craft-sa-dev Home  

PMC:1382200 / 30687-31653 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function

Id Subject Object Predicate Lexical cue
T16716 0-1 DT denotes A
T16718 2-11 NN denotes targeting
T16717 12-21 NN denotes construct
T16720 22-25 VBD denotes was
T16719 26-34 VBN denotes designed
T16721 35-37 TO denotes to
T16722 38-45 VB denotes replace
T16723 46-47 DT denotes a
T16725 48-51 CD denotes 785
T16726 52-54 NN denotes bp
T16727 55-60 NN denotes BamHI
T16729 60-61 HYPH denotes -
T16728 61-68 NN denotes HindIII
T16724 69-77 NN denotes fragment
T16730 77-79 , denotes ,
T16731 79-89 VBG denotes containing
T16732 90-94 NN denotes exon
T16733 95-96 CD denotes 7
T16734 97-99 IN denotes of
T16735 100-103 DT denotes the
T16737 104-109 NN denotes Capn2
T16736 110-114 NN denotes gene
T16738 114-116 , denotes ,
T16739 116-120 IN denotes with
T16740 121-124 DT denotes the
T16742 125-128 NN denotes PGK
T16744 128-129 HYPH denotes -
T16743 129-132 NN denotes Neo
T16741 133-141 NN denotes cassette
T16745 142-143 -LRB- denotes (
T16746 143-149 NN denotes Figure
T16747 150-151 CD denotes 1
T16748 151-152 -RRB- denotes )
T16749 152-153 . denotes .
T16750 153-265 sentence denotes Exon 7 encodes 24 amino acids in the active-site region, including Asn286, one of the catalytic triad residues.
T16751 154-158 NN denotes Exon
T16753 159-160 CD denotes 7
T16752 161-168 VBZ denotes encodes
T16754 169-171 CD denotes 24
T16756 172-177 NN denotes amino
T16755 178-183 NNS denotes acids
T16757 184-186 IN denotes in
T16758 187-190 DT denotes the
T16760 191-197 JJ denotes active
T16762 197-198 HYPH denotes -
T16761 198-202 NN denotes site
T16759 203-209 NN denotes region
T16763 209-211 , denotes ,
T16764 211-220 VBG denotes including
T16765 221-227 NN denotes Asn286
T16766 227-229 , denotes ,
T16767 229-232 CD denotes one
T16768 233-235 IN denotes of
T16769 236-239 DT denotes the
T16771 240-249 JJ denotes catalytic
T16772 250-255 NN denotes triad
T16770 256-264 NNS denotes residues
T16773 264-265 . denotes .
T16774 265-471 sentence denotes The short (upstream) arm of the targeting construct was provided by a 2.7-kb HindIII-BamHI fragment, containing exons 5 and 6, which was inserted into the pPNT vector upstream of the PGK-Neo cassette [48].
T16775 266-269 DT denotes The
T16777 270-275 JJ denotes short
T16778 276-277 -LRB- denotes (
T16779 277-285 JJ denotes upstream
T16780 285-286 -RRB- denotes )
T16776 287-290 NN denotes arm
T16782 291-293 IN denotes of
T16783 294-297 DT denotes the
T16785 298-307 NN denotes targeting
T16784 308-317 NN denotes construct
T16786 318-321 VBD denotes was
T16781 322-330 VBN denotes provided
T16787 331-333 IN denotes by
T16788 334-335 DT denotes a
T16790 336-339 CD denotes 2.7
T16792 339-340 HYPH denotes -
T16791 340-342 NN denotes kb
T16793 343-350 NN denotes HindIII
T16795 350-351 HYPH denotes -
T16794 351-356 NN denotes BamHI
T16789 357-365 NN denotes fragment
T16796 365-367 , denotes ,
T16797 367-377 VBG denotes containing
T16798 378-383 NNS denotes exons
T16799 384-385 CD denotes 5
T16800 386-389 CC denotes and
T16801 390-391 CD denotes 6
T16802 391-393 , denotes ,
T16803 393-398 WDT denotes which
T16805 399-402 VBD denotes was
T16804 403-411 VBN denotes inserted
T16806 412-416 IN denotes into
T16807 417-420 DT denotes the
T16809 421-425 NN denotes pPNT
T16808 426-432 NN denotes vector
T16810 433-441 RB denotes upstream
T16811 442-444 IN denotes of
T16812 445-448 DT denotes the
T16814 449-452 NN denotes PGK
T16816 452-453 HYPH denotes -
T16815 453-456 NN denotes Neo
T16813 457-465 NN denotes cassette
T16817 466-467 -LRB- denotes [
T16818 467-469 CD denotes 48
T16819 469-470 -RRB- denotes ]
T16820 470-471 . denotes .
