| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T17462 |
0-2 |
NN |
denotes |
ES |
| T17464 |
3-7 |
NN |
denotes |
cell |
| T17463 |
8-15 |
NN |
denotes |
culture |
| T17465 |
15-17 |
, |
denotes |
, |
| T17466 |
17-29 |
NN |
denotes |
transfection |
| T17467 |
30-33 |
CC |
denotes |
and |
| T17468 |
34-43 |
NN |
denotes |
selection |
| T17469 |
44-46 |
IN |
denotes |
of |
| T17470 |
47-55 |
VBN |
denotes |
targeted |
| T17471 |
56-62 |
NNS |
denotes |
clones |
| T17472 |
62-481 |
sentence |
denotes |
Mouse R1 ES cells [49] were maintained on gelatin-coated plates with feeder layers of mouse embryonic fibroblasts at 37°C under 5% CO2 in ES cell medium (DMEM [high glucose] supplemented with 15% fetal bovine serum, 0.1 mM non-essential amino acids, 2 mM glutamine, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 100 U/ml penicillin, 100 μg/ml streptomycin, 25 μg/ml amphotericin, and 1,000 U/ml of ESGro [Chemicon]). |
| T17473 |
63-68 |
NN |
denotes |
Mouse |
| T17475 |
69-71 |
NN |
denotes |
R1 |
| T17476 |
72-74 |
NN |
denotes |
ES |
| T17474 |
75-80 |
NNS |
denotes |
cells |
| T17478 |
81-82 |
-LRB- |
denotes |
[ |
| T17479 |
82-84 |
CD |
denotes |
49 |
| T17480 |
84-85 |
-RRB- |
denotes |
] |
| T17481 |
86-90 |
VBD |
denotes |
were |
| T17477 |
91-101 |
VBN |
denotes |
maintained |
| T17482 |
102-104 |
IN |
denotes |
on |
| T17483 |
105-112 |
NN |
denotes |
gelatin |
| T17485 |
112-113 |
HYPH |
denotes |
- |
| T17484 |
113-119 |
VBN |
denotes |
coated |
| T17486 |
120-126 |
NNS |
denotes |
plates |
| T17487 |
127-131 |
IN |
denotes |
with |
| T17488 |
132-138 |
NN |
denotes |
feeder |
| T17489 |
139-145 |
NNS |
denotes |
layers |
| T17490 |
146-148 |
IN |
denotes |
of |
| T17491 |
149-154 |
NN |
denotes |
mouse |
| T17493 |
155-164 |
JJ |
denotes |
embryonic |
| T17492 |
165-176 |
NNS |
denotes |
fibroblasts |
| T17494 |
177-179 |
IN |
denotes |
at |
| T17495 |
180-182 |
CD |
denotes |
37 |
| T17496 |
182-184 |
NN |
denotes |
°C |
| T17497 |
185-190 |
IN |
denotes |
under |
| T17498 |
191-192 |
CD |
denotes |
5 |
| T17499 |
192-193 |
NN |
denotes |
% |
| T17500 |
194-197 |
NN |
denotes |
CO2 |
| T17501 |
198-200 |
IN |
denotes |
in |
| T17502 |
201-203 |
NN |
denotes |
ES |
| T17504 |
204-208 |
NN |
denotes |
cell |
| T17503 |
209-215 |
NN |
denotes |
medium |
| T17505 |
216-217 |
-LRB- |
denotes |
( |
| T17506 |
217-221 |
NN |
denotes |
DMEM |
| T17507 |
222-223 |
-LRB- |
denotes |
[ |
| T17509 |
223-227 |
JJ |
denotes |
high |
| T17508 |
228-235 |
NN |
denotes |
glucose |
| T17510 |
235-236 |
-RRB- |
denotes |
] |
| T17511 |
237-249 |
VBN |
denotes |
supplemented |
| T17512 |
250-254 |
IN |
denotes |
with |
| T17513 |
255-257 |
CD |
denotes |
15 |
| T17514 |
257-258 |
NN |
denotes |
% |
| T17516 |
259-264 |
JJ |
denotes |
fetal |
| T17517 |
265-271 |
JJ |
denotes |
bovine |
| T17515 |
272-277 |
NN |
denotes |
serum |
| T17518 |
277-279 |
, |
denotes |
, |
| T17519 |
279-282 |
CD |
denotes |
0.1 |
| T17520 |
283-285 |
NN |
denotes |
