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PMC:1382200 / 31664-32752 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T23423 0-9 NN denotes Targeting
T23424 10-18 NN denotes strategy
T23425 19-22 IN denotes for
T23426 23-33 NN denotes disruption
T23427 34-36 IN denotes of
T23428 37-40 DT denotes the
T23430 41-47 JJ denotes murine
T23431 48-53 NN denotes Capn2
T23429 54-58 NN denotes gene
T23432 58-59 . denotes .
T23433 59-166 sentence denotes The murine Capn2 gene, encoding the m-80 k subunit, was disrupted in ES cells by homologous recombination.
T23434 60-63 DT denotes The
T23436 64-70 JJ denotes murine
T23437 71-76 NN denotes Capn2
T23435 77-81 NN denotes gene
T23439 81-83 , denotes ,
T23440 83-91 VBG denotes encoding
T23441 92-95 DT denotes the
T23443 96-97 NN denotes m
T23445 97-98 HYPH denotes -
T23446 98-100 CD denotes 80
T23444 101-102 NN denotes k
T23442 103-110 NN denotes subunit
T23447 110-112 , denotes ,
T23448 112-115 VBD denotes was
T23438 116-125 VBN denotes disrupted
T23449 126-128 IN denotes in
T23450 129-131 NN denotes ES
T23451 132-137 NNS denotes cells
T23452 138-140 IN denotes by
T23453 141-151 JJ denotes homologous
T23454 152-165 NN denotes recombination
T23455 165-166 . denotes .
T23456 166-288 sentence denotes The structures of the wild-type allele (top), the targeting vector (middle), and the mutant allele (bottom) are depicted.
T23457 167-170 DT denotes The
T23458 171-181 NNS denotes structures
T23460 182-184 IN denotes of
T23461 185-188 DT denotes the
T23463 189-193 JJ denotes wild
T23465 193-194 HYPH denotes -
T23464 194-198 NN denotes type
T23462 199-205 NN denotes allele
T23466 206-207 -LRB- denotes (
T23467 207-210 JJ denotes top
T23468 210-211 -RRB- denotes )
T23469 211-213 , denotes ,
T23470 213-216 DT denotes the
T23472 217-226 NN denotes targeting
T23471 227-233 NN denotes vector
T23473 234-235 -LRB- denotes (
T23474 235-241 JJ denotes middle
T23475 241-242 -RRB- denotes )
T23476 242-244 , denotes ,
T23477 244-247 CC denotes and
T23478 248-251 DT denotes the
T23480 252-258 NN denotes mutant
T23479 259-265 NN denotes allele
T23481 266-267 -LRB- denotes (
T23482 267-273 JJ denotes bottom
T23483 273-274 -RRB- denotes )
T23484 275-278 VBP denotes are
T23459 279-287 VBN denotes depicted
T23485 287-288 . denotes .
T23486 288-634 sentence denotes In the mutant allele, a PGK-Neo cassette replaces a 0.8-kb genomic fragment containing exon 7 (grey rectangle) which encodes the active site asparagine residue (Asn286) In the targeting vector, the PGK-Neo cassette is flanked by 2.7-kb of Capn2 homologous sequence in the upstream (short) arm and 7.9-kb of homology in the downstream (long) arm.