T16821 471-548 sentence denotes During cloning, the BamHI site at the 3' end of the short arm was abolished.
T16822 472-478 IN denotes During
T16824 479-486 NN denotes cloning
T16825 486-488 , denotes ,
T16826 488-491 DT denotes the
T16828 492-497 NN denotes BamHI
T16827 498-502 NN denotes site
T16829 503-505 IN denotes at
T16830 506-509 DT denotes the
T16832 510-511 CD denotes 3
T16833 511-512 SYM denotes '
T16831 513-516 NN denotes end
T16834 517-519 IN denotes of
T16835 520-523 DT denotes the
T16837 524-529 JJ denotes short
T16836 530-533 NN denotes arm
T16838 534-537 VBD denotes was
T16823 538-547 VBN denotes abolished
T16839 547-548 . denotes .
T16840 548-778 sentence denotes The loss of this BamHI site in the mutant allele, coupled with the introduction of a new BamHI site at the 3' end of the PGK-Neo cassette, provided a basis for distinguishing the wild-type and mutant alleles by Southern blotting.
T16841 549-552 DT denotes The
T16842 553-557 NN denotes loss
T16844 558-560 IN denotes of
T16845 561-565 DT denotes this
T16847 566-571 NN denotes BamHI
T16846 572-576 NN denotes site
T16848 577-579 IN denotes in
T16849 580-583 DT denotes the
T16851 584-590 NN denotes mutant
T16850 591-597 NN denotes allele
T16852 597-599 , denotes ,
T16853 599-606 VBN denotes coupled
T16854 607-611 IN denotes with
T16855 612-615 DT denotes the
T16856 616-628 NN denotes introduction
T16857 629-631 IN denotes of
T16858 632-633 DT denotes a
T16860 634-637 JJ denotes new
T16861 638-643 NN denotes BamHI
T16859 644-648 NN denotes site
T16862 649-651 IN denotes at
T16863 652-655 DT denotes the
T16865 656-657 CD denotes 3
T16866 657-658 SYM denotes '
T16864 659-662 NN denotes end
T16867 663-665 IN denotes of
T16868 666-669 DT denotes the
T16870 670-673 NN denotes PGK
T16872 673-674 HYPH denotes -
T16871 674-677 NN denotes Neo
T16869 678-686 NN denotes cassette
T16873 686-688 , denotes ,
T16843 688-696 VBN denotes provided
T16874 697-698 DT denotes a
T16875 699-704 NN denotes basis
T16876 705-708 IN denotes for
T16877 709-723 VBG denotes distinguishing
T16878 724-727 DT denotes the
T16880 728-732 JJ denotes wild
T16882 732-733 HYPH denotes -
T16881 733-737 NN denotes type
T16883 738-741 CC denotes and
T16884 742-748 NN denotes mutant
T16879 749-756 NNS denotes alleles
T16885 757-759 IN denotes by
T16886 760-768 NNP denotes Southern
T16887 769-777 NN denotes blotting
T16888 777-778 . denotes .
T16889 778-966 sentence denotes The long (downstream) arm of homology was provided by a 7-kb HindIII-KpnI fragment, extending from intron 7 to intron 12, inserted between the PGK-Neo and thymidine kinase (tk) cassettes.
