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PMC:1315279 / 7494-30983 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T2061 9-14 NN denotes Sam68
T2063 15-17 VBZ denotes Is
T2062 18-27 VBN denotes Expressed
T2064 28-30 IN denotes in
T2065 31-34 DT denotes the
T2067 35-45 VBG denotes Developing
T2066 46-54 NN denotes Skeleton
T2068 55-57 IN denotes of
T2069 58-67 JJ denotes Embryonic
T2070 68-72 NNS denotes Mice
T2071 72-198 sentence denotes The Sam68 mRNA is known to be widely expressed [38], whereas its pattern of protein expression in vivo remains to be defined.
T2072 73-76 DT denotes The
T2074 77-82 NN denotes Sam68
T2073 83-87 NN denotes mRNA
T2076 88-90 VBZ denotes is
T2075 91-96 VBN denotes known
T2077 97-99 TO denotes to
T2079 100-102 VB denotes be
T2080 103-109 RB denotes widely
T2078 110-119 VBN denotes expressed
T2081 120-121 -LRB- denotes [
T2082 121-123 CD denotes 38
T2083 123-124 -RRB- denotes ]
T2084 124-126 , denotes ,
T2085 126-133 IN denotes whereas
T2087 134-137 PRP$ denotes its
T2088 138-145 NN denotes pattern
T2089 146-148 IN denotes of
T2090 149-156 NN denotes protein
T2091 157-167 NN denotes expression
T2092 168-170 FW denotes in
T2093 171-175 FW denotes vivo
T2086 176-183 VBZ denotes remains
T2094 184-186 TO denotes to
T2096 187-189 VB denotes be
T2095 190-197 VBN denotes defined
T2097 197-198 . denotes .
T2098 198-465 sentence denotes Sections of paraffin-embedded wild-type E14.5 and E16.5 embryonic mice were immunostained with the well-characterized AD1 anti-Sam68 antibody, raised in rabbits against an immunizing peptide that corresponds to amino acids 330 to 348 of the mouse Sam68 protein [46].
T2099 199-207 NNS denotes Sections
T2101 208-210 IN denotes of
T2102 211-219 NN denotes paraffin
T2104 219-220 HYPH denotes -
T2103 220-228 VBN denotes embedded
T2106 229-233 JJ denotes wild
T2108 233-234 HYPH denotes -
T2107 234-238 NN denotes type
T2109 239-244 NN denotes E14.5
T2110 245-248 CC denotes and
T2111 249-254 NN denotes E16.5
T2112 255-264 JJ denotes embryonic
T2105 265-269 NNS denotes mice
T2113 270-274 VBD denotes were
T2100 275-288 VBN denotes immunostained
T2114 289-293 IN denotes with
T2115 294-297 DT denotes the
T2117 298-302 RB denotes well
T2119 302-303 HYPH denotes -
T2118 303-316 VBN denotes characterized
T2120 317-320 NN denotes AD1
T2121 321-331 JJ denotes anti-Sam68
T2116 332-340 NN denotes antibody
T2122 340-342 , denotes ,
T2123 342-348 VBN denotes raised
T2124 349-351 IN denotes in
T2125 352-359 NNS denotes rabbits
T2126 360-367 IN denotes against
T2127 368-370 DT denotes an
T2129 371-381 VBG denotes immunizing
T2128 382-389 NN denotes peptide
T2130 390-394 WDT denotes that
T2131 395-406 VBZ denotes corresponds
T2132 407-409 IN denotes to
T2133 410-415 NN denotes amino
T2135 416-421 NNS denotes acids
T2134 422-425 CD denotes 330
T2136 426-428 IN denotes to
T2137 429-432 CD denotes 348
T2138 433-435 IN denotes of
T2139 436-439 DT denotes the
T2141 440-445 NN denotes mouse
T2142 446-451 NN denotes Sam68
T2140 452-459 NN denotes protein
T2143 460-461 -LRB- denotes [
T2144 461-463 CD denotes 46
T2145 463-464 -RRB- denotes ]
T2146 464-465 . denotes .
T2147 465-594 sentence denotes Sam68 immunoreactivity was observed in the skeleton and soft tissues of developing wild-type mice at E14.5 and E16.5 (Figure 1).
T2148 466-471 NN denotes Sam68
T2149 472-488 NN denotes immunoreactivity
T2151 489-492 VBD denotes was
T2150 493-501 VBN denotes observed
T2152 502-504 IN denotes in
T2153 505-508 DT denotes the
T2154 509-517 NN denotes skeleton
T2155 518-521 CC denotes and
T2156 522-526 JJ denotes soft
T2157 527-534 NNS denotes tissues
T2158 535-537 IN denotes of
T2159 538-548 VBG denotes developing
T2161 549-553 JJ denotes wild
T2163 553-554 HYPH denotes -
T2162 554-558 NN denotes type
T2160 559-563 NNS denotes mice
T2164 564-566 IN denotes at
T2165 567-572 NN denotes E14.5
T2166 573-576 CC denotes and
T2167 577-582 NN denotes E16.5
T2168 583-584 -LRB- denotes (
T2169 584-590 NN denotes Figure
T2170 591-592 CD denotes 1
T2171 592-593 -RRB- denotes )
T2172 593-594 . denotes .
T2173 594-966 sentence denotes Intense staining was seen in the nucleus of cells in the developing brain, heart, and small intestine (Figure 1B), as well as in chondrocytes in the nasal septum and the glandular tissue adjacent to the nasal cartilage (Figure 1C, panels A–D), in the vertebra and intervertebral discs (panels E–H) and in the epiphysis (panels I–K) and metaphysis (panel L) of long bones.
T2174 595-602 JJ denotes Intense
T2175 603-611 NN denotes staining
T2177 612-615 VBD denotes was
T2176 616-620 VBN denotes seen
T2178 621-623 IN denotes in
T2179 624-627 DT denotes the
T2180 628-635 NN denotes nucleus
T2181 636-638 IN denotes of
T2182 639-644 NNS denotes cells
T2183 645-647 IN denotes in
T2184 648-651 DT denotes the
T2186 652-662 VBG denotes developing
T2185 663-668 NN denotes brain
T2187 668-670 , denotes ,
T2188 670-675 NN denotes heart
T2189 675-677 , denotes ,
T2190 677-680 CC denotes and
T2191 681-686 JJ denotes small
T2192 687-696 NN denotes intestine
T2193 697-698 -LRB- denotes (
T2195 698-704 NN denotes Figure
T2194 705-707 NN denotes 1B
T2196 707-708 -RRB- denotes )
T2197 708-710 , denotes ,
T2198 710-712 RB denotes as
T2200 713-717 RB denotes well
T2199 718-720 IN denotes as
T2201 721-723 IN denotes in
T2202 724-736 NNS denotes chondrocytes
T2203 737-739 IN denotes in
T2204 740-743 DT denotes the
T2206 744-749 JJ denotes nasal
T2205 750-756 NN denotes septum
T2207 757-760 CC denotes and
T2208 761-764 DT denotes the
T2210 765-774 JJ denotes glandular
T2209 775-781 NN denotes tissue
T2211 782-790 JJ denotes adjacent
T2212 791-793 IN denotes to
T2213 794-797 DT denotes the
T2215 798-803 JJ denotes nasal
T2214 804-813 NN denotes cartilage
T2216 814-815 -LRB- denotes (
T2218 815-821 NN denotes Figure
T2217 822-824 NN denotes 1C
T2219 824-826 , denotes ,
T2220 826-832 NNS denotes panels
T2221 833-834 NN denotes A
T2222 834-835 SYM denotes –
T2223 835-836 NN denotes D
T2224 836-837 -RRB- denotes )
T2225 837-839 , denotes ,
T2226 839-841 IN denotes in
T2227 842-845 DT denotes the
T2228 846-854 NNS denotes vertebra
T2229 855-858 CC denotes and
T2230 859-873 JJ denotes intervertebral
T2231 874-879 NNS denotes discs
T2232 880-881 -LRB- denotes (
T2234 881-887 NNS denotes panels
T2233 888-889 NN denotes E
T2235 889-890 SYM denotes –
T2236 890-891 NN denotes H
T2237 891-892 -RRB- denotes )
T2238 893-896 CC denotes and
T2239 897-899 IN denotes in
T2240 900-903 DT denotes the
T2241 904-913 NN denotes epiphysis
T2242 914-915 -LRB- denotes (
T2244 915-921 NNS denotes panels
T2243 922-923 NN denotes I
T2245 923-924 SYM denotes –
T2246 924-925 NN denotes K
T2247 925-926 -RRB- denotes )
T2248 927-930 CC denotes and
T2249 931-941 NN denotes metaphysis
T2250 942-943 -LRB- denotes (
T2252 943-948 NN denotes panel
T2251 949-950 NN denotes L
T2253 950-951 -RRB- denotes )
T2254 952-954 IN denotes of
T2255 955-959 JJ denotes long
T2256 960-965 NNS denotes bones
T2257 965-966 . denotes .
T2258 966-1149 sentence denotes Nuclear staining was observed in proliferating chondrocytes and hypertrophic chondrocytes (Figure 1C, panel H), in osteoblasts (panel L; Dataset S1), and in osteoclasts (Dataset S1).
T2259 967-974 JJ denotes Nuclear
T2260 975-983 NN denotes staining
T2262 984-987 VBD denotes was
T2261 988-996 VBN denotes observed
T2263 997-999 IN denotes in
T2264 1000-1013 VBG denotes proliferating
T2265 1014-1026 NNS denotes chondrocytes
T2266 1027-1030 CC denotes and
T2267 1031-1043 JJ denotes hypertrophic
T2268 1044-1056 NNS denotes chondrocytes
T2269 1057-1058 -LRB- denotes (
T2271 1058-1064 NN denotes Figure
T2270 1065-1067 NN denotes 1C
T2272 1067-1069 , denotes ,
T2273 1069-1074 NN denotes panel
T2274 1075-1076 NN denotes H
T2275 1076-1077 -RRB- denotes )
T2276 1077-1079 , denotes ,
T2277 1079-1081 IN denotes in
T2278 1082-1093 NNS denotes osteoblasts
T2279 1094-1095 -LRB- denotes (
T2281 1095-1100 NN denotes panel
T2280 1101-1102 NN denotes L
T2282 1102-1103 : denotes ;
T2283 1104-1111 NN denotes Dataset
T2284 1112-1114 NN denotes S1
T2285 1114-1115 -RRB- denotes )
T2286 1115-1117 , denotes ,
T2287 1117-1120 CC denotes and
T2288 1121-1123 IN denotes in
T2289 1124-1135 NNS denotes osteoclasts
T2290 1136-1137 -LRB- denotes (
T2292 1137-1144 NN denotes Dataset
T2291 1145-1147 NN denotes S1
T2293 1147-1148 -RRB- denotes )
T2294 1148-1149 . denotes .
T2295 1149-1294 sentence denotes No staining was observed with preimmune serum or when the antiserum was preadsorbed with the immunizing peptide (Figure 1C, panels B, F, and J).
T2296 1150-1152 DT denotes No
T2297 1153-1161 NN denotes staining
T2299 1162-1165 VBD denotes was
T2298 1166-1174 VBN denotes observed
T2300 1175-1179 IN denotes with
T2301 1180-1189 JJ denotes preimmune
T2302 1190-1195 NN denotes serum
T2303 1196-1198 CC denotes or
T2304 1199-1203 WRB denotes when
T2306 1204-1207 DT denotes the
T2307 1208-1217 NN denotes antiserum
T2308 1218-1221 VBD denotes was
T2305 1222-1233 VBN denotes preadsorbed
T2309 1234-1238 IN denotes with
T2310 1239-1242 DT denotes the
T2312 1243-1253 VBG denotes immunizing
T2311 1254-1261 NN denotes peptide
T2313 1262-1263 -LRB- denotes (
T2315 1263-1269 NN denotes Figure
T2314 1270-1272 NN denotes 1C
T2316 1272-1274 , denotes ,
T2317 1274-1280 NNS denotes panels
T2318 1281-1282 NN denotes B
T2319 1282-1284 , denotes ,
T2320 1284-1285 NN denotes F
T2321 1285-1287 , denotes ,
T2322 1287-1290 CC denotes and
T2323 1291-1292 NN denotes J
T2324 1292-1293 -RRB- denotes )
T2325 1293-1294 . denotes .
T2326 1294-1470 sentence denotes Taken together, these data demonstrate that Sam68 protein is selectively expressed in the developing mouse embryo, with particularly elevated expression in cartilage and bone.
T2327 1295-1300 VBN denotes Taken
T2329 1301-1309 RB denotes together
T2330 1309-1311 , denotes ,
T2331 1311-1316 DT denotes these
T2332 1317-1321 NNS denotes data
T2328 1322-1333 VBP denotes demonstrate
T2333 1334-1338 IN denotes that
T2335 1339-1344 NN denotes Sam68
T2336 1345-1352 NN denotes protein
T2337 1353-1355 VBZ denotes is
T2338 1356-1367 RB denotes selectively
T2334 1368-1377 VBN denotes expressed
T2339 1378-1380 IN denotes in
T2340 1381-1384 DT denotes the
T2342 1385-1395 VBG denotes developing
T2343 1396-1401 NN denotes mouse
T2341 1402-1408 NN denotes embryo
T2344 1408-1410 , denotes ,
T2345 1410-1414 IN denotes with
T2346 1415-1427 RB denotes particularly
T2347 1428-1436 VBN denotes elevated
T2348 1437-1447 NN denotes expression
T2349 1448-1450 IN denotes in
T2350 1451-1460 NN denotes cartilage
T2351 1461-1464 CC denotes and
T2352 1465-1469 NN denotes bone
T2353 1469-1470 . denotes .
T2520 2671-2676 NN denotes Sam68
T2521 2677-2679 VBZ denotes is
T2522 2680-2683 RB denotes Not
T2523 2684-2693 JJ denotes Essential
T2524 2694-2697 IN denotes for
T2525 2698-2703 NN denotes Mouse
T2526 2704-2715 NN denotes Development
T2527 2715-2931 sentence denotes To define the physiologic role of Sam68, we generated Sam68-deficient mice by targeted disruption of exons 4 and 5 of the sam68 gene, which encode the functional region of the KH-type RNA binding domain (Figure 2A).
T2528 2716-2718 TO denotes To
T2529 2719-2725 VB denotes define
T2531 2726-2729 DT denotes the
T2533 2730-2741 JJ denotes physiologic
T2532 2742-2746 NN denotes role
T2534 2747-2749 IN denotes of
T2535 2750-2755 NN denotes Sam68
T2536 2755-2757 , denotes ,
T2537 2757-2759 PRP denotes we
T2530 2760-2769 VBD denotes generated
T2538 2770-2775 NN denotes Sam68
T2540 2775-2776 HYPH denotes -
T2539 2776-2785 JJ denotes deficient
T2541 2786-2790 NNS denotes mice
T2542 2791-2793 IN denotes by
T2543 2794-2802 VBN denotes targeted
T2544 2803-2813 NN denotes disruption
T2545 2814-2816 IN denotes of
T2546 2817-2822 NNS denotes exons
T2547 2823-2824 CD denotes 4
T2548 2825-2828 CC denotes and
T2549 2829-2830 CD denotes 5
T2550 2831-2833 IN denotes of
T2551 2834-2837 DT denotes the
T2553 2838-2843 NN denotes sam68
T2552 2844-2848 NN denotes gene
T2554 2848-2850 , denotes ,
T2555 2850-2855 WDT denotes which
T2556 2856-2862 VBP denotes encode
T2557 2863-2866 DT denotes the
T2559 2867-2877 JJ denotes functional
T2558 2878-2884 NN denotes region
T2560 2885-2887 IN denotes of
T2561 2888-2891 DT denotes the
T2563 2892-2894 NN denotes KH
T2565 2894-2895 HYPH denotes -
T2564 2895-2899 NN denotes type
T2566 2900-2903 NN denotes RNA
T2567 2904-2911 NN denotes binding
T2562 2912-2918 NN denotes domain
T2568 2919-2920 -LRB- denotes (
T2570 2920-2926 NN denotes Figure
T2569 2927-2929 NN denotes 2A
T2571 2929-2930 -RRB- denotes )
T2572 2930-2931 . denotes .
T2573 2931-3065 sentence denotes The integrity of the targeted allele was verified by Southern blot analysis (Figure 2B) and by PCR of genomic DNA (unpublished data).
T2574 2932-2935 DT denotes The
T2575 2936-2945 NN denotes integrity
T2577 2946-2948 IN denotes of
T2578 2949-2952 DT denotes the
T2580 2953-2961 VBN denotes targeted
T2579 2962-2968 NN denotes allele
T2581 2969-2972 VBD denotes was
T2576 2973-2981 VBN denotes verified
T2582 2982-2984 IN denotes by
T2583 2985-2993 NNP denotes Southern
T2584 2994-2998 NN denotes blot
T2585 2999-3007 NN denotes analysis
T2586 3008-3009 -LRB- denotes (
T2588 3009-3015 NN denotes Figure
T2587 3016-3018 NN denotes 2B
T2589 3018-3019 -RRB- denotes )
T2590 3020-3023 CC denotes and
T2591 3024-3026 IN denotes by
T2592 3027-3030 NN denotes PCR
T2593 3031-3033 IN denotes of
T2594 3034-3041 JJ denotes genomic
T2595 3042-3045 NN denotes DNA
T2596 3046-3047 -LRB- denotes (
T2598 3047-3058 JJ denotes unpublished
T2597 3059-3063 NNS denotes data
T2599 3063-3064 -RRB- denotes )
T2600 3064-3065 . denotes .
T2601 3065-3365 sentence denotes Sam68 transcripts encoded by exons 1 to 5 were absent as evidenced by RT-PCR (Dataset S2), and Sam68−/− mice were devoid of Sam68 protein, as visualized by immunoblotting with anti-Sam68 AD1 and SC-333 antibodies or control normal rabbit serum or anti-Sam68 AD1 preabsorbed with peptide (Figure 2C).
T2602 3066-3071 NN denotes Sam68
T2603 3072-3083 NNS denotes transcripts
T2605 3084-3091 VBN denotes encoded
T2606 3092-3094 IN denotes by
T2607 3095-3100 NNS denotes exons
T2608 3101-3102 CD denotes 1
T2609 3103-3105 IN denotes to
T2610 3106-3107 CD denotes 5
T2604 3108-3112 VBD denotes were
T2611 3113-3119 JJ denotes absent
T2612 3120-3122 IN denotes as
T2613 3123-3132 VBN denotes evidenced
T2614 3133-3135 IN denotes by
T2615 3136-3138 NN denotes RT
T2617 3138-3139 HYPH denotes -
T2616 3139-3142 NN denotes PCR
T2618 3143-3144 -LRB- denotes (
T2620 3144-3151 NN denotes Dataset
T2619 3152-3154 NN denotes S2
T2621 3154-3155 -RRB- denotes )
T2622 3155-3157 , denotes ,
T2623 3157-3160 CC denotes and
T2624 3161-3166 NN denotes Sam68
T2626 3166-3167 SYM denotes −
T2627 3167-3168 HYPH denotes /
T2628 3168-3169 SYM denotes −
T2625 3170-3174 NNS denotes mice
T2629 3175-3179 VBD denotes were
T2630 3180-3186 JJ denotes devoid
T2631 3187-3189 IN denotes of
T2632 3190-3195 NN denotes Sam68
T2633 3196-3203 NN denotes protein
T2634 3203-3205 , denotes ,
T2635 3205-3207 IN denotes as
T2636 3208-3218 VBN denotes visualized
T2637 3219-3221 IN denotes by
T2638 3222-3236 VBG denotes immunoblotting
T2639 3237-3241 IN denotes with
T2640 3242-3252 JJ denotes anti-Sam68
T2641 3253-3256 NN denotes AD1
T2643 3257-3260 CC denotes and
T2644 3261-3263 NN denotes SC
T2645 3263-3264 HYPH denotes -
T2646 3264-3267 CD denotes 333
T2642 3268-3278 NNS denotes antibodies
T2647 3279-3281 CC denotes or
T2648 3282-3289 NN denotes control
T2650 3290-3296 JJ denotes normal
T2651 3297-3303 NN denotes rabbit
T2649 3304-3309 NN denotes serum
T2652 3310-3312 CC denotes or
T2653 3313-3323 JJ denotes anti-Sam68
T2654 3324-3327 NN denotes AD1
T2655 3328-3339 VBN denotes preabsorbed
T2656 3340-3344 IN denotes with
T2657 3345-3352 NN denotes peptide
T2658 3353-3354 -LRB- denotes (
T2660 3354-3360 NN denotes Figure
T2659 3361-3363 NN denotes 2C
T2661 3363-3364 -RRB- denotes )
T2662 3364-3365 . denotes .
T2663 3365-3473 sentence denotes SC-333 is a rabbit anti-Sam68 antibody that was raised against the C-terminal 20 amino acids of Sam68 [47].
T2664 3366-3368 NN denotes SC
T2666 3368-3369 HYPH denotes -
T2667 3369-3372 CD denotes 333
T2665 3373-3375 VBZ denotes is
T2668 3376-3377 DT denotes a
T2670 3378-3384 NN denotes rabbit
T2671 3385-3395 JJ denotes anti-Sam68
T2669 3396-3404 NN denotes antibody
T2672 3405-3409 WDT denotes that
T2674 3410-3413 VBD denotes was
T2673 3414-3420 VBN denotes raised
T2675 3421-3428 IN denotes against
T2676 3429-3432 DT denotes the
T2678 3433-3434 NN denotes C
T2680 3434-3435 HYPH denotes -
T2679 3435-3443 JJ denotes terminal
T2681 3444-3446 CD denotes 20
T2682 3447-3452 NN denotes amino
T2677 3453-3458 NNS denotes acids
T2683 3459-3461 IN denotes of
T2684 3462-3467 NN denotes Sam68
T2685 3468-3469 -LRB- denotes [
T2686 3469-3471 CD denotes 47
T2687 3471-3472 -RRB- denotes ]
T2688 3472-3473 . denotes .
T2689 3473-3540 sentence denotes These data confirmed the generation of a mouse deficient in Sam68.
T2690 3474-3479 DT denotes These
T2691 3480-3484 NNS denotes data
T2692 3485-3494 VBD denotes confirmed
T2693 3495-3498 DT denotes the
T2694 3499-3509 NN denotes generation
T2695 3510-3512 IN denotes of
T2696 3513-3514 DT denotes a
T2697 3515-3520 NN denotes mouse
T2698 3521-3530 JJ denotes deficient
T2699 3531-3533 IN denotes in
T2700 3534-3539 NN denotes Sam68
T2701 3539-3540 . denotes .
T2702 3540-3652 sentence denotes The genotypes of offspring from heterozygote intercrosses exhibited a Mendelian segregation at E18.5 (Table 1).
T2703 3541-3544 DT denotes The
T2704 3545-3554 NNS denotes genotypes
T2706 3555-3557 IN denotes of
T2707 3558-3567 NN denotes offspring
T2708 3568-3572 IN denotes from
T2709 3573-3585 NN denotes heterozygote
T2710 3586-3598 NNS denotes intercrosses
T2705 3599-3608 VBD denotes exhibited
T2711 3609-3610 DT denotes a
T2713 3611-3620 JJ denotes Mendelian
T2712 3621-3632 NN denotes segregation
T2714 3633-3635 IN denotes at
T2715 3636-3641 NN denotes E18.5
T2716 3642-3643 -LRB- denotes (
T2717 3643-3648 NN denotes Table
T2718 3649-3650 CD denotes 1
T2719 3650-3651 -RRB- denotes )
T2720 3651-3652 . denotes .
T2721 3652-3750 sentence denotes Despite the lack of visible deformity, many of the Sam68−/− pups died at birth of unknown causes.
T2722 3653-3660 IN denotes Despite
T2724 3661-3664 DT denotes the
T2725 3665-3669 NN denotes lack
T2726 3670-3672 IN denotes of
T2727 3673-3680 JJ denotes visible
T2728 3681-3690 NN denotes deformity
T2729 3690-3692 , denotes ,
T2730 3692-3696 JJ denotes many
T2731 3697-3699 IN denotes of
T2732 3700-3703 DT denotes the
T2734 3704-3709 NN denotes Sam68
T2735 3709-3710 SYM denotes −
T2736 3710-3711 HYPH denotes /
T2737 3711-3712 SYM denotes −
T2733 3713-3717 NNS denotes pups
T2723 3718-3722 VBD denotes died
T2738 3723-3725 IN denotes at
T2739 3726-3731 NN denotes birth
T2740 3732-3734 IN denotes of
T2741 3735-3742 JJ denotes unknown
T2742 3743-3749 NNS denotes causes
T2743 3749-3750 . denotes .
T2744 3750-3873 sentence denotes Sam68+/- mice were phenotypically normal and Sam68−/− pups that survived the perinatal period invariably lived to old age.
T2745 3751-3756 NN denotes Sam68
T2747 3756-3757 SYM denotes +
T2748 3757-3758 HYPH denotes /
T2749 3758-3759 SYM denotes -
T2746 3760-3764 NNS denotes mice
T2750 3765-3769 VBD denotes were
T2751 3770-3784 RB denotes phenotypically
T2752 3785-3791 JJ denotes normal
T2753 3792-3795 CC denotes and
T2754 3796-3801 NN denotes Sam68
T2756 3801-3802 SYM denotes −
T2757 3802-3803 HYPH denotes /
T2758 3803-3804 SYM denotes −
T2755 3805-3809 NNS denotes pups
T2760 3810-3814 WDT denotes that
T2761 3815-3823 VBD denotes survived
T2762 3824-3827 DT denotes the
T2764 3828-3837 JJ denotes perinatal
T2763 3838-3844 NN denotes period
T2765 3845-3855 RB denotes invariably
T2759 3856-3861 VBD denotes lived
T2766 3862-3864 IN denotes to
T2767 3865-3868 JJ denotes old
T2768 3869-3872 NN denotes age
T2769 3872-3873 . denotes .
T2770 3873-4058 sentence denotes Despite evidence that Sam68 mRNA is widely expressed and phosphorylated in mitosis [41,42], the Sam68−/− mice did not develop tumors and showed no immunologic or other major illnesses.
T2771 3874-3881 IN denotes Despite
T2773 3882-3890 NN denotes evidence
T2774 3891-3895 IN denotes that
T2776 3896-3901 NN denotes Sam68
T2777 3902-3906 NN denotes mRNA
T2778 3907-3909 VBZ denotes is
T2779 3910-3916 RB denotes widely
T2775 3917-3926 VBN denotes expressed
T2780 3927-3930 CC denotes and
T2781 3931-3945 VBN denotes phosphorylated
T2782 3946-3948 IN denotes in
T2783 3949-3956 NN denotes mitosis
T2784 3957-3958 -LRB- denotes [
T2786 3958-3960 CD denotes 41
T2787 3960-3961 , denotes ,
T2785 3961-3963 CD denotes 42
T2788 3963-3964 -RRB- denotes ]
T2789 3964-3966 , denotes ,
T2790 3966-3969 DT denotes the
T2792 3970-3975 NN denotes Sam68
T2793 3975-3976 SYM denotes −
T2794 3976-3977 HYPH denotes /
T2795 3977-3978 SYM denotes −
T2791 3979-3983 NNS denotes mice
T2796 3984-3987 VBD denotes did
T2797 3988-3991 RB denotes not
T2772 3992-3999 VB denotes develop
T2798 4000-4006 NNS denotes tumors
T2799 4007-4010 CC denotes and
T2800 4011-4017 VBD denotes showed
T2801 4018-4020 DT denotes no
T2803 4021-4032 JJ denotes immunologic
T2804 4033-4035 CC denotes or
T2805 4036-4041 JJ denotes other
T2806 4042-4047 JJ denotes major
T2802 4048-4057 NNS denotes illnesses
T2807 4057-4058 . denotes .
T2808 4058-4197 sentence denotes Sam68−/− mice did, however, have difficulty breeding due to male infertility and the females rarely provided adequate care to their young.
T2809 4059-4064 NN denotes Sam68
T2811 4064-4065 SYM denotes −
T2812 4065-4066 HYPH denotes /
T2813 4066-4067 SYM denotes −
T2810 4068-4072 NNS denotes mice
T2815 4073-4076 VBD denotes did
T2816 4076-4078 , denotes ,
T2817 4078-4085 RB denotes however
T2818 4085-4087 , denotes ,
T2814 4087-4091 VB denotes have
T2819 4092-4102 NN denotes difficulty
T2820 4103-4111 VBG denotes breeding
T2821 4112-4115 IN denotes due
T2822 4116-4118 IN denotes to
T2823 4119-4123 JJ denotes male
T2824 4124-4135 NN denotes infertility
T2825 4136-4139 CC denotes and
T2826 4140-4143 DT denotes the
T2827 4144-4151 NNS denotes females
T2829 4152-4158 RB denotes rarely
T2828 4159-4167 VBD denotes provided
T2830 4168-4176 JJ denotes adequate
T2831 4177-4181 NN denotes care
T2832 4182-4184 IN denotes to
T2833 4185-4190 PRP$ denotes their
T2834 4191-4196 JJ denotes young
T2835 4196-4197 . denotes .
T3035 5426-5431 NN denotes Sam68
T3036 5432-5442 NN denotes Deficiency
T3037 5443-5451 VBZ denotes Protects
T3038 5452-5456 NNS denotes Mice
T3039 5457-5461 IN denotes from
T3040 5462-5465 NN denotes Age
T3042 5465-5466 HYPH denotes -
T3041 5466-5473 VBN denotes Related
T3044 5474-5478 NN denotes Bone
T3043 5479-5483 NN denotes Loss
T3045 5483-5652 sentence denotes Src null animals are known to have bone metabolism defects [48], and since Sam68 is a substrate of Src, we decided to analyze the Sam68 mice for skeletal abnormalities.
T3046 5484-5487 NN denotes Src
T3048 5488-5492 JJ denotes null
T3047 5493-5500 NNS denotes animals
T3050 5501-5504 VBP denotes are
T3049 5505-5510 VBN denotes known
T3051 5511-5513 TO denotes to
T3052 5514-5518 VB denotes have
T3053 5519-5523 NN denotes bone
T3054 5524-5534 NN denotes metabolism
T3055 5535-5542 NNS denotes defects
T3056 5543-5544 -LRB- denotes [
T3057 5544-5546 CD denotes 48
T3058 5546-5547 -RRB- denotes ]
T3059 5547-5549 , denotes ,
T3060 5549-5552 CC denotes and
T3061 5553-5558 IN denotes since
T3063 5559-5564 NN denotes Sam68
T3062 5565-5567 VBZ denotes is
T3065 5568-5569 DT denotes a
T3066 5570-5579 NN denotes substrate
T3067 5580-5582 IN denotes of
T3068 5583-5586 NN denotes Src
T3069 5586-5588 , denotes ,
T3070 5588-5590 PRP denotes we
T3064 5591-5598 VBD denotes decided
T3071 5599-5601 TO denotes to
T3072 5602-5609 VB denotes analyze
T3073 5610-5613 DT denotes the
T3075 5614-5619 NN denotes Sam68
T3074 5620-5624 NNS denotes mice
T3076 5625-5628 IN denotes for
T3077 5629-5637 JJ denotes skeletal
T3078 5638-5651 NNS denotes abnormalities
T3079 5651-5652 . denotes .
T3080 5652-5758 sentence denotes Cohorts of Sam68+/+ and Sam68−/− mice were euthanized at 4 and 12 months of age for skeletal phenotyping.
T3081 5653-5660 NNS denotes Cohorts
T3083 5661-5663 IN denotes of
T3084 5664-5669 NN denotes Sam68
T3086 5669-5670 SYM denotes +
T3087 5670-5671 HYPH denotes /
T3088 5671-5672 SYM denotes +
T3089 5673-5676 CC denotes and
T3090 5677-5682 NN denotes Sam68
T3091 5682-5683 SYM denotes −
T3092 5683-5684 HYPH denotes /
T3093 5684-5685 SYM denotes −
T3085 5686-5690 NNS denotes mice
T3094 5691-5695 VBD denotes were
T3082 5696-5706 VBN denotes euthanized
T3095 5707-5709 IN denotes at
T3096 5710-5711 CD denotes 4
T3098 5712-5715 CC denotes and
T3099 5716-5718 CD denotes 12
T3097 5719-5725 NNS denotes months
T3100 5726-5728 IN denotes of
T3101 5729-5732 NN denotes age
T3102 5733-5736 IN denotes for
T3103 5737-5745 JJ denotes skeletal
T3104 5746-5757 NN denotes phenotyping
T3105 5757-5758 . denotes .
T3106 5758-5920 sentence denotes To minimize differences in the bone phenotype that might arise secondary to gender or weight differences, we selected age-matched female mice for these analyses.
T3107 5759-5761 TO denotes To
T3108 5762-5770 VB denotes minimize
T3110 5771-5782 NNS denotes differences
T3111 5783-5785 IN denotes in
T3112 5786-5789 DT denotes the
T3114 5790-5794 NN denotes bone
T3113 5795-5804 NN denotes phenotype
T3115 5805-5809 WDT denotes that
T3117 5810-5815 MD denotes might
T3116 5816-5821 VB denotes arise
T3118 5822-5831 JJ denotes secondary
T3119 5832-5834 IN denotes to
T3120 5835-5841 NN denotes gender
T3122 5842-5844 CC denotes or
T3123 5845-5851 NN denotes weight
T3121 5852-5863 NNS denotes differences
T3124 5863-5865 , denotes ,
T3125 5865-5867 PRP denotes we
T3109 5868-5876 VBD denotes selected
T3126 5877-5880 NN denotes age
T3128 5880-5881 HYPH denotes -
T3127 5881-5888 VBN denotes matched
T3130 5889-5895 JJ denotes female
T3129 5896-5900 NNS denotes mice
T3131 5901-5904 IN denotes for
T3132 5905-5910 DT denotes these
T3133 5911-5919 NNS denotes analyses
T3134 5919-5920 . denotes .
T3135 5920-6168 sentence denotes The female mice demonstrated similar increases in body weight, although 4-month-old Sam68−/− mice weighed less than Sam68+/+ mice, and similar changes in bone lengths in the axial and appendicular skeleton between 4 and 12 months of age (Table 2).
T3136 5921-5924 DT denotes The
T3138 5925-5931 JJ denotes female
T3137 5932-5936 NNS denotes mice
T3139 5937-5949 VBD denotes demonstrated
T3140 5950-5957 JJ denotes similar
T3141 5958-5967 NNS denotes increases
T3142 5968-5970 IN denotes in
T3143 5971-5975 NN denotes body
T3144 5976-5982 NN denotes weight
T3145 5982-5984 , denotes ,
T3147 5984-5992 IN denotes although
T3148 5993-5994 CD denotes 4
T3150 5994-5995 HYPH denotes -
T3149 5995-6000 NN denotes month
T3152 6000-6001 HYPH denotes -
T3151 6001-6004 JJ denotes old
T3154 6005-6010 NN denotes Sam68
T3155 6010-6011 SYM denotes −
T3156 6011-6012 HYPH denotes /
T3157 6012-6013 SYM denotes −
T3153 6014-6018 NNS denotes mice
T3146 6019-6026 VBD denotes weighed
T3158 6027-6031 JJR denotes less
T3159 6032-6036 IN denotes than
T3160 6037-6042 NN denotes Sam68
T3162 6042-6043 SYM denotes +
T3163 6043-6044 HYPH denotes /
T3164 6044-6045 SYM denotes +
T3161 6046-6050 NNS denotes mice
T3165 6050-6052 , denotes ,
T3166 6052-6055 CC denotes and
T3167 6056-6063 JJ denotes similar
T3168 6064-6071 NNS denotes changes
T3169 6072-6074 IN denotes in
T3170 6075-6079 NN denotes bone
T3171 6080-6087 NNS denotes lengths
T3172 6088-6090 IN denotes in
T3173 6091-6094 DT denotes the
T3175 6095-6100 JJ denotes axial
T3176 6101-6104 CC denotes and
T3177 6105-6117 JJ denotes appendicular
T3174 6118-6126 NN denotes skeleton
T3178 6127-6134 IN denotes between
T3179 6135-6136 CD denotes 4
T3181 6137-6140 CC denotes and
T3182 6141-6143 CD denotes 12
T3180 6144-6150 NNS denotes months
T3183 6151-6153 IN denotes of
T3184 6154-6157 NN denotes age
T3185 6158-6159 -LRB- denotes (
T3186 6159-6164 NN denotes Table
T3187 6165-6166 CD denotes 2
T3188 6166-6167 -RRB- denotes )
T3189 6167-6168 . denotes .
T3190 6168-6539 sentence denotes Faxitron radiography (Faxitron X-ray Corporation, Wheeling, Illinois, United States) (Figure 3A) and micro–computed tomography (CT) (Figure 3B) revealed significant cortical thinning (arrow) and a reduction in metaphyseal bone (asterisk) in the distal femora of 12-month-old Sam68+/+ mice compared with 4-month-old mice of either genotype and 12-month-old Sam68−/− mice.