mM |
| T17522 |
286-299 |
JJ |
denotes |
non-essential |
| T17523 |
300-305 |
NN |
denotes |
amino |
| T17521 |
306-311 |
NNS |
denotes |
acids |
| T17524 |
311-313 |
, |
denotes |
, |
| T17525 |
313-314 |
CD |
denotes |
2 |
| T17526 |
315-317 |
NN |
denotes |
mM |
| T17527 |
318-327 |
NN |
denotes |
glutamine |
| T17528 |
327-329 |
, |
denotes |
, |
| T17529 |
329-330 |
CD |
denotes |
1 |
| T17530 |
331-333 |
NN |
denotes |
mM |
| T17532 |
334-340 |
NN |
denotes |
sodium |
| T17531 |
341-349 |
NN |
denotes |
pyruvate |
| T17533 |
349-351 |
, |
denotes |
, |
| T17534 |
351-354 |
CD |
denotes |
0.1 |
| T17535 |
355-357 |
NN |
denotes |
mM |
| T17537 |
358-359 |
CD |
denotes |
2 |
| T17538 |
359-360 |
HYPH |
denotes |
- |
| T17536 |
360-375 |
NN |
denotes |
mercaptoethanol |
| T17539 |
375-377 |
, |
denotes |
, |
| T17540 |
377-380 |
CD |
denotes |
100 |
| T17541 |
381-382 |
NN |
denotes |
U |
| T17543 |
382-383 |
SYM |
denotes |
/ |
| T17544 |
383-385 |
NN |
denotes |
ml |
| T17542 |
386-396 |
NN |
denotes |
penicillin |
| T17545 |
396-398 |
, |
denotes |
, |
| T17546 |
398-401 |
CD |
denotes |
100 |
| T17547 |
402-404 |
NN |
denotes |
μg |
| T17549 |
404-405 |
SYM |
denotes |
/ |
| T17550 |
405-407 |
NN |
denotes |
ml |
| T17548 |
408-420 |
NN |
denotes |
streptomycin |
| T17551 |
420-422 |
, |
denotes |
, |
| T17552 |
422-424 |
CD |
denotes |
25 |
| T17553 |
425-427 |
NN |
denotes |
μg |
| T17555 |
427-428 |
SYM |
denotes |
/ |
| T17556 |
428-430 |
NN |
denotes |
ml |
| T17554 |
431-443 |
NN |
denotes |
amphotericin |
| T17557 |
443-445 |
, |
denotes |
, |
| T17558 |
445-448 |
CC |
denotes |
and |
| T17559 |
449-454 |
CD |
denotes |
1,000 |
| T17560 |
455-456 |
NN |
denotes |
U |
| T17561 |
456-457 |
SYM |
denotes |
/ |
| T17562 |
457-459 |
NN |
denotes |
ml |
| T17563 |
460-462 |
IN |
denotes |
of |
| T17564 |
463-468 |
NN |
denotes |
ESGro |
| T17565 |
469-470 |
-LRB- |
denotes |
[ |
| T17566 |
470-478 |
NNP |
denotes |
Chemicon |
| T17567 |
478-479 |
-RRB- |
denotes |
] |
| T17568 |
479-480 |
-RRB- |
denotes |
) |
| T17569 |
480-481 |
. |
denotes |
. |
| T17570 |
481-598 |
sentence |
denotes |
Fetal bovine serum from HyClone Laboratories Inc (Logan, Utah) was tested for its ability to support ES cell growth. |
| T17571 |
482-487 |
JJ |
denotes |
Fetal |
| T17573 |
488-494 |
JJ |
denotes |
bovine |
| T17572 |
495-500 |
NN |
denotes |
serum |
| T17575 |
501-505 |
IN |
denotes |
from |
| T17576 |
506-513 |
NNP |
denotes |
HyClone |
| T17578 |
514-526 |
NNP |
denotes |
Laboratories |
| T17577 |
527-530 |
NNP |
denotes |
Inc |
| T17579 |
531-532 |
-LRB- |
denotes |
( |
| T17580 |
532-537 |
NNP |
denotes |
Logan |
| T17581 |
537-539 |
, |
denotes |
, |
| T17582 |
539-543 |
NNP |
denotes |
Utah |
| T17583 |
543-544 |
-RRB- |
denotes |
) |
| T17584 |
545-548 |
VBD |
denotes |
was |
| T17574 |
549-555 |
VBN |
denotes |
tested |
| T17585 |
556-559 |
IN |
denotes |
for |
| T17586 |
560-563 |
PRP$ |
denotes |
its |
| T17587 |
564-571 |
NN |
denotes |