T23487 289-291 IN denotes In
T23489 292-295 DT denotes the
T23491 296-302 NN denotes mutant
T23490 303-309 NN denotes allele
T23492 309-311 , denotes ,
T23493 311-312 DT denotes a
T23495 313-316 NN denotes PGK
T23497 316-317 HYPH denotes -
T23496 317-320 NN denotes Neo
T23494 321-329 NN denotes cassette
T23488 330-338 VBZ denotes replaces
T23499 339-340 DT denotes a
T23501 341-344 CD denotes 0.8
T23503 344-345 HYPH denotes -
T23502 345-347 NN denotes kb
T23504 348-355 JJ denotes genomic
T23500 356-364 NN denotes fragment
T23505 365-375 VBG denotes containing
T23506 376-380 NN denotes exon
T23507 381-382 CD denotes 7
T23508 383-384 -LRB- denotes (
T23510 384-388 JJ denotes grey
T23509 389-398 NN denotes rectangle
T23511 398-399 -RRB- denotes )
T23512 400-405 WDT denotes which
T23513 406-413 VBZ denotes encodes
T23514 414-417 DT denotes the
T23516 418-424 JJ denotes active
T23517 425-429 NN denotes site
T23518 430-440 NN denotes asparagine
T23515 441-448 NN denotes residue
T23519 449-450 -LRB- denotes (
T23520 450-456 NN denotes Asn286
T23521 456-457 -RRB- denotes )
T23522 458-460 IN denotes In
T23523 461-464 DT denotes the
T23525 465-474 NN denotes targeting
T23524 475-481 NN denotes vector
T23526 481-483 , denotes ,
T23527 483-486 DT denotes the
T23529 487-490 NN denotes PGK
T23531 490-491 HYPH denotes -
T23530 491-494 NN denotes Neo
T23528 495-503 NN denotes cassette
T23532 504-506 VBZ denotes is
T23498 507-514 VBN denotes flanked
T23533 515-517 IN denotes by
T23534 518-521 CD denotes 2.7
T23536 521-522 HYPH denotes -
T23535 522-524 NN denotes kb
T23537 525-527 IN denotes of
T23538 528-533 NN denotes Capn2
T23540 534-544 JJ denotes homologous
T23539 545-553 NN denotes sequence
T23541 554-556 IN denotes in
T23542 557-560 DT denotes the
T23544 561-569 JJ denotes upstream
T23545 570-571 -LRB- denotes (
T23546 571-576 JJ denotes short
T23547 576-577 -RRB- denotes )
T23543 578-581 NN denotes arm
T23548 582-585 CC denotes and
T23549 586-589 CD denotes 7.9
T23551 589-590 HYPH denotes -
T23550 590-592 NN denotes kb
T23552 593-595 IN denotes of
T23553 596-604 NN denotes homology
T23554 605-607 IN denotes in
T23555 608-611 DT denotes the
T23557 612-622 JJ denotes downstream
T23558 623-624 -LRB- denotes (
T23559 624-628 JJ denotes long
T23560 628-629 -RRB- denotes )
T23556 630-633 NN denotes arm
T23561 633-634 . denotes .
T23562 634-797 sentence denotes A probe located immediately outside of the short arm detects a 3.5-kb BamHI fragment from the wild-type allele and a 5.3-kb BamHI fragment from the mutant allele.
T23563 635-636 DT denotes A
T23564 637-642 NN denotes probe
T23566 643-650 VBN denotes located
T23567 651-662 RB denotes immediately
T23568 663-670 JJ denotes outside
T23569 671-673 IN denotes of
T23570 674-677 DT denotes the
T23572 678-683 JJ denotes short
T23571 684-687 NN denotes arm
T23565 688-695 VBZ denotes detects
T23573 696-697 DT denotes a
T23575 698-701 CD denotes 3.5
T23577 701-702 HYPH denotes -
T23576 702-704 NN denotes kb
T23578 705-710 NN denotes BamHI
T23574 711-719 NN denotes fragment
T23579 720-724 IN denotes from
T23580 725-728 DT denotes the
T23582 729-733 JJ denotes wild
T23584 733-734 HYPH denotes -
T23583 734-738 NN denotes type
T23581 739-745 NN denotes allele
T23585 746-749 CC denotes and
T23586 750-751 DT denotes a
T23588 752-755 CD denotes 5.3
T23590 755-756 HYPH denotes -
T23589 756-758 NN denotes kb
T23591 759-764 NN denotes BamHI
T23587 765-773 NN denotes fragment
T23592 774-778 IN denotes from
T23593 779-782 DT denotes the
T23595 783-789 NN denotes mutant
T23594 790-796 NN denotes allele
T23596 796-797 . denotes .
T23597 797-914 sentence denotes Exons are depicted as open vertical rectangles except for exon 7 which is represented by a solid vertical rectangle.
T23598 798-803 NNS denotes Exons
T23600 804-807 VBP denotes are
T23599 808-816 VBN denotes depicted
T23601 817-819 IN denotes as
T23602 820-824 JJ denotes open
T23604 825-833 JJ denotes vertical
T23603 834-844 NNS denotes rectangles
T23605 845-851 IN denotes except
T23606 852-855 IN denotes for
T23607 856-860 NN denotes exon
T23608 861-862 CD denotes 7
T23609 863-868 WDT denotes which
T23611 869-871 VBZ denotes is
T23610 872-883 VBN denotes represented
T23612 884-886 IN denotes by
T23613 887-888 DT denotes a
T23615 889-894 JJ denotes solid
T23616 895-903 JJ denotes vertical
T23614 904-913 NN denotes rectangle
T23617 913-914 . denotes .