T3191 6169-6177 NNP denotes Faxitron
T3192 6178-6189 NN denotes radiography
T3194 6190-6191 -LRB- denotes (
T3196 6191-6199 NNP denotes Faxitron
T3197 6200-6201 NN denotes X
T3199 6201-6202 HYPH denotes -
T3198 6202-6205 NN denotes ray
T3195 6206-6217 NNP denotes Corporation
T3200 6217-6219 , denotes ,
T3201 6219-6227 NNP denotes Wheeling
T3202 6227-6229 , denotes ,
T3203 6229-6237 NNP denotes Illinois
T3204 6237-6239 , denotes ,
T3205 6239-6245 NNP denotes United
T3206 6246-6252 NNP denotes States
T3207 6252-6253 -RRB- denotes )
T3208 6254-6255 -LRB- denotes (
T3210 6255-6261 NN denotes Figure
T3209 6262-6264 NN denotes 3A
T3211 6264-6265 -RRB- denotes )
T3212 6266-6269 CC denotes and
T3213 6270-6284 VBN denotes micro–computed
T3214 6285-6295 NN denotes tomography
T3215 6296-6297 -LRB- denotes (
T3216 6297-6299 NN denotes CT
T3217 6299-6300 -RRB- denotes )
T3218 6301-6302 -LRB- denotes (
T3220 6302-6308 NN denotes Figure
T3219 6309-6311 NN denotes 3B
T3221 6311-6312 -RRB- denotes )
T3193 6313-6321 VBD denotes revealed
T3222 6322-6333 JJ denotes significant
T3224 6334-6342 JJ denotes cortical
T3223 6343-6351 NN denotes thinning
T3225 6352-6353 -LRB- denotes (
T3226 6353-6358 NN denotes arrow
T3227 6358-6359 -RRB- denotes )
T3228 6360-6363 CC denotes and
T3229 6364-6365 DT denotes a
T3230 6366-6375 NN denotes reduction
T3231 6376-6378 IN denotes in
T3232 6379-6390 JJ denotes metaphyseal
T3233 6391-6395 NN denotes bone
T3234 6396-6397 -LRB- denotes (
T3235 6397-6405 NN denotes asterisk
T3236 6405-6406 -RRB- denotes )
T3237 6407-6409 IN denotes in
T3238 6410-6413 DT denotes the
T3240 6414-6420 JJ denotes distal
T3239 6421-6427 NNS denotes femora
T3241 6428-6430 IN denotes of
T3242 6431-6433 CD denotes 12
T3244 6433-6434 HYPH denotes -
T3243 6434-6439 NN denotes month
T3246 6439-6440 HYPH denotes -
T3245 6440-6443 JJ denotes old
T3248 6444-6449 NN denotes Sam68
T3249 6449-6450 SYM denotes +
T3250 6450-6451 HYPH denotes /
T3251 6451-6452 SYM denotes +
T3247 6453-6457 NNS denotes mice
T3252 6458-6466 VBN denotes compared
T3253 6467-6471 IN denotes with
T3254 6472-6473 CD denotes 4
T3256 6473-6474 HYPH denotes -
T3255 6474-6479 NN denotes month
T3258 6479-6480 HYPH denotes -
T3257 6480-6483 JJ denotes old
T3259 6484-6488 NNS denotes mice
T3260 6489-6491 IN denotes of
T3261 6492-6498 CC denotes either
T3262 6499-6507 NN denotes genotype
T3263 6508-6511 CC denotes and
T3264 6512-6514 CD denotes 12
T3266 6514-6515 HYPH denotes -
T3265 6515-6520 NN denotes month
T3268 6520-6521 HYPH denotes -
T3267 6521-6524 JJ denotes old
T3270 6525-6530 NN denotes Sam68
T3271 6530-6531 SYM denotes −
T3272 6531-6532 HYPH denotes /
T3273 6532-6533 SYM denotes −
T3269 6534-6538 NNS denotes mice
T3274 6538-6539 . denotes .
T3275 6539-6726 sentence denotes Similar reductions in trabecular bone were shown by Faxitron and micro-CT in the fifth lumbar vertebrae of the 12-month-old Sam68+/+ mice but not in the Sam68−/− mice (unpublished data).
T3276 6540-6547 JJ denotes Similar
T3277 6548-6558 NNS denotes reductions
T3279 6559-6561 IN denotes in
T3280 6562-6572 JJ denotes trabecular
T3281 6573-6577 NN denotes bone
T3282 6578-6582 VBD denotes were
T3278 6583-6588 VBN denotes shown
T3283 6589-6591 IN denotes by
T3284 6592-6600 NNP denotes Faxitron
T3285 6601-6604 CC denotes and
T3286 6605-6613 NN denotes micro-CT
T3287 6614-6616 IN denotes in
T3288 6617-6620 DT denotes the
T3290 6621-6626 JJ denotes fifth
T3291 6627-6633 NN denotes lumbar
T3289 6634-6643 NNS denotes vertebrae
T3292 6644-6646 IN denotes of
T3293 6647-6650 DT denotes the
T3295 6651-6653 CD denotes 12
T3297 6653-6654 HYPH denotes -
T3296 6654-6659 NN denotes month
T3299 6659-6660 HYPH denotes -
T3298 6660-6663 JJ denotes old
T3300 6664-6669 NN denotes Sam68
T3301 6669-6670 SYM denotes +
T3302 6670-6671 HYPH denotes /
T3303 6671-6672 SYM denotes +
T3294 6673-6677 NNS denotes mice
T3304 6678-6681 CC denotes but
T3305 6682-6685 RB denotes not
T3306 6686-6688 IN denotes in
T3307 6689-6692 DT denotes the
T3309 6693-6698 NN denotes Sam68
T3310 6698-6699 SYM denotes −
T3311 6699-6700 HYPH denotes /
T3312 6700-6701 SYM denotes −
T3308 6702-6706 NNS denotes mice
T3313 6707-6708 -LRB- denotes (
T3315 6708-6719 JJ denotes unpublished
T3314 6720-6724 NNS denotes data
T3316 6724-6725 -RRB- denotes )
T3317 6725-6726 . denotes .
T3318 6726-7009 sentence denotes Total body bone mineral content (BMC), quantified with a Lunar PIXImus mouse densitometer (GE-Lunar, Madison, Wisconsin, United States), increased in both Sam68+/+ and Sam68−/− mice between 4 months and 12 months of age, but only reached significance in the Sam68−/− mice (Table 2).
T3319 6727-6732 JJ denotes Total
T3321 6733-6737 NN denotes body
T3322 6738-6742 NN denotes bone
T3323 6743-6750 NN denotes mineral
T3320 6751-6758 NN denotes content
T3325 6759-6760 -LRB- denotes (
T3326 6760-6763 NN denotes BMC
T3327 6763-6764 -RRB- denotes )
T3328 6764-6766 , denotes ,
T3329 6766-6776 VBN denotes quantified
T3330 6777-6781 IN denotes with
T3331 6782-6783 DT denotes a
T3333 6784-6789 JJ denotes Lunar
T3334 6790-6797 NNP denotes PIXImus
T3335 6798-6803 NN denotes mouse
T3332 6804-6816 NN denotes densitometer
T3336 6817-6818 -LRB- denotes (
T3338 6818-6820 NNP denotes GE
T3339 6820-6821 HYPH denotes -
T3337 6821-6826 NNP denotes Lunar
T3340 6826-6828 , denotes ,
T3341 6828-6835 NNP denotes Madison
T3342 6835-6837 , denotes ,
T3343 6837-6846 NNP denotes Wisconsin
T3344 6846-6848 , denotes ,
T3345 6848-6854 NNP denotes United
T3346 6855-6861 NNP denotes States
T3347 6861-6862 -RRB- denotes )
T3348 6862-6864 , denotes ,
T3324 6864-6873 VBD denotes increased
T3349 6874-6876 IN denotes in
T3350 6877-6881 CC denotes both
T3351 6882-6887 NN denotes Sam68
T3353 6887-6888 SYM denotes +
T3354 6888-6889 HYPH denotes /
T3355 6889-6890 SYM denotes +
T3356 6891-6894 CC denotes and
T3357 6895-6900 NN denotes Sam68
T3358 6900-6901 SYM denotes −
T3359 6901-6902 HYPH denotes /
T3360 6902-6903 SYM denotes −
T3352 6904-6908 NNS denotes mice
T3361 6909-6916 IN denotes between
T3362 6917-6918 CD denotes 4
T3363 6919-6925 NNS denotes months
T3364 6926-6929 CC denotes and
T3365 6930-6932 CD denotes 12
T3366 6933-6939 NNS denotes months
T3367 6940-6942 IN denotes of
T3368 6943-6946 NN denotes age
T3369 6946-6948 , denotes ,
T3370 6948-6951 CC denotes but
T3371 6952-6956 RB denotes only
T3372 6957-6964 VBD denotes reached
T3373 6965-6977 NN denotes significance
T3374 6978-6980 IN denotes in
T3375 6981-6984 DT denotes the
T3377 6985-6990 NN denotes Sam68
T3378 6990-6991 SYM denotes −
T3379 6991-6992 HYPH denotes /
T3380 6992-6993 SYM denotes −
T3376 6994-6998 NNS denotes mice
T3381 6999-7000 -LRB- denotes (
T3382 7000-7005 NN denotes Table
T3383 7006-7007 CD denotes 2
T3384 7007-7008 -RRB- denotes )
T3385 7008-7009 . denotes .
T3386 7009-7103 sentence denotes Similarly, a greater increase in BMC was seen in the femur and vertebra in the Sam68−/− mice.
T3387 7010-7019 RB denotes Similarly
T3389 7019-7021 , denotes ,
T3390 7021-7022 DT denotes a
T3392 7023-7030 JJR denotes greater
T3391 7031-7039 NN denotes increase
T3393 7040-7042 IN denotes in
T3394 7043-7046 NN denotes BMC
T3395 7047-7050 VBD denotes was
T3388 7051-7055 VBN denotes seen
T3396 7056-7058 IN denotes in
T3397 7059-7062 DT denotes the
T3398 7063-7068 NN denotes femur
T3399 7069-7072 CC denotes and
T3400 7073-7081 NN denotes vertebra
T3401 7082-7084 IN denotes in
T3402 7085-7088 DT denotes the
T3404 7089-7094 NN denotes Sam68
T3405 7094-7095 SYM denotes −
T3406 7095-7096 HYPH denotes /
T3407 7096-7097 SYM denotes −
T3403 7098-7102 NNS denotes mice
T3408 7102-7103 . denotes .
T3409 7103-7309 sentence denotes Bone mineral density (BMD) remained constant or decreased in the wild type mice but increased significantly in the total body and in the femoral and vertebral regions of interest in the Sam68−/− (Table 2).
T3410 7104-7108 NN denotes Bone
T3411 7109-7116 NN denotes mineral
T3412 7117-7124 NN denotes density
T3414 7125-7126 -LRB- denotes (
T3415 7126-7129 NN denotes BMD
T3416 7129-7130 -RRB- denotes )
T3413 7131-7139 VBD denotes remained
T3417 7140-7148 JJ denotes constant
T3418 7149-7151 CC denotes or
T3419 7152-7161 VBN denotes decreased
T3420 7162-7164 IN denotes in
T3421 7165-7168 DT denotes the
T3423 7169-7173 JJ denotes wild
T3424 7174-7178 NN denotes type
T3422 7179-7183 NNS denotes mice
T3425 7184-7187 CC denotes but
T3426 7188-7197 VBD denotes increased
T3427 7198-7211 RB denotes significantly
T3428 7212-7214 IN denotes in
T3429 7215-7218 DT denotes the
T3431 7219-7224 JJ denotes total
T3430 7225-7229 NN denotes body
T3432 7230-7233 CC denotes and
T3433 7234-7236 IN denotes in
T3434 7237-7240 DT denotes the
T3436 7241-7248 JJ denotes femoral
T3437 7249-7252 CC denotes and
T3438 7253-7262 JJ denotes vertebral
T3435 7263-7270 NNS denotes regions
T3439 7271-7273 IN denotes of
T3440 7274-7282 NN denotes interest
T3441 7283-7285 IN denotes in
T3442 7286-7289 DT denotes the
T3443 7290-7295 NN denotes Sam68
T3444 7295-7296 SYM denotes −
T3445 7296-7297 HYPH denotes /
T3446 7297-7298 SYM denotes −
T3447 7299-7300 -LRB- denotes (
T3448 7300-7305 NN denotes Table
T3449 7306-7307 CD denotes 2
T3450 7307-7308 -RRB- denotes )
T3451 7308-7309 . denotes .
T3452 7309-7448 sentence denotes These data showed that Sam68−/− mice continued to thrive and accrue bone in the axial and appendicular skeleton for longer than 12 months.
T3453 7310-7315 DT denotes These
T3454 7316-7320 NNS denotes data
T3455 7321-7327 VBD denotes showed
T3456 7328-7332 IN denotes that
T3458 7333-7338 NN denotes Sam68
T3460 7338-7339 SYM denotes −
T3461 7339-7340 HYPH denotes /
T3462 7340-7341 SYM denotes −
T3459 7342-7346 NNS denotes mice
T3457 7347-7356 VBD denotes continued
T3463 7357-7359 TO denotes to
T3464 7360-7366 VB denotes thrive
T3465 7367-7370 CC denotes and
T3466 7371-7377 VB denotes accrue
T3467 7378-7382 NN denotes bone
T3468 7383-7385 IN denotes in
T3469 7386-7389 DT denotes the
T3471 7390-7395 JJ denotes axial
T3472 7396-7399 CC denotes and
T3473 7400-7412 JJ denotes appendicular
T3470 7413-7421 NN denotes skeleton
T3474 7422-7425 IN denotes for
T3475 7426-7432 JJR denotes longer
T3477 7433-7437 IN denotes than
T3476 7438-7440 CD denotes 12
T3478 7441-7447 NNS denotes months
T3479 7447-7448 . denotes .
T3480 7448-7594 sentence denotes This was in contrast to the situation in age-matched, littermate controls in which a significant amount of bone was lost over the same timeframe.
T3481 7449-7453 DT denotes This
T3482 7454-7457 VBD denotes was
T3483 7458-7460 IN denotes in
T3484 7461-7469 NN denotes contrast
T3485 7470-7472 IN denotes to
T3486 7473-7476 DT denotes the
T3487 7477-7486 NN denotes situation
T3488 7487-7489 IN denotes in
T3489 7490-7493 NN denotes age
T3491 7493-7494 HYPH denotes -
T3490 7494-7501 VBN denotes matched
T3493 7501-7503 , denotes ,
T3494 7503-7513 NN denotes littermate
T3492 7514-7522 NNS denotes controls
T3495 7523-7525 IN denotes in
T3497 7526-7531 WDT denotes which
T3498 7532-7533 DT denotes a
T3500 7534-7545 JJ denotes significant
T3499 7546-7552 NN denotes amount
T3501 7553-7555 IN denotes of
T3502 7556-7560 NN denotes bone
T3503 7561-7564 VBD denotes was
T3496 7565-7569 VBN denotes lost
T3504 7570-7574 IN denotes over
T3505 7575-7578 DT denotes the
T3507 7579-7583 JJ denotes same
T3506 7584-7593 NN denotes timeframe
T3508 7593-7594 . denotes .
T3587 8881-8886 CD denotes Three
T3589 8886-8887 HYPH denotes -
T3588 8887-8898 JJ denotes Dimensional
T3590 8899-8911 NN denotes Architecture
T3592 8912-8914 IN denotes of
T3593 8915-8919 NN denotes Bone
T3594 8920-8922 VBZ denotes Is
T3591 8923-8932 VBN denotes Preserved
T3595 8933-8935 IN denotes in
T3596 8936-8940 VBN denotes Aged
T3598 8941-8946 NN denotes Sam68
T3599 8946-8947 SYM denotes −
T3600 8947-8948 HYPH denotes /
T3601 8948-8949 SYM denotes −
T3597 8950-8954 NNS denotes Mice
T3602 8954-9176 sentence denotes Bone loss and compromised architecture are characteristic features of the skeletons of aged C57BL/6 mice and resemble the clinical features of age-related bone loss in humans that can predispose an individual to fracture.
T3603 8955-8959 NN denotes Bone
T3604 8960-8964 NN denotes loss
T3606 8965-8968 CC denotes and
T3607 8969-8980 VBN denotes compromised
T3608 8981-8993 NN denotes architecture
T3605 8994-8997 VBP denotes are
T3609 8998-9012 JJ denotes characteristic
T3610 9013-9021 NNS denotes features
T3611 9022-9024 IN denotes of
T3612 9025-9028 DT denotes the
T3613 9029-9038 NNS denotes skeletons
T3614 9039-9041 IN denotes of
T3615 9042-9046 JJ denotes aged
T3617 9047-9052 NN denotes C57BL
T3618 9052-9053 HYPH denotes /
T3619 9053-9054 CD denotes 6
T3616 9055-9059 NNS denotes mice
T3620 9060-9063 CC denotes and
T3621 9064-9072 VBP denotes resemble
T3622 9073-9076 DT denotes the
T3624 9077-9085 JJ denotes clinical
T3623 9086-9094 NNS denotes features
T3625 9095-9097 IN denotes of
T3626 9098-9101 NN denotes age
T3628 9101-9102 HYPH denotes -
T3627 9102-9109 VBN denotes related
T3630 9110-9114 NN denotes bone
T3629 9115-9119 NN denotes loss
T3631 9120-9122 IN denotes in
T3632 9123-9129 NNS denotes humans
T3633 9130-9134 WDT denotes that
T3635 9135-9138 MD denotes can
T3634 9139-9149 VB denotes predispose
T3636 9150-9152 DT denotes an
T3637 9153-9163 NN denotes individual
T3638 9164-9166 IN denotes to
T3639 9167-9175 NN denotes fracture
T3640 9175-9176 . denotes .
T3641 9176-9482 sentence denotes Quantification of the micro-CT data shown in Figure 3B confirmed the reduction in bone volume compared with tissue volume (BV/TV; Figure 4A, left) in the 12-month-old Sam68+/+ mice (hatched bars) compared with 4-month-old mice of both genotypes (solid bars) and 12-month-old Sam68−/− mice (stippled bars).
T3642 9177-9191 NN denotes Quantification
T3644 9192-9194 IN denotes of
T3645 9195-9198 DT denotes the
T3647 9199-9207 NN denotes micro-CT
T3646 9208-9212 NNS denotes data
T3648 9213-9218 VBN denotes shown
T3649 9219-9221 IN denotes in
T3650 9222-9228 NN denotes Figure
T3651 9229-9231 NN denotes 3B
T3643 9232-9241 VBD denotes confirmed
T3652 9242-9245 DT denotes the
T3653 9246-9255 NN denotes reduction
T3654 9256-9258 IN denotes in
T3655 9259-9263 NN denotes bone
T3656 9264-9270 NN denotes volume
T3657 9271-9279 VBN denotes compared
T3658 9280-9284 IN denotes with
T3659 9285-9291 NN denotes tissue
T3660 9292-9298 NN denotes volume
T3661 9299-9300 -LRB- denotes (
T3663 9300-9302 NN denotes BV
T3665 9302-9303 HYPH denotes /
T3664 9303-9305 NN denotes TV
T3666 9305-9306 : denotes ;
T3667 9307-9313 NN denotes Figure
T3662 9314-9316 NN denotes 4A
T3668 9316-9318 , denotes ,
T3669 9318-9322 JJ denotes left
T3670 9322-9323 -RRB- denotes )
T3671 9324-9326 IN denotes in
T3672 9327-9330 DT denotes the
T3674 9331-9333 CD denotes 12
T3676 9333-9334 HYPH denotes -
T3675 9334-9339 NN denotes month
T3678 9339-9340 HYPH denotes -
T3677 9340-9343 JJ denotes old
T3679 9344-9349 NN denotes Sam68
T3680 9349-9350 SYM denotes +
T3681 9350-9351 HYPH denotes /
T3682 9351-9352 SYM denotes +
T3673 9353-9357 NNS denotes mice
T3683 9358-9359 -LRB- denotes (
T3685 9359-9366 VBN denotes hatched
T3684 9367-9371 NNS denotes bars
T3686 9371-9372 -RRB- denotes )
T3687 9373-9381 VBN denotes compared
T3688 9382-9386 IN denotes with
T3689 9387-9388 CD denotes 4
T3691 9388-9389 HYPH denotes -
T3690 9389-9394 NN denotes month
T3693 9394-9395 HYPH denotes -
T3692 9395-9398 JJ denotes old
T3694 9399-9403 NNS denotes mice
T3695 9404-9406 IN denotes of
T3696 9407-9411 DT denotes both
T3697 9412-9421 NNS denotes genotypes
T3698 9422-9423 -LRB- denotes (
T3700 9423-9428 JJ denotes solid
T3699 9429-9433 NNS denotes bars
T3701 9433-9434 -RRB- denotes )
T3702 9435-9438 CC denotes and
T3703 9439-9441 CD denotes 12
T3705 9441-9442 HYPH denotes -
T3704 9442-9447 NN denotes month
T3707 9447-9448 HYPH denotes -
T3706 9448-9451 JJ denotes old
T3709 9452-9457 NN denotes Sam68
T3710 9457-9458 SYM denotes −
T3711 9458-9459 HYPH denotes /
T3712 9459-9460 SYM denotes −
T3708 9461-9465 NNS denotes mice
T3713 9466-9467 -LRB- denotes (
T3715 9467-9475 VBN denotes stippled
T3714 9476-9480 NNS denotes bars
T3716 9480-9481 -RRB- denotes )
T3717 9481-9482 . denotes .
T3718 9482-9679 sentence denotes This was associated with a significant increase (p < 0.01; denoted by the asterisks) in the structure model index, which measures the ratio of plate-like to rod-like structures (Figure 4A, right).
T3719 9483-9487 DT denotes This
T3721 9488-9491 VBD denotes was
T3720 9492-9502 VBN denotes associated
T3722 9503-9507 IN denotes with
T3723 9508-9509 DT denotes a
T3725 9510-9521 JJ denotes significant
T3724 9522-9530 NN denotes increase
T3726 9531-9532 -LRB- denotes (
T3728 9532-9533 NN denotes p
T3730 9534-9535 SYM denotes <
T3729 9536-9540 CD denotes 0.01
T3731 9540-9541 : denotes ;
T3727 9542-9549 VBN denotes denoted
T3732 9550-9552 IN denotes by
T3733 9553-9556 DT denotes the
T3734 9557-9566 NNS denotes asterisks
T3735 9566-9567 -RRB- denotes )
T3736 9568-9570 IN denotes in
T3737 9571-9574 DT denotes the
T3739 9575-9584 NN denotes structure
T3740 9585-9590 NN denotes model
T3738 9591-9596 NN denotes index
T3741 9596-9598 , denotes ,
T3742 9598-9603 WDT denotes which
T3743 9604-9612 VBZ denotes measures
T3744 9613-9616 DT denotes the
T3745 9617-9622 NN denotes ratio
T3746 9623-9625 IN denotes of
T3747 9626-9631 NN denotes plate
T3749 9631-9632 HYPH denotes -
T3748 9632-9636 JJ denotes like
T3750 9637-9639 IN denotes to
T3751 9640-9643 NN denotes rod
T3753 9643-9644 HYPH denotes -
T3752 9644-9648 JJ denotes like
T3754 9649-9659 NNS denotes structures
T3755 9660-9661 -LRB- denotes (
T3757 9661-9667 NN denotes Figure
T3756 9668-9670 NN denotes 4A
T3758 9670-9672 , denotes ,
T3759 9672-9677 NN denotes right
T3760 9677-9678 -RRB- denotes )
T3761 9678-9679 . denotes .
T3762 9679-9914 sentence denotes A quantifiable increase in the mean trabecular separation (Tb.Sp) (unpublished data) in 12-month-old Sam68+/+ mice was due to an increase in the percentage of spaces falling in the range of 350 to 700 μm (Figure 4B, red hatched line).
T3763 9680-9681 DT denotes A
T3765 9682-9694 JJ denotes quantifiable
T3764 9695-9703 NN denotes increase
T3767 9704-9706 IN denotes in
T3768 9707-9710 DT denotes the
T3770 9711-9715 NN denotes mean
T3771 9716-9726 JJ denotes trabecular
T3769 9727-9737 NN denotes separation
T3772 9738-9739 -LRB- denotes (
T3773 9739-9744 NN denotes Tb.Sp
T3774 9744-9745 -RRB- denotes )
T3775 9746-9747 -LRB- denotes (
T3777 9747-9758 JJ denotes unpublished
T3776 9759-9763 NNS denotes data
T3778 9763-9764 -RRB- denotes )
T3779 9765-9767 IN denotes in
T3780 9768-9770 CD denotes 12
T3782 9770-9771 HYPH denotes -
T3781 9771-9776 NN denotes month
T3784 9776-9777 HYPH denotes -
T3783 9777-9780 JJ denotes old
T3786 9781-9786 NN denotes Sam68
T3787 9786-9787 SYM denotes +
T3788 9787-9788 HYPH denotes /
T3789 9788-9789 SYM denotes +
T3785 9790-9794 NNS denotes mice
T3766 9795-9798 VBD denotes was
T3790 9799-9802 IN denotes due
T3791 9803-9805 IN denotes to
T3792 9806-9808 DT denotes an
T3793 9809-9817 NN denotes increase
T3794 9818-9820 IN denotes in
T3795 9821-9824 DT denotes the
T3796 9825-9835 NN denotes percentage
T3797 9836-9838 IN denotes of
T3798 9839-9845 NNS denotes spaces
T3799 9846-9853 VBG denotes falling
T3800 9854-9856 IN denotes in
T3801 9857-9860 DT denotes the
T3802 9861-9866 NN denotes range
T3803 9867-9869 IN denotes of
T3804 9870-9873 CD denotes 350
T3806 9874-9876 IN denotes to
T3805 9877-9880 CD denotes 700
T3807 9881-9883 NN denotes μm
T3808 9884-9885 -LRB- denotes (
T3810 9885-9891 NN denotes Figure
T3809 9892-9894 NN denotes 4B
T3811 9894-9896 , denotes ,
T3812 9896-9899 JJ denotes red
T3814 9900-9907 VBN denotes hatched
T3813 9908-9912 NN denotes line
T3815 9912-9913 -RRB- denotes )
T3816 9913-9914 . denotes .
T3817 9914-10008 sentence denotes Trabecular thickness remained constant among the different groups of mice (unpublished data).
T3818 9915-9925 JJ denotes Trabecular
T3819 9926-9935 NN denotes thickness
T3820 9936-9944 VBD denotes remained
T3821 9945-9953 JJ denotes constant
T3822 9954-9959 IN denotes among
T3823 9960-9963 DT denotes the
T3825 9964-9973 JJ denotes different
T3824 9974-9980 NNS denotes groups
T3826 9981-9983 IN denotes of
T3827 9984-9988 NNS denotes mice
T3828 9989-9990 -LRB- denotes (
T3830 9990-10001 JJ denotes unpublished
T3829 10002-10006 NNS denotes data
T3831 10006-10007 -RRB- denotes )
T3832 10007-10008 . denotes .
T3833 10008-10196 sentence denotes In effect, this meant that there were fewer trabeculae, rather than equivalent numbers of thin trabeculae, in the 12-month-old Sam68+/+ mice compared with any of the other groups of mice.
T3834 10009-10011 IN denotes In
T3836 10012-10018 NN denotes effect
T3837 10018-10020 , denotes ,
T3838 10020-10024 DT denotes this
T3835 10025-10030 VBD denotes meant
T3839 10031-10035 IN denotes that
T3841 10036-10041 EX denotes there
T3840 10042-10046 VBD denotes were
T3842 10047-10052 JJR denotes fewer
T3843 10053-10063 NNS denotes trabeculae
T3844 10063-10065 , denotes ,
T3845 10065-10071 RB denotes rather
T3846 10072-10076 IN denotes than
T3847 10077-10087 JJ denotes equivalent
T3848 10088-10095 NNS denotes numbers
T3849 10096-10098 IN denotes of
T3850 10099-10103 JJ denotes thin
T3851 10104-10114 NNS denotes trabeculae
T3852 10114-10116 , denotes ,
T3853 10116-10118 IN denotes in
T3854 10119-10122 DT denotes the
T3856 10123-10125 CD denotes 12
T3858 10125-10126 HYPH denotes -
T3857 10126-10131 NN denotes month
T3860 10131-10132 HYPH denotes -
T3859 10132-10135 JJ denotes old
T3861 10136-10141 NN denotes Sam68
T3862 10141-10142 SYM denotes +
T3863 10142-10143 HYPH denotes /
T3864 10143-10144 SYM denotes +
T3855 10145-10149 NNS denotes mice
T3865 10150-10158 VBN denotes compared
T3866 10159-10163 IN denotes with
T3867 10164-10167 DT denotes any
T3868 10168-10170 IN denotes of
T3869 10171-10174 DT denotes the
T3871 10175-10180 JJ denotes other
T3870 10181-10187 NNS denotes groups
T3872 10188-10190 IN denotes of
T3873 10191-10195 NNS denotes mice
T3874 10195-10196 . denotes .
T4163 11086-11090 NN denotes Bone
T4164 11091-11101 NN denotes Remodeling
T4166 11102-11104 VBZ denotes Is
T4165 11105-11114 VBN denotes Preserved
T4167 11115-11117 IN denotes in
T4168 11118-11122 VBN denotes Aged
T4170 11123-11128 NN denotes Sam68
T4171 11128-11129 SYM denotes −
T4172 11129-11130 HYPH denotes /
T4173 11130-11131 SYM denotes −
T4169 11132-11136 NNS denotes Mice
T4174 11136-11353 sentence denotes To further define the mechanisms involved in the preservation of bone mass in aged Sam68−/− mice, we prepared sections from plastic-embedded femora and tibia to evaluate osteoblast and osteoclast activity (Figure 5).
T4175 11137-11139 TO denotes To
T4177 11140-11147 RB denotes further
T4176 11148-11154 VB denotes define
T4179 11155-11158 DT denotes the
T4180 11159-11169 NNS denotes mechanisms
T4181 11170-11178 VBN denotes involved
T4182 11179-11181 IN denotes in
T4183 11182-11185 DT denotes the
T4184 11186-11198 NN denotes preservation
T4185 11199-11201 IN denotes of
T4186 11202-11206 NN denotes bone
T4187 11207-11211 NN denotes mass
T4188 11212-11214 IN denotes in
T4189 11215-11219 JJ denotes aged
T4191 11220-11225 NN denotes Sam68
T4192 11225-11226 SYM denotes −
T4193 11226-11227 HYPH denotes /
T4194 11227-11228 SYM denotes −
T4190 11229-11233 NNS denotes mice
T4195 11233-11235 , denotes ,
T4196 11235-11237 PRP denotes we
T4178 11238-11246 VBD denotes prepared
T4197 11247-11255 NNS denotes sections
T4198 11256-11260 IN denotes from
T4199 11261-11268 NN denotes plastic
T4201 11268-11269 HYPH denotes -
T4200 11269-11277 VBN denotes embedded
T4202 11278-11284 NNS denotes femora
T4203 11285-11288 CC denotes and
T4204 11289-11294 NN denotes tibia
T4205 11295-11297 TO denotes to
T4206 11298-11306 VB denotes evaluate
T4207 11307-11317 NN denotes osteoblast
T4209 11318-11321 CC denotes and
T4210 11322-11332 NN denotes osteoclast
T4208 11333-11341 NN denotes activity
T4211 11342-11343 -LRB- denotes (
T4212 11343-11349 NN denotes Figure
T4213 11350-11351 CD denotes 5
T4214 11351-11352 -RRB- denotes )
T4215 11352-11353 . denotes .
T4216 11353-11605 sentence denotes In situ enzyme histochemical staining for alkaline phosphatase (ALP; brown stain) activity was used as a biomarker for osteoblasts and tartrate-resistant ALP (tartrate-resistant acid phosphatase [TRAP]; red stain) activity as a marker for osteoclasts.
T4217 11354-11356 FW denotes In
T4218 11357-11361 FW denotes situ
T4220 11362-11368 NN denotes enzyme
T4221 11369-11382 JJ denotes histochemical
T4219 11383-11391 NN denotes staining
T4223 11392-11395 IN denotes for
T4224 11396-11404 NN denotes alkaline
T4225 11405-11416 NN denotes phosphatase
T4227 11417-11418 -LRB- denotes (
T4228 11418-11421 NN denotes ALP
T4229 11421-11422 : denotes ;
T4231 11423-11428 JJ denotes brown
T4230 11429-11434 NN denotes stain
T4232 11434-11435 -RRB- denotes )
T4226 11436-11444 NN denotes activity
T4233 11445-11448 VBD denotes was
T4222 11449-11453 VBN denotes used
T4234 11454-11456 IN denotes as
T4235 11457-11458 DT denotes a
T4236 11459-11468 NN denotes biomarker
T4237 11469-11472 IN denotes for
T4238 11473-11484 NNS denotes osteoblasts
T4239 11485-11488 CC denotes and
T4240 11489-11497 NN denotes tartrate
T4242 11497-11498 HYPH denotes -
T4241 11498-11507 JJ denotes resistant
T4244 11508-11511 NN denotes ALP
T4245 11512-11513 -LRB- denotes (
T4247 11513-11521 NN denotes tartrate
T4249 11521-11522 HYPH denotes -
T4248 11522-11531 JJ denotes resistant
T4251 11532-11536 NN denotes acid
T4250 11537-11548 NN denotes phosphatase
T4252 11549-11550 -LRB- denotes [
T4253 11550-11554 NN denotes TRAP
T4254 11554-11555 -RRB- denotes ]
T4255 11555-11556 : denotes ;
T4256 11557-11560 JJ denotes red
T4246 11561-11566 NN denotes stain
T4257 11566-11567 -RRB- denotes )
T4243 11568-11576 NN denotes activity
T4258 11577-11579 IN denotes as
T4259 11580-11581 DT denotes a
T4260 11582-11588 NN denotes marker
T4261 11589-11592 IN denotes for
T4262 11593-11604 NNS denotes osteoclasts
T4263 11604-11605 . denotes .
T4264 11605-11725 sentence denotes Little difference was seen between Sam68+/+ (Figure 5A and 5B) and Sam68−/− (Figure 5C and 5D) mice at 4 months of age.
T4265 11606-11612 JJ denotes Little
T4266 11613-11623 NN denotes difference
T4268 11624-11627 VBD denotes was
T4267 11628-11632 VBN denotes seen
T4269 11633-11640 IN denotes between
T4270 11641-11646 NN denotes Sam68
T4272 11646-11647 SYM denotes +
T4273 11647-11648 HYPH denotes /
T4274 11648-11649 SYM denotes +
T4275 11650-11651 -LRB- denotes (
T4277 11651-11657 NN denotes Figure
T4276 11658-11660 NN denotes 5A
T4278 11661-11664 CC denotes and
T4279 11665-11667 NN denotes 5B
T4280 11667-11668 -RRB- denotes )
T4281 11669-11672 CC denotes and
T4282 11673-11678 NN denotes Sam68
T4283 11678-11679 SYM denotes −
T4284 11679-11680 HYPH denotes /
T4285 11680-11681 SYM denotes −
T4286 11682-11683 -LRB- denotes (
T4288 11683-11689 NN denotes Figure
T4287 11690-11692 NN denotes 5C
T4289 11693-11696 CC denotes and
T4290 11697-11699 NN denotes 5D
T4291 11699-11700 -RRB- denotes )
T4271 11701-11705 NNS denotes mice
T4292 11706-11708 IN denotes at
T4293 11709-11710 CD denotes 4
T4294 11711-11717 NNS denotes months
T4295 11718-11720 IN denotes of
T4296 11721-11724 NN denotes age
T4297 11724-11725 . denotes .
T4298 11725-11893 sentence denotes ALP- and TRAP-positive cells were reduced in the 12-month-old Sam68+/+ mice (Figure 5E and 5F) and remained unchanged in 12-month-old Sam68−/− mice (Figure 5G and 5H).
T4299 11726-11729 NN denotes ALP
T4301 11729-11730 HYPH denotes -
T4302 11731-11734 CC denotes and
T4303 11735-11739 NN denotes TRAP
T4304 11739-11740 HYPH denotes -
T4300 11740-11748 JJ denotes positive
T4305 11749-11754 NNS denotes cells
T4307 11755-11759 VBD denotes were
T4306 11760-11767 VBN denotes reduced
T4308 11768-11770 IN denotes in
T4309 11771-11774 DT denotes the
T4311 11775-11777 CD denotes 12
T4313 11777-11778 HYPH denotes -
T4312 11778-11783 NN denotes month
T4315 11783-11784 HYPH denotes -
T4314 11784-11787 JJ denotes old
T4316 11788-11793 NN denotes Sam68
T4317 11793-11794 SYM denotes +
T4318 11794-11795 HYPH denotes /
T4319 11795-11796 SYM denotes +
T4310 11797-11801 NNS denotes mice
T4320 11802-11803 -LRB- denotes (
T4322 11803-11809 NN denotes Figure
T4321 11810-11812 NN denotes 5E
T4323 11813-11816 CC denotes and
T4324 11817-11819 NN denotes 5F
T4325 11819-11820 -RRB- denotes )
T4326 11821-11824 CC denotes and
T4327 11825-11833 VBD denotes remained
T4328 11834-11843 JJ denotes unchanged
T4329 11844-11846 IN denotes in
T4330 11847-11849 CD denotes 12
T4332 11849-11850 HYPH denotes -
T4331 11850-11855 NN denotes month
T4334 11855-11856 HYPH denotes -
T4333 11856-11859 JJ denotes old
T4336 11860-11865 NN denotes Sam68
T4337 11865-11866 SYM denotes −
T4338 11866-11867 HYPH denotes /
T4339 11867-11868 SYM denotes −
T4335 11869-11873 NNS denotes mice
T4340 11874-11875 -LRB- denotes (
T4342 11875-11881 NN denotes Figure
T4341 11882-11884 NN denotes 5G
T4343 11885-11888 CC denotes and
T4344 11889-11891 NN denotes 5H
T4345 11891-11892 -RRB- denotes )
T4346 11892-11893 . denotes .