ability |
| T17588 |
572-574 |
TO |
denotes |
to |
| T17589 |
575-582 |
VB |
denotes |
support |
| T17590 |
583-585 |
NN |
denotes |
ES |
| T17591 |
586-590 |
NN |
denotes |
cell |
| T17592 |
591-597 |
NN |
denotes |
growth |
| T17593 |
597-598 |
. |
denotes |
. |
| T17594 |
598-657 |
sentence |
denotes |
Gelatin was from Sigma-Aldrich Canada (Oakville, Ontario). |
| T17595 |
599-606 |
NN |
denotes |
Gelatin |
| T17596 |
607-610 |
VBD |
denotes |
was |
| T17597 |
611-615 |
IN |
denotes |
from |
| T17598 |
616-621 |
NNP |
denotes |
Sigma |
| T17600 |
621-622 |
HYPH |
denotes |
- |
| T17599 |
622-629 |
NNP |
denotes |
Aldrich |
| T17601 |
630-636 |
NNP |
denotes |
Canada |
| T17602 |
637-638 |
-LRB- |
denotes |
( |
| T17603 |
638-646 |
NNP |
denotes |
Oakville |
| T17604 |
646-648 |
, |
denotes |
, |
| T17605 |
648-655 |
NNP |
denotes |
Ontario |
| T17606 |
655-656 |
-RRB- |
denotes |
) |
| T17607 |
656-657 |
. |
denotes |
. |
| T17608 |
657-740 |
sentence |
denotes |
Unless otherwise specified, all other tissue culture reagents were from Gibco-BRL. |
| T17609 |
658-664 |
IN |
denotes |
Unless |
| T17611 |
665-674 |
RB |
denotes |
otherwise |
| T17610 |
675-684 |
VBN |
denotes |
specified |
| T17613 |
684-686 |
, |
denotes |
, |
| T17614 |
686-689 |
DT |
denotes |
all |
| T17616 |
690-695 |
JJ |
denotes |
other |
| T17617 |
696-702 |
NN |
denotes |
tissue |
| T17618 |
703-710 |
NN |
denotes |
culture |
| T17615 |
711-719 |
NNS |
denotes |
reagents |
| T17612 |
720-724 |
VBD |
denotes |
were |
| T17619 |
725-729 |
IN |
denotes |
from |
| T17620 |
730-735 |
NNP |
denotes |
Gibco |
| T17622 |
735-736 |
HYPH |
denotes |
- |
| T17621 |
736-739 |
NNP |
denotes |
BRL |
| T17623 |
739-740 |
. |
denotes |
. |
| T17624 |
740-834 |
sentence |
denotes |
The targeting construct was linearized by NotI digestion and electroporated into R1 ES cells. |
| T17625 |
741-744 |
DT |
denotes |
The |
| T17627 |
745-754 |
NN |
denotes |
targeting |
| T17626 |
755-764 |
NN |
denotes |
construct |
| T17629 |
765-768 |
VBD |
denotes |
was |
| T17628 |
769-779 |
VBN |
denotes |
linearized |
| T17630 |
780-782 |
IN |
denotes |
by |
| T17631 |
783-787 |
NN |
denotes |
NotI |
| T17632 |
788-797 |
NN |
denotes |
digestion |
| T17633 |
798-801 |
CC |
denotes |
and |
| T17634 |
802-816 |
VBN |
denotes |
electroporated |
| T17635 |
817-821 |
IN |
denotes |
into |
| T17636 |
822-824 |
NN |
denotes |
R1 |
| T17638 |
825-827 |
NN |
denotes |
ES |
| T17637 |
828-833 |
NNS |
denotes |
cells |
| T17639 |
833-834 |
. |
denotes |
. |
| T17640 |
834-1035 |
sentence |
denotes |
Cells were plated without feeder layers on gelatin-coated plates and transformed clones were selected in the presence of 200 μg/ml G418 (Gibco- BRL) and 2 μM ganciclovir (Syntex, Inc.) for eight days. |
| T17641 |
835-840 |
NNS |
denotes |
Cells |
| T17643 |
841-845 |
VBD |
denotes |
were |
| T17642 |
846-852 |
VBN |
denotes |
plated |
| T17644 |
853-860 |
IN |
denotes |
without |
| T17645 |
861-867 |
NN |
denotes |
feeder |