T23618 914-1088 sentence denotes The probe used in most Southern blot analyses is shown as a solid, horizontal rectangle, while triangles mark the positions of PCR primers also used for genotyping purposes.
T23619 915-918 DT denotes The
T23620 919-924 NN denotes probe
T23622 925-929 VBN denotes used
T23623 930-932 IN denotes in
T23624 933-937 JJS denotes most
T23626 938-946 NNP denotes Southern
T23627 947-951 NN denotes blot
T23625 952-960 NNS denotes analyses
T23628 961-963 VBZ denotes is
T23621 964-969 VBN denotes shown
T23629 970-972 IN denotes as
T23630 973-974 DT denotes a
T23632 975-980 JJ denotes solid
T23633 980-982 , denotes ,
T23634 982-992 JJ denotes horizontal
T23631 993-1002 NN denotes rectangle
T23635 1002-1004 , denotes ,
T23636 1004-1009 IN denotes while
T23638 1010-1019 NNS denotes triangles
T23637 1020-1024 VBP denotes mark
T23639 1025-1028 DT denotes the
T23640 1029-1038 NNS denotes positions
T23641 1039-1041 IN denotes of
T23642 1042-1045 NN denotes PCR
T23643 1046-1053 NNS denotes primers
T23644 1054-1058 RB denotes also
T23645 1059-1063 VBN denotes used
T23646 1064-1067 IN denotes for
T23647 1068-1078 NN denotes genotyping
T23648 1079-1087 NNS denotes purposes
T23649 1087-1088 . denotes .
R6939 T23423 T23424 compound Targeting,strategy
R6940 T23425 T23424 prep for,strategy
R6941 T23426 T23425 pobj disruption,for
R6942 T23427 T23426 prep of,disruption
R6943 T23428 T23429 det the,gene
R6944 T23429 T23427 pobj gene,of
R6945 T23430 T23429 amod murine,gene
R6946 T23431 T23429 compound Capn2,gene
R6947 T23432 T23424 punct .,strategy
R6948 T23434 T23435 det The,gene
R6949 T23435 T23438 nsubjpass gene,disrupted
R6950 T23436 T23435 amod murine,gene
R6951 T23437 T23435 compound Capn2,gene
R6952 T23439 T23435 punct ", ",gene
R6953 T23440 T23435 acl encoding,gene
R6954 T23441 T23442 det the,subunit
R6955 T23442 T23440 dobj subunit,encoding
R6956 T23443 T23444 nmod m,k
R6957 T23444 T23442 compound k,subunit
R6958 T23445 T23444 punct -,k
R6959 T23446 T23444 nummod 80,k
R6960 T23447 T23438 punct ", ",disrupted
R6961 T23448 T23438 auxpass was,disrupted
R6962 T23449 T23438 prep in,disrupted
R6963 T23450 T23451 compound ES,cells
R6964 T23451 T23449 pobj cells,in
R6965 T23452 T23438 agent by,disrupted
R6966 T23453 T23454 amod homologous,recombination
R6967 T23454 T23452 pobj recombination,by
R6968 T23455 T23438 punct .,disrupted
R6969 T23457 T23458 det The,structures
R6970 T23458 T23459 nsubjpass structures,depicted
R6971 T23460 T23458 prep of,structures
R6972 T23461 T23462 det the,allele
R6973 T23462 T23460 pobj allele,of
R6974 T23463 T23464 amod wild,type
R6975 T23464 T23462 compound type,allele
R6976 T23465 T23464 punct -,type
R6977 T23466 T23467 punct (,top
R6978 T23467 T23462 parataxis top,allele
R6979 T23468 T23467 punct ),top
R6980 T23469 T23462 punct ", ",allele
R6981 T23470 T23471 det the,vector
R6982 T23471 T23462 conj vector,allele
R6983 T23472 T23471 compound targeting,vector
R6984 T23473 T23474 punct (,middle
R6985 T23474 T23471 parataxis middle,vector
R6986 T23475 T23474 punct ),middle
R6987 T23476 T23471 punct ", ",vector
