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PMC:1315279 / 3043-48409 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T1057 13-19 IN denotes During
T1059 20-28 JJ denotes skeletal
T1060 29-40 NN denotes development
T1061 40-42 , denotes ,
T1062 42-45 DT denotes the
T1064 46-54 JJ denotes anabolic
T1063 55-63 NN denotes activity
T1065 64-66 IN denotes of
T1066 67-78 NNS denotes osteoblasts
T1067 79-80 -LRB- denotes [
T1068 80-81 CD denotes 1
T1069 81-82 -RRB- denotes ]
T1070 83-85 VBZ denotes is
T1058 86-93 VBN denotes favored
T1071 94-98 IN denotes over
T1072 99-102 DT denotes the
T1074 103-112 JJ denotes catabolic
T1073 113-121 NN denotes activity
T1075 122-124 IN denotes of
T1076 125-136 NNS denotes osteoclasts
T1077 137-138 -LRB- denotes [
T1078 138-139 CD denotes 2
T1079 139-140 -RRB- denotes ]
T1080 140-142 , denotes ,
T1081 142-147 WDT denotes which
T1082 148-155 VBZ denotes results
T1083 156-158 IN denotes in
T1084 159-160 DT denotes a
T1086 161-164 JJ denotes net
T1085 165-169 NN denotes gain
T1087 170-172 IN denotes in
T1088 173-177 NN denotes bone
T1089 178-182 NN denotes mass
T1090 182-183 . denotes .
T1091 183-319 sentence denotes At skeletal maturity, bone mass is maintained through the balanced activity of osteoblasts and osteoclasts during the remodeling cycle.
T1092 184-186 IN denotes At
T1094 187-195 JJ denotes skeletal
T1095 196-204 NN denotes maturity
T1096 204-206 , denotes ,
T1097 206-210 NN denotes bone
T1098 211-215 NN denotes mass
T1099 216-218 VBZ denotes is
T1093 219-229 VBN denotes maintained
T1100 230-237 IN denotes through
T1101 238-241 DT denotes the
T1103 242-250 VBN denotes balanced
T1102 251-259 NN denotes activity
T1104 260-262 IN denotes of
T1105 263-274 NNS denotes osteoblasts
T1106 275-278 CC denotes and
T1107 279-290 NNS denotes osteoclasts
T1108 291-297 IN denotes during
T1109 298-301 DT denotes the
T1111 302-312 NN denotes remodeling
T1110 313-318 NN denotes cycle
T1112 318-319 . denotes .
T1113 319-459 sentence denotes During skeletal aging, there is a shift in the balance that favors osteoclast over osteoblast activity, which results in net bone loss [3].
T1114 320-326 IN denotes During
T1116 327-335 JJ denotes skeletal
T1117 336-341 NN denotes aging
T1118 341-343 , denotes ,
T1119 343-348 EX denotes there
T1115 349-351 VBZ denotes is
T1120 352-353 DT denotes a
T1121 354-359 NN denotes shift
T1122 360-362 IN denotes in
T1123 363-366 DT denotes the
T1124 367-374 NN denotes balance
T1125 375-379 WDT denotes that
T1126 380-386 VBZ denotes favors
T1127 387-397 NN denotes osteoclast
T1128 398-402 IN denotes over
T1129 403-413 NN denotes osteoblast
T1130 414-422 NN denotes activity
T1131 422-424 , denotes ,
T1132 424-429 WDT denotes which
T1133 430-437 VBZ denotes results
T1134 438-440 IN denotes in
T1135 441-444 JJ denotes net
T1137 445-449 NN denotes bone
T1136 450-454 NN denotes loss
T1138 455-456 -LRB- denotes [
T1139 456-457 CD denotes 3
T1140 457-458 -RRB- denotes ]
T1141 458-459 . denotes .
T1142 459-761 sentence denotes The amount and rate at which bone is gained during development and lost during aging are determined in large part by genetics [4–6] but also by physical activity and by alterations in the availability and response of bone cells to circulating hormones [7–9] and locally derived growth factors [10,11].
T1143 460-463 DT denotes The
T1144 464-470 NN denotes amount
T1146 471-474 CC denotes and
T1147 475-479 NN denotes rate
T1148 480-482 IN denotes at
T1150 483-488 WDT denotes which
T1151 489-493 NN denotes bone
T1152 494-496 VBZ denotes is
T1149 497-503 VBN denotes gained
T1153 504-510 IN denotes during
T1154 511-522 NN denotes development
T1155 523-526 CC denotes and
T1156 527-531 VBN denotes lost
T1157 532-538 IN denotes during
T1158 539-544 NN denotes aging
T1159 545-548 VBP denotes are
T1145 549-559 VBN denotes determined
T1160 560-562 IN denotes in
T1161 563-568 JJ denotes large
T1162 569-573 NN denotes part
T1163 574-576 IN denotes by
T1164 577-585 NN denotes genetics
T1165 586-587 -LRB- denotes [
T1166 587-588 CD denotes 4
T1167 588-589 SYM denotes –
T1168 589-590 CD denotes 6
T1169 590-591 -RRB- denotes ]
T1170 592-595 CC denotes but
T1171 596-600 RB denotes also
T1172 601-603 IN denotes by
T1173 604-612 JJ denotes physical
T1174 613-621 NN denotes activity
T1175 622-625 CC denotes and
T1176 626-628 IN denotes by
T1177 629-640 NNS denotes alterations
T1178 641-643 IN denotes in
T1179 644-647 DT denotes the
T1180 648-660 NN denotes availability
T1181 661-664 CC denotes and
T1182 665-673 NN denotes response
T1183 674-676 IN denotes of
T1184 677-681 NN denotes bone
T1185 682-687 NNS denotes cells
T1186 688-690 IN denotes to
T1187 691-702 VBG denotes circulating
T1188 703-711 NNS denotes hormones
T1189 712-713 -LRB- denotes [
T1190 713-714 CD denotes 7
T1191 714-715 SYM denotes –
T1192 715-716 CD denotes 9
T1193 716-717 -RRB- denotes ]
T1194 718-721 CC denotes and
T1195 722-729 RB denotes locally
T1196 730-737 VBN denotes derived
T1198 738-744 NN denotes growth
T1197 745-752 NNS denotes factors
T1199 753-754 -LRB- denotes [
T1201 754-756 CD denotes 10
T1202 756-757 , denotes ,
T1200 757-759 CD denotes 11
T1203 759-760 -RRB- denotes ]
T1204 760-761 . denotes .
T1205 761-950 sentence denotes Whereas genetic-based studies have provided novel insights into the pathways that regulate bone development [12–14], relatively little is known about the etiology of age-related bone loss.
T1206 762-769 IN denotes Whereas
T1208 770-777 JJ denotes genetic
T1210 777-778 HYPH denotes -
T1209 778-783 VBN denotes based
T1211 784-791 NNS denotes studies
T1212 792-796 VBP denotes have
T1207 797-805 VBN denotes provided
T1214 806-811 JJ denotes novel
T1215 812-820 NNS denotes insights
T1216 821-825 IN denotes into
T1217 826-829 DT denotes the
T1218 830-838 NNS denotes pathways
T1219 839-843 WDT denotes that
T1220 844-852 VBP denotes regulate
T1221 853-857 NN denotes bone
T1222 858-869 NN denotes development
T1223 870-871 -LRB- denotes [
T1224 871-873 CD denotes 12
T1225 873-874 SYM denotes –
T1226 874-876 CD denotes 14
T1227 876-877 -RRB- denotes ]
T1228 877-879 , denotes ,
T1229 879-889 RB denotes relatively
T1230 890-896 JJ denotes little
T1231 897-899 VBZ denotes is
T1213 900-905 VBN denotes known
T1232 906-911 IN denotes about
T1233 912-915 DT denotes the
T1234 916-924 NN denotes etiology
T1235 925-927 IN denotes of
T1236 928-931 NN denotes age
T1238 931-932 HYPH denotes -
T1237 932-939 VBN denotes related
T1240 940-944 NN denotes bone
T1239 945-949 NN denotes loss
T1241 949-950 . denotes .
T1242 950-1108 sentence denotes Increased bone resorption in elderly men and women is associated with a reduction in bone mass and an increase in circulating levels of bone biomarkers [15].
T1243 951-960 VBN denotes Increased
T1245 961-965 NN denotes bone
T1244 966-976 NN denotes resorption
T1247 977-979 IN denotes in
T1248 980-987 JJ denotes elderly
T1249 988-991 NNS denotes men
T1250 992-995 CC denotes and
T1251 996-1001 NNS denotes women
T1252 1002-1004 VBZ denotes is
T1246 1005-1015 VBN denotes associated
T1253 1016-1020 IN denotes with
T1254 1021-1022 DT denotes a
T1255 1023-1032 NN denotes reduction
T1256 1033-1035 IN denotes in
T1257 1036-1040 NN denotes bone
T1258 1041-1045 NN denotes mass
T1259 1046-1049 CC denotes and
T1260 1050-1052 DT denotes an
T1261 1053-1061 NN denotes increase
T1262 1062-1064 IN denotes in
T1263 1065-1076 VBG denotes circulating
T1264 1077-1083 NNS denotes levels
T1265 1084-1086 IN denotes of
T1266 1087-1091 NN denotes bone
T1267 1092-1102 NNS denotes biomarkers
T1268 1103-1104 -LRB- denotes [
T1269 1104-1106 CD denotes 15
T1270 1106-1107 -RRB- denotes ]
T1271 1107-1108 . denotes .
T1272 1108-1397 sentence denotes These changes have been attributed primarily to nutritional deficits resulting in alterations in the parathyroid hormone–vitamin D axis [16], to gonadal hormone deficiency [17], to leptin levels and the sympathetic nervous system [8,18–20], and to alterations in bone cell apoptosis [21].
T1273 1109-1114 DT denotes These
T1274 1115-1122 NNS denotes changes
T1276 1123-1127 VBP denotes have
T1277 1128-1132 VBN denotes been
T1275 1133-1143 VBN denotes attributed
T1278 1144-1153 RB denotes primarily
T1279 1154-1156 IN denotes to
T1280 1157-1168 JJ denotes nutritional
T1281 1169-1177 NNS denotes deficits
T1282 1178-1187 VBG denotes resulting
T1283 1188-1190 IN denotes in
T1284 1191-1202 NNS denotes alterations
T1285 1203-1205 IN denotes in
T1286 1206-1209 DT denotes the
T1288 1210-1221 NN denotes parathyroid
T1289 1222-1229 NN denotes hormone
T1290 1229-1230 HYPH denotes –
T1291 1230-1237 NN denotes vitamin
T1292 1238-1239 NN denotes D
T1287 1240-1244 NN denotes axis
T1293 1245-1246 -LRB- denotes [
T1294 1246-1248 CD denotes 16
T1295 1248-1249 -RRB- denotes ]
T1296 1249-1251 , denotes ,
T1297 1251-1253 IN denotes to
T1298 1254-1261 JJ denotes gonadal
T1300 1262-1269 NN denotes hormone
T1299 1270-1280 NN denotes deficiency
T1301 1281-1282 -LRB- denotes [
T1302 1282-1284 CD denotes 17
T1303 1284-1285 -RRB- denotes ]
T1304 1285-1287 , denotes ,
T1305 1287-1289 IN denotes to
T1306 1290-1296 NN denotes leptin
T1307 1297-1303 NNS denotes levels
T1308 1304-1307 CC denotes and
T1309 1308-1311 DT denotes the
T1311 1312-1323 JJ denotes sympathetic
T1312 1324-1331 JJ denotes nervous
T1310 1332-1338 NN denotes system
T1313 1339-1340 -LRB- denotes [
T1314 1340-1341 CD denotes 8
T1315 1341-1342 , denotes ,
T1316 1342-1344 CD denotes 18
T1317 1344-1345 SYM denotes –
T1318 1345-1347 CD denotes 20
T1319 1347-1348 -RRB- denotes ]
T1320 1348-1350 , denotes ,
T1321 1350-1353 CC denotes and
T1322 1354-1356 IN denotes to
T1323 1357-1368 NNS denotes alterations
T1324 1369-1371 IN denotes in
T1325 1372-1376 NN denotes bone
T1326 1377-1381 NN denotes cell
T1327 1382-1391 NN denotes apoptosis
T1328 1392-1393 -LRB- denotes [
T1329 1393-1395 CD denotes 21
T1330 1395-1396 -RRB- denotes ]
T1331 1396-1397 . denotes .
T1332 1397-1629 sentence denotes Bone loss in the elderly has also been attributed to alterations in the response of bone marrow stromal cells to their microenvironment that favors differentiation down the adipocyte lineage rather than the osteoblast lineage [22].
T1333 1398-1402 NN denotes Bone
T1334 1403-1407 NN denotes loss
T1336 1408-1410 IN denotes in
T1337 1411-1414 DT denotes the
T1338 1415-1422 JJ denotes elderly
T1339 1423-1426 VBZ denotes has
T1340 1427-1431 RB denotes also
T1341 1432-1436 VBN denotes been
T1335 1437-1447 VBN denotes attributed
T1342 1448-1450 IN denotes to
T1343 1451-1462 NNS denotes alterations
T1344 1463-1465 IN denotes in
T1345 1466-1469 DT denotes the
T1346 1470-1478 NN denotes response
T1347 1479-1481 IN denotes of
T1348 1482-1486 NN denotes bone
T1349 1487-1493 NN denotes marrow
T1351 1494-1501 JJ denotes stromal
T1350 1502-1507 NNS denotes cells
T1352 1508-1510 IN denotes to
T1353 1511-1516 PRP$ denotes their
T1354 1517-1533 NN denotes microenvironment
T1355 1534-1538 WDT denotes that
T1356 1539-1545 VBZ denotes favors
T1357 1546-1561 NN denotes differentiation
T1358 1562-1566 IN denotes down
T1359 1567-1570 DT denotes the
T1361 1571-1580 NN denotes adipocyte
T1360 1581-1588 NN denotes lineage
T1362 1589-1595 RB denotes rather
T1363 1596-1600 IN denotes than
T1364 1601-1604 DT denotes the
T1366 1605-1615 NN denotes osteoblast
T1365 1616-1623 NN denotes lineage
T1367 1624-1625 -LRB- denotes [
T1368 1625-1627 CD denotes 22
T1369 1627-1628 -RRB- denotes ]
T1370 1628-1629 . denotes .
T1371 1629-1761 sentence denotes Aging has long been associated with an increase in marrow fat, where the generation of adipocytes is favored over osteoblasts [23].
T1372 1630-1635 NN denotes Aging
T1374 1636-1639 VBZ denotes has
T1375 1640-1644 RB denotes long
T1376 1645-1649 VBN denotes been
T1373 1650-1660 VBN denotes associated
T1377 1661-1665 IN denotes with
T1378 1666-1668 DT denotes an
T1379 1669-1677 NN denotes increase
T1380 1678-1680 IN denotes in
T1381 1681-1687 NN denotes marrow
T1382 1688-1691 NN denotes fat
T1383 1691-1693 , denotes ,
T1384 1693-1698 WRB denotes where
T1386 1699-1702 DT denotes the
T1387 1703-1713 NN denotes generation
T1388 1714-1716 IN denotes of
T1389 1717-1727 NNS denotes adipocytes
T1390 1728-1730 VBZ denotes is
T1385 1731-1738 VBN denotes favored
T1391 1739-1743 IN denotes over
T1392 1744-1755 NNS denotes osteoblasts
T1393 1756-1757 -LRB- denotes [
T1394 1757-1759 CD denotes 23
T1395 1759-1760 -RRB- denotes ]
T1396 1760-1761 . denotes .
T1397 1761-1981 sentence denotes Osteoblasts and adipocytes are derived from a common mesenchymal precursor cell present in bone marrow, and the factors that control this age-induced switch toward adipogenic differentiation is not well understood [24].
T1398 1762-1773 NNS denotes Osteoblasts
T1400 1774-1777 CC denotes and
T1401 1778-1788 NNS denotes adipocytes
T1402 1789-1792 VBP denotes are
T1399 1793-1800 VBN denotes derived
T1403 1801-1805 IN denotes from
T1404 1806-1807 DT denotes a
T1406 1808-1814 JJ denotes common
T1407 1815-1826 JJ denotes mesenchymal
T1408 1827-1836 NN denotes precursor
T1405 1837-1841 NN denotes cell
T1409 1842-1849 JJ denotes present
T1410 1850-1852 IN denotes in
T1411 1853-1857 NN denotes bone
T1412 1858-1864 NN denotes marrow
T1413 1864-1866 , denotes ,
T1414 1866-1869 CC denotes and
T1415 1870-1873 DT denotes the
T1416 1874-1881 NNS denotes factors
T1418 1882-1886 WDT denotes that
T1419 1887-1894 VBP denotes control
T1420 1895-1899 DT denotes this
T1422 1900-1903 NN denotes age
T1424 1903-1904 HYPH denotes -
T1423 1904-1911 VBN denotes induced
T1421 1912-1918 NN denotes switch
T1425 1919-1925 IN denotes toward
T1426 1926-1936 JJ denotes adipogenic
T1427 1937-1952 NN denotes differentiation
T1428 1953-1955 VBZ denotes is
T1429 1956-1959 RB denotes not
T1430 1960-1964 RB denotes well
T1417 1965-1975 VBN denotes understood
T1431 1976-1977 -LRB- denotes [
T1432 1977-1979 CD denotes 24
T1433 1979-1980 -RRB- denotes ]
T1434 1980-1981 . denotes .
T1435 1981-2262 sentence denotes While several transcriptional regulatory proteins have been associated with cell fate determination of bone marrow mesenchymal cells, including peroxisome proliferator–activated receptor γ (PPARγ) and KLF5 [25–30], the role of RNA binding proteins in this process remains unknown.
T1436 1982-1987 IN denotes While
T1438 1988-1995 JJ denotes several
T1440 1996-2011 JJ denotes transcriptional
T1441 2012-2022 JJ denotes regulatory
T1439 2023-2031 NN denotes proteins
T1442 2032-2036 VBP denotes have
T1443 2037-2041 VBN denotes been
T1437 2042-2052 VBN denotes associated
T1445 2053-2057 IN denotes with
T1446 2058-2062 NN denotes cell
T1448 2063-2067 NN denotes fate
T1447 2068-2081 NN denotes determination
T1449 2082-2084 IN denotes of
T1450 2085-2089 NN denotes bone
T1451 2090-2096 NN denotes marrow
T1453 2097-2108 JJ denotes mesenchymal
T1452 2109-2114 NNS denotes cells
T1454 2114-2116 , denotes ,
T1455 2116-2125 VBG denotes including
T1456 2126-2136 NN denotes peroxisome
T1458 2137-2149 NN denotes proliferator
T1460 2149-2150 HYPH denotes –
T1459 2150-2159 VBN denotes activated
T1457 2160-2168 NN denotes receptor
T1461 2169-2170 SYM denotes γ
T1462 2171-2172 -LRB- denotes (
T1463 2172-2177 NN denotes PPARγ
T1464 2177-2178 -RRB- denotes )
T1465 2179-2182 CC denotes and
T1466 2183-2187 NN denotes KLF5
T1467 2188-2189 -LRB- denotes [
T1468 2189-2191 CD denotes 25
T1469 2191-2192 SYM denotes –
T1470 2192-2194 CD denotes 30
T1471 2194-2195 -RRB- denotes ]
T1472 2195-2197 , denotes ,
T1473 2197-2200 DT denotes the
T1474 2201-2205 NN denotes role
T1475 2206-2208 IN denotes of
T1476 2209-2212 NN denotes RNA
T1478 2213-2220 NN denotes binding
T1477 2221-2229 NN denotes proteins
T1479 2230-2232 IN denotes in
T1480 2233-2237 DT denotes this
T1481 2238-2245 NN denotes process
T1444 2246-2253 VBZ denotes remains
T1482 2254-2261 JJ denotes unknown
T1483 2261-2262 . denotes .
T1484 2262-2348 sentence denotes RNA binding proteins of the KH type are known regulators of cellular differentiation.
T1485 2263-2266 NN denotes RNA
T1487 2267-2274 NN denotes binding
T1486 2275-2283 NN denotes proteins
T1489 2284-2286 IN denotes of
T1490 2287-2290 DT denotes the
T1492 2291-2293 NN denotes KH
T1491 2294-2298 NN denotes type
T1488 2299-2302 VBP denotes are
T1493 2303-2308 JJ denotes known
T1494 2309-2319 NNS denotes regulators
T1495 2320-2322 IN denotes of
T1496 2323-2331 JJ denotes cellular
T1497 2332-2347 NN denotes differentiation
T1498 2347-2348 . denotes .
T1499 2348-2538 sentence denotes For example, expression defects in the KH domain proteins NOVA and FMRP are known to cause paraneoplastic neurologic disorders [31] and the fragile X syndrome, respectively, in humans [32].
T1500 2349-2352 IN denotes For
T1502 2353-2360 NN denotes example
T1503 2360-2362 , denotes ,
T1504 2362-2372 NN denotes expression
T1505 2373-2380 NNS denotes defects
T1506 2381-2383 IN denotes in
T1507 2384-2387 DT denotes the
T1509 2388-2390 NN denotes KH
T1510 2391-2397 NN denotes domain
T1508 2398-2406 NN denotes proteins
T1511 2407-2411 NN denotes NOVA
T1512 2412-2415 CC denotes and
T1513 2416-2420 NN denotes FMRP
T1514 2421-2424 VBP denotes are
T1501 2425-2430 VBN denotes known
T1515 2431-2433 TO denotes to
T1516 2434-2439 VB denotes cause
T1517 2440-2454 JJ denotes paraneoplastic
T1519 2455-2465 JJ denotes neurologic
T1518 2466-2475 NNS denotes disorders
T1520 2476-2477 -LRB- denotes [
T1521 2477-2479 CD denotes 31
T1522 2479-2480 -RRB- denotes ]
T1523 2481-2484 CC denotes and
T1524 2485-2488 DT denotes the
T1526 2489-2496 JJ denotes fragile
T1527 2497-2498 NN denotes X
T1525 2499-2507 NN denotes syndrome
T1528 2507-2509 , denotes ,
T1529 2509-2521 RB denotes respectively
T1530 2521-2523 , denotes ,
T1531 2523-2525 IN denotes in
T1532 2526-2532 NNS denotes humans
T1533 2533-2534 -LRB- denotes [
T1534 2534-2536 CD denotes 32
T1535 2536-2537 -RRB- denotes ]
T1536 2537-2538 . denotes .
T1537 2538-2673 sentence denotes The phenotype of the quaking viable mice suggests a role for the QUAKING RNA binding protein in oligodendrocytes and myelination [33].
T1538 2539-2542 DT denotes The
T1539 2543-2552 NN denotes phenotype
T1541 2553-2555 IN denotes of
T1542 2556-2559 DT denotes the
T1544 2560-2567 NN denotes quaking
T1545 2568-2574 JJ denotes viable
T1543 2575-2579 NNS denotes mice
T1540 2580-2588 VBZ denotes suggests
T1546 2589-2590 DT denotes a
T1547 2591-2595 NN denotes role
T1548 2596-2599 IN denotes for
T1549 2600-2603 DT denotes the
T1551 2604-2611 NN denotes QUAKING
T1552 2612-2615 NN denotes RNA
T1553 2616-2623 NN denotes binding
T1550 2624-2631 NN denotes protein
T1554 2632-2634 IN denotes in
T1555 2635-2651 NNS denotes oligodendrocytes
T1556 2652-2655 CC denotes and
T1557 2656-2667 NN denotes myelination
T1558 2668-2669 -LRB- denotes [
T1559 2669-2671 CD denotes 33
T1560 2671-2672 -RRB- denotes ]
T1561 2672-2673 . denotes .
T1562 2673-2872 sentence denotes Indeed, the ectopic expression of the QKI-6/7 isoforms in vivo led to the formation of glial cells rather than neurons from neural progenitor, demonstrating its role in cell fate determination [34].
T1563 2674-2680 RB denotes Indeed
T1565 2680-2682 , denotes ,
T1566 2682-2685 DT denotes the
T1568 2686-2693 JJ denotes ectopic
T1567 2694-2704 NN denotes expression
T1569 2705-2707 IN denotes of
T1570 2708-2711 DT denotes the
T1572 2712-2715 NN denotes QKI
T1574 2715-2716 HYPH denotes -
T1575 2716-2717 CD denotes 6
T1576 2717-2718 HYPH denotes /
T1573 2718-2719 CD denotes 7
T1571 2720-2728 NNS denotes isoforms
T1577 2729-2731 FW denotes in
T1578 2732-2736 FW denotes vivo
T1564 2737-2740 VBD denotes led
T1579 2741-2743 IN denotes to
T1580 2744-2747 DT denotes the
T1581 2748-2757 NN denotes formation
T1582 2758-2760 IN denotes of
T1583 2761-2766 JJ denotes glial
T1584 2767-2772 NNS denotes cells
T1585 2773-2779 RB denotes rather
T1586 2780-2784 IN denotes than
T1587 2785-2792 NNS denotes neurons
T1588 2793-2797 IN denotes from
T1589 2798-2804 JJ denotes neural
T1590 2805-2815 NN denotes progenitor
T1591 2815-2817 , denotes ,
T1592 2817-2830 VBG denotes demonstrating
T1593 2831-2834 PRP$ denotes its
T1594 2835-2839 NN denotes role
T1595 2840-2842 IN denotes in
T1596 2843-2847 NN denotes cell
T1598 2848-2852 NN denotes fate
T1597 2853-2866 NN denotes determination
T1599 2867-2868 -LRB- denotes [
T1600 2868-2870 CD denotes 34
T1601 2870-2871 -RRB- denotes ]
T1602 2871-2872 . denotes .
T1603 2872-3109 sentence denotes The loss of GLD-1 protein in Caenorhabditis elegans prevents the appearance of stem cells [35], and the absence of the KH domain protein HOW in Drosophila prevents muscle differentiation and results in flies with held-out-wings [36,37].
T1604 2873-2876 DT denotes The
T1605 2877-2881 NN denotes loss
T1607 2882-2884 IN denotes of
T1608 2885-2888 NN denotes GLD
T1610 2888-2889 HYPH denotes -
T1611 2889-2890 CD denotes 1
T1609 2891-2898 NN denotes protein
T1612 2899-2901 IN denotes in
T1613 2902-2916 NNP denotes Caenorhabditis
T1614 2917-2924 NNP denotes elegans
T1606 2925-2933 VBZ denotes prevents
T1615 2934-2937 DT denotes the
T1616 2938-2948 NN denotes appearance
T1617 2949-2951 IN denotes of
T1618 2952-2956 NN denotes stem
T1619 2957-2962 NNS denotes cells
T1620 2963-2964 -LRB- denotes [
T1621 2964-2966 CD denotes 35
T1622 2966-2967 -RRB- denotes ]
T1623 2967-2969 , denotes ,
T1624 2969-2972 CC denotes and
T1625 2973-2976 DT denotes the
T1626 2977-2984 NN denotes absence
T1628 2985-2987 IN denotes of
T1629 2988-2991 DT denotes the
T1631 2992-2994 NN denotes KH
T1632 2995-3001 NN denotes domain
T1630 3002-3009 NN denotes protein
T1633 3010-3013 NN denotes HOW
T1634 3014-3016 IN denotes in
T1635 3017-3027 NNP denotes Drosophila
T1627 3028-3036 VBZ denotes prevents
T1636 3037-3043 NN denotes muscle
T1637 3044-3059 NN denotes differentiation
T1638 3060-3063 CC denotes and
T1639 3064-3071 VBZ denotes results
T1640 3072-3074 IN denotes in
T1641 3075-3080 NNS denotes flies
T1642 3081-3085 IN denotes with
T1643 3086-3090 VBN denotes held
T1645 3090-3091 HYPH denotes -
T1646 3091-3094 RP denotes out
T1647 3094-3095 HYPH denotes -
T1644 3095-3100 NNS denotes wings
T1648 3101-3102 -LRB- denotes [
T1650 3102-3104 CD denotes 36
T1651 3104-3105 , denotes ,
T1649 3105-3107 CD denotes 37
T1652 3107-3108 -RRB- denotes ]
T1653 3108-3109 . denotes .
T1654 3109-3292 sentence denotes The Src substrate associated in mitosis of 68 kDa (Sam68) is also a member of the family of KH domain RNA binding proteins [38]; however, its physiologic role has remained undefined.
T1655 3110-3113 DT denotes The
T1657 3114-3117 NN denotes Src
T1656 3118-3127 NN denotes substrate
T1659 3128-3138 VBN denotes associated
T1660 3139-3141 IN denotes in
T1661 3142-3149 NN denotes mitosis
T1662 3150-3152 IN denotes of
T1663 3153-3155 CD denotes 68
T1664 3156-3159 NN denotes kDa
T1665 3160-3161 -LRB- denotes (
T1666 3161-3166 NN denotes Sam68
T1667 3166-3167 -RRB- denotes )
T1658 3168-3170 VBZ denotes is
T1669 3171-3175 RB denotes also
T1670 3176-3177 DT denotes a
T1671 3178-3184 NN denotes member
T1672 3185-3187 IN denotes of
T1673 3188-3191 DT denotes the
T1674 3192-3198 NN denotes family
T1675 3199-3201 IN denotes of
T1676 3202-3204 NN denotes KH
T1678 3205-3211 NN denotes domain
T1679 3212-3215 NN denotes RNA
T1680 3216-3223 NN denotes binding
T1677 3224-3232 NN denotes proteins
T1681 3233-3234 -LRB- denotes [
T1682 3234-3236 CD denotes 38
T1683 3236-3237 -RRB- denotes ]
T1684 3237-3238 : denotes ;
T1685 3239-3246 RB denotes however
T1686 3246-3248 , denotes ,
T1687 3248-3251 PRP$ denotes its
T1689 3252-3263 JJ denotes physiologic
T1688 3264-3268 NN denotes role
T1690 3269-3272 VBZ denotes has
T1668 3273-3281 VBN denotes remained
T1691 3282-3291 JJ denotes undefined
T1692 3291-3292 . denotes .
T1693 3292-3471 sentence denotes Sam68 was identified as an SH3 and SH2 domain interacting protein for Src family kinases and is also a known substrate of Src kinases [39–42] and of the breast tumor kinase [43].
T1694 3293-3298 NN denotes Sam68
T1696 3299-3302 VBD denotes was
T1695 3303-3313 VBN denotes identified
T1697 3314-3316 IN denotes as
T1698 3317-3319 DT denotes an
T1700 3320-3323 NN denotes SH3
T1701 3324-3327 CC denotes and
T1702 3328-3331 NN denotes SH2
T1703 3332-3338 NN denotes domain
T1704 3339-3350 VBG denotes interacting
T1699 3351-3358 NN denotes protein
T1705 3359-3362 IN denotes for
T1706 3363-3366 NN denotes Src
T1708 3367-3373 NN denotes family
T1707 3374-3381 NNS denotes kinases
T1709 3382-3385 CC denotes and
T1710 3386-3388 VBZ denotes is
T1711 3389-3393 RB denotes also
T1712 3394-3395 DT denotes a
T1714 3396-3401 JJ denotes known
T1713 3402-3411 NN denotes substrate
T1715 3412-3414 IN denotes of
T1716 3415-3418 NN denotes Src
T1717 3419-3426 NNS denotes kinases
T1718 3427-3428 -LRB- denotes [
T1719 3428-3430 CD denotes 39
T1720 3430-3431 SYM denotes –
T1721 3431-3433 CD denotes 42
T1722 3433-3434 -RRB- denotes ]
T1723 3435-3438 CC denotes and
T1724 3439-3441 IN denotes of
T1725 3442-3445 DT denotes the
T1727 3446-3452 NN denotes breast
T1728 3453-3458 NN denotes tumor
T1726 3459-3465 NN denotes kinase
T1729 3466-3467 -LRB- denotes [
T1730 3467-3469 CD denotes 43
T1731 3469-3470 -RRB- denotes ]
T1732 3470-3471 . denotes .
T1733 3471-3585 sentence denotes Sam68 has been shown to facilitate the export of unspliced HIV RNA [44] and to regulate pre-mRNA processing [45].
T1734 3472-3477 NN denotes Sam68
T1736 3478-3481 VBZ denotes has
T1737 3482-3486 VBN denotes been
T1735 3487-3492 VBN denotes shown
T1738 3493-3495 TO denotes to
T1739 3496-3506 VB denotes facilitate
T1740 3507-3510 DT denotes the
T1741 3511-3517 NN denotes export
T1742 3518-3520 IN denotes of
T1743 3521-3530 JJ denotes unspliced
T1745 3531-3534 NN denotes HIV
T1744 3535-3538 NN denotes RNA
T1746 3539-3540 -LRB- denotes [
T1747 3540-3542 CD denotes 44
T1748 3542-3543 -RRB- denotes ]
T1749 3544-3547 CC denotes and
T1750 3548-3550 TO denotes to
T1751 3551-3559 VB denotes regulate
T1752 3560-3568 NN denotes pre-mRNA
T1753 3569-3579 NN denotes processing
T1754 3580-3581 -LRB- denotes [
T1755 3581-3583 CD denotes 45
T1756 3583-3584 -RRB- denotes ]
T1757 3584-3585 . denotes .
T1758 3585-3691 sentence denotes In the present paper, we report the generation of Sam68−/− mice and analysis of their skeletal phenotype.
T1759 3586-3588 IN denotes In
T1761 3589-3592 DT denotes the
T1763 3593-3600 JJ denotes present
T1762 3601-3606 NN denotes paper
T1764 3606-3608 , denotes ,
T1765 3608-3610 PRP denotes we
T1760 3611-3617 VBP denotes report
T1766 3618-3621 DT denotes the
T1767 3622-3632 NN denotes generation
T1768 3633-3635 IN denotes of
T1769 3636-3641 NN denotes Sam68
T1771 3641-3642 SYM denotes −
T1772 3642-3643 HYPH denotes /
T1773 3643-3644 SYM denotes −
T1770 3645-3649 NNS denotes mice
T1774 3650-3653 CC denotes and
T1775 3654-3662 NN denotes analysis
T1776 3663-3665 IN denotes of
T1777 3666-3671 PRP$ denotes their
T1779 3672-3680 JJ denotes skeletal
T1778 3681-3690 NN denotes phenotype
T1780 3690-3691 . denotes .
T1781 3691-3865 sentence denotes Our data indicate that the absence of Sam68 confers resistance to age-related bone loss in mice such that old Sam68 have a higher bone mass than their wild-type littermates.
T1782 3692-3695 PRP$ denotes Our
T1783 3696-3700 NNS denotes data
T1784 3701-3709 VBP denotes indicate
T1785 3710-3714 IN denotes that
T1787 3715-3718 DT denotes the
T1788 3719-3726 NN denotes absence
T1789 3727-3729 IN denotes of
T1790 3730-3735 NN denotes Sam68
T1786 3736-3743 VBZ denotes confers
T1791 3744-3754 NN denotes resistance
T1792 3755-3757 IN denotes to
T1793 3758-3761 NN denotes age
T1795 3761-3762 HYPH denotes -
T1794 3762-3769 VBN denotes related
T1797 3770-3774 NN denotes bone
T1796 3775-3779 NN denotes loss
T1798 3780-3782 IN denotes in
T1799 3783-3787 NNS denotes mice
T1800 3788-3792 JJ denotes such
T1802 3793-3797 IN denotes that
T1803 3798-3801 JJ denotes old
T1804 3802-3807 NN denotes Sam68
T1801 3808-3812 VBP denotes have
T1805 3813-3814 DT denotes a
T1807 3815-3821 JJR denotes higher
T1808 3822-3826 NN denotes bone
T1806 3827-3831 NN denotes mass
T1809 3832-3836 IN denotes than
T1810 3837-3842 PRP$ denotes their
T1812 3843-3847 JJ denotes wild
T1814 3847-3848 HYPH denotes -
T1813 3848-3852 NN denotes type
T1811 3853-3864 NNS denotes littermates
T1815 3864-3865 . denotes .
T1816 3865-4124 sentence denotes We provide evidence that Sam68 regulates the differentiation of bone marrow stromal cells by showing that cells isolated from Sam68−/− animals had enhanced osteogenic activity and decreased adipogenic activity than those harvested from wild-type littermates.
T1817 3866-3868 PRP denotes We
T1818 3869-3876 VBP denotes provide
T1819 3877-3885 NN denotes evidence
T1820 3886-3890 IN denotes that
T1822 3891-3896 NN denotes Sam68
T1821 3897-3906 VBZ denotes regulates
T1823 3907-3910 DT denotes the
T1824 3911-3926 NN denotes differentiation
T1825 3927-3929 IN denotes of
T1826 3930-3934 NN denotes bone
T1827 3935-3941 NN denotes marrow
T1829 3942-3949 JJ denotes stromal
T1828 3950-3955 NNS denotes cells
T1830 3956-3958 IN denotes by
T1831 3959-3966 VBG denotes showing
T1832 3967-3971 IN denotes that
T1834 3972-3977 NNS denotes cells
T1835 3978-3986 VBN denotes isolated
T1836 3987-3991 IN denotes from
T1837 3992-3997 NN denotes Sam68
T1839 3997-3998 SYM denotes −
T1840 3998-3999 HYPH denotes /
T1841 3999-4000 SYM denotes −
T1838 4001-4008 NNS denotes animals
T1833 4009-4012 VBD denotes had
T1842 4013-4021 VBN denotes enhanced
T1844 4022-4032 JJ denotes osteogenic
T1843 4033-4041 NN denotes activity
T1845 4042-4045 CC denotes and
T1846 4046-4055 VBN denotes decreased
T1848 4056-4066 JJ denotes adipogenic
T1847 4067-4075 NN denotes activity
T1849 4076-4080 IN denotes than
T1850 4081-4086 DT denotes those
T1851 4087-4096 VBN denotes harvested
T1852 4097-4101 IN denotes from
T1853 4102-4106 JJ denotes wild
T1855 4106-4107 HYPH denotes -
T1854 4107-4111 NN denotes type
T1856 4112-4123 NNS denotes littermates
T1857 4123-4124 . denotes .
T1858 4124-4333 sentence denotes Furthermore, Sam68−/− mouse embryo fibroblasts (MEFs) were impaired in their capacity to differentiate into adipocytes, consistent with Sam68 being a regulator of bone marrow mesenchymal cell differentiation.
T1859 4125-4136 RB denotes Furthermore
T1861 4136-4138 , denotes ,
T1862 4138-4143 NN denotes Sam68
T1863 4143-4144 SYM denotes −
T1864 4144-4145 HYPH denotes /
T1865 4145-4146 SYM denotes −
T1866 4147-4152 NN denotes mouse
T1867 4153-4159 NN denotes embryo
T1868 4160-4171 NNS denotes fibroblasts
T1869 4172-4173 -LRB- denotes (
T1870 4173-4177 NNS denotes MEFs
T1871 4177-4178 -RRB- denotes )
T1872 4179-4183 VBD denotes were
T1860 4184-4192 VBN denotes impaired
T1873 4193-4195 IN denotes in
T1874 4196-4201 PRP$ denotes their
T1875 4202-4210 NN denotes capacity
T1876 4211-4213 TO denotes to
T1877 4214-4227 VB denotes differentiate
T1878 4228-4232 IN denotes into
T1879 4233-4243 NNS denotes adipocytes
T1880 4243-4245 , denotes ,
T1881 4245-4255 JJ denotes consistent
T1882 4256-4260 IN denotes with
T1883 4261-4266 NN denotes Sam68
T1884 4267-4272 VBG denotes being
T1885 4273-4274 DT denotes a
T1886 4275-4284 NN denotes regulator
T1887 4285-4287 IN denotes of
T1888 4288-4292 NN denotes bone
T1889 4293-4299 NN denotes marrow
T1891 4300-4311 JJ denotes mesenchymal
T1892 4312-4316 NN denotes cell
T1890 4317-4332 NN denotes differentiation
T1893 4332-4333 . denotes .
T1894 4333-4449 sentence denotes These results also characterize a new animal model to study bone metabolism, regeneration, and repair during aging.
T1895 4334-4339 DT denotes These
T1896 4340-4347 NNS denotes results
T1898 4348-4352 RB denotes also
T1897 4353-4365 VBP denotes characterize
T1899 4366-4367 DT denotes a
T1901 4368-4371 JJ denotes new
T1902 4372-4378 NN denotes animal
T1900 4379-4384 NN denotes model
T1903 4385-4387 TO denotes to
T1904 4388-4393 VB denotes study
T1905 4394-4398 NN denotes bone
T1906 4399-4409 NN denotes metabolism
T1907 4409-4411 , denotes ,
T1908 4411-4423 NN denotes regeneration
T1909 4423-4425 , denotes ,
T1910 4425-4428 CC denotes and
T1911 4429-4435 NN denotes repair
T1912 4436-4442 IN denotes during
T1913 4443-4448 NN denotes aging
T1914 4448-4449 . denotes .
T2061 4460-4465 NN denotes Sam68
T2063 4466-4468 VBZ denotes Is
T2062 4469-4478 VBN denotes Expressed
T2064 4479-4481 IN denotes in
T2065 4482-4485 DT denotes the
T2067 4486-4496 VBG denotes Developing
T2066 4497-4505 NN denotes Skeleton
T2068 4506-4508 IN denotes of
T2069 4509-4518 JJ denotes Embryonic
T2070 4519-4523 NNS denotes Mice
T2071 4523-4649 sentence denotes The Sam68 mRNA is known to be widely expressed [38], whereas its pattern of protein expression in vivo remains to be defined.
T2072 4524-4527 DT denotes The
T2074 4528-4533 NN denotes Sam68
T2073 4534-4538 NN denotes mRNA
T2076 4539-4541 VBZ denotes is
T2075 4542-4547 VBN denotes known
T2077 4548-4550 TO denotes to
T2079 4551-4553 VB denotes be
T2080 4554-4560 RB denotes widely
T2078 4561-4570 VBN denotes expressed
T2081 4571-4572 -LRB- denotes [
T2082 4572-4574 CD denotes 38
T2083 4574-4575 -RRB- denotes ]
T2084 4575-4577 , denotes ,
T2085 4577-4584 IN denotes whereas
T2087 4585-4588 PRP$ denotes its
T2088 4589-4596 NN denotes pattern
T2089 4597-4599 IN denotes of
T2090 4600-4607 NN denotes protein
T2091 4608-4618 NN denotes expression
T2092 4619-4621 FW denotes in
T2093 4622-4626 FW denotes vivo
T2086 4627-4634 VBZ denotes remains
T2094 4635-4637 TO denotes to
T2096 4638-4640 VB denotes be
T2095 4641-4648 VBN denotes defined
T2097 4648-4649 . denotes .
T2098 4649-4916 sentence denotes Sections of paraffin-embedded wild-type E14.5 and E16.5 embryonic mice were immunostained with the well-characterized AD1 anti-Sam68 antibody, raised in rabbits against an immunizing peptide that corresponds to amino acids 330 to 348 of the mouse Sam68 protein [46].
T2099 4650-4658 NNS denotes Sections
T2101 4659-4661 IN denotes of
T2102 4662-4670 NN denotes paraffin
T2104 4670-4671 HYPH denotes -
T2103 4671-4679 VBN denotes embedded
T2106 4680-4684 JJ denotes wild
T2108 4684-4685 HYPH denotes -
T2107 4685-4689 NN denotes type
T2109 4690-4695 NN denotes E14.5
T2110 4696-4699 CC denotes and
T2111 4700-4705 NN denotes E16.5
T2112 4706-4715 JJ denotes embryonic
T2105 4716-4720 NNS denotes mice
T2113 4721-4725 VBD denotes were
T2100 4726-4739 VBN denotes immunostained
T2114 4740-4744 IN denotes with
T2115 4745-4748 DT denotes the
T2117 4749-4753 RB denotes well
T2119 4753-4754 HYPH denotes -
T2118 4754-4767 VBN denotes characterized
T2120 4768-4771 NN denotes AD1
T2121 4772-4782 JJ denotes anti-Sam68
T2116 4783-4791 NN denotes antibody
T2122 4791-4793 , denotes ,
T2123 4793-4799 VBN denotes raised
T2124 4800-4802 IN denotes in
T2125 4803-4810 NNS denotes rabbits
T2126 4811-4818 IN denotes against
T2127 4819-4821 DT denotes an
T2129 4822-4832 VBG denotes immunizing
T2128 4833-4840 NN denotes peptide
T2130 4841-4845 WDT denotes that
T2131 4846-4857 VBZ denotes corresponds
T2132 4858-4860 IN denotes to
T2133 4861-4866 NN denotes amino
T2135 4867-4872 NNS denotes acids
T2134 4873-4876 CD denotes 330
T2136 4877-4879 IN denotes to
T2137 4880-4883 CD denotes 348
T2138 4884-4886 IN denotes of
T2139 4887-4890 DT denotes the
T2141 4891-4896 NN denotes mouse
T2142 4897-4902 NN denotes Sam68
T2140 4903-4910 NN denotes protein
T2143 4911-4912 -LRB- denotes [
T2144 4912-4914 CD denotes 46
T2145 4914-4915 -RRB- denotes ]
T2146 4915-4916 . denotes .
T2147 4916-5045 sentence denotes Sam68 immunoreactivity was observed in the skeleton and soft tissues of developing wild-type mice at E14.5 and E16.5 (Figure 1).
T2148 4917-4922 NN denotes Sam68
T2149 4923-4939 NN denotes immunoreactivity
T2151 4940-4943 VBD denotes was
T2150 4944-4952 VBN denotes observed
T2152 4953-4955 IN denotes in
T2153 4956-4959 DT denotes the
T2154 4960-4968 NN denotes skeleton
T2155 4969-4972 CC denotes and
T2156 4973-4977 JJ denotes soft
T2157 4978-4985 NNS denotes tissues
T2158 4986-4988 IN denotes of
T2159 4989-4999 VBG denotes developing
T2161 5000-5004 JJ denotes wild
T2163 5004-5005 HYPH denotes -
T2162 5005-5009 NN denotes type
T2160 5010-5014 NNS denotes mice
T2164 5015-5017 IN denotes at
T2165 5018-5023 NN denotes E14.5
T2166 5024-5027 CC denotes and
T2167 5028-5033 NN denotes E16.5
T2168 5034-5035 -LRB- denotes (
T2169 5035-5041 NN denotes Figure
T2170 5042-5043 CD denotes 1
T2171 5043-5044 -RRB- denotes )
T2172 5044-5045 . denotes .
T2173 5045-5417 sentence denotes Intense staining was seen in the nucleus of cells in the developing brain, heart, and small intestine (Figure 1B), as well as in chondrocytes in the nasal septum and the glandular tissue adjacent to the nasal cartilage (Figure 1C, panels A–D), in the vertebra and intervertebral discs (panels E–H) and in the epiphysis (panels I–K) and metaphysis (panel L) of long bones.
T2174 5046-5053 JJ denotes Intense
T2175 5054-5062 NN denotes staining
T2177 5063-5066 VBD denotes was
T2176 5067-5071 VBN denotes seen
T2178 5072-5074 IN denotes in
T2179 5075-5078 DT denotes the
T2180 5079-5086 NN denotes nucleus
T2181 5087-5089 IN denotes of
T2182 5090-5095 NNS denotes cells
T2183 5096-5098 IN denotes in
T2184 5099-5102 DT denotes the
T2186 5103-5113 VBG denotes developing
T2185 5114-5119 NN denotes brain
T2187 5119-5121 , denotes ,
T2188 5121-5126 NN denotes heart
T2189 5126-5128 , denotes ,
T2190 5128-5131 CC denotes and
T2191 5132-5137 JJ denotes small
T2192 5138-5147 NN denotes intestine
T2193 5148-5149 -LRB- denotes (
T2195 5149-5155 NN denotes Figure
T2194 5156-5158 NN denotes 1B
T2196 5158-5159 -RRB- denotes )
T2197 5159-5161 , denotes ,
T2198 5161-5163 RB denotes as
T2200 5164-5168 RB denotes well
T2199 5169-5171 IN denotes as
T2201 5172-5174 IN denotes in
T2202 5175-5187 NNS denotes chondrocytes
T2203 5188-5190 IN denotes in
T2204 5191-5194 DT denotes the
T2206 5195-5200 JJ denotes nasal
T2205 5201-5207 NN denotes septum
T2207 5208-5211 CC denotes and
T2208 5212-5215 DT denotes the
T2210 5216-5225 JJ denotes glandular
T2209 5226-5232 NN denotes tissue
T2211 5233-5241 JJ denotes adjacent
T2212 5242-5244 IN denotes to
T2213 5245-5248 DT denotes the
T2215 5249-5254 JJ denotes nasal
T2214 5255-5264 NN denotes cartilage
T2216 5265-5266 -LRB- denotes (
T2218 5266-5272 NN denotes Figure
T2217 5273-5275 NN denotes 1C
T2219 5275-5277 , denotes ,
T2220 5277-5283 NNS denotes panels
T2221 5284-5285 NN denotes A
T2222 5285-5286 SYM denotes –
T2223 5286-5287 NN denotes D
T2224 5287-5288 -RRB- denotes )
T2225 5288-5290 , denotes ,
T2226 5290-5292 IN denotes in
T2227 5293-5296 DT denotes the
T2228 5297-5305 NNS denotes vertebra
T2229 5306-5309 CC denotes and
T2230 5310-5324 JJ denotes intervertebral
T2231 5325-5330 NNS denotes discs
T2232 5331-5332 -LRB- denotes (
T2234 5332-5338 NNS denotes panels
T2233 5339-5340 NN denotes E
T2235 5340-5341 SYM denotes –
T2236 5341-5342 NN denotes H
T2237 5342-5343 -RRB- denotes )
T2238 5344-5347 CC denotes and
T2239 5348-5350 IN denotes in
T2240 5351-5354 DT denotes the
T2241 5355-5364 NN denotes epiphysis
T2242 5365-5366 -LRB- denotes (
T2244 5366-5372 NNS denotes panels
T2243 5373-5374 NN denotes I
T2245 5374-5375 SYM denotes –
T2246 5375-5376 NN denotes K
T2247 5376-5377 -RRB- denotes )
T2248 5378-5381 CC denotes and
T2249 5382-5392 NN denotes metaphysis
T2250 5393-5394 -LRB- denotes (
T2252 5394-5399 NN denotes panel
T2251 5400-5401 NN denotes L
T2253 5401-5402 -RRB- denotes )
T2254 5403-5405 IN denotes of
T2255 5406-5410 JJ denotes long
T2256 5411-5416 NNS denotes bones
T2257 5416-5417 . denotes .
T2258 5417-5600 sentence denotes Nuclear staining was observed in proliferating chondrocytes and hypertrophic chondrocytes (Figure 1C, panel H), in osteoblasts (panel L; Dataset S1), and in osteoclasts (Dataset S1).
T2259 5418-5425 JJ denotes Nuclear
T2260 5426-5434 NN denotes staining
T2262 5435-5438 VBD denotes was
T2261 5439-5447 VBN denotes observed
T2263 5448-5450 IN denotes in
T2264 5451-5464 VBG denotes proliferating
T2265 5465-5477 NNS denotes chondrocytes
T2266 5478-5481 CC denotes and
T2267 5482-5494 JJ denotes hypertrophic
T2268 5495-5507 NNS denotes chondrocytes
T2269 5508-5509 -LRB- denotes (
T2271 5509-5515 NN denotes Figure
T2270 5516-5518 NN denotes 1C
T2272 5518-5520 , denotes ,
T2273 5520-5525 NN denotes panel
T2274 5526-5527 NN denotes H
T2275 5527-5528 -RRB- denotes )
T2276 5528-5530 , denotes ,
T2277 5530-5532 IN denotes in
T2278 5533-5544 NNS denotes osteoblasts
T2279 5545-5546 -LRB- denotes (
T2281 5546-5551 NN denotes panel
T2280 5552-5553 NN denotes L
T2282 5553-5554 : denotes ;
T2283 5555-5562 NN denotes Dataset
T2284 5563-5565 NN denotes S1
T2285 5565-5566 -RRB- denotes )
T2286 5566-5568 , denotes ,
T2287 5568-5571 CC denotes and
T2288 5572-5574 IN denotes in
T2289 5575-5586 NNS denotes osteoclasts
T2290 5587-5588 -LRB- denotes (
T2292 5588-5595 NN denotes Dataset
T2291 5596-5598 NN denotes S1
T2293 5598-5599 -RRB- denotes )
T2294 5599-5600 . denotes .
T2295 5600-5745 sentence denotes No staining was observed with preimmune serum or when the antiserum was preadsorbed with the immunizing peptide (Figure 1C, panels B, F, and J).
T2296 5601-5603 DT denotes No
T2297 5604-5612 NN denotes staining
T2299 5613-5616 VBD denotes was
T2298 5617-5625 VBN denotes observed
T2300 5626-5630 IN denotes with
T2301 5631-5640 JJ denotes preimmune
T2302 5641-5646 NN denotes serum
T2303 5647-5649 CC denotes or
T2304 5650-5654 WRB denotes when
T2306 5655-5658 DT denotes the
T2307 5659-5668 NN denotes antiserum
T2308 5669-5672 VBD denotes was
T2305 5673-5684 VBN denotes preadsorbed
T2309 5685-5689 IN denotes with
T2310 5690-5693 DT denotes the
T2312 5694-5704 VBG denotes immunizing
T2311 5705-5712 NN denotes peptide
T2313 5713-5714 -LRB- denotes (
T2315 5714-5720 NN denotes Figure
T2314 5721-5723 NN denotes 1C
T2316 5723-5725 , denotes ,
T2317 5725-5731 NNS denotes panels
T2318 5732-5733 NN denotes B
T2319 5733-5735 , denotes ,
T2320 5735-5736 NN denotes F
T2321 5736-5738 , denotes ,
T2322 5738-5741 CC denotes and
T2323 5742-5743 NN denotes J
T2324 5743-5744 -RRB- denotes )
T2325 5744-5745 . denotes .
T2326 5745-5921 sentence denotes Taken together, these data demonstrate that Sam68 protein is selectively expressed in the developing mouse embryo, with particularly elevated expression in cartilage and bone.
T2327 5746-5751 VBN denotes Taken
T2329 5752-5760 RB denotes together
T2330 5760-5762 , denotes ,
T2331 5762-5767 DT denotes these
T2332 5768-5772 NNS denotes data
T2328 5773-5784 VBP denotes demonstrate
T2333 5785-5789 IN denotes that
T2335 5790-5795 NN denotes Sam68
T2336 5796-5803 NN denotes protein
T2337 5804-5806 VBZ denotes is
T2338 5807-5818 RB denotes selectively
T2334 5819-5828 VBN denotes expressed
T2339 5829-5831 IN denotes in
T2340 5832-5835 DT denotes the
T2342 5836-5846 VBG denotes developing
T2343 5847-5852 NN denotes mouse
T2341 5853-5859 NN denotes embryo
T2344 5859-5861 , denotes ,
T2345 5861-5865 IN denotes with
T2346 5866-5878 RB denotes particularly
T2347 5879-5887 VBN denotes elevated
T2348 5888-5898 NN denotes expression
T2349 5899-5901 IN denotes in
T2350 5902-5911 NN denotes cartilage
T2351 5912-5915 CC denotes and
T2352 5916-5920 NN denotes bone
T2353 5920-5921 . denotes .
T2520 7122-7127 NN denotes Sam68
T2521 7128-7130 VBZ denotes is
T2522 7131-7134 RB denotes Not
T2523 7135-7144 JJ denotes Essential
T2524 7145-7148 IN denotes for
T2525 7149-7154 NN denotes Mouse
T2526 7155-7166 NN denotes Development
T2527 7166-7382 sentence denotes To define the physiologic role of Sam68, we generated Sam68-deficient mice by targeted disruption of exons 4 and 5 of the sam68 gene, which encode the functional region of the KH-type RNA binding domain (Figure 2A).
T2528 7167-7169 TO denotes To
T2529 7170-7176 VB denotes define
T2531 7177-7180 DT denotes the
T2533 7181-7192 JJ denotes physiologic
T2532 7193-7197 NN denotes role
T2534 7198-7200 IN denotes of
T2535 7201-7206 NN denotes Sam68
T2536 7206-7208 , denotes ,
T2537 7208-7210 PRP denotes we
T2530 7211-7220 VBD denotes generated
T2538 7221-7226 NN denotes Sam68
T2540 7226-7227 HYPH denotes -
T2539 7227-7236 JJ denotes deficient
T2541 7237-7241 NNS denotes mice
T2542 7242-7244 IN denotes by
T2543 7245-7253 VBN denotes targeted
T2544 7254-7264 NN denotes disruption
T2545 7265-7267 IN denotes of
T2546 7268-7273 NNS denotes exons
T2547 7274-7275 CD denotes 4
T2548 7276-7279 CC denotes and
T2549 7280-7281 CD denotes 5
T2550 7282-7284 IN denotes of
T2551 7285-7288 DT denotes the
T2553 7289-7294 NN denotes sam68
T2552 7295-7299 NN denotes gene
T2554 7299-7301 , denotes ,
T2555 7301-7306 WDT denotes which
T2556 7307-7313 VBP denotes encode
T2557 7314-7317 DT denotes the
T2559 7318-7328 JJ denotes functional
T2558 7329-7335 NN denotes region
T2560 7336-7338 IN denotes of
T2561 7339-7342 DT denotes the
T2563 7343-7345 NN denotes KH
T2565 7345-7346 HYPH denotes -
T2564 7346-7350 NN denotes type
T2566 7351-7354 NN denotes RNA
T2567 7355-7362 NN denotes binding
T2562 7363-7369 NN denotes domain
T2568 7370-7371 -LRB- denotes (
T2570 7371-7377 NN denotes Figure
T2569 7378-7380 NN denotes 2A
T2571 7380-7381 -RRB- denotes )
T2572 7381-7382 . denotes .
T2573 7382-7516 sentence denotes The integrity of the targeted allele was verified by Southern blot analysis (Figure 2B) and by PCR of genomic DNA (unpublished data).
T2574 7383-7386 DT denotes The
T2575 7387-7396 NN denotes integrity
T2577 7397-7399 IN denotes of
T2578 7400-7403 DT denotes the
T2580 7404-7412 VBN denotes targeted
T2579 7413-7419 NN denotes allele
T2581 7420-7423 VBD denotes was
T2576 7424-7432 VBN denotes verified
T2582 7433-7435 IN denotes by
T2583 7436-7444 NNP denotes Southern
T2584 7445-7449 NN denotes blot
T2585 7450-7458 NN denotes analysis
T2586 7459-7460 -LRB- denotes (
T2588 7460-7466 NN denotes Figure
T2587 7467-7469 NN denotes 2B
T2589 7469-7470 -RRB- denotes )
T2590 7471-7474 CC denotes and
T2591 7475-7477 IN denotes by
T2592 7478-7481 NN denotes PCR
T2593 7482-7484 IN denotes of
T2594 7485-7492 JJ denotes genomic
T2595 7493-7496 NN denotes DNA
T2596 7497-7498 -LRB- denotes (
T2598 7498-7509 JJ denotes unpublished
T2597 7510-7514 NNS denotes data
T2599 7514-7515 -RRB- denotes )
T2600 7515-7516 . denotes .
T2601 7516-7816 sentence denotes Sam68 transcripts encoded by exons 1 to 5 were absent as evidenced by RT-PCR (Dataset S2), and Sam68−/− mice were devoid of Sam68 protein, as visualized by immunoblotting with anti-Sam68 AD1 and SC-333 antibodies or control normal rabbit serum or anti-Sam68 AD1 preabsorbed with peptide (Figure 2C).
T2602 7517-7522 NN denotes Sam68
T2603 7523-7534 NNS denotes transcripts
T2605 7535-7542 VBN denotes encoded
T2606 7543-7545 IN denotes by
T2607 7546-7551 NNS denotes exons
T2608 7552-7553 CD denotes 1
T2609 7554-7556 IN denotes to
T2610 7557-7558 CD denotes 5
T2604 7559-7563 VBD denotes were
T2611 7564-7570 JJ denotes absent
T2612 7571-7573 IN denotes as
T2613 7574-7583 VBN denotes evidenced
T2614 7584-7586 IN denotes by
T2615 7587-7589 NN denotes RT
T2617 7589-7590 HYPH denotes -
T2616 7590-7593 NN denotes PCR
T2618 7594-7595 -LRB- denotes (
T2620 7595-7602 NN denotes Dataset
T2619 7603-7605 NN denotes S2
T2621 7605-7606 -RRB- denotes )
T2622 7606-7608 , denotes ,
T2623 7608-7611 CC denotes and
T2624 7612-7617 NN denotes Sam68
T2626 7617-7618 SYM denotes −
T2627 7618-7619 HYPH denotes /
T2628 7619-7620 SYM denotes −
T2625 7621-7625 NNS denotes mice
T2629 7626-7630 VBD denotes were
T2630 7631-7637 JJ denotes devoid
T2631 7638-7640 IN denotes of
T2632 7641-7646 NN denotes Sam68
T2633 7647-7654 NN denotes protein
T2634 7654-7656 , denotes ,
T2635 7656-7658 IN denotes as
T2636 7659-7669 VBN denotes visualized
T2637 7670-7672 IN denotes by
T2638 7673-7687 VBG denotes immunoblotting
T2639 7688-7692 IN denotes with
T2640 7693-7703 JJ denotes anti-Sam68
T2641 7704-7707 NN denotes AD1
T2643 7708-7711 CC denotes and
T2644 7712-7714 NN denotes SC
T2645 7714-7715 HYPH denotes -
T2646 7715-7718 CD denotes 333
T2642 7719-7729 NNS denotes antibodies
T2647 7730-7732 CC denotes or
T2648 7733-7740 NN denotes control
T2650 7741-7747 JJ denotes normal
T2651 7748-7754 NN denotes rabbit
T2649 7755-7760 NN denotes serum
T2652 7761-7763 CC denotes or
T2653 7764-7774 JJ denotes anti-Sam68
T2654 7775-7778 NN denotes AD1
T2655 7779-7790 VBN denotes preabsorbed
T2656 7791-7795 IN denotes with
T2657 7796-7803 NN denotes peptide
T2658 7804-7805 -LRB- denotes (
T2660 7805-7811 NN denotes Figure
T2659 7812-7814 NN denotes 2C
T2661 7814-7815 -RRB- denotes )
T2662 7815-7816 . denotes .
T2663 7816-7924 sentence denotes SC-333 is a rabbit anti-Sam68 antibody that was raised against the C-terminal 20 amino acids of Sam68 [47].
T2664 7817-7819 NN denotes SC
T2666 7819-7820 HYPH denotes -
T2667 7820-7823 CD denotes 333
T2665 7824-7826 VBZ denotes is
T2668 7827-7828 DT denotes a
T2670 7829-7835 NN denotes rabbit
T2671 7836-7846 JJ denotes anti-Sam68
T2669 7847-7855 NN denotes antibody
T2672 7856-7860 WDT denotes that
T2674 7861-7864 VBD denotes was
T2673 7865-7871 VBN denotes raised
T2675 7872-7879 IN denotes against
T2676 7880-7883 DT denotes the
T2678 7884-7885 NN denotes C
T2680 7885-7886 HYPH denotes -
T2679 7886-7894 JJ denotes terminal
T2681 7895-7897 CD denotes 20
T2682 7898-7903 NN denotes amino
T2677 7904-7909 NNS denotes acids
T2683 7910-7912 IN denotes of
T2684 7913-7918 NN denotes Sam68
T2685 7919-7920 -LRB- denotes [
T2686 7920-7922 CD denotes 47
T2687 7922-7923 -RRB- denotes ]
T2688 7923-7924 . denotes .
T2689 7924-7991 sentence denotes These data confirmed the generation of a mouse deficient in Sam68.
T2690 7925-7930 DT denotes These
T2691 7931-7935 NNS denotes data
T2692 7936-7945 VBD denotes confirmed
T2693 7946-7949 DT denotes the
T2694 7950-7960 NN denotes generation
T2695 7961-7963 IN denotes of
T2696 7964-7965 DT denotes a
T2697 7966-7971 NN denotes mouse
T2698 7972-7981 JJ denotes deficient
T2699 7982-7984 IN denotes in
T2700 7985-7990 NN denotes Sam68
T2701 7990-7991 . denotes .
T2702 7991-8103 sentence denotes The genotypes of offspring from heterozygote intercrosses exhibited a Mendelian segregation at E18.5 (Table 1).
T2703 7992-7995 DT denotes The
T2704 7996-8005 NNS denotes genotypes
T2706 8006-8008 IN denotes of
T2707 8009-8018 NN denotes offspring
T2708 8019-8023 IN denotes from
T2709 8024-8036 NN denotes heterozygote
T2710 8037-8049 NNS denotes intercrosses
T2705 8050-8059 VBD denotes exhibited
T2711 8060-8061 DT denotes a
T2713 8062-8071 JJ denotes Mendelian
T2712 8072-8083 NN denotes segregation
T2714 8084-8086 IN denotes at
T2715 8087-8092 NN denotes E18.5
T2716 8093-8094 -LRB- denotes (
T2717 8094-8099 NN denotes Table
T2718 8100-8101 CD denotes 1
T2719 8101-8102 -RRB- denotes )
T2720 8102-8103 . denotes .
T2721 8103-8201 sentence denotes Despite the lack of visible deformity, many of the Sam68−/− pups died at birth of unknown causes.
T2722 8104-8111 IN denotes Despite
T2724 8112-8115 DT denotes the
T2725 8116-8120 NN denotes lack
T2726 8121-8123 IN denotes of
T2727 8124-8131 JJ denotes visible
T2728 8132-8141 NN denotes deformity
T2729 8141-8143 , denotes ,
T2730 8143-8147 JJ denotes many
T2731 8148-8150 IN denotes of
T2732 8151-8154 DT denotes the
T2734 8155-8160 NN denotes Sam68
T2735 8160-8161 SYM denotes −
T2736 8161-8162 HYPH denotes /
T2737 8162-8163 SYM denotes −
T2733 8164-8168 NNS denotes pups
T2723 8169-8173 VBD denotes died
T2738 8174-8176 IN denotes at
T2739 8177-8182 NN denotes birth
T2740 8183-8185 IN denotes of
T2741 8186-8193 JJ denotes unknown
T2742 8194-8200 NNS denotes causes
T2743 8200-8201 . denotes .
T2744 8201-8324 sentence denotes Sam68+/- mice were phenotypically normal and Sam68−/− pups that survived the perinatal period invariably lived to old age.
T2745 8202-8207 NN denotes Sam68
T2747 8207-8208 SYM denotes +
T2748 8208-8209 HYPH denotes /
T2749 8209-8210 SYM denotes -
T2746 8211-8215 NNS denotes mice
T2750 8216-8220 VBD denotes were
T2751 8221-8235 RB denotes phenotypically
T2752 8236-8242 JJ denotes normal
T2753 8243-8246 CC denotes and
T2754 8247-8252 NN denotes Sam68
T2756 8252-8253 SYM denotes −
T2757 8253-8254 HYPH denotes /
T2758 8254-8255 SYM denotes −
T2755 8256-8260 NNS denotes pups
T2760 8261-8265 WDT denotes that
T2761 8266-8274 VBD denotes survived
T2762 8275-8278 DT denotes the
T2764 8279-8288 JJ denotes perinatal
T2763 8289-8295 NN denotes period
T2765 8296-8306 RB denotes invariably
T2759 8307-8312 VBD denotes lived
T2766 8313-8315 IN denotes to
T2767 8316-8319 JJ denotes old
T2768 8320-8323 NN denotes age
T2769 8323-8324 . denotes .
T2770 8324-8509 sentence denotes Despite evidence that Sam68 mRNA is widely expressed and phosphorylated in mitosis [41,42], the Sam68−/− mice did not develop tumors and showed no immunologic or other major illnesses.
T2771 8325-8332 IN denotes Despite
T2773 8333-8341 NN denotes evidence
T2774 8342-8346 IN denotes that
T2776 8347-8352 NN denotes Sam68
T2777 8353-8357 NN denotes mRNA
T2778 8358-8360 VBZ denotes is
T2779 8361-8367 RB denotes widely
T2775 8368-8377 VBN denotes expressed
T2780 8378-8381 CC denotes and
T2781 8382-8396 VBN denotes phosphorylated
T2782 8397-8399 IN denotes in
T2783 8400-8407 NN denotes mitosis
T2784 8408-8409 -LRB- denotes [
T2786 8409-8411 CD denotes 41
T2787 8411-8412 , denotes ,
T2785 8412-8414 CD denotes 42
T2788 8414-8415 -RRB- denotes ]
T2789 8415-8417 , denotes ,
T2790 8417-8420 DT denotes the
T2792 8421-8426 NN denotes Sam68
T2793 8426-8427 SYM denotes −
T2794 8427-8428 HYPH denotes /
T2795 8428-8429 SYM denotes −
T2791 8430-8434 NNS denotes mice
T2796 8435-8438 VBD denotes did
T2797 8439-8442 RB denotes not
T2772 8443-8450 VB denotes develop
T2798 8451-8457 NNS denotes tumors
T2799 8458-8461 CC denotes and
T2800 8462-8468 VBD denotes showed
T2801 8469-8471 DT denotes no
T2803 8472-8483 JJ denotes immunologic
T2804 8484-8486 CC denotes or
T2805 8487-8492 JJ denotes other
T2806 8493-8498 JJ denotes major
T2802 8499-8508 NNS denotes illnesses
T2807 8508-8509 . denotes .
T2808 8509-8648 sentence denotes Sam68−/− mice did, however, have difficulty breeding due to male infertility and the females rarely provided adequate care to their young.
T2809 8510-8515 NN denotes Sam68
T2811 8515-8516 SYM denotes −
T2812 8516-8517 HYPH denotes /
T2813 8517-8518 SYM denotes −
T2810 8519-8523 NNS denotes mice
T2815 8524-8527 VBD denotes did
T2816 8527-8529 , denotes ,
T2817 8529-8536 RB denotes however
T2818 8536-8538 , denotes ,
T2814 8538-8542 VB denotes have
T2819 8543-8553 NN denotes difficulty
T2820 8554-8562 VBG denotes breeding
T2821 8563-8566 IN denotes due
T2822 8567-8569 IN denotes to
T2823 8570-8574 JJ denotes male
T2824 8575-8586 NN denotes infertility
T2825 8587-8590 CC denotes and
T2826 8591-8594 DT denotes the
T2827 8595-8602 NNS denotes females
T2829 8603-8609 RB denotes rarely
T2828 8610-8618 VBD denotes provided
T2830 8619-8627 JJ denotes adequate
T2831 8628-8632 NN denotes care
T2832 8633-8635 IN denotes to
T2833 8636-8641 PRP$ denotes their
T2834 8642-8647 JJ denotes young
T2835 8647-8648 . denotes .
T3035 9877-9882 NN denotes Sam68
T3036 9883-9893 NN denotes Deficiency
T3037 9894-9902 VBZ denotes Protects
T3038 9903-9907 NNS denotes Mice
T3039 9908-9912 IN denotes from
T3040 9913-9916 NN denotes Age
T3042 9916-9917 HYPH denotes -
T3041 9917-9924 VBN denotes Related
T3044 9925-9929 NN denotes Bone
T3043 9930-9934 NN denotes Loss
T3045 9934-10103 sentence denotes Src null animals are known to have bone metabolism defects [48], and since Sam68 is a substrate of Src, we decided to analyze the Sam68 mice for skeletal abnormalities.
T3046 9935-9938 NN denotes Src
T3048 9939-9943 JJ denotes null
T3047 9944-9951 NNS denotes animals
T3050 9952-9955 VBP denotes are
T3049 9956-9961 VBN denotes known
T3051 9962-9964 TO denotes to
T3052 9965-9969 VB denotes have
T3053 9970-9974 NN denotes bone
T3054 9975-9985 NN denotes metabolism
T3055 9986-9993 NNS denotes defects
T3056 9994-9995 -LRB- denotes [
T3057 9995-9997 CD denotes 48
T3058 9997-9998 -RRB- denotes ]
T3059 9998-10000 , denotes ,
T3060 10000-10003 CC denotes and
T3061 10004-10009 IN denotes since
T3063 10010-10015 NN denotes Sam68
T3062 10016-10018 VBZ denotes is
T3065 10019-10020 DT denotes a
T3066 10021-10030 NN denotes substrate
T3067 10031-10033 IN denotes of
T3068 10034-10037 NN denotes Src
T3069 10037-10039 , denotes ,
T3070 10039-10041 PRP denotes we
T3064 10042-10049 VBD denotes decided
T3071 10050-10052 TO denotes to
T3072 10053-10060 VB denotes analyze
T3073 10061-10064 DT denotes the
T3075 10065-10070 NN denotes Sam68
T3074 10071-10075 NNS denotes mice
T3076 10076-10079 IN denotes for
T3077 10080-10088 JJ denotes skeletal
T3078 10089-10102 NNS denotes abnormalities
T3079 10102-10103 . denotes .
T3080 10103-10209 sentence denotes Cohorts of Sam68+/+ and Sam68−/− mice were euthanized at 4 and 12 months of age for skeletal phenotyping.
T3081 10104-10111 NNS denotes Cohorts
T3083 10112-10114 IN denotes of
T3084 10115-10120 NN denotes Sam68
T3086 10120-10121 SYM denotes +
T3087 10121-10122 HYPH denotes /
T3088 10122-10123 SYM denotes +
T3089 10124-10127 CC denotes and
T3090 10128-10133 NN denotes Sam68
T3091 10133-10134 SYM denotes −
T3092 10134-10135 HYPH denotes /
T3093 10135-10136 SYM denotes −
T3085 10137-10141 NNS denotes mice
T3094 10142-10146 VBD denotes were
T3082 10147-10157 VBN denotes euthanized
T3095 10158-10160 IN denotes at
T3096 10161-10162 CD denotes 4
T3098 10163-10166 CC denotes and
T3099 10167-10169 CD denotes 12
T3097 10170-10176 NNS denotes months
T3100 10177-10179 IN denotes of
T3101 10180-10183 NN denotes age
T3102 10184-10187 IN denotes for
T3103 10188-10196 JJ denotes skeletal
T3104 10197-10208 NN denotes phenotyping
T3105 10208-10209 . denotes .
T3106 10209-10371 sentence denotes To minimize differences in the bone phenotype that might arise secondary to gender or weight differences, we selected age-matched female mice for these analyses.
T3107 10210-10212 TO denotes To
T3108 10213-10221 VB denotes minimize
T3110 10222-10233 NNS denotes differences
T3111 10234-10236 IN denotes in
T3112 10237-10240 DT denotes the
T3114 10241-10245 NN denotes bone
T3113 10246-10255 NN denotes phenotype
T3115 10256-10260 WDT denotes that
T3117 10261-10266 MD denotes might
T3116 10267-10272 VB denotes arise
T3118 10273-10282 JJ denotes secondary
T3119 10283-10285 IN denotes to
T3120 10286-10292 NN denotes gender
T3122 10293-10295 CC denotes or
T3123 10296-10302 NN denotes weight
T3121 10303-10314 NNS denotes differences
T3124 10314-10316 , denotes ,
T3125 10316-10318 PRP denotes we
T3109 10319-10327 VBD denotes selected
T3126 10328-10331 NN denotes age
T3128 10331-10332 HYPH denotes -
T3127 10332-10339 VBN denotes matched
T3130 10340-10346 JJ denotes female
T3129 10347-10351 NNS denotes mice
T3131 10352-10355 IN denotes for
T3132 10356-10361 DT denotes these
T3133 10362-10370 NNS denotes analyses
T3134 10370-10371 . denotes .
T3135 10371-10619 sentence denotes The female mice demonstrated similar increases in body weight, although 4-month-old Sam68−/− mice weighed less than Sam68+/+ mice, and similar changes in bone lengths in the axial and appendicular skeleton between 4 and 12 months of age (Table 2).
T3136 10372-10375 DT denotes The
T3138 10376-10382 JJ denotes female
T3137 10383-10387 NNS denotes mice
T3139 10388-10400 VBD denotes demonstrated
T3140 10401-10408 JJ denotes similar
T3141 10409-10418 NNS denotes increases
T3142 10419-10421 IN denotes in
T3143 10422-10426 NN denotes body
T3144 10427-10433 NN denotes weight
T3145 10433-10435 , denotes ,
T3147 10435-10443 IN denotes although
T3148 10444-10445 CD denotes 4
T3150 10445-10446 HYPH denotes -
T3149 10446-10451 NN denotes month
T3152 10451-10452 HYPH denotes -
T3151 10452-10455 JJ denotes old
T3154 10456-10461 NN denotes Sam68
T3155 10461-10462 SYM denotes −
T3156 10462-10463 HYPH denotes /
T3157 10463-10464 SYM denotes −
T3153 10465-10469 NNS denotes mice
T3146 10470-10477 VBD denotes weighed
T3158 10478-10482 JJR denotes less
T3159 10483-10487 IN denotes than
T3160 10488-10493 NN denotes Sam68
T3162 10493-10494 SYM denotes +
T3163 10494-10495 HYPH denotes /
T3164 10495-10496 SYM denotes +
T3161 10497-10501 NNS denotes mice
T3165 10501-10503 , denotes ,
T3166 10503-10506 CC denotes and
T3167 10507-10514 JJ denotes similar
T3168 10515-10522 NNS denotes changes
T3169 10523-10525 IN denotes in
T3170 10526-10530 NN denotes bone
T3171 10531-10538 NNS denotes lengths
T3172 10539-10541 IN denotes in
T3173 10542-10545 DT denotes the
T3175 10546-10551 JJ denotes axial
T3176 10552-10555 CC denotes and
T3177 10556-10568 JJ denotes appendicular
T3174 10569-10577 NN denotes skeleton
T3178 10578-10585 IN denotes between
T3179 10586-10587 CD denotes 4
T3181 10588-10591 CC denotes and
T3182 10592-10594 CD denotes 12
T3180 10595-10601 NNS denotes months
T3183 10602-10604 IN denotes of
T3184 10605-10608 NN denotes age
T3185 10609-10610 -LRB- denotes (
T3186 10610-10615 NN denotes Table
T3187 10616-10617 CD denotes 2
T3188 10617-10618 -RRB- denotes )
T3189 10618-10619 . denotes .
T3190 10619-10990 sentence denotes Faxitron radiography (Faxitron X-ray Corporation, Wheeling, Illinois, United States) (Figure 3A) and micro–computed tomography (CT) (Figure 3B) revealed significant cortical thinning (arrow) and a reduction in metaphyseal bone (asterisk) in the distal femora of 12-month-old Sam68+/+ mice compared with 4-month-old mice of either genotype and 12-month-old Sam68−/− mice.