| T17646 |
868-874 |
NNS |
denotes |
layers |
| T17647 |
875-877 |
IN |
denotes |
on |
| T17648 |
878-885 |
NN |
denotes |
gelatin |
| T17650 |
885-886 |
HYPH |
denotes |
- |
| T17649 |
886-892 |
VBN |
denotes |
coated |
| T17651 |
893-899 |
NNS |
denotes |
plates |
| T17652 |
900-903 |
CC |
denotes |
and |
| T17653 |
904-915 |
VBN |
denotes |
transformed |
| T17654 |
916-922 |
NNS |
denotes |
clones |
| T17656 |
923-927 |
VBD |
denotes |
were |
| T17655 |
928-936 |
VBN |
denotes |
selected |
| T17657 |
937-939 |
IN |
denotes |
in |
| T17658 |
940-943 |
DT |
denotes |
the |
| T17659 |
944-952 |
NN |
denotes |
presence |
| T17660 |
953-955 |
IN |
denotes |
of |
| T17661 |
956-959 |
CD |
denotes |
200 |
| T17662 |
960-962 |
NN |
denotes |
μg |
| T17664 |
962-963 |
SYM |
denotes |
/ |
| T17665 |
963-965 |
NN |
denotes |
ml |
| T17663 |
966-970 |
NN |
denotes |
G418 |
| T17666 |
971-972 |
-LRB- |
denotes |
( |
| T17668 |
972-977 |
NNP |
denotes |
Gibco |
| T17669 |
977-978 |
HYPH |
denotes |
- |
| T17667 |
979-982 |
NNP |
denotes |
BRL |
| T17670 |
982-983 |
-RRB- |
denotes |
) |
| T17671 |
984-987 |
CC |
denotes |
and |
| T17672 |
988-989 |
CD |
denotes |
2 |
| T17673 |
990-992 |
NN |
denotes |
μM |
| T17674 |
993-1004 |
NN |
denotes |
ganciclovir |
| T17675 |
1005-1006 |
-LRB- |
denotes |
( |
| T17676 |
1006-1012 |
NNP |
denotes |
Syntex |
| T17677 |
1012-1014 |
, |
denotes |
, |
| T17678 |
1014-1018 |
NNP |
denotes |
Inc. |
| T17679 |
1018-1019 |
-RRB- |
denotes |
) |
| T17680 |
1020-1023 |
IN |
denotes |
for |
| T17681 |
1024-1029 |
CD |
denotes |
eight |
| T17682 |
1030-1034 |
NNS |
denotes |
days |
| T17683 |
1034-1035 |
. |
denotes |
. |
| T17684 |
1035-1170 |
sentence |
denotes |
Drug-resistant clones were picked, expanded on gelatin-coated plates, and genotyped by Southern blotting and PCR analysis (see below). |
| T17685 |
1036-1040 |
NN |
denotes |
Drug |
| T17687 |
1040-1041 |
HYPH |
denotes |
- |
| T17686 |
1041-1050 |
JJ |
denotes |
resistant |
| T17688 |
1051-1057 |
NNS |
denotes |
clones |
| T17690 |
1058-1062 |
VBD |
denotes |
were |
| T17689 |
1063-1069 |
VBN |
denotes |
picked |
| T17691 |
1069-1071 |
, |
denotes |
, |
| T17692 |
1071-1079 |
VBN |
denotes |
expanded |
| T17693 |
1080-1082 |
IN |
denotes |
on |
| T17694 |
1083-1090 |
NN |
denotes |
gelatin |
| T17696 |
1090-1091 |
HYPH |
denotes |
- |
| T17695 |
1091-1097 |
VBN |
denotes |
coated |
| T17697 |
1098-1104 |
NNS |
denotes |
plates |
| T17698 |
1104-1106 |
, |
denotes |
, |
| T17699 |
1106-1109 |
CC |
denotes |
and |
| T17700 |
1110-1119 |
VBN |
denotes |
genotyped |
| T17701 |
1120-1122 |
IN |
denotes |
by |
| T17702 |
1123-1131 |
NNP |
denotes |
Southern |
| T17703 |
1132-1140 |
NN |
denotes |
blotting |
| T17704 |
1141-1144 |
CC |
denotes |
and |
| T17705 |
1145-1148 |
NN |
denotes |
PCR |
| T17706 |
1149-1157 |
NN |
denotes |
analysis |
| T17707 |
1158-1159 |
-LRB- |
denotes |
( |
| T17708 |
1159-1162 |
VB |
denotes |
see |
| T17709 |
1163-1168 |
RB |
denotes |
below |
