB-	denotes	]
T1436	5800-5802	,	denotes	,
T1437	5802-5815	NN	denotes	recombination
T1438	5816-5818	IN	denotes	in
T1439	5819-5820	DT	denotes	a
T1441	5821-5826	NN	denotes	mouse
T1442	5827-5829	NN	denotes	ES
T1443	5830-5834	NN	denotes	cell
T1440	5835-5839	NN	denotes	line
T1444	5840-5844	WDT	denotes	that
T1445	5845-5854	VBZ	denotes	expresses
T1446	5855-5859	RB	denotes	only
T1448	5860-5869	VBN	denotes	truncated
T1449	5870-5875	NN	denotes	BRCA2
T1447	5876-5883	NN	denotes	protein
T1450	5884-5887	VBD	denotes	was
T1427	5888-5896	VBN	denotes	measured
T1451	5896-5897	.	denotes	.
T1452	5897-6058	sentence	denotes	This line was found to have lower recombination efficiency than isogenic cells expressing full-length BRCA2 (the defect observed was about fivefold to sixfold).
T1453	5898-5902	DT	denotes	This
T1454	5903-5907	NN	denotes	line
T1456	5908-5911	VBD	denotes	was
T1455	5912-5917	VBN	denotes	found
T1457	5918-5920	TO	denotes	to
T1458	5921-5925	VB	denotes	have
T1459	5926-5931	JJR	denotes	lower
T1461	5932-5945	NN	denotes	recombination
T1460	5946-5956	NN	denotes	efficiency
T1462	5957-5961	IN	denotes	than
T1463	5962-5970	JJ	denotes	isogenic
T1464	5971-5976	NNS	denotes	cells
T1465	5977-5987	VBG	denotes	expressing
T1466	5988-5992	JJ	denotes	full
T1468	5992-5993	HYPH	denotes	-
T1467	5993-5999	NN	denotes	length
T1469	6000-6005	NN	denotes	BRCA2
T1470	6006-6007	-LRB-	denotes	(
T1472	6007-6010	DT	denotes	the
T1473	6011-6017	NN	denotes	defect
T1474	6018-6026	VBN	denotes	observed
T1471	6027-6030	VBD	denotes	was
T1475	6031-6036	RB	denotes	about
T1477	6037-6045	RB	denotes	fivefold
T1478	6046-6048	IN	denotes	to
T1476	6049-6056	RB	denotes	sixfold
T1479	6056-6057	-RRB-	denotes	)
T1480	6057-6058	.	denotes	.
T1481	6058-6257	sentence	denotes	Second, in an independent study, Powell was able to compare derivatives of CAPAN-1 that differed only in their ability to express full-length BRCA2 protein (S. Powell, personal communication, 2001).
T1482	6059-6065	RB	denotes	Second
T1484	6065-6067	,	denotes	,
T1485	6067-6069	IN	denotes	in
T1486	6070-6072	DT	denotes	an
T1488	6073-6084	JJ	denotes	independent
T1487	6085-6090	NN	denotes	study
T1489	6090-6092	,	denotes	,
T1490	6092-6098	NNP	denotes	Powell
T1483	6099-6102	VBD	denotes	was
T1491	6103-6107	JJ	denotes	able
T1492	6108-6110	TO	denotes	to
T1493	6111-6118	VB	denotes	compare
T1494	6119-6130	NNS	denotes	derivatives
T1495	6131-6133	IN	denotes	of
T1496	6134-6139	NN	denotes	CAPAN
T1497	6139-6140	HYPH	denotes	-
T1498	6140-6141	CD	denotes	1
T1499	6142-6146	WDT	denotes	that
T1500	6147-6155	VBD	denotes	differed
T1501	6156-6160	RB	denotes	only
T1502	6161-6163	IN	denotes	in
T1503	6164-6169	PRP$	denotes	their
T1504	6170-6177	NN	denotes	ability
T1505	6178-6180	TO	denotes	to
T1506	6181-6188	VB	denotes	express
T1507	6189-6193	JJ	denotes	full
T1509	6193-6194	HYPH	denotes	-
T1508	6194-6200	NN	denotes	length
T1511	6201-6206	NN	denotes	BRCA2
T1510	6207-6214	NN	denotes	protein
T1512	6215-6216	-LRB-	denotes	(
T1514	6216-6218	NNP	denotes	S.
T1513	6219-6225	NNP	denotes	Powell
T1515	6225-6227	,	denotes	,
T1516	6227-6235	JJ	denotes	personal
T1517	6236-6249	NN	denotes	communication
T1518	6249-6251	,	denotes	,
T1519	6251-6255	CD	denotes	2001
T1520	6255-6256	-RRB-	denotes	)
T1521	6256-6257	.	denotes	.
T1522	6257-6446	sentence	denotes	In these experiments, the derivative expressing full-length BRCA2 yielded 10-fold more recombinants than controls expressing only truncated BRCA2 (S. Powell, personal communication, 2001).
T1523	6258-6260	IN	denotes	In
T1525	6261-6266	DT	denotes	these
T1526	6267-6278	NNS	denotes	experiments
T1527	6278-6280	,	denotes	,
T1528	6280-6283	DT	denotes	the
T1529	6284-6294	NN	denotes	derivative
T1530	6295-6305	VBG	denotes	expressing
T1531	6306-6310	JJ	denotes	full
T1533	6310-6311	HYPH	denotes	-
T1532	6311-6317	NN	denotes	length
T1534	6318-6323	NN	denotes	BRCA2
T1524	6324-6331	VBD	denotes	yielded
T1535	6332-6339	RB	denotes	10-fold
T1536	6340-6344	JJR	denotes	more
T1537	6345-6357	NNS	denotes	recombinants
T1538	6358-6362	IN	denotes	than
T1539	6363-6371	NNS	denotes	controls
T1540	6372-6382	VBG	denotes	expressing
T1541	6383-6387	RB	denotes	only
T1543	6388-6397	VBN	denotes	truncated
T1542	6398-6403	NN	denotes	BRCA2
T1544	6404-6405	-LRB-	denotes	(
T1546	6405-6407	NNP	denotes	S.
T1545	6408-6414	NNP	denotes	Powell
T1547	6414-6416	,	denotes	,
T1548	6416-6424	JJ	denotes	personal
T1549	6425-6438	NN	denotes	communication
T1550	6438-6440	,	denotes	,
T1551	6440-6444	CD	denotes	2001
T1552	6444-6445	-RRB-	denotes	)
T1553	6445-6446	.	denotes	.
T1554	6446-6742	sentence	denotes	While the experimental design of these experiments was somewhat different from that of Moyna-han et al [24], the results raise the possibility that CAPAN-1 cells have more than one mutation that lowers the efficiency of recombinational repair relative to that observed in other human cell lines.
T1555	6447-6452	IN	denotes	While
T1557	6453-6456	DT	denotes	the
T1559	6457-6469	JJ	denotes	experimental
T1558	6470-6476	NN	denotes	design
T1560	6477-6479	IN	denotes	of
T1561	6480-6485	DT	denotes	these
T1562	6486-6497	NNS	denotes	experiments
T1556	6498-6501	VBD	denotes	was
T1564	6502-6510	RB	denotes	somewhat
T1565	6511-6520	JJ	denotes	different
T1566	6521-6525	IN	denotes	from
T1567	6526-6530	DT	denotes	that
T1568	6531-6533	IN	denotes	of
T1569	6534-6539	NNP	denotes	Moyna
T1571	6539-6540	HYPH	denotes	-
T1570	6540-6543	NNP	denotes	han
T1572	6544-6546	FW	denotes	et
T1573	6547-6549	FW	denotes	al
T1574	6550-6551	-LRB-	denotes	[
T1575	6551-6553	CD	denotes	24
T1576	6553-6554	-RRB-	denotes	]
T1577	6554-6556	,	denotes	,
T1578	6556-6559	DT	denotes	the
T1579	6560-6567	NNS	denotes	results
T1563	6568-6573	VBP	denotes	raise
T1580	6574-6577	DT	denotes	the
T1581	6578-6589	NN	denotes	possibility
T1582	6590-6594	IN	denotes	that
T1584	6595-6600	NN	denotes	CAPAN
T1586	6600-6601	HYPH	denotes	-
T1587	6601-6602	CD	denotes	1
T1585	6603-6608	NNS	denotes	cells
T1583	6609-6613	VBP	denotes	have
T1588	6614-6618	JJR	denotes	more
T1590	6619-6623	IN	denotes	than
T1589	6624-6627	CD	denotes	one
T1591	6628-6636	NN	denotes	mutation
T1592	6637-6641	WDT	denotes	that
T1593	6642-6648	VBZ	denotes	lowers
T1594	6649-6652	DT	denotes	the
T1595	6653-6663	NN	denotes	efficiency
T1596	6664-6666	IN	denotes	of
T1597	6667-6682	JJ	denotes	recombinational
T1598	6683-6689	NN	denotes	repair
T1599	6690-6698	JJ	denotes	relative
T1600	6699-6701	IN	denotes	to
T1601	6702-6706	DT	denotes	that
T1602	6707-6715	VBN	denotes	observed
T1603	6716-6718	IN	denotes	in
T1604	6719-6724	JJ	denotes	other
T1606	6725-6730	JJ	denotes	human
T1607	6731-6735	NN	denotes	cell
T1605	6736-6741	NNS	denotes	lines
T1608	6741-6742	.	denotes	.
T1609	6742-6923	sentence	denotes	Conversely, the BRCA2 defect in CAPAN-1 could be fully responsible for the 100-fold defect in recombination if the level of BRCA2 complementation observed by Powell was incomplete.
T1610	6743-6753	RB	denotes	Conversely
T1612	6753-6755	,	denotes	,
T1613	6755-6758	DT	denotes	the
T1615	6759-6764	NN	denotes	BRCA2
T1614	6765-6771	NN	denotes	defect
T1616	6772-6774	IN	denotes	in
T1617	6775-6780	NN	denotes	CAPAN
T1618	6780-6781	HYPH	denotes	-
T1619	6781-6782	CD	denotes	1
T1620	6783-6788	MD	denotes	could
T1611	6789-6791	VB	denotes	be
T1621	6792-6797	RB	denotes	fully
T1622	6798-6809	JJ	denotes	responsible
T1623	6810-6813	IN	denotes	for
T1624	6814-6817	DT	denotes	the
T1626	6818-6826	JJ	denotes	100-fold
T1625	6827-6833	NN	denotes	defect
T1627	6834-6836	IN	denotes	in
T1628	6837-6850	NN	denotes	recombination
T1629	6851-6853	IN	denotes	if
T1631	6854-6857	DT	denotes	the
T1632	6858-6863	NN	denotes	level
T1633	6864-6866	IN	denotes	of
T1634	6867-6872	NN	denotes	BRCA2
T1635	6873-6888	NN	denotes	complementation
T1636	6889-6897	VBN	denotes	observed
T1637	6898-6900	IN	denotes	by
T1638	6901-6907	NNP	denotes	Powell
T1630	6908-6911	VBD	denotes	was
T1639	6912-6922	JJ	denotes	incomplete
T1640	6922-6923	.	denotes	.
T1641	6923-7216	sentence	denotes	Furthermore, the discrepancy between the 100-fold difference between CAPAN-1 and the other human lines as compared with the fivefold to sixfold difference between the Brca2lex1/lex2cells and Brca2+/+ ES cells might simply be accounted for by the fact that Brca2lex1/lex2 is not a null allele.
T1642	6924-6935	RB	denotes	Furthermore
T1644	6935-6937	,	denotes	,
T1645	6937-6940	DT	denotes	the
T1646	6941-6952	NN	denotes	discrepancy
T1647	6953-6960	IN	denotes	between
T1648	6961-6964	DT	denotes	the
T1650	6965-6973	JJ	denotes	100-fold
T1649	6974-6984	NN	denotes	difference
T1651	6985-6992	IN	denotes	between
T1652	6993-6998	NN	denotes	CAPAN
T1653	6998-6999	HYPH	denotes	-
T1654	6999-7000	CD	denotes	1
T1655	7001-7004	CC	denotes	and
T1656	7005-7008	DT	denotes	the
T1658	7009-7014	JJ	denotes	other
T1659	7015-7020	JJ	denotes	human
T1657	7021-7026	NNS	denotes	lines
T1660	7027-7029	IN	denotes	as
T1661	7030-7038	VBN	denotes	compared
T1662	7039-7043	IN	denotes	with
T1663	7044-7047	DT	denotes	the
T1665	7048-7056	JJ	denotes	fivefold
T1667	7057-7059	IN	denotes	to
T1666	7060-7067	JJ	denotes	sixfold
T1664	7068-7078	NN	denotes	difference
T1668	7079-7086	IN	denotes	between
T1669	7087-7090	DT	denotes	the
T1671	7091-7100	NN	denotes	Brca2lex1
T1673	7100-7101	HYPH	denotes	/
T1672	7101-7105	NN	denotes	lex2
T1670	7105-7110	NNS	denotes	cells
T1674	7111-7114	CC	denotes	and
T1675	7115-7120	NN	denotes	Brca2
T1677	7120-7121	SYM	denotes	+
T1678	7121-7122	HYPH	denotes	/
T1679	7122-7123	SYM	denotes	+
T1680	7124-7126	NN	denotes	ES
T1676	7127-7132	NNS	denotes	cells
T1681	7133-7138	MD	denotes	might
T1682	7139-7145	RB	denotes	simply
T1683	7146-7148	VB	denotes	be
T1643	7149-7158	VBN	denotes	accounted
T1684	7159-7162	IN	denotes	for
T1685	7163-7165	IN	denotes	by
T1686	7166-7169	DT	denotes	the
T1687	7170-7174	NN	denotes	fact
T1688	7175-7179	IN	denotes	that
T1690	7180-7189	NN	denotes	Brca2lex1
T1692	7189-7190	HYPH	denotes	/
T1691	7190-7194	NN	denotes	lex2
T1689	7195-7197	VBZ	denotes	is
T1693	7198-7201	RB	denotes	not
T1694	7202-7203	DT	denotes	a
T1696	7204-7208	JJ	denotes	null
T1695	7209-7215	NN	denotes	allele
T1697	7215-7216	.	denotes	.
T1698	7216-7323	sentence	denotes	Additional studies are likely to shed light on the efficiency of BRCA2-independent recombination pathways.
T1699	7217-7227	JJ	denotes	Additional
T1700	7228-7235	NNS	denotes	studies
T1701	7236-7239	VBP	denotes	are
T1702	7240-7246	JJ	denotes	likely
T1703	7247-7249	TO	denotes	to
T1704	7250-7254	VB	denotes	shed
T1705	7255-7260	NN	denotes	light
T1706	7261-7263	IN	denotes	on
T1707	7264-7267	DT	denotes	the
T1708	7268-7278	NN	denotes	efficiency
T1709	7279-7281	IN	denotes	of
T1710	7282-7287	NN	denotes	BRCA2
T1712	7287-7288	HYPH	denotes	-
T1711	7288-7299	JJ	denotes	independent
T1714	7300-7313	NN	denotes	recombination
T1713	7314-7322	NNS	denotes	pathways
T1715	7322-7323	.	denotes	.
T1716	7323-7457	sentence	denotes	Taken together, the results indicate that BRCA2 is indeed required for high levels of recombinational repair in both human and mouse.
T1717	7324-7329	VBN	denotes	Taken
T1719	7330-7338	RB	denotes	together
T1720	7338-7340	,	denotes	,
T1721	7340-7343	DT	denotes	the
T1722	7344-7351	NNS	denotes	results
T1718	7352-7360	VBP	denotes	indicate
T1723	7361-7365	IN	denotes	that
T1725	7366-7371	NN	denotes	BRCA2
T1726	7372-7374	VBZ	denotes	is
T1727	7375-7381	RB	denotes	indeed
T1724	7382-7390	VBN	denotes	required
T1728	7391-7394	IN	denotes	for
T1729	7395-7399	JJ	denotes	high
T1730	7400-7406	NNS	denotes	levels
T1731	7407-7409	IN	denotes	of
T1732	7410-7425	JJ	denotes	recombinational
T1733	7426-7432	NN	denotes	repair
T1734	7433-7435	IN	denotes	in
T1735	7436-7440	CC	denotes	both
T1737	7441-7446	JJ	denotes	human
T1738	7447-7450	CC	denotes	and
T1736	7451-7456	NN	denotes	mouse
T1739	7456-7457	.	denotes	.
T2154	7499-7502	DT	denotes	The
T2156	7503-7509	JJ	denotes	second
T2155	7510-7515	NN	denotes	paper
T2158	7516-7525	VBG	denotes	providing
T2159	7526-7534	NN	denotes	evidence
T2160	7534-7536	,	denotes	,
T2161	7536-7538	IN	denotes	by
T2162	7539-7545	NNP	denotes	Davies
T2163	7546-7548	FW	denotes	et
T2164	7549-7551	FW	denotes	al
T2165	7552-7553	-LRB-	denotes	[
T2166	7553-7555	CD	denotes	26
T2167	7555-7556	-RRB-	denotes	]
T2168	7556-7558	,	denotes	,
T2157	7558-7560	VBZ	denotes	is
T2169	7561-7562	DT	denotes	a
T2171	7563-7574	JJ	denotes	biochemical
T2170	7575-7580	NN	denotes	study
T2172	7581-7583	IN	denotes	of
T2173	7584-7587	DT	denotes	the
T2174	7588-7599	NN	denotes	interaction
T2175	7600-7607	IN	denotes	between
T2176	7608-7611	DT	denotes	the
T2178	7612-7622	JJ	denotes	homologous
T2179	7623-7634	NN	denotes	recombinase
T2177	7635-7640	NN	denotes	RAD51
T2180	7641-7644	CC	denotes	and
T2181	7645-7646	DT	denotes	a
T2182	7647-7654	NN	denotes	peptide
T2183	7655-7665	VBG	denotes	consisting
T2184	7666-7668	IN	denotes	of
T2185	7669-7672	CD	denotes	one
T2186	7673-7675	IN	denotes	of
T2187	7676-7679	DT	denotes	the
T2189	7680-7685	CD	denotes	eight
T2190	7686-7689	NN	denotes	BRC
T2188	7690-7697	NNS	denotes	repeats
T2191	7698-7702	IN	denotes	from
T2192	7703-7708	JJ	denotes	human
T2193	7709-7714	NN	denotes	BRCA2
T2194	7714-7715	.	denotes	.
T2195	7715-7842	sentence	denotes	Expression of a single BRC repeat (BRC4) had previously been reported to act as an inhibitor of DNA repair by Chen et al [27].
T2196	7716-7726	NN	denotes	Expression
T2198	7727-7729	IN	denotes	of
T2199	7730-7731	DT	denotes	a
T2201	7732-7738	JJ	denotes	single
T2202	7739-7742	NN	denotes	BRC
T2200	7743-7749	NN	denotes	repeat
T2203	7750-7751	-LRB-	denotes	(
T2204	7751-7755	NN	denotes	BRC4
T2205	7755-7756	-RRB-	denotes	)
T2206	7757-7760	VBD	denotes	had
T2207	7761-7771	RB	denotes	previously
T2208	7772-7776	VBN	denotes	been
T2197	7777-7785	VBN	denotes	reported
T2209	7786-7788	TO	denotes	to
T2210	7789-7792	VB	denotes	act
T2211	7793-7795	IN	denotes	as
T2212	7796-7798	DT	denotes	an
T2213	7799-7808	NN	denotes	inhibitor
T2214	7809-7811	IN	denotes	of
T2215	7812-7815	NN	denotes	DNA
T2216	7816-7822	NN	denotes	repair
T2217	7823-7825	IN	denotes	by
T2218	7826-7830	NNP	denotes	Chen
T2219	7831-7833	FW	denotes	et
T2220	7834-7836	FW	denotes	al
T2221	7837-7838	-LRB-	denotes	[
T2222	7838-7840	CD	denotes	27
T2223	7840-7841	-RRB-	denotes	]
T2224	7841-7842	.	denotes	.
T2225	7842-8110	sentence	denotes	These investigators showed that expression of constructs containing the BRC4 repeat in MCF-7 cells enhances the radiosensitivity of cells and blocks both the G2/M delay associated with damage and the ability of the transfected cells to assembly subnuclear RAD51 foci.
T2226	7843-7848	DT	denotes	These
T2227	7849-7862	NNS	denotes	investigators
T2228	7863-7869	VBD	denotes	showed
T2229	7870-7874	IN	denotes	that
T2231	7875-7885	NN	denotes	expression
T2232	7886-7888	IN	denotes	of
T2233	7889-7899	NNS	denotes	constructs
T2234	7900-7910	VBG	denotes	containing
T2235	7911-7914	DT	denotes	the
T2237	7915-7919	NN	denotes	BRC4
T2236	7920-7926	NN	denotes	repeat
T2238	7927-7929	IN	denotes	in
T2239	7930-7933	NN	denotes	MCF
T2241	7933-7934	HYPH	denotes	-
T2242	7934-7935	CD	denotes	7
T2240	7936-7941	NNS	denotes	cells
T2230	7942-7950	VBZ	denotes	enhances
T2243	7951-7954	DT	denotes	the
T2244	7955-7971	NN	denotes	radiosensitivity
T2245	7972-7974	IN	denotes	of
T2246	7975-7980	NNS	denotes	cells
T2247	7981-7984	CC	denotes	and
T2248	7985-7991	VBZ	denotes	blocks
T2249	7992-7996	CC	denotes	both
T2251	7997-8000	DT	denotes	the
T2252	8001-8003	NN	denotes	G2
T2254	8003-8004	HYPH	denotes	/
T2253	8004-8005	NN	denotes	M
T2250	8006-8011	NN	denotes	delay
T2255	8012-8022	VBN	denotes	associated
T2256	8023-8027	IN	denotes	with
T2257	8028-8034	NN	denotes	damage
T2258	8035-8038	CC	denotes	and
T2259	8039-8042	DT	denotes	the
T2260	8043-8050	NN	denotes	ability
T2261	8051-8053	IN	denotes	of
T2262	8054-8057	DT	denotes	the
T2264	8058-8069	VBN	denotes	transfected
T2263	8070-8075	NNS	denotes	cells
T2265	8076-8078	TO	denotes	to
T2266	8079-8087	VB	denotes	assembly
T2267	8088-8098	JJ	denotes	subnuclear
T2269	8099-8104	NN	denotes	RAD51
T2268	8105-8109	NNS	denotes	foci
T2270	8109-8110	.	denotes	.
T2271	8110-8271	sentence	denotes	In the paper by Davies et al, data provide evidence for a plausible mechanism of the dominant negative effect associated with expression of a single BRC repeat.
