| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T11242 |
0-3 |
IN |
denotes |
For |
| T11244 |
4-14 |
JJ |
denotes |
osteogenic |
| T11245 |
15-30 |
NN |
denotes |
differentiation |
| T11246 |
30-32 |
, |
denotes |
, |
| T11247 |
32-39 |
NN |
denotes |
C3HT101 |
| T11249 |
39-40 |
HYPH |
denotes |
/ |
| T11250 |
40-41 |
CD |
denotes |
2 |
| T11251 |
42-43 |
-LRB- |
denotes |
( |
| T11252 |
43-47 |
NN |
denotes |
ATCC |
| T11253 |
47-48 |
-RRB- |
denotes |
) |
| T11248 |
49-54 |
NNS |
denotes |
cells |
| T11254 |
55-59 |
VBD |
denotes |
were |
| T11243 |
60-69 |
VBN |
denotes |
incubated |
| T11255 |
70-72 |
IN |
denotes |
in |
| T11256 |
73-75 |
CD |
denotes |
48 |
| T11258 |
75-76 |
HYPH |
denotes |
- |
| T11257 |
76-80 |
NN |
denotes |
well |
| T11260 |
81-87 |
NN |
denotes |
tissue |
| T11261 |
88-95 |
NN |
denotes |
culture |
| T11259 |
96-102 |
NNS |
denotes |
plates |
| T11262 |
103-105 |
IN |
denotes |
at |
| T11263 |
106-110 |
CD |
denotes |
1.25 |
| T11265 |
111-112 |
SYM |
denotes |
× |
| T11264 |
113-116 |
CD |
denotes |
104 |
| T11266 |
117-122 |
NNS |
denotes |
cells |
| T11267 |
122-123 |
SYM |
denotes |
/ |
| T11268 |
123-126 |
NN |
denotes |
cm2 |
| T11269 |
127-128 |
. |
denotes |
. |
| T11270 |
128-269 |
sentence |
denotes |
After 24-h incubation, the culture media were changed to fresh media containing 300 ng/ml BMP-2 (Sigma, St. Louis, Missouri, United States). |
| T11271 |
129-134 |
IN |
denotes |
After |
| T11273 |
135-137 |
CD |
denotes |
24 |
| T11275 |
137-138 |
HYPH |
denotes |
- |
| T11274 |
138-139 |
NN |
denotes |
h |
| T11276 |
140-150 |
NN |
denotes |
incubation |
| T11277 |
150-152 |
, |
denotes |
, |
| T11278 |
152-155 |
DT |
denotes |
the |
| T11280 |
156-163 |
NN |
denotes |
culture |
| T11279 |
164-169 |
NNS |
denotes |
media |
| T11281 |
170-174 |
VBD |
denotes |
were |
| T11272 |
175-182 |
VBN |
denotes |
changed |
| T11282 |
183-185 |
IN |
denotes |
to |
| T11283 |
186-191 |
JJ |
denotes |
fresh |
| T11284 |
192-197 |
NNS |
denotes |
media |
| T11285 |
198-208 |
VBG |
denotes |
containing |
| T11286 |
209-212 |
CD |
denotes |
300 |
| T11287 |
213-215 |
NN |
denotes |
ng |
| T11289 |
215-216 |
SYM |
denotes |
/ |
| T11290 |
216-218 |
NN |
denotes |
ml |
| T11288 |
219-222 |
NN |
denotes |
BMP |
| T11291 |
222-223 |
HYPH |
denotes |
- |
| T11292 |
223-224 |
CD |
denotes |
2 |
| T11293 |
225-226 |
-LRB- |
denotes |
( |
| T11294 |
226-231 |
NNP |
denotes |
Sigma |
| T11295 |
231-233 |
, |
denotes |
, |
| T11296 |
233-236 |
NNP |
denotes |
St. |
| T11297 |
237-242 |
NNP |
denotes |
Louis |
| T11298 |
242-244 |
, |
denotes |
, |
| T11299 |
244-252 |
NNP |
denotes |
Missouri |
| T11300 |
252-254 |
, |
denotes |
, |
| T11301 |
254-260 |
NNP |
denotes |
United |
| T11302 |
261-267 |
NNP |
denotes |
States |
| T11303 |
267-268 |
-RRB- |
denotes |
) |
| T11304 |
268-269 |
. |
denotes |
. |
| T11305 |
269-440 |
sentence |
denotes |
Total cellular RNA was prepared as described above, and osteocalcin, β-actin, and GAPDH gene expression at indicated times after BMP-2 treatment was quantified by RT-PCR. |
| T11306 |
270-275 |
