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PMC:1382200 / 11902-13046 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T21857 0-6 VBN denotes Nested
T21859 7-10 NN denotes PCR
T21858 11-19 NN denotes strategy
T21860 20-23 IN denotes for
T21861 24-34 NN denotes genotyping
T21862 35-37 IN denotes of
T21863 38-54 JJ denotes pre-implantation
T21864 55-62 NNS denotes embryos
T21865 62-63 . denotes .
T21866 63-199 sentence denotes Due to the scarcity of extractable genetic material, a nested PCR strategy was developed in order to genotype pre-implantation embryos.
T21867 64-67 IN denotes Due
T21869 68-70 IN denotes to
T21870 71-74 DT denotes the
T21871 75-83 NN denotes scarcity
T21872 84-86 IN denotes of
T21873 87-98 JJ denotes extractable
T21875 99-106 JJ denotes genetic
T21874 107-115 NN denotes material
T21876 115-117 , denotes ,
T21877 117-118 DT denotes a
T21879 119-125 VBN denotes nested
T21880 126-129 NN denotes PCR
T21878 130-138 NN denotes strategy
T21881 139-142 VBD denotes was
T21868 143-152 VBN denotes developed
T21882 153-155 IN denotes in
T21883 156-161 NN denotes order
T21884 162-164 TO denotes to
T21885 165-173 VB denotes genotype
T21886 174-190 JJ denotes pre-implantation
T21887 191-198 NNS denotes embryos
T21888 198-199 . denotes .
T21889 199-406 sentence denotes Separate reactions were used to amplify a 429 bp fragment from the wild-type allele and a 389 bp segment from the mutant allele, both spanning the 3' end of the short (upstream) arm of the targeting vector.
T21890 200-208 JJ denotes Separate
T21891 209-218 NNS denotes reactions
T21893 219-223 VBD denotes were
T21892 224-228 VBN denotes used
T21894 229-231 TO denotes to
T21895 232-239 VB denotes amplify
T21896 240-241 DT denotes a
T21898 242-245 CD denotes 429
T21899 246-248 NN denotes bp
T21897 249-257 NN denotes fragment
T21900 258-262 IN denotes from
T21901 263-266 DT denotes the
T21903 267-271 JJ denotes wild
T21905 271-272 HYPH denotes -
T21904 272-276 NN denotes type
T21902 277-283 NN denotes allele
T21906 284-287 CC denotes and
T21907 288-289 DT denotes a
T21909 290-293 CD denotes 389
T21910 294-296 NN denotes bp
T21908 297-304 NN denotes segment
T21911 305-309 IN denotes from
T21912 310-313 DT denotes the
T21914 314-320 NN denotes mutant
T21913 321-327 NN denotes allele
T21915 327-329 , denotes ,
T21916 329-333 DT denotes both
T21917 334-342 VBG denotes spanning
T21918 343-346 DT denotes the
T21920 347-348 CD denotes 3
T21921 348-349 SYM denotes '
T21919 350-353 NN denotes end
T21922 354-356 IN denotes of
T21923 357-360 DT denotes the
T21925 361-366 JJ denotes short
T21926 367-368 -LRB- denotes (
T21927 368-376 JJ denotes upstream
T21928 376-377 -RRB- denotes )
T21924 378-381 NN denotes arm
T21929 382-384 IN denotes of
T21930 385-388 DT denotes the
T21932 389-398 NN denotes targeting
T21931 399-405 NN denotes vector
T21933 405-406 . denotes .
T21934 406-544 sentence denotes In both reactions, a 213 bp sequence located within the short arm was co-amplified with the 'diagnostic' products as an internal control.
T21935 407-409 IN denotes In
T21937 410-414 DT denotes both
T21938 415-424 NNS denotes reactions
T21939 424-426 , denotes ,
T21940 426-427 DT denotes a
T21942 428-431 CD denotes 213
T21943 432-434 NN denotes bp
T21941 435-443 NN denotes sequence
T21944 444-451 VBN denotes located
T21945 452-458 IN denotes within
T21946 459-462 DT denotes the
T21948 463-468 JJ denotes short
T21947 469-472 NN denotes arm
T21949 473-476 VBD denotes was
T21936 477-489 VBN denotes co-amplified
T21950 490-494 IN denotes with
T21951 495-498 DT denotes the
T21953 499-500 `` denotes '
T21954 500-510 JJ denotes diagnostic
T21955 510-511 '' denotes '
T21952 512-520 NNS denotes products
T21956 521-523 IN denotes as
T21957 524-526 DT denotes an
T21959 527-535 JJ denotes internal
T21958 536-543 NN denotes control
T21960 543-544 . denotes .
