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PMC:1315279 / 40084-41914 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T9293 0-10 NN denotes Generation
T9294 11-13 IN denotes of
T9295 14-18 NNS denotes mice
T9296 19-23 IN denotes with
T9297 24-32 VBN denotes targeted
T9298 33-43 NN denotes disruption
T9299 44-46 IN denotes of
T9300 47-50 DT denotes the
T9302 51-56 NN denotes sam68
T9301 57-61 NN denotes gene
T9303 61-62 . denotes .
T9304 62-203 sentence denotes A λ bacteriophage clone encompassing Sam68 exons 3 to 9 was isolated from a 129/SvJ genomic library using full-length Sam68 cDNA as a probe.
T9305 63-64 DT denotes A
T9307 65-66 NN denotes λ
T9308 67-80 NN denotes bacteriophage
T9306 81-86 NN denotes clone
T9310 87-99 VBG denotes encompassing
T9311 100-105 NN denotes Sam68
T9313 106-111 NNS denotes exons
T9312 112-113 CD denotes 3
T9314 114-116 IN denotes to
T9315 117-118 CD denotes 9
T9316 119-122 VBD denotes was
T9309 123-131 VBN denotes isolated
T9317 132-136 IN denotes from
T9318 137-138 DT denotes a
T9320 139-142 CD denotes 129
T9322 142-143 HYPH denotes /
T9321 143-146 NN denotes SvJ
T9323 147-154 JJ denotes genomic
T9319 155-162 NN denotes library
T9324 163-168 VBG denotes using
T9325 169-173 JJ denotes full
T9327 173-174 HYPH denotes -
T9326 174-180 NN denotes length
T9329 181-186 NN denotes Sam68
T9328 187-191 NN denotes cDNA
T9330 192-194 IN denotes as
T9331 195-196 DT denotes a
T9332 197-202 NN denotes probe
T9333 202-203 . denotes .
T9334 203-413 sentence denotes XbaI-digested Sam68 genomic DNA fragments of 4 kb (encompassing exon 4 and part of exon 5) and 3kb (spanning part of exon 5 and exon 6) were subcloned in Bluescript SK resulting in pBS4 and pBS3, respectively.
T9335 204-208 NN denotes XbaI
T9337 208-209 HYPH denotes -
T9336 209-217 VBN denotes digested
T9339 218-223 NN denotes Sam68
T9340 224-231 JJ denotes genomic
T9341 232-235 NN denotes DNA
T9338 236-245 NNS denotes fragments
T9343 246-248 IN denotes of
T9344 249-250 CD denotes 4
T9345 251-253 NN denotes kb
T9346 254-255 -LRB- denotes (
T9347 255-267 VBG denotes encompassing
T9348 268-272 NN denotes exon
T9349 273-274 CD denotes 4
T9350 275-278 CC denotes and
T9351 279-283 NN denotes part
T9352 284-286 IN denotes of
T9353 287-291 NN denotes exon
T9354 292-293 CD denotes 5
T9355 293-294 -RRB- denotes )
T9356 295-298 CC denotes and
T9357 299-302 NN denotes 3kb
T9358 303-304 -LRB- denotes (
T9359 304-312 VBG denotes spanning
T9360 313-317 NN denotes part
T9361 318-320 IN denotes of
T9362 321-325 NN denotes exon
T9363 326-327 CD denotes 5
T9364 328-331 CC denotes and
T9365 332-336 NN denotes exon
T9366 337-338 CD denotes 6
T9367 338-339 -RRB- denotes )
T9368 340-344 VBD denotes were
T9342 345-354 VBN denotes subcloned
T9369 355-357 IN denotes in
T9370 358-368 NN denotes Bluescript
T9371 369-371 NN denotes SK
T9372 372-381 VBG denotes resulting
T9373 382-384 IN denotes in
T9374 385-389 NN denotes pBS4
T9375 390-393 CC denotes and
T9376 394-398 NN denotes pBS3
T9377 398-400 , denotes ,
T9378 400-412 RB denotes respectively
T9379 412-413 . denotes .
T9380 413-560 sentence denotes A DNA fragment was amplified from pBS4 with the following oligonucleotides (5′-AAT GTC TAG AAA CAA CTC ATA TAC AGA C-3′) and the universal primer.