T3191 10620-10628 NNP denotes Faxitron
T3192 10629-10640 NN denotes radiography
T3194 10641-10642 -LRB- denotes (
T3196 10642-10650 NNP denotes Faxitron
T3197 10651-10652 NN denotes X
T3199 10652-10653 HYPH denotes -
T3198 10653-10656 NN denotes ray
T3195 10657-10668 NNP denotes Corporation
T3200 10668-10670 , denotes ,
T3201 10670-10678 NNP denotes Wheeling
T3202 10678-10680 , denotes ,
T3203 10680-10688 NNP denotes Illinois
T3204 10688-10690 , denotes ,
T3205 10690-10696 NNP denotes United
T3206 10697-10703 NNP denotes States
T3207 10703-10704 -RRB- denotes )
T3208 10705-10706 -LRB- denotes (
T3210 10706-10712 NN denotes Figure
T3209 10713-10715 NN denotes 3A
T3211 10715-10716 -RRB- denotes )
T3212 10717-10720 CC denotes and
T3213 10721-10735 VBN denotes micro–computed
T3214 10736-10746 NN denotes tomography
T3215 10747-10748 -LRB- denotes (
T3216 10748-10750 NN denotes CT
T3217 10750-10751 -RRB- denotes )
T3218 10752-10753 -LRB- denotes (
T3220 10753-10759 NN denotes Figure
T3219 10760-10762 NN denotes 3B
T3221 10762-10763 -RRB- denotes )
T3193 10764-10772 VBD denotes revealed
T3222 10773-10784 JJ denotes significant
T3224 10785-10793 JJ denotes cortical
T3223 10794-10802 NN denotes thinning
T3225 10803-10804 -LRB- denotes (
T3226 10804-10809 NN denotes arrow
T3227 10809-10810 -RRB- denotes )
T3228 10811-10814 CC denotes and
T3229 10815-10816 DT denotes a
T3230 10817-10826 NN denotes reduction
T3231 10827-10829 IN denotes in
T3232 10830-10841 JJ denotes metaphyseal
T3233 10842-10846 NN denotes bone
T3234 10847-10848 -LRB- denotes (
T3235 10848-10856 NN denotes asterisk
T3236 10856-10857 -RRB- denotes )
T3237 10858-10860 IN denotes in
T3238 10861-10864 DT denotes the
T3240 10865-10871 JJ denotes distal
T3239 10872-10878 NNS denotes femora
T3241 10879-10881 IN denotes of
T3242 10882-10884 CD denotes 12
T3244 10884-10885 HYPH denotes -
T3243 10885-10890 NN denotes month
T3246 10890-10891 HYPH denotes -
T3245 10891-10894 JJ denotes old
T3248 10895-10900 NN denotes Sam68
T3249 10900-10901 SYM denotes +
T3250 10901-10902 HYPH denotes /
T3251 10902-10903 SYM denotes +
T3247 10904-10908 NNS denotes mice
T3252 10909-10917 VBN denotes compared
T3253 10918-10922 IN denotes with
T3254 10923-10924 CD denotes 4
T3256 10924-10925 HYPH denotes -
T3255 10925-10930 NN denotes month
T3258 10930-10931 HYPH denotes -
T3257 10931-10934 JJ denotes old
T3259 10935-10939 NNS denotes mice
T3260 10940-10942 IN denotes of
T3261 10943-10949 CC denotes either
T3262 10950-10958 NN denotes genotype
T3263 10959-10962 CC denotes and
T3264 10963-10965 CD denotes 12
T3266 10965-10966 HYPH denotes -
T3265 10966-10971 NN denotes month
T3268 10971-10972 HYPH denotes -
T3267 10972-10975 JJ denotes old
T3270 10976-10981 NN denotes Sam68
T3271 10981-10982 SYM denotes −
T3272 10982-10983 HYPH denotes /
T3273 10983-10984 SYM denotes −
T3269 10985-10989 NNS denotes mice
T3274 10989-10990 . denotes .
T3275 10990-11177 sentence denotes Similar reductions in trabecular bone were shown by Faxitron and micro-CT in the fifth lumbar vertebrae of the 12-month-old Sam68+/+ mice but not in the Sam68−/− mice (unpublished data).
T3276 10991-10998 JJ denotes Similar
T3277 10999-11009 NNS denotes reductions
T3279 11010-11012 IN denotes in
T3280 11013-11023 JJ denotes trabecular
T3281 11024-11028 NN denotes bone
T3282 11029-11033 VBD denotes were
T3278 11034-11039 VBN denotes shown
T3283 11040-11042 IN denotes by
T3284 11043-11051 NNP denotes Faxitron
T3285 11052-11055 CC denotes and
T3286 11056-11064 NN denotes micro-CT
T3287 11065-11067 IN denotes in
T3288 11068-11071 DT denotes the
T3290 11072-11077 JJ denotes fifth
T3291 11078-11084 NN denotes lumbar
T3289 11085-11094 NNS denotes vertebrae
T3292 11095-11097 IN denotes of
T3293 11098-11101 DT denotes the
T3295 11102-11104 CD denotes 12
T3297 11104-11105 HYPH denotes -
T3296 11105-11110 NN denotes month
T3299 11110-11111 HYPH denotes -
T3298 11111-11114 JJ denotes old
T3300 11115-11120 NN denotes Sam68
T3301 11120-11121 SYM denotes +
T3302 11121-11122 HYPH denotes /
T3303 11122-11123 SYM denotes +
T3294 11124-11128 NNS denotes mice
T3304 11129-11132 CC denotes but
T3305 11133-11136 RB denotes not
T3306 11137-11139 IN denotes in
T3307 11140-11143 DT denotes the
T3309 11144-11149 NN denotes Sam68
T3310 11149-11150 SYM denotes −
T3311 11150-11151 HYPH denotes /
T3312 11151-11152 SYM denotes −
T3308 11153-11157 NNS denotes mice
T3313 11158-11159 -LRB- denotes (
T3315 11159-11170 JJ denotes unpublished
T3314 11171-11175 NNS denotes data
T3316 11175-11176 -RRB- denotes )
T3317 11176-11177 . denotes .
T3318 11177-11460 sentence denotes Total body bone mineral content (BMC), quantified with a Lunar PIXImus mouse densitometer (GE-Lunar, Madison, Wisconsin, United States), increased in both Sam68+/+ and Sam68−/− mice between 4 months and 12 months of age, but only reached significance in the Sam68−/− mice (Table 2).
T3319 11178-11183 JJ denotes Total
T3321 11184-11188 NN denotes body
T3322 11189-11193 NN denotes bone
T3323 11194-11201 NN denotes mineral
T3320 11202-11209 NN denotes content
T3325 11210-11211 -LRB- denotes (
T3326 11211-11214 NN denotes BMC
T3327 11214-11215 -RRB- denotes )
T3328 11215-11217 , denotes ,
T3329 11217-11227 VBN denotes quantified
T3330 11228-11232 IN denotes with
T3331 11233-11234 DT denotes a
T3333 11235-11240 JJ denotes Lunar
T3334 11241-11248 NNP denotes PIXImus
T3335 11249-11254 NN denotes mouse
T3332 11255-11267 NN denotes densitometer
T3336 11268-11269 -LRB- denotes (
T3338 11269-11271 NNP denotes GE
T3339 11271-11272 HYPH denotes -
T3337 11272-11277 NNP denotes Lunar
T3340 11277-11279 , denotes ,
T3341 11279-11286 NNP denotes Madison
T3342 11286-11288 , denotes ,
T3343 11288-11297 NNP denotes Wisconsin
T3344 11297-11299 , denotes ,
T3345 11299-11305 NNP denotes United
T3346 11306-11312 NNP denotes States
T3347 11312-11313 -RRB- denotes )
T3348 11313-11315 , denotes ,
T3324 11315-11324 VBD denotes increased
T3349 11325-11327 IN denotes in
T3350 11328-11332 CC denotes both
T3351 11333-11338 NN denotes Sam68
T3353 11338-11339 SYM denotes +
T3354 11339-11340 HYPH denotes /
T3355 11340-11341 SYM denotes +
T3356 11342-11345 CC denotes and
T3357 11346-11351 NN denotes Sam68
T3358 11351-11352 SYM denotes −
T3359 11352-11353 HYPH denotes /
T3360 11353-11354 SYM denotes −
T3352 11355-11359 NNS denotes mice
T3361 11360-11367 IN denotes between
T3362 11368-11369 CD denotes 4
T3363 11370-11376 NNS denotes months
T3364 11377-11380 CC denotes and
T3365 11381-11383 CD denotes 12
T3366 11384-11390 NNS denotes months
T3367 11391-11393 IN denotes of
T3368 11394-11397 NN denotes age
T3369 11397-11399 , denotes ,
T3370 11399-11402 CC denotes but
T3371 11403-11407 RB denotes only
T3372 11408-11415 VBD denotes reached
T3373 11416-11428 NN denotes significance
T3374 11429-11431 IN denotes in
T3375 11432-11435 DT denotes the
T3377 11436-11441 NN denotes Sam68
T3378 11441-11442 SYM denotes −
T3379 11442-11443 HYPH denotes /
T3380 11443-11444 SYM denotes −
T3376 11445-11449 NNS denotes mice
T3381 11450-11451 -LRB- denotes (
T3382 11451-11456 NN denotes Table
T3383 11457-11458 CD denotes 2
T3384 11458-11459 -RRB- denotes )
T3385 11459-11460 . denotes .
T3386 11460-11554 sentence denotes Similarly, a greater increase in BMC was seen in the femur and vertebra in the Sam68−/− mice.
T3387 11461-11470 RB denotes Similarly
T3389 11470-11472 , denotes ,
T3390 11472-11473 DT denotes a
T3392 11474-11481 JJR denotes greater
T3391 11482-11490 NN denotes increase
T3393 11491-11493 IN denotes in
T3394 11494-11497 NN denotes BMC
T3395 11498-11501 VBD denotes was
T3388 11502-11506 VBN denotes seen
T3396 11507-11509 IN denotes in
T3397 11510-11513 DT denotes the
T3398 11514-11519 NN denotes femur
T3399 11520-11523 CC denotes and
T3400 11524-11532 NN denotes vertebra
T3401 11533-11535 IN denotes in
T3402 11536-11539 DT denotes the
T3404 11540-11545 NN denotes Sam68
T3405 11545-11546 SYM denotes −
T3406 11546-11547 HYPH denotes /
T3407 11547-11548 SYM denotes −
T3403 11549-11553 NNS denotes mice
T3408 11553-11554 . denotes .
T3409 11554-11760 sentence denotes Bone mineral density (BMD) remained constant or decreased in the wild type mice but increased significantly in the total body and in the femoral and vertebral regions of interest in the Sam68−/− (Table 2).
T3410 11555-11559 NN denotes Bone
T3411 11560-11567 NN denotes mineral
T3412 11568-11575 NN denotes density
T3414 11576-11577 -LRB- denotes (
T3415 11577-11580 NN denotes BMD
T3416 11580-11581 -RRB- denotes )
T3413 11582-11590 VBD denotes remained
T3417 11591-11599 JJ denotes constant
T3418 11600-11602 CC denotes or
T3419 11603-11612 VBN denotes decreased
T3420 11613-11615 IN denotes in
T3421 11616-11619 DT denotes the
T3423 11620-11624 JJ denotes wild
T3424 11625-11629 NN denotes type
T3422 11630-11634 NNS denotes mice
T3425 11635-11638 CC denotes but
T3426 11639-11648 VBD denotes increased
T3427 11649-11662 RB denotes significantly
T3428 11663-11665 IN denotes in
T3429 11666-11669 DT denotes the
T3431 11670-11675 JJ denotes total
T3430 11676-11680 NN denotes body
T3432 11681-11684 CC denotes and
T3433 11685-11687 IN denotes in
T3434 11688-11691 DT denotes the
T3436 11692-11699 JJ denotes femoral
T3437 11700-11703 CC denotes and
T3438 11704-11713 JJ denotes vertebral
T3435 11714-11721 NNS denotes regions
T3439 11722-11724 IN denotes of
T3440 11725-11733 NN denotes interest
T3441 11734-11736 IN denotes in
T3442 11737-11740 DT denotes the
T3443 11741-11746 NN denotes Sam68
T3444 11746-11747 SYM denotes −
T3445 11747-11748 HYPH denotes /
T3446 11748-11749 SYM denotes −
T3447 11750-11751 -LRB- denotes (
T3448 11751-11756 NN denotes Table
T3449 11757-11758 CD denotes 2
T3450 11758-11759 -RRB- denotes )
T3451 11759-11760 . denotes .
T3452 11760-11899 sentence denotes These data showed that Sam68−/− mice continued to thrive and accrue bone in the axial and appendicular skeleton for longer than 12 months.
T3453 11761-11766 DT denotes These
T3454 11767-11771 NNS denotes data
T3455 11772-11778 VBD denotes showed
T3456 11779-11783 IN denotes that
T3458 11784-11789 NN denotes Sam68
T3460 11789-11790 SYM denotes −
T3461 11790-11791 HYPH denotes /
T3462 11791-11792 SYM denotes −
T3459 11793-11797 NNS denotes mice
T3457 11798-11807 VBD denotes continued
T3463 11808-11810 TO denotes to
T3464 11811-11817 VB denotes thrive
T3465 11818-11821 CC denotes and
T3466 11822-11828 VB denotes accrue
T3467 11829-11833 NN denotes bone
T3468 11834-11836 IN denotes in
T3469 11837-11840 DT denotes the
T3471 11841-11846 JJ denotes axial
T3472 11847-11850 CC denotes and
T3473 11851-11863 JJ denotes appendicular
T3470 11864-11872 NN denotes skeleton
T3474 11873-11876 IN denotes for
T3475 11877-11883 JJR denotes longer
T3477 11884-11888 IN denotes than
T3476 11889-11891 CD denotes 12
T3478 11892-11898 NNS denotes months
T3479 11898-11899 . denotes .
T3480 11899-12045 sentence denotes This was in contrast to the situation in age-matched, littermate controls in which a significant amount of bone was lost over the same timeframe.
T3481 11900-11904 DT denotes This
T3482 11905-11908 VBD denotes was
T3483 11909-11911 IN denotes in
T3484 11912-11920 NN denotes contrast
T3485 11921-11923 IN denotes to
T3486 11924-11927 DT denotes the
T3487 11928-11937 NN denotes situation
T3488 11938-11940 IN denotes in
T3489 11941-11944 NN denotes age
T3491 11944-11945 HYPH denotes -
T3490 11945-11952 VBN denotes matched
T3493 11952-11954 , denotes ,
T3494 11954-11964 NN denotes littermate
T3492 11965-11973 NNS denotes controls
T3495 11974-11976 IN denotes in
T3497 11977-11982 WDT denotes which
T3498 11983-11984 DT denotes a
T3500 11985-11996 JJ denotes significant
T3499 11997-12003 NN denotes amount
T3501 12004-12006 IN denotes of
T3502 12007-12011 NN denotes bone
T3503 12012-12015 VBD denotes was
T3496 12016-12020 VBN denotes lost
T3504 12021-12025 IN denotes over
T3505 12026-12029 DT denotes the
T3507 12030-12034 JJ denotes same
T3506 12035-12044 NN denotes timeframe
T3508 12044-12045 . denotes .
T3587 13332-13337 CD denotes Three
T3589 13337-13338 HYPH denotes -
T3588 13338-13349 JJ denotes Dimensional
T3590 13350-13362 NN denotes Architecture
T3592 13363-13365 IN denotes of
T3593 13366-13370 NN denotes Bone
T3594 13371-13373 VBZ denotes Is
T3591 13374-13383 VBN denotes Preserved
T3595 13384-13386 IN denotes in
T3596 13387-13391 VBN denotes Aged
T3598 13392-13397 NN denotes Sam68
T3599 13397-13398 SYM denotes −
T3600 13398-13399 HYPH denotes /
T3601 13399-13400 SYM denotes −
T3597 13401-13405 NNS denotes Mice
T3602 13405-13627 sentence denotes Bone loss and compromised architecture are characteristic features of the skeletons of aged C57BL/6 mice and resemble the clinical features of age-related bone loss in humans that can predispose an individual to fracture.
T3603 13406-13410 NN denotes Bone
T3604 13411-13415 NN denotes loss
T3606 13416-13419 CC denotes and
T3607 13420-13431 VBN denotes compromised
T3608 13432-13444 NN denotes architecture
T3605 13445-13448 VBP denotes are
T3609 13449-13463 JJ denotes characteristic
T3610 13464-13472 NNS denotes features
T3611 13473-13475 IN denotes of
T3612 13476-13479 DT denotes the
T3613 13480-13489 NNS denotes skeletons
T3614 13490-13492 IN denotes of
T3615 13493-13497 JJ denotes aged
T3617 13498-13503 NN denotes C57BL
T3618 13503-13504 HYPH denotes /
T3619 13504-13505 CD denotes 6
T3616 13506-13510 NNS denotes mice
T3620 13511-13514 CC denotes and
T3621 13515-13523 VBP denotes resemble
T3622 13524-13527 DT denotes the
T3624 13528-13536 JJ denotes clinical
T3623 13537-13545 NNS denotes features
T3625 13546-13548 IN denotes of
T3626 13549-13552 NN denotes age
T3628 13552-13553 HYPH denotes -
T3627 13553-13560 VBN denotes related
T3630 13561-13565 NN denotes bone
T3629 13566-13570 NN denotes loss
T3631 13571-13573 IN denotes in
T3632 13574-13580 NNS denotes humans
T3633 13581-13585 WDT denotes that
T3635 13586-13589 MD denotes can
T3634 13590-13600 VB denotes predispose
T3636 13601-13603 DT denotes an
T3637 13604-13614 NN denotes individual
T3638 13615-13617 IN denotes to
T3639 13618-13626 NN denotes fracture
T3640 13626-13627 . denotes .
T3641 13627-13933 sentence denotes Quantification of the micro-CT data shown in Figure 3B confirmed the reduction in bone volume compared with tissue volume (BV/TV; Figure 4A, left) in the 12-month-old Sam68+/+ mice (hatched bars) compared with 4-month-old mice of both genotypes (solid bars) and 12-month-old Sam68−/− mice (stippled bars).
T3642 13628-13642 NN denotes Quantification
T3644 13643-13645 IN denotes of
T3645 13646-13649 DT denotes the
T3647 13650-13658 NN denotes micro-CT
T3646 13659-13663 NNS denotes data
T3648 13664-13669 VBN denotes shown
T3649 13670-13672 IN denotes in
T3650 13673-13679 NN denotes Figure
T3651 13680-13682 NN denotes 3B
T3643 13683-13692 VBD denotes confirmed
T3652 13693-13696 DT denotes the
T3653 13697-13706 NN denotes reduction
T3654 13707-13709 IN denotes in
T3655 13710-13714 NN denotes bone
T3656 13715-13721 NN denotes volume
T3657 13722-13730 VBN denotes compared
T3658 13731-13735 IN denotes with
T3659 13736-13742 NN denotes tissue
T3660 13743-13749 NN denotes volume
T3661 13750-13751 -LRB- denotes (
T3663 13751-13753 NN denotes BV
T3665 13753-13754 HYPH denotes /
T3664 13754-13756 NN denotes TV
T3666 13756-13757 : denotes ;
T3667 13758-13764 NN denotes Figure
T3662 13765-13767 NN denotes 4A
T3668 13767-13769 , denotes ,
T3669 13769-13773 JJ denotes left
T3670 13773-13774 -RRB- denotes )
T3671 13775-13777 IN denotes in
T3672 13778-13781 DT denotes the
T3674 13782-13784 CD denotes 12
T3676 13784-13785 HYPH denotes -
T3675 13785-13790 NN denotes month
T3678 13790-13791 HYPH denotes -
T3677 13791-13794 JJ denotes old
T3679 13795-13800 NN denotes Sam68
T3680 13800-13801 SYM denotes +
T3681 13801-13802 HYPH denotes /
T3682 13802-13803 SYM denotes +
T3673 13804-13808 NNS denotes mice
T3683 13809-13810 -LRB- denotes (
T3685 13810-13817 VBN denotes hatched
T3684 13818-13822 NNS denotes bars
T3686 13822-13823 -RRB- denotes )
T3687 13824-13832 VBN denotes compared
T3688 13833-13837 IN denotes with
T3689 13838-13839 CD denotes 4
T3691 13839-13840 HYPH denotes -
T3690 13840-13845 NN denotes month
T3693 13845-13846 HYPH denotes -
T3692 13846-13849 JJ denotes old
T3694 13850-13854 NNS denotes mice
T3695 13855-13857 IN denotes of
T3696 13858-13862 DT denotes both
T3697 13863-13872 NNS denotes genotypes
T3698 13873-13874 -LRB- denotes (
T3700 13874-13879 JJ denotes solid
T3699 13880-13884 NNS denotes bars
T3701 13884-13885 -RRB- denotes )
T3702 13886-13889 CC denotes and
T3703 13890-13892 CD denotes 12
T3705 13892-13893 HYPH denotes -
T3704 13893-13898 NN denotes month
T3707 13898-13899 HYPH denotes -
T3706 13899-13902 JJ denotes old
T3709 13903-13908 NN denotes Sam68
T3710 13908-13909 SYM denotes −
T3711 13909-13910 HYPH denotes /
T3712 13910-13911 SYM denotes −
T3708 13912-13916 NNS denotes mice
T3713 13917-13918 -LRB- denotes (
T3715 13918-13926 VBN denotes stippled
T3714 13927-13931 NNS denotes bars
T3716 13931-13932 -RRB- denotes )
T3717 13932-13933 . denotes .
T3718 13933-14130 sentence denotes This was associated with a significant increase (p < 0.01; denoted by the asterisks) in the structure model index, which measures the ratio of plate-like to rod-like structures (Figure 4A, right).
T3719 13934-13938 DT denotes This
T3721 13939-13942 VBD denotes was
T3720 13943-13953 VBN denotes associated
T3722 13954-13958 IN denotes with
T3723 13959-13960 DT denotes a
T3725 13961-13972 JJ denotes significant
T3724 13973-13981 NN denotes increase
T3726 13982-13983 -LRB- denotes (
T3728 13983-13984 NN denotes p
T3730 13985-13986 SYM denotes <
T3729 13987-13991 CD denotes 0.01
T3731 13991-13992 : denotes ;
T3727 13993-14000 VBN denotes denoted
T3732 14001-14003 IN denotes by
T3733 14004-14007 DT denotes the
T3734 14008-14017 NNS denotes asterisks
T3735 14017-14018 -RRB- denotes )
T3736 14019-14021 IN denotes in
T3737 14022-14025 DT denotes the
T3739 14026-14035 NN denotes structure
T3740 14036-14041 NN denotes model
T3738 14042-14047 NN denotes index
T3741 14047-14049 , denotes ,
T3742 14049-14054 WDT denotes which
T3743 14055-14063 VBZ denotes measures
T3744 14064-14067 DT denotes the
T3745 14068-14073 NN denotes ratio
T3746 14074-14076 IN denotes of
T3747 14077-14082 NN denotes plate
T3749 14082-14083 HYPH denotes -
T3748 14083-14087 JJ denotes like
T3750 14088-14090 IN denotes to
T3751 14091-14094 NN denotes rod
T3753 14094-14095 HYPH denotes -
T3752 14095-14099 JJ denotes like
T3754 14100-14110 NNS denotes structures
T3755 14111-14112 -LRB- denotes (
T3757 14112-14118 NN denotes Figure
T3756 14119-14121 NN denotes 4A
T3758 14121-14123 , denotes ,
T3759 14123-14128 NN denotes right
T3760 14128-14129 -RRB- denotes )
T3761 14129-14130 . denotes .
T3762 14130-14365 sentence denotes A quantifiable increase in the mean trabecular separation (Tb.Sp) (unpublished data) in 12-month-old Sam68+/+ mice was due to an increase in the percentage of spaces falling in the range of 350 to 700 μm (Figure 4B, red hatched line).
T3763 14131-14132 DT denotes A
T3765 14133-14145 JJ denotes quantifiable
T3764 14146-14154 NN denotes increase
T3767 14155-14157 IN denotes in
T3768 14158-14161 DT denotes the
T3770 14162-14166 NN denotes mean
T3771 14167-14177 JJ denotes trabecular
T3769 14178-14188 NN denotes separation
T3772 14189-14190 -LRB- denotes (
T3773 14190-14195 NN denotes Tb.Sp
T3774 14195-14196 -RRB- denotes )
T3775 14197-14198 -LRB- denotes (
T3777 14198-14209 JJ denotes unpublished
T3776 14210-14214 NNS denotes data
T3778 14214-14215 -RRB- denotes )
T3779 14216-14218 IN denotes in
T3780 14219-14221 CD denotes 12
T3782 14221-14222 HYPH denotes -
T3781 14222-14227 NN denotes month
T3784 14227-14228 HYPH denotes -
T3783 14228-14231 JJ denotes old
T3786 14232-14237 NN denotes Sam68
T3787 14237-14238 SYM denotes +
T3788 14238-14239 HYPH denotes /
T3789 14239-14240 SYM denotes +
T3785 14241-14245 NNS denotes mice
T3766 14246-14249 VBD denotes was
T3790 14250-14253 IN denotes due
T3791 14254-14256 IN denotes to
T3792 14257-14259 DT denotes an
T3793 14260-14268 NN denotes increase
T3794 14269-14271 IN denotes in
T3795 14272-14275 DT denotes the
T3796 14276-14286 NN denotes percentage
T3797 14287-14289 IN denotes of
T3798 14290-14296 NNS denotes spaces
T3799 14297-14304 VBG denotes falling
T3800 14305-14307 IN denotes in
T3801 14308-14311 DT denotes the
T3802 14312-14317 NN denotes range
T3803 14318-14320 IN denotes of
T3804 14321-14324 CD denotes 350
T3806 14325-14327 IN denotes to
T3805 14328-14331 CD denotes 700
T3807 14332-14334 NN denotes μm
T3808 14335-14336 -LRB- denotes (
T3810 14336-14342 NN denotes Figure
T3809 14343-14345 NN denotes 4B
T3811 14345-14347 , denotes ,
T3812 14347-14350 JJ denotes red
T3814 14351-14358 VBN denotes hatched
T3813 14359-14363 NN denotes line
T3815 14363-14364 -RRB- denotes )
T3816 14364-14365 . denotes .
T3817 14365-14459 sentence denotes Trabecular thickness remained constant among the different groups of mice (unpublished data).
T3818 14366-14376 JJ denotes Trabecular
T3819 14377-14386 NN denotes thickness
T3820 14387-14395 VBD denotes remained
T3821 14396-14404 JJ denotes constant
T3822 14405-14410 IN denotes among
T3823 14411-14414 DT denotes the
T3825 14415-14424 JJ denotes different
T3824 14425-14431 NNS denotes groups
T3826 14432-14434 IN denotes of
T3827 14435-14439 NNS denotes mice
T3828 14440-14441 -LRB- denotes (
T3830 14441-14452 JJ denotes unpublished
T3829 14453-14457 NNS denotes data
T3831 14457-14458 -RRB- denotes )
T3832 14458-14459 . denotes .
T3833 14459-14647 sentence denotes In effect, this meant that there were fewer trabeculae, rather than equivalent numbers of thin trabeculae, in the 12-month-old Sam68+/+ mice compared with any of the other groups of mice.
T3834 14460-14462 IN denotes In
T3836 14463-14469 NN denotes effect
T3837 14469-14471 , denotes ,
T3838 14471-14475 DT denotes this
T3835 14476-14481 VBD denotes meant
T3839 14482-14486 IN denotes that
T3841 14487-14492 EX denotes there
T3840 14493-14497 VBD denotes were
T3842 14498-14503 JJR denotes fewer
T3843 14504-14514 NNS denotes trabeculae
T3844 14514-14516 , denotes ,
T3845 14516-14522 RB denotes rather
T3846 14523-14527 IN denotes than
T3847 14528-14538 JJ denotes equivalent
T3848 14539-14546 NNS denotes numbers
T3849 14547-14549 IN denotes of
T3850 14550-14554 JJ denotes thin
T3851 14555-14565 NNS denotes trabeculae
T3852 14565-14567 , denotes ,
T3853 14567-14569 IN denotes in
T3854 14570-14573 DT denotes the
T3856 14574-14576 CD denotes 12
T3858 14576-14577 HYPH denotes -
T3857 14577-14582 NN denotes month
T3860 14582-14583 HYPH denotes -
T3859 14583-14586 JJ denotes old
T3861 14587-14592 NN denotes Sam68
T3862 14592-14593 SYM denotes +
T3863 14593-14594 HYPH denotes /
T3864 14594-14595 SYM denotes +
T3855 14596-14600 NNS denotes mice
T3865 14601-14609 VBN denotes compared
T3866 14610-14614 IN denotes with
T3867 14615-14618 DT denotes any
T3868 14619-14621 IN denotes of
T3869 14622-14625 DT denotes the
T3871 14626-14631 JJ denotes other
T3870 14632-14638 NNS denotes groups
T3872 14639-14641 IN denotes of
T3873 14642-14646 NNS denotes mice
T3874 14646-14647 . denotes .
T4163 15537-15541 NN denotes Bone
T4164 15542-15552 NN denotes Remodeling
T4166 15553-15555 VBZ denotes Is
T4165 15556-15565 VBN denotes Preserved
T4167 15566-15568 IN denotes in
T4168 15569-15573 VBN denotes Aged
T4170 15574-15579 NN denotes Sam68
T4171 15579-15580 SYM denotes −
T4172 15580-15581 HYPH denotes /
T4173 15581-15582 SYM denotes −
T4169 15583-15587 NNS denotes Mice
T4174 15587-15804 sentence denotes To further define the mechanisms involved in the preservation of bone mass in aged Sam68−/− mice, we prepared sections from plastic-embedded femora and tibia to evaluate osteoblast and osteoclast activity (Figure 5).
T4175 15588-15590 TO denotes To
T4177 15591-15598 RB denotes further
T4176 15599-15605 VB denotes define
T4179 15606-15609 DT denotes the
T4180 15610-15620 NNS denotes mechanisms
T4181 15621-15629 VBN denotes involved
T4182 15630-15632 IN denotes in
T4183 15633-15636 DT denotes the
T4184 15637-15649 NN denotes preservation
T4185 15650-15652 IN denotes of
T4186 15653-15657 NN denotes bone
T4187 15658-15662 NN denotes mass
T4188 15663-15665 IN denotes in
T4189 15666-15670 JJ denotes aged
T4191 15671-15676 NN denotes Sam68
T4192 15676-15677 SYM denotes −
T4193 15677-15678 HYPH denotes /
T4194 15678-15679 SYM denotes −
T4190 15680-15684 NNS denotes mice
T4195 15684-15686 , denotes ,
T4196 15686-15688 PRP denotes we
T4178 15689-15697 VBD denotes prepared
T4197 15698-15706 NNS denotes sections
T4198 15707-15711 IN denotes from
T4199 15712-15719 NN denotes plastic
T4201 15719-15720 HYPH denotes -
T4200 15720-15728 VBN denotes embedded
T4202 15729-15735 NNS denotes femora
T4203 15736-15739 CC denotes and
T4204 15740-15745 NN denotes tibia
T4205 15746-15748 TO denotes to
T4206 15749-15757 VB denotes evaluate
T4207 15758-15768 NN denotes osteoblast
T4209 15769-15772 CC denotes and
T4210 15773-15783 NN denotes osteoclast
T4208 15784-15792 NN denotes activity
T4211 15793-15794 -LRB- denotes (
T4212 15794-15800 NN denotes Figure
T4213 15801-15802 CD denotes 5
T4214 15802-15803 -RRB- denotes )
T4215 15803-15804 . denotes .
T4216 15804-16056 sentence denotes In situ enzyme histochemical staining for alkaline phosphatase (ALP; brown stain) activity was used as a biomarker for osteoblasts and tartrate-resistant ALP (tartrate-resistant acid phosphatase [TRAP]; red stain) activity as a marker for osteoclasts.
T4217 15805-15807 FW denotes In
T4218 15808-15812 FW denotes situ
T4220 15813-15819 NN denotes enzyme
T4221 15820-15833 JJ denotes histochemical
T4219 15834-15842 NN denotes staining
T4223 15843-15846 IN denotes for
T4224 15847-15855 NN denotes alkaline
T4225 15856-15867 NN denotes phosphatase
T4227 15868-15869 -LRB- denotes (
T4228 15869-15872 NN denotes ALP
T4229 15872-15873 : denotes ;
T4231 15874-15879 JJ denotes brown
T4230 15880-15885 NN denotes stain
T4232 15885-15886 -RRB- denotes )
T4226 15887-15895 NN denotes activity
T4233 15896-15899 VBD denotes was
T4222 15900-15904 VBN denotes used
T4234 15905-15907 IN denotes as
T4235 15908-15909 DT denotes a
T4236 15910-15919 NN denotes biomarker
T4237 15920-15923 IN denotes for
T4238 15924-15935 NNS denotes osteoblasts
T4239 15936-15939 CC denotes and
T4240 15940-15948 NN denotes tartrate
T4242 15948-15949 HYPH denotes -
T4241 15949-15958 JJ denotes resistant
T4244 15959-15962 NN denotes ALP
T4245 15963-15964 -LRB- denotes (
T4247 15964-15972 NN denotes tartrate
T4249 15972-15973 HYPH denotes -
T4248 15973-15982 JJ denotes resistant
T4251 15983-15987 NN denotes acid
T4250 15988-15999 NN denotes phosphatase
T4252 16000-16001 -LRB- denotes [
T4253 16001-16005 NN denotes TRAP
T4254 16005-16006 -RRB- denotes ]
T4255 16006-16007 : denotes ;
T4256 16008-16011 JJ denotes red
T4246 16012-16017 NN denotes stain
T4257 16017-16018 -RRB- denotes )
T4243 16019-16027 NN denotes activity
T4258 16028-16030 IN denotes as
T4259 16031-16032 DT denotes a
T4260 16033-16039 NN denotes marker
T4261 16040-16043 IN denotes for
T4262 16044-16055 NNS denotes osteoclasts
T4263 16055-16056 . denotes .
T4264 16056-16176 sentence denotes Little difference was seen between Sam68+/+ (Figure 5A and 5B) and Sam68−/− (Figure 5C and 5D) mice at 4 months of age.
T4265 16057-16063 JJ denotes Little
T4266 16064-16074 NN denotes difference
T4268 16075-16078 VBD denotes was
T4267 16079-16083 VBN denotes seen
T4269 16084-16091 IN denotes between
T4270 16092-16097 NN denotes Sam68
T4272 16097-16098 SYM denotes +
T4273 16098-16099 HYPH denotes /
T4274 16099-16100 SYM denotes +
T4275 16101-16102 -LRB- denotes (
T4277 16102-16108 NN denotes Figure
T4276 16109-16111 NN denotes 5A
T4278 16112-16115 CC denotes and
T4279 16116-16118 NN denotes 5B
T4280 16118-16119 -RRB- denotes )
T4281 16120-16123 CC denotes and
T4282 16124-16129 NN denotes Sam68
T4283 16129-16130 SYM denotes −
T4284 16130-16131 HYPH denotes /
T4285 16131-16132 SYM denotes −
T4286 16133-16134 -LRB- denotes (
T4288 16134-16140 NN denotes Figure
T4287 16141-16143 NN denotes 5C
T4289 16144-16147 CC denotes and
T4290 16148-16150 NN denotes 5D
T4291 16150-16151 -RRB- denotes )
T4271 16152-16156 NNS denotes mice
T4292 16157-16159 IN denotes at
T4293 16160-16161 CD denotes 4
T4294 16162-16168 NNS denotes months
T4295 16169-16171 IN denotes of
T4296 16172-16175 NN denotes age
T4297 16175-16176 . denotes .
T4298 16176-16344 sentence denotes ALP- and TRAP-positive cells were reduced in the 12-month-old Sam68+/+ mice (Figure 5E and 5F) and remained unchanged in 12-month-old Sam68−/− mice (Figure 5G and 5H).
T4299 16177-16180 NN denotes ALP
T4301 16180-16181 HYPH denotes -
T4302 16182-16185 CC denotes and
T4303 16186-16190 NN denotes TRAP
T4304 16190-16191 HYPH denotes -
T4300 16191-16199 JJ denotes positive
T4305 16200-16205 NNS denotes cells
T4307 16206-16210 VBD denotes were
T4306 16211-16218 VBN denotes reduced
T4308 16219-16221 IN denotes in
T4309 16222-16225 DT denotes the
T4311 16226-16228 CD denotes 12
T4313 16228-16229 HYPH denotes -
T4312 16229-16234 NN denotes month
T4315 16234-16235 HYPH denotes -
T4314 16235-16238 JJ denotes old
T4316 16239-16244 NN denotes Sam68
T4317 16244-16245 SYM denotes +
T4318 16245-16246 HYPH denotes /
T4319 16246-16247 SYM denotes +
T4310 16248-16252 NNS denotes mice
T4320 16253-16254 -LRB- denotes (
T4322 16254-16260 NN denotes Figure
T4321 16261-16263 NN denotes 5E
T4323 16264-16267 CC denotes and
T4324 16268-16270 NN denotes 5F
T4325 16270-16271 -RRB- denotes )
T4326 16272-16275 CC denotes and
T4327 16276-16284 VBD denotes remained
T4328 16285-16294 JJ denotes unchanged
T4329 16295-16297 IN denotes in
T4330 16298-16300 CD denotes 12
T4332 16300-16301 HYPH denotes -
T4331 16301-16306 NN denotes month
T4334 16306-16307 HYPH denotes -
T4333 16307-16310 JJ denotes old
T4336 16311-16316 NN denotes Sam68
T4337 16316-16317 SYM denotes −
T4338 16317-16318 HYPH denotes /
T4339 16318-16319 SYM denotes −
T4335 16320-16324 NNS denotes mice
T4340 16325-16326 -LRB- denotes (
T4342 16326-16332 NN denotes Figure
T4341 16333-16335 NN denotes 5G
T4343 16336-16339 CC denotes and
T4344 16340-16342 NN denotes 5H
T4345 16342-16343 -RRB- denotes )
T4346 16343-16344 . denotes .
T4347 16344-16582 sentence denotes The reduction in both osteoblast and osteoclast activity in the 12-month-old Sam68+/+ mice argued against bone being lost primarily due to a relative increase in osteoclast over osteoblast activity, as seen in high turnover disease [49].
T4348 16345-16348 DT denotes The
T4349 16349-16358 NN denotes reduction
T4351 16359-16361 IN denotes in
T4352 16362-16366 CC denotes both
T4353 16367-16377 NN denotes osteoblast
T4355 16378-16381 CC denotes and
T4356 16382-16392 NN denotes osteoclast
T4354 16393-16401 NN denotes activity
T4357 16402-16404 IN denotes in
T4358 16405-16408 DT denotes the
T4360 16409-16411 CD denotes 12
T4362 16411-16412 HYPH denotes -
T4361 16412-16417 NN denotes month
T4364 16417-16418 HYPH denotes -
T4363 16418-16421 JJ denotes old
T4365 16422-16427 NN denotes Sam68
T4366 16427-16428 SYM denotes +
T4367 16428-16429 HYPH denotes /
T4368 16429-16430 SYM denotes +
T4359 16431-16435 NNS denotes mice
T4350 16436-16442 VBD denotes argued
T4369 16443-16450 IN denotes against
T4370 16451-16455 NN denotes bone
T4372 16456-16461 VBG denotes being
T4371 16462-16466 VBN denotes lost
T4373 16467-16476 RB denotes primarily
T4374 16477-16480 IN denotes due
T4375 16481-16483 IN denotes to
T4376 16484-16485 DT denotes a
T4378 16486-16494 JJ denotes relative
T4377 16495-16503 NN denotes increase
T4379 16504-16506 IN denotes in
T4380 16507-16517 NN denotes osteoclast
T4381 16518-16522 IN denotes over
T4382 16523-16533 NN denotes osteoblast
T4383 16534-16542 NN denotes activity
T4384 16542-16544 , denotes ,
T4385 16544-16546 IN denotes as
T4386 16547-16551 VBN denotes seen
T4387 16552-16554 IN denotes in
T4388 16555-16559 JJ denotes high
T4389 16560-16568 NN denotes turnover
T4390 16569-16576 NN denotes disease
T4391 16577-16578 -LRB- denotes [
T4392 16578-16580 CD denotes 49
T4393 16580-16581 -RRB- denotes ]
T4394 16581-16582 . denotes .
T4395 16582-17336 sentence denotes Figure 5 Histologic Analysis of Undecalcified Bone from Sam68+/+ and Sam68−/− Mice Sections of tibia fixed in 4% paraformaldehyde and embedded in plastic were stained for ALP (A–C, E–G) activity to identify osteoblasts or for TRAP (B–D, F–H) activity to identify osteoclasts. Staining patterns were similar in 4-month-old Sam68+/+ (A and B), 4-month-old Sam68−/− (C and D), and 12-month-old Sam68−/− (G and H) mice compared with 12-month-old Sam68+/+ mice (E and F). Magnification at source, left panels ×10 and right panels ×40. Micrographs are representative of those taken from five to seven sections in each group of animals. Histomorphometric analyses [50] of the long bones (Table 3) corroborated the radiologic evidence of age-related bone loss.
T4396 17214-17231 JJ denotes Histomorphometric
T4397 17232-17240 NNS denotes analyses
T4399 17241-17242 -LRB- denotes [
T4400 17242-17244 CD denotes 50
T4401 17244-17245 -RRB- denotes ]
T4402 17246-17248 IN denotes of
T4403 17249-17252 DT denotes the
T4405 17253-17257 JJ denotes long
T4404 17258-17263 NNS denotes bones
T4406 17264-17265 -LRB- denotes (
T4407 17265-17270 NN denotes Table
T4408 17271-17272 CD denotes 3
T4409 17272-17273 -RRB- denotes )
T4398 17274-17286 VBD denotes corroborated
T4410 17287-17290 DT denotes the
T4412 17291-17301 JJ denotes radiologic
T4411 17302-17310 NN denotes evidence
T4413 17311-17313 IN denotes of
T4414 17314-17317 NN denotes age
T4416 17317-17318 HYPH denotes -
T4415 17318-17325 VBN denotes related
T4418 17326-17330 NN denotes bone
T4417 17331-17335 NN denotes loss
T4419 17335-17336 . denotes .
T4420 17336-17523 sentence denotes Bone volume (BV/TV), newly formed osteoid (OV/TV), and mineral apposition rate (MAR) were all significantly reduced in 12-month-old Sam68+/+ mice compared with 4-month-old Sam68+/+ mice.
T4421 17337-17341 NN denotes Bone
T4422 17342-17348 NN denotes volume
T4424 17349-17350 -LRB- denotes (
T4425 17350-17352 NN denotes BV
T4427 17352-17353 HYPH denotes /
T4426 17353-17355 NN denotes TV
T4428 17355-17356 -RRB- denotes )
T4429 17356-17358 , denotes ,
T4430 17358-17363 RB denotes newly
T4431 17364-17370 VBN denotes formed
T4432 17371-17378 JJ denotes osteoid
T4433 17379-17380 -LRB- denotes (
T4434 17380-17382 NN denotes OV
T4436 17382-17383 HYPH denotes /
T4435 17383-17385 NN denotes TV
T4437 17385-17386 -RRB- denotes )
T4438 17386-17388 , denotes ,
T4439 17388-17391 CC denotes and
T4440 17392-17399 NN denotes mineral
T4442 17400-17410 NN denotes apposition
T4441 17411-17415 NN denotes rate
T4443 17416-17417 -LRB- denotes (
T4444 17417-17420 NN denotes MAR
T4445 17420-17421 -RRB- denotes )
T4446 17422-17426 VBD denotes were
T4447 17427-17430 DT denotes all
T4448 17431-17444 RB denotes significantly
T4423 17445-17452 VBN denotes reduced
T4449 17453-17455 IN denotes in
T4450 17456-17458 CD denotes 12
T4452 17458-17459 HYPH denotes -
T4451 17459-17464 NN denotes month
T4454 17464-17465 HYPH denotes -
T4453 17465-17468 JJ denotes old
T4456 17469-17474 NN denotes Sam68
T4457 17474-17475 SYM denotes +
T4458 17475-17476 HYPH denotes /
T4459 17476-17477 SYM denotes +
T4455 17478-17482 NNS denotes mice
T4460 17483-17491 VBN denotes compared
T4461 17492-17496 IN denotes with
T4462 17497-17498 CD denotes 4
T4464 17498-17499 HYPH denotes -
T4463 17499-17504 NN denotes month
T4466 17504-17505 HYPH denotes -
T4465 17505-17508 JJ denotes old
T4468 17509-17514 NN denotes Sam68
T4469 17514-17515 SYM denotes +
T4470 17515-17516 HYPH denotes /
T4471 17516-17517 SYM denotes +
T4467 17518-17522 NNS denotes mice
T4472 17522-17523 . denotes .
T4473 17523-17706 sentence denotes These reductions were associated with a significant increase in marrow fat (FV/TV) and decreased numbers of osteoblasts (nOB/TV) and osteoclasts (nOC/TV) per tissue volume (Table 3).
T4474 17524-17529 DT denotes These
T4475 17530-17540 NNS denotes reductions
T4477 17541-17545 VBD denotes were
T4476 17546-17556 VBN denotes associated
T4478 17557-17561 IN denotes with
T4479 17562-17563 DT denotes a
T4481 17564-17575 JJ denotes significant
T4480 17576-17584 NN denotes increase
T4482 17585-17587 IN denotes in
T4483 17588-17594 NN denotes marrow
T4484 17595-17598 NN denotes fat
T4485 17599-17600 -LRB- denotes (
T4487 17600-17602 NN denotes FV
T4488 17602-17603 HYPH denotes /
T4486 17603-17605 NN denotes TV
T4489 17605-17606 -RRB- denotes )
T4490 17607-17610 CC denotes and
T4491 17611-17620 VBN denotes decreased
T4492 17621-17628 NNS denotes numbers
T4493 17629-17631 IN denotes of
T4494 17632-17643 NNS denotes osteoblasts
T4495 17644-17645 -LRB- denotes (
T4497 17645-17648 NN denotes nOB
T4498 17648-17649 HYPH denotes /
T4496 17649-17651 NN denotes TV
T4499 17651-17652 -RRB- denotes )
T4500 17653-17656 CC denotes and
T4501 17657-17668 NNS denotes osteoclasts
T4502 17669-17670 -LRB- denotes (
T4504 17670-17673 NN denotes nOC
T4505 17673-17674 HYPH denotes /
T4503 17674-17676 NN denotes TV
T4506 17676-17677 -RRB- denotes )
T4507 17678-17681 IN denotes per
T4508 17682-17688 NN denotes tissue
T4509 17689-17695 NN denotes volume
T4510 17696-17697 -LRB- denotes (
T4511 17697-17702 NN denotes Table
T4512 17703-17704 CD denotes 3
T4513 17704-17705 -RRB- denotes )
T4514 17705-17706 . denotes .
T4515 17706-17905 sentence denotes These results were in contrast to those of the 12-month-old Sam68−/− mice in which all histomorphometric parameters, including marrow fat, resembled those of young mice of either genotype (Table 3).
T4516 17707-17712 DT denotes These
T4517 17713-17720 NNS denotes results
T4518 17721-17725 VBD denotes were
T4519 17726-17728 IN denotes in
T4520 17729-17737 NN denotes contrast
T4521 17738-17740 IN denotes to
T4522 17741-17746 DT denotes those
T4523 17747-17749 IN denotes of
T4524 17750-17753 DT denotes the
T4526 17754-17756 CD denotes 12
T4528 17756-17757 HYPH denotes -
T4527 17757-17762 NN denotes month
T4530 17762-17763 HYPH denotes -
T4529 17763-17766 JJ denotes old
T4531 17767-17772 NN denotes Sam68
T4532 17772-17773 SYM denotes −
T4533 17773-17774 HYPH denotes /
T4534 17774-17775 SYM denotes −
T4525 17776-17780 NNS denotes mice
T4535 17781-17783 IN denotes in
T4537 17784-17789 WDT denotes which
T4538 17790-17793 DT denotes all
T4540 17794-17811 JJ denotes histomorphometric
T4539 17812-17822 NNS denotes parameters
T4541 17822-17824 , denotes ,
T4542 17824-17833 VBG denotes including
T4543 17834-17840 NN denotes marrow
T4544 17841-17844 NN denotes fat
T4545 17844-17846 , denotes ,
T4536 17846-17855 VBD denotes resembled
T4546 17856-17861 DT denotes those
T4547 17862-17864 IN denotes of
T4548 17865-17870 JJ denotes young
T4549 17871-17875 NNS denotes mice
T4550 17876-17878 IN denotes of
T4551 17879-17885 CC denotes either
T4552 17886-17894 NN denotes genotype
T4553 17895-17896 -LRB- denotes (
T4554 17896-17901 NN denotes Table
T4555 17902-17903 CD denotes 3
T4556 17903-17904 -RRB- denotes )
T4557 17904-17905 . denotes .
T4558 17905-18112 sentence denotes When cells were expressed as a function of the bone perimeter (nOB/BP, nOC/BP), there were no statistical differences and the ratio of osteoblasts to osteoclasts was similar in all groups of mice (Table 3).
T4559 17906-17910 WRB denotes When
T4561 17911-17916 NNS denotes cells
T4562 17917-17921 VBD denotes were
T4560 17922-17931 VBN denotes expressed
T4564 17932-17934 IN denotes as
T4565 17935-17936 DT denotes a
T4566 17937-17945 NN denotes function
T4567 17946-17948 IN denotes of
T4568 17949-17952 DT denotes the
T4570 17953-17957 NN denotes bone
T4569 17958-17967 NN denotes perimeter
T4571 17968-17969 -LRB- denotes (
T4573 17969-17972 NN denotes nOB
T4574 17972-17973 HYPH denotes /
T4572 17973-17975 NN denotes BP
T4575 17975-17977 , denotes ,
T4576 17977-17980 NN denotes nOC
T4578 17980-17981 HYPH denotes /
T4577 17981-17983 NN denotes BP
T4579 17983-17984 -RRB- denotes )
T4580 17984-17986 , denotes ,
T4581 17986-17991 EX denotes there
T4563 17992-17996 VBD denotes were
T4582 17997-17999 DT denotes no
T4584 18000-18011 JJ denotes statistical
T4583 18012-18023 NNS denotes differences
T4585 18024-18027 CC denotes and
T4586 18028-18031 DT denotes the
T4587 18032-18037 NN denotes ratio
T4589 18038-18040 IN denotes of
T4590 18041-18052 NNS denotes osteoblasts
T4591 18053-18055 IN denotes to
T4592 18056-18067 NNS denotes osteoclasts
T4588 18068-18071 VBD denotes was
T4593 18072-18079 JJ denotes similar
T4594 18080-18082 IN denotes in
T4595 18083-18086 DT denotes all
T4596 18087-18093 NNS denotes groups
T4597 18094-18096 IN denotes of
T4598 18097-18101 NNS denotes mice
T4599 18102-18103 -LRB- denotes (
T4600 18103-18108 NN denotes Table
T4601 18109-18110 CD denotes 3
T4602 18110-18111 -RRB- denotes )
T4603 18111-18112 . denotes .
T4604 18112-18406 sentence denotes Table 3 Histomorphometric Analysis of Long Bones from 4- and 12-Month-Old Mice Circulating levels of serum C-telopeptide (sCTX; Roche Diagnostics, Mannheim, Germany) and ALP showed little difference among the groups, except for a small decrease in 12-month-old Sam68−/− mice and (Dataset S3).
T4605 18193-18204 VBG denotes Circulating
T4606 18205-18211 NNS denotes levels
T4608 18212-18214 IN denotes of
T4609 18215-18220 NN denotes serum
T4611 18221-18222 NN denotes C
T4612 18222-18223 HYPH denotes -
T4610 18223-18234 NN denotes telopeptide
T4613 18235-18236 -LRB- denotes (
T4615 18236-18240 NN denotes sCTX
T4616 18240-18241 : denotes ;
T4617 18242-18247 NNP denotes Roche
T4614 18248-18259 NNP denotes Diagnostics
T4618 18259-18261 , denotes ,
T4619 18261-18269 NNP denotes Mannheim
T4620 18269-18271 , denotes ,
T4621 18271-18278 NNP denotes Germany
T4622 18278-18279 -RRB- denotes )
T4623 18280-18283 CC denotes and
T4624 18284-18287 NN denotes ALP
T4607 18288-18294 VBD denotes showed
T4625 18295-18301 JJ denotes little
T4626 18302-18312 NN denotes difference
T4627 18313-18318 IN denotes among
T4628 18319-18322 DT denotes the
T4629 18323-18329 NNS denotes groups
T4630 18329-18331 , denotes ,
T4631 18331-18337 IN denotes except
T4632 18338-18341 IN denotes for
T4633 18342-18343 DT denotes a
T4635 18344-18349 JJ denotes small
T4634 18350-18358 NN denotes decrease
T4636 18359-18361 IN denotes in
T4637 18362-18364 CD denotes 12
T4639 18364-18365 HYPH denotes -
T4638 18365-18370 NN denotes month
T4641 18370-18371 HYPH denotes -
T4640 18371-18374 JJ denotes old
T4643 18375-18380 NN denotes Sam68
T4644 18380-18381 SYM denotes −
T4645 18381-18382 HYPH denotes /
T4646 18382-18383 SYM denotes −
T4642 18384-18388 NNS denotes mice
T4647 18389-18392 CC denotes and
T4648 18393-18394 -LRB- denotes (
T4650 18394-18401 NN denotes Dataset
T4649 18402-18404 NN denotes S3
T4651 18404-18405 -RRB- denotes )
T4652 18405-18406 . denotes .
T4653 18406-18562 sentence denotes Serum estrogen was significantly decreased in all 12-month-old mice regardless of genotype with a reciprocal increase in interleukin-6 levels (Dataset S3).
T4654 18407-18412 NN denotes Serum
T4655 18413-18421 NN denotes estrogen
T4657 18422-18425 VBD denotes was
T4658 18426-18439 RB denotes significantly
T4656 18440-18449 VBN denotes decreased
T4659 18450-18452 IN denotes in
T4660 18453-18456 DT denotes all
T4662 18457-18459 CD denotes 12
T4664 18459-18460 HYPH denotes -
T4663 18460-18465 NN denotes month
T4666 18465-18466 HYPH denotes -
T4665 18466-18469 JJ denotes old
T4661 18470-18474 NNS denotes mice
T4667 18475-18485 RB denotes regardless
T4668 18486-18488 IN denotes of
T4669 18489-18497 NN denotes genotype
T4670 18498-18502 IN denotes with
T4671 18503-18504 DT denotes a
T4673 18505-18515 JJ denotes reciprocal
T4672 18516-18524 NN denotes increase
T4674 18525-18527 IN denotes in
T4675 18528-18539 NN denotes interleukin
T4677 18539-18540 HYPH denotes -
T4678 18540-18541 CD denotes 6
T4676 18542-18548 NNS denotes levels
T4679 18549-18550 -LRB- denotes (
T4681 18550-18557 NN denotes Dataset
T4680 18558-18560 NN denotes S3
T4682 18560-18561 -RRB- denotes )
T4683 18561-18562 . denotes .
T4684 18562-18685 sentence denotes Given the involvement of leptin in regulating body and bone mass, serum leptin was measured in Sam68+/+ and Sam68−/− mice.
T4685 18563-18568 VBN denotes Given
T4687 18569-18572 DT denotes the
T4688 18573-18584 NN denotes involvement
T4689 18585-18587 IN denotes of
T4690 18588-18594 NN denotes leptin
T4691 18595-18597 IN denotes in
T4692 18598-18608 VBG denotes regulating
T4693 18609-18613 NN denotes body
T4695 18614-18617 CC denotes and
T4696 18618-18622 NN denotes bone
T4694 18623-18627 NN denotes mass
T4697 18627-18629 , denotes ,
T4698 18629-18634 NN denotes serum
T4699 18635-18641 NN denotes leptin
T4700 18642-18645 VBD denotes was
T4686 18646-18654 VBN denotes measured
T4701 18655-18657 IN denotes in
T4702 18658-18663 NN denotes Sam68
T4704 18663-18664 SYM denotes +
T4705 18664-18665 HYPH denotes /
T4706 18665-18666 SYM denotes +
T4707 18667-18670 CC denotes and
T4708 18671-18676 NN denotes Sam68
T4709 18676-18677 SYM denotes −
T4710 18677-18678 HYPH denotes /
T4711 18678-18679 SYM denotes −
T4703 18680-18684 NNS denotes mice
T4712 18684-18685 . denotes .
T4713 18685-18818 sentence denotes Twelve-month-old Sam68−/− mice had significantly lower levels than young and old Sam68+/+ mice and young Sam68−/− mice (Dataset S3).
T4714 18686-18692 CD denotes Twelve
T4716 18692-18693 HYPH denotes -
T4715 18693-18698 NN denotes month
T4718 18698-18699 HYPH denotes -
T4717 18699-18702 JJ denotes old
T4720 18703-18708 NN denotes Sam68
T4721 18708-18709 SYM denotes −
T4722 18709-18710 HYPH denotes /
T4723 18710-18711 SYM denotes −
T4719 18712-18716 NNS denotes mice
T4724 18717-18720 VBD denotes had
T4725 18721-18734 RB denotes significantly
T4726 18735-18740 JJR denotes lower
T4727 18741-18747 NNS denotes levels
T4728 18748-18752 IN denotes than
T4729 18753-18758 JJ denotes young
T4731 18759-18762 CC denotes and
T4732 18763-18766 JJ denotes old
T4733 18767-18772 NN denotes Sam68
T4734 18772-18773 SYM denotes +
T4735 18773-18774 HYPH denotes /
T4736 18774-18775 SYM denotes +
T4730 18776-18780 NNS denotes mice
T4737 18781-18784 CC denotes and
T4738 18785-18790 JJ denotes young
T4740 18791-18796 NN denotes Sam68
T4741 18796-18797 SYM denotes −
T4742 18797-18798 HYPH denotes /
T4743 18798-18799 SYM denotes −
T4739 18800-18804 NNS denotes mice
T4744 18805-18806 -LRB- denotes (
T4746 18806-18813 NN denotes Dataset
T4745 18814-18816 NN denotes S3
T4747 18816-18817 -RRB- denotes )
T4748 18817-18818 . denotes .
T4749 18818-19040 sentence denotes Taken together, these observations suggested that preservation of bone mass in the Sam68−/− mice was not due primarily to altered estrogen status but could have been influenced by differences in circulating leptin levels.
T4750 18819-18824 VBN denotes Taken
T4752 18825-18833 RB denotes together
T4753 18833-18835 , denotes ,
T4754 18835-18840 DT denotes these
T4755 18841-18853 NNS denotes observations
T4751 18854-18863 VBD denotes suggested
T4756 18864-18868 IN denotes that
T4758 18869-18881 NN denotes preservation
T4759 18882-18884 IN denotes of
T4760 18885-18889 NN denotes bone
T4761 18890-18894 NN denotes mass
T4762 18895-18897 IN denotes in
T4763 18898-18901 DT denotes the
T4765 18902-18907 NN denotes Sam68
T4766 18907-18908 SYM denotes −
T4767 18908-18909 HYPH denotes /
T4768 18909-18910 SYM denotes −
T4764 18911-18915 NNS denotes mice
T4757 18916-18919 VBD denotes was
T4769 18920-18923 RB denotes not
T4770 18924-18927 IN denotes due
T4771 18928-18937 RB denotes primarily
T4772 18938-18940 IN denotes to
T4773 18941-18948 VBN denotes altered
T4775 18949-18957 NN denotes estrogen
T4774 18958-18964 NN denotes status
T4776 18965-18968 CC denotes but
T4777 18969-18974 MD denotes could
T4779 18975-18979 VB denotes have
T4780 18980-18984 VBN denotes been
T4778 18985-18995 VBN denotes influenced
T4781 18996-18998 IN denotes by
T4782 18999-19010 NNS denotes differences
T4783 19011-19013 IN denotes in
T4784 19014-19025 VBG denotes circulating
T4786 19026-19032 NN denotes leptin
T4785 19033-19039 NNS denotes levels
T4787 19039-19040 . denotes .
T4956 19042-19052 NN denotes Osteoblast
T4958 19052-19054 , denotes ,
T4959 19054-19057 CC denotes but
T4960 19058-19061 RB denotes Not
T4961 19062-19072 NN denotes Osteoclast
T4962 19072-19074 , denotes ,
T4957 19074-19082 NN denotes Activity
T4964 19083-19085 VBZ denotes Is
T4963 19086-19093 VBN denotes Altered
T4965 19094-19096 IN denotes in
T4966 19097-19102 NN denotes Sam68
T4968 19102-19103 SYM denotes −
T4969 19103-19104 HYPH denotes /
T4970 19104-19105 SYM denotes −
T4967 19106-19110 NNS denotes Mice
T4971 19111-19113 FW denotes Ex
T4972 19114-19118 FW denotes Vivo
T4973 19118-19297 sentence denotes Maintenance of bone mass during the adult remodeling cycle is dependent on the coupling of osteoblast to osteoclast activity, such that there is no net gain or loss of bone [49].
T4974 19119-19130 NN denotes Maintenance
T4976 19131-19133 IN denotes of
T4977 19134-19138 NN denotes bone
T4978 19139-19143 NN denotes mass
T4979 19144-19150 IN denotes during
T4980 19151-19154 DT denotes the
T4982 19155-19160 JJ denotes adult
T4983 19161-19171 NN denotes remodeling
T4981 19172-19177 NN denotes cycle
T4975 19178-19180 VBZ denotes is
T4984 19181-19190 JJ denotes dependent
T4985 19191-19193 IN denotes on
T4986 19194-19197 DT denotes the
T4987 19198-19206 NN denotes coupling
T4988 19207-19209 IN denotes of
T4989 19210-19220 NN denotes osteoblast
T4990 19221-19223 IN denotes to
T4991 19224-19234 NN denotes osteoclast
T4992 19235-19243 NN denotes activity
T4993 19243-19245 , denotes ,
T4994 19245-19249 JJ denotes such
T4996 19250-19254 IN denotes that
T4997 19255-19260 EX denotes there
T4995 19261-19263 VBZ denotes is
T4998 19264-19266 DT denotes no
T5000 19267-19270 JJ denotes net
T4999 19271-19275 NN denotes gain
T5001 19276-19278 CC denotes or
T5002 19279-19283 NN denotes loss
T5003 19284-19286 IN denotes of
T5004 19287-19291 NN denotes bone
T5005 19292-19293 -LRB- denotes [
T5006 19293-19295 CD denotes 49
T5007 19295-19296 -RRB- denotes ]
T5008 19296-19297 . denotes .
T5009 19297-19498 sentence denotes To further explore the age-related advantage of Sam68−/− mice with respect to bone preservation, we examined the functional activity of Sam68−/− osteoblasts and osteoclasts ex vivo (Figure 6A and 6B).
T5010 19298-19300 TO denotes To
T5012 19301-19308 RB denotes further
T5011 19309-19316 VB denotes explore
T5014 19317-19320 DT denotes the
T5016 19321-19324 NN denotes age
T5018 19324-19325 HYPH denotes -
T5017 19325-19332 VBN denotes related
T5015 19333-19342 NN denotes advantage
T5019 19343-19345 IN denotes of
T5020 19346-19351 NN denotes Sam68
T5022 19351-19352 SYM denotes −
T5023 19352-19353 HYPH denotes /
T5024 19353-19354 SYM denotes −
T5021 19355-19359 NNS denotes mice
T5025 19360-19364 IN denotes with
T5026 19365-19372 NN denotes respect
T5027 19373-19375 IN denotes to
T5028 19376-19380 NN denotes bone
T5029 19381-19393 NN denotes preservation
T5030 19393-19395 , denotes ,
T5031 19395-19397 PRP denotes we
T5013 19398-19406 VBD denotes examined
T5032 19407-19410 DT denotes the
T5034 19411-19421 JJ denotes functional
T5033 19422-19430 NN denotes activity
T5035 19431-19433 IN denotes of
T5036 19434-19439 NN denotes Sam68
T5037 19439-19440 SYM denotes −
T5038 19440-19441 HYPH denotes /
T5039 19441-19442 SYM denotes −
T5040 19443-19454 NNS denotes osteoblasts
T5041 19455-19458 CC denotes and
T5042 19459-19470 NNS denotes osteoclasts
T5043 19471-19473 FW denotes ex
T5044 19474-19478 FW denotes vivo
T5045 19479-19480 -LRB- denotes (
T5047 19480-19486 NN denotes Figure
T5046 19487-19489 NN denotes 6A
T5048 19490-19493 CC denotes and
T5049 19494-19496 NN denotes 6B
T5050 19496-19497 -RRB- denotes )
T5051 19497-19498 . denotes .
T5052 19498-19921 sentence denotes Cultures of bone marrow stromal cells harvested from 4-week-old Sam68−/− mice and maintained for 18 days in osteoblast differentiation medium demonstrated more intense staining for ALP at 6 days (unpublished data) and 18 days (Figure 6A), and more mineralized nodules at 18 days, than the Sam68+/+ mice, even though similar amounts of fibroblast colony-forming units (CFU-F) were observed (Figure 6A and unpublished data).
T5053 19499-19507 NNS denotes Cultures
T5055 19508-19510 IN denotes of
T5056 19511-19515 NN denotes bone
T5057 19516-19522 NN denotes marrow
T5059 19523-19530 JJ denotes stromal
T5058 19531-19536 NNS denotes cells
T5060 19537-19546 VBN denotes harvested
T5061 19547-19551 IN denotes from
T5062 19552-19553 CD denotes 4
T5064 19553-19554 HYPH denotes -
T5063 19554-19558 NN denotes week
T5066 19558-19559 HYPH denotes -
T5065 19559-19562 JJ denotes old
T5068 19563-19568 NN denotes Sam68
T5069 19568-19569 SYM denotes −
T5070 19569-19570 HYPH denotes /
T5071 19570-19571 SYM denotes −
T5067 19572-19576 NNS denotes mice
T5072 19577-19580 CC denotes and
T5073 19581-19591 VBN denotes maintained
T5074 19592-19595 IN denotes for
T5075 19596-19598 CD denotes 18
T5076 19599-19603 NNS denotes days
T5077 19604-19606 IN denotes in
T5078 19607-19617 NN denotes osteoblast
T5079 19618-19633 NN denotes differentiation
T5080 19634-19640 NN denotes medium
T5054 19641-19653 VBD denotes demonstrated
T5081 19654-19658 RBR denotes more
T5082 19659-19666 JJ denotes intense
T5083 19667-19675 NN denotes staining
T5084 19676-19679 IN denotes for
T5085 19680-19683 NN denotes ALP
T5086 19684-19686 IN denotes at
T5087 19687-19688 CD denotes 6
T5088 19689-19693 NNS denotes days
T5089 19694-19695 -LRB- denotes (
T5091 19695-19706 JJ denotes unpublished
T5090 19707-19711 NNS denotes data
T5092 19711-19712 -RRB- denotes )
T5093 19713-19716 CC denotes and
T5094 19717-19719 CD denotes 18
T5095 19720-19724 NNS denotes days
T5096 19725-19726 -LRB- denotes (
T5098 19726-19732 NN denotes Figure
T5097 19733-19735 NN denotes 6A
T5099 19735-19736 NN denotes )
T5100 19736-19738 NN denotes ,
T5101 19738-19741 CC denotes and
T5102 19742-19746 JJR denotes more
T5104 19747-19758 VBN denotes mineralized
T5103 19759-19766 NNS denotes nodules
T5105 19767-19769 IN denotes at
T5106 19770-19772 CD denotes 18
T5107 19773-19777 NNS denotes days
T5108 19777-19779 NN denotes ,
T5109 19779-19783 IN denotes than
T5110 19784-19787 DT denotes the
T5112 19788-19793 NN denotes Sam68
T5113 19793-19794 SYM denotes +
T5114 19794-19795 HYPH denotes /
T5115 19795-19796 SYM denotes +
T5111 19797-19801 NNS denotes mice
T5116 19801-19803 NN denotes ,
T5117 19803-19807 RB denotes even
T5119 19808-19814 IN denotes though
T5120 19815-19822 JJ denotes similar
T5121 19823-19830 NNS denotes amounts
T5122 19831-19833 IN denotes of
T5123 19834-19844 NN denotes fibroblast
T5125 19845-19851 NN denotes colony
T5127 19851-19852 HYPH denotes -
T5126 19852-19859 VBG denotes forming
T5124 19860-19865 NNS denotes units
T5128 19866-19867 -LRB- denotes (
T5129 19867-19870 NN denotes CFU
T5131 19870-19871 HYPH denotes -
T5130 19871-19872 NN denotes F
T5132 19872-19873 -RRB- denotes )
T5133 19874-19878 VBD denotes were
T5118 19879-19887 VBN denotes observed
T5134 19888-19889 -LRB- denotes (
T5136 19889-19895 NN denotes Figure
T5135 19896-19898 NN denotes 6A
T5137 19899-19902 CC denotes and
T5138 19903-19914 JJ denotes unpublished
T5139 19915-19919 NNS denotes data
T5140 19919-19920 -RRB- denotes )
T5141 19920-19921 . denotes .
T5142 19921-20172 sentence denotes RT-PCR analysis of molecular markers of osteoblast differentiation (Dataset S2) revealed similar increases over time in RNA from Sam68+/+ and Sam68−/− mice, although type I collagen appeared to be up-regulated at both timepoints in the Sam68−/− mice.
T5143 19922-19924 NN denotes RT
T5145 19924-19925 HYPH denotes -
T5144 19925-19928 NN denotes PCR
T5146 19929-19937 NN denotes analysis
T5148 19938-19940 IN denotes of
T5149 19941-19950 JJ denotes molecular
T5150 19951-19958 NNS denotes markers
T5151 19959-19961 IN denotes of
T5152 19962-19972 NN denotes osteoblast
T5153 19973-19988 NN denotes differentiation
T5154 19989-19990 -LRB- denotes (
T5156 19990-19997 NN denotes Dataset
T5155 19998-20000 NN denotes S2
T5157 20000-20001 -RRB- denotes )
T5147 20002-20010 VBD denotes revealed
T5158 20011-20018 JJ denotes similar
T5159 20019-20028 NNS denotes increases
T5160 20029-20033 IN denotes over
T5161 20034-20038 NN denotes time
T5162 20039-20041 IN denotes in
T5163 20042-20045 NN denotes RNA
T5164 20046-20050 IN denotes from
T5165 20051-20056 NN denotes Sam68
T5167 20056-20057 SYM denotes +
T5168 20057-20058 HYPH denotes /
T5169 20058-20059 SYM denotes +
T5170 20060-20063 CC denotes and
T5171 20064-20069 NN denotes Sam68
T5172 20069-20070 SYM denotes −
T5173 20070-20071 HYPH denotes /
T5174 20071-20072 SYM denotes −
T5166 20073-20077 NNS denotes mice
T5175 20077-20079 , denotes ,
T5176 20079-20087 IN denotes although
T5178 20088-20092 NN denotes type
T5180 20093-20094 CD denotes I
T5179 20095-20103 NN denotes collagen
T5177 20104-20112 VBD denotes appeared
T5181 20113-20115 TO denotes to
T5183 20116-20118 VB denotes be
T5184 20119-20121 RB denotes up
T5185 20121-20122 HYPH denotes -
T5182 20122-20131 VBN denotes regulated
T5186 20132-20134 IN denotes at
T5187 20135-20139 DT denotes both
T5188 20140-20150 NNS denotes timepoints
T5189 20151-20153 IN denotes in
T5190 20154-20157 DT denotes the
T5192 20158-20163 NN denotes Sam68
T5193 20163-20164 SYM denotes −
T5194 20164-20165 HYPH denotes /
T5195 20165-20166 SYM denotes −
T5191 20167-20171 NNS denotes mice
T5196 20171-20172 . denotes .
T5197 20172-20452 sentence denotes Short-term cultures of mature osteoclasts released from the crushed long bones of Sam68+/+ and Sam68−/− mice stained equally well for TRAP and excavated approximately equal numbers of pits of equal size in dentin slices, as visualized by scanning electron microscopy (Figure 6B).
T5198 20173-20178 JJ denotes Short
T5200 20178-20179 HYPH denotes -
T5199 20179-20183 NN denotes term
T5201 20184-20192 NNS denotes cultures
T5203 20193-20195 IN denotes of
T5204 20196-20202 JJ denotes mature
T5205 20203-20214 NNS denotes osteoclasts
T5206 20215-20223 VBN denotes released
T5207 20224-20228 IN denotes from
T5208 20229-20232 DT denotes the
T5210 20233-20240 JJ denotes crushed
T5211 20241-20245 JJ denotes long
T5209 20246-20251 NNS denotes bones
T5212 20252-20254 IN denotes of
T5213 20255-20260 NN denotes Sam68
T5215 20260-20261 SYM denotes +
T5216 20261-20262 HYPH denotes /
T5217 20262-20263 SYM denotes +
T5218 20264-20267 CC denotes and
T5219 20268-20273 NN denotes Sam68
T5220 20273-20274 SYM denotes −
T5221 20274-20275 HYPH denotes /
T5222 20275-20276 SYM denotes −
T5214 20277-20281 NNS denotes mice
T5202 20282-20289 VBD denotes stained
T5223 20290-20297 RB denotes equally
T5224 20298-20302 RB denotes well
T5225 20303-20306 IN denotes for
T5226 20307-20311 NN denotes TRAP
T5227 20312-20315 CC denotes and
T5228 20316-20325 VBD denotes excavated
T5229 20326-20339 RB denotes approximately
T5230 20340-20345 JJ denotes equal
T5231 20346-20353 NNS denotes numbers
T5232 20354-20356 IN denotes of
T5233 20357-20361 NNS denotes pits
T5234 20362-20364 IN denotes of
T5235 20365-20370 JJ denotes equal
T5236 20371-20375 NN denotes size
T5237 20376-20378 IN denotes in
T5238 20379-20385 NN denotes dentin
T5239 20386-20392 NNS denotes slices
T5240 20392-20394 , denotes ,
T5241 20394-20396 IN denotes as
T5242 20397-20407 VBN denotes visualized
T5243 20408-20410 IN denotes by
T5244 20411-20419 VBG denotes scanning
T5245 20420-20428 NN denotes electron
T5246 20429-20439 NN denotes microscopy
T5247 20440-20441 -LRB- denotes (
T5249 20441-20447 NN denotes Figure
T5248 20448-20450 NN denotes 6B
T5250 20450-20451 -RRB- denotes )
T5251 20451-20452 . denotes .
T5252 20452-20579 sentence denotes These findings suggest that the osteoclasts may not be the primary defect in Sam68−/− mice, as they are in Src null mice [48].
T5253 20453-20458 DT denotes These
T5254 20459-20467 NNS denotes findings
T5255 20468-20475 VBP denotes suggest
T5256 20476-20480 IN denotes that
T5258 20481-20484 DT denotes the
T5259 20485-20496 NNS denotes osteoclasts
T5260 20497-20500 MD denotes may
T5261 20501-20504 RB denotes not
T5257 20505-20507 VB denotes be
T5262 20508-20511 DT denotes the
T5264 20512-20519 JJ denotes primary
T5263 20520-20526 NN denotes defect
T5265 20527-20529 IN denotes in
T5266 20530-20535 NN denotes Sam68
T5268 20535-20536 SYM denotes −
T5269 20536-20537 HYPH denotes /
T5270 20537-20538 SYM denotes −
T5267 20539-20543 NNS denotes mice
T5271 20543-20545 , denotes ,
T5272 20545-20547 IN denotes as
T5274 20548-20552 PRP denotes they
T5273 20553-20556 VBP denotes are
T5275 20557-20559 IN denotes in
T5276 20560-20563 NN denotes Src
T5278 20564-20568 JJ denotes null
T5277 20569-20573 NNS denotes mice
T5279 20574-20575 -LRB- denotes [
T5280 20575-20577 CD denotes 48
T5281 20577-20578 -RRB- denotes ]
T5282 20578-20579 . denotes .
T5689 21645-21648 DT denotes The
T5690 21649-21656 NN denotes Absence
T5692 21657-21659 IN denotes of
T5693 21660-21665 NN denotes Sam68
T5691 21666-21674 VBZ denotes Prevents
T5694 21675-21684 NN denotes Adipocyte
T5695 21685-21700 NN denotes Differentiation
T5696 21701-21704 CC denotes and
T5697 21705-21713 VBZ denotes Promotes
T5698 21714-21724 NN denotes Osteoblast
T5699 21725-21740 NN denotes Differentiation
T5700 21740-21951 sentence denotes It is well recognized that bone marrow stromal cells give rise to both osteoblasts and adipocytes and that age-related bone loss is accompanied by an increase in differentiation down the adipocyte lineage [22].
T5701 21741-21743 PRP denotes It
T5703 21744-21746 VBZ denotes is
T5704 21747-21751 RB denotes well
T5702 21752-21762 VBN denotes recognized
T5705 21763-21767 IN denotes that
T5707 21768-21772 NN denotes bone
T5708 21773-21779 NN denotes marrow
T5710 21780-21787 JJ denotes stromal
T5709 21788-21793 NNS denotes cells
T5706 21794-21798 VBP denotes give
T5711 21799-21803 NN denotes rise
T5712 21804-21806 IN denotes to
T5713 21807-21811 DT denotes both
T5714 21812-21823 NNS denotes osteoblasts
T5715 21824-21827 CC denotes and
T5716 21828-21838 NNS denotes adipocytes
T5717 21839-21842 CC denotes and
T5718 21843-21847 IN denotes that
T5720 21848-21851 NN denotes age
T5722 21851-21852 HYPH denotes -
T5721 21852-21859 VBN denotes related
T5724 21860-21864 NN denotes bone
T5723 21865-21869 NN denotes loss
T5725 21870-21872 VBZ denotes is
T5719 21873-21884 VBN denotes accompanied
T5726 21885-21887 IN denotes by
T5727 21888-21890 DT denotes an
T5728 21891-21899 NN denotes increase
T5729 21900-21902 IN denotes in
T5730 21903-21918 NN denotes differentiation
T5731 21919-21923 IN denotes down
T5732 21924-21927 DT denotes the
T5734 21928-21937 NN denotes adipocyte
T5733 21938-21945 NN denotes lineage
T5735 21946-21947 -LRB- denotes [
T5736 21947-21949 CD denotes 22
T5737 21949-21950 -RRB- denotes ]
T5738 21950-21951 . denotes .
T5739 21951-22095 sentence denotes Therefore, the loss of Sam68 could influence the bone marrow stromal cells to differentiate along the osteogenic versus the adipogenic pathway.
T5740 21952-21961 RB denotes Therefore
T5742 21961-21963 , denotes ,
T5743 21963-21966 DT denotes the
T5744 21967-21971 NN denotes loss
T5745 21972-21974 IN denotes of
T5746 21975-21980 NN denotes Sam68
T5747 21981-21986 MD denotes could
T5741 21987-21996 VB denotes influence
T5748 21997-22000 DT denotes the
T5750 22001-22005 NN denotes bone
T5751 22006-22012 NN denotes marrow
T5752 22013-22020 JJ denotes stromal
T5749 22021-22026 NNS denotes cells
T5753 22027-22029 TO denotes to
T5754 22030-22043 VB denotes differentiate
T5755 22044-22049 IN denotes along
T5756 22050-22053 DT denotes the
T5757 22054-22064 JJ denotes osteogenic
T5758 22065-22071 CC denotes versus
T5759 22072-22075 DT denotes the
T5760 22076-22086 JJ denotes adipogenic
T5761 22087-22094 NN denotes pathway
T5762 22094-22095 . denotes .