T21961 544-682 sentence denotes The same sense primers were used to amplify 'diagnostic' sequences in both reactions, whereas the antisense primers were allele-specific.
T21962 545-548 DT denotes The
T21964 549-553 JJ denotes same
T21965 554-559 NN denotes sense
T21963 560-567 NNS denotes primers
T21967 568-572 VBD denotes were
T21966 573-577 VBN denotes used
T21968 578-580 TO denotes to
T21969 581-588 VB denotes amplify
T21970 589-590 `` denotes '
T21971 590-600 JJ denotes diagnostic
T21973 600-601 '' denotes '
T21972 602-611 NNS denotes sequences
T21974 612-614 IN denotes in
T21975 615-619 DT denotes both
T21976 620-629 NNS denotes reactions
T21977 629-631 , denotes ,
T21978 631-638 IN denotes whereas
T21980 639-642 DT denotes the
T21982 643-652 NN denotes antisense
T21981 653-660 NNS denotes primers
T21979 661-665 VBD denotes were
T21983 666-672 NN denotes allele
T21985 672-673 HYPH denotes -
T21984 673-681 JJ denotes specific
T21986 681-682 . denotes .
T21987 682-788 sentence denotes The primers, represented by triangles, are depicted in two (nested) sets for each of the three reactions.
T21988 683-686 DT denotes The
T21989 687-694 NNS denotes primers
T21991 694-696 , denotes ,
T21992 696-707 VBN denotes represented
T21993 708-710 IN denotes by
T21994 711-720 NNS denotes triangles
T21995 720-722 , denotes ,
T21996 722-725 VBP denotes are
T21990 726-734 VBN denotes depicted
T21997 735-737 IN denotes in
T21998 738-741 CD denotes two
T22000 742-743 -LRB- denotes (
T22001 743-749 VBN denotes nested
T22002 749-750 -RRB- denotes )
T21999 751-755 NNS denotes sets
T22003 756-759 IN denotes for
T22004 760-764 DT denotes each
T22005 765-767 IN denotes of
T22006 768-771 DT denotes the
T22008 772-777 CD denotes three
T22007 778-787 NNS denotes reactions
T22009 787-788 . denotes .
T22010 788-1005 sentence denotes Exons are represented by open, vertical rectangles, the PGK-Neo cassette by an open, horizontal rectangle, while thin vertical lines denote the boundaries of the short arm and the 5' end of the long (downstream) arm.
T22011 789-794 NNS denotes Exons
T22013 795-798 VBP denotes are
T22012 799-810 VBN denotes represented
T22015 811-813 IN denotes by
T22016 814-818 JJ denotes open
T22018 818-820 , denotes ,
T22019 820-828 JJ denotes vertical
T22017 829-839 NNS denotes rectangles
T22020 839-841 , denotes ,
T22021 841-844 DT denotes the
T22022 845-848 NN denotes PGK
T22024 848-849 HYPH denotes -
T22023 849-852 NN denotes Neo
T22014 853-861 NN denotes cassette
T22025 862-864 IN denotes by
T22026 865-867 DT denotes an
T22028 868-872 JJ denotes open
T22029 872-874 , denotes ,
T22030 874-884 JJ denotes horizontal
T22027 885-894 NN denotes rectangle
T22031 894-896 , denotes ,
T22032 896-901 IN denotes while
T22034 902-906 JJ denotes thin
T22036 907-915 JJ denotes vertical
T22035 916-921 NNS denotes lines
T22033 922-928 VBP denotes denote
T22037 929-932 DT denotes the
T22038 933-943 NNS denotes boundaries
T22039 944-946 IN denotes of
T22040 947-950 DT denotes the
T22042 951-956 JJ denotes short
T22041 957-960 NN denotes arm
T22043 961-964 CC denotes and
T22044 965-968 DT denotes the
T22046 969-970 CD denotes 5
T22047 970-971 SYM denotes '
T22045 972-975 NN denotes end
T22048 976-978 IN denotes of
T22049 979-982 DT denotes the
T22051 983-987 JJ denotes long
T22052 988-989 -LRB- denotes (
T22053 989-999 JJ denotes downstream
T22054 999-1000 -RRB- denotes )
T22050 1001-1004 NN denotes arm
T22055 1004-1005 . denotes .