T4347 11893-12131 sentence denotes The reduction in both osteoblast and osteoclast activity in the 12-month-old Sam68+/+ mice argued against bone being lost primarily due to a relative increase in osteoclast over osteoblast activity, as seen in high turnover disease [49].
T4348 11894-11897 DT denotes The
T4349 11898-11907 NN denotes reduction
T4351 11908-11910 IN denotes in
T4352 11911-11915 CC denotes both
T4353 11916-11926 NN denotes osteoblast
T4355 11927-11930 CC denotes and
T4356 11931-11941 NN denotes osteoclast
T4354 11942-11950 NN denotes activity
T4357 11951-11953 IN denotes in
T4358 11954-11957 DT denotes the
T4360 11958-11960 CD denotes 12
T4362 11960-11961 HYPH denotes -
T4361 11961-11966 NN denotes month
T4364 11966-11967 HYPH denotes -
T4363 11967-11970 JJ denotes old
T4365 11971-11976 NN denotes Sam68
T4366 11976-11977 SYM denotes +
T4367 11977-11978 HYPH denotes /
T4368 11978-11979 SYM denotes +
T4359 11980-11984 NNS denotes mice
T4350 11985-11991 VBD denotes argued
T4369 11992-11999 IN denotes against
T4370 12000-12004 NN denotes bone
T4372 12005-12010 VBG denotes being
T4371 12011-12015 VBN denotes lost
T4373 12016-12025 RB denotes primarily
T4374 12026-12029 IN denotes due
T4375 12030-12032 IN denotes to
T4376 12033-12034 DT denotes a
T4378 12035-12043 JJ denotes relative
T4377 12044-12052 NN denotes increase
T4379 12053-12055 IN denotes in
T4380 12056-12066 NN denotes osteoclast
T4381 12067-12071 IN denotes over
T4382 12072-12082 NN denotes osteoblast
T4383 12083-12091 NN denotes activity
T4384 12091-12093 , denotes ,
T4385 12093-12095 IN denotes as
T4386 12096-12100 VBN denotes seen
T4387 12101-12103 IN denotes in
T4388 12104-12108 JJ denotes high
T4389 12109-12117 NN denotes turnover
T4390 12118-12125 NN denotes disease
T4391 12126-12127 -LRB- denotes [
T4392 12127-12129 CD denotes 49
T4393 12129-12130 -RRB- denotes ]
T4394 12130-12131 . denotes .
T4395 12131-12885 sentence denotes Figure 5 Histologic Analysis of Undecalcified Bone from Sam68+/+ and Sam68−/− Mice Sections of tibia fixed in 4% paraformaldehyde and embedded in plastic were stained for ALP (A–C, E–G) activity to identify osteoblasts or for TRAP (B–D, F–H) activity to identify osteoclasts. Staining patterns were similar in 4-month-old Sam68+/+ (A and B), 4-month-old Sam68−/− (C and D), and 12-month-old Sam68−/− (G and H) mice compared with 12-month-old Sam68+/+ mice (E and F). Magnification at source, left panels ×10 and right panels ×40. Micrographs are representative of those taken from five to seven sections in each group of animals. Histomorphometric analyses [50] of the long bones (Table 3) corroborated the radiologic evidence of age-related bone loss.
T4396 12763-12780 JJ denotes Histomorphometric
T4397 12781-12789 NNS denotes analyses
T4399 12790-12791 -LRB- denotes [
T4400 12791-12793 CD denotes 50
T4401 12793-12794 -RRB- denotes ]
T4402 12795-12797 IN denotes of
T4403 12798-12801 DT denotes the
T4405 12802-12806 JJ denotes long
T4404 12807-12812 NNS denotes bones
T4406 12813-12814 -LRB- denotes (
T4407 12814-12819 NN denotes Table
T4408 12820-12821 CD denotes 3
T4409 12821-12822 -RRB- denotes )
T4398 12823-12835 VBD denotes corroborated
T4410 12836-12839 DT denotes the
T4412 12840-12850 JJ denotes radiologic
T4411 12851-12859 NN denotes evidence
T4413 12860-12862 IN denotes of
T4414 12863-12866 NN denotes age
T4416 12866-12867 HYPH denotes -
T4415 12867-12874 VBN denotes related
T4418 12875-12879 NN denotes bone
T4417 12880-12884 NN denotes loss
T4419 12884-12885 . denotes .
T4420 12885-13072 sentence denotes Bone volume (BV/TV), newly formed osteoid (OV/TV), and mineral apposition rate (MAR) were all significantly reduced in 12-month-old Sam68+/+ mice compared with 4-month-old Sam68+/+ mice.
T4421 12886-12890 NN denotes Bone
T4422 12891-12897 NN denotes volume
T4424 12898-12899 -LRB- denotes (
T4425 12899-12901 NN denotes BV
T4427 12901-12902 HYPH denotes /
T4426 12902-12904 NN denotes TV
T4428 12904-12905 -RRB- denotes )
T4429 12905-12907 , denotes ,
T4430 12907-12912 RB denotes newly
T4431 12913-12919 VBN denotes formed
T4432 12920-12927 JJ denotes osteoid
T4433 12928-12929 -LRB- denotes (
T4434 12929-12931 NN denotes OV
T4436 12931-12932 HYPH denotes /
T4435 12932-12934 NN denotes TV
T4437 12934-12935 -RRB- denotes )
T4438 12935-12937 , denotes ,
T4439 12937-12940 CC denotes and
T4440 12941-12948 NN denotes mineral
T4442 12949-12959 NN denotes apposition
T4441 12960-12964 NN denotes rate
T4443 12965-12966 -LRB- denotes (
T4444 12966-12969 NN denotes MAR
T4445 12969-12970 -RRB- denotes )
T4446 12971-12975 VBD denotes were
T4447 12976-12979 DT denotes all
T4448 12980-12993 RB denotes significantly
T4423 12994-13001 VBN denotes reduced
T4449 13002-13004 IN denotes in
T4450 13005-13007 CD denotes 12
T4452 13007-13008 HYPH denotes -
T4451 13008-13013 NN denotes month
T4454 13013-13014 HYPH denotes -
T4453 13014-13017 JJ denotes old
T4456 13018-13023 NN denotes Sam68
T4457 13023-13024 SYM denotes +
T4458 13024-13025 HYPH denotes /
T4459 13025-13026 SYM denotes +
T4455 13027-13031 NNS denotes mice
T4460 13032-13040 VBN denotes compared
T4461 13041-13045 IN denotes with
T4462 13046-13047 CD denotes 4
T4464 13047-13048 HYPH denotes -
T4463 13048-13053 NN denotes month
T4466 13053-13054 HYPH denotes -
T4465 13054-13057 JJ denotes old
T4468 13058-13063 NN denotes Sam68
T4469 13063-13064 SYM denotes +
T4470 13064-13065 HYPH denotes /
T4471 13065-13066 SYM denotes +
T4467 13067-13071 NNS denotes mice
T4472 13071-13072 . denotes .
T4473 13072-13255 sentence denotes These reductions were associated with a significant increase in marrow fat (FV/TV) and decreased numbers of osteoblasts (nOB/TV) and osteoclasts (nOC/TV) per tissue volume (Table 3).
T4474 13073-13078 DT denotes These
T4475 13079-13089 NNS denotes reductions
T4477 13090-13094 VBD denotes were
T4476 13095-13105 VBN denotes associated
T4478 13106-13110 IN denotes with
T4479 13111-13112 DT denotes a
T4481 13113-13124 JJ denotes significant
T4480 13125-13133 NN denotes increase
T4482 13134-13136 IN denotes in
T4483 13137-13143 NN denotes marrow
T4484 13144-13147 NN denotes fat
T4485 13148-13149 -LRB- denotes (
T4487 13149-13151 NN denotes FV
T4488 13151-13152 HYPH denotes /
T4486 13152-13154 NN denotes TV
T4489 13154-13155 -RRB- denotes )
T4490 13156-13159 CC denotes and
T4491 13160-13169 VBN denotes decreased
T4492 13170-13177 NNS denotes numbers
T4493 13178-13180 IN denotes of
T4494 13181-13192 NNS denotes osteoblasts
T4495 13193-13194 -LRB- denotes (
T4497 13194-13197 NN denotes nOB
T4498 13197-13198 HYPH denotes /
T4496 13198-13200 NN denotes TV
T4499 13200-13201 -RRB- denotes )
T4500 13202-13205 CC denotes and
T4501 13206-13217 NNS denotes osteoclasts
T4502 13218-13219 -LRB- denotes (
T4504 13219-13222 NN denotes nOC
T4505 13222-13223 HYPH denotes /
T4503 13223-13225 NN denotes TV
T4506 13225-13226 -RRB- denotes )
T4507 13227-13230 IN denotes per
T4508 13231-13237 NN denotes tissue
T4509 13238-13244 NN denotes volume
T4510 13245-13246 -LRB- denotes (
T4511 13246-13251 NN denotes Table
T4512 13252-13253 CD denotes 3
T4513 13253-13254 -RRB- denotes )
T4514 13254-13255 . denotes .
T4515 13255-13454 sentence denotes These results were in contrast to those of the 12-month-old Sam68−/− mice in which all histomorphometric parameters, including marrow fat, resembled those of young mice of either genotype (Table 3).
T4516 13256-13261 DT denotes These
T4517 13262-13269 NNS denotes results
T4518 13270-13274 VBD denotes were
T4519 13275-13277 IN denotes in
T4520 13278-13286 NN denotes contrast
T4521 13287-13289 IN denotes to
T4522 13290-13295 DT denotes those
T4523 13296-13298 IN denotes of
T4524 13299-13302 DT denotes the
T4526 13303-13305 CD denotes 12
T4528 13305-13306 HYPH denotes -
T4527 13306-13311 NN denotes month
T4530 13311-13312 HYPH denotes -
T4529 13312-13315 JJ denotes old
T4531 13316-13321 NN denotes Sam68
T4532 13321-13322 SYM denotes −
T4533 13322-13323 HYPH denotes /
T4534 13323-13324 SYM denotes −
T4525 13325-13329 NNS denotes mice
T4535 13330-13332 IN denotes in
T4537 13333-13338 WDT denotes which
T4538 13339-13342 DT denotes all
T4540 13343-13360 JJ denotes histomorphometric
T4539 13361-13371 NNS denotes parameters
T4541 13371-13373 , denotes ,
T4542 13373-13382 VBG denotes including
T4543 13383-13389 NN denotes marrow
T4544 13390-13393 NN denotes fat
T4545 13393-13395 , denotes ,
T4536 13395-13404 VBD denotes resembled
T4546 13405-13410 DT denotes those
T4547 13411-13413 IN denotes of
T4548 13414-13419 JJ denotes young
T4549 13420-13424 NNS denotes mice
T4550 13425-13427 IN denotes of
T4551 13428-13434 CC denotes either
T4552 13435-13443 NN denotes genotype
T4553 13444-13445 -LRB- denotes (
T4554 13445-13450 NN denotes Table
T4555 13451-13452 CD denotes 3
T4556 13452-13453 -RRB- denotes )
T4557 13453-13454 . denotes .
T4558 13454-13661 sentence denotes When cells were expressed as a function of the bone perimeter (nOB/BP, nOC/BP), there were no statistical differences and the ratio of osteoblasts to osteoclasts was similar in all groups of mice (Table 3).
T4559 13455-13459 WRB denotes When
T4561 13460-13465 NNS denotes cells
T4562 13466-13470 VBD denotes were
T4560 13471-13480 VBN denotes expressed
T4564 13481-13483 IN denotes as
T4565 13484-13485 DT denotes a
T4566 13486-13494 NN denotes function
T4567 13495-13497 IN denotes of
T4568 13498-13501 DT denotes the
T4570 13502-13506 NN denotes bone
T4569 13507-13516 NN denotes perimeter
T4571 13517-13518 -LRB- denotes (
T4573 13518-13521 NN denotes nOB
T4574 13521-13522 HYPH denotes /
T4572 13522-13524 NN denotes BP
T4575 13524-13526 , denotes ,
T4576 13526-13529 NN denotes nOC
T4578 13529-13530 HYPH denotes /
T4577 13530-13532 NN denotes BP
T4579 13532-13533 -RRB- denotes )
T4580 13533-13535 , denotes ,
T4581 13535-13540 EX denotes there
T4563 13541-13545 VBD denotes were
T4582 13546-13548 DT denotes no
T4584 13549-13560 JJ denotes statistical
T4583 13561-13572 NNS denotes differences
T4585 13573-13576 CC denotes and
T4586 13577-13580 DT denotes the
T4587 13581-13586 NN denotes ratio
T4589 13587-13589 IN denotes of
T4590 13590-13601 NNS denotes osteoblasts
T4591 13602-13604 IN denotes to
T4592 13605-13616 NNS denotes osteoclasts
T4588 13617-13620 VBD denotes was
T4593 13621-13628 JJ denotes similar
T4594 13629-13631 IN denotes in
T4595 13632-13635 DT denotes all
T4596 13636-13642 NNS denotes groups
T4597 13643-13645 IN denotes of
T4598 13646-13650 NNS denotes mice
T4599 13651-13652 -LRB- denotes (
T4600 13652-13657 NN denotes Table
T4601 13658-13659 CD denotes 3
T4602 13659-13660 -RRB- denotes )
T4603 13660-13661 . denotes .
T4604 13661-13955 sentence denotes Table 3 Histomorphometric Analysis of Long Bones from 4- and 12-Month-Old Mice Circulating levels of serum C-telopeptide (sCTX; Roche Diagnostics, Mannheim, Germany) and ALP showed little difference among the groups, except for a small decrease in 12-month-old Sam68−/− mice and (Dataset S3).
T4605 13742-13753 VBG denotes Circulating
T4606 13754-13760 NNS denotes levels
T4608 13761-13763 IN denotes of
T4609 13764-13769 NN denotes serum
T4611 13770-13771 NN denotes C
T4612 13771-13772 HYPH denotes -
T4610 13772-13783 NN denotes telopeptide
T4613 13784-13785 -LRB- denotes (
T4615 13785-13789 NN denotes sCTX
T4616 13789-13790 : denotes ;
T4617 13791-13796 NNP denotes Roche
T4614 13797-13808 NNP denotes Diagnostics
T4618 13808-13810 , denotes ,
T4619 13810-13818 NNP denotes Mannheim
T4620 13818-13820 , denotes ,
T4621 13820-13827 NNP denotes Germany
T4622 13827-13828 -RRB- denotes )
T4623 13829-13832 CC denotes and
T4624 13833-13836 NN denotes ALP
T4607 13837-13843 VBD denotes showed
T4625 13844-13850 JJ denotes little
T4626 13851-13861 NN denotes difference
T4627 13862-13867 IN denotes among
T4628 13868-13871 DT denotes the
T4629 13872-13878 NNS denotes groups
T4630 13878-13880 , denotes ,
T4631 13880-13886 IN denotes except
T4632 13887-13890 IN denotes for
T4633 13891-13892 DT denotes a
T4635 13893-13898 JJ denotes small
T4634 13899-13907 NN denotes decrease
T4636 13908-13910 IN denotes in
T4637 13911-13913 CD denotes 12
T4639 13913-13914 HYPH denotes -
T4638 13914-13919 NN denotes month
T4641 13919-13920 HYPH denotes -
T4640 13920-13923 JJ denotes old
T4643 13924-13929 NN denotes Sam68
T4644 13929-13930 SYM denotes −
T4645 13930-13931 HYPH denotes /
T4646 13931-13932 SYM denotes −
T4642 13933-13937 NNS denotes mice
T4647 13938-13941 CC denotes and
T4648 13942-13943 -LRB- denotes (
T4650 13943-13950 NN denotes Dataset
T4649 13951-13953 NN denotes S3
T4651 13953-13954 -RRB- denotes )
T4652 13954-13955 . denotes .
T4653 13955-14111 sentence denotes Serum estrogen was significantly decreased in all 12-month-old mice regardless of genotype with a reciprocal increase in interleukin-6 levels (Dataset S3).
T4654 13956-13961 NN denotes Serum
T4655 13962-13970 NN denotes estrogen
T4657 13971-13974 VBD denotes was
T4658 13975-13988 RB denotes significantly
T4656 13989-13998 VBN denotes decreased
T4659 13999-14001 IN denotes in
T4660 14002-14005 DT denotes all
T4662 14006-14008 CD denotes 12
T4664 14008-14009 HYPH denotes -
T4663 14009-14014 NN denotes month
T4666 14014-14015 HYPH denotes -
T4665 14015-14018 JJ denotes old
T4661 14019-14023 NNS denotes mice
T4667 14024-14034 RB denotes regardless
T4668 14035-14037 IN denotes of
T4669 14038-14046 NN denotes genotype
T4670 14047-14051 IN denotes with
T4671 14052-14053 DT denotes a
T4673 14054-14064 JJ denotes reciprocal
T4672 14065-14073 NN denotes increase
T4674 14074-14076 IN denotes in
T4675 14077-14088 NN denotes interleukin
T4677 14088-14089 HYPH denotes -
T4678 14089-14090 CD denotes 6
T4676 14091-14097 NNS denotes levels
T4679 14098-14099 -LRB- denotes (
T4681 14099-14106 NN denotes Dataset
T4680 14107-14109 NN denotes S3
T4682 14109-14110 -RRB- denotes )
T4683 14110-14111 . denotes .
T4684 14111-14234 sentence denotes Given the involvement of leptin in regulating body and bone mass, serum leptin was measured in Sam68+/+ and Sam68−/− mice.
T4685 14112-14117 VBN denotes Given
T4687 14118-14121 DT denotes the
T4688 14122-14133 NN denotes involvement
T4689 14134-14136 IN denotes of
T4690 14137-14143 NN denotes leptin
T4691 14144-14146 IN denotes in
T4692 14147-14157 VBG denotes regulating
T4693 14158-14162 NN denotes body
T4695 14163-14166 CC denotes and
T4696 14167-14171 NN denotes bone
T4694 14172-14176 NN denotes mass
T4697 14176-14178 , denotes ,
T4698 14178-14183 NN denotes serum
T4699 14184-14190 NN denotes leptin
T4700 14191-14194 VBD denotes was
T4686 14195-14203 VBN denotes measured
T4701 14204-14206 IN denotes in
T4702 14207-14212 NN denotes Sam68
T4704 14212-14213 SYM denotes +
T4705 14213-14214 HYPH denotes /
T4706 14214-14215 SYM denotes +
T4707 14216-14219 CC denotes and
T4708 14220-14225 NN denotes Sam68
T4709 14225-14226 SYM denotes −
T4710 14226-14227 HYPH denotes /
T4711 14227-14228 SYM denotes −
T4703 14229-14233 NNS denotes mice
T4712 14233-14234 . denotes .
T4713 14234-14367 sentence denotes Twelve-month-old Sam68−/− mice had significantly lower levels than young and old Sam68+/+ mice and young Sam68−/− mice (Dataset S3).
T4714 14235-14241 CD denotes Twelve
T4716 14241-14242 HYPH denotes -
T4715 14242-14247 NN denotes month
T4718 14247-14248 HYPH denotes -
T4717 14248-14251 JJ denotes old
T4720 14252-14257 NN denotes Sam68
T4721 14257-14258 SYM denotes −
T4722 14258-14259 HYPH denotes /
T4723 14259-14260 SYM denotes −
T4719 14261-14265 NNS denotes mice
T4724 14266-14269 VBD denotes had
T4725 14270-14283 RB denotes significantly
T4726 14284-14289 JJR denotes lower
T4727 14290-14296 NNS denotes levels
T4728 14297-14301 IN denotes than
T4729 14302-14307 JJ denotes young
T4731 14308-14311 CC denotes and
T4732 14312-14315 JJ denotes old
T4733 14316-14321 NN denotes Sam68
T4734 14321-14322 SYM denotes +
T4735 14322-14323 HYPH denotes /
T4736 14323-14324 SYM denotes +
T4730 14325-14329 NNS denotes mice
T4737 14330-14333 CC denotes and
T4738 14334-14339 JJ denotes young
T4740 14340-14345 NN denotes Sam68
T4741 14345-14346 SYM denotes −
T4742 14346-14347 HYPH denotes /
T4743 14347-14348 SYM denotes −
T4739 14349-14353 NNS denotes mice
T4744 14354-14355 -LRB- denotes (
T4746 14355-14362 NN denotes Dataset
T4745 14363-14365 NN denotes S3
T4747 14365-14366 -RRB- denotes )
T4748 14366-14367 . denotes .
T4749 14367-14589 sentence denotes Taken together, these observations suggested that preservation of bone mass in the Sam68−/− mice was not due primarily to altered estrogen status but could have been influenced by differences in circulating leptin levels.
T4750 14368-14373 VBN denotes Taken
T4752 14374-14382 RB denotes together
T4753 14382-14384 , denotes ,
T4754 14384-14389 DT denotes these
T4755 14390-14402 NNS denotes observations
T4751 14403-14412 VBD denotes suggested
T4756 14413-14417 IN denotes that
T4758 14418-14430 NN denotes preservation
T4759 14431-14433 IN denotes of
T4760 14434-14438 NN denotes bone
T4761 14439-14443 NN denotes mass
T4762 14444-14446 IN denotes in
T4763 14447-14450 DT denotes the
T4765 14451-14456 NN denotes Sam68
T4766 14456-14457 SYM denotes −
T4767 14457-14458 HYPH denotes /
T4768 14458-14459 SYM denotes −
T4764 14460-14464 NNS denotes mice
T4757 14465-14468 VBD denotes was
T4769 14469-14472 RB denotes not
T4770 14473-14476 IN denotes due
T4771 14477-14486 RB denotes primarily
T4772 14487-14489 IN denotes to
T4773 14490-14497 VBN denotes altered
T4775 14498-14506 NN denotes estrogen
T4774 14507-14513 NN denotes status
T4776 14514-14517 CC denotes but
T4777 14518-14523 MD denotes could
T4779 14524-14528 VB denotes have
T4780 14529-14533 VBN denotes been
T4778 14534-14544 VBN denotes influenced
T4781 14545-14547 IN denotes by
T4782 14548-14559 NNS denotes differences
T4783 14560-14562 IN denotes in
T4784 14563-14574 VBG denotes circulating
T4786 14575-14581 NN denotes leptin
T4785 14582-14588 NNS denotes levels
T4787 14588-14589 . denotes .
T4956 14591-14601 NN denotes Osteoblast
T4958 14601-14603 , denotes ,
T4959 14603-14606 CC denotes but
T4960 14607-14610 RB denotes Not
T4961 14611-14621 NN denotes Osteoclast
T4962 14621-14623 , denotes ,
T4957 14623-14631 NN denotes Activity
T4964 14632-14634 VBZ denotes Is
T4963 14635-14642 VBN denotes Altered
T4965 14643-14645 IN denotes in
T4966 14646-14651 NN denotes Sam68
T4968 14651-14652 SYM denotes −
T4969 14652-14653 HYPH denotes /
T4970 14653-14654 SYM denotes −
T4967 14655-14659 NNS denotes Mice
T4971 14660-14662 FW denotes Ex
T4972 14663-14667 FW denotes Vivo
T4973 14667-14846 sentence denotes Maintenance of bone mass during the adult remodeling cycle is dependent on the coupling of osteoblast to osteoclast activity, such that there is no net gain or loss of bone [49].
T4974 14668-14679 NN denotes Maintenance
T4976 14680-14682 IN denotes of
T4977 14683-14687 NN denotes bone
T4978 14688-14692 NN denotes mass
T4979 14693-14699 IN denotes during
T4980 14700-14703 DT denotes the
T4982 14704-14709 JJ denotes adult
T4983 14710-14720 NN denotes remodeling
T4981 14721-14726 NN denotes cycle
T4975 14727-14729 VBZ denotes is
T4984 14730-14739 JJ denotes dependent
T4985 14740-14742 IN denotes on
T4986 14743-14746 DT denotes the
T4987 14747-14755 NN denotes coupling
T4988 14756-14758 IN denotes of
T4989 14759-14769 NN denotes osteoblast
T4990 14770-14772 IN denotes to
T4991 14773-14783 NN denotes osteoclast
T4992 14784-14792 NN denotes activity
T4993 14792-14794 , denotes ,
T4994 14794-14798 JJ denotes such
T4996 14799-14803 IN denotes that
T4997 14804-14809 EX denotes there
T4995 14810-14812 VBZ denotes is
T4998 14813-14815 DT denotes no
T5000 14816-14819 JJ denotes net
T4999 14820-14824 NN denotes gain
T5001 14825-14827 CC denotes or
T5002 14828-14832 NN denotes loss
T5003 14833-14835 IN denotes of
T5004 14836-14840 NN denotes bone
T5005 14841-14842 -LRB- denotes [
T5006 14842-14844 CD denotes 49
T5007 14844-14845 -RRB- denotes ]
T5008 14845-14846 . denotes .
T5009 14846-15047 sentence denotes To further explore the age-related advantage of Sam68−/− mice with respect to bone preservation, we examined the functional activity of Sam68−/− osteoblasts and osteoclasts ex vivo (Figure 6A and 6B).
T5010 14847-14849 TO denotes To
T5012 14850-14857 RB denotes further
T5011 14858-14865 VB denotes explore
T5014 14866-14869 DT denotes the
T5016 14870-14873 NN denotes age
T5018 14873-14874 HYPH denotes -
T5017 14874-14881 VBN denotes related
T5015 14882-14891 NN denotes advantage
T5019 14892-14894 IN denotes of
T5020 14895-14900 NN denotes Sam68
T5022 14900-14901 SYM denotes −
T5023 14901-14902 HYPH denotes /
T5024 14902-14903 SYM denotes −
T5021 14904-14908 NNS denotes mice
T5025 14909-14913 IN denotes with
T5026 14914-14921 NN denotes respect
T5027 14922-14924 IN denotes to
T5028 14925-14929 NN denotes bone
T5029 14930-14942 NN denotes preservation
T5030 14942-14944 , denotes ,
T5031 14944-14946 PRP denotes we
T5013 14947-14955 VBD denotes examined
T5032 14956-14959 DT denotes the
T5034 14960-14970 JJ denotes functional
T5033 14971-14979 NN denotes activity
T5035 14980-14982 IN denotes of
T5036 14983-14988 NN denotes Sam68
T5037 14988-14989 SYM denotes −
T5038 14989-14990 HYPH denotes /
T5039 14990-14991 SYM denotes −
T5040 14992-15003 NNS denotes osteoblasts
T5041 15004-15007 CC denotes and
T5042 15008-15019 NNS denotes osteoclasts
T5043 15020-15022 FW denotes ex
T5044 15023-15027 FW denotes vivo
T5045 15028-15029 -LRB- denotes (
T5047 15029-15035 NN denotes Figure
T5046 15036-15038 NN denotes 6A
T5048 15039-15042 CC denotes and
T5049 15043-15045 NN denotes 6B
T5050 15045-15046 -RRB- denotes )
T5051 15046-15047 . denotes .
T5052 15047-15470 sentence denotes Cultures of bone marrow stromal cells harvested from 4-week-old Sam68−/− mice and maintained for 18 days in osteoblast differentiation medium demonstrated more intense staining for ALP at 6 days (unpublished data) and 18 days (Figure 6A), and more mineralized nodules at 18 days, than the Sam68+/+ mice, even though similar amounts of fibroblast colony-forming units (CFU-F) were observed (Figure 6A and unpublished data).
T5053 15048-15056 NNS denotes Cultures
T5055 15057-15059 IN denotes of
T5056 15060-15064 NN denotes bone
T5057 15065-15071 NN denotes marrow
T5059 15072-15079 JJ denotes stromal
T5058 15080-15085 NNS denotes cells
T5060 15086-15095 VBN denotes harvested
T5061 15096-15100 IN denotes from
T5062 15101-15102 CD denotes 4
T5064 15102-15103 HYPH denotes -
T5063 15103-15107 NN denotes week
T5066 15107-15108 HYPH denotes -
T5065 15108-15111 JJ denotes old
T5068 15112-15117 NN denotes Sam68
T5069 15117-15118 SYM denotes −
T5070 15118-15119 HYPH denotes /
T5071 15119-15120 SYM denotes −
T5067 15121-15125 NNS denotes mice
T5072 15126-15129 CC denotes and
T5073 15130-15140 VBN denotes maintained
T5074 15141-15144 IN denotes for
T5075 15145-15147 CD denotes 18
T5076 15148-15152 NNS denotes days
T5077 15153-15155 IN denotes in
T5078 15156-15166 NN denotes osteoblast
T5079 15167-15182 NN denotes differentiation
T5080 15183-15189 NN denotes medium
T5054 15190-15202 VBD denotes demonstrated
T5081 15203-15207 RBR denotes more
T5082 15208-15215 JJ denotes intense
T5083 15216-15224 NN denotes staining
T5084 15225-15228 IN denotes for
T5085 15229-15232 NN denotes ALP
T5086 15233-15235 IN denotes at
T5087 15236-15237 CD denotes 6
T5088 15238-15242 NNS denotes days
T5089 15243-15244 -LRB- denotes (
T5091 15244-15255 JJ denotes unpublished
T5090 15256-15260 NNS denotes data
T5092 15260-15261 -RRB- denotes )
T5093 15262-15265 CC denotes and
T5094 15266-15268 CD denotes 18
T5095 15269-15273 NNS denotes days
T5096 15274-15275 -LRB- denotes (
T5098 15275-15281 NN denotes Figure
T5097 15282-15284 NN denotes 6A
T5099 15284-15285 NN denotes )
T5100 15285-15287 NN denotes ,
T5101 15287-15290 CC denotes and
T5102 15291-15295 JJR denotes more
T5104 15296-15307 VBN denotes mineralized
T5103 15308-15315 NNS denotes nodules
T5105 15316-15318 IN denotes at
T5106 15319-15321 CD denotes 18
T5107 15322-15326 NNS denotes days
T5108 15326-15328 NN denotes ,
T5109 15328-15332 IN denotes than
T5110 15333-15336 DT denotes the
T5112 15337-15342 NN denotes Sam68
T5113 15342-15343 SYM denotes +
T5114 15343-15344 HYPH denotes /
T5115 15344-15345 SYM denotes +
T5111 15346-15350 NNS denotes mice
T5116 15350-15352 NN denotes ,
T5117 15352-15356 RB denotes even
T5119 15357-15363 IN denotes though
T5120 15364-15371 JJ denotes similar
T5121 15372-15379 NNS denotes amounts
T5122 15380-15382 IN denotes of
T5123 15383-15393 NN denotes fibroblast
T5125 15394-15400 NN denotes colony
T5127 15400-15401 HYPH denotes -
T5126 15401-15408 VBG denotes forming
T5124 15409-15414 NNS denotes units
T5128 15415-15416 -LRB- denotes (
T5129 15416-15419 NN denotes CFU
T5131 15419-15420 HYPH denotes -
T5130 15420-15421 NN denotes F
T5132 15421-15422 -RRB- denotes )
T5133 15423-15427 VBD denotes were
T5118 15428-15436 VBN denotes observed
T5134 15437-15438 -LRB- denotes (
T5136 15438-15444 NN denotes Figure
T5135 15445-15447 NN denotes 6A
T5137 15448-15451 CC denotes and
T5138 15452-15463 JJ denotes unpublished
T5139 15464-15468 NNS denotes data
T5140 15468-15469 -RRB- denotes )
T5141 15469-15470 . denotes .
T5142 15470-15721 sentence denotes RT-PCR analysis of molecular markers of osteoblast differentiation (Dataset S2) revealed similar increases over time in RNA from Sam68+/+ and Sam68−/− mice, although type I collagen appeared to be up-regulated at both timepoints in the Sam68−/− mice.
T5143 15471-15473 NN denotes RT
T5145 15473-15474 HYPH denotes -
T5144 15474-15477 NN denotes PCR
T5146 15478-15486 NN denotes analysis
T5148 15487-15489 IN denotes of
T5149 15490-15499 JJ denotes molecular
T5150 15500-15507 NNS denotes markers
T5151 15508-15510 IN denotes of
T5152 15511-15521 NN denotes osteoblast
T5153 15522-15537 NN denotes differentiation
T5154 15538-15539 -LRB- denotes (
T5156 15539-15546 NN denotes Dataset
T5155 15547-15549 NN denotes S2
T5157 15549-15550 -RRB- denotes )
T5147 15551-15559 VBD denotes revealed
T5158 15560-15567 JJ denotes similar
T5159 15568-15577 NNS denotes increases
T5160 15578-15582 IN denotes over
T5161 15583-15587 NN denotes time
T5162 15588-15590 IN denotes in
T5163 15591-15594 NN denotes RNA
T5164 15595-15599 IN denotes from
T5165 15600-15605 NN denotes Sam68
T5167 15605-15606 SYM denotes +
T5168 15606-15607 HYPH denotes /
T5169 15607-15608 SYM denotes +
T5170 15609-15612 CC denotes and
T5171 15613-15618 NN denotes Sam68
T5172 15618-15619 SYM denotes −
T5173 15619-15620 HYPH denotes /
T5174 15620-15621 SYM denotes −
T5166 15622-15626 NNS denotes mice
T5175 15626-15628 , denotes ,
T5176 15628-15636 IN denotes although
T5178 15637-15641 NN denotes type
T5180 15642-15643 CD denotes I
T5179 15644-15652 NN denotes collagen
T5177 15653-15661 VBD denotes appeared
T5181 15662-15664 TO denotes to
T5183 15665-15667 VB denotes be
T5184 15668-15670 RB denotes up
T5185 15670-15671 HYPH denotes -
T5182 15671-15680 VBN denotes regulated
T5186 15681-15683 IN denotes at
T5187 15684-15688 DT denotes both
T5188 15689-15699 NNS denotes timepoints
T5189 15700-15702 IN denotes in
T5190 15703-15706 DT denotes the
T5192 15707-15712 NN denotes Sam68
T5193 15712-15713 SYM denotes −
T5194 15713-15714 HYPH denotes /
T5195 15714-15715 SYM denotes −
T5191 15716-15720 NNS denotes mice
T5196 15720-15721 . denotes .
T5197 15721-16001 sentence denotes Short-term cultures of mature osteoclasts released from the crushed long bones of Sam68+/+ and Sam68−/− mice stained equally well for TRAP and excavated approximately equal numbers of pits of equal size in dentin slices, as visualized by scanning electron microscopy (Figure 6B).
T5198 15722-15727 JJ denotes Short
T5200 15727-15728 HYPH denotes -
T5199 15728-15732 NN denotes term
T5201 15733-15741 NNS denotes cultures
T5203 15742-15744 IN denotes of
T5204 15745-15751 JJ denotes mature
T5205 15752-15763 NNS denotes osteoclasts
T5206 15764-15772 VBN denotes released
T5207 15773-15777 IN denotes from
T5208 15778-15781 DT denotes the
T5210 15782-15789 JJ denotes crushed
T5211 15790-15794 JJ denotes long
T5209 15795-15800 NNS denotes bones
T5212 15801-15803 IN denotes of
T5213 15804-15809 NN denotes Sam68
T5215 15809-15810 SYM denotes +
T5216 15810-15811 HYPH denotes /
T5217 15811-15812 SYM denotes +
T5218 15813-15816 CC denotes and
T5219 15817-15822 NN denotes Sam68
T5220 15822-15823 SYM denotes −
T5221 15823-15824 HYPH denotes /
T5222 15824-15825 SYM denotes −
T5214 15826-15830 NNS denotes mice
T5202 15831-15838 VBD denotes stained
T5223 15839-15846 RB denotes equally
T5224 15847-15851 RB denotes well
T5225 15852-15855 IN denotes for
T5226 15856-15860 NN denotes TRAP
T5227 15861-15864 CC denotes and
T5228 15865-15874 VBD denotes excavated
T5229 15875-15888 RB denotes approximately
T5230 15889-15894 JJ denotes equal
T5231 15895-15902 NNS denotes numbers
T5232 15903-15905 IN denotes of
T5233 15906-15910 NNS denotes pits
T5234 15911-15913 IN denotes of
T5235 15914-15919 JJ denotes equal
T5236 15920-15924 NN denotes size
T5237 15925-15927 IN denotes in
T5238 15928-15934 NN denotes dentin
T5239 15935-15941 NNS denotes slices
T5240 15941-15943 , denotes ,
T5241 15943-15945 IN denotes as
T5242 15946-15956 VBN denotes visualized
T5243 15957-15959 IN denotes by
T5244 15960-15968 VBG denotes scanning
T5245 15969-15977 NN denotes electron
T5246 15978-15988 NN denotes microscopy
T5247 15989-15990 -LRB- denotes (
T5249 15990-15996 NN denotes Figure
T5248 15997-15999 NN denotes 6B
T5250 15999-16000 -RRB- denotes )
T5251 16000-16001 . denotes .
T5252 16001-16128 sentence denotes These findings suggest that the osteoclasts may not be the primary defect in Sam68−/− mice, as they are in Src null mice [48].