T16890 779-782 DT denotes The
T16892 783-787 JJ denotes long
T16893 788-789 -LRB- denotes (
T16894 789-799 JJ denotes downstream
T16895 799-800 -RRB- denotes )
T16891 801-804 NN denotes arm
T16897 805-807 IN denotes of
T16898 808-816 NN denotes homology
T16899 817-820 VBD denotes was
T16896 821-829 VBN denotes provided
T16900 830-832 IN denotes by
T16901 833-834 DT denotes a
T16903 835-836 CD denotes 7
T16905 836-837 HYPH denotes -
T16904 837-839 NN denotes kb
T16906 840-847 NN denotes HindIII
T16908 847-848 HYPH denotes -
T16907 848-852 NN denotes KpnI
T16902 853-861 NN denotes fragment
T16909 861-863 , denotes ,
T16910 863-872 VBG denotes extending
T16911 873-877 IN denotes from
T16912 878-884 NN denotes intron
T16913 885-886 CD denotes 7
T16914 887-889 IN denotes to
T16915 890-896 NN denotes intron
T16916 897-899 CD denotes 12
T16917 899-901 , denotes ,
T16918 901-909 VBN denotes inserted
T16919 910-917 IN denotes between
T16920 918-921 DT denotes the
T16922 922-925 NN denotes PGK
T16924 925-926 HYPH denotes -
T16923 926-929 NN denotes Neo
T16925 930-933 CC denotes and
T16926 934-943 NN denotes thymidine
T16927 944-950 NN denotes kinase
T16928 951-952 -LRB- denotes (
T16929 952-954 NN denotes tk
T16930 954-955 -RRB- denotes )
T16921 956-965 NNS denotes cassettes
T16931 965-966 . denotes .
R4917 T16716 T16717 det A,construct
R4918 T16717 T16719 nsubjpass construct,designed
R4919 T16718 T16717 compound targeting,construct
R4920 T16720 T16719 auxpass was,designed
R4921 T16721 T16722 aux to,replace
R4922 T16722 T16719 advcl replace,designed
R4923 T16723 T16724 det a,fragment
R4924 T16724 T16722 dobj fragment,replace
R4925 T16725 T16726 nummod 785,bp
R4926 T16726 T16724 compound bp,fragment
R4927 T16727 T16728 compound BamHI,HindIII
R4928 T16728 T16724 compound HindIII,fragment
R4929 T16729 T16728 punct -,HindIII
R4930 T16730 T16724 punct ", ",fragment
R4931 T16731 T16724 acl containing,fragment
R4932 T16732 T16731 dobj exon,containing
R4933 T16733 T16732 nummod 7,exon
R4934 T16734 T16732 prep of,exon
R4935 T16735 T16736 det the,gene
R4936 T16736 T16734 pobj gene,of
R4937 T16737 T16736 compound Capn2,gene
R4938 T16738 T16722 punct ", ",replace
R4939 T16739 T16722 prep with,replace
R4940 T16740 T16741 det the,cassette
R4941 T16741 T16739 pobj cassette,with
R4942 T16742 T16743 compound PGK,Neo
R4943 T16743 T16741 compound Neo,cassette
R4944 T16744 T16743 punct -,Neo
R4945 T16745 T16746 punct (,Figure
R4946 T16746 T16722 parataxis Figure,replace
R4947 T16747 T16746 nummod 1,Figure
R4948 T16748 T16746 punct ),Figure
R4949 T16749 T16719 punct .,designed
R4950 T16751 T16752 nsubj Exon,encodes
R4951 T16753 T16751 nummod 7,Exon
R4952 T16754 T16755 nummod 24,acids
R4953 T16755 T16752 dobj acids,encodes
R4954 T16756 T16755 compound amino,acids
R4955 T16757 T16752 prep in,encodes
R4956 T16758 T16759 det the,region
R4957 T16759 T16757 pobj region,in
R4958 T16760 T16761 amod active,site
R4959 T16761 T16759 compound site,region
R4960 T16762 T16761 punct -,site