R6988 T23477 T23471 cc and,vector
R6989 T23478 T23479 det the,allele
R6990 T23479 T23471 conj allele,vector
R6991 T23480 T23479 compound mutant,allele
R6992 T23481 T23482 punct (,bottom
R6993 T23482 T23479 parataxis bottom,allele
R6994 T23483 T23482 punct ),bottom
R6995 T23484 T23459 auxpass are,depicted
R6996 T23485 T23459 punct .,depicted
R6997 T23487 T23488 prep In,replaces
R6998 T23488 T23498 ccomp replaces,flanked
R6999 T23489 T23490 det the,allele
R7000 T23490 T23487 pobj allele,In
R7001 T23491 T23490 compound mutant,allele
R7002 T23492 T23488 punct ", ",replaces
R7003 T23493 T23494 det a,cassette
R7004 T23494 T23488 nsubj cassette,replaces
R7005 T23495 T23496 compound PGK,Neo
R7006 T23496 T23494 compound Neo,cassette
R7007 T23497 T23496 punct -,Neo
R7008 T23499 T23500 det a,fragment
R7009 T23500 T23488 dobj fragment,replaces
R7010 T23501 T23502 nummod 0.8,kb
R7011 T23502 T23500 nmod kb,fragment
R7012 T23503 T23502 punct -,kb
R7013 T23504 T23500 amod genomic,fragment
R7014 T23505 T23500 acl containing,fragment
R7015 T23506 T23505 dobj exon,containing
R7016 T23507 T23506 nummod 7,exon
R7017 T23508 T23509 punct (,rectangle
R7018 T23509 T23506 parataxis rectangle,exon
R7019 T23510 T23509 amod grey,rectangle
R7020 T23511 T23509 punct ),rectangle
R7021 T23512 T23513 dep which,encodes
R7022 T23513 T23500 relcl encodes,fragment
R7023 T23514 T23515 det the,residue
R7024 T23515 T23513 dobj residue,encodes
R7025 T23516 T23515 amod active,residue
R7026 T23517 T23515 compound site,residue
R7027 T23518 T23515 compound asparagine,residue
R7028 T23519 T23515 punct (,residue
R7029 T23520 T23515 appos Asn286,residue
R7030 T23521 T23498 punct ),flanked
R7031 T23522 T23498 prep In,flanked
R7032 T23523 T23524 det the,vector
R7033 T23524 T23522 pobj vector,In
R7034 T23525 T23524 compound targeting,vector
R7035 T23526 T23498 punct ", ",flanked
R7036 T23527 T23528 det the,cassette
R7037 T23528 T23498 nsubjpass cassette,flanked
R7038 T23529 T23530 compound PGK,Neo
R7039 T23530 T23528 compound Neo,cassette
R7040 T23531 T23530 punct -,Neo
R7041 T23532 T23498 auxpass is,flanked
R7042 T23533 T23498 agent by,flanked
R7043 T23534 T23535 nummod 2.7,kb
R7044 T23535 T23533 pobj kb,by
R7045 T23536 T23535 punct -,kb
R7046 T23537 T23535 prep of,kb
R7047 T23538 T23539 nmod Capn2,sequence
R7048 T23539 T23537 pobj sequence,of
R7049 T23540 T23539 amod homologous,sequence
R7050 T23541 T23498 prep in,flanked
R7051 T23542 T23543 det the,arm
R7052 T23543 T23541 pobj arm,in
R7053 T23544 T23543 amod upstream,arm
R7054 T23545 T23543 punct (,arm
R7055 T23546 T23543 amod short,arm
R7056 T23547 T23543 punct ),arm
R7057 T23548 T23498 cc and,flanked
R7058 T23549 T23550 nummod 7.9,kb
R7059 T23550 T23498 conj kb,flanked
R7060 T23551 T23550 punct -,kb
R7061 T23552 T23550 prep of,kb
R7062 T23553 T23552 pobj homology,of
R7063 T23554 T23550 prep in,kb
R7064 T23555 T23556 det the,arm
R7065 T23556 T23554 pobj arm,in
R7066 T23557 T23556 amod downstream,arm
R7067 T23558 T23556 punct (,arm
R7068 T23559 T23556 amod long,arm
R7069 T23560 T23556 punct ),arm
R7070 T23561 T23498 punct .,flanked
R7071 T23563 T23564 det A,probe