T22056 1005-1144 sentence denotes A grey, horizontal rectangle delineates the segment of the wild-type allele that is replaced by the PGK-Neo cassette in the mutant allele.
T22057 1006-1007 DT denotes A
T22059 1008-1012 JJ denotes grey
T22060 1012-1014 , denotes ,
T22061 1014-1024 JJ denotes horizontal
T22058 1025-1034 NN denotes rectangle
T22062 1035-1045 VBZ denotes delineates
T22063 1046-1049 DT denotes the
T22064 1050-1057 NN denotes segment
T22065 1058-1060 IN denotes of
T22066 1061-1064 DT denotes the
T22068 1065-1069 JJ denotes wild
T22070 1069-1070 HYPH denotes -
T22069 1070-1074 NN denotes type
T22067 1075-1081 NN denotes allele
T22071 1082-1086 WDT denotes that
T22073 1087-1089 VBZ denotes is
T22072 1090-1098 VBN denotes replaced
T22074 1099-1101 IN denotes by
T22075 1102-1105 DT denotes the
T22077 1106-1109 NN denotes PGK
T22079 1109-1110 HYPH denotes -
T22078 1110-1113 NN denotes Neo
T22076 1114-1122 NN denotes cassette
T22080 1123-1125 IN denotes in
T22081 1126-1129 DT denotes the
T22083 1130-1136 NN denotes mutant
T22082 1137-1143 NN denotes allele
T22084 1143-1144 . denotes .
R6470 T21857 T21858 amod Nested,strategy
R6471 T21859 T21858 compound PCR,strategy
R6472 T21860 T21858 prep for,strategy
R6473 T21861 T21860 pobj genotyping,for
R6474 T21862 T21861 prep of,genotyping
R6475 T21863 T21864 amod pre-implantation,embryos
R6476 T21864 T21862 pobj embryos,of
R6477 T21865 T21858 punct .,strategy
R6478 T21867 T21868 prep Due,developed
R6479 T21869 T21867 pcomp to,Due
R6480 T21870 T21871 det the,scarcity
R6481 T21871 T21867 pobj scarcity,Due
R6482 T21872 T21871 prep of,scarcity
R6483 T21873 T21874 amod extractable,material
R6484 T21874 T21872 pobj material,of
R6485 T21875 T21874 amod genetic,material
R6486 T21876 T21868 punct ", ",developed
R6487 T21877 T21878 det a,strategy
R6488 T21878 T21868 nsubjpass strategy,developed
R6489 T21879 T21878 amod nested,strategy
R6490 T21880 T21878 compound PCR,strategy
R6491 T21881 T21868 auxpass was,developed
R6492 T21882 T21868 prep in,developed
R6493 T21883 T21882 pobj order,in
R6494 T21884 T21885 aux to,genotype
R6495 T21885 T21883 acl genotype,order
R6496 T21886 T21887 amod pre-implantation,embryos
R6497 T21887 T21885 dobj embryos,genotype
R6498 T21888 T21868 punct .,developed
R6499 T21890 T21891 amod Separate,reactions
R6500 T21891 T21892 nsubjpass reactions,used
R6501 T21893 T21892 auxpass were,used
R6502 T21894 T21895 aux to,amplify
R6503 T21895 T21892 advcl amplify,used
R6504 T21896 T21897 det a,fragment
R6505 T21897 T21895 dobj fragment,amplify
R6506 T21898 T21899 nummod 429,bp
R6507 T21899 T21897 compound bp,fragment
R6508 T21900 T21897 prep from,fragment
R6509 T21901 T21902 det the,allele
R6510 T21902 T21900 pobj allele,from
R6511 T21903 T21904 amod wild,type
R6512 T21904 T21902 compound type,allele
R6513 T21905 T21904 punct -,type
R6514 T21906 T21897 cc and,fragment
R6515 T21907 T21908 det a,segment
R6516 T21908 T21897 conj segment,fragment
R6517 T21909 T21910 nummod 389,bp
R6518 T21910 T21908 compound bp,segment
R6519 T21911 T21908 prep from,segment
R6520 T21912 T21913 det the,allele