| T17710 |
1168-1169 |
-RRB- |
denotes |
) |
| T17711 |
1169-1170 |
. |
denotes |
. |
| R5122 |
T17462 |
T17463 |
compound |
ES,culture |
| R5123 |
T17464 |
T17463 |
compound |
cell,culture |
| R5124 |
T17465 |
T17463 |
punct |
", ",culture |
| R5125 |
T17466 |
T17463 |
conj |
transfection,culture |
| R5126 |
T17467 |
T17466 |
cc |
and,transfection |
| R5127 |
T17468 |
T17466 |
conj |
selection,transfection |
| R5128 |
T17469 |
T17468 |
prep |
of,selection |
| R5129 |
T17470 |
T17471 |
amod |
targeted,clones |
| R5130 |
T17471 |
T17469 |
pobj |
clones,of |
| R5131 |
T17473 |
T17474 |
compound |
Mouse,cells |
| R5132 |
T17474 |
T17477 |
nsubjpass |
cells,maintained |
| R5133 |
T17475 |
T17474 |
compound |
R1,cells |
| R5134 |
T17476 |
T17474 |
compound |
ES,cells |
| R5135 |
T17478 |
T17479 |
punct |
[,49 |
| R5136 |
T17479 |
T17474 |
parataxis |
49,cells |
| R5137 |
T17480 |
T17479 |
punct |
],49 |
| R5138 |
T17481 |
T17477 |
auxpass |
were,maintained |
| R5139 |
T17482 |
T17477 |
prep |
on,maintained |
| R5140 |
T17483 |
T17484 |
npadvmod |
gelatin,coated |
| R5141 |
T17484 |
T17486 |
amod |
coated,plates |
| R5142 |
T17485 |
T17484 |
punct |
-,coated |
| R5143 |
T17486 |
T17482 |
pobj |
plates,on |
| R5144 |
T17487 |
T17477 |
prep |
with,maintained |
| R5145 |
T17488 |
T17489 |
compound |
feeder,layers |
| R5146 |
T17489 |
T17487 |
pobj |
layers,with |
| R5147 |
T17490 |
T17489 |
prep |
of,layers |
| R5148 |
T17491 |
T17492 |
nmod |
mouse,fibroblasts |
| R5149 |
T17492 |
T17490 |
pobj |
fibroblasts,of |
| R5150 |
T17493 |
T17492 |
amod |
embryonic,fibroblasts |
| R5151 |
T17494 |
T17477 |
prep |
at,maintained |
| R5152 |
T17495 |
T17496 |
nummod |
37,°C |
| R5153 |
T17496 |
T17494 |
pobj |
°C,at |
| R5154 |
T17497 |
T17477 |
prep |
under,maintained |
| R5155 |
T17498 |
T17499 |
nummod |
5,% |
| R5156 |
T17499 |
T17500 |
compound |
%,CO2 |
| R5157 |
T17500 |
T17497 |
pobj |
CO2,under |
| R5158 |
T17501 |
T17477 |
prep |
in,maintained |
| R5159 |
T17502 |
T17503 |
compound |
ES,medium |
| R5160 |
T17503 |
T17501 |
pobj |
medium,in |
| R5161 |
T17504 |
T17503 |
compound |
cell,medium |
| R5162 |
T17505 |
T17506 |
punct |
(,DMEM |
| R5163 |
T17506 |
T17477 |
parataxis |
DMEM,maintained |
| R5164 |
T17507 |
T17508 |
punct |
[,glucose |
| R5165 |
T17508 |
T17506 |
parataxis |
glucose,DMEM |
| R5166 |
T17509 |
T17508 |
amod |
high,glucose |
| R5167 |
T17510 |
T17506 |
punct |
],DMEM |
| R5168 |
T17511 |
T17506 |
acl |
supplemented,DMEM |
| R5169 |
T17512 |
T17511 |
prep |
with,supplemented |
| R5170 |
T17513 |
T17514 |
nummod |
15,% |
| R5171 |
T17514 |
T17515 |
nmod |
%,serum |
| R5172 |
T17515 |
T17512 |
pobj |
serum,with |
| R5173 |
T17516 |
T17515 |
amod |
fetal,serum |
| R5174 |
T17517 |
T17515 |
amod |
bovine,serum |
| R5175 |
T17518 |
T17515 |
punct |
", ",serum |
| R5176 |
T17519 |
T17520 |
nummod |
0.1,mM |
| R5177 |
T17520 |
T17521 |
nmod |
mM,acids |
| R5178 |
T17521 |
T17515 |
conj |
acids,serum |
| R5179 |
T17522 |
T17521 |
amod |