T2272	8111-8113	IN	denotes	In
T2274	8114-8117	DT	denotes	the
T2275	8118-8123	NN	denotes	paper
T2276	8124-8126	IN	denotes	by
T2277	8127-8133	NNP	denotes	Davies
T2278	8134-8136	FW	denotes	et
T2279	8137-8139	FW	denotes	al
T2280	8139-8141	,	denotes	,
T2281	8141-8145	NNS	denotes	data
T2273	8146-8153	VBP	denotes	provide
T2282	8154-8162	NN	denotes	evidence
T2283	8163-8166	IN	denotes	for
T2284	8167-8168	DT	denotes	a
T2286	8169-8178	JJ	denotes	plausible
T2285	8179-8188	NN	denotes	mechanism
T2287	8189-8191	IN	denotes	of
T2288	8192-8195	DT	denotes	the
T2290	8196-8204	JJ	denotes	dominant
T2291	8205-8213	JJ	denotes	negative
T2289	8214-8220	NN	denotes	effect
T2292	8221-8231	VBN	denotes	associated
T2293	8232-8236	IN	denotes	with
T2294	8237-8247	NN	denotes	expression
T2295	8248-8250	IN	denotes	of
T2296	8251-8252	DT	denotes	a
T2298	8253-8259	JJ	denotes	single
T2299	8260-8263	NN	denotes	BRC
T2297	8264-8270	NN	denotes	repeat
T2300	8270-8271	.	denotes	.
T2301	8271-8443	sentence	denotes	DNA binding assays and electron microscopy methods were used to show that a BRC3 peptide interferes with the ability of RAD51 to assemble into oligomeric filaments on DNA.
T2302	8272-8275	NN	denotes	DNA
T2304	8276-8283	NN	denotes	binding
T2303	8284-8290	NNS	denotes	assays
T2306	8291-8294	CC	denotes	and
T2307	8295-8303	NN	denotes	electron
T2308	8304-8314	NN	denotes	microscopy
T2309	8315-8322	NNS	denotes	methods
T2310	8323-8327	VBD	denotes	were
T2305	8328-8332	VBN	denotes	used
T2311	8333-8335	TO	denotes	to
T2312	8336-8340	VB	denotes	show
T2313	8341-8345	IN	denotes	that
T2315	8346-8347	DT	denotes	a
T2317	8348-8352	NN	denotes	BRC3
T2316	8353-8360	NN	denotes	peptide
T2314	8361-8371	VBZ	denotes	interferes
T2318	8372-8376	IN	denotes	with
T2319	8377-8380	DT	denotes	the
T2320	8381-8388	NN	denotes	ability
T2321	8389-8391	IN	denotes	of
T2322	8392-8397	NN	denotes	RAD51
T2323	8398-8400	TO	denotes	to
T2324	8401-8409	VB	denotes	assemble
T2325	8410-8414	IN	denotes	into
T2326	8415-8425	JJ	denotes	oligomeric
T2327	8426-8435	NNS	denotes	filaments
T2328	8436-8438	IN	denotes	on
T2329	8439-8442	NN	denotes	DNA
T2330	8442-8443	.	denotes	.
T2331	8443-8514	sentence	denotes	A BRC4 peptide was also reported to inhibit the RAD51–DNA interaction.
T2332	8444-8445	DT	denotes	A
T2334	8446-8450	NN	denotes	BRC4
T2333	8451-8458	NN	denotes	peptide
T2336	8459-8462	VBD	denotes	was
T2337	8463-8467	RB	denotes	also
T2335	8468-8476	VBN	denotes	reported
T2338	8477-8479	TO	denotes	to
T2339	8480-8487	VB	denotes	inhibit
T2340	8488-8491	DT	denotes	the
T2342	8492-8497	NN	denotes	RAD51
T2344	8497-8498	HYPH	denotes	–
T2343	8498-8501	NN	denotes	DNA
T2341	8502-8513	NN	denotes	interaction
T2345	8513-8514	.	denotes	.
T2346	8514-8663	sentence	denotes	These experiments raise the possibility that the full-length BRCA2 protein could act as a negative regulator of inappropriate RAD51–DNA interaction.
T2347	8515-8520	DT	denotes	These
T2348	8521-8532	NNS	denotes	experiments
T2349	8533-8538	VBP	denotes	raise
T2350	8539-8542	DT	denotes	the
T2351	8543-8554	NN	denotes	possibility
T2352	8555-8559	IN	denotes	that
T2354	8560-8563	DT	denotes	the
T2356	8564-8568	JJ	denotes	full
T2358	8568-8569	HYPH	denotes	-
T2357	8569-8575	NN	denotes	length
T2359	8576-8581	NN	denotes	BRCA2
T2355	8582-8589	NN	denotes	protein
T2360	8590-8595	MD	denotes	could
T2353	8596-8599	VB	denotes	act
T2361	8600-8602	IN	denotes	as
T2362	8603-8604	DT	denotes	a
T2364	8605-8613	JJ	denotes	negative
T2363	8614-8623	NN	denotes	regulator
T2365	8624-8626	IN	denotes	of
T2366	8627-8640	JJ	denotes	inappropriate
T2368	8641-8646	NN	denotes	RAD51
T2370	8646-8647	HYPH	denotes	–
T2369	8647-8650	NN	denotes	DNA
T2367	8651-8662	NN	denotes	interaction
T2371	8662-8663	.	denotes	.
T2372	8663-8847	sentence	denotes	The final experiment reported by Davies et al [26] is particularly important with respect to understanding the mechanism(s) through which BRCA2 promotes RAD51-dependent recombination.
T2373	8664-8667	DT	denotes	The
T2375	8668-8673	JJ	denotes	final
T2374	8674-8684	NN	denotes	experiment
T2377	8685-8693	VBN	denotes	reported
T2378	8694-8696	IN	denotes	by
T2379	8697-8703	NNP	denotes	Davies
T2380	8704-8706	FW	denotes	et
T2381	8707-8709	FW	denotes	al
T2382	8710-8711	-LRB-	denotes	[
T2383	8711-8713	CD	denotes	26
T2384	8713-8714	-RRB-	denotes	]
T2376	8715-8717	VBZ	denotes	is
T2385	8718-8730	RB	denotes	particularly
T2386	8731-8740	JJ	denotes	important
T2387	8741-8745	IN	denotes	with
T2388	8746-8753	NN	denotes	respect
T2389	8754-8756	IN	denotes	to
T2390	8757-8770	VBG	denotes	understanding
T2391	8771-8774	DT	denotes	the
T2393	8775-8784	NN	denotes	mechanism
T2394	8784-8785	-LRB-	denotes	(
T2392	8785-8786	NNS	denotes	s
T2395	8786-8787	-RRB-	denotes	)
T2396	8788-8795	IN	denotes	through
T2398	8796-8801	WDT	denotes	which
T2399	8802-8807	NN	denotes	BRCA2
T2397	8808-8816	VBZ	denotes	promotes
T2400	8817-8822	NN	denotes	RAD51
T2402	8822-8823	HYPH	denotes	-
T2401	8823-8832	JJ	denotes	dependent
T2403	8833-8846	NN	denotes	recombination
T2404	8846-8847	.	denotes	.
T2405	8847-8963	sentence	denotes	The localization of RAD51 was examined in CAPAN-1 cells, the same human tumor line examined by Moynahan et al [24].
T2406	8848-8851	DT	denotes	The
T2407	8852-8864	NN	denotes	localization
T2409	8865-8867	IN	denotes	of
T2410	8868-8873	NN	denotes	RAD51
T2411	8874-8877	VBD	denotes	was
T2408	8878-8886	VBN	denotes	examined
T2412	8887-8889	IN	denotes	in
T2413	8890-8895	NN	denotes	CAPAN
T2415	8895-8896	HYPH	denotes	-
T2416	8896-8897	CD	denotes	1
T2414	8898-8903	NNS	denotes	cells
T2417	8903-8905	,	denotes	,
T2418	8905-8908	DT	denotes	the
T2420	8909-8913	JJ	denotes	same
T2421	8914-8919	JJ	denotes	human
T2422	8920-8925	NN	denotes	tumor
T2419	8926-8930	NN	denotes	line
T2423	8931-8939	VBN	denotes	examined
T2424	8940-8942	IN	denotes	by
T2425	8943-8951	NNP	denotes	Moynahan
T2426	8952-8954	FW	denotes	et
T2427	8955-8957	FW	denotes	al
T2428	8958-8959	-LRB-	denotes	[
T2429	8959-8961	CD	denotes	24
T2430	8961-8962	-RRB-	denotes	]
T2431	8962-8963	.	denotes	.
T2432	8963-9198	sentence	denotes	It had been shown previously that the small amount of truncated BRCA2 protein expressed by CAPAN-1 cells was mislocalized to the cytoplasm, consistent with the fact that the truncation protein lacks a nuclear localization signal [28].
T2433	8964-8966	PRP	denotes	It
T2435	8967-8970	VBD	denotes	had
T2436	8971-8975	VBN	denotes	been
T2434	8976-8981	VBN	denotes	shown
T2437	8982-8992	RB	denotes	previously
T2438	8993-8997	IN	denotes	that
T2440	8998-9001	DT	denotes	the
T2442	9002-9007	JJ	denotes	small
T2441	9008-9014	NN	denotes	amount
T2443	9015-9017	IN	denotes	of
T2444	9018-9027	VBN	denotes	truncated
T2446	9028-9033	NN	denotes	BRCA2
T2445	9034-9041	NN	denotes	protein
T2447	9042-9051	VBN	denotes	expressed
T2448	9052-9054	IN	denotes	by
T2449	9055-9060	NN	denotes	CAPAN
T2451	9060-9061	HYPH	denotes	-
T2452	9061-9062	CD	denotes	1
T2450	9063-9068	NNS	denotes	cells
T2453	9069-9072	VBD	denotes	was
T2439	9073-9085	VBN	denotes	mislocalized
T2454	9086-9088	IN	denotes	to
T2455	9089-9092	DT	denotes	the
T2456	9093-9102	NN	denotes	cytoplasm
T2457	9102-9104	,	denotes	,
T2458	9104-9114	JJ	denotes	consistent
T2459	9115-9119	IN	denotes	with
T2460	9120-9123	DT	denotes	the
T2461	9124-9128	NN	denotes	fact
T2462	9129-9133	IN	denotes	that
T2464	9134-9137	DT	denotes	the
T2466	9138-9148	NN	denotes	truncation
T2465	9149-9156	NN	denotes	protein
T2463	9157-9162	VBZ	denotes	lacks
T2467	9163-9164	DT	denotes	a
T2469	9165-9172	JJ	denotes	nuclear
T2470	9173-9185	NN	denotes	localization
T2468	9186-9192	NN	denotes	signal
T2471	9193-9194	-LRB-	denotes	[
T2472	9194-9196	CD	denotes	28
T2473	9196-9197	-RRB-	denotes	]
T2474	9197-9198	.	denotes	.
T2475	9198-9370	sentence	denotes	Davies et al showed CAPAN-1 cells to be defective in nuclear localization of RAD51, raising the possibility that RAD51 is normally carried to the nucleus by binding BRCA2.
T2476	9199-9205	NNP	denotes	Davies
T2478	9206-9208	FW	denotes	et
T2479	9209-9211	FW	denotes	al
T2477	9212-9218	VBD	denotes	showed
T2480	9219-9224	NN	denotes	CAPAN
T2482	9224-9225	HYPH	denotes	-
T2483	9225-9226	CD	denotes	1
T2481	9227-9232	NNS	denotes	cells
T2485	9233-9235	TO	denotes	to
T2484	9236-9238	VB	denotes	be
T2486	9239-9248	JJ	denotes	defective
T2487	9249-9251	IN	denotes	in
T2488	9252-9259	JJ	denotes	nuclear
T2489	9260-9272	NN	denotes	localization
T2490	9273-9275	IN	denotes	of
T2491	9276-9281	NN	denotes	RAD51
T2492	9281-9283	,	denotes	,
T2493	9283-9290	VBG	denotes	raising
T2494	9291-9294	DT	denotes	the
T2495	9295-9306	NN	denotes	possibility
T2496	9307-9311	IN	denotes	that
T2498	9312-9317	NN	denotes	RAD51
T2499	9318-9320	VBZ	denotes	is
T2500	9321-9329	RB	denotes	normally
T2497	9330-9337	VBN	denotes	carried
T2501	9338-9340	IN	denotes	to
T2502	9341-9344	DT	denotes	the
T2503	9345-9352	NN	denotes	nucleus
T2504	9353-9355	IN	denotes	by
T2505	9356-9363	VBG	denotes	binding
T2506	9364-9369	NN	denotes	BRCA2
T2507	9369-9370	.	denotes	.
T2508	9370-9563	sentence	denotes	It is possible that RAD51 may alternatively be capable of BRCA2-independent transport, but mislocalization of BRCA2 to the cytoplasm may sequester RAD51 and block its normal mode of transport.
T2509	9371-9373	PRP	denotes	It
T2510	9374-9376	VBZ	denotes	is
T2511	9377-9385	JJ	denotes	possible
T2512	9386-9390	IN	denotes	that
T2514	9391-9396	NN	denotes	RAD51
T2515	9397-9400	MD	denotes	may
T2516	9401-9414	RB	denotes	alternatively
T2513	9415-9417	VB	denotes	be
T2517	9418-9425	JJ	denotes	capable
T2518	9426-9428	IN	denotes	of
T2519	9429-9434	NN	denotes	BRCA2
T2521	9434-9435	HYPH	denotes	-
T2520	9435-9446	JJ	denotes	independent
T2522	9447-9456	NN	denotes	transport
T2523	9456-9458	,	denotes	,
T2524	9458-9461	CC	denotes	but
T2525	9462-9477	NN	denotes	mislocalization
T2527	9478-9480	IN	denotes	of
T2528	9481-9486	NN	denotes	BRCA2
T2529	9487-9489	IN	denotes	to
T2530	9490-9493	DT	denotes	the
T2531	9494-9503	NN	denotes	cytoplasm
T2532	9504-9507	MD	denotes	may
T2526	9508-9517	VB	denotes	sequester
T2533	9518-9523	NN	denotes	RAD51
T2534	9524-9527	CC	denotes	and
T2535	9528-9533	VB	denotes	block
T2536	9534-9537	PRP$	denotes	its
T2538	9538-9544	JJ	denotes	normal
T2537	9545-9549	NN	denotes	mode
T2539	9550-9552	IN	denotes	of
T2540	9553-9562	NN	denotes	transport
T2541	9562-9563	.	denotes	.
T2542	9563-9738	sentence	denotes	Support for the hypothesis that the observed mislocalization of RAD51 is an indirect consequence of the BRCA2 defect requires reintroduction of functional BRCA2 into CAPAN-1.
T2543	9564-9571	NN	denotes	Support
T2545	9572-9575	IN	denotes	for
T2546	9576-9579	DT	denotes	the
T2547	9580-9590	NN	denotes	hypothesis
T2548	9591-9595	IN	denotes	that
T2550	9596-9599	DT	denotes	the
T2552	9600-9608	VBN	denotes	observed
T2551	9609-9624	NN	denotes	mislocalization
T2553	9625-9627	IN	denotes	of
T2554	9628-9633	NN	denotes	RAD51
T2549	9634-9636	VBZ	denotes	is
T2555	9637-9639	DT	denotes	an
T2557	9640-9648	JJ	denotes	indirect
T2556	9649-9660	NN	denotes	consequence
T2558	9661-9663	IN	denotes	of
T2559	9664-9667	DT	denotes	the
T2561	9668-9673	NN	denotes	BRCA2
T2560	9674-9680	NN	denotes	defect
T2544	9681-9689	VBZ	denotes	requires
T2562	9690-9704	NN	denotes	reintroduction
T2563	9705-9707	IN	denotes	of
T2564	9708-9718	JJ	denotes	functional
T2565	9719-9724	NN	denotes	BRCA2
T2566	9725-9729	IN	denotes	into
T2567	9730-9735	NN	denotes	CAPAN
T2568	9735-9736	HYPH	denotes	-
T2569	9736-9737	CD	denotes	1
T2570	9737-9738	.	denotes	.
T2571	9738-9935	sentence	denotes	Given the elaborate nature of the RAD51–BRCA2 interaction, it seems somewhat unlikely that the only role of BRCA2 in promoting recombination is to serve as a specific nuclear transporter of RAD51.
T2572	9739-9744	VBN	denotes	Given
T2574	9745-9748	DT	denotes	the
T2576	9749-9758	JJ	denotes	elaborate
T2575	9759-9765	NN	denotes	nature
T2577	9766-9768	IN	denotes	of
T2578	9769-9772	DT	denotes	the
T2580	9773-9778	NN	denotes	RAD51
T2582	9778-9779	HYPH	denotes	–
T2581	9779-9784	NN	denotes	BRCA2
T2579	9785-9796	NN	denotes	interaction
T2583	9796-9798	,	denotes	,
T2584	9798-9800	PRP	denotes	it
T2573	9801-9806	VBZ	denotes	seems
T2585	9807-9815	RB	denotes	somewhat
T2586	9816-9824	JJ	denotes	unlikely
T2587	9825-9829	IN	denotes	that
T2589	9830-9833	DT	denotes	the
T2591	9834-9838	JJ	denotes	only
T2590	9839-9843	NN	denotes	role
T2592	9844-9846	IN	denotes	of
T2593	9847-9852	NN	denotes	BRCA2
T2594	9853-9855	IN	denotes	in
T2595	9856-9865	VBG	denotes	promoting
T2596	9866-9879	NN	denotes	recombination
T2588	9880-9882	VBZ	denotes	is
T2597	9883-9885	TO	denotes	to
T2598	9886-9891	VB	denotes	serve
T2599	9892-9894	IN	denotes	as
T2600	9895-9896	DT	denotes	a
T2602	9897-9905	JJ	denotes	specific
T2603	9906-9913	JJ	denotes	nuclear
T2601	9914-9925	NN	denotes	transporter
T2604	9926-9928	IN	denotes	of
T2605	9929-9934	NN	denotes	RAD51
T2606	9934-9935	.	denotes	.
T2607	9935-10116	sentence	denotes	An attractive alternative, favored by Davies et al [26], is that the association of RAD51–BRCA2 is required to maintain RAD51 in a form that is in a state of 'readiness' (Fig. 2a).
T2608	9936-9938	DT	denotes	An
T2610	9939-9949	JJ	denotes	attractive
T2609	9950-9961	NN	denotes	alternative
T2612	9961-9963	,	denotes	,
T2613	9963-9970	VBN	denotes	favored
T2614	9971-9973	IN	denotes	by
T2615	9974-9980	NNP	denotes	Davies
T2616	9981-9983	FW	denotes	et
T2617	9984-9986	FW	denotes	al
T2618	9987-9988	-LRB-	denotes	[
T2619	9988-9990	CD	denotes	26
T2620	9990-9991	-RRB-	denotes	]
T2621	9991-9993	,	denotes	,
T2611	9993-9995	VBZ	denotes	is
T2622	9996-10000	IN	denotes	that
T2624	10001-10004	DT	denotes	the
T2625	10005-10016	NN	denotes	association
T2626	10017-10019	IN	denotes	of
T2627	10020-10025	NN	denotes	RAD51
T2629	10025-10026	HYPH	denotes	–
T2628	10026-10031	NN	denotes	BRCA2
T2630	10032-10034	VBZ	denotes	is
T2623	10035-10043	VBN	denotes	required
T2631	10044-10046	TO	denotes	to
T2632	10047-10055	VB	denotes	maintain
T2633	10056-10061	NN	denotes	RAD51
T2634	10062-10064	IN	denotes	in
T2635	10065-10066	DT	denotes	a
T2636	10067-10071	NN	denotes	form
T2637	10072-10076	WDT	denotes	that
T2638	10077-10079	VBZ	denotes	is
T2639	10080-10082	IN	denotes	in
T2640	10083-10084	DT	denotes	a
T2641	10085-10090	NN	denotes	state
T2642	10091-10093	IN	denotes	of
T2643	10094-10095	``	denotes	'
T2644	10095-10104	NN	denotes	readiness
T2645	10104-10105	''	denotes	'
T2646	10106-10107	-LRB-	denotes	(
T2648	10107-10111	NNP	denotes	Fig.
T2647	10112-10114	NNP	denotes	2a
T2649	10114-10115	-RRB-	denotes	)
T2650	10115-10116	.	denotes	.
T2651	10116-10283	sentence	denotes	In the absence of this interaction with BRCA2, RAD51 might exist in a form that is not capable of being recruited into a functional repair complex when damage occurs.
T2652	10117-10119	IN	denotes	In
T2654	10120-10123	DT	denotes	the
T2655	10124-10131	NN	denotes	absence
T2656	10132-10134	IN	denotes	of
T2657	10135-10139	DT	denotes	this
T2658	10140-10151	NN	denotes	interaction
T2659	10152-10156	IN	denotes	with
T2660	10157-10162	NN	denotes	BRCA2
T2661	10162-10164	,	denotes	,
T2662	10164-10169	NN	denotes	RAD51
T2663	10170-10175	MD	denotes	might
T2653	10176-10181	VB	denotes	exist
T2664	10182-10184	IN	denotes	in
T2665	10185-10186	DT	denotes	a
T2666	10187-10191	NN	denotes	form
T2667	10192-10196	WDT	denotes	that
T2668	10197-10199	VBZ	denotes	is
T2669	10200-10203	RB	denotes	not
T2670	10204-10211	JJ	denotes	capable
T2671	10212-10214	IN	denotes	of
T2672	10215-10220	VBG	denotes	being
T2673	10221-10230	VBN	denotes	recruited
T2674	10231-10235	IN	denotes	into
T2675	10236-10237	DT	denotes	a
T2677	10238-10248	JJ	denotes	functional
T2678	10249-10255	NN	denotes	repair
T2676	10256-10263	NN	denotes	complex
T2679	10264-10268	WRB	denotes	when
T2681	10269-10275	NN	denotes	damage
T2680	10276-10282	VBZ	denotes	occurs
T2682	10282-10283	.	denotes	.
T2683	10283-10435	sentence	denotes	For example, previous studies indicated that RAD51 shows little binding preference for single-strand DNA (ssDNA) over double-strand DNA (dsDNA) [3,29].
T2684	10284-10287	IN	denotes	For
T2686	10288-10295	NN	denotes	example
T2687	10295-10297	,	denotes	,
T2688	10297-10305	JJ	denotes	previous
T2689	10306-10313	NNS	denotes	studies
T2685	10314-10323	VBD	denotes	indicated
T2690	10324-10328	IN	denotes	that
T2692	10329-10334	NN	denotes	RAD51
T2691	10335-10340	VBZ	denotes	shows
T2693	10341-10347	JJ	denotes	little
T2695	10348-10355	NN	denotes	binding
T2694	10356-10366	NN	denotes	preference
T2696	10367-10370	IN	denotes	for
T2697	10371-10377	JJ	denotes	single
T2699	10377-10378	HYPH	denotes	-
T2698	10378-10384	NN	denotes	strand
T2700	10385-10388	NN	denotes	DNA
T2701	10389-10390	-LRB-	denotes	(
T2702	10390-10395	NN	denotes	ssDNA
T2703	10395-10396	-RRB-	denotes	)
T2704	10397-10401	IN	denotes	over
T2705	10402-10408	JJ	denotes	double
T2707	10408-10409	HYPH	denotes	-
T2706	10409-10415	NN	denotes	strand
T2708	10416-10419	NN	denotes	DNA
T2709	10420-10421	-LRB-	denotes	(
T2710	10421-10426	NN	denotes	dsDNA
T2711	10426-10427	-RRB-	denotes	)
T2712	10428-10429	-LRB-	denotes	[
T2714	10429-10430	CD	denotes	3
T2715	10430-10431	,	denotes	,
T2713	10431-10433	CD	denotes	29
T2716	10433-10434	-RRB-	denotes	]
T2717	10434-10435	.	denotes	.