R6521 T21913 T21911 pobj allele,from
R6522 T21914 T21913 compound mutant,allele
R6523 T21915 T21897 punct ", ",fragment
R6524 T21916 T21897 appos both,fragment
R6525 T21917 T21916 acl spanning,both
R6526 T21918 T21919 det the,end
R6527 T21919 T21917 dobj end,spanning
R6528 T21920 T21919 nummod 3,end
R6529 T21921 T21920 punct ',3
R6530 T21922 T21919 prep of,end
R6531 T21923 T21924 det the,arm
R6532 T21924 T21922 pobj arm,of
R6533 T21925 T21924 amod short,arm
R6534 T21926 T21927 punct (,upstream
R6535 T21927 T21924 parataxis upstream,arm
R6536 T21928 T21927 punct ),upstream
R6537 T21929 T21924 prep of,arm
R6538 T21930 T21931 det the,vector
R6539 T21931 T21929 pobj vector,of
R6540 T21932 T21931 compound targeting,vector
R6541 T21933 T21892 punct .,used
R6542 T21935 T21936 prep In,co-amplified
R6543 T21937 T21938 det both,reactions
R6544 T21938 T21935 pobj reactions,In
R6545 T21939 T21936 punct ", ",co-amplified
R6546 T21940 T21941 det a,sequence
R6547 T21941 T21936 nsubjpass sequence,co-amplified
R6548 T21942 T21943 nummod 213,bp
R6549 T21943 T21941 compound bp,sequence
R6550 T21944 T21941 acl located,sequence
R6551 T21945 T21944 prep within,located
R6552 T21946 T21947 det the,arm
R6553 T21947 T21945 pobj arm,within
R6554 T21948 T21947 amod short,arm
R6555 T21949 T21936 auxpass was,co-amplified
R6556 T21950 T21936 prep with,co-amplified
R6557 T21951 T21952 det the,products
R6558 T21952 T21950 pobj products,with
R6559 T21953 T21952 punct ',products
R6560 T21954 T21952 amod diagnostic,products
R6561 T21955 T21952 punct ',products
R6562 T21956 T21936 prep as,co-amplified
R6563 T21957 T21958 det an,control
R6564 T21958 T21956 pobj control,as
R6565 T21959 T21958 amod internal,control
R6566 T21960 T21936 punct .,co-amplified
R6567 T21962 T21963 det The,primers
R6568 T21963 T21966 nsubjpass primers,used
R6569 T21964 T21963 amod same,primers
R6570 T21965 T21963 compound sense,primers
R6571 T21967 T21966 auxpass were,used
R6572 T21968 T21969 aux to,amplify
R6573 T21969 T21966 advcl amplify,used
R6574 T21970 T21969 punct ',amplify
R6575 T21971 T21972 amod diagnostic,sequences
R6576 T21972 T21969 dobj sequences,amplify
R6577 T21973 T21972 punct ',sequences
R6578 T21974 T21972 prep in,sequences
R6579 T21975 T21976 det both,reactions
R6580 T21976 T21974 pobj reactions,in
R6581 T21977 T21969 punct ", ",amplify
R6582 T21978 T21979 mark whereas,were
R6583 T21979 T21969 advcl were,amplify
R6584 T21980 T21981 det the,primers
R6585 T21981 T21979 nsubj primers,were
R6586 T21982 T21981 compound antisense,primers
R6587 T21983 T21984 npadvmod allele,specific
R6588 T21984 T21979 acomp specific,were
R6589 T21985 T21984 punct -,specific
R6590 T21986 T21966 punct .,used
R6591 T21988 T21989 det The,primers
R6592 T21989 T21990 nsubjpass primers,depicted
R6593 T21991 T21989 punct ", ",primers
R6594 T21992 T21989 acl represented,primers
R6595 T21993 T21992 agent by,represented
R6596 T21994 T21993 pobj triangles,by
R6597 T21995 T21990 punct ", ",depicted
R6598 T21996 T21990 auxpass are,depicted
R6599 T21997 T21990 prep in,depicted
R6600 T21998 T21999 nummod two,sets