T9381 414-415 DT denotes A
T9383 416-419 NN denotes DNA
T9382 420-428 NN denotes fragment
T9385 429-432 VBD denotes was
T9384 433-442 VBN denotes amplified
T9386 443-447 IN denotes from
T9387 448-452 NN denotes pBS4
T9388 453-457 IN denotes with
T9389 458-461 DT denotes the
T9391 462-471 JJ denotes following
T9390 472-488 NNS denotes oligonucleotides
T9392 489-490 -LRB- denotes (
T9393 490-491 CD denotes 5
T9394 491-492 SYM denotes ′
T9395 492-493 HYPH denotes -
T9396 493-496 NN denotes AAT
T9398 497-500 NN denotes GTC
T9399 501-504 NN denotes TAG
T9400 505-508 NN denotes AAA
T9401 509-512 NN denotes CAA
T9402 513-516 NN denotes CTC
T9403 517-520 NN denotes ATA
T9404 521-524 NN denotes TAC
T9405 525-528 NN denotes AGA
T9397 529-530 NN denotes C
T9406 530-531 HYPH denotes -
T9407 531-532 CD denotes 3
T9408 532-533 SYM denotes ′
T9409 533-534 -RRB- denotes )
T9410 535-538 CC denotes and
T9411 539-542 DT denotes the
T9413 543-552 JJ denotes universal
T9412 553-559 NN denotes primer
T9414 559-560 . denotes .
T9415 560-711 sentence denotes The XbaI-digested 1-kb DNA fragment was subcloned in the XbaI site of pPNT (a gift from Andrew Karaplis, McGill University, Montréal, Quebec, Canada).
T9416 561-564 DT denotes The
T9418 565-569 NN denotes XbaI
T9420 569-570 HYPH denotes -
T9419 570-578 VBN denotes digested
T9421 579-580 CD denotes 1
T9423 580-581 HYPH denotes -
T9422 581-583 NN denotes kb
T9424 584-587 NN denotes DNA
T9417 588-596 NN denotes fragment
T9426 597-600 VBD denotes was
T9425 601-610 VBN denotes subcloned
T9427 611-613 IN denotes in
T9428 614-617 DT denotes the
T9430 618-622 NN denotes XbaI
T9429 623-627 NN denotes site
T9431 628-630 IN denotes of
T9432 631-635 NN denotes pPNT
T9433 636-637 -LRB- denotes (
T9435 637-638 DT denotes a
T9434 639-643 NN denotes gift
T9436 644-648 IN denotes from
T9437 649-655 NNP denotes Andrew
T9438 656-664 NNP denotes Karaplis
T9439 664-666 , denotes ,
T9440 666-672 NNP denotes McGill
T9441 673-683 NNP denotes University
T9442 683-685 , denotes ,
T9443 685-693 NNP denotes Montréal
T9444 693-695 , denotes ,
T9445 695-701 NNP denotes Quebec
T9446 701-703 , denotes ,
T9447 703-709 NNP denotes Canada
T9448 709-710 -RRB- denotes )
T9449 710-711 . denotes .
T9450 711-925 sentence denotes The 3-kb fragment from pBS3 was amplified by PCR with (5′-GGG ATG CGG CCG CTC TAG AAT TGT CCT ACT TGA ACG G-3') and (5′-CGG TGG CGG CCG CTG TCG ACC TGA GTA ACA TTT CTT A-3′) and subcloned in the NotI site of pPNT.
T9451 712-715 DT denotes The
T9453 716-717 CD denotes 3
T9455 717-718 HYPH denotes -
T9454 718-720 NN denotes kb
T9452 721-729 NN denotes fragment
T9457 730-734 IN denotes from
T9458 735-739 NN denotes pBS3
T9459 740-743 VBD denotes was
T9456 744-753 VBN denotes amplified
T9460 754-756 IN denotes by
T9461 757-760 NN denotes PCR
T9462 761-765 IN denotes with
T9463 766-767 -LRB- denotes (
T9464 767-768 CD denotes 5
T9465 768-769 SYM denotes ′
T9466 769-770 HYPH denotes -
T9467 770-773 NN denotes GGG
T9469 774-777 NN denotes ATG
T9470 778-781 NN denotes CGG
T9471 782-785 NN denotes CCG
T9472 786-789 NN denotes CTC
T9473 790-793 NN denotes TAG
T9474 794-797 NN denotes AAT
T9475 798-801 NN denotes TGT
T9476 802-805 NN denotes CCT
T9477 806-809 NN denotes ACT
T9478 810-813 NN denotes TGA
T9479 814-817 NN denotes ACG
T9468 818-819 NN denotes G
T9480 819-820 HYPH denotes -
T9481 820-821 CD denotes 3
T9482 821-822 SYM denotes '
T9483 822-823 -RRB- denotes )
T9484 824-827 CC denotes and
T9485 828-829 -LRB- denotes (
T9486 829-830 CD denotes 5
T9487 830-831 SYM denotes ′
T9488 831-832 HYPH denotes -
T9489 832-835 NN denotes CGG
T9491 836-839 NN denotes TGG
T9492 840-843 NN denotes CGG
T9493 844-847 NN denotes CCG
T9494 848-851 NN denotes CTG
T9495 852-855 NN denotes TCG
T9496 856-859 NN denotes ACC
T9497 860-863 NN denotes TGA
T9498 864-867 NN denotes GTA
T9499 868-871 NN denotes ACA
T9500 872-875 NN denotes TTT
T9501 876-879 NN denotes CTT
T9490 880-881 NN denotes A
T9502 881-882 HYPH denotes -
T9503 882-883 CD denotes 3
T9504 883-884 SYM denotes ′
T9505 884-885 -RRB- denotes )
T9506 886-889 CC denotes and
T9507 890-899 VBN denotes subcloned
T9508 900-902 IN denotes in
T9509 903-906 DT denotes the
T9511 907-911 NN denotes NotI
T9510 912-916 NN denotes site
T9512 917-919 IN denotes of
T9513 920-924 NN denotes pPNT
T9514 924-925 . denotes .