T2718	10435-10607	sentence	denotes	This result could mean that regulatory factors, such as BRCA2, are required to suppress RAD51–dsDNA interactions to prevent sequestration of RAD51 in a nonfunctional form.
T2719	10436-10440	DT	denotes	This
T2720	10441-10447	NN	denotes	result
T2722	10448-10453	MD	denotes	could
T2721	10454-10458	VB	denotes	mean
T2723	10459-10463	IN	denotes	that
T2725	10464-10474	JJ	denotes	regulatory
T2726	10475-10482	NNS	denotes	factors
T2727	10482-10484	,	denotes	,
T2728	10484-10488	JJ	denotes	such
T2729	10489-10491	IN	denotes	as
T2730	10492-10497	NN	denotes	BRCA2
T2731	10497-10499	,	denotes	,
T2732	10499-10502	VBP	denotes	are
T2724	10503-10511	VBN	denotes	required
T2733	10512-10514	TO	denotes	to
T2734	10515-10523	VB	denotes	suppress
T2735	10524-10529	NN	denotes	RAD51
T2737	10529-10530	HYPH	denotes	–
T2736	10530-10535	NN	denotes	dsDNA
T2738	10536-10548	NNS	denotes	interactions
T2739	10549-10551	TO	denotes	to
T2740	10552-10559	VB	denotes	prevent
T2741	10560-10573	NN	denotes	sequestration
T2742	10574-10576	IN	denotes	of
T2743	10577-10582	NN	denotes	RAD51
T2744	10583-10585	IN	denotes	in
T2745	10586-10587	DT	denotes	a
T2747	10588-10601	JJ	denotes	nonfunctional
T2746	10602-10606	NN	denotes	form
T2748	10606-10607	.	denotes	.
T2749	10607-10795	sentence	denotes	Alternatively, or in addition, suppression of RAD51–ssDNA interaction might be important for preventing RAD51 from binding to the regions of ssDNA that form during normal DNA replication.
T2750	10608-10621	RB	denotes	Alternatively
T2752	10621-10623	,	denotes	,
T2753	10623-10625	CC	denotes	or
T2751	10626-10628	IN	denotes	in
T2755	10629-10637	NN	denotes	addition
T2756	10637-10639	,	denotes	,
T2757	10639-10650	NN	denotes	suppression
T2758	10651-10653	IN	denotes	of
T2759	10654-10659	NN	denotes	RAD51
T2761	10659-10660	HYPH	denotes	–
T2760	10660-10665	NN	denotes	ssDNA
T2762	10666-10677	NN	denotes	interaction
T2763	10678-10683	MD	denotes	might
T2754	10684-10686	VB	denotes	be
T2764	10687-10696	JJ	denotes	important
T2765	10697-10700	IN	denotes	for
T2766	10701-10711	VBG	denotes	preventing
T2767	10712-10717	NN	denotes	RAD51
T2768	10718-10722	IN	denotes	from
T2769	10723-10730	VBG	denotes	binding
T2770	10731-10733	IN	denotes	to
T2771	10734-10737	DT	denotes	the
T2772	10738-10745	NNS	denotes	regions
T2773	10746-10748	IN	denotes	of
T2774	10749-10754	NN	denotes	ssDNA
T2775	10755-10759	WDT	denotes	that
T2776	10760-10764	VBP	denotes	form
T2777	10765-10771	IN	denotes	during
T2778	10772-10778	JJ	denotes	normal
T2780	10779-10782	NN	denotes	DNA
T2779	10783-10794	NN	denotes	replication
T2781	10794-10795	.	denotes	.
T2782	10795-11063	sentence	denotes	BRCA2 may thus promote RAD51 assembly into recombinational repair complexes via a negative regulatory mechanism (i.e. by blocking RAD51–BRCA2 interaction until damage has occurred and factors required for 'productive' assembly of RAD51 at damaged sites are in place).
T2783	10796-10801	NN	denotes	BRCA2
T2785	10802-10805	MD	denotes	may
T2786	10806-10810	RB	denotes	thus
T2784	10811-10818	VB	denotes	promote
T2787	10819-10824	NN	denotes	RAD51
T2788	10825-10833	NN	denotes	assembly
T2789	10834-10838	IN	denotes	into
T2790	10839-10854	JJ	denotes	recombinational
T2792	10855-10861	NN	denotes	repair
T2791	10862-10871	NNS	denotes	complexes
T2793	10872-10875	IN	denotes	via
T2794	10876-10877	DT	denotes	a
T2796	10878-10886	JJ	denotes	negative
T2797	10887-10897	JJ	denotes	regulatory
T2795	10898-10907	NN	denotes	mechanism
T2798	10908-10909	-LRB-	denotes	(
T2799	10909-10913	FW	denotes	i.e.
T2800	10914-10916	IN	denotes	by
T2801	10917-10925	VBG	denotes	blocking
T2802	10926-10937	NN	denotes	RAD51–BRCA2
T2803	10938-10949	NN	denotes	interaction
T2804	10950-10955	IN	denotes	until
T2806	10956-10962	NN	denotes	damage
T2807	10963-10966	VBZ	denotes	has
T2805	10967-10975	VBN	denotes	occurred
T2808	10976-10979	CC	denotes	and
T2809	10980-10987	NNS	denotes	factors
T2811	10988-10996	VBN	denotes	required
T2812	10997-11000	IN	denotes	for
T2813	11001-11002	``	denotes	'
T2814	11002-11012	JJ	denotes	productive
T2816	11012-11013	''	denotes	'
T2815	11014-11022	NN	denotes	assembly
T2817	11023-11025	IN	denotes	of
T2818	11026-11031	NN	denotes	RAD51
T2819	11032-11034	IN	denotes	at
T2820	11035-11042	JJ	denotes	damaged
T2821	11043-11048	NNS	denotes	sites
T2810	11049-11052	VBP	denotes	are
T2822	11053-11055	IN	denotes	in
T2823	11056-11061	NN	denotes	place
T2824	11061-11062	-RRB-	denotes	)
T2825	11062-11063	.	denotes	.
T2826	11063-11250	sentence	denotes	As mentioned previously, the ability of a BRC peptide to repress the RAD51–BRCA2 interaction could reflect a role of full-length protein in suppressing unwanted RAD51–BRCA2 interactions.
T2827	11064-11066	IN	denotes	As
T2828	11067-11076	VBN	denotes	mentioned
T2830	11077-11087	RB	denotes	previously
T2831	11087-11089	,	denotes	,
T2832	11089-11092	DT	denotes	the
T2833	11093-11100	NN	denotes	ability
T2834	11101-11103	IN	denotes	of
T2835	11104-11105	DT	denotes	a
T2837	11106-11109	NN	denotes	BRC
T2836	11110-11117	NN	denotes	peptide
T2838	11118-11120	TO	denotes	to
T2839	11121-11128	VB	denotes	repress
T2840	11129-11132	DT	denotes	the
T2842	11133-11138	NN	denotes	RAD51
T2843	11138-11139	HYPH	denotes	–
T2844	11139-11144	NN	denotes	BRCA2
T2841	11145-11156	NN	denotes	interaction
T2845	11157-11162	MD	denotes	could
T2829	11163-11170	VB	denotes	reflect
T2846	11171-11172	DT	denotes	a
T2847	11173-11177	NN	denotes	role
T2848	11178-11180	IN	denotes	of
T2849	11181-11185	JJ	denotes	full
T2851	11185-11186	HYPH	denotes	-
T2850	11186-11192	NN	denotes	length
T2852	11193-11200	NN	denotes	protein
T2853	11201-11203	IN	denotes	in
T2854	11204-11215	VBG	denotes	suppressing
T2855	11216-11224	JJ	denotes	unwanted
T2857	11225-11230	NN	denotes	RAD51
T2859	11230-11231	HYPH	denotes	–
T2858	11231-11236	NN	denotes	BRCA2
T2856	11237-11249	NNS	denotes	interactions
T2860	11249-11250	.	denotes	.
T2861	11250-12442	sentence	denotes	Figure 2 Potential roles of BRCA2 in promoting assembly of Rad51 at sites of DNA damage. Chromosomal DNA is shown as pairs of straight lines, Rad51 as open circles, and BRCA2 as grey bars. (a) Prevention of nonproductive DNA interactions. BRCA2–Rad51 interaction is proposed to suppress RAD51–BRCA2 interactions until DNA damage is present. When damage occurs, Rad51 is recruited to damaged sites where is polymerizes into nucleoprotein filaments. In this model, BRCA2 is not required for assembly of functional complexes at damaged sites, only to prevent a substantial fraction of Rad51 from being sequestered in a nonfunctional form. In a BRCA2-defective cell, mutant Rad51 becomes associated with DNA at random sites and is therefore not readily recruited to sites of damage. (b) Positive regulation. BRCA2 is proposed to be required for Rad51 to assemble into functional recombinational repair complexes at sites of damage. In BRCA-defective cells, Rad51 fails to associate with sites of damage due to lack of an assembly factor. Yet another potential role for BRCA2 in promoting RAD51-dependent recombinational repair is a positive role in assembly of RAD51 at damaged sites (Fig. 2b).
T2862	12286-12289	RB	denotes	Yet
T2864	12290-12297	DT	denotes	another
T2865	12298-12307	JJ	denotes	potential
T2863	12308-12312	NN	denotes	role
T2867	12313-12316	IN	denotes	for
T2868	12317-12322	NN	denotes	BRCA2
T2869	12323-12325	IN	denotes	in
T2870	12326-12335	VBG	denotes	promoting
T2871	12336-12341	NN	denotes	RAD51
T2873	12341-12342	HYPH	denotes	-
T2872	12342-12351	JJ	denotes	dependent
T2875	12352-12367	JJ	denotes	recombinational
T2874	12368-12374	NN	denotes	repair
T2866	12375-12377	VBZ	denotes	is
T2876	12378-12379	DT	denotes	a
T2878	12380-12388	JJ	denotes	positive
T2877	12389-12393	NN	denotes	role
T2879	12394-12396	IN	denotes	in
T2880	12397-12405	NN	denotes	assembly
T2881	12406-12408	IN	denotes	of
T2882	12409-12414	NN	denotes	RAD51
T2883	12415-12417	IN	denotes	at
T2884	12418-12425	VBN	denotes	damaged
T2885	12426-12431	NNS	denotes	sites
T2886	12432-12433	-LRB-	denotes	(
T2888	12433-12437	NN	denotes	Fig.
T2887	12438-12440	NN	denotes	2b
T2889	12440-12441	-RRB-	denotes	)
T2890	12441-12442	.	denotes	.
T2891	12442-12570	sentence	denotes	Several proteins that interact with RAD51 are thought to 'mediate' assembly of RAD51 at sites of DNA damage (reviewed in [30]).
T2892	12443-12450	JJ	denotes	Several
T2893	12451-12459	NN	denotes	proteins
T2895	12460-12464	WDT	denotes	that
T2896	12465-12473	VBP	denotes	interact
T2897	12474-12478	IN	denotes	with
T2898	12479-12484	NN	denotes	RAD51
T2899	12485-12488	VBP	denotes	are
T2894	12489-12496	VBN	denotes	thought
T2900	12497-12499	TO	denotes	to
T2902	12500-12501	``	denotes	'
T2901	12501-12508	VB	denotes	mediate
T2903	12508-12509	''	denotes	'
T2904	12510-12518	NN	denotes	assembly
T2905	12519-12521	IN	denotes	of
T2906	12522-12527	NN	denotes	RAD51
T2907	12528-12530	IN	denotes	at
T2908	12531-12536	NNS	denotes	sites
T2909	12537-12539	IN	denotes	of
T2910	12540-12543	NN	denotes	DNA
T2911	12544-12550	NN	denotes	damage
T2912	12551-12552	-LRB-	denotes	(
T2913	12552-12560	VBN	denotes	reviewed
T2914	12561-12563	IN	denotes	in
T2915	12564-12565	-LRB-	denotes	[
T2916	12565-12567	CD	denotes	30
T2917	12567-12568	-RRB-	denotes	]
T2918	12568-12569	-RRB-	denotes	)
T2919	12569-12570	.	denotes	.
T2920	12570-12721	sentence	denotes	RAD51 paralogs in particular, including XRCC2, XRCC3, RAD51B, RAD51C and RAD51D, may function as a complex that actively promotes RAD51 assembly [31].
T2921	12571-12576	NN	denotes	RAD51
T2922	12577-12585	NNS	denotes	paralogs
T2924	12586-12588	IN	denotes	in
T2925	12589-12599	JJ	denotes	particular
T2926	12599-12601	,	denotes	,
T2927	12601-12610	VBG	denotes	including
T2928	12611-12616	NN	denotes	XRCC2
T2929	12616-12618	,	denotes	,
T2930	12618-12623	NN	denotes	XRCC3
T2931	12623-12625	,	denotes	,
T2932	12625-12631	NN	denotes	RAD51B
T2933	12631-12633	,	denotes	,
T2934	12633-12639	NN	denotes	RAD51C
T2935	12640-12643	CC	denotes	and
T2936	12644-12650	NN	denotes	RAD51D
T2937	12650-12652	,	denotes	,
T2938	12652-12655	MD	denotes	may
T2923	12656-12664	VB	denotes	function
T2939	12665-12667	IN	denotes	as
T2940	12668-12669	DT	denotes	a
T2941	12670-12677	NN	denotes	complex
T2942	12678-12682	WDT	denotes	that
T2944	12683-12691	RB	denotes	actively
T2943	12692-12700	VBZ	denotes	promotes
T2945	12701-12706	NN	denotes	RAD51
T2946	12707-12715	NN	denotes	assembly
T2947	12716-12717	-LRB-	denotes	[
T2948	12717-12719	CD	denotes	31
T2949	12719-12720	-RRB-	denotes	]
T2950	12720-12721	.	denotes	.
T2951	12721-12841	sentence	denotes	BRCA2 could cooperate with RAD51 paralogs in promoting RAD51 assembly, or even provide an alternative assembly pathway.
T2952	12722-12727	NN	denotes	BRCA2
T2954	12728-12733	MD	denotes	could
T2953	12734-12743	VB	denotes	cooperate
T2955	12744-12748	IN	denotes	with
T2956	12749-12754	NN	denotes	RAD51
T2957	12755-12763	NNS	denotes	paralogs
T2958	12764-12766	IN	denotes	in
T2959	12767-12776	VBG	denotes	promoting
T2960	12777-12782	NN	denotes	RAD51
T2961	12783-12791	NN	denotes	assembly
T2962	12791-12793	,	denotes	,
T2963	12793-12795	CC	denotes	or
T2964	12796-12800	RB	denotes	even
T2965	12801-12808	VB	denotes	provide
T2966	12809-12811	DT	denotes	an
T2968	12812-12823	JJ	denotes	alternative
T2969	12824-12832	NN	denotes	assembly
T2967	12833-12840	NN	denotes	pathway
T2970	12840-12841	.	denotes	.
T2971	12841-13041	sentence	denotes	The observation that BRCA2 localizes to damage-induced subnuclear foci with RAD51 seems consistent with the possibility that BRCA2 plays a positive role in assembly of the recombination complex [11].
T2972	12842-12845	DT	denotes	The
T2973	12846-12857	NN	denotes	observation
T2975	12858-12862	IN	denotes	that
T2977	12863-12868	NN	denotes	BRCA2
T2976	12869-12878	VBZ	denotes	localizes
T2978	12879-12881	IN	denotes	to
T2979	12882-12888	NN	denotes	damage
T2981	12888-12889	HYPH	denotes	-
T2980	12889-12896	VBN	denotes	induced
T2983	12897-12907	JJ	denotes	subnuclear
T2982	12908-12912	NNS	denotes	foci
T2984	12913-12917	IN	denotes	with
T2985	12918-12923	NN	denotes	RAD51
T2974	12924-12929	VBZ	denotes	seems
T2986	12930-12940	JJ	denotes	consistent
T2987	12941-12945	IN	denotes	with
T2988	12946-12949	DT	denotes	the
T2989	12950-12961	NN	denotes	possibility
T2990	12962-12966	IN	denotes	that
T2992	12967-12972	NN	denotes	BRCA2
T2991	12973-12978	VBZ	denotes	plays
T2993	12979-12980	DT	denotes	a
T2995	12981-12989	JJ	denotes	positive
T2994	12990-12994	NN	denotes	role
T2996	12995-12997	IN	denotes	in
T2997	12998-13006	NN	denotes	assembly
T2998	13007-13009	IN	denotes	of
T2999	13010-13013	DT	denotes	the
T3001	13014-13027	NN	denotes	recombination
T3000	13028-13035	NN	denotes	complex
T3002	13036-13037	-LRB-	denotes	[
T3003	13037-13039	CD	denotes	11
T3004	13039-13040	-RRB-	denotes	]
T3005	13040-13041	.	denotes	.
T3006	13041-13188	sentence	denotes	Finally, it is important to note that the two models for the function of the BRCA2–RAD51 interaction shown in Figure 2 are not mutually exclusive.
T3007	13042-13049	RB	denotes	Finally
T3009	13049-13051	,	denotes	,
T3010	13051-13053	PRP	denotes	it
T3008	13054-13056	VBZ	denotes	is
T3011	13057-13066	JJ	denotes	important
T3012	13067-13069	TO	denotes	to
T3013	13070-13074	VB	denotes	note
T3014	13075-13079	IN	denotes	that
T3016	13080-13083	DT	denotes	the
T3018	13084-13087	CD	denotes	two
T3017	13088-13094	NNS	denotes	models
T3019	13095-13098	IN	denotes	for
T3020	13099-13102	DT	denotes	the
T3021	13103-13111	NN	denotes	function
T3022	13112-13114	IN	denotes	of
T3023	13115-13118	DT	denotes	the
T3025	13119-13124	NN	denotes	BRCA2
T3027	13124-13125	HYPH	denotes	–
T3026	13125-13130	NN	denotes	RAD51
T3024	13131-13142	NN	denotes	interaction
T3028	13143-13148	VBN	denotes	shown
T3029	13149-13151	IN	denotes	in
T3030	13152-13158	NN	denotes	Figure
T3031	13159-13160	CD	denotes	2
T3015	13161-13164	VBP	denotes	are
T3032	13165-13168	RB	denotes	not
T3033	13169-13177	RB	denotes	mutually
T3034	13178-13187	JJ	denotes	exclusive
T3035	13187-13188	.	denotes	.
T3036	13188-13343	sentence	denotes	BRCA2 could prevent inappropriate RAD51 assembly in the absence of damage and promote functional RAD51 assembly at DNA lesions during the damage response.
T3037	13189-13194	NN	denotes	BRCA2
T3039	13195-13200	MD	denotes	could
T3038	13201-13208	VB	denotes	prevent
T3040	13209-13222	JJ	denotes	inappropriate
T3042	13223-13228	NN	denotes	RAD51
T3041	13229-13237	NN	denotes	assembly
T3043	13238-13240	IN	denotes	in
T3044	13241-13244	DT	denotes	the
T3045	13245-13252	NN	denotes	absence
T3046	13253-13255	IN	denotes	of
T3047	13256-13262	NN	denotes	damage
T3048	13263-13266	CC	denotes	and
T3049	13267-13274	VB	denotes	promote
T3050	13275-13285	JJ	denotes	functional
T3052	13286-13291	NN	denotes	RAD51
T3051	13292-13300	NN	denotes	assembly
T3053	13301-13303	IN	denotes	at
T3054	13304-13307	NN	denotes	DNA
T3055	13308-13315	NNS	denotes	lesions
T3056	13316-13322	IN	denotes	during
T3057	13323-13326	DT	denotes	the
T3059	13327-13333	NN	denotes	damage
T3058	13334-13342	NN	denotes	response
T3060	13342-13343	.	denotes	.
T3061	13343-13478	sentence	denotes	Further experiments are clearly required to clarify the functional interactions between BRCA2 and RAD51 during recombinational repair.
T3062	13344-13351	JJ	denotes	Further
T3063	13352-13363	NNS	denotes	experiments
T3065	13364-13367	VBP	denotes	are
T3066	13368-13375	RB	denotes	clearly
T3064	13376-13384	VBN	denotes	required
T3067	13385-13387	TO	denotes	to
T3068	13388-13395	VB	denotes	clarify
T3069	13396-13399	DT	denotes	the
T3071	13400-13410	JJ	denotes	functional
T3070	13411-13423	NNS	denotes	interactions
T3072	13424-13431	IN	denotes	between
T3073	13432-13437	NN	denotes	BRCA2
T3074	13438-13441	CC	denotes	and
T3075	13442-13447	NN	denotes	RAD51
T3076	13448-13454	IN	denotes	during
T3077	13455-13470	JJ	denotes	recombinational
T3078	13471-13477	NN	denotes	repair
T3079	13477-13478	.	denotes	.
T3080	13478-13606	sentence	denotes	It should be relatively straightforward to determine whether the role of BRCA2 is limited to transporting RAD51 to the nucleus.
T3081	13479-13481	PRP	denotes	It
T3083	13482-13488	MD	denotes	should
T3082	13489-13491	VB	denotes	be
T3084	13492-13502	RB	denotes	relatively
T3085	13503-13518	JJ	denotes	straightforward
T3086	13519-13521	TO	denotes	to
T3087	13522-13531	VB	denotes	determine
T3088	13532-13539	IN	denotes	whether
T3090	13540-13543	DT	denotes	the
T3091	13544-13548	NN	denotes	role
T3092	13549-13551	IN	denotes	of
T3093	13552-13557	NN	denotes	BRCA2
T3094	13558-13560	VBZ	denotes	is
T3089	13561-13568	VBN	denotes	limited
T3095	13569-13571	IN	denotes	to
T3096	13572-13584	VBG	denotes	transporting
T3097	13585-13590	NN	denotes	RAD51
T3098	13591-13593	IN	denotes	to
T3099	13594-13597	DT	denotes	the
T3100	13598-13605	NN	denotes	nucleus
T3101	13605-13606	.	denotes	.
T3102	13606-13760	sentence	denotes	If this were the case, addition of a nuclear localization signal to RAD51 should suppress the RAD51-mediated phenotypes associated with a BRCA2 mutation.