R7072 T23564 T23565 nsubj probe,detects
R7073 T23566 T23564 acl located,probe
R7074 T23567 T23568 advmod immediately,outside
R7075 T23568 T23566 advmod outside,located
R7076 T23569 T23568 prep of,outside
R7077 T23570 T23571 det the,arm
R7078 T23571 T23569 pobj arm,of
R7079 T23572 T23571 amod short,arm
R7080 T23573 T23574 det a,fragment
R7081 T23574 T23565 dobj fragment,detects
R7082 T23575 T23576 nummod 3.5,kb
R7083 T23576 T23574 compound kb,fragment
R7084 T23577 T23576 punct -,kb
R7085 T23578 T23574 compound BamHI,fragment
R7086 T23579 T23565 prep from,detects
R7087 T23580 T23581 det the,allele
R7088 T23581 T23579 pobj allele,from
R7089 T23582 T23583 amod wild,type
R7090 T23583 T23581 compound type,allele
R7091 T23584 T23583 punct -,type
R7092 T23585 T23565 cc and,detects
R7093 T23586 T23587 det a,fragment
R7094 T23587 T23565 conj fragment,detects
R7095 T23588 T23589 nummod 5.3,kb
R7096 T23589 T23587 compound kb,fragment
R7097 T23590 T23589 punct -,kb
R7098 T23591 T23587 compound BamHI,fragment
R7099 T23592 T23587 prep from,fragment
R7100 T23593 T23594 det the,allele
R7101 T23594 T23592 pobj allele,from
R7102 T23595 T23594 compound mutant,allele
R7103 T23596 T23565 punct .,detects
R7104 T23598 T23599 nsubjpass Exons,depicted
R7105 T23600 T23599 auxpass are,depicted
R7106 T23601 T23599 prep as,depicted
R7107 T23602 T23603 amod open,rectangles
R7108 T23603 T23601 pobj rectangles,as
R7109 T23604 T23603 amod vertical,rectangles
R7110 T23605 T23599 prep except,depicted
R7111 T23606 T23605 prep for,except
R7112 T23607 T23606 pobj exon,for
R7113 T23608 T23607 nummod 7,exon
R7114 T23609 T23610 dep which,represented
R7115 T23610 T23607 relcl represented,exon
R7116 T23611 T23610 auxpass is,represented
R7117 T23612 T23610 agent by,represented
R7118 T23613 T23614 det a,rectangle
R7119 T23614 T23612 pobj rectangle,by
R7120 T23615 T23614 amod solid,rectangle
R7121 T23616 T23614 amod vertical,rectangle
R7122 T23617 T23599 punct .,depicted
R7123 T23619 T23620 det The,probe
R7124 T23620 T23621 nsubjpass probe,shown
R7125 T23622 T23620 acl used,probe
R7126 T23623 T23622 prep in,used
R7127 T23624 T23625 amod most,analyses
R7128 T23625 T23623 pobj analyses,in
R7129 T23626 T23627 compound Southern,blot
R7130 T23627 T23625 compound blot,analyses
R7131 T23628 T23621 auxpass is,shown
R7132 T23629 T23621 prep as,shown
R7133 T23630 T23631 det a,rectangle
R7134 T23631 T23629 pobj rectangle,as
R7135 T23632 T23631 amod solid,rectangle
R7136 T23633 T23631 punct ", ",rectangle
R7137 T23634 T23631 amod horizontal,rectangle
R7138 T23635 T23621 punct ", ",shown
R7139 T23636 T23637 mark while,mark
R7140 T23637 T23621 advcl mark,shown
R7141 T23638 T23637 nsubj triangles,mark
R7142 T23639 T23640 det the,positions
R7143 T23640 T23637 dobj positions,mark
R7144 T23641 T23640 prep of,positions
R7145 T23642 T23643 compound PCR,primers
R7146 T23643 T23641 pobj primers,of
R7147 T23644 T23645 advmod also,used
R7148 T23645 T23643 acl used,primers
R7149 T23646 T23645 prep for,used
R7150 T23647 T23648 compound genotyping,purposes
R7151 T23648 T23646 pobj purposes,for
R7152 T23649 T23621 punct .,shown