R4961 T16763 T16752 punct ", ",encodes
R4962 T16764 T16752 prep including,encodes
R4963 T16765 T16764 pobj Asn286,including
R4964 T16766 T16765 punct ", ",Asn286
R4965 T16767 T16765 appos one,Asn286
R4966 T16768 T16767 prep of,one
R4967 T16769 T16770 det the,residues
R4968 T16770 T16768 pobj residues,of
R4969 T16771 T16770 amod catalytic,residues
R4970 T16772 T16770 compound triad,residues
R4971 T16773 T16752 punct .,encodes
R4972 T16775 T16776 det The,arm
R4973 T16776 T16781 nsubjpass arm,provided
R4974 T16777 T16776 amod short,arm
R4975 T16778 T16776 punct (,arm
R4976 T16779 T16776 amod upstream,arm
R4977 T16780 T16776 punct ),arm
R4978 T16782 T16776 prep of,arm
R4979 T16783 T16784 det the,construct
R4980 T16784 T16782 pobj construct,of
R4981 T16785 T16784 compound targeting,construct
R4982 T16786 T16781 auxpass was,provided
R4983 T16787 T16781 agent by,provided
R4984 T16788 T16789 det a,fragment
R4985 T16789 T16787 pobj fragment,by
R4986 T16790 T16791 nummod 2.7,kb
R4987 T16791 T16789 compound kb,fragment
R4988 T16792 T16791 punct -,kb
R4989 T16793 T16794 compound HindIII,BamHI
R4990 T16794 T16789 compound BamHI,fragment
R4991 T16795 T16794 punct -,BamHI
R4992 T16796 T16789 punct ", ",fragment
R4993 T16797 T16789 acl containing,fragment
R4994 T16798 T16799 nmod exons,5
R4995 T16799 T16797 dobj 5,containing
R4996 T16800 T16799 cc and,5
R4997 T16801 T16799 conj 6,5
R4998 T16802 T16789 punct ", ",fragment
R4999 T16803 T16804 dep which,inserted
R5000 T16804 T16789 relcl inserted,fragment
R5001 T16805 T16804 auxpass was,inserted
R5002 T16806 T16804 prep into,inserted
R5003 T16807 T16808 det the,vector
R5004 T16808 T16806 pobj vector,into
R5005 T16809 T16808 compound pPNT,vector
R5006 T16810 T16804 advmod upstream,inserted
R5007 T16811 T16810 prep of,upstream
R5008 T16812 T16813 det the,cassette
R5009 T16813 T16811 pobj cassette,of
R5010 T16814 T16815 compound PGK,Neo
R5011 T16815 T16813 compound Neo,cassette
R5012 T16816 T16815 punct -,Neo
R5013 T16817 T16818 punct [,48
R5014 T16818 T16804 parataxis 48,inserted
R5015 T16819 T16818 punct ],48
R5016 T16820 T16781 punct .,provided
R5017 T16822 T16823 prep During,abolished
R5018 T16824 T16822 pobj cloning,During
R5019 T16825 T16823 punct ", ",abolished
R5020 T16826 T16827 det the,site
R5021 T16827 T16823 nsubjpass site,abolished
R5022 T16828 T16827 compound BamHI,site
R5023 T16829 T16827 prep at,site
R5024 T16830 T16831 det the,end
R5025 T16831 T16829 pobj end,at
R5026 T16832 T16831 nummod 3,end
R5027 T16833 T16832 punct ',3
R5028 T16834 T16831 prep of,end
R5029 T16835 T16836 det the,arm
R5030 T16836 T16834 pobj arm,of
R5031 T16837 T16836 amod short,arm
R5032 T16838 T16823 auxpass was,abolished
R5033 T16839 T16823 punct .,abolished
R5034 T16841 T16842 det The,loss
R5035 T16842 T16843 nsubj loss,provided
R5036 T16844 T16842 prep of,loss
R5037 T16845 T16846 det this,site
R5038 T16846 T16844 pobj site,of
R5039 T16847 T16846 compound BamHI,site
R5040 T16848 T16842 prep in,loss