T5763 22095-22329 sentence denotes Alternatively, the loss of Sam68 could indirectly regulate bone mass as a general disturbance of neuroendocrine control as was shown when leptin and the sympathetic nervous system axis were shown to negatively regulate bone mass [8].
T5764 22096-22109 RB denotes Alternatively
T5766 22109-22111 , denotes ,
T5767 22111-22114 DT denotes the
T5768 22115-22119 NN denotes loss
T5769 22120-22122 IN denotes of
T5770 22123-22128 NN denotes Sam68
T5771 22129-22134 MD denotes could
T5772 22135-22145 RB denotes indirectly
T5765 22146-22154 VB denotes regulate
T5773 22155-22159 NN denotes bone
T5774 22160-22164 NN denotes mass
T5775 22165-22167 IN denotes as
T5776 22168-22169 DT denotes a
T5778 22170-22177 JJ denotes general
T5777 22178-22189 NN denotes disturbance
T5779 22190-22192 IN denotes of
T5780 22193-22207 JJ denotes neuroendocrine
T5781 22208-22215 NN denotes control
T5782 22216-22218 IN denotes as
T5784 22219-22222 VBD denotes was
T5783 22223-22228 VBN denotes shown
T5785 22229-22233 WRB denotes when
T5787 22234-22240 NN denotes leptin
T5788 22241-22244 CC denotes and
T5789 22245-22248 DT denotes the
T5791 22249-22260 JJ denotes sympathetic
T5792 22261-22268 JJ denotes nervous
T5793 22269-22275 NN denotes system
T5790 22276-22280 NN denotes axis
T5794 22281-22285 VBD denotes were
T5786 22286-22291 VBN denotes shown
T5795 22292-22294 TO denotes to
T5797 22295-22305 RB denotes negatively
T5796 22306-22314 VB denotes regulate
T5798 22315-22319 NN denotes bone
T5799 22320-22324 NN denotes mass
T5800 22325-22326 -LRB- denotes [
T5801 22326-22327 CD denotes 8
T5802 22327-22328 -RRB- denotes ]
T5803 22328-22329 . denotes .
T5804 22329-22479 sentence denotes To confirm a role for Sam68 in the regulation of adipocyte differentiation, we isolated primary MEFs from 14.5-day-old Sam68+/+ and Sam68−/− embryos.
T5805 22330-22332 TO denotes To
T5806 22333-22340 VB denotes confirm
T5808 22341-22342 DT denotes a
T5809 22343-22347 NN denotes role
T5810 22348-22351 IN denotes for
T5811 22352-22357 NN denotes Sam68
T5812 22358-22360 IN denotes in
T5813 22361-22364 DT denotes the
T5814 22365-22375 NN denotes regulation
T5815 22376-22378 IN denotes of
T5816 22379-22388 NN denotes adipocyte
T5817 22389-22404 NN denotes differentiation
T5818 22404-22406 , denotes ,
T5819 22406-22408 PRP denotes we
T5807 22409-22417 VBD denotes isolated
T5820 22418-22425 JJ denotes primary
T5821 22426-22430 NNS denotes MEFs
T5822 22431-22435 IN denotes from
T5823 22436-22440 CD denotes 14.5
T5825 22440-22441 HYPH denotes -
T5824 22441-22444 NN denotes day
T5827 22444-22445 HYPH denotes -
T5826 22445-22448 JJ denotes old
T5829 22449-22454 NN denotes Sam68
T5830 22454-22455 SYM denotes +
T5831 22455-22456 HYPH denotes /
T5832 22456-22457 SYM denotes +
T5833 22458-22461 CC denotes and
T5834 22462-22467 NN denotes Sam68
T5835 22467-22468 SYM denotes −
T5836 22468-22469 HYPH denotes /
T5837 22469-22470 SYM denotes −
T5828 22471-22478 NNS denotes embryos
T5838 22478-22479 . denotes .
T5839 22479-22621 sentence denotes The primary Sam68−/− MEFs were differentiated into adipocytes in vitro in culture medium containing 5 μM pioglitazone to induce adipogenesis.
T5840 22480-22483 DT denotes The
T5842 22484-22491 JJ denotes primary
T5843 22492-22497 NN denotes Sam68
T5844 22497-22498 SYM denotes −
T5845 22498-22499 HYPH denotes /
T5846 22499-22500 SYM denotes −
T5841 22501-22505 NNS denotes MEFs
T5848 22506-22510 VBD denotes were
T5847 22511-22525 VBN denotes differentiated
T5849 22526-22530 IN denotes into
T5850 22531-22541 NNS denotes adipocytes
T5851 22542-22544 FW denotes in
T5852 22545-22550 FW denotes vitro
T5853 22551-22553 IN denotes in
T5854 22554-22561 NN denotes culture
T5855 22562-22568 NN denotes medium
T5856 22569-22579 VBG denotes containing
T5857 22580-22581 CD denotes 5
T5858 22582-22584 NN denotes μM
T5859 22585-22597 NN denotes pioglitazone
T5860 22598-22600 TO denotes to
T5861 22601-22607 VB denotes induce
T5862 22608-22620 NN denotes adipogenesis
T5863 22620-22621 . denotes .
T5864 22621-22704 sentence denotes Cells at days 0, 4, 6, and 12 were stained with Oil red O to monitor adipogenesis.
T5865 22622-22627 NNS denotes Cells
T5867 22628-22630 IN denotes at
T5868 22631-22635 NNS denotes days
T5869 22636-22637 CD denotes 0
T5870 22637-22639 , denotes ,
T5871 22639-22640 CD denotes 4
T5872 22640-22642 , denotes ,
T5873 22642-22643 CD denotes 6
T5874 22643-22645 , denotes ,
T5875 22645-22648 CC denotes and
T5876 22649-22651 CD denotes 12
T5877 22652-22656 VBD denotes were
T5866 22657-22664 VBN denotes stained
T5878 22665-22669 IN denotes with
T5879 22670-22673 NN denotes Oil
T5881 22674-22677 NN denotes red
T5880 22678-22679 NN denotes O
T5882 22680-22682 TO denotes to
T5883 22683-22690 VB denotes monitor
T5884 22691-22703 NN denotes adipogenesis
T5885 22703-22704 . denotes .
T5886 22704-22890 sentence denotes Adipogenesis was more pronounced in the wild-type MEFs cultures than in the Sam68−/− MEFs, consistent with the positive role of endogenous Sam68 in adipocyte differentiation (Figure 7).
T5887 22705-22717 NN denotes Adipogenesis
T5888 22718-22721 VBD denotes was
T5889 22722-22726 RBR denotes more
T5890 22727-22737 JJ denotes pronounced
T5891 22738-22740 IN denotes in
T5892 22741-22744 DT denotes the
T5894 22745-22749 JJ denotes wild
T5896 22749-22750 HYPH denotes -
T5895 22750-22754 NN denotes type
T5897 22755-22759 NNS denotes MEFs
T5893 22760-22768 NNS denotes cultures
T5898 22769-22773 IN denotes than
T5899 22774-22776 IN denotes in
T5900 22777-22780 DT denotes the
T5902 22781-22786 NN denotes Sam68
T5903 22786-22787 SYM denotes −
T5904 22787-22788 HYPH denotes /
T5905 22788-22789 SYM denotes −
T5901 22790-22794 NNS denotes MEFs
T5906 22794-22796 , denotes ,
T5907 22796-22806 JJ denotes consistent
T5908 22807-22811 IN denotes with
T5909 22812-22815 DT denotes the
T5911 22816-22824 JJ denotes positive
T5910 22825-22829 NN denotes role
T5912 22830-22832 IN denotes of
T5913 22833-22843 JJ denotes endogenous
T5914 22844-22849 NN denotes Sam68
T5915 22850-22852 IN denotes in
T5916 22853-22862 NN denotes adipocyte
T5917 22863-22878 NN denotes differentiation
T5918 22879-22880 -LRB- denotes (
T5919 22880-22886 NN denotes Figure
T5920 22887-22888 CD denotes 7
T5921 22888-22889 -RRB- denotes )
T5922 22889-22890 . denotes .
T5923 22890-23111 sentence denotes The expression of key transcription factors including the PPARγ and KLF5 was impaired in Sam68−/− differentiated MEFs compared with Sam68+/+ MEFs, consistent with impaired adipogenesis in the absence of Sam68 (Figure 7).
T5924 22891-22894 DT denotes The
T5925 22895-22905 NN denotes expression
T5927 22906-22908 IN denotes of
T5928 22909-22912 JJ denotes key
T5930 22913-22926 NN denotes transcription
T5929 22927-22934 NNS denotes factors
T5931 22935-22944 VBG denotes including
T5932 22945-22948 DT denotes the
T5933 22949-22954 NN denotes PPARγ
T5934 22955-22958 CC denotes and
T5935 22959-22963 NN denotes KLF5
T5936 22964-22967 VBD denotes was
T5926 22968-22976 VBN denotes impaired
T5937 22977-22979 IN denotes in
T5938 22980-22985 NN denotes Sam68
T5940 22985-22986 SYM denotes −
T5941 22986-22987 HYPH denotes /
T5942 22987-22988 SYM denotes −
T5943 22989-23003 VBN denotes differentiated
T5939 23004-23008 NNS denotes MEFs
T5944 23009-23017 VBN denotes compared
T5945 23018-23022 IN denotes with
T5946 23023-23028 NN denotes Sam68
T5948 23028-23029 SYM denotes +
T5949 23029-23030 HYPH denotes /
T5950 23030-23031 SYM denotes +
T5947 23032-23036 NNS denotes MEFs
T5951 23036-23038 , denotes ,
T5952 23038-23048 JJ denotes consistent
T5953 23049-23053 IN denotes with
T5954 23054-23062 VBN denotes impaired
T5955 23063-23075 NN denotes adipogenesis
T5956 23076-23078 IN denotes in
T5957 23079-23082 DT denotes the
T5958 23083-23090 NN denotes absence
T5959 23091-23093 IN denotes of
T5960 23094-23099 NN denotes Sam68
T5961 23100-23101 -LRB- denotes (
T5962 23101-23107 NN denotes Figure
T5963 23108-23109 CD denotes 7
T5964 23109-23110 -RRB- denotes )
T5965 23110-23111 . denotes .
T5966 23111-23319 sentence denotes These data, together with data confirming a lean phenotype in Sam68−/− mice (N. Torabi and S. Richard, unpublished data), support the hypothesis that Sam68 modulates the differentiation of mesenchymal cells.
T5967 23112-23117 DT denotes These
T5968 23118-23122 NNS denotes data
T5970 23122-23124 , denotes ,
T5971 23124-23132 RB denotes together
T5972 23133-23137 IN denotes with
T5973 23138-23142 NNS denotes data
T5974 23143-23153 VBG denotes confirming
T5975 23154-23155 DT denotes a
T5977 23156-23160 JJ denotes lean
T5976 23161-23170 NN denotes phenotype
T5978 23171-23173 IN denotes in
T5979 23174-23179 NN denotes Sam68
T5981 23179-23180 SYM denotes −
T5982 23180-23181 HYPH denotes /
T5983 23181-23182 SYM denotes −
T5980 23183-23187 NNS denotes mice
T5984 23188-23189 -LRB- denotes (
T5985 23189-23191 NNP denotes N.
T5986 23192-23198 NNP denotes Torabi
T5987 23199-23202 CC denotes and
T5988 23203-23205 NNP denotes S.
T5989 23206-23213 NNP denotes Richard
T5990 23213-23215 , denotes ,
T5991 23215-23226 JJ denotes unpublished
T5992 23227-23231 NNS denotes data
T5993 23231-23232 -RRB- denotes )
T5994 23232-23234 , denotes ,
T5969 23234-23241 VBP denotes support
T5995 23242-23245 DT denotes the
T5996 23246-23256 NN denotes hypothesis
T5997 23257-23261 IN denotes that
T5999 23262-23267 NN denotes Sam68
T5998 23268-23277 VBZ denotes modulates
T6000 23278-23281 DT denotes the
T6001 23282-23297 NN denotes differentiation
T6002 23298-23300 IN denotes of
T6003 23301-23312 JJ denotes mesenchymal
T6004 23313-23318 NNS denotes cells
T6005 23318-23319 . denotes .
T6006 23319-24385 sentence denotes Figure 7 Ex Vivo Adipogenesis Analysis of Sam68−/− Mouse Embryonic Fibroblasts MEFs were isolated from mouse embryos at embryonic day 14.5. Equal number of MEFs from Sam68+/+ and Sam68−/− was plated on glass cover slips in 24 well-plates. Adipocyte differentiation was carried out at indicated times by the addition of complete media containing the pioglitazone. (A) Cultures were fixed in 4% paraformaldehyde and stained with Oil Red O to detect the fat droplets stored in adipocytes and photographed (top). The cell images were magnified ×10 and ×20 as indicated. (B) RT-PCR was carried out on total cellular RNA isolated after differentiation of the MEFs for day 0, 2, 4, 6, and 12. The DNA fragments were visualized on agarose gels stained with ethidium bromide. The expression of adipogenic markers C/EBPβ, C/EBPδ, PPARα, and KLF5 was examined as well as the expression of controls including Sam68, β-actin, and GAPDH. To further examine this phenotype in a cell autonomous system, we chose the embryonic mesenchymal multipotential progenitor cells C3H10T1/2.
T6007 24245-24247 TO denotes To
T6009 24248-24255 RB denotes further
T6008 24256-24263 VB denotes examine
T6011 24264-24268 DT denotes this
T6012 24269-24278 NN denotes phenotype
T6013 24279-24281 IN denotes in
T6014 24282-24283 DT denotes a
T6016 24284-24288 NN denotes cell
T6017 24289-24299 JJ denotes autonomous
T6015 24300-24306 NN denotes system
T6018 24306-24308 , denotes ,
T6019 24308-24310 PRP denotes we
T6010 24311-24316 VBD denotes chose
T6020 24317-24320 DT denotes the
T6022 24321-24330 JJ denotes embryonic
T6023 24331-24342 JJ denotes mesenchymal
T6024 24343-24357 JJ denotes multipotential
T6025 24358-24368 NN denotes progenitor
T6021 24369-24374 NNS denotes cells
T6026 24375-24382 NN denotes C3H10T1
T6027 24382-24383 HYPH denotes /
T6028 24383-24384 CD denotes 2
T6029 24384-24385 . denotes .
T6030 24385-24544 sentence denotes The addition of BMP-2 induced osteoblast differentiation, as evidenced by an approximately 400-fold increase in expression of the osteocalcin (OCN) gene [51].
T6031 24386-24389 DT denotes The
T6032 24390-24398 NN denotes addition
T6034 24399-24401 IN denotes of
T6035 24402-24405 NN denotes BMP
T6036 24405-24406 HYPH denotes -
T6037 24406-24407 CD denotes 2
T6033 24408-24415 VBD denotes induced
T6038 24416-24426 NN denotes osteoblast
T6039 24427-24442 NN denotes differentiation
T6040 24442-24444 , denotes ,
T6041 24444-24446 IN denotes as
T6042 24447-24456 VBN denotes evidenced
T6043 24457-24459 IN denotes by
T6044 24460-24462 DT denotes an
T6046 24463-24476 RB denotes approximately
T6047 24477-24485 JJ denotes 400-fold
T6045 24486-24494 NN denotes increase
T6048 24495-24497 IN denotes in
T6049 24498-24508 NN denotes expression
T6050 24509-24511 IN denotes of
T6051 24512-24515 DT denotes the
T6053 24516-24527 NN denotes osteocalcin
T6054 24528-24529 -LRB- denotes (
T6055 24529-24532 NN denotes OCN
T6056 24532-24533 -RRB- denotes )
T6052 24534-24538 NN denotes gene
T6057 24539-24540 -LRB- denotes [
T6058 24540-24542 CD denotes 51
T6059 24542-24543 -RRB- denotes ]
T6060 24543-24544 . denotes .
T6061 24544-24726 sentence denotes Populations of C3H10T1/2 cells stably transfected with either pSuper-retro (control) and pSuper-retro harboring a short hairpin against Sam68 (Sam68sh) were selected with puromycin.
T6062 24545-24556 NNS denotes Populations
T6064 24557-24559 IN denotes of
T6065 24560-24567 NN denotes C3H10T1
T6067 24567-24568 HYPH denotes /
T6068 24568-24569 CD denotes 2
T6066 24570-24575 NNS denotes cells
T6069 24576-24582 RB denotes stably
T6070 24583-24594 VBN denotes transfected
T6071 24595-24599 IN denotes with
T6072 24600-24606 CC denotes either
T6074 24607-24613 NN denotes pSuper
T6075 24613-24614 HYPH denotes -
T6073 24614-24619 NN denotes retro
T6076 24620-24621 -LRB- denotes (
T6077 24621-24628 NN denotes control
T6078 24628-24629 -RRB- denotes )
T6079 24630-24633 CC denotes and
T6080 24634-24640 NN denotes pSuper
T6082 24640-24641 HYPH denotes -
T6081 24641-24646 NN denotes retro
T6083 24647-24656 VBG denotes harboring
T6084 24657-24658 DT denotes a
T6086 24659-24664 JJ denotes short
T6085 24665-24672 NN denotes hairpin
T6087 24673-24680 IN denotes against
T6088 24681-24686 NN denotes Sam68
T6089 24687-24688 -LRB- denotes (
T6090 24688-24695 NN denotes Sam68sh
T6091 24695-24696 -RRB- denotes )
T6092 24697-24701 VBD denotes were
T6063 24702-24710 VBN denotes selected
T6093 24711-24715 IN denotes with
T6094 24716-24725 NN denotes puromycin
T6095 24725-24726 . denotes .
T6096 24726-24867 sentence denotes The expression of Sam68 was reduced by approximately 80% as evidenced by immunoblot analyses using β-actin as a loading control (Figure 8A).
T6097 24727-24730 DT denotes The
T6098 24731-24741 NN denotes expression
T6100 24742-24744 IN denotes of
T6101 24745-24750 NN denotes Sam68
T6102 24751-24754 VBD denotes was
T6099 24755-24762 VBN denotes reduced
T6103 24763-24765 IN denotes by
T6104 24766-24779 RB denotes approximately
T6105 24780-24782 CD denotes 80
T6106 24782-24783 NN denotes %
T6107 24784-24786 IN denotes as
T6108 24787-24796 VBN denotes evidenced
T6109 24797-24799 IN denotes by
T6110 24800-24810 NN denotes immunoblot
T6111 24811-24819 NNS denotes analyses
T6112 24820-24825 VBG denotes using
T6113 24826-24827 NN denotes β
T6115 24827-24828 HYPH denotes -
T6114 24828-24833 NN denotes actin
T6116 24834-24836 IN denotes as
T6117 24837-24838 DT denotes a
T6119 24839-24846 NN denotes loading
T6118 24847-24854 NN denotes control
T6120 24855-24856 -LRB- denotes (
T6122 24856-24862 NN denotes Figure
T6121 24863-24865 NN denotes 8A
T6123 24865-24866 -RRB- denotes )
T6124 24866-24867 . denotes .
T6125 24867-25012 sentence denotes Osteoblast differentiation was induced in cultures expressing pSuper-retro or pSuper-retro Sam68sh by addition of BMP-2 to the culture medium. .
T6126 24868-24878 NN denotes Osteoblast
T6127 24879-24894 NN denotes differentiation
T6129 24895-24898 VBD denotes was
T6128 24899-24906 VBN denotes induced
T6130 24907-24909 IN denotes in
T6131 24910-24918 NNS denotes cultures
T6132 24919-24929 VBG denotes expressing
T6133 24930-24936 NN denotes pSuper
T6135 24936-24937 HYPH denotes -
T6134 24937-24942 NN denotes retro
T6136 24943-24945 CC denotes or
T6137 24946-24952 NN denotes pSuper
T6139 24952-24953 HYPH denotes -
T6138 24953-24958 NN denotes retro
T6140 24959-24966 NN denotes Sam68sh
T6141 24967-24969 IN denotes by
T6142 24970-24978 NN denotes addition
T6143 24979-24981 IN denotes of
T6144 24982-24985 NN denotes BMP
T6145 24985-24986 HYPH denotes -
T6146 24986-24987 CD denotes 2
T6147 24988-24990 IN denotes to
T6148 24991-24994 DT denotes the
T6150 24995-25002 NN denotes culture
T6149 25003-25009 NN denotes medium
T6151 25009-25010 . denotes .
T6152 25011-25012 . denotes .
T6153 25012-25253 sentence denotes The expression of OCN and β-actin mRNAs was examined by semiquantitative RT-PCR and the Sam68sh-expressing cells displayed a more pronounced osteoblast phenotype compared with control cells, as assessed by the expression of OCN (Figure 8B).
T6154 25013-25016 DT denotes The
T6155 25017-25027 NN denotes expression
T6157 25028-25030 IN denotes of
T6158 25031-25034 NN denotes OCN
T6159 25035-25038 CC denotes and
T6160 25039-25040 NN denotes β
T6162 25040-25041 HYPH denotes -
T6161 25041-25046 NN denotes actin
T6163 25047-25052 NNS denotes mRNAs
T6164 25053-25056 VBD denotes was
T6156 25057-25065 VBN denotes examined
T6165 25066-25068 IN denotes by
T6166 25069-25085 JJ denotes semiquantitative
T6168 25086-25088 NN denotes RT
T6169 25088-25089 HYPH denotes -
T6167 25089-25092 NN denotes PCR
T6170 25093-25096 CC denotes and
T6171 25097-25100 DT denotes the
T6173 25101-25108 NN denotes Sam68sh
T6175 25108-25109 HYPH denotes -
T6174 25109-25119 VBG denotes expressing
T6172 25120-25125 NNS denotes cells
T6176 25126-25135 VBD denotes displayed
T6177 25136-25137 DT denotes a
T6179 25138-25142 RBR denotes more
T6180 25143-25153 JJ denotes pronounced
T6181 25154-25164 NN denotes osteoblast
T6178 25165-25174 NN denotes phenotype
T6182 25175-25183 VBN denotes compared
T6183 25184-25188 IN denotes with
T6184 25189-25196 NN denotes control
T6185 25197-25202 NNS denotes cells
T6186 25202-25204 , denotes ,
T6187 25204-25206 IN denotes as
T6188 25207-25215 VBN denotes assessed
T6189 25216-25218 IN denotes by
T6190 25219-25222 DT denotes the
T6191 25223-25233 NN denotes expression
T6192 25234-25236 IN denotes of
T6193 25237-25240 NN denotes OCN
T6194 25241-25242 -LRB- denotes (
T6196 25242-25248 NN denotes Figure
T6195 25249-25251 NN denotes 8B
T6197 25251-25252 -RRB- denotes )
T6198 25252-25253 . denotes .
T6199 25253-26386 sentence denotes Figure 8 Enhanced Osteogenic Differentiation of the C3HT101/2 Embryonic Cell Line Depleted of Endogenous Sam68 (A) C3HT101/2 cells transfected with an empty vector (pSuper-retro) or a vector containing an shRNA (Sam68 shRNA) were selected with puromycin, and knockdown populations depleted of Sam68 were identified. The reduction in Sam68 protein was analyzed by immunoblotting with anti-Sam68 (AD1) antibody and anti–β-actin antibodies as loading controls. (B) Osteogenic differentiation was carried out with conditioned medium containing BMP-2 for the indicated times. To assess the level of osteogenic differentiation in these cells, expression of late osteoblast marker, osteocalcin (OCN), was analyzed by RT-PCR and compared with β-actin and GAPDH controls. The DNA fragments were visualized by agarose gel stained with ethidium bromide. Given the apparent enhancement of mineralized nodule formation by Sam68−/− bone marrow stromal cells ex vivo and the phenotype observed with short hairpin RNA (shRNA)-treated C3H10T1/2, we stained sections of bone from 4- and 12-month-old mice for evidence of changes in marrow adiposity.
T6200 26098-26103 VBN denotes Given
T6202 26104-26107 DT denotes the
T6204 26108-26116 JJ denotes apparent
T6203 26117-26128 NN denotes enhancement
T6205 26129-26131 IN denotes of
T6206 26132-26143 VBN denotes mineralized
T6207 26144-26150 NN denotes nodule
T6208 26151-26160 NN denotes formation
T6209 26161-26163 IN denotes by
T6210 26164-26169 NN denotes Sam68
T6212 26169-26170 SYM denotes −
T6213 26170-26171 HYPH denotes /
T6214 26171-26172 SYM denotes −
T6215 26173-26177 NN denotes bone
T6216 26178-26184 NN denotes marrow
T6217 26185-26192 JJ denotes stromal
T6211 26193-26198 NNS denotes cells
T6218 26199-26201 FW denotes ex
T6219 26202-26206 FW denotes vivo
T6220 26207-26210 CC denotes and
T6221 26211-26214 DT denotes the
T6222 26215-26224 NN denotes phenotype
T6223 26225-26233 VBN denotes observed
T6224 26234-26238 IN denotes with
T6225 26239-26244 JJ denotes short
T6227 26245-26252 NN denotes hairpin
T6226 26253-26256 NN denotes RNA
T6229 26257-26258 -LRB- denotes (
T6230 26258-26263 NN denotes shRNA
T6231 26263-26264 -RRB- denotes )
T6232 26264-26265 HYPH denotes -
T6228 26265-26272 VBN denotes treated
T6233 26273-26280 NN denotes C3H10T1
T6234 26280-26281 HYPH denotes /
T6235 26281-26282 CD denotes 2
T6236 26282-26284 , denotes ,
T6237 26284-26286 PRP denotes we
T6201 26287-26294 VBD denotes stained
T6238 26295-26303 NNS denotes sections
T6239 26304-26306 IN denotes of
T6240 26307-26311 NN denotes bone
T6241 26312-26316 IN denotes from
T6242 26317-26318 CD denotes 4
T6244 26318-26319 HYPH denotes -
T6245 26320-26323 CC denotes and
T6246 26324-26326 CD denotes 12
T6247 26326-26327 HYPH denotes -
T6243 26327-26332 NN denotes month
T6249 26332-26333 HYPH denotes -
T6248 26333-26336 JJ denotes old
T6250 26337-26341 NNS denotes mice
T6251 26342-26345 IN denotes for
T6252 26346-26354 NN denotes evidence
T6253 26355-26357 IN denotes of
T6254 26358-26365 NNS denotes changes
T6255 26366-26368 IN denotes in
T6256 26369-26375 NN denotes marrow
T6257 26376-26385 NN denotes adiposity
T6258 26385-26386 . denotes .
T6259 26386-26704 sentence denotes Figure 9 shows sections of undecalcified bone from 4- and 12-month-old Sam68+/+ and Sam68−/− mice stained with von Kossa and toluidine blue to show mineralized tissue (Figure 9A, black) and marrow adipocytes (Figure 9B, white) and left unstained to show fluorochrome labeling of the mineralization fronts (Figure 9C).
T6260 26387-26393 NN denotes Figure
T6262 26394-26395 CD denotes 9
T6261 26396-26401 VBZ denotes shows
T6263 26402-26410 NNS denotes sections
T6264 26411-26413 IN denotes of
T6265 26414-26427 JJ denotes undecalcified
T6266 26428-26432 NN denotes bone
T6267 26433-26437 IN denotes from
T6268 26438-26439 CD denotes 4
T6270 26439-26440 HYPH denotes -
T6271 26441-26444 CC denotes and
T6272 26445-26447 CD denotes 12
T6273 26447-26448 HYPH denotes -
T6269 26448-26453 NN denotes month
T6275 26453-26454 HYPH denotes -
T6274 26454-26457 JJ denotes old
T6277 26458-26463 NN denotes Sam68
T6278 26463-26464 SYM denotes +
T6279 26464-26465 HYPH denotes /
T6280 26465-26466 SYM denotes +
T6281 26467-26470 CC denotes and
T6282 26471-26476 NN denotes Sam68
T6283 26476-26477 SYM denotes −
T6284 26477-26478 HYPH denotes /
T6285 26478-26479 SYM denotes −
T6276 26480-26484 NNS denotes mice
T6286 26485-26492 VBN denotes stained
T6287 26493-26497 IN denotes with
T6288 26498-26501 NNP denotes von
T6289 26502-26507 NNP denotes Kossa
T6290 26508-26511 CC denotes and
T6291 26512-26521 NN denotes toluidine
T6292 26522-26526 JJ denotes blue
T6293 26527-26529 TO denotes to
T6294 26530-26534 VB denotes show
T6295 26535-26546 VBN denotes mineralized
T6296 26547-26553 NN denotes tissue
T6297 26554-26555 -LRB- denotes (
T6299 26555-26561 NN denotes Figure
T6298 26562-26564 NN denotes 9A
T6300 26564-26566 , denotes ,
T6301 26566-26571 JJ denotes black
T6302 26571-26572 -RRB- denotes )
T6303 26573-26576 CC denotes and
T6304 26577-26583 NN denotes marrow
T6305 26584-26594 NNS denotes adipocytes
T6306 26595-26596 -LRB- denotes (
T6308 26596-26602 NN denotes Figure
T6307 26603-26605 NN denotes 9B
T6309 26605-26607 , denotes ,
T6310 26607-26612 JJ denotes white
T6311 26612-26613 -RRB- denotes )
T6312 26614-26617 CC denotes and
T6313 26618-26622 VBD denotes left
T6314 26623-26632 JJ denotes unstained
T6315 26633-26635 TO denotes to
T6316 26636-26640 VB denotes show
T6317 26641-26653 NN denotes fluorochrome
T6318 26654-26662 NN denotes labeling
T6319 26663-26665 IN denotes of
T6320 26666-26669 DT denotes the
T6322 26670-26684 NN denotes mineralization
T6321 26685-26691 NNS denotes fronts
T6323 26692-26693 -LRB- denotes (
T6325 26693-26699 NN denotes Figure
T6324 26700-26702 NN denotes 9C
T6326 26702-26703 -RRB- denotes )
T6327 26703-26704 . denotes .
T6328 26704-26957 sentence denotes Twelve-month-old Sam68+/+ mice showed a noticeable decrease in trabecular bone (Figure 9A), which was associated with a significant increase in marrow adiposity (Figure 9B) and with the two fluorochrome labels superimposed upon one another (Figure 9C).
T6329 26705-26711 CD denotes Twelve
T6331 26711-26712 HYPH denotes -
T6330 26712-26717 NN denotes month
T6333 26717-26718 HYPH denotes -
T6332 26718-26721 JJ denotes old
T6335 26722-26727 NN denotes Sam68
T6336 26727-26728 SYM denotes +
T6337 26728-26729 HYPH denotes /
T6338 26729-26730 SYM denotes +
T6334 26731-26735 NNS denotes mice
T6339 26736-26742 VBD denotes showed
T6340 26743-26744 DT denotes a
T6342 26745-26755 JJ denotes noticeable
T6341 26756-26764 NN denotes decrease
T6343 26765-26767 IN denotes in
T6344 26768-26778 JJ denotes trabecular
T6345 26779-26783 NN denotes bone
T6346 26784-26785 -LRB- denotes (
T6348 26785-26791 NN denotes Figure
T6347 26792-26794 NN denotes 9A
T6349 26794-26795 -RRB- denotes )
T6350 26795-26797 , denotes ,
T6351 26797-26802 WDT denotes which
T6353 26803-26806 VBD denotes was
T6352 26807-26817 VBN denotes associated
T6354 26818-26822 IN denotes with
T6355 26823-26824 DT denotes a
T6357 26825-26836 JJ denotes significant
T6356 26837-26845 NN denotes increase
T6358 26846-26848 IN denotes in
T6359 26849-26855 NN denotes marrow
T6360 26856-26865 NN denotes adiposity
T6361 26866-26867 -LRB- denotes (
T6363 26867-26873 NN denotes Figure
T6362 26874-26876 NN denotes 9B
T6364 26876-26877 -RRB- denotes )
T6365 26878-26881 CC denotes and
T6366 26882-26886 IN denotes with
T6367 26887-26890 DT denotes the
T6369 26891-26894 CD denotes two
T6370 26895-26907 NN denotes fluorochrome
T6368 26908-26914 NNS denotes labels
T6371 26915-26927 VBN denotes superimposed
T6372 26928-26932 IN denotes upon
T6373 26933-26936 CD denotes one
T6374 26937-26944 DT denotes another
T6375 26945-26946 -LRB- denotes (
T6377 26946-26952 NN denotes Figure
T6376 26953-26955 NN denotes 9C
T6378 26955-26956 -RRB- denotes )
T6379 26956-26957 . denotes .
T6380 26957-27080 sentence denotes In contrast, the bones of 12-month-old Sam68−/− mice appeared similar to those of the 4-month-old mice of either genotype.
T6381 26958-26960 IN denotes In
T6383 26961-26969 NN denotes contrast
T6384 26969-26971 , denotes ,
T6385 26971-26974 DT denotes the
T6386 26975-26980 NNS denotes bones
T6387 26981-26983 IN denotes of
T6388 26984-26986 CD denotes 12
T6390 26986-26987 HYPH denotes -
T6389 26987-26992 NN denotes month
T6392 26992-26993 HYPH denotes -
T6391 26993-26996 JJ denotes old
T6394 26997-27002 NN denotes Sam68
T6395 27002-27003 SYM denotes −
T6396 27003-27004 HYPH denotes /
T6397 27004-27005 SYM denotes −
T6393 27006-27010 NNS denotes mice
T6382 27011-27019 VBD denotes appeared
T6398 27020-27027 JJ denotes similar
T6399 27028-27030 IN denotes to
T6400 27031-27036 DT denotes those
T6401 27037-27039 IN denotes of
T6402 27040-27043 DT denotes the
T6404 27044-27045 CD denotes 4
T6406 27045-27046 HYPH denotes -
T6405 27046-27051 NN denotes month
T6408 27051-27052 HYPH denotes -
T6407 27052-27055 JJ denotes old
T6403 27056-27060 NNS denotes mice
T6409 27061-27063 IN denotes of
T6410 27064-27070 DT denotes either
T6411 27071-27079 NN denotes genotype
T6412 27079-27080 . denotes .
T6413 27080-27268 sentence denotes These data demonstrate that Sam68 regulates the differentiation of bone marrow mesenchymal cells to promote adipocyte differentiation and inhibit osteoblast differentiation in aging bone.
T6414 27081-27086 DT denotes These
T6415 27087-27091 NNS denotes data
T6416 27092-27103 VBP denotes demonstrate
T6417 27104-27108 IN denotes that
T6419 27109-27114 NN denotes Sam68
T6418 27115-27124 VBZ denotes regulates
T6420 27125-27128 DT denotes the
T6421 27129-27144 NN denotes differentiation
T6422 27145-27147 IN denotes of
T6423 27148-27152 NN denotes bone
T6424 27153-27159 NN denotes marrow
T6426 27160-27171 JJ denotes mesenchymal
T6425 27172-27177 NNS denotes cells
T6427 27178-27180 TO denotes to
T6428 27181-27188 VB denotes promote
T6429 27189-27198 NN denotes adipocyte
T6430 27199-27214 NN denotes differentiation
T6431 27215-27218 CC denotes and
T6432 27219-27226 VB denotes inhibit
T6433 27227-27237 NN denotes osteoblast
T6434 27238-27253 NN denotes differentiation
T6435 27254-27256 IN denotes in
T6436 27257-27262 VBG denotes aging
T6437 27263-27267 NN denotes bone
T6438 27267-27268 . denotes .
T7333 27953-27956 DT denotes The
T7335 27957-27964 JJ denotes present
T7334 27965-27970 NN denotes study
T7336 27971-27979 VBZ denotes provides
T7337 27980-27988 NN denotes evidence
T7338 27989-27993 IN denotes that
T7340 27994-27995 DT denotes a
T7342 27996-28007 JJ denotes physiologic
T7341 28008-28012 NN denotes role
T7343 28013-28015 IN denotes of
T7344 28016-28021 NN denotes Sam68
T7339 28022-28024 VBZ denotes is
T7345 28025-28027 TO denotes to
T7346 28028-28036 VB denotes modulate
T7347 28037-28041 NN denotes bone
T7348 28042-28048 NN denotes marrow
T7350 28049-28060 JJ denotes mesenchymal
T7351 28061-28065 NN denotes stem
T7349 28066-28071 NNS denotes cells
T7352 28071-28072 . denotes .
T7353 28072-28180 sentence denotes Young Sam68−/− mice developed normally and contained similar bone mass compared with wild-type littermates.
T7354 28073-28078 JJ denotes Young
T7356 28079-28084 NN denotes Sam68
T7357 28084-28085 SYM denotes −
T7358 28085-28086 HYPH denotes /
T7359 28086-28087 SYM denotes −
T7355 28088-28092 NNS denotes mice
T7360 28093-28102 VBD denotes developed
T7361 28103-28111 RB denotes normally
T7362 28112-28115 CC denotes and
T7363 28116-28125 VBD denotes contained
T7364 28126-28133 JJ denotes similar
T7366 28134-28138 NN denotes bone
T7365 28139-28143 NN denotes mass
T7367 28144-28152 VBN denotes compared
T7368 28153-28157 IN denotes with
T7369 28158-28162 JJ denotes wild
T7371 28162-28163 HYPH denotes -
T7370 28163-28167 NN denotes type
T7372 28168-28179 NNS denotes littermates
T7373 28179-28180 . denotes .
T7374 28180-28284 sentence denotes Aged (12 months) Sam68−/− mice displayed a high bone mass phenotype compared with Sam68+/+ littermates.
T7375 28181-28185 VBN denotes Aged
T7377 28186-28187 -LRB- denotes (
T7379 28187-28189 CD denotes 12
T7378 28190-28196 NNS denotes months
T7380 28196-28197 -RRB- denotes )
T7381 28198-28203 NN denotes Sam68
T7382 28203-28204 SYM denotes −
T7383 28204-28205 HYPH denotes /
T7384 28205-28206 SYM denotes −
T7376 28207-28211 NNS denotes mice
T7385 28212-28221 VBD denotes displayed
T7386 28222-28223 DT denotes a
T7388 28224-28228 JJ denotes high
T7390 28229-28233 NN denotes bone
T7389 28234-28238 NN denotes mass
T7387 28239-28248 NN denotes phenotype
T7391 28249-28257 VBN denotes compared
T7392 28258-28262 IN denotes with
T7393 28263-28268 NN denotes Sam68
T7395 28268-28269 SYM denotes +
T7396 28269-28270 HYPH denotes /
T7397 28270-28271 SYM denotes +
T7394 28272-28283 NNS denotes littermates
T7398 28283-28284 . denotes .
T7399 28284-28441 sentence denotes The wild-type littermates underwent age-related bone loss that occurs naturally in mammals, while the Sam68−/− animals preserved their bone mass with aging.
T7400 28285-28288 DT denotes The
T7402 28289-28293 JJ denotes wild
T7404 28293-28294 HYPH denotes -
T7403 28294-28298 NN denotes type
T7401 28299-28310 NNS denotes littermates
T7405 28311-28320 VBD denotes underwent
T7406 28321-28324 NN denotes age
T7408 28324-28325 HYPH denotes -
T7407 28325-28332 VBN denotes related
T7410 28333-28337 NN denotes bone
T7409 28338-28342 NN denotes loss
T7411 28343-28347 WDT denotes that
T7412 28348-28354 VBZ denotes occurs
T7413 28355-28364 RB denotes naturally
T7414 28365-28367 IN denotes in
T7415 28368-28375 NNS denotes mammals
T7416 28375-28377 , denotes ,
T7417 28377-28382 IN denotes while
T7419 28383-28386 DT denotes the
T7421 28387-28392 NN denotes Sam68
T7422 28392-28393 SYM denotes −
T7423 28393-28394 HYPH denotes /
T7424 28394-28395 SYM denotes −
T7420 28396-28403 NNS denotes animals
T7418 28404-28413 VBD denotes preserved
T7425 28414-28419 PRP$ denotes their
T7427 28420-28424 NN denotes bone
T7426 28425-28429 NN denotes mass
T7428 28430-28434 IN denotes with
T7429 28435-28440 NN denotes aging
T7430 28440-28441 . denotes .
T7431 28441-28666 sentence denotes The differentiation of bone marrow stem cells isolated from Sam68−/− mice and embryonic mesenchymal multipotential progenitor cells C3H10T1/2 treated with Sam68 shRNA resulted in a more pronounced osteoblast differentiation.
T7432 28442-28445 DT denotes The
T7433 28446-28461 NN denotes differentiation
T7435 28462-28464 IN denotes of
T7436 28465-28469 NN denotes bone
T7437 28470-28476 NN denotes marrow
T7439 28477-28481 NN denotes stem
T7438 28482-28487 NNS denotes cells
T7440 28488-28496 VBN denotes isolated
T7441 28497-28501 IN denotes from
T7442 28502-28507 NN denotes Sam68
T7444 28507-28508 SYM denotes −
T7445 28508-28509 HYPH denotes /
T7446 28509-28510 SYM denotes −
T7443 28511-28515 NNS denotes mice
T7447 28516-28519 CC denotes and
T7448 28520-28529 JJ denotes embryonic
T7450 28530-28541 JJ denotes mesenchymal
T7451 28542-28556 JJ denotes multipotential
T7452 28557-28567 NN denotes progenitor
T7449 28568-28573 NNS denotes cells
T7453 28574-28581 NN denotes C3H10T1
T7454 28581-28582 HYPH denotes /
T7455 28582-28583 CD denotes 2
T7456 28584-28591 VBN denotes treated
T7457 28592-28596 IN denotes with
T7458 28597-28602 NN denotes Sam68
T7459 28603-28608 NN denotes shRNA
T7434 28609-28617 VBD denotes resulted
T7460 28618-28620 IN denotes in
T7461 28621-28622 DT denotes a
T7463 28623-28627 RBR denotes more
T7464 28628-28638 JJ denotes pronounced
T7465 28639-28649 NN denotes osteoblast
T7462 28650-28665 NN denotes differentiation
T7466 28665-28666 . denotes .
T7467 28666-28753 sentence denotes These findings demonstrate that the loss of Sam68 enhances osteoblast differentiation.
T7468 28667-28672 DT denotes These
T7469 28673-28681 NNS denotes findings
T7470 28682-28693 VBP denotes demonstrate
T7471 28694-28698 IN denotes that
T7473 28699-28702 DT denotes the
T7474 28703-28707 NN denotes loss
T7475 28708-28710 IN denotes of
T7476 28711-28716 NN denotes Sam68
T7472 28717-28725 VBZ denotes enhances
T7477 28726-28736 NN denotes osteoblast
T7478 28737-28752 NN denotes differentiation
T7479 28752-28753 . denotes .
T7480 28753-28917 sentence denotes The converse was also true, as MEFs isolated from Sam68−/− animals were impaired in their ability to undergo adipocyte differentiation compared with Sam68+/+ MEFs.
T7481 28754-28757 DT denotes The
T7482 28758-28766 NN denotes converse
T7483 28767-28770 VBD denotes was
T7484 28771-28775 RB denotes also
T7485 28776-28780 JJ denotes true
T7486 28780-28782 , denotes ,
T7487 28782-28784 IN denotes as
T7489 28785-28789 NNS denotes MEFs
T7490 28790-28798 VBN denotes isolated
T7491 28799-28803 IN denotes from
T7492 28804-28809 NN denotes Sam68
T7494 28809-28810 SYM denotes −
T7495 28810-28811 HYPH denotes /
T7496 28811-28812 SYM denotes −
T7493 28813-28820 NNS denotes animals
T7497 28821-28825 VBD denotes were
T7488 28826-28834 VBN denotes impaired
T7498 28835-28837 IN denotes in
T7499 28838-28843 PRP$ denotes their
T7500 28844-28851 NN denotes ability
T7501 28852-28854 TO denotes to
T7502 28855-28862 VB denotes undergo
T7503 28863-28872 NN denotes adipocyte
T7504 28873-28888 NN denotes differentiation
T7505 28889-28897 VBN denotes compared
T7506 28898-28902 IN denotes with
T7507 28903-28908 NN denotes Sam68
T7509 28908-28909 SYM denotes +
T7510 28909-28910 HYPH denotes /
T7511 28910-28911 SYM denotes +
T7508 28912-28916 NNS denotes MEFs
T7512 28916-28917 . denotes .
T7513 28917-29083 sentence denotes These findings suggest that a physiologic role for Sam68 is to regulate the balance between adipogenic and osteogenic differentiation of the bone marrow mesenchymal.
T7514 28918-28923 DT denotes These
T7515 28924-28932 NNS denotes findings
T7516 28933-28940 VBP denotes suggest
T7517 28941-28945 IN denotes that
T7519 28946-28947 DT denotes a
T7521 28948-28959 JJ denotes physiologic
T7520 28960-28964 NN denotes role
T7522 28965-28968 IN denotes for
T7523 28969-28974 NN denotes Sam68
T7518 28975-28977 VBZ denotes is
T7524 28978-28980 TO denotes to
T7525 28981-28989 VB denotes regulate
T7526 28990-28993 DT denotes the
T7527 28994-29001 NN denotes balance
T7528 29002-29009 IN denotes between
T7529 29010-29020 JJ denotes adipogenic
T7531 29021-29024 CC denotes and
T7532 29025-29035 JJ denotes osteogenic
T7530 29036-29051 NN denotes differentiation
T7533 29052-29054 IN denotes of
T7534 29055-29058 DT denotes the
T7536 29059-29063 NN denotes bone
T7535 29064-29070 NN denotes marrow
T7537 29071-29082 JJ denotes mesenchymal
T7538 29082-29083 . denotes .
T7539 29083-29254 sentence denotes Sam68 protein expression was observed throughout the developing mouse embryo, in keeping with previous reports that identified the Sam68 mRNA to be widely expressed [38].
T7540 29084-29089 NN denotes Sam68
T7542 29090-29097 NN denotes protein
T7541 29098-29108 NN denotes expression
T7544 29109-29112 VBD denotes was
T7543 29113-29121 VBN denotes observed
T7545 29122-29132 IN denotes throughout
T7546 29133-29136 DT denotes the
T7548 29137-29147 VBG denotes developing
T7549 29148-29153 NN denotes mouse
T7547 29154-29160 NN denotes embryo
T7550 29160-29162 , denotes ,
T7551 29162-29164 IN denotes in
T7552 29165-29172 VBG denotes keeping
T7553 29173-29177 IN denotes with
T7554 29178-29186 JJ denotes previous
T7555 29187-29194 NNS denotes reports
T7556 29195-29199 WDT denotes that
T7557 29200-29210 VBD denotes identified
T7558 29211-29214 DT denotes the
T7560 29215-29220 NN denotes Sam68
T7559 29221-29225 NN denotes mRNA
T7562 29226-29228 TO denotes to
T7563 29229-29231 VB denotes be
T7564 29232-29238 RB denotes widely
T7561 29239-29248 VBN denotes expressed
T7565 29249-29250 -LRB- denotes [
T7566 29250-29252 CD denotes 38
T7567 29252-29253 -RRB- denotes ]
T7568 29253-29254 . denotes .
T7569 29254-29407 sentence denotes We observe Sam68 staining in the brain, heart, and intestine (see Figure 1) as well as liver, skin, and kidney (unpublished data) of late-stage embryos.
T7570 29255-29257 PRP denotes We
T7571 29258-29265 VBP denotes observe
T7572 29266-29271 NN denotes Sam68
T7573 29272-29280 NN denotes staining
T7574 29281-29283 IN denotes in
T7575 29284-29287 DT denotes the
T7576 29288-29293 NN denotes brain
T7577 29293-29295 , denotes ,
T7578 29295-29300 NN denotes heart
T7579 29300-29302 , denotes ,
T7580 29302-29305 CC denotes and
T7581 29306-29315 NN denotes intestine
T7582 29316-29317 -LRB- denotes (
T7583 29317-29320 VB denotes see
T7584 29321-29327 NN denotes Figure
T7585 29328-29329 CD denotes 1
T7586 29329-29330 -RRB- denotes )
T7587 29331-29333 RB denotes as
T7589 29334-29338 RB denotes well
T7588 29339-29341 IN denotes as
T7590 29342-29347 NN denotes liver
T7591 29347-29349 , denotes ,
T7592 29349-29353 NN denotes skin
T7593 29353-29355 , denotes ,
T7594 29355-29358 CC denotes and
T7595 29359-29365 NN denotes kidney
T7596 29366-29367 -LRB- denotes (
T7598 29367-29378 JJ denotes unpublished
T7597 29379-29383 NNS denotes data
T7599 29383-29384 -RRB- denotes )
T7600 29385-29387 IN denotes of
T7601 29388-29392 JJ denotes late
T7603 29392-29393 HYPH denotes -
T7602 29393-29398 NN denotes stage
T7604 29399-29406 NNS denotes embryos
T7605 29406-29407 . denotes .
T7606 29407-29571 sentence denotes This expression pattern is consistent with previous work that has observed Sam68 in neurons [52] and as a substrate of the intestinal SIK/breast tumor kinase [43].
T7607 29408-29412 DT denotes This
T7609 29413-29423 NN denotes expression
T7608 29424-29431 NN denotes pattern
T7610 29432-29434 VBZ denotes is
T7611 29435-29445 JJ denotes consistent
T7612 29446-29450 IN denotes with
T7613 29451-29459 JJ denotes previous
T7614 29460-29464 NN denotes work
T7615 29465-29469 WDT denotes that
T7617 29470-29473 VBZ denotes has
T7616 29474-29482 VBN denotes observed
T7618 29483-29488 NN denotes Sam68
T7619 29489-29491 IN denotes in
T7620 29492-29499 NNS denotes neurons
T7621 29500-29501 -LRB- denotes [
T7622 29501-29503 CD denotes 52
T7623 29503-29504 -RRB- denotes ]
T7624 29505-29508 CC denotes and
T7625 29509-29511 IN denotes as
T7626 29512-29513 DT denotes a
T7627 29514-29523 NN denotes substrate
T7628 29524-29526 IN denotes of
T7629 29527-29530 DT denotes the
T7631 29531-29541 JJ denotes intestinal
T7632 29542-29545 NN denotes SIK
T7633 29545-29546 HYPH denotes /
T7634 29546-29552 NN denotes breast
T7635 29553-29558 NN denotes tumor
T7630 29559-29565 NN denotes kinase
T7636 29566-29567 -LRB- denotes [
T7637 29567-29569 CD denotes 43
T7638 29569-29570 -RRB- denotes ]
T7639 29570-29571 . denotes .
T7640 29571-29697 sentence denotes The expression of Sam68 was not altered in aging bone marrow stromal cells or in senescencing WI-38 cells (unpublished data).
T7641 29572-29575 DT denotes The
T7642 29576-29586 NN denotes expression
T7644 29587-29589 IN denotes of
T7645 29590-29595 NN denotes Sam68
T7646 29596-29599 VBD denotes was
T7647 29600-29603 RB denotes not
T7643 29604-29611 VBN denotes altered
T7648 29612-29614 IN denotes in
T7649 29615-29620 VBG denotes aging
T7651 29621-29625 NN denotes bone
T7652 29626-29632 NN denotes marrow
T7653 29633-29640 JJ denotes stromal
T7650 29641-29646 NNS denotes cells
T7654 29647-29649 CC denotes or
T7655 29650-29652 IN denotes in
T7656 29653-29665 VBG denotes senescencing
T7658 29666-29668 NN denotes WI
T7659 29668-29669 HYPH denotes -
T7660 29669-29671 CD denotes 38
T7657 29672-29677 NNS denotes cells
T7661 29678-29679 -LRB- denotes (
T7663 29679-29690 JJ denotes unpublished
T7662 29691-29695 NNS denotes data
T7664 29695-29696 -RRB- denotes )
T7665 29696-29697 . denotes .
T7666 29697-29863 sentence denotes The presence of Sam68 in chondrocytes, osteoblasts and osteoclasts in developing cartilage and bone predicted a pivotal role for the protein in skeletal development.
T7667 29698-29701 DT denotes The
T7668 29702-29710 NN denotes presence
T7670 29711-29713 IN denotes of
T7671 29714-29719 NN denotes Sam68
T7672 29720-29722 IN denotes in
T7673 29723-29735 NNS denotes chondrocytes
T7674 29735-29737 , denotes ,
T7675 29737-29748 NNS denotes osteoblasts
T7676 29749-29752 CC denotes and
T7677 29753-29764 NNS denotes osteoclasts
T7678 29765-29767 IN denotes in
T7679 29768-29778 VBG denotes developing
T7680 29779-29788 NN denotes cartilage
T7681 29789-29792 CC denotes and
T7682 29793-29797 NN denotes bone
T7669 29798-29807 VBD denotes predicted
T7683 29808-29809 DT denotes a
T7685 29810-29817 JJ denotes pivotal
T7684 29818-29822 NN denotes role
T7686 29823-29826 IN denotes for
T7687 29827-29830 DT denotes the
T7688 29831-29838 NN denotes protein
T7689 29839-29841 IN denotes in
T7690 29842-29850 JJ denotes skeletal
T7691 29851-29862 NN denotes development
T7692 29862-29863 . denotes .
T7693 29863-30075 sentence denotes The Sam68−/− mice were generated using a traditional targeting approach where the functional KH domain was deleted and approximately one third of the Sam68−/− mice survived to adulthood with no apparent defects.
T7694 29864-29867 DT denotes The
T7696 29868-29873 NN denotes Sam68
T7697 29873-29874 SYM denotes −
T7698 29874-29875 HYPH denotes /
T7699 29875-29876 SYM denotes −
T7695 29877-29881 NNS denotes mice
T7701 29882-29886 VBD denotes were
T7700 29887-29896 VBN denotes generated
T7702 29897-29902 VBG denotes using
T7703 29903-29904 DT denotes a
T7705 29905-29916 JJ denotes traditional
T7706 29917-29926 NN denotes targeting
T7704 29927-29935 NN denotes approach
T7707 29936-29941 WRB denotes where
T7709 29942-29945 DT denotes the
T7711 29946-29956 JJ denotes functional
T7712 29957-29959 NN denotes KH
T7710 29960-29966 NN denotes domain
T7713 29967-29970 VBD denotes was
T7708 29971-29978 VBN denotes deleted
T7714 29979-29982 CC denotes and
T7715 29983-29996 RB denotes approximately
T7716 29997-30000 CD denotes one
T7718 30001-30006 JJ denotes third
T7719 30007-30009 IN denotes of
T7720 30010-30013 DT denotes the
T7722 30014-30019 NN denotes Sam68
T7723 30019-30020 SYM denotes −
T7724 30020-30021 HYPH denotes /
T7725 30021-30022 SYM denotes −
T7721 30023-30027 NNS denotes mice
T7717 30028-30036 VBD denotes survived
T7726 30037-30039 IN denotes to
T7727 30040-30049 NN denotes adulthood
T7728 30050-30054 IN denotes with
T7729 30055-30057 DT denotes no
T7731 30058-30066 JJ denotes apparent
T7730 30067-30074 NNS denotes defects
T7732 30074-30075 . denotes .
T7733 30075-30234 sentence denotes One explanation for this phenomenon is that Sam68 function is sub-served by one or more of its family members during development such as SLM-1 and SLM-2 [47].
T7734 30076-30079 CD denotes One
T7735 30080-30091 NN denotes explanation
T7737 30092-30095 IN denotes for
T7738 30096-30100 DT denotes this
T7739 30101-30111 NN denotes phenomenon
T7736 30112-30114 VBZ denotes is
T7740 30115-30119 IN denotes that
T7742 30120-30125 NN denotes Sam68
T7743 30126-30134 NN denotes function
T7744 30135-30137 VBZ denotes is
T7741 30138-30148 VBN denotes sub-served
T7745 30149-30151 IN denotes by
T7746 30152-30155 CD denotes one
T7747 30156-30158 CC denotes or
T7748 30159-30163 JJR denotes more
T7749 30164-30166 IN denotes of
T7750 30167-30170 PRP$ denotes its
T7752 30171-30177 NN denotes family
T7751 30178-30185 NNS denotes members
T7753 30186-30192 IN denotes during
T7754 30193-30204 NN denotes development
T7755 30205-30209 JJ denotes such
T7756 30210-30212 IN denotes as
T7757 30213-30216 NN denotes SLM
T7758 30216-30217 HYPH denotes -
T7759 30217-30218 CD denotes 1
T7760 30219-30222 CC denotes and
T7761 30223-30226 NN denotes SLM
T7762 30226-30227 HYPH denotes -
T7763 30227-30228 CD denotes 2
T7764 30229-30230 -LRB- denotes [
T7765 30230-30232 CD denotes 47
T7766 30232-30233 -RRB- denotes ]
T7767 30233-30234 . denotes .
T7768 30234-30301 sentence denotes Alternatively, Sam68 is not required during embryonic development.
T7769 30235-30248 RB denotes Alternatively
T7771 30248-30250 , denotes ,
T7772 30250-30255 NN denotes Sam68
T7773 30256-30258 VBZ denotes is
T7774 30259-30262 RB denotes not
T7770 30263-30271 VBN denotes required
T7775 30272-30278 IN denotes during
T7776 30279-30288 JJ denotes embryonic
T7777 30289-30300 NN denotes development
T7778 30300-30301 . denotes .
T7779 30301-30404 sentence denotes However, two thirds of the Sam68−/−, but not the Sam68+/- pups, were killed by their Sam68+/- mothers.
T7780 30302-30309 RB denotes However
T7782 30309-30311 , denotes ,
T7783 30311-30314 CD denotes two
T7784 30315-30321 NNS denotes thirds
T7785 30322-30324 IN denotes of
T7786 30325-30328 DT denotes the
T7787 30329-30334 NN denotes Sam68
T7788 30334-30335 SYM denotes −
T7789 30335-30336 HYPH denotes /
T7790 30336-30337 SYM denotes −
T7791 30337-30339 , denotes ,
T7792 30339-30342 CC denotes but
T7793 30343-30346 RB denotes not
T7794 30347-30350 DT denotes the
T7795 30351-30356 NN denotes Sam68
T7797 30356-30357 SYM denotes +
T7798 30357-30358 HYPH denotes /
T7799 30358-30359 SYM denotes -
T7796 30360-30364 NNS denotes pups
T7800 30364-30366 , denotes ,
T7801 30366-30370 VBD denotes were
T7781 30371-30377 VBN denotes killed
T7802 30378-30380 IN denotes by
T7803 30381-30386 PRP$ denotes their
T7805 30387-30392 NN denotes Sam68
T7806 30392-30393 SYM denotes +
T7807 30393-30394 HYPH denotes /
T7808 30394-30395 SYM denotes -
T7804 30396-30403 NNS denotes mothers
T7809 30403-30404 . denotes .
T7810 30404-30506 sentence denotes These findings suggest that the mothers are able to detect a subtle defect/difference that we cannot.
T7811 30405-30410 DT denotes These
T7812 30411-30419 NNS denotes findings
T7813 30420-30427 VBP denotes suggest
T7814 30428-30432 IN denotes that
T7816 30433-30436 DT denotes the
T7817 30437-30444 NNS denotes mothers
T7815 30445-30448 VBP denotes are
T7818 30449-30453 JJ denotes able
T7819 30454-30456 TO denotes to
T7820 30457-30463 VB denotes detect
T7821 30464-30465 DT denotes a
T7823 30466-30472 JJ denotes subtle
T7824 30473-30479 NN denotes defect
T7825 30479-30480 HYPH denotes /
T7822 30480-30490 NN denotes difference
T7826 30491-30495 WDT denotes that
T7828 30496-30498 PRP denotes we
T7827 30499-30502 MD denotes can
T7829 30502-30505 RB denotes not
T7830 30505-30506 . denotes .
T7831 30506-30578 sentence denotes Adult Sam68−/− mice live a normal life span of approximately two years.
T7832 30507-30512 JJ denotes Adult
T7834 30513-30518 NN denotes Sam68
T7835 30518-30519 SYM denotes −
T7836 30519-30520 HYPH denotes /
T7837 30520-30521 SYM denotes −
T7833 30522-30526 NNS denotes mice
T7838 30527-30531 VBP denotes live
T7839 30532-30533 DT denotes a
T7841 30534-30540 JJ denotes normal
T7842 30541-30545 NN denotes life
T7840 30546-30550 NN denotes span
T7843 30551-30553 IN denotes of
T7844 30554-30567 RB denotes approximately
T7845 30568-30571 CD denotes two
T7846 30572-30577 NNS denotes years
T7847 30577-30578 . denotes .
T7848 30578-30758 sentence denotes The Sam68−/− males were sterile and the Sam68−/− females provided inadequate care to their young, but the pups were not scattered and neglected as observed with FosB−/− mice [53].
T7849 30579-30582 DT denotes The
T7851 30583-30588 NN denotes Sam68
T7852 30588-30589 SYM denotes −
T7853 30589-30590 HYPH denotes /
T7854 30590-30591 SYM denotes −
T7850 30592-30597 NNS denotes males
T7855 30598-30602 VBD denotes were
T7856 30603-30610 JJ denotes sterile
T7857 30611-30614 CC denotes and
T7858 30615-30618 DT denotes the
T7860 30619-30624 NN denotes Sam68
T7861 30624-30625 SYM denotes −
T7862 30625-30626 HYPH denotes /
T7863 30626-30627 SYM denotes −
T7859 30628-30635 NNS denotes females
T7864 30636-30644 VBD denotes provided
T7865 30645-30655 JJ denotes inadequate
T7866 30656-30660 NN denotes care
T7867 30661-30663 IN denotes to
T7868 30664-30669 PRP$ denotes their
T7869 30670-30675 JJ denotes young
T7870 30675-30677 , denotes ,
T7871 30677-30680 CC denotes but
T7872 30681-30684 DT denotes the
T7873 30685-30689 NNS denotes pups
T7875 30690-30694 VBD denotes were
T7876 30695-30698 RB denotes not
T7874 30699-30708 VBN denotes scattered
T7877 30709-30712 CC denotes and
T7878 30713-30722 VBN denotes neglected
T7879 30723-30725 IN denotes as
T7880 30726-30734 VBN denotes observed
T7881 30735-30739 IN denotes with
T7882 30740-30744 NN denotes FosB
T7884 30744-30745 SYM denotes −
T7885 30745-30746 HYPH denotes /
T7886 30746-30747 SYM denotes −
T7883 30748-30752 NNS denotes mice
T7887 30753-30754 -LRB- denotes [
T7888 30754-30756 CD denotes 53
T7889 30756-30757 -RRB- denotes ]
T7890 30757-30758 . denotes .
T7891 30758-30895 sentence denotes Serum levels of estrogen decreased in aging Sam68−/− females as expected; however, the leptin levels decreased in aged Sam68−/− females.
T7892 30759-30764 NN denotes Serum
T7893 30765-30771 NNS denotes levels
T7895 30772-30774 IN denotes of
T7896 30775-30783 NN denotes estrogen
T7894 30784-30793 VBD denotes decreased
T7898 30794-30796 IN denotes in
T7899 30797-30802 VBG denotes aging
T7901 30803-30808 NN denotes Sam68
T7902 30808-30809 SYM denotes −
T7903 30809-30810 HYPH denotes /
T7904 30810-30811 SYM denotes −
T7900 30812-30819 NNS denotes females
T7905 30820-30822 IN denotes as
T7906 30823-30831 VBN denotes expected
T7907 30831-30832 : denotes ;
T7908 30833-30840 RB denotes however
T7909 30840-30842 , denotes ,
T7910 30842-30845 DT denotes the
T7912 30846-30852 NN denotes leptin
T7911 30853-30859 NNS denotes levels
T7897 30860-30869 VBD denotes decreased
T7913 30870-30872 IN denotes in
T7914 30873-30877 JJ denotes aged
T7916 30878-30883 NN denotes Sam68
T7917 30883-30884 SYM denotes −
T7918 30884-30885 HYPH denotes /
T7919 30885-30886 SYM denotes −
T7915 30887-30894 NNS denotes females
T7920 30894-30895 . denotes .
T7921 30895-31006 sentence denotes The aged Sam68−/− females were not obese and actually weighed less than the littermate controls (see Table 2).
T7922 30896-30899 DT denotes The
T7924 30900-30904 JJ denotes aged
T7925 30905-30910 NN denotes Sam68
T7926 30910-30911 SYM denotes −
T7927 30911-30912 HYPH denotes /
T7928 30912-30913 SYM denotes −
T7923 30914-30921 NNS denotes females
T7929 30922-30926 VBD denotes were
T7930 30927-30930 RB denotes not
T7931 30931-30936 JJ denotes obese
T7932 30937-30940 CC denotes and
T7933 30941-30949 RB denotes actually
T7934 30950-30957 VBD denotes weighed
T7935 30958-30962 JJR denotes less
T7936 30963-30967 IN denotes than
T7937 30968-30971 DT denotes the
T7939 30972-30982 NN denotes littermate
T7938 30983-30991 NNS denotes controls
T7940 30992-30993 -LRB- denotes (
T7941 30993-30996 VB denotes see
T7942 30997-31002 NN denotes Table
T7943 31003-31004 CD denotes 2
T7944 31004-31005 -RRB- denotes )
T7945 31005-31006 . denotes .
T7946 31006-31246 sentence denotes Moreover, their appetite was not altered with aging (N. Torabi and S. Richard, unpublished data), suggesting that the observed leptin reduction was not recreating ob/ob-like phenotypes related to weight, appetite and female sterility [54].
T7947 31007-31015 RB denotes Moreover
T7949 31015-31017 , denotes ,
T7950 31017-31022 PRP$ denotes their
T7951 31023-31031 NN denotes appetite
T7952 31032-31035 VBD denotes was
T7953 31036-31039 RB denotes not
T7948 31040-31047 VBN denotes altered
T7954 31048-31052 IN denotes with
T7955 31053-31058 NN denotes aging
T7956 31059-31060 -LRB- denotes (
T7957 31060-31062 NNP denotes N.
T7958 31063-31069 NNP denotes Torabi
T7959 31070-31073 CC denotes and
T7960 31074-31076 NNP denotes S.
T7961 31077-31084 NNP denotes Richard
T7962 31084-31086 , denotes ,
T7963 31086-31097 JJ denotes unpublished
T7964 31098-31102 NNS denotes data
T7965 31102-31103 -RRB- denotes )
T7966 31103-31105 , denotes ,
T7967 31105-31115 VBG denotes suggesting
T7968 31116-31120 IN denotes that
T7970 31121-31124 DT denotes the
T7972 31125-31133 VBN denotes observed
T7973 31134-31140 NN denotes leptin
T7971 31141-31150 NN denotes reduction
T7974 31151-31154 VBD denotes was
T7975 31155-31158 RB denotes not
T7969 31159-31169 VBG denotes recreating
T7976 31170-31172 NN denotes ob
T7978 31172-31173 HYPH denotes /
T7979 31173-31175 NN denotes ob
T7980 31175-31176 HYPH denotes -
T7977 31176-31180 JJ denotes like
T7981 31181-31191 NNS denotes phenotypes
T7982 31192-31199 VBN denotes related
T7983 31200-31202 IN denotes to
T7984 31203-31209 NN denotes weight
T7985 31209-31211 , denotes ,
T7986 31211-31219 NN denotes appetite
T7987 31220-31223 CC denotes and
T7988 31224-31230 JJ denotes female
T7989 31231-31240 NN denotes sterility
T7990 31241-31242 -LRB- denotes [
T7991 31242-31244 CD denotes 54
T7992 31244-31245 -RRB- denotes ]
T7993 31245-31246 . denotes .
T7994 31246-31371 sentence denotes The skeletal phenotyping of cohorts of Sam68+/+ and Sam68−/− mice showed that bone mass was preserved in aged Sam68−/− mice.
T7995 31247-31250 DT denotes The
T7997 31251-31259 JJ denotes skeletal
T7996 31260-31271 NN denotes phenotyping
T7999 31272-31274 IN denotes of
T8000 31275-31282 NNS denotes cohorts
T8001 31283-31285 IN denotes of
T8002 31286-31291 NN denotes Sam68
T8004 31291-31292 SYM denotes +
T8005 31292-31293 HYPH denotes /
T8006 31293-31294 SYM denotes +
T8007 31295-31298 CC denotes and
T8008 31299-31304 NN denotes Sam68
T8009 31304-31305 SYM denotes −
T8010 31305-31306 HYPH denotes /
T8011 31306-31307 SYM denotes −
T8003 31308-31312 NNS denotes mice
T7998 31313-31319 VBD denotes showed
T8012 31320-31324 IN denotes that
T8014 31325-31329 NN denotes bone
T8015 31330-31334 NN denotes mass
T8016 31335-31338 VBD denotes was
T8013 31339-31348 VBN denotes preserved
T8017 31349-31351 IN denotes in
T8018 31352-31356 JJ denotes aged
T8020 31357-31362 NN denotes Sam68
T8021 31362-31363 SYM denotes −
T8022 31363-31364 HYPH denotes /
T8023 31364-31365 SYM denotes −
T8019 31366-31370 NNS denotes mice
T8024 31370-31371 . denotes .
T8025 31371-31504 sentence denotes Traditional histology and histomorphometry suggested that the mechanism involved preservation of osteoblast and osteoclast activity.
T8026 31372-31383 JJ denotes Traditional
T8027 31384-31393 NN denotes histology
T8029 31394-31397 CC denotes and
T8030 31398-31414 NN denotes histomorphometry
T8028 31415-31424 VBD denotes suggested
T8031 31425-31429 IN denotes that
T8033 31430-31433 DT denotes the
T8034 31434-31443 NN denotes mechanism
T8032 31444-31452 VBD denotes involved
T8035 31453-31465 NN denotes preservation
T8036 31466-31468 IN denotes of
T8037 31469-31479 NN denotes osteoblast
T8039 31480-31483 CC denotes and
T8040 31484-31494 NN denotes osteoclast
T8038 31495-31503 NN denotes activity
T8041 31503-31504 DT denotes .
T8042 31504-31662 sentence denotes The documented role of the Sam68 regulatory protein, Src, in osteopetrosis led us to investigate the morphology and activity of Sam68−/− osteoclasts ex vivo.
T8043 31505-31508 DT denotes The
T8045 31509-31519 VBN denotes documented
T8044 31520-31524 NN denotes role
T8047 31525-31527 IN denotes of
T8048 31528-31531 DT denotes the
T8050 31532-31537 NN denotes Sam68
T8051 31538-31548 JJ denotes regulatory
T8049 31549-31556 NN denotes protein
T8052 31556-31558 , denotes ,
T8053 31558-31561 NN denotes Src
T8054 31561-31563 , denotes ,
T8055 31563-31565 IN denotes in
T8056 31566-31579 NN denotes osteopetrosis
T8046 31580-31583 VBD denotes led
T8057 31584-31586 PRP denotes us
T8058 31587-31589 TO denotes to
T8059 31590-31601 VB denotes investigate
T8060 31602-31605 DT denotes the
T8061 31606-31616 NN denotes morphology
T8062 31617-31620 CC denotes and
T8063 31621-31629 NN denotes activity
T8064 31630-31632 IN denotes of
T8065 31633-31638 NN denotes Sam68
T8067 31638-31639 SYM denotes −
T8068 31639-31640 HYPH denotes /
T8069 31640-31641 SYM denotes −
T8066 31642-31653 NNS denotes osteoclasts
T8070 31654-31656 FW denotes ex
T8071 31657-31661 FW denotes vivo
T8072 31661-31662 . denotes .
T8073 31662-31880 sentence denotes The Src tyrosine kinase was shown to play a role in bone remodeling when Src−/− mice died at 6 months of age with an osteopetrotic phenotype [48] and the defect was attributed to defective osteoclast function [55−59].
T8074 31663-31666 DT denotes The
T8076 31667-31670 NN denotes Src
T8077 31671-31679 NN denotes tyrosine
T8075 31680-31686 NN denotes kinase
T8079 31687-31690 VBD denotes was
T8078 31691-31696 VBN denotes shown
T8080 31697-31699 TO denotes to
T8081 31700-31704 VB denotes play
T8082 31705-31706 DT denotes a
T8083 31707-31711 NN denotes role
T8084 31712-31714 IN denotes in
T8085 31715-31719 NN denotes bone
T8086 31720-31730 NN denotes remodeling
T8087 31731-31735 WRB denotes when
T8089 31736-31739 NN denotes Src
T8091 31739-31740 SYM denotes −
T8092 31740-31741 HYPH denotes /
T8093 31741-31742 SYM denotes −
T8090 31743-31747 NNS denotes mice
T8088 31748-31752 VBD denotes died
T8094 31753-31755 IN denotes at
T8095 31756-31757 CD denotes 6
T8096 31758-31764 NNS denotes months
T8097 31765-31767 IN denotes of
T8098 31768-31771 NN denotes age
T8099 31772-31776 IN denotes with
T8100 31777-31779 DT denotes an
T8102 31780-31793 JJ denotes osteopetrotic
T8101 31794-31803 NN denotes phenotype
T8103 31804-31805 -LRB- denotes [
T8104 31805-31807 CD denotes 48
T8105 31807-31808 -RRB- denotes ]
T8106 31809-31812 CC denotes and
T8107 31813-31816 DT denotes the
T8108 31817-31823 NN denotes defect
T8110 31824-31827 VBD denotes was
T8109 31828-31838 VBN denotes attributed
T8111 31839-31841 IN denotes to
T8112 31842-31851 JJ denotes defective
T8114 31852-31862 NN denotes osteoclast
T8113 31863-31871 NN denotes function
T8115 31872-31873 -LRB- denotes [
T8116 31873-31875 CD denotes 55
T8117 31875-31876 SYM denotes −
T8118 31876-31878 CD denotes 59
T8119 31878-31879 -RRB- denotes ]
T8120 31879-31880 . denotes .
T8121 31880-32027 sentence denotes We therefore cultured mature osteoclasts harvested from Sam68+/+ and Sam68−/− mice ex vivo on dentin slices to quantify their resorptive capacity.
T8122 31881-31883 PRP denotes We
T8124 31884-31893 RB denotes therefore
T8123 31894-31902 VBD denotes cultured
T8125 31903-31909 JJ denotes mature
T8126 31910-31921 NNS denotes osteoclasts
T8127 31922-31931 VBN denotes harvested
T8128 31932-31936 IN denotes from
T8129 31937-31942 NN denotes Sam68
T8131 31942-31943 SYM denotes +
T8132 31943-31944 HYPH denotes /
T8133 31944-31945 SYM denotes +
T8134 31946-31949 CC denotes and
T8135 31950-31955 NN denotes Sam68
T8136 31955-31956 SYM denotes −
T8137 31956-31957 HYPH denotes /
T8138 31957-31958 SYM denotes −
T8130 31959-31963 NNS denotes mice
T8139 31964-31966 FW denotes ex
T8140 31967-31971 FW denotes vivo
T8141 31972-31974 IN denotes on
T8142 31975-31981 NN denotes dentin
T8143 31982-31988 NNS denotes slices
T8144 31989-31991 TO denotes to
T8145 31992-32000 VB denotes quantify
T8146 32001-32006 PRP$ denotes their
T8148 32007-32017 JJ denotes resorptive
T8147 32018-32026 NN denotes capacity
T8149 32026-32027 . denotes .
T8150 32027-32243 sentence denotes The fact that Sam68−/− osteoclasts looked and acted like Sam68+/+ osteoclasts ex vivo and in vivo made it unlikely that this was the primary source of the difference in bone metabolism in 12-month-old Sam68−/− mice.
T8151 32028-32031 DT denotes The
T8152 32032-32036 NN denotes fact
T8154 32037-32041 IN denotes that
T8156 32042-32047 NN denotes Sam68
T8158 32047-32048 SYM denotes −
T8159 32048-32049 HYPH denotes /
T8160 32049-32050 SYM denotes −
T8157 32051-32062 NNS denotes osteoclasts
T8155 32063-32069 VBD denotes looked
T8161 32070-32073 CC denotes and
T8162 32074-32079 VBD denotes acted
T8163 32080-32084 IN denotes like
T8164 32085-32090 NN denotes Sam68
T8166 32090-32091 SYM denotes +
T8167 32091-32092 HYPH denotes /
T8168 32092-32093 SYM denotes +
T8165 32094-32105 NNS denotes osteoclasts
T8169 32106-32108 FW denotes ex
T8170 32109-32113 FW denotes vivo
T8171 32114-32117 CC denotes and
T8172 32118-32120 FW denotes in
T8173 32121-32125 FW denotes vivo
T8153 32126-32130 VBD denotes made
T8174 32131-32133 PRP denotes it
T8175 32134-32142 JJ denotes unlikely
T8176 32143-32147 IN denotes that
T8178 32148-32152 DT denotes this
T8177 32153-32156 VBD denotes was
T8179 32157-32160 DT denotes the
T8181 32161-32168 JJ denotes primary
T8180 32169-32175 NN denotes source
T8182 32176-32178 IN denotes of
T8183 32179-32182 DT denotes the
T8184 32183-32193 NN denotes difference
T8185 32194-32196 IN denotes in
T8186 32197-32201 NN denotes bone
T8187 32202-32212 NN denotes metabolism
T8188 32213-32215 IN denotes in
T8189 32216-32218 CD denotes 12
T8191 32218-32219 HYPH denotes -
T8190 32219-32224 NN denotes month
T8193 32224-32225 HYPH denotes -
T8192 32225-32228 JJ denotes old
T8195 32229-32234 NN denotes Sam68
T8196 32234-32235 SYM denotes −
T8197 32235-32236 HYPH denotes /
T8198 32236-32237 SYM denotes −
T8194 32238-32242 NNS denotes mice
T8199 32242-32243 . denotes .
T8200 32243-32407 sentence denotes The fact that the CTX levels were lower in young and old Sam68−/− mice suggested that there is reduced bone resorption compared with wild-type littermate controls.
T8201 32244-32247 DT denotes The
T8202 32248-32252 NN denotes fact
T8204 32253-32257 IN denotes that
T8206 32258-32261 DT denotes the
T8208 32262-32265 NN denotes CTX
T8207 32266-32272 NNS denotes levels
T8205 32273-32277 VBD denotes were
T8209 32278-32283 JJR denotes lower
T8210 32284-32286 IN denotes in
T8211 32287-32292 JJ denotes young
T8213 32293-32296 CC denotes and
T8214 32297-32300 JJ denotes old
T8215 32301-32306 NN denotes Sam68
T8216 32306-32307 SYM denotes −
T8217 32307-32308 HYPH denotes /
T8218 32308-32309 SYM denotes −
T8212 32310-32314 NNS denotes mice
T8203 32315-32324 VBD denotes suggested
T8219 32325-32329 IN denotes that
T8221 32330-32335 EX denotes there
T8220 32336-32338 VBZ denotes is
T8222 32339-32346 VBN denotes reduced
T8224 32347-32351 NN denotes bone
T8223 32352-32362 NN denotes resorption
T8225 32363-32371 VBN denotes compared
T8226 32372-32376 IN denotes with
T8227 32377-32381 JJ denotes wild
T8229 32381-32382 HYPH denotes -
T8228 32382-32386 NN denotes type
T8231 32387-32397 NN denotes littermate
T8230 32398-32406 NNS denotes controls
T8232 32406-32407 . denotes .
