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PMC:1382200 / 33495-33924 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T17625 0-3 DT denotes The
T17627 4-13 NN denotes targeting
T17626 14-23 NN denotes construct
T17629 24-27 VBD denotes was
T17628 28-38 VBN denotes linearized
T17630 39-41 IN denotes by
T17631 42-46 NN denotes NotI
T17632 47-56 NN denotes digestion
T17633 57-60 CC denotes and
T17634 61-75 VBN denotes electroporated
T17635 76-80 IN denotes into
T17636 81-83 NN denotes R1
T17638 84-86 NN denotes ES
T17637 87-92 NNS denotes cells
T17639 92-93 . denotes .
T17640 93-294 sentence denotes Cells were plated without feeder layers on gelatin-coated plates and transformed clones were selected in the presence of 200 μg/ml G418 (Gibco- BRL) and 2 μM ganciclovir (Syntex, Inc.) for eight days.
T17641 94-99 NNS denotes Cells
T17643 100-104 VBD denotes were
T17642 105-111 VBN denotes plated
T17644 112-119 IN denotes without
T17645 120-126 NN denotes feeder
T17646 127-133 NNS denotes layers
T17647 134-136 IN denotes on
T17648 137-144 NN denotes gelatin
T17650 144-145 HYPH denotes -
T17649 145-151 VBN denotes coated
T17651 152-158 NNS denotes plates
T17652 159-162 CC denotes and
T17653 163-174 VBN denotes transformed
T17654 175-181 NNS denotes clones
T17656 182-186 VBD denotes were
T17655 187-195 VBN denotes selected
T17657 196-198 IN denotes in
T17658 199-202 DT denotes the
T17659 203-211 NN denotes presence
T17660 212-214 IN denotes of
T17661 215-218 CD denotes 200
T17662 219-221 NN denotes μg
T17664 221-222 SYM denotes /
T17665 222-224 NN denotes ml
T17663 225-229 NN denotes G418
T17666 230-231 -LRB- denotes (
T17668 231-236 NNP denotes Gibco
T17669 236-237 HYPH denotes -
T17667 238-241 NNP denotes BRL
T17670 241-242 -RRB- denotes )
T17671 243-246 CC denotes and
T17672 247-248 CD denotes 2
T17673 249-251 NN denotes μM
T17674 252-263 NN denotes ganciclovir
T17675 264-265 -LRB- denotes (
T17676 265-271 NNP denotes Syntex
T17677 271-273 , denotes ,
T17678 273-277 NNP denotes Inc.
T17679 277-278 -RRB- denotes )
T17680 279-282 IN denotes for
T17681 283-288 CD denotes eight
T17682 289-293 NNS denotes days
T17683 293-294 . denotes .
T17684 294-429 sentence denotes Drug-resistant clones were picked, expanded on gelatin-coated plates, and genotyped by Southern blotting and PCR analysis (see below).
T17685 295-299 NN denotes Drug
T17687 299-300 HYPH denotes -
T17686 300-309 JJ denotes resistant
T17688 310-316 NNS denotes clones
T17690 317-321 VBD denotes were
T17689 322-328 VBN denotes picked
T17691 328-330 , denotes ,
T17692 330-338 VBN denotes expanded
T17693 339-341 IN denotes on
T17694 342-349 NN denotes gelatin
T17696 349-350 HYPH denotes -
T17695 350-356 VBN denotes coated
T17697 357-363 NNS denotes plates
T17698 363-365 , denotes ,
T17699 365-368 CC denotes and
T17700 369-378 VBN denotes genotyped
T17701 379-381 IN denotes by
T17702 382-390 NNP denotes Southern
T17703 391-399 NN denotes blotting
T17704 400-403 CC denotes and
T17705 404-407 NN denotes PCR
T17706 408-416 NN denotes analysis
T17707 417-418 -LRB- denotes (
T17708 418-421 VB denotes see
T17709 422-427 RB denotes below
T17710 427-428 -RRB- denotes )
T17711 428-429 . denotes .