non-essential,acids |
| R5180 |
T17523 |
T17521 |
compound |
amino,acids |
| R5181 |
T17524 |
T17521 |
punct |
", ",acids |
| R5182 |
T17525 |
T17526 |
nummod |
2,mM |
| R5183 |
T17526 |
T17527 |
compound |
mM,glutamine |
| R5184 |
T17527 |
T17521 |
conj |
glutamine,acids |
| R5185 |
T17528 |
T17527 |
punct |
", ",glutamine |
| R5186 |
T17529 |
T17530 |
nummod |
1,mM |
| R5187 |
T17530 |
T17531 |
compound |
mM,pyruvate |
| R5188 |
T17531 |
T17527 |
conj |
pyruvate,glutamine |
| R5189 |
T17532 |
T17531 |
compound |
sodium,pyruvate |
| R5190 |
T17533 |
T17531 |
punct |
", ",pyruvate |
| R5191 |
T17534 |
T17535 |
nummod |
0.1,mM |
| R5192 |
T17535 |
T17536 |
nmod |
mM,mercaptoethanol |
| R5193 |
T17536 |
T17531 |
conj |
mercaptoethanol,pyruvate |
| R5194 |
T17537 |
T17536 |
nummod |
2,mercaptoethanol |
| R5195 |
T17538 |
T17536 |
punct |
-,mercaptoethanol |
| R5196 |
T17539 |
T17536 |
punct |
", ",mercaptoethanol |
| R5197 |
T17540 |
T17541 |
nummod |
100,U |
| R5198 |
T17541 |
T17542 |
nmod |
U,penicillin |
| R5199 |
T17542 |
T17536 |
conj |
penicillin,mercaptoethanol |
| R5200 |
T17543 |
T17544 |
punct |
/,ml |
| R5201 |
T17544 |
T17541 |
prep |
ml,U |
| R5202 |
T17545 |
T17542 |
punct |
", ",penicillin |
| R5203 |
T17546 |
T17547 |
nummod |
100,μg |
| R5204 |
T17547 |
T17548 |
nmod |
μg,streptomycin |
| R5205 |
T17548 |
T17542 |
conj |
streptomycin,penicillin |
| R5206 |
T17549 |
T17550 |
punct |
/,ml |
| R5207 |
T17550 |
T17547 |
prep |
ml,μg |
| R5208 |
T17551 |
T17548 |
punct |
", ",streptomycin |
| R5209 |
T17552 |
T17553 |
nummod |
25,μg |
| R5210 |
T17553 |
T17554 |
nmod |
μg,amphotericin |
| R5211 |
T17554 |
T17548 |
conj |
amphotericin,streptomycin |
| R5212 |
T17555 |
T17556 |
punct |
/,ml |
| R5213 |
T17556 |
T17553 |
prep |
ml,μg |
| R5214 |
T17557 |
T17554 |
punct |
", ",amphotericin |
| R5215 |
T17558 |
T17554 |
cc |
and,amphotericin |
| R5216 |
T17559 |
T17560 |
nummod |
"1,000",U |
| R5217 |
T17560 |
T17554 |
conj |
U,amphotericin |
| R5218 |
T17561 |
T17562 |
punct |
/,ml |
| R5219 |
T17562 |
T17560 |
prep |
ml,U |
| R5220 |
T17563 |
T17560 |
prep |
of,U |
| R5221 |
T17564 |
T17563 |
pobj |
ESGro,of |
| R5222 |
T17565 |
T17566 |
punct |
[,Chemicon |
| R5223 |
T17566 |
T17564 |
parataxis |
Chemicon,ESGro |
| R5224 |
T17567 |
T17566 |
punct |
],Chemicon |
| R5225 |
T17568 |
T17477 |
punct |
),maintained |
| R5226 |
T17569 |
T17477 |
punct |
.,maintained |
| R5227 |
T17571 |
T17572 |
amod |
Fetal,serum |
| R5228 |
T17572 |
T17574 |
nsubjpass |
serum,tested |
| R5229 |
T17573 |
T17572 |
amod |
bovine,serum |
| R5230 |
T17575 |
T17572 |
prep |
from,serum |
| R5231 |
T17576 |
T17577 |
compound |
HyClone,Inc |
| R5232 |
T17577 |
T17575 |
pobj |
Inc,from |
| R5233 |
T17578 |
T17577 |
compound |
Laboratories,Inc |
| R5234 |
T17579 |
T17580 |
punct |
(,Logan |
| R5235 |
T17580 |
T17577 |
parataxis |
Logan,Inc |
| R5236 |
T17581 |
T17580 |
punct |
", ",Logan |
| R5237 |
T17582 |
T17580 |
npadvmod |
Utah,Logan |
| R5238 |
T17583 |
T17580 |
punct |