T8233 32407-32527 sentence denotes However, this reduction in bone resorption occurred with normal osteoclast activity, as assessed by in vitro culturing.
T8234 32408-32415 RB denotes However
T8236 32415-32417 , denotes ,
T8237 32417-32421 DT denotes this
T8238 32422-32431 NN denotes reduction
T8239 32432-32434 IN denotes in
T8240 32435-32439 NN denotes bone
T8241 32440-32450 NN denotes resorption
T8235 32451-32459 VBD denotes occurred
T8242 32460-32464 IN denotes with
T8243 32465-32471 JJ denotes normal
T8245 32472-32482 NN denotes osteoclast
T8244 32483-32491 NN denotes activity
T8246 32491-32493 , denotes ,
T8247 32493-32495 IN denotes as
T8248 32496-32504 VBN denotes assessed
T8249 32505-32507 IN denotes by
T8250 32508-32510 FW denotes in
T8251 32511-32516 FW denotes vitro
T8252 32517-32526 NN denotes culturing
T8253 32526-32527 . denotes .
T8254 32527-32620 sentence denotes These observations are consistent with the Sam68−/− mice having a youth-like bone phenotype.
T8255 32528-32533 DT denotes These
T8256 32534-32546 NNS denotes observations
T8257 32547-32550 VBP denotes are
T8258 32551-32561 JJ denotes consistent
T8259 32562-32566 IN denotes with
T8260 32567-32570 DT denotes the
T8262 32571-32576 NN denotes Sam68
T8263 32576-32577 SYM denotes −
T8264 32577-32578 HYPH denotes /
T8265 32578-32579 SYM denotes −
T8261 32580-32584 NNS denotes mice
T8266 32585-32591 VBG denotes having
T8267 32592-32593 DT denotes a
T8269 32594-32599 NN denotes youth
T8271 32599-32600 HYPH denotes -
T8270 32600-32604 JJ denotes like
T8272 32605-32609 NN denotes bone
T8268 32610-32619 NN denotes phenotype
T8273 32619-32620 . denotes .
T8274 32620-32825 sentence denotes However, it is still possible, that a mild impairment in Sam68−/− mice osteoclast function may manifest itself later in life in overall accumulation of bone and this will require further detailed studies.
T8275 32621-32628 RB denotes However
T8277 32628-32630 , denotes ,
T8278 32630-32632 PRP denotes it
T8276 32633-32635 VBZ denotes is
T8279 32636-32641 RB denotes still
T8280 32642-32650 JJ denotes possible
T8281 32650-32652 , denotes ,
T8282 32652-32656 IN denotes that
T8284 32657-32658 DT denotes a
T8286 32659-32663 JJ denotes mild
T8285 32664-32674 NN denotes impairment
T8287 32675-32677 IN denotes in
T8288 32678-32683 NN denotes Sam68
T8290 32683-32684 SYM denotes −
T8291 32684-32685 HYPH denotes /
T8292 32685-32686 SYM denotes −
T8289 32687-32691 NNS denotes mice
T8294 32692-32702 NN denotes osteoclast
T8293 32703-32711 NN denotes function
T8295 32712-32715 MD denotes may
T8283 32716-32724 VB denotes manifest
T8296 32725-32731 PRP denotes itself
T8297 32732-32737 RB denotes later
T8298 32738-32740 IN denotes in
T8299 32741-32745 NN denotes life
T8300 32746-32748 IN denotes in
T8301 32749-32756 JJ denotes overall
T8302 32757-32769 NN denotes accumulation
T8303 32770-32772 IN denotes of
T8304 32773-32777 NN denotes bone
T8305 32778-32781 CC denotes and
T8306 32782-32786 DT denotes this
T8308 32787-32791 MD denotes will
T8307 32792-32799 VB denotes require
T8309 32800-32807 JJ denotes further
T8311 32808-32816 JJ denotes detailed
T8310 32817-32824 NNS denotes studies
T8312 32824-32825 . denotes .
T8313 32825-33048 sentence denotes Ex vivo differentiation of primary bone marrow stromal cells, harvested from Sam68+/+ and Sam68−/− mice, down the osteoblast lineage revealed an osteogenic advantage in the cultures of cells derived from the Sam68−/− mice.
T8314 32826-32828 FW denotes Ex
T8315 32829-32833 FW denotes vivo
T8316 32834-32849 NN denotes differentiation
T8318 32850-32852 IN denotes of
T8319 32853-32860 JJ denotes primary
T8321 32861-32865 NN denotes bone
T8322 32866-32872 NN denotes marrow
T8323 32873-32880 JJ denotes stromal
T8320 32881-32886 NNS denotes cells
T8324 32886-32888 , denotes ,
T8325 32888-32897 VBN denotes harvested
T8326 32898-32902 IN denotes from
T8327 32903-32908 NN denotes Sam68
T8329 32908-32909 SYM denotes +
T8330 32909-32910 HYPH denotes /
T8331 32910-32911 SYM denotes +
T8332 32912-32915 CC denotes and
T8333 32916-32921 NN denotes Sam68
T8334 32921-32922 SYM denotes −
T8335 32922-32923 HYPH denotes /
T8336 32923-32924 SYM denotes −
T8328 32925-32929 NNS denotes mice
T8337 32929-32931 , denotes ,
T8338 32931-32935 IN denotes down
T8339 32936-32939 DT denotes the
T8341 32940-32950 NN denotes osteoblast
T8340 32951-32958 NN denotes lineage
T8317 32959-32967 VBD denotes revealed
T8342 32968-32970 DT denotes an
T8344 32971-32981 JJ denotes osteogenic
T8343 32982-32991 NN denotes advantage
T8345 32992-32994 IN denotes in
T8346 32995-32998 DT denotes the
T8347 32999-33007 NNS denotes cultures
T8348 33008-33010 IN denotes of
T8349 33011-33016 NNS denotes cells
T8350 33017-33024 VBN denotes derived
T8351 33025-33029 IN denotes from
T8352 33030-33033 DT denotes the
T8354 33034-33039 NN denotes Sam68
T8355 33039-33040 SYM denotes −
T8356 33040-33041 HYPH denotes /
T8357 33041-33042 SYM denotes −
T8353 33043-33047 NNS denotes mice
T8358 33047-33048 . denotes .
T8359 33048-33183 sentence denotes It will be important to demonstrate that a similar effect is observed using primary bone marrow stromal cells from aged Sam68−/− mice.
T8360 33049-33051 PRP denotes It
T8362 33052-33056 MD denotes will
T8361 33057-33059 VB denotes be
T8363 33060-33069 JJ denotes important
T8364 33070-33072 TO denotes to
T8365 33073-33084 VB denotes demonstrate
T8366 33085-33089 IN denotes that
T8368 33090-33091 DT denotes a
T8370 33092-33099 JJ denotes similar
T8369 33100-33106 NN denotes effect
T8371 33107-33109 VBZ denotes is
T8367 33110-33118 VBN denotes observed
T8372 33119-33124 VBG denotes using
T8373 33125-33132 JJ denotes primary
T8375 33133-33137 NN denotes bone
T8376 33138-33144 NN denotes marrow
T8377 33145-33152 JJ denotes stromal
T8374 33153-33158 NNS denotes cells
T8378 33159-33163 IN denotes from
T8379 33164-33168 JJ denotes aged
T8381 33169-33174 NN denotes Sam68
T8382 33174-33175 SYM denotes −
T8383 33175-33176 HYPH denotes /
T8384 33176-33177 SYM denotes −
T8380 33178-33182 NNS denotes mice
T8385 33182-33183 . denotes .
T8386 33183-33357 sentence denotes Similar findings were observed when embryonic mesenchymal multipotential progenitor cells C3H10T1/2 treated with Sam68 shRNA, were differentiated into osteoblasts with BMP2.
T8387 33184-33191 JJ denotes Similar
T8388 33192-33200 NNS denotes findings
T8390 33201-33205 VBD denotes were
T8389 33206-33214 VBN denotes observed
T8391 33215-33219 WRB denotes when
T8393 33220-33229 JJ denotes embryonic
T8395 33230-33241 JJ denotes mesenchymal
T8396 33242-33256 JJ denotes multipotential
T8397 33257-33267 NN denotes progenitor
T8394 33268-33273 NNS denotes cells
T8398 33274-33281 NN denotes C3H10T1
T8399 33281-33282 HYPH denotes /
T8400 33282-33283 CD denotes 2
T8401 33284-33291 VBN denotes treated
T8402 33292-33296 IN denotes with
T8403 33297-33302 NN denotes Sam68
T8404 33303-33308 NN denotes shRNA
T8405 33308-33310 , denotes ,
T8406 33310-33314 VBD denotes were
T8392 33315-33329 VBN denotes differentiated
T8407 33330-33334 IN denotes into
T8408 33335-33346 NNS denotes osteoblasts
T8409 33347-33351 IN denotes with
T8410 33352-33356 NN denotes BMP2
T8411 33356-33357 . denotes .
T8412 33357-33587 sentence denotes Our findings that aged Sam68−/− mice do not develop fatty bone marrow and that MEFs derived from Sam68 −/− mice have impaired adipocyte differentiation, indicate that Sam68 regulates both adipocyte and osteoblast differentiation.
T8413 33358-33361 PRP$ denotes Our
T8414 33362-33370 NNS denotes findings
T8416 33371-33375 IN denotes that
T8418 33376-33380 JJ denotes aged
T8420 33381-33386 NN denotes Sam68
T8421 33386-33387 SYM denotes −
T8422 33387-33388 HYPH denotes /
T8423 33388-33389 SYM denotes −
T8419 33390-33394 NNS denotes mice
T8424 33395-33397 VBP denotes do
T8425 33398-33401 RB denotes not
T8417 33402-33409 VB denotes develop
T8426 33410-33415 JJ denotes fatty
T8428 33416-33420 NN denotes bone
T8427 33421-33427 NN denotes marrow
T8429 33428-33431 CC denotes and
T8430 33432-33436 IN denotes that
T8432 33437-33441 NNS denotes MEFs
T8433 33442-33449 VBN denotes derived
T8434 33450-33454 IN denotes from
T8435 33455-33460 NN denotes Sam68
T8437 33461-33462 SYM denotes −
T8438 33462-33463 HYPH denotes /
T8439 33463-33464 SYM denotes −
T8436 33465-33469 NNS denotes mice
T8431 33470-33474 VBP denotes have
T8440 33475-33483 VBN denotes impaired
T8442 33484-33493 NN denotes adipocyte
T8441 33494-33509 NN denotes differentiation
T8443 33509-33511 , denotes ,
T8415 33511-33519 VBP denotes indicate
T8444 33520-33524 IN denotes that
T8446 33525-33530 NN denotes Sam68
T8445 33531-33540 VBZ denotes regulates
T8447 33541-33545 CC denotes both
T8448 33546-33555 NN denotes adipocyte
T8450 33556-33559 CC denotes and
T8451 33560-33570 NN denotes osteoblast
T8449 33571-33586 NN denotes differentiation
T8452 33586-33587 . denotes .
T8453 33587-33797 sentence denotes These findings identify Sam68 as the first RNA binding protein to regulate mesenchymal cell differentiation and the challenge will be to identify the specific RNA targets that it regulates during this process.
T8454 33588-33593 DT denotes These
T8455 33594-33602 NNS denotes findings
T8456 33603-33611 VBP denotes identify
T8457 33612-33617 NN denotes Sam68
T8458 33618-33620 IN denotes as
T8459 33621-33624 DT denotes the
T8461 33625-33630 JJ denotes first
T8462 33631-33634 NN denotes RNA
T8463 33635-33642 VBG denotes binding
T8460 33643-33650 NN denotes protein
T8464 33651-33653 TO denotes to
T8465 33654-33662 VB denotes regulate
T8466 33663-33674 JJ denotes mesenchymal
T8468 33675-33679 NN denotes cell
T8467 33680-33695 NN denotes differentiation
T8469 33696-33699 CC denotes and
T8470 33700-33703 DT denotes the
T8471 33704-33713 NN denotes challenge
T8473 33714-33718 MD denotes will
T8472 33719-33721 VB denotes be
T8474 33722-33724 TO denotes to
T8475 33725-33733 VB denotes identify
T8476 33734-33737 DT denotes the
T8478 33738-33746 JJ denotes specific
T8479 33747-33750 NN denotes RNA
T8477 33751-33758 NNS denotes targets
T8480 33759-33763 WDT denotes that
T8482 33764-33766 PRP denotes it
T8481 33767-33776 VBZ denotes regulates
T8483 33777-33783 IN denotes during
T8484 33784-33788 DT denotes this
T8485 33789-33796 NN denotes process
T8486 33796-33797 . denotes .
T8487 33797-33983 sentence denotes The fact that osteoblast function is altered in Src−/− mice [60,61] raises the possibility that preservation of bone mass in the Sam68−/− mice could be linked with and regulated by Src.
T8488 33798-33801 DT denotes The
T8489 33802-33806 NN denotes fact
T8491 33807-33811 IN denotes that
T8493 33812-33822 NN denotes osteoblast
T8494 33823-33831 NN denotes function
T8495 33832-33834 VBZ denotes is
T8492 33835-33842 VBN denotes altered
T8496 33843-33845 IN denotes in
T8497 33846-33849 NN denotes Src
T8499 33849-33850 SYM denotes −
T8500 33850-33851 HYPH denotes /
T8501 33851-33852 SYM denotes −
T8498 33853-33857 NNS denotes mice
T8502 33858-33859 -LRB- denotes [
T8504 33859-33861 CD denotes 60
T8505 33861-33862 , denotes ,
T8503 33862-33864 CD denotes 61
T8506 33864-33865 -RRB- denotes ]
T8490 33866-33872 VBZ denotes raises
T8507 33873-33876 DT denotes the
T8508 33877-33888 NN denotes possibility
T8509 33889-33893 IN denotes that
T8511 33894-33906 NN denotes preservation
T8512 33907-33909 IN denotes of
T8513 33910-33914 NN denotes bone
T8514 33915-33919 NN denotes mass
T8515 33920-33922 IN denotes in
T8516 33923-33926 DT denotes the
T8518 33927-33932 NN denotes Sam68
T8519 33932-33933 SYM denotes −
T8520 33933-33934 HYPH denotes /
T8521 33934-33935 SYM denotes −
T8517 33936-33940 NNS denotes mice
T8522 33941-33946 MD denotes could
T8523 33947-33949 VB denotes be
T8510 33950-33956 VBN denotes linked
T8524 33957-33961 IN denotes with
T8525 33962-33965 CC denotes and
T8526 33966-33975 VBN denotes regulated
T8527 33976-33978 IN denotes by
T8528 33979-33982 NN denotes Src
T8529 33982-33983 . denotes .
T8530 33983-34257 sentence denotes The pathway by which leptin regulates bone resorption was identified to involve the sympathetic nervous system relaying to the osteoblasts via the β-adrenergic pathway leading to the release of growth factors including RANKL that causes the osteoclasts to thrive [8,18–20].
T8531 33984-33987 DT denotes The
T8532 33988-33995 NN denotes pathway
T8534 33996-33998 IN denotes by
T8536 33999-34004 WDT denotes which
T8537 34005-34011 NN denotes leptin
T8535 34012-34021 VBZ denotes regulates
T8538 34022-34026 NN denotes bone
T8539 34027-34037 NN denotes resorption
T8540 34038-34041 VBD denotes was
T8533 34042-34052 VBN denotes identified
T8541 34053-34055 TO denotes to
T8542 34056-34063 VB denotes involve
T8543 34064-34067 DT denotes the
T8545 34068-34079 JJ denotes sympathetic
T8546 34080-34087 JJ denotes nervous
T8544 34088-34094 NN denotes system
T8547 34095-34103 VBG denotes relaying
T8548 34104-34106 IN denotes to
T8549 34107-34110 DT denotes the
T8550 34111-34122 NNS denotes osteoblasts
T8551 34123-34126 IN denotes via
T8552 34127-34130 DT denotes the
T8554 34131-34132 NN denotes β
T8556 34132-34133 HYPH denotes -
T8555 34133-34143 JJ denotes adrenergic
T8553 34144-34151 NN denotes pathway
T8557 34152-34159 VBG denotes leading
T8558 34160-34162 IN denotes to
T8559 34163-34166 DT denotes the
T8560 34167-34174 NN denotes release
T8561 34175-34177 IN denotes of
T8562 34178-34184 NN denotes growth
T8563 34185-34192 NNS denotes factors
T8564 34193-34202 VBG denotes including
T8565 34203-34208 NN denotes RANKL
T8566 34209-34213 WDT denotes that
T8567 34214-34220 VBZ denotes causes
T8568 34221-34224 DT denotes the
T8569 34225-34236 NNS denotes osteoclasts
T8571 34237-34239 TO denotes to
T8570 34240-34246 VB denotes thrive
T8572 34247-34248 -LRB- denotes [
T8573 34248-34249 CD denotes 8
T8574 34249-34250 , denotes ,
T8575 34250-34252 CD denotes 18
T8576 34252-34253 SYM denotes –
T8577 34253-34255 CD denotes 20
T8578 34255-34256 -RRB- denotes ]
T8579 34256-34257 . denotes .
T8580 34257-34401 sentence denotes The lowering of leptin levels in aged Sam68−/− mice is consistent with these mice having a high bone mass compared with their aged littermates.
T8581 34258-34261 DT denotes The
T8582 34262-34270 NN denotes lowering
T8584 34271-34273 IN denotes of
T8585 34274-34280 NN denotes leptin
T8586 34281-34287 NNS denotes levels
T8587 34288-34290 IN denotes in
T8588 34291-34295 JJ denotes aged
T8590 34296-34301 NN denotes Sam68
T8591 34301-34302 SYM denotes −
T8592 34302-34303 HYPH denotes /
T8593 34303-34304 SYM denotes −
T8589 34305-34309 NNS denotes mice
T8583 34310-34312 VBZ denotes is
T8594 34313-34323 JJ denotes consistent
T8595 34324-34328 IN denotes with
T8596 34329-34334 DT denotes these
T8597 34335-34339 NNS denotes mice
T8598 34340-34346 VBG denotes having
T8599 34347-34348 DT denotes a
T8601 34349-34353 JJ denotes high
T8602 34354-34358 NN denotes bone
T8600 34359-34363 NN denotes mass
T8603 34364-34372 VBN denotes compared
T8604 34373-34377 IN denotes with
T8605 34378-34383 PRP$ denotes their
T8607 34384-34388 JJ denotes aged
T8606 34389-34400 NNS denotes littermates
T8608 34400-34401 . denotes .
T8609 34401-34591 sentence denotes These data would suggest that the leptin-sympathetic pathway is unaltered in Sam68−/− mice and that the lowering of leptin may explain the lower levels of CTX in the serum of Sam68−/− mice.
T8610 34402-34407 DT denotes These
T8611 34408-34412 NNS denotes data
T8613 34413-34418 MD denotes would
T8612 34419-34426 VB denotes suggest
T8614 34427-34431 IN denotes that
T8616 34432-34435 DT denotes the
T8618 34436-34442 NN denotes leptin
T8620 34442-34443 HYPH denotes -
T8619 34443-34454 JJ denotes sympathetic
T8617 34455-34462 NN denotes pathway
T8615 34463-34465 VBZ denotes is
T8621 34466-34475 JJ denotes unaltered
T8622 34476-34478 IN denotes in
T8623 34479-34484 NN denotes Sam68
T8625 34484-34485 SYM denotes −
T8626 34485-34486 HYPH denotes /
T8627 34486-34487 SYM denotes −
T8624 34488-34492 NNS denotes mice
T8628 34493-34496 CC denotes and
T8629 34497-34501 IN denotes that
T8631 34502-34505 DT denotes the
T8632 34506-34514 NN denotes lowering
T8633 34515-34517 IN denotes of
T8634 34518-34524 NN denotes leptin
T8635 34525-34528 MD denotes may
T8630 34529-34536 VB denotes explain
T8636 34537-34540 DT denotes the
T8638 34541-34546 JJR denotes lower
T8637 34547-34553 NNS denotes levels
T8639 34554-34556 IN denotes of
T8640 34557-34560 NN denotes CTX
T8641 34561-34563 IN denotes in
T8642 34564-34567 DT denotes the
T8643 34568-34573 NN denotes serum
T8644 34574-34576 IN denotes of
T8645 34577-34582 NN denotes Sam68
T8647 34582-34583 SYM denotes −
T8648 34583-34584 HYPH denotes /
T8649 34584-34585 SYM denotes −
T8646 34586-34590 NNS denotes mice
T8650 34590-34591 . denotes .
T8651 34591-34895 sentence denotes These findings suggest that Sam68 may be regulating bone metabolism at two different levels: (1) the absence of Sam68 results in lower leptin levels that may reduce bone resorption via the sympathetic nervous system and (2) the absence of Sam68 favors osteoblast, rather than adipocyte, differentiation.
T8652 34592-34597 DT denotes These
T8653 34598-34606 NNS denotes findings
T8654 34607-34614 VBP denotes suggest
T8656 34615-34619 IN denotes that
T8658 34620-34625 NN denotes Sam68
T8659 34626-34629 MD denotes may
T8660 34630-34632 VB denotes be
T8657 34633-34643 VBG denotes regulating
T8661 34644-34648 NN denotes bone
T8662 34649-34659 NN denotes metabolism
T8663 34660-34662 IN denotes at
T8664 34663-34666 CD denotes two
T8666 34667-34676 JJ denotes different
T8665 34677-34683 NNS denotes levels
T8667 34683-34685 : denotes :
T8668 34685-34686 -LRB- denotes (
T8669 34686-34687 LS denotes 1
T8670 34687-34688 -RRB- denotes )
T8671 34689-34692 DT denotes the
T8672 34693-34700 NN denotes absence
T8673 34701-34703 IN denotes of
T8674 34704-34709 NN denotes Sam68
T8655 34710-34717 VBZ denotes results
T8675 34718-34720 IN denotes in
T8676 34721-34726 JJR denotes lower
T8678 34727-34733 NN denotes leptin
T8677 34734-34740 NNS denotes levels
T8679 34741-34745 WDT denotes that
T8681 34746-34749 MD denotes may
T8680 34750-34756 VB denotes reduce
T8682 34757-34761 NN denotes bone
T8683 34762-34772 NN denotes resorption
T8684 34773-34776 IN denotes via
T8685 34777-34780 DT denotes the
T8687 34781-34792 JJ denotes sympathetic
T8688 34793-34800 JJ denotes nervous
T8686 34801-34807 NN denotes system
T8689 34808-34811 CC denotes and
T8690 34812-34813 -LRB- denotes (
T8691 34813-34814 LS denotes 2
T8693 34814-34815 -RRB- denotes )
T8694 34816-34819 DT denotes the
T8695 34820-34827 NN denotes absence
T8696 34828-34830 IN denotes of
T8697 34831-34836 NN denotes Sam68
T8692 34837-34843 VBZ denotes favors
T8698 34844-34854 NN denotes osteoblast
T8700 34854-34856 , denotes ,
T8701 34856-34862 RB denotes rather
T8702 34863-34867 IN denotes than
T8703 34868-34877 NN denotes adipocyte
T8704 34877-34879 , denotes ,
T8699 34879-34894 NN denotes differentiation
T8705 34894-34895 . denotes .
T8706 34895-35029 sentence denotes In conclusion, our data define a physiologic role for Sam68 in bone metabolism and bone marrow mesenchymal stem cell differentiation.
T8707 34896-34898 IN denotes In
T8709 34899-34909 NN denotes conclusion
T8710 34909-34911 , denotes ,
T8711 34911-34914 PRP$ denotes our
T8712 34915-34919 NNS denotes data
T8708 34920-34926 VBP denotes define
T8713 34927-34928 DT denotes a
T8715 34929-34940 JJ denotes physiologic
T8714 34941-34945 NN denotes role
T8716 34946-34949 IN denotes for
T8717 34950-34955 NN denotes Sam68
T8718 34956-34958 IN denotes in
T8719 34959-34963 NN denotes bone
T8720 34964-34974 NN denotes metabolism
T8721 34975-34978 CC denotes and
T8722 34979-34983 NN denotes bone
T8723 34984-34990 NN denotes marrow
T8725 34991-35002 JJ denotes mesenchymal
T8727 35003-35007 NN denotes stem
T8726 35008-35012 NN denotes cell
T8724 35013-35028 NN denotes differentiation
T8728 35028-35029 . denotes .
T8729 35029-35142 sentence denotes The bone phenotype observed in Sam68−/− mice imply that inhibitors of Sam68 could prevent age-related bone loss.
T8730 35030-35033 DT denotes The
T8732 35034-35038 NN denotes bone
T8731 35039-35048 NN denotes phenotype
T8734 35049-35057 VBN denotes observed
T8735 35058-35060 IN denotes in
T8736 35061-35066 NN denotes Sam68
T8738 35066-35067 SYM denotes −
T8739 35067-35068 HYPH denotes /
T8740 35068-35069 SYM denotes −
T8737 35070-35074 NNS denotes mice
T8733 35075-35080 VBP denotes imply
T8741 35081-35085 IN denotes that
T8743 35086-35096 NNS denotes inhibitors
T8744 35097-35099 IN denotes of
T8745 35100-35105 NN denotes Sam68
T8746 35106-35111 MD denotes could
T8742 35112-35119 VB denotes prevent
T8747 35120-35123 NN denotes age
T8749 35123-35124 HYPH denotes -
T8748 35124-35131 VBN denotes related
T8751 35132-35136 NN denotes bone
T8750 35137-35141 NN denotes loss
T8752 35141-35142 . denotes .
T8753 35142-35328 sentence denotes Furthermore, the results also suggest that Sam68 expression levels, hypomorphism, and mutations in humans may influence susceptibility to marrow adipocyte accumulation and osteoporosis.
T8754 35143-35154 RB denotes Furthermore
T8756 35154-35156 , denotes ,
T8757 35156-35159 DT denotes the
T8758 35160-35167 NNS denotes results
T8759 35168-35172 RB denotes also
T8755 35173-35180 VBP denotes suggest
T8760 35181-35185 IN denotes that
T8762 35186-35191 NN denotes Sam68
T8764 35192-35202 NN denotes expression
T8763 35203-35209 NNS denotes levels
T8765 35209-35211 , denotes ,
T8766 35211-35223 NN denotes hypomorphism
T8767 35223-35225 , denotes ,
T8768 35225-35228 CC denotes and
T8769 35229-35238 NNS denotes mutations
T8770 35239-35241 IN denotes in
T8771 35242-35248 NNS denotes humans
T8772 35249-35252 MD denotes may
T8761 35253-35262 VB denotes influence
T8773 35263-35277 NN denotes susceptibility
T8774 35278-35280 IN denotes to
T8775 35281-35287 NN denotes marrow
T8776 35288-35297 NN denotes adipocyte
T8777 35298-35310 NN denotes accumulation
T8778 35311-35314 CC denotes and
T8779 35315-35327 NN denotes osteoporosis
T8780 35327-35328 . denotes .
T8781 35328-35400 sentence denotes Our findings also identify a new animal model to study aging bone loss.
T8782 35329-35332 PRP$ denotes Our
T8783 35333-35341 NNS denotes findings
T8785 35342-35346 RB denotes also
T8784 35347-35355 VBP denotes identify
T8786 35356-35357 DT denotes a
T8788 35358-35361 JJ denotes new
T8789 35362-35368 NN denotes animal
T8787 35369-35374 NN denotes model
T8790 35375-35377 TO denotes to
T8791 35378-35383 VB denotes study
T8792 35384-35389 VBG denotes aging
T8794 35390-35394 NN denotes bone
T8793 35395-35399 NN denotes loss
T8795 35399-35400 . denotes .
T8863 35425-35435 JJ denotes Histologic
T8865 35435-35437 , denotes ,
T8866 35437-35456 JJ denotes immunohistochemical
T8867 35456-35458 , denotes ,
T8868 35458-35461 CC denotes and
T8869 35462-35479 JJ denotes histomorphometric
T8864 35480-35488 NNS denotes analyses
T8870 35488-35489 . denotes .
T8871 35489-35562 sentence denotes All analyses were performed essentially as described previously [62,63].
T8872 35490-35493 DT denotes All
T8873 35494-35502 NNS denotes analyses
T8875 35503-35507 VBD denotes were
T8874 35508-35517 VBN denotes performed
T8876 35518-35529 RB denotes essentially
T8878 35530-35532 IN denotes as
T8877 35533-35542 VBN denotes described
T8879 35543-35553 RB denotes previously
T8880 35554-35555 -LRB- denotes [
T8882 35555-35557 CD denotes 62
T8883 35557-35558 , denotes ,
T8881 35558-35560 CD denotes 63
T8884 35560-35561 -RRB- denotes ]
T8885 35561-35562 . denotes .
T8886 35562-35757 sentence denotes Briefly, embryonic mice were removed from timed pregnant dams at E14.5 and E16.5 and fixed intact for 36 h in 4% paraformaldehyde, rinsed thoroughly in PBS, and processed for paraffin embedding.
T8887 35563-35570 RB denotes Briefly
T8889 35570-35572 , denotes ,
T8890 35572-35581 JJ denotes embryonic
T8891 35582-35586 NNS denotes mice
T8892 35587-35591 VBD denotes were
T8888 35592-35599 VBN denotes removed
T8893 35600-35604 IN denotes from
T8894 35605-35610 VBN denotes timed
T8896 35611-35619 JJ denotes pregnant
T8895 35620-35624 NNS denotes dams
T8897 35625-35627 IN denotes at
T8898 35628-35633 NN denotes E14.5
T8899 35634-35637 CC denotes and
T8900 35638-35643 NN denotes E16.5
T8901 35644-35647 CC denotes and
T8902 35648-35653 VBN denotes fixed
T8903 35654-35660 JJ denotes intact
T8904 35661-35664 IN denotes for
T8905 35665-35667 CD denotes 36
T8906 35668-35669 NN denotes h
T8907 35670-35672 IN denotes in
T8908 35673-35674 CD denotes 4
T8909 35674-35675 NN denotes %
T8910 35676-35692 NN denotes paraformaldehyde
T8911 35692-35694 , denotes ,
T8912 35694-35700 VBN denotes rinsed
T8913 35701-35711 RB denotes thoroughly
T8914 35712-35714 IN denotes in
T8915 35715-35718 NN denotes PBS
T8916 35718-35720 , denotes ,
T8917 35720-35723 CC denotes and
T8918 35724-35733 VBN denotes processed
T8919 35734-35737 IN denotes for
T8920 35738-35746 NN denotes paraffin
T8921 35747-35756 NN denotes embedding
T8922 35756-35757 . denotes .
T8923 35757-36012 sentence denotes Serial 4-μm sections were cut on a modified Leica RM 2155 rotary microtome (Leica Microsystems, Richmond Hill, Ontario, Canada), stained with a 1:600 dilution of the AD1 anti-Sam68 antibody [46] and counterstained with either methyl green or hematoxylin.
T8924 35758-35764 JJ denotes Serial
T8926 35765-35766 CD denotes 4
T8928 35766-35767 HYPH denotes -
T8927 35767-35769 NN denotes μm
T8925 35770-35778 NNS denotes sections
T8930 35779-35783 VBD denotes were
T8929 35784-35787 VBN denotes cut
T8931 35788-35790 IN denotes on
T8932 35791-35792 DT denotes a
T8934 35793-35801 VBN denotes modified
T8935 35802-35807 NNP denotes Leica
T8936 35808-35810 NNP denotes RM
T8937 35811-35815 CD denotes 2155
T8938 35816-35822 JJ denotes rotary
T8933 35823-35832 NN denotes microtome
T8939 35833-35834 -LRB- denotes (
T8941 35834-35839 NNP denotes Leica
T8940 35840-35852 NNP denotes Microsystems
T8942 35852-35854 , denotes ,
T8943 35854-35862 NNP denotes Richmond
T8944 35863-35867 NNP denotes Hill
T8945 35867-35869 , denotes ,
T8946 35869-35876 NNP denotes Ontario
T8947 35876-35878 , denotes ,
T8948 35878-35884 NNP denotes Canada
T8949 35884-35885 -RRB- denotes )
T8950 35885-35887 , denotes ,
T8951 35887-35894 VBN denotes stained
T8952 35895-35899 IN denotes with
T8953 35900-35901 DT denotes a
T8955 35902-35903 CD denotes 1
T8956 35903-35904 SYM denotes :
T8957 35904-35907 CD denotes 600
T8954 35908-35916 NN denotes dilution
T8958 35917-35919 IN denotes of
T8959 35920-35923 DT denotes the
T8961 35924-35927 NN denotes AD1
T8962 35928-35938 JJ denotes anti-Sam68
T8960 35939-35947 NN denotes antibody
T8963 35948-35949 -LRB- denotes [
T8964 35949-35951 CD denotes 46
T8965 35951-35952 -RRB- denotes ]
T8966 35953-35956 CC denotes and
T8967 35957-35971 VBN denotes counterstained
T8968 35972-35976 IN denotes with
T8969 35977-35983 CC denotes either
T8971 35984-35990 NN denotes methyl
T8970 35991-35996 NN denotes green
T8972 35997-35999 CC denotes or
T8973 36000-36011 NN denotes hematoxylin
T8974 36011-36012 . denotes .
T8975 36012-36189 sentence denotes Adult mice were given an intraperitoneal injection of 30 mg/kg calcein at 7 days and 30 mg/kg tetracycline at 2 days prior to sacrifice to label actively mineralizing surfaces.
T8976 36013-36018 JJ denotes Adult
T8977 36019-36023 NNS denotes mice
T8979 36024-36028 VBD denotes were
T8978 36029-36034 VBN denotes given
T8980 36035-36037 DT denotes an
T8982 36038-36053 JJ denotes intraperitoneal
T8981 36054-36063 NN denotes injection
T8983 36064-36066 IN denotes of
T8984 36067-36069 CD denotes 30
T8985 36070-36072 NN denotes mg
T8987 36072-36073 SYM denotes /
T8988 36073-36075 NN denotes kg
T8986 36076-36083 NN denotes calcein
T8989 36084-36086 IN denotes at
T8990 36087-36088 CD denotes 7
T8991 36089-36093 NNS denotes days
T8992 36094-36097 CC denotes and
T8993 36098-36100 CD denotes 30
T8994 36101-36103 NN denotes mg
T8996 36103-36104 SYM denotes /
T8997 36104-36106 NN denotes kg
T8995 36107-36119 NN denotes tetracycline
T8998 36120-36122 IN denotes at
T8999 36123-36124 CD denotes 2
T9000 36125-36129 NNS denotes days
T9001 36130-36135 JJ denotes prior
T9002 36136-36138 IN denotes to
T9003 36139-36148 NN denotes sacrifice
T9004 36149-36151 TO denotes to
T9005 36152-36157 VB denotes label
T9006 36158-36166 RB denotes actively
T9007 36167-36179 VBG denotes mineralizing
T9008 36180-36188 NNS denotes surfaces
T9009 36188-36189 . denotes .
T9010 36189-36386 sentence denotes After overnight fixation in 4% paraformaldehyde and rinsing in PBS, the left femur and tibia were embedded in polymethylmethacrylate (MMA) or a mixture of 50% MMA and 50% glycolmethacrylate (GMA).
T9011 36190-36195 IN denotes After
T9013 36196-36205 RB denotes overnight
T9014 36206-36214 NN denotes fixation
T9015 36215-36217 IN denotes in
T9016 36218-36219 CD denotes 4
T9017 36219-36220 NN denotes %
T9018 36221-36237 NN denotes paraformaldehyde
T9019 36238-36241 CC denotes and
T9020 36242-36249 VBG denotes rinsing
T9021 36250-36252 IN denotes in
T9022 36253-36256 NN denotes PBS
T9023 36256-36258 , denotes ,
T9024 36258-36261 DT denotes the
T9026 36262-36266 JJ denotes left
T9025 36267-36272 NN denotes femur
T9027 36273-36276 CC denotes and
T9028 36277-36282 NN denotes tibia
T9029 36283-36287 VBD denotes were
T9012 36288-36296 VBN denotes embedded
T9030 36297-36299 IN denotes in
T9031 36300-36322 NN denotes polymethylmethacrylate
T9032 36323-36324 -LRB- denotes (
T9033 36324-36327 NN denotes MMA
T9034 36327-36328 -RRB- denotes )
T9035 36329-36331 CC denotes or
T9036 36332-36333 DT denotes a
T9037 36334-36341 NN denotes mixture
T9038 36342-36344 IN denotes of
T9039 36345-36347 CD denotes 50
T9040 36347-36348 NN denotes %
T9041 36349-36352 NN denotes MMA
T9042 36353-36356 CC denotes and
T9043 36357-36359 CD denotes 50
T9044 36359-36360 NN denotes %
T9045 36361-36379 NN denotes glycolmethacrylate
T9046 36380-36381 -LRB- denotes (
T9047 36381-36384 NN denotes GMA
T9048 36384-36385 -RRB- denotes )
T9049 36385-36386 . denotes .
T9050 36386-36600 sentence denotes Serial 4- to 6-μm sections of MMA-embedded tissues were left unstained or stained with von Kossa and toluidine blue or with toluidine blue alone, while 4-μm MMA-GMA sections were stained for TRAP and ALP activity.
T9051 36387-36393 JJ denotes Serial
T9053 36394-36395 CD denotes 4
T9055 36395-36396 HYPH denotes -
T9056 36397-36399 IN denotes to
T9054 36400-36401 CD denotes 6
T9058 36401-36402 HYPH denotes -
T9057 36402-36404 NN denotes μm
T9052 36405-36413 NNS denotes sections
T9060 36414-36416 IN denotes of
T9061 36417-36420 NN denotes MMA
T9063 36420-36421 HYPH denotes -
T9062 36421-36429 VBN denotes embedded
T9064 36430-36437 NNS denotes tissues
T9065 36438-36442 VBD denotes were
T9059 36443-36447 VBN denotes left
T9066 36448-36457 JJ denotes unstained
T9067 36458-36460 CC denotes or
T9068 36461-36468 VBN denotes stained
T9069 36469-36473 IN denotes with
T9070 36474-36477 NNP denotes von
T9071 36478-36483 NNP denotes Kossa
T9072 36484-36487 CC denotes and
T9073 36488-36497 NN denotes toluidine
T9074 36498-36502 NN denotes blue
T9075 36503-36505 CC denotes or
T9076 36506-36510 IN denotes with
T9077 36511-36520 NN denotes toluidine
T9078 36521-36525 NN denotes blue
T9079 36526-36531 RB denotes alone
T9080 36531-36533 , denotes ,
T9081 36533-36538 IN denotes while
T9083 36539-36540 CD denotes 4
T9085 36540-36541 HYPH denotes -
T9084 36541-36543 NN denotes μm
T9087 36544-36547 NN denotes MMA
T9089 36547-36548 HYPH denotes -
T9088 36548-36551 NN denotes GMA
T9086 36552-36560 NNS denotes sections
T9090 36561-36565 VBD denotes were
T9082 36566-36573 VBN denotes stained
T9091 36574-36577 IN denotes for
T9092 36578-36582 NN denotes TRAP
T9094 36583-36586 CC denotes and
T9095 36587-36590 NN denotes ALP
T9093 36591-36599 NN denotes activity
T9096 36599-36600 . denotes .
T9097 36600-36919 sentence denotes Images were captured using a Leica DMR microscope (Leica Microsystems) equipped with a Retiga 1300 camera (Qimaging, Burnaby, British Columbia, Canada) and the primary histomorphometric data obtained using Bioquant Nova Prime image analysis software (Bioquant Image Analysis Corp, Nashville, Tennessee, United States).
T9098 36601-36607 NNS denotes Images
T9100 36608-36612 VBD denotes were
T9099 36613-36621 VBN denotes captured
T9101 36622-36627 VBG denotes using
T9102 36628-36629 DT denotes a
T9104 36630-36635 NNP denotes Leica
T9105 36636-36639 NN denotes DMR
T9103 36640-36650 NN denotes microscope
T9106 36651-36652 -LRB- denotes (
T9108 36652-36657 NNP denotes Leica
T9107 36658-36670 NNP denotes Microsystems
T9109 36670-36671 -RRB- denotes )
T9110 36672-36680 VBN denotes equipped
T9111 36681-36685 IN denotes with
T9112 36686-36687 DT denotes a
T9114 36688-36694 NNP denotes Retiga
T9115 36695-36699 CD denotes 1300
T9113 36700-36706 NN denotes camera
T9116 36707-36708 -LRB- denotes (
T9117 36708-36716 NNP denotes Qimaging
T9118 36716-36718 , denotes ,
T9119 36718-36725 NNP denotes Burnaby
T9120 36725-36727 , denotes ,
T9121 36727-36734 NNP denotes British
T9122 36735-36743 NNP denotes Columbia
T9123 36743-36745 , denotes ,
T9124 36745-36751 NNP denotes Canada
T9125 36751-36752 -RRB- denotes )
T9126 36753-36756 CC denotes and
T9127 36757-36760 DT denotes the
T9129 36761-36768 JJ denotes primary
T9130 36769-36786 JJ denotes histomorphometric
T9128 36787-36791 NNS denotes data
T9131 36792-36800 VBN denotes obtained
T9132 36801-36806 VBG denotes using
T9133 36807-36815 NNP denotes Bioquant
T9135 36816-36820 NNP denotes Nova
T9134 36821-36826 NNP denotes Prime
T9137 36827-36832 NN denotes image
T9138 36833-36841 NN denotes analysis
T9136 36842-36850 NN denotes software
T9139 36851-36852 -LRB- denotes (
T9141 36852-36860 NNP denotes Bioquant
T9142 36861-36866 NNP denotes Image
T9143 36867-36875 NNP denotes Analysis
T9140 36876-36880 NNP denotes Corp
T9144 36880-36882 , denotes ,
T9145 36882-36891 NNP denotes Nashville
T9146 36891-36893 , denotes ,
T9147 36893-36902 NNP denotes Tennessee
T9148 36902-36904 , denotes ,
T9149 36904-36910 NNP denotes United
T9150 36911-36917 NNP denotes States
T9151 36917-36918 -RRB- denotes )
T9152 36918-36919 . denotes .
T9153 36919-37039 sentence denotes Nomenclature and abbreviations conform to those recommended by the American Society for Bone and Mineral Research [50].
T9154 36920-36932 NN denotes Nomenclature
T9156 36933-36936 CC denotes and
T9157 36937-36950 NNS denotes abbreviations
T9155 36951-36958 VBP denotes conform
T9158 36959-36961 IN denotes to
T9159 36962-36967 DT denotes those
T9160 36968-36979 VBN denotes recommended
T9161 36980-36982 IN denotes by
T9162 36983-36986 DT denotes the
T9164 36987-36995 NNP denotes American
T9163 36996-37003 NNP denotes Society
T9165 37004-37007 IN denotes for
T9166 37008-37012 NNP denotes Bone
T9168 37013-37016 CC denotes and
T9169 37017-37024 NNP denotes Mineral
T9167 37025-37033 NNP denotes Research
T9170 37034-37035 -LRB- denotes [
T9171 37035-37037 CD denotes 50
T9172 37037-37038 -RRB- denotes ]
T9173 37038-37039 . denotes .
T9293 37041-37051 NN denotes Generation
T9294 37052-37054 IN denotes of
T9295 37055-37059 NNS denotes mice
T9296 37060-37064 IN denotes with
T9297 37065-37073 VBN denotes targeted
T9298 37074-37084 NN denotes disruption
T9299 37085-37087 IN denotes of
T9300 37088-37091 DT denotes the
T9302 37092-37097 NN denotes sam68
T9301 37098-37102 NN denotes gene
T9303 37102-37103 . denotes .
T9304 37103-37244 sentence denotes A λ bacteriophage clone encompassing Sam68 exons 3 to 9 was isolated from a 129/SvJ genomic library using full-length Sam68 cDNA as a probe.
T9305 37104-37105 DT denotes A
T9307 37106-37107 NN denotes λ
T9308 37108-37121 NN denotes bacteriophage
T9306 37122-37127 NN denotes clone
T9310 37128-37140 VBG denotes encompassing
T9311 37141-37146 NN denotes Sam68
T9313 37147-37152 NNS denotes exons
T9312 37153-37154 CD denotes 3
T9314 37155-37157 IN denotes to
T9315 37158-37159 CD denotes 9
T9316 37160-37163 VBD denotes was
T9309 37164-37172 VBN denotes isolated
T9317 37173-37177 IN denotes from
T9318 37178-37179 DT denotes a
T9320 37180-37183 CD denotes 129
T9322 37183-37184 HYPH denotes /
T9321 37184-37187 NN denotes SvJ
T9323 37188-37195 JJ denotes genomic
T9319 37196-37203 NN denotes library
T9324 37204-37209 VBG denotes using
T9325 37210-37214 JJ denotes full
T9327 37214-37215 HYPH denotes -
T9326 37215-37221 NN denotes length
T9329 37222-37227 NN denotes Sam68
T9328 37228-37232 NN denotes cDNA
T9330 37233-37235 IN denotes as
T9331 37236-37237 DT denotes a
T9332 37238-37243 NN denotes probe
T9333 37243-37244 . denotes .
T9334 37244-37454 sentence denotes XbaI-digested Sam68 genomic DNA fragments of 4 kb (encompassing exon 4 and part of exon 5) and 3kb (spanning part of exon 5 and exon 6) were subcloned in Bluescript SK resulting in pBS4 and pBS3, respectively.
T9335 37245-37249 NN denotes XbaI
T9337 37249-37250 HYPH denotes -
T9336 37250-37258 VBN denotes digested
T9339 37259-37264 NN denotes Sam68
T9340 37265-37272 JJ denotes genomic
T9341 37273-37276 NN denotes DNA
T9338 37277-37286 NNS denotes fragments
T9343 37287-37289 IN denotes of
T9344 37290-37291 CD denotes 4
T9345 37292-37294 NN denotes kb
T9346 37295-37296 -LRB- denotes (
T9347 37296-37308 VBG denotes encompassing
T9348 37309-37313 NN denotes exon
T9349 37314-37315 CD denotes 4
T9350 37316-37319 CC denotes and
T9351 37320-37324 NN denotes part
T9352 37325-37327 IN denotes of
T9353 37328-37332 NN denotes exon
T9354 37333-37334 CD denotes 5
T9355 37334-37335 -RRB- denotes )
T9356 37336-37339 CC denotes and
T9357 37340-37343 NN denotes 3kb
T9358 37344-37345 -LRB- denotes (
T9359 37345-37353 VBG denotes spanning
T9360 37354-37358 NN denotes part
T9361 37359-37361 IN denotes of
T9362 37362-37366 NN denotes exon
T9363 37367-37368 CD denotes 5
T9364 37369-37372 CC denotes and
T9365 37373-37377 NN denotes exon
T9366 37378-37379 CD denotes 6
T9367 37379-37380 -RRB- denotes )
T9368 37381-37385 VBD denotes were
T9342 37386-37395 VBN denotes subcloned
T9369 37396-37398 IN denotes in
T9370 37399-37409 NN denotes Bluescript
T9371 37410-37412 NN denotes SK
T9372 37413-37422 VBG denotes resulting
T9373 37423-37425 IN denotes in
T9374 37426-37430 NN denotes pBS4
T9375 37431-37434 CC denotes and
T9376 37435-37439 NN denotes pBS3
T9377 37439-37441 , denotes ,
T9378 37441-37453 RB denotes respectively
T9379 37453-37454 . denotes .
T9380 37454-37601 sentence denotes A DNA fragment was amplified from pBS4 with the following oligonucleotides (5′-AAT GTC TAG AAA CAA CTC ATA TAC AGA C-3′) and the universal primer.
T9381 37455-37456 DT denotes A
T9383 37457-37460 NN denotes DNA
T9382 37461-37469 NN denotes fragment
T9385 37470-37473 VBD denotes was
T9384 37474-37483 VBN denotes amplified
T9386 37484-37488 IN denotes from
T9387 37489-37493 NN denotes pBS4
T9388 37494-37498 IN denotes with
T9389 37499-37502 DT denotes the
T9391 37503-37512 JJ denotes following
T9390 37513-37529 NNS denotes oligonucleotides
T9392 37530-37531 -LRB- denotes (
T9393 37531-37532 CD denotes 5
T9394 37532-37533 SYM denotes ′
T9395 37533-37534 HYPH denotes -
T9396 37534-37537 NN denotes AAT
T9398 37538-37541 NN denotes GTC
T9399 37542-37545 NN denotes TAG
T9400 37546-37549 NN denotes AAA
T9401 37550-37553 NN denotes CAA
T9402 37554-37557 NN denotes CTC
T9403 37558-37561 NN denotes ATA
T9404 37562-37565 NN denotes TAC
T9405 37566-37569 NN denotes AGA
T9397 37570-37571 NN denotes C
T9406 37571-37572 HYPH denotes -
T9407 37572-37573 CD denotes 3
T9408 37573-37574 SYM denotes ′
T9409 37574-37575 -RRB- denotes )
T9410 37576-37579 CC denotes and
T9411 37580-37583 DT denotes the
T9413 37584-37593 JJ denotes universal
T9412 37594-37600 NN denotes primer
T9414 37600-37601 . denotes .
T9415 37601-37752 sentence denotes The XbaI-digested 1-kb DNA fragment was subcloned in the XbaI site of pPNT (a gift from Andrew Karaplis, McGill University, Montréal, Quebec, Canada).
T9416 37602-37605 DT denotes The
T9418 37606-37610 NN denotes XbaI
T9420 37610-37611 HYPH denotes -
T9419 37611-37619 VBN denotes digested
T9421 37620-37621 CD denotes 1
T9423 37621-37622 HYPH denotes -
T9422 37622-37624 NN denotes kb
T9424 37625-37628 NN denotes DNA
T9417 37629-37637 NN denotes fragment
T9426 37638-37641 VBD denotes was
T9425 37642-37651 VBN denotes subcloned
T9427 37652-37654 IN denotes in
T9428 37655-37658 DT denotes the
T9430 37659-37663 NN denotes XbaI
T9429 37664-37668 NN denotes site
T9431 37669-37671 IN denotes of
T9432 37672-37676 NN denotes pPNT
T9433 37677-37678 -LRB- denotes (
T9435 37678-37679 DT denotes a
T9434 37680-37684 NN denotes gift
T9436 37685-37689 IN denotes from
T9437 37690-37696 NNP denotes Andrew
T9438 37697-37705 NNP denotes Karaplis
T9439 37705-37707 , denotes ,
T9440 37707-37713 NNP denotes McGill
T9441 37714-37724 NNP denotes University
T9442 37724-37726 , denotes ,
T9443 37726-37734 NNP denotes Montréal
T9444 37734-37736 , denotes ,
T9445 37736-37742 NNP denotes Quebec
T9446 37742-37744 , denotes ,
T9447 37744-37750 NNP denotes Canada
T9448 37750-37751 -RRB- denotes )
T9449 37751-37752 . denotes .
T9450 37752-37966 sentence denotes The 3-kb fragment from pBS3 was amplified by PCR with (5′-GGG ATG CGG CCG CTC TAG AAT TGT CCT ACT TGA ACG G-3') and (5′-CGG TGG CGG CCG CTG TCG ACC TGA GTA ACA TTT CTT A-3′) and subcloned in the NotI site of pPNT.
T9451 37753-37756 DT denotes The
T9453 37757-37758 CD denotes 3
T9455 37758-37759 HYPH denotes -
T9454 37759-37761 NN denotes kb
T9452 37762-37770 NN denotes fragment
T9457 37771-37775 IN denotes from
T9458 37776-37780 NN denotes pBS3
T9459 37781-37784 VBD denotes was
T9456 37785-37794 VBN denotes amplified
T9460 37795-37797 IN denotes by
T9461 37798-37801 NN denotes PCR
T9462 37802-37806 IN denotes with
T9463 37807-37808 -LRB- denotes (
T9464 37808-37809 CD denotes 5
T9465 37809-37810 SYM denotes ′
T9466 37810-37811 HYPH denotes -
T9467 37811-37814 NN denotes GGG
T9469 37815-37818 NN denotes ATG
T9470 37819-37822 NN denotes CGG
T9471 37823-37826 NN denotes CCG
T9472 37827-37830 NN denotes CTC
T9473 37831-37834 NN denotes TAG
T9474 37835-37838 NN denotes AAT
T9475 37839-37842 NN denotes TGT
T9476 37843-37846 NN denotes CCT
T9477 37847-37850 NN denotes ACT
T9478 37851-37854 NN denotes TGA
T9479 37855-37858 NN denotes ACG
T9468 37859-37860 NN denotes G
T9480 37860-37861 HYPH denotes -
T9481 37861-37862 CD denotes 3
T9482 37862-37863 SYM denotes '
T9483 37863-37864 -RRB- denotes )
T9484 37865-37868 CC denotes and
T9485 37869-37870 -LRB- denotes (
T9486 37870-37871 CD denotes 5
T9487 37871-37872 SYM denotes ′
T9488 37872-37873 HYPH denotes -
T9489 37873-37876 NN denotes CGG
T9491 37877-37880 NN denotes TGG
T9492 37881-37884 NN denotes CGG
T9493 37885-37888 NN denotes CCG
T9494 37889-37892 NN denotes CTG
T9495 37893-37896 NN denotes TCG
T9496 37897-37900 NN denotes ACC
T9497 37901-37904 NN denotes TGA
T9498 37905-37908 NN denotes GTA
T9499 37909-37912 NN denotes ACA
T9500 37913-37916 NN denotes TTT
T9501 37917-37920 NN denotes CTT
T9490 37921-37922 NN denotes A
T9502 37922-37923 HYPH denotes -
T9503 37923-37924 CD denotes 3
T9504 37924-37925 SYM denotes ′
T9505 37925-37926 -RRB- denotes )
T9506 37927-37930 CC denotes and
T9507 37931-37940 VBN denotes subcloned
T9508 37941-37943 IN denotes in
T9509 37944-37947 DT denotes the
T9511 37948-37952 NN denotes NotI
T9510 37953-37957 NN denotes site
T9512 37958-37960 IN denotes of
T9513 37961-37965 NN denotes pPNT
T9514 37965-37966 . denotes .
T9515 37966-38074 sentence denotes The targeting vector pPNT-Sam68 replaces exon 4 and part of exon 5 with a neomycin-resistant gene cassette.
T9516 37967-37970 DT denotes The
T9518 37971-37980 NN denotes targeting
T9517 37981-37987 NN denotes vector
T9520 37988-37992 NN denotes pPNT
T9522 37992-37993 HYPH denotes -
T9521 37993-37998 NN denotes Sam68
T9519 37999-38007 VBZ denotes replaces
T9523 38008-38012 NN denotes exon
T9524 38013-38014 CD denotes 4
T9525 38015-38018 CC denotes and
T9526 38019-38023 NN denotes part
T9527 38024-38026 IN denotes of
T9528 38027-38031 NN denotes exon
T9529 38032-38033 CD denotes 5
T9530 38034-38038 IN denotes with
T9531 38039-38040 DT denotes a
T9533 38041-38049 NN denotes neomycin
T9535 38049-38050 HYPH denotes -
T9534 38050-38059 JJ denotes resistant
T9536 38060-38064 NN denotes gene
T9532 38065-38073 NN denotes cassette
T9537 38073-38074 . denotes .
T9538 38074-38231 sentence denotes An SalI site was introduced at the 3′ end of the 3-kb DNA fragment and was used to linearize the plasmid for electroporation into embryonic stem (ES) cells.
T9539 38075-38077 DT denotes An
T9541 38078-38082 NN denotes SalI
T9540 38083-38087 NN denotes site
T9543 38088-38091 VBD denotes was
T9542 38092-38102 VBN denotes introduced
T9544 38103-38105 IN denotes at
T9545 38106-38109 DT denotes the
T9547 38110-38111 CD denotes 3
T9548 38111-38112 SYM denotes ′
T9546 38113-38116 NN denotes end
T9549 38117-38119 IN denotes of
T9550 38120-38123 DT denotes the
T9552 38124-38125 CD denotes 3
T9554 38125-38126 HYPH denotes -
T9553 38126-38128 NN denotes kb
T9555 38129-38132 NN denotes DNA
T9551 38133-38141 NN denotes fragment
T9556 38142-38145 CC denotes and
T9557 38146-38149 VBD denotes was
T9558 38150-38154 VBN denotes used
T9559 38155-38157 TO denotes to
T9560 38158-38167 VB denotes linearize
T9561 38168-38171 DT denotes the
T9562 38172-38179 NN denotes plasmid
T9563 38180-38183 IN denotes for
T9564 38184-38199 NN denotes electroporation
T9565 38200-38204 IN denotes into
T9566 38205-38214 JJ denotes embryonic
T9567 38215-38219 NN denotes stem
T9569 38220-38221 -LRB- denotes (
T9570 38221-38223 NN denotes ES
T9571 38223-38224 -RRB- denotes )
T9568 38225-38230 NNS denotes cells
T9572 38230-38231 . denotes .
T9573 38231-38384 sentence denotes Approximately 1,000 ES colonies were screened and two clones were identified that contained the Sam68 mutant allele, as determined by Southern blotting.
T9574 38232-38245 RB denotes Approximately
T9575 38246-38251 CD denotes 1,000
T9577 38252-38254 NN denotes ES
T9576 38255-38263 NNS denotes colonies
T9579 38264-38268 VBD denotes were
T9578 38269-38277 VBN denotes screened
T9580 38278-38281 CC denotes and
T9581 38282-38285 CD denotes two
T9582 38286-38292 NNS denotes clones
T9584 38293-38297 VBD denotes were
T9583 38298-38308 VBN denotes identified
T9585 38309-38313 WDT denotes that
T9586 38314-38323 VBD denotes contained
T9587 38324-38327 DT denotes the
T9588 38328-38333 NN denotes Sam68
T9590 38334-38340 NN denotes mutant
T9589 38341-38347 NN denotes allele
T9591 38347-38349 , denotes ,
T9592 38349-38351 IN denotes as
T9593 38352-38362 VBN denotes determined
T9594 38363-38365 IN denotes by
T9595 38366-38374 NNP denotes Southern
T9596 38375-38383 NN denotes blotting
T9597 38383-38384 . denotes .
T9598 38384-38577 sentence denotes Targeted ES cells were injected into 3.5-day-old BALB/c blastocysts and were transferred into CD-1 foster mothers, and animals classified as chimeras by coat color were mated with BALB/c mice.
T9599 38385-38393 VBN denotes Targeted
T9601 38394-38396 NN denotes ES
T9600 38397-38402 NNS denotes cells
T9603 38403-38407 VBD denotes were
T9602 38408-38416 VBN denotes injected
T9604 38417-38421 IN denotes into
T9605 38422-38425 CD denotes 3.5
T9607 38425-38426 HYPH denotes -
T9606 38426-38429 NN denotes day
T9609 38429-38430 HYPH denotes -
T9608 38430-38433 JJ denotes old
T9611 38434-38438 NN denotes BALB
T9613 38438-38439 HYPH denotes /
T9612 38439-38440 NN denotes c
T9610 38441-38452 NNS denotes blastocysts
T9614 38453-38456 CC denotes and
T9615 38457-38461 VBD denotes were
T9616 38462-38473 VBN denotes transferred
T9617 38474-38478 IN denotes into
T9618 38479-38481 NN denotes CD
T9620 38481-38482 HYPH denotes -
T9621 38482-38483 CD denotes 1
T9622 38484-38490 NN denotes foster
T9619 38491-38498 NNS denotes mothers
T9623 38498-38500 , denotes ,
T9624 38500-38503 CC denotes and
T9625 38504-38511 NNS denotes animals
T9627 38512-38522 VBN denotes classified
T9628 38523-38525 IN denotes as
T9629 38526-38534 NNS denotes chimeras
T9630 38535-38537 IN denotes by
T9631 38538-38542 NN denotes coat
T9632 38543-38548 NN denotes color
T9633 38549-38553 VBD denotes were
T9626 38554-38559 VBN denotes mated
T9634 38560-38564 IN denotes with
T9635 38565-38569 NN denotes BALB
T9637 38569-38570 HYPH denotes /
T9636 38570-38571 NN denotes c
T9638 38572-38576 NNS denotes mice
T9639 38576-38577 . denotes .
T9640 38577-38665 sentence denotes Germ line transmission was achieved and the mice were maintained in C57BL/6 background.
T9641 38578-38582 NN denotes Germ
T9642 38583-38587 NN denotes line
T9643 38588-38600 NN denotes transmission
T9645 38601-38604 VBD denotes was
T9644 38605-38613 VBN denotes achieved
T9646 38614-38617 CC denotes and
T9647 38618-38621 DT denotes the
T9648 38622-38626 NNS denotes mice
T9650 38627-38631 VBD denotes were
T9649 38632-38642 VBN denotes maintained
T9651 38643-38645 IN denotes in
T9652 38646-38651 NN denotes C57BL
T9654 38651-38652 HYPH denotes /
T9655 38652-38653 CD denotes 6
T9653 38654-38664 NN denotes background
T9656 38664-38665 . denotes .
T9657 38665-38871 sentence denotes The mice used for this study represent mice that were backcrossed in C57BL/6 between three and eight generations; in addition, we maintained the mice in the 129/SvJ strain and observed a similar phenotype.
T9658 38666-38669 DT denotes The
T9659 38670-38674 NNS denotes mice
T9661 38675-38679 VBN denotes used
T9662 38680-38683 IN denotes for
T9663 38684-38688 DT denotes this
T9664 38689-38694 NN denotes study
T9660 38695-38704 VBP denotes represent
T9666 38705-38709 NNS denotes mice
T9667 38710-38714 WDT denotes that
T9669 38715-38719 VBD denotes were
T9668 38720-38731 VBN denotes backcrossed
T9670 38732-38734 IN denotes in
T9671 38735-38740 NN denotes C57BL
T9672 38740-38741 HYPH denotes /
T9673 38741-38742 CD denotes 6
T9674 38743-38750 IN denotes between
T9675 38751-38756 CD denotes three
T9677 38757-38760 CC denotes and
T9678 38761-38766 CD denotes eight
T9676 38767-38778 NNS denotes generations
T9679 38778-38779 : denotes ;
T9680 38780-38782 IN denotes in
T9681 38783-38791 NN denotes addition
T9682 38791-38793 , denotes ,
T9683 38793-38795 PRP denotes we
T9665 38796-38806 VBD denotes maintained
T9684 38807-38810 DT denotes the
T9685 38811-38815 NNS denotes mice
T9686 38816-38818 IN denotes in
T9687 38819-38822 DT denotes the
T9689 38823-38826 CD denotes 129
T9691 38826-38827 HYPH denotes /
T9690 38827-38830 NN denotes SvJ
T9688 38831-38837 NN denotes strain
T9692 38838-38841 CC denotes and
T9693 38842-38850 VBD denotes observed
T9694 38851-38852 DT denotes a
T9696 38853-38860 JJ denotes similar
T9695 38861-38870 NN denotes phenotype
T9697 38870-38871 . denotes .
T9732 38873-38883 NN denotes Genotyping
T9733 38884-38887 CC denotes and
T9734 38888-38898 NN denotes immunoblot
T9735 38899-38907 NNS denotes analyses
T9736 38907-38908 . denotes .
T9737 38908-39047 sentence denotes All mouse procedures were performed in accordance with McGill University guidelines, which are set by the Canadian Council on Animal Care.
T9738 38909-38912 DT denotes All
T9740 38913-38918 NN denotes mouse
T9739 38919-38929 NNS denotes procedures
T9742 38930-38934 VBD denotes were
T9741 38935-38944 VBN denotes performed
T9743 38945-38947 IN denotes in
T9744 38948-38958 NN denotes accordance
T9745 38959-38963 IN denotes with
T9746 38964-38970 NNP denotes McGill
T9747 38971-38981 NNP denotes University
T9748 38982-38992 NNS denotes guidelines
T9749 38992-38994 , denotes ,
T9750 38994-38999 WDT denotes which
T9752 39000-39003 VBP denotes are
T9751 39004-39007 VBN denotes set
T9753 39008-39010 IN denotes by
T9754 39011-39014 DT denotes the
T9756 39015-39023 NNP denotes Canadian
T9755 39024-39031 NNP denotes Council
T9757 39032-39034 IN denotes on
T9758 39035-39041 NNP denotes Animal
T9759 39042-39046 NNP denotes Care
T9760 39046-39047 . denotes .
T9761 39047-39151 sentence denotes Genomic DNA was isolated from tail biopsies and analyzed by Southern blotting and genomic PCR analysis.
T9762 39048-39055 JJ denotes Genomic
T9763 39056-39059 NN denotes DNA
T9765 39060-39063 VBD denotes was
T9764 39064-39072 VBN denotes isolated
T9766 39073-39077 IN denotes from
T9767 39078-39082 NN denotes tail
T9768 39083-39091 NNS denotes biopsies
T9769 39092-39095 CC denotes and
T9770 39096-39104 VBN denotes analyzed
T9771 39105-39107 IN denotes by
T9772 39108-39116 NNP denotes Southern
T9773 39117-39125 NN denotes blotting
T9774 39126-39129 CC denotes and
T9775 39130-39137 JJ denotes genomic
T9777 39138-39141 NN denotes PCR
T9776 39142-39150 NN denotes analysis
T9778 39150-39151 . denotes .
T9779 39151-39353 sentence denotes The DNA fragment utilized as the probe for the Southern blotting analysis was amplified with the following two oligonucleotides (5′-AAG CCT TTA CTG GTT GTG T-3′) and (5′-CTT GAA ACG CAC CGT AGG CT-3′).
T9780 39152-39155 DT denotes The
T9782 39156-39159 NN denotes DNA
T9781 39160-39168 NN denotes fragment
T9784 39169-39177 VBN denotes utilized
T9785 39178-39180 IN denotes as
T9786 39181-39184 DT denotes the
T9787 39185-39190 NN denotes probe
T9788 39191-39194 IN denotes for
T9789 39195-39198 DT denotes the
T9791 39199-39207 NNP denotes Southern
T9792 39208-39216 NN denotes blotting
T9790 39217-39225 NN denotes analysis
T9793 39226-39229 VBD denotes was
T9783 39230-39239 VBN denotes amplified
T9794 39240-39244 IN denotes with
T9795 39245-39248 DT denotes the
T9797 39249-39258 VBG denotes following
T9798 39259-39262 CD denotes two
T9796 39263-39279 NNS denotes oligonucleotides
T9799 39280-39281 -LRB- denotes (
T9800 39281-39282 CD denotes 5
T9801 39282-39283 SYM denotes ′
T9802 39283-39284 HYPH denotes -
T9803 39284-39287 NN denotes AAG
T9805 39288-39291 NN denotes CCT
T9806 39292-39295 NN denotes TTA
T9807 39296-39299 NN denotes CTG
T9808 39300-39303 NN denotes GTT
T9809 39304-39307 NN denotes GTG
T9804 39308-39309 NN denotes T
T9810 39309-39310 HYPH denotes -
T9811 39310-39311 CD denotes 3
T9812 39311-39312 SYM denotes ′
T9813 39312-39313 -RRB- denotes )
T9814 39314-39317 CC denotes and
T9815 39318-39319 -LRB- denotes (
T9816 39319-39320 CD denotes 5
T9817 39320-39321 SYM denotes ′
T9818 39321-39322 HYPH denotes -
T9819 39322-39325 NN denotes CTT
T9821 39326-39329 NN denotes GAA
T9822 39330-39333 NN denotes ACG
T9823 39334-39337 NN denotes CAC
T9824 39338-39341 NN denotes CGT
T9825 39342-39345 NN denotes AGG
T9820 39346-39348 NN denotes CT
T9826 39348-39349 HYPH denotes -
T9827 39349-39350 CD denotes 3
T9828 39350-39351 SYM denotes ′
T9829 39351-39352 -RRB- denotes )
T9830 39352-39353 . denotes .
T9831 39353-39527 sentence denotes The wild-type sam68 allele was identified by genomic PCR using the following oligonucleotides 5′-AAA TCC TAA CCC TCC TCA GTC AG-3′ and 5′-GAT ATG ATG GAT GAT ATC TGT CAG-3′.
T9832 39354-39357 DT denotes The
T9834 39358-39362 JJ denotes wild
T9836 39362-39363 HYPH denotes -
T9835 39363-39367 NN denotes type
T9837 39368-39373 NN denotes sam68
T9833 39374-39380 NN denotes allele
T9839 39381-39384 VBD denotes was
T9838 39385-39395 VBN denotes identified
T9840 39396-39398 IN denotes by
T9841 39399-39406 JJ denotes genomic
T9842 39407-39410 NN denotes PCR
T9843 39411-39416 VBG denotes using
T9844 39417-39420 DT denotes the
T9846 39421-39430 VBG denotes following
T9845 39431-39447 NNS denotes oligonucleotides
T9847 39448-39449 CD denotes 5
T9848 39449-39450 SYM denotes ′
T9849 39450-39451 HYPH denotes -
T9850 39451-39454 NN denotes AAA
T9852 39455-39458 NN denotes TCC
T9853 39459-39462 NN denotes TAA
T9854 39463-39466 NN denotes CCC
T9855 39467-39470 NN denotes TCC
T9856 39471-39474 NN denotes TCA
T9857 39475-39478 NN denotes GTC
T9851 39479-39481 NN denotes AG
T9858 39481-39482 HYPH denotes -
T9859 39482-39483 CD denotes 3
T9860 39483-39484 SYM denotes ′
T9861 39485-39488 CC denotes and
T9862 39489-39490 CD denotes 5
T9863 39490-39491 SYM denotes ′
T9864 39491-39492 HYPH denotes -
T9865 39492-39495 NN denotes GAT
T9867 39496-39499 NN denotes ATG
T9868 39500-39503 NN denotes ATG
T9869 39504-39507 NN denotes GAT
T9870 39508-39511 NN denotes GAT
T9871 39512-39515 NN denotes ATC
T9872 39516-39519 NN denotes TGT
T9866 39520-39523 NN denotes CAG
T9873 39523-39524 HYPH denotes -
T9874 39524-39525 CD denotes 3
T9875 39525-39526 SYM denotes ′
T9876 39526-39527 . denotes .
T9877 39527-39695 sentence denotes The sam68-targeted allele was identified by genomic PCR using the following oligonucleotides 5′-CTT GGG TGG AGA GGC TAT TCG-3′ and 5′-GTC GGG CAT GCG CGC CTT GAG C-3′.
T9878 39528-39531 DT denotes The
T9880 39532-39537 NN denotes sam68
T9882 39537-39538 HYPH denotes -
T9881 39538-39546 VBN denotes targeted
T9879 39547-39553 NN denotes allele
T9884 39554-39557 VBD denotes was
T9883 39558-39568 VBN denotes identified
T9885 39569-39571 IN denotes by
T9886 39572-39579 JJ denotes genomic
T9887 39580-39583 NN denotes PCR
T9888 39584-39589 VBG denotes using
T9889 39590-39593 DT denotes the
T9891 39594-39603 VBG denotes following
T9890 39604-39620 NNS denotes oligonucleotides
T9892 39621-39622 CD denotes 5
T9893 39622-39623 SYM denotes ′
T9894 39623-39624 HYPH denotes -
T9895 39624-39627 NN denotes CTT
T9897 39628-39631 NN denotes GGG
T9898 39632-39635 NN denotes TGG
T9899 39636-39639 NN denotes AGA
T9900 39640-39643 NN denotes GGC
T9901 39644-39647 NN denotes TAT
T9896 39648-39651 NN denotes TCG
T9902 39651-39652 HYPH denotes -
T9903 39652-39653 CD denotes 3
T9904 39653-39654 SYM denotes ′
T9905 39655-39658 CC denotes and
T9906 39659-39660 CD denotes 5
T9907 39660-39661 SYM denotes ′
T9908 39661-39662 HYPH denotes -
T9909 39662-39665 NN denotes GTC
T9911 39666-39669 NN denotes GGG
T9912 39670-39673 NN denotes CAT
T9913 39674-39677 NN denotes GCG
T9914 39678-39681 NN denotes CGC
T9915 39682-39685 NN denotes CTT
T9916 39686-39689 NN denotes GAG
T9910 39690-39691 NN denotes C
T9917 39691-39692 HYPH denotes -
T9918 39692-39693 CD denotes 3
T9919 39693-39694 SYM denotes ′
T9920 39694-39695 . denotes .
T9978 39697-39706 NN denotes Radiology
T9979 39707-39710 CC denotes and
T9980 39711-39716 NN denotes serum
T9981 39717-39729 NN denotes biochemistry
T9982 39730-39732 IN denotes on
T9983 39733-39738 NN denotes Sam68
T9985 39738-39739 SYM denotes +
T9986 39739-39740 HYPH denotes /
T9987 39740-39741 SYM denotes +
T9988 39742-39745 CC denotes and
T9989 39746-39751 NN denotes Sam68
T9990 39751-39752 SYM denotes −
T9991 39752-39753 HYPH denotes /
T9992 39753-39754 SYM denotes −
T9984 39755-39759 NNS denotes mice
T9993 39759-39760 . denotes .
T9994 39760-39848 sentence denotes Radiography, BMD, and micro-CT were performed essentially as described previously [63].
T9995 39761-39772 NN denotes Radiography
T9997 39772-39774 , denotes ,
T9998 39774-39777 NN denotes BMD
T9999 39777-39779 , denotes ,
T10000 39779-39782 CC denotes and
T10001 39783-39791 NN denotes micro-CT
T10002 39792-39796 VBD denotes were
T9996 39797-39806 VBN denotes performed
T10003 39807-39818 RB denotes essentially
T10005 39819-39821 IN denotes as
T10004 39822-39831 VBN denotes described
T10006 39832-39842 RB denotes previously
T10007 39843-39844 -LRB- denotes [
T10008 39844-39846 CD denotes 63
T10009 39846-39847 -RRB- denotes ]
T10010 39847-39848 . denotes .
T10011 39848-40055 sentence denotes Mice were administered a lethal dose of anesthetic at the indicated times, exsanguinated, and imaged using a Faxitron MX20 equipped with an FPX-2 Imaging system (Dalsa Medoptics, Waterloo, Ontario, Canada).
T10012 39849-39853 NNS denotes Mice
T10014 39854-39858 VBD denotes were
T10013 39859-39871 VBN denotes administered
T10015 39872-39873 DT denotes a
T10017 39874-39880 JJ denotes lethal
T10016 39881-39885 NN denotes dose
T10018 39886-39888 IN denotes of
T10019 39889-39899 JJ denotes anesthetic
T10020 39900-39902 IN denotes at
T10021 39903-39906 DT denotes the
T10023 39907-39916 VBN denotes indicated
T10022 39917-39922 NNS denotes times
T10024 39922-39924 , denotes ,
T10025 39924-39937 VBN denotes exsanguinated
T10026 39937-39939 , denotes ,
T10027 39939-39942 CC denotes and
T10028 39943-39949 VBN denotes imaged
T10029 39950-39955 VBG denotes using
T10030 39956-39957 DT denotes a
T10032 39958-39966 NNP denotes Faxitron
T10031 39967-39971 NNP denotes MX20
T10033 39972-39980 VBN denotes equipped
T10034 39981-39985 IN denotes with
T10035 39986-39988 DT denotes an
T10037 39989-39992 NN denotes FPX
T10038 39992-39993 HYPH denotes -
T10039 39993-39994 CD denotes 2
T10040 39995-40002 NN denotes Imaging
T10036 40003-40009 NN denotes system
T10041 40010-40011 -LRB- denotes (
T10043 40011-40016 NNP denotes Dalsa
T10042 40017-40026 NNP denotes Medoptics
T10044 40026-40028 , denotes ,
T10045 40028-40036 NNP denotes Waterloo
T10046 40036-40038 , denotes ,
T10047 40038-40045 NNP denotes Ontario
T10048 40045-40047 , denotes ,
T10049 40047-40053 NNP denotes Canada
T10050 40053-40054 -RRB- denotes )
T10051 40054-40055 . denotes .
T10052 40055-40167 sentence denotes Body fat, BMC, and BMD were evaluated using a Lunar PixiMUS 1.46 (GE-Lunar, Madison, Wisconsin, United States).
T10053 40056-40060 NN denotes Body
T10054 40061-40064 NN denotes fat
T10056 40064-40066 , denotes ,
T10057 40066-40069 NN denotes BMC
T10058 40069-40071 , denotes ,
T10059 40071-40074 CC denotes and
T10060 40075-40078 NN denotes BMD
T10061 40079-40083 VBD denotes were
T10055 40084-40093 VBN denotes evaluated
T10062 40094-40099 VBG denotes using
T10063 40100-40101 DT denotes a
T10065 40102-40107 NNP denotes Lunar
T10064 40108-40115 NNP denotes PixiMUS
T10066 40116-40120 CD denotes 1.46
T10067 40121-40122 -LRB- denotes (
T10069 40122-40124 NNP denotes GE
T10070 40124-40125 HYPH denotes -
T10068 40125-40130 NNP denotes Lunar
T10071 40130-40132 , denotes ,
T10072 40132-40139 NNP denotes Madison
T10073 40139-40141 , denotes ,
T10074 40141-40150 NNP denotes Wisconsin
T10075 40150-40152 , denotes ,
T10076 40152-40158 NNP denotes United
T10077 40159-40165 NNP denotes States
T10078 40165-40166 -RRB- denotes )
T10079 40166-40167 . denotes .
T10080 40167-40308 sentence denotes Morphometric parameters were determined on anesthetized mice at the time of sacrifice by direct measurement or from the Faxitron radiograph.
T10081 40168-40180 JJ denotes Morphometric
T10082 40181-40191 NNS denotes parameters
T10084 40192-40196 VBD denotes were
T10083 40197-40207 VBN denotes determined
T10085 40208-40210 IN denotes on
T10086 40211-40223 VBN denotes anesthetized
T10087 40224-40228 NNS denotes mice
T10088 40229-40231 IN denotes at
T10089 40232-40235 DT denotes the
T10090 40236-40240 NN denotes time
T10091 40241-40243 IN denotes of
T10092 40244-40253 NN denotes sacrifice
T10093 40254-40256 IN denotes by
T10094 40257-40263 JJ denotes direct
T10095 40264-40275 NN denotes measurement
T10096 40276-40278 CC denotes or
T10097 40279-40283 IN denotes from
T10098 40284-40287 DT denotes the
T10100 40288-40296 NNP denotes Faxitron
T10099 40297-40307 NN denotes radiograph
T10101 40307-40308 . denotes .
T10102 40308-40453 sentence denotes Micro-CT was performed on the left femur and fourth lumbar vertebra after removal of soft tissues and overnight fixation in 4% paraformaldehyde.