T5253 16002-16007 DT denotes These
T5254 16008-16016 NNS denotes findings
T5255 16017-16024 VBP denotes suggest
T5256 16025-16029 IN denotes that
T5258 16030-16033 DT denotes the
T5259 16034-16045 NNS denotes osteoclasts
T5260 16046-16049 MD denotes may
T5261 16050-16053 RB denotes not
T5257 16054-16056 VB denotes be
T5262 16057-16060 DT denotes the
T5264 16061-16068 JJ denotes primary
T5263 16069-16075 NN denotes defect
T5265 16076-16078 IN denotes in
T5266 16079-16084 NN denotes Sam68
T5268 16084-16085 SYM denotes −
T5269 16085-16086 HYPH denotes /
T5270 16086-16087 SYM denotes −
T5267 16088-16092 NNS denotes mice
T5271 16092-16094 , denotes ,
T5272 16094-16096 IN denotes as
T5274 16097-16101 PRP denotes they
T5273 16102-16105 VBP denotes are
T5275 16106-16108 IN denotes in
T5276 16109-16112 NN denotes Src
T5278 16113-16117 JJ denotes null
T5277 16118-16122 NNS denotes mice
T5279 16123-16124 -LRB- denotes [
T5280 16124-16126 CD denotes 48
T5281 16126-16127 -RRB- denotes ]
T5282 16127-16128 . denotes .
T5689 17194-17197 DT denotes The
T5690 17198-17205 NN denotes Absence
T5692 17206-17208 IN denotes of
T5693 17209-17214 NN denotes Sam68
T5691 17215-17223 VBZ denotes Prevents
T5694 17224-17233 NN denotes Adipocyte
T5695 17234-17249 NN denotes Differentiation
T5696 17250-17253 CC denotes and
T5697 17254-17262 VBZ denotes Promotes
T5698 17263-17273 NN denotes Osteoblast
T5699 17274-17289 NN denotes Differentiation
T5700 17289-17500 sentence denotes It is well recognized that bone marrow stromal cells give rise to both osteoblasts and adipocytes and that age-related bone loss is accompanied by an increase in differentiation down the adipocyte lineage [22].
T5701 17290-17292 PRP denotes It
T5703 17293-17295 VBZ denotes is
T5704 17296-17300 RB denotes well
T5702 17301-17311 VBN denotes recognized
T5705 17312-17316 IN denotes that
T5707 17317-17321 NN denotes bone
T5708 17322-17328 NN denotes marrow
T5710 17329-17336 JJ denotes stromal
T5709 17337-17342 NNS denotes cells
T5706 17343-17347 VBP denotes give
T5711 17348-17352 NN denotes rise
T5712 17353-17355 IN denotes to
T5713 17356-17360 DT denotes both
T5714 17361-17372 NNS denotes osteoblasts
T5715 17373-17376 CC denotes and
T5716 17377-17387 NNS denotes adipocytes
T5717 17388-17391 CC denotes and
T5718 17392-17396 IN denotes that
T5720 17397-17400 NN denotes age
T5722 17400-17401 HYPH denotes -
T5721 17401-17408 VBN denotes related
T5724 17409-17413 NN denotes bone
T5723 17414-17418 NN denotes loss
T5725 17419-17421 VBZ denotes is
T5719 17422-17433 VBN denotes accompanied
T5726 17434-17436 IN denotes by
T5727 17437-17439 DT denotes an
T5728 17440-17448 NN denotes increase
T5729 17449-17451 IN denotes in
T5730 17452-17467 NN denotes differentiation
T5731 17468-17472 IN denotes down
T5732 17473-17476 DT denotes the
T5734 17477-17486 NN denotes adipocyte
T5733 17487-17494 NN denotes lineage
T5735 17495-17496 -LRB- denotes [
T5736 17496-17498 CD denotes 22
T5737 17498-17499 -RRB- denotes ]
T5738 17499-17500 . denotes .
T5739 17500-17644 sentence denotes Therefore, the loss of Sam68 could influence the bone marrow stromal cells to differentiate along the osteogenic versus the adipogenic pathway.
T5740 17501-17510 RB denotes Therefore
T5742 17510-17512 , denotes ,
T5743 17512-17515 DT denotes the
T5744 17516-17520 NN denotes loss
T5745 17521-17523 IN denotes of
T5746 17524-17529 NN denotes Sam68
T5747 17530-17535 MD denotes could
T5741 17536-17545 VB denotes influence
T5748 17546-17549 DT denotes the
T5750 17550-17554 NN denotes bone
T5751 17555-17561 NN denotes marrow
T5752 17562-17569 JJ denotes stromal
T5749 17570-17575 NNS denotes cells
T5753 17576-17578 TO denotes to
T5754 17579-17592 VB denotes differentiate
T5755 17593-17598 IN denotes along
T5756 17599-17602 DT denotes the
T5757 17603-17613 JJ denotes osteogenic
T5758 17614-17620 CC denotes versus
T5759 17621-17624 DT denotes the
T5760 17625-17635 JJ denotes adipogenic
T5761 17636-17643 NN denotes pathway
T5762 17643-17644 . denotes .
T5763 17644-17878 sentence denotes Alternatively, the loss of Sam68 could indirectly regulate bone mass as a general disturbance of neuroendocrine control as was shown when leptin and the sympathetic nervous system axis were shown to negatively regulate bone mass [8].
T5764 17645-17658 RB denotes Alternatively
T5766 17658-17660 , denotes ,
T5767 17660-17663 DT denotes the
T5768 17664-17668 NN denotes loss
T5769 17669-17671 IN denotes of
T5770 17672-17677 NN denotes Sam68
T5771 17678-17683 MD denotes could
T5772 17684-17694 RB denotes indirectly
T5765 17695-17703 VB denotes regulate
T5773 17704-17708 NN denotes bone
T5774 17709-17713 NN denotes mass
T5775 17714-17716 IN denotes as
T5776 17717-17718 DT denotes a
T5778 17719-17726 JJ denotes general
T5777 17727-17738 NN denotes disturbance
T5779 17739-17741 IN denotes of
T5780 17742-17756 JJ denotes neuroendocrine
T5781 17757-17764 NN denotes control
T5782 17765-17767 IN denotes as
T5784 17768-17771 VBD denotes was
T5783 17772-17777 VBN denotes shown
T5785 17778-17782 WRB denotes when
T5787 17783-17789 NN denotes leptin
T5788 17790-17793 CC denotes and
T5789 17794-17797 DT denotes the
T5791 17798-17809 JJ denotes sympathetic
T5792 17810-17817 JJ denotes nervous
T5793 17818-17824 NN denotes system
T5790 17825-17829 NN denotes axis
T5794 17830-17834 VBD denotes were
T5786 17835-17840 VBN denotes shown
T5795 17841-17843 TO denotes to
T5797 17844-17854 RB denotes negatively
T5796 17855-17863 VB denotes regulate
T5798 17864-17868 NN denotes bone
T5799 17869-17873 NN denotes mass
T5800 17874-17875 -LRB- denotes [
T5801 17875-17876 CD denotes 8
T5802 17876-17877 -RRB- denotes ]
T5803 17877-17878 . denotes .
T5804 17878-18028 sentence denotes To confirm a role for Sam68 in the regulation of adipocyte differentiation, we isolated primary MEFs from 14.5-day-old Sam68+/+ and Sam68−/− embryos.
T5805 17879-17881 TO denotes To
T5806 17882-17889 VB denotes confirm
T5808 17890-17891 DT denotes a
T5809 17892-17896 NN denotes role
T5810 17897-17900 IN denotes for
T5811 17901-17906 NN denotes Sam68
T5812 17907-17909 IN denotes in
T5813 17910-17913 DT denotes the
T5814 17914-17924 NN denotes regulation
T5815 17925-17927 IN denotes of
T5816 17928-17937 NN denotes adipocyte
T5817 17938-17953 NN denotes differentiation
T5818 17953-17955 , denotes ,
T5819 17955-17957 PRP denotes we
T5807 17958-17966 VBD denotes isolated
T5820 17967-17974 JJ denotes primary
T5821 17975-17979 NNS denotes MEFs
T5822 17980-17984 IN denotes from
T5823 17985-17989 CD denotes 14.5
T5825 17989-17990 HYPH denotes -
T5824 17990-17993 NN denotes day
T5827 17993-17994 HYPH denotes -
T5826 17994-17997 JJ denotes old
T5829 17998-18003 NN denotes Sam68
T5830 18003-18004 SYM denotes +
T5831 18004-18005 HYPH denotes /
T5832 18005-18006 SYM denotes +
T5833 18007-18010 CC denotes and
T5834 18011-18016 NN denotes Sam68
T5835 18016-18017 SYM denotes −
T5836 18017-18018 HYPH denotes /
T5837 18018-18019 SYM denotes −
T5828 18020-18027 NNS denotes embryos
T5838 18027-18028 . denotes .
T5839 18028-18170 sentence denotes The primary Sam68−/− MEFs were differentiated into adipocytes in vitro in culture medium containing 5 μM pioglitazone to induce adipogenesis.
T5840 18029-18032 DT denotes The
T5842 18033-18040 JJ denotes primary
T5843 18041-18046 NN denotes Sam68
T5844 18046-18047 SYM denotes −
T5845 18047-18048 HYPH denotes /
T5846 18048-18049 SYM denotes −
T5841 18050-18054 NNS denotes MEFs
T5848 18055-18059 VBD denotes were
T5847 18060-18074 VBN denotes differentiated
T5849 18075-18079 IN denotes into
T5850 18080-18090 NNS denotes adipocytes
T5851 18091-18093 FW denotes in
T5852 18094-18099 FW denotes vitro
T5853 18100-18102 IN denotes in
T5854 18103-18110 NN denotes culture
T5855 18111-18117 NN denotes medium
T5856 18118-18128 VBG denotes containing
T5857 18129-18130 CD denotes 5
T5858 18131-18133 NN denotes μM
T5859 18134-18146 NN denotes pioglitazone
T5860 18147-18149 TO denotes to
T5861 18150-18156 VB denotes induce
T5862 18157-18169 NN denotes adipogenesis
T5863 18169-18170 . denotes .
T5864 18170-18253 sentence denotes Cells at days 0, 4, 6, and 12 were stained with Oil red O to monitor adipogenesis.
T5865 18171-18176 NNS denotes Cells
T5867 18177-18179 IN denotes at
T5868 18180-18184 NNS denotes days
T5869 18185-18186 CD denotes 0
T5870 18186-18188 , denotes ,
T5871 18188-18189 CD denotes 4
T5872 18189-18191 , denotes ,
T5873 18191-18192 CD denotes 6
T5874 18192-18194 , denotes ,
T5875 18194-18197 CC denotes and
T5876 18198-18200 CD denotes 12
T5877 18201-18205 VBD denotes were
T5866 18206-18213 VBN denotes stained
T5878 18214-18218 IN denotes with
T5879 18219-18222 NN denotes Oil
T5881 18223-18226 NN denotes red
T5880 18227-18228 NN denotes O
T5882 18229-18231 TO denotes to
T5883 18232-18239 VB denotes monitor
T5884 18240-18252 NN denotes adipogenesis
T5885 18252-18253 . denotes .
T5886 18253-18439 sentence denotes Adipogenesis was more pronounced in the wild-type MEFs cultures than in the Sam68−/− MEFs, consistent with the positive role of endogenous Sam68 in adipocyte differentiation (Figure 7).
T5887 18254-18266 NN denotes Adipogenesis
T5888 18267-18270 VBD denotes was
T5889 18271-18275 RBR denotes more
T5890 18276-18286 JJ denotes pronounced
T5891 18287-18289 IN denotes in
T5892 18290-18293 DT denotes the
T5894 18294-18298 JJ denotes wild
T5896 18298-18299 HYPH denotes -
T5895 18299-18303 NN denotes type
T5897 18304-18308 NNS denotes MEFs
T5893 18309-18317 NNS denotes cultures
T5898 18318-18322 IN denotes than
T5899 18323-18325 IN denotes in
T5900 18326-18329 DT denotes the
T5902 18330-18335 NN denotes Sam68
T5903 18335-18336 SYM denotes −
T5904 18336-18337 HYPH denotes /
T5905 18337-18338 SYM denotes −
T5901 18339-18343 NNS denotes MEFs
T5906 18343-18345 , denotes ,
T5907 18345-18355 JJ denotes consistent
T5908 18356-18360 IN denotes with
T5909 18361-18364 DT denotes the
T5911 18365-18373 JJ denotes positive
T5910 18374-18378 NN denotes role
T5912 18379-18381 IN denotes of
T5913 18382-18392 JJ denotes endogenous
T5914 18393-18398 NN denotes Sam68
T5915 18399-18401 IN denotes in
T5916 18402-18411 NN denotes adipocyte
T5917 18412-18427 NN denotes differentiation
T5918 18428-18429 -LRB- denotes (
T5919 18429-18435 NN denotes Figure
T5920 18436-18437 CD denotes 7
T5921 18437-18438 -RRB- denotes )
T5922 18438-18439 . denotes .
T5923 18439-18660 sentence denotes The expression of key transcription factors including the PPARγ and KLF5 was impaired in Sam68−/− differentiated MEFs compared with Sam68+/+ MEFs, consistent with impaired adipogenesis in the absence of Sam68 (Figure 7).
T5924 18440-18443 DT denotes The
T5925 18444-18454 NN denotes expression
T5927 18455-18457 IN denotes of
T5928 18458-18461 JJ denotes key
T5930 18462-18475 NN denotes transcription
T5929 18476-18483 NNS denotes factors
T5931 18484-18493 VBG denotes including
T5932 18494-18497 DT denotes the
T5933 18498-18503 NN denotes PPARγ
T5934 18504-18507 CC denotes and
T5935 18508-18512 NN denotes KLF5
T5936 18513-18516 VBD denotes was
T5926 18517-18525 VBN denotes impaired
T5937 18526-18528 IN denotes in
T5938 18529-18534 NN denotes Sam68
T5940 18534-18535 SYM denotes −
T5941 18535-18536 HYPH denotes /
T5942 18536-18537 SYM denotes −
T5943 18538-18552 VBN denotes differentiated
T5939 18553-18557 NNS denotes MEFs
T5944 18558-18566 VBN denotes compared
T5945 18567-18571 IN denotes with
T5946 18572-18577 NN denotes Sam68
T5948 18577-18578 SYM denotes +
T5949 18578-18579 HYPH denotes /
T5950 18579-18580 SYM denotes +
T5947 18581-18585 NNS denotes MEFs
T5951 18585-18587 , denotes ,
T5952 18587-18597 JJ denotes consistent
T5953 18598-18602 IN denotes with
T5954 18603-18611 VBN denotes impaired
T5955 18612-18624 NN denotes adipogenesis
T5956 18625-18627 IN denotes in
T5957 18628-18631 DT denotes the
T5958 18632-18639 NN denotes absence
T5959 18640-18642 IN denotes of
T5960 18643-18648 NN denotes Sam68
T5961 18649-18650 -LRB- denotes (
T5962 18650-18656 NN denotes Figure
T5963 18657-18658 CD denotes 7
T5964 18658-18659 -RRB- denotes )
T5965 18659-18660 . denotes .
T5966 18660-18868 sentence denotes These data, together with data confirming a lean phenotype in Sam68−/− mice (N. Torabi and S. Richard, unpublished data), support the hypothesis that Sam68 modulates the differentiation of mesenchymal cells.
T5967 18661-18666 DT denotes These
T5968 18667-18671 NNS denotes data
T5970 18671-18673 , denotes ,
T5971 18673-18681 RB denotes together
T5972 18682-18686 IN denotes with
T5973 18687-18691 NNS denotes data
T5974 18692-18702 VBG denotes confirming
T5975 18703-18704 DT denotes a
T5977 18705-18709 JJ denotes lean
T5976 18710-18719 NN denotes phenotype
T5978 18720-18722 IN denotes in
T5979 18723-18728 NN denotes Sam68
T5981 18728-18729 SYM denotes −
T5982 18729-18730 HYPH denotes /
T5983 18730-18731 SYM denotes −
T5980 18732-18736 NNS denotes mice
T5984 18737-18738 -LRB- denotes (
T5985 18738-18740 NNP denotes N.
T5986 18741-18747 NNP denotes Torabi
T5987 18748-18751 CC denotes and
T5988 18752-18754 NNP denotes S.
T5989 18755-18762 NNP denotes Richard
T5990 18762-18764 , denotes ,
T5991 18764-18775 JJ denotes unpublished
T5992 18776-18780 NNS denotes data
T5993 18780-18781 -RRB- denotes )
T5994 18781-18783 , denotes ,
T5969 18783-18790 VBP denotes support
T5995 18791-18794 DT denotes the
T5996 18795-18805 NN denotes hypothesis
T5997 18806-18810 IN denotes that
T5999 18811-18816 NN denotes Sam68
T5998 18817-18826 VBZ denotes modulates
T6000 18827-18830 DT denotes the
T6001 18831-18846 NN denotes differentiation
T6002 18847-18849 IN denotes of
T6003 18850-18861 JJ denotes mesenchymal
T6004 18862-18867 NNS denotes cells
T6005 18867-18868 . denotes .
T6006 18868-19934 sentence denotes Figure 7 Ex Vivo Adipogenesis Analysis of Sam68−/− Mouse Embryonic Fibroblasts MEFs were isolated from mouse embryos at embryonic day 14.5. Equal number of MEFs from Sam68+/+ and Sam68−/− was plated on glass cover slips in 24 well-plates. Adipocyte differentiation was carried out at indicated times by the addition of complete media containing the pioglitazone. (A) Cultures were fixed in 4% paraformaldehyde and stained with Oil Red O to detect the fat droplets stored in adipocytes and photographed (top). The cell images were magnified ×10 and ×20 as indicated. (B) RT-PCR was carried out on total cellular RNA isolated after differentiation of the MEFs for day 0, 2, 4, 6, and 12. The DNA fragments were visualized on agarose gels stained with ethidium bromide. The expression of adipogenic markers C/EBPβ, C/EBPδ, PPARα, and KLF5 was examined as well as the expression of controls including Sam68, β-actin, and GAPDH. To further examine this phenotype in a cell autonomous system, we chose the embryonic mesenchymal multipotential progenitor cells C3H10T1/2.
T6007 19794-19796 TO denotes To
T6009 19797-19804 RB denotes further
T6008 19805-19812 VB denotes examine
T6011 19813-19817 DT denotes this
T6012 19818-19827 NN denotes phenotype
T6013 19828-19830 IN denotes in
T6014 19831-19832 DT denotes a
T6016 19833-19837 NN denotes cell
T6017 19838-19848 JJ denotes autonomous
T6015 19849-19855 NN denotes system
T6018 19855-19857 , denotes ,
T6019 19857-19859 PRP denotes we
T6010 19860-19865 VBD denotes chose
T6020 19866-19869 DT denotes the
T6022 19870-19879 JJ denotes embryonic
T6023 19880-19891 JJ denotes mesenchymal
T6024 19892-19906 JJ denotes multipotential
T6025 19907-19917 NN denotes progenitor
T6021 19918-19923 NNS denotes cells
T6026 19924-19931 NN denotes C3H10T1
T6027 19931-19932 HYPH denotes /
T6028 19932-19933 CD denotes 2
T6029 19933-19934 . denotes .
T6030 19934-20093 sentence denotes The addition of BMP-2 induced osteoblast differentiation, as evidenced by an approximately 400-fold increase in expression of the osteocalcin (OCN) gene [51].
T6031 19935-19938 DT denotes The
T6032 19939-19947 NN denotes addition
T6034 19948-19950 IN denotes of
T6035 19951-19954 NN denotes BMP
T6036 19954-19955 HYPH denotes -
T6037 19955-19956 CD denotes 2
T6033 19957-19964 VBD denotes induced
T6038 19965-19975 NN denotes osteoblast
T6039 19976-19991 NN denotes differentiation
T6040 19991-19993 , denotes ,
T6041 19993-19995 IN denotes as
T6042 19996-20005 VBN denotes evidenced
T6043 20006-20008 IN denotes by
T6044 20009-20011 DT denotes an
T6046 20012-20025 RB denotes approximately
T6047 20026-20034 JJ denotes 400-fold
T6045 20035-20043 NN denotes increase
T6048 20044-20046 IN denotes in
T6049 20047-20057 NN denotes expression
T6050 20058-20060 IN denotes of
T6051 20061-20064 DT denotes the
T6053 20065-20076 NN denotes osteocalcin
T6054 20077-20078 -LRB- denotes (
T6055 20078-20081 NN denotes OCN
T6056 20081-20082 -RRB- denotes )
T6052 20083-20087 NN denotes gene
T6057 20088-20089 -LRB- denotes [
T6058 20089-20091 CD denotes 51
T6059 20091-20092 -RRB- denotes ]
T6060 20092-20093 . denotes .
T6061 20093-20275 sentence denotes Populations of C3H10T1/2 cells stably transfected with either pSuper-retro (control) and pSuper-retro harboring a short hairpin against Sam68 (Sam68sh) were selected with puromycin.
T6062 20094-20105 NNS denotes Populations
T6064 20106-20108 IN denotes of
T6065 20109-20116 NN denotes C3H10T1
T6067 20116-20117 HYPH denotes /
T6068 20117-20118 CD denotes 2
T6066 20119-20124 NNS denotes cells
T6069 20125-20131 RB denotes stably
T6070 20132-20143 VBN denotes transfected
T6071 20144-20148 IN denotes with
T6072 20149-20155 CC denotes either
T6074 20156-20162 NN denotes pSuper
T6075 20162-20163 HYPH denotes -
T6073 20163-20168 NN denotes retro
T6076 20169-20170 -LRB- denotes (
T6077 20170-20177 NN denotes control
T6078 20177-20178 -RRB- denotes )
T6079 20179-20182 CC denotes and
T6080 20183-20189 NN denotes pSuper
T6082 20189-20190 HYPH denotes -
T6081 20190-20195 NN denotes retro
T6083 20196-20205 VBG denotes harboring
T6084 20206-20207 DT denotes a
T6086 20208-20213 JJ denotes short
T6085 20214-20221 NN denotes hairpin
T6087 20222-20229 IN denotes against
T6088 20230-20235 NN denotes Sam68
T6089 20236-20237 -LRB- denotes (
T6090 20237-20244 NN denotes Sam68sh
T6091 20244-20245 -RRB- denotes )
T6092 20246-20250 VBD denotes were
T6063 20251-20259 VBN denotes selected
T6093 20260-20264 IN denotes with
T6094 20265-20274 NN denotes puromycin
T6095 20274-20275 . denotes .
T6096 20275-20416 sentence denotes The expression of Sam68 was reduced by approximately 80% as evidenced by immunoblot analyses using β-actin as a loading control (Figure 8A).
T6097 20276-20279 DT denotes The
T6098 20280-20290 NN denotes expression
T6100 20291-20293 IN denotes of
T6101 20294-20299 NN denotes Sam68
T6102 20300-20303 VBD denotes was
T6099 20304-20311 VBN denotes reduced
T6103 20312-20314 IN denotes by
T6104 20315-20328 RB denotes approximately
T6105 20329-20331 CD denotes 80
T6106 20331-20332 NN denotes %
T6107 20333-20335 IN denotes as
T6108 20336-20345 VBN denotes evidenced
T6109 20346-20348 IN denotes by
T6110 20349-20359 NN denotes immunoblot
T6111 20360-20368 NNS denotes analyses
T6112 20369-20374 VBG denotes using
T6113 20375-20376 NN denotes β
T6115 20376-20377 HYPH denotes -
T6114 20377-20382 NN denotes actin
T6116 20383-20385 IN denotes as
T6117 20386-20387 DT denotes a
T6119 20388-20395 NN denotes loading
T6118 20396-20403 NN denotes control
T6120 20404-20405 -LRB- denotes (
T6122 20405-20411 NN denotes Figure
T6121 20412-20414 NN denotes 8A
T6123 20414-20415 -RRB- denotes )
T6124 20415-20416 . denotes .
T6125 20416-20561 sentence denotes Osteoblast differentiation was induced in cultures expressing pSuper-retro or pSuper-retro Sam68sh by addition of BMP-2 to the culture medium. .
T6126 20417-20427 NN denotes Osteoblast
T6127 20428-20443 NN denotes differentiation
T6129 20444-20447 VBD denotes was
T6128 20448-20455 VBN denotes induced
T6130 20456-20458 IN denotes in
T6131 20459-20467 NNS denotes cultures
T6132 20468-20478 VBG denotes expressing
T6133 20479-20485 NN denotes pSuper
T6135 20485-20486 HYPH denotes -
T6134 20486-20491 NN denotes retro
T6136 20492-20494 CC denotes or
T6137 20495-20501 NN denotes pSuper
T6139 20501-20502 HYPH denotes -
T6138 20502-20507 NN denotes retro
T6140 20508-20515 NN denotes Sam68sh
T6141 20516-20518 IN denotes by
T6142 20519-20527 NN denotes addition
T6143 20528-20530 IN denotes of
T6144 20531-20534 NN denotes BMP
T6145 20534-20535 HYPH denotes -
T6146 20535-20536 CD denotes 2
T6147 20537-20539 IN denotes to
T6148 20540-20543 DT denotes the
T6150 20544-20551 NN denotes culture
T6149 20552-20558 NN denotes medium
T6151 20558-20559 . denotes .
T6152 20560-20561 . denotes .
T6153 20561-20802 sentence denotes The expression of OCN and β-actin mRNAs was examined by semiquantitative RT-PCR and the Sam68sh-expressing cells displayed a more pronounced osteoblast phenotype compared with control cells, as assessed by the expression of OCN (Figure 8B).
T6154 20562-20565 DT denotes The
T6155 20566-20576 NN denotes expression
T6157 20577-20579 IN denotes of
T6158 20580-20583 NN denotes OCN
T6159 20584-20587 CC denotes and
T6160 20588-20589 NN denotes β
T6162 20589-20590 HYPH denotes -
T6161 20590-20595 NN denotes actin
T6163 20596-20601 NNS denotes mRNAs
T6164 20602-20605 VBD denotes was
T6156 20606-20614 VBN denotes examined
T6165 20615-20617 IN denotes by
T6166 20618-20634 JJ denotes semiquantitative
T6168 20635-20637 NN denotes RT
T6169 20637-20638 HYPH denotes -
T6167 20638-20641 NN denotes PCR
T6170 20642-20645 CC denotes and
T6171 20646-20649 DT denotes the
T6173 20650-20657 NN denotes Sam68sh
T6175 20657-20658 HYPH denotes -
T6174 20658-20668 VBG denotes expressing
T6172 20669-20674 NNS denotes cells
T6176 20675-20684 VBD denotes displayed
T6177 20685-20686 DT denotes a
T6179 20687-20691 RBR denotes more
T6180 20692-20702 JJ denotes pronounced
T6181 20703-20713 NN denotes osteoblast
T6178 20714-20723 NN denotes phenotype
T6182 20724-20732 VBN denotes compared
T6183 20733-20737 IN denotes with
T6184 20738-20745 NN denotes control
T6185 20746-20751 NNS denotes cells
T6186 20751-20753 , denotes ,
T6187 20753-20755 IN denotes as
T6188 20756-20764 VBN denotes assessed
T6189 20765-20767 IN denotes by
T6190 20768-20771 DT denotes the
T6191 20772-20782 NN denotes expression
T6192 20783-20785 IN denotes of
T6193 20786-20789 NN denotes OCN
T6194 20790-20791 -LRB- denotes (
T6196 20791-20797 NN denotes Figure
T6195 20798-20800 NN denotes 8B
T6197 20800-20801 -RRB- denotes )
T6198 20801-20802 . denotes .
T6199 20802-21935 sentence denotes Figure 8 Enhanced Osteogenic Differentiation of the C3HT101/2 Embryonic Cell Line Depleted of Endogenous Sam68 (A) C3HT101/2 cells transfected with an empty vector (pSuper-retro) or a vector containing an shRNA (Sam68 shRNA) were selected with puromycin, and knockdown populations depleted of Sam68 were identified. The reduction in Sam68 protein was analyzed by immunoblotting with anti-Sam68 (AD1) antibody and anti–β-actin antibodies as loading controls. (B) Osteogenic differentiation was carried out with conditioned medium containing BMP-2 for the indicated times. To assess the level of osteogenic differentiation in these cells, expression of late osteoblast marker, osteocalcin (OCN), was analyzed by RT-PCR and compared with β-actin and GAPDH controls. The DNA fragments were visualized by agarose gel stained with ethidium bromide. Given the apparent enhancement of mineralized nodule formation by Sam68−/− bone marrow stromal cells ex vivo and the phenotype observed with short hairpin RNA (shRNA)-treated C3H10T1/2, we stained sections of bone from 4- and 12-month-old mice for evidence of changes in marrow adiposity.
T6200 21647-21652 VBN denotes Given
T6202 21653-21656 DT denotes the
T6204 21657-21665 JJ denotes apparent
T6203 21666-21677 NN denotes enhancement
T6205 21678-21680 IN denotes of
T6206 21681-21692 VBN denotes mineralized
T6207 21693-21699 NN denotes nodule
T6208 21700-21709 NN denotes formation
T6209 21710-21712 IN denotes by
T6210 21713-21718 NN denotes Sam68
T6212 21718-21719 SYM denotes −
T6213 21719-21720 HYPH denotes /
T6214 21720-21721 SYM denotes −
T6215 21722-21726 NN denotes bone
T6216 21727-21733 NN denotes marrow
T6217 21734-21741 JJ denotes stromal
T6211 21742-21747 NNS denotes cells
T6218 21748-21750 FW denotes ex
T6219 21751-21755 FW denotes vivo
T6220 21756-21759 CC denotes and
T6221 21760-21763 DT denotes the
T6222 21764-21773 NN denotes phenotype
T6223 21774-21782 VBN denotes observed
T6224 21783-21787 IN denotes with
T6225 21788-21793 JJ denotes short
T6227 21794-21801 NN denotes hairpin
T6226 21802-21805 NN denotes RNA
T6229 21806-21807 -LRB- denotes (
T6230 21807-21812 NN denotes shRNA
T6231 21812-21813 -RRB- denotes )
T6232 21813-21814 HYPH denotes -
T6228 21814-21821 VBN denotes treated
T6233 21822-21829 NN denotes C3H10T1
T6234 21829-21830 HYPH denotes /
T6235 21830-21831 CD denotes 2
T6236 21831-21833 , denotes ,
T6237 21833-21835 PRP denotes we
T6201 21836-21843 VBD denotes stained
T6238 21844-21852 NNS denotes sections
T6239 21853-21855 IN denotes of
T6240 21856-21860 NN denotes bone
T6241 21861-21865 IN denotes from
T6242 21866-21867 CD denotes 4
T6244 21867-21868 HYPH denotes -
T6245 21869-21872 CC denotes and
T6246 21873-21875 CD denotes 12
T6247 21875-21876 HYPH denotes -
T6243 21876-21881 NN denotes month
T6249 21881-21882 HYPH denotes -
T6248 21882-21885 JJ denotes old
T6250 21886-21890 NNS denotes mice
T6251 21891-21894 IN denotes for
T6252 21895-21903 NN denotes evidence
T6253 21904-21906 IN denotes of
T6254 21907-21914 NNS denotes changes
T6255 21915-21917 IN denotes in
T6256 21918-21924 NN denotes marrow
T6257 21925-21934 NN denotes adiposity
T6258 21934-21935 . denotes .
T6259 21935-22253 sentence denotes Figure 9 shows sections of undecalcified bone from 4- and 12-month-old Sam68+/+ and Sam68−/− mice stained with von Kossa and toluidine blue to show mineralized tissue (Figure 9A, black) and marrow adipocytes (Figure 9B, white) and left unstained to show fluorochrome labeling of the mineralization fronts (Figure 9C).
T6260 21936-21942 NN denotes Figure
T6262 21943-21944 CD denotes 9
T6261 21945-21950 VBZ denotes shows
T6263 21951-21959 NNS denotes sections
T6264 21960-21962 IN denotes of
T6265 21963-21976 JJ denotes undecalcified
T6266 21977-21981 NN denotes bone
T6267 21982-21986 IN denotes from
T6268 21987-21988 CD denotes 4
T6270 21988-21989 HYPH denotes -
T6271 21990-21993 CC denotes and
T6272 21994-21996 CD denotes 12
T6273 21996-21997 HYPH denotes -
T6269 21997-22002 NN denotes month
T6275 22002-22003 HYPH denotes -
T6274 22003-22006 JJ denotes old
T6277 22007-22012 NN denotes Sam68
T6278 22012-22013 SYM denotes +
T6279 22013-22014 HYPH denotes /
T6280 22014-22015 SYM denotes +
T6281 22016-22019 CC denotes and
T6282 22020-22025 NN denotes Sam68
T6283 22025-22026 SYM denotes −
T6284 22026-22027 HYPH denotes /
T6285 22027-22028 SYM denotes −
T6276 22029-22033 NNS denotes mice
T6286 22034-22041 VBN denotes stained
T6287 22042-22046 IN denotes with
T6288 22047-22050 NNP denotes von
T6289 22051-22056 NNP denotes Kossa
T6290 22057-22060 CC denotes and
T6291 22061-22070 NN denotes toluidine
T6292 22071-22075 JJ denotes blue
T6293 22076-22078 TO denotes to
T6294 22079-22083 VB denotes show
T6295 22084-22095 VBN denotes mineralized
T6296 22096-22102 NN denotes tissue
T6297 22103-22104 -LRB- denotes (
T6299 22104-22110 NN denotes Figure
T6298 22111-22113 NN denotes 9A
T6300 22113-22115 , denotes ,
T6301 22115-22120 JJ denotes black
T6302 22120-22121 -RRB- denotes )
T6303 22122-22125 CC denotes and
T6304 22126-22132 NN denotes marrow
T6305 22133-22143 NNS denotes adipocytes
T6306 22144-22145 -LRB- denotes (
T6308 22145-22151 NN denotes Figure
T6307 22152-22154 NN denotes 9B
T6309 22154-22156 , denotes ,
T6310 22156-22161 JJ denotes white
T6311 22161-22162 -RRB- denotes )
T6312 22163-22166 CC denotes and
T6313 22167-22171 VBD denotes left
T6314 22172-22181 JJ denotes unstained
T6315 22182-22184 TO denotes to
T6316 22185-22189 VB denotes show
T6317 22190-22202 NN denotes fluorochrome
T6318 22203-22211 NN denotes labeling
T6319 22212-22214 IN denotes of
T6320 22215-22218 DT denotes the
T6322 22219-22233 NN denotes mineralization
T6321 22234-22240 NNS denotes fronts
T6323 22241-22242 -LRB- denotes (
T6325 22242-22248 NN denotes Figure
T6324 22249-22251 NN denotes 9C
T6326 22251-22252 -RRB- denotes )
T6327 22252-22253 . denotes .
T6328 22253-22506 sentence denotes Twelve-month-old Sam68+/+ mice showed a noticeable decrease in trabecular bone (Figure 9A), which was associated with a significant increase in marrow adiposity (Figure 9B) and with the two fluorochrome labels superimposed upon one another (Figure 9C).
T6329 22254-22260 CD denotes Twelve
T6331 22260-22261 HYPH denotes -
T6330 22261-22266 NN denotes month
T6333 22266-22267 HYPH denotes -
T6332 22267-22270 JJ denotes old
T6335 22271-22276 NN denotes Sam68
T6336 22276-22277 SYM denotes +
T6337 22277-22278 HYPH denotes /
T6338 22278-22279 SYM denotes +
T6334 22280-22284 NNS denotes mice
T6339 22285-22291 VBD denotes showed
T6340 22292-22293 DT denotes a
T6342 22294-22304 JJ denotes noticeable
T6341 22305-22313 NN denotes decrease
T6343 22314-22316 IN denotes in
T6344 22317-22327 JJ denotes trabecular
T6345 22328-22332 NN denotes bone
T6346 22333-22334 -LRB- denotes (
T6348 22334-22340 NN denotes Figure
T6347 22341-22343 NN denotes 9A
T6349 22343-22344 -RRB- denotes )
T6350 22344-22346 , denotes ,
T6351 22346-22351 WDT denotes which
T6353 22352-22355 VBD denotes was
T6352 22356-22366 VBN denotes associated
T6354 22367-22371 IN denotes with
T6355 22372-22373 DT denotes a
T6357 22374-22385 JJ denotes significant
T6356 22386-22394 NN denotes increase
T6358 22395-22397 IN denotes in
T6359 22398-22404 NN denotes marrow
T6360 22405-22414 NN denotes adiposity
T6361 22415-22416 -LRB- denotes (
T6363 22416-22422 NN denotes Figure
T6362 22423-22425 NN denotes 9B
T6364 22425-22426 -RRB- denotes )
T6365 22427-22430 CC denotes and
T6366 22431-22435 IN denotes with
T6367 22436-22439 DT denotes the
T6369 22440-22443 CD denotes two
T6370 22444-22456 NN denotes fluorochrome
T6368 22457-22463 NNS denotes labels
T6371 22464-22476 VBN denotes superimposed
T6372 22477-22481 IN denotes upon
T6373 22482-22485 CD denotes one
T6374 22486-22493 DT denotes another
T6375 22494-22495 -LRB- denotes (
T6377 22495-22501 NN denotes Figure
T6376 22502-22504 NN denotes 9C
T6378 22504-22505 -RRB- denotes )
T6379 22505-22506 . denotes .