T3103	13607-13609	IN	denotes	If
T3105	13610-13614	DT	denotes	this
T3104	13615-13619	VBD	denotes	were
T3107	13620-13623	DT	denotes	the
T3108	13624-13628	NN	denotes	case
T3109	13628-13630	,	denotes	,
T3110	13630-13638	NN	denotes	addition
T3111	13639-13641	IN	denotes	of
T3112	13642-13643	DT	denotes	a
T3114	13644-13651	JJ	denotes	nuclear
T3115	13652-13664	NN	denotes	localization
T3113	13665-13671	NN	denotes	signal
T3116	13672-13674	IN	denotes	to
T3117	13675-13680	NN	denotes	RAD51
T3118	13681-13687	MD	denotes	should
T3106	13688-13696	VB	denotes	suppress
T3119	13697-13700	DT	denotes	the
T3121	13701-13706	NN	denotes	RAD51
T3123	13706-13707	HYPH	denotes	-
T3122	13707-13715	VBN	denotes	mediated
T3120	13716-13726	NNS	denotes	phenotypes
T3124	13727-13737	VBN	denotes	associated
T3125	13738-13742	IN	denotes	with
T3126	13743-13744	DT	denotes	a
T3128	13745-13750	NN	denotes	BRCA2
T3127	13751-13759	NN	denotes	mutation
T3129	13759-13760	.	denotes	.
T3130	13760-13887	sentence	denotes	However, biochemical experiments are needed to determine whether full-length BRCA2 blocks or promotes RAD51–BRCA2 interaction.
T3131	13761-13768	RB	denotes	However
T3133	13768-13770	,	denotes	,
T3134	13770-13781	JJ	denotes	biochemical
T3135	13782-13793	NNS	denotes	experiments
T3136	13794-13797	VBP	denotes	are
T3132	13798-13804	VBN	denotes	needed
T3137	13805-13807	TO	denotes	to
T3138	13808-13817	VB	denotes	determine
T3139	13818-13825	IN	denotes	whether
T3141	13826-13830	JJ	denotes	full
T3143	13830-13831	HYPH	denotes	-
T3142	13831-13837	NN	denotes	length
T3144	13838-13843	NN	denotes	BRCA2
T3140	13844-13850	VBZ	denotes	blocks
T3145	13851-13853	CC	denotes	or
T3146	13854-13862	VBZ	denotes	promotes
T3147	13863-13868	NN	denotes	RAD51
T3149	13868-13869	HYPH	denotes	–
T3148	13869-13874	NN	denotes	BRCA2
T3150	13875-13886	NN	denotes	interaction
T3151	13886-13887	.	denotes	.
T3152	13887-13976	sentence	denotes	Such experiments could also reveal additional roles for BRCA2 in recombinational repair.
T3153	13888-13892	JJ	denotes	Such
T3154	13893-13904	NNS	denotes	experiments
T3156	13905-13910	MD	denotes	could
T3157	13911-13915	RB	denotes	also
T3155	13916-13922	VB	denotes	reveal
T3158	13923-13933	JJ	denotes	additional
T3159	13934-13939	NNS	denotes	roles
T3160	13940-13943	IN	denotes	for
T3161	13944-13949	NN	denotes	BRCA2
T3162	13950-13952	IN	denotes	in
T3163	13953-13968	JJ	denotes	recombinational
T3164	13969-13975	NN	denotes	repair
T3165	13975-13976	.	denotes	.
T3166	13976-14123	sentence	denotes	However, several technical obstacles, including the large size of BRCA2, promise to make biochemical characterization of BRCA2 function difficult.
T3167	13977-13984	RB	denotes	However
T3169	13984-13986	,	denotes	,
T3170	13986-13993	JJ	denotes	several
T3172	13994-14003	JJ	denotes	technical
T3171	14004-14013	NNS	denotes	obstacles
T3173	14013-14015	,	denotes	,
T3174	14015-14024	VBG	denotes	including
T3175	14025-14028	DT	denotes	the
T3177	14029-14034	JJ	denotes	large
T3176	14035-14039	NN	denotes	size
T3178	14040-14042	IN	denotes	of
T3179	14043-14048	NN	denotes	BRCA2
T3180	14048-14050	,	denotes	,
T3168	14050-14057	VBP	denotes	promise
T3181	14058-14060	TO	denotes	to
T3182	14061-14065	VB	denotes	make
T3183	14066-14077	JJ	denotes	biochemical
T3184	14078-14094	NN	denotes	characterization
T3186	14095-14097	IN	denotes	of
T3187	14098-14103	NN	denotes	BRCA2
T3188	14104-14112	NN	denotes	function
T3185	14113-14122	JJ	denotes	difficult
T3189	14122-14123	.	denotes	.
R10	T48	T46	dobj	evidence,provide
R100	T146	T144	relcl	carried,context
R1000	T1467	T1469	compound	length,BRCA2
R1001	T1468	T1467	punct	-,length
R1002	T1469	T1465	dobj	BRCA2,expressing
R1003	T1470	T1471	punct	(,was
R1004	T1471	T1469	parataxis	was,BRCA2
R1005	T1472	T1473	det	the,defect
R1006	T1473	T1471	nsubj	defect,was
R1007	T1474	T1473	acl	observed,defect
R1008	T1475	T1476	advmod	about,sixfold
R1009	T1476	T1471	advmod	sixfold,was
R101	T147	T145	pobj	which,in
R1010	T1477	T1476	advmod	fivefold,sixfold
R1011	T1478	T1476	quantmod	to,sixfold
R1012	T1479	T1471	punct	),was
R1013	T1480	T1455	punct	.,found
R1014	T1482	T1483	advmod	Second,was
R1015	T1484	T1483	punct	", ",was
R1016	T1485	T1483	prep	in,was
R1017	T1486	T1487	det	an,study
R1018	T1487	T1485	pobj	study,in
R1019	T1488	T1487	amod	independent,study
R102	T148	T149	det	the,studies
R1020	T1489	T1483	punct	", ",was
R1021	T1490	T1483	nsubj	Powell,was
R1022	T1491	T1483	acomp	able,was
R1023	T1492	T1493	aux	to,compare
R1024	T1493	T1491	xcomp	compare,able
R1025	T1494	T1493	dobj	derivatives,compare
R1026	T1495	T1494	prep	of,derivatives
R1027	T1496	T1495	pobj	CAPAN,of
R1028	T1497	T1496	punct	-,CAPAN
R1029	T1498	T1496	nummod	1,CAPAN
R103	T149	T146	nsubjpass	studies,carried
R1030	T1499	T1500	dep	that,differed
R1031	T1500	T1494	relcl	differed,derivatives
R1032	T1501	T1500	advmod	only,differed
R1033	T1502	T1500	prep	in,differed
R1034	T1503	T1504	poss	their,ability
R1035	T1504	T1502	pobj	ability,in
R1036	T1505	T1506	aux	to,express
R1037	T1506	T1504	acl	express,ability
R1038	T1507	T1508	amod	full,length
R1039	T1508	T1510	compound	length,protein
R104	T150	T149	nummod	two,studies
R1040	T1509	T1508	punct	-,length
R1041	T1510	T1506	dobj	protein,express
R1042	T1511	T1510	compound	BRCA2,protein
R1043	T1512	T1513	punct	(,Powell
R1044	T1513	T1493	meta	Powell,compare
R1045	T1514	T1513	compound	S.,Powell
R1046	T1515	T1513	punct	", ",Powell
R1047	T1516	T1517	amod	personal,communication
R1048	T1517	T1513	dep	communication,Powell
R1049	T1518	T1513	punct	", ",Powell
R105	T151	T146	auxpass	were,carried
R1050	T1519	T1513	npadvmod	2001,Powell
R1051	T1520	T1513	punct	),Powell
R1052	T1521	T1483	punct	.,was
R1053	T1523	T1524	prep	In,yielded
R1054	T1525	T1526	det	these,experiments
R1055	T1526	T1523	pobj	experiments,In
R1056	T1527	T1524	punct	", ",yielded
R1057	T1528	T1529	det	the,derivative
R1058	T1529	T1524	nsubj	derivative,yielded
R1059	T1530	T1529	acl	expressing,derivative
R106	T152	T146	prt	out,carried
R1060	T1531	T1532	amod	full,length
R1061	T1532	T1534	compound	length,BRCA2
R1062	T1533	T1532	punct	-,length
R1063	T1534	T1530	dobj	BRCA2,expressing
R1064	T1535	T1536	advmod	10-fold,more
R1065	T1536	T1537	amod	more,recombinants
R1066	T1537	T1524	dobj	recombinants,yielded
R1067	T1538	T1537	prep	than,recombinants
R1068	T1539	T1538	pobj	controls,than
R1069	T1540	T1539	acl	expressing,controls
R107	T153	T141	punct	", ",review
R1070	T1541	T1542	advmod	only,BRCA2
R1071	T1542	T1540	dobj	BRCA2,expressing
R1072	T1543	T1542	amod	truncated,BRCA2
R1073	T1544	T1545	punct	(,Powell
R1074	T1545	T1524	meta	Powell,yielded
R1075	T1546	T1545	compound	S.,Powell
R1076	T1547	T1545	punct	", ",Powell
R1077	T1548	T1549	amod	personal,communication
R1078	T1549	T1545	dep	communication,Powell
R1079	T1550	T1545	punct	", ",Powell
R108	T154	T155	nsubj	we,comment
R1080	T1551	T1545	dep	2001,Powell
R1081	T1552	T1545	punct	),Powell
R1082	T1553	T1524	punct	.,yielded
R1083	T1555	T1556	mark	While,was
R1084	T1556	T1563	advcl	was,raise
R1085	T1557	T1558	det	the,design
R1086	T1558	T1556	nsubj	design,was
R1087	T1559	T1558	amod	experimental,design
R1088	T1560	T1558	prep	of,design
R1089	T1561	T1562	det	these,experiments
R109	T155	T141	conj	comment,review
R1090	T1562	T1560	pobj	experiments,of
R1091	T1564	T1565	advmod	somewhat,different
R1092	T1565	T1556	acomp	different,was
R1093	T1566	T1565	prep	from,different
R1094	T1567	T1566	pobj	that,from
R1095	T1568	T1567	prep	of,that
R1096	T1569	T1570	compound	Moyna,han
R1097	T1570	T1568	pobj	han,of
R1098	T1571	T1570	punct	-,han
R1099	T1572	T1573	advmod	et,al
R11	T49	T48	amod	relevant,evidence
R110	T156	T155	prep	on,comment
R1100	T1573	T1570	advmod	al,han
R1101	T1574	T1575	punct	[,24
R1102	T1575	T1570	parataxis	24,han
R1103	T1576	T1575	punct	],24
R1104	T1577	T1563	punct	", ",raise
R1105	T1578	T1579	det	the,results
R1106	T1579	T1563	nsubj	results,raise
R1107	T1580	T1581	det	the,possibility
R1108	T1581	T1563	dobj	possibility,raise
R1109	T1582	T1583	mark	that,have
R111	T157	T158	det	the,results
R1110	T1583	T1581	acl	have,possibility
R1111	T1584	T1585	nmod	CAPAN,cells
R1112	T1585	T1583	nsubj	cells,have
R1113	T1586	T1584	punct	-,CAPAN
R1114	T1587	T1584	nummod	1,CAPAN
R1115	T1588	T1589	amod	more,one
R1116	T1589	T1591	nummod	one,mutation
R1117	T1590	T1589	quantmod	than,one
R1118	T1591	T1583	dobj	mutation,have
R1119	T1592	T1593	dep	that,lowers
R112	T158	T156	pobj	results,on
R1120	T1593	T1591	relcl	lowers,mutation
R1121	T1594	T1595	det	the,efficiency
R1122	T1595	T1593	dobj	efficiency,lowers
R1123	T1596	T1595	prep	of,efficiency
R1124	T1597	T1598	amod	recombinational,repair
R1125	T1598	T1596	pobj	repair,of
R1126	T1599	T1598	amod	relative,repair
R1127	T1600	T1599	prep	to,relative
R1128	T1601	T1600	pobj	that,to
R1129	T1602	T1601	acl	observed,that
R113	T159	T158	acl	presented,results
R1130	T1603	T1602	prep	in,observed
R1131	T1604	T1605	amod	other,lines
R1132	T1605	T1603	pobj	lines,in
R1133	T1606	T1605	amod	human,lines
R1134	T1607	T1605	compound	cell,lines
R1135	T1608	T1563	punct	.,raise
R1136	T1610	T1611	advmod	Conversely,be
R1137	T1612	T1611	punct	", ",be
R1138	T1613	T1614	det	the,defect
R1139	T1614	T1611	nsubj	defect,be
R114	T160	T155	punct	", ",comment
R1140	T1615	T1614	compound	BRCA2,defect
R1141	T1616	T1614	prep	in,defect
R1142	T1617	T1616	pobj	CAPAN,in
R1143	T1618	T1617	punct	-,CAPAN
R1144	T1619	T1617	nummod	1,CAPAN
R1145	T1621	T1622	advmod	fully,responsible
R1146	T1622	T1611	acomp	responsible,be
R1147	T1623	T1622	prep	for,responsible
R1148	T1624	T1625	det	the,defect
R1149	T1625	T1623	pobj	defect,for
R115	T161	T155	cc	and,comment
R1150	T1620	T1611	aux	could,be
R1151	T1627	T1625	prep	in,defect
R1152	T1628	T1627	pobj	recombination,in
R1153	T1626	T1625	amod	100-fold,defect
R1154	T1630	T1611	advcl	was,be
R1155	T1631	T1632	det	the,level
R1156	T1629	T1630	mark	if,was
R1157	T1633	T1632	prep	of,level
R1158	T1634	T1635	compound	BRCA2,complementation
R1159	T1632	T1630	nsubj	level,was
R116	T162	T163	nsubj	we,discuss
R1160	T1636	T1632	acl	observed,level
R1161	T1637	T1636	agent	by,observed
R1162	T1638	T1637	pobj	Powell,by
R1163	T1639	T1630	acomp	incomplete,was
R1164	T1635	T1633	pobj	complementation,of
R1165	T1642	T1643	advmod	Furthermore,accounted
R1166	T1640	T1611	punct	.,be
R1167	T1644	T1643	punct	", ",accounted
R1168	T1645	T1646	det	the,discrepancy
R1169	T1647	T1646	prep	between,discrepancy
R117	T163	T155	conj	discuss,comment
R1170	T1648	T1649	det	the,difference
R1171	T1646	T1643	nsubjpass	discrepancy,accounted
R1172	T1650	T1649	amod	100-fold,difference
R1173	T1651	T1649	prep	between,difference
R1174	T1649	T1647	pobj	difference,between
R1175	T1653	T1652	punct	-,CAPAN
R1176	T1654	T1652	nummod	1,CAPAN
R1177	T1652	T1651	pobj	CAPAN,between
R1178	T1656	T1657	det	the,lines
R1179	T1657	T1652	conj	lines,CAPAN
R118	T164	T163	dobj	models,discuss
R1180	T1655	T1652	cc	and,CAPAN
R1181	T1659	T1657	amod	human,lines
R1182	T1660	T1646	prep	as,discrepancy
R1183	T1658	T1657	amod	other,lines
R1184	T1662	T1661	prep	with,compared
R1185	T1661	T1660	prep	compared,as
R1186	T1664	T1662	pobj	difference,with
R1187	T1663	T1664	det	the,difference
R1188	T1666	T1664	nummod	sixfold,difference
R1189	T1665	T1666	amod	fivefold,sixfold
R119	T165	T164	acl	designed,models
R1190	T1668	T1664	prep	between,difference
R1191	T1669	T1670	det	the,cells
R1192	T1667	T1666	quantmod	to,sixfold
R1193	T1671	T1672	compound	Brca2lex1,lex2
R1194	T1672	T1670	compound	lex2,cells
R1195	T1670	T1668	pobj	cells,between
R1196	T1674	T1670	cc	and,cells
R1197	T1675	T1676	nmod	Brca2,cells
R1198	T1673	T1672	punct	/,lex2
R1199	T1677	T1675	punct	+,Brca2
R12	T50	T49	prep	to,relevant
R120	T166	T167	aux	to,account
R1200	T1678	T1675	punct	/,Brca2
R1201	T1679	T1675	punct	+,Brca2
R1202	T1676	T1670	conj	cells,cells
R1203	T1681	T1643	aux	might,accounted
R1204	T1682	T1643	advmod	simply,accounted
R1205	T1680	T1676	compound	ES,cells
R1206	T1684	T1643	prep	for,accounted
R1207	T1685	T1643	prep	by,accounted
R1208	T1683	T1643	auxpass	be,accounted
R1209	T1687	T1685	pobj	fact,by
R121	T167	T165	advcl	account,designed
R1210	T1688	T1689	mark	that,is
R1211	T1686	T1687	det	the,fact
R1212	T1690	T1691	compound	Brca2lex1,lex2
R1213	T1691	T1689	nsubj	lex2,is
R1214	T1689	T1687	acl	is,fact
R1215	T1693	T1689	neg	not,is
R1216	T1694	T1695	det	a,allele
R1217	T1692	T1691	punct	/,lex2
R1218	T1696	T1695	amod	null,allele
R1219	T1697	T1643	punct	.,accounted
R122	T168	T167	prep	for,account
R1220	T1695	T1689	attr	allele,is
R1221	T1699	T1700	amod	Additional,studies
R1222	T1700	T1701	nsubj	studies,are
R1223	T1702	T1701	acomp	likely,are
R1224	T1704	T1702	xcomp	shed,likely
R1225	T1705	T1704	dobj	light,shed
R1226	T1703	T1704	aux	to,shed
R1227	T1707	T1708	det	the,efficiency
R1228	T1708	T1706	pobj	efficiency,on
R1229	T1706	T1704	prep	on,shed
R123	T169	T170	det	the,role
R1230	T1710	T1711	npadvmod	BRCA2,independent
R1231	T1711	T1713	amod	independent,pathways
R1232	T1709	T1708	prep	of,efficiency
R1233	T1713	T1709	pobj	pathways,of
R1234	T1714	T1713	compound	recombination,pathways
R1235	T1712	T1711	punct	-,independent
R1236	T1719	T1717	advmod	together,Taken
R1237	T1715	T1701	punct	.,are
R1238	T1720	T1718	punct	", ",indicate
R1239	T1717	T1718	advcl	Taken,indicate
R124	T170	T168	pobj	role,for
R1240	T1722	T1718	nsubj	results,indicate
R1241	T1721	T1722	det	the,results
R1242	T1724	T1718	ccomp	required,indicate
R1243	T1725	T1724	nsubjpass	BRCA2,required
R1244	T1723	T1724	mark	that,required
R1245	T1727	T1724	advmod	indeed,required
R1246	T1728	T1724	prep	for,required
R1247	T1726	T1724	auxpass	is,required
R1248	T1730	T1728	pobj	levels,for
R1249	T1729	T1730	amod	high,levels
R125	T171	T170	prep	of,role
R1250	T1732	T1733	amod	recombinational,repair
R1251	T1733	T1731	pobj	repair,of
R1252	T1731	T1730	prep	of,levels
R1253	T1735	T1736	preconj	both,mouse
R1254	T1734	T1724	prep	in,required
R1255	T1737	T1736	amod	human,mouse
R1256	T1738	T1736	cc	and,mouse
R1257	T1736	T1734	pobj	mouse,in
R1258	T1739	T1718	punct	.,indicate
R126	T172	T171	pobj	BRCA2,of
R1262	T2154	T2155	det	The,paper
R1263	T2155	T2157	nsubj	paper,is
R1264	T2156	T2155	amod	second,paper
R1265	T2158	T2155	acl	providing,paper
R1266	T2159	T2158	dobj	evidence,providing
R1267	T2160	T2155	punct	", ",paper
R1268	T2161	T2155	prep	by,paper
R1269	T2162	T2161	pobj	Davies,by
R127	T173	T170	prep	in,role
R1270	T2163	T2164	advmod	et,al
R1271	T2164	T2162	advmod	al,Davies
R1272	T2165	T2166	punct	[,26
R1273	T2166	T2155	parataxis	26,paper
R1274	T2167	T2166	punct	],26
R1275	T2168	T2157	punct	", ",is
R1276	T2169	T2170	det	a,study
R1277	T2170	T2157	attr	study,is
R1278	T2171	T2170	amod	biochemical,study
R1279	T2172	T2170	prep	of,study
R128	T174	T175	npadvmod	RAD51,mediated
R1280	T2173	T2174	det	the,interaction
R1281	T2174	T2172	pobj	interaction,of
R1282	T2175	T2174	prep	between,interaction
R1283	T2176	T2177	det	the,RAD51
R1284	T2177	T2175	pobj	RAD51,between
R1285	T2178	T2177	amod	homologous,RAD51
R1286	T2179	T2177	compound	recombinase,RAD51
R1287	T2180	T2177	cc	and,RAD51
R1288	T2181	T2182	det	a,peptide
R1289	T2182	T2177	conj	peptide,RAD51
R129	T175	T177	amod	mediated,repair
R1290	T2183	T2182	acl	consisting,peptide
R1291	T2184	T2183	prep	of,consisting
R1292	T2185	T2184	pobj	one,of
R1293	T2186	T2185	prep	of,one
R1294	T2187	T2188	det	the,repeats
R1295	T2188	T2186	pobj	repeats,of
R1296	T2189	T2188	nummod	eight,repeats
R1297	T2190	T2188	compound	BRC,repeats
R1298	T2191	T2188	prep	from,repeats
R1299	T2192	T2193	amod	human,BRCA2
R13	T51	T52	det	the,role
R130	T176	T175	punct	–,mediated
R1300	T2193	T2191	pobj	BRCA2,from
R1301	T2194	T2157	punct	.,is
R1302	T2196	T2197	nsubjpass	Expression,reported
R1303	T2198	T2196	prep	of,Expression
R1304	T2199	T2200	det	a,repeat
R1305	T2200	T2198	pobj	repeat,of
R1306	T2201	T2200	amod	single,repeat
R1307	T2202	T2200	compound	BRC,repeat
R1308	T2203	T2200	punct	(,repeat
R1309	T2204	T2200	appos	BRC4,repeat
R131	T177	T173	pobj	repair,in
R1310	T2205	T2197	punct	),reported
R1311	T2206	T2197	aux	had,reported
R1312	T2207	T2197	advmod	previously,reported
R1313	T2208	T2197	auxpass	been,reported
R1314	T2209	T2210	aux	to,act
R1315	T2210	T2197	xcomp	act,reported
R1316	T2211	T2210	prep	as,act
R1317	T2212	T2213	det	an,inhibitor
R1318	T2213	T2211	pobj	inhibitor,as
R1319	T2214	T2213	prep	of,inhibitor
R132	T178	T163	punct	.,discuss
R1320	T2215	T2216	compound	DNA,repair
R1321	T2216	T2214	pobj	repair,of
R1322	T2217	T2197	agent	by,reported
R1323	T2218	T2217	pobj	Chen,by
R1324	T2219	T2220	advmod	et,al
R1325	T2220	T2218	advmod	al,Chen