JJ |
denotes |
Total |
| T11308 |
276-284 |
JJ |
denotes |
cellular |
| T11307 |
285-288 |
NN |
denotes |
RNA |
| T11310 |
289-292 |
VBD |
denotes |
was |
| T11309 |
293-301 |
VBN |
denotes |
prepared |
| T11311 |
302-304 |
IN |
denotes |
as |
| T11312 |
305-314 |
VBN |
denotes |
described |
| T11313 |
315-320 |
RB |
denotes |
above |
| T11314 |
320-322 |
, |
denotes |
, |
| T11315 |
322-325 |
CC |
denotes |
and |
| T11316 |
326-337 |
NN |
denotes |
osteocalcin |
| T11318 |
337-339 |
, |
denotes |
, |
| T11319 |
339-340 |
NN |
denotes |
β |
| T11321 |
340-341 |
HYPH |
denotes |
- |
| T11320 |
341-346 |
NN |
denotes |
actin |
| T11322 |
346-348 |
, |
denotes |
, |
| T11323 |
348-351 |
CC |
denotes |
and |
| T11324 |
352-357 |
NN |
denotes |
GAPDH |
| T11325 |
358-362 |
NN |
denotes |
gene |
| T11317 |
363-373 |
NN |
denotes |
expression |
| T11327 |
374-376 |
IN |
denotes |
at |
| T11328 |
377-386 |
VBN |
denotes |
indicated |
| T11329 |
387-392 |
NNS |
denotes |
times |
| T11330 |
393-398 |
IN |
denotes |
after |
| T11331 |
399-402 |
NN |
denotes |
BMP |
| T11333 |
402-403 |
HYPH |
denotes |
- |
| T11334 |
403-404 |
CD |
denotes |
2 |
| T11332 |
405-414 |
NN |
denotes |
treatment |
| T11335 |
415-418 |
VBD |
denotes |
was |
| T11326 |
419-429 |
VBN |
denotes |
quantified |
| T11336 |
430-432 |
IN |
denotes |
by |
| T11337 |
433-435 |
NN |
denotes |
RT |
| T11339 |
435-436 |
HYPH |
denotes |
- |
| T11338 |
436-439 |
NN |
denotes |
PCR |
| T11340 |
439-440 |
. |
denotes |
. |
| R7250 |
T11242 |
T11243 |
prep |
For,incubated |
| R7251 |
T11244 |
T11245 |
amod |
osteogenic,differentiation |
| R7252 |
T11245 |
T11242 |
pobj |
differentiation,For |
| R7253 |
T11246 |
T11243 |
punct |
", ",incubated |
| R7254 |
T11247 |
T11248 |
nmod |
C3HT101,cells |
| R7255 |
T11248 |
T11243 |
nsubjpass |
cells,incubated |
| R7256 |
T11249 |
T11247 |
punct |
/,C3HT101 |
| R7257 |
T11250 |
T11247 |
nummod |
2,C3HT101 |
| R7258 |
T11251 |
T11252 |
punct |
(,ATCC |
| R7259 |
T11252 |
T11247 |
parataxis |
ATCC,C3HT101 |
| R7260 |
T11253 |
T11252 |
punct |
),ATCC |
| R7261 |
T11254 |
T11243 |
auxpass |
were,incubated |
| R7262 |
T11255 |
T11243 |
prep |
in,incubated |
| R7263 |
T11256 |
T11257 |
nummod |
48,well |
| R7264 |
T11257 |
T11259 |
compound |
well,plates |
| R7265 |
T11258 |
T11257 |
punct |
-,well |
| R7266 |
T11259 |
T11255 |
pobj |
plates,in |
| R7267 |
T11260 |
T11259 |
compound |
tissue,plates |
| R7268 |
T11261 |
T11259 |
compound |
culture,plates |
| R7269 |
T11262 |
T11243 |
prep |
at,incubated |
| R7270 |
T11263 |
T11264 |
quantmod |
1.25,104 |
| R7271 |
T11264 |
T11266 |
nummod |
104,cells |
| R7272 |
T11265 |
T11264 |
punct |
×,104 |
| R7273 |
T11266 |
T11262 |
pobj |
cells,at |
| R7274 |
T11267 |
T11268 |
punct |
/,cm2 |
| R7275 |
T11268 |
T11266 |
prep |
cm2,cells |
| R7276 |
T11269 |
T11243 |
punct |
.,incubated |
| R7277 |
T11271 |
T11272 |
prep |
After,changed |
| R7278 |
T11273 |
T11274 |
nummod |
24,h |
| R7279 |
T11274 |
T11276 |
compound |
h,incubation |
| R7280 |
T11275 |
T11274 |
punct |
-,h |
| R7281 |
T11276 |
T11271 |
pobj |
incubation,After |
| R7282 |