T10103 40309-40317 NN denotes Micro-CT
T10105 40318-40321 VBD denotes was
T10104 40322-40331 VBN denotes performed
T10106 40332-40334 IN denotes on
T10107 40335-40338 DT denotes the
T10109 40339-40343 JJ denotes left
T10108 40344-40349 NN denotes femur
T10110 40350-40353 CC denotes and
T10111 40354-40360 JJ denotes fourth
T10113 40361-40367 NN denotes lumbar
T10112 40368-40376 NN denotes vertebra
T10114 40377-40382 IN denotes after
T10115 40383-40390 NN denotes removal
T10116 40391-40393 IN denotes of
T10117 40394-40398 JJ denotes soft
T10118 40399-40406 NNS denotes tissues
T10119 40407-40410 CC denotes and
T10120 40411-40420 JJ denotes overnight
T10121 40421-40429 NN denotes fixation
T10122 40430-40432 IN denotes in
T10123 40433-40434 CD denotes 4
T10124 40434-40435 NN denotes %
T10125 40436-40452 NN denotes paraformaldehyde
T10126 40452-40453 . denotes .
T10127 40453-40556 sentence denotes The distal metaphysis was scanned with a Skyscan 1072 micro-CT instrument (Skyscan, Antwerp, Belgium).
T10128 40454-40457 DT denotes The
T10130 40458-40464 JJ denotes distal
T10129 40465-40475 NN denotes metaphysis
T10132 40476-40479 VBD denotes was
T10131 40480-40487 VBN denotes scanned
T10133 40488-40492 IN denotes with
T10134 40493-40494 DT denotes a
T10136 40495-40502 NNP denotes Skyscan
T10137 40503-40507 CD denotes 1072
T10138 40508-40516 NN denotes micro-CT
T10135 40517-40527 NN denotes instrument
T10139 40528-40529 -LRB- denotes (
T10140 40529-40536 NNP denotes Skyscan
T10141 40536-40538 , denotes ,
T10142 40538-40545 NNP denotes Antwerp
T10143 40545-40547 , denotes ,
T10144 40547-40554 NNP denotes Belgium
T10145 40554-40555 -RRB- denotes )
T10146 40555-40556 . denotes .
T10147 40556-40646 sentence denotes Image acquisition was performed at 100 kV and 98 μA, with a 0.9° rotation between frames.
T10148 40557-40562 NN denotes Image
T10149 40563-40574 NN denotes acquisition
T10151 40575-40578 VBD denotes was
T10150 40579-40588 VBN denotes performed
T10152 40589-40591 IN denotes at
T10153 40592-40595 CD denotes 100
T10154 40596-40598 NN denotes kV
T10155 40599-40602 CC denotes and
T10156 40603-40605 CD denotes 98
T10157 40606-40608 NN denotes μA
T10158 40608-40610 , denotes ,
T10159 40610-40614 IN denotes with
T10160 40615-40616 DT denotes a
T10162 40617-40620 CD denotes 0.9
T10163 40620-40621 NN denotes °
T10161 40622-40630 NN denotes rotation
T10164 40631-40638 IN denotes between
T10165 40639-40645 NNS denotes frames
T10166 40645-40646 . denotes .
T10167 40646-40816 sentence denotes The two-dimensional images were used to generate three-dimensional reconstructions to obtain quantitative data with the 3D Creator software supplied with the instrument.
T10168 40647-40650 DT denotes The
T10170 40651-40654 CD denotes two
T10172 40654-40655 HYPH denotes -
T10171 40655-40666 JJ denotes dimensional
T10169 40667-40673 NNS denotes images
T10174 40674-40678 VBD denotes were
T10173 40679-40683 VBN denotes used
T10175 40684-40686 TO denotes to
T10176 40687-40695 VB denotes generate
T10177 40696-40701 CD denotes three
T10179 40701-40702 HYPH denotes -
T10178 40702-40713 JJ denotes dimensional
T10180 40714-40729 NNS denotes reconstructions
T10181 40730-40732 TO denotes to
T10182 40733-40739 VB denotes obtain
T10183 40740-40752 JJ denotes quantitative
T10184 40753-40757 NNS denotes data
T10185 40758-40762 IN denotes with
T10186 40763-40766 DT denotes the
T10188 40767-40769 JJ denotes 3D
T10189 40770-40777 NN denotes Creator
T10187 40778-40786 NN denotes software
T10190 40787-40795 VBN denotes supplied
T10191 40796-40800 IN denotes with
T10192 40801-40804 DT denotes the
T10193 40805-40815 NN denotes instrument
T10194 40815-40816 . denotes .
T10195 40816-40981 sentence denotes Serum biochemistry (ALP, Ca, PO4, Mg) was determined at the Rodent Diagnostics Lab (McGill University, Montréal, Quebec, Canada) using routine automated techniques.
T10196 40817-40822 NN denotes Serum
T10197 40823-40835 NN denotes biochemistry
T10199 40836-40837 -LRB- denotes (
T10201 40837-40840 NN denotes ALP
T10202 40840-40842 , denotes ,
T10203 40842-40844 NN denotes Ca
T10204 40844-40846 , denotes ,
T10205 40846-40849 NN denotes PO4
T10206 40849-40851 , denotes ,
T10200 40851-40853 NN denotes Mg
T10207 40853-40854 -RRB- denotes )
T10208 40855-40858 VBD denotes was
T10198 40859-40869 VBN denotes determined
T10209 40870-40872 IN denotes at
T10210 40873-40876 DT denotes the
T10212 40877-40883 NNP denotes Rodent
T10213 40884-40895 NNP denotes Diagnostics
T10211 40896-40899 NNP denotes Lab
T10214 40900-40901 -LRB- denotes (
T10216 40901-40907 NNP denotes McGill
T10215 40908-40918 NNP denotes University
T10217 40918-40920 , denotes ,
T10218 40920-40928 NNP denotes Montréal
T10219 40928-40930 , denotes ,
T10220 40930-40936 NNP denotes Quebec
T10221 40936-40938 , denotes ,
T10222 40938-40944 NNP denotes Canada
T10223 40944-40945 -RRB- denotes )
T10224 40946-40951 VBG denotes using
T10225 40952-40959 JJ denotes routine
T10227 40960-40969 VBN denotes automated
T10226 40970-40980 NNS denotes techniques
T10228 40980-40981 . denotes .
T10229 40981-41233 sentence denotes Commercial assays were used to determine serum levels of CTX (RatLaps ELISA, Nordic Bioscience), 17β estradiol (IBL Immuno-Biological, Hamburg, Germany), leptin (R & D Systems, Minneapolis, Minnesota, United States), and interleukin-6 (R & D Systems).
T10230 40982-40992 JJ denotes Commercial
T10231 40993-40999 NNS denotes assays
T10233 41000-41004 VBD denotes were
T10232 41005-41009 VBN denotes used
T10234 41010-41012 TO denotes to
T10235 41013-41022 VB denotes determine
T10236 41023-41028 NN denotes serum
T10237 41029-41035 NNS denotes levels
T10238 41036-41038 IN denotes of
T10239 41039-41042 NN denotes CTX
T10240 41043-41044 -LRB- denotes (
T10242 41044-41051 NNP denotes RatLaps
T10241 41052-41057 NNP denotes ELISA
T10243 41057-41059 , denotes ,
T10244 41059-41065 NNP denotes Nordic
T10245 41066-41076 NNP denotes Bioscience
T10246 41076-41077 -RRB- denotes )
T10247 41077-41079 , denotes ,
T10248 41079-41082 CD denotes 17β
T10249 41083-41092 NN denotes estradiol
T10250 41093-41094 -LRB- denotes (
T10252 41094-41097 NNP denotes IBL
T10251 41098-41115 NNP denotes Immuno-Biological
T10253 41115-41117 , denotes ,
T10254 41117-41124 NNP denotes Hamburg
T10255 41124-41126 , denotes ,
T10256 41126-41133 NNP denotes Germany
T10257 41133-41134 -RRB- denotes )
T10258 41134-41136 , denotes ,
T10259 41136-41142 NN denotes leptin
T10260 41143-41144 -LRB- denotes (
T10262 41144-41145 NNP denotes R
T10263 41146-41147 CC denotes &
T10264 41148-41149 NNP denotes D
T10261 41150-41157 NNP denotes Systems
T10265 41157-41159 , denotes ,
T10266 41159-41170 NNP denotes Minneapolis
T10267 41170-41172 , denotes ,
T10268 41172-41181 NNP denotes Minnesota
T10269 41181-41183 , denotes ,
T10270 41183-41189 NNP denotes United
T10271 41190-41196 NNP denotes States
T10272 41196-41197 -RRB- denotes )
T10273 41197-41199 , denotes ,
T10274 41199-41202 CC denotes and
T10275 41203-41214 NN denotes interleukin
T10276 41214-41215 HYPH denotes -
T10277 41215-41216 CD denotes 6
T10278 41217-41218 -LRB- denotes (
T10280 41218-41219 NNP denotes R
T10281 41220-41221 CC denotes &
T10282 41222-41223 NNP denotes D
T10279 41224-41231 NNP denotes Systems
T10283 41231-41232 -RRB- denotes )
T10284 41232-41233 . denotes .
T10388 41235-41237 FW denotes Ex
T10389 41238-41242 FW denotes vivo
T10390 41243-41253 NN denotes assessment
T10391 41254-41256 IN denotes of
T10392 41257-41267 NN denotes osteoblast
T10394 41268-41271 CC denotes and
T10395 41272-41282 NN denotes osteoclast
T10393 41283-41291 NN denotes activity
T10396 41291-41292 . denotes .
T10397 41292-41499 sentence denotes Bone marrow was flushed from the tibia and femora of juvenile 4-week-old Sam68+/+ and Sam68−/− mice to obtain stromal cells that were maintained in differentiation medium for 6 or 18 days as described [63].
T10398 41293-41297 NN denotes Bone
T10399 41298-41304 NN denotes marrow
T10401 41305-41308 VBD denotes was
T10400 41309-41316 VBN denotes flushed
T10402 41317-41321 IN denotes from
T10403 41322-41325 DT denotes the
T10404 41326-41331 NN denotes tibia
T10405 41332-41335 CC denotes and
T10406 41336-41342 NNS denotes femora
T10407 41343-41345 IN denotes of
T10408 41346-41354 JJ denotes juvenile
T10410 41355-41356 CD denotes 4
T10412 41356-41357 HYPH denotes -
T10411 41357-41361 NN denotes week
T10414 41361-41362 HYPH denotes -
T10413 41362-41365 JJ denotes old
T10415 41366-41371 NN denotes Sam68
T10416 41371-41372 SYM denotes +
T10417 41372-41373 HYPH denotes /
T10418 41373-41374 SYM denotes +
T10419 41375-41378 CC denotes and
T10420 41379-41384 NN denotes Sam68
T10421 41384-41385 SYM denotes −
T10422 41385-41386 HYPH denotes /
T10423 41386-41387 SYM denotes −
T10409 41388-41392 NNS denotes mice
T10424 41393-41395 TO denotes to
T10425 41396-41402 VB denotes obtain
T10426 41403-41410 JJ denotes stromal
T10427 41411-41416 NNS denotes cells
T10428 41417-41421 WDT denotes that
T10430 41422-41426 VBD denotes were
T10429 41427-41437 VBN denotes maintained
T10431 41438-41440 IN denotes in
T10432 41441-41456 NN denotes differentiation
T10433 41457-41463 NN denotes medium
T10434 41464-41467 IN denotes for
T10435 41468-41469 CD denotes 6
T10437 41470-41472 CC denotes or
T10438 41473-41475 CD denotes 18
T10436 41476-41480 NNS denotes days
T10439 41481-41483 IN denotes as
T10440 41484-41493 VBN denotes described
T10441 41494-41495 -LRB- denotes [
T10442 41495-41497 CD denotes 63
T10443 41497-41498 -RRB- denotes ]
T10444 41498-41499 . denotes .
T10445 41499-41602 sentence denotes Cultures were stained in situ for ALP activity and with von Kossa stain to detect mineralized nodules.
T10446 41500-41508 NNS denotes Cultures
T10448 41509-41513 VBD denotes were
T10447 41514-41521 VBN denotes stained
T10449 41522-41524 FW denotes in
T10450 41525-41529 FW denotes situ
T10451 41530-41533 IN denotes for
T10452 41534-41537 NN denotes ALP
T10453 41538-41546 NN denotes activity
T10454 41547-41550 CC denotes and
T10455 41551-41555 IN denotes with
T10456 41556-41559 NNP denotes von
T10457 41560-41565 NNP denotes Kossa
T10458 41566-41571 NN denotes stain
T10459 41572-41574 TO denotes to
T10460 41575-41581 VB denotes detect
T10461 41582-41593 VBN denotes mineralized
T10462 41594-41601 NNS denotes nodules
T10463 41601-41602 . denotes .
T10464 41602-41747 sentence denotes Total RNA was harvested from parallel cultures for RT-PCR analysis of osteoblast-related gene expression over time as described previously [63].
T10465 41603-41608 JJ denotes Total
T10466 41609-41612 NN denotes RNA
T10468 41613-41616 VBD denotes was
T10467 41617-41626 VBN denotes harvested
T10469 41627-41631 IN denotes from
T10470 41632-41640 JJ denotes parallel
T10471 41641-41649 NNS denotes cultures
T10472 41650-41653 IN denotes for
T10473 41654-41656 NN denotes RT
T10475 41656-41657 HYPH denotes -
T10474 41657-41660 NN denotes PCR
T10476 41661-41669 NN denotes analysis
T10477 41670-41672 IN denotes of
T10478 41673-41683 NN denotes osteoblast
T10480 41683-41684 HYPH denotes -
T10479 41684-41691 VBN denotes related
T10482 41692-41696 NN denotes gene
T10481 41697-41707 NN denotes expression
T10483 41708-41712 IN denotes over
T10484 41713-41717 NN denotes time
T10485 41718-41720 IN denotes as
T10486 41721-41730 VBN denotes described
T10487 41731-41741 RB denotes previously
T10488 41742-41743 -LRB- denotes [
T10489 41743-41745 CD denotes 63
T10490 41745-41746 -RRB- denotes ]
T10491 41746-41747 . denotes .
T10492 41747-41893 sentence denotes Osteoclasts were isolated from the crushed long bones of juvenile Sam68+/+ and Sam68−/− mice and used for quantitative studies as described [64].
T10493 41748-41759 NNS denotes Osteoclasts
T10495 41760-41764 VBD denotes were
T10494 41765-41773 VBN denotes isolated
T10496 41774-41778 IN denotes from
T10497 41779-41782 DT denotes the
T10499 41783-41790 VBN denotes crushed
T10500 41791-41795 JJ denotes long
T10498 41796-41801 NNS denotes bones
T10501 41802-41804 IN denotes of
T10502 41805-41813 JJ denotes juvenile
T10504 41814-41819 NN denotes Sam68
T10505 41819-41820 SYM denotes +
T10506 41820-41821 HYPH denotes /
T10507 41821-41822 SYM denotes +
T10508 41823-41826 CC denotes and
T10509 41827-41832 NN denotes Sam68
T10510 41832-41833 SYM denotes −
T10511 41833-41834 HYPH denotes /
T10512 41834-41835 SYM denotes −
T10503 41836-41840 NNS denotes mice
T10513 41841-41844 CC denotes and
T10514 41845-41849 VBN denotes used
T10515 41850-41853 IN denotes for
T10516 41854-41866 JJ denotes quantitative
T10517 41867-41874 NNS denotes studies
T10518 41875-41877 IN denotes as
T10519 41878-41887 VBN denotes described
T10520 41888-41889 -LRB- denotes [
T10521 41889-41891 CD denotes 64
T10522 41891-41892 -RRB- denotes ]
T10523 41892-41893 . denotes .
T10524 41893-42046 sentence denotes Briefly, cells were plated on glass coverslips or on dentin slices in 24-well cluster plates for assessment of cell number and pit number, respectively.
T10525 41894-41901 RB denotes Briefly
T10527 41901-41903 , denotes ,
T10528 41903-41908 NNS denotes cells
T10529 41909-41913 VBD denotes were
T10526 41914-41920 VBN denotes plated
T10530 41921-41923 IN denotes on
T10531 41924-41929 NN denotes glass
T10532 41930-41940 NNS denotes coverslips
T10533 41941-41943 CC denotes or
T10534 41944-41946 IN denotes on
T10535 41947-41953 NN denotes dentin
T10536 41954-41960 NNS denotes slices
T10537 41961-41963 IN denotes in
T10538 41964-41966 CD denotes 24
T10540 41966-41967 HYPH denotes -
T10539 41967-41971 NN denotes well
T10542 41972-41979 NN denotes cluster
T10541 41980-41986 NNS denotes plates
T10543 41987-41990 IN denotes for
T10544 41991-42001 NN denotes assessment
T10545 42002-42004 IN denotes of
T10546 42005-42009 NN denotes cell
T10547 42010-42016 NN denotes number
T10548 42017-42020 CC denotes and
T10549 42021-42024 NN denotes pit
T10550 42025-42031 NN denotes number
T10551 42031-42033 , denotes ,
T10552 42033-42045 RB denotes respectively
T10553 42045-42046 . denotes .
T10554 42046-42136 sentence denotes Cover slips were immersed in 4% paraformaldehyde and stained for TRAP activity after 2 h.
T10555 42047-42052 NN denotes Cover
T10556 42053-42058 NNS denotes slips
T10558 42059-42063 VBD denotes were
T10557 42064-42072 VBN denotes immersed
T10559 42073-42075 IN denotes in
T10560 42076-42077 CD denotes 4
T10561 42077-42078 NN denotes %
T10562 42079-42095 NN denotes paraformaldehyde
T10563 42096-42099 CC denotes and
T10564 42100-42107 VBN denotes stained
T10565 42108-42111 IN denotes for
T10566 42112-42116 NN denotes TRAP
T10567 42117-42125 NN denotes activity
T10568 42126-42131 IN denotes after
T10569 42132-42133 CD denotes 2
T10570 42134-42135 NN denotes h
T10571 42135-42136 . denotes .
T10572 42136-42359 sentence denotes Dentin slices were left for 28 h before removing the cells with 1 M ammonium hydroxide and air drying before coating with sputter Au-Pd and examination with scanning electron microscopy (McGill Electron Microscopy Centre).
T10573 42137-42143 NN denotes Dentin
T10574 42144-42150 NNS denotes slices
T10576 42151-42155 VBD denotes were
T10575 42156-42160 VBN denotes left
T10577 42161-42164 IN denotes for
T10578 42165-42167 CD denotes 28
T10579 42168-42169 NN denotes h
T10580 42170-42176 IN denotes before
T10581 42177-42185 VBG denotes removing
T10582 42186-42189 DT denotes the
T10583 42190-42195 NNS denotes cells
T10584 42196-42200 IN denotes with
T10585 42201-42202 CD denotes 1
T10586 42203-42204 NN denotes M
T10588 42205-42213 NN denotes ammonium
T10587 42214-42223 NN denotes hydroxide
T10589 42224-42227 CC denotes and
T10590 42228-42231 NN denotes air
T10591 42232-42238 VBG denotes drying
T10592 42239-42245 IN denotes before
T10593 42246-42253 VBG denotes coating
T10594 42254-42258 IN denotes with
T10595 42259-42266 NN denotes sputter
T10597 42267-42269 NN denotes Au
T10598 42269-42270 HYPH denotes -
T10596 42270-42272 NN denotes Pd
T10599 42273-42276 CC denotes and
T10600 42277-42288 NN denotes examination
T10601 42289-42293 IN denotes with
T10602 42294-42302 VBG denotes scanning
T10604 42303-42311 NN denotes electron
T10603 42312-42322 NN denotes microscopy
T10605 42323-42324 -LRB- denotes (
T10607 42324-42330 NNP denotes McGill
T10608 42331-42339 NNP denotes Electron
T10609 42340-42350 NNP denotes Microscopy
T10606 42351-42357 NNP denotes Centre
T10610 42357-42358 -RRB- denotes )
T10611 42358-42359 . denotes .
T10612 42359-42462 sentence denotes Light microscope images were captured using a Leica DMR microscope equipped with a Retiga 1300 camera.
T10613 42360-42365 NN denotes Light
T10614 42366-42376 NN denotes microscope
T10615 42377-42383 NNS denotes images
T10617 42384-42388 VBD denotes were
T10616 42389-42397 VBN denotes captured
T10618 42398-42403 VBG denotes using
T10619 42404-42405 DT denotes a
T10621 42406-42411 NNP denotes Leica
T10622 42412-42415 NNP denotes DMR
T10620 42416-42426 NN denotes microscope
T10623 42427-42435 VBN denotes equipped
T10624 42436-42440 IN denotes with
T10625 42441-42442 DT denotes a
T10627 42443-42449 NNP denotes Retiga
T10628 42450-42454 CD denotes 1300
T10626 42455-42461 NN denotes camera
T10629 42461-42462 . denotes .
T10630 42462-42602 sentence denotes Quantitative analyses were performed using Adobe Photoshop and the data presented as the mean ± SD of two or three independent experiments.
T10631 42463-42475 JJ denotes Quantitative
T10632 42476-42484 NNS denotes analyses
T10634 42485-42489 VBD denotes were
T10633 42490-42499 VBN denotes performed
T10635 42500-42505 VBG denotes using
T10636 42506-42511 NNP denotes Adobe
T10637 42512-42521 NNP denotes Photoshop
T10638 42522-42525 CC denotes and
T10639 42526-42529 DT denotes the
T10640 42530-42534 NNS denotes data
T10641 42535-42544 VBN denotes presented
T10642 42545-42547 IN denotes as
T10643 42548-42551 DT denotes the
T10645 42552-42556 NN denotes mean
T10646 42557-42558 SYM denotes ±
T10644 42559-42561 NN denotes SD
T10647 42562-42564 IN denotes of
T10648 42565-42568 CD denotes two
T10650 42569-42571 CC denotes or
T10651 42572-42577 CD denotes three
T10652 42578-42589 JJ denotes independent
T10649 42590-42601 NNS denotes experiments
T10653 42601-42602 . denotes .
T10654 42602-42664 sentence denotes Statistical comparisons were made using the Student's t test.
T10655 42603-42614 JJ denotes Statistical
T10656 42615-42626 NNS denotes comparisons
T10658 42627-42631 VBD denotes were
T10657 42632-42636 VBN denotes made
T10659 42637-42642 VBG denotes using
T10660 42643-42646 DT denotes the
T10661 42647-42654 NNP denotes Student
T10663 42654-42656 POS denotes 's
T10664 42657-42658 NN denotes t
T10662 42659-42663 NN denotes test
T10665 42663-42664 . denotes .
T10693 42666-42677 NN denotes Preparation
T10694 42678-42680 IN denotes of
T10695 42681-42686 NN denotes mouse
T10697 42687-42696 JJ denotes embryonic
T10696 42697-42707 NN denotes fibroblast
T10698 42708-42711 CC denotes and
T10699 42712-42721 NN denotes induction
T10700 42722-42724 IN denotes of
T10701 42725-42734 NN denotes adipocyte
T10702 42735-42750 NN denotes differentiation
T10703 42750-42751 . denotes .
T10704 42751-42797 sentence denotes MEFs were prepared from 14.5-day-old embryos.
T10705 42752-42756 NNS denotes MEFs
T10707 42757-42761 VBD denotes were
T10706 42762-42770 VBN denotes prepared
T10708 42771-42775 IN denotes from
T10709 42776-42780 CD denotes 14.5
T10711 42780-42781 HYPH denotes -
T10710 42781-42784 NN denotes day
T10713 42784-42785 HYPH denotes -
T10712 42785-42788 JJ denotes old
T10714 42789-42796 NNS denotes embryos
T10715 42796-42797 . denotes .
T10716 42797-42972 sentence denotes Only early-passage MEFs were used for the experimental studies, and induction of adipocyte differentiation was carried out according to the methods previously described [30].
T10717 42798-42802 RB denotes Only
T10719 42803-42808 JJ denotes early
T10721 42808-42809 HYPH denotes -
T10720 42809-42816 NN denotes passage
T10718 42817-42821 NNS denotes MEFs
T10723 42822-42826 VBD denotes were
T10722 42827-42831 VBN denotes used
T10724 42832-42835 IN denotes for
T10725 42836-42839 DT denotes the
T10727 42840-42852 JJ denotes experimental
T10726 42853-42860 NNS denotes studies
T10728 42860-42862 , denotes ,
T10729 42862-42865 CC denotes and
T10730 42866-42875 NN denotes induction
T10732 42876-42878 IN denotes of
T10733 42879-42888 NN denotes adipocyte
T10734 42889-42904 NN denotes differentiation
T10735 42905-42908 VBD denotes was
T10731 42909-42916 VBN denotes carried
T10736 42917-42920 RP denotes out
T10737 42921-42930 VBG denotes according
T10738 42931-42933 IN denotes to
T10739 42934-42937 DT denotes the
T10740 42938-42945 NNS denotes methods
T10741 42946-42956 RB denotes previously
T10742 42957-42966 VBN denotes described
T10743 42967-42968 -LRB- denotes [
T10744 42968-42970 CD denotes 30
T10745 42970-42971 -RRB- denotes ]
T10746 42971-42972 . denotes .
T10747 42972-43034 sentence denotes Cultured cells were stained with Oil Red O as described [29].
T10748 42973-42981 VBN denotes Cultured
T10749 42982-42987 NNS denotes cells
T10751 42988-42992 VBD denotes were
T10750 42993-43000 VBN denotes stained
T10752 43001-43005 IN denotes with
T10753 43006-43009 NN denotes Oil
T10755 43010-43013 NN denotes Red
T10754 43014-43015 NN denotes O
T10756 43016-43018 IN denotes as
T10757 43019-43028 VBN denotes described
T10758 43029-43030 -LRB- denotes [
T10759 43030-43032 CD denotes 29
T10760 43032-43033 -RRB- denotes ]
T10761 43033-43034 . denotes .
T10818 43036-43039 NN denotes RNA
T10819 43040-43051 NN denotes preparation
T10820 43052-43055 CC denotes and
T10821 43056-43058 NN denotes RT
T10823 43058-43059 HYPH denotes -
T10822 43059-43062 NN denotes PCR
T10824 43063-43065 TO denotes to
T10825 43066-43072 VB denotes assess
T10826 43073-43085 NN denotes adipogenesis
T10827 43086-43088 IN denotes in
T10828 43089-43094 NN denotes Sam68
T10830 43094-43095 SYM denotes −
T10831 43095-43096 HYPH denotes /
T10832 43096-43097 SYM denotes −
T10829 43098-43102 NNS denotes MEFs
T10833 43102-43103 . denotes .
T10834 43103-43245 sentence denotes Total cellular RNA was prepared by TRIzol reagent according to the manufacturer's protocol (Invitrogen, Carlsbad, California, United States).
T10835 43104-43109 JJ denotes Total
T10837 43110-43118 JJ denotes cellular
T10836 43119-43122 NN denotes RNA
T10839 43123-43126 VBD denotes was
T10838 43127-43135 VBN denotes prepared
T10840 43136-43138 IN denotes by
T10841 43139-43145 NN denotes TRIzol
T10842 43146-43153 NN denotes reagent
T10843 43154-43163 VBG denotes according
T10844 43164-43166 IN denotes to
T10845 43167-43170 DT denotes the
T10846 43171-43183 NN denotes manufacturer
T10848 43183-43185 POS denotes 's
T10847 43186-43194 NN denotes protocol
T10849 43195-43196 -LRB- denotes (
T10850 43196-43206 NNP denotes Invitrogen
T10851 43206-43208 , denotes ,
T10852 43208-43216 NNP denotes Carlsbad
T10853 43216-43218 , denotes ,
T10854 43218-43228 NNP denotes California
T10855 43228-43230 , denotes ,
T10856 43230-43236 NNP denotes United
T10857 43237-43243 NNP denotes States
T10858 43243-43244 -RRB- denotes )
T10859 43244-43245 . denotes .
T10860 43245-43327 sentence denotes Total RNA (1 μg) was reverse-transcribed, and cDNA samples were subjected to PCR.
T10861 43246-43251 JJ denotes Total
T10862 43252-43255 NN denotes RNA
T10864 43256-43257 -LRB- denotes (
T10866 43257-43258 CD denotes 1
T10865 43259-43261 NN denotes μg
T10867 43261-43262 -RRB- denotes )
T10868 43263-43266 VBD denotes was
T10869 43267-43274 RB denotes reverse
T10870 43274-43275 HYPH denotes -
T10863 43275-43286 VBN denotes transcribed
T10871 43286-43288 , denotes ,
T10872 43288-43291 CC denotes and
T10873 43292-43296 NN denotes cDNA
T10874 43297-43304 NNS denotes samples
T10876 43305-43309 VBD denotes were
T10875 43310-43319 VBN denotes subjected
T10877 43320-43322 IN denotes to
T10878 43323-43326 NN denotes PCR
T10879 43326-43327 . denotes .
T10880 43327-43388 sentence denotes RT-PCR was normalized by the transcriptional level of GAPDH.
T10881 43328-43330 NN denotes RT
T10883 43330-43331 HYPH denotes -
T10882 43331-43334 NN denotes PCR
T10885 43335-43338 VBD denotes was
T10884 43339-43349 VBN denotes normalized
T10886 43350-43352 IN denotes by
T10887 43353-43356 DT denotes the
T10889 43357-43372 JJ denotes transcriptional
T10888 43373-43378 NN denotes level
T10890 43379-43381 IN denotes of
T10891 43382-43387 NN denotes GAPDH
T10892 43387-43388 . denotes .
T10893 43388-43789 sentence denotes The following 5′ and 3′ primers were used to evaluate adipogenic differentiation: KLF5: 5'-AGA CAA GCT GAG ATG CTG C-3′, 5′-GGC AAA CCT CCA GTC GC-3′; PPARγ: 5′-GTG CGA TCA AAG TAG AAC CTG C-3′, 5′-CCT ATC ATA AAT AAG CTT CAA TCG-3′; β-Actin: 5′-TAG GCG GAC TGT TAC TGA GC-3′, 5′-AGC CTT CAT ACA TCA AGT TGG-3′; and Sam68: 5′- GTG GAG ACC CCA AAT ATG CCC A-3′ and 5′-AAA CTG CTC CTG ACA GAT ATC A-3′.
T10894 43389-43392 DT denotes The
T10896 43393-43402 VBG denotes following
T10897 43403-43404 CD denotes 5
T10898 43404-43405 SYM denotes ′
T10899 43406-43409 CC denotes and
T10900 43410-43411 CD denotes 3
T10901 43411-43412 SYM denotes ′
T10895 43413-43420 NNS denotes primers
T10903 43421-43425 VBD denotes were
T10902 43426-43430 VBN denotes used
T10904 43431-43433 TO denotes to
T10905 43434-43442 VB denotes evaluate
T10906 43443-43453 JJ denotes adipogenic
T10907 43454-43469 NN denotes differentiation
T10908 43469-43471 : denotes :
T10909 43471-43475 NN denotes KLF5
T10910 43475-43477 : denotes :
T10911 43477-43478 CD denotes 5
T10912 43478-43479 SYM denotes '
T10913 43479-43480 HYPH denotes -
T10914 43480-43483 NN denotes AGA
T10916 43484-43487 NN denotes CAA
T10917 43488-43491 NN denotes GCT
T10918 43492-43495 NN denotes GAG
T10919 43496-43499 NN denotes ATG
T10920 43500-43503 NN denotes CTG
T10915 43504-43505 NN denotes C
T10921 43505-43506 HYPH denotes -
T10922 43506-43507 CD denotes 3
T10923 43507-43508 SYM denotes ′
T10924 43508-43510 , denotes ,
T10925 43510-43511 CD denotes 5
T10926 43511-43512 SYM denotes ′
T10927 43512-43513 HYPH denotes -
T10928 43513-43516 NN denotes GGC
T10930 43517-43520 NN denotes AAA
T10931 43521-43524 NN denotes CCT
T10932 43525-43528 NN denotes CCA
T10933 43529-43532 NN denotes GTC
T10929 43533-43535 NN denotes GC
T10934 43535-43536 HYPH denotes -
T10935 43536-43537 CD denotes 3
T10936 43537-43538 SYM denotes ′
T10937 43538-43539 : denotes ;
T10938 43540-43545 NN denotes PPARγ
T10939 43545-43547 : denotes :
T10940 43547-43548 CD denotes 5
T10941 43548-43549 SYM denotes ′
T10942 43549-43550 HYPH denotes -
T10943 43550-43553 NN denotes GTG
T10945 43554-43557 NN denotes CGA
T10946 43558-43561 NN denotes TCA
T10947 43562-43565 NN denotes AAG
T10948 43566-43569 NN denotes TAG
T10949 43570-43573 NN denotes AAC
T10950 43574-43577 NN denotes CTG
T10944 43578-43579 NN denotes C
T10951 43579-43580 HYPH denotes -
T10952 43580-43581 CD denotes 3
T10953 43581-43582 SYM denotes ′
T10954 43582-43584 , denotes ,
T10955 43584-43585 CD denotes 5
T10956 43585-43586 SYM denotes ′
T10957 43586-43587 HYPH denotes -
T10958 43587-43590 NN denotes CCT
T10960 43591-43594 NN denotes ATC
T10961 43595-43598 NN denotes ATA
T10962 43599-43602 NN denotes AAT
T10963 43603-43606 NN denotes AAG
T10964 43607-43610 NN denotes CTT
T10965 43611-43614 NN denotes CAA
T10959 43615-43618 NN denotes TCG
T10966 43618-43619 HYPH denotes -
T10967 43619-43620 CD denotes 3
T10968 43620-43621 SYM denotes ′
T10969 43621-43622 : denotes ;
T10970 43623-43624 NN denotes β
T10972 43624-43625 HYPH denotes -
T10971 43625-43630 NN denotes Actin
T10973 43630-43632 : denotes :
T10974 43632-43633 CD denotes 5
T10975 43633-43634 SYM denotes ′
T10976 43634-43635 HYPH denotes -
T10977 43635-43638 NN denotes TAG
T10979 43639-43642 NN denotes GCG
T10980 43643-43646 NN denotes GAC
T10981 43647-43650 NN denotes TGT
T10982 43651-43654 NN denotes TAC
T10983 43655-43658 NN denotes TGA
T10978 43659-43661 NN denotes GC
T10984 43661-43662 HYPH denotes -
T10985 43662-43663 CD denotes 3
T10986 43663-43664 SYM denotes ′
T10987 43664-43666 , denotes ,
T10988 43666-43667 CD denotes 5
T10989 43667-43668 SYM denotes ′
T10990 43668-43669 HYPH denotes -
T10991 43669-43672 NN denotes AGC
T10993 43673-43676 NN denotes CTT
T10994 43677-43680 NN denotes CAT
T10995 43681-43684 NN denotes ACA
T10996 43685-43688 NN denotes TCA
T10997 43689-43692 NN denotes AGT
T10992 43693-43696 NN denotes TGG
T10998 43696-43697 HYPH denotes -
T10999 43697-43698 CD denotes 3
T11000 43698-43699 SYM denotes ′
T11001 43699-43700 : denotes ;
T11002 43701-43704 CC denotes and
T11003 43705-43710 NN denotes Sam68
T11004 43710-43712 : denotes :
T11005 43712-43713 CD denotes 5
T11006 43713-43714 SYM denotes ′
T11007 43714-43715 HYPH denotes -
T11008 43716-43719 NN denotes GTG
T11010 43720-43723 NN denotes GAG
T11011 43724-43727 NN denotes ACC
T11012 43728-43731 NN denotes CCA
T11013 43732-43735 NN denotes AAT
T11014 43736-43739 NN denotes ATG
T11015 43740-43743 NN denotes CCC
T11009 43744-43745 NN denotes A
T11016 43745-43746 HYPH denotes -
T11017 43746-43747 CD denotes 3
T11018 43747-43748 SYM denotes ′
T11019 43749-43752 CC denotes and
T11020 43753-43754 CD denotes 5
T11021 43754-43755 SYM denotes ′
T11022 43755-43756 HYPH denotes -
T11023 43756-43759 NN denotes AAA
T11025 43760-43763 NN denotes CTG
T11026 43764-43767 NN denotes CTC
T11027 43768-43771 NN denotes CTG
T11028 43772-43775 NN denotes ACA
T11029 43776-43779 NN denotes GAT
T11030 43780-43783 NN denotes ATC
T11024 43784-43785 NN denotes A
T11031 43785-43786 HYPH denotes -
T11032 43786-43787 CD denotes 3
T11033 43787-43788 SYM denotes ′
T11034 43788-43789 . denotes .
T11076 43791-43796 NN denotes Sam68
T11078 43797-43801 RB denotes down
T11079 43801-43802 HYPH denotes -
T11077 43802-43812 NN denotes regulation
T11080 43813-43818 VBG denotes using
T11081 43819-43826 NN denotes Sam68sh
T11082 43826-43827 . denotes .
T11083 43827-43912 sentence denotes To generate an shRNA, 64-base duplex DNA nucleotides were purchased from Invitrogen.
T11084 43828-43830 TO denotes To
T11085 43831-43839 VB denotes generate
T11087 43840-43842 DT denotes an
T11088 43843-43848 NN denotes shRNA
T11089 43848-43850 , denotes ,
T11090 43850-43852 CD denotes 64
T11092 43852-43853 HYPH denotes -
T11091 43853-43857 NN denotes base
T11094 43858-43864 NN denotes duplex
T11095 43865-43868 NN denotes DNA
T11093 43869-43880 NNS denotes nucleotides
T11096 43881-43885 VBD denotes were
T11086 43886-43895 VBN denotes purchased
T11097 43896-43900 IN denotes from
T11098 43901-43911 NNP denotes Invitrogen
T11099 43911-43912 . denotes .
T11100 43912-44048 sentence denotes This fragment comprised 19 base residues specific to mouse Sam68 (GATCCCC AAGATGACGAGGAGAATTATTCAAGAGA TAATTCTCCTCGTCATCTT TTTTTGGAAA).
T11101 43913-43917 DT denotes This
T11102 43918-43926 NN denotes fragment
T11103 43927-43936 VBD denotes comprised
T11104 43937-43939 CD denotes 19
T11106 43940-43944 NN denotes base
T11105 43945-43953 NNS denotes residues
T11107 43954-43962 JJ denotes specific
T11108 43963-43965 IN denotes to
T11109 43966-43971 NN denotes mouse
T11110 43972-43977 NN denotes Sam68
T11111 43978-43979 -LRB- denotes (
T11113 43979-43986 NN denotes GATCCCC
T11114 43987-44015 NN denotes AAGATGACGAGGAGAATTATTCAAGAGA
T11115 44016-44035 NN denotes TAATTCTCCTCGTCATCTT
T11112 44036-44046 NN denotes TTTTTGGAAA
T11116 44046-44047 -RRB- denotes )
T11117 44047-44048 . denotes .
T11118 44048-44142 sentence denotes This fragment was cloned into pSUPER retro (OligoEngine, Seattle, Washington, United States).
T11119 44049-44053 DT denotes This
T11120 44054-44062 NN denotes fragment
T11122 44063-44066 VBD denotes was
T11121 44067-44073 VBN denotes cloned
T11123 44074-44078 IN denotes into
T11124 44079-44085 NNP denotes pSUPER
T11125 44086-44091 NNP denotes retro
T11126 44092-44093 -LRB- denotes (
T11127 44093-44104 NNP denotes OligoEngine
T11128 44104-44106 , denotes ,
T11129 44106-44113 NNP denotes Seattle
T11130 44113-44115 , denotes ,
T11131 44115-44125 NNP denotes Washington
T11132 44125-44127 , denotes ,
T11133 44127-44133 NNP denotes United
T11134 44134-44140 NNP denotes States
T11135 44140-44141 -RRB- denotes )
T11136 44141-44142 . denotes .
T11137 44142-44251 sentence denotes Transfections were performed using LipofectAMINE Plus (Invitrogen) with cloned DNA fragment or empty vector.
T11138 44143-44156 NNS denotes Transfections
T11140 44157-44161 VBD denotes were
T11139 44162-44171 VBN denotes performed
T11141 44172-44177 VBG denotes using
T11142 44178-44191 NNP denotes LipofectAMINE
T11143 44192-44196 NNP denotes Plus
T11144 44197-44198 -LRB- denotes (
T11145 44198-44208 NNP denotes Invitrogen
T11146 44208-44209 -RRB- denotes )
T11147 44210-44214 IN denotes with
T11148 44215-44221 VBN denotes cloned
T11150 44222-44225 NN denotes DNA
T11149 44226-44234 NN denotes fragment
T11151 44235-44237 CC denotes or
T11152 44238-44243 JJ denotes empty
T11153 44244-44250 NN denotes vector
T11154 44250-44251 . denotes .
T11155 44251-44406 sentence denotes At 48 h after transfection, populations were selected for 2.5 μg/ml puromycin and the knockdown observed by immunoblotting with anti-Sam68 (AD1) antibody.
T11156 44252-44254 IN denotes At
T11158 44255-44257 CD denotes 48
T11159 44258-44259 NN denotes h
T11160 44260-44265 IN denotes after
T11161 44266-44278 NN denotes transfection
T11162 44278-44280 , denotes ,
T11163 44280-44291 NNS denotes populations
T11164 44292-44296 VBD denotes were
T11157 44297-44305 VBN denotes selected
T11165 44306-44309 IN denotes for
T11166 44310-44313 CD denotes 2.5
T11167 44314-44316 NN denotes μg
T11169 44316-44317 SYM denotes /
T11170 44317-44319 NN denotes ml
T11168 44320-44329 NN denotes puromycin
T11171 44330-44333 CC denotes and
T11172 44334-44337 DT denotes the
T11173 44338-44347 NN denotes knockdown
T11174 44348-44356 VBN denotes observed
T11175 44357-44359 IN denotes by
T11176 44360-44374 VBG denotes immunoblotting
T11177 44375-44379 IN denotes with
T11178 44380-44390 JJ denotes anti-Sam68
T11180 44391-44392 -LRB- denotes (
T11181 44392-44395 NN denotes AD1
T11182 44395-44396 -RRB- denotes )
T11179 44397-44405 NN denotes antibody
T11183 44405-44406 . denotes .
T11232 44408-44418 JJ denotes Osteogenic
T11234 44419-44434 NN denotes differentiation
T11233 44435-44443 NN denotes analysis
T11235 44444-44446 IN denotes of
T11236 44447-44454 NN denotes C3HT101
T11238 44454-44455 HYPH denotes /
T11239 44455-44456 CD denotes 2
T11237 44457-44462 NNS denotes cells
T11240 44462-44463 . denotes .
T11241 44463-44592 sentence denotes For osteogenic differentiation, C3HT101/2 (ATCC) cells were incubated in 48-well tissue culture plates at 1.25 × 104 cells/cm2 .
T11242 44464-44467 IN denotes For
T11244 44468-44478 JJ denotes osteogenic
T11245 44479-44494 NN denotes differentiation
T11246 44494-44496 , denotes ,
T11247 44496-44503 NN denotes C3HT101
T11249 44503-44504 HYPH denotes /
T11250 44504-44505 CD denotes 2
T11251 44506-44507 -LRB- denotes (
T11252 44507-44511 NN denotes ATCC
T11253 44511-44512 -RRB- denotes )
T11248 44513-44518 NNS denotes cells
T11254 44519-44523 VBD denotes were
T11243 44524-44533 VBN denotes incubated
T11255 44534-44536 IN denotes in
T11256 44537-44539 CD denotes 48
T11258 44539-44540 HYPH denotes -
T11257 44540-44544 NN denotes well
T11260 44545-44551 NN denotes tissue
T11261 44552-44559 NN denotes culture
T11259 44560-44566 NNS denotes plates
T11262 44567-44569 IN denotes at
T11263 44570-44574 CD denotes 1.25
T11265 44575-44576 SYM denotes ×
T11264 44577-44580 CD denotes 104
T11266 44581-44586 NNS denotes cells
T11267 44586-44587 SYM denotes /
T11268 44587-44590 NN denotes cm2
T11269 44591-44592 . denotes .
T11270 44592-44733 sentence denotes After 24-h incubation, the culture media were changed to fresh media containing 300 ng/ml BMP-2 (Sigma, St. Louis, Missouri, United States).
T11271 44593-44598 IN denotes After
T11273 44599-44601 CD denotes 24
T11275 44601-44602 HYPH denotes -
T11274 44602-44603 NN denotes h
T11276 44604-44614 NN denotes incubation
T11277 44614-44616 , denotes ,
T11278 44616-44619 DT denotes the
T11280 44620-44627 NN denotes culture
T11279 44628-44633 NNS denotes media
T11281 44634-44638 VBD denotes were
T11272 44639-44646 VBN denotes changed
T11282 44647-44649 IN denotes to
T11283 44650-44655 JJ denotes fresh
T11284 44656-44661 NNS denotes media
T11285 44662-44672 VBG denotes containing
T11286 44673-44676 CD denotes 300
T11287 44677-44679 NN denotes ng
T11289 44679-44680 SYM denotes /
T11290 44680-44682 NN denotes ml
T11288 44683-44686 NN denotes BMP
T11291 44686-44687 HYPH denotes -
T11292 44687-44688 CD denotes 2
T11293 44689-44690 -LRB- denotes (
T11294 44690-44695 NNP denotes Sigma
T11295 44695-44697 , denotes ,
T11296 44697-44700 NNP denotes St.
T11297 44701-44706 NNP denotes Louis
T11298 44706-44708 , denotes ,
T11299 44708-44716 NNP denotes Missouri
T11300 44716-44718 , denotes ,
T11301 44718-44724 NNP denotes United
T11302 44725-44731 NNP denotes States
T11303 44731-44732 -RRB- denotes )
T11304 44732-44733 . denotes .
T11305 44733-44904 sentence denotes Total cellular RNA was prepared as described above, and osteocalcin, β-actin, and GAPDH gene expression at indicated times after BMP-2 treatment was quantified by RT-PCR.
T11306 44734-44739 JJ denotes Total
T11308 44740-44748 JJ denotes cellular
T11307 44749-44752 NN denotes RNA
T11310 44753-44756 VBD denotes was
T11309 44757-44765 VBN denotes prepared
T11311 44766-44768 IN denotes as
T11312 44769-44778 VBN denotes described
T11313 44779-44784 RB denotes above
T11314 44784-44786 , denotes ,
T11315 44786-44789 CC denotes and
T11316 44790-44801 NN denotes osteocalcin
T11318 44801-44803 , denotes ,
T11319 44803-44804 NN denotes β
T11321 44804-44805 HYPH denotes -
T11320 44805-44810 NN denotes actin
T11322 44810-44812 , denotes ,
T11323 44812-44815 CC denotes and
T11324 44816-44821 NN denotes GAPDH
T11325 44822-44826 NN denotes gene
T11317 44827-44837 NN denotes expression
T11327 44838-44840 IN denotes at
T11328 44841-44850 VBN denotes indicated
T11329 44851-44856 NNS denotes times
T11330 44857-44862 IN denotes after
T11331 44863-44866 NN denotes BMP
T11333 44866-44867 HYPH denotes -
T11334 44867-44868 CD denotes 2
T11332 44869-44878 NN denotes treatment
T11335 44879-44882 VBD denotes was
T11326 44883-44893 VBN denotes quantified
T11336 44894-44896 IN denotes by
T11337 44897-44899 NN denotes RT
T11339 44899-44900 HYPH denotes -
T11338 44900-44903 NN denotes PCR
T11340 44903-44904 . denotes .
R1000 T1712 T1713 det a,substrate
R1001 T1765 T1760 nsubj we,report
R1002 T1766 T1767 det the,generation
R1003 T1767 T1760 dobj generation,report
R1004 T1768 T1767 prep of,generation
R1005 T1713 T1710 attr substrate,is
R1006 T1769 T1770 nmod Sam68,mice
R1007 T1770 T1768 pobj mice,of
R1008 T1714 T1713 amod known,substrate
R1009 T1771 T1769 punct −,Sam68
R1010 T1772 T1769 punct /,Sam68
R1011 T1773 T1769 punct −,Sam68
R1012 T1774 T1767 cc and,generation
R1013 T1775 T1767 conj analysis,generation
R1014 T1776 T1775 prep of,analysis
R1015 T1777 T1778 poss their,phenotype
R1016 T1715 T1713 prep of,substrate
R1017 T1778 T1776 pobj phenotype,of
R1018 T1779 T1778 amod skeletal,phenotype
R1019 T1780 T1760 punct .,report
R1020 T1716 T1717 compound Src,kinases
R1021 T1782 T1783 poss Our,data
R1022 T1717 T1715 pobj kinases,of
R1023 T1783 T1784 nsubj data,indicate
R1024 T1785 T1786 mark that,confers
R1025 T1718 T1719 punct [,39
R1026 T1786 T1784 ccomp confers,indicate
R1027 T1787 T1788 det the,absence
R1028 T1719 T1717 parataxis 39,kinases
R1029 T1788 T1786 nsubj absence,confers
R1030 T1789 T1788 prep of,absence
R1031 T1790 T1789 pobj Sam68,of
R1032 T1720 T1721 punct –,42
R1033 T1791 T1786 dobj resistance,confers
R1034 T1792 T1791 prep to,resistance
R1035 T1793 T1794 npadvmod age,related
R1036 T1826 T1827 nmod bone,marrow
R1037 T1794 T1796 amod related,loss
R1038 T1795 T1794 punct -,related
R1039 T1796 T1792 pobj loss,to
R1040 T1797 T1796 compound bone,loss
R1041 T1798 T1786 prep in,confers
R1042 T1799 T1798 pobj mice,in
R1043 T1827 T1828 nmod marrow,cells
R1044 T1800 T1801 amod such,have
R1045 T1801 T1786 advcl have,confers
R1046 T1802 T1801 mark that,have
R1047 T1828 T1825 pobj cells,of
R1048 T1803 T1804 amod old,Sam68
R1049 T1804 T1801 nsubj Sam68,have
R1050 T1805 T1806 det a,mass
R1051 T1829 T1828 amod stromal,cells
R1052 T1806 T1801 dobj mass,have
R1053 T1807 T1806 amod higher,mass
R1054 T1808 T1806 compound bone,mass
R1055 T1830 T1818 prep by,provide
R1056 T1809 T1806 prep than,mass
R1057 T1810 T1811 poss their,littermates
R1058 T1811 T1809 pobj littermates,than
R1059 T1831 T1830 pcomp showing,by
R1060 T1812 T1813 amod wild,type
R1061 T1813 T1811 compound type,littermates
R1062 T1814 T1813 punct -,type
R1063 T1815 T1784 punct .,indicate
R1064 T1817 T1818 nsubj We,provide
R1065 T1832 T1833 mark that,had
R1066 T1819 T1818 dobj evidence,provide
R1067 T1820 T1821 mark that,regulates
R1068 T1833 T1831 ccomp had,showing
R1069 T1821 T1819 acl regulates,evidence
R1070 T1822 T1821 nsubj Sam68,regulates
R1071 T1823 T1824 det the,differentiation
R1072 T1834 T1833 nsubj cells,had
R1073 T1824 T1821 dobj differentiation,regulates
R1074 T1825 T1824 prep of,differentiation
R1075 T1835 T1834 acl isolated,cells
R1076 T1836 T1835 prep from,isolated
R1077 T1837 T1838 nmod Sam68,animals
R1078 T1838 T1836 pobj animals,from
R1079 T1839 T1837 punct −,Sam68
R1080 T1840 T1837 punct /,Sam68
R1081 T1841 T1837 punct −,Sam68
R1082 T1842 T1843 amod enhanced,activity
R1083 T1843 T1833 dobj activity,had
R1084 T1844 T1843 amod osteogenic,activity
R1085 T1845 T1843 cc and,activity
R1086 T1846 T1847 amod decreased,activity
R1087 T1847 T1843 conj activity,activity
R1088 T1848 T1847 amod adipogenic,activity
R1089 T1849 T1843 prep than,activity
R1090 T1850 T1849 pobj those,than
R1091 T1851 T1850 acl harvested,those
R1092 T1852 T1851 prep from,harvested
R1093 T1853 T1854 amod wild,type
R1094 T1854 T1856 compound type,littermates
R1095 T1855 T1854 punct -,type
R1096 T1856 T1852 pobj littermates,from
R1097 T1857 T1818 punct .,provide
R1098 T1859 T1860 advmod Furthermore,impaired
R1099 T1861 T1860 punct ", ",impaired
R1100 T1862 T1860 nsubjpass Sam68,impaired
R1101 T1863 T1862 punct −,Sam68
R1102 T1864 T1862 punct /,Sam68
R1103 T1865 T1862 punct −,Sam68
R1104 T1866 T1867 compound mouse,embryo
R1105 T1867 T1868 compound embryo,fibroblasts
R1106 T1868 T1862 appos fibroblasts,Sam68
R1107 T1869 T1868 punct (,fibroblasts
R1108 T1870 T1868 appos MEFs,fibroblasts
R1109 T1871 T1862 punct ),Sam68
R1110 T1872 T1860 auxpass were,impaired
R1111 T1873 T1860 prep in,impaired
R1112 T1874 T1875 poss their,capacity
R1113 T1875 T1873 pobj capacity,in
R1114 T1876 T1877 aux to,differentiate
R1115 T1877 T1875 acl differentiate,capacity
R1116 T1878 T1877 prep into,differentiate
R1117 T1879 T1878 pobj adipocytes,into
R1118 T1880 T1860 punct ", ",impaired
R1119 T1881 T1860 advcl consistent,impaired
R1120 T1882 T1881 prep with,consistent
R1121 T1883 T1884 nsubj Sam68,being
R1122 T1884 T1882 pcomp being,with
R1123 T1885 T1886 det a,regulator
R1124 T1886 T1884 attr regulator,being
R1125 T1887 T1886 prep of,regulator
R1126 T1888 T1889 nmod bone,marrow
R1127 T1889 T1890 nmod marrow,differentiation
R1128 T1890 T1887 pobj differentiation,of
R1129 T1891 T1890 amod mesenchymal,differentiation
R1130 T1892 T1890 compound cell,differentiation
R1131 T1893 T1860 punct .,impaired
R1132 T1895 T1896 det These,results
R1133 T1896 T1897 nsubj results,characterize
R1134 T1898 T1897 advmod also,characterize
R1135 T1899 T1900 det a,model
R1136 T1900 T1897 dobj model,characterize
R1137 T1901 T1900 amod new,model
R1138 T1902 T1900 compound animal,model
R1139 T1903 T1904 aux to,study
R1140 T1904 T1900 advcl study,model
R1141 T1905 T1906 compound bone,metabolism
R1142 T1906 T1904 dobj metabolism,study
R1143 T1907 T1906 punct ", ",metabolism
R1144 T1908 T1906 conj regeneration,metabolism
R1145 T1909 T1908 punct ", ",regeneration
R1146 T1910 T1908 cc and,regeneration
R1147 T1911 T1908 conj repair,regeneration
R1148 T1912 T1904 prep during,study
R1149 T1913 T1912 pobj aging,during
R1150 T1914 T1897 punct .,characterize
R1155 T2061 T2062 nsubjpass Sam68,Expressed
R1156 T2063 T2062 auxpass Is,Expressed
R1157 T2064 T2062 prep in,Expressed
R1158 T2065 T2066 det the,Skeleton
R1159 T2066 T2064 pobj Skeleton,in
R1160 T2067 T2066 amod Developing,Skeleton
R1161 T2068 T2066 prep of,Skeleton
R1162 T2069 T2070 amod Embryonic,Mice
R1163 T2070 T2068 pobj Mice,of
R1164 T2072 T2073 det The,mRNA
R1165 T2073 T2075 nsubjpass mRNA,known
R1166 T2074 T2073 compound Sam68,mRNA
R1167 T2076 T2075 auxpass is,known
R1168 T2077 T2078 aux to,expressed
R1169 T2078 T2075 xcomp expressed,known
R1170 T2079 T2078 auxpass be,expressed
R1171 T2080 T2078 advmod widely,expressed
R1172 T2081 T2082 punct [,38
R1173 T2082 T2078 parataxis 38,expressed
R1174 T2083 T2082 punct ],38
R1175 T2084 T2078 punct ", ",expressed
R1176 T2085 T2086 mark whereas,remains
R1177 T2086 T2078 advcl remains,expressed
R1178 T2087 T2088 poss its,pattern
R1179 T2088 T2086 nsubj pattern,remains
R1180 T2089 T2088 prep of,pattern
R1181 T2090 T2091 compound protein,expression
R1182 T2091 T2089 pobj expression,of
R1183 T2092 T2093 advmod in,vivo
R1184 T2093 T2088 advmod vivo,pattern
R1185 T2094 T2095 aux to,defined
R1186 T2095 T2086 xcomp defined,remains
R1187 T2096 T2095 auxpass be,defined
R1188 T2097 T2075 punct .,known
R1189 T2099 T2100 nsubjpass Sections,immunostained
R1190 T2101 T2099 prep of,Sections
R1191 T2102 T2103 npadvmod paraffin,embedded
R1192 T2103 T2105 amod embedded,mice
R1193 T2104 T2103 punct -,embedded
R1194 T2105 T2101 pobj mice,of
R1195 T2106 T2107 amod wild,type
R1196 T2107 T2105 nmod type,mice
R1197 T2108 T2107 punct -,type
R1198 T2109 T2105 nmod E14.5,mice
R1199 T2110 T2109 cc and,E14.5
R1200 T2111 T2109 conj E16.5,E14.5
R1201 T2112 T2105 amod embryonic,mice
R1202 T2113 T2100 auxpass were,immunostained
R1203 T2114 T2100 prep with,immunostained
R1204 T2115 T2116 det the,antibody
R1205 T2116 T2114 pobj antibody,with
R1206 T2117 T2118 advmod well,characterized
R1207 T2118 T2116 amod characterized,antibody
R1208 T2119 T2118 punct -,characterized
R1209 T2120 T2116 nmod AD1,antibody
R1210 T2121 T2116 amod anti-Sam68,antibody
R1211 T2122 T2116 punct ", ",antibody
R1212 T2123 T2116 acl raised,antibody
R1213 T2124 T2123 prep in,raised
R1214 T2125 T2124 pobj rabbits,in
R1215 T2126 T2116 prep against,antibody
R1216 T2127 T2128 det an,peptide
R1217 T2128 T2126 pobj peptide,against
R1218 T2129 T2128 amod immunizing,peptide
R1219 T2130 T2131 dep that,corresponds
R1220 T2131 T2128 relcl corresponds,peptide
R1221 T2132 T2131 prep to,corresponds
R1222 T2133 T2134 nmod amino,330
R1223 T2134 T2132 pobj 330,to
R1224 T2135 T2134 nmod acids,330
R1225 T2136 T2134 prep to,330
R1226 T2137 T2136 pobj 348,to
R1227 T2138 T2134 prep of,330
R1228 T2139 T2140 det the,protein
R1229 T2140 T2138 pobj protein,of
R1230 T2141 T2140 compound mouse,protein
R1231 T2142 T2140 compound Sam68,protein
R1232 T2143 T2144 punct [,46
R1233 T2144 T2100 parataxis 46,immunostained
R1234 T2145 T2144 punct ],46
R1235 T2146 T2100 punct .,immunostained
R1236 T2148 T2149 compound Sam68,immunoreactivity
R1237 T2149 T2150 nsubjpass immunoreactivity,observed
R1238 T2151 T2150 auxpass was,observed
R1239 T2152 T2150 prep in,observed
R1240 T2153 T2154 det the,skeleton
R1241 T2154 T2152 pobj skeleton,in
R1242 T2155 T2154 cc and,skeleton
R1243 T2156 T2157 amod soft,tissues
R1244 T2157 T2154 conj tissues,skeleton
R1245 T2158 T2154 prep of,skeleton
R1246 T2159 T2160 amod developing,mice
R1247 T2160 T2158 pobj mice,of
R1248 T2161 T2162 amod wild,type
R1249 T2162 T2160 compound type,mice
R1250 T2163 T2162 punct -,type
R1251 T2164 T2150 prep at,observed
R1252 T2165 T2164 pobj E14.5,at
R1253 T2166 T2165 cc and,E14.5
R1254 T2167 T2165 conj E16.5,E14.5
R1255 T2168 T2169 punct (,Figure
R1256 T2169 T2150 parataxis Figure,observed
R1257 T2170 T2169 nummod 1,Figure
R1258 T2171 T2169 punct ),Figure
R1259 T2172 T2150 punct .,observed
R1260 T2174 T2175 amod Intense,staining
R1261 T2175 T2176 nsubjpass staining,seen
R1262 T2177 T2176 auxpass was,seen
R1263 T2178 T2176 prep in,seen
R1264 T2179 T2180 det the,nucleus
R1265 T2180 T2178 pobj nucleus,in
R1266 T2181 T2180 prep of,nucleus
R1267 T2182 T2181 pobj cells,of
R1268 T2183 T2176 prep in,seen
R1269 T2184 T2185 det the,brain
R1270 T2185 T2183 pobj brain,in
R1271 T2186 T2185 amod developing,brain
R1272 T2187 T2185 punct ", ",brain
R1273 T2188 T2185 conj heart,brain
R1274 T2189 T2188 punct ", ",heart
R1275 T2190 T2188 cc and,heart
R1276 T2191 T2192 amod small,intestine
R1277 T2192 T2188 conj intestine,heart
R1278 T2193 T2194 punct (,1B
R1279 T2194 T2185 parataxis 1B,brain
R1280 T2195 T2194 compound Figure,1B
R1281 T2196 T2194 punct ),1B
R1282 T2197 T2183 punct ", ",in
R1283 T2198 T2199 advmod as,as
R1284 T2199 T2183 cc as,in
R1285 T2200 T2199 advmod well,as
R1286 T2201 T2183 conj in,in
R1287 T2202 T2201 pobj chondrocytes,in
R1288 T2203 T2202 prep in,chondrocytes
R1289 T2204 T2205 det the,septum
R1290 T2205 T2203 pobj septum,in
R1291 T2206 T2205 amod nasal,septum
R1292 T2207 T2205 cc and,septum
R1293 T2208 T2209 det the,tissue
R1294 T2209 T2205 conj tissue,septum
R1295 T2210 T2209 amod glandular,tissue
R1296 T2211 T2209 amod adjacent,tissue
R1297 T2212 T2211 prep to,adjacent
R1298 T2213 T2214 det the,cartilage
R1299 T2214 T2212 pobj cartilage,to
R1300 T2215 T2214 amod nasal,cartilage
R1301 T2216 T2217 punct (,1C
R1302 T2217 T2202 parataxis 1C,chondrocytes
R1303 T2218 T2217 compound Figure,1C
R1304 T2219 T2217 punct ", ",1C
R1305 T2220 T2221 compound panels,A
R1306 T2221 T2217 appos A,1C
R1307 T2222 T2223 punct –,D
R1308 T2223 T2221 prep D,A
R1309 T2224 T2217 punct ),1C
R1310 T2225 T2201 punct ", ",in
R1311 T2226 T2201 prep in,in
R1312 T2227 T2228 det the,vertebra
R1313 T2228 T2226 pobj vertebra,in
R1314 T2229 T2228 cc and,vertebra
R1315 T2230 T2231 amod intervertebral,discs
R1316 T2231 T2228 conj discs,vertebra
R1317 T2232 T2233 punct (,E
R1318 T2233 T2228 parataxis E,vertebra
R1319 T2234 T2233 compound panels,E
R1320 T2235 T2236 punct –,H
R1321 T2236 T2233 prep H,E
R1322 T2237 T2233 punct ),E
R1323 T2238 T2226 cc and,in
R1324 T2239 T2226 conj in,in
R1325 T2240 T2241 det the,epiphysis
R1326 T2241 T2239 pobj epiphysis,in
R1327 T2242 T2243 punct (,I
R1328 T2243 T2241 parataxis I,epiphysis
R1329 T2244 T2243 compound panels,I
R1330 T2245 T2246 punct –,K
R1331 T2246 T2243 prep K,I
R1332 T2247 T2243 punct ),I
R1333 T2248 T2241 cc and,epiphysis
R1334 T2249 T2241 conj metaphysis,epiphysis
R1335 T2250 T2251 punct (,L
R1336 T2251 T2249 parataxis L,metaphysis
R1337 T2252 T2251 compound panel,L
R1338 T2253 T2251 punct ),L
R1339 T2254 T2241 prep of,epiphysis
R1340 T2255 T2256 amod long,bones
R1341 T2256 T2254 pobj bones,of
R1342 T2257 T2176 punct .,seen
R1343 T2259 T2260 amod Nuclear,staining
R1344 T2260 T2261 nsubjpass staining,observed
R1345 T2262 T2261 auxpass was,observed
R1346 T2263 T2261 prep in,observed
R1347 T2264 T2265 amod proliferating,chondrocytes
R1348 T2265 T2263 pobj chondrocytes,in
R1349 T2266 T2265 cc and,chondrocytes
R1350 T2267 T2268 amod hypertrophic,chondrocytes
R1351 T2268 T2265 conj chondrocytes,chondrocytes
R1352 T2269 T2270 punct (,1C
R1353 T2270 T2268 parataxis 1C,chondrocytes
R1354 T2271 T2270 compound Figure,1C
R1355 T2272 T2270 punct ", ",1C
R1356 T2273 T2274 compound panel,H
R1357 T2274 T2270 appos H,1C
R1358 T2275 T2270 punct ),1C
R1359 T2276 T2263 punct ", ",in
R1360 T2277 T2263 conj in,in
R1361 T2278 T2277 pobj osteoblasts,in
R1362 T2279 T2280 punct (,L
R1363 T2280 T2278 parataxis L,osteoblasts
R1364 T2281 T2280 compound panel,L
R1365 T2282 T2280 punct ;,L
R1366 T2283 T2284 compound Dataset,S1
R1367 T2284 T2280 appos S1,L
R1368 T2285 T2280 punct ),L
R1369 T2286 T2277 punct ", ",in
R1370 T2287 T2277 cc and,in
R1371 T2288 T2277 conj in,in
R1372 T2289 T2288 pobj osteoclasts,in
R1373 T2290 T2291 punct (,S1
R1374 T2291 T2289 parataxis S1,osteoclasts
R1375 T2292 T2291 compound Dataset,S1
R1376 T2293 T2291 punct ),S1
R1377 T2294 T2261 punct .,observed
R1378 T2296 T2297 det No,staining
R1379 T2297 T2298 nsubjpass staining,observed
R1380 T2299 T2298 auxpass was,observed
R1381 T2300 T2298 prep with,observed
R1382 T2301 T2302 amod preimmune,serum
R1383 T2302 T2300 pobj serum,with
R1384 T2303 T2300 cc or,with
R1385 T2304 T2305 advmod when,preadsorbed
R1386 T2305 T2300 conj preadsorbed,with
R1387 T2306 T2307 det the,antiserum
R1388 T2307 T2305 nsubjpass antiserum,preadsorbed
R1389 T2308 T2305 auxpass was,preadsorbed
R1390 T2309 T2305 prep with,preadsorbed
R1391 T2310 T2311 det the,peptide
R1392 T2311 T2309 pobj peptide,with
R1393 T2312 T2311 amod immunizing,peptide
R1394 T2313 T2314 punct (,1C
R1395 T2314 T2305 parataxis 1C,preadsorbed
R1396 T2315 T2314 compound Figure,1C
R1397 T2316 T2314 punct ", ",1C
R1398 T2317 T2318 compound panels,B
R1399 T2318 T2314 appos B,1C
R1400 T2319 T2318 punct ", ",B
R1401 T2320 T2318 conj F,B
R1402 T2321 T2320 punct ", ",F
R1403 T2322 T2320 cc and,F
R1404 T2323 T2320 conj J,F
R1405 T2324 T2314 punct ),1C
R1406 T2325 T2298 punct .,observed
R1407 T2327 T2328 advcl Taken,demonstrate
R1408 T2329 T2327 advmod together,Taken
R1409 T2330 T2328 punct ", ",demonstrate
R1410 T2331 T2332 det these,data
R1411 T2332 T2328 nsubj data,demonstrate
R1412 T2333 T2334 mark that,expressed
R1413 T2334 T2328 ccomp expressed,demonstrate
R1414 T2335 T2336 compound Sam68,protein
R1415 T2336 T2334 nsubjpass protein,expressed
R1416 T2337 T2334 auxpass is,expressed
R1417 T2338 T2334 advmod selectively,expressed
R1418 T2339 T2334 prep in,expressed
R1419 T2340 T2341 det the,embryo
R1420 T2341 T2339 pobj embryo,in
R1421 T2342 T2341 amod developing,embryo
R1422 T2343 T2341 compound mouse,embryo
R1423 T2344 T2334 punct ", ",expressed
R1424 T2345 T2334 prep with,expressed
R1425 T2346 T2347 advmod particularly,elevated
R1426 T2347 T2348 amod elevated,expression
R1427 T2348 T2345 pobj expression,with
R1428 T2349 T2348 prep in,expression
R1429 T2350 T2349 pobj cartilage,in
R1430 T2351 T2350 cc and,cartilage
R1431 T2352 T2350 conj bone,cartilage
R1432 T2353 T2328 punct .,demonstrate
R1433 T2520 T2521 nsubj Sam68,is
R1434 T2522 T2521 neg Not,is
R1435 T2523 T2521 acomp Essential,is
R1436 T2524 T2523 prep for,Essential
R1437 T2525 T2526 compound Mouse,Development
R1438 T2526 T2524 pobj Development,for
R1439 T2528 T2529 aux To,define
R1440 T2529 T2530 advcl define,generated
R1441 T2531 T2532 det the,role
R1442 T2532 T2529 dobj role,define
R1443 T2533 T2532 amod physiologic,role
R1444 T2534 T2532 prep of,role
R1445 T2535 T2534 pobj Sam68,of
R1446 T2536 T2530 punct ", ",generated
R1447 T2537 T2530 nsubj we,generated
R1448 T2538 T2539 npadvmod Sam68,deficient
R1449 T2539 T2541 amod deficient,mice
R1450 T2540 T2539 punct -,deficient
R1451 T2541 T2530 dobj mice,generated
R1452 T2542 T2530 prep by,generated
R1453 T2543 T2544 amod targeted,disruption
R1454 T2544 T2542 pobj disruption,by
R1455 T2545 T2544 prep of,disruption
R1456 T2546 T2547 nmod exons,4
R1457 T2547 T2545 pobj 4,of
R1458 T2548 T2547 cc and,4
R1459 T2549 T2547 conj 5,4
R1460 T2550 T2547 prep of,4
R1461 T2551 T2552 det the,gene
R1462 T2552 T2550 pobj gene,of
R1463 T2553 T2552 compound sam68,gene
R1464 T2554 T2547 punct ", ",4
R1465 T2555 T2556 dep which,encode
R1466 T2556 T2547 relcl encode,4
R1467 T2557 T2558 det the,region
R1468 T2558 T2556 dobj region,encode
R1469 T2559 T2558 amod functional,region
R1470 T2560 T2558 prep of,region
R1471 T2561 T2562 det the,domain
R1472 T2562 T2560 pobj domain,of
R1473 T2563 T2564 compound KH,type
R1474 T2564 T2562 compound type,domain
R1475 T2565 T2564 punct -,type
R1476 T2566 T2567 compound RNA,binding
R1477 T2567 T2562 compound binding,domain
R1478 T2568 T2569 punct (,2A
R1479 T2569 T2530 parataxis 2A,generated
R1480 T2570 T2569 compound Figure,2A
R1481 T2571 T2569 punct ),2A
R1482 T2572 T2530 punct .,generated
R1483 T2574 T2575 det The,integrity
R1484 T2575 T2576 nsubjpass integrity,verified
R1485 T2577 T2575 prep of,integrity
R1486 T2578 T2579 det the,allele
R1487 T2579 T2577 pobj allele,of
R1488 T2580 T2579 amod targeted,allele
R1489 T2581 T2576 auxpass was,verified
R1490 T2582 T2576 prep by,verified
R1491 T2583 T2584 compound Southern,blot
R1492 T2584 T2585 compound blot,analysis
R1493 T2585 T2582 pobj analysis,by
R1494 T2586 T2587 punct (,2B
R1495 T2587 T2585 parataxis 2B,analysis
R1496 T2588 T2587 compound Figure,2B
R1497 T2589 T2587 punct ),2B
R1498 T2590 T2582 cc and,by
R1499 T2591 T2582 conj by,by
R1500 T2592 T2591 pobj PCR,by
R1501 T2593 T2592 prep of,PCR
R1502 T2594 T2595 amod genomic,DNA
R1503 T2595 T2593 pobj DNA,of
R1504 T2596 T2597 punct (,data
R1505 T2597 T2592 meta data,PCR
R1506 T2598 T2597 amod unpublished,data
R1507 T2599 T2597 punct ),data
R1508 T2600 T2576 punct .,verified
R1509 T2602 T2603 compound Sam68,transcripts
R1510 T2603 T2604 nsubj transcripts,were
R1511 T2605 T2603 acl encoded,transcripts
R1512 T2606 T2605 agent by,encoded
R1513 T2607 T2608 nmod exons,1
R1514 T2608 T2606 pobj 1,by
R1515 T2609 T2608 prep to,1
R1516 T2610 T2609 pobj 5,to
R1517 T2611 T2604 acomp absent,were
R1518 T2612 T2613 mark as,evidenced
R1519 T2613 T2604 advcl evidenced,were
R1520 T2614 T2613 prep by,evidenced
R1521 T2615 T2616 compound RT,PCR
R1522 T2616 T2614 pobj PCR,by
R1523 T2617 T2616 punct -,PCR
R1524 T2618 T2619 punct (,S2
R1525 T2619 T2613 parataxis S2,evidenced
R1526 T2620 T2619 compound Dataset,S2
R1527 T2621 T2619 punct ),S2
R1528 T2622 T2604 punct ", ",were
R1529 T2623 T2604 cc and,were
R1530 T2624 T2625 nmod Sam68,mice
R1531 T2625 T2629 nsubj mice,were
R1532 T2626 T2624 punct −,Sam68
R1533 T2627 T2624 punct /,Sam68
R1534 T2628 T2624 punct −,Sam68
R1535 T2629 T2604 conj were,were
R1536 T2630 T2629 acomp devoid,were
R1537 T2631 T2630 prep of,devoid
R1538 T2632 T2633 compound Sam68,protein
R1539 T2633 T2631 pobj protein,of
R1540 T2634 T2629 punct ", ",were
R1541 T2635 T2636 mark as,visualized
R1542 T2636 T2629 advcl visualized,were
R1543 T2637 T2636 prep by,visualized
R1544 T2638 T2637 pcomp immunoblotting,by
R1545 T2639 T2638 prep with,immunoblotting
R1546 T2640 T2641 amod anti-Sam68,AD1
R1547 T2641 T2642 nmod AD1,antibodies
R1548 T2642 T2639 pobj antibodies,with
R1549 T2643 T2641 cc and,AD1
R1550 T2644 T2641 conj SC,AD1
R1551 T2645 T2644 punct -,SC
R1552 T2646 T2644 nummod 333,SC
R1553 T2647 T2642 cc or,antibodies
R1554 T2648 T2649 nmod control,serum
R1555 T2649 T2642 conj serum,antibodies
R1556 T2650 T2649 amod normal,serum
R1557 T2651 T2649 compound rabbit,serum
R1558 T2652 T2649 cc or,serum
R1559 T2653 T2654 amod anti-Sam68,AD1
R1560 T2654 T2649 conj AD1,serum
R1561 T2655 T2654 acl preabsorbed,AD1
R1562 T2656 T2655 prep with,preabsorbed
R1563 T2657 T2656 pobj peptide,with
R1564 T2658 T2659 punct (,2C
R1565 T2659 T2629 parataxis 2C,were
R1566 T2660 T2659 compound Figure,2C
R1567 T2661 T2659 punct ),2C
R1568 T2662 T2629 punct .,were
R1569 T2664 T2665 nsubj SC,is
R1570 T2666 T2664 punct -,SC
R1571 T2667 T2664 nummod 333,SC
R1572 T2668 T2669 det a,antibody
R1573 T2669 T2665 attr antibody,is
R1574 T2670 T2669 nmod rabbit,antibody
R1575 T2671 T2669 amod anti-Sam68,antibody
R1576 T2672 T2673 dep that,raised
R1577 T2673 T2669 relcl raised,antibody
R1578 T2674 T2673 auxpass was,raised
R1579 T2675 T2673 prep against,raised
R1580 T2676 T2677 det the,acids
R1581 T2677 T2675 pobj acids,against
R1582 T2678 T2679 npadvmod C,terminal
R1583 T2679 T2677 amod terminal,acids
R1584 T2680 T2679 punct -,terminal
R1585 T2681 T2677 nummod 20,acids
R1586 T2682 T2677 compound amino,acids
R1587 T2683 T2677 prep of,acids
R1588 T2684 T2683 pobj Sam68,of
R1589 T2685 T2686 punct [,47
R1590 T2686 T2665 parataxis 47,is
R1591 T2687 T2686 punct ],47
R1592 T2688 T2665 punct .,is
R1593 T2690 T2691 det These,data
R1594 T2691 T2692 nsubj data,confirmed
R1595 T2693 T2694 det the,generation
R1596 T2694 T2692 dobj generation,confirmed
R1597 T2695 T2694 prep of,generation
R1598 T2696 T2697 det a,mouse
R1599 T2697 T2695 pobj mouse,of
R1600 T2698 T2697 amod deficient,mouse
R1601 T2699 T2698 prep in,deficient
R1602 T2700 T2699 pobj Sam68,in
R1603 T2701 T2692 punct .,confirmed
R1604 T2703 T2704 det The,genotypes
R1605 T2704 T2705 nsubj genotypes,exhibited
R1606 T2706 T2704 prep of,genotypes
R1607 T2707 T2706 pobj offspring,of
R1608 T2708 T2707 prep from,offspring
R1609 T2709 T2710 compound heterozygote,intercrosses
R1610 T2710 T2708 pobj intercrosses,from
R1611 T2711 T2712 det a,segregation
R1612 T2712 T2705 dobj segregation,exhibited
R1613 T2713 T2712 amod Mendelian,segregation
R1614 T2714 T2705 prep at,exhibited
R1615 T2715 T2714 pobj E18.5,at
R1616 T2716 T2717 punct (,Table
R1617 T2717 T2705 parataxis Table,exhibited
R1618 T2718 T2717 nummod 1,Table
R1619 T2719 T2717 punct ),Table
R1620 T2720 T2705 punct .,exhibited
R1621 T2722 T2723 prep Despite,died
R1622 T2724 T2725 det the,lack
R1623 T2725 T2722 pobj lack,Despite
R1624 T2726 T2725 prep of,lack
R1625 T2727 T2728 amod visible,deformity
R1626 T2728 T2726 pobj deformity,of
R1627 T2729 T2723 punct ", ",died
R1628 T2730 T2723 nsubj many,died
R1629 T2731 T2730 prep of,many
R1630 T2732 T2733 det the,pups
R1631 T2733 T2731 pobj pups,of
R1632 T2734 T2733 nmod Sam68,pups
R1633 T2735 T2734 punct −,Sam68
R1634 T2736 T2734 punct /,Sam68
R1635 T2737 T2734 punct −,Sam68
R1636 T2738 T2723 prep at,died
R1637 T2739 T2738 pobj birth,at
R1638 T2740 T2723 prep of,died
R1639 T2741 T2742 amod unknown,causes
R1640 T2742 T2740 pobj causes,of
R1641 T2743 T2723 punct .,died
R1642 T2745 T2746 nmod Sam68,mice
R1643 T2746 T2750 nsubj mice,were
R1644 T2747 T2745 punct +,Sam68
R1645 T2748 T2745 punct /,Sam68
R1646 T2749 T2745 punct -,Sam68
R1647 T2751 T2752 advmod phenotypically,normal
R1648 T2752 T2750 acomp normal,were
R1649 T2753 T2750 cc and,were
R1650 T2754 T2755 nmod Sam68,pups
R1651 T2755 T2759 nsubj pups,lived
R1652 T2756 T2754 punct −,Sam68
R1653 T2757 T2754 punct /,Sam68
R1654 T2758 T2754 punct −,Sam68
R1655 T2759 T2750 conj lived,were
R1656 T2760 T2761 dep that,survived
R1657 T2761 T2755 relcl survived,pups
R1658 T2762 T2763 det the,period
R1659 T2763 T2761 dobj period,survived
R1660 T2764 T2763 amod perinatal,period
R1661 T2765 T2759 advmod invariably,lived
R1662 T2766 T2759 prep to,lived
R1663 T2767 T2768 amod old,age
R1664 T2768 T2766 pobj age,to
R1665 T2769 T2759 punct .,lived
R1666 T2771 T2772 prep Despite,develop
R1667 T2773 T2771 pobj evidence,Despite
R1668 T2774 T2775 mark that,expressed
R1669 T2775 T2773 acl expressed,evidence
R1670 T2776 T2777 compound Sam68,mRNA
R1671 T2777 T2775 nsubjpass mRNA,expressed
R1672 T2778 T2775 auxpass is,expressed
R1673 T2779 T2775 advmod widely,expressed
R1674 T2780 T2775 cc and,expressed
R1675 T2781 T2775 conj phosphorylated,expressed
R1676 T2782 T2781 prep in,phosphorylated
R1677 T2783 T2782 pobj mitosis,in
R1678 T2784 T2785 punct [,42
R1679 T2785 T2775 parataxis 42,expressed
R1680 T2786 T2785 nummod 41,42
R1681 T2787 T2785 punct ",",42
R1682 T2788 T2785 punct ],42
R1683 T2789 T2772 punct ", ",develop
R1684 T2790 T2791 det the,mice
R1685 T2791 T2772 nsubj mice,develop
R1686 T2792 T2791 nmod Sam68,mice
R1687 T2793 T2792 punct −,Sam68
R1688 T2794 T2792 punct /,Sam68
R1689 T2795 T2792 punct −,Sam68
R1690 T2796 T2772 aux did,develop
R1691 T2797 T2772 neg not,develop
R1692 T2798 T2772 dobj tumors,develop
R1693 T2799 T2772 cc and,develop
R1694 T2800 T2772 conj showed,develop
R1695 T2801 T2802 det no,illnesses
R1696 T2802 T2800 dobj illnesses,showed
R1697 T2803 T2802 amod immunologic,illnesses
R1698 T2804 T2803 cc or,immunologic
R1699 T2805 T2806 amod other,major
R1700 T2806 T2803 conj major,immunologic
R1701 T2807 T2772 punct .,develop
R1702 T2809 T2810 nmod Sam68,mice
R1703 T2810 T2814 nsubj mice,have
R1704 T2811 T2809 punct −,Sam68
R1705 T2812 T2809 punct /,Sam68
R1706 T2813 T2809 punct −,Sam68
R1707 T2815 T2814 aux did,have
R1708 T2816 T2814 punct ", ",have
R1709 T2817 T2814 advmod however,have
R1710 T2818 T2814 punct ", ",have
R1711 T2819 T2814 dobj difficulty,have
R1712 T2820 T2819 acl breeding,difficulty
R1713 T2821 T2814 prep due,have
R1714 T2822 T2821 pcomp to,due
R1715 T2823 T2824 amod male,infertility
R1716 T2824 T2821 pobj infertility,due
R1717 T2825 T2814 cc and,have
R1718 T2826 T2827 det the,females
R1719 T2827 T2828 nsubj females,provided
R1720 T2828 T2814 conj provided,have
R1721 T2829 T2828 advmod rarely,provided
R1722 T2830 T2831 amod adequate,care
R1723 T2831 T2828 dobj care,provided
R1724 T2832 T2828 dative to,provided
R1725 T2833 T2834 poss their,young
R1726 T2834 T2832 pobj young,to
R1727 T2835 T2828 punct .,provided
R1732 T3035 T3036 compound Sam68,Deficiency
R1733 T3036 T3037 nsubj Deficiency,Protects
R1734 T3038 T3037 dobj Mice,Protects
R1735 T3039 T3037 prep from,Protects
R1736 T3040 T3041 npadvmod Age,Related
R1737 T3041 T3043 amod Related,Loss
R1738 T3042 T3041 punct -,Related
R1739 T3043 T3039 pobj Loss,from
R1740 T3044 T3043 compound Bone,Loss
R1741 T3046 T3047 nmod Src,animals
R1742 T3047 T3049 nsubjpass animals,known
R1743 T3048 T3047 amod null,animals
R1744 T3050 T3049 auxpass are,known
R1745 T3051 T3052 aux to,have
R1746 T3052 T3049 xcomp have,known
R1747 T3053 T3054 compound bone,metabolism
R1748 T3054 T3055 compound metabolism,defects
R1749 T3055 T3052 dobj defects,have
R1750 T3056 T3057 punct [,48
R1751 T3057 T3049 parataxis 48,known
R1752 T3058 T3057 punct ],48
R1753 T3059 T3049 punct ", ",known
R1754 T3060 T3049 cc and,known
R1755 T3061 T3062 mark since,is
R1756 T3062 T3064 advcl is,decided
R1757 T3063 T3062 nsubj Sam68,is
R1758 T3064 T3049 conj decided,known
R1759 T3065 T3066 det a,substrate
R1760 T3066 T3062 attr substrate,is
R1761 T3067 T3066 prep of,substrate
R1762 T3068 T3067 pobj Src,of
R1763 T3069 T3064 punct ", ",decided
R1764 T3070 T3064 nsubj we,decided
R1765 T3071 T3072 aux to,analyze
R1766 T3072 T3064 xcomp analyze,decided
R1767 T3073 T3074 det the,mice
R1768 T3074 T3072 dobj mice,analyze
R1769 T3075 T3074 compound Sam68,mice
R1770 T3076 T3072 prep for,analyze
R1771 T3077 T3078 amod skeletal,abnormalities
R1772 T3078 T3076 pobj abnormalities,for
R1773 T3079 T3064 punct .,decided
R1774 T3081 T3082 nsubjpass Cohorts,euthanized
R1775 T3083 T3081 prep of,Cohorts
R1776 T3084 T3085 nmod Sam68,mice
R1777 T3085 T3083 pobj mice,of
R1778 T3086 T3084 punct +,Sam68
R1779 T3087 T3084 punct /,Sam68
R1780 T3088 T3084 punct +,Sam68
R1781 T3089 T3084 cc and,Sam68
R1782 T3090 T3084 conj Sam68,Sam68
R1783 T3091 T3090 punct −,Sam68
R1784 T3092 T3090 punct /,Sam68
R1785 T3093 T3090 punct −,Sam68
R1786 T3094 T3082 auxpass were,euthanized
R1787 T3095 T3082 prep at,euthanized
R1788 T3096 T3097 nummod 4,months
R1789 T3097 T3095 pobj months,at
R1790 T3098 T3096 cc and,4
R1791 T3099 T3096 conj 12,4
R1792 T3100 T3097 prep of,months
R1793 T3101 T3100 pobj age,of
R1794 T3102 T3082 prep for,euthanized