R1326	T2221	T2222	punct	[,27
R1327	T2222	T2197	parataxis	27,reported
R1328	T2223	T2222	punct	],27
R1329	T2224	T2197	punct	.,reported
R1330	T2226	T2227	det	These,investigators
R1331	T2227	T2228	nsubj	investigators,showed
R1332	T2229	T2230	mark	that,enhances
R1333	T2230	T2228	ccomp	enhances,showed
R1334	T2231	T2230	nsubj	expression,enhances
R1335	T2232	T2231	prep	of,expression
R1336	T2233	T2232	pobj	constructs,of
R1337	T2234	T2233	acl	containing,constructs
R1338	T2235	T2236	det	the,repeat
R1339	T2236	T2234	dobj	repeat,containing
R1340	T2237	T2236	compound	BRC4,repeat
R1341	T2238	T2236	prep	in,repeat
R1342	T2239	T2240	nmod	MCF,cells
R1343	T2240	T2238	pobj	cells,in
R1344	T2241	T2239	punct	-,MCF
R1345	T2242	T2239	nummod	7,MCF
R1346	T2243	T2244	det	the,radiosensitivity
R1347	T2244	T2230	dobj	radiosensitivity,enhances
R1348	T2245	T2244	prep	of,radiosensitivity
R1349	T2246	T2245	pobj	cells,of
R135	T227	T228	nsubj	BRCA2,was
R1350	T2247	T2230	cc	and,enhances
R1351	T2248	T2230	conj	blocks,enhances
R1352	T2249	T2250	preconj	both,delay
R1353	T2250	T2248	dobj	delay,blocks
R1354	T2251	T2250	det	the,delay
R1355	T2252	T2253	compound	G2,M
R1356	T2253	T2250	compound	M,delay
R1357	T2254	T2253	punct	/,M
R1358	T2255	T2250	acl	associated,delay
R1359	T2256	T2255	prep	with,associated
R136	T229	T230	det	the,gene
R1360	T2257	T2256	pobj	damage,with
R1361	T2258	T2250	cc	and,delay
R1362	T2259	T2260	det	the,ability
R1363	T2260	T2250	conj	ability,delay
R1364	T2261	T2260	prep	of,ability
R1365	T2262	T2263	det	the,cells
R1366	T2263	T2261	pobj	cells,of
R1367	T2264	T2263	amod	transfected,cells
R1368	T2265	T2266	aux	to,assembly
R1369	T2266	T2260	acl	assembly,ability
R137	T230	T228	attr	gene,was
R1370	T2267	T2268	amod	subnuclear,foci
R1371	T2268	T2266	dobj	foci,assembly
R1372	T2269	T2268	compound	RAD51,foci
R1373	T2270	T2228	punct	.,showed
R1374	T2272	T2273	prep	In,provide
R1375	T2274	T2275	det	the,paper
R1376	T2275	T2272	pobj	paper,In
R1377	T2276	T2275	prep	by,paper
R1378	T2277	T2276	pobj	Davies,by
R1379	T2278	T2279	advmod	et,al
R138	T231	T230	amod	second,gene
R1380	T2279	T2277	advmod	al,Davies
R1381	T2280	T2273	punct	", ",provide
R1382	T2281	T2273	nsubj	data,provide
R1383	T2282	T2273	dobj	evidence,provide
R1384	T2283	T2282	prep	for,evidence
R1385	T2284	T2285	det	a,mechanism
R1386	T2285	T2283	pobj	mechanism,for
R1387	T2286	T2285	amod	plausible,mechanism
R1388	T2287	T2285	prep	of,mechanism
R1389	T2288	T2289	det	the,effect
R139	T232	T233	compound	breast,cancer
R1390	T2289	T2287	pobj	effect,of
R1391	T2290	T2289	amod	dominant,effect
R1392	T2291	T2289	amod	negative,effect
R1393	T2292	T2289	acl	associated,effect
R1394	T2293	T2292	prep	with,associated
R1395	T2294	T2293	pobj	expression,with
R1396	T2295	T2294	prep	of,expression
R1397	T2296	T2297	det	a,repeat
R1398	T2297	T2295	pobj	repeat,of
R1399	T2298	T2297	amod	single,repeat
R14	T52	T50	pobj	role,to
R140	T233	T234	compound	cancer,susceptibility
R1400	T2299	T2297	compound	BRC,repeat
R1401	T2300	T2273	punct	.,provide
R1402	T2302	T2303	compound	DNA,assays
R1403	T2303	T2305	nsubjpass	assays,used
R1404	T2304	T2303	compound	binding,assays
R1405	T2306	T2303	cc	and,assays
R1406	T2307	T2308	compound	electron,microscopy
R1407	T2308	T2309	compound	microscopy,methods
R1408	T2309	T2303	conj	methods,assays
R1409	T2310	T2305	auxpass	were,used
R141	T234	T230	compound	susceptibility,gene
R1410	T2311	T2312	aux	to,show
R1411	T2312	T2305	advcl	show,used
R1412	T2313	T2314	mark	that,interferes
R1413	T2314	T2312	ccomp	interferes,show
R1414	T2315	T2316	det	a,peptide
R1415	T2316	T2314	nsubj	peptide,interferes
R1416	T2317	T2316	compound	BRC3,peptide
R1417	T2318	T2314	prep	with,interferes
R1418	T2319	T2320	det	the,ability
R1419	T2320	T2318	pobj	ability,with
R142	T235	T236	aux	to,discovered
R1420	T2321	T2320	prep	of,ability
R1421	T2322	T2321	pobj	RAD51,of
R1422	T2323	T2324	aux	to,assemble
R1423	T2324	T2320	acl	assemble,ability
R1424	T2325	T2324	prep	into,assemble
R1425	T2326	T2327	amod	oligomeric,filaments
R1426	T2327	T2325	pobj	filaments,into
R1427	T2328	T2324	prep	on,assemble
R1428	T2329	T2328	pobj	DNA,on
R1429	T2330	T2305	punct	.,used
R143	T236	T230	advcl	discovered,gene
R1430	T2332	T2333	det	A,peptide
R1431	T2333	T2335	nsubjpass	peptide,reported
R1432	T2334	T2333	compound	BRC4,peptide
R1433	T2336	T2335	auxpass	was,reported
R1434	T2337	T2335	advmod	also,reported
R1435	T2338	T2339	aux	to,inhibit
R1436	T2339	T2335	xcomp	inhibit,reported
R1437	T2340	T2341	det	the,interaction
R1438	T2341	T2339	dobj	interaction,inhibit
R1439	T2342	T2343	compound	RAD51,DNA
R144	T237	T236	auxpass	be,discovered
R1440	T2343	T2341	compound	DNA,interaction
R1441	T2344	T2343	punct	–,DNA
R1442	T2345	T2335	punct	.,reported
R1443	T2347	T2348	det	These,experiments
R1444	T2348	T2349	nsubj	experiments,raise
R1445	T2350	T2351	det	the,possibility
R1446	T2351	T2349	dobj	possibility,raise
R1447	T2352	T2353	mark	that,act
R1448	T2353	T2351	acl	act,possibility
R1449	T2354	T2355	det	the,protein
R145	T238	T228	punct	", ",was
R1450	T2355	T2353	nsubj	protein,act
R1451	T2356	T2357	amod	full,length
R1452	T2357	T2355	compound	length,protein
R1453	T2358	T2357	punct	-,length
R1454	T2359	T2355	compound	BRCA2,protein
R1455	T2360	T2353	aux	could,act
R1456	T2361	T2353	prep	as,act
R1457	T2362	T2363	det	a,regulator
R1458	T2363	T2361	pobj	regulator,as
R1459	T2364	T2363	amod	negative,regulator
R146	T239	T228	cc	and,was
R1460	T2365	T2363	prep	of,regulator
R1461	T2366	T2367	amod	inappropriate,interaction
R1462	T2367	T2365	pobj	interaction,of
R1463	T2368	T2369	compound	RAD51,DNA
R1464	T2369	T2367	compound	DNA,interaction
R1465	T2370	T2369	punct	–,DNA
R1466	T2371	T2349	punct	.,raise
R1467	T2373	T2374	det	The,experiment
R1468	T2374	T2376	nsubj	experiment,is
R1469	T2375	T2374	amod	final,experiment
R147	T240	T241	auxpass	was,isolated
R1470	T2377	T2374	acl	reported,experiment
R1471	T2378	T2377	agent	by,reported
R1472	T2379	T2378	pobj	Davies,by
R1473	T2380	T2381	advmod	et,al
R1474	T2381	T2379	advmod	al,Davies
R1475	T2382	T2383	punct	[,26
R1476	T2383	T2379	parataxis	26,Davies
R1477	T2384	T2383	punct	],26
R1478	T2385	T2386	advmod	particularly,important
R1479	T2386	T2376	acomp	important,is
R148	T241	T228	conj	isolated,was
R1480	T2387	T2386	prep	with,important
R1481	T2388	T2387	pobj	respect,with
R1482	T2389	T2388	prep	to,respect
R1483	T2390	T2389	pcomp	understanding,to
R1484	T2391	T2392	det	the,s
R1485	T2392	T2390	dobj	s,understanding
R1486	T2393	T2392	nmod	mechanism,s
R1487	T2394	T2392	punct	(,s
R1488	T2395	T2392	punct	),s
R1489	T2396	T2397	prep	through,promotes
R149	T242	T241	prep	through,isolated
R1490	T2397	T2392	relcl	promotes,s
R1491	T2398	T2396	pobj	which,through
R1492	T2399	T2397	nsubj	BRCA2,promotes
R1493	T2400	T2401	npadvmod	RAD51,dependent
R1494	T2401	T2403	amod	dependent,recombination
R1495	T2402	T2401	punct	-,dependent
R1496	T2403	T2397	dobj	recombination,promotes
R1497	T2404	T2376	punct	.,is
R1498	T2406	T2407	det	The,localization
R1499	T2407	T2408	nsubjpass	localization,examined
R15	T53	T52	prep	of,role
R150	T243	T244	amod	positional,cloning
R1500	T2409	T2407	prep	of,localization
R1501	T2410	T2409	pobj	RAD51,of
R1502	T2411	T2408	auxpass	was,examined
R1503	T2412	T2408	prep	in,examined
R1504	T2413	T2414	nmod	CAPAN,cells
R1505	T2414	T2412	pobj	cells,in
R1506	T2415	T2413	punct	-,CAPAN
R1507	T2416	T2413	nummod	1,CAPAN
R1508	T2417	T2414	punct	", ",cells
R1509	T2418	T2419	det	the,line
R151	T244	T242	pobj	cloning,through
R1510	T2419	T2414	appos	line,cells
R1511	T2420	T2419	amod	same,line
R1512	T2421	T2422	amod	human,tumor
R1513	T2422	T2419	compound	tumor,line
R1514	T2423	T2419	acl	examined,line
R1515	T2424	T2423	agent	by,examined
R1516	T2425	T2424	pobj	Moynahan,by
R1517	T2426	T2427	advmod	et,al
R1518	T2427	T2425	advmod	al,Moynahan
R1519	T2428	T2429	punct	[,24
R152	T245	T241	advcl	using,isolated
R1520	T2429	T2425	parataxis	24,Moynahan
R1521	T2430	T2429	punct	],24
R1522	T2431	T2408	punct	.,examined
R1523	T2433	T2434	nsubjpass	It,shown
R1524	T2435	T2434	aux	had,shown
R1525	T2436	T2434	auxpass	been,shown
R1526	T2437	T2434	advmod	previously,shown
R1527	T2438	T2439	mark	that,mislocalized
R1528	T2439	T2434	ccomp	mislocalized,shown
R1529	T2440	T2441	det	the,amount
R153	T246	T245	dobj	data,using
R1530	T2441	T2439	nsubjpass	amount,mislocalized
R1531	T2442	T2441	amod	small,amount
R1532	T2443	T2441	prep	of,amount
R1533	T2444	T2445	amod	truncated,protein
R1534	T2445	T2443	pobj	protein,of
R1535	T2446	T2445	compound	BRCA2,protein
R1536	T2447	T2441	acl	expressed,amount
R1537	T2448	T2447	prep	by,expressed
R1538	T2449	T2450	nmod	CAPAN,cells
R1539	T2450	T2448	pobj	cells,by
R154	T247	T246	prep	from,data
R1540	T2451	T2449	punct	-,CAPAN
R1541	T2452	T2449	nummod	1,CAPAN
R1542	T2453	T2439	auxpass	was,mislocalized
R1543	T2454	T2439	prep	to,mislocalized
R1544	T2455	T2456	det	the,cytoplasm
R1545	T2456	T2454	pobj	cytoplasm,to
R1546	T2457	T2439	punct	", ",mislocalized
R1547	T2458	T2439	advcl	consistent,mislocalized
R1548	T2459	T2458	prep	with,consistent
R1549	T2460	T2461	det	the,fact
R155	T248	T247	pobj	families,from
R1550	T2461	T2459	pobj	fact,with
R1551	T2462	T2463	mark	that,lacks
R1552	T2463	T2461	acl	lacks,fact
R1553	T2464	T2465	det	the,protein
R1554	T2465	T2463	nsubj	protein,lacks
R1555	T2466	T2465	compound	truncation,protein
R1556	T2467	T2468	det	a,signal
R1557	T2468	T2463	dobj	signal,lacks
R1558	T2469	T2468	amod	nuclear,signal
R1559	T2470	T2468	compound	localization,signal
R156	T249	T248	prep	with,families
R1560	T2471	T2472	punct	[,28
R1561	T2472	T2434	parataxis	28,shown
R1562	T2473	T2472	punct	],28
R1563	T2474	T2434	punct	.,shown
R1564	T2476	T2477	nsubj	Davies,showed
R1565	T2478	T2479	advmod	et,al
R1566	T2479	T2476	advmod	al,Davies
R1567	T2480	T2481	nmod	CAPAN,cells
R1568	T2481	T2484	nsubj	cells,be
R1569	T2482	T2480	punct	-,CAPAN
R157	T250	T251	amod	inherited,cancer
R1570	T2483	T2480	nummod	1,CAPAN
R1571	T2484	T2477	ccomp	be,showed
R1572	T2485	T2484	aux	to,be
R1573	T2486	T2484	acomp	defective,be
R1574	T2487	T2484	prep	in,be
R1575	T2488	T2489	amod	nuclear,localization
R1576	T2489	T2487	pobj	localization,in
R1577	T2490	T2489	prep	of,localization
R1578	T2491	T2490	pobj	RAD51,of
R1579	T2492	T2477	punct	", ",showed
R158	T251	T249	pobj	cancer,with
R1580	T2493	T2477	advcl	raising,showed
R1581	T2494	T2495	det	the,possibility
R1582	T2495	T2493	dobj	possibility,raising
R1583	T2496	T2497	mark	that,carried
R1584	T2497	T2495	acl	carried,possibility
R1585	T2498	T2497	nsubjpass	RAD51,carried
R1586	T2499	T2497	auxpass	is,carried
R1587	T2500	T2497	advmod	normally,carried
R1588	T2501	T2497	prep	to,carried
R1589	T2502	T2503	det	the,nucleus
R159	T252	T251	compound	breast,cancer
R1590	T2503	T2501	pobj	nucleus,to
R1591	T2504	T2497	prep	by,carried
R1592	T2505	T2504	pcomp	binding,by
R1593	T2506	T2505	dobj	BRCA2,binding
R1594	T2507	T2477	punct	.,showed
R1595	T2509	T2510	nsubj	It,is
R1596	T2511	T2510	acomp	possible,is
R1597	T2512	T2513	mark	that,be
R1598	T2513	T2510	ccomp	be,is
R1599	T2514	T2513	nsubj	RAD51,be
R16	T54	T55	det	the,gene
R160	T253	T254	punct	[,4
R1600	T2515	T2513	aux	may,be
R1601	T2516	T2513	advmod	alternatively,be
R1602	T2517	T2513	acomp	capable,be
R1603	T2518	T2517	prep	of,capable
R1604	T2519	T2520	npadvmod	BRCA2,independent
R1605	T2520	T2522	amod	independent,transport
R1606	T2521	T2520	punct	-,independent
R1607	T2522	T2518	pobj	transport,of
R1608	T2523	T2510	punct	", ",is
R1609	T2524	T2510	cc	but,is
R161	T254	T241	parataxis	4,isolated
R1610	T2525	T2526	nsubj	mislocalization,sequester
R1611	T2526	T2510	conj	sequester,is
R1612	T2527	T2525	prep	of,mislocalization
R1613	T2528	T2527	pobj	BRCA2,of
R1614	T2529	T2525	prep	to,mislocalization
R1615	T2530	T2531	det	the,cytoplasm
R1616	T2531	T2529	pobj	cytoplasm,to
R1617	T2532	T2526	aux	may,sequester
R1618	T2533	T2526	dobj	RAD51,sequester
R1619	T2534	T2526	cc	and,sequester
R162	T255	T254	punct	],4
R1620	T2535	T2526	conj	block,sequester
R1621	T2536	T2537	poss	its,mode
R1622	T2537	T2535	dobj	mode,block
R1623	T2538	T2537	amod	normal,mode
R1624	T2539	T2537	prep	of,mode
R1625	T2540	T2539	pobj	transport,of
R1626	T2541	T2526	punct	.,sequester
R1627	T2543	T2544	nsubj	Support,requires
R1628	T2545	T2543	prep	for,Support
R1629	T2546	T2547	det	the,hypothesis
R163	T256	T228	punct	.,was
R1630	T2547	T2545	pobj	hypothesis,for
R1631	T2548	T2549	mark	that,is
R1632	T2549	T2547	acl	is,hypothesis
R1633	T2550	T2551	det	the,mislocalization
R1634	T2551	T2549	nsubj	mislocalization,is
R1635	T2552	T2551	amod	observed,mislocalization
R1636	T2553	T2551	prep	of,mislocalization
R1637	T2554	T2553	pobj	RAD51,of
R1638	T2555	T2556	det	an,consequence
R1639	T2556	T2549	attr	consequence,is
R164	T258	T259	nsubj	Cells,are
R1640	T2557	T2556	amod	indirect,consequence
R1641	T2558	T2556	prep	of,consequence
R1642	T2559	T2560	det	the,defect
R1643	T2560	T2558	pobj	defect,of
R1644	T2561	T2560	compound	BRCA2,defect
R1645	T2562	T2544	dobj	reintroduction,requires
R1646	T2563	T2562	prep	of,reintroduction
R1647	T2564	T2565	amod	functional,BRCA2
R1648	T2565	T2563	pobj	BRCA2,of
R1649	T2566	T2562	prep	into,reintroduction
R165	T260	T258	prep	with,Cells
R1650	T2567	T2566	pobj	CAPAN,into
R1651	T2568	T2567	punct	-,CAPAN
R1652	T2569	T2567	nummod	1,CAPAN
R1653	T2570	T2544	punct	.,requires
R1654	T2572	T2573	prep	Given,seems
R1655	T2574	T2575	det	the,nature
R1656	T2575	T2572	pobj	nature,Given
R1657	T2576	T2575	amod	elaborate,nature
R1658	T2577	T2575	prep	of,nature
R1659	T2578	T2579	det	the,interaction
R166	T261	T262	amod	mutant,protein
R1660	T2579	T2577	pobj	interaction,of
R1661	T2580	T2581	compound	RAD51,BRCA2
R1662	T2581	T2579	compound	BRCA2,interaction
R1663	T2582	T2581	punct	–,BRCA2
R1664	T2583	T2573	punct	", ",seems
R1665	T2584	T2573	nsubj	it,seems
R1666	T2585	T2586	advmod	somewhat,unlikely
R1667	T2586	T2573	oprd	unlikely,seems
R1668	T2587	T2588	mark	that,is
R1669	T2588	T2573	ccomp	is,seems
R167	T262	T260	pobj	protein,with
R1670	T2589	T2590	det	the,role
R1671	T2590	T2588	nsubj	role,is
R1672	T2591	T2590	amod	only,role
R1673	T2592	T2590	prep	of,role
R1674	T2593	T2592	pobj	BRCA2,of
R1675	T2594	T2590	prep	in,role
R1676	T2595	T2594	pcomp	promoting,in
R1677	T2596	T2595	dobj	recombination,promoting
R1678	T2597	T2598	aux	to,serve
R1679	T2598	T2588	xcomp	serve,is
R168	T263	T262	compound	BRCA2,protein
R1680	T2599	T2598	prep	as,serve
R1681	T2600	T2601	det	a,transporter
R1682	T2601	T2599	pobj	transporter,as
R1683	T2602	T2601	amod	specific,transporter
R1684	T2603	T2601	amod	nuclear,transporter
R1685	T2604	T2601	prep	of,transporter
R1686	T2605	T2604	pobj	RAD51,of
R1687	T2606	T2573	punct	.,seems
R1688	T2608	T2609	det	An,alternative
R1689	T2609	T2611	nsubj	alternative,is
R169	T264	T259	punct	", ",are
R1690	T2610	T2609	amod	attractive,alternative
R1691	T2612	T2609	punct	", ",alternative
R1692	T2613	T2609	acl	favored,alternative
R1693	T2614	T2613	agent	by,favored
R1694	T2615	T2614	pobj	Davies,by
R1695	T2616	T2617	advmod	et,al
R1696	T2617	T2615	advmod	al,Davies
R1697	T2618	T2619	punct	[,26
R1698	T2619	T2615	parataxis	26,Davies
R1699	T2620	T2619	punct	],26
R17	T55	T53	pobj	gene,of
R170	T265	T259	prep	like,are
R1700	T2621	T2611	punct	", ",is
R1701	T2622	T2623	mark	that,required
R1702	T2623	T2611	ccomp	required,is
R1703	T2624	T2625	det	the,association
R1704	T2625	T2623	nsubjpass	association,required
R1705	T2626	T2625	prep	of,association
R1706	T2627	T2628	compound	RAD51,BRCA2
R1707	T2628	T2626	pobj	BRCA2,of
R1708	T2629	T2628	punct	–,BRCA2
R1709	T2630	T2623	auxpass	is,required
R171	T266	T267	amod	many,cells
R1710	T2631	T2632	aux	to,maintain
R1711	T2632	T2623	advcl	maintain,required
R1712	T2633	T2632	dobj	RAD51,maintain
R1713	T2634	T2632	prep	in,maintain
R1714	T2635	T2636	det	a,form
R1715	T2636	T2634	pobj	form,in
R1716	T2637	T2638	dep	that,is
R1717	T2638	T2636	relcl	is,form
R1718	T2639	T2638	prep	in,is
R1719	T2640	T2641	det	a,state
R172	T267	T265	pobj	cells,like
R1720	T2641	T2639	pobj	state,in
R1721	T2642	T2641	prep	of,state
R1722	T2643	T2642	punct	',of
R1723	T2644	T2642	pobj	readiness,of
R1724	T2645	T2611	punct	',is
R1725	T2646	T2647	punct	(,2a
R1726	T2647	T2611	parataxis	2a,is
R1727	T2648	T2647	compound	Fig.,2a
R1728	T2649	T2647	punct	),2a
R1729	T2650	T2611	punct	.,is
R173	T268	T267	compound	cancer,cells
R1730	T2652	T2653	prep	In,exist
R1731	T2654	T2655	det	the,absence
R1732	T2655	T2652	pobj	absence,In
R1733	T2656	T2655	prep	of,absence
R1734	T2657	T2658	det	this,interaction
R1735	T2658	T2656	pobj	interaction,of
R1736	T2659	T2658	prep	with,interaction