T9515 925-1033 sentence denotes The targeting vector pPNT-Sam68 replaces exon 4 and part of exon 5 with a neomycin-resistant gene cassette.
T9516 926-929 DT denotes The
T9518 930-939 NN denotes targeting
T9517 940-946 NN denotes vector
T9520 947-951 NN denotes pPNT
T9522 951-952 HYPH denotes -
T9521 952-957 NN denotes Sam68
T9519 958-966 VBZ denotes replaces
T9523 967-971 NN denotes exon
T9524 972-973 CD denotes 4
T9525 974-977 CC denotes and
T9526 978-982 NN denotes part
T9527 983-985 IN denotes of
T9528 986-990 NN denotes exon
T9529 991-992 CD denotes 5
T9530 993-997 IN denotes with
T9531 998-999 DT denotes a
T9533 1000-1008 NN denotes neomycin
T9535 1008-1009 HYPH denotes -
T9534 1009-1018 JJ denotes resistant
T9536 1019-1023 NN denotes gene
T9532 1024-1032 NN denotes cassette
T9537 1032-1033 . denotes .
T9538 1033-1190 sentence denotes An SalI site was introduced at the 3′ end of the 3-kb DNA fragment and was used to linearize the plasmid for electroporation into embryonic stem (ES) cells.
T9539 1034-1036 DT denotes An
T9541 1037-1041 NN denotes SalI
T9540 1042-1046 NN denotes site
T9543 1047-1050 VBD denotes was
T9542 1051-1061 VBN denotes introduced
T9544 1062-1064 IN denotes at
T9545 1065-1068 DT denotes the
T9547 1069-1070 CD denotes 3
T9548 1070-1071 SYM denotes ′
T9546 1072-1075 NN denotes end
T9549 1076-1078 IN denotes of
T9550 1079-1082 DT denotes the
T9552 1083-1084 CD denotes 3
T9554 1084-1085 HYPH denotes -
T9553 1085-1087 NN denotes kb
T9555 1088-1091 NN denotes DNA
T9551 1092-1100 NN denotes fragment
T9556 1101-1104 CC denotes and
T9557 1105-1108 VBD denotes was
T9558 1109-1113 VBN denotes used
T9559 1114-1116 TO denotes to
T9560 1117-1126 VB denotes linearize
T9561 1127-1130 DT denotes the
T9562 1131-1138 NN denotes plasmid
T9563 1139-1142 IN denotes for
T9564 1143-1158 NN denotes electroporation
T9565 1159-1163 IN denotes into
T9566 1164-1173 JJ denotes embryonic
T9567 1174-1178 NN denotes stem
T9569 1179-1180 -LRB- denotes (
T9570 1180-1182 NN denotes ES
T9571 1182-1183 -RRB- denotes )
T9568 1184-1189 NNS denotes cells
T9572 1189-1190 . denotes .
T9573 1190-1343 sentence denotes Approximately 1,000 ES colonies were screened and two clones were identified that contained the Sam68 mutant allele, as determined by Southern blotting.
T9574 1191-1204 RB denotes Approximately
T9575 1205-1210 CD denotes 1,000
T9577 1211-1213 NN denotes ES
T9576 1214-1222 NNS denotes colonies
T9579 1223-1227 VBD denotes were
T9578 1228-1236 VBN denotes screened
T9580 1237-1240 CC denotes and
T9581 1241-1244 CD denotes two
T9582 1245-1251 NNS denotes clones
T9584 1252-1256 VBD denotes were
T9583 1257-1267 VBN denotes identified
T9585 1268-1272 WDT denotes that
T9586 1273-1282 VBD denotes contained
T9587 1283-1286 DT denotes the
T9588 1287-1292 NN denotes Sam68
T9590 1293-1299 NN denotes mutant
T9589 1300-1306 NN denotes allele
T9591 1306-1308 , denotes ,
T9592 1308-1310 IN denotes as
T9593 1311-1321 VBN denotes determined
T9594 1322-1324 IN denotes by
T9595 1325-1333 NNP denotes Southern
T9596 1334-1342 NN denotes blotting
T9597 1342-1343 . denotes .
T9598 1343-1536 sentence denotes Targeted ES cells were injected into 3.5-day-old BALB/c blastocysts and were transferred into CD-1 foster mothers, and animals classified as chimeras by coat color were mated with BALB/c mice.