),Logan |
| R5239 |
T17584 |
T17574 |
auxpass |
was,tested |
| R5240 |
T17585 |
T17574 |
prep |
for,tested |
| R5241 |
T17586 |
T17587 |
poss |
its,ability |
| R5242 |
T17587 |
T17585 |
pobj |
ability,for |
| R5243 |
T17588 |
T17589 |
aux |
to,support |
| R5244 |
T17589 |
T17587 |
acl |
support,ability |
| R5245 |
T17590 |
T17591 |
compound |
ES,cell |
| R5246 |
T17591 |
T17592 |
compound |
cell,growth |
| R5247 |
T17592 |
T17589 |
dobj |
growth,support |
| R5248 |
T17593 |
T17574 |
punct |
.,tested |
| R5249 |
T17595 |
T17596 |
nsubj |
Gelatin,was |
| R5250 |
T17597 |
T17596 |
prep |
from,was |
| R5251 |
T17598 |
T17599 |
compound |
Sigma,Aldrich |
| R5252 |
T17599 |
T17601 |
compound |
Aldrich,Canada |
| R5253 |
T17600 |
T17599 |
punct |
-,Aldrich |
| R5254 |
T17601 |
T17597 |
pobj |
Canada,from |
| R5255 |
T17602 |
T17603 |
punct |
(,Oakville |
| R5256 |
T17603 |
T17596 |
parataxis |
Oakville,was |
| R5257 |
T17604 |
T17603 |
punct |
", ",Oakville |
| R5258 |
T17605 |
T17603 |
npadvmod |
Ontario,Oakville |
| R5259 |
T17606 |
T17603 |
punct |
),Oakville |
| R5260 |
T17607 |
T17596 |
punct |
.,was |
| R5261 |
T17609 |
T17610 |
mark |
Unless,specified |
| R5262 |
T17610 |
T17612 |
advcl |
specified,were |
| R5263 |
T17611 |
T17610 |
advmod |
otherwise,specified |
| R5264 |
T17613 |
T17612 |
punct |
", ",were |
| R5265 |
T17614 |
T17615 |
det |
all,reagents |
| R5266 |
T17615 |
T17612 |
nsubj |
reagents,were |
| R5267 |
T17616 |
T17615 |
amod |
other,reagents |
| R5268 |
T17617 |
T17618 |
compound |
tissue,culture |
| R5269 |
T17618 |
T17615 |
compound |
culture,reagents |
| R5270 |
T17619 |
T17612 |
prep |
from,were |
| R5271 |
T17620 |
T17621 |
compound |
Gibco,BRL |
| R5272 |
T17621 |
T17619 |
pobj |
BRL,from |
| R5273 |
T17622 |
T17621 |
punct |
-,BRL |
| R5274 |
T17623 |
T17612 |
punct |
.,were |
| R5275 |
T17625 |
T17626 |
det |
The,construct |
| R5276 |
T17626 |
T17628 |
nsubjpass |
construct,linearized |
| R5277 |
T17627 |
T17626 |
compound |
targeting,construct |
| R5278 |
T17629 |
T17628 |
auxpass |
was,linearized |
| R5279 |
T17630 |
T17628 |
agent |
by,linearized |
| R5280 |
T17631 |
T17632 |
compound |
NotI,digestion |
| R5281 |
T17632 |
T17630 |
pobj |
digestion,by |
| R5282 |
T17633 |
T17628 |
cc |
and,linearized |
| R5283 |
T17634 |
T17628 |
conj |
electroporated,linearized |
| R5284 |
T17635 |
T17634 |
prep |
into,electroporated |
| R5285 |
T17636 |
T17637 |
compound |
R1,cells |
| R5286 |
T17637 |
T17635 |
pobj |
cells,into |
| R5287 |
T17638 |
T17637 |
compound |
ES,cells |
| R5288 |
T17639 |
T17628 |
punct |
.,linearized |
| R5289 |
T17641 |
T17642 |
nsubjpass |
Cells,plated |
| R5290 |
T17643 |
T17642 |
auxpass |
were,plated |
| R5291 |
T17644 |
T17642 |
prep |
without,plated |
| R5292 |
T17645 |
T17646 |
compound |
feeder,layers |
| R5293 |
T17646 |
T17644 |
pobj |
layers,without |
| R5294 |
T17647 |
T17642 |
prep |
on,plated |
| R5295 |
T17648 |
T17649 |
npadvmod |
gelatin,coated |
| R5296 |
T17649 |
T17651 |
amod |