R1737	T2660	T2659	pobj	BRCA2,with
R1738	T2661	T2653	punct	", ",exist
R1739	T2662	T2653	nsubj	RAD51,exist
R174	T269	T259	punct	", ",are
R1740	T2663	T2653	aux	might,exist
R1741	T2664	T2653	prep	in,exist
R1742	T2665	T2666	det	a,form
R1743	T2666	T2664	pobj	form,in
R1744	T2667	T2668	dep	that,is
R1745	T2668	T2666	relcl	is,form
R1746	T2669	T2668	neg	not,is
R1747	T2670	T2668	acomp	capable,is
R1748	T2671	T2670	prep	of,capable
R1749	T2672	T2673	auxpass	being,recruited
R175	T270	T271	advmod	genetically,unstable
R1750	T2673	T2671	pcomp	recruited,of
R1751	T2674	T2673	prep	into,recruited
R1752	T2675	T2676	det	a,complex
R1753	T2676	T2674	pobj	complex,into
R1754	T2677	T2676	amod	functional,complex
R1755	T2678	T2676	compound	repair,complex
R1756	T2679	T2680	advmod	when,occurs
R1757	T2680	T2673	advcl	occurs,recruited
R1758	T2681	T2680	nsubj	damage,occurs
R1759	T2682	T2653	punct	.,exist
R176	T271	T259	acomp	unstable,are
R1760	T2684	T2685	prep	For,indicated
R1761	T2686	T2684	pobj	example,For
R1762	T2687	T2685	punct	", ",indicated
R1763	T2688	T2689	amod	previous,studies
R1764	T2689	T2685	nsubj	studies,indicated
R1765	T2690	T2691	mark	that,shows
R1766	T2691	T2685	ccomp	shows,indicated
R1767	T2692	T2691	nsubj	RAD51,shows
R1768	T2693	T2694	amod	little,preference
R1769	T2694	T2691	dobj	preference,shows
R177	T272	T259	cc	and,are
R1770	T2695	T2694	compound	binding,preference
R1771	T2696	T2694	prep	for,preference
R1772	T2697	T2698	amod	single,strand
R1773	T2698	T2700	compound	strand,DNA
R1774	T2699	T2698	punct	-,strand
R1775	T2700	T2696	pobj	DNA,for
R1776	T2701	T2700	punct	(,DNA
R1777	T2702	T2700	appos	ssDNA,DNA
R1778	T2703	T2694	punct	),preference
R1779	T2704	T2694	prep	over,preference
R178	T273	T259	conj	accumulate,are
R1780	T2705	T2706	amod	double,strand
R1781	T2706	T2708	compound	strand,DNA
R1782	T2707	T2706	punct	-,strand
R1783	T2708	T2704	pobj	DNA,over
R1784	T2709	T2708	punct	(,DNA
R1785	T2710	T2708	appos	dsDNA,DNA
R1786	T2711	T2685	punct	),indicated
R1787	T2712	T2713	punct	[,29
R1788	T2713	T2685	parataxis	29,indicated
R1789	T2714	T2713	nummod	3,29
R179	T274	T275	amod	gross,rearrangements
R1790	T2715	T2713	punct	",",29
R1791	T2716	T2713	punct	],29
R1792	T2717	T2685	punct	.,indicated
R1793	T2719	T2720	det	This,result
R1794	T2720	T2721	nsubj	result,mean
R1795	T2722	T2721	aux	could,mean
R1796	T2723	T2724	mark	that,required
R1797	T2724	T2721	ccomp	required,mean
R1798	T2725	T2726	amod	regulatory,factors
R1799	T2726	T2724	nsubjpass	factors,required
R18	T56	T57	compound	breast,cancer
R180	T275	T273	dobj	rearrangements,accumulate
R1800	T2727	T2726	punct	", ",factors
R1801	T2728	T2729	amod	such,as
R1802	T2729	T2726	prep	as,factors
R1803	T2730	T2729	pobj	BRCA2,as
R1804	T2731	T2724	punct	", ",required
R1805	T2732	T2724	auxpass	are,required
R1806	T2733	T2734	aux	to,suppress
R1807	T2734	T2724	advcl	suppress,required
R1808	T2735	T2736	compound	RAD51,dsDNA
R1809	T2736	T2738	compound	dsDNA,interactions
R181	T276	T275	amod	chromosomal,rearrangements
R1810	T2737	T2736	punct	–,dsDNA
R1811	T2738	T2734	dobj	interactions,suppress
R1812	T2740	T2724	advcl	prevent,required
R1813	T2741	T2740	dobj	sequestration,prevent
R1814	T2742	T2741	prep	of,sequestration
R1815	T2743	T2742	pobj	RAD51,of
R1816	T2744	T2741	prep	in,sequestration
R1817	T2745	T2746	det	a,form
R1818	T2746	T2744	pobj	form,in
R1819	T2747	T2746	amod	nonfunctional,form
R182	T277	T278	punct	[,6
R1820	T2748	T2721	punct	.,mean
R1821	T2750	T2751	advmod	Alternatively,in
R1822	T2751	T2754	prep	in,be
R1823	T2752	T2751	punct	", ",in
R1824	T2739	T2740	aux	to,prevent
R1825	T2755	T2751	pobj	addition,in
R1826	T2756	T2754	punct	", ",be
R1827	T2753	T2751	cc	or,in
R1828	T2758	T2757	prep	of,suppression
R1829	T2759	T2760	compound	RAD51,ssDNA
R183	T278	T273	parataxis	6,accumulate
R1830	T2757	T2754	nsubj	suppression,be
R1831	T2761	T2760	punct	–,ssDNA
R1832	T2762	T2758	pobj	interaction,of
R1833	T2760	T2762	compound	ssDNA,interaction
R1834	T2764	T2754	acomp	important,be
R1835	T2765	T2754	prep	for,be
R1836	T2763	T2754	aux	might,be
R1837	T2767	T2766	dobj	RAD51,preventing
R1838	T2768	T2766	prep	from,preventing
R1839	T2766	T2765	pcomp	preventing,for
R184	T279	T278	nummod	5,6
R1840	T2770	T2769	prep	to,binding
R1841	T2771	T2772	det	the,regions
R1842	T2769	T2768	pcomp	binding,from
R1843	T2773	T2772	prep	of,regions
R1844	T2774	T2773	pobj	ssDNA,of
R1845	T2772	T2770	pobj	regions,to
R1846	T2776	T2772	relcl	form,regions
R1847	T2777	T2776	prep	during,form
R1848	T2775	T2776	dep	that,form
R1849	T2779	T2777	pobj	replication,during
R185	T280	T278	punct	",",6
R1850	T2780	T2779	compound	DNA,replication
R1851	T2781	T2754	punct	.,be
R1852	T2778	T2779	amod	normal,replication
R1853	T2783	T2784	nsubj	BRCA2,promote
R1854	T2785	T2784	aux	may,promote
R1855	T2786	T2784	advmod	thus,promote
R1856	T2788	T2784	dobj	assembly,promote
R1857	T2789	T2788	prep	into,assembly
R1858	T2787	T2788	compound	RAD51,assembly
R1859	T2791	T2789	pobj	complexes,into
R186	T281	T278	punct	],6
R1860	T2792	T2791	compound	repair,complexes
R1861	T2790	T2791	amod	recombinational,complexes
R1862	T2794	T2795	det	a,mechanism
R1863	T2795	T2793	pobj	mechanism,via
R1864	T2796	T2795	amod	negative,mechanism
R1865	T2793	T2784	prep	via,promote
R1866	T2798	T2793	punct	(,via
R1867	T2799	T2800	advmod	i.e.,by
R1868	T2797	T2795	amod	regulatory,mechanism
R1869	T2801	T2800	pcomp	blocking,by
R187	T282	T259	punct	.,are
R1870	T2802	T2803	compound	RAD51–BRCA2,interaction
R1871	T2800	T2793	prep	by,via
R1872	T2804	T2805	mark	until,occurred
R1873	T2805	T2801	advcl	occurred,blocking
R1874	T2803	T2801	dobj	interaction,blocking
R1875	T2807	T2805	aux	has,occurred
R1876	T2808	T2805	cc	and,occurred
R1877	T2806	T2805	nsubj	damage,occurred
R1878	T2810	T2805	conj	are,occurred
R1879	T2811	T2809	acl	required,factors
R188	T284	T285	det	The,sequence
R1880	T2809	T2810	nsubj	factors,are
R1881	T2813	T2812	punct	',for
R1882	T2812	T2811	prep	for,required
R1883	T2815	T2812	pobj	assembly,for
R1884	T2814	T2815	amod	productive,assembly
R1885	T2817	T2815	prep	of,assembly
R1886	T2816	T2815	punct	',assembly
R1887	T2819	T2815	prep	at,assembly
R1888	T2818	T2817	pobj	RAD51,of
R1889	T2821	T2819	pobj	sites,at
R189	T285	T286	nsubj	sequence,offers
R1890	T2822	T2810	prep	in,are
R1891	T2820	T2821	amod	damaged,sites
R1892	T2824	T2784	punct	),promote
R1893	T2825	T2784	punct	.,promote
R1894	T2823	T2822	pobj	place,in
R1895	T2827	T2828	mark	As,mentioned
R1896	T2828	T2829	advcl	mentioned,reflect
R1897	T2830	T2828	advmod	previously,mentioned
R1898	T2831	T2829	punct	", ",reflect
R1899	T2833	T2829	nsubj	ability,reflect
R19	T57	T58	compound	cancer,susceptibility
R190	T287	T285	prep	of,sequence
R1900	T2832	T2833	det	the,ability
R1901	T2835	T2836	det	a,peptide
R1902	T2836	T2834	pobj	peptide,of
R1903	T2834	T2833	prep	of,ability
R1904	T2838	T2839	aux	to,repress
R1905	T2839	T2833	acl	repress,ability
R1906	T2837	T2836	compound	BRC,peptide
R1907	T2841	T2839	dobj	interaction,repress
R1908	T2842	T2841	compound	RAD51,interaction
R1909	T2840	T2841	det	the,interaction
R191	T288	T289	det	this,protein
R1910	T2844	T2841	compound	BRCA2,interaction
R1911	T2843	T2841	punct	–,interaction
R1912	T2846	T2847	det	a,role
R1913	T2847	T2829	dobj	role,reflect
R1914	T2845	T2829	aux	could,reflect
R1915	T2849	T2850	amod	full,length
R1916	T2850	T2852	compound	length,protein
R1917	T2851	T2850	punct	-,length
R1918	T2848	T2847	prep	of,role
R1919	T2853	T2847	prep	in,role
R192	T289	T287	pobj	protein,of
R1920	T2852	T2848	pobj	protein,of
R1921	T2855	T2856	amod	unwanted,interactions
R1922	T2854	T2853	pcomp	suppressing,in
R1923	T2857	T2858	compound	RAD51,BRCA2
R1924	T2858	T2856	compound	BRCA2,interactions
R1925	T2856	T2854	dobj	interactions,suppressing
R1926	T2860	T2829	punct	.,reflect
R1927	T2862	T2863	advmod	Yet,role
R1928	T2859	T2858	punct	–,BRCA2
R1929	T2864	T2863	det	another,role
R193	T290	T289	amod	large,protein
R1930	T2867	T2863	prep	for,role
R1931	T2863	T2866	nsubj	role,is
R1932	T2865	T2863	amod	potential,role
R1933	T2869	T2863	prep	in,role
R1934	T2870	T2869	pcomp	promoting,in
R1935	T2868	T2867	pobj	BRCA2,for
R1936	T2872	T2874	amod	dependent,repair
R1937	T2873	T2872	punct	-,dependent
R1938	T2871	T2872	npadvmod	RAD51,dependent
R1939	T2875	T2874	amod	recombinational,repair
R194	T291	T285	punct	(,sequence
R1940	T2876	T2877	det	a,role
R1941	T2874	T2870	dobj	repair,promoting
R1942	T2878	T2877	amod	positive,role
R1943	T2877	T2866	attr	role,is
R1944	T2880	T2879	pobj	assembly,in
R1945	T2881	T2880	prep	of,assembly
R1946	T2879	T2877	prep	in,role
R1947	T2883	T2880	prep	at,assembly
R1948	T2884	T2885	amod	damaged,sites
R1949	T2882	T2881	pobj	RAD51,of
R195	T292	T293	nummod	3418,acids
R1950	T2886	T2887	punct	(,2b
R1951	T2885	T2883	pobj	sites,at
R1952	T2888	T2887	compound	Fig.,2b
R1953	T2889	T2887	punct	),2b
R1954	T2887	T2866	parataxis	2b,is
R1955	T2892	T2893	amod	Several,proteins
R1956	T2890	T2866	punct	.,is
R1957	T2895	T2896	dep	that,interact
R1958	T2893	T2894	nsubjpass	proteins,thought
R1959	T2897	T2896	prep	with,interact
R196	T293	T285	appos	acids,sequence
R1960	T2898	T2897	pobj	RAD51,with
R1961	T2896	T2893	relcl	interact,proteins
R1962	T2900	T2901	aux	to,mediate
R1963	T2901	T2894	xcomp	mediate,thought
R1964	T2899	T2894	auxpass	are,thought
R1965	T2903	T2901	punct	',mediate
R1966	T2904	T2901	dobj	assembly,mediate
R1967	T2902	T2901	punct	',mediate
R1968	T2906	T2905	pobj	RAD51,of
R1969	T2907	T2901	prep	at,mediate
R197	T294	T293	compound	amino,acids
R1970	T2905	T2904	prep	of,assembly
R1971	T2909	T2908	prep	of,sites
R1972	T2910	T2911	compound	DNA,damage
R1973	T2908	T2907	pobj	sites,at
R1974	T2912	T2913	punct	(,reviewed
R1975	T2913	T2894	parataxis	reviewed,thought
R1976	T2911	T2909	pobj	damage,of
R1977	T2914	T2913	prep	in,reviewed
R1978	T2916	T2914	pobj	30,in
R1979	T2917	T2913	punct	],reviewed
R198	T295	T286	punct	),offers
R1980	T2915	T2914	punct	[,in
R1981	T2919	T2894	punct	.,thought
R1982	T2918	T2913	punct	),reviewed
R1983	T2921	T2922	compound	RAD51,paralogs
R1984	T2924	T2922	prep	in,paralogs
R1985	T2922	T2923	nsubj	paralogs,function
R1986	T2926	T2922	punct	", ",paralogs
R1987	T2925	T2924	amod	particular,in
R1988	T2928	T2927	pobj	XRCC2,including
R1989	T2929	T2928	punct	", ",XRCC2
R199	T296	T297	advmod	very,clue
R1990	T2927	T2922	prep	including,paralogs
R1991	T2931	T2930	punct	", ",XRCC3
R1992	T2932	T2930	conj	RAD51B,XRCC3
R1993	T2930	T2928	conj	XRCC3,XRCC2
R1994	T2934	T2932	conj	RAD51C,RAD51B
R1995	T2935	T2934	cc	and,RAD51C
R1996	T2933	T2932	punct	", ",RAD51B
R1997	T2937	T2923	punct	", ",function
R1998	T2938	T2923	aux	may,function
R1999	T2936	T2934	conj	RAD51D,RAD51C
R20	T58	T55	compound	susceptibility,gene
R200	T297	T286	dobj	clue,offers
R2000	T2940	T2941	det	a,complex
R2001	T2941	T2939	pobj	complex,as
R2002	T2939	T2923	prep	as,function
R2003	T2943	T2941	relcl	promotes,complex
R2004	T2944	T2943	advmod	actively,promotes
R2005	T2942	T2943	dep	that,promotes
R2006	T2946	T2943	dobj	assembly,promotes
R2007	T2947	T2948	punct	[,31
R2008	T2945	T2946	compound	RAD51,assembly
R2009	T2949	T2948	punct	],31
R201	T298	T297	amod	little,clue
R2010	T2950	T2923	punct	.,function
R2011	T2948	T2923	parataxis	31,function
R2012	T2952	T2953	nsubj	BRCA2,cooperate
R2013	T2954	T2953	aux	could,cooperate
R2014	T2956	T2957	compound	RAD51,paralogs
R2015	T2957	T2955	pobj	paralogs,with
R2016	T2955	T2953	prep	with,cooperate
R2017	T2959	T2958	pcomp	promoting,in
R2018	T2960	T2961	compound	RAD51,assembly
R2019	T2958	T2953	prep	in,cooperate
R202	T299	T297	prep	to,clue
R2020	T2962	T2953	punct	", ",cooperate
R2021	T2963	T2953	cc	or,cooperate
R2022	T2961	T2959	dobj	assembly,promoting
R2023	T2965	T2953	conj	provide,cooperate
R2024	T2966	T2967	det	an,pathway
R2025	T2964	T2965	advmod	even,provide
R2026	T2968	T2967	amod	alternative,pathway
R2027	T2969	T2967	compound	assembly,pathway
R2028	T2967	T2965	dobj	pathway,provide
R2029	T2972	T2973	det	The,observation
R203	T300	T301	poss	its,function
R2030	T2970	T2953	punct	.,cooperate
R2031	T2975	T2976	mark	that,localizes
R2032	T2976	T2973	acl	localizes,observation
R2033	T2973	T2974	nsubj	observation,seems
R2034	T2978	T2976	prep	to,localizes
R2035	T2979	T2980	npadvmod	damage,induced
R2036	T2977	T2976	nsubj	BRCA2,localizes
R2037	T2981	T2980	punct	-,induced
R2038	T2980	T2982	amod	induced,foci
R2039	T2983	T2982	amod	subnuclear,foci
R204	T301	T299	pobj	function,to
R2040	T2982	T2978	pobj	foci,to
R2041	T2985	T2984	pobj	RAD51,with
R2042	T2986	T2974	oprd	consistent,seems
R2043	T2984	T2976	prep	with,localizes
R2044	T2988	T2989	det	the,possibility
R2045	T2987	T2986	prep	with,consistent
R2046	T2990	T2991	mark	that,plays
R2047	T2989	T2987	pobj	possibility,with
R2048	T2992	T2991	nsubj	BRCA2,plays
R2049	T2991	T2989	acl	plays,possibility
R205	T302	T286	punct	", ",offers
R2050	T2994	T2991	dobj	role,plays
R2051	T2995	T2994	amod	positive,role
R2052	T2993	T2994	det	a,role
R2053	T2997	T2996	pobj	assembly,in
R2054	T2996	T2991	prep	in,plays
R2055	T2999	T3000	det	the,complex
R2056	T3000	T2998	pobj	complex,of
R2057	T2998	T2997	prep	of,assembly
R2058	T3002	T3003	punct	[,11
R2059	T3001	T3000	compound	recombination,complex
R206	T303	T304	mark	although,are
R2060	T3004	T3003	punct	],11
R2061	T3003	T2974	parataxis	11,seems
R2062	T3007	T3008	advmod	Finally,is
R2063	T3005	T2974	punct	.,seems
R2064	T3009	T3008	punct	", ",is
R2065	T3011	T3008	acomp	important,is
R2066	T3012	T3013	aux	to,note
R2067	T3010	T3008	nsubj	it,is
R2068	T3014	T3015	mark	that,are
R2069	T3015	T3013	ccomp	are,note
R207	T304	T286	advcl	are,offers
R2070	T3013	T3008	xcomp	note,is
R2071	T3017	T3015	nsubj	models,are
R2072	T3018	T3017	nummod	two,models
R2073	T3016	T3017	det	the,models
R2074	T3020	T3021	det	the,function
R2075	T3021	T3019	pobj	function,for
R2076	T3022	T3021	prep	of,function
R2077	T3019	T3017	prep	for,models
R2078	T3024	T3022	pobj	interaction,of
R2079	T3025	T3026	compound	BRCA2,RAD51
R208	T305	T304	expl	there,are
R2080	T3023	T3024	det	the,interaction
R2081	T3026	T3024	compound	RAD51,interaction
R2082	T3028	T3017	acl	shown,models
R2083	T3029	T3028	prep	in,shown
R2084	T3027	T3026	punct	–,RAD51
R2085	T3031	T3030	nummod	2,Figure
R2086	T3030	T3029	pobj	Figure,in
R2087	T3033	T3034	advmod	mutually,exclusive
R2088	T3034	T3015	acomp	exclusive,are
R2089	T3032	T3015	neg	not,are
R209	T306	T307	nummod	eight,segments
R2090	T3039	T3038	aux	could,prevent
R2091	T3035	T3008	punct	.,is
R2092	T3041	T3038	dobj	assembly,prevent
R2093	T3037	T3038	nsubj	BRCA2,prevent
R2094	T3042	T3041	compound	RAD51,assembly
R2095	T3040	T3041	amod	inappropriate,assembly
R2096	T3044	T3045	det	the,absence
R2097	T3043	T3038	prep	in,prevent
R2098	T3046	T3045	prep	of,absence
R2099	T3045	T3043	pobj	absence,in
R21	T59	T55	appos	BRCA2,gene
R210	T307	T304	attr	segments,are
R2100	T3048	T3038	cc	and,prevent
R2101	T3049	T3038	conj	promote,prevent
R2102	T3047	T3046	pobj	damage,of
R2103	T3051	T3049	dobj	assembly,promote
R2104	T3052	T3051	compound	RAD51,assembly
R2105	T3050	T3051	amod	functional,assembly
R2106	T3054	T3055	compound	DNA,lesions
R2107	T3055	T3053	pobj	lesions,at
R2108	T3053	T3051	prep	at,assembly
R2109	T3057	T3058	det	the,response
R211	T308	T307	amod	repeated,segments
R2110	T3056	T3049	prep	during,promote
R2111	T3059	T3058	compound	damage,response
R2112	T3060	T3038	punct	.,prevent
R2113	T3058	T3056	pobj	response,during
R2114	T3062	T3063	amod	Further,experiments
R2115	T3065	T3064	auxpass	are,required
R2116	T3066	T3064	advmod	clearly,required
R2117	T3063	T3064	nsubjpass	experiments,required
R2118	T3068	T3064	advcl	clarify,required
R2119	T3069	T3070	det	the,interactions
R212	T309	T310	punct	(,termed
R2120	T3067	T3068	aux	to,clarify
R2121	T3071	T3070	amod	functional,interactions
R2122	T3070	T3068	dobj	interactions,clarify
R2123	T3073	T3072	pobj	BRCA2,between
R2124	T3074	T3073	cc	and,BRCA2
R2125	T3072	T3070	prep	between,interactions
R2126	T3076	T3068	prep	during,clarify
R2127	T3077	T3078	amod	recombinational,repair
R2128	T3075	T3073	conj	RAD51,BRCA2
R2129	T3079	T3064	punct	.,required
R213	T310	T307	parataxis	termed,segments
R2130	T3078	T3076	pobj	repair,during
R2131	T3083	T3082	aux	should,be
R2132	T3084	T3085	advmod	relatively,straightforward
R2133	T3085	T3082	acomp	straightforward,be
R2134	T3081	T3082	nsubj	It,be
R2135	T3087	T3082	xcomp	determine,be
R2136	T3089	T3087	ccomp	limited,determine
R2137	T3086	T3087	aux	to,determine
R2138	T3090	T3091	det	the,role
R2139	T3088	T3089	mark	whether,limited
R214	T311	T312	compound	BRC,repeats
R2140	T3092	T3091	prep	of,role
R2141	T3091	T3089	nsubjpass	role,limited