R1795 T3103 T3104 amod skeletal,phenotyping
R1796 T3104 T3102 pobj phenotyping,for
R1797 T3105 T3082 punct .,euthanized
R1798 T3107 T3108 aux To,minimize
R1799 T3108 T3109 advcl minimize,selected
R1800 T3110 T3108 dobj differences,minimize
R1801 T3111 T3110 prep in,differences
R1802 T3112 T3113 det the,phenotype
R1803 T3113 T3111 pobj phenotype,in
R1804 T3114 T3113 compound bone,phenotype
R1805 T3115 T3116 dep that,arise
R1806 T3116 T3110 relcl arise,differences
R1807 T3117 T3116 aux might,arise
R1808 T3118 T3116 advcl secondary,arise
R1809 T3119 T3118 prep to,secondary
R1810 T3120 T3121 nmod gender,differences
R1811 T3121 T3119 pobj differences,to
R1812 T3122 T3120 cc or,gender
R1813 T3123 T3120 conj weight,gender
R1814 T3124 T3109 punct ", ",selected
R1815 T3125 T3109 nsubj we,selected
R1816 T3126 T3127 npadvmod age,matched
R1817 T3127 T3129 amod matched,mice
R1818 T3128 T3127 punct -,matched
R1819 T3129 T3109 dobj mice,selected
R1820 T3130 T3129 amod female,mice
R1821 T3131 T3109 prep for,selected
R1822 T3132 T3133 det these,analyses
R1823 T3133 T3131 pobj analyses,for
R1824 T3134 T3109 punct .,selected
R1825 T3136 T3137 det The,mice
R1826 T3137 T3139 nsubj mice,demonstrated
R1827 T3138 T3137 amod female,mice
R1828 T3140 T3141 amod similar,increases
R1829 T3141 T3139 dobj increases,demonstrated
R1830 T3142 T3141 prep in,increases
R1831 T3143 T3144 compound body,weight
R1832 T3144 T3142 pobj weight,in
R1833 T3145 T3146 punct ", ",weighed
R1834 T3146 T3141 parataxis weighed,increases
R1835 T3147 T3146 mark although,weighed
R1836 T3148 T3149 nummod 4,month
R1837 T3149 T3151 npadvmod month,old
R1838 T3150 T3149 punct -,month
R1839 T3151 T3153 amod old,mice
R1840 T3152 T3151 punct -,old
R1841 T3153 T3146 nsubj mice,weighed
R1842 T3154 T3153 nmod Sam68,mice
R1843 T3155 T3154 punct −,Sam68
R1844 T3156 T3154 punct /,Sam68
R1845 T3157 T3154 punct −,Sam68
R1846 T3158 T3159 amod less,than
R1847 T3159 T3146 prep than,weighed
R1848 T3160 T3161 nmod Sam68,mice
R1849 T3161 T3159 pobj mice,than
R1850 T3162 T3160 punct +,Sam68
R1851 T3163 T3160 punct /,Sam68
R1852 T3164 T3160 punct +,Sam68
R1853 T3165 T3141 punct ", ",increases
R1854 T3166 T3141 cc and,increases
R1855 T3167 T3168 amod similar,changes
R1856 T3168 T3141 conj changes,increases
R1857 T3169 T3168 prep in,changes
R1858 T3170 T3171 compound bone,lengths
R1859 T3171 T3169 pobj lengths,in
R1860 T3172 T3168 prep in,changes
R1861 T3173 T3174 det the,skeleton
R1862 T3174 T3172 pobj skeleton,in
R1863 T3175 T3174 amod axial,skeleton
R1864 T3176 T3175 cc and,axial
R1865 T3177 T3175 conj appendicular,axial
R1866 T3178 T3168 prep between,changes
R1867 T3179 T3180 nummod 4,months
R1868 T3180 T3178 pobj months,between
R1869 T3181 T3179 cc and,4
R1870 T3182 T3179 conj 12,4
R1871 T3183 T3180 prep of,months
R1872 T3184 T3183 pobj age,of
R1873 T3185 T3186 punct (,Table
R1874 T3186 T3139 parataxis Table,demonstrated
R1875 T3187 T3186 nummod 2,Table
R1876 T3188 T3186 punct ),Table
R1877 T3189 T3139 punct .,demonstrated
R1878 T3191 T3192 compound Faxitron,radiography
R1879 T3192 T3193 nsubj radiography,revealed
R1880 T3194 T3195 punct (,Corporation
R1881 T3195 T3192 parataxis Corporation,radiography
R1882 T3196 T3195 compound Faxitron,Corporation
R1883 T3197 T3198 compound X,ray
R1884 T3198 T3195 compound ray,Corporation
R1885 T3199 T3198 punct -,ray
R1886 T3200 T3195 punct ", ",Corporation
R1887 T3201 T3195 npadvmod Wheeling,Corporation
R1888 T3202 T3195 punct ", ",Corporation
R1889 T3203 T3195 npadvmod Illinois,Corporation
R1890 T3204 T3195 punct ", ",Corporation
R1891 T3205 T3206 compound United,States
R1892 T3206 T3195 npadvmod States,Corporation
R1893 T3207 T3195 punct ),Corporation
R1894 T3208 T3209 punct (,3A
R1895 T3209 T3192 parataxis 3A,radiography
R1896 T3210 T3209 compound Figure,3A
R1897 T3211 T3209 punct ),3A
R1898 T3212 T3192 cc and,radiography
R1899 T3213 T3214 amod micro–computed,tomography
R1900 T3214 T3192 conj tomography,radiography
R1901 T3215 T3216 punct (,CT
R1902 T3216 T3214 parataxis CT,tomography
R1903 T3217 T3216 punct ),CT
R1904 T3218 T3219 punct (,3B
R1905 T3219 T3214 parataxis 3B,tomography
R1906 T3220 T3219 compound Figure,3B
R1907 T3221 T3219 punct ),3B
R1908 T3222 T3223 amod significant,thinning
R1909 T3223 T3193 dobj thinning,revealed
R1910 T3224 T3223 amod cortical,thinning
R1911 T3225 T3226 punct (,arrow
R1912 T3226 T3223 parataxis arrow,thinning
R1913 T3227 T3226 punct ),arrow
R1914 T3228 T3223 cc and,thinning
R1915 T3229 T3230 det a,reduction
R1916 T3230 T3223 conj reduction,thinning
R1917 T3231 T3230 prep in,reduction
R1918 T3232 T3233 amod metaphyseal,bone
R1919 T3233 T3231 pobj bone,in
R1920 T3234 T3235 punct (,asterisk
R1921 T3235 T3230 parataxis asterisk,reduction
R1922 T3236 T3235 punct ),asterisk
R1923 T3237 T3223 prep in,thinning
R1924 T3238 T3239 det the,femora
R1925 T3239 T3237 pobj femora,in
R1926 T3240 T3239 amod distal,femora
R1927 T3241 T3239 prep of,femora
R1928 T3242 T3243 nummod 12,month
R1929 T3243 T3245 npadvmod month,old
R1930 T3244 T3243 punct -,month
R1931 T3245 T3247 amod old,mice
R1932 T3246 T3245 punct -,old
R1933 T3247 T3241 pobj mice,of
R1934 T3248 T3247 nmod Sam68,mice
R1935 T3249 T3248 punct +,Sam68
R1936 T3250 T3248 punct /,Sam68
R1937 T3251 T3248 punct +,Sam68
R1938 T3252 T3223 prep compared,thinning
R1939 T3253 T3252 prep with,compared
R1940 T3254 T3255 nummod 4,month
R1941 T3255 T3257 npadvmod month,old
R1942 T3256 T3255 punct -,month
R1943 T3257 T3259 amod old,mice
R1944 T3258 T3257 punct -,old
R1945 T3259 T3253 pobj mice,with
R1946 T3260 T3259 prep of,mice
R1947 T3261 T3262 preconj either,genotype
R1948 T3262 T3260 pobj genotype,of
R1949 T3263 T3259 cc and,mice
R1950 T3264 T3265 nummod 12,month
R1951 T3265 T3267 npadvmod month,old
R1952 T3266 T3265 punct -,month
R1953 T3267 T3269 amod old,mice
R1954 T3268 T3267 punct -,old
R1955 T3269 T3259 conj mice,mice
R1956 T3270 T3269 nmod Sam68,mice
R1957 T3271 T3270 punct −,Sam68
R1958 T3272 T3270 punct /,Sam68
R1959 T3273 T3270 punct −,Sam68
R1960 T3274 T3193 punct .,revealed
R1961 T3276 T3277 amod Similar,reductions
R1962 T3277 T3278 nsubjpass reductions,shown
R1963 T3279 T3277 prep in,reductions
R1964 T3280 T3281 amod trabecular,bone
R1965 T3281 T3279 pobj bone,in
R1966 T3282 T3278 auxpass were,shown
R1967 T3283 T3278 prep by,shown
R1968 T3284 T3283 pobj Faxitron,by
R1969 T3285 T3284 cc and,Faxitron
R1970 T3286 T3284 conj micro-CT,Faxitron
R1971 T3287 T3278 prep in,shown
R1972 T3288 T3289 det the,vertebrae
R1973 T3289 T3287 pobj vertebrae,in
R1974 T3290 T3289 amod fifth,vertebrae
R1975 T3291 T3289 compound lumbar,vertebrae
R1976 T3292 T3289 prep of,vertebrae
R1977 T3293 T3294 det the,mice
R1978 T3294 T3292 pobj mice,of
R1979 T3295 T3296 nummod 12,month
R1980 T3296 T3298 npadvmod month,old
R1981 T3297 T3296 punct -,month
R1982 T3298 T3294 amod old,mice
R1983 T3299 T3298 punct -,old
R1984 T3300 T3294 nmod Sam68,mice
R1985 T3301 T3300 punct +,Sam68
R1986 T3302 T3300 punct /,Sam68
R1987 T3303 T3300 punct +,Sam68
R1988 T3304 T3287 cc but,in
R1989 T3305 T3304 neg not,but
R1990 T3306 T3287 conj in,in
R1991 T3307 T3308 det the,mice
R1992 T3308 T3306 pobj mice,in
R1993 T3309 T3308 nmod Sam68,mice
R1994 T3310 T3309 punct −,Sam68
R1995 T3311 T3309 punct /,Sam68
R1996 T3312 T3309 punct −,Sam68
R1997 T3313 T3314 punct (,data
R1998 T3314 T3278 meta data,shown
R1999 T3315 T3314 amod unpublished,data
R2000 T3316 T3314 punct ),data
R2001 T3317 T3278 punct .,shown
R2002 T3319 T3320 amod Total,content
R2003 T3320 T3324 nsubj content,increased
R2004 T3321 T3320 compound body,content
R2005 T3322 T3320 compound bone,content
R2006 T3323 T3320 compound mineral,content
R2007 T3325 T3320 punct (,content
R2008 T3326 T3320 appos BMC,content
R2009 T3327 T3320 punct ),content
R2010 T3328 T3320 punct ", ",content
R2011 T3329 T3320 acl quantified,content
R2012 T3330 T3329 prep with,quantified
R2013 T3331 T3332 det a,densitometer
R2014 T3332 T3330 pobj densitometer,with
R2015 T3333 T3334 amod Lunar,PIXImus
R2016 T3334 T3332 compound PIXImus,densitometer
R2017 T3335 T3332 compound mouse,densitometer
R2018 T3336 T3337 punct (,Lunar
R2019 T3337 T3332 parataxis Lunar,densitometer
R2020 T3338 T3337 compound GE,Lunar
R2021 T3339 T3337 punct -,Lunar
R2022 T3340 T3337 punct ", ",Lunar
R2023 T3341 T3337 npadvmod Madison,Lunar
R2024 T3342 T3337 punct ", ",Lunar
R2025 T3343 T3337 npadvmod Wisconsin,Lunar
R2026 T3344 T3337 punct ", ",Lunar
R2027 T3345 T3346 compound United,States
R2028 T3346 T3337 npadvmod States,Lunar
R2029 T3347 T3337 punct ),Lunar
R2030 T3348 T3324 punct ", ",increased
R2031 T3349 T3324 prep in,increased
R2032 T3350 T3351 preconj both,Sam68
R2033 T3351 T3352 nmod Sam68,mice
R2034 T3352 T3349 pobj mice,in
R2035 T3353 T3351 punct +,Sam68
R2036 T3354 T3351 punct /,Sam68
R2037 T3355 T3351 punct +,Sam68
R2038 T3356 T3351 cc and,Sam68
R2039 T3357 T3351 conj Sam68,Sam68
R2040 T3358 T3357 punct −,Sam68
R2041 T3359 T3357 punct /,Sam68
R2042 T3360 T3357 punct −,Sam68
R2043 T3361 T3352 prep between,mice
R2044 T3362 T3363 nummod 4,months
R2045 T3363 T3361 pobj months,between
R2046 T3364 T3363 cc and,months
R2047 T3365 T3366 nummod 12,months
R2048 T3366 T3363 conj months,months
R2049 T3367 T3363 prep of,months
R2050 T3368 T3367 pobj age,of
R2051 T3369 T3324 punct ", ",increased
R2052 T3370 T3324 cc but,increased
R2053 T3371 T3372 advmod only,reached
R2054 T3372 T3324 conj reached,increased
R2055 T3373 T3372 dobj significance,reached
R2056 T3374 T3372 prep in,reached
R2057 T3375 T3376 det the,mice
R2058 T3376 T3374 pobj mice,in
R2059 T3377 T3376 nmod Sam68,mice
R2060 T3378 T3377 punct −,Sam68
R2061 T3379 T3377 punct /,Sam68
R2062 T3380 T3377 punct −,Sam68
R2063 T3381 T3382 punct (,Table
R2064 T3382 T3372 parataxis Table,reached
R2065 T3383 T3382 nummod 2,Table
R2066 T3384 T3382 punct ),Table
R2067 T3385 T3324 punct .,increased
R2068 T3387 T3388 advmod Similarly,seen
R2069 T3389 T3388 punct ", ",seen
R2070 T3390 T3391 det a,increase
R2071 T3391 T3388 nsubjpass increase,seen
R2072 T3392 T3391 amod greater,increase
R2073 T3393 T3391 prep in,increase
R2074 T3394 T3393 pobj BMC,in
R2075 T3395 T3388 auxpass was,seen
R2076 T3396 T3388 prep in,seen
R2077 T3397 T3398 det the,femur
R2078 T3398 T3396 pobj femur,in
R2079 T3399 T3398 cc and,femur
R2080 T3400 T3398 conj vertebra,femur
R2081 T3401 T3398 prep in,femur
R2082 T3402 T3403 det the,mice
R2083 T3403 T3401 pobj mice,in
R2084 T3404 T3403 nmod Sam68,mice
R2085 T3405 T3404 punct −,Sam68
R2086 T3406 T3404 punct /,Sam68
R2087 T3407 T3404 punct −,Sam68
R2088 T3408 T3388 punct .,seen
R2089 T3410 T3411 compound Bone,mineral
R2090 T3411 T3412 compound mineral,density
R2091 T3412 T3413 nsubj density,remained
R2092 T3414 T3412 punct (,density
R2093 T3415 T3412 appos BMD,density
R2094 T3416 T3413 punct ),remained
R2095 T3417 T3413 acomp constant,remained
R2096 T3418 T3413 cc or,remained
R2097 T3419 T3413 conj decreased,remained
R2098 T3420 T3413 prep in,remained
R2099 T3421 T3422 det the,mice
R2100 T3422 T3420 pobj mice,in
R2101 T3423 T3424 amod wild,type
R2102 T3424 T3422 compound type,mice
R2103 T3425 T3413 cc but,remained
R2104 T3426 T3413 conj increased,remained
R2105 T3427 T3426 advmod significantly,increased
R2106 T3428 T3426 prep in,increased
R2107 T3429 T3430 det the,body
R2108 T3430 T3428 pobj body,in
R2109 T3431 T3430 amod total,body
R2110 T3432 T3428 cc and,in
R2111 T3433 T3428 conj in,in
R2112 T3434 T3435 det the,regions
R2113 T3435 T3433 pobj regions,in
R2114 T3436 T3435 amod femoral,regions
R2115 T3437 T3436 cc and,femoral
R2116 T3438 T3436 conj vertebral,femoral
R2117 T3439 T3435 prep of,regions
R2118 T3440 T3439 pobj interest,of
R2119 T3441 T3426 prep in,increased
R2120 T3442 T3443 det the,Sam68
R2121 T3443 T3441 pobj Sam68,in
R2122 T3444 T3443 punct −,Sam68
R2123 T3445 T3443 punct /,Sam68
R2124 T3446 T3443 punct −,Sam68
R2125 T3447 T3448 punct (,Table
R2126 T3448 T3426 parataxis Table,increased
R2127 T3449 T3448 nummod 2,Table
R2128 T3450 T3448 punct ),Table
R2129 T3451 T3413 punct .,remained
R2130 T3453 T3454 det These,data
R2131 T3454 T3455 nsubj data,showed
R2132 T3456 T3457 mark that,continued
R2133 T3457 T3455 ccomp continued,showed
R2134 T3458 T3459 nmod Sam68,mice
R2135 T3459 T3457 nsubj mice,continued
R2136 T3460 T3458 punct −,Sam68
R2137 T3461 T3458 punct /,Sam68
R2138 T3462 T3458 punct −,Sam68
R2139 T3463 T3464 aux to,thrive
R2140 T3464 T3457 xcomp thrive,continued
R2141 T3465 T3464 cc and,thrive
R2142 T3466 T3464 conj accrue,thrive
R2143 T3467 T3466 dobj bone,accrue
R2144 T3468 T3466 prep in,accrue
R2145 T3469 T3470 det the,skeleton
R2146 T3470 T3468 pobj skeleton,in
R2147 T3471 T3470 amod axial,skeleton
R2148 T3472 T3471 cc and,axial
R2149 T3473 T3471 conj appendicular,axial
R2150 T3474 T3464 prep for,thrive
R2151 T3475 T3476 amod longer,12
R2152 T3476 T3478 nummod 12,months
R2153 T3477 T3476 quantmod than,12
R2154 T3478 T3474 pobj months,for
R2155 T3479 T3455 punct .,showed
R2156 T3481 T3482 nsubj This,was
R2157 T3483 T3482 prep in,was
R2158 T3484 T3483 pobj contrast,in
R2159 T3485 T3484 prep to,contrast
R2160 T3486 T3487 det the,situation
R2161 T3487 T3485 pobj situation,to
R2162 T3488 T3487 prep in,situation
R2163 T3489 T3490 npadvmod age,matched
R2164 T3490 T3492 amod matched,controls
R2165 T3491 T3490 punct -,matched
R2166 T3492 T3488 pobj controls,in
R2167 T3493 T3492 punct ", ",controls
R2168 T3494 T3492 compound littermate,controls
R2169 T3495 T3496 prep in,lost
R2170 T3496 T3487 relcl lost,situation
R2171 T3497 T3495 pobj which,in
R2172 T3498 T3499 det a,amount
R2173 T3499 T3496 nsubjpass amount,lost
R2174 T3500 T3499 amod significant,amount
R2175 T3501 T3499 prep of,amount
R2176 T3502 T3501 pobj bone,of
R2177 T3503 T3496 auxpass was,lost
R2178 T3504 T3496 prep over,lost
R2179 T3505 T3506 det the,timeframe
R2180 T3506 T3504 pobj timeframe,over
R2181 T3507 T3506 amod same,timeframe
R2182 T3508 T3482 punct .,was
R2183 T3587 T3588 advmod Three,Dimensional
R2184 T3588 T3590 amod Dimensional,Architecture
R2185 T3589 T3588 punct -,Dimensional
R2186 T3590 T3591 nsubjpass Architecture,Preserved
R2187 T3592 T3590 prep of,Architecture
R2188 T3593 T3592 pobj Bone,of
R2189 T3594 T3591 auxpass Is,Preserved
R2190 T3595 T3591 prep in,Preserved
R2191 T3596 T3597 amod Aged,Mice
R2192 T3597 T3595 pobj Mice,in
R2193 T3598 T3597 nmod Sam68,Mice
R2194 T3599 T3598 punct −,Sam68
R2195 T3600 T3598 punct /,Sam68
R2196 T3601 T3598 punct −,Sam68
R2197 T3603 T3604 compound Bone,loss
R2198 T3604 T3605 nsubj loss,are
R2199 T3606 T3604 cc and,loss
R2200 T3607 T3608 amod compromised,architecture
R2201 T3608 T3604 conj architecture,loss
R2202 T3609 T3610 amod characteristic,features
R2203 T3610 T3605 attr features,are
R2204 T3611 T3610 prep of,features
R2205 T3612 T3613 det the,skeletons
R2206 T3613 T3611 pobj skeletons,of
R2207 T3614 T3613 prep of,skeletons
R2208 T3615 T3616 amod aged,mice
R2209 T3616 T3614 pobj mice,of
R2210 T3617 T3616 nmod C57BL,mice
R2211 T3618 T3617 punct /,C57BL
R2212 T3619 T3617 nummod 6,C57BL
R2213 T3620 T3605 cc and,are
R2214 T3621 T3605 conj resemble,are
R2215 T3622 T3623 det the,features
R2216 T3623 T3621 dobj features,resemble
R2217 T3624 T3623 amod clinical,features
R2218 T3625 T3623 prep of,features
R2219 T3626 T3627 npadvmod age,related
R2220 T3627 T3629 amod related,loss
R2221 T3628 T3627 punct -,related
R2222 T3629 T3625 pobj loss,of
R2223 T3630 T3629 compound bone,loss
R2224 T3631 T3629 prep in,loss
R2225 T3632 T3631 pobj humans,in
R2226 T3633 T3634 dep that,predispose
R2227 T3634 T3623 relcl predispose,features
R2228 T3635 T3634 aux can,predispose
R2229 T3636 T3637 det an,individual
R2230 T3637 T3634 dobj individual,predispose
R2231 T3638 T3634 prep to,predispose
R2232 T3639 T3638 pobj fracture,to
R2233 T3640 T3605 punct .,are
R2234 T3642 T3643 nsubj Quantification,confirmed
R2235 T3644 T3642 prep of,Quantification
R2236 T3645 T3646 det the,data
R2237 T3646 T3644 pobj data,of
R2238 T3647 T3646 compound micro-CT,data
R2239 T3648 T3646 acl shown,data
R2240 T3649 T3648 prep in,shown
R2241 T3650 T3651 compound Figure,3B
R2242 T3651 T3649 pobj 3B,in
R2243 T3652 T3653 det the,reduction
R2244 T3653 T3643 dobj reduction,confirmed
R2245 T3654 T3653 prep in,reduction
R2246 T3655 T3656 compound bone,volume
R2247 T3656 T3654 pobj volume,in
R2248 T3657 T3653 prep compared,reduction
R2249 T3658 T3657 prep with,compared
R2250 T3659 T3660 compound tissue,volume
R2251 T3660 T3658 pobj volume,with
R2252 T3661 T3662 punct (,4A
R2253 T3662 T3660 parataxis 4A,volume
R2254 T3663 T3664 compound BV,TV
R2255 T3664 T3662 dep TV,4A
R2256 T3665 T3664 punct /,TV
R2257 T3666 T3662 punct ;,4A
R2258 T3667 T3662 compound Figure,4A
R2259 T3668 T3662 punct ", ",4A
R2260 T3669 T3662 amod left,4A
R2261 T3670 T3662 punct ),4A
R2262 T3671 T3660 prep in,volume
R2263 T3672 T3673 det the,mice
R2264 T3673 T3671 pobj mice,in
R2265 T3674 T3675 nummod 12,month
R2266 T3675 T3677 npadvmod month,old
R2267 T3676 T3675 punct -,month
R2268 T3677 T3673 amod old,mice
R2269 T3678 T3677 punct -,old
R2270 T3679 T3673 nmod Sam68,mice
R2271 T3680 T3679 punct +,Sam68
R2272 T3681 T3679 punct /,Sam68
R2273 T3682 T3679 punct +,Sam68
R2274 T3683 T3684 punct (,bars
R2275 T3684 T3673 parataxis bars,mice
R2276 T3685 T3684 amod hatched,bars
R2277 T3686 T3684 punct ),bars
R2278 T3687 T3660 prep compared,volume
R2279 T3688 T3687 prep with,compared
R2280 T3689 T3690 nummod 4,month
R2281 T3690 T3692 npadvmod month,old
R2282 T3691 T3690 punct -,month
R2283 T3692 T3694 amod old,mice
R2284 T3693 T3692 punct -,old
R2285 T3694 T3688 pobj mice,with
R2286 T3695 T3694 prep of,mice
R2287 T3696 T3697 det both,genotypes
R2288 T3697 T3695 pobj genotypes,of
R2289 T3698 T3699 punct (,bars
R2290 T3699 T3694 parataxis bars,mice
R2291 T3700 T3699 amod solid,bars
R2292 T3701 T3699 punct ),bars
R2293 T3702 T3694 cc and,mice
R2294 T3703 T3704 nummod 12,month
R2295 T3704 T3706 npadvmod month,old
R2296 T3705 T3704 punct -,month
R2297 T3706 T3708 amod old,mice
R2298 T3707 T3706 punct -,old
R2299 T3708 T3694 conj mice,mice
R2300 T3709 T3708 nmod Sam68,mice
R2301 T3710 T3709 punct −,Sam68
R2302 T3711 T3709 punct /,Sam68
R2303 T3712 T3709 punct −,Sam68
R2304 T3713 T3714 punct (,bars
R2305 T3714 T3708 parataxis bars,mice
R2306 T3715 T3714 amod stippled,bars
R2307 T3716 T3714 punct ),bars
R2308 T3717 T3643 punct .,confirmed
R2309 T3719 T3720 nsubjpass This,associated
R2310 T3721 T3720 auxpass was,associated
R2311 T3722 T3720 prep with,associated
R2312 T3723 T3724 det a,increase
R2313 T3724 T3722 pobj increase,with
R2314 T3725 T3724 amod significant,increase
R2315 T3726 T3727 punct (,denoted
R2316 T3727 T3724 parataxis denoted,increase
R2317 T3728 T3729 nsubj p,0.01
R2318 T3729 T3727 ccomp 0.01,denoted
R2319 T3730 T3729 punct <,0.01
R2320 T3731 T3727 punct ;,denoted
R2321 T3732 T3727 agent by,denoted
R2322 T3733 T3734 det the,asterisks
R2323 T3734 T3732 pobj asterisks,by
R2324 T3735 T3727 punct ),denoted
R2325 T3736 T3724 prep in,increase
R2326 T3737 T3738 det the,index
R2327 T3738 T3736 pobj index,in
R2328 T3739 T3738 compound structure,index
R2329 T3740 T3738 compound model,index
R2330 T3741 T3738 punct ", ",index
R2331 T3742 T3743 dep which,measures
R2332 T3743 T3738 relcl measures,index
R2333 T3744 T3745 det the,ratio
R2334 T3745 T3743 dobj ratio,measures
R2335 T3746 T3745 prep of,ratio
R2336 T3747 T3748 npadvmod plate,like
R2337 T3748 T3746 pobj like,of
R2338 T3749 T3748 punct -,like
R2339 T3750 T3748 prep to,like
R2340 T3751 T3752 npadvmod rod,like
R2341 T3752 T3750 pobj like,to
R2342 T3753 T3752 punct -,like
R2343 T3754 T3748 appos structures,like
R2344 T3755 T3756 punct (,4A
R2345 T3756 T3743 parataxis 4A,measures
R2346 T3757 T3756 compound Figure,4A
R2347 T3758 T3756 punct ", ",4A
R2348 T3759 T3756 appos right,4A
R2349 T3760 T3756 punct ),4A
R2350 T3761 T3720 punct .,associated
R2351 T3763 T3764 det A,increase
R2352 T3764 T3766 nsubj increase,was
R2353 T3765 T3764 amod quantifiable,increase
R2354 T3767 T3764 prep in,increase
R2355 T3768 T3769 det the,separation
R2356 T3769 T3767 pobj separation,in
R2357 T3770 T3769 nmod mean,separation
R2358 T3771 T3769 amod trabecular,separation
R2359 T3772 T3769 punct (,separation
R2360 T3773 T3769 appos Tb.Sp,separation
R2361 T3774 T3769 punct ),separation
R2362 T3775 T3776 punct (,data
R2363 T3776 T3769 parataxis data,separation
R2364 T3777 T3776 amod unpublished,data
R2365 T3778 T3776 punct ),data
R2366 T3779 T3764 prep in,increase
R2367 T3780 T3781 nummod 12,month
R2368 T3781 T3783 npadvmod month,old
R2369 T3782 T3781 punct -,month
R2370 T3783 T3785 amod old,mice
R2371 T3784 T3783 punct -,old
R2372 T3785 T3779 pobj mice,in
R2373 T3786 T3785 nmod Sam68,mice
R2374 T3787 T3786 punct +,Sam68
R2375 T3788 T3786 punct /,Sam68
R2376 T3789 T3786 punct +,Sam68
R2377 T3790 T3766 prep due,was
R2378 T3791 T3790 pcomp to,due
R2379 T3792 T3793 det an,increase
R2380 T3793 T3790 pobj increase,due
R2381 T3794 T3793 prep in,increase
R2382 T3795 T3796 det the,percentage
R2383 T3796 T3794 pobj percentage,in
R2384 T3797 T3796 prep of,percentage
R2385 T3798 T3797 pobj spaces,of
R2386 T3799 T3798 acl falling,spaces
R2387 T3800 T3799 prep in,falling
R2388 T3801 T3802 det the,range
R2389 T3802 T3800 pobj range,in
R2390 T3803 T3802 prep of,range
R2391 T3804 T3805 quantmod 350,700
R2392 T3805 T3807 nummod 700,μm
R2393 T3806 T3805 quantmod to,700
R2394 T3807 T3803 pobj μm,of
R2395 T3808 T3809 punct (,4B
R2396 T3809 T3766 parataxis 4B,was
R2397 T3810 T3809 compound Figure,4B
R2398 T3811 T3809 punct ", ",4B
R2399 T3812 T3813 amod red,line
R2400 T3813 T3809 appos line,4B
R2401 T3814 T3813 amod hatched,line
R2402 T3815 T3809 punct ),4B
R2403 T3816 T3766 punct .,was
R2404 T3818 T3819 amod Trabecular,thickness
R2405 T3819 T3820 nsubj thickness,remained
R2406 T3821 T3820 acomp constant,remained
R2407 T3822 T3820 prep among,remained
R2408 T3823 T3824 det the,groups
R2409 T3824 T3822 pobj groups,among
R2410 T3825 T3824 amod different,groups
R2411 T3826 T3824 prep of,groups
R2412 T3827 T3826 pobj mice,of
R2413 T3828 T3829 punct (,data
R2414 T3829 T3820 meta data,remained
R2415 T3830 T3829 amod unpublished,data
R2416 T3831 T3829 punct ),data
R2417 T3832 T3820 punct .,remained
R2418 T3834 T3835 prep In,meant
R2419 T3836 T3834 pobj effect,In
R2420 T3837 T3835 punct ", ",meant
R2421 T3838 T3835 nsubj this,meant
R2422 T3839 T3840 mark that,were
R2423 T3840 T3835 ccomp were,meant
R2424 T3841 T3840 expl there,were
R2425 T3842 T3843 amod fewer,trabeculae
R2426 T3843 T3840 attr trabeculae,were
R2427 T3844 T3843 punct ", ",trabeculae
R2428 T3845 T3846 advmod rather,than
R2429 T3846 T3843 cc than,trabeculae
R2430 T3847 T3848 amod equivalent,numbers
R2431 T3848 T3843 conj numbers,trabeculae
R2432 T3849 T3848 prep of,numbers
R2433 T3850 T3851 amod thin,trabeculae
R2434 T3851 T3849 pobj trabeculae,of
R2435 T3852 T3843 punct ", ",trabeculae
R2436 T3853 T3843 prep in,trabeculae
R2437 T3854 T3855 det the,mice
R2438 T3855 T3853 pobj mice,in
R2439 T3856 T3857 nummod 12,month
R2440 T3857 T3859 npadvmod month,old
R2441 T3858 T3857 punct -,month
R2442 T3859 T3855 amod old,mice
R2443 T3860 T3859 punct -,old
R2444 T3861 T3855 nmod Sam68,mice
R2445 T3862 T3861 punct +,Sam68
R2446 T3863 T3861 punct /,Sam68
R2447 T3864 T3861 punct +,Sam68
R2448 T3865 T3843 prep compared,trabeculae
R2449 T3866 T3865 prep with,compared
R2450 T3867 T3866 pobj any,with
R2451 T3868 T3867 prep of,any
R2452 T3869 T3870 det the,groups
R2453 T3870 T3868 pobj groups,of
R2454 T3871 T3870 amod other,groups
R2455 T3872 T3870 prep of,groups
R2456 T3873 T3872 pobj mice,of
R2457 T3874 T3835 punct .,meant
R2458 T4163 T4164 compound Bone,Remodeling
R2459 T4164 T4165 nsubjpass Remodeling,Preserved
R2460 T4166 T4165 auxpass Is,Preserved
R2461 T4167 T4165 prep in,Preserved
R2462 T4168 T4169 amod Aged,Mice
R2463 T4169 T4167 pobj Mice,in
R2464 T4170 T4169 nmod Sam68,Mice
R2465 T4171 T4170 punct −,Sam68
R2466 T4172 T4170 punct /,Sam68
R2467 T4173 T4170 punct −,Sam68
R2468 T4175 T4176 aux To,define
R2469 T4176 T4178 advcl define,prepared
R2470 T4177 T4176 advmod further,define
R2471 T4179 T4180 det the,mechanisms
R2472 T4180 T4176 dobj mechanisms,define
R2473 T4181 T4180 acl involved,mechanisms
R2474 T4182 T4181 prep in,involved
R2475 T4183 T4184 det the,preservation
R2476 T4184 T4182 pobj preservation,in
R2477 T4185 T4184 prep of,preservation
R2478 T4186 T4187 compound bone,mass
R2479 T4187 T4185 pobj mass,of
R2480 T4188 T4184 prep in,preservation
R2481 T4189 T4190 amod aged,mice
R2482 T4190 T4188 pobj mice,in
R2483 T4191 T4190 nmod Sam68,mice
R2484 T4192 T4191 punct −,Sam68
R2485 T4193 T4191 punct /,Sam68
R2486 T4194 T4191 punct −,Sam68
R2487 T4195 T4178 punct ", ",prepared
R2488 T4196 T4178 nsubj we,prepared
R2489 T4197 T4178 dobj sections,prepared
R2490 T4198 T4178 prep from,prepared
R2491 T4199 T4200 npadvmod plastic,embedded
R2492 T4200 T4202 amod embedded,femora
R2493 T4201 T4200 punct -,embedded
R2494 T4202 T4198 pobj femora,from
R2495 T4203 T4202 cc and,femora
R2496 T4204 T4202 conj tibia,femora
R2497 T4205 T4206 aux to,evaluate
R2498 T4206 T4178 advcl evaluate,prepared
R2499 T4207 T4208 nmod osteoblast,activity
R2500 T4208 T4206 dobj activity,evaluate
R2501 T4209 T4207 cc and,osteoblast
R2502 T4210 T4207 conj osteoclast,osteoblast
R2503 T4211 T4212 punct (,Figure
R2504 T4212 T4178 parataxis Figure,prepared
R2505 T4213 T4212 nummod 5,Figure
R2506 T4214 T4212 punct ),Figure
R2507 T4215 T4178 punct .,prepared
R2508 T4217 T4218 advmod In,situ
R2509 T4218 T4219 amod situ,staining
R2510 T4219 T4222 nsubjpass staining,used
R2511 T4220 T4219 nmod enzyme,staining
R2512 T4221 T4219 amod histochemical,staining
R2513 T4223 T4219 prep for,staining
R2514 T4224 T4225 nmod alkaline,phosphatase
R2515 T4225 T4226 nmod phosphatase,activity
R2516 T4226 T4223 pobj activity,for
R2517 T4227 T4225 punct (,phosphatase
R2518 T4228 T4225 appos ALP,phosphatase
R2519 T4229 T4230 punct ;,stain
R2520 T4230 T4228 parataxis stain,ALP
R2521 T4231 T4230 amod brown,stain
R2522 T4232 T4230 punct ),stain
R2523 T4233 T4222 auxpass was,used
R2524 T4234 T4222 prep as,used
R2525 T4235 T4236 det a,biomarker
R2526 T4236 T4234 pobj biomarker,as
R2527 T4237 T4236 prep for,biomarker
R2528 T4238 T4237 pobj osteoblasts,for
R2529 T4239 T4238 cc and,osteoblasts
R2530 T4240 T4241 npadvmod tartrate,resistant
R2531 T4241 T4243 amod resistant,activity
R2532 T4242 T4241 punct -,resistant
R2533 T4243 T4238 conj activity,osteoblasts
R2534 T4244 T4243 nmod ALP,activity
R2535 T4245 T4246 punct (,stain
R2536 T4246 T4243 parataxis stain,activity
R2537 T4247 T4248 npadvmod tartrate,resistant
R2538 T4248 T4250 amod resistant,phosphatase
R2539 T4249 T4248 punct -,resistant
R2540 T4250 T4246 dep phosphatase,stain
R2541 T4251 T4250 compound acid,phosphatase
R2542 T4252 T4250 punct [,phosphatase
R2543 T4253 T4250 appos TRAP,phosphatase
R2544 T4254 T4246 punct ],stain
R2545 T4255 T4246 punct ;,stain
R2546 T4256 T4246 amod red,stain
R2547 T4257 T4246 punct ),stain
R2548 T4258 T4222 prep as,used
R2549 T4259 T4260 det a,marker
R2550 T4260 T4258 pobj marker,as
R2551 T4261 T4260 prep for,marker
R2552 T4262 T4261 pobj osteoclasts,for
R2553 T4263 T4222 punct .,used
R2554 T4265 T4266 amod Little,difference
R2555 T4266 T4267 nsubjpass difference,seen
R2556 T4268 T4267 auxpass was,seen
R2557 T4269 T4267 prep between,seen
R2558 T4270 T4271 nmod Sam68,mice
R2559 T4271 T4269 pobj mice,between
R2560 T4272 T4270 punct +,Sam68
R2561 T4273 T4270 punct /,Sam68
R2562 T4274 T4270 punct +,Sam68
R2563 T4275 T4276 punct (,5A
R2564 T4276 T4270 parataxis 5A,Sam68
R2565 T4277 T4276 compound Figure,5A
R2566 T4278 T4276 cc and,5A
R2567 T4279 T4276 conj 5B,5A
R2568 T4280 T4276 punct ),5A
R2569 T4281 T4270 cc and,Sam68
R2570 T4282 T4270 conj Sam68,Sam68
R2571 T4283 T4282 punct −,Sam68
R2572 T4284 T4282 punct /,Sam68
R2573 T4285 T4282 punct −,Sam68
R2574 T4286 T4287 punct (,5C
R2575 T4287 T4282 parataxis 5C,Sam68
R2576 T4288 T4287 compound Figure,5C
R2577 T4289 T4287 cc and,5C
R2578 T4290 T4287 conj 5D,5C
R2579 T4291 T4287 punct ),5C
R2580 T4292 T4267 prep at,seen
R2581 T4293 T4294 nummod 4,months
R2582 T4294 T4292 pobj months,at
R2583 T4295 T4294 prep of,months
R2584 T4296 T4295 pobj age,of
R2585 T4297 T4267 punct .,seen
R2586 T4299 T4300 npadvmod ALP,positive
R2587 T4300 T4305 amod positive,cells
R2588 T4301 T4299 punct -,ALP
R2589 T4302 T4299 cc and,ALP
R2590 T4303 T4299 conj TRAP,ALP
R2591 T4304 T4300 punct -,positive
R2592 T4305 T4306 nsubjpass cells,reduced
R2593 T4307 T4306 auxpass were,reduced
R2594 T4308 T4306 prep in,reduced
R2595 T4309 T4310 det the,mice
R2596 T4310 T4308 pobj mice,in
R2597 T4311 T4312 nummod 12,month
R2598 T4312 T4314 npadvmod month,old
R2599 T4313 T4312 punct -,month
R2600 T4314 T4310 amod old,mice
R2601 T4315 T4314 punct -,old
R2602 T4316 T4310 nmod Sam68,mice
R2603 T4317 T4316 punct +,Sam68
R2604 T4318 T4316 punct /,Sam68
R2605 T4319 T4316 punct +,Sam68
R2606 T4320 T4321 punct (,5E
R2607 T4321 T4306 parataxis 5E,reduced
R2608 T4322 T4321 compound Figure,5E
R2609 T4323 T4321 cc and,5E
R2610 T4324 T4321 conj 5F,5E
R2611 T4325 T4321 punct ),5E
R2612 T4326 T4306 cc and,reduced
R2613 T4327 T4306 conj remained,reduced
R2614 T4328 T4327 acomp unchanged,remained
R2615 T4329 T4327 prep in,remained
R2616 T4330 T4331 nummod 12,month
R2617 T4331 T4333 npadvmod month,old
R2618 T4332 T4331 punct -,month
R2619 T4333 T4335 amod old,mice
R2620 T4334 T4333 punct -,old
R2621 T4335 T4329 pobj mice,in
R2622 T4336 T4335 nmod Sam68,mice
R2623 T4337 T4336 punct −,Sam68
R2624 T4338 T4336 punct /,Sam68
R2625 T4339 T4336 punct −,Sam68
R2626 T4340 T4341 punct (,5G
R2627 T4341 T4327 parataxis 5G,remained
R2628 T4342 T4341 compound Figure,5G
R2629 T4343 T4341 cc and,5G
R2630 T4344 T4341 conj 5H,5G
R2631 T4345 T4341 punct ),5G
R2632 T4346 T4306 punct .,reduced
R2633 T4348 T4349 det The,reduction
R2634 T4349 T4350 nsubj reduction,argued
R2635 T4351 T4349 prep in,reduction
R2636 T4352 T4353 preconj both,osteoblast
R2637 T4353 T4354 nmod osteoblast,activity
R2638 T4354 T4351 pobj activity,in
R2639 T4355 T4353 cc and,osteoblast
R2640 T4356 T4353 conj osteoclast,osteoblast
R2641 T4357 T4349 prep in,reduction
R2642 T4358 T4359 det the,mice
R2643 T4359 T4357 pobj mice,in
R2644 T4360 T4361 nummod 12,month
R2645 T4361 T4363 npadvmod month,old
R2646 T4362 T4361 punct -,month
R2647 T4363 T4359 amod old,mice
R2648 T4364 T4363 punct -,old
R2649 T4365 T4359 nmod Sam68,mice
R2650 T4366 T4365 punct +,Sam68
R2651 T4367 T4365 punct /,Sam68
R2652 T4368 T4365 punct +,Sam68
R2653 T4369 T4350 prep against,argued
R2654 T4370 T4371 nsubjpass bone,lost
R2655 T4371 T4369 pcomp lost,against
R2656 T4372 T4371 auxpass being,lost
R2657 T4373 T4374 advmod primarily,due
R2658 T4374 T4371 prep due,lost
R2659 T4375 T4374 pcomp to,due
R2660 T4376 T4377 det a,increase
R2661 T4377 T4374 pobj increase,due
R2662 T4378 T4377 amod relative,increase
R2663 T4379 T4377 prep in,increase
R2664 T4380 T4379 pobj osteoclast,in
R2665 T4381 T4380 prep over,osteoclast
R2666 T4382 T4381 pobj osteoblast,over
R2667 T4383 T4380 appos activity,osteoclast
R2668 T4384 T4371 punct ", ",lost
R2669 T4385 T4386 mark as,seen
R2670 T4386 T4371 advcl seen,lost
R2671 T4387 T4386 prep in,seen
R2672 T4388 T4389 amod high,turnover
R2673 T4389 T4390 compound turnover,disease
R2674 T4390 T4387 pobj disease,in
R2675 T4391 T4392 punct [,49
R2676 T4392 T4350 parataxis 49,argued
R2677 T4393 T4392 punct ],49
R2678 T4394 T4350 punct .,argued
R2679 T4396 T4397 amod Histomorphometric,analyses
R2680 T4397 T4398 nsubj analyses,corroborated
R2681 T4399 T4400 punct [,50
R2682 T4400 T4397 parataxis 50,analyses
R2683 T4401 T4400 punct ],50
R2684 T4402 T4397 prep of,analyses
R2685 T4403 T4404 det the,bones
R2686 T4404 T4402 pobj bones,of
R2687 T4405 T4404 amod long,bones
R2688 T4406 T4407 punct (,Table
R2689 T4407 T4397 parataxis Table,analyses
R2690 T4408 T4407 nummod 3,Table
R2691 T4409 T4407 punct ),Table
R2692 T4410 T4411 det the,evidence
R2693 T4411 T4398 dobj evidence,corroborated
R2694 T4412 T4411 amod radiologic,evidence
R2695 T4413 T4411 prep of,evidence
R2696 T4414 T4415 npadvmod age,related
R2697 T4415 T4417 amod related,loss
R2698 T4416 T4415 punct -,related
R2699 T4417 T4413 pobj loss,of
R2700 T4418 T4417 compound bone,loss
R2701 T4419 T4398 punct .,corroborated
R2702 T4421 T4422 compound Bone,volume
R2703 T4422 T4423 nsubjpass volume,reduced
R2704 T4424 T4422 punct (,volume
R2705 T4425 T4426 compound BV,TV
R2706 T4426 T4422 appos TV,volume
R2707 T4427 T4426 punct /,TV
R2708 T4428 T4422 punct ),volume
R2709 T4429 T4422 punct ", ",volume
R2710 T4430 T4431 advmod newly,formed
R2711 T4431 T4432 amod formed,osteoid
R2712 T4432 T4422 conj osteoid,volume
R2713 T4433 T4432 punct (,osteoid
R2714 T4434 T4435 compound OV,TV
R2715 T4435 T4432 appos TV,osteoid
R2716 T4436 T4435 punct /,TV
R2717 T4437 T4432 punct ),osteoid
R2718 T4438 T4432 punct ", ",osteoid
R2719 T4439 T4432 cc and,osteoid
R2720 T4440 T4441 compound mineral,rate
R2721 T4441 T4432 conj rate,osteoid
R2722 T4442 T4441 compound apposition,rate
R2723 T4443 T4441 punct (,rate
R2724 T4444 T4441 appos MAR,rate
R2725 T4445 T4423 punct ),reduced
R2726 T4446 T4423 auxpass were,reduced
R2727 T4447 T4423 dep all,reduced
R2728 T4448 T4423 advmod significantly,reduced
R2729 T4449 T4423 prep in,reduced
R2730 T4450 T4451 nummod 12,month
R2731 T4451 T4453 npadvmod month,old
R2732 T4452 T4451 punct -,month
R2733 T4453 T4455 amod old,mice
R2734 T4454 T4453 punct -,old
R2735 T4455 T4449 pobj mice,in
R2736 T4456 T4455 nmod Sam68,mice
R2737 T4457 T4456 punct +,Sam68
R2738 T4458 T4456 punct /,Sam68
R2739 T4459 T4456 punct +,Sam68
R2740 T4460 T4423 prep compared,reduced
R2741 T4461 T4460 prep with,compared
R2742 T4462 T4463 nummod 4,month
R2743 T4463 T4465 npadvmod month,old
R2744 T4464 T4463 punct -,month
R2745 T4465 T4467 amod old,mice
R2746 T4466 T4465 punct -,old
R2747 T4467 T4461 pobj mice,with
R2748 T4468 T4467 nmod Sam68,mice
R2749 T4469 T4468 punct +,Sam68
R2750 T4470 T4468 punct /,Sam68
R2751 T4471 T4468 punct +,Sam68
R2752 T4472 T4423 punct .,reduced
R2753 T4474 T4475 det These,reductions
R2754 T4475 T4476 nsubjpass reductions,associated
R2755 T4477 T4476 auxpass were,associated
R2756 T4478 T4476 prep with,associated
R2757 T4479 T4480 det a,increase
R2758 T4480 T4478 pobj increase,with
R2759 T4481 T4480 amod significant,increase
R2760 T4482 T4480 prep in,increase
R2761 T4483 T4484 compound marrow,fat
R2762 T4484 T4482 pobj fat,in
R2763 T4485 T4486 punct (,TV
R2764 T4486 T4480 parataxis TV,increase
R2765 T4487 T4486 compound FV,TV
R2766 T4488 T4486 punct /,TV
R2767 T4489 T4486 punct ),TV
R2768 T4490 T4480 cc and,increase
R2769 T4491 T4492 amod decreased,numbers
R2770 T4492 T4480 conj numbers,increase
R2771 T4493 T4492 prep of,numbers
R2772 T4494 T4493 pobj osteoblasts,of
R2773 T4495 T4496 punct (,TV
R2774 T4496 T4494 parataxis TV,osteoblasts
R2775 T4497 T4496 compound nOB,TV
R2776 T4498 T4496 punct /,TV
R2777 T4499 T4496 punct ),TV
R2778 T4500 T4494 cc and,osteoblasts
R2779 T4501 T4494 conj osteoclasts,osteoblasts
R2780 T4502 T4503 punct (,TV
R2781 T4503 T4501 parataxis TV,osteoclasts
R2782 T4504 T4503 compound nOC,TV
R2783 T4505 T4503 punct /,TV
R2784 T4506 T4503 punct ),TV
R2785 T4507 T4492 prep per,numbers
R2786 T4508 T4509 compound tissue,volume
R2787 T4509 T4507 pobj volume,per
R2788 T4510 T4511 punct (,Table
R2789 T4511 T4476 parataxis Table,associated
R2790 T4512 T4511 nummod 3,Table
R2791 T4513 T4511 punct ),Table
R2792 T4514 T4476 punct .,associated
R2793 T4516 T4517 det These,results
R2794 T4517 T4518 nsubj results,were
R2795 T4519 T4518 prep in,were
R2796 T4520 T4519 pobj contrast,in
R2797 T4521 T4520 prep to,contrast
R2798 T4522 T4521 pobj those,to
R2799 T4523 T4522 prep of,those
R2800 T4524 T4525 det the,mice
R2801 T4525 T4523 pobj mice,of
R2802 T4526 T4527 nummod 12,month
R2803 T4527 T4529 npadvmod month,old
R2804 T4528 T4527 punct -,month
R2805 T4529 T4525 amod old,mice
R2806 T4530 T4529 punct -,old
R2807 T4531 T4525 nmod Sam68,mice
R2808 T4532 T4531 punct −,Sam68
R2809 T4533 T4531 punct /,Sam68
R2810 T4534 T4531 punct −,Sam68
R2811 T4535 T4536 prep in,resembled
R2812 T4536 T4522 relcl resembled,those
R2813 T4537 T4535 pobj which,in
R2814 T4538 T4539 det all,parameters
R2815 T4539 T4536 nsubj parameters,resembled
R2816 T4540 T4539 amod histomorphometric,parameters
R2817 T4541 T4539 punct ", ",parameters
R2818 T4542 T4539 prep including,parameters
R2819 T4543 T4544 compound marrow,fat
R2820 T4544 T4542 pobj fat,including
R2821 T4545 T4536 punct ", ",resembled
R2822 T4546 T4536 dobj those,resembled
R2823 T4547 T4546 prep of,those
R2824 T4548 T4549 amod young,mice
R2825 T4549 T4547 pobj mice,of
R2826 T4550 T4549 prep of,mice
R2827 T4551 T4552 preconj either,genotype
R2828 T4552 T4550 pobj genotype,of
R2829 T4553 T4554 punct (,Table
R2830 T4554 T4536 parataxis Table,resembled
R2831 T4555 T4554 nummod 3,Table
R2832 T4556 T4554 punct ),Table
R2833 T4557 T4518 punct .,were
R2834 T4559 T4560 advmod When,expressed
R2835 T4560 T4563 advcl expressed,were
R2836 T4561 T4560 nsubjpass cells,expressed
R2837 T4562 T4560 auxpass were,expressed
R2838 T4564 T4560 prep as,expressed
R2839 T4565 T4566 det a,function
R2840 T4566 T4564 pobj function,as
R2841 T4567 T4566 prep of,function
R2842 T4568 T4569 det the,perimeter
R2843 T4569 T4567 pobj perimeter,of
R2844 T4570 T4569 compound bone,perimeter
R2845 T4571 T4572 punct (,BP
R2846 T4572 T4560 parataxis BP,expressed
R2847 T4573 T4572 compound nOB,BP
R2848 T4574 T4572 punct /,BP
R2849 T4575 T4572 punct ", ",BP
R2850 T4576 T4577 compound nOC,BP
R2851 T4577 T4572 appos BP,BP
R2852 T4578 T4577 punct /,BP
R2853 T4579 T4572 punct ),BP
R2854 T4580 T4563 punct ", ",were
R2855 T4581 T4563 expl there,were
R2856 T4582 T4583 det no,differences
R2857 T4583 T4563 attr differences,were
R2858 T4584 T4583 amod statistical,differences
R2859 T4585 T4563 cc and,were
R2860 T4586 T4587 det the,ratio
R2861 T4587 T4588 nsubj ratio,was
R2862 T4588 T4563 conj was,were
R2863 T4589 T4587 prep of,ratio
R2864 T4590 T4589 pobj osteoblasts,of
R2865 T4591 T4590 prep to,osteoblasts
R2866 T4592 T4591 pobj osteoclasts,to
R2867 T4593 T4588 acomp similar,was
R2868 T4594 T4588 prep in,was
R2869 T4595 T4596 det all,groups
R2870 T4596 T4594 pobj groups,in
R2871 T4597 T4596 prep of,groups
R2872 T4598 T4597 pobj mice,of
R2873 T4599 T4600 punct (,Table
R2874 T4600 T4588 parataxis Table,was
R2875 T4601 T4600 nummod 3,Table
R2876 T4602 T4600 punct ),Table
R2877 T4603 T4563 punct .,were
R2878 T4605 T4606 amod Circulating,levels
R2879 T4606 T4607 nsubj levels,showed
R2880 T4608 T4606 prep of,levels
R2881 T4609 T4610 compound serum,telopeptide
R2882 T4610 T4608 pobj telopeptide,of
R2883 T4611 T4610 compound C,telopeptide
R2884 T4612 T4610 punct -,telopeptide
R2885 T4613 T4614 punct (,Diagnostics
R2886 T4614 T4610 parataxis Diagnostics,telopeptide
R2887 T4615 T4614 dep sCTX,Diagnostics
R2888 T4616 T4614 punct ;,Diagnostics
R2889 T4617 T4614 compound Roche,Diagnostics
R2890 T4618 T4614 punct ", ",Diagnostics
R2891 T4619 T4614 npadvmod Mannheim,Diagnostics
R2892 T4620 T4614 punct ", ",Diagnostics
R2893 T4621 T4614 npadvmod Germany,Diagnostics
R2894 T4622 T4614 punct ),Diagnostics
R2895 T4623 T4610 cc and,telopeptide
R2896 T4624 T4610 conj ALP,telopeptide
R2897 T4625 T4626 amod little,difference
R2898 T4626 T4607 dobj difference,showed
R2899 T4627 T4607 prep among,showed
R2900 T4628 T4629 det the,groups
R2901 T4629 T4627 pobj groups,among
R2902 T4630 T4607 punct ", ",showed
R2903 T4631 T4607 prep except,showed
R2904 T4632 T4631 prep for,except
R2905 T4633 T4634 det a,decrease
R2906 T4634 T4632 pobj decrease,for
R2907 T4635 T4634 amod small,decrease
R2908 T4636 T4634 prep in,decrease
R2909 T4637 T4638 nummod 12,month
R2910 T4638 T4640 npadvmod month,old
R2911 T4639 T4638 punct -,month
R2912 T4640 T4642 amod old,mice
R2913 T4641 T4640 punct -,old
R2914 T4642 T4636 pobj mice,in
R2915 T4643 T4642 nmod Sam68,mice
R2916 T4644 T4643 punct −,Sam68
R2917 T4645 T4643 punct /,Sam68
R2918 T4646 T4643 punct −,Sam68
R2919 T4647 T4642 cc and,mice
R2920 T4648 T4649 punct (,S3
R2921 T4649 T4642 conj S3,mice
R2922 T4650 T4649 compound Dataset,S3
R2923 T4651 T4607 punct ),showed
R2924 T4652 T4607 punct .,showed
R2925 T4654 T4655 compound Serum,estrogen
R2926 T4655 T4656 nsubjpass estrogen,decreased
R2927 T4657 T4656 auxpass was,decreased
R2928 T4658 T4656 advmod significantly,decreased
R2929 T4659 T4656 prep in,decreased
R2930 T4660 T4661 det all,mice
R2931 T4661 T4659 pobj mice,in
R2932 T4662 T4663 nummod 12,month
R2933 T4663 T4665 npadvmod month,old
R2934 T4664 T4663 punct -,month
R2935 T4665 T4661 amod old,mice
R2936 T4666 T4665 punct -,old
R2937 T4667 T4656 advmod regardless,decreased
R2938 T4668 T4667 prep of,regardless
R2939 T4669 T4668 pobj genotype,of
R2940 T4670 T4656 prep with,decreased
R2941 T4671 T4672 det a,increase
R2942 T4672 T4670 pobj increase,with
R2943 T4673 T4672 amod reciprocal,increase
R2944 T4674 T4672 prep in,increase
R2945 T4675 T4676 nmod interleukin,levels
R2946 T4676 T4674 pobj levels,in
R2947 T4677 T4675 punct -,interleukin
R2948 T4678 T4675 nummod 6,interleukin
R2949 T4679 T4680 punct (,S3
R2950 T4680 T4656 parataxis S3,decreased
R2951 T4681 T4680 compound Dataset,S3
R2952 T4682 T4680 punct ),S3
R2953 T4683 T4656 punct .,decreased
R2954 T4685 T4686 prep Given,measured
R2955 T4687 T4688 det the,involvement
R2956 T4688 T4685 pobj involvement,Given
R2957 T4689 T4688 prep of,involvement
R2958 T4690 T4689 pobj leptin,of
R2959 T4691 T4688 prep in,involvement
R2960 T4692 T4691 pcomp regulating,in
R2961 T4693 T4694 nmod body,mass
R2962 T4694 T4692 dobj mass,regulating
R2963 T4695 T4693 cc and,body
R2964 T4696 T4693 conj bone,body
R2965 T4697 T4686 punct ", ",measured
R2966 T4698 T4699 compound serum,leptin
R2967 T4699 T4686 nsubjpass leptin,measured
R2968 T4700 T4686 auxpass was,measured
R2969 T4701 T4686 prep in,measured
R2970 T4702 T4703 nmod Sam68,mice
R2971 T4703 T4701 pobj mice,in
R2972 T4704 T4702 punct +,Sam68
R2973 T4705 T4702 punct /,Sam68
R2974 T4706 T4702 punct +,Sam68
R2975 T4707 T4702 cc and,Sam68
R2976 T4708 T4702 conj Sam68,Sam68
R2977 T4709 T4708 punct −,Sam68
R2978 T4710 T4708 punct /,Sam68
R2979 T4711 T4708 punct −,Sam68
R2980 T4712 T4686 punct .,measured
R2981 T4714 T4715 nummod Twelve,month
R2982 T4715 T4717 npadvmod month,old
R2983 T4716 T4715 punct -,month
R2984 T4717 T4719 amod old,mice
R2985 T4718 T4717 punct -,old
R2986 T4719 T4724 nsubj mice,had
R2987 T4720 T4719 nmod Sam68,mice
R2988 T4721 T4720 punct −,Sam68
R2989 T4722 T4720 punct /,Sam68
R2990 T4723 T4720 punct −,Sam68
R2991 T4725 T4726 advmod significantly,lower
R2992 T4726 T4727 amod lower,levels
R2993 T4727 T4724 dobj levels,had
R2994 T4728 T4727 prep than,levels
R2995 T4729 T4730 amod young,mice
R2996 T4730 T4728 pobj mice,than
R2997 T4731 T4729 cc and,young
R2998 T4732 T4729 conj old,young
R2999 T4733 T4730 nmod Sam68,mice
R3000 T4734 T4733 punct +,Sam68
R3001 T4735 T4733 punct /,Sam68
R3002 T4736 T4733 punct +,Sam68
R3003 T4737 T4730 cc and,mice
R3004 T4738 T4739 amod young,mice
R3005 T4739 T4730 conj mice,mice
R3006 T4740 T4739 nmod Sam68,mice
R3007 T4741 T4740 punct −,Sam68
R3008 T4742 T4740 punct /,Sam68
R3009 T4743 T4740 punct −,Sam68
R3010 T4744 T4745 punct (,S3
R3011 T4745 T4724 parataxis S3,had
R3012 T4746 T4745 compound Dataset,S3
R3013 T4747 T4745 punct ),S3
R3014 T4748 T4724 punct .,had
R3015 T4750 T4751 advcl Taken,suggested
R3016 T4752 T4750 advmod together,Taken
R3017 T4753 T4751 punct ", ",suggested
R3018 T4754 T4755 det these,observations
R3019 T4755 T4751 nsubj observations,suggested
R3020 T4756 T4757 mark that,was
R3021 T4757 T4751 ccomp was,suggested
R3022 T4758 T4757 nsubj preservation,was
R3023 T4759 T4758 prep of,preservation
R3024 T4760 T4761 compound bone,mass
R3025 T4761 T4759 pobj mass,of
R3026 T4762 T4758 prep in,preservation
R3027 T4763 T4764 det the,mice
R3028 T4764 T4762 pobj mice,in
R3029 T4765 T4764 nmod Sam68,mice
R3030 T4766 T4765 punct −,Sam68
R3031 T4767 T4765 punct /,Sam68
R3032 T4768 T4765 punct −,Sam68
R3033 T4769 T4757 neg not,was
R3034 T4770 T4757 prep due,was
R3035 T4771 T4770 advmod primarily,due
R3036 T4772 T4770 pcomp to,due
R3037 T4773 T4774 amod altered,status
R3038 T4774 T4770 pobj status,due
R3039 T4775 T4774 compound estrogen,status
R3040 T4776 T4757 cc but,was
R3041 T4777 T4778 aux could,influenced
R3042 T4778 T4757 conj influenced,was
R3043 T4779 T4778 aux have,influenced
R3044 T4780 T4778 auxpass been,influenced
R3045 T4781 T4778 agent by,influenced
R3046 T4782 T4781 pobj differences,by
R3047 T4783 T4782 prep in,differences
R3048 T4784 T4785 amod circulating,levels
R3049 T4785 T4783 pobj levels,in
R3050 T4786 T4785 compound leptin,levels
R3051 T4787 T4751 punct .,suggested
R3052 T4956 T4957 nmod Osteoblast,Activity
R3053 T4957 T4963 nsubjpass Activity,Altered
R3054 T4958 T4956 punct ", ",Osteoblast
R3055 T4959 T4956 cc but,Osteoblast
R3056 T4960 T4959 neg Not,but
R3057 T4961 T4956 conj Osteoclast,Osteoblast
R3058 T4962 T4957 punct ", ",Activity
R3059 T4964 T4963 auxpass Is,Altered
R3060 T4965 T4963 prep in,Altered
R3061 T4966 T4967 nmod Sam68,Mice
R3062 T4967 T4965 pobj Mice,in
R3063 T4968 T4966 punct −,Sam68
R3064 T4969 T4966 punct /,Sam68
R3065 T4970 T4966 punct −,Sam68
R3066 T4971 T4972 advmod Ex,Vivo
R3067 T4972 T4963 advmod Vivo,Altered
R3068 T4974 T4975 nsubj Maintenance,is
R3069 T4976 T4974 prep of,Maintenance
R3070 T4977 T4978 compound bone,mass
R3071 T4978 T4976 pobj mass,of
R3072 T4979 T4974 prep during,Maintenance
R3073 T4980 T4981 det the,cycle
R3074 T4981 T4979 pobj cycle,during
R3075 T4982 T4981 amod adult,cycle
R3076 T4983 T4981 compound remodeling,cycle
R3077 T4984 T4975 acomp dependent,is
R3078 T4985 T4984 prep on,dependent
R3079 T4986 T4987 det the,coupling
R3080 T4987 T4985 pobj coupling,on
R3081 T4988 T4987 prep of,coupling
R3082 T4989 T4988 pobj osteoblast,of
R3083 T4990 T4987 prep to,coupling
R3084 T4991 T4990 pobj osteoclast,to
R3085 T4992 T4987 appos activity,coupling
R3086 T4993 T4975 punct ", ",is
R3087 T4994 T4995 amod such,is
R3088 T4995 T4975 advcl is,is
R3089 T4996 T4995 mark that,is
R3090 T4997 T4995 expl there,is
R3091 T4998 T4999 det no,gain
R3092 T4999 T4995 attr gain,is
R3093 T5000 T4999 amod net,gain
R3094 T5001 T4999 cc or,gain
R3095 T5002 T4999 conj loss,gain
R3096 T5003 T4999 prep of,gain
R3097 T5004 T5003 pobj bone,of
R3098 T5005 T5006 punct [,49
R3099 T5006 T4975 parataxis 49,is
R3100 T5007 T5006 punct ],49
R3101 T5008 T4975 punct .,is
R3102 T5010 T5011 aux To,explore
R3103 T5011 T5013 advcl explore,examined
R3104 T5012 T5011 advmod further,explore
R3105 T5014 T5015 det the,advantage
R3106 T5015 T5011 dobj advantage,explore
R3107 T5016 T5017 npadvmod age,related
R3108 T5017 T5015 amod related,advantage
R3109 T5018 T5017 punct -,related
R3110 T5019 T5015 prep of,advantage
R3111 T5020 T5021 nmod Sam68,mice
R3112 T5021 T5019 pobj mice,of
R3113 T5022 T5020 punct −,Sam68
R3114 T5023 T5020 punct /,Sam68
R3115 T5024 T5020 punct −,Sam68
R3116 T5025 T5011 prep with,explore
R3117 T5026 T5025 pobj respect,with
R3118 T5027 T5026 prep to,respect
R3119 T5028 T5029 compound bone,preservation
R3120 T5029 T5027 pobj preservation,to
R3121 T5030 T5013 punct ", ",examined
R3122 T5031 T5013 nsubj we,examined
R3123 T5032 T5033 det the,activity
R3124 T5033 T5013 dobj activity,examined
R3125 T5034 T5033 amod functional,activity
R3126 T5035 T5033 prep of,activity
R3127 T5036 T5035 pobj Sam68,of
R3128 T5037 T5036 punct −,Sam68
R3129 T5038 T5036 punct /,Sam68
R3130 T5039 T5036 punct −,Sam68
R3131 T5040 T5036 appos osteoblasts,Sam68
R3132 T5041 T5040 cc and,osteoblasts
R3133 T5042 T5040 conj osteoclasts,osteoblasts
R3134 T5043 T5044 advmod ex,vivo
R3135 T5044 T5013 advmod vivo,examined
R3136 T5045 T5046 punct (,6A
R3137 T5046 T5013 parataxis 6A,examined
R3138 T5047 T5046 compound Figure,6A
R3139 T5048 T5046 cc and,6A
R3140 T5049 T5046 conj 6B,6A
R3141 T5050 T5046 punct ),6A
R3142 T5051 T5013 punct .,examined
R3143 T5053 T5054 nsubj Cultures,demonstrated
R3144 T5055 T5053 prep of,Cultures
R3145 T5056 T5057 nmod bone,marrow
R3146 T5057 T5058 nmod marrow,cells
R3147 T5058 T5055 pobj cells,of
R3148 T5059 T5058 amod stromal,cells
R3149 T5060 T5053 acl harvested,Cultures
R3150 T5061 T5060 prep from,harvested
R3151 T5062 T5063 nummod 4,week
R3152 T5063 T5065 npadvmod week,old
R3153 T5064 T5063 punct -,week
R3154 T5065 T5067 amod old,mice
R3155 T5066 T5065 punct -,old
R3156 T5067 T5061 pobj mice,from
R3157 T5068 T5067 nmod Sam68,mice
R3158 T5069 T5068 punct −,Sam68
R3159 T5070 T5068 punct /,Sam68
R3160 T5071 T5068 punct −,Sam68
R3161 T5072 T5060 cc and,harvested
R3162 T5073 T5060 conj maintained,harvested
R3163 T5074 T5073 prep for,maintained
R3164 T5075 T5076 nummod 18,days
R3165 T5076 T5074 pobj days,for
R3166 T5077 T5073 prep in,maintained
R3167 T5078 T5079 compound osteoblast,differentiation
R3168 T5079 T5080 compound differentiation,medium
R3169 T5080 T5077 pobj medium,in
R3170 T5081 T5082 advmod more,intense
R3171 T5082 T5083 amod intense,staining
R3172 T5083 T5054 dobj staining,demonstrated
R3173 T5084 T5083 prep for,staining
R3174 T5085 T5084 pobj ALP,for
R3175 T5086 T5054 prep at,demonstrated
R3176 T5087 T5088 nummod 6,days
R3177 T5088 T5086 pobj days,at
R3178 T5089 T5090 punct (,data
R3179 T5090 T5088 meta data,days
R3180 T5091 T5090 amod unpublished,data
R3181 T5092 T5090 punct ),data
R3182 T5093 T5088 cc and,days
R3183 T5094 T5095 nummod 18,days
R3184 T5095 T5088 conj days,days
R3185 T5096 T5097 punct (,6A
R3186 T5097 T5095 parataxis 6A,days
R3187 T5098 T5097 compound Figure,6A
R3188 T5099 T5097 dep ),6A
R3189 T5100 T5054 dep ", ",demonstrated
R3190 T5101 T5054 cc and,demonstrated
R3191 T5102 T5103 amod more,nodules
R3192 T5103 T5054 conj nodules,demonstrated
R3193 T5104 T5103 amod mineralized,nodules
R3194 T5105 T5103 prep at,nodules
R3195 T5106 T5107 nummod 18,days
R3196 T5107 T5105 pobj days,at
R3197 T5108 T5054 dep ", ",demonstrated
R3198 T5109 T5054 prep than,demonstrated
R3199 T5110 T5111 det the,mice
R3200 T5111 T5109 pobj mice,than
R3201 T5112 T5111 nmod Sam68,mice
R3202 T5113 T5112 punct +,Sam68
R3203 T5114 T5112 punct /,Sam68
R3204 T5115 T5112 punct +,Sam68
R3205 T5116 T5054 dep ", ",demonstrated
R3206 T5117 T5118 advmod even,observed
R3207 T5118 T5054 advcl observed,demonstrated
R3208 T5119 T5118 mark though,observed
R3209 T5120 T5121 amod similar,amounts
R3210 T5121 T5118 nsubjpass amounts,observed
R3211 T5122 T5121 prep of,amounts
R3212 T5123 T5124 nmod fibroblast,units
R3213 T5124 T5122 pobj units,of
R3214 T5125 T5126 npadvmod colony,forming
R3215 T5126 T5124 amod forming,units
R3216 T5127 T5126 punct -,forming
R3217 T5128 T5124 punct (,units
R3218 T5129 T5130 compound CFU,F
R3219 T5130 T5124 appos F,units
R3220 T5131 T5130 punct -,F
R3221 T5132 T5118 punct ),observed
R3222 T5133 T5118 auxpass were,observed
R3223 T5134 T5135 punct (,6A
R3224 T5135 T5118 parataxis 6A,observed
R3225 T5136 T5135 compound Figure,6A
R3226 T5137 T5135 cc and,6A
R3227 T5138 T5139 amod unpublished,data
R3228 T5139 T5135 conj data,6A
R3229 T5140 T5135 punct ),6A
R3230 T5141 T5054 punct .,demonstrated
R3231 T5143 T5144 compound RT,PCR
R3232 T5144 T5146 compound PCR,analysis
R3233 T5145 T5144 punct -,PCR
R3234 T5146 T5147 nsubj analysis,revealed
R3235 T5148 T5146 prep of,analysis
R3236 T5149 T5150 amod molecular,markers
R3237 T5150 T5148 pobj markers,of
R3238 T5151 T5150 prep of,markers
R3239 T5152 T5153 compound osteoblast,differentiation
R3240 T5153 T5151 pobj differentiation,of
R3241 T5154 T5155 punct (,S2
R3242 T5155 T5146 parataxis S2,analysis
R3243 T5156 T5155 compound Dataset,S2
R3244 T5157 T5155 punct ),S2
R3245 T5158 T5159 amod similar,increases
R3246 T5159 T5147 dobj increases,revealed
R3247 T5160 T5147 prep over,revealed
R3248 T5161 T5160 pobj time,over
R3249 T5162 T5147 prep in,revealed
R3250 T5163 T5162 pobj RNA,in
R3251 T5164 T5163 prep from,RNA
R3252 T5165 T5166 nmod Sam68,mice
R3253 T5166 T5164 pobj mice,from
R3254 T5167 T5165 punct +,Sam68
R3255 T5168 T5165 punct /,Sam68
R3256 T5169 T5165 punct +,Sam68
R3257 T5170 T5165 cc and,Sam68
R3258 T5171 T5165 conj Sam68,Sam68
R3259 T5172 T5171 punct −,Sam68
R3260 T5173 T5171 punct /,Sam68
R3261 T5174 T5171 punct −,Sam68
R3262 T5175 T5147 punct ", ",revealed
R3263 T5176 T5177 mark although,appeared
R3264 T5177 T5147 advcl appeared,revealed
R3265 T5178 T5179 nmod type,collagen
R3266 T5179 T5177 nsubj collagen,appeared
R3267 T5180 T5178 nummod I,type
R3268 T5181 T5182 aux to,regulated
R3269 T5182 T5177 xcomp regulated,appeared
R3270 T5183 T5182 auxpass be,regulated
R3271 T5184 T5182 advmod up,regulated
R3272 T5185 T5182 punct -,regulated
R3273 T5186 T5182 prep at,regulated
R3274 T5187 T5188 det both,timepoints
R3275 T5188 T5186 pobj timepoints,at
R3276 T5189 T5182 prep in,regulated
R3277 T5190 T5191 det the,mice
R3278 T5191 T5189 pobj mice,in
R3279 T5192 T5191 nmod Sam68,mice
R3280 T5193 T5192 punct −,Sam68
R3281 T5194 T5192 punct /,Sam68
R3282 T5195 T5192 punct −,Sam68
R3283 T5196 T5147 punct .,revealed
R3284 T5198 T5199 amod Short,term
R3285 T5199 T5201 compound term,cultures
R3286 T5200 T5199 punct -,term
R3287 T5201 T5202 nsubj cultures,stained
R3288 T5203 T5201 prep of,cultures
R3289 T5204 T5205 amod mature,osteoclasts
R3290 T5205 T5203 pobj osteoclasts,of
R3291 T5206 T5205 acl released,osteoclasts
R3292 T5207 T5206 prep from,released
R3293 T5208 T5209 det the,bones
R3294 T5209 T5207 pobj bones,from
R3295 T5210 T5209 amod crushed,bones
R3296 T5211 T5209 amod long,bones
R3297 T5212 T5209 prep of,bones
R3298 T5213 T5214 nmod Sam68,mice
R3299 T5214 T5212 pobj mice,of
R3300 T5215 T5213 punct +,Sam68
R3301 T5216 T5213 punct /,Sam68
R3302 T5217 T5213 punct +,Sam68
R3303 T5218 T5213 cc and,Sam68
R3304 T5219 T5213 conj Sam68,Sam68
R3305 T5220 T5219 punct −,Sam68
R3306 T5221 T5219 punct /,Sam68
R3307 T5222 T5219 punct −,Sam68
R3308 T5223 T5224 advmod equally,well
R3309 T5224 T5202 advmod well,stained
R3310 T5225 T5202 prep for,stained
R3311 T5226 T5225 pobj TRAP,for
R3312 T5227 T5202 cc and,stained
R3313 T5228 T5202 conj excavated,stained
R3314 T5229 T5230 advmod approximately,equal
R3315 T5230 T5231 amod equal,numbers
R3316 T5231 T5228 dobj numbers,excavated
R3317 T5232 T5231 prep of,numbers
R3318 T5233 T5232 pobj pits,of
R3319 T5234 T5233 prep of,pits
R3320 T5235 T5236 amod equal,size
R3321 T5236 T5234 pobj size,of
R3322 T5237 T5228 prep in,excavated
R3323 T5238 T5239 compound dentin,slices
R3324 T5239 T5237 pobj slices,in
R3325 T5240 T5228 punct ", ",excavated
R3326 T5241 T5242 mark as,visualized
R3327 T5242 T5228 advcl visualized,excavated
R3328 T5243 T5242 prep by,visualized
R3329 T5244 T5243 pcomp scanning,by
R3330 T5245 T5246 compound electron,microscopy
R3331 T5246 T5244 dobj microscopy,scanning
R3332 T5247 T5248 punct (,6B
R3333 T5248 T5228 parataxis 6B,excavated
R3334 T5249 T5248 compound Figure,6B
R3335 T5250 T5248 punct ),6B
R3336 T5251 T5202 punct .,stained
R3337 T5253 T5254 det These,findings
R3338 T5254 T5255 nsubj findings,suggest
R3339 T5256 T5257 mark that,be
R3340 T5257 T5255 ccomp be,suggest
R3341 T5258 T5259 det the,osteoclasts
R3342 T5259 T5257 nsubj osteoclasts,be
R3343 T5260 T5257 aux may,be
R3344 T5261 T5257 neg not,be
R3345 T5262 T5263 det the,defect
R3346 T5263 T5257 attr defect,be
R3347 T5264 T5263 amod primary,defect
R3348 T5265 T5263 prep in,defect
R3349 T5266 T5267 nmod Sam68,mice
R3350 T5267 T5265 pobj mice,in
R3351 T5268 T5266 punct −,Sam68
R3352 T5269 T5266 punct /,Sam68
R3353 T5270 T5266 punct −,Sam68
R3354 T5271 T5257 punct ", ",be
R3355 T5272 T5273 mark as,are
R3356 T5273 T5257 advcl are,be
R3357 T5274 T5273 nsubj they,are
R3358 T5275 T5273 prep in,are
R3359 T5276 T5277 nmod Src,mice
R3360 T5277 T5275 pobj mice,in
R3361 T5278 T5277 amod null,mice
R3362 T5279 T5280 punct [,48
R3363 T5280 T5273 parataxis 48,are
R3364 T5281 T5280 punct ],48
R3365 T5282 T5255 punct .,suggest
R3377 T5689 T5690 det The,Absence
R3378 T5690 T5691 nsubj Absence,Prevents
R3379 T5692 T5690 prep of,Absence
R3380 T5693 T5692 pobj Sam68,of
R3381 T5694 T5695 compound Adipocyte,Differentiation
R3382 T5695 T5691 dobj Differentiation,Prevents
R3383 T5696 T5691 cc and,Prevents
R3384 T5697 T5691 conj Promotes,Prevents
R3385 T5698 T5699 compound Osteoblast,Differentiation
R3386 T5699 T5697 dobj Differentiation,Promotes
R3387 T5701 T5702 nsubjpass It,recognized
R3388 T5703 T5702 auxpass is,recognized
R3389 T5704 T5702 advmod well,recognized
R3390 T5705 T5706 mark that,give
R3391 T5706 T5702 advcl give,recognized
R3392 T5707 T5708 nmod bone,marrow
R3393 T5708 T5709 nmod marrow,cells
R3394 T5709 T5706 nsubj cells,give
R3395 T5710 T5709 amod stromal,cells
R3396 T5711 T5706 dobj rise,give
R3397 T5712 T5706 prep to,give
R3398 T5713 T5714 det both,osteoblasts
R3399 T5714 T5712 pobj osteoblasts,to
R340 T1057 T1058 prep During,favored
R3400 T5715 T5714 cc and,osteoblasts
R3401 T5716 T5714 conj adipocytes,osteoblasts
R3402 T5717 T5706 cc and,give
R3403 T5718 T5719 mark that,accompanied
R3404 T5719 T5706 conj accompanied,give
R3405 T5720 T5721 npadvmod age,related
R3406 T5721 T5723 amod related,loss
R3407 T5722 T5721 punct -,related
R3408 T5723 T5719 nsubjpass loss,accompanied
R3409 T5724 T5723 compound bone,loss
R341 T1059 T1060 amod skeletal,development
R3410 T5725 T5719 auxpass is,accompanied
R3411 T5726 T5719 agent by,accompanied
R3412 T5727 T5728 det an,increase
R3413 T5728 T5726 pobj increase,by
R3414 T5729 T5728 prep in,increase
R3415 T5730 T5729 pobj differentiation,in
R3416 T5731 T5719 prep down,accompanied
R3417 T5732 T5733 det the,lineage
R3418 T5733 T5731 pobj lineage,down
R3419 T5734 T5733 compound adipocyte,lineage
R342 T1060 T1057 pobj development,During
R3420 T5735 T5736 punct [,22
R3421 T5736 T5719 parataxis 22,accompanied
R3422 T5737 T5736 punct ],22
R3423 T5738 T5702 punct .,recognized
R3424 T5740 T5741 advmod Therefore,influence
R3425 T5742 T5741 punct ", ",influence
R3426 T5743 T5744 det the,loss
R3427 T5744 T5741 nsubj loss,influence
R3428 T5745 T5744 prep of,loss
R3429 T5746 T5745 pobj Sam68,of
R343 T1061 T1058 punct ", ",favored
R3430 T5747 T5741 aux could,influence
R3431 T5748 T5749 det the,cells
R3432 T5749 T5741 dobj cells,influence
R3433 T5750 T5751 nmod bone,marrow
R3434 T5751 T5749 nmod marrow,cells
R3435 T5752 T5749 amod stromal,cells
R3436 T5753 T5754 aux to,differentiate
R3437 T5754 T5741 advcl differentiate,influence
R3438 T5755 T5754 prep along,differentiate
R3439 T5756 T5757 det the,osteogenic
R344 T1062 T1063 det the,activity
R3440 T5757 T5755 pobj osteogenic,along
R3441 T5758 T5757 cc versus,osteogenic
R3442 T5759 T5760 det the,adipogenic
R3443 T5760 T5761 nmod adipogenic,pathway
R3444 T5761 T5757 conj pathway,osteogenic
R3445 T5762 T5741 punct .,influence
R3446 T5764 T5765 advmod Alternatively,regulate
R3447 T5766 T5765 punct ", ",regulate
R3448 T5767 T5768 det the,loss
R3449 T5768 T5765 nsubj loss,regulate
R345 T1063 T1058 nsubjpass activity,favored
R3450 T5769 T5768 prep of,loss
R3451 T5770 T5769 pobj Sam68,of
R3452 T5771 T5765 aux could,regulate
R3453 T5772 T5765 advmod indirectly,regulate
R3454 T5773 T5774 compound bone,mass
R3455 T5774 T5765 dobj mass,regulate
R3456 T5775 T5765 prep as,regulate
R3457 T5776 T5777 det a,disturbance
R3458 T5777 T5775 pobj disturbance,as
R3459 T5778 T5777 amod general,disturbance
R346 T1064 T1063 amod anabolic,activity
R3460 T5779 T5777 prep of,disturbance
R3461 T5780 T5781 amod neuroendocrine,control
R3462 T5781 T5779 pobj control,of
R3463 T5782 T5783 mark as,shown
R3464 T5783 T5765 advcl shown,regulate
R3465 T5784 T5783 auxpass was,shown
R3466 T5785 T5786 advmod when,shown
R3467 T5786 T5783 advcl shown,shown
R3468 T5787 T5786 nsubjpass leptin,shown
R3469 T5788 T5787 cc and,leptin
R347 T1065 T1063 prep of,activity
R3470 T5789 T5790 det the,axis
R3471 T5790 T5787 conj axis,leptin
R3472 T5791 T5790 amod sympathetic,axis
R3473 T5792 T5793 amod nervous,system
R3474 T5793 T5790 compound system,axis
R3475 T5794 T5786 auxpass were,shown
R3476 T5795 T5796 aux to,regulate
R3477 T5796 T5786 xcomp regulate,shown
R3478 T5797 T5796 advmod negatively,regulate
R3479 T5798 T5799 compound bone,mass
R348 T1066 T1065 pobj osteoblasts,of
R3480 T5799 T5796 dobj mass,regulate
R3481 T5800 T5801 punct [,8
R3482 T5801 T5765 parataxis 8,regulate
R3483 T5802 T5801 punct ],8
R3484 T5803 T5765 punct .,regulate
R3485 T5805 T5806 aux To,confirm
R3486 T5806 T5807 advcl confirm,isolated
R3487 T5808 T5809 det a,role
R3488 T5809 T5806 dobj role,confirm
R3489 T5810 T5809 prep for,role
R349 T1067 T1068 punct [,1
R3490 T5811 T5810 pobj Sam68,for
R3491 T5812 T5809 prep in,role
R3492 T5813 T5814 det the,regulation
R3493 T5814 T5812 pobj regulation,in
R3494 T5815 T5814 prep of,regulation
R3495 T5816 T5817 compound adipocyte,differentiation
R3496 T5817 T5815 pobj differentiation,of
R3497 T5818 T5807 punct ", ",isolated
R3498 T5819 T5807 nsubj we,isolated
R3499 T5820 T5821 amod primary,MEFs
R350 T1068 T1063 parataxis 1,activity
R3500 T5821 T5807 dobj MEFs,isolated
R3501 T5822 T5821 prep from,MEFs
R3502 T5823 T5824 nummod 14.5,day
R3503 T5824 T5826 npadvmod day,old
R3504 T5825 T5824 punct -,day
R3505 T5826 T5828 amod old,embryos
R3506 T5827 T5826 punct -,old
R3507 T5828 T5822 pobj embryos,from
R3508 T5829 T5828 nmod Sam68,embryos
R3509 T5830 T5829 punct +,Sam68
R351 T1069 T1068 punct ],1
R3510 T5831 T5829 punct /,Sam68
R3511 T5832 T5829 punct +,Sam68
R3512 T5833 T5829 cc and,Sam68
R3513 T5834 T5829 conj Sam68,Sam68
R3514 T5835 T5834 punct −,Sam68
R3515 T5836 T5834 punct /,Sam68
R3516 T5837 T5834 punct −,Sam68
R3517 T5838 T5807 punct .,isolated
R3518 T5840 T5841 det The,MEFs
R3519 T5841 T5847 nsubjpass MEFs,differentiated
R352 T1070 T1058 auxpass is,favored
R3520 T5842 T5841 amod primary,MEFs
R3521 T5843 T5841 nmod Sam68,MEFs
R3522 T5844 T5843 punct −,Sam68
R3523 T5845 T5843 punct /,Sam68
R3524 T5846 T5843 punct −,Sam68
R3525 T5848 T5847 auxpass were,differentiated
R3526 T5849 T5847 prep into,differentiated
R3527 T5850 T5849 pobj adipocytes,into
R3528 T5851 T5852 advmod in,vitro
R3529 T5852 T5847 advmod vitro,differentiated
R353 T1071 T1058 prep over,favored
R3530 T5853 T5847 prep in,differentiated
R3531 T5854 T5855 compound culture,medium
R3532 T5855 T5853 pobj medium,in
R3533 T5856 T5855 acl containing,medium
R3534 T5857 T5858 nummod 5,μM
R3535 T5858 T5859 compound μM,pioglitazone
R3536 T5859 T5856 dobj pioglitazone,containing
R3537 T5860 T5861 aux to,induce
R3538 T5861 T5847 advcl induce,differentiated
R3539 T5862 T5861 dobj adipogenesis,induce
R354 T1072 T1073 det the,activity
R3540 T5863 T5847 punct .,differentiated
R3541 T5865 T5866 nsubjpass Cells,stained
R3542 T5867 T5865 prep at,Cells
R3543 T5868 T5869 nmod days,0
R3544 T5869 T5867 pobj 0,at
R3545 T5870 T5869 punct ", ",0
R3546 T5871 T5869 conj 4,0
R3547 T5872 T5871 punct ", ",4
R3548 T5873 T5871 conj 6,4
R3549 T5874 T5873 punct ", ",6
R355 T1073 T1071 pobj activity,over
R3550 T5875 T5873 cc and,6
R3551 T5876 T5873 conj 12,6
R3552 T5877 T5866 auxpass were,stained
R3553 T5878 T5866 prep with,stained
R3554 T5879 T5880 compound Oil,O
R3555 T5880 T5878 pobj O,with
R3556 T5881 T5880 compound red,O
R3557 T5882 T5883 aux to,monitor
R3558 T5883 T5866 advcl monitor,stained
R3559 T5884 T5883 dobj adipogenesis,monitor
R356 T1074 T1073 amod catabolic,activity
R3560 T5885 T5866 punct .,stained
R3561 T5887 T5888 nsubj Adipogenesis,was
R3562 T5889 T5890 advmod more,pronounced
R3563 T5890 T5888 acomp pronounced,was
R3564 T5891 T5888 prep in,was
R3565 T5892 T5893 det the,cultures
R3566 T5893 T5891 pobj cultures,in
R3567 T5894 T5895 amod wild,type
R3568 T5895 T5893 compound type,cultures
R3569 T5896 T5895 punct -,type
R357 T1075 T1073 prep of,activity
R3570 T5897 T5893 compound MEFs,cultures
R3571 T5898 T5888 prep than,was
R3572 T5899 T5898 prep in,than
R3573 T5900 T5901 det the,MEFs
R3574 T5901 T5899 pobj MEFs,in
R3575 T5902 T5901 nmod Sam68,MEFs
R3576 T5903 T5902 punct −,Sam68
R3577 T5904 T5902 punct /,Sam68
R3578 T5905 T5902 punct −,Sam68
R3579 T5906 T5888 punct ", ",was
R358 T1076 T1075 pobj osteoclasts,of
R3580 T5907 T5888 advcl consistent,was
R3581 T5908 T5907 prep with,consistent
R3582 T5909 T5910 det the,role
R3583 T5910 T5908 pobj role,with
R3584 T5911 T5910 amod positive,role
R3585 T5912 T5910 prep of,role
R3586 T5913 T5914 amod endogenous,Sam68
R3587 T5914 T5912 pobj Sam68,of
R3588 T5915 T5910 prep in,role
R3589 T5916 T5917 compound adipocyte,differentiation
R359 T1077 T1078 punct [,2
R3590 T5917 T5915 pobj differentiation,in
R3591 T5918 T5919 punct (,Figure
R3592 T5919 T5888 parataxis Figure,was
R3593 T5920 T5919 nummod 7,Figure
R3594 T5921 T5919 punct ),Figure
R3595 T5922 T5888 punct .,was
R3596 T5924 T5925 det The,expression
R3597 T5925 T5926 nsubjpass expression,impaired
R3598 T5927 T5925 prep of,expression
R3599 T5928 T5929 amod key,factors
R360 T1078 T1073 appos 2,activity
R3600 T5929 T5927 pobj factors,of
R3601 T5930 T5929 compound transcription,factors
R3602 T5931 T5929 prep including,factors
R3603 T5932 T5933 det the,PPARγ
R3604 T5933 T5931 pobj PPARγ,including
R3605 T5934 T5933 cc and,PPARγ
R3606 T5935 T5933 conj KLF5,PPARγ
R3607 T5936 T5926 auxpass was,impaired
R3608 T5937 T5926 prep in,impaired
R3609 T5938 T5939 nmod Sam68,MEFs
R361 T1079 T1078 punct ],2
R3610 T5939 T5937 pobj MEFs,in
R3611 T5940 T5938 punct −,Sam68
R3612 T5941 T5938 punct /,Sam68
R3613 T5942 T5938 punct −,Sam68
R3614 T5943 T5939 amod differentiated,MEFs
R3615 T5944 T5926 prep compared,impaired
R3616 T5945 T5944 prep with,compared
R3617 T5946 T5947 nmod Sam68,MEFs
R3618 T5947 T5945 pobj MEFs,with
R3619 T5948 T5946 punct +,Sam68
R362 T1080 T1073 punct ", ",activity
R3620 T5949 T5946 punct /,Sam68
R3621 T5950 T5946 punct +,Sam68
R3622 T5951 T5926 punct ", ",impaired
R3623 T5952 T5926 advcl consistent,impaired
R3624 T5953 T5952 prep with,consistent
R3625 T5954 T5955 amod impaired,adipogenesis
R3626 T5955 T5953 pobj adipogenesis,with
R3627 T5956 T5955 prep in,adipogenesis
R3628 T5957 T5958 det the,absence
R3629 T5958 T5956 pobj absence,in
R363 T1081 T1082 dep which,results
R3630 T5959 T5958 prep of,absence
R3631 T5960 T5959 pobj Sam68,of
R3632 T5961 T5962 punct (,Figure
R3633 T5962 T5926 parataxis Figure,impaired
R3634 T5963 T5962 nummod 7,Figure
R3635 T5964 T5962 punct ),Figure
R3636 T5965 T5926 punct .,impaired
R3637 T5967 T5968 det These,data
R3638 T5968 T5969 nsubj data,support
R3639 T5970 T5968 punct ", ",data
R364 T1082 T1073 relcl results,activity
R3640 T5971 T5968 advmod together,data
R3641 T5972 T5971 prep with,together
R3642 T5973 T5972 pobj data,with
R3643 T5974 T5973 acl confirming,data
R3644 T5975 T5976 det a,phenotype
R3645 T5976 T5974 dobj phenotype,confirming
R3646 T5977 T5976 amod lean,phenotype
R3647 T5978 T5976 prep in,phenotype
R3648 T5979 T5980 nmod Sam68,mice
R3649 T5980 T5978 pobj mice,in
R365 T1083 T1082 prep in,results
R3650 T5981 T5979 punct −,Sam68
R3651 T5982 T5979 punct /,Sam68
R3652 T5983 T5979 punct −,Sam68
R3653 T5984 T5985 punct (,N.