T9599 1344-1352 VBN denotes Targeted
T9601 1353-1355 NN denotes ES
T9600 1356-1361 NNS denotes cells
T9603 1362-1366 VBD denotes were
T9602 1367-1375 VBN denotes injected
T9604 1376-1380 IN denotes into
T9605 1381-1384 CD denotes 3.5
T9607 1384-1385 HYPH denotes -
T9606 1385-1388 NN denotes day
T9609 1388-1389 HYPH denotes -
T9608 1389-1392 JJ denotes old
T9611 1393-1397 NN denotes BALB
T9613 1397-1398 HYPH denotes /
T9612 1398-1399 NN denotes c
T9610 1400-1411 NNS denotes blastocysts
T9614 1412-1415 CC denotes and
T9615 1416-1420 VBD denotes were
T9616 1421-1432 VBN denotes transferred
T9617 1433-1437 IN denotes into
T9618 1438-1440 NN denotes CD
T9620 1440-1441 HYPH denotes -
T9621 1441-1442 CD denotes 1
T9622 1443-1449 NN denotes foster
T9619 1450-1457 NNS denotes mothers
T9623 1457-1459 , denotes ,
T9624 1459-1462 CC denotes and
T9625 1463-1470 NNS denotes animals
T9627 1471-1481 VBN denotes classified
T9628 1482-1484 IN denotes as
T9629 1485-1493 NNS denotes chimeras
T9630 1494-1496 IN denotes by
T9631 1497-1501 NN denotes coat
T9632 1502-1507 NN denotes color
T9633 1508-1512 VBD denotes were
T9626 1513-1518 VBN denotes mated
T9634 1519-1523 IN denotes with
T9635 1524-1528 NN denotes BALB
T9637 1528-1529 HYPH denotes /
T9636 1529-1530 NN denotes c
T9638 1531-1535 NNS denotes mice
T9639 1535-1536 . denotes .
T9640 1536-1624 sentence denotes Germ line transmission was achieved and the mice were maintained in C57BL/6 background.
T9641 1537-1541 NN denotes Germ
T9642 1542-1546 NN denotes line
T9643 1547-1559 NN denotes transmission
T9645 1560-1563 VBD denotes was
T9644 1564-1572 VBN denotes achieved
T9646 1573-1576 CC denotes and
T9647 1577-1580 DT denotes the
T9648 1581-1585 NNS denotes mice
T9650 1586-1590 VBD denotes were
T9649 1591-1601 VBN denotes maintained
T9651 1602-1604 IN denotes in
T9652 1605-1610 NN denotes C57BL
T9654 1610-1611 HYPH denotes /
T9655 1611-1612 CD denotes 6
T9653 1613-1623 NN denotes background
T9656 1623-1624 . denotes .
T9657 1624-1830 sentence denotes The mice used for this study represent mice that were backcrossed in C57BL/6 between three and eight generations; in addition, we maintained the mice in the 129/SvJ strain and observed a similar phenotype.
T9658 1625-1628 DT denotes The
T9659 1629-1633 NNS denotes mice
T9661 1634-1638 VBN denotes used
T9662 1639-1642 IN denotes for
T9663 1643-1647 DT denotes this
T9664 1648-1653 NN denotes study
T9660 1654-1663 VBP denotes represent
T9666 1664-1668 NNS denotes mice
T9667 1669-1673 WDT denotes that
T9669 1674-1678 VBD denotes were
T9668 1679-1690 VBN denotes backcrossed
T9670 1691-1693 IN denotes in
T9671 1694-1699 NN denotes C57BL
T9672 1699-1700 HYPH denotes /
T9673 1700-1701 CD denotes 6
T9674 1702-1709 IN denotes between
T9675 1710-1715 CD denotes three
T9677 1716-1719 CC denotes and
T9678 1720-1725 CD denotes eight
T9676 1726-1737 NNS denotes generations
T9679 1737-1738 : denotes ;
T9680 1739-1741 IN denotes in
T9681 1742-1750 NN denotes addition
T9682 1750-1752 , denotes ,
T9683 1752-1754 PRP denotes we
T9665 1755-1765 VBD denotes maintained
T9684 1766-1769 DT denotes the
T9685 1770-1774 NNS denotes mice
T9686 1775-1777 IN denotes in
T9687 1778-1781 DT denotes the
T9689 1782-1785 CD denotes 129
T9691 1785-1786 HYPH denotes /
T9690 1786-1789 NN denotes SvJ
T9688 1790-1796 NN denotes strain
T9692 1797-1800 CC denotes and
T9693 1801-1809 VBD denotes observed
T9694 1810-1811 DT denotes a
T9696 1812-1819 JJ denotes similar
T9695 1820-1829 NN denotes phenotype
T9697 1829-1830 . denotes .