T6380 22506-22629 sentence denotes In contrast, the bones of 12-month-old Sam68−/− mice appeared similar to those of the 4-month-old mice of either genotype.
T6381 22507-22509 IN denotes In
T6383 22510-22518 NN denotes contrast
T6384 22518-22520 , denotes ,
T6385 22520-22523 DT denotes the
T6386 22524-22529 NNS denotes bones
T6387 22530-22532 IN denotes of
T6388 22533-22535 CD denotes 12
T6390 22535-22536 HYPH denotes -
T6389 22536-22541 NN denotes month
T6392 22541-22542 HYPH denotes -
T6391 22542-22545 JJ denotes old
T6394 22546-22551 NN denotes Sam68
T6395 22551-22552 SYM denotes −
T6396 22552-22553 HYPH denotes /
T6397 22553-22554 SYM denotes −
T6393 22555-22559 NNS denotes mice
T6382 22560-22568 VBD denotes appeared
T6398 22569-22576 JJ denotes similar
T6399 22577-22579 IN denotes to
T6400 22580-22585 DT denotes those
T6401 22586-22588 IN denotes of
T6402 22589-22592 DT denotes the
T6404 22593-22594 CD denotes 4
T6406 22594-22595 HYPH denotes -
T6405 22595-22600 NN denotes month
T6408 22600-22601 HYPH denotes -
T6407 22601-22604 JJ denotes old
T6403 22605-22609 NNS denotes mice
T6409 22610-22612 IN denotes of
T6410 22613-22619 DT denotes either
T6411 22620-22628 NN denotes genotype
T6412 22628-22629 . denotes .
T6413 22629-22817 sentence denotes These data demonstrate that Sam68 regulates the differentiation of bone marrow mesenchymal cells to promote adipocyte differentiation and inhibit osteoblast differentiation in aging bone.
T6414 22630-22635 DT denotes These
T6415 22636-22640 NNS denotes data
T6416 22641-22652 VBP denotes demonstrate
T6417 22653-22657 IN denotes that
T6419 22658-22663 NN denotes Sam68
T6418 22664-22673 VBZ denotes regulates
T6420 22674-22677 DT denotes the
T6421 22678-22693 NN denotes differentiation
T6422 22694-22696 IN denotes of
T6423 22697-22701 NN denotes bone
T6424 22702-22708 NN denotes marrow
T6426 22709-22720 JJ denotes mesenchymal
T6425 22721-22726 NNS denotes cells
T6427 22727-22729 TO denotes to
T6428 22730-22737 VB denotes promote
T6429 22738-22747 NN denotes adipocyte
T6430 22748-22763 NN denotes differentiation
T6431 22764-22767 CC denotes and
T6432 22768-22775 VB denotes inhibit
T6433 22776-22786 NN denotes osteoblast
T6434 22787-22802 NN denotes differentiation
T6435 22803-22805 IN denotes in
T6436 22806-22811 VBG denotes aging
T6437 22812-22816 NN denotes bone
T6438 22816-22817 . denotes .
R1155 T2061 T2062 nsubjpass Sam68,Expressed
R1156 T2063 T2062 auxpass Is,Expressed
R1157 T2064 T2062 prep in,Expressed
R1158 T2065 T2066 det the,Skeleton
R1159 T2066 T2064 pobj Skeleton,in
R1160 T2067 T2066 amod Developing,Skeleton
R1161 T2068 T2066 prep of,Skeleton
R1162 T2069 T2070 amod Embryonic,Mice
R1163 T2070 T2068 pobj Mice,of
R1164 T2072 T2073 det The,mRNA
R1165 T2073 T2075 nsubjpass mRNA,known
R1166 T2074 T2073 compound Sam68,mRNA
R1167 T2076 T2075 auxpass is,known
R1168 T2077 T2078 aux to,expressed
R1169 T2078 T2075 xcomp expressed,known
R1170 T2079 T2078 auxpass be,expressed
R1171 T2080 T2078 advmod widely,expressed
R1172 T2081 T2082 punct [,38
R1173 T2082 T2078 parataxis 38,expressed
R1174 T2083 T2082 punct ],38
R1175 T2084 T2078 punct ", ",expressed
R1176 T2085 T2086 mark whereas,remains
R1177 T2086 T2078 advcl remains,expressed
R1178 T2087 T2088 poss its,pattern
R1179 T2088 T2086 nsubj pattern,remains
R1180 T2089 T2088 prep of,pattern
R1181 T2090 T2091 compound protein,expression
R1182 T2091 T2089 pobj expression,of
R1183 T2092 T2093 advmod in,vivo
R1184 T2093 T2088 advmod vivo,pattern
R1185 T2094 T2095 aux to,defined
R1186 T2095 T2086 xcomp defined,remains
R1187 T2096 T2095 auxpass be,defined
R1188 T2097 T2075 punct .,known
R1189 T2099 T2100 nsubjpass Sections,immunostained
R1190 T2101 T2099 prep of,Sections
R1191 T2102 T2103 npadvmod paraffin,embedded
R1192 T2103 T2105 amod embedded,mice
R1193 T2104 T2103 punct -,embedded
R1194 T2105 T2101 pobj mice,of
R1195 T2106 T2107 amod wild,type
R1196 T2107 T2105 nmod type,mice
R1197 T2108 T2107 punct -,type
R1198 T2109 T2105 nmod E14.5,mice
R1199 T2110 T2109 cc and,E14.5
R1200 T2111 T2109 conj E16.5,E14.5
R1201 T2112 T2105 amod embryonic,mice
R1202 T2113 T2100 auxpass were,immunostained
R1203 T2114 T2100 prep with,immunostained
R1204 T2115 T2116 det the,antibody
R1205 T2116 T2114 pobj antibody,with
R1206 T2117 T2118 advmod well,characterized
R1207 T2118 T2116 amod characterized,antibody
R1208 T2119 T2118 punct -,characterized
R1209 T2120 T2116 nmod AD1,antibody
R1210 T2121 T2116 amod anti-Sam68,antibody
R1211 T2122 T2116 punct ", ",antibody
R1212 T2123 T2116 acl raised,antibody
R1213 T2124 T2123 prep in,raised
R1214 T2125 T2124 pobj rabbits,in
R1215 T2126 T2116 prep against,antibody
R1216 T2127 T2128 det an,peptide
R1217 T2128 T2126 pobj peptide,against
R1218 T2129 T2128 amod immunizing,peptide
R1219 T2130 T2131 dep that,corresponds
R1220 T2131 T2128 relcl corresponds,peptide
R1221 T2132 T2131 prep to,corresponds
R1222 T2133 T2134 nmod amino,330
R1223 T2134 T2132 pobj 330,to
R1224 T2135 T2134 nmod acids,330
R1225 T2136 T2134 prep to,330
R1226 T2137 T2136 pobj 348,to
R1227 T2138 T2134 prep of,330
R1228 T2139 T2140 det the,protein
R1229 T2140 T2138 pobj protein,of
R1230 T2141 T2140 compound mouse,protein
R1231 T2142 T2140 compound Sam68,protein
R1232 T2143 T2144 punct [,46
R1233 T2144 T2100 parataxis 46,immunostained
R1234 T2145 T2144 punct ],46
R1235 T2146 T2100 punct .,immunostained
R1236 T2148 T2149 compound Sam68,immunoreactivity
R1237 T2149 T2150 nsubjpass immunoreactivity,observed
R1238 T2151 T2150 auxpass was,observed
R1239 T2152 T2150 prep in,observed
R1240 T2153 T2154 det the,skeleton
R1241 T2154 T2152 pobj skeleton,in
R1242 T2155 T2154 cc and,skeleton
R1243 T2156 T2157 amod soft,tissues
R1244 T2157 T2154 conj tissues,skeleton
R1245 T2158 T2154 prep of,skeleton
R1246 T2159 T2160 amod developing,mice
R1247 T2160 T2158 pobj mice,of
R1248 T2161 T2162 amod wild,type
R1249 T2162 T2160 compound type,mice
R1250 T2163 T2162 punct -,type
R1251 T2164 T2150 prep at,observed
R1252 T2165 T2164 pobj E14.5,at
R1253 T2166 T2165 cc and,E14.5
R1254 T2167 T2165 conj E16.5,E14.5
R1255 T2168 T2169 punct (,Figure
R1256 T2169 T2150 parataxis Figure,observed
R1257 T2170 T2169 nummod 1,Figure
R1258 T2171 T2169 punct ),Figure
R1259 T2172 T2150 punct .,observed
R1260 T2174 T2175 amod Intense,staining
R1261 T2175 T2176 nsubjpass staining,seen
R1262 T2177 T2176 auxpass was,seen
R1263 T2178 T2176 prep in,seen
R1264 T2179 T2180 det the,nucleus
R1265 T2180 T2178 pobj nucleus,in
R1266 T2181 T2180 prep of,nucleus
R1267 T2182 T2181 pobj cells,of
R1268 T2183 T2176 prep in,seen
R1269 T2184 T2185 det the,brain
R1270 T2185 T2183 pobj brain,in
R1271 T2186 T2185 amod developing,brain
R1272 T2187 T2185 punct ", ",brain
R1273 T2188 T2185 conj heart,brain
R1274 T2189 T2188 punct ", ",heart
R1275 T2190 T2188 cc and,heart
R1276 T2191 T2192 amod small,intestine
R1277 T2192 T2188 conj intestine,heart
R1278 T2193 T2194 punct (,1B
R1279 T2194 T2185 parataxis 1B,brain
R1280 T2195 T2194 compound Figure,1B
R1281 T2196 T2194 punct ),1B
R1282 T2197 T2183 punct ", ",in
R1283 T2198 T2199 advmod as,as
R1284 T2199 T2183 cc as,in
R1285 T2200 T2199 advmod well,as
R1286 T2201 T2183 conj in,in
R1287 T2202 T2201 pobj chondrocytes,in
R1288 T2203 T2202 prep in,chondrocytes
R1289 T2204 T2205 det the,septum
R1290 T2205 T2203 pobj septum,in
R1291 T2206 T2205 amod nasal,septum
R1292 T2207 T2205 cc and,septum
R1293 T2208 T2209 det the,tissue
R1294 T2209 T2205 conj tissue,septum
R1295 T2210 T2209 amod glandular,tissue
R1296 T2211 T2209 amod adjacent,tissue
R1297 T2212 T2211 prep to,adjacent
R1298 T2213 T2214 det the,cartilage
R1299 T2214 T2212 pobj cartilage,to
R1300 T2215 T2214 amod nasal,cartilage
R1301 T2216 T2217 punct (,1C
R1302 T2217 T2202 parataxis 1C,chondrocytes
R1303 T2218 T2217 compound Figure,1C
R1304 T2219 T2217 punct ", ",1C
R1305 T2220 T2221 compound panels,A
R1306 T2221 T2217 appos A,1C
R1307 T2222 T2223 punct –,D
R1308 T2223 T2221 prep D,A
R1309 T2224 T2217 punct ),1C
R1310 T2225 T2201 punct ", ",in
R1311 T2226 T2201 prep in,in
R1312 T2227 T2228 det the,vertebra
R1313 T2228 T2226 pobj vertebra,in
R1314 T2229 T2228 cc and,vertebra
R1315 T2230 T2231 amod intervertebral,discs
R1316 T2231 T2228 conj discs,vertebra
R1317 T2232 T2233 punct (,E
R1318 T2233 T2228 parataxis E,vertebra
R1319 T2234 T2233 compound panels,E
R1320 T2235 T2236 punct –,H
R1321 T2236 T2233 prep H,E
R1322 T2237 T2233 punct ),E
R1323 T2238 T2226 cc and,in
R1324 T2239 T2226 conj in,in
R1325 T2240 T2241 det the,epiphysis
R1326 T2241 T2239 pobj epiphysis,in
R1327 T2242 T2243 punct (,I
R1328 T2243 T2241 parataxis I,epiphysis
R1329 T2244 T2243 compound panels,I
R1330 T2245 T2246 punct –,K
R1331 T2246 T2243 prep K,I
R1332 T2247 T2243 punct ),I
R1333 T2248 T2241 cc and,epiphysis
R1334 T2249 T2241 conj metaphysis,epiphysis
R1335 T2250 T2251 punct (,L
R1336 T2251 T2249 parataxis L,metaphysis
R1337 T2252 T2251 compound panel,L
R1338 T2253 T2251 punct ),L
R1339 T2254 T2241 prep of,epiphysis
R1340 T2255 T2256 amod long,bones
R1341 T2256 T2254 pobj bones,of
R1342 T2257 T2176 punct .,seen
R1343 T2259 T2260 amod Nuclear,staining
R1344 T2260 T2261 nsubjpass staining,observed
R1345 T2262 T2261 auxpass was,observed
R1346 T2263 T2261 prep in,observed
R1347 T2264 T2265 amod proliferating,chondrocytes
R1348 T2265 T2263 pobj chondrocytes,in
R1349 T2266 T2265 cc and,chondrocytes
R1350 T2267 T2268 amod hypertrophic,chondrocytes
R1351 T2268 T2265 conj chondrocytes,chondrocytes
R1352 T2269 T2270 punct (,1C
R1353 T2270 T2268 parataxis 1C,chondrocytes
R1354 T2271 T2270 compound Figure,1C
R1355 T2272 T2270 punct ", ",1C
R1356 T2273 T2274 compound panel,H
R1357 T2274 T2270 appos H,1C
R1358 T2275 T2270 punct ),1C
R1359 T2276 T2263 punct ", ",in
R1360 T2277 T2263 conj in,in
R1361 T2278 T2277 pobj osteoblasts,in
R1362 T2279 T2280 punct (,L
R1363 T2280 T2278 parataxis L,osteoblasts
R1364 T2281 T2280 compound panel,L
R1365 T2282 T2280 punct ;,L
R1366 T2283 T2284 compound Dataset,S1
R1367 T2284 T2280 appos S1,L
R1368 T2285 T2280 punct ),L
R1369 T2286 T2277 punct ", ",in
R1370 T2287 T2277 cc and,in
R1371 T2288 T2277 conj in,in
R1372 T2289 T2288 pobj osteoclasts,in
R1373 T2290 T2291 punct (,S1
R1374 T2291 T2289 parataxis S1,osteoclasts
R1375 T2292 T2291 compound Dataset,S1
R1376 T2293 T2291 punct ),S1
R1377 T2294 T2261 punct .,observed
R1378 T2296 T2297 det No,staining
R1379 T2297 T2298 nsubjpass staining,observed
R1380 T2299 T2298 auxpass was,observed
R1381 T2300 T2298 prep with,observed
R1382 T2301 T2302 amod preimmune,serum
R1383 T2302 T2300 pobj serum,with
R1384 T2303 T2300 cc or,with
R1385 T2304 T2305 advmod when,preadsorbed
R1386 T2305 T2300 conj preadsorbed,with
R1387 T2306 T2307 det the,antiserum
R1388 T2307 T2305 nsubjpass antiserum,preadsorbed
R1389 T2308 T2305 auxpass was,preadsorbed
R1390 T2309 T2305 prep with,preadsorbed
R1391 T2310 T2311 det the,peptide
R1392 T2311 T2309 pobj peptide,with
R1393 T2312 T2311 amod immunizing,peptide
R1394 T2313 T2314 punct (,1C
R1395 T2314 T2305 parataxis 1C,preadsorbed
R1396 T2315 T2314 compound Figure,1C
R1397 T2316 T2314 punct ", ",1C
R1398 T2317 T2318 compound panels,B
R1399 T2318 T2314 appos B,1C
R1400 T2319 T2318 punct ", ",B
R1401 T2320 T2318 conj F,B
R1402 T2321 T2320 punct ", ",F
R1403 T2322 T2320 cc and,F
R1404 T2323 T2320 conj J,F
R1405 T2324 T2314 punct ),1C
R1406 T2325 T2298 punct .,observed
R1407 T2327 T2328 advcl Taken,demonstrate
R1408 T2329 T2327 advmod together,Taken
R1409 T2330 T2328 punct ", ",demonstrate
R1410 T2331 T2332 det these,data
R1411 T2332 T2328 nsubj data,demonstrate
R1412 T2333 T2334 mark that,expressed
R1413 T2334 T2328 ccomp expressed,demonstrate
R1414 T2335 T2336 compound Sam68,protein
R1415 T2336 T2334 nsubjpass protein,expressed
R1416 T2337 T2334 auxpass is,expressed
R1417 T2338 T2334 advmod selectively,expressed
R1418 T2339 T2334 prep in,expressed
R1419 T2340 T2341 det the,embryo
R1420 T2341 T2339 pobj embryo,in
R1421 T2342 T2341 amod developing,embryo
R1422 T2343 T2341 compound mouse,embryo
R1423 T2344 T2334 punct ", ",expressed
R1424 T2345 T2334 prep with,expressed
R1425 T2346 T2347 advmod particularly,elevated
R1426 T2347 T2348 amod elevated,expression
R1427 T2348 T2345 pobj expression,with
R1428 T2349 T2348 prep in,expression
R1429 T2350 T2349 pobj cartilage,in
R1430 T2351 T2350 cc and,cartilage
R1431 T2352 T2350 conj bone,cartilage
R1432 T2353 T2328 punct .,demonstrate
R1433 T2520 T2521 nsubj Sam68,is
R1434 T2522 T2521 neg Not,is
R1435 T2523 T2521 acomp Essential,is
R1436 T2524 T2523 prep for,Essential
R1437 T2525 T2526 compound Mouse,Development
R1438 T2526 T2524 pobj Development,for
R1439 T2528 T2529 aux To,define
R1440 T2529 T2530 advcl define,generated
R1441 T2531 T2532 det the,role
R1442 T2532 T2529 dobj role,define
R1443 T2533 T2532 amod physiologic,role
R1444 T2534 T2532 prep of,role
R1445 T2535 T2534 pobj Sam68,of
R1446 T2536 T2530 punct ", ",generated
R1447 T2537 T2530 nsubj we,generated
R1448 T2538 T2539 npadvmod Sam68,deficient
R1449 T2539 T2541 amod deficient,mice
R1450 T2540 T2539 punct -,deficient
R1451 T2541 T2530 dobj mice,generated
R1452 T2542 T2530 prep by,generated
R1453 T2543 T2544 amod targeted,disruption
R1454 T2544 T2542 pobj disruption,by
R1455 T2545 T2544 prep of,disruption
R1456 T2546 T2547 nmod exons,4
R1457 T2547 T2545 pobj 4,of
R1458 T2548 T2547 cc and,4
R1459 T2549 T2547 conj 5,4
R1460 T2550 T2547 prep of,4
R1461 T2551 T2552 det the,gene
R1462 T2552 T2550 pobj gene,of
R1463 T2553 T2552 compound sam68,gene
R1464 T2554 T2547 punct ", ",4
R1465 T2555 T2556 dep which,encode
R1466 T2556 T2547 relcl encode,4
R1467 T2557 T2558 det the,region
R1468 T2558 T2556 dobj region,encode
R1469 T2559 T2558 amod functional,region
R1470 T2560 T2558 prep of,region
R1471 T2561 T2562 det the,domain
R1472 T2562 T2560 pobj domain,of
R1473 T2563 T2564 compound KH,type
R1474 T2564 T2562 compound type,domain
R1475 T2565 T2564 punct -,type
R1476 T2566 T2567 compound RNA,binding
R1477 T2567 T2562 compound binding,domain
R1478 T2568 T2569 punct (,2A
R1479 T2569 T2530 parataxis 2A,generated
R1480 T2570 T2569 compound Figure,2A
R1481 T2571 T2569 punct ),2A
R1482 T2572 T2530 punct .,generated
R1483 T2574 T2575 det The,integrity
R1484 T2575 T2576 nsubjpass integrity,verified
R1485 T2577 T2575 prep of,integrity
R1486 T2578 T2579 det the,allele
R1487 T2579 T2577 pobj allele,of
R1488 T2580 T2579 amod targeted,allele
R1489 T2581 T2576 auxpass was,verified
R1490 T2582 T2576 prep by,verified
R1491 T2583 T2584 compound Southern,blot
R1492 T2584 T2585 compound blot,analysis
R1493 T2585 T2582 pobj analysis,by
R1494 T2586 T2587 punct (,2B
R1495 T2587 T2585 parataxis 2B,analysis
R1496 T2588 T2587 compound Figure,2B
R1497 T2589 T2587 punct ),2B
R1498 T2590 T2582 cc and,by
R1499 T2591 T2582 conj by,by
R1500 T2592 T2591 pobj PCR,by
R1501 T2593 T2592 prep of,PCR
R1502 T2594 T2595 amod genomic,DNA
R1503 T2595 T2593 pobj DNA,of
R1504 T2596 T2597 punct (,data
R1505 T2597 T2592 meta data,PCR
R1506 T2598 T2597 amod unpublished,data
R1507 T2599 T2597 punct ),data
R1508 T2600 T2576 punct .,verified
R1509 T2602 T2603 compound Sam68,transcripts
R1510 T2603 T2604 nsubj transcripts,were
R1511 T2605 T2603 acl encoded,transcripts
R1512 T2606 T2605 agent by,encoded
R1513 T2607 T2608 nmod exons,1
R1514 T2608 T2606 pobj 1,by
R1515 T2609 T2608 prep to,1
R1516 T2610 T2609 pobj 5,to
R1517 T2611 T2604 acomp absent,were
R1518 T2612 T2613 mark as,evidenced
R1519 T2613 T2604 advcl evidenced,were
R1520 T2614 T2613 prep by,evidenced
R1521 T2615 T2616 compound RT,PCR
R1522 T2616 T2614 pobj PCR,by
R1523 T2617 T2616 punct -,PCR
R1524 T2618 T2619 punct (,S2
R1525 T2619 T2613 parataxis S2,evidenced
R1526 T2620 T2619 compound Dataset,S2
R1527 T2621 T2619 punct ),S2
R1528 T2622 T2604 punct ", ",were
R1529 T2623 T2604 cc and,were
R1530 T2624 T2625 nmod Sam68,mice
R1531 T2625 T2629 nsubj mice,were
R1532 T2626 T2624 punct −,Sam68
R1533 T2627 T2624 punct /,Sam68
R1534 T2628 T2624 punct −,Sam68
R1535 T2629 T2604 conj were,were
R1536 T2630 T2629 acomp devoid,were
R1537 T2631 T2630 prep of,devoid
R1538 T2632 T2633 compound Sam68,protein
R1539 T2633 T2631 pobj protein,of
R1540 T2634 T2629 punct ", ",were
R1541 T2635 T2636 mark as,visualized
R1542 T2636 T2629 advcl visualized,were
R1543 T2637 T2636 prep by,visualized
R1544 T2638 T2637 pcomp immunoblotting,by
R1545 T2639 T2638 prep with,immunoblotting
R1546 T2640 T2641 amod anti-Sam68,AD1
R1547 T2641 T2642 nmod AD1,antibodies
R1548 T2642 T2639 pobj antibodies,with
R1549 T2643 T2641 cc and,AD1
R1550 T2644 T2641 conj SC,AD1
R1551 T2645 T2644 punct -,SC
R1552 T2646 T2644 nummod 333,SC
R1553 T2647 T2642 cc or,antibodies
R1554 T2648 T2649 nmod control,serum
R1555 T2649 T2642 conj serum,antibodies
R1556 T2650 T2649 amod normal,serum
R1557 T2651 T2649 compound rabbit,serum
R1558 T2652 T2649 cc or,serum
R1559 T2653 T2654 amod anti-Sam68,AD1
R1560 T2654 T2649 conj AD1,serum
R1561 T2655 T2654 acl preabsorbed,AD1
R1562 T2656 T2655 prep with,preabsorbed
R1563 T2657 T2656 pobj peptide,with
R1564 T2658 T2659 punct (,2C
R1565 T2659 T2629 parataxis 2C,were
R1566 T2660 T2659 compound Figure,2C
R1567 T2661 T2659 punct ),2C
R1568 T2662 T2629 punct .,were
R1569 T2664 T2665 nsubj SC,is
R1570 T2666 T2664 punct -,SC
R1571 T2667 T2664 nummod 333,SC
R1572 T2668 T2669 det a,antibody
R1573 T2669 T2665 attr antibody,is
R1574 T2670 T2669 nmod rabbit,antibody
R1575 T2671 T2669 amod anti-Sam68,antibody
R1576 T2672 T2673 dep that,raised
R1577 T2673 T2669 relcl raised,antibody
R1578 T2674 T2673 auxpass was,raised
R1579 T2675 T2673 prep against,raised
R1580 T2676 T2677 det the,acids
R1581 T2677 T2675 pobj acids,against
R1582 T2678 T2679 npadvmod C,terminal
R1583 T2679 T2677 amod terminal,acids
R1584 T2680 T2679 punct -,terminal
R1585 T2681 T2677 nummod 20,acids
R1586 T2682 T2677 compound amino,acids
R1587 T2683 T2677 prep of,acids
R1588 T2684 T2683 pobj Sam68,of
R1589 T2685 T2686 punct [,47
R1590 T2686 T2665 parataxis 47,is
R1591 T2687 T2686 punct ],47
R1592 T2688 T2665 punct .,is
R1593 T2690 T2691 det These,data
R1594 T2691 T2692 nsubj data,confirmed
R1595 T2693 T2694 det the,generation
R1596 T2694 T2692 dobj generation,confirmed
R1597 T2695 T2694 prep of,generation
R1598 T2696 T2697 det a,mouse
R1599 T2697 T2695 pobj mouse,of
R1600 T2698 T2697 amod deficient,mouse
R1601 T2699 T2698 prep in,deficient
R1602 T2700 T2699 pobj Sam68,in
R1603 T2701 T2692 punct .,confirmed
R1604 T2703 T2704 det The,genotypes
R1605 T2704 T2705 nsubj genotypes,exhibited
R1606 T2706 T2704 prep of,genotypes
R1607 T2707 T2706 pobj offspring,of
R1608 T2708 T2707 prep from,offspring
R1609 T2709 T2710 compound heterozygote,intercrosses
R1610 T2710 T2708 pobj intercrosses,from
R1611 T2711 T2712 det a,segregation
R1612 T2712 T2705 dobj segregation,exhibited
R1613 T2713 T2712 amod Mendelian,segregation
R1614 T2714 T2705 prep at,exhibited
R1615 T2715 T2714 pobj E18.5,at
R1616 T2716 T2717 punct (,Table
R1617 T2717 T2705 parataxis Table,exhibited
R1618 T2718 T2717 nummod 1,Table
R1619 T2719 T2717 punct ),Table
R1620 T2720 T2705 punct .,exhibited
R1621 T2722 T2723 prep Despite,died
R1622 T2724 T2725 det the,lack
R1623 T2725 T2722 pobj lack,Despite
R1624 T2726 T2725 prep of,lack
R1625 T2727 T2728 amod visible,deformity
R1626 T2728 T2726 pobj deformity,of
R1627 T2729 T2723 punct ", ",died
R1628 T2730 T2723 nsubj many,died
R1629 T2731 T2730 prep of,many
R1630 T2732 T2733 det the,pups
R1631 T2733 T2731 pobj pups,of
R1632 T2734 T2733 nmod Sam68,pups
R1633 T2735 T2734 punct −,Sam68
R1634 T2736 T2734 punct /,Sam68
R1635 T2737 T2734 punct −,Sam68
R1636 T2738 T2723 prep at,died
R1637 T2739 T2738 pobj birth,at
R1638 T2740 T2723 prep of,died
R1639 T2741 T2742 amod unknown,causes
R1640 T2742 T2740 pobj causes,of
R1641 T2743 T2723 punct .,died
R1642 T2745 T2746 nmod Sam68,mice
R1643 T2746 T2750 nsubj mice,were
R1644 T2747 T2745 punct +,Sam68
R1645 T2748 T2745 punct /,Sam68
R1646 T2749 T2745 punct -,Sam68
R1647 T2751 T2752 advmod phenotypically,normal
R1648 T2752 T2750 acomp normal,were
R1649 T2753 T2750 cc and,were
R1650 T2754 T2755 nmod Sam68,pups
R1651 T2755 T2759 nsubj pups,lived
R1652 T2756 T2754 punct −,Sam68
R1653 T2757 T2754 punct /,Sam68
R1654 T2758 T2754 punct −,Sam68
R1655 T2759 T2750 conj lived,were
R1656 T2760 T2761 dep that,survived
R1657 T2761 T2755 relcl survived,pups
R1658 T2762 T2763 det the,period
R1659 T2763 T2761 dobj period,survived
R1660 T2764 T2763 amod perinatal,period
R1661 T2765 T2759 advmod invariably,lived
R1662 T2766 T2759 prep to,lived
R1663 T2767 T2768 amod old,age
R1664 T2768 T2766 pobj age,to
R1665 T2769 T2759 punct .,lived
R1666 T2771 T2772 prep Despite,develop
R1667 T2773 T2771 pobj evidence,Despite
R1668 T2774 T2775 mark that,expressed
R1669 T2775 T2773 acl expressed,evidence
R1670 T2776 T2777 compound Sam68,mRNA
R1671 T2777 T2775 nsubjpass mRNA,expressed
R1672 T2778 T2775 auxpass is,expressed
R1673 T2779 T2775 advmod widely,expressed
R1674 T2780 T2775 cc and,expressed
R1675 T2781 T2775 conj phosphorylated,expressed
R1676 T2782 T2781 prep in,phosphorylated
R1677 T2783 T2782 pobj mitosis,in
R1678 T2784 T2785 punct [,42
R1679 T2785 T2775 parataxis 42,expressed
R1680 T2786 T2785 nummod 41,42
R1681 T2787 T2785 punct ",",42
R1682 T2788 T2785 punct ],42
R1683 T2789 T2772 punct ", ",develop
R1684 T2790 T2791 det the,mice
R1685 T2791 T2772 nsubj mice,develop
R1686 T2792 T2791 nmod Sam68,mice
R1687 T2793 T2792 punct −,Sam68
R1688 T2794 T2792 punct /,Sam68
R1689 T2795 T2792 punct −,Sam68
R1690 T2796 T2772 aux did,develop
R1691 T2797 T2772 neg not,develop
R1692 T2798 T2772 dobj tumors,develop
R1693 T2799 T2772 cc and,develop
R1694 T2800 T2772 conj showed,develop
R1695 T2801 T2802 det no,illnesses
R1696 T2802 T2800 dobj illnesses,showed
R1697 T2803 T2802 amod immunologic,illnesses
R1698 T2804 T2803 cc or,immunologic
R1699 T2805 T2806 amod other,major
R1700 T2806 T2803 conj major,immunologic
R1701 T2807 T2772 punct .,develop
R1702 T2809 T2810 nmod Sam68,mice
R1703 T2810 T2814 nsubj mice,have
R1704 T2811 T2809 punct −,Sam68
R1705 T2812 T2809 punct /,Sam68
R1706 T2813 T2809 punct −,Sam68
R1707 T2815 T2814 aux did,have
R1708 T2816 T2814 punct ", ",have
R1709 T2817 T2814 advmod however,have
R1710 T2818 T2814 punct ", ",have
R1711 T2819 T2814 dobj difficulty,have
R1712 T2820 T2819 acl breeding,difficulty
R1713 T2821 T2814 prep due,have
R1714 T2822 T2821 pcomp to,due
R1715 T2823 T2824 amod male,infertility
R1716 T2824 T2821 pobj infertility,due
R1717 T2825 T2814 cc and,have
R1718 T2826 T2827 det the,females
R1719 T2827 T2828 nsubj females,provided
R1720 T2828 T2814 conj provided,have
R1721 T2829 T2828 advmod rarely,provided
R1722 T2830 T2831 amod adequate,care
R1723 T2831 T2828 dobj care,provided
R1724 T2832 T2828 dative to,provided
R1725 T2833 T2834 poss their,young
R1726 T2834 T2832 pobj young,to
R1727 T2835 T2828 punct .,provided
R1732 T3035 T3036 compound Sam68,Deficiency
R1733 T3036 T3037 nsubj Deficiency,Protects
R1734 T3038 T3037 dobj Mice,Protects
R1735 T3039 T3037 prep from,Protects
R1736 T3040 T3041 npadvmod Age,Related
R1737 T3041 T3043 amod Related,Loss
R1738 T3042 T3041 punct -,Related
R1739 T3043 T3039 pobj Loss,from
R1740 T3044 T3043 compound Bone,Loss
R1741 T3046 T3047 nmod Src,animals
R1742 T3047 T3049 nsubjpass animals,known
R1743 T3048 T3047 amod null,animals
R1744 T3050 T3049 auxpass are,known
R1745 T3051 T3052 aux to,have
R1746 T3052 T3049 xcomp have,known
R1747 T3053 T3054 compound bone,metabolism
R1748 T3054 T3055 compound metabolism,defects
R1749 T3055 T3052 dobj defects,have
R1750 T3056 T3057 punct [,48
R1751 T3057 T3049 parataxis 48,known
R1752 T3058 T3057 punct ],48
R1753 T3059 T3049 punct ", ",known
R1754 T3060 T3049 cc and,known
R1755 T3061 T3062 mark since,is
R1756 T3062 T3064 advcl is,decided
R1757 T3063 T3062 nsubj Sam68,is
R1758 T3064 T3049 conj decided,known
R1759 T3065 T3066 det a,substrate
R1760 T3066 T3062 attr substrate,is
R1761 T3067 T3066 prep of,substrate
R1762 T3068 T3067 pobj Src,of
R1763 T3069 T3064 punct ", ",decided
R1764 T3070 T3064 nsubj we,decided
R1765 T3071 T3072 aux to,analyze
R1766 T3072 T3064 xcomp analyze,decided
R1767 T3073 T3074 det the,mice
R1768 T3074 T3072 dobj mice,analyze
R1769 T3075 T3074 compound Sam68,mice
R1770 T3076 T3072 prep for,analyze
R1771 T3077 T3078 amod skeletal,abnormalities
R1772 T3078 T3076 pobj abnormalities,for
R1773 T3079 T3064 punct .,decided
R1774 T3081 T3082 nsubjpass Cohorts,euthanized
R1775 T3083 T3081 prep of,Cohorts
R1776 T3084 T3085 nmod Sam68,mice
R1777 T3085 T3083 pobj mice,of
R1778 T3086 T3084 punct +,Sam68
R1779 T3087 T3084 punct /,Sam68
R1780 T3088 T3084 punct +,Sam68
R1781 T3089 T3084 cc and,Sam68
R1782 T3090 T3084 conj Sam68,Sam68
R1783 T3091 T3090 punct −,Sam68
R1784 T3092 T3090 punct /,Sam68
R1785 T3093 T3090 punct −,Sam68
R1786 T3094 T3082 auxpass were,euthanized
R1787 T3095 T3082 prep at,euthanized
R1788 T3096 T3097 nummod 4,months
R1789 T3097 T3095 pobj months,at
R1790 T3098 T3096 cc and,4
R1791 T3099 T3096 conj 12,4
R1792 T3100 T3097 prep of,months
R1793 T3101 T3100 pobj age,of
R1794 T3102 T3082 prep for,euthanized
R1795 T3103 T3104 amod skeletal,phenotyping
R1796 T3104 T3102 pobj phenotyping,for
R1797 T3105 T3082 punct .,euthanized
R1798 T3107 T3108 aux To,minimize
R1799 T3108 T3109 advcl minimize,selected
R1800 T3110 T3108 dobj differences,minimize
R1801 T3111 T3110 prep in,differences
R1802 T3112 T3113 det the,phenotype
R1803 T3113 T3111 pobj phenotype,in
R1804 T3114 T3113 compound bone,phenotype
R1805 T3115 T3116 dep that,arise
R1806 T3116 T3110 relcl arise,differences
R1807 T3117 T3116 aux might,arise
R1808 T3118 T3116 advcl secondary,arise
R1809 T3119 T3118 prep to,secondary
R1810 T3120 T3121 nmod gender,differences
R1811 T3121 T3119 pobj differences,to
R1812 T3122 T3120 cc or,gender
R1813 T3123 T3120 conj weight,gender
R1814 T3124 T3109 punct ", ",selected
R1815 T3125 T3109 nsubj we,selected
R1816 T3126 T3127 npadvmod age,matched
R1817 T3127 T3129 amod matched,mice
R1818 T3128 T3127 punct -,matched
R1819 T3129 T3109 dobj mice,selected
R1820 T3130 T3129 amod female,mice
R1821 T3131 T3109 prep for,selected
R1822 T3132 T3133 det these,analyses
R1823 T3133 T3131 pobj analyses,for
R1824 T3134 T3109 punct .,selected
R1825 T3136 T3137 det The,mice
R1826 T3137 T3139 nsubj mice,demonstrated
R1827 T3138 T3137 amod female,mice
R1828 T3140 T3141 amod similar,increases
R1829 T3141 T3139 dobj increases,demonstrated
R1830 T3142 T3141 prep in,increases
R1831 T3143 T3144 compound body,weight
R1832 T3144 T3142 pobj weight,in
R1833 T3145 T3146 punct ", ",weighed
R1834 T3146 T3141 parataxis weighed,increases
R1835 T3147 T3146 mark although,weighed
R1836 T3148 T3149 nummod 4,month
R1837 T3149 T3151 npadvmod month,old