coated,plates |
| R5297 |
T17650 |
T17649 |
punct |
-,coated |
| R5298 |
T17651 |
T17647 |
pobj |
plates,on |
| R5299 |
T17652 |
T17642 |
cc |
and,plated |
| R5300 |
T17653 |
T17654 |
amod |
transformed,clones |
| R5301 |
T17654 |
T17655 |
nsubjpass |
clones,selected |
| R5302 |
T17655 |
T17642 |
conj |
selected,plated |
| R5303 |
T17656 |
T17655 |
auxpass |
were,selected |
| R5304 |
T17657 |
T17655 |
prep |
in,selected |
| R5305 |
T17658 |
T17659 |
det |
the,presence |
| R5306 |
T17659 |
T17657 |
pobj |
presence,in |
| R5307 |
T17660 |
T17659 |
prep |
of,presence |
| R5308 |
T17661 |
T17662 |
nummod |
200,μg |
| R5309 |
T17662 |
T17663 |
nmod |
μg,G418 |
| R5310 |
T17663 |
T17660 |
pobj |
G418,of |
| R5311 |
T17664 |
T17665 |
punct |
/,ml |
| R5312 |
T17665 |
T17662 |
prep |
ml,μg |
| R5313 |
T17666 |
T17667 |
punct |
(,BRL |
| R5314 |
T17667 |
T17663 |
parataxis |
BRL,G418 |
| R5315 |
T17668 |
T17667 |
compound |
Gibco,BRL |
| R5316 |
T17669 |
T17667 |
punct |
-,BRL |
| R5317 |
T17670 |
T17667 |
punct |
),BRL |
| R5318 |
T17671 |
T17663 |
cc |
and,G418 |
| R5319 |
T17672 |
T17673 |
nummod |
2,μM |
| R5320 |
T17673 |
T17674 |
compound |
μM,ganciclovir |
| R5321 |
T17674 |
T17663 |
conj |
ganciclovir,G418 |
| R5322 |
T17675 |
T17676 |
punct |
(,Syntex |
| R5323 |
T17676 |
T17674 |
parataxis |
Syntex,ganciclovir |
| R5324 |
T17677 |
T17676 |
punct |
", ",Syntex |
| R5325 |
T17678 |
T17676 |
amod |
Inc.,Syntex |
| R5326 |
T17679 |
T17676 |
punct |
),Syntex |
| R5327 |
T17680 |
T17655 |
prep |
for,selected |
| R5328 |
T17681 |
T17682 |
nummod |
eight,days |
| R5329 |
T17682 |
T17680 |
pobj |
days,for |
| R5330 |
T17683 |
T17655 |
punct |
.,selected |
| R5331 |
T17685 |
T17686 |
npadvmod |
Drug,resistant |
| R5332 |
T17686 |
T17688 |
amod |
resistant,clones |
| R5333 |
T17687 |
T17686 |
punct |
-,resistant |
| R5334 |
T17688 |
T17689 |
nsubjpass |
clones,picked |
| R5335 |
T17690 |
T17689 |
auxpass |
were,picked |
| R5336 |
T17691 |
T17689 |
punct |
", ",picked |
| R5337 |
T17692 |
T17689 |
conj |
expanded,picked |
| R5338 |
T17693 |
T17692 |
prep |
on,expanded |
| R5339 |
T17694 |
T17695 |
npadvmod |
gelatin,coated |
| R5340 |
T17695 |
T17697 |
amod |
coated,plates |
| R5341 |
T17696 |
T17695 |
punct |
-,coated |
| R5342 |
T17697 |
T17693 |
pobj |
plates,on |
| R5343 |
T17698 |
T17692 |
punct |
", ",expanded |
| R5344 |
T17699 |
T17692 |
cc |
and,expanded |
| R5345 |
T17700 |
T17692 |
conj |
genotyped,expanded |
| R5346 |
T17701 |
T17700 |
prep |
by,genotyped |
| R5347 |
T17702 |
T17703 |
compound |
Southern,blotting |
| R5348 |
T17703 |
T17701 |
pobj |
blotting,by |
| R5349 |
T17704 |
T17703 |
cc |
and,blotting |
| R5350 |
T17705 |
T17706 |
compound |
PCR,analysis |
| R5351 |
T17706 |
T17703 |
conj |
analysis,blotting |
| R5352 |
T17707 |
T17708 |
punct |
(,see |
| R5353 |
T17708 |
T17700 |
parataxis |
see,genotyped |
| R5354 |
T17709 |
T17708 |
advmod |
below,see |
| R5355 |
T17710 |
T17708 |
punct |
),see |
| R5356 |
T17711 |
T17689 |
punct |
.,picked |