R5772 T9294 T9293 prep of,Generation
R5773 T9295 T9294 pobj mice,of
R5774 T9296 T9295 prep with,mice
R5775 T9297 T9298 amod targeted,disruption
R5776 T9298 T9296 pobj disruption,with
R5777 T9299 T9298 prep of,disruption
R5778 T9300 T9301 det the,gene
R5779 T9301 T9299 pobj gene,of
R5780 T9302 T9301 compound sam68,gene
R5781 T9303 T9293 punct .,Generation
R5782 T9305 T9306 det A,clone
R5783 T9306 T9309 nsubjpass clone,isolated
R5784 T9307 T9306 compound λ,clone
R5785 T9308 T9306 compound bacteriophage,clone
R5786 T9310 T9306 acl encompassing,clone
R5787 T9311 T9312 nmod Sam68,3
R5788 T9312 T9310 dobj 3,encompassing
R5789 T9313 T9312 nmod exons,3
R5790 T9314 T9312 prep to,3
R5791 T9315 T9314 pobj 9,to
R5792 T9316 T9309 auxpass was,isolated
R5793 T9317 T9309 prep from,isolated
R5794 T9318 T9319 det a,library
R5795 T9319 T9317 pobj library,from
R5796 T9320 T9321 nummod 129,SvJ
R5797 T9321 T9319 nmod SvJ,library
R5798 T9322 T9321 punct /,SvJ
R5799 T9323 T9319 amod genomic,library
R5800 T9324 T9309 advcl using,isolated
R5801 T9325 T9326 amod full,length
R5802 T9326 T9328 compound length,cDNA
R5803 T9327 T9326 punct -,length
R5804 T9328 T9324 dobj cDNA,using
R5805 T9329 T9328 compound Sam68,cDNA
R5806 T9330 T9324 prep as,using
R5807 T9331 T9332 det a,probe
R5808 T9332 T9330 pobj probe,as
R5809 T9333 T9309 punct .,isolated
R5810 T9335 T9336 npadvmod XbaI,digested
R5811 T9336 T9338 amod digested,fragments
R5812 T9337 T9336 punct -,digested
R5813 T9338 T9342 nsubjpass fragments,subcloned
R5814 T9339 T9338 nmod Sam68,fragments
R5815 T9340 T9338 amod genomic,fragments
R5816 T9341 T9338 compound DNA,fragments
R5817 T9343 T9338 prep of,fragments
R5818 T9344 T9345 nummod 4,kb
R5819 T9345 T9343 pobj kb,of
R5820 T9346 T9347 punct (,encompassing
R5821 T9347 T9345 parataxis encompassing,kb
R5822 T9348 T9347 dobj exon,encompassing
R5823 T9349 T9348 nummod 4,exon
R5824 T9350 T9348 cc and,exon
R5825 T9351 T9348 conj part,exon
R5826 T9352 T9351 prep of,part
R5827 T9353 T9352 pobj exon,of
R5828 T9354 T9353 nummod 5,exon
R5829 T9355 T9347 punct ),encompassing
R5830 T9356 T9345 cc and,kb
R5831 T9357 T9345 conj 3kb,kb
R5832 T9358 T9359 punct (,spanning
R5833 T9359 T9357 parataxis spanning,3kb
R5834 T9360 T9359 dobj part,spanning
R5835 T9361 T9360 prep of,part
R5836 T9362 T9361 pobj exon,of
R5837 T9363 T9362 nummod 5,exon
R5838 T9364 T9360 cc and,part
R5839 T9365 T9360 conj exon,part
R5840 T9366 T9365 nummod 6,exon
R5841 T9367 T9359 punct ),spanning
R5842 T9368 T9342 auxpass were,subcloned
R5843 T9369 T9342 prep in,subcloned
R5844 T9370 T9371 compound Bluescript,SK
R5845 T9371 T9369 pobj SK,in
R5846 T9372 T9342 advcl resulting,subcloned
R5847 T9373 T9372 prep in,resulting
R5848 T9374 T9373 pobj pBS4,in
R5849 T9375 T9374 cc and,pBS4
R5850 T9376 T9374 conj pBS3,pBS4
R5851 T9377 T9372 punct ", ",resulting
R5852 T9378 T9372 advmod respectively,resulting
R5853 T9379 T9342 punct .,subcloned
R5854 T9381 T9382 det A,fragment
R5855 T9382 T9384 nsubjpass fragment,amplified
R5856 T9383 T9382 compound DNA,fragment
R5857 T9385 T9384 auxpass was,amplified
R5858 T9386 T9384 prep from,amplified
R5859 T9387 T9386 pobj pBS4,from
R5860 T9388 T9384 prep with,amplified
R5861 T9389 T9390 det the,oligonucleotides
R5862 T9390 T9388 pobj oligonucleotides,with
R5863 T9391 T9390 amod following,oligonucleotides
R5864 T9392 T9393 punct (,5
R5865 T9393 T9390 parataxis 5,oligonucleotides
R5866 T9394 T9393 punct ′,5