R1838 T3150 T3149 punct -,month
R1839 T3151 T3153 amod old,mice
R1840 T3152 T3151 punct -,old
R1841 T3153 T3146 nsubj mice,weighed
R1842 T3154 T3153 nmod Sam68,mice
R1843 T3155 T3154 punct −,Sam68
R1844 T3156 T3154 punct /,Sam68
R1845 T3157 T3154 punct −,Sam68
R1846 T3158 T3159 amod less,than
R1847 T3159 T3146 prep than,weighed
R1848 T3160 T3161 nmod Sam68,mice
R1849 T3161 T3159 pobj mice,than
R1850 T3162 T3160 punct +,Sam68
R1851 T3163 T3160 punct /,Sam68
R1852 T3164 T3160 punct +,Sam68
R1853 T3165 T3141 punct ", ",increases
R1854 T3166 T3141 cc and,increases
R1855 T3167 T3168 amod similar,changes
R1856 T3168 T3141 conj changes,increases
R1857 T3169 T3168 prep in,changes
R1858 T3170 T3171 compound bone,lengths
R1859 T3171 T3169 pobj lengths,in
R1860 T3172 T3168 prep in,changes
R1861 T3173 T3174 det the,skeleton
R1862 T3174 T3172 pobj skeleton,in
R1863 T3175 T3174 amod axial,skeleton
R1864 T3176 T3175 cc and,axial
R1865 T3177 T3175 conj appendicular,axial
R1866 T3178 T3168 prep between,changes
R1867 T3179 T3180 nummod 4,months
R1868 T3180 T3178 pobj months,between
R1869 T3181 T3179 cc and,4
R1870 T3182 T3179 conj 12,4
R1871 T3183 T3180 prep of,months
R1872 T3184 T3183 pobj age,of
R1873 T3185 T3186 punct (,Table
R1874 T3186 T3139 parataxis Table,demonstrated
R1875 T3187 T3186 nummod 2,Table
R1876 T3188 T3186 punct ),Table
R1877 T3189 T3139 punct .,demonstrated
R1878 T3191 T3192 compound Faxitron,radiography
R1879 T3192 T3193 nsubj radiography,revealed
R1880 T3194 T3195 punct (,Corporation
R1881 T3195 T3192 parataxis Corporation,radiography
R1882 T3196 T3195 compound Faxitron,Corporation
R1883 T3197 T3198 compound X,ray
R1884 T3198 T3195 compound ray,Corporation
R1885 T3199 T3198 punct -,ray
R1886 T3200 T3195 punct ", ",Corporation
R1887 T3201 T3195 npadvmod Wheeling,Corporation
R1888 T3202 T3195 punct ", ",Corporation
R1889 T3203 T3195 npadvmod Illinois,Corporation
R1890 T3204 T3195 punct ", ",Corporation
R1891 T3205 T3206 compound United,States
R1892 T3206 T3195 npadvmod States,Corporation
R1893 T3207 T3195 punct ),Corporation
R1894 T3208 T3209 punct (,3A
R1895 T3209 T3192 parataxis 3A,radiography
R1896 T3210 T3209 compound Figure,3A
R1897 T3211 T3209 punct ),3A
R1898 T3212 T3192 cc and,radiography
R1899 T3213 T3214 amod micro–computed,tomography
R1900 T3214 T3192 conj tomography,radiography
R1901 T3215 T3216 punct (,CT
R1902 T3216 T3214 parataxis CT,tomography
R1903 T3217 T3216 punct ),CT
R1904 T3218 T3219 punct (,3B
R1905 T3219 T3214 parataxis 3B,tomography
R1906 T3220 T3219 compound Figure,3B
R1907 T3221 T3219 punct ),3B
R1908 T3222 T3223 amod significant,thinning
R1909 T3223 T3193 dobj thinning,revealed
R1910 T3224 T3223 amod cortical,thinning
R1911 T3225 T3226 punct (,arrow
R1912 T3226 T3223 parataxis arrow,thinning
R1913 T3227 T3226 punct ),arrow
R1914 T3228 T3223 cc and,thinning
R1915 T3229 T3230 det a,reduction
R1916 T3230 T3223 conj reduction,thinning
R1917 T3231 T3230 prep in,reduction
R1918 T3232 T3233 amod metaphyseal,bone
R1919 T3233 T3231 pobj bone,in
R1920 T3234 T3235 punct (,asterisk
R1921 T3235 T3230 parataxis asterisk,reduction
R1922 T3236 T3235 punct ),asterisk
R1923 T3237 T3223 prep in,thinning
R1924 T3238 T3239 det the,femora
R1925 T3239 T3237 pobj femora,in
R1926 T3240 T3239 amod distal,femora
R1927 T3241 T3239 prep of,femora
R1928 T3242 T3243 nummod 12,month
R1929 T3243 T3245 npadvmod month,old
R1930 T3244 T3243 punct -,month
R1931 T3245 T3247 amod old,mice
R1932 T3246 T3245 punct -,old
R1933 T3247 T3241 pobj mice,of
R1934 T3248 T3247 nmod Sam68,mice
R1935 T3249 T3248 punct +,Sam68
R1936 T3250 T3248 punct /,Sam68
R1937 T3251 T3248 punct +,Sam68
R1938 T3252 T3223 prep compared,thinning
R1939 T3253 T3252 prep with,compared
R1940 T3254 T3255 nummod 4,month
R1941 T3255 T3257 npadvmod month,old
R1942 T3256 T3255 punct -,month
R1943 T3257 T3259 amod old,mice
R1944 T3258 T3257 punct -,old
R1945 T3259 T3253 pobj mice,with
R1946 T3260 T3259 prep of,mice
R1947 T3261 T3262 preconj either,genotype
R1948 T3262 T3260 pobj genotype,of
R1949 T3263 T3259 cc and,mice
R1950 T3264 T3265 nummod 12,month
R1951 T3265 T3267 npadvmod month,old
R1952 T3266 T3265 punct -,month
R1953 T3267 T3269 amod old,mice
R1954 T3268 T3267 punct -,old
R1955 T3269 T3259 conj mice,mice
R1956 T3270 T3269 nmod Sam68,mice
R1957 T3271 T3270 punct −,Sam68
R1958 T3272 T3270 punct /,Sam68
R1959 T3273 T3270 punct −,Sam68
R1960 T3274 T3193 punct .,revealed
R1961 T3276 T3277 amod Similar,reductions
R1962 T3277 T3278 nsubjpass reductions,shown
R1963 T3279 T3277 prep in,reductions
R1964 T3280 T3281 amod trabecular,bone
R1965 T3281 T3279 pobj bone,in
R1966 T3282 T3278 auxpass were,shown
R1967 T3283 T3278 prep by,shown
R1968 T3284 T3283 pobj Faxitron,by
R1969 T3285 T3284 cc and,Faxitron
R1970 T3286 T3284 conj micro-CT,Faxitron
R1971 T3287 T3278 prep in,shown
R1972 T3288 T3289 det the,vertebrae
R1973 T3289 T3287 pobj vertebrae,in
R1974 T3290 T3289 amod fifth,vertebrae
R1975 T3291 T3289 compound lumbar,vertebrae
R1976 T3292 T3289 prep of,vertebrae
R1977 T3293 T3294 det the,mice
R1978 T3294 T3292 pobj mice,of
R1979 T3295 T3296 nummod 12,month
R1980 T3296 T3298 npadvmod month,old
R1981 T3297 T3296 punct -,month
R1982 T3298 T3294 amod old,mice
R1983 T3299 T3298 punct -,old
R1984 T3300 T3294 nmod Sam68,mice
R1985 T3301 T3300 punct +,Sam68
R1986 T3302 T3300 punct /,Sam68
R1987 T3303 T3300 punct +,Sam68
R1988 T3304 T3287 cc but,in
R1989 T3305 T3304 neg not,but
R1990 T3306 T3287 conj in,in
R1991 T3307 T3308 det the,mice
R1992 T3308 T3306 pobj mice,in
R1993 T3309 T3308 nmod Sam68,mice
R1994 T3310 T3309 punct −,Sam68
R1995 T3311 T3309 punct /,Sam68
R1996 T3312 T3309 punct −,Sam68
R1997 T3313 T3314 punct (,data
R1998 T3314 T3278 meta data,shown
R1999 T3315 T3314 amod unpublished,data
R2000 T3316 T3314 punct ),data
R2001 T3317 T3278 punct .,shown
R2002 T3319 T3320 amod Total,content
R2003 T3320 T3324 nsubj content,increased
R2004 T3321 T3320 compound body,content
R2005 T3322 T3320 compound bone,content
R2006 T3323 T3320 compound mineral,content
R2007 T3325 T3320 punct (,content
R2008 T3326 T3320 appos BMC,content
R2009 T3327 T3320 punct ),content
R2010 T3328 T3320 punct ", ",content
R2011 T3329 T3320 acl quantified,content
R2012 T3330 T3329 prep with,quantified
R2013 T3331 T3332 det a,densitometer
R2014 T3332 T3330 pobj densitometer,with
R2015 T3333 T3334 amod Lunar,PIXImus
R2016 T3334 T3332 compound PIXImus,densitometer
R2017 T3335 T3332 compound mouse,densitometer
R2018 T3336 T3337 punct (,Lunar
R2019 T3337 T3332 parataxis Lunar,densitometer
R2020 T3338 T3337 compound GE,Lunar
R2021 T3339 T3337 punct -,Lunar
R2022 T3340 T3337 punct ", ",Lunar
R2023 T3341 T3337 npadvmod Madison,Lunar
R2024 T3342 T3337 punct ", ",Lunar
R2025 T3343 T3337 npadvmod Wisconsin,Lunar
R2026 T3344 T3337 punct ", ",Lunar
R2027 T3345 T3346 compound United,States
R2028 T3346 T3337 npadvmod States,Lunar
R2029 T3347 T3337 punct ),Lunar
R2030 T3348 T3324 punct ", ",increased
R2031 T3349 T3324 prep in,increased
R2032 T3350 T3351 preconj both,Sam68
R2033 T3351 T3352 nmod Sam68,mice
R2034 T3352 T3349 pobj mice,in
R2035 T3353 T3351 punct +,Sam68
R2036 T3354 T3351 punct /,Sam68
R2037 T3355 T3351 punct +,Sam68
R2038 T3356 T3351 cc and,Sam68
R2039 T3357 T3351 conj Sam68,Sam68
R2040 T3358 T3357 punct −,Sam68
R2041 T3359 T3357 punct /,Sam68
R2042 T3360 T3357 punct −,Sam68
R2043 T3361 T3352 prep between,mice
R2044 T3362 T3363 nummod 4,months
R2045 T3363 T3361 pobj months,between
R2046 T3364 T3363 cc and,months
R2047 T3365 T3366 nummod 12,months
R2048 T3366 T3363 conj months,months
R2049 T3367 T3363 prep of,months
R2050 T3368 T3367 pobj age,of
R2051 T3369 T3324 punct ", ",increased
R2052 T3370 T3324 cc but,increased
R2053 T3371 T3372 advmod only,reached
R2054 T3372 T3324 conj reached,increased
R2055 T3373 T3372 dobj significance,reached
R2056 T3374 T3372 prep in,reached
R2057 T3375 T3376 det the,mice
R2058 T3376 T3374 pobj mice,in
R2059 T3377 T3376 nmod Sam68,mice
R2060 T3378 T3377 punct −,Sam68
R2061 T3379 T3377 punct /,Sam68
R2062 T3380 T3377 punct −,Sam68
R2063 T3381 T3382 punct (,Table
R2064 T3382 T3372 parataxis Table,reached
R2065 T3383 T3382 nummod 2,Table
R2066 T3384 T3382 punct ),Table
R2067 T3385 T3324 punct .,increased
R2068 T3387 T3388 advmod Similarly,seen
R2069 T3389 T3388 punct ", ",seen
R2070 T3390 T3391 det a,increase
R2071 T3391 T3388 nsubjpass increase,seen
R2072 T3392 T3391 amod greater,increase
R2073 T3393 T3391 prep in,increase
R2074 T3394 T3393 pobj BMC,in
R2075 T3395 T3388 auxpass was,seen
R2076 T3396 T3388 prep in,seen
R2077 T3397 T3398 det the,femur
R2078 T3398 T3396 pobj femur,in
R2079 T3399 T3398 cc and,femur
R2080 T3400 T3398 conj vertebra,femur
R2081 T3401 T3398 prep in,femur
R2082 T3402 T3403 det the,mice
R2083 T3403 T3401 pobj mice,in
R2084 T3404 T3403 nmod Sam68,mice
R2085 T3405 T3404 punct −,Sam68
R2086 T3406 T3404 punct /,Sam68
R2087 T3407 T3404 punct −,Sam68
R2088 T3408 T3388 punct .,seen
R2089 T3410 T3411 compound Bone,mineral
R2090 T3411 T3412 compound mineral,density
R2091 T3412 T3413 nsubj density,remained
R2092 T3414 T3412 punct (,density
R2093 T3415 T3412 appos BMD,density
R2094 T3416 T3413 punct ),remained
R2095 T3417 T3413 acomp constant,remained
R2096 T3418 T3413 cc or,remained
R2097 T3419 T3413 conj decreased,remained
R2098 T3420 T3413 prep in,remained
R2099 T3421 T3422 det the,mice
R2100 T3422 T3420 pobj mice,in
R2101 T3423 T3424 amod wild,type
R2102 T3424 T3422 compound type,mice
R2103 T3425 T3413 cc but,remained
R2104 T3426 T3413 conj increased,remained
R2105 T3427 T3426 advmod significantly,increased
R2106 T3428 T3426 prep in,increased
R2107 T3429 T3430 det the,body
R2108 T3430 T3428 pobj body,in
R2109 T3431 T3430 amod total,body
R2110 T3432 T3428 cc and,in
R2111 T3433 T3428 conj in,in
R2112 T3434 T3435 det the,regions
R2113 T3435 T3433 pobj regions,in
R2114 T3436 T3435 amod femoral,regions
R2115 T3437 T3436 cc and,femoral
R2116 T3438 T3436 conj vertebral,femoral
R2117 T3439 T3435 prep of,regions
R2118 T3440 T3439 pobj interest,of
R2119 T3441 T3426 prep in,increased
R2120 T3442 T3443 det the,Sam68
R2121 T3443 T3441 pobj Sam68,in
R2122 T3444 T3443 punct −,Sam68
R2123 T3445 T3443 punct /,Sam68
R2124 T3446 T3443 punct −,Sam68
R2125 T3447 T3448 punct (,Table
R2126 T3448 T3426 parataxis Table,increased
R2127 T3449 T3448 nummod 2,Table
R2128 T3450 T3448 punct ),Table
R2129 T3451 T3413 punct .,remained
R2130 T3453 T3454 det These,data
R2131 T3454 T3455 nsubj data,showed
R2132 T3456 T3457 mark that,continued
R2133 T3457 T3455 ccomp continued,showed
R2134 T3458 T3459 nmod Sam68,mice
R2135 T3459 T3457 nsubj mice,continued
R2136 T3460 T3458 punct −,Sam68
R2137 T3461 T3458 punct /,Sam68
R2138 T3462 T3458 punct −,Sam68
R2139 T3463 T3464 aux to,thrive
R2140 T3464 T3457 xcomp thrive,continued
R2141 T3465 T3464 cc and,thrive
R2142 T3466 T3464 conj accrue,thrive
R2143 T3467 T3466 dobj bone,accrue
R2144 T3468 T3466 prep in,accrue
R2145 T3469 T3470 det the,skeleton
R2146 T3470 T3468 pobj skeleton,in
R2147 T3471 T3470 amod axial,skeleton
R2148 T3472 T3471 cc and,axial
R2149 T3473 T3471 conj appendicular,axial
R2150 T3474 T3464 prep for,thrive
R2151 T3475 T3476 amod longer,12
R2152 T3476 T3478 nummod 12,months
R2153 T3477 T3476 quantmod than,12
R2154 T3478 T3474 pobj months,for
R2155 T3479 T3455 punct .,showed
R2156 T3481 T3482 nsubj This,was
R2157 T3483 T3482 prep in,was
R2158 T3484 T3483 pobj contrast,in
R2159 T3485 T3484 prep to,contrast
R2160 T3486 T3487 det the,situation
R2161 T3487 T3485 pobj situation,to
R2162 T3488 T3487 prep in,situation
R2163 T3489 T3490 npadvmod age,matched
R2164 T3490 T3492 amod matched,controls
R2165 T3491 T3490 punct -,matched
R2166 T3492 T3488 pobj controls,in
R2167 T3493 T3492 punct ", ",controls
R2168 T3494 T3492 compound littermate,controls
R2169 T3495 T3496 prep in,lost
R2170 T3496 T3487 relcl lost,situation
R2171 T3497 T3495 pobj which,in
R2172 T3498 T3499 det a,amount
R2173 T3499 T3496 nsubjpass amount,lost
R2174 T3500 T3499 amod significant,amount
R2175 T3501 T3499 prep of,amount
R2176 T3502 T3501 pobj bone,of
R2177 T3503 T3496 auxpass was,lost
R2178 T3504 T3496 prep over,lost
R2179 T3505 T3506 det the,timeframe
R2180 T3506 T3504 pobj timeframe,over
R2181 T3507 T3506 amod same,timeframe
R2182 T3508 T3482 punct .,was
R2183 T3587 T3588 advmod Three,Dimensional
R2184 T3588 T3590 amod Dimensional,Architecture
R2185 T3589 T3588 punct -,Dimensional
R2186 T3590 T3591 nsubjpass Architecture,Preserved
R2187 T3592 T3590 prep of,Architecture
R2188 T3593 T3592 pobj Bone,of
R2189 T3594 T3591 auxpass Is,Preserved
R2190 T3595 T3591 prep in,Preserved
R2191 T3596 T3597 amod Aged,Mice
R2192 T3597 T3595 pobj Mice,in
R2193 T3598 T3597 nmod Sam68,Mice
R2194 T3599 T3598 punct −,Sam68
R2195 T3600 T3598 punct /,Sam68
R2196 T3601 T3598 punct −,Sam68
R2197 T3603 T3604 compound Bone,loss
R2198 T3604 T3605 nsubj loss,are
R2199 T3606 T3604 cc and,loss
R2200 T3607 T3608 amod compromised,architecture
R2201 T3608 T3604 conj architecture,loss
R2202 T3609 T3610 amod characteristic,features
R2203 T3610 T3605 attr features,are
R2204 T3611 T3610 prep of,features
R2205 T3612 T3613 det the,skeletons
R2206 T3613 T3611 pobj skeletons,of
R2207 T3614 T3613 prep of,skeletons
R2208 T3615 T3616 amod aged,mice
R2209 T3616 T3614 pobj mice,of
R2210 T3617 T3616 nmod C57BL,mice
R2211 T3618 T3617 punct /,C57BL
R2212 T3619 T3617 nummod 6,C57BL
R2213 T3620 T3605 cc and,are
R2214 T3621 T3605 conj resemble,are
R2215 T3622 T3623 det the,features
R2216 T3623 T3621 dobj features,resemble
R2217 T3624 T3623 amod clinical,features
R2218 T3625 T3623 prep of,features
R2219 T3626 T3627 npadvmod age,related
R2220 T3627 T3629 amod related,loss
R2221 T3628 T3627 punct -,related
R2222 T3629 T3625 pobj loss,of
R2223 T3630 T3629 compound bone,loss
R2224 T3631 T3629 prep in,loss
R2225 T3632 T3631 pobj humans,in
R2226 T3633 T3634 dep that,predispose
R2227 T3634 T3623 relcl predispose,features
R2228 T3635 T3634 aux can,predispose
R2229 T3636 T3637 det an,individual
R2230 T3637 T3634 dobj individual,predispose
R2231 T3638 T3634 prep to,predispose
R2232 T3639 T3638 pobj fracture,to
R2233 T3640 T3605 punct .,are
R2234 T3642 T3643 nsubj Quantification,confirmed
R2235 T3644 T3642 prep of,Quantification
R2236 T3645 T3646 det the,data
R2237 T3646 T3644 pobj data,of
R2238 T3647 T3646 compound micro-CT,data
R2239 T3648 T3646 acl shown,data
R2240 T3649 T3648 prep in,shown
R2241 T3650 T3651 compound Figure,3B
R2242 T3651 T3649 pobj 3B,in
R2243 T3652 T3653 det the,reduction
R2244 T3653 T3643 dobj reduction,confirmed
R2245 T3654 T3653 prep in,reduction
R2246 T3655 T3656 compound bone,volume
R2247 T3656 T3654 pobj volume,in
R2248 T3657 T3653 prep compared,reduction
R2249 T3658 T3657 prep with,compared
R2250 T3659 T3660 compound tissue,volume
R2251 T3660 T3658 pobj volume,with
R2252 T3661 T3662 punct (,4A
R2253 T3662 T3660 parataxis 4A,volume
R2254 T3663 T3664 compound BV,TV
R2255 T3664 T3662 dep TV,4A
R2256 T3665 T3664 punct /,TV
R2257 T3666 T3662 punct ;,4A
R2258 T3667 T3662 compound Figure,4A
R2259 T3668 T3662 punct ", ",4A
R2260 T3669 T3662 amod left,4A
R2261 T3670 T3662 punct ),4A
R2262 T3671 T3660 prep in,volume
R2263 T3672 T3673 det the,mice
R2264 T3673 T3671 pobj mice,in
R2265 T3674 T3675 nummod 12,month
R2266 T3675 T3677 npadvmod month,old
R2267 T3676 T3675 punct -,month
R2268 T3677 T3673 amod old,mice
R2269 T3678 T3677 punct -,old
R2270 T3679 T3673 nmod Sam68,mice
R2271 T3680 T3679 punct +,Sam68
R2272 T3681 T3679 punct /,Sam68
R2273 T3682 T3679 punct +,Sam68
R2274 T3683 T3684 punct (,bars
R2275 T3684 T3673 parataxis bars,mice
R2276 T3685 T3684 amod hatched,bars
R2277 T3686 T3684 punct ),bars
R2278 T3687 T3660 prep compared,volume
R2279 T3688 T3687 prep with,compared
R2280 T3689 T3690 nummod 4,month
R2281 T3690 T3692 npadvmod month,old
R2282 T3691 T3690 punct -,month
R2283 T3692 T3694 amod old,mice
R2284 T3693 T3692 punct -,old
R2285 T3694 T3688 pobj mice,with
R2286 T3695 T3694 prep of,mice
R2287 T3696 T3697 det both,genotypes
R2288 T3697 T3695 pobj genotypes,of
R2289 T3698 T3699 punct (,bars
R2290 T3699 T3694 parataxis bars,mice
R2291 T3700 T3699 amod solid,bars
R2292 T3701 T3699 punct ),bars
R2293 T3702 T3694 cc and,mice
R2294 T3703 T3704 nummod 12,month
R2295 T3704 T3706 npadvmod month,old
R2296 T3705 T3704 punct -,month
R2297 T3706 T3708 amod old,mice
R2298 T3707 T3706 punct -,old
R2299 T3708 T3694 conj mice,mice
R2300 T3709 T3708 nmod Sam68,mice
R2301 T3710 T3709 punct −,Sam68
R2302 T3711 T3709 punct /,Sam68
R2303 T3712 T3709 punct −,Sam68
R2304 T3713 T3714 punct (,bars
R2305 T3714 T3708 parataxis bars,mice
R2306 T3715 T3714 amod stippled,bars
R2307 T3716 T3714 punct ),bars
R2308 T3717 T3643 punct .,confirmed
R2309 T3719 T3720 nsubjpass This,associated
R2310 T3721 T3720 auxpass was,associated
R2311 T3722 T3720 prep with,associated
R2312 T3723 T3724 det a,increase
R2313 T3724 T3722 pobj increase,with
R2314 T3725 T3724 amod significant,increase
R2315 T3726 T3727 punct (,denoted
R2316 T3727 T3724 parataxis denoted,increase
R2317 T3728 T3729 nsubj p,0.01
R2318 T3729 T3727 ccomp 0.01,denoted
R2319 T3730 T3729 punct <,0.01
R2320 T3731 T3727 punct ;,denoted
R2321 T3732 T3727 agent by,denoted
R2322 T3733 T3734 det the,asterisks
R2323 T3734 T3732 pobj asterisks,by
R2324 T3735 T3727 punct ),denoted
R2325 T3736 T3724 prep in,increase
R2326 T3737 T3738 det the,index
R2327 T3738 T3736 pobj index,in
R2328 T3739 T3738 compound structure,index
R2329 T3740 T3738 compound model,index
R2330 T3741 T3738 punct ", ",index
R2331 T3742 T3743 dep which,measures
R2332 T3743 T3738 relcl measures,index
R2333 T3744 T3745 det the,ratio
R2334 T3745 T3743 dobj ratio,measures
R2335 T3746 T3745 prep of,ratio
R2336 T3747 T3748 npadvmod plate,like
R2337 T3748 T3746 pobj like,of
R2338 T3749 T3748 punct -,like
R2339 T3750 T3748 prep to,like
R2340 T3751 T3752 npadvmod rod,like
R2341 T3752 T3750 pobj like,to
R2342 T3753 T3752 punct -,like
R2343 T3754 T3748 appos structures,like
R2344 T3755 T3756 punct (,4A
R2345 T3756 T3743 parataxis 4A,measures
R2346 T3757 T3756 compound Figure,4A
R2347 T3758 T3756 punct ", ",4A
R2348 T3759 T3756 appos right,4A
R2349 T3760 T3756 punct ),4A
R2350 T3761 T3720 punct .,associated
R2351 T3763 T3764 det A,increase
R2352 T3764 T3766 nsubj increase,was
R2353 T3765 T3764 amod quantifiable,increase
R2354 T3767 T3764 prep in,increase
R2355 T3768 T3769 det the,separation
R2356 T3769 T3767 pobj separation,in
R2357 T3770 T3769 nmod mean,separation
R2358 T3771 T3769 amod trabecular,separation
R2359 T3772 T3769 punct (,separation
R2360 T3773 T3769 appos Tb.Sp,separation
R2361 T3774 T3769 punct ),separation
R2362 T3775 T3776 punct (,data
R2363 T3776 T3769 parataxis data,separation
R2364 T3777 T3776 amod unpublished,data
R2365 T3778 T3776 punct ),data
R2366 T3779 T3764 prep in,increase
R2367 T3780 T3781 nummod 12,month
R2368 T3781 T3783 npadvmod month,old
R2369 T3782 T3781 punct -,month
R2370 T3783 T3785 amod old,mice
R2371 T3784 T3783 punct -,old
R2372 T3785 T3779 pobj mice,in
R2373 T3786 T3785 nmod Sam68,mice
R2374 T3787 T3786 punct +,Sam68
R2375 T3788 T3786 punct /,Sam68
R2376 T3789 T3786 punct +,Sam68
R2377 T3790 T3766 prep due,was
R2378 T3791 T3790 pcomp to,due
R2379 T3792 T3793 det an,increase
R2380 T3793 T3790 pobj increase,due
R2381 T3794 T3793 prep in,increase
R2382 T3795 T3796 det the,percentage
R2383 T3796 T3794 pobj percentage,in
R2384 T3797 T3796 prep of,percentage
R2385 T3798 T3797 pobj spaces,of
R2386 T3799 T3798 acl falling,spaces
R2387 T3800 T3799 prep in,falling
R2388 T3801 T3802 det the,range
R2389 T3802 T3800 pobj range,in
R2390 T3803 T3802 prep of,range
R2391 T3804 T3805 quantmod 350,700
R2392 T3805 T3807 nummod 700,μm
R2393 T3806 T3805 quantmod to,700
R2394 T3807 T3803 pobj μm,of
R2395 T3808 T3809 punct (,4B
R2396 T3809 T3766 parataxis 4B,was
R2397 T3810 T3809 compound Figure,4B
R2398 T3811 T3809 punct ", ",4B
R2399 T3812 T3813 amod red,line
R2400 T3813 T3809 appos line,4B
R2401 T3814 T3813 amod hatched,line
R2402 T3815 T3809 punct ),4B
R2403 T3816 T3766 punct .,was
R2404 T3818 T3819 amod Trabecular,thickness
R2405 T3819 T3820 nsubj thickness,remained
R2406 T3821 T3820 acomp constant,remained
R2407 T3822 T3820 prep among,remained
R2408 T3823 T3824 det the,groups
R2409 T3824 T3822 pobj groups,among
R2410 T3825 T3824 amod different,groups
R2411 T3826 T3824 prep of,groups
R2412 T3827 T3826 pobj mice,of
R2413 T3828 T3829 punct (,data
R2414 T3829 T3820 meta data,remained
R2415 T3830 T3829 amod unpublished,data
R2416 T3831 T3829 punct ),data
R2417 T3832 T3820 punct .,remained
R2418 T3834 T3835 prep In,meant
R2419 T3836 T3834 pobj effect,In
R2420 T3837 T3835 punct ", ",meant
R2421 T3838 T3835 nsubj this,meant
R2422 T3839 T3840 mark that,were
R2423 T3840 T3835 ccomp were,meant
R2424 T3841 T3840 expl there,were
R2425 T3842 T3843 amod fewer,trabeculae
R2426 T3843 T3840 attr trabeculae,were
R2427 T3844 T3843 punct ", ",trabeculae
R2428 T3845 T3846 advmod rather,than
R2429 T3846 T3843 cc than,trabeculae
R2430 T3847 T3848 amod equivalent,numbers
R2431 T3848 T3843 conj numbers,trabeculae
R2432 T3849 T3848 prep of,numbers
R2433 T3850 T3851 amod thin,trabeculae
R2434 T3851 T3849 pobj trabeculae,of
R2435 T3852 T3843 punct ", ",trabeculae
R2436 T3853 T3843 prep in,trabeculae
R2437 T3854 T3855 det the,mice
R2438 T3855 T3853 pobj mice,in
R2439 T3856 T3857 nummod 12,month
R2440 T3857 T3859 npadvmod month,old
R2441 T3858 T3857 punct -,month
R2442 T3859 T3855 amod old,mice
R2443 T3860 T3859 punct -,old
R2444 T3861 T3855 nmod Sam68,mice
R2445 T3862 T3861 punct +,Sam68
R2446 T3863 T3861 punct /,Sam68
R2447 T3864 T3861 punct +,Sam68
R2448 T3865 T3843 prep compared,trabeculae
R2449 T3866 T3865 prep with,compared
R2450 T3867 T3866 pobj any,with
R2451 T3868 T3867 prep of,any
R2452 T3869 T3870 det the,groups
R2453 T3870 T3868 pobj groups,of
R2454 T3871 T3870 amod other,groups
R2455 T3872 T3870 prep of,groups
R2456 T3873 T3872 pobj mice,of
R2457 T3874 T3835 punct .,meant
R2458 T4163 T4164 compound Bone,Remodeling
R2459 T4164 T4165 nsubjpass Remodeling,Preserved
R2460 T4166 T4165 auxpass Is,Preserved
R2461 T4167 T4165 prep in,Preserved
R2462 T4168 T4169 amod Aged,Mice
R2463 T4169 T4167 pobj Mice,in
R2464 T4170 T4169 nmod Sam68,Mice
R2465 T4171 T4170 punct −,Sam68
R2466 T4172 T4170 punct /,Sam68
R2467 T4173 T4170 punct −,Sam68
R2468 T4175 T4176 aux To,define
R2469 T4176 T4178 advcl define,prepared
R2470 T4177 T4176 advmod further,define
R2471 T4179 T4180 det the,mechanisms
R2472 T4180 T4176 dobj mechanisms,define
R2473 T4181 T4180 acl involved,mechanisms
R2474 T4182 T4181 prep in,involved
R2475 T4183 T4184 det the,preservation
R2476 T4184 T4182 pobj preservation,in
R2477 T4185 T4184 prep of,preservation
R2478 T4186 T4187 compound bone,mass
R2479 T4187 T4185 pobj mass,of
R2480 T4188 T4184 prep in,preservation
R2481 T4189 T4190 amod aged,mice
R2482 T4190 T4188 pobj mice,in
R2483 T4191 T4190 nmod Sam68,mice
R2484 T4192 T4191 punct −,Sam68
R2485 T4193 T4191 punct /,Sam68
R2486 T4194 T4191 punct −,Sam68
R2487 T4195 T4178 punct ", ",prepared
R2488 T4196 T4178 nsubj we,prepared
R2489 T4197 T4178 dobj sections,prepared
R2490 T4198 T4178 prep from,prepared
R2491 T4199 T4200 npadvmod plastic,embedded
R2492 T4200 T4202 amod embedded,femora
R2493 T4201 T4200 punct -,embedded
R2494 T4202 T4198 pobj femora,from
R2495 T4203 T4202 cc and,femora
R2496 T4204 T4202 conj tibia,femora
R2497 T4205 T4206 aux to,evaluate
R2498 T4206 T4178 advcl evaluate,prepared
R2499 T4207 T4208 nmod osteoblast,activity
R2500 T4208 T4206 dobj activity,evaluate
R2501 T4209 T4207 cc and,osteoblast
R2502 T4210 T4207 conj osteoclast,osteoblast
R2503 T4211 T4212 punct (,Figure
R2504 T4212 T4178 parataxis Figure,prepared
R2505 T4213 T4212 nummod 5,Figure
R2506 T4214 T4212 punct ),Figure
R2507 T4215 T4178 punct .,prepared
R2508 T4217 T4218 advmod In,situ
R2509 T4218 T4219 amod situ,staining
R2510 T4219 T4222 nsubjpass staining,used
R2511 T4220 T4219 nmod enzyme,staining
R2512 T4221 T4219 amod histochemical,staining
R2513 T4223 T4219 prep for,staining
R2514 T4224 T4225 nmod alkaline,phosphatase
R2515 T4225 T4226 nmod phosphatase,activity
R2516 T4226 T4223 pobj activity,for
R2517 T4227 T4225 punct (,phosphatase
R2518 T4228 T4225 appos ALP,phosphatase
R2519 T4229 T4230 punct ;,stain
R2520 T4230 T4228 parataxis stain,ALP
R2521 T4231 T4230 amod brown,stain
R2522 T4232 T4230 punct ),stain
R2523 T4233 T4222 auxpass was,used
R2524 T4234 T4222 prep as,used
R2525 T4235 T4236 det a,biomarker
R2526 T4236 T4234 pobj biomarker,as
R2527 T4237 T4236 prep for,biomarker
R2528 T4238 T4237 pobj osteoblasts,for
R2529 T4239 T4238 cc and,osteoblasts
R2530 T4240 T4241 npadvmod tartrate,resistant
R2531 T4241 T4243 amod resistant,activity
R2532 T4242 T4241 punct -,resistant
R2533 T4243 T4238 conj activity,osteoblasts
R2534 T4244 T4243 nmod ALP,activity
R2535 T4245 T4246 punct (,stain
R2536 T4246 T4243 parataxis stain,activity
R2537 T4247 T4248 npadvmod tartrate,resistant
R2538 T4248 T4250 amod resistant,phosphatase
R2539 T4249 T4248 punct -,resistant
R2540 T4250 T4246 dep phosphatase,stain
R2541 T4251 T4250 compound acid,phosphatase
R2542 T4252 T4250 punct [,phosphatase
R2543 T4253 T4250 appos TRAP,phosphatase
R2544 T4254 T4246 punct ],stain
R2545 T4255 T4246 punct ;,stain
R2546 T4256 T4246 amod red,stain
R2547 T4257 T4246 punct ),stain
R2548 T4258 T4222 prep as,used
R2549 T4259 T4260 det a,marker
R2550 T4260 T4258 pobj marker,as
R2551 T4261 T4260 prep for,marker
R2552 T4262 T4261 pobj osteoclasts,for
R2553 T4263 T4222 punct .,used
R2554 T4265 T4266 amod Little,difference
R2555 T4266 T4267 nsubjpass difference,seen
R2556 T4268 T4267 auxpass was,seen
R2557 T4269 T4267 prep between,seen
R2558 T4270 T4271 nmod Sam68,mice
R2559 T4271 T4269 pobj mice,between
R2560 T4272 T4270 punct +,Sam68
R2561 T4273 T4270 punct /,Sam68
R2562 T4274 T4270 punct +,Sam68
R2563 T4275 T4276 punct (,5A
R2564 T4276 T4270 parataxis 5A,Sam68
R2565 T4277 T4276 compound Figure,5A
R2566 T4278 T4276 cc and,5A
R2567 T4279 T4276 conj 5B,5A
R2568 T4280 T4276 punct ),5A
R2569 T4281 T4270 cc and,Sam68
R2570 T4282 T4270 conj Sam68,Sam68
R2571 T4283 T4282 punct −,Sam68
R2572 T4284 T4282 punct /,Sam68
R2573 T4285 T4282 punct −,Sam68
R2574 T4286 T4287 punct (,5C
R2575 T4287 T4282 parataxis 5C,Sam68
R2576 T4288 T4287 compound Figure,5C
R2577 T4289 T4287 cc and,5C
R2578 T4290 T4287 conj 5D,5C
R2579 T4291 T4287 punct ),5C
R2580 T4292 T4267 prep at,seen
R2581 T4293 T4294 nummod 4,months
R2582 T4294 T4292 pobj months,at
R2583 T4295 T4294 prep of,months
R2584 T4296 T4295 pobj age,of
R2585 T4297 T4267 punct .,seen
R2586 T4299 T4300 npadvmod ALP,positive
R2587 T4300 T4305 amod positive,cells