R2142	T3094	T3089	auxpass	is,limited
R2143	T3095	T3089	prep	to,limited
R2144	T3093	T3092	pobj	BRCA2,of
R2145	T3097	T3096	dobj	RAD51,transporting
R2146	T3096	T3095	pcomp	transporting,to
R2147	T3099	T3100	det	the,nucleus
R2148	T3100	T3098	pobj	nucleus,to
R2149	T3098	T3096	prep	to,transporting
R215	T312	T310	oprd	repeats,termed
R2150	T3103	T3104	mark	If,were
R2151	T3101	T3082	punct	.,be
R2152	T3105	T3104	nsubj	this,were
R2153	T3108	T3104	attr	case,were
R2154	T3104	T3106	advcl	were,suppress
R2155	T3109	T3106	punct	", ",suppress
R2156	T3107	T3108	det	the,case
R2157	T3111	T3110	prep	of,addition
R2158	T3112	T3113	det	a,signal
R2159	T3113	T3111	pobj	signal,of
R216	T313	T310	punct	),termed
R2160	T3110	T3106	nsubj	addition,suppress
R2161	T3115	T3113	compound	localization,signal
R2162	T3116	T3110	prep	to,addition
R2163	T3114	T3113	amod	nuclear,signal
R2164	T3118	T3106	aux	should,suppress
R2165	T3119	T3120	det	the,phenotypes
R2166	T3117	T3116	pobj	RAD51,to
R2167	T3121	T3122	npadvmod	RAD51,mediated
R2168	T3122	T3120	amod	mediated,phenotypes
R2169	T3120	T3106	dobj	phenotypes,suppress
R217	T314	T307	prep	in,segments
R2170	T3124	T3120	acl	associated,phenotypes
R2171	T3125	T3124	prep	with,associated
R2172	T3123	T3122	punct	-,mediated
R2173	T3127	T3125	pobj	mutation,with
R2174	T3128	T3127	compound	BRCA2,mutation
R2175	T3126	T3127	det	a,mutation
R2176	T3133	T3132	punct	", ",needed
R2177	T3129	T3106	punct	.,suppress
R2178	T3131	T3132	advmod	However,needed
R2179	T3135	T3132	nsubjpass	experiments,needed
R218	T315	T316	det	the,middle
R2180	T3136	T3132	auxpass	are,needed
R2181	T3134	T3135	amod	biochemical,experiments
R2182	T3138	T3132	advcl	determine,needed
R2183	T3137	T3138	aux	to,determine
R2184	T3140	T3138	ccomp	blocks,determine
R2185	T3141	T3142	amod	full,length
R2186	T3139	T3140	mark	whether,blocks
R2187	T3143	T3142	punct	-,length
R2188	T3144	T3140	nsubj	BRCA2,blocks
R2189	T3142	T3144	compound	length,BRCA2
R219	T316	T314	pobj	middle,in
R2190	T3146	T3140	conj	promotes,blocks
R2191	T3147	T3148	compound	RAD51,BRCA2
R2192	T3145	T3140	cc	or,blocks
R2193	T3149	T3148	punct	–,BRCA2
R2194	T3148	T3150	compound	BRCA2,interaction
R2195	T3151	T3132	punct	.,needed
R2196	T3154	T3155	nsubj	experiments,reveal
R2197	T3150	T3146	dobj	interaction,promotes
R2198	T3153	T3154	amod	Such,experiments
R2199	T3156	T3155	aux	could,reveal
R22	T60	T52	prep	in,role
R220	T317	T316	prep	of,middle
R2200	T3158	T3159	amod	additional,roles
R2201	T3159	T3155	dobj	roles,reveal
R2202	T3157	T3155	advmod	also,reveal
R2203	T3161	T3160	pobj	BRCA2,for
R2204	T3162	T3159	prep	in,roles
R2205	T3160	T3159	prep	for,roles
R2206	T3164	T3162	pobj	repair,in
R2207	T3163	T3164	amod	recombinational,repair
R2208	T3169	T3168	punct	", ",promise
R2209	T3165	T3155	punct	.,reveal
R221	T318	T319	det	the,protein
R2210	T3167	T3168	advmod	However,promise
R2211	T3171	T3168	nsubj	obstacles,promise
R2212	T3172	T3171	amod	technical,obstacles
R2213	T3170	T3171	amod	several,obstacles
R2214	T3174	T3171	prep	including,obstacles
R2215	T3175	T3176	det	the,size
R2216	T3173	T3171	punct	", ",obstacles
R2217	T3177	T3176	amod	large,size
R2218	T3178	T3176	prep	of,size
R2219	T3176	T3174	pobj	size,including
R222	T319	T317	pobj	protein,of
R2220	T3180	T3168	punct	", ",promise
R2221	T3181	T3182	aux	to,make
R2222	T3182	T3168	xcomp	make,promise
R2223	T3179	T3178	pobj	BRCA2,of
R2224	T3184	T3185	nsubj	characterization,difficult
R2225	T3185	T3182	ccomp	difficult,make
R2226	T3183	T3184	amod	biochemical,characterization
R2227	T3187	T3188	compound	BRCA2,function
R2228	T3186	T3184	prep	of,characterization
R2229	T3189	T3168	punct	.,promise
R223	T320	T321	dep	that,are
R2231	T3188	T3186	pobj	function,of
R224	T321	T307	relcl	are,segments
R225	T322	T323	advmod	highly,conserved
R226	T323	T321	acomp	conserved,are
R227	T324	T321	prep	among,are
R228	T325	T326	amod	mammalian,orthologs
R229	T326	T324	pobj	orthologs,among
R23	T61	T62	compound	DNA,repair
R230	T327	T328	punct	[,8
R231	T328	T304	parataxis	8,are
R232	T329	T328	nummod	7,8
R233	T330	T328	punct	",",8
R234	T331	T328	punct	],8
R235	T332	T286	punct	.,offers
R24	T62	T60	pobj	repair,in
R241	T437	T438	prep	Following,provided
R242	T439	T440	det	the,discovery
R243	T440	T437	pobj	discovery,Following
R244	T441	T440	compound	landmark,discovery
R245	T442	T440	prep	by,discovery
R246	T443	T442	pobj	Scully,by
R247	T444	T445	advmod	et,al
R248	T445	T443	advmod	al,Scully
R249	T446	T447	mark	that,colocalizes
R25	T63	T46	punct	.,provide
R250	T447	T440	acl	colocalizes,discovery
R251	T448	T449	det	the,RAD51
R252	T449	T447	nsubj	RAD51,colocalizes
R253	T450	T449	amod	homologous,RAD51
R254	T451	T449	compound	recombinase,RAD51
R255	T452	T447	prep	at,colocalizes
R256	T453	T454	amod	subnuclear,sites
R257	T454	T452	pobj	sites,at
R258	T455	T447	prep	with,colocalizes
R259	T456	T455	pobj	BRCA1,with
R26	T65	T66	nsubj	Moynahan,provide
R260	T457	T458	punct	[,9
R261	T458	T447	parataxis	9,colocalizes
R262	T459	T458	punct	],9
R263	T460	T438	punct	", ",provided
R264	T461	T462	det	a,number
R265	T462	T438	nsubj	number,provided
R266	T463	T462	prep	of,number
R267	T464	T465	amod	additional,results
R268	T465	T463	pobj	results,of
R269	T466	T438	aux	have,provided
R27	T67	T68	advmod	et,al
R270	T467	T438	dobj	evidence,provided
R271	T468	T469	mark	that,involved
R272	T469	T467	acl	involved,evidence
R273	T470	T471	preconj	both,BRCA1
R274	T471	T469	nsubjpass	BRCA1,involved
R275	T472	T471	cc	and,BRCA1
R276	T473	T471	conj	BRCA2,BRCA1
R277	T474	T469	auxpass	are,involved
R278	T475	T469	prep	in,involved
R279	T476	T477	amod	recombinational,repair
R28	T68	T65	advmod	al,Moynahan
R280	T477	T475	pobj	repair,in
R281	T478	T477	prep	of,repair
R282	T479	T480	compound	DNA,damage
R283	T480	T478	pobj	damage,of
R284	T481	T438	punct	.,provided
R285	T483	T484	nsubj	BRCA1,form
R286	T485	T483	cc	and,BRCA1
R287	T486	T483	conj	BRCA2,BRCA1
R288	T487	T488	amod	discrete,foci
R289	T488	T484	dobj	foci,form
R29	T69	T70	amod	genetic,data
R290	T489	T488	amod	nuclear,foci
R291	T490	T484	prep	during,form
R292	T491	T492	det	the,phase
R293	T492	T490	pobj	phase,during
R294	T493	T492	compound	S,phase
R295	T494	T490	cc	and,during
R296	T495	T490	conj	after,during
R297	T496	T495	pobj	exposure,after
R298	T497	T496	prep	to,exposure
R299	T498	T499	npadvmod	DNA,damaging
R3	T38	T36	cc	and,BRCA2
R30	T70	T66	dobj	data,provide
R300	T499	T500	amod	damaging,agents
R301	T500	T497	pobj	agents,to
R302	T501	T502	punct	[,11
R303	T502	T484	parataxis	11,form
R304	T503	T502	nummod	9,11
R305	T504	T502	punct	",",11
R306	T505	T502	nummod	10,11
R307	T506	T502	punct	",",11
R308	T507	T502	punct	],11
R309	T508	T484	punct	.,form
R31	T71	T70	acl	indicating,data
R310	T510	T511	det	These,foci
R311	T511	T512	nsubj	foci,are
R312	T513	T512	advmod	probably,are
R313	T514	T512	attr	sites,are
R314	T515	T514	prep	of,sites
R315	T516	T515	pobj	repair,of
R316	T517	T516	prep	of,repair
R317	T518	T519	amod	spontaneous,damage
R318	T519	T517	pobj	damage,of
R319	T520	T518	cc	and,spontaneous
R32	T72	T73	det	a,requirement
R320	T521	T518	conj	induced,spontaneous
R321	T522	T519	compound	DNA,damage
R322	T523	T524	punct	[,14
R323	T524	T512	parataxis	14,are
R324	T525	T524	nummod	12,14
R325	T526	T524	punct	",",14
R326	T527	T524	nummod	13,14
R327	T528	T524	punct	",",14
R328	T529	T524	punct	],14
R329	T530	T512	punct	.,are
R33	T73	T71	dobj	requirement,indicating
R330	T532	T533	compound	Cell,lines
R331	T533	T534	nsubj	lines,are
R332	T535	T533	amod	defective,lines
R333	T536	T535	prep	in,defective
R334	T537	T538	preconj	either,BRCA1
R335	T538	T536	pobj	BRCA1,in
R336	T539	T538	cc	or,BRCA1
R337	T540	T538	conj	BRCA2,BRCA1
R338	T541	T534	acomp	sensitive,are
R339	T542	T541	prep	to,sensitive
R34	T74	T73	prep	for,requirement
R340	T543	T544	amod	damaging,agents
R341	T544	T542	pobj	agents,to
R342	T545	T546	dep	that,form
R343	T546	T544	relcl	form,agents
R344	T547	T548	amod	double,strand
R345	T548	T550	amod	strand,breaks
R346	T549	T548	punct	-,strand
R347	T550	T546	dobj	breaks,form
R348	T551	T550	punct	(,breaks
R349	T552	T550	appos	DSBs,breaks
R35	T75	T74	pobj	BRCA2,for
R350	T553	T534	punct	),are
R351	T554	T534	punct	", ",are
R352	T555	T556	mark	as,are
R353	T556	T534	advcl	are,are
R354	T557	T558	amod	other,lines
R355	T558	T556	nsubj	lines,are
R356	T559	T558	compound	cell,lines
R357	T560	T558	amod	defective,lines
R358	T561	T560	prep	in,defective
R359	T562	T563	amod	recombinational,repair
R36	T76	T73	prep	in,requirement
R360	T563	T561	pobj	repair,in
R361	T564	T565	punct	(,reviewed
R362	T565	T534	parataxis	reviewed,are
R363	T566	T565	prep	in,reviewed
R364	T567	T566	punct	[,in
R365	T568	T566	pobj	15,in
R366	T569	T566	punct	],in
R367	T570	T565	punct	),reviewed
R368	T571	T534	punct	.,are
R369	T573	T574	nsubj	BRCA2,interacts
R37	T77	T78	amod	homology,dependent
R370	T575	T574	prep	with,interacts
R371	T576	T577	det	the,recombinase
R372	T577	T575	pobj	recombinase,with
R373	T578	T577	compound	RAD51,recombinase
R374	T579	T574	prep	via,interacts
R375	T580	T581	amod	direct,contacts
R376	T581	T579	pobj	contacts,via
R377	T582	T583	compound	protein,protein
R378	T583	T581	compound	protein,contacts
R379	T584	T583	punct	-,protein
R38	T78	T80	amod	dependent,recombinational
R380	T585	T586	punct	[,19
R381	T586	T574	parataxis	19,interacts
R382	T587	T586	nummod	16,19
R383	T588	T586	punct	",",19
R384	T589	T586	nummod	17,19
R385	T590	T586	punct	",",19
R386	T591	T586	nummod	18,19
R387	T592	T586	punct	",",19
R388	T593	T586	punct	],19
R389	T594	T574	punct	.,interacts
R39	T79	T78	punct	-,dependent
R390	T596	T597	amod	Biochemical,analysis
R391	T597	T598	nsubj	analysis,showed
R392	T599	T598	advmod	also,showed
R393	T600	T598	dobj	interaction,showed
R394	T601	T600	prep	between,interaction
R395	T602	T601	pobj	BRCA1,between
R396	T603	T602	cc	and,BRCA1
R397	T604	T602	conj	RAD51,BRCA1
R398	T605	T598	punct	", ",showed
R399	T606	T607	mark	although,been
R4	T39	T40	amod	homologous,recombination
R40	T80	T82	amod	recombinational,repair
R400	T607	T598	advcl	been,showed
R401	T608	T609	det	these,interactions
R402	T609	T607	nsubj	interactions,been
R403	T610	T609	amod	detected,interactions
R404	T611	T607	aux	may,been
R405	T612	T607	aux	have,been
R406	T613	T607	acomp	indirect,been
R407	T614	T615	punct	[,9
R408	T615	T598	parataxis	9,showed
R409	T616	T615	punct	],9
R41	T81	T80	punct	(,recombinational
R410	T617	T598	punct	.,showed
R411	T619	T620	det	The,repeats
R412	T620	T622	nsubj	repeats,are
R413	T621	T620	compound	BRC,repeats
R414	T623	T620	prep	of,repeats
R415	T624	T623	pobj	BRCA2,of
R416	T625	T622	acomp	responsible,are
R417	T626	T625	prep	for,responsible
R418	T627	T628	amod	direct,interaction
R419	T628	T626	pobj	interaction,for
R42	T82	T76	pobj	repair,in
R420	T629	T628	compound	RAD51,interaction
R421	T630	T622	punct	.,are
R422	T632	T633	nsubj	Cells,fail
R423	T634	T632	acl	lacking,Cells
R424	T635	T636	compound	BRCA1,2
R425	T636	T634	dobj	2,lacking
R426	T637	T636	punct	/,2
R427	T638	T639	aux	to,form
R428	T639	T633	xcomp	form,fail
R429	T640	T641	npadvmod	damage,induced
R43	T83	T82	punct	),repair
R430	T641	T643	amod	induced,RAD51
R431	T642	T641	punct	-,induced
R432	T643	T645	compound	RAD51,foci
R433	T644	T643	amod	subnuclear,RAD51
R434	T645	T639	dobj	foci,form
R435	T646	T639	prep	with,form
R436	T647	T648	amod	normal,efficiency
R437	T648	T646	pobj	efficiency,with
R438	T649	T633	punct	", ",fail
R439	T650	T633	advcl	suggesting,fail
R44	T84	T82	prep	of,repair
R440	T651	T652	mark	that,required
R441	T652	T650	ccomp	required,suggesting
R442	T653	T654	det	these,proteins
R443	T654	T652	nsubjpass	proteins,required
R444	T655	T652	auxpass	are,required
R445	T656	T652	prep	for,required
R446	T657	T658	det	the,formation
R447	T658	T656	pobj	formation,for
R448	T659	T658	prep	of,formation
R449	T660	T661	compound	recombinase,complexes
R45	T85	T86	nmod	DNA,breaks
R450	T661	T659	pobj	complexes,of
R451	T662	T658	prep	at,formation
R452	T663	T664	det	the,sites
R453	T664	T662	pobj	sites,at
R454	T665	T664	prep	of,sites
R455	T666	T667	compound	DNA,damage
R456	T667	T665	pobj	damage,of
R457	T668	T669	punct	[,21
R458	T669	T633	parataxis	21,fail
R459	T670	T669	nummod	20,21
R46	T86	T84	pobj	breaks,of
R460	T671	T669	punct	",",21
R461	T672	T669	punct	],21
R462	T673	T633	punct	.,fail
R463	T675	T676	advmod	Finally,shown
R464	T677	T676	punct	", ",shown
R465	T678	T679	amod	genetic,measurements
R466	T679	T676	nsubj	measurements,shown
R467	T680	T679	prep	of,measurements
R468	T681	T682	compound	recombination,frequency
R469	T682	T680	pobj	frequency,of
R47	T87	T88	amod	double,strand
R470	T683	T676	aux	have,shown
R471	T684	T685	mark	that,are
R472	T685	T676	ccomp	are,shown
R473	T686	T687	nmod	Brca1,cells
R474	T687	T685	nsubj	cells,are
R475	T688	T686	punct	-,Brca1
R476	T689	T686	punct	/,Brca1
R477	T690	T686	punct	-,Brca1
R478	T691	T692	amod	embryonic,stem
R479	T692	T687	nmod	stem,cells
R48	T88	T86	compound	strand,breaks
R480	T693	T692	punct	(,stem
R481	T694	T692	appos	ES,stem
R482	T695	T687	punct	),cells
R483	T696	T685	acomp	deficient,are
R484	T697	T696	prep	in,deficient
R485	T698	T699	amod	recombinational,repair
R486	T699	T697	pobj	repair,in
R487	T700	T699	compound	DSB,repair
R488	T701	T702	punct	[,23
R489	T702	T676	parataxis	23,shown
R49	T89	T88	punct	-,strand
R490	T703	T702	nummod	22,23
R491	T704	T702	punct	",",23
R492	T705	T702	punct	],23
R493	T706	T676	punct	.,shown
R494	T708	T709	det	The,similarity
R495	T709	T710	nsubj	similarity,led
R496	T711	T709	prep	between,similarity
R497	T712	T711	pobj	phenotypes,between
R498	T713	T712	acl	associated,phenotypes
R499	T714	T713	prep	with,associated
R5	T40	T36	conj	recombination,BRCA2
R50	T90	T66	punct	.,provide
R500	T715	T716	nmod	BRCA1,deficiency
R501	T716	T714	pobj	deficiency,with
R502	T717	T715	cc	and,BRCA1
R503	T718	T715	conj	BRCA2,BRCA1
R504	T719	T709	punct	", ",similarity
R505	T720	T721	advmod	together,with
R506	T721	T709	prep	with,similarity
R507	T722	T721	pobj	data,with
R508	T723	T722	acl	showing,data
R509	T724	T725	det	a,effect
R51	T92	T93	det	The,paper
R510	T725	T723	dobj	effect,showing
R511	T726	T725	amod	similar,effect
R512	T727	T725	prep	of,effect
R513	T728	T729	compound	DNA,damage
R514	T729	T727	pobj	damage,of
R515	T730	T729	prep	on,damage
R516	T731	T730	pobj	distribution,on
R517	T732	T731	prep	of,distribution
R518	T733	T732	pobj	BRCA1,of
R519	T734	T733	cc	and,BRCA1
R52	T93	T95	nsubj	paper,begins
R520	T735	T733	conj	BRCA2,BRCA1
R521	T736	T731	prep	in,distribution
R522	T737	T738	npadvmod	repair,proficient
R523	T738	T740	amod	proficient,cells
R524	T739	T738	punct	-,proficient
R525	T740	T736	pobj	cells,in
R526	T741	T710	punct	", ",led
R527	T742	T710	prep	to,led
R528	T743	T744	det	the,hypothesis
R529	T744	T742	pobj	hypothesis,to
R53	T94	T93	amod	second,paper
R530	T745	T746	mark	that,required
R531	T746	T744	acl	required,hypothesis
R532	T747	T746	nsubjpass	BRCA2,required
R533	T748	T747	punct	", ",BRCA2
R534	T749	T747	prep	like,BRCA2
R535	T750	T749	pobj	BRCA1,like
R536	T751	T746	punct	", ",required
R537	T752	T746	auxpass	is,required
R538	T753	T746	prep	for,required
R539	T754	T755	amod	efficient,repair
R54	T96	T93	punct	", ",paper
R540	T755	T753	pobj	repair,for
R541	T756	T755	amod	recombinational,repair
R542	T757	T710	punct	.,led
R543	T759	T760	det	The,paper
R544	T760	T761	nsubj	paper,provides
R545	T762	T760	prep	by,paper
R546	T763	T762	pobj	Moynahan,by
R547	T764	T765	advmod	et,al
R548	T765	T763	advmod	al,Moynahan
R549	T766	T767	punct	[,24
R55	T97	T93	prep	by,paper
R550	T767	T760	parataxis	24,paper
R551	T768	T767	punct	],24
R552	T769	T770	amod	important,support
R553	T770	T761	dobj	support,provides
R554	T771	T761	dative	for,provides
R555	T772	T773	det	this,hypothesis
R556	T773	T771	pobj	hypothesis,for
R557	T774	T761	punct	.,provides
R56	T98	T97	pobj	Davies,by
R564	T1002	T1003	nsubj	Pierce,designed
R565	T1004	T1005	advmod	et,al
R566	T1005	T1002	advmod	al,Pierce
R567	T1006	T1003	aux	have,designed
R568	T1007	T1008	det	a,set
R569	T1008	T1003	dobj	set,designed
R57	T99	T100	advmod	et,al
R570	T1009	T1008	prep	of,set
R571	T1010	T1011	compound	recombination,substrates
R572	T1011	T1009	pobj	substrates,of
R573	T1012	T1003	prep	for,designed
R574	T1013	T1012	pcomp	measuring,for
R575	T1014	T1015	det	the,level
R576	T1015	T1013	dobj	level,measuring
R577	T1016	T1015	prep	of,level
R578	T1017	T1018	amod	homologous,recombination
R579	T1018	T1016	pobj	recombination,of
R58	T100	T98	advmod	al,Davies
R580	T1019	T1020	advmod	in,vivo
R581	T1020	T1013	advmod	vivo,measuring
R582	T1021	T1022	punct	(,Fig.