R5041 T16849 T16850 det the,allele
R5042 T16850 T16848 pobj allele,in
R5043 T16851 T16850 compound mutant,allele
R5044 T16852 T16842 punct ", ",loss
R5045 T16853 T16842 acl coupled,loss
R5046 T16854 T16853 prep with,coupled
R5047 T16855 T16856 det the,introduction
R5048 T16856 T16854 pobj introduction,with
R5049 T16857 T16856 prep of,introduction
R5050 T16858 T16859 det a,site
R5051 T16859 T16857 pobj site,of
R5052 T16860 T16859 amod new,site
R5053 T16861 T16859 compound BamHI,site
R5054 T16862 T16853 prep at,coupled
R5055 T16863 T16864 det the,end
R5056 T16864 T16862 pobj end,at
R5057 T16865 T16864 nummod 3,end
R5058 T16866 T16865 punct ',3
R5059 T16867 T16864 prep of,end
R5060 T16868 T16869 det the,cassette
R5061 T16869 T16867 pobj cassette,of
R5062 T16870 T16871 compound PGK,Neo
R5063 T16871 T16869 compound Neo,cassette
R5064 T16872 T16871 punct -,Neo
R5065 T16873 T16843 punct ", ",provided
R5066 T16874 T16875 det a,basis
R5067 T16875 T16843 dobj basis,provided
R5068 T16876 T16875 prep for,basis
R5069 T16877 T16876 pcomp distinguishing,for
R5070 T16878 T16879 det the,alleles
R5071 T16879 T16877 dobj alleles,distinguishing
R5072 T16880 T16881 amod wild,type
R5073 T16881 T16879 nmod type,alleles
R5074 T16882 T16881 punct -,type
R5075 T16883 T16881 cc and,type
R5076 T16884 T16881 conj mutant,type
R5077 T16885 T16877 prep by,distinguishing
R5078 T16886 T16887 compound Southern,blotting
R5079 T16887 T16885 pobj blotting,by
R5080 T16888 T16843 punct .,provided
R5081 T16890 T16891 det The,arm
R5082 T16891 T16896 nsubjpass arm,provided
R5083 T16892 T16891 amod long,arm
R5084 T16893 T16891 punct (,arm
R5085 T16894 T16891 amod downstream,arm
R5086 T16895 T16891 punct ),arm
R5087 T16897 T16891 prep of,arm
R5088 T16898 T16897 pobj homology,of
R5089 T16899 T16896 auxpass was,provided
R5090 T16900 T16896 agent by,provided
R5091 T16901 T16902 det a,fragment
R5092 T16902 T16900 pobj fragment,by
R5093 T16903 T16904 nummod 7,kb
R5094 T16904 T16902 compound kb,fragment
R5095 T16905 T16904 punct -,kb
R5096 T16906 T16907 compound HindIII,KpnI
R5097 T16907 T16902 compound KpnI,fragment
R5098 T16908 T16907 punct -,KpnI
R5099 T16909 T16902 punct ", ",fragment
R5100 T16910 T16902 acl extending,fragment
R5101 T16911 T16910 prep from,extending
R5102 T16912 T16911 pobj intron,from
R5103 T16913 T16912 nummod 7,intron
R5104 T16914 T16911 prep to,from
R5105 T16915 T16914 pobj intron,to
R5106 T16916 T16915 nummod 12,intron
R5107 T16917 T16902 punct ", ",fragment
R5108 T16918 T16902 acl inserted,fragment
R5109 T16919 T16918 prep between,inserted
R5110 T16920 T16921 det the,cassettes
R5111 T16921 T16919 pobj cassettes,between
R5112 T16922 T16923 nmod PGK,Neo
R5113 T16923 T16921 nmod Neo,cassettes
R5114 T16924 T16923 punct -,Neo
R5115 T16925 T16923 cc and,Neo
R5116 T16926 T16927 compound thymidine,kinase
R5117 T16927 T16923 conj kinase,Neo
R5118 T16928 T16927 punct (,kinase
R5119 T16929 T16927 appos tk,kinase
R5120 T16930 T16921 punct ),cassettes
R5121 T16931 T16896 punct .,provided