R5867 T9395 T9393 punct -,5
R5868 T9396 T9397 compound AAT,C
R5869 T9397 T9393 appos C,5
R5870 T9398 T9397 compound GTC,C
R5871 T9399 T9397 compound TAG,C
R5872 T9400 T9397 compound AAA,C
R5873 T9401 T9397 compound CAA,C
R5874 T9402 T9397 compound CTC,C
R5875 T9403 T9397 compound ATA,C
R5876 T9404 T9397 compound TAC,C
R5877 T9405 T9397 compound AGA,C
R5878 T9406 T9393 punct -,5
R5879 T9407 T9393 nummod 3,5
R5880 T9408 T9393 punct ′,5
R5881 T9409 T9393 punct ),5
R5882 T9410 T9390 cc and,oligonucleotides
R5883 T9411 T9412 det the,primer
R5884 T9412 T9390 conj primer,oligonucleotides
R5885 T9413 T9412 amod universal,primer
R5886 T9414 T9384 punct .,amplified
R5887 T9416 T9417 det The,fragment
R5888 T9417 T9425 nsubjpass fragment,subcloned
R5889 T9418 T9419 npadvmod XbaI,digested
R5890 T9419 T9417 amod digested,fragment
R5891 T9420 T9419 punct -,digested
R5892 T9421 T9422 nummod 1,kb
R5893 T9422 T9417 compound kb,fragment
R5894 T9423 T9422 punct -,kb
R5895 T9424 T9417 compound DNA,fragment
R5896 T9426 T9425 auxpass was,subcloned
R5897 T9427 T9425 prep in,subcloned
R5898 T9428 T9429 det the,site
R5899 T9429 T9427 pobj site,in
R5900 T9430 T9429 compound XbaI,site
R5901 T9431 T9429 prep of,site
R5902 T9432 T9431 pobj pPNT,of
R5903 T9433 T9434 punct (,gift
R5904 T9434 T9432 parataxis gift,pPNT
R5905 T9435 T9434 det a,gift
R5906 T9436 T9434 prep from,gift
R5907 T9437 T9438 compound Andrew,Karaplis
R5908 T9438 T9436 pobj Karaplis,from
R5909 T9439 T9438 punct ", ",Karaplis
R5910 T9440 T9441 compound McGill,University
R5911 T9441 T9438 npadvmod University,Karaplis
R5912 T9442 T9438 punct ", ",Karaplis
R5913 T9443 T9438 npadvmod Montréal,Karaplis
R5914 T9444 T9438 punct ", ",Karaplis
R5915 T9445 T9438 npadvmod Quebec,Karaplis
R5916 T9446 T9438 punct ", ",Karaplis
R5917 T9447 T9438 npadvmod Canada,Karaplis
R5918 T9448 T9434 punct ),gift
R5919 T9449 T9425 punct .,subcloned
R5920 T9451 T9452 det The,fragment
R5921 T9452 T9456 nsubjpass fragment,amplified
R5922 T9453 T9454 nummod 3,kb
R5923 T9454 T9452 compound kb,fragment
R5924 T9455 T9454 punct -,kb
R5925 T9457 T9452 prep from,fragment
R5926 T9458 T9457 pobj pBS3,from
R5927 T9459 T9456 auxpass was,amplified
R5928 T9460 T9456 prep by,amplified
R5929 T9461 T9460 pobj PCR,by
R5930 T9462 T9461 prep with,PCR
R5931 T9463 T9462 punct (,with
R5932 T9464 T9462 pobj 5,with
R5933 T9465 T9464 punct ′,5
R5934 T9466 T9464 punct -,5
R5935 T9467 T9468 compound GGG,G
R5936 T9468 T9464 appos G,5
R5937 T9469 T9468 compound ATG,G
R5938 T9470 T9468 compound CGG,G
R5939 T9471 T9468 compound CCG,G
R5940 T9472 T9468 compound CTC,G
R5941 T9473 T9468 compound TAG,G
R5942 T9474 T9468 compound AAT,G
R5943 T9475 T9468 compound TGT,G
R5944 T9476 T9468 compound CCT,G
R5945 T9477 T9468 compound ACT,G
R5946 T9478 T9468 compound TGA,G
R5947 T9479 T9468 compound ACG,G
R5948 T9480 T9464 punct -,5
R5949 T9481 T9464 nummod 3,5
R5950 T9482 T9464 punct ',5
R5951 T9483 T9464 punct ),5
R5952 T9484 T9464 cc and,5
R5953 T9485 T9486 punct (,5
R5954 T9486 T9464 conj 5,5
R5955 T9487 T9486 punct ′,5
R5956 T9488 T9486 punct -,5
R5957 T9489 T9490 compound CGG,A
R5958 T9490 T9486 appos A,5
R5959 T9491 T9490 compound TGG,A
R5960 T9492 T9490 compound CGG,A
R5961 T9493 T9490 compound CCG,A
R5962 T9494 T9490 compound CTG,A
R5963 T9495 T9490 compound TCG,A
R5964 T9496 T9490 compound ACC,A
R5965 T9497 T9490 compound TGA,A
R5966 T9498 T9490 compound GTA,A