R6601 T21999 T21997 pobj sets,in
R6602 T22000 T21999 punct (,sets
R6603 T22001 T21999 amod nested,sets
R6604 T22002 T21999 punct ),sets
R6605 T22003 T21999 prep for,sets
R6606 T22004 T22003 pobj each,for
R6607 T22005 T22004 prep of,each
R6608 T22006 T22007 det the,reactions
R6609 T22007 T22005 pobj reactions,of
R6610 T22008 T22007 nummod three,reactions
R6611 T22009 T21990 punct .,depicted
R6612 T22011 T22012 nsubjpass Exons,represented
R6613 T22012 T22014 ccomp represented,cassette
R6614 T22013 T22012 auxpass are,represented
R6615 T22015 T22012 agent by,represented
R6616 T22016 T22017 amod open,rectangles
R6617 T22017 T22015 pobj rectangles,by
R6618 T22018 T22017 punct ", ",rectangles
R6619 T22019 T22017 amod vertical,rectangles
R6620 T22020 T22014 punct ", ",cassette
R6621 T22021 T22014 det the,cassette
R6622 T22022 T22023 compound PGK,Neo
R6623 T22023 T22014 compound Neo,cassette
R6624 T22024 T22023 punct -,Neo
R6625 T22025 T22014 agent by,cassette
R6626 T22026 T22027 det an,rectangle
R6627 T22027 T22025 pobj rectangle,by
R6628 T22028 T22027 amod open,rectangle
R6629 T22029 T22027 punct ", ",rectangle
R6630 T22030 T22027 amod horizontal,rectangle
R6631 T22031 T22014 punct ", ",cassette
R6632 T22032 T22033 mark while,denote
R6633 T22033 T22014 advcl denote,cassette
R6634 T22034 T22035 amod thin,lines
R6635 T22035 T22033 nsubj lines,denote
R6636 T22036 T22035 amod vertical,lines
R6637 T22037 T22038 det the,boundaries
R6638 T22038 T22033 dobj boundaries,denote
R6639 T22039 T22038 prep of,boundaries
R6640 T22040 T22041 det the,arm
R6641 T22041 T22039 pobj arm,of
R6642 T22042 T22041 amod short,arm
R6643 T22043 T22038 cc and,boundaries
R6644 T22044 T22045 det the,end
R6645 T22045 T22038 conj end,boundaries
R6646 T22046 T22045 nummod 5,end
R6647 T22047 T22046 punct ',5
R6648 T22048 T22045 prep of,end
R6649 T22049 T22050 det the,arm
R6650 T22050 T22048 pobj arm,of
R6651 T22051 T22050 amod long,arm
R6652 T22052 T22050 punct (,arm
R6653 T22053 T22050 amod downstream,arm
R6654 T22054 T22050 punct ),arm
R6655 T22055 T22014 punct .,cassette
R6656 T22057 T22058 det A,rectangle
R6657 T22058 T22062 nsubj rectangle,delineates
R6658 T22059 T22058 amod grey,rectangle
R6659 T22060 T22058 punct ", ",rectangle
R6660 T22061 T22058 amod horizontal,rectangle
R6661 T22063 T22064 det the,segment
R6662 T22064 T22062 dobj segment,delineates
R6663 T22065 T22064 prep of,segment
R6664 T22066 T22067 det the,allele
R6665 T22067 T22065 pobj allele,of
R6666 T22068 T22069 amod wild,type
R6667 T22069 T22067 compound type,allele
R6668 T22070 T22069 punct -,type
R6669 T22071 T22072 dep that,replaced
R6670 T22072 T22064 relcl replaced,segment
R6671 T22073 T22072 auxpass is,replaced
R6672 T22074 T22072 agent by,replaced
R6673 T22075 T22076 det the,cassette
R6674 T22076 T22074 pobj cassette,by
R6675 T22077 T22078 compound PGK,Neo
R6676 T22078 T22076 compound Neo,cassette
R6677 T22079 T22078 punct -,Neo
R6678 T22080 T22072 prep in,replaced
R6679 T22081 T22082 det the,allele
R6680 T22082 T22080 pobj allele,in
R6681 T22083 T22082 compound mutant,allele
R6682 T22084 T22062 punct .,delineates