R5275 T17625 T17626 det The,construct
R5276 T17626 T17628 nsubjpass construct,linearized
R5277 T17627 T17626 compound targeting,construct
R5278 T17629 T17628 auxpass was,linearized
R5279 T17630 T17628 agent by,linearized
R5280 T17631 T17632 compound NotI,digestion
R5281 T17632 T17630 pobj digestion,by
R5282 T17633 T17628 cc and,linearized
R5283 T17634 T17628 conj electroporated,linearized
R5284 T17635 T17634 prep into,electroporated
R5285 T17636 T17637 compound R1,cells
R5286 T17637 T17635 pobj cells,into
R5287 T17638 T17637 compound ES,cells
R5288 T17639 T17628 punct .,linearized
R5289 T17641 T17642 nsubjpass Cells,plated
R5290 T17643 T17642 auxpass were,plated
R5291 T17644 T17642 prep without,plated
R5292 T17645 T17646 compound feeder,layers
R5293 T17646 T17644 pobj layers,without
R5294 T17647 T17642 prep on,plated
R5295 T17648 T17649 npadvmod gelatin,coated
R5296 T17649 T17651 amod coated,plates
R5297 T17650 T17649 punct -,coated
R5298 T17651 T17647 pobj plates,on
R5299 T17652 T17642 cc and,plated
R5300 T17653 T17654 amod transformed,clones
R5301 T17654 T17655 nsubjpass clones,selected
R5302 T17655 T17642 conj selected,plated
R5303 T17656 T17655 auxpass were,selected
R5304 T17657 T17655 prep in,selected
R5305 T17658 T17659 det the,presence
R5306 T17659 T17657 pobj presence,in
R5307 T17660 T17659 prep of,presence
R5308 T17661 T17662 nummod 200,μg
R5309 T17662 T17663 nmod μg,G418
R5310 T17663 T17660 pobj G418,of
R5311 T17664 T17665 punct /,ml
R5312 T17665 T17662 prep ml,μg
R5313 T17666 T17667 punct (,BRL
R5314 T17667 T17663 parataxis BRL,G418
R5315 T17668 T17667 compound Gibco,BRL
R5316 T17669 T17667 punct -,BRL
R5317 T17670 T17667 punct ),BRL
R5318 T17671 T17663 cc and,G418
R5319 T17672 T17673 nummod 2,μM
R5320 T17673 T17674 compound μM,ganciclovir
R5321 T17674 T17663 conj ganciclovir,G418
R5322 T17675 T17676 punct (,Syntex
R5323 T17676 T17674 parataxis Syntex,ganciclovir
R5324 T17677 T17676 punct ", ",Syntex
R5325 T17678 T17676 amod Inc.,Syntex
R5326 T17679 T17676 punct ),Syntex
R5327 T17680 T17655 prep for,selected
R5328 T17681 T17682 nummod eight,days
R5329 T17682 T17680 pobj days,for
R5330 T17683 T17655 punct .,selected
R5331 T17685 T17686 npadvmod Drug,resistant
R5332 T17686 T17688 amod resistant,clones
R5333 T17687 T17686 punct -,resistant
R5334 T17688 T17689 nsubjpass clones,picked
R5335 T17690 T17689 auxpass were,picked
R5336 T17691 T17689 punct ", ",picked
R5337 T17692 T17689 conj expanded,picked
R5338 T17693 T17692 prep on,expanded
R5339 T17694 T17695 npadvmod gelatin,coated
R5340 T17695 T17697 amod coated,plates
R5341 T17696 T17695 punct -,coated
R5342 T17697 T17693 pobj plates,on
R5343 T17698 T17692 punct ", ",expanded
R5344 T17699 T17692 cc and,expanded
R5345 T17700 T17692 conj genotyped,expanded
R5346 T17701 T17700 prep by,genotyped
R5347 T17702 T17703 compound Southern,blotting
R5348 T17703 T17701 pobj blotting,by
R5349 T17704 T17703 cc and,blotting
R5350 T17705 T17706 compound PCR,analysis
R5351 T17706 T17703 conj analysis,blotting
R5352 T17707 T17708 punct (,see
R5353 T17708 T17700 parataxis see,genotyped
R5354 T17709 T17708 advmod below,see
R5355 T17710 T17708 punct ),see
R5356 T17711 T17689 punct .,picked