R5967 T9499 T9490 compound ACA,A
R5968 T9500 T9490 compound TTT,A
R5969 T9501 T9490 compound CTT,A
R5970 T9502 T9486 punct -,5
R5971 T9503 T9486 nummod 3,5
R5972 T9504 T9486 punct ′,5
R5973 T9505 T9456 punct ),amplified
R5974 T9506 T9456 cc and,amplified
R5975 T9507 T9456 conj subcloned,amplified
R5976 T9508 T9507 prep in,subcloned
R5977 T9509 T9510 det the,site
R5978 T9510 T9508 pobj site,in
R5979 T9511 T9510 compound NotI,site
R5980 T9512 T9510 prep of,site
R5981 T9513 T9512 pobj pPNT,of
R5982 T9514 T9456 punct .,amplified
R5983 T9516 T9517 det The,vector
R5984 T9517 T9519 nsubj vector,replaces
R5985 T9518 T9517 compound targeting,vector
R5986 T9520 T9521 compound pPNT,Sam68
R5987 T9521 T9517 appos Sam68,vector
R5988 T9522 T9521 punct -,Sam68
R5989 T9523 T9519 dobj exon,replaces
R5990 T9524 T9523 nummod 4,exon
R5991 T9525 T9523 cc and,exon
R5992 T9526 T9523 conj part,exon
R5993 T9527 T9526 prep of,part
R5994 T9528 T9527 pobj exon,of
R5995 T9529 T9528 nummod 5,exon
R5996 T9530 T9519 prep with,replaces
R5997 T9531 T9532 det a,cassette
R5998 T9532 T9530 pobj cassette,with
R5999 T9533 T9534 npadvmod neomycin,resistant
R6000 T9534 T9532 amod resistant,cassette
R6001 T9535 T9534 punct -,resistant
R6002 T9536 T9532 compound gene,cassette
R6003 T9537 T9519 punct .,replaces
R6004 T9539 T9540 det An,site
R6005 T9540 T9542 nsubjpass site,introduced
R6006 T9541 T9540 compound SalI,site
R6007 T9543 T9542 auxpass was,introduced
R6008 T9544 T9542 prep at,introduced
R6009 T9545 T9546 det the,end
R6010 T9546 T9544 pobj end,at
R6011 T9547 T9546 nummod 3,end
R6012 T9548 T9547 punct ′,3
R6013 T9549 T9546 prep of,end
R6014 T9550 T9551 det the,fragment
R6015 T9551 T9549 pobj fragment,of
R6016 T9552 T9553 nummod 3,kb
R6017 T9553 T9551 compound kb,fragment
R6018 T9554 T9553 punct -,kb
R6019 T9555 T9551 compound DNA,fragment
R6020 T9556 T9542 cc and,introduced
R6021 T9557 T9558 auxpass was,used
R6022 T9558 T9542 conj used,introduced
R6023 T9559 T9560 aux to,linearize
R6024 T9560 T9558 advcl linearize,used
R6025 T9561 T9562 det the,plasmid
R6026 T9562 T9560 dobj plasmid,linearize
R6027 T9563 T9560 prep for,linearize
R6028 T9564 T9563 pobj electroporation,for
R6029 T9565 T9564 prep into,electroporation
R6030 T9566 T9567 amod embryonic,stem
R6031 T9567 T9568 nmod stem,cells
R6032 T9568 T9565 pobj cells,into
R6033 T9569 T9567 punct (,stem
R6034 T9570 T9567 appos ES,stem
R6035 T9571 T9568 punct ),cells
R6036 T9572 T9542 punct .,introduced
R6037 T9574 T9575 advmod Approximately,"1,000"
R6038 T9575 T9576 nummod "1,000",colonies
R6039 T9576 T9578 nsubjpass colonies,screened
R6040 T9577 T9576 compound ES,colonies
R6041 T9579 T9578 auxpass were,screened
R6042 T9580 T9578 cc and,screened
R6043 T9581 T9582 nummod two,clones
R6044 T9582 T9583 nsubjpass clones,identified
R6045 T9583 T9578 conj identified,screened
R6046 T9584 T9583 auxpass were,identified
R6047 T9585 T9586 dep that,contained
R6048 T9586 T9583 ccomp contained,identified
R6049 T9587 T9588 det the,Sam68
R6050 T9588 T9589 compound Sam68,allele
R6051 T9589 T9586 dobj allele,contained
R6052 T9590 T9589 compound mutant,allele
R6053 T9591 T9583 punct ", ",identified
R6054 T9592 T9593 mark as,determined
R6055 T9593 T9583 advcl determined,identified
R6056 T9594 T9593 prep by,determined
R6057 T9595 T9596 compound Southern,blotting
R6058 T9596 T9594 pobj blotting,by
R6059 T9597 T9583 punct .,identified
R6060 T9599 T9600 amod Targeted,cells