R583	T1022	T1003	parataxis	Fig.,designed
R584	T1023	T1022	nummod	1,Fig.
R585	T1024	T1022	punct	),Fig.
R586	T1025	T1026	punct	[,25
R587	T1026	T1003	parataxis	25,designed
R588	T1027	T1026	punct	],25
R589	T1028	T1003	punct	.,designed
R59	T101	T95	punct	", ",begins
R590	T1030	T1031	det	The,substrate
R591	T1031	T1033	nsubj	substrate,contains
R592	T1032	T1031	compound	DNA,substrate
R593	T1034	T1035	det	a,pair
R594	T1035	T1033	dobj	pair,contains
R595	T1036	T1035	prep	of,pair
R596	T1037	T1038	amod	mutated,genes
R597	T1038	T1036	pobj	genes,of
R598	T1039	T1038	compound	GFP,genes
R599	T1040	T1041	punct	(,encodes
R6	T43	T44	nummod	Two,papers
R60	T102	T103	aux	to,address
R600	T1041	T1035	parataxis	encodes,pair
R601	T1042	T1041	nsubj	GFP,encodes
R602	T1043	T1044	det	the,protein
R603	T1044	T1041	dobj	protein,encodes
R604	T1045	T1046	advmod	easily,detected
R605	T1046	T1044	amod	detected,protein
R606	T1047	T1044	amod	green,protein
R607	T1048	T1044	amod	fluorescent,protein
R608	T1049	T1041	punct	),encodes
R609	T1050	T1035	punct	", ",pair
R61	T103	T95	xcomp	address,begins
R610	T1051	T1052	dep	one,contains
R611	T1052	T1035	relcl	contains,pair
R612	T1053	T1051	prep	of,one
R613	T1054	T1053	pobj	which,of
R614	T1055	T1056	det	a,site
R615	T1056	T1052	dobj	site,contains
R616	T1057	T1056	compound	restriction,site
R617	T1058	T1056	prep	for,site
R618	T1059	T1060	compound	I,SceI
R619	T1060	T1058	pobj	SceI,for
R62	T104	T105	det	the,mechanism
R620	T1061	T1060	punct	-,SceI
R621	T1062	T1060	punct	", ",SceI
R622	T1063	T1064	det	a,endonuclease
R623	T1064	T1060	appos	endonuclease,SceI
R624	T1065	T1066	compound	yeast,intron
R625	T1066	T1067	npadvmod	intron,encoded
R626	T1067	T1064	amod	encoded,endonuclease
R627	T1068	T1064	prep	with,endonuclease
R628	T1069	T1070	det	an,site
R629	T1070	T1068	pobj	site,with
R63	T105	T103	dobj	mechanism,address
R630	T1071	T1072	nummod	18,pair
R631	T1072	T1070	compound	pair,site
R632	T1073	T1072	compound	base,pair
R633	T1074	T1070	compound	recognition,site
R634	T1075	T1033	punct	.,contains
R635	T1077	T1078	amod	Transient,transfection
R636	T1078	T1079	nsubj	transfection,results
R637	T1080	T1078	prep	of,transfection
R638	T1081	T1082	det	an,vector
R639	T1082	T1080	pobj	vector,of
R64	T106	T107	prep	through,makes
R640	T1083	T1084	compound	I,SceI
R641	T1084	T1082	compound	SceI,vector
R642	T1085	T1084	punct	-,SceI
R643	T1086	T1082	compound	expression,vector
R644	T1087	T1079	prep	in,results
R645	T1088	T1089	det	the,production
R646	T1089	T1087	pobj	production,in
R647	T1090	T1089	prep	of,production
R648	T1091	T1092	det	a,DSB
R649	T1092	T1090	pobj	DSB,of
R65	T107	T105	relcl	makes,mechanism
R650	T1093	T1089	prep	in,production
R651	T1094	T1095	det	the,copy
R652	T1095	T1093	pobj	copy,in
R653	T1096	T1095	amod	first,copy
R654	T1097	T1095	amod	mutated,copy
R655	T1098	T1095	prep	of,copy
R656	T1099	T1098	pobj	GFP,of
R657	T1100	T1079	punct	.,results
R658	T1102	T1103	nsubj	One,invade
R659	T1104	T1102	cc	or,One
R66	T108	T106	pobj	which,through
R660	T1105	T1106	nmod	both,ends
R661	T1106	T1102	conj	ends,One
R662	T1107	T1106	compound	DNA,ends
R663	T1108	T1102	acl	formed,One
R664	T1109	T1108	agent	by,formed
R665	T1110	T1111	det	the,break
R666	T1111	T1109	pobj	break,by
R667	T1112	T1103	punct	(,invade
R668	T1113	T1103	dep	s,invade
R669	T1114	T1103	punct	),invade
R67	T109	T107	nsubj	BRCA2,makes
R670	T1115	T1116	det	the,sequence
R671	T1116	T1103	dobj	sequence,invade
R672	T1117	T1116	amod	homologous,sequence
R673	T1118	T1116	prep	in,sequence
R674	T1119	T1120	det	the,copy
R675	T1120	T1118	pobj	copy,in
R676	T1121	T1120	amod	second,copy
R677	T1122	T1123	amod	mutant,GFP
R678	T1123	T1120	compound	GFP,copy
R679	T1124	T1103	punct	", ",invade
R68	T110	T111	poss	its,contribution
R680	T1125	T1103	advcl	resulting,invade
R681	T1126	T1125	prep	in,resulting
R682	T1127	T1126	pobj	repair,in
R683	T1128	T1127	prep	of,repair
R684	T1129	T1130	det	the,DSB
R685	T1130	T1128	pobj	DSB,of
R686	T1131	T1127	prep	via,repair
R687	T1132	T1133	det	a,event
R688	T1133	T1131	pobj	event,via
R689	T1134	T1135	npadvmod	homology,mediated
R69	T111	T107	dobj	contribution,makes
R690	T1135	T1133	amod	mediated,event
R691	T1136	T1135	punct	-,mediated
R692	T1137	T1138	compound	gene,conversion
R693	T1138	T1133	compound	conversion,event
R694	T1139	T1103	punct	.,invade
R695	T1141	T1142	det	The,configuration
R696	T1142	T1143	nsubj	configuration,is
R697	T1144	T1142	prep	of,configuration
R698	T1145	T1146	det	the,construct
R699	T1146	T1144	pobj	construct,of
R7	T44	T46	nsubj	papers,provide
R70	T112	T111	prep	to,contribution
R700	T1147	T1146	compound	GFP,construct
R701	T1148	T1143	acomp	such,is
R702	T1149	T1150	mark	that,results
R703	T1150	T1148	ccomp	results,such
R704	T1151	T1152	npadvmod	homology,mediated
R705	T1152	T1154	amod	mediated,repair
R706	T1153	T1152	punct	-,mediated
R707	T1154	T1150	nsubj	repair,results
R708	T1155	T1150	advmod	often,results
R709	T1156	T1150	prep	in,results
R71	T113	T114	amod	recombinational,repair
R710	T1157	T1158	det	the,formation
R711	T1158	T1156	pobj	formation,in
R712	T1159	T1158	prep	of,formation
R713	T1160	T1161	det	a,copy
R714	T1161	T1159	pobj	copy,of
R715	T1162	T1161	amod	functional,copy
R716	T1163	T1161	prep	of,copy
R717	T1164	T1163	pobj	GFP,of
R718	T1165	T1143	punct	.,is
R719	T1167	T1168	amod	Such,events
R72	T114	T112	pobj	repair,to
R720	T1168	T1169	nsubjpass	events,detected
R721	T1170	T1169	aux	can,detected
R722	T1171	T1169	auxpass	be,detected
R723	T1172	T1169	prep	by,detected
R724	T1173	T1174	npadvmod	fluorescence,activated
R725	T1174	T1176	amod	activated,sorting
R726	T1175	T1174	punct	-,activated
R727	T1176	T1178	compound	sorting,analysis
R728	T1177	T1176	compound	cell,sorting
R729	T1178	T1172	pobj	analysis,by
R73	T115	T95	punct	.,begins
R730	T1179	T1169	prep	by,detected
R731	T1180	T1179	pobj	virtue,by
R732	T1181	T1180	prep	of,virtue
R733	T1182	T1183	poss	their,expression
R734	T1183	T1181	pobj	expression,of
R735	T1184	T1183	prep	of,expression
R736	T1185	T1184	pobj	GFP,of
R737	T1186	T1169	punct	.,detected
R738	T1188	T1189	nsubj	Moynahan,used
R739	T1190	T1191	advmod	et,al
R74	T117	T118	nsubj	BRCA2,appears
R740	T1191	T1188	advmod	al,Moynahan
R741	T1192	T1193	punct	[,24
R742	T1193	T1188	parataxis	24,Moynahan
R743	T1194	T1193	punct	],24
R744	T1195	T1189	aux	have,used
R745	T1196	T1197	predet	such,substrate
R746	T1197	T1189	dobj	substrate,used
R747	T1198	T1197	det	a,substrate
R748	T1199	T1200	compound	GFP,recombination
R749	T1200	T1197	compound	recombination,substrate
R75	T119	T120	aux	to,function
R750	T1201	T1202	aux	to,demonstrate
R751	T1202	T1189	advcl	demonstrate,used
R752	T1203	T1204	mark	that,are
R753	T1204	T1202	ccomp	are,demonstrate
R754	T1205	T1204	nsubj	cells,are
R755	T1206	T1205	prep	with,cells
R756	T1207	T1208	amod	defective,protein
R757	T1208	T1206	pobj	protein,with
R758	T1209	T1208	compound	BRCA2,protein
R759	T1210	T1204	acomp	deficient,are
R76	T120	T118	xcomp	function,appears
R760	T1211	T1210	prep	in,deficient
R761	T1212	T1213	poss	their,ability
R762	T1213	T1211	pobj	ability,in
R763	T1214	T1215	aux	to,repair
R764	T1215	T1213	acl	repair,ability
R765	T1216	T1217	det	the,DSB
R766	T1217	T1215	dobj	DSB,repair
R767	T1218	T1219	compound	I,SceI
R768	T1219	T1221	npadvmod	SceI,induced
R769	T1220	T1219	punct	-,SceI
R77	T121	T120	prep	in,function
R770	T1221	T1217	amod	induced,DSB
R771	T1222	T1221	punct	-,induced
R772	T1223	T1215	prep	through,repair
R773	T1224	T1225	amod	homologous,recombination
R774	T1225	T1223	pobj	recombination,through
R775	T1226	T1189	punct	.,used
R776	T1228	T1229	nsubj	Expression,resulted
R777	T1230	T1228	prep	of,Expression
R778	T1231	T1232	compound	I,SceI
R779	T1232	T1230	pobj	SceI,of
R78	T122	T121	pobj	recombination,in
R780	T1233	T1232	punct	-,SceI
R781	T1234	T1229	prep	in,resulted
R782	T1235	T1236	quantmod	1,1400
R783	T1236	T1239	nummod	1400,cells
R784	T1237	T1236	quantmod	out,1400
R785	T1238	T1236	quantmod	of,1400
R786	T1239	T1240	nsubj	cells,producing
R787	T1240	T1234	pcomp	producing,in
R788	T1241	T1240	dobj	GFP,producing
R789	T1242	T1240	prep	via,producing
R79	T123	T120	prep	via,function
R790	T1243	T1244	amod	homologous,recombination
R791	T1244	T1242	pobj	recombination,via
R792	T1245	T1244	prep	in,recombination
R793	T1246	T1247	det	the,line
R794	T1247	T1245	pobj	line,in
R795	T1248	T1249	amod	human,cell
R796	T1249	T1247	compound	cell,line
R797	T1250	T1249	amod	pancreatic,cell
R798	T1251	T1249	compound	tumor,cell
R799	T1252	T1247	appos	CAPAN,line
R8	T45	T44	amod	recent,papers
R80	T124	T123	pobj	interactions,via
R800	T1253	T1252	punct	-,CAPAN
R801	T1254	T1252	nummod	1,CAPAN
R802	T1255	T1229	punct	.,resulted
R803	T1257	T1258	nmod	CAPAN,cells
R804	T1258	T1261	nsubj	cells,carry
R805	T1259	T1257	punct	-,CAPAN
R806	T1260	T1257	nummod	1,CAPAN
R807	T1262	T1263	det	a,deletion
R808	T1263	T1261	dobj	deletion,carry
R809	T1264	T1263	prep	of,deletion
R81	T125	T124	prep	with,interactions
R810	T1265	T1264	pobj	BRCA2,of
R811	T1266	T1261	prep	on,carry
R812	T1267	T1268	nummod	one,homolog
R813	T1268	T1266	pobj	homolog,on
R814	T1269	T1261	cc	and,carry
R815	T1270	T1261	conj	codes,carry
R816	T1271	T1270	prep	for,codes
R817	T1272	T1273	det	a,protein
R818	T1273	T1271	pobj	protein,for
R819	T1274	T1273	acl	truncated,protein
R82	T126	T127	det	the,recombinase
R820	T1275	T1274	prep	at,truncated
R821	T1276	T1277	amod	amino,acid
R822	T1277	T1275	pobj	acid,at
R823	T1278	T1277	appos	1981,acid
R824	T1279	T1270	prep	on,codes
R825	T1280	T1281	det	the,homolog
R826	T1281	T1279	pobj	homolog,on
R827	T1282	T1281	amod	other,homolog
R828	T1283	T1261	punct	.,carry
R829	T1285	T1286	det	The,authors
R83	T127	T125	pobj	recombinase,with
R830	T1286	T1287	nsubj	authors,indicate
R831	T1288	T1289	mark	that,is
R832	T1289	T1287	ccomp	is,indicate
R833	T1290	T1291	det	the,level
R834	T1291	T1289	nsubj	level,is
R835	T1292	T1291	prep	of,level
R836	T1293	T1294	compound	I,SceI
R837	T1294	T1296	npadvmod	SceI,induced
R838	T1295	T1294	punct	-,SceI
R839	T1296	T1298	amod	induced,recombination
R84	T128	T127	amod	major,recombinase
R840	T1297	T1296	punct	-,induced
R841	T1298	T1292	pobj	recombination,of
R842	T1299	T1291	prep	in,level
R843	T1300	T1299	pobj	CAPAN,in
R844	T1301	T1300	punct	-,CAPAN
R845	T1302	T1300	nummod	1,CAPAN
R846	T1303	T1304	quantmod	over,100-fold
R847	T1304	T1305	advmod	100-fold,less
R848	T1305	T1289	acomp	less,is
R849	T1306	T1305	prep	than,less
R85	T129	T127	amod	eukaryotic,recombinase
R850	T1307	T1306	pobj	that,than
R851	T1308	T1307	acl	seen,that
R852	T1309	T1308	advcl	using,seen
R853	T1310	T1311	amod	other,BRCA2+
R854	T1311	T1313	amod	BRCA2+,lines
R855	T1312	T1311	punct	(,BRCA2+
R856	T1313	T1309	dobj	lines,using
R857	T1314	T1313	punct	),lines
R858	T1315	T1316	amod	human,tumor
R859	T1316	T1317	compound	tumor,cell
R86	T130	T127	appos	RAD51,recombinase
R860	T1317	T1313	compound	cell,lines
R861	T1318	T1287	punct	.,indicate
R862	T1320	T1321	det	The,lines
R863	T1321	T1323	nsubj	lines,are
R864	T1322	T1321	amod	different,lines
R865	T1324	T1321	acl	examined,lines
R866	T1325	T1323	punct	", ",are
R867	T1326	T1323	advmod	however,are
R868	T1327	T1323	punct	", ",are
R869	T1328	T1329	advmod	very,likely
R87	T131	T132	punct	[,3
R870	T1329	T1323	acomp	likely,are
R871	T1330	T1331	aux	to,differ
R872	T1331	T1329	xcomp	differ,likely
R873	T1332	T1331	advmod	genetically,differ
R874	T1333	T1331	prep	from,differ
R875	T1334	T1335	nmod	CAPAN,cells
R876	T1335	T1333	pobj	cells,from
R877	T1336	T1334	punct	-,CAPAN
R878	T1337	T1334	nummod	1,CAPAN
R879	T1338	T1339	preconj	not,at
R88	T132	T118	parataxis	3,appears
R880	T1339	T1331	prep	at,differ
R881	T1340	T1338	advmod	only,not
R882	T1341	T1342	det	the,locus
R883	T1342	T1339	pobj	locus,at
R884	T1343	T1342	compound	BRCA2,locus
R885	T1344	T1339	punct	", ",at
R886	T1345	T1339	cc	but,at
R887	T1346	T1345	advmod	also,but
R888	T1347	T1339	conj	at,at
R889	T1348	T1349	det	a,number
R89	T133	T132	nummod	1,3
R890	T1349	T1347	pobj	number,at
R891	T1350	T1351	advmod	very,large
R892	T1351	T1349	amod	large,number
R893	T1352	T1349	prep	of,number
R894	T1353	T1354	amod	additional,loci
R895	T1354	T1352	pobj	loci,of
R896	T1355	T1323	punct	.,are
R897	T1357	T1358	nsubj	This,raises
R898	T1359	T1360	det	the,possibility
R899	T1360	T1358	dobj	possibility,raises
R9	T47	T48	amod	new,evidence
R90	T134	T132	punct	",",3
R900	T1361	T1362	mark	that,be
R901	T1362	T1360	acl	be,possibility
R902	T1363	T1362	nsubj	some,be
R903	T1364	T1363	cc	or,some
R904	T1365	T1366	advmod	even,all
R905	T1366	T1363	conj	all,some
R906	T1367	T1363	prep	of,some
R907	T1368	T1369	det	the,defect
R908	T1369	T1367	pobj	defect,of
R909	T1370	T1369	amod	recombinational,defect
R91	T135	T132	nummod	2,3
R910	T1371	T1369	compound	repair,defect
R911	T1372	T1369	acl	seen,defect
R912	T1373	T1372	prep	in,seen
R913	T1374	T1373	pobj	CAPAN,in
R914	T1375	T1374	punct	-,CAPAN
R915	T1376	T1374	nummod	1,CAPAN
R916	T1377	T1362	aux	could,be
R917	T1378	T1362	prep	due,be
R918	T1379	T1378	pcomp	to,due
R919	T1380	T1378	pobj	mutations,due
R92	T136	T132	punct	",",3
R920	T1381	T1380	prep	at,mutations
R921	T1382	T1383	amod	non-BRCA2,loci
R922	T1383	T1381	pobj	loci,at
R923	T1384	T1358	punct	.,raises
R924	T1386	T1387	mark	While,were
R925	T1387	T1391	advcl	were,argue
R926	T1388	T1387	nsubj	Moynahan,were
R927	T1389	T1390	advmod	et,al
R928	T1390	T1388	advmod	al,Moynahan
R929	T1392	T1387	acomp	careful,were
R93	T137	T132	punct	],3
R930	T1393	T1394	aux	to,point
R931	T1394	T1392	xcomp	point,careful
R932	T1395	T1394	prt	out,point
R933	T1396	T1397	det	this,problem
R934	T1397	T1394	dobj	problem,point
R935	T1398	T1391	punct	", ",argue
R936	T1399	T1400	nummod	two,sets
R937	T1400	T1391	nsubj	sets,argue
R938	T1401	T1400	prep	of,sets
R939	T1402	T1401	pobj	results,of
R94	T138	T118	punct	.,appears
R940	T1403	T1391	prep	against,argue
R941	T1404	T1405	det	the,possibility
R942	T1405	T1403	pobj	possibility,against
R943	T1406	T1407	mark	that,is
R944	T1407	T1405	acl	is,possibility
R945	T1408	T1409	det	the,deficiency
R946	T1409	T1407	nsubj	deficiency,is
R947	T1410	T1409	amod	recombinational,deficiency
R948	T1411	T1409	compound	repair,deficiency
R949	T1412	T1409	prep	of,deficiency
R95	T140	T141	nsubj	We,review
R950	T1413	T1414	nmod	CAPAN,cells
R951	T1414	T1412	pobj	cells,of
R952	T1415	T1413	punct	-,CAPAN
R953	T1416	T1413	nummod	1,CAPAN
R954	T1417	T1418	advmod	completely,independent
R955	T1418	T1407	acomp	independent,is
R956	T1419	T1418	prep	of,independent
R957	T1420	T1421	poss	its,defect
R958	T1421	T1419	pobj	defect,of
R959	T1422	T1421	prep	in,defect
R96	T142	T141	advmod	briefly,review
R960	T1423	T1422	pobj	BRCA2,in
R961	T1424	T1391	punct	.,argue
R962	T1426	T1427	advmod	First,measured
R963	T1428	T1427	punct	", ",measured
R964	T1429	T1427	prep	in,measured
R965	T1430	T1431	det	the,study
R966	T1431	T1429	pobj	study,in
R967	T1432	T1431	amod	same,study
R968	T1433	T1434	punct	[,24
R969	T1434	T1431	parataxis	24,study
R97	T143	T144	det	the,context
R970	T1435	T1434	punct	],24
R971	T1436	T1427	punct	", ",measured
R972	T1437	T1427	nsubjpass	recombination,measured
R973	T1438	T1437	prep	in,recombination
R974	T1439	T1440	det	a,line
R975	T1440	T1438	pobj	line,in
R976	T1441	T1440	compound	mouse,line
R977	T1442	T1440	compound	ES,line
R978	T1443	T1440	compound	cell,line
R979	T1444	T1445	dep	that,expresses
R98	T144	T141	dobj	context,review
R980	T1445	T1440	relcl	expresses,line
R981	T1446	T1447	advmod	only,protein
R982	T1447	T1445	dobj	protein,expresses
R983	T1448	T1447	amod	truncated,protein
R984	T1449	T1447	compound	BRCA2,protein
R985	T1450	T1427	auxpass	was,measured
R986	T1451	T1427	punct	.,measured
R987	T1453	T1454	det	This,line
R988	T1454	T1455	nsubjpass	line,found
R989	T1456	T1455	auxpass	was,found
R99	T145	T146	prep	in,carried
R990	T1457	T1458	aux	to,have
R991	T1458	T1455	xcomp	have,found
R992	T1459	T1460	amod	lower,efficiency
R993	T1460	T1458	dobj	efficiency,have
R994	T1461	T1460	compound	recombination,efficiency
R995	T1462	T1460	prep	than,efficiency
R996	T1463	T1464	amod	isogenic,cells
R997	T1464	T1462	pobj	cells,than
R998	T1465	T1464	acl	expressing,cells
R999	T1466	T1467	amod	full,length

PMC:138691 JSONTXT 7 Projects

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PMC:138691 JSON TXT 7 Projects