R2588 T4301 T4299 punct -,ALP
R2589 T4302 T4299 cc and,ALP
R2590 T4303 T4299 conj TRAP,ALP
R2591 T4304 T4300 punct -,positive
R2592 T4305 T4306 nsubjpass cells,reduced
R2593 T4307 T4306 auxpass were,reduced
R2594 T4308 T4306 prep in,reduced
R2595 T4309 T4310 det the,mice
R2596 T4310 T4308 pobj mice,in
R2597 T4311 T4312 nummod 12,month
R2598 T4312 T4314 npadvmod month,old
R2599 T4313 T4312 punct -,month
R2600 T4314 T4310 amod old,mice
R2601 T4315 T4314 punct -,old
R2602 T4316 T4310 nmod Sam68,mice
R2603 T4317 T4316 punct +,Sam68
R2604 T4318 T4316 punct /,Sam68
R2605 T4319 T4316 punct +,Sam68
R2606 T4320 T4321 punct (,5E
R2607 T4321 T4306 parataxis 5E,reduced
R2608 T4322 T4321 compound Figure,5E
R2609 T4323 T4321 cc and,5E
R2610 T4324 T4321 conj 5F,5E
R2611 T4325 T4321 punct ),5E
R2612 T4326 T4306 cc and,reduced
R2613 T4327 T4306 conj remained,reduced
R2614 T4328 T4327 acomp unchanged,remained
R2615 T4329 T4327 prep in,remained
R2616 T4330 T4331 nummod 12,month
R2617 T4331 T4333 npadvmod month,old
R2618 T4332 T4331 punct -,month
R2619 T4333 T4335 amod old,mice
R2620 T4334 T4333 punct -,old
R2621 T4335 T4329 pobj mice,in
R2622 T4336 T4335 nmod Sam68,mice
R2623 T4337 T4336 punct −,Sam68
R2624 T4338 T4336 punct /,Sam68
R2625 T4339 T4336 punct −,Sam68
R2626 T4340 T4341 punct (,5G
R2627 T4341 T4327 parataxis 5G,remained
R2628 T4342 T4341 compound Figure,5G
R2629 T4343 T4341 cc and,5G
R2630 T4344 T4341 conj 5H,5G
R2631 T4345 T4341 punct ),5G
R2632 T4346 T4306 punct .,reduced
R2633 T4348 T4349 det The,reduction
R2634 T4349 T4350 nsubj reduction,argued
R2635 T4351 T4349 prep in,reduction
R2636 T4352 T4353 preconj both,osteoblast
R2637 T4353 T4354 nmod osteoblast,activity
R2638 T4354 T4351 pobj activity,in
R2639 T4355 T4353 cc and,osteoblast
R2640 T4356 T4353 conj osteoclast,osteoblast
R2641 T4357 T4349 prep in,reduction
R2642 T4358 T4359 det the,mice
R2643 T4359 T4357 pobj mice,in
R2644 T4360 T4361 nummod 12,month
R2645 T4361 T4363 npadvmod month,old
R2646 T4362 T4361 punct -,month
R2647 T4363 T4359 amod old,mice
R2648 T4364 T4363 punct -,old
R2649 T4365 T4359 nmod Sam68,mice
R2650 T4366 T4365 punct +,Sam68
R2651 T4367 T4365 punct /,Sam68
R2652 T4368 T4365 punct +,Sam68
R2653 T4369 T4350 prep against,argued
R2654 T4370 T4371 nsubjpass bone,lost
R2655 T4371 T4369 pcomp lost,against
R2656 T4372 T4371 auxpass being,lost
R2657 T4373 T4374 advmod primarily,due
R2658 T4374 T4371 prep due,lost
R2659 T4375 T4374 pcomp to,due
R2660 T4376 T4377 det a,increase
R2661 T4377 T4374 pobj increase,due
R2662 T4378 T4377 amod relative,increase
R2663 T4379 T4377 prep in,increase
R2664 T4380 T4379 pobj osteoclast,in
R2665 T4381 T4380 prep over,osteoclast
R2666 T4382 T4381 pobj osteoblast,over
R2667 T4383 T4380 appos activity,osteoclast
R2668 T4384 T4371 punct ", ",lost
R2669 T4385 T4386 mark as,seen
R2670 T4386 T4371 advcl seen,lost
R2671 T4387 T4386 prep in,seen
R2672 T4388 T4389 amod high,turnover
R2673 T4389 T4390 compound turnover,disease
R2674 T4390 T4387 pobj disease,in
R2675 T4391 T4392 punct [,49
R2676 T4392 T4350 parataxis 49,argued
R2677 T4393 T4392 punct ],49
R2678 T4394 T4350 punct .,argued
R2679 T4396 T4397 amod Histomorphometric,analyses
R2680 T4397 T4398 nsubj analyses,corroborated
R2681 T4399 T4400 punct [,50
R2682 T4400 T4397 parataxis 50,analyses
R2683 T4401 T4400 punct ],50
R2684 T4402 T4397 prep of,analyses
R2685 T4403 T4404 det the,bones
R2686 T4404 T4402 pobj bones,of
R2687 T4405 T4404 amod long,bones
R2688 T4406 T4407 punct (,Table
R2689 T4407 T4397 parataxis Table,analyses
R2690 T4408 T4407 nummod 3,Table
R2691 T4409 T4407 punct ),Table
R2692 T4410 T4411 det the,evidence
R2693 T4411 T4398 dobj evidence,corroborated
R2694 T4412 T4411 amod radiologic,evidence
R2695 T4413 T4411 prep of,evidence
R2696 T4414 T4415 npadvmod age,related
R2697 T4415 T4417 amod related,loss
R2698 T4416 T4415 punct -,related
R2699 T4417 T4413 pobj loss,of
R2700 T4418 T4417 compound bone,loss
R2701 T4419 T4398 punct .,corroborated
R2702 T4421 T4422 compound Bone,volume
R2703 T4422 T4423 nsubjpass volume,reduced
R2704 T4424 T4422 punct (,volume
R2705 T4425 T4426 compound BV,TV
R2706 T4426 T4422 appos TV,volume
R2707 T4427 T4426 punct /,TV
R2708 T4428 T4422 punct ),volume
R2709 T4429 T4422 punct ", ",volume
R2710 T4430 T4431 advmod newly,formed
R2711 T4431 T4432 amod formed,osteoid
R2712 T4432 T4422 conj osteoid,volume
R2713 T4433 T4432 punct (,osteoid
R2714 T4434 T4435 compound OV,TV
R2715 T4435 T4432 appos TV,osteoid
R2716 T4436 T4435 punct /,TV
R2717 T4437 T4432 punct ),osteoid
R2718 T4438 T4432 punct ", ",osteoid
R2719 T4439 T4432 cc and,osteoid
R2720 T4440 T4441 compound mineral,rate
R2721 T4441 T4432 conj rate,osteoid
R2722 T4442 T4441 compound apposition,rate
R2723 T4443 T4441 punct (,rate
R2724 T4444 T4441 appos MAR,rate
R2725 T4445 T4423 punct ),reduced
R2726 T4446 T4423 auxpass were,reduced
R2727 T4447 T4423 dep all,reduced
R2728 T4448 T4423 advmod significantly,reduced
R2729 T4449 T4423 prep in,reduced
R2730 T4450 T4451 nummod 12,month
R2731 T4451 T4453 npadvmod month,old
R2732 T4452 T4451 punct -,month
R2733 T4453 T4455 amod old,mice
R2734 T4454 T4453 punct -,old
R2735 T4455 T4449 pobj mice,in
R2736 T4456 T4455 nmod Sam68,mice
R2737 T4457 T4456 punct +,Sam68
R2738 T4458 T4456 punct /,Sam68
R2739 T4459 T4456 punct +,Sam68
R2740 T4460 T4423 prep compared,reduced
R2741 T4461 T4460 prep with,compared
R2742 T4462 T4463 nummod 4,month
R2743 T4463 T4465 npadvmod month,old
R2744 T4464 T4463 punct -,month
R2745 T4465 T4467 amod old,mice
R2746 T4466 T4465 punct -,old
R2747 T4467 T4461 pobj mice,with
R2748 T4468 T4467 nmod Sam68,mice
R2749 T4469 T4468 punct +,Sam68
R2750 T4470 T4468 punct /,Sam68
R2751 T4471 T4468 punct +,Sam68
R2752 T4472 T4423 punct .,reduced
R2753 T4474 T4475 det These,reductions
R2754 T4475 T4476 nsubjpass reductions,associated
R2755 T4477 T4476 auxpass were,associated
R2756 T4478 T4476 prep with,associated
R2757 T4479 T4480 det a,increase
R2758 T4480 T4478 pobj increase,with
R2759 T4481 T4480 amod significant,increase
R2760 T4482 T4480 prep in,increase
R2761 T4483 T4484 compound marrow,fat
R2762 T4484 T4482 pobj fat,in
R2763 T4485 T4486 punct (,TV
R2764 T4486 T4480 parataxis TV,increase
R2765 T4487 T4486 compound FV,TV
R2766 T4488 T4486 punct /,TV
R2767 T4489 T4486 punct ),TV
R2768 T4490 T4480 cc and,increase
R2769 T4491 T4492 amod decreased,numbers
R2770 T4492 T4480 conj numbers,increase
R2771 T4493 T4492 prep of,numbers
R2772 T4494 T4493 pobj osteoblasts,of
R2773 T4495 T4496 punct (,TV
R2774 T4496 T4494 parataxis TV,osteoblasts
R2775 T4497 T4496 compound nOB,TV
R2776 T4498 T4496 punct /,TV
R2777 T4499 T4496 punct ),TV
R2778 T4500 T4494 cc and,osteoblasts
R2779 T4501 T4494 conj osteoclasts,osteoblasts
R2780 T4502 T4503 punct (,TV
R2781 T4503 T4501 parataxis TV,osteoclasts
R2782 T4504 T4503 compound nOC,TV
R2783 T4505 T4503 punct /,TV
R2784 T4506 T4503 punct ),TV
R2785 T4507 T4492 prep per,numbers
R2786 T4508 T4509 compound tissue,volume
R2787 T4509 T4507 pobj volume,per
R2788 T4510 T4511 punct (,Table
R2789 T4511 T4476 parataxis Table,associated
R2790 T4512 T4511 nummod 3,Table
R2791 T4513 T4511 punct ),Table
R2792 T4514 T4476 punct .,associated
R2793 T4516 T4517 det These,results
R2794 T4517 T4518 nsubj results,were
R2795 T4519 T4518 prep in,were
R2796 T4520 T4519 pobj contrast,in
R2797 T4521 T4520 prep to,contrast
R2798 T4522 T4521 pobj those,to
R2799 T4523 T4522 prep of,those
R2800 T4524 T4525 det the,mice
R2801 T4525 T4523 pobj mice,of
R2802 T4526 T4527 nummod 12,month
R2803 T4527 T4529 npadvmod month,old
R2804 T4528 T4527 punct -,month
R2805 T4529 T4525 amod old,mice
R2806 T4530 T4529 punct -,old
R2807 T4531 T4525 nmod Sam68,mice
R2808 T4532 T4531 punct −,Sam68
R2809 T4533 T4531 punct /,Sam68
R2810 T4534 T4531 punct −,Sam68
R2811 T4535 T4536 prep in,resembled
R2812 T4536 T4522 relcl resembled,those
R2813 T4537 T4535 pobj which,in
R2814 T4538 T4539 det all,parameters
R2815 T4539 T4536 nsubj parameters,resembled
R2816 T4540 T4539 amod histomorphometric,parameters
R2817 T4541 T4539 punct ", ",parameters
R2818 T4542 T4539 prep including,parameters
R2819 T4543 T4544 compound marrow,fat
R2820 T4544 T4542 pobj fat,including
R2821 T4545 T4536 punct ", ",resembled
R2822 T4546 T4536 dobj those,resembled
R2823 T4547 T4546 prep of,those
R2824 T4548 T4549 amod young,mice
R2825 T4549 T4547 pobj mice,of
R2826 T4550 T4549 prep of,mice
R2827 T4551 T4552 preconj either,genotype
R2828 T4552 T4550 pobj genotype,of
R2829 T4553 T4554 punct (,Table
R2830 T4554 T4536 parataxis Table,resembled
R2831 T4555 T4554 nummod 3,Table
R2832 T4556 T4554 punct ),Table
R2833 T4557 T4518 punct .,were
R2834 T4559 T4560 advmod When,expressed
R2835 T4560 T4563 advcl expressed,were
R2836 T4561 T4560 nsubjpass cells,expressed
R2837 T4562 T4560 auxpass were,expressed
R2838 T4564 T4560 prep as,expressed
R2839 T4565 T4566 det a,function
R2840 T4566 T4564 pobj function,as
R2841 T4567 T4566 prep of,function
R2842 T4568 T4569 det the,perimeter
R2843 T4569 T4567 pobj perimeter,of
R2844 T4570 T4569 compound bone,perimeter
R2845 T4571 T4572 punct (,BP
R2846 T4572 T4560 parataxis BP,expressed
R2847 T4573 T4572 compound nOB,BP
R2848 T4574 T4572 punct /,BP
R2849 T4575 T4572 punct ", ",BP
R2850 T4576 T4577 compound nOC,BP
R2851 T4577 T4572 appos BP,BP
R2852 T4578 T4577 punct /,BP
R2853 T4579 T4572 punct ),BP
R2854 T4580 T4563 punct ", ",were
R2855 T4581 T4563 expl there,were
R2856 T4582 T4583 det no,differences
R2857 T4583 T4563 attr differences,were
R2858 T4584 T4583 amod statistical,differences
R2859 T4585 T4563 cc and,were
R2860 T4586 T4587 det the,ratio
R2861 T4587 T4588 nsubj ratio,was
R2862 T4588 T4563 conj was,were
R2863 T4589 T4587 prep of,ratio
R2864 T4590 T4589 pobj osteoblasts,of
R2865 T4591 T4590 prep to,osteoblasts
R2866 T4592 T4591 pobj osteoclasts,to
R2867 T4593 T4588 acomp similar,was
R2868 T4594 T4588 prep in,was
R2869 T4595 T4596 det all,groups
R2870 T4596 T4594 pobj groups,in
R2871 T4597 T4596 prep of,groups
R2872 T4598 T4597 pobj mice,of
R2873 T4599 T4600 punct (,Table
R2874 T4600 T4588 parataxis Table,was
R2875 T4601 T4600 nummod 3,Table
R2876 T4602 T4600 punct ),Table
R2877 T4603 T4563 punct .,were
R2878 T4605 T4606 amod Circulating,levels
R2879 T4606 T4607 nsubj levels,showed
R2880 T4608 T4606 prep of,levels
R2881 T4609 T4610 compound serum,telopeptide
R2882 T4610 T4608 pobj telopeptide,of
R2883 T4611 T4610 compound C,telopeptide
R2884 T4612 T4610 punct -,telopeptide
R2885 T4613 T4614 punct (,Diagnostics
R2886 T4614 T4610 parataxis Diagnostics,telopeptide
R2887 T4615 T4614 dep sCTX,Diagnostics
R2888 T4616 T4614 punct ;,Diagnostics
R2889 T4617 T4614 compound Roche,Diagnostics
R2890 T4618 T4614 punct ", ",Diagnostics
R2891 T4619 T4614 npadvmod Mannheim,Diagnostics
R2892 T4620 T4614 punct ", ",Diagnostics
R2893 T4621 T4614 npadvmod Germany,Diagnostics
R2894 T4622 T4614 punct ),Diagnostics
R2895 T4623 T4610 cc and,telopeptide
R2896 T4624 T4610 conj ALP,telopeptide
R2897 T4625 T4626 amod little,difference
R2898 T4626 T4607 dobj difference,showed
R2899 T4627 T4607 prep among,showed
R2900 T4628 T4629 det the,groups
R2901 T4629 T4627 pobj groups,among
R2902 T4630 T4607 punct ", ",showed
R2903 T4631 T4607 prep except,showed
R2904 T4632 T4631 prep for,except
R2905 T4633 T4634 det a,decrease
R2906 T4634 T4632 pobj decrease,for
R2907 T4635 T4634 amod small,decrease
R2908 T4636 T4634 prep in,decrease
R2909 T4637 T4638 nummod 12,month
R2910 T4638 T4640 npadvmod month,old
R2911 T4639 T4638 punct -,month
R2912 T4640 T4642 amod old,mice
R2913 T4641 T4640 punct -,old
R2914 T4642 T4636 pobj mice,in
R2915 T4643 T4642 nmod Sam68,mice
R2916 T4644 T4643 punct −,Sam68
R2917 T4645 T4643 punct /,Sam68
R2918 T4646 T4643 punct −,Sam68
R2919 T4647 T4642 cc and,mice
R2920 T4648 T4649 punct (,S3
R2921 T4649 T4642 conj S3,mice
R2922 T4650 T4649 compound Dataset,S3
R2923 T4651 T4607 punct ),showed
R2924 T4652 T4607 punct .,showed
R2925 T4654 T4655 compound Serum,estrogen
R2926 T4655 T4656 nsubjpass estrogen,decreased
R2927 T4657 T4656 auxpass was,decreased
R2928 T4658 T4656 advmod significantly,decreased
R2929 T4659 T4656 prep in,decreased
R2930 T4660 T4661 det all,mice
R2931 T4661 T4659 pobj mice,in
R2932 T4662 T4663 nummod 12,month
R2933 T4663 T4665 npadvmod month,old
R2934 T4664 T4663 punct -,month
R2935 T4665 T4661 amod old,mice
R2936 T4666 T4665 punct -,old
R2937 T4667 T4656 advmod regardless,decreased
R2938 T4668 T4667 prep of,regardless
R2939 T4669 T4668 pobj genotype,of
R2940 T4670 T4656 prep with,decreased
R2941 T4671 T4672 det a,increase
R2942 T4672 T4670 pobj increase,with
R2943 T4673 T4672 amod reciprocal,increase
R2944 T4674 T4672 prep in,increase
R2945 T4675 T4676 nmod interleukin,levels
R2946 T4676 T4674 pobj levels,in
R2947 T4677 T4675 punct -,interleukin
R2948 T4678 T4675 nummod 6,interleukin
R2949 T4679 T4680 punct (,S3
R2950 T4680 T4656 parataxis S3,decreased
R2951 T4681 T4680 compound Dataset,S3
R2952 T4682 T4680 punct ),S3
R2953 T4683 T4656 punct .,decreased
R2954 T4685 T4686 prep Given,measured
R2955 T4687 T4688 det the,involvement
R2956 T4688 T4685 pobj involvement,Given
R2957 T4689 T4688 prep of,involvement
R2958 T4690 T4689 pobj leptin,of
R2959 T4691 T4688 prep in,involvement
R2960 T4692 T4691 pcomp regulating,in
R2961 T4693 T4694 nmod body,mass
R2962 T4694 T4692 dobj mass,regulating
R2963 T4695 T4693 cc and,body
R2964 T4696 T4693 conj bone,body
R2965 T4697 T4686 punct ", ",measured
R2966 T4698 T4699 compound serum,leptin
R2967 T4699 T4686 nsubjpass leptin,measured
R2968 T4700 T4686 auxpass was,measured
R2969 T4701 T4686 prep in,measured
R2970 T4702 T4703 nmod Sam68,mice
R2971 T4703 T4701 pobj mice,in
R2972 T4704 T4702 punct +,Sam68
R2973 T4705 T4702 punct /,Sam68
R2974 T4706 T4702 punct +,Sam68
R2975 T4707 T4702 cc and,Sam68
R2976 T4708 T4702 conj Sam68,Sam68
R2977 T4709 T4708 punct −,Sam68
R2978 T4710 T4708 punct /,Sam68
R2979 T4711 T4708 punct −,Sam68
R2980 T4712 T4686 punct .,measured
R2981 T4714 T4715 nummod Twelve,month
R2982 T4715 T4717 npadvmod month,old
R2983 T4716 T4715 punct -,month
R2984 T4717 T4719 amod old,mice
R2985 T4718 T4717 punct -,old
R2986 T4719 T4724 nsubj mice,had
R2987 T4720 T4719 nmod Sam68,mice
R2988 T4721 T4720 punct −,Sam68
R2989 T4722 T4720 punct /,Sam68
R2990 T4723 T4720 punct −,Sam68
R2991 T4725 T4726 advmod significantly,lower
R2992 T4726 T4727 amod lower,levels
R2993 T4727 T4724 dobj levels,had
R2994 T4728 T4727 prep than,levels
R2995 T4729 T4730 amod young,mice
R2996 T4730 T4728 pobj mice,than
R2997 T4731 T4729 cc and,young
R2998 T4732 T4729 conj old,young
R2999 T4733 T4730 nmod Sam68,mice
R3000 T4734 T4733 punct +,Sam68
R3001 T4735 T4733 punct /,Sam68
R3002 T4736 T4733 punct +,Sam68
R3003 T4737 T4730 cc and,mice
R3004 T4738 T4739 amod young,mice
R3005 T4739 T4730 conj mice,mice
R3006 T4740 T4739 nmod Sam68,mice
R3007 T4741 T4740 punct −,Sam68
R3008 T4742 T4740 punct /,Sam68
R3009 T4743 T4740 punct −,Sam68
R3010 T4744 T4745 punct (,S3
R3011 T4745 T4724 parataxis S3,had
R3012 T4746 T4745 compound Dataset,S3
R3013 T4747 T4745 punct ),S3
R3014 T4748 T4724 punct .,had
R3015 T4750 T4751 advcl Taken,suggested
R3016 T4752 T4750 advmod together,Taken
R3017 T4753 T4751 punct ", ",suggested
R3018 T4754 T4755 det these,observations
R3019 T4755 T4751 nsubj observations,suggested
R3020 T4756 T4757 mark that,was
R3021 T4757 T4751 ccomp was,suggested
R3022 T4758 T4757 nsubj preservation,was
R3023 T4759 T4758 prep of,preservation
R3024 T4760 T4761 compound bone,mass
R3025 T4761 T4759 pobj mass,of
R3026 T4762 T4758 prep in,preservation
R3027 T4763 T4764 det the,mice
R3028 T4764 T4762 pobj mice,in
R3029 T4765 T4764 nmod Sam68,mice
R3030 T4766 T4765 punct −,Sam68
R3031 T4767 T4765 punct /,Sam68
R3032 T4768 T4765 punct −,Sam68
R3033 T4769 T4757 neg not,was
R3034 T4770 T4757 prep due,was
R3035 T4771 T4770 advmod primarily,due
R3036 T4772 T4770 pcomp to,due
R3037 T4773 T4774 amod altered,status
R3038 T4774 T4770 pobj status,due
R3039 T4775 T4774 compound estrogen,status
R3040 T4776 T4757 cc but,was
R3041 T4777 T4778 aux could,influenced
R3042 T4778 T4757 conj influenced,was
R3043 T4779 T4778 aux have,influenced
R3044 T4780 T4778 auxpass been,influenced
R3045 T4781 T4778 agent by,influenced
R3046 T4782 T4781 pobj differences,by
R3047 T4783 T4782 prep in,differences
R3048 T4784 T4785 amod circulating,levels
R3049 T4785 T4783 pobj levels,in
R3050 T4786 T4785 compound leptin,levels
R3051 T4787 T4751 punct .,suggested
R3052 T4956 T4957 nmod Osteoblast,Activity
R3053 T4957 T4963 nsubjpass Activity,Altered
R3054 T4958 T4956 punct ", ",Osteoblast
R3055 T4959 T4956 cc but,Osteoblast
R3056 T4960 T4959 neg Not,but
R3057 T4961 T4956 conj Osteoclast,Osteoblast
R3058 T4962 T4957 punct ", ",Activity
R3059 T4964 T4963 auxpass Is,Altered
R3060 T4965 T4963 prep in,Altered
R3061 T4966 T4967 nmod Sam68,Mice
R3062 T4967 T4965 pobj Mice,in
R3063 T4968 T4966 punct −,Sam68
R3064 T4969 T4966 punct /,Sam68
R3065 T4970 T4966 punct −,Sam68
R3066 T4971 T4972 advmod Ex,Vivo
R3067 T4972 T4963 advmod Vivo,Altered
R3068 T4974 T4975 nsubj Maintenance,is
R3069 T4976 T4974 prep of,Maintenance
R3070 T4977 T4978 compound bone,mass
R3071 T4978 T4976 pobj mass,of
R3072 T4979 T4974 prep during,Maintenance
R3073 T4980 T4981 det the,cycle
R3074 T4981 T4979 pobj cycle,during
R3075 T4982 T4981 amod adult,cycle
R3076 T4983 T4981 compound remodeling,cycle
R3077 T4984 T4975 acomp dependent,is
R3078 T4985 T4984 prep on,dependent
R3079 T4986 T4987 det the,coupling
R3080 T4987 T4985 pobj coupling,on
R3081 T4988 T4987 prep of,coupling
R3082 T4989 T4988 pobj osteoblast,of
R3083 T4990 T4987 prep to,coupling
R3084 T4991 T4990 pobj osteoclast,to
R3085 T4992 T4987 appos activity,coupling
R3086 T4993 T4975 punct ", ",is
R3087 T4994 T4995 amod such,is
R3088 T4995 T4975 advcl is,is
R3089 T4996 T4995 mark that,is
R3090 T4997 T4995 expl there,is
R3091 T4998 T4999 det no,gain
R3092 T4999 T4995 attr gain,is
R3093 T5000 T4999 amod net,gain
R3094 T5001 T4999 cc or,gain
R3095 T5002 T4999 conj loss,gain
R3096 T5003 T4999 prep of,gain
R3097 T5004 T5003 pobj bone,of
R3098 T5005 T5006 punct [,49
R3099 T5006 T4975 parataxis 49,is
R3100 T5007 T5006 punct ],49
R3101 T5008 T4975 punct .,is
R3102 T5010 T5011 aux To,explore
R3103 T5011 T5013 advcl explore,examined
R3104 T5012 T5011 advmod further,explore
R3105 T5014 T5015 det the,advantage
R3106 T5015 T5011 dobj advantage,explore
R3107 T5016 T5017 npadvmod age,related
R3108 T5017 T5015 amod related,advantage
R3109 T5018 T5017 punct -,related
R3110 T5019 T5015 prep of,advantage
R3111 T5020 T5021 nmod Sam68,mice
R3112 T5021 T5019 pobj mice,of
R3113 T5022 T5020 punct −,Sam68
R3114 T5023 T5020 punct /,Sam68
R3115 T5024 T5020 punct −,Sam68
R3116 T5025 T5011 prep with,explore
R3117 T5026 T5025 pobj respect,with
R3118 T5027 T5026 prep to,respect
R3119 T5028 T5029 compound bone,preservation
R3120 T5029 T5027 pobj preservation,to
R3121 T5030 T5013 punct ", ",examined
R3122 T5031 T5013 nsubj we,examined
R3123 T5032 T5033 det the,activity
R3124 T5033 T5013 dobj activity,examined
R3125 T5034 T5033 amod functional,activity
R3126 T5035 T5033 prep of,activity
R3127 T5036 T5035 pobj Sam68,of
R3128 T5037 T5036 punct −,Sam68
R3129 T5038 T5036 punct /,Sam68
R3130 T5039 T5036 punct −,Sam68
R3131 T5040 T5036 appos osteoblasts,Sam68
R3132 T5041 T5040 cc and,osteoblasts
R3133 T5042 T5040 conj osteoclasts,osteoblasts
R3134 T5043 T5044 advmod ex,vivo
R3135 T5044 T5013 advmod vivo,examined
R3136 T5045 T5046 punct (,6A
R3137 T5046 T5013 parataxis 6A,examined
R3138 T5047 T5046 compound Figure,6A
R3139 T5048 T5046 cc and,6A
R3140 T5049 T5046 conj 6B,6A
R3141 T5050 T5046 punct ),6A
R3142 T5051 T5013 punct .,examined
R3143 T5053 T5054 nsubj Cultures,demonstrated
R3144 T5055 T5053 prep of,Cultures
R3145 T5056 T5057 nmod bone,marrow
R3146 T5057 T5058 nmod marrow,cells
R3147 T5058 T5055 pobj cells,of
R3148 T5059 T5058 amod stromal,cells
R3149 T5060 T5053 acl harvested,Cultures
R3150 T5061 T5060 prep from,harvested
R3151 T5062 T5063 nummod 4,week
R3152 T5063 T5065 npadvmod week,old
R3153 T5064 T5063 punct -,week
R3154 T5065 T5067 amod old,mice
R3155 T5066 T5065 punct -,old
R3156 T5067 T5061 pobj mice,from
R3157 T5068 T5067 nmod Sam68,mice
R3158 T5069 T5068 punct −,Sam68
R3159 T5070 T5068 punct /,Sam68
R3160 T5071 T5068 punct −,Sam68
R3161 T5072 T5060 cc and,harvested
R3162 T5073 T5060 conj maintained,harvested
R3163 T5074 T5073 prep for,maintained
R3164 T5075 T5076 nummod 18,days
R3165 T5076 T5074 pobj days,for
R3166 T5077 T5073 prep in,maintained
R3167 T5078 T5079 compound osteoblast,differentiation
R3168 T5079 T5080 compound differentiation,medium
R3169 T5080 T5077 pobj medium,in
R3170 T5081 T5082 advmod more,intense
R3171 T5082 T5083 amod intense,staining
R3172 T5083 T5054 dobj staining,demonstrated
R3173 T5084 T5083 prep for,staining
R3174 T5085 T5084 pobj ALP,for
R3175 T5086 T5054 prep at,demonstrated
R3176 T5087 T5088 nummod 6,days
R3177 T5088 T5086 pobj days,at
R3178 T5089 T5090 punct (,data
R3179 T5090 T5088 meta data,days
R3180 T5091 T5090 amod unpublished,data
R3181 T5092 T5090 punct ),data
R3182 T5093 T5088 cc and,days
R3183 T5094 T5095 nummod 18,days
R3184 T5095 T5088 conj days,days
R3185 T5096 T5097 punct (,6A
R3186 T5097 T5095 parataxis 6A,days
R3187 T5098 T5097 compound Figure,6A
R3188 T5099 T5097 dep ),6A
R3189 T5100 T5054 dep ", ",demonstrated
R3190 T5101 T5054 cc and,demonstrated
R3191 T5102 T5103 amod more,nodules
R3192 T5103 T5054 conj nodules,demonstrated
R3193 T5104 T5103 amod mineralized,nodules
R3194 T5105 T5103 prep at,nodules
R3195 T5106 T5107 nummod 18,days
R3196 T5107 T5105 pobj days,at
R3197 T5108 T5054 dep ", ",demonstrated
R3198 T5109 T5054 prep than,demonstrated
R3199 T5110 T5111 det the,mice
R3200 T5111 T5109 pobj mice,than
R3201 T5112 T5111 nmod Sam68,mice
R3202 T5113 T5112 punct +,Sam68
R3203 T5114 T5112 punct /,Sam68
R3204 T5115 T5112 punct +,Sam68
R3205 T5116 T5054 dep ", ",demonstrated
R3206 T5117 T5118 advmod even,observed
R3207 T5118 T5054 advcl observed,demonstrated
R3208 T5119 T5118 mark though,observed
R3209 T5120 T5121 amod similar,amounts
R3210 T5121 T5118 nsubjpass amounts,observed
R3211 T5122 T5121 prep of,amounts
R3212 T5123 T5124 nmod fibroblast,units
R3213 T5124 T5122 pobj units,of
R3214 T5125 T5126 npadvmod colony,forming
R3215 T5126 T5124 amod forming,units
R3216 T5127 T5126 punct -,forming
R3217 T5128 T5124 punct (,units
R3218 T5129 T5130 compound CFU,F
R3219 T5130 T5124 appos F,units
R3220 T5131 T5130 punct -,F
R3221 T5132 T5118 punct ),observed
R3222 T5133 T5118 auxpass were,observed
R3223 T5134 T5135 punct (,6A
R3224 T5135 T5118 parataxis 6A,observed
R3225 T5136 T5135 compound Figure,6A
R3226 T5137 T5135 cc and,6A
R3227 T5138 T5139 amod unpublished,data
R3228 T5139 T5135 conj data,6A
R3229 T5140 T5135 punct ),6A
R3230 T5141 T5054 punct .,demonstrated
R3231 T5143 T5144 compound RT,PCR
R3232 T5144 T5146 compound PCR,analysis
R3233 T5145 T5144 punct -,PCR
R3234 T5146 T5147 nsubj analysis,revealed
R3235 T5148 T5146 prep of,analysis
R3236 T5149 T5150 amod molecular,markers
R3237 T5150 T5148 pobj markers,of
R3238 T5151 T5150 prep of,markers
R3239 T5152 T5153 compound osteoblast,differentiation
R3240 T5153 T5151 pobj differentiation,of
R3241 T5154 T5155 punct (,S2
R3242 T5155 T5146 parataxis S2,analysis
R3243 T5156 T5155 compound Dataset,S2
R3244 T5157 T5155 punct ),S2
R3245 T5158 T5159 amod similar,increases
R3246 T5159 T5147 dobj increases,revealed
R3247 T5160 T5147 prep over,revealed
R3248 T5161 T5160 pobj time,over
R3249 T5162 T5147 prep in,revealed
R3250 T5163 T5162 pobj RNA,in
R3251 T5164 T5163 prep from,RNA
R3252 T5165 T5166 nmod Sam68,mice
R3253 T5166 T5164 pobj mice,from
R3254 T5167 T5165 punct +,Sam68
R3255 T5168 T5165 punct /,Sam68
R3256 T5169 T5165 punct +,Sam68
R3257 T5170 T5165 cc and,Sam68
R3258 T5171 T5165 conj Sam68,Sam68
R3259 T5172 T5171 punct −,Sam68
R3260 T5173 T5171 punct /,Sam68
R3261 T5174 T5171 punct −,Sam68
R3262 T5175 T5147 punct ", ",revealed
R3263 T5176 T5177 mark although,appeared
R3264 T5177 T5147 advcl appeared,revealed
R3265 T5178 T5179 nmod type,collagen
R3266 T5179 T5177 nsubj collagen,appeared
R3267 T5180 T5178 nummod I,type
R3268 T5181 T5182 aux to,regulated
R3269 T5182 T5177 xcomp regulated,appeared
R3270 T5183 T5182 auxpass be,regulated
R3271 T5184 T5182 advmod up,regulated
R3272 T5185 T5182 punct -,regulated
R3273 T5186 T5182 prep at,regulated
R3274 T5187 T5188 det both,timepoints
R3275 T5188 T5186 pobj timepoints,at
R3276 T5189 T5182 prep in,regulated
R3277 T5190 T5191 det the,mice
R3278 T5191 T5189 pobj mice,in
R3279 T5192 T5191 nmod Sam68,mice
R3280 T5193 T5192 punct −,Sam68
R3281 T5194 T5192 punct /,Sam68
R3282 T5195 T5192 punct −,Sam68
R3283 T5196 T5147 punct .,revealed
R3284 T5198 T5199 amod Short,term
R3285 T5199 T5201 compound term,cultures
R3286 T5200 T5199 punct -,term
R3287 T5201 T5202 nsubj cultures,stained
R3288 T5203 T5201 prep of,cultures
R3289 T5204 T5205 amod mature,osteoclasts
R3290 T5205 T5203 pobj osteoclasts,of
R3291 T5206 T5205 acl released,osteoclasts
R3292 T5207 T5206 prep from,released
R3293 T5208 T5209 det the,bones
R3294 T5209 T5207 pobj bones,from
R3295 T5210 T5209 amod crushed,bones
R3296 T5211 T5209 amod long,bones
R3297 T5212 T5209 prep of,bones
R3298 T5213 T5214 nmod Sam68,mice
R3299 T5214 T5212 pobj mice,of
R3300 T5215 T5213 punct +,Sam68
R3301 T5216 T5213 punct /,Sam68
R3302 T5217 T5213 punct +,Sam68
R3303 T5218 T5213 cc and,Sam68
R3304 T5219 T5213 conj Sam68,Sam68
R3305 T5220 T5219 punct −,Sam68
R3306 T5221 T5219 punct /,Sam68
R3307 T5222 T5219 punct −,Sam68
R3308 T5223 T5224 advmod equally,well
R3309 T5224 T5202 advmod well,stained
R3310 T5225 T5202 prep for,stained
R3311 T5226 T5225 pobj TRAP,for
R3312 T5227 T5202 cc and,stained
R3313 T5228 T5202 conj excavated,stained
R3314 T5229 T5230 advmod approximately,equal
R3315 T5230 T5231 amod equal,numbers
R3316 T5231 T5228 dobj numbers,excavated
R3317 T5232 T5231 prep of,numbers
R3318 T5233 T5232 pobj pits,of
R3319 T5234 T5233 prep of,pits
R3320 T5235 T5236 amod equal,size
R3321 T5236 T5234 pobj size,of
R3322 T5237 T5228 prep in,excavated
R3323 T5238 T5239 compound dentin,slices
R3324 T5239 T5237 pobj slices,in
R3325 T5240 T5228 punct ", ",excavated
R3326 T5241 T5242 mark as,visualized
R3327 T5242 T5228 advcl visualized,excavated
R3328 T5243 T5242 prep by,visualized
R3329 T5244 T5243 pcomp scanning,by
R3330 T5245 T5246 compound electron,microscopy
R3331 T5246 T5244 dobj microscopy,scanning
R3332 T5247 T5248 punct (,6B
R3333 T5248 T5228 parataxis 6B,excavated
R3334 T5249 T5248 compound Figure,6B
R3335 T5250 T5248 punct ),6B
R3336 T5251 T5202 punct .,stained
R3337 T5253 T5254 det These,findings