R6061 T9600 T9602 nsubjpass cells,injected
R6062 T9601 T9600 compound ES,cells
R6063 T9603 T9602 auxpass were,injected
R6064 T9604 T9602 prep into,injected
R6065 T9605 T9606 nummod 3.5,day
R6066 T9606 T9608 npadvmod day,old
R6067 T9607 T9606 punct -,day
R6068 T9608 T9610 amod old,blastocysts
R6069 T9609 T9608 punct -,old
R6070 T9610 T9604 pobj blastocysts,into
R6071 T9611 T9612 compound BALB,c
R6072 T9612 T9610 compound c,blastocysts
R6073 T9613 T9612 punct /,c
R6074 T9614 T9602 cc and,injected
R6075 T9615 T9616 auxpass were,transferred
R6076 T9616 T9602 conj transferred,injected
R6077 T9617 T9616 prep into,transferred
R6078 T9618 T9619 nmod CD,mothers
R6079 T9619 T9617 pobj mothers,into
R6080 T9620 T9618 punct -,CD
R6081 T9621 T9618 nummod 1,CD
R6082 T9622 T9619 compound foster,mothers
R6083 T9623 T9602 punct ", ",injected
R6084 T9624 T9602 cc and,injected
R6085 T9625 T9626 nsubjpass animals,mated
R6086 T9626 T9602 conj mated,injected
R6087 T9627 T9625 acl classified,animals
R6088 T9628 T9627 prep as,classified
R6089 T9629 T9628 pobj chimeras,as
R6090 T9630 T9627 prep by,classified
R6091 T9631 T9632 compound coat,color
R6092 T9632 T9630 pobj color,by
R6093 T9633 T9626 auxpass were,mated
R6094 T9634 T9626 prep with,mated
R6095 T9635 T9636 compound BALB,c
R6096 T9636 T9638 compound c,mice
R6097 T9637 T9636 punct /,c
R6098 T9638 T9634 pobj mice,with
R6099 T9639 T9626 punct .,mated
R6100 T9641 T9642 compound Germ,line
R6101 T9642 T9643 compound line,transmission
R6102 T9643 T9644 nsubjpass transmission,achieved
R6103 T9645 T9644 auxpass was,achieved
R6104 T9646 T9644 cc and,achieved
R6105 T9647 T9648 det the,mice
R6106 T9648 T9649 nsubjpass mice,maintained
R6107 T9649 T9644 conj maintained,achieved
R6108 T9650 T9649 auxpass were,maintained
R6109 T9651 T9649 prep in,maintained
R6110 T9652 T9653 nmod C57BL,background
R6111 T9653 T9651 pobj background,in
R6112 T9654 T9652 punct /,C57BL
R6113 T9655 T9652 nummod 6,C57BL
R6114 T9656 T9649 punct .,maintained
R6115 T9658 T9659 det The,mice
R6116 T9659 T9660 nsubj mice,represent
R6117 T9660 T9665 ccomp represent,maintained
R6118 T9661 T9659 acl used,mice
R6119 T9662 T9661 prep for,used
R6120 T9663 T9664 det this,study
R6121 T9664 T9662 pobj study,for
R6122 T9666 T9660 dobj mice,represent
R6123 T9667 T9668 dep that,backcrossed
R6124 T9668 T9666 relcl backcrossed,mice
R6125 T9669 T9668 auxpass were,backcrossed
R6126 T9670 T9668 prep in,backcrossed
R6127 T9671 T9670 pobj C57BL,in
R6128 T9672 T9671 punct /,C57BL
R6129 T9673 T9671 nummod 6,C57BL
R6130 T9674 T9675 quantmod between,three
R6131 T9675 T9676 nummod three,generations
R6132 T9676 T9668 npadvmod generations,backcrossed
R6133 T9677 T9675 cc and,three
R6134 T9678 T9675 conj eight,three
R6135 T9679 T9665 punct ;,maintained
R6136 T9680 T9665 prep in,maintained
R6137 T9681 T9680 pobj addition,in
R6138 T9682 T9665 punct ", ",maintained
R6139 T9683 T9665 nsubj we,maintained
R6140 T9684 T9685 det the,mice
R6141 T9685 T9665 dobj mice,maintained
R6142 T9686 T9685 prep in,mice
R6143 T9687 T9688 det the,strain
R6144 T9688 T9686 pobj strain,in
R6145 T9689 T9690 nummod 129,SvJ
R6146 T9690 T9688 compound SvJ,strain
R6147 T9691 T9690 punct /,SvJ
R6148 T9692 T9665 cc and,maintained
R6149 T9693 T9665 conj observed,maintained
R6150 T9694 T9695 det a,phenotype
R6151 T9695 T9693 dobj phenotype,observed
R6152 T9696 T9695 amod similar,phenotype
R6153 T9697 T9665 punct .,maintained