T11277 |
T11272 |
punct |
", ",changed |
| R7283 |
T11278 |
T11279 |
det |
the,media |
| R7284 |
T11279 |
T11272 |
nsubjpass |
media,changed |
| R7285 |
T11280 |
T11279 |
compound |
culture,media |
| R7286 |
T11281 |
T11272 |
auxpass |
were,changed |
| R7287 |
T11282 |
T11272 |
prep |
to,changed |
| R7288 |
T11283 |
T11284 |
amod |
fresh,media |
| R7289 |
T11284 |
T11282 |
pobj |
media,to |
| R7290 |
T11285 |
T11284 |
acl |
containing,media |
| R7291 |
T11286 |
T11287 |
nummod |
300,ng |
| R7292 |
T11287 |
T11288 |
nmod |
ng,BMP |
| R7293 |
T11288 |
T11285 |
dobj |
BMP,containing |
| R7294 |
T11289 |
T11290 |
punct |
/,ml |
| R7295 |
T11290 |
T11287 |
prep |
ml,ng |
| R7296 |
T11291 |
T11288 |
punct |
-,BMP |
| R7297 |
T11292 |
T11288 |
nummod |
2,BMP |
| R7298 |
T11293 |
T11294 |
punct |
(,Sigma |
| R7299 |
T11294 |
T11288 |
parataxis |
Sigma,BMP |
| R7300 |
T11295 |
T11294 |
punct |
", ",Sigma |
| R7301 |
T11296 |
T11297 |
compound |
St.,Louis |
| R7302 |
T11297 |
T11294 |
npadvmod |
Louis,Sigma |
| R7303 |
T11298 |
T11294 |
punct |
", ",Sigma |
| R7304 |
T11299 |
T11294 |
npadvmod |
Missouri,Sigma |
| R7305 |
T11300 |
T11294 |
punct |
", ",Sigma |
| R7306 |
T11301 |
T11302 |
compound |
United,States |
| R7307 |
T11302 |
T11294 |
npadvmod |
States,Sigma |
| R7308 |
T11303 |
T11294 |
punct |
),Sigma |
| R7309 |
T11304 |
T11272 |
punct |
.,changed |
| R7310 |
T11306 |
T11307 |
amod |
Total,RNA |
| R7311 |
T11307 |
T11309 |
nsubjpass |
RNA,prepared |
| R7312 |
T11308 |
T11307 |
amod |
cellular,RNA |
| R7313 |
T11310 |
T11309 |
auxpass |
was,prepared |
| R7314 |
T11311 |
T11312 |
mark |
as,described |
| R7315 |
T11312 |
T11309 |
advcl |
described,prepared |
| R7316 |
T11313 |
T11312 |
advmod |
above,described |
| R7317 |
T11314 |
T11309 |
punct |
", ",prepared |
| R7318 |
T11315 |
T11309 |
cc |
and,prepared |
| R7319 |
T11316 |
T11317 |
nmod |
osteocalcin,expression |
| R7320 |
T11317 |
T11326 |
nsubjpass |
expression,quantified |
| R7321 |
T11318 |
T11316 |
punct |
", ",osteocalcin |
| R7322 |
T11319 |
T11320 |
compound |
β,actin |
| R7323 |
T11320 |
T11316 |
conj |
actin,osteocalcin |
| R7324 |
T11321 |
T11320 |
punct |
-,actin |
| R7325 |
T11322 |
T11320 |
punct |
", ",actin |
| R7326 |
T11323 |
T11320 |
cc |
and,actin |
| R7327 |
T11324 |
T11320 |
conj |
GAPDH,actin |
| R7328 |
T11325 |
T11317 |
compound |
gene,expression |
| R7329 |
T11326 |
T11309 |
conj |
quantified,prepared |
| R7330 |
T11327 |
T11317 |
prep |
at,expression |
| R7331 |
T11328 |
T11329 |
amod |
indicated,times |
| R7332 |
T11329 |
T11327 |
pobj |
times,at |
| R7333 |
T11330 |
T11329 |
prep |
after,times |
| R7334 |
T11331 |
T11332 |
nmod |
BMP,treatment |
| R7335 |
T11332 |
T11330 |
pobj |
treatment,after |
| R7336 |
T11333 |
T11331 |
punct |
-,BMP |
| R7337 |
T11334 |
T11331 |
nummod |
2,BMP |
| R7338 |
T11335 |
T11326 |
auxpass |
was,quantified |
| R7339 |
T11336 |
T11326 |
prep |
by,quantified |
| R7340 |
T11337 |
T11338 |
compound |
RT,PCR |
| R7341 |
T11338 |
T11336 |
pobj |
PCR,by |
| R7342 |
T11339 |
T11338 |
punct |
-,PCR |
| R7343 |
T11340 |
T11326 |
punct |
.,quantified |