R3338 T5254 T5255 nsubj findings,suggest
R3339 T5256 T5257 mark that,be
R3340 T5257 T5255 ccomp be,suggest
R3341 T5258 T5259 det the,osteoclasts
R3342 T5259 T5257 nsubj osteoclasts,be
R3343 T5260 T5257 aux may,be
R3344 T5261 T5257 neg not,be
R3345 T5262 T5263 det the,defect
R3346 T5263 T5257 attr defect,be
R3347 T5264 T5263 amod primary,defect
R3348 T5265 T5263 prep in,defect
R3349 T5266 T5267 nmod Sam68,mice
R3350 T5267 T5265 pobj mice,in
R3351 T5268 T5266 punct −,Sam68
R3352 T5269 T5266 punct /,Sam68
R3353 T5270 T5266 punct −,Sam68
R3354 T5271 T5257 punct ", ",be
R3355 T5272 T5273 mark as,are
R3356 T5273 T5257 advcl are,be
R3357 T5274 T5273 nsubj they,are
R3358 T5275 T5273 prep in,are
R3359 T5276 T5277 nmod Src,mice
R3360 T5277 T5275 pobj mice,in
R3361 T5278 T5277 amod null,mice
R3362 T5279 T5280 punct [,48
R3363 T5280 T5273 parataxis 48,are
R3364 T5281 T5280 punct ],48
R3365 T5282 T5255 punct .,suggest
R3377 T5689 T5690 det The,Absence
R3378 T5690 T5691 nsubj Absence,Prevents
R3379 T5692 T5690 prep of,Absence
R3380 T5693 T5692 pobj Sam68,of
R3381 T5694 T5695 compound Adipocyte,Differentiation
R3382 T5695 T5691 dobj Differentiation,Prevents
R3383 T5696 T5691 cc and,Prevents
R3384 T5697 T5691 conj Promotes,Prevents
R3385 T5698 T5699 compound Osteoblast,Differentiation
R3386 T5699 T5697 dobj Differentiation,Promotes
R3387 T5701 T5702 nsubjpass It,recognized
R3388 T5703 T5702 auxpass is,recognized
R3389 T5704 T5702 advmod well,recognized
R3390 T5705 T5706 mark that,give
R3391 T5706 T5702 advcl give,recognized
R3392 T5707 T5708 nmod bone,marrow
R3393 T5708 T5709 nmod marrow,cells
R3394 T5709 T5706 nsubj cells,give
R3395 T5710 T5709 amod stromal,cells
R3396 T5711 T5706 dobj rise,give
R3397 T5712 T5706 prep to,give
R3398 T5713 T5714 det both,osteoblasts
R3399 T5714 T5712 pobj osteoblasts,to
R3400 T5715 T5714 cc and,osteoblasts
R3401 T5716 T5714 conj adipocytes,osteoblasts
R3402 T5717 T5706 cc and,give
R3403 T5718 T5719 mark that,accompanied
R3404 T5719 T5706 conj accompanied,give
R3405 T5720 T5721 npadvmod age,related
R3406 T5721 T5723 amod related,loss
R3407 T5722 T5721 punct -,related
R3408 T5723 T5719 nsubjpass loss,accompanied
R3409 T5724 T5723 compound bone,loss
R3410 T5725 T5719 auxpass is,accompanied
R3411 T5726 T5719 agent by,accompanied
R3412 T5727 T5728 det an,increase
R3413 T5728 T5726 pobj increase,by
R3414 T5729 T5728 prep in,increase
R3415 T5730 T5729 pobj differentiation,in
R3416 T5731 T5719 prep down,accompanied
R3417 T5732 T5733 det the,lineage
R3418 T5733 T5731 pobj lineage,down
R3419 T5734 T5733 compound adipocyte,lineage
R3420 T5735 T5736 punct [,22
R3421 T5736 T5719 parataxis 22,accompanied
R3422 T5737 T5736 punct ],22
R3423 T5738 T5702 punct .,recognized
R3424 T5740 T5741 advmod Therefore,influence
R3425 T5742 T5741 punct ", ",influence
R3426 T5743 T5744 det the,loss
R3427 T5744 T5741 nsubj loss,influence
R3428 T5745 T5744 prep of,loss
R3429 T5746 T5745 pobj Sam68,of
R3430 T5747 T5741 aux could,influence
R3431 T5748 T5749 det the,cells
R3432 T5749 T5741 dobj cells,influence
R3433 T5750 T5751 nmod bone,marrow
R3434 T5751 T5749 nmod marrow,cells
R3435 T5752 T5749 amod stromal,cells
R3436 T5753 T5754 aux to,differentiate
R3437 T5754 T5741 advcl differentiate,influence
R3438 T5755 T5754 prep along,differentiate
R3439 T5756 T5757 det the,osteogenic
R3440 T5757 T5755 pobj osteogenic,along
R3441 T5758 T5757 cc versus,osteogenic
R3442 T5759 T5760 det the,adipogenic
R3443 T5760 T5761 nmod adipogenic,pathway
R3444 T5761 T5757 conj pathway,osteogenic
R3445 T5762 T5741 punct .,influence
R3446 T5764 T5765 advmod Alternatively,regulate
R3447 T5766 T5765 punct ", ",regulate
R3448 T5767 T5768 det the,loss
R3449 T5768 T5765 nsubj loss,regulate
R3450 T5769 T5768 prep of,loss
R3451 T5770 T5769 pobj Sam68,of
R3452 T5771 T5765 aux could,regulate
R3453 T5772 T5765 advmod indirectly,regulate
R3454 T5773 T5774 compound bone,mass
R3455 T5774 T5765 dobj mass,regulate
R3456 T5775 T5765 prep as,regulate
R3457 T5776 T5777 det a,disturbance
R3458 T5777 T5775 pobj disturbance,as
R3459 T5778 T5777 amod general,disturbance
R3460 T5779 T5777 prep of,disturbance
R3461 T5780 T5781 amod neuroendocrine,control
R3462 T5781 T5779 pobj control,of
R3463 T5782 T5783 mark as,shown
R3464 T5783 T5765 advcl shown,regulate
R3465 T5784 T5783 auxpass was,shown
R3466 T5785 T5786 advmod when,shown
R3467 T5786 T5783 advcl shown,shown
R3468 T5787 T5786 nsubjpass leptin,shown
R3469 T5788 T5787 cc and,leptin
R3470 T5789 T5790 det the,axis
R3471 T5790 T5787 conj axis,leptin
R3472 T5791 T5790 amod sympathetic,axis
R3473 T5792 T5793 amod nervous,system
R3474 T5793 T5790 compound system,axis
R3475 T5794 T5786 auxpass were,shown
R3476 T5795 T5796 aux to,regulate
R3477 T5796 T5786 xcomp regulate,shown
R3478 T5797 T5796 advmod negatively,regulate
R3479 T5798 T5799 compound bone,mass
R3480 T5799 T5796 dobj mass,regulate
R3481 T5800 T5801 punct [,8
R3482 T5801 T5765 parataxis 8,regulate
R3483 T5802 T5801 punct ],8
R3484 T5803 T5765 punct .,regulate
R3485 T5805 T5806 aux To,confirm
R3486 T5806 T5807 advcl confirm,isolated
R3487 T5808 T5809 det a,role
R3488 T5809 T5806 dobj role,confirm
R3489 T5810 T5809 prep for,role
R3490 T5811 T5810 pobj Sam68,for
R3491 T5812 T5809 prep in,role
R3492 T5813 T5814 det the,regulation
R3493 T5814 T5812 pobj regulation,in
R3494 T5815 T5814 prep of,regulation
R3495 T5816 T5817 compound adipocyte,differentiation
R3496 T5817 T5815 pobj differentiation,of
R3497 T5818 T5807 punct ", ",isolated
R3498 T5819 T5807 nsubj we,isolated
R3499 T5820 T5821 amod primary,MEFs
R3500 T5821 T5807 dobj MEFs,isolated
R3501 T5822 T5821 prep from,MEFs
R3502 T5823 T5824 nummod 14.5,day
R3503 T5824 T5826 npadvmod day,old
R3504 T5825 T5824 punct -,day
R3505 T5826 T5828 amod old,embryos
R3506 T5827 T5826 punct -,old
R3507 T5828 T5822 pobj embryos,from
R3508 T5829 T5828 nmod Sam68,embryos
R3509 T5830 T5829 punct +,Sam68
R3510 T5831 T5829 punct /,Sam68
R3511 T5832 T5829 punct +,Sam68
R3512 T5833 T5829 cc and,Sam68
R3513 T5834 T5829 conj Sam68,Sam68
R3514 T5835 T5834 punct −,Sam68
R3515 T5836 T5834 punct /,Sam68
R3516 T5837 T5834 punct −,Sam68
R3517 T5838 T5807 punct .,isolated
R3518 T5840 T5841 det The,MEFs
R3519 T5841 T5847 nsubjpass MEFs,differentiated
R3520 T5842 T5841 amod primary,MEFs
R3521 T5843 T5841 nmod Sam68,MEFs
R3522 T5844 T5843 punct −,Sam68
R3523 T5845 T5843 punct /,Sam68
R3524 T5846 T5843 punct −,Sam68
R3525 T5848 T5847 auxpass were,differentiated
R3526 T5849 T5847 prep into,differentiated
R3527 T5850 T5849 pobj adipocytes,into
R3528 T5851 T5852 advmod in,vitro
R3529 T5852 T5847 advmod vitro,differentiated
R3530 T5853 T5847 prep in,differentiated
R3531 T5854 T5855 compound culture,medium
R3532 T5855 T5853 pobj medium,in
R3533 T5856 T5855 acl containing,medium
R3534 T5857 T5858 nummod 5,μM
R3535 T5858 T5859 compound μM,pioglitazone
R3536 T5859 T5856 dobj pioglitazone,containing
R3537 T5860 T5861 aux to,induce
R3538 T5861 T5847 advcl induce,differentiated
R3539 T5862 T5861 dobj adipogenesis,induce
R3540 T5863 T5847 punct .,differentiated
R3541 T5865 T5866 nsubjpass Cells,stained
R3542 T5867 T5865 prep at,Cells
R3543 T5868 T5869 nmod days,0
R3544 T5869 T5867 pobj 0,at
R3545 T5870 T5869 punct ", ",0
R3546 T5871 T5869 conj 4,0
R3547 T5872 T5871 punct ", ",4
R3548 T5873 T5871 conj 6,4
R3549 T5874 T5873 punct ", ",6
R3550 T5875 T5873 cc and,6
R3551 T5876 T5873 conj 12,6
R3552 T5877 T5866 auxpass were,stained
R3553 T5878 T5866 prep with,stained
R3554 T5879 T5880 compound Oil,O
R3555 T5880 T5878 pobj O,with
R3556 T5881 T5880 compound red,O
R3557 T5882 T5883 aux to,monitor
R3558 T5883 T5866 advcl monitor,stained
R3559 T5884 T5883 dobj adipogenesis,monitor
R3560 T5885 T5866 punct .,stained
R3561 T5887 T5888 nsubj Adipogenesis,was
R3562 T5889 T5890 advmod more,pronounced
R3563 T5890 T5888 acomp pronounced,was
R3564 T5891 T5888 prep in,was
R3565 T5892 T5893 det the,cultures
R3566 T5893 T5891 pobj cultures,in
R3567 T5894 T5895 amod wild,type
R3568 T5895 T5893 compound type,cultures
R3569 T5896 T5895 punct -,type
R3570 T5897 T5893 compound MEFs,cultures
R3571 T5898 T5888 prep than,was
R3572 T5899 T5898 prep in,than
R3573 T5900 T5901 det the,MEFs
R3574 T5901 T5899 pobj MEFs,in
R3575 T5902 T5901 nmod Sam68,MEFs
R3576 T5903 T5902 punct −,Sam68
R3577 T5904 T5902 punct /,Sam68
R3578 T5905 T5902 punct −,Sam68
R3579 T5906 T5888 punct ", ",was
R3580 T5907 T5888 advcl consistent,was
R3581 T5908 T5907 prep with,consistent
R3582 T5909 T5910 det the,role
R3583 T5910 T5908 pobj role,with
R3584 T5911 T5910 amod positive,role
R3585 T5912 T5910 prep of,role
R3586 T5913 T5914 amod endogenous,Sam68
R3587 T5914 T5912 pobj Sam68,of
R3588 T5915 T5910 prep in,role
R3589 T5916 T5917 compound adipocyte,differentiation
R3590 T5917 T5915 pobj differentiation,in
R3591 T5918 T5919 punct (,Figure
R3592 T5919 T5888 parataxis Figure,was
R3593 T5920 T5919 nummod 7,Figure
R3594 T5921 T5919 punct ),Figure
R3595 T5922 T5888 punct .,was
R3596 T5924 T5925 det The,expression
R3597 T5925 T5926 nsubjpass expression,impaired
R3598 T5927 T5925 prep of,expression
R3599 T5928 T5929 amod key,factors
R3600 T5929 T5927 pobj factors,of
R3601 T5930 T5929 compound transcription,factors
R3602 T5931 T5929 prep including,factors
R3603 T5932 T5933 det the,PPARγ
R3604 T5933 T5931 pobj PPARγ,including
R3605 T5934 T5933 cc and,PPARγ
R3606 T5935 T5933 conj KLF5,PPARγ
R3607 T5936 T5926 auxpass was,impaired
R3608 T5937 T5926 prep in,impaired
R3609 T5938 T5939 nmod Sam68,MEFs
R3610 T5939 T5937 pobj MEFs,in
R3611 T5940 T5938 punct −,Sam68
R3612 T5941 T5938 punct /,Sam68
R3613 T5942 T5938 punct −,Sam68
R3614 T5943 T5939 amod differentiated,MEFs
R3615 T5944 T5926 prep compared,impaired
R3616 T5945 T5944 prep with,compared
R3617 T5946 T5947 nmod Sam68,MEFs
R3618 T5947 T5945 pobj MEFs,with
R3619 T5948 T5946 punct +,Sam68
R3620 T5949 T5946 punct /,Sam68
R3621 T5950 T5946 punct +,Sam68
R3622 T5951 T5926 punct ", ",impaired
R3623 T5952 T5926 advcl consistent,impaired
R3624 T5953 T5952 prep with,consistent
R3625 T5954 T5955 amod impaired,adipogenesis
R3626 T5955 T5953 pobj adipogenesis,with
R3627 T5956 T5955 prep in,adipogenesis
R3628 T5957 T5958 det the,absence
R3629 T5958 T5956 pobj absence,in
R3630 T5959 T5958 prep of,absence
R3631 T5960 T5959 pobj Sam68,of
R3632 T5961 T5962 punct (,Figure
R3633 T5962 T5926 parataxis Figure,impaired
R3634 T5963 T5962 nummod 7,Figure
R3635 T5964 T5962 punct ),Figure
R3636 T5965 T5926 punct .,impaired
R3637 T5967 T5968 det These,data
R3638 T5968 T5969 nsubj data,support
R3639 T5970 T5968 punct ", ",data
R3640 T5971 T5968 advmod together,data
R3641 T5972 T5971 prep with,together
R3642 T5973 T5972 pobj data,with
R3643 T5974 T5973 acl confirming,data
R3644 T5975 T5976 det a,phenotype
R3645 T5976 T5974 dobj phenotype,confirming
R3646 T5977 T5976 amod lean,phenotype
R3647 T5978 T5976 prep in,phenotype
R3648 T5979 T5980 nmod Sam68,mice
R3649 T5980 T5978 pobj mice,in
R3650 T5981 T5979 punct −,Sam68
R3651 T5982 T5979 punct /,Sam68
R3652 T5983 T5979 punct −,Sam68
R3653 T5984 T5985 punct (,N.
R3654 T5985 T5968 meta N.,data
R3655 T5986 T5985 nmod Torabi,N.
R3656 T5987 T5985 cc and,N.
R3657 T5988 T5985 conj S.,N.
R3658 T5989 T5988 nmod Richard,S.
R3659 T5990 T5988 punct ", ",S.
R3660 T5991 T5992 amod unpublished,data
R3661 T5992 T5988 conj data,S.
R3662 T5993 T5992 punct ),data
R3663 T5994 T5969 punct ", ",support
R3664 T5995 T5996 det the,hypothesis
R3665 T5996 T5969 dobj hypothesis,support
R3666 T5997 T5998 mark that,modulates
R3667 T5998 T5996 acl modulates,hypothesis
R3668 T5999 T5998 nsubj Sam68,modulates
R3669 T6000 T6001 det the,differentiation
R3670 T6001 T5998 dobj differentiation,modulates
R3671 T6002 T6001 prep of,differentiation
R3672 T6003 T6004 amod mesenchymal,cells
R3673 T6004 T6002 pobj cells,of
R3674 T6005 T5969 punct .,support
R3675 T6007 T6008 aux To,examine
R3676 T6008 T6010 advcl examine,chose
R3677 T6009 T6008 advmod further,examine
R3678 T6011 T6012 det this,phenotype
R3679 T6012 T6008 dobj phenotype,examine
R3680 T6013 T6008 prep in,examine
R3681 T6014 T6015 det a,system
R3682 T6015 T6013 pobj system,in
R3683 T6016 T6017 npadvmod cell,autonomous
R3684 T6017 T6015 amod autonomous,system
R3685 T6018 T6010 punct ", ",chose
R3686 T6019 T6010 nsubj we,chose
R3687 T6020 T6021 det the,cells
R3688 T6021 T6010 dobj cells,chose
R3689 T6022 T6021 amod embryonic,cells
R3690 T6023 T6021 amod mesenchymal,cells
R3691 T6024 T6021 amod multipotential,cells
R3692 T6025 T6021 compound progenitor,cells
R3693 T6026 T6021 appos C3H10T1,cells
R3694 T6027 T6026 punct /,C3H10T1
R3695 T6028 T6026 nummod 2,C3H10T1
R3696 T6029 T6010 punct .,chose
R3697 T6031 T6032 det The,addition
R3698 T6032 T6033 nsubj addition,induced
R3699 T6034 T6032 prep of,addition
R3700 T6035 T6034 pobj BMP,of
R3701 T6036 T6035 punct -,BMP
R3702 T6037 T6035 nummod 2,BMP
R3703 T6038 T6039 compound osteoblast,differentiation
R3704 T6039 T6033 dobj differentiation,induced
R3705 T6040 T6033 punct ", ",induced
R3706 T6041 T6042 mark as,evidenced
R3707 T6042 T6033 advcl evidenced,induced
R3708 T6043 T6042 agent by,evidenced
R3709 T6044 T6045 det an,increase
R3710 T6045 T6043 pobj increase,by
R3711 T6046 T6047 advmod approximately,400-fold
R3712 T6047 T6045 nmod 400-fold,increase
R3713 T6048 T6045 prep in,increase
R3714 T6049 T6048 pobj expression,in
R3715 T6050 T6049 prep of,expression
R3716 T6051 T6052 det the,gene
R3717 T6052 T6050 pobj gene,of
R3718 T6053 T6052 nmod osteocalcin,gene
R3719 T6054 T6053 punct (,osteocalcin
R3720 T6055 T6053 appos OCN,osteocalcin
R3721 T6056 T6052 punct ),gene
R3722 T6057 T6058 punct [,51
R3723 T6058 T6033 parataxis 51,induced
R3724 T6059 T6058 punct ],51
R3725 T6060 T6033 punct .,induced
R3726 T6062 T6063 nsubjpass Populations,selected
R3727 T6064 T6062 prep of,Populations
R3728 T6065 T6066 nmod C3H10T1,cells
R3729 T6066 T6064 pobj cells,of
R3730 T6067 T6065 punct /,C3H10T1
R3731 T6068 T6065 nummod 2,C3H10T1
R3732 T6069 T6070 advmod stably,transfected
R3733 T6070 T6062 acl transfected,Populations
R3734 T6071 T6070 prep with,transfected
R3735 T6072 T6073 preconj either,retro
R3736 T6073 T6071 pobj retro,with
R3737 T6074 T6073 compound pSuper,retro
R3738 T6075 T6073 punct -,retro
R3739 T6076 T6077 punct (,control
R3740 T6077 T6073 parataxis control,retro
R3741 T6078 T6077 punct ),control
R3742 T6079 T6073 cc and,retro
R3743 T6080 T6081 compound pSuper,retro
R3744 T6081 T6073 conj retro,retro
R3745 T6082 T6081 punct -,retro
R3746 T6083 T6081 acl harboring,retro
R3747 T6084 T6085 det a,hairpin
R3748 T6085 T6083 dobj hairpin,harboring
R3749 T6086 T6085 amod short,hairpin
R3750 T6087 T6085 prep against,hairpin
R3751 T6088 T6087 pobj Sam68,against
R3752 T6089 T6085 punct (,hairpin
R3753 T6090 T6085 appos Sam68sh,hairpin
R3754 T6091 T6063 punct ),selected
R3755 T6092 T6063 auxpass were,selected
R3756 T6093 T6063 prep with,selected
R3757 T6094 T6093 pobj puromycin,with
R3758 T6095 T6063 punct .,selected
R3759 T6097 T6098 det The,expression
R3760 T6098 T6099 nsubjpass expression,reduced
R3761 T6100 T6098 prep of,expression
R3762 T6101 T6100 pobj Sam68,of
R3763 T6102 T6099 auxpass was,reduced
R3764 T6103 T6099 prep by,reduced
R3765 T6104 T6105 advmod approximately,80
R3766 T6105 T6106 nummod 80,%
R3767 T6106 T6103 pobj %,by
R3768 T6107 T6108 mark as,evidenced
R3769 T6108 T6099 advcl evidenced,reduced
R3770 T6109 T6108 agent by,evidenced
R3771 T6110 T6111 compound immunoblot,analyses
R3772 T6111 T6109 pobj analyses,by
R3773 T6112 T6111 acl using,analyses
R3774 T6113 T6114 compound β,actin
R3775 T6114 T6112 dobj actin,using
R3776 T6115 T6114 punct -,actin
R3777 T6116 T6112 prep as,using
R3778 T6117 T6118 det a,control
R3779 T6118 T6116 pobj control,as
R3780 T6119 T6118 compound loading,control
R3781 T6120 T6121 punct (,8A
R3782 T6121 T6099 parataxis 8A,reduced
R3783 T6122 T6121 compound Figure,8A
R3784 T6123 T6121 punct ),8A
R3785 T6124 T6099 punct .,reduced
R3786 T6126 T6127 compound Osteoblast,differentiation
R3787 T6127 T6128 nsubjpass differentiation,induced
R3788 T6129 T6128 auxpass was,induced
R3789 T6130 T6128 prep in,induced
R3790 T6131 T6130 pobj cultures,in
R3791 T6132 T6131 acl expressing,cultures
R3792 T6133 T6134 compound pSuper,retro
R3793 T6134 T6132 dobj retro,expressing
R3794 T6135 T6134 punct -,retro
R3795 T6136 T6134 cc or,retro
R3796 T6137 T6138 compound pSuper,retro
R3797 T6138 T6140 compound retro,Sam68sh
R3798 T6139 T6138 punct -,retro
R3799 T6140 T6134 conj Sam68sh,retro
R3800 T6141 T6128 prep by,induced
R3801 T6142 T6141 pobj addition,by
R3802 T6143 T6142 prep of,addition
R3803 T6144 T6143 pobj BMP,of
R3804 T6145 T6144 punct -,BMP
R3805 T6146 T6144 nummod 2,BMP
R3806 T6147 T6142 prep to,addition
R3807 T6148 T6149 det the,medium
R3808 T6149 T6147 pobj medium,to
R3809 T6150 T6149 compound culture,medium
R3810 T6151 T6128 punct .,induced
R3811 T6152 T6128 punct .,induced
R3812 T6154 T6155 det The,expression
R3813 T6155 T6156 nsubjpass expression,examined
R3814 T6157 T6155 prep of,expression
R3815 T6158 T6157 pobj OCN,of
R3816 T6159 T6158 cc and,OCN
R3817 T6160 T6161 compound β,actin
R3818 T6161 T6163 compound actin,mRNAs
R3819 T6162 T6161 punct -,actin
R3820 T6163 T6158 conj mRNAs,OCN
R3821 T6164 T6156 auxpass was,examined
R3822 T6165 T6156 prep by,examined
R3823 T6166 T6167 amod semiquantitative,PCR
R3824 T6167 T6165 pobj PCR,by
R3825 T6168 T6167 compound RT,PCR
R3826 T6169 T6167 punct -,PCR
R3827 T6170 T6156 cc and,examined
R3828 T6171 T6172 det the,cells
R3829 T6172 T6176 nsubj cells,displayed
R3830 T6173 T6174 npadvmod Sam68sh,expressing
R3831 T6174 T6172 amod expressing,cells
R3832 T6175 T6174 punct -,expressing
R3833 T6176 T6156 conj displayed,examined
R3834 T6177 T6178 det a,phenotype
R3835 T6178 T6176 dobj phenotype,displayed
R3836 T6179 T6180 advmod more,pronounced
R3837 T6180 T6178 amod pronounced,phenotype
R3838 T6181 T6178 compound osteoblast,phenotype
R3839 T6182 T6178 prep compared,phenotype
R3840 T6183 T6182 prep with,compared
R3841 T6184 T6185 compound control,cells
R3842 T6185 T6183 pobj cells,with
R3843 T6186 T6176 punct ", ",displayed
R3844 T6187 T6188 mark as,assessed
R3845 T6188 T6176 advcl assessed,displayed
R3846 T6189 T6188 prep by,assessed
R3847 T6190 T6191 det the,expression
R3848 T6191 T6189 pobj expression,by
R3849 T6192 T6191 prep of,expression
R3850 T6193 T6192 pobj OCN,of
R3851 T6194 T6195 punct (,8B
R3852 T6195 T6176 parataxis 8B,displayed
R3853 T6196 T6195 compound Figure,8B
R3854 T6197 T6195 punct ),8B
R3855 T6198 T6176 punct .,displayed
R3856 T6200 T6201 prep Given,stained
R3857 T6202 T6203 det the,enhancement
R3858 T6203 T6200 pobj enhancement,Given
R3859 T6204 T6203 amod apparent,enhancement
R3860 T6205 T6203 prep of,enhancement
R3861 T6206 T6207 amod mineralized,nodule
R3862 T6207 T6208 compound nodule,formation
R3863 T6208 T6205 pobj formation,of
R3864 T6209 T6203 prep by,enhancement
R3865 T6210 T6211 nmod Sam68,cells
R3866 T6211 T6209 pobj cells,by
R3867 T6212 T6210 punct −,Sam68
R3868 T6213 T6210 punct /,Sam68
R3869 T6214 T6210 punct −,Sam68
R3870 T6215 T6216 nmod bone,marrow
R3871 T6216 T6211 nmod marrow,cells
R3872 T6217 T6211 amod stromal,cells
R3873 T6218 T6219 advmod ex,vivo
R3874 T6219 T6203 advmod vivo,enhancement
R3875 T6220 T6203 cc and,enhancement
R3876 T6221 T6222 det the,phenotype
R3877 T6222 T6203 conj phenotype,enhancement
R3878 T6223 T6222 acl observed,phenotype
R3879 T6224 T6223 prep with,observed
R3880 T6225 T6226 amod short,RNA
R3881 T6226 T6228 npadvmod RNA,treated
R3882 T6227 T6226 compound hairpin,RNA
R3883 T6228 T6233 amod treated,C3H10T1
R3884 T6229 T6226 punct (,RNA
R3885 T6230 T6226 appos shRNA,RNA
R3886 T6231 T6228 punct ),treated
R3887 T6232 T6228 punct -,treated
R3888 T6233 T6224 pobj C3H10T1,with
R3889 T6234 T6233 punct /,C3H10T1
R3890 T6235 T6233 nummod 2,C3H10T1
R3891 T6236 T6201 punct ", ",stained
R3892 T6237 T6201 nsubj we,stained
R3893 T6238 T6201 dobj sections,stained
R3894 T6239 T6238 prep of,sections
R3895 T6240 T6239 pobj bone,of
R3896 T6241 T6238 prep from,sections
R3897 T6242 T6243 nummod 4,month
R3898 T6243 T6248 npadvmod month,old
R3899 T6244 T6242 punct -,4
R3900 T6245 T6242 cc and,4
R3901 T6246 T6242 conj 12,4
R3902 T6247 T6243 punct -,month
R3903 T6248 T6250 amod old,mice
R3904 T6249 T6248 punct -,old
R3905 T6250 T6241 pobj mice,from
R3906 T6251 T6201 prep for,stained
R3907 T6252 T6251 pobj evidence,for
R3908 T6253 T6252 prep of,evidence
R3909 T6254 T6253 pobj changes,of
R3910 T6255 T6254 prep in,changes
R3911 T6256 T6257 compound marrow,adiposity
R3912 T6257 T6255 pobj adiposity,in
R3913 T6258 T6201 punct .,stained
R3914 T6260 T6261 nsubj Figure,shows
R3915 T6262 T6260 nummod 9,Figure
R3916 T6263 T6261 dobj sections,shows
R3917 T6264 T6263 prep of,sections
R3918 T6265 T6266 amod undecalcified,bone
R3919 T6266 T6264 pobj bone,of
R3920 T6267 T6266 prep from,bone
R3921 T6268 T6269 nummod 4,month
R3922 T6269 T6274 npadvmod month,old
R3923 T6270 T6268 punct -,4
R3924 T6271 T6268 cc and,4
R3925 T6272 T6268 conj 12,4
R3926 T6273 T6269 punct -,month
R3927 T6274 T6276 amod old,mice
R3928 T6275 T6274 punct -,old
R3929 T6276 T6267 pobj mice,from
R3930 T6277 T6276 nmod Sam68,mice
R3931 T6278 T6277 punct +,Sam68
R3932 T6279 T6277 punct /,Sam68
R3933 T6280 T6277 punct +,Sam68
R3934 T6281 T6277 cc and,Sam68
R3935 T6282 T6277 conj Sam68,Sam68
R3936 T6283 T6282 punct −,Sam68
R3937 T6284 T6282 punct /,Sam68
R3938 T6285 T6282 punct −,Sam68
R3939 T6286 T6276 acl stained,mice
R3940 T6287 T6286 prep with,stained
R3941 T6288 T6289 compound von,Kossa
R3942 T6289 T6287 pobj Kossa,with
R3943 T6290 T6289 cc and,Kossa
R3944 T6291 T6289 conj toluidine,Kossa
R3945 T6292 T6291 amod blue,toluidine
R3946 T6293 T6294 aux to,show
R3947 T6294 T6286 advcl show,stained
R3948 T6295 T6296 amod mineralized,tissue
R3949 T6296 T6294 dobj tissue,show
R3950 T6297 T6298 punct (,9A
R3951 T6298 T6296 parataxis 9A,tissue
R3952 T6299 T6298 compound Figure,9A
R3953 T6300 T6298 punct ", ",9A
R3954 T6301 T6298 amod black,9A
R3955 T6302 T6298 punct ),9A
R3956 T6303 T6296 cc and,tissue
R3957 T6304 T6305 compound marrow,adipocytes
R3958 T6305 T6296 conj adipocytes,tissue
R3959 T6306 T6307 punct (,9B
R3960 T6307 T6305 parataxis 9B,adipocytes
R3961 T6308 T6307 compound Figure,9B
R3962 T6309 T6307 punct ", ",9B
R3963 T6310 T6307 amod white,9B
R3964 T6311 T6307 punct ),9B
R3965 T6312 T6286 cc and,stained
R3966 T6313 T6286 conj left,stained
R3967 T6314 T6313 oprd unstained,left
R3968 T6315 T6316 aux to,show
R3969 T6316 T6313 advcl show,left
R3970 T6317 T6318 compound fluorochrome,labeling
R3971 T6318 T6316 dobj labeling,show
R3972 T6319 T6318 prep of,labeling
R3973 T6320 T6321 det the,fronts
R3974 T6321 T6319 pobj fronts,of
R3975 T6322 T6321 compound mineralization,fronts
R3976 T6323 T6324 punct (,9C
R3977 T6324 T6316 parataxis 9C,show
R3978 T6325 T6324 compound Figure,9C
R3979 T6326 T6324 punct ),9C
R3980 T6327 T6261 punct .,shows
R3981 T6329 T6330 nummod Twelve,month
R3982 T6330 T6332 npadvmod month,old
R3983 T6331 T6330 punct -,month
R3984 T6332 T6334 amod old,mice
R3985 T6333 T6332 punct -,old
R3986 T6334 T6339 nsubj mice,showed
R3987 T6335 T6334 nmod Sam68,mice
R3988 T6336 T6335 punct +,Sam68
R3989 T6337 T6335 punct /,Sam68
R3990 T6338 T6335 punct +,Sam68
R3991 T6340 T6341 det a,decrease
R3992 T6341 T6339 dobj decrease,showed
R3993 T6342 T6341 amod noticeable,decrease
R3994 T6343 T6341 prep in,decrease
R3995 T6344 T6345 amod trabecular,bone
R3996 T6345 T6343 pobj bone,in
R3997 T6346 T6347 punct (,9A
R3998 T6347 T6345 parataxis 9A,bone
R3999 T6348 T6347 compound Figure,9A
R4000 T6349 T6347 punct ),9A
R4001 T6350 T6341 punct ", ",decrease
R4002 T6351 T6352 dep which,associated
R4003 T6352 T6341 relcl associated,decrease
R4004 T6353 T6352 auxpass was,associated
R4005 T6354 T6352 prep with,associated
R4006 T6355 T6356 det a,increase
R4007 T6356 T6354 pobj increase,with
R4008 T6357 T6356 amod significant,increase
R4009 T6358 T6356 prep in,increase
R4010 T6359 T6360 compound marrow,adiposity
R4011 T6360 T6358 pobj adiposity,in
R4012 T6361 T6362 punct (,9B
R4013 T6362 T6356 parataxis 9B,increase
R4014 T6363 T6362 compound Figure,9B
R4015 T6364 T6362 punct ),9B
R4016 T6365 T6354 cc and,with
R4017 T6366 T6354 conj with,with
R4018 T6367 T6368 det the,labels
R4019 T6368 T6366 pobj labels,with
R4020 T6369 T6368 nummod two,labels
R4021 T6370 T6368 compound fluorochrome,labels
R4022 T6371 T6368 acl superimposed,labels
R4023 T6372 T6371 prep upon,superimposed
R4024 T6373 T6372 pobj one,upon
R4025 T6374 T6373 det another,one
R4026 T6375 T6376 punct (,9C
R4027 T6376 T6368 parataxis 9C,labels
R4028 T6377 T6376 compound Figure,9C
R4029 T6378 T6376 punct ),9C
R4030 T6379 T6339 punct .,showed
R4031 T6381 T6382 prep In,appeared
R4032 T6383 T6381 pobj contrast,In
R4033 T6384 T6382 punct ", ",appeared
R4034 T6385 T6386 det the,bones
R4035 T6386 T6382 nsubj bones,appeared
R4036 T6387 T6386 prep of,bones
R4037 T6388 T6389 nummod 12,month
R4038 T6389 T6391 npadvmod month,old
R4039 T6390 T6389 punct -,month
R4040 T6391 T6393 amod old,mice
R4041 T6392 T6391 punct -,old
R4042 T6393 T6387 pobj mice,of
R4043 T6394 T6393 nmod Sam68,mice
R4044 T6395 T6394 punct −,Sam68
R4045 T6396 T6394 punct /,Sam68
R4046 T6397 T6394 punct −,Sam68
R4047 T6398 T6382 oprd similar,appeared
R4048 T6399 T6398 prep to,similar
R4049 T6400 T6399 pobj those,to
R4050 T6401 T6400 prep of,those
R4051 T6402 T6403 det the,mice
R4052 T6403 T6401 pobj mice,of
R4053 T6404 T6405 nummod 4,month
R4054 T6405 T6407 npadvmod month,old
R4055 T6406 T6405 punct -,month
R4056 T6407 T6403 amod old,mice
R4057 T6408 T6407 punct -,old
R4058 T6409 T6403 prep of,mice
R4059 T6410 T6411 det either,genotype
R4060 T6411 T6409 pobj genotype,of
R4061 T6412 T6382 punct .,appeared
R4062 T6414 T6415 det These,data
R4063 T6415 T6416 nsubj data,demonstrate
R4064 T6417 T6418 mark that,regulates
R4065 T6418 T6416 ccomp regulates,demonstrate
R4066 T6419 T6418 nsubj Sam68,regulates
R4067 T6420 T6421 det the,differentiation
R4068 T6421 T6418 dobj differentiation,regulates
R4069 T6422 T6421 prep of,differentiation
R4070 T6423 T6424 nmod bone,marrow
R4071 T6424 T6425 nmod marrow,cells
R4072 T6425 T6422 pobj cells,of
R4073 T6426 T6425 amod mesenchymal,cells
R4074 T6427 T6428 aux to,promote
R4075 T6428 T6418 advcl promote,regulates
R4076 T6429 T6430 compound adipocyte,differentiation
R4077 T6430 T6428 dobj differentiation,promote
R4078 T6431 T6428 cc and,promote
R4079 T6432 T6428 conj inhibit,promote
R4080 T6433 T6434 compound osteoblast,differentiation
R4081 T6434 T6432 dobj differentiation,inhibit
R4082 T6435 T6428 prep in,promote
R4083 T6436 T6437 amod aging,bone
R4084 T6437 T6435 pobj bone,in
R4085 T6438 T6416 punct .,demonstrate