R3654 T5985 T5968 meta N.,data
R3655 T5986 T5985 nmod Torabi,N.
R3656 T5987 T5985 cc and,N.
R3657 T5988 T5985 conj S.,N.
R3658 T5989 T5988 nmod Richard,S.
R3659 T5990 T5988 punct ", ",S.
R366 T1084 T1085 det a,gain
R3660 T5991 T5992 amod unpublished,data
R3661 T5992 T5988 conj data,S.
R3662 T5993 T5992 punct ),data
R3663 T5994 T5969 punct ", ",support
R3664 T5995 T5996 det the,hypothesis
R3665 T5996 T5969 dobj hypothesis,support
R3666 T5997 T5998 mark that,modulates
R3667 T5998 T5996 acl modulates,hypothesis
R3668 T5999 T5998 nsubj Sam68,modulates
R3669 T6000 T6001 det the,differentiation
R367 T1085 T1083 pobj gain,in
R3670 T6001 T5998 dobj differentiation,modulates
R3671 T6002 T6001 prep of,differentiation
R3672 T6003 T6004 amod mesenchymal,cells
R3673 T6004 T6002 pobj cells,of
R3674 T6005 T5969 punct .,support
R3675 T6007 T6008 aux To,examine
R3676 T6008 T6010 advcl examine,chose
R3677 T6009 T6008 advmod further,examine
R3678 T6011 T6012 det this,phenotype
R3679 T6012 T6008 dobj phenotype,examine
R368 T1086 T1085 amod net,gain
R3680 T6013 T6008 prep in,examine
R3681 T6014 T6015 det a,system
R3682 T6015 T6013 pobj system,in
R3683 T6016 T6017 npadvmod cell,autonomous
R3684 T6017 T6015 amod autonomous,system
R3685 T6018 T6010 punct ", ",chose
R3686 T6019 T6010 nsubj we,chose
R3687 T6020 T6021 det the,cells
R3688 T6021 T6010 dobj cells,chose
R3689 T6022 T6021 amod embryonic,cells
R369 T1087 T1085 prep in,gain
R3690 T6023 T6021 amod mesenchymal,cells
R3691 T6024 T6021 amod multipotential,cells
R3692 T6025 T6021 compound progenitor,cells
R3693 T6026 T6021 appos C3H10T1,cells
R3694 T6027 T6026 punct /,C3H10T1
R3695 T6028 T6026 nummod 2,C3H10T1
R3696 T6029 T6010 punct .,chose
R3697 T6031 T6032 det The,addition
R3698 T6032 T6033 nsubj addition,induced
R3699 T6034 T6032 prep of,addition
R370 T1088 T1089 compound bone,mass
R3700 T6035 T6034 pobj BMP,of
R3701 T6036 T6035 punct -,BMP
R3702 T6037 T6035 nummod 2,BMP
R3703 T6038 T6039 compound osteoblast,differentiation
R3704 T6039 T6033 dobj differentiation,induced
R3705 T6040 T6033 punct ", ",induced
R3706 T6041 T6042 mark as,evidenced
R3707 T6042 T6033 advcl evidenced,induced
R3708 T6043 T6042 agent by,evidenced
R3709 T6044 T6045 det an,increase
R371 T1089 T1087 pobj mass,in
R3710 T6045 T6043 pobj increase,by
R3711 T6046 T6047 advmod approximately,400-fold
R3712 T6047 T6045 nmod 400-fold,increase
R3713 T6048 T6045 prep in,increase
R3714 T6049 T6048 pobj expression,in
R3715 T6050 T6049 prep of,expression
R3716 T6051 T6052 det the,gene
R3717 T6052 T6050 pobj gene,of
R3718 T6053 T6052 nmod osteocalcin,gene
R3719 T6054 T6053 punct (,osteocalcin
R372 T1090 T1058 punct .,favored
R3720 T6055 T6053 appos OCN,osteocalcin
R3721 T6056 T6052 punct ),gene
R3722 T6057 T6058 punct [,51
R3723 T6058 T6033 parataxis 51,induced
R3724 T6059 T6058 punct ],51
R3725 T6060 T6033 punct .,induced
R3726 T6062 T6063 nsubjpass Populations,selected
R3727 T6064 T6062 prep of,Populations
R3728 T6065 T6066 nmod C3H10T1,cells
R3729 T6066 T6064 pobj cells,of
R373 T1092 T1093 prep At,maintained
R3730 T6067 T6065 punct /,C3H10T1
R3731 T6068 T6065 nummod 2,C3H10T1
R3732 T6069 T6070 advmod stably,transfected
R3733 T6070 T6062 acl transfected,Populations
R3734 T6071 T6070 prep with,transfected
R3735 T6072 T6073 preconj either,retro
R3736 T6073 T6071 pobj retro,with
R3737 T6074 T6073 compound pSuper,retro
R3738 T6075 T6073 punct -,retro
R3739 T6076 T6077 punct (,control
R374 T1094 T1095 amod skeletal,maturity
R3740 T6077 T6073 parataxis control,retro
R3741 T6078 T6077 punct ),control
R3742 T6079 T6073 cc and,retro
R3743 T6080 T6081 compound pSuper,retro
R3744 T6081 T6073 conj retro,retro
R3745 T6082 T6081 punct -,retro
R3746 T6083 T6081 acl harboring,retro
R3747 T6084 T6085 det a,hairpin
R3748 T6085 T6083 dobj hairpin,harboring
R3749 T6086 T6085 amod short,hairpin
R375 T1095 T1092 pobj maturity,At
R3750 T6087 T6085 prep against,hairpin
R3751 T6088 T6087 pobj Sam68,against
R3752 T6089 T6085 punct (,hairpin
R3753 T6090 T6085 appos Sam68sh,hairpin
R3754 T6091 T6063 punct ),selected
R3755 T6092 T6063 auxpass were,selected
R3756 T6093 T6063 prep with,selected
R3757 T6094 T6093 pobj puromycin,with
R3758 T6095 T6063 punct .,selected
R3759 T6097 T6098 det The,expression
R376 T1096 T1093 punct ", ",maintained
R3760 T6098 T6099 nsubjpass expression,reduced
R3761 T6100 T6098 prep of,expression
R3762 T6101 T6100 pobj Sam68,of
R3763 T6102 T6099 auxpass was,reduced
R3764 T6103 T6099 prep by,reduced
R3765 T6104 T6105 advmod approximately,80
R3766 T6105 T6106 nummod 80,%
R3767 T6106 T6103 pobj %,by
R3768 T6107 T6108 mark as,evidenced
R3769 T6108 T6099 advcl evidenced,reduced
R377 T1097 T1098 compound bone,mass
R3770 T6109 T6108 agent by,evidenced
R3771 T6110 T6111 compound immunoblot,analyses
R3772 T6111 T6109 pobj analyses,by
R3773 T6112 T6111 acl using,analyses
R3774 T6113 T6114 compound β,actin
R3775 T6114 T6112 dobj actin,using
R3776 T6115 T6114 punct -,actin
R3777 T6116 T6112 prep as,using
R3778 T6117 T6118 det a,control
R3779 T6118 T6116 pobj control,as
R378 T1098 T1093 nsubjpass mass,maintained
R3780 T6119 T6118 compound loading,control
R3781 T6120 T6121 punct (,8A
R3782 T6121 T6099 parataxis 8A,reduced
R3783 T6122 T6121 compound Figure,8A
R3784 T6123 T6121 punct ),8A
R3785 T6124 T6099 punct .,reduced
R3786 T6126 T6127 compound Osteoblast,differentiation
R3787 T6127 T6128 nsubjpass differentiation,induced
R3788 T6129 T6128 auxpass was,induced
R3789 T6130 T6128 prep in,induced
R379 T1099 T1093 auxpass is,maintained
R3790 T6131 T6130 pobj cultures,in
R3791 T6132 T6131 acl expressing,cultures
R3792 T6133 T6134 compound pSuper,retro
R3793 T6134 T6132 dobj retro,expressing
R3794 T6135 T6134 punct -,retro
R3795 T6136 T6134 cc or,retro
R3796 T6137 T6138 compound pSuper,retro
R3797 T6138 T6140 compound retro,Sam68sh
R3798 T6139 T6138 punct -,retro
R3799 T6140 T6134 conj Sam68sh,retro
R380 T1100 T1093 prep through,maintained
R3800 T6141 T6128 prep by,induced
R3801 T6142 T6141 pobj addition,by
R3802 T6143 T6142 prep of,addition
R3803 T6144 T6143 pobj BMP,of
R3804 T6145 T6144 punct -,BMP
R3805 T6146 T6144 nummod 2,BMP
R3806 T6147 T6142 prep to,addition
R3807 T6148 T6149 det the,medium
R3808 T6149 T6147 pobj medium,to
R3809 T6150 T6149 compound culture,medium
R381 T1101 T1102 det the,activity
R3810 T6151 T6128 punct .,induced
R3811 T6152 T6128 punct .,induced
R3812 T6154 T6155 det The,expression
R3813 T6155 T6156 nsubjpass expression,examined
R3814 T6157 T6155 prep of,expression
R3815 T6158 T6157 pobj OCN,of
R3816 T6159 T6158 cc and,OCN
R3817 T6160 T6161 compound β,actin
R3818 T6161 T6163 compound actin,mRNAs
R3819 T6162 T6161 punct -,actin
R382 T1102 T1100 pobj activity,through
R3820 T6163 T6158 conj mRNAs,OCN
R3821 T6164 T6156 auxpass was,examined
R3822 T6165 T6156 prep by,examined
R3823 T6166 T6167 amod semiquantitative,PCR
R3824 T6167 T6165 pobj PCR,by
R3825 T6168 T6167 compound RT,PCR
R3826 T6169 T6167 punct -,PCR
R3827 T6170 T6156 cc and,examined
R3828 T6171 T6172 det the,cells
R3829 T6172 T6176 nsubj cells,displayed
R383 T1103 T1102 amod balanced,activity
R3830 T6173 T6174 npadvmod Sam68sh,expressing
R3831 T6174 T6172 amod expressing,cells
R3832 T6175 T6174 punct -,expressing
R3833 T6176 T6156 conj displayed,examined
R3834 T6177 T6178 det a,phenotype
R3835 T6178 T6176 dobj phenotype,displayed
R3836 T6179 T6180 advmod more,pronounced
R3837 T6180 T6178 amod pronounced,phenotype
R3838 T6181 T6178 compound osteoblast,phenotype
R3839 T6182 T6178 prep compared,phenotype
R384 T1104 T1102 prep of,activity
R3840 T6183 T6182 prep with,compared
R3841 T6184 T6185 compound control,cells
R3842 T6185 T6183 pobj cells,with
R3843 T6186 T6176 punct ", ",displayed
R3844 T6187 T6188 mark as,assessed
R3845 T6188 T6176 advcl assessed,displayed
R3846 T6189 T6188 prep by,assessed
R3847 T6190 T6191 det the,expression
R3848 T6191 T6189 pobj expression,by
R3849 T6192 T6191 prep of,expression
R385 T1105 T1104 pobj osteoblasts,of
R3850 T6193 T6192 pobj OCN,of
R3851 T6194 T6195 punct (,8B
R3852 T6195 T6176 parataxis 8B,displayed
R3853 T6196 T6195 compound Figure,8B
R3854 T6197 T6195 punct ),8B
R3855 T6198 T6176 punct .,displayed
R3856 T6200 T6201 prep Given,stained
R3857 T6202 T6203 det the,enhancement
R3858 T6203 T6200 pobj enhancement,Given
R3859 T6204 T6203 amod apparent,enhancement
R386 T1106 T1105 cc and,osteoblasts
R3860 T6205 T6203 prep of,enhancement
R3861 T6206 T6207 amod mineralized,nodule
R3862 T6207 T6208 compound nodule,formation
R3863 T6208 T6205 pobj formation,of
R3864 T6209 T6203 prep by,enhancement
R3865 T6210 T6211 nmod Sam68,cells
R3866 T6211 T6209 pobj cells,by
R3867 T6212 T6210 punct −,Sam68
R3868 T6213 T6210 punct /,Sam68
R3869 T6214 T6210 punct −,Sam68
R387 T1107 T1105 conj osteoclasts,osteoblasts
R3870 T6215 T6216 nmod bone,marrow
R3871 T6216 T6211 nmod marrow,cells
R3872 T6217 T6211 amod stromal,cells
R3873 T6218 T6219 advmod ex,vivo
R3874 T6219 T6203 advmod vivo,enhancement
R3875 T6220 T6203 cc and,enhancement
R3876 T6221 T6222 det the,phenotype
R3877 T6222 T6203 conj phenotype,enhancement
R3878 T6223 T6222 acl observed,phenotype
R3879 T6224 T6223 prep with,observed
R388 T1108 T1093 prep during,maintained
R3880 T6225 T6226 amod short,RNA
R3881 T6226 T6228 npadvmod RNA,treated
R3882 T6227 T6226 compound hairpin,RNA
R3883 T6228 T6233 amod treated,C3H10T1
R3884 T6229 T6226 punct (,RNA
R3885 T6230 T6226 appos shRNA,RNA
R3886 T6231 T6228 punct ),treated
R3887 T6232 T6228 punct -,treated
R3888 T6233 T6224 pobj C3H10T1,with
R3889 T6234 T6233 punct /,C3H10T1
R389 T1109 T1110 det the,cycle
R3890 T6235 T6233 nummod 2,C3H10T1
R3891 T6236 T6201 punct ", ",stained
R3892 T6237 T6201 nsubj we,stained
R3893 T6238 T6201 dobj sections,stained
R3894 T6239 T6238 prep of,sections
R3895 T6240 T6239 pobj bone,of
R3896 T6241 T6238 prep from,sections
R3897 T6242 T6243 nummod 4,month
R3898 T6243 T6248 npadvmod month,old
R3899 T6244 T6242 punct -,4
R390 T1110 T1108 pobj cycle,during
R3900 T6245 T6242 cc and,4
R3901 T6246 T6242 conj 12,4
R3902 T6247 T6243 punct -,month
R3903 T6248 T6250 amod old,mice
R3904 T6249 T6248 punct -,old
R3905 T6250 T6241 pobj mice,from
R3906 T6251 T6201 prep for,stained
R3907 T6252 T6251 pobj evidence,for
R3908 T6253 T6252 prep of,evidence
R3909 T6254 T6253 pobj changes,of
R391 T1111 T1110 compound remodeling,cycle
R3910 T6255 T6254 prep in,changes
R3911 T6256 T6257 compound marrow,adiposity
R3912 T6257 T6255 pobj adiposity,in
R3913 T6258 T6201 punct .,stained
R3914 T6260 T6261 nsubj Figure,shows
R3915 T6262 T6260 nummod 9,Figure
R3916 T6263 T6261 dobj sections,shows
R3917 T6264 T6263 prep of,sections
R3918 T6265 T6266 amod undecalcified,bone
R3919 T6266 T6264 pobj bone,of
R392 T1112 T1093 punct .,maintained
R3920 T6267 T6266 prep from,bone
R3921 T6268 T6269 nummod 4,month
R3922 T6269 T6274 npadvmod month,old
R3923 T6270 T6268 punct -,4
R3924 T6271 T6268 cc and,4
R3925 T6272 T6268 conj 12,4
R3926 T6273 T6269 punct -,month
R3927 T6274 T6276 amod old,mice
R3928 T6275 T6274 punct -,old
R3929 T6276 T6267 pobj mice,from
R393 T1114 T1115 prep During,is
R3930 T6277 T6276 nmod Sam68,mice
R3931 T6278 T6277 punct +,Sam68
R3932 T6279 T6277 punct /,Sam68
R3933 T6280 T6277 punct +,Sam68
R3934 T6281 T6277 cc and,Sam68
R3935 T6282 T6277 conj Sam68,Sam68
R3936 T6283 T6282 punct −,Sam68
R3937 T6284 T6282 punct /,Sam68
R3938 T6285 T6282 punct −,Sam68
R3939 T6286 T6276 acl stained,mice
R394 T1116 T1117 amod skeletal,aging
R3940 T6287 T6286 prep with,stained
R3941 T6288 T6289 compound von,Kossa
R3942 T6289 T6287 pobj Kossa,with
R3943 T6290 T6289 cc and,Kossa
R3944 T6291 T6289 conj toluidine,Kossa
R3945 T6292 T6291 amod blue,toluidine
R3946 T6293 T6294 aux to,show
R3947 T6294 T6286 advcl show,stained
R3948 T6295 T6296 amod mineralized,tissue
R3949 T6296 T6294 dobj tissue,show
R395 T1117 T1114 pobj aging,During
R3950 T6297 T6298 punct (,9A
R3951 T6298 T6296 parataxis 9A,tissue
R3952 T6299 T6298 compound Figure,9A
R3953 T6300 T6298 punct ", ",9A
R3954 T6301 T6298 amod black,9A
R3955 T6302 T6298 punct ),9A
R3956 T6303 T6296 cc and,tissue
R3957 T6304 T6305 compound marrow,adipocytes
R3958 T6305 T6296 conj adipocytes,tissue
R3959 T6306 T6307 punct (,9B
R396 T1118 T1115 punct ", ",is
R3960 T6307 T6305 parataxis 9B,adipocytes
R3961 T6308 T6307 compound Figure,9B
R3962 T6309 T6307 punct ", ",9B
R3963 T6310 T6307 amod white,9B
R3964 T6311 T6307 punct ),9B
R3965 T6312 T6286 cc and,stained
R3966 T6313 T6286 conj left,stained
R3967 T6314 T6313 oprd unstained,left
R3968 T6315 T6316 aux to,show
R3969 T6316 T6313 advcl show,left
R397 T1119 T1115 expl there,is
R3970 T6317 T6318 compound fluorochrome,labeling
R3971 T6318 T6316 dobj labeling,show
R3972 T6319 T6318 prep of,labeling
R3973 T6320 T6321 det the,fronts
R3974 T6321 T6319 pobj fronts,of
R3975 T6322 T6321 compound mineralization,fronts
R3976 T6323 T6324 punct (,9C
R3977 T6324 T6316 parataxis 9C,show
R3978 T6325 T6324 compound Figure,9C
R3979 T6326 T6324 punct ),9C
R398 T1120 T1121 det a,shift
R3980 T6327 T6261 punct .,shows
R3981 T6329 T6330 nummod Twelve,month
R3982 T6330 T6332 npadvmod month,old
R3983 T6331 T6330 punct -,month
R3984 T6332 T6334 amod old,mice
R3985 T6333 T6332 punct -,old
R3986 T6334 T6339 nsubj mice,showed
R3987 T6335 T6334 nmod Sam68,mice
R3988 T6336 T6335 punct +,Sam68
R3989 T6337 T6335 punct /,Sam68
R399 T1121 T1115 attr shift,is
R3990 T6338 T6335 punct +,Sam68
R3991 T6340 T6341 det a,decrease
R3992 T6341 T6339 dobj decrease,showed
R3993 T6342 T6341 amod noticeable,decrease
R3994 T6343 T6341 prep in,decrease
R3995 T6344 T6345 amod trabecular,bone
R3996 T6345 T6343 pobj bone,in
R3997 T6346 T6347 punct (,9A
R3998 T6347 T6345 parataxis 9A,bone
R3999 T6348 T6347 compound Figure,9A
R400 T1122 T1121 prep in,shift
R4000 T6349 T6347 punct ),9A
R4001 T6350 T6341 punct ", ",decrease
R4002 T6351 T6352 dep which,associated
R4003 T6352 T6341 relcl associated,decrease
R4004 T6353 T6352 auxpass was,associated
R4005 T6354 T6352 prep with,associated
R4006 T6355 T6356 det a,increase
R4007 T6356 T6354 pobj increase,with
R4008 T6357 T6356 amod significant,increase
R4009 T6358 T6356 prep in,increase
R401 T1123 T1124 det the,balance
R4010 T6359 T6360 compound marrow,adiposity
R4011 T6360 T6358 pobj adiposity,in
R4012 T6361 T6362 punct (,9B
R4013 T6362 T6356 parataxis 9B,increase
R4014 T6363 T6362 compound Figure,9B
R4015 T6364 T6362 punct ),9B
R4016 T6365 T6354 cc and,with
R4017 T6366 T6354 conj with,with
R4018 T6367 T6368 det the,labels
R4019 T6368 T6366 pobj labels,with
R402 T1124 T1122 pobj balance,in
R4020 T6369 T6368 nummod two,labels
R4021 T6370 T6368 compound fluorochrome,labels
R4022 T6371 T6368 acl superimposed,labels
R4023 T6372 T6371 prep upon,superimposed
R4024 T6373 T6372 pobj one,upon
R4025 T6374 T6373 det another,one
R4026 T6375 T6376 punct (,9C
R4027 T6376 T6368 parataxis 9C,labels
R4028 T6377 T6376 compound Figure,9C
R4029 T6378 T6376 punct ),9C
R403 T1125 T1126 dep that,favors
R4030 T6379 T6339 punct .,showed
R4031 T6381 T6382 prep In,appeared
R4032 T6383 T6381 pobj contrast,In
R4033 T6384 T6382 punct ", ",appeared
R4034 T6385 T6386 det the,bones
R4035 T6386 T6382 nsubj bones,appeared
R4036 T6387 T6386 prep of,bones
R4037 T6388 T6389 nummod 12,month
R4038 T6389 T6391 npadvmod month,old
R4039 T6390 T6389 punct -,month
R404 T1126 T1121 relcl favors,shift
R4040 T6391 T6393 amod old,mice
R4041 T6392 T6391 punct -,old
R4042 T6393 T6387 pobj mice,of
R4043 T6394 T6393 nmod Sam68,mice
R4044 T6395 T6394 punct −,Sam68
R4045 T6396 T6394 punct /,Sam68
R4046 T6397 T6394 punct −,Sam68
R4047 T6398 T6382 oprd similar,appeared
R4048 T6399 T6398 prep to,similar
R4049 T6400 T6399 pobj those,to
R405 T1127 T1126 dative osteoclast,favors
R4050 T6401 T6400 prep of,those
R4051 T6402 T6403 det the,mice
R4052 T6403 T6401 pobj mice,of
R4053 T6404 T6405 nummod 4,month
R4054 T6405 T6407 npadvmod month,old
R4055 T6406 T6405 punct -,month
R4056 T6407 T6403 amod old,mice
R4057 T6408 T6407 punct -,old
R4058 T6409 T6403 prep of,mice
R4059 T6410 T6411 det either,genotype
R406 T1128 T1126 prep over,favors
R4060 T6411 T6409 pobj genotype,of
R4061 T6412 T6382 punct .,appeared
R4062 T6414 T6415 det These,data
R4063 T6415 T6416 nsubj data,demonstrate
R4064 T6417 T6418 mark that,regulates
R4065 T6418 T6416 ccomp regulates,demonstrate
R4066 T6419 T6418 nsubj Sam68,regulates
R4067 T6420 T6421 det the,differentiation
R4068 T6421 T6418 dobj differentiation,regulates
R4069 T6422 T6421 prep of,differentiation
R407 T1129 T1128 pobj osteoblast,over
R4070 T6423 T6424 nmod bone,marrow
R4071 T6424 T6425 nmod marrow,cells
R4072 T6425 T6422 pobj cells,of
R4073 T6426 T6425 amod mesenchymal,cells
R4074 T6427 T6428 aux to,promote
R4075 T6428 T6418 advcl promote,regulates
R4076 T6429 T6430 compound adipocyte,differentiation
R4077 T6430 T6428 dobj differentiation,promote
R4078 T6431 T6428 cc and,promote
R4079 T6432 T6428 conj inhibit,promote
R408 T1130 T1126 dobj activity,favors
R4080 T6433 T6434 compound osteoblast,differentiation
R4081 T6434 T6432 dobj differentiation,inhibit
R4082 T6435 T6428 prep in,promote
R4083 T6436 T6437 amod aging,bone
R4084 T6437 T6435 pobj bone,in
R4085 T6438 T6416 punct .,demonstrate
R409 T1131 T1126 punct ", ",favors
R410 T1132 T1133 dep which,results
R4104 T7333 T7334 det The,study
R4105 T7334 T7336 nsubj study,provides
R4106 T7335 T7334 amod present,study
R4107 T7337 T7336 dobj evidence,provides
R4108 T7338 T7339 mark that,is
R4109 T7339 T7337 acl is,evidence
R411 T1133 T1126 ccomp results,favors
R4110 T7340 T7341 det a,role
R4111 T7341 T7339 nsubj role,is
R4112 T7342 T7341 amod physiologic,role
R4113 T7343 T7341 prep of,role
R4114 T7344 T7343 pobj Sam68,of
R4115 T7345 T7346 aux to,modulate
R4116 T7346 T7339 xcomp modulate,is
R4117 T7347 T7348 nmod bone,marrow
R4118 T7348 T7349 nmod marrow,cells
R4119 T7349 T7346 dobj cells,modulate
R412 T1134 T1133 prep in,results
R4120 T7350 T7349 amod mesenchymal,cells
R4121 T7351 T7349 compound stem,cells
R4122 T7352 T7336 punct .,provides
R4123 T7354 T7355 amod Young,mice
R4124 T7355 T7360 nsubj mice,developed
R4125 T7356 T7355 nmod Sam68,mice
R4126 T7357 T7356 punct −,Sam68
R4127 T7358 T7356 punct /,Sam68
R4128 T7359 T7356 punct −,Sam68
R4129 T7361 T7360 advmod normally,developed
R413 T1135 T1136 amod net,loss
R4130 T7362 T7360 cc and,developed
R4131 T7363 T7360 conj contained,developed
R4132 T7364 T7365 amod similar,mass
R4133 T7365 T7363 dobj mass,contained
R4134 T7366 T7365 compound bone,mass
R4135 T7367 T7363 prep compared,contained
R4136 T7368 T7367 prep with,compared
R4137 T7369 T7370 amod wild,type
R4138 T7370 T7372 compound type,littermates
R4139 T7371 T7370 punct -,type
R414 T1136 T1134 pobj loss,in
R4140 T7372 T7368 pobj littermates,with
R4141 T7373 T7360 punct .,developed
R4142 T7375 T7376 amod Aged,mice
R4143 T7376 T7385 nsubj mice,displayed
R4144 T7377 T7378 punct (,months
R4145 T7378 T7375 parataxis months,Aged
R4146 T7379 T7378 nummod 12,months
R4147 T7380 T7378 punct ),months
R4148 T7381 T7376 nmod Sam68,mice
R4149 T7382 T7381 punct −,Sam68
R415 T1137 T1136 compound bone,loss
R4150 T7383 T7381 punct /,Sam68
R4151 T7384 T7381 punct −,Sam68
R4152 T7386 T7387 det a,phenotype
R4153 T7387 T7385 dobj phenotype,displayed
R4154 T7388 T7389 amod high,mass
R4155 T7389 T7387 compound mass,phenotype
R4156 T7390 T7389 compound bone,mass
R4157 T7391 T7385 prep compared,displayed
R4158 T7392 T7391 prep with,compared
R4159 T7393 T7394 nmod Sam68,littermates
R416 T1138 T1139 punct [,3
R4160 T7394 T7392 pobj littermates,with
R4161 T7395 T7393 punct +,Sam68
R4162 T7396 T7393 punct /,Sam68
R4163 T7397 T7393 punct +,Sam68
R4164 T7398 T7385 punct .,displayed
R4165 T7400 T7401 det The,littermates
R4166 T7401 T7405 nsubj littermates,underwent
R4167 T7402 T7403 amod wild,type
R4168 T7403 T7401 compound type,littermates
R4169 T7404 T7403 punct -,type
R417 T1139 T1115 parataxis 3,is
R4170 T7406 T7407 npadvmod age,related
R4171 T7407 T7409 amod related,loss
R4172 T7408 T7407 punct -,related
R4173 T7409 T7405 dobj loss,underwent
R4174 T7410 T7409 compound bone,loss
R4175 T7411 T7412 dep that,occurs
R4176 T7412 T7409 relcl occurs,loss
R4177 T7413 T7412 advmod naturally,occurs
R4178 T7414 T7412 prep in,occurs
R4179 T7415 T7414 pobj mammals,in
R418 T1140 T1139 punct ],3
R4180 T7416 T7405 punct ", ",underwent
R4181 T7417 T7418 mark while,preserved
R4182 T7418 T7405 advcl preserved,underwent
R4183 T7419 T7420 det the,animals
R4184 T7420 T7418 nsubj animals,preserved
R4185 T7421 T7420 nmod Sam68,animals
R4186 T7422 T7421 punct −,Sam68
R4187 T7423 T7421 punct /,Sam68
R4188 T7424 T7421 punct −,Sam68
R4189 T7425 T7426 poss their,mass
R419 T1141 T1115 punct .,is
R4190 T7426 T7418 dobj mass,preserved
R4191 T7427 T7426 compound bone,mass
R4192 T7428 T7418 prep with,preserved
R4193 T7429 T7428 pobj aging,with
R4194 T7430 T7405 punct .,underwent
R4195 T7432 T7433 det The,differentiation
R4196 T7433 T7434 nsubj differentiation,resulted
R4197 T7435 T7433 prep of,differentiation
R4198 T7436 T7437 compound bone,marrow
R4199 T7437 T7438 compound marrow,cells
R420 T1143 T1144 det The,amount
R4200 T7438 T7435 pobj cells,of
R4201 T7439 T7438 compound stem,cells
R4202 T7440 T7438 acl isolated,cells
R4203 T7441 T7440 prep from,isolated
R4204 T7442 T7443 nmod Sam68,mice
R4205 T7443 T7441 pobj mice,from
R4206 T7444 T7442 punct −,Sam68
R4207 T7445 T7442 punct /,Sam68
R4208 T7446 T7442 punct −,Sam68
R4209 T7447 T7438 cc and,cells
R421 T1144 T1145 nsubjpass amount,determined
R4210 T7448 T7449 amod embryonic,cells
R4211 T7449 T7438 conj cells,cells
R4212 T7450 T7449 amod mesenchymal,cells
R4213 T7451 T7449 amod multipotential,cells
R4214 T7452 T7449 compound progenitor,cells
R4215 T7453 T7449 appos C3H10T1,cells
R4216 T7454 T7453 punct /,C3H10T1
R4217 T7455 T7453 nummod 2,C3H10T1
R4218 T7456 T7449 acl treated,cells
R4219 T7457 T7456 prep with,treated
R422 T1146 T1144 cc and,amount
R4220 T7458 T7459 compound Sam68,shRNA
R4221 T7459 T7457 pobj shRNA,with
R4222 T7460 T7434 prep in,resulted
R4223 T7461 T7462 det a,differentiation
R4224 T7462 T7460 pobj differentiation,in
R4225 T7463 T7464 advmod more,pronounced
R4226 T7464 T7462 amod pronounced,differentiation
R4227 T7465 T7462 compound osteoblast,differentiation
R4228 T7466 T7434 punct .,resulted
R4229 T7468 T7469 det These,findings
R423 T1147 T1144 conj rate,amount
R4230 T7469 T7470 nsubj findings,demonstrate
R4231 T7471 T7472 mark that,enhances
R4232 T7472 T7470 ccomp enhances,demonstrate
R4233 T7473 T7474 det the,loss
R4234 T7474 T7472 nsubj loss,enhances
R4235 T7475 T7474 prep of,loss
R4236 T7476 T7475 pobj Sam68,of
R4237 T7477 T7478 compound osteoblast,differentiation
R4238 T7478 T7472 dobj differentiation,enhances
R4239 T7479 T7470 punct .,demonstrate
R424 T1148 T1149 prep at,gained
R4240 T7481 T7482 det The,converse
R4241 T7482 T7483 nsubj converse,was
R4242 T7484 T7483 advmod also,was
R4243 T7485 T7483 acomp true,was
R4244 T7486 T7483 punct ", ",was
R4245 T7487 T7488 mark as,impaired
R4246 T7488 T7483 advcl impaired,was
R4247 T7489 T7488 nsubjpass MEFs,impaired
R4248 T7490 T7489 acl isolated,MEFs
R4249 T7491 T7490 prep from,isolated
R425 T1149 T1147 relcl gained,rate
R4250 T7492 T7493 nmod Sam68,animals
R4251 T7493 T7491 pobj animals,from
R4252 T7494 T7492 punct −,Sam68
R4253 T7495 T7492 punct /,Sam68
R4254 T7496 T7492 punct −,Sam68
R4255 T7497 T7488 auxpass were,impaired
R4256 T7498 T7488 prep in,impaired
R4257 T7499 T7500 poss their,ability
R4258 T7500 T7498 pobj ability,in
R4259 T7501 T7502 aux to,undergo
R426 T1150 T1148 pobj which,at
R4260 T7502 T7500 acl undergo,ability
R4261 T7503 T7504 compound adipocyte,differentiation
R4262 T7504 T7502 dobj differentiation,undergo
R4263 T7505 T7488 prep compared,impaired
R4264 T7506 T7505 prep with,compared
R4265 T7507 T7508 nmod Sam68,MEFs
R4266 T7508 T7506 pobj MEFs,with
R4267 T7509 T7507 punct +,Sam68
R4268 T7510 T7507 punct /,Sam68
R4269 T7511 T7507 punct +,Sam68
R427 T1151 T1149 nsubjpass bone,gained
R4270 T7512 T7483 punct .,was
R4271 T7514 T7515 det These,findings
R4272 T7515 T7516 nsubj findings,suggest
R4273 T7517 T7518 mark that,is
R4274 T7518 T7516 ccomp is,suggest
R4275 T7519 T7520 det a,role
R4276 T7520 T7518 nsubj role,is
R4277 T7521 T7520 amod physiologic,role
R4278 T7522 T7520 prep for,role
R4279 T7523 T7522 pobj Sam68,for
R428 T1152 T1149 auxpass is,gained
R4280 T7524 T7525 aux to,regulate
R4281 T7525 T7518 xcomp regulate,is
R4282 T7526 T7527 det the,balance
R4283 T7527 T7525 dobj balance,regulate
R4284 T7528 T7527 prep between,balance
R4285 T7529 T7530 amod adipogenic,differentiation
R4286 T7530 T7528 pobj differentiation,between
R4287 T7531 T7529 cc and,adipogenic
R4288 T7532 T7529 conj osteogenic,adipogenic
R4289 T7533 T7530 prep of,differentiation
R429 T1153 T1149 prep during,gained
R4290 T7534 T7535 det the,marrow
R4291 T7535 T7533 pobj marrow,of
R4292 T7536 T7535 compound bone,marrow
R4293 T7537 T7535 amod mesenchymal,marrow
R4294 T7538 T7516 punct .,suggest
R4295 T7540 T7541 compound Sam68,expression
R4296 T7541 T7543 nsubjpass expression,observed
R4297 T7542 T7541 compound protein,expression
R4298 T7544 T7543 auxpass was,observed
R4299 T7545 T7543 prep throughout,observed
R430 T1154 T1153 pobj development,during
R4300 T7546 T7547 det the,embryo
R4301 T7547 T7545 pobj embryo,throughout
R4302 T7548 T7547 amod developing,embryo
R4303 T7549 T7547 compound mouse,embryo
R4304 T7550 T7543 punct ", ",observed
R4305 T7551 T7543 prep in,observed
R4306 T7552 T7551 pobj keeping,in
R4307 T7553 T7552 prep with,keeping
R4308 T7554 T7555 amod previous,reports
R4309 T7555 T7553 pobj reports,with
R431 T1155 T1149 cc and,gained
R4310 T7556 T7557 dep that,identified
R4311 T7557 T7555 relcl identified,reports
R4312 T7558 T7559 det the,mRNA
R4313 T7559 T7561 nsubjpass mRNA,expressed
R4314 T7560 T7559 compound Sam68,mRNA
R4315 T7561 T7557 ccomp expressed,identified
R4316 T7562 T7561 aux to,expressed
R4317 T7563 T7561 auxpass be,expressed
R4318 T7564 T7561 advmod widely,expressed
R4319 T7565 T7566 punct [,38
R432 T1156 T1149 conj lost,gained
R4320 T7566 T7543 parataxis 38,observed
R4321 T7567 T7566 punct ],38
R4322 T7568 T7543 punct .,observed
R4323 T7570 T7571 nsubj We,observe
R4324 T7572 T7573 compound Sam68,staining
R4325 T7573 T7571 dobj staining,observe
R4326 T7574 T7571 prep in,observe
R4327 T7575 T7576 det the,brain
R4328 T7576 T7574 pobj brain,in
R4329 T7577 T7576 punct ", ",brain
R433 T1157 T1156 prep during,lost
R4330 T7578 T7576 conj heart,brain
R4331 T7579 T7578 punct ", ",heart
R4332 T7580 T7578 cc and,heart
R4333 T7581 T7578 conj intestine,heart
R4334 T7582 T7583 punct (,see
R4335 T7583 T7581 parataxis see,intestine
R4336 T7584 T7583 dobj Figure,see
R4337 T7585 T7584 nummod 1,Figure
R4338 T7586 T7583 punct ),see
R4339 T7587 T7588 advmod as,as
R434 T1158 T1157 pobj aging,during
R4340 T7588 T7576 cc as,brain
R4341 T7589 T7588 advmod well,as
R4342 T7590 T7576 conj liver,brain
R4343 T7591 T7590 punct ", ",liver
R4344 T7592 T7590 conj skin,liver
R4345 T7593 T7592 punct ", ",skin
R4346 T7594 T7592 cc and,skin
R4347 T7595 T7592 conj kidney,skin
R4348 T7596 T7597 punct (,data
R4349 T7597 T7595 meta data,kidney
R435 T1159 T1145 auxpass are,determined
R4350 T7598 T7597 amod unpublished,data
R4351 T7599 T7597 punct ),data
R4352 T7600 T7576 prep of,brain
R4353 T7601 T7602 amod late,stage
R4354 T7602 T7604 compound stage,embryos
R4355 T7603 T7602 punct -,stage
R4356 T7604 T7600 pobj embryos,of
R4357 T7605 T7571 punct .,observe
R4358 T7607 T7608 det This,pattern
R4359 T7608 T7610 nsubj pattern,is
R436 T1160 T1145 prep in,determined
R4360 T7609 T7608 compound expression,pattern
R4361 T7611 T7610 acomp consistent,is
R4362 T7612 T7611 prep with,consistent
R4363 T7613 T7614 amod previous,work
R4364 T7614 T7612 pobj work,with
R4365 T7615 T7616 dep that,observed
R4366 T7616 T7614 relcl observed,work
R4367 T7617 T7616 aux has,observed
R4368 T7618 T7616 dobj Sam68,observed
R4369 T7619 T7618 prep in,Sam68
R437 T1161 T1162 amod large,part
R4370 T7620 T7619 pobj neurons,in
R4371 T7621 T7622 punct [,52
R4372 T7622 T7619 parataxis 52,in
R4373 T7623 T7622 punct ],52
R4374 T7624 T7619 cc and,in
R4375 T7625 T7619 conj as,in
R4376 T7626 T7627 det a,substrate
R4377 T7627 T7625 pobj substrate,as
R4378 T7628 T7627 prep of,substrate
R4379 T7629 T7630 det the,kinase
R438 T1162 T1160 pobj part,in
R4380 T7630 T7628 pobj kinase,of
R4381 T7631 T7632 amod intestinal,SIK
R4382 T7632 T7630 nmod SIK,kinase
R4383 T7633 T7632 punct /,SIK
R4384 T7634 T7635 compound breast,tumor
R4385 T7635 T7632 appos tumor,SIK
R4386 T7636 T7637 punct [,43
R4387 T7637 T7627 parataxis 43,substrate
R4388 T7638 T7637 punct ],43
R4389 T7639 T7610 punct .,is
R439 T1163 T1145 prep by,determined
R4390 T7641 T7642 det The,expression
R4391 T7642 T7643 nsubjpass expression,altered
R4392 T7644 T7642 prep of,expression
R4393 T7645 T7644 pobj Sam68,of
R4394 T7646 T7643 auxpass was,altered
R4395 T7647 T7643 neg not,altered
R4396 T7648 T7643 prep in,altered
R4397 T7649 T7650 amod aging,cells
R4398 T7650 T7648 pobj cells,in
R4399 T7651 T7652 nmod bone,marrow
R440 T1164 T1163 pobj genetics,by
R4400 T7652 T7650 nmod marrow,cells
R4401 T7653 T7650 amod stromal,cells
R4402 T7654 T7648 cc or,in
R4403 T7655 T7648 conj in,in
R4404 T7656 T7657 amod senescencing,cells
R4405 T7657 T7655 pobj cells,in
R4406 T7658 T7657 nmod WI,cells
R4407 T7659 T7658 punct -,WI
R4408 T7660 T7658 nummod 38,WI
R4409 T7661 T7662 punct (,data
R441 T1165 T1166 punct [,4
R4410 T7662 T7643 meta data,altered
R4411 T7663 T7662 amod unpublished,data
R4412 T7664 T7662 punct ),data
R4413 T7665 T7643 punct .,altered
R4414 T7667 T7668 det The,presence
R4415 T7668 T7669 nsubj presence,predicted
R4416 T7670 T7668 prep of,presence
R4417 T7671 T7670 pobj Sam68,of
R4418 T7672 T7668 prep in,presence
R4419 T7673 T7672 pobj chondrocytes,in
R442 T1166 T1164 parataxis 4,genetics
R4420 T7674 T7673 punct ", ",chondrocytes
R4421 T7675 T7673 conj osteoblasts,chondrocytes
R4422 T7676 T7675 cc and,osteoblasts
R4423 T7677 T7675 conj osteoclasts,osteoblasts
R4424 T7678 T7673 prep in,chondrocytes
R4425 T7679 T7680 amod developing,cartilage
R4426 T7680 T7678 pobj cartilage,in
R4427 T7681 T7680 cc and,cartilage
R4428 T7682 T7680 conj bone,cartilage
R4429 T7683 T7684 det a,role
R443 T1167 T1168 punct –,6
R4430 T7684 T7669 dobj role,predicted
R4431 T7685 T7684 amod pivotal,role
R4432 T7686 T7684 prep for,role
R4433 T7687 T7688 det the,protein
R4434 T7688 T7686 pobj protein,for
R4435 T7689 T7684 prep in,role
R4436 T7690 T7691 amod skeletal,development
R4437 T7691 T7689 pobj development,in
R4438 T7692 T7669 punct .,predicted
R4439 T7694 T7695 det The,mice
R444 T1168 T1166 prep 6,4
R4440 T7695 T7700 nsubjpass mice,generated
R4441 T7696 T7695 nmod Sam68,mice
R4442 T7697 T7696 punct −,Sam68
R4443 T7698 T7696 punct /,Sam68
R4444 T7699 T7696 punct −,Sam68
R4445 T7701 T7700 auxpass were,generated
R4446 T7702 T7700 advcl using,generated
R4447 T7703 T7704 det a,approach
R4448 T7704 T7702 dobj approach,using
R4449 T7705 T7704 amod traditional,approach
R445 T1169 T1166 punct ],4
R4450 T7706 T7704 compound targeting,approach
R4451 T7707 T7708 advmod where,deleted
R4452 T7708 T7704 relcl deleted,approach
R4453 T7709 T7710 det the,domain
R4454 T7710 T7708 nsubjpass domain,deleted
R4455 T7711 T7710 amod functional,domain
R4456 T7712 T7710 compound KH,domain
R4457 T7713 T7708 auxpass was,deleted
R4458 T7714 T7700 cc and,generated
R4459 T7715 T7716 advmod approximately,one
R446 T1170 T1163 cc but,by
R4460 T7716 T7717 nsubj one,survived
R4461 T7717 T7700 conj survived,generated
R4462 T7718 T7716 amod third,one
R4463 T7719 T7716 prep of,one
R4464 T7720 T7721 det the,mice
R4465 T7721 T7719 pobj mice,of
R4466 T7722 T7721 nmod Sam68,mice
R4467 T7723 T7722 punct −,Sam68
R4468 T7724 T7722 punct /,Sam68
R4469 T7725 T7722 punct −,Sam68
R447 T1171 T1170 advmod also,but
R4470 T7726 T7717 prep to,survived
R4471 T7727 T7726 pobj adulthood,to
R4472 T7728 T7717 prep with,survived
R4473 T7729 T7730 det no,defects
R4474 T7730 T7728 pobj defects,with
R4475 T7731 T7730 amod apparent,defects
R4476 T7732 T7717 punct .,survived
R4477 T7734 T7735 nummod One,explanation
R4478 T7735 T7736 nsubj explanation,is
R4479 T7737 T7735 prep for,explanation
R448 T1172 T1163 conj by,by
R4480 T7738 T7739 det this,phenomenon
R4481 T7739 T7737 pobj phenomenon,for
R4482 T7740 T7741 mark that,sub-served
R4483 T7741 T7736 ccomp sub-served,is
R4484 T7742 T7743 compound Sam68,function
R4485 T7743 T7741 nsubjpass function,sub-served
R4486 T7744 T7741 auxpass is,sub-served
R4487 T7745 T7741 agent by,sub-served
R4488 T7746 T7745 pobj one,by
R4489 T7747 T7746 cc or,one
R449 T1173 T1174 amod physical,activity
R4490 T7748 T7746 conj more,one
R4491 T7749 T7746 prep of,one
R4492 T7750 T7751 poss its,members
R4493 T7751 T7749 pobj members,of
R4494 T7752 T7751 compound family,members
R4495 T7753 T7741 prep during,sub-served
R4496 T7754 T7753 pobj development,during
R4497 T7755 T7756 amod such,as
R4498 T7756 T7741 prep as,sub-served
R4499 T7757 T7756 pobj SLM,as
R450 T1174 T1172 pobj activity,by
R4500 T7758 T7757 punct -,SLM
R4501 T7759 T7757 nummod 1,SLM
R4502 T7760 T7757 cc and,SLM
R4503 T7761 T7757 conj SLM,SLM
R4504 T7762 T7761 punct -,SLM
R4505 T7763 T7761 nummod 2,SLM
R4506 T7764 T7765 punct [,47
R4507 T7765 T7736 parataxis 47,is
R4508 T7766 T7765 punct ],47
R4509 T7767 T7736 punct .,is
R451 T1175 T1172 cc and,by
R4510 T7769 T7770 advmod Alternatively,required
R4511 T7771 T7770 punct ", ",required
R4512 T7772 T7770 nsubjpass Sam68,required
R4513 T7773 T7770 auxpass is,required
R4514 T7774 T7770 neg not,required
R4515 T7775 T7770 prep during,required
R4516 T7776 T7777 amod embryonic,development
R4517 T7777 T7775 pobj development,during
R4518 T7778 T7770 punct .,required
R4519 T7780 T7781 advmod However,killed
R452 T1176 T1172 conj by,by
R4520 T7782 T7781 punct ", ",killed
R4521 T7783 T7784 nummod two,thirds
R4522 T7784 T7781 nsubjpass thirds,killed
R4523 T7785 T7784 prep of,thirds
R4524 T7786 T7787 det the,Sam68
R4525 T7787 T7785 pobj Sam68,of
R4526 T7788 T7787 punct −,Sam68
R4527 T7789 T7787 punct /,Sam68
R4528 T7790 T7787 punct −,Sam68
R4529 T7791 T7787 punct ", ",Sam68
R453 T1177 T1176 pobj alterations,by
R4530 T7792 T7787 cc but,Sam68
R4531 T7793 T7792 neg not,but
R4532 T7794 T7795 det the,Sam68
R4533 T7795 T7796 nmod Sam68,pups
R4534 T7796 T7787 conj pups,Sam68
R4535 T7797 T7795 punct +,Sam68
R4536 T7798 T7795 punct /,Sam68
R4537 T7799 T7795 punct -,Sam68
R4538 T7800 T7781 punct ", ",killed
R4539 T7801 T7781 auxpass were,killed
R454 T1178 T1177 prep in,alterations
R4540 T7802 T7781 agent by,killed
R4541 T7803 T7804 poss their,mothers
R4542 T7804 T7802 pobj mothers,by
R4543 T7805 T7804 nmod Sam68,mothers
R4544 T7806 T7805 punct +,Sam68
R4545 T7807 T7805 punct /,Sam68
R4546 T7808 T7805 punct -,Sam68
R4547 T7809 T7781 punct .,killed
R4548 T7811 T7812 det These,findings
R4549 T7812 T7813 nsubj findings,suggest
R455 T1179 T1180 det the,availability
R4550 T7814 T7815 mark that,are
R4551 T7815 T7813 ccomp are,suggest
R4552 T7816 T7817 det the,mothers
R4553 T7817 T7815 nsubj mothers,are
R4554 T7818 T7815 acomp able,are
R4555 T7819 T7820 aux to,detect
R4556 T7820 T7818 xcomp detect,able
R4557 T7821 T7822 det a,difference
R4558 T7822 T7820 dobj difference,detect
R4559 T7823 T7822 amod subtle,difference
R456 T1180 T1178 pobj availability,in
R4560 T7824 T7822 compound defect,difference
R4561 T7825 T7822 punct /,difference
R4562 T7826 T7827 dep that,can
R4563 T7827 T7822 relcl can,difference
R4564 T7828 T7827 nsubj we,can
R4565 T7829 T7827 neg not,can
R4566 T7830 T7813 punct .,suggest
R4567 T7832 T7833 amod Adult,mice
R4568 T7833 T7838 nsubj mice,live
R4569 T7834 T7833 nmod Sam68,mice
R457 T1181 T1180 cc and,availability
R4570 T7835 T7834 punct −,Sam68
R4571 T7836 T7834 punct /,Sam68
R4572 T7837 T7834 punct −,Sam68
R4573 T7839 T7840 det a,span
R4574 T7840 T7838 dobj span,live
R4575 T7841 T7840 amod normal,span
R4576 T7842 T7840 compound life,span
R4577 T7843 T7840 prep of,span
R4578 T7844 T7845 advmod approximately,two
R4579 T7845 T7846 nummod two,years
R458 T1182 T1180 conj response,availability
R4580 T7846 T7843 pobj years,of
R4581 T7847 T7838 punct .,live
R4582 T7849 T7850 det The,males
R4583 T7850 T7855 nsubj males,were
R4584 T7851 T7850 nmod Sam68,males
R4585 T7852 T7851 punct −,Sam68
R4586 T7853 T7851 punct /,Sam68
R4587 T7854 T7851 punct −,Sam68
R4588 T7856 T7855 acomp sterile,were
R4589 T7857 T7855 cc and,were
R459 T1183 T1180 prep of,availability
R4590 T7858 T7859 det the,females
R4591 T7859 T7864 nsubj females,provided
R4592 T7860 T7859 nmod Sam68,females
R4593 T7861 T7860 punct −,Sam68
R4594 T7862 T7860 punct /,Sam68
R4595 T7863 T7860 punct −,Sam68
R4596 T7864 T7855 conj provided,were
R4597 T7865 T7866 amod inadequate,care
R4598 T7866 T7864 dobj care,provided
R4599 T7867 T7864 dative to,provided
R460 T1184 T1185 compound bone,cells
R4600 T7868 T7869 poss their,young
R4601 T7869 T7867 pobj young,to
R4602 T7870 T7855 punct ", ",were
R4603 T7871 T7855 cc but,were
R4604 T7872 T7873 det the,pups
R4605 T7873 T7874 nsubjpass pups,scattered
R4606 T7874 T7855 conj scattered,were
R4607 T7875 T7874 auxpass were,scattered
R4608 T7876 T7874 neg not,scattered
R4609 T7877 T7874 cc and,scattered
R461 T1185 T1183 pobj cells,of
R4610 T7878 T7874 conj neglected,scattered
R4611 T7879 T7880 mark as,observed
R4612 T7880 T7878 advcl observed,neglected
R4613 T7881 T7880 prep with,observed
R4614 T7882 T7883 nmod FosB,mice
R4615 T7883 T7881 pobj mice,with
R4616 T7884 T7882 punct −,FosB
R4617 T7885 T7882 punct /,FosB
R4618 T7886 T7882 punct −,FosB
R4619 T7887 T7888 punct [,53
R462 T1186 T1180 prep to,availability
R4620 T7888 T7878 parataxis 53,neglected
R4621 T7889 T7888 punct ],53
R4622 T7890 T7874 punct .,scattered
R4623 T7892 T7893 compound Serum,levels
R4624 T7893 T7894 nsubj levels,decreased
R4625 T7894 T7897 ccomp decreased,decreased
R4626 T7895 T7893 prep of,levels
R4627 T7896 T7895 pobj estrogen,of
R4628 T7898 T7894 prep in,decreased
R4629 T7899 T7900 amod aging,females
R463 T1187 T1188 amod circulating,hormones
R4630 T7900 T7898 pobj females,in
R4631 T7901 T7900 nmod Sam68,females
R4632 T7902 T7901 punct −,Sam68
R4633 T7903 T7901 punct /,Sam68
R4634 T7904 T7901 punct −,Sam68
R4635 T7905 T7906 mark as,expected
R4636 T7906 T7894 advcl expected,decreased
R4637 T7907 T7897 punct ;,decreased
R4638 T7908 T7897 advmod however,decreased
R4639 T7909 T7897 punct ", ",decreased
R464 T1188 T1186 pobj hormones,to
R4640 T7910 T7911 det the,levels
R4641 T7911 T7897 nsubj levels,decreased
R4642 T7912 T7911 compound leptin,levels
R4643 T7913 T7897 prep in,decreased
R4644 T7914 T7915 amod aged,females
R4645 T7915 T7913 pobj females,in
R4646 T7916 T7915 nmod Sam68,females
R4647 T7917 T7916 punct −,Sam68
R4648 T7918 T7916 punct /,Sam68
R4649 T7919 T7916 punct −,Sam68
R465 T1189 T1190 punct [,7
R4650 T7920 T7897 punct .,decreased
R4651 T7922 T7923 det The,females
R4652 T7923 T7929 nsubj females,were
R4653 T7924 T7923 amod aged,females
R4654 T7925 T7923 nmod Sam68,females
R4655 T7926 T7925 punct −,Sam68
R4656 T7927 T7925 punct /,Sam68
R4657 T7928 T7925 punct −,Sam68
R4658 T7930 T7929 neg not,were
R4659 T7931 T7929 acomp obese,were
R466 T1190 T1188 parataxis 7,hormones
R4660 T7932 T7929 cc and,were
R4661 T7933 T7934 advmod actually,weighed
R4662 T7934 T7929 conj weighed,were
R4663 T7935 T7934 dobj less,weighed
R4664 T7936 T7935 prep than,less
R4665 T7937 T7938 det the,controls
R4666 T7938 T7936 pobj controls,than
R4667 T7939 T7938 compound littermate,controls
R4668 T7940 T7941 punct (,see
R4669 T7941 T7934 parataxis see,weighed
R467 T1191 T1192 punct –,9
R4670 T7942 T7941 dobj Table,see
R4671 T7943 T7942 nummod 2,Table
R4672 T7944 T7941 punct ),see
R4673 T7945 T7929 punct .,were
R4674 T7947 T7948 advmod Moreover,altered
R4675 T7949 T7948 punct ", ",altered
R4676 T7950 T7951 poss their,appetite
R4677 T7951 T7948 nsubjpass appetite,altered
R4678 T7952 T7948 auxpass was,altered
R4679 T7953 T7948 neg not,altered
R468 T1192 T1190 prep 9,7
R4680 T7954 T7948 prep with,altered
R4681 T7955 T7954 pobj aging,with
R4682 T7956 T7957 punct (,N.
R4683 T7957 T7948 meta N.,altered
R4684 T7958 T7957 nmod Torabi,N.
R4685 T7959 T7957 cc and,N.
R4686 T7960 T7957 conj S.,N.
R4687 T7961 T7960 nmod Richard,S.
R4688 T7962 T7960 punct ", ",S.
R4689 T7963 T7964 amod unpublished,data
R469 T1193 T1190 punct ],7
R4690 T7964 T7960 conj data,S.
R4691 T7965 T7964 punct ),data
R4692 T7966 T7948 punct ", ",altered
R4693 T7967 T7948 advcl suggesting,altered
R4694 T7968 T7969 mark that,recreating
R4695 T7969 T7967 ccomp recreating,suggesting
R4696 T7970 T7971 det the,reduction
R4697 T7971 T7969 nsubj reduction,recreating
R4698 T7972 T7971 amod observed,reduction
R4699 T7973 T7971 compound leptin,reduction
R470 T1194 T1188 cc and,hormones
R4700 T7974 T7969 aux was,recreating
R4701 T7975 T7969 neg not,recreating
R4702 T7976 T7977 dep ob,like
R4703 T7977 T7981 amod like,phenotypes
R4704 T7978 T7977 punct /,like
R4705 T7979 T7977 npadvmod ob,like
R4706 T7980 T7977 punct -,like
R4707 T7981 T7969 dobj phenotypes,recreating
R4708 T7982 T7981 acl related,phenotypes
R4709 T7983 T7982 prep to,related
R471 T1195 T1196 advmod locally,derived
R4710 T7984 T7983 pobj weight,to
R4711 T7985 T7984 punct ", ",weight
R4712 T7986 T7984 conj appetite,weight
R4713 T7987 T7986 cc and,appetite
R4714 T7988 T7989 amod female,sterility
R4715 T7989 T7986 conj sterility,appetite
R4716 T7990 T7991 punct [,54
R4717 T7991 T7948 parataxis 54,altered
R4718 T7992 T7991 punct ],54
R4719 T7993 T7948 punct .,altered
R472 T1196 T1197 amod derived,factors
R4720 T7995 T7996 det The,phenotyping
R4721 T7996 T7998 nsubj phenotyping,showed
R4722 T7997 T7996 amod skeletal,phenotyping
R4723 T7999 T7996 prep of,phenotyping
R4724 T8000 T7999 pobj cohorts,of
R4725 T8001 T8000 prep of,cohorts
R4726 T8002 T8003 nmod Sam68,mice
R4727 T8003 T8001 pobj mice,of
R4728 T8004 T8002 punct +,Sam68
R4729 T8005 T8002 punct /,Sam68
R473 T1197 T1188 conj factors,hormones
R4730 T8006 T8002 punct +,Sam68
R4731 T8007 T8002 cc and,Sam68
R4732 T8008 T8002 conj Sam68,Sam68
R4733 T8009 T8008 punct −,Sam68
R4734 T8010 T8008 punct /,Sam68
R4735 T8011 T8008 punct −,Sam68
R4736 T8012 T8013 mark that,preserved
R4737 T8013 T7998 ccomp preserved,showed
R4738 T8014 T8015 compound bone,mass
R4739 T8015 T8013 nsubjpass mass,preserved
R474 T1198 T1197 compound growth,factors
R4740 T8016 T8013 auxpass was,preserved
R4741 T8017 T8013 prep in,preserved
R4742 T8018 T8019 amod aged,mice
R4743 T8019 T8017 pobj mice,in
R4744 T8020 T8019 nmod Sam68,mice
R4745 T8021 T8020 punct −,Sam68
R4746 T8022 T8020 punct /,Sam68
R4747 T8023 T8020 punct −,Sam68
R4748 T8024 T7998 punct .,showed
R4749 T8026 T8027 amod Traditional,histology
R475 T1199 T1200 punct [,11
R4750 T8027 T8028 nsubj histology,suggested
R4751 T8029 T8027 cc and,histology
R4752 T8030 T8027 conj histomorphometry,histology
R4753 T8031 T8032 mark that,involved
R4754 T8032 T8028 ccomp involved,suggested
R4755 T8033 T8034 det the,mechanism
R4756 T8034 T8032 nsubj mechanism,involved
R4757 T8035 T8032 dobj preservation,involved
R4758 T8036 T8035 prep of,preservation
R4759 T8037 T8038 nmod osteoblast,activity
R476 T1200 T1197 parataxis 11,factors
R4760 T8038 T8036 pobj activity,of
R4761 T8039 T8037 cc and,osteoblast
R4762 T8040 T8037 conj osteoclast,osteoblast
R4763 T8041 T8028 dep .,suggested
R4764 T8043 T8044 det The,role
R4765 T8044 T8046 nsubj role,led
R4766 T8045 T8044 amod documented,role
R4767 T8047 T8044 prep of,role
R4768 T8048 T8049 det the,protein
R4769 T8049 T8047 pobj protein,of
R477 T1201 T1200 nummod 10,11
R4770 T8050 T8049 nmod Sam68,protein
R4771 T8051 T8049 amod regulatory,protein
R4772 T8052 T8049 punct ", ",protein
R4773 T8053 T8049 appos Src,protein
R4774 T8054 T8044 punct ", ",role
R4775 T8055 T8044 prep in,role
R4776 T8056 T8055 pobj osteopetrosis,in
R4777 T8057 T8046 dobj us,led
R4778 T8058 T8059 aux to,investigate
R4779 T8059 T8046 xcomp investigate,led
R478 T1202 T1200 punct ",",11
R4780 T8060 T8061 det the,morphology
R4781 T8061 T8059 dobj morphology,investigate
R4782 T8062 T8061 cc and,morphology
R4783 T8063 T8061 conj activity,morphology
R4784 T8064 T8061 prep of,morphology
R4785 T8065 T8066 nmod Sam68,osteoclasts
R4786 T8066 T8064 pobj osteoclasts,of
R4787 T8067 T8065 punct −,Sam68
R4788 T8068 T8065 punct /,Sam68
R4789 T8069 T8065 punct −,Sam68
R479 T1203 T1200 punct ],11
R4790 T8070 T8071 advmod ex,vivo
R4791 T8071 T8059 advmod vivo,investigate
R4792 T8072 T8046 punct .,led
R4793 T8074 T8075 det The,kinase
R4794 T8075 T8078 nsubjpass kinase,shown
R4795 T8076 T8075 compound Src,kinase
R4796 T8077 T8075 compound tyrosine,kinase
R4797 T8079 T8078 auxpass was,shown
R4798 T8080 T8081 aux to,play
R4799 T8081 T8078 xcomp play,shown
R480 T1204 T1145 punct .,determined
R4800 T8082 T8083 det a,role
R4801 T8083 T8081 dobj role,play
R4802 T8084 T8081 prep in,play
R4803 T8085 T8086 compound bone,remodeling
R4804 T8086 T8084 pobj remodeling,in
R4805 T8087 T8088 advmod when,died
R4806 T8088 T8081 advcl died,play
R4807 T8089 T8090 nmod Src,mice
R4808 T8090 T8088 nsubj mice,died
R4809 T8091 T8089 punct −,Src
R481 T1206 T1207 mark Whereas,provided
R4810 T8092 T8089 punct /,Src
R4811 T8093 T8089 punct −,Src
R4812 T8094 T8088 prep at,died
R4813 T8095 T8096 nummod 6,months
R4814 T8096 T8094 pobj months,at
R4815 T8097 T8096 prep of,months
R4816 T8098 T8097 pobj age,of
R4817 T8099 T8088 prep with,died
R4818 T8100 T8101 det an,phenotype
R4819 T8101 T8099 pobj phenotype,with
R482 T1207 T1213 advcl provided,known
R4820 T8102 T8101 amod osteopetrotic,phenotype
R4821 T8103 T8104 punct [,48
R4822 T8104 T8088 parataxis 48,died
R4823 T8105 T8104 punct ],48
R4824 T8106 T8088 cc and,died
R4825 T8107 T8108 det the,defect
R4826 T8108 T8109 nsubjpass defect,attributed
R4827 T8109 T8088 conj attributed,died
R4828 T8110 T8109 auxpass was,attributed
R4829 T8111 T8109 prep to,attributed
R483 T1208 T1209 amod genetic,based
R4830 T8112 T8113 amod defective,function
R4831 T8113 T8111 pobj function,to
R4832 T8114 T8113 compound osteoclast,function
R4833 T8115 T8116 punct [,55
R4834 T8116 T8109 parataxis 55,attributed
R4835 T8117 T8118 punct −,59
R4836 T8118 T8116 prep 59,55
R4837 T8119 T8116 punct ],55
R4838 T8120 T8078 punct .,shown
R4839 T8122 T8123 nsubj We,cultured
R484 T1209 T1211 amod based,studies
R4840 T8124 T8123 advmod therefore,cultured
R4841 T8125 T8126 amod mature,osteoclasts
R4842 T8126 T8123 dobj osteoclasts,cultured
R4843 T8127 T8126 acl harvested,osteoclasts
R4844 T8128 T8127 prep from,harvested
R4845 T8129 T8130 nmod Sam68,mice
R4846 T8130 T8128 pobj mice,from
R4847 T8131 T8129 punct +,Sam68
R4848 T8132 T8129 punct /,Sam68
R4849 T8133 T8129 punct +,Sam68
R485 T1210 T1209 punct -,based
R4850 T8134 T8129 cc and,Sam68
R4851 T8135 T8129 conj Sam68,Sam68
R4852 T8136 T8135 punct −,Sam68
R4853 T8137 T8135 punct /,Sam68
R4854 T8138 T8135 punct −,Sam68
R4855 T8139 T8140 advmod ex,vivo
R4856 T8140 T8123 advmod vivo,cultured
R4857 T8141 T8123 prep on,cultured
R4858 T8142 T8143 compound dentin,slices
R4859 T8143 T8141 pobj slices,on
R486 T1211 T1207 nsubj studies,provided
R4860 T8144 T8145 aux to,quantify
R4861 T8145 T8123 advcl quantify,cultured
R4862 T8146 T8147 poss their,capacity
R4863 T8147 T8145 dobj capacity,quantify
R4864 T8148 T8147 amod resorptive,capacity
R4865 T8149 T8123 punct .,cultured
R4866 T8151 T8152 det The,fact
R4867 T8152 T8153 nsubj fact,made
R4868 T8154 T8155 mark that,looked
R4869 T8155 T8152 acl looked,fact
R487 T1212 T1207 aux have,provided
R4870 T8156 T8157 nmod Sam68,osteoclasts
R4871 T8157 T8155 nsubj osteoclasts,looked
R4872 T8158 T8156 punct −,Sam68
R4873 T8159 T8156 punct /,Sam68
R4874 T8160 T8156 punct −,Sam68
R4875 T8161 T8155 cc and,looked
R4876 T8162 T8155 conj acted,looked
R4877 T8163 T8162 prep like,acted
R4878 T8164 T8165 nmod Sam68,osteoclasts
R4879 T8165 T8163 pobj osteoclasts,like
R488 T1214 T1215 amod novel,insights
R4880 T8166 T8164 punct +,Sam68
R4881 T8167 T8164 punct /,Sam68
R4882 T8168 T8164 punct +,Sam68
R4883 T8169 T8170 advmod ex,vivo
R4884 T8170 T8162 advmod vivo,acted
R4885 T8171 T8170 cc and,vivo
R4886 T8172 T8173 advmod in,vivo
R4887 T8173 T8170 conj vivo,vivo
R4888 T8174 T8175 nsubj it,unlikely
R4889 T8175 T8153 ccomp unlikely,made
R489 T1215 T1207 dobj insights,provided
R4890 T8176 T8177 mark that,was
R4891 T8177 T8175 advcl was,unlikely
R4892 T8178 T8177 nsubj this,was
R4893 T8179 T8180 det the,source
R4894 T8180 T8177 attr source,was
R4895 T8181 T8180 amod primary,source
R4896 T8182 T8180 prep of,source
R4897 T8183 T8184 det the,difference
R4898 T8184 T8182 pobj difference,of
R4899 T8185 T8184 prep in,difference
R490 T1216 T1215 prep into,insights
R4900 T8186 T8187 compound bone,metabolism
R4901 T8187 T8185 pobj metabolism,in
R4902 T8188 T8177 prep in,was
R4903 T8189 T8190 nummod 12,month
R4904 T8190 T8192 npadvmod month,old
R4905 T8191 T8190 punct -,month
R4906 T8192 T8194 amod old,mice
R4907 T8193 T8192 punct -,old
R4908 T8194 T8188 pobj mice,in
R4909 T8195 T8194 nmod Sam68,mice
R491 T1217 T1218 det the,pathways
R4910 T8196 T8195 punct −,Sam68
R4911 T8197 T8195 punct /,Sam68
R4912 T8198 T8195 punct −,Sam68
R4913 T8199 T8153 punct .,made
R4914 T8201 T8202 det The,fact
R4915 T8202 T8203 nsubj fact,suggested
R4916 T8204 T8205 mark that,were
R4917 T8205 T8202 acl were,fact
R4918 T8206 T8207 det the,levels
R4919 T8207 T8205 nsubj levels,were
R492 T1218 T1216 pobj pathways,into
R4920 T8208 T8207 compound CTX,levels
R4921 T8209 T8205 acomp lower,were
R4922 T8210 T8205 prep in,were
R4923 T8211 T8212 amod young,mice
R4924 T8212 T8210 pobj mice,in
R4925 T8213 T8211 cc and,young
R4926 T8214 T8211 conj old,young
R4927 T8215 T8212 nmod Sam68,mice
R4928 T8216 T8215 punct −,Sam68
R4929 T8217 T8215 punct /,Sam68
R493 T1219 T1220 dep that,regulate
R4930 T8218 T8215 punct −,Sam68
R4931 T8219 T8220 mark that,is
R4932 T8220 T8203 ccomp is,suggested
R4933 T8221 T8220 expl there,is
R4934 T8222 T8223 amod reduced,resorption
R4935 T8223 T8220 attr resorption,is
R4936 T8224 T8223 compound bone,resorption
R4937 T8225 T8223 prep compared,resorption
R4938 T8226 T8225 prep with,compared
R4939 T8227 T8228 amod wild,type
R494 T1220 T1218 relcl regulate,pathways
R4940 T8228 T8230 compound type,controls
R4941 T8229 T8228 punct -,type
R4942 T8230 T8226 pobj controls,with
R4943 T8231 T8230 compound littermate,controls
R4944 T8232 T8203 punct .,suggested
R4945 T8234 T8235 advmod However,occurred
R4946 T8236 T8235 punct ", ",occurred
R4947 T8237 T8238 det this,reduction
R4948 T8238 T8235 nsubj reduction,occurred
R4949 T8239 T8238 prep in,reduction
R495 T1221 T1222 compound bone,development
R4950 T8240 T8241 compound bone,resorption
R4951 T8241 T8239 pobj resorption,in
R4952 T8242 T8235 prep with,occurred
R4953 T8243 T8244 amod normal,activity
R4954 T8244 T8242 pobj activity,with
R4955 T8245 T8244 compound osteoclast,activity
R4956 T8246 T8235 punct ", ",occurred
R4957 T8247 T8248 mark as,assessed
R4958 T8248 T8235 advcl assessed,occurred
R4959 T8249 T8248 prep by,assessed
R496 T1222 T1220 dobj development,regulate
R4960 T8250 T8251 advmod in,vitro
R4961 T8251 T8252 amod vitro,culturing
R4962 T8252 T8249 pobj culturing,by
R4963 T8253 T8235 punct .,occurred
R4964 T8255 T8256 det These,observations
R4965 T8256 T8257 nsubj observations,are
R4966 T8258 T8257 acomp consistent,are
R4967 T8259 T8258 prep with,consistent
R4968 T8260 T8261 det the,mice
R4969 T8261 T8266 nsubj mice,having
R497 T1223 T1224 punct [,12
R4970 T8262 T8261 nmod Sam68,mice
R4971 T8263 T8262 punct −,Sam68
R4972 T8264 T8262 punct /,Sam68
R4973 T8265 T8262 punct −,Sam68
R4974 T8266 T8259 pcomp having,with
R4975 T8267 T8268 det a,phenotype
R4976 T8268 T8266 dobj phenotype,having
R4977 T8269 T8270 npadvmod youth,like
R4978 T8270 T8268 amod like,phenotype
R4979 T8271 T8270 punct -,like
R498 T1224 T1220 parataxis 12,regulate
R4980 T8272 T8268 compound bone,phenotype
R4981 T8273 T8257 punct .,are
R4982 T8275 T8276 advmod However,is
R4983 T8277 T8276 punct ", ",is
R4984 T8278 T8276 nsubj it,is
R4985 T8279 T8276 advmod still,is
R4986 T8280 T8276 acomp possible,is
R4987 T8281 T8276 punct ", ",is
R4988 T8282 T8283 mark that,manifest
R4989 T8283 T8276 ccomp manifest,is
R499 T1225 T1226 punct –,14
R4990 T8284 T8285 det a,impairment
R4991 T8285 T8283 nsubj impairment,manifest
R4992 T8286 T8285 amod mild,impairment
R4993 T8287 T8285 prep in,impairment
R4994 T8288 T8289 nmod Sam68,mice
R4995 T8289 T8293 compound mice,function
R4996 T8290 T8288 punct −,Sam68
R4997 T8291 T8288 punct /,Sam68
R4998 T8292 T8288 punct −,Sam68
R4999 T8293 T8287 pobj function,in
R500 T1226 T1224 prep 14,12
R5000 T8294 T8293 compound osteoclast,function
R5001 T8295 T8283 aux may,manifest
R5002 T8296 T8283 dobj itself,manifest
R5003 T8297 T8283 advmod later,manifest
R5004 T8298 T8297 prep in,later
R5005 T8299 T8298 pobj life,in
R5006 T8300 T8283 prep in,manifest
R5007 T8301 T8302 amod overall,accumulation
R5008 T8302 T8300 pobj accumulation,in
R5009 T8303 T8302 prep of,accumulation
R501 T1227 T1224 punct ],12
R5010 T8304 T8303 pobj bone,of
R5011 T8305 T8276 cc and,is
R5012 T8306 T8307 nsubj this,require
R5013 T8307 T8276 conj require,is
R5014 T8308 T8307 aux will,require
R5015 T8309 T8310 amod further,studies
R5016 T8310 T8307 dobj studies,require
R5017 T8311 T8310 amod detailed,studies
R5018 T8312 T8276 punct .,is
R5019 T8314 T8315 advmod Ex,vivo
R502 T1228 T1213 punct ", ",known
R5020 T8315 T8316 amod vivo,differentiation
R5021 T8316 T8317 nsubj differentiation,revealed
R5022 T8318 T8316 prep of,differentiation
R5023 T8319 T8320 amod primary,cells
R5024 T8320 T8318 pobj cells,of
R5025 T8321 T8322 nmod bone,marrow
R5026 T8322 T8320 nmod marrow,cells
R5027 T8323 T8320 amod stromal,cells
R5028 T8324 T8320 punct ", ",cells
R5029 T8325 T8320 acl harvested,cells
R503 T1229 T1230 advmod relatively,little
R5030 T8326 T8325 prep from,harvested
R5031 T8327 T8328 nmod Sam68,mice
R5032 T8328 T8326 pobj mice,from
R5033 T8329 T8327 punct +,Sam68
R5034 T8330 T8327 punct /,Sam68
R5035 T8331 T8327 punct +,Sam68
R5036 T8332 T8327 cc and,Sam68
R5037 T8333 T8327 conj Sam68,Sam68
R5038 T8334 T8333 punct −,Sam68
R5039 T8335 T8333 punct /,Sam68
R504 T1230 T1213 nsubjpass little,known
R5040 T8336 T8333 punct −,Sam68
R5041 T8337 T8317 punct ", ",revealed
R5042 T8338 T8317 prep down,revealed
R5043 T8339 T8340 det the,lineage
R5044 T8340 T8338 pobj lineage,down
R5045 T8341 T8340 compound osteoblast,lineage
R5046 T8342 T8343 det an,advantage
R5047 T8343 T8317 dobj advantage,revealed
R5048 T8344 T8343 amod osteogenic,advantage
R5049 T8345 T8317 prep in,revealed
R505 T1231 T1213 auxpass is,known
R5050 T8346 T8347 det the,cultures
R5051 T8347 T8345 pobj cultures,in
R5052 T8348 T8347 prep of,cultures
R5053 T8349 T8348 pobj cells,of
R5054 T8350 T8349 acl derived,cells
R5055 T8351 T8350 prep from,derived
R5056 T8352 T8353 det the,mice
R5057 T8353 T8351 pobj mice,from
R5058 T8354 T8353 nmod Sam68,mice
R5059 T8355 T8354 punct −,Sam68
R506 T1232 T1213 prep about,known
R5060 T8356 T8354 punct /,Sam68
R5061 T8357 T8354 punct −,Sam68
R5062 T8358 T8317 punct .,revealed
R5063 T8360 T8361 nsubj It,be
R5064 T8362 T8361 aux will,be
R5065 T8363 T8361 acomp important,be
R5066 T8364 T8365 aux to,demonstrate
R5067 T8365 T8361 xcomp demonstrate,be
R5068 T8366 T8367 mark that,observed
R5069 T8367 T8365 ccomp observed,demonstrate
R507 T1233 T1234 det the,etiology
R5070 T8368 T8369 det a,effect
R5071 T8369 T8367 nsubjpass effect,observed
R5072 T8370 T8369 amod similar,effect
R5073 T8407 T8392 prep into,differentiated
R5074 T8371 T8367 auxpass is,observed
R5075 T8408 T8407 pobj osteoblasts,into
R5076 T8372 T8367 advcl using,observed
R5077 T8409 T8392 prep with,differentiated
R5078 T8410 T8409 pobj BMP2,with
R5079 T8411 T8389 punct .,observed
R508 T1234 T1232 pobj etiology,about
R5080 T8413 T8414 poss Our,findings
R5081 T8373 T8374 amod primary,cells
R5082 T8414 T8415 nsubj findings,indicate
R5083 T8416 T8417 mark that,develop
R5084 T8374 T8372 dobj cells,using
R5085 T8417 T8414 advcl develop,findings
R5086 T8418 T8419 amod aged,mice
R5087 T8375 T8376 nmod bone,marrow
R5088 T8419 T8417 nsubj mice,develop
R5089 T8420 T8419 nmod Sam68,mice
R509 T1235 T1234 prep of,etiology
R5090 T8421 T8420 punct −,Sam68
R5091 T8376 T8374 nmod marrow,cells
R5092 T8422 T8420 punct /,Sam68
R5093 T8423 T8420 punct −,Sam68
R5094 T8377 T8374 amod stromal,cells
R5095 T8424 T8417 aux do,develop
R5096 T8425 T8417 neg not,develop
R5097 T8426 T8427 amod fatty,marrow
R5098 T8378 T8374 prep from,cells
R5099 T8427 T8417 dobj marrow,develop
R510 T1236 T1237 npadvmod age,related
R5100 T8428 T8427 compound bone,marrow
R5101 T8379 T8380 amod aged,mice
R5102 T8429 T8417 cc and,develop
R5103 T8430 T8431 mark that,have
R5104 T8431 T8417 conj have,develop
R5105 T8380 T8378 pobj mice,from
R5106 T8432 T8431 nsubj MEFs,have
R5107 T8433 T8432 acl derived,MEFs
R5108 T8434 T8433 prep from,derived
R5109 T8381 T8380 nmod Sam68,mice
R511 T1237 T1239 amod related,loss
R5110 T8435 T8436 nmod Sam68,mice
R5111 T8436 T8434 pobj mice,from
R5112 T8382 T8381 punct −,Sam68
R5113 T8437 T8435 punct −,Sam68
R5114 T8438 T8435 punct /,Sam68
R5115 T8383 T8381 punct /,Sam68
R5116 T8439 T8435 punct −,Sam68
R5117 T8440 T8441 amod impaired,differentiation
R5118 T8441 T8431 dobj differentiation,have
R5119 T8442 T8441 compound adipocyte,differentiation
R512 T1238 T1237 punct -,related
R5120 T8384 T8381 punct −,Sam68
R5121 T8443 T8415 punct ", ",indicate
R5122 T8444 T8445 mark that,regulates
R5123 T8445 T8415 ccomp regulates,indicate
R5124 T8385 T8361 punct .,be
R5125 T8446 T8445 nsubj Sam68,regulates
R5126 T8447 T8448 preconj both,adipocyte
R5127 T8448 T8449 nmod adipocyte,differentiation
R5128 T8387 T8388 amod Similar,findings
R5129 T8449 T8445 dobj differentiation,regulates
R513 T1239 T1235 pobj loss,of
R5130 T8450 T8448 cc and,adipocyte
R5131 T8451 T8448 conj osteoblast,adipocyte
R5132 T8452 T8415 punct .,indicate
R5133 T8454 T8455 det These,findings
R5134 T8455 T8456 nsubj findings,identify
R5135 T8388 T8389 nsubjpass findings,observed
R5136 T8390 T8389 auxpass were,observed
R5137 T8457 T8456 dobj Sam68,identify
R5138 T8458 T8456 prep as,identify
R5139 T8391 T8392 advmod when,differentiated
R514 T1240 T1239 compound bone,loss
R5140 T8459 T8460 det the,protein
R5141 T8460 T8458 pobj protein,as
R5142 T8392 T8389 advcl differentiated,observed
R5143 T8461 T8460 amod first,protein
R5144 T8462 T8463 npadvmod RNA,binding
R5145 T8463 T8460 amod binding,protein
R5146 T8393 T8394 amod embryonic,cells
R5147 T8464 T8465 aux to,regulate
R5148 T8465 T8460 advcl regulate,protein
R5149 T8394 T8392 nsubjpass cells,differentiated
R515 T1241 T1213 punct .,known
R5150 T8466 T8467 amod mesenchymal,differentiation
R5151 T8467 T8465 dobj differentiation,regulate
R5152 T8468 T8467 compound cell,differentiation
R5153 T8395 T8394 amod mesenchymal,cells
R5154 T8469 T8456 cc and,identify
R5155 T8470 T8471 det the,challenge
R5156 T8471 T8472 nsubj challenge,be
R5157 T8396 T8394 amod multipotential,cells
R5158 T8472 T8456 conj be,identify
R5159 T8473 T8472 aux will,be
R516 T1243 T1244 amod Increased,resorption
R5160 T8474 T8475 aux to,identify
R5161 T8397 T8394 compound progenitor,cells
R5162 T8475 T8472 xcomp identify,be
R5163 T8476 T8477 det the,targets
R5164 T8477 T8475 dobj targets,identify
R5165 T8398 T8394 appos C3H10T1,cells
R5166 T8478 T8477 amod specific,targets
R5167 T8479 T8477 compound RNA,targets
R5168 T8399 T8398 punct /,C3H10T1
R5169 T8480 T8481 dep that,regulates
R517 T1244 T1246 nsubjpass resorption,associated
R5170 T8400 T8398 nummod 2,C3H10T1
R5171 T8481 T8477 relcl regulates,targets
R5172 T8482 T8481 nsubj it,regulates
R5173 T8483 T8481 prep during,regulates
R5174 T8401 T8394 acl treated,cells
R5175 T8484 T8485 det this,process
R5176 T8485 T8483 pobj process,during
R5177 T8486 T8472 punct .,be
R5178 T8402 T8401 prep with,treated
R5179 T8488 T8489 det The,fact
R518 T1245 T1244 compound bone,resorption
R5180 T8489 T8490 nsubj fact,raises
R5181 T8403 T8404 compound Sam68,shRNA
R5182 T8491 T8492 mark that,altered
R5183 T8492 T8489 acl altered,fact
R5184 T8404 T8402 pobj shRNA,with
R5185 T8405 T8392 punct ", ",differentiated
R5186 T8493 T8494 compound osteoblast,function
R5187 T8406 T8392 auxpass were,differentiated
R5188 T8494 T8492 nsubjpass function,altered
R5189 T8495 T8492 auxpass is,altered
R519 T1247 T1244 prep in,resorption
R5190 T8496 T8492 prep in,altered
R5191 T8513 T8514 compound bone,mass
R5192 T8497 T8498 nmod Src,mice
R5193 T8498 T8496 pobj mice,in
R5194 T8499 T8497 punct −,Src
R5195 T8514 T8512 pobj mass,of
R5196 T8500 T8497 punct /,Src
R5197 T8501 T8497 punct −,Src
R5198 T8502 T8503 punct [,61
R5199 T8515 T8511 prep in,preservation
R520 T1248 T1249 amod elderly,men
R5200 T8503 T8492 parataxis 61,altered
R5201 T8504 T8503 nummod 60,61
R5202 T8505 T8503 punct ",",61
R5203 T8516 T8517 det the,mice
R5204 T8506 T8503 punct ],61
R5205 T8507 T8508 det the,possibility
R5206 T8508 T8490 dobj possibility,raises
R5207 T8517 T8515 pobj mice,in
R5208 T8509 T8510 mark that,linked
R5209 T8510 T8508 acl linked,possibility
R521 T1249 T1247 pobj men,in
R5210 T8511 T8510 nsubjpass preservation,linked
R5211 T8518 T8517 nmod Sam68,mice
R5212 T8512 T8511 prep of,preservation
R5213 T8519 T8518 punct −,Sam68
R5214 T8520 T8518 punct /,Sam68
R5215 T8521 T8518 punct −,Sam68
R5216 T8522 T8510 aux could,linked
R5217 T8523 T8510 auxpass be,linked
R5218 T8524 T8510 prep with,linked
R5219 T8619 T8617 amod sympathetic,pathway
R522 T1250 T1249 cc and,men
R5220 T8525 T8510 cc and,linked
R5221 T8620 T8619 punct -,sympathetic
R5222 T8621 T8615 acomp unaltered,is
R5223 T8622 T8615 prep in,is
R5224 T8526 T8510 conj regulated,linked
R5225 T8623 T8624 nmod Sam68,mice
R5226 T8527 T8526 agent by,regulated
R5227 T8624 T8622 pobj mice,in
R5228 T8528 T8526 dobj Src,regulated
R5229 T8625 T8623 punct −,Sam68
R523 T1251 T1249 conj women,men
R5230 T8626 T8623 punct /,Sam68
R5231 T8529 T8490 punct .,raises
R5232 T8627 T8623 punct −,Sam68
R5233 T8531 T8532 det The,pathway
R5234 T8628 T8615 cc and,is
R5235 T8629 T8630 mark that,explain
R5236 T8630 T8615 conj explain,is
R5237 T8631 T8632 det the,lowering
R5238 T8632 T8630 nsubj lowering,explain
R5239 T8633 T8632 prep of,lowering
R524 T1252 T1246 auxpass is,associated
R5240 T8634 T8633 pobj leptin,of
R5241 T8532 T8533 nsubjpass pathway,identified
R5242 T8635 T8630 aux may,explain
R5243 T8636 T8637 det the,levels
R5244 T8637 T8630 dobj levels,explain
R5245 T8534 T8535 prep by,regulates
R5246 T8638 T8637 amod lower,levels
R5247 T8639 T8637 prep of,levels
R5248 T8640 T8639 pobj CTX,of
R5249 T8535 T8532 relcl regulates,pathway
R525 T1253 T1246 prep with,associated
R5250 T8641 T8637 prep in,levels
R5251 T8642 T8643 det the,serum
R5252 T8643 T8641 pobj serum,in
R5253 T8536 T8534 pobj which,by
R5254 T8644 T8643 prep of,serum
R5255 T8645 T8646 nmod Sam68,mice
R5256 T8646 T8644 pobj mice,of
R5257 T8537 T8535 nsubj leptin,regulates
R5258 T8647 T8645 punct −,Sam68
R5259 T8648 T8645 punct /,Sam68
R526 T1254 T1255 det a,reduction
R5260 T8649 T8645 punct −,Sam68
R5261 T8538 T8539 compound bone,resorption
R5262 T8650 T8612 punct .,suggest
R5263 T8652 T8653 det These,findings
R5264 T8539 T8535 dobj resorption,regulates
R5265 T8653 T8654 nsubj findings,suggest
R5266 T8654 T8655 ccomp suggest,results
R5267 T8540 T8533 auxpass was,identified
R5268 T8656 T8657 mark that,regulating
R5269 T8657 T8654 ccomp regulating,suggest
R527 T1255 T1253 pobj reduction,with
R5270 T8541 T8542 aux to,involve
R5271 T8658 T8657 nsubj Sam68,regulating
R5272 T8659 T8657 aux may,regulating
R5273 T8542 T8533 xcomp involve,identified
R5274 T8660 T8657 aux be,regulating
R5275 T8661 T8662 compound bone,metabolism
R5276 T8662 T8657 dobj metabolism,regulating
R5277 T8543 T8544 det the,system
R5278 T8663 T8657 prep at,regulating
R5279 T8664 T8665 nummod two,levels
R528 T1256 T1255 prep in,reduction
R5280 T8665 T8663 pobj levels,at
R5281 T8544 T8542 dobj system,involve
R5282 T8666 T8665 amod different,levels
R5283 T8667 T8655 punct : ,results
R5284 T8668 T8669 punct (,1
R5285 T8545 T8544 amod sympathetic,system
R5286 T8669 T8655 meta 1,results
R5287 T8670 T8669 punct ),1
R5288 T8671 T8672 det the,absence
R5289 T8672 T8655 nsubj absence,results
R529 T1257 T1258 compound bone,mass
R5290 T8673 T8672 prep of,absence
R5291 T8674 T8673 pobj Sam68,of
R5292 T8546 T8544 amod nervous,system
R5293 T8675 T8655 prep in,results
R5294 T8676 T8677 amod lower,levels
R5295 T8677 T8675 pobj levels,in
R5296 T8547 T8544 acl relaying,system
R5297 T8678 T8677 compound leptin,levels
R5298 T8679 T8680 dep that,reduce
R5299 T8680 T8677 relcl reduce,levels
R530 T1258 T1256 pobj mass,in
R5300 T8548 T8547 prep to,relaying
R5301 T8681 T8680 aux may,reduce
R5302 T8682 T8683 compound bone,resorption
R5303 T8683 T8680 dobj resorption,reduce
R5304 T8549 T8550 det the,osteoblasts
R5305 T8684 T8680 prep via,reduce
R5306 T8685 T8686 det the,system
R5307 T8686 T8684 pobj system,via
R5308 T8550 T8548 pobj osteoblasts,to
R5309 T8687 T8686 amod sympathetic,system
R531 T1259 T1255 cc and,reduction
R5310 T8688 T8686 amod nervous,system
R5311 T8689 T8655 cc and,results
R5312 T8551 T8547 prep via,relaying
R5313 T8690 T8691 punct (,2
R5314 T8691 T8692 meta 2,favors
R5315 T8692 T8655 conj favors,results
R5316 T8552 T8553 det the,pathway
R5317 T8693 T8691 punct ),2
R5318 T8694 T8695 det the,absence
R5319 T8695 T8692 nsubj absence,favors
R532 T1260 T1261 det an,increase
R5320 T8553 T8551 pobj pathway,via
R5321 T8696 T8695 prep of,absence
R5322 T8697 T8696 pobj Sam68,of
R5323 T8698 T8699 nmod osteoblast,differentiation
R5324 T8554 T8555 npadvmod β,adrenergic
R5325 T8699 T8692 dobj differentiation,favors
R5326 T8700 T8698 punct ", ",osteoblast
R5327 T8701 T8702 advmod rather,than
R5328 T8555 T8553 amod adrenergic,pathway
R5329 T8702 T8698 cc than,osteoblast
R533 T1261 T1255 conj increase,reduction
R5330 T8703 T8698 conj adipocyte,osteoblast
R5331 T8556 T8555 punct -,adrenergic
R5332 T8704 T8699 punct ", ",differentiation
R5333 T8705 T8655 punct .,results
R5334 T8557 T8547 advcl leading,relaying
R5335 T8707 T8708 prep In,define
R5336 T8558 T8557 prep to,leading
R5337 T8709 T8707 pobj conclusion,In
R5338 T8710 T8708 punct ", ",define
R5339 T8711 T8712 poss our,data
R534 T1262 T1261 prep in,increase
R5340 T8559 T8560 det the,release
R5341 T8560 T8558 pobj release,to
R5342 T8712 T8708 nsubj data,define
R5343 T8713 T8714 det a,role
R5344 T8714 T8708 dobj role,define
R5345 T8561 T8560 prep of,release
R5346 T8715 T8714 amod physiologic,role
R5347 T8716 T8714 prep for,role
R5348 T8562 T8563 compound growth,factors
R5349 T8717 T8716 pobj Sam68,for
R535 T1263 T1264 amod circulating,levels
R5350 T8718 T8714 prep in,role
R5351 T8563 T8561 pobj factors,of
R5352 T8564 T8563 prep including,factors
R5353 T8719 T8720 compound bone,metabolism
R5354 T8565 T8564 pobj RANKL,including
R5355 T8720 T8718 pobj metabolism,in
R5356 T8566 T8567 dep that,causes
R5357 T8721 T8720 cc and,metabolism
R5358 T8722 T8723 nmod bone,marrow
R5359 T8567 T8563 relcl causes,factors
R536 T1264 T1262 pobj levels,in
R5360 T8723 T8724 nmod marrow,differentiation
R5361 T8724 T8720 conj differentiation,metabolism
R5362 T8568 T8569 det the,osteoclasts
R5363 T8569 T8570 nsubj osteoclasts,thrive
R5364 T8725 T8726 amod mesenchymal,cell
R5365 T8570 T8567 ccomp thrive,causes
R5366 T8726 T8724 compound cell,differentiation
R5367 T8727 T8726 compound stem,cell
R5368 T8571 T8570 aux to,thrive
R5369 T8728 T8708 punct .,define
R537 T1265 T1264 prep of,levels
R5370 T8730 T8731 det The,phenotype
R5371 T8572 T8573 punct [,8
R5372 T8731 T8733 nsubj phenotype,imply
R5373 T8732 T8731 compound bone,phenotype
R5374 T8573 T8533 parataxis 8,identified
R5375 T8734 T8731 acl observed,phenotype
R5376 T8735 T8734 prep in,observed
R5377 T8574 T8573 punct ",",8
R5378 T8736 T8737 nmod Sam68,mice
R5379 T8737 T8735 pobj mice,in
R538 T1266 T1267 compound bone,biomarkers
R5380 T8738 T8736 punct −,Sam68
R5381 T8575 T8573 appos 18,8
R5382 T8739 T8736 punct /,Sam68
R5383 T8740 T8736 punct −,Sam68
R5384 T8576 T8577 punct –,20
R5385 T8741 T8742 mark that,prevent
R5386 T8742 T8733 ccomp prevent,imply
R5387 T8743 T8742 nsubj inhibitors,prevent
R5388 T8577 T8575 prep 20,18
R5389 T8744 T8743 prep of,inhibitors
R539 T1267 T1265 pobj biomarkers,of
R5390 T8745 T8744 pobj Sam68,of
R5391 T8746 T8742 aux could,prevent
R5392 T8747 T8748 npadvmod age,related
R5393 T8748 T8750 amod related,loss
R5394 T8749 T8748 punct -,related
R5395 T8578 T8573 punct ],8
R5396 T8750 T8742 dobj loss,prevent
R5397 T8751 T8750 compound bone,loss
R5398 T8579 T8533 punct .,identified
R5399 T8752 T8733 punct .,imply
R540 T1268 T1269 punct [,15
R5400 T8754 T8755 advmod Furthermore,suggest
R5401 T8581 T8582 det The,lowering
R5402 T8756 T8755 punct ", ",suggest
R5403 T8757 T8758 det the,results
R5404 T8758 T8755 nsubj results,suggest
R5405 T8582 T8583 nsubj lowering,is
R5406 T8759 T8755 advmod also,suggest
R5407 T8760 T8761 mark that,influence
R5408 T8761 T8755 ccomp influence,suggest
R5409 T8584 T8582 prep of,lowering
R541 T1269 T1246 parataxis 15,associated
R5410 T8762 T8763 compound Sam68,levels
R5411 T8763 T8761 nsubj levels,influence
R5412 T8764 T8763 compound expression,levels
R5413 T8765 T8763 punct ", ",levels
R5414 T8585 T8586 compound leptin,levels
R5415 T8766 T8763 conj hypomorphism,levels
R5416 T8767 T8766 punct ", ",hypomorphism
R5417 T8768 T8766 cc and,hypomorphism
R5418 T8586 T8584 pobj levels,of
R5419 T8769 T8766 conj mutations,hypomorphism
R542 T1270 T1269 punct ],15
R5420 T8770 T8763 prep in,levels
R5421 T8771 T8770 pobj humans,in
R5422 T8587 T8582 prep in,lowering
R5423 T8772 T8761 aux may,influence
R5424 T8773 T8761 dobj susceptibility,influence
R5425 T8774 T8773 prep to,susceptibility
R5426 T8588 T8589 amod aged,mice
R5427 T8775 T8776 compound marrow,adipocyte
R5428 T8776 T8777 compound adipocyte,accumulation
R5429 T8777 T8774 pobj accumulation,to
R543 T1271 T1246 punct .,associated
R5430 T8589 T8587 pobj mice,in
R5431 T8778 T8777 cc and,accumulation
R5432 T8779 T8777 conj osteoporosis,accumulation
R5433 T8780 T8755 punct .,suggest
R5434 T8590 T8589 nmod Sam68,mice
R5435 T8782 T8783 poss Our,findings
R5436 T8783 T8784 nsubj findings,identify
R5437 T8591 T8590 punct −,Sam68
R5438 T8785 T8784 advmod also,identify
R5439 T8786 T8787 det a,model
R544 T1273 T1274 det These,changes
R5440 T8592 T8590 punct /,Sam68
R5441 T8787 T8784 dobj model,identify
R5442 T8788 T8787 amod new,model
R5443 T8789 T8787 compound animal,model
R5444 T8790 T8791 aux to,study
R5445 T8791 T8787 advcl study,model
R5446 T8792 T8793 amod aging,loss
R5447 T8593 T8590 punct −,Sam68
R5448 T8793 T8791 dobj loss,study
R5449 T8794 T8793 compound bone,loss
R545 T1274 T1275 nsubjpass changes,attributed
R5450 T8594 T8583 acomp consistent,is
R5451 T8795 T8784 punct .,identify
R5452 T8595 T8594 prep with,consistent
R5453 T8596 T8597 det these,mice
R5454 T8597 T8598 nsubj mice,having
R5455 T8598 T8595 pcomp having,with
R5456 T8599 T8600 det a,mass
R5457 T8600 T8598 dobj mass,having
R5458 T8601 T8600 amod high,mass
R5459 T8602 T8600 compound bone,mass
R546 T1276 T1275 aux have,attributed
R5460 T8603 T8598 prep compared,having
R5461 T8604 T8603 prep with,compared
R5462 T8605 T8606 poss their,littermates
R5463 T8606 T8604 pobj littermates,with
R5464 T8607 T8606 amod aged,littermates
R5465 T8608 T8583 punct .,is
R5466 T8610 T8611 det These,data
R5467 T8611 T8612 nsubj data,suggest
R5468 T8613 T8612 aux would,suggest
R5469 T8614 T8615 mark that,is
R547 T1277 T1275 auxpass been,attributed
R5470 T8615 T8612 advcl is,suggest
R5471 T8616 T8617 det the,pathway
R5472 T8617 T8615 nsubj pathway,is
R5473 T8618 T8619 npadvmod leptin,sympathetic
R5474 T8863 T8864 amod Histologic,analyses
R5475 T8865 T8863 punct ", ",Histologic
R5476 T8866 T8863 conj immunohistochemical,Histologic
R5477 T8867 T8866 punct ", ",immunohistochemical
R5478 T8868 T8866 cc and,immunohistochemical
R5479 T8869 T8866 conj histomorphometric,immunohistochemical
R548 T1278 T1275 advmod primarily,attributed
R5480 T8870 T8864 punct .,analyses
R5481 T8872 T8873 det All,analyses
R5482 T8873 T8874 nsubjpass analyses,performed
R5483 T8875 T8874 auxpass were,performed
R5484 T8876 T8877 advmod essentially,described
R5485 T8877 T8874 advcl described,performed
R5486 T8878 T8877 mark as,described
R5487 T8879 T8877 advmod previously,described
R5488 T8880 T8881 punct [,63
R5489 T8881 T8874 parataxis 63,performed
R549 T1279 T1275 prep to,attributed
R5490 T8882 T8881 nummod 62,63
R5491 T8883 T8881 punct ",",63
R5492 T8884 T8881 punct ],63
R5493 T8885 T8874 punct .,performed
R5494 T8887 T8888 advmod Briefly,removed
R5495 T8889 T8888 punct ", ",removed
R5496 T8890 T8891 amod embryonic,mice
R5497 T8891 T8888 nsubjpass mice,removed
R5498 T8892 T8888 auxpass were,removed
R5499 T8893 T8888 prep from,removed
R550 T1280 T1281 amod nutritional,deficits
R5500 T8894 T8895 amod timed,dams
R5501 T8895 T8893 pobj dams,from
R5502 T8896 T8895 amod pregnant,dams
R5503 T8897 T8888 prep at,removed
R5504 T8898 T8897 pobj E14.5,at
R5505 T8899 T8898 cc and,E14.5
R5506 T8900 T8898 conj E16.5,E14.5
R5507 T8901 T8888 cc and,removed
R5508 T8902 T8888 conj fixed,removed
R5509 T8903 T8902 advcl intact,fixed
R551 T1281 T1279 pobj deficits,to
R5510 T8904 T8902 prep for,fixed
R5511 T8905 T8906 nummod 36,h
R5512 T8906 T8904 pobj h,for
R5513 T8907 T8902 prep in,fixed
R5514 T8908 T8909 nummod 4,%
R5515 T8909 T8910 compound %,paraformaldehyde
R5516 T8910 T8907 pobj paraformaldehyde,in
R5517 T8911 T8902 punct ", ",fixed
R5518 T8912 T8902 conj rinsed,fixed
R5519 T8913 T8912 advmod thoroughly,rinsed
R552 T1282 T1281 acl resulting,deficits
R5520 T8914 T8912 prep in,rinsed
R5521 T8915 T8914 pobj PBS,in
R5522 T8916 T8912 punct ", ",rinsed
R5523 T8917 T8912 cc and,rinsed
R5524 T8918 T8912 conj processed,rinsed
R5525 T8919 T8918 prep for,processed
R5526 T8920 T8921 compound paraffin,embedding
R5527 T8921 T8919 pobj embedding,for
R5528 T8922 T8888 punct .,removed
R5529 T8924 T8925 amod Serial,sections
R553 T1283 T1282 prep in,resulting
R5530 T8925 T8929 nsubjpass sections,cut
R5531 T8926 T8927 nummod 4,μm
R5532 T8927 T8925 compound μm,sections
R5533 T8928 T8927 punct -,μm
R5534 T8930 T8929 auxpass were,cut
R5535 T8931 T8929 prep on,cut
R5536 T8932 T8933 det a,microtome
R5537 T8933 T8931 pobj microtome,on
R5538 T8934 T8933 amod modified,microtome
R5539 T8935 T8936 nmod Leica,RM
R554 T1284 T1283 pobj alterations,in
R5540 T8936 T8933 nmod RM,microtome
R5541 T8937 T8936 nummod 2155,RM
R5542 T8938 T8933 amod rotary,microtome
R5543 T8939 T8940 punct (,Microsystems
R5544 T8940 T8933 parataxis Microsystems,microtome
R5545 T8941 T8940 compound Leica,Microsystems
R5546 T8942 T8940 punct ", ",Microsystems
R5547 T8943 T8944 compound Richmond,Hill
R5548 T8944 T8940 npadvmod Hill,Microsystems
R5549 T8945 T8940 punct ", ",Microsystems
R555 T1285 T1284 prep in,alterations
R5550 T8946 T8940 npadvmod Ontario,Microsystems
R5551 T8947 T8940 punct ", ",Microsystems
R5552 T8948 T8940 npadvmod Canada,Microsystems
R5553 T8949 T8940 punct ),Microsystems
R5554 T8950 T8929 punct ", ",cut
R5555 T8951 T8929 conj stained,cut
R5556 T8952 T8951 prep with,stained
R5557 T8953 T8954 det a,dilution
R5558 T8954 T8952 pobj dilution,with
R5559 T8955 T8954 nummod 1,dilution
R556 T1286 T1287 det the,axis
R5560 T8956 T8957 punct :,600
R5561 T8957 T8955 prep 600,1
R5562 T8958 T8954 prep of,dilution
R5563 T8959 T8960 det the,antibody
R5564 T8960 T8958 pobj antibody,of
R5565 T8961 T8960 nmod AD1,antibody
R5566 T8962 T8960 amod anti-Sam68,antibody
R5567 T8963 T8964 punct [,46
R5568 T8964 T8951 parataxis 46,stained
R5569 T8965 T8964 punct ],46
R557 T1287 T1285 pobj axis,in
R5570 T8966 T8951 cc and,stained
R5571 T8967 T8951 conj counterstained,stained
R5572 T8968 T8967 prep with,counterstained
R5573 T8969 T8970 preconj either,green
R5574 T8970 T8968 pobj green,with
R5575 T8971 T8970 compound methyl,green
R5576 T8972 T8970 cc or,green
R5577 T8973 T8970 conj hematoxylin,green
R5578 T8974 T8929 punct .,cut
R5579 T8976 T8977 amod Adult,mice
R558 T1288 T1289 nmod parathyroid,hormone
R5580 T8977 T8978 nsubj mice,given
R5581 T8979 T8978 aux were,given
R5582 T8980 T8981 det an,injection
R5583 T8981 T8978 dobj injection,given
R5584 T8982 T8981 amod intraperitoneal,injection
R5585 T8983 T8981 prep of,injection
R5586 T8984 T8985 nummod 30,mg
R5587 T8985 T8986 nmod mg,calcein
R5588 T8986 T8983 pobj calcein,of
R5589 T8987 T8988 punct /,kg
R559 T1289 T1287 nmod hormone,axis
R5590 T8988 T8985 prep kg,mg
R5591 T8989 T8978 prep at,given
R5592 T8990 T8991 nummod 7,days
R5593 T8991 T8989 pobj days,at
R5594 T8992 T8978 cc and,given
R5595 T8993 T8994 nummod 30,mg
R5596 T8994 T8995 nmod mg,tetracycline
R5597 T8995 T8978 conj tetracycline,given
R5598 T8996 T8997 punct /,kg
R5599 T8997 T8994 prep kg,mg
R560 T1290 T1289 punct –,hormone
R5600 T8998 T8995 prep at,tetracycline
R5601 T8999 T9000 nummod 2,days
R5602 T9000 T8998 pobj days,at
R5603 T9001 T9002 amod prior,to
R5604 T9002 T9000 prep to,days
R5605 T9003 T9002 pobj sacrifice,to
R5606 T9004 T9005 aux to,label
R5607 T9005 T8978 advcl label,given
R5608 T9006 T9007 advmod actively,mineralizing
R5609 T9007 T9008 amod mineralizing,surfaces
R561 T1291 T1292 compound vitamin,D
R5610 T9008 T9005 dobj surfaces,label
R5611 T9009 T8978 punct .,given
R5612 T9011 T9012 prep After,embedded
R5613 T9013 T9014 advmod overnight,fixation
R5614 T9014 T9011 pobj fixation,After
R5615 T9015 T9014 prep in,fixation
R5616 T9016 T9017 nummod 4,%
R5617 T9017 T9018 compound %,paraformaldehyde
R5618 T9018 T9015 pobj paraformaldehyde,in
R5619 T9019 T9014 cc and,fixation
R562 T1292 T1289 appos D,hormone
R5620 T9020 T9014 conj rinsing,fixation
R5621 T9021 T9020 prep in,rinsing
R5622 T9022 T9021 pobj PBS,in
R5623 T9023 T9012 punct ", ",embedded
R5624 T9024 T9025 det the,femur
R5625 T9025 T9012 nsubjpass femur,embedded
R5626 T9026 T9025 amod left,femur
R5627 T9027 T9025 cc and,femur
R5628 T9028 T9025 conj tibia,femur
R5629 T9029 T9012 auxpass were,embedded
R563 T1293 T1294 punct [,16
R5630 T9030 T9012 prep in,embedded
R5631 T9031 T9030 pobj polymethylmethacrylate,in
R5632 T9032 T9031 punct (,polymethylmethacrylate
R5633 T9033 T9031 appos MMA,polymethylmethacrylate
R5634 T9034 T9031 punct ),polymethylmethacrylate
R5635 T9035 T9031 cc or,polymethylmethacrylate
R5636 T9036 T9037 det a,mixture
R5637 T9037 T9031 conj mixture,polymethylmethacrylate
R5638 T9038 T9037 prep of,mixture
R5639 T9039 T9040 nummod 50,%
R564 T1294 T1281 parataxis 16,deficits
R5640 T9040 T9041 compound %,MMA
R5641 T9041 T9038 pobj MMA,of
R5642 T9042 T9041 cc and,MMA
R5643 T9043 T9044 nummod 50,%
R5644 T9044 T9041 conj %,MMA
R5645 T9045 T9044 appos glycolmethacrylate,%
R5646 T9046 T9045 punct (,glycolmethacrylate
R5647 T9047 T9045 appos GMA,glycolmethacrylate
R5648 T9048 T9037 punct ),mixture
R5649 T9049 T9012 punct .,embedded
R565 T1295 T1294 punct ],16
R5650 T9051 T9052 amod Serial,sections
R5651 T9052 T9059 nsubjpass sections,left
R5652 T9053 T9054 quantmod 4,6
R5653 T9054 T9057 nummod 6,μm
R5654 T9055 T9054 punct -,6
R5655 T9056 T9054 quantmod to,6
R5656 T9057 T9052 compound μm,sections
R5657 T9058 T9057 punct -,μm
R5658 T9060 T9052 prep of,sections
R5659 T9061 T9062 npadvmod MMA,embedded
R566 T1296 T1279 punct ", ",to
R5660 T9062 T9064 amod embedded,tissues
R5661 T9063 T9062 punct -,embedded
R5662 T9064 T9060 pobj tissues,of
R5663 T9065 T9059 auxpass were,left
R5664 T9066 T9059 oprd unstained,left
R5665 T9067 T9059 cc or,left
R5666 T9068 T9059 conj stained,left
R5667 T9069 T9068 prep with,stained
R5668 T9070 T9071 compound von,Kossa
R5669 T9071 T9069 pobj Kossa,with
R567 T1297 T1279 conj to,to
R5670 T9072 T9071 cc and,Kossa
R5671 T9073 T9074 compound toluidine,blue
R5672 T9074 T9071 conj blue,Kossa
R5673 T9075 T9069 cc or,with
R5674 T9076 T9069 conj with,with
R5675 T9077 T9078 compound toluidine,blue
R5676 T9078 T9076 pobj blue,with
R5677 T9079 T9078 advmod alone,blue
R5678 T9080 T9059 punct ", ",left
R5679 T9081 T9082 mark while,stained
R568 T1298 T1299 amod gonadal,deficiency
R5680 T9082 T9059 advcl stained,left
R5681 T9083 T9084 nummod 4,μm
R5682 T9084 T9086 compound μm,sections
R5683 T9085 T9084 punct -,μm
R5684 T9086 T9082 nsubjpass sections,stained
R5685 T9087 T9088 compound MMA,GMA
R5686 T9088 T9086 compound GMA,sections
R5687 T9089 T9088 punct -,GMA
R5688 T9090 T9082 auxpass were,stained
R5689 T9091 T9082 prep for,stained
R569 T1299 T1297 pobj deficiency,to
R5690 T9092 T9093 nmod TRAP,activity
R5691 T9093 T9091 pobj activity,for
R5692 T9094 T9092 cc and,TRAP
R5693 T9095 T9092 conj ALP,TRAP
R5694 T9096 T9059 punct .,left
R5695 T9098 T9099 nsubjpass Images,captured
R5696 T9100 T9099 auxpass were,captured
R5697 T9101 T9099 advcl using,captured
R5698 T9102 T9103 det a,microscope
R5699 T9103 T9101 dobj microscope,using
R570 T1300 T1299 compound hormone,deficiency
R5700 T9104 T9105 compound Leica,DMR
R5701 T9105 T9103 compound DMR,microscope
R5702 T9106 T9107 punct (,Microsystems
R5703 T9107 T9103 parataxis Microsystems,microscope
R5704 T9108 T9107 compound Leica,Microsystems
R5705 T9109 T9107 punct ),Microsystems
R5706 T9110 T9103 acl equipped,microscope
R5707 T9111 T9110 prep with,equipped
R5708 T9112 T9113 det a,camera
R5709 T9113 T9111 pobj camera,with
R571 T1301 T1302 punct [,17
R5710 T9114 T9113 nmod Retiga,camera
R5711 T9115 T9114 nummod 1300,Retiga
R5712 T9116 T9117 punct (,Qimaging
R5713 T9117 T9113 parataxis Qimaging,camera
R5714 T9118 T9117 punct ", ",Qimaging
R5715 T9119 T9117 npadvmod Burnaby,Qimaging
R5716 T9120 T9117 punct ", ",Qimaging
R5717 T9121 T9122 compound British,Columbia
R5718 T9122 T9117 npadvmod Columbia,Qimaging
R5719 T9123 T9117 punct ", ",Qimaging
R572 T1302 T1299 parataxis 17,deficiency
R5720 T9124 T9117 npadvmod Canada,Qimaging
R5721 T9125 T9117 punct ),Qimaging
R5722 T9126 T9099 cc and,captured
R5723 T9127 T9128 det the,data
R5724 T9128 T9131 nsubj data,obtained
R5725 T9129 T9128 amod primary,data
R5726 T9130 T9128 amod histomorphometric,data
R5727 T9131 T9099 conj obtained,captured
R5728 T9132 T9131 advcl using,obtained
R5729 T9133 T9134 compound Bioquant,Prime
R573 T1303 T1302 punct ],17
R5730 T9134 T9136 compound Prime,software
R5731 T9135 T9134 compound Nova,Prime
R5732 T9136 T9132 dobj software,using
R5733 T9137 T9138 compound image,analysis
R5734 T9138 T9136 compound analysis,software
R5735 T9139 T9140 punct (,Corp
R5736 T9140 T9136 parataxis Corp,software
R5737 T9141 T9140 compound Bioquant,Corp
R5738 T9142 T9143 compound Image,Analysis
R5739 T9143 T9140 compound Analysis,Corp
R574 T1304 T1297 punct ", ",to
R5740 T9144 T9140 punct ", ",Corp
R5741 T9145 T9140 npadvmod Nashville,Corp
R5742 T9146 T9140 punct ", ",Corp
R5743 T9147 T9140 npadvmod Tennessee,Corp
R5744 T9148 T9140 punct ", ",Corp
R5745 T9149 T9150 compound United,States
R5746 T9150 T9140 npadvmod States,Corp
R5747 T9151 T9140 punct ),Corp
R5748 T9152 T9131 punct .,obtained
R5749 T9154 T9155 nsubj Nomenclature,conform
R575 T1305 T1297 conj to,to
R5750 T9156 T9154 cc and,Nomenclature
R5751 T9157 T9154 conj abbreviations,Nomenclature
R5752 T9158 T9155 prep to,conform
R5753 T9159 T9158 pobj those,to
R5754 T9160 T9159 acl recommended,those
R5755 T9161 T9160 agent by,recommended
R5756 T9162 T9163 det the,Society
R5757 T9163 T9161 pobj Society,by
R5758 T9164 T9163 compound American,Society
R5759 T9165 T9163 prep for,Society
R576 T1306 T1307 compound leptin,levels
R5760 T9166 T9167 nmod Bone,Research
R5761 T9167 T9165 pobj Research,for
R5762 T9168 T9166 cc and,Bone
R5763 T9169 T9166 conj Mineral,Bone
R5764 T9170 T9171 punct [,50
R5765 T9171 T9155 parataxis 50,conform
R5766 T9172 T9171 punct ],50
R5767 T9173 T9155 punct .,conform
R577 T1307 T1305 pobj levels,to
R5772 T9294 T9293 prep of,Generation
R5773 T9295 T9294 pobj mice,of
R5774 T9296 T9295 prep with,mice
R5775 T9297 T9298 amod targeted,disruption
R5776 T9298 T9296 pobj disruption,with
R5777 T9299 T9298 prep of,disruption
R5778 T9300 T9301 det the,gene
R5779 T9301 T9299 pobj gene,of
R578 T1308 T1307 cc and,levels
R5780 T9302 T9301 compound sam68,gene
R5781 T9303 T9293 punct .,Generation
R5782 T9305 T9306 det A,clone
R5783 T9306 T9309 nsubjpass clone,isolated
R5784 T9307 T9306 compound λ,clone
R5785 T9308 T9306 compound bacteriophage,clone
R5786 T9310 T9306 acl encompassing,clone
R5787 T9311 T9312 nmod Sam68,3
R5788 T9312 T9310 dobj 3,encompassing
R5789 T9313 T9312 nmod exons,3
R579 T1309 T1310 det the,system
R5790 T9314 T9312 prep to,3
R5791 T9315 T9314 pobj 9,to
R5792 T9316 T9309 auxpass was,isolated
R5793 T9317 T9309 prep from,isolated
R5794 T9318 T9319 det a,library
R5795 T9319 T9317 pobj library,from
R5796 T9320 T9321 nummod 129,SvJ
R5797 T9321 T9319 nmod SvJ,library
R5798 T9322 T9321 punct /,SvJ
R5799 T9323 T9319 amod genomic,library
R580 T1310 T1307 conj system,levels
R5800 T9324 T9309 advcl using,isolated
R5801 T9325 T9326 amod full,length
R5802 T9326 T9328 compound length,cDNA
R5803 T9327 T9326 punct -,length
R5804 T9328 T9324 dobj cDNA,using
R5805 T9329 T9328 compound Sam68,cDNA
R5806 T9330 T9324 prep as,using
R5807 T9331 T9332 det a,probe
R5808 T9332 T9330 pobj probe,as
R5809 T9333 T9309 punct .,isolated
R581 T1311 T1310 amod sympathetic,system
R5810 T9335 T9336 npadvmod XbaI,digested
R5811 T9336 T9338 amod digested,fragments
R5812 T9337 T9336 punct -,digested
R5813 T9338 T9342 nsubjpass fragments,subcloned
R5814 T9339 T9338 nmod Sam68,fragments
R5815 T9340 T9338 amod genomic,fragments
R5816 T9341 T9338 compound DNA,fragments
R5817 T9343 T9338 prep of,fragments
R5818 T9344 T9345 nummod 4,kb
R5819 T9345 T9343 pobj kb,of
R582 T1312 T1310 amod nervous,system
R5820 T9346 T9347 punct (,encompassing
R5821 T9347 T9345 parataxis encompassing,kb
R5822 T9348 T9347 dobj exon,encompassing
R5823 T9349 T9348 nummod 4,exon
R5824 T9350 T9348 cc and,exon
R5825 T9351 T9348 conj part,exon
R5826 T9352 T9351 prep of,part
R5827 T9353 T9352 pobj exon,of
R5828 T9354 T9353 nummod 5,exon
R5829 T9355 T9347 punct ),encompassing
R583 T1313 T1314 punct [,8
R5830 T9356 T9345 cc and,kb
R5831 T9357 T9345 conj 3kb,kb
R5832 T9358 T9359 punct (,spanning
R5833 T9359 T9357 parataxis spanning,3kb
R5834 T9360 T9359 dobj part,spanning
R5835 T9361 T9360 prep of,part
R5836 T9362 T9361 pobj exon,of
R5837 T9363 T9362 nummod 5,exon
R5838 T9364 T9360 cc and,part
R5839 T9365 T9360 conj exon,part
R584 T1314 T1310 parataxis 8,system
R5840 T9366 T9365 nummod 6,exon
R5841 T9367 T9359 punct ),spanning
R5842 T9368 T9342 auxpass were,subcloned
R5843 T9369 T9342 prep in,subcloned
R5844 T9370 T9371 compound Bluescript,SK
R5845 T9371 T9369 pobj SK,in
R5846 T9372 T9342 advcl resulting,subcloned
R5847 T9373 T9372 prep in,resulting
R5848 T9374 T9373 pobj pBS4,in
R5849 T9375 T9374 cc and,pBS4
R585 T1315 T1314 punct ",",8
R5850 T9376 T9374 conj pBS3,pBS4
R5851 T9377 T9372 punct ", ",resulting
R5852 T9378 T9372 advmod respectively,resulting
R5853 T9379 T9342 punct .,subcloned
R5854 T9381 T9382 det A,fragment
R5855 T9382 T9384 nsubjpass fragment,amplified
R5856 T9383 T9382 compound DNA,fragment
R5857 T9385 T9384 auxpass was,amplified
R5858 T9386 T9384 prep from,amplified
R5859 T9387 T9386 pobj pBS4,from
R586 T1316 T1314 appos 18,8
R5860 T9388 T9384 prep with,amplified
R5861 T9389 T9390 det the,oligonucleotides
R5862 T9390 T9388 pobj oligonucleotides,with
R5863 T9391 T9390 amod following,oligonucleotides
R5864 T9392 T9393 punct (,5
R5865 T9393 T9390 parataxis 5,oligonucleotides
R5866 T9394 T9393 punct ′,5
R5867 T9395 T9393 punct -,5
R5868 T9396 T9397 compound AAT,C
R5869 T9397 T9393 appos C,5
R587 T1317 T1318 punct –,20
R5870 T9398 T9397 compound GTC,C
R5871 T9399 T9397 compound TAG,C
R5872 T9400 T9397 compound AAA,C
R5873 T9401 T9397 compound CAA,C
R5874 T9402 T9397 compound CTC,C
R5875 T9403 T9397 compound ATA,C
R5876 T9404 T9397 compound TAC,C
R5877 T9405 T9397 compound AGA,C
R5878 T9406 T9393 punct -,5
R5879 T9407 T9393 nummod 3,5
R588 T1318 T1316 prep 20,18
R5880 T9408 T9393 punct ′,5
R5881 T9409 T9393 punct ),5
R5882 T9410 T9390 cc and,oligonucleotides
R5883 T9411 T9412 det the,primer
R5884 T9412 T9390 conj primer,oligonucleotides
R5885 T9413 T9412 amod universal,primer
R5886 T9414 T9384 punct .,amplified
R5887 T9416 T9417 det The,fragment
R5888 T9417 T9425 nsubjpass fragment,subcloned
R5889 T9418 T9419 npadvmod XbaI,digested
R589 T1319 T1314 punct ],8
R5890 T9419 T9417 amod digested,fragment
R5891 T9420 T9419 punct -,digested
R5892 T9421 T9422 nummod 1,kb
R5893 T9422 T9417 compound kb,fragment
R5894 T9423 T9422 punct -,kb
R5895 T9424 T9417 compound DNA,fragment
R5896 T9426 T9425 auxpass was,subcloned
R5897 T9427 T9425 prep in,subcloned
R5898 T9428 T9429 det the,site
R5899 T9429 T9427 pobj site,in
R590 T1320 T1305 punct ", ",to
R5900 T9430 T9429 compound XbaI,site
R5901 T9431 T9429 prep of,site
R5902 T9432 T9431 pobj pPNT,of
R5903 T9433 T9434 punct (,gift
R5904 T9434 T9432 parataxis gift,pPNT
R5905 T9435 T9434 det a,gift
R5906 T9436 T9434 prep from,gift
R5907 T9437 T9438 compound Andrew,Karaplis
R5908 T9438 T9436 pobj Karaplis,from
R5909 T9439 T9438 punct ", ",Karaplis
R591 T1321 T1305 cc and,to
R5910 T9440 T9441 compound McGill,University
R5911 T9441 T9438 npadvmod University,Karaplis
R5912 T9442 T9438 punct ", ",Karaplis
R5913 T9443 T9438 npadvmod Montréal,Karaplis
R5914 T9444 T9438 punct ", ",Karaplis
R5915 T9445 T9438 npadvmod Quebec,Karaplis
R5916 T9446 T9438 punct ", ",Karaplis
R5917 T9447 T9438 npadvmod Canada,Karaplis
R5918 T9448 T9434 punct ),gift
R5919 T9449 T9425 punct .,subcloned
R592 T1322 T1305 conj to,to
R5920 T9451 T9452 det The,fragment
R5921 T9452 T9456 nsubjpass fragment,amplified
R5922 T9453 T9454 nummod 3,kb
R5923 T9454 T9452 compound kb,fragment
R5924 T9455 T9454 punct -,kb
R5925 T9457 T9452 prep from,fragment
R5926 T9458 T9457 pobj pBS3,from
R5927 T9459 T9456 auxpass was,amplified
R5928 T9460 T9456 prep by,amplified
R5929 T9461 T9460 pobj PCR,by
R593 T1323 T1322 pobj alterations,to
R5930 T9462 T9461 prep with,PCR
R5931 T9463 T9462 punct (,with
R5932 T9464 T9462 pobj 5,with
R5933 T9465 T9464 punct ′,5
R5934 T9466 T9464 punct -,5
R5935 T9467 T9468 compound GGG,G
R5936 T9468 T9464 appos G,5
R5937 T9469 T9468 compound ATG,G
R5938 T9470 T9468 compound CGG,G
R5939 T9471 T9468 compound CCG,G
R594 T1324 T1323 prep in,alterations
R5940 T9472 T9468 compound CTC,G
R5941 T9473 T9468 compound TAG,G
R5942 T9474 T9468 compound AAT,G
R5943 T9475 T9468 compound TGT,G
R5944 T9476 T9468 compound CCT,G
R5945 T9477 T9468 compound ACT,G
R5946 T9478 T9468 compound TGA,G
R5947 T9479 T9468 compound ACG,G
R5948 T9480 T9464 punct -,5
R5949 T9481 T9464 nummod 3,5
R595 T1325 T1326 compound bone,cell
R5950 T9482 T9464 punct ',5
R5951 T9483 T9464 punct ),5
R5952 T9484 T9464 cc and,5
R5953 T9485 T9486 punct (,5
R5954 T9486 T9464 conj 5,5
R5955 T9487 T9486 punct ′,5
R5956 T9488 T9486 punct -,5
R5957 T9489 T9490 compound CGG,A
R5958 T9490 T9486 appos A,5
R5959 T9491 T9490 compound TGG,A
R596 T1326 T1327 compound cell,apoptosis
R5960 T9492 T9490 compound CGG,A
R5961 T9493 T9490 compound CCG,A
R5962 T9494 T9490 compound CTG,A
R5963 T9495 T9490 compound TCG,A
R5964 T9496 T9490 compound ACC,A
R5965 T9497 T9490 compound TGA,A
R5966 T9498 T9490 compound GTA,A
R5967 T9499 T9490 compound ACA,A
R5968 T9500 T9490 compound TTT,A
R5969 T9501 T9490 compound CTT,A
R597 T1327 T1324 pobj apoptosis,in
R5970 T9502 T9486 punct -,5
R5971 T9503 T9486 nummod 3,5
R5972 T9504 T9486 punct ′,5
R5973 T9505 T9456 punct ),amplified
R5974 T9506 T9456 cc and,amplified
R5975 T9507 T9456 conj subcloned,amplified
R5976 T9508 T9507 prep in,subcloned
R5977 T9509 T9510 det the,site
R5978 T9510 T9508 pobj site,in
R5979 T9511 T9510 compound NotI,site
R598 T1328 T1329 punct [,21
R5980 T9512 T9510 prep of,site
R5981 T9513 T9512 pobj pPNT,of
R5982 T9514 T9456 punct .,amplified
R5983 T9516 T9517 det The,vector
R5984 T9517 T9519 nsubj vector,replaces
R5985 T9518 T9517 compound targeting,vector
R5986 T9520 T9521 compound pPNT,Sam68
R5987 T9521 T9517 appos Sam68,vector
R5988 T9522 T9521 punct -,Sam68
R5989 T9523 T9519 dobj exon,replaces
R599 T1329 T1323 parataxis 21,alterations
R5990 T9524 T9523 nummod 4,exon
R5991 T9525 T9523 cc and,exon
R5992 T9526 T9523 conj part,exon
R5993 T9527 T9526 prep of,part
R5994 T9528 T9527 pobj exon,of
R5995 T9529 T9528 nummod 5,exon
R5996 T9530 T9519 prep with,replaces
R5997 T9531 T9532 det a,cassette
R5998 T9532 T9530 pobj cassette,with
R5999 T9533 T9534 npadvmod neomycin,resistant
R600 T1330 T1329 punct ],21
R6000 T9534 T9532 amod resistant,cassette
R6001 T9535 T9534 punct -,resistant
R6002 T9536 T9532 compound gene,cassette
R6003 T9537 T9519 punct .,replaces
R6004 T9539 T9540 det An,site
R6005 T9540 T9542 nsubjpass site,introduced
R6006 T9541 T9540 compound SalI,site
R6007 T9543 T9542 auxpass was,introduced
R6008 T9544 T9542 prep at,introduced
R6009 T9545 T9546 det the,end
R601 T1331 T1275 punct .,attributed
R6010 T9546 T9544 pobj end,at
R6011 T9547 T9546 nummod 3,end
R6012 T9548 T9547 punct ′,3
R6013 T9549 T9546 prep of,end
R6014 T9550 T9551 det the,fragment
R6015 T9551 T9549 pobj fragment,of
R6016 T9552 T9553 nummod 3,kb
R6017 T9553 T9551 compound kb,fragment
R6018 T9554 T9553 punct -,kb
R6019 T9555 T9551 compound DNA,fragment
R602 T1333 T1334 compound Bone,loss
R6020 T9556 T9542 cc and,introduced
R6021 T9557 T9558 auxpass was,used
R6022 T9558 T9542 conj used,introduced
R6023 T9559 T9560 aux to,linearize
R6024 T9560 T9558 advcl linearize,used
R6025 T9561 T9562 det the,plasmid
R6026 T9562 T9560 dobj plasmid,linearize
R6027 T9563 T9560 prep for,linearize
R6028 T9564 T9563 pobj electroporation,for
R6029 T9565 T9564 prep into,electroporation
R603 T1334 T1335 nsubjpass loss,attributed
R6030 T9566 T9567 amod embryonic,stem
R6031 T9567 T9568 nmod stem,cells
R6032 T9568 T9565 pobj cells,into
R6033 T9569 T9567 punct (,stem
R6034 T9570 T9567 appos ES,stem
R6035 T9571 T9568 punct ),cells
R6036 T9572 T9542 punct .,introduced
R6037 T9574 T9575 advmod Approximately,"1,000"
R6038 T9575 T9576 nummod "1,000",colonies
R6039 T9576 T9578 nsubjpass colonies,screened
R604 T1336 T1334 prep in,loss
R6040 T9577 T9576 compound ES,colonies
R6041 T9579 T9578 auxpass were,screened
R6042 T9580 T9578 cc and,screened
R6043 T9581 T9582 nummod two,clones
R6044 T9582 T9583 nsubjpass clones,identified
R6045 T9583 T9578 conj identified,screened
R6046 T9584 T9583 auxpass were,identified
R6047 T9585 T9586 dep that,contained
R6048 T9586 T9583 ccomp contained,identified
R6049 T9587 T9588 det the,Sam68
R605 T1337 T1338 det the,elderly
R6050 T9588 T9589 compound Sam68,allele
R6051 T9589 T9586 dobj allele,contained
R6052 T9590 T9589 compound mutant,allele
R6053 T9591 T9583 punct ", ",identified
R6054 T9592 T9593 mark as,determined
R6055 T9593 T9583 advcl determined,identified
R6056 T9594 T9593 prep by,determined
R6057 T9595 T9596 compound Southern,blotting
R6058 T9596 T9594 pobj blotting,by
R6059 T9597 T9583 punct .,identified
R606 T1338 T1336 pobj elderly,in
R6060 T9599 T9600 amod Targeted,cells
R6061 T9600 T9602 nsubjpass cells,injected
R6062 T9601 T9600 compound ES,cells
R6063 T9603 T9602 auxpass were,injected
R6064 T9604 T9602 prep into,injected
R6065 T9605 T9606 nummod 3.5,day
R6066 T9606 T9608 npadvmod day,old
R6067 T9607 T9606 punct -,day
R6068 T9608 T9610 amod old,blastocysts
R6069 T9609 T9608 punct -,old
R607 T1339 T1335 aux has,attributed
R6070 T9610 T9604 pobj blastocysts,into
R6071 T9611 T9612 compound BALB,c
R6072 T9612 T9610 compound c,blastocysts
R6073 T9613 T9612 punct /,c
R6074 T9614 T9602 cc and,injected
R6075 T9615 T9616 auxpass were,transferred
R6076 T9616 T9602 conj transferred,injected
R6077 T9617 T9616 prep into,transferred
R6078 T9618 T9619 nmod CD,mothers
R6079 T9619 T9617 pobj mothers,into
R608 T1340 T1335 advmod also,attributed
R6080 T9620 T9618 punct -,CD
R6081 T9621 T9618 nummod 1,CD
R6082 T9622 T9619 compound foster,mothers
R6083 T9623 T9602 punct ", ",injected
R6084 T9624 T9602 cc and,injected
R6085 T9625 T9626 nsubjpass animals,mated
R6086 T9626 T9602 conj mated,injected
R6087 T9627 T9625 acl classified,animals
R6088 T9628 T9627 prep as,classified
R6089 T9629 T9628 pobj chimeras,as
R609 T1341 T1335 auxpass been,attributed
R6090 T9630 T9627 prep by,classified
R6091 T9631 T9632 compound coat,color
R6092 T9632 T9630 pobj color,by
R6093 T9633 T9626 auxpass were,mated
R6094 T9634 T9626 prep with,mated
R6095 T9635 T9636 compound BALB,c
R6096 T9636 T9638 compound c,mice
R6097 T9637 T9636 punct /,c
R6098 T9638 T9634 pobj mice,with
R6099 T9639 T9626 punct .,mated
R610 T1342 T1335 prep to,attributed
R6100 T9641 T9642 compound Germ,line
R6101 T9642 T9643 compound line,transmission
R6102 T9643 T9644 nsubjpass transmission,achieved
R6103 T9645 T9644 auxpass was,achieved
R6104 T9646 T9644 cc and,achieved
R6105 T9647 T9648 det the,mice
R6106 T9648 T9649 nsubjpass mice,maintained
R6107 T9649 T9644 conj maintained,achieved
R6108 T9650 T9649 auxpass were,maintained
R6109 T9651 T9649 prep in,maintained
R611 T1343 T1342 pobj alterations,to
R6110 T9652 T9653 nmod C57BL,background
R6111 T9653 T9651 pobj background,in
R6112 T9654 T9652 punct /,C57BL
R6113 T9655 T9652 nummod 6,C57BL
R6114 T9656 T9649 punct .,maintained
R6115 T9658 T9659 det The,mice
R6116 T9659 T9660 nsubj mice,represent
R6117 T9660 T9665 ccomp represent,maintained
R6118 T9661 T9659 acl used,mice
R6119 T9662 T9661 prep for,used
R612 T1344 T1343 prep in,alterations
R6120 T9663 T9664 det this,study
R6121 T9664 T9662 pobj study,for
R6122 T9666 T9660 dobj mice,represent
R6123 T9667 T9668 dep that,backcrossed
R6124 T9668 T9666 relcl backcrossed,mice
R6125 T9669 T9668 auxpass were,backcrossed
R6126 T9670 T9668 prep in,backcrossed
R6127 T9671 T9670 pobj C57BL,in
R6128 T9672 T9671 punct /,C57BL
R6129 T9673 T9671 nummod 6,C57BL
R613 T1345 T1346 det the,response
R6130 T9674 T9675 quantmod between,three
R6131 T9675 T9676 nummod three,generations
R6132 T9676 T9668 npadvmod generations,backcrossed
R6133 T9677 T9675 cc and,three
R6134 T9678 T9675 conj eight,three
R6135 T9679 T9665 punct ;,maintained
R6136 T9680 T9665 prep in,maintained
R6137 T9681 T9680 pobj addition,in
R6138 T9682 T9665 punct ", ",maintained
R6139 T9683 T9665 nsubj we,maintained
R614 T1346 T1344 pobj response,in
R6140 T9684 T9685 det the,mice
R6141 T9685 T9665 dobj mice,maintained
R6142 T9686 T9685 prep in,mice
R6143 T9687 T9688 det the,strain
R6144 T9688 T9686 pobj strain,in
R6145 T9689 T9690 nummod 129,SvJ
R6146 T9690 T9688 compound SvJ,strain
R6147 T9691 T9690 punct /,SvJ
R6148 T9692 T9665 cc and,maintained
R6149 T9693 T9665 conj observed,maintained
R615 T1347 T1346 prep of,response
R6150 T9694 T9695 det a,phenotype
R6151 T9695 T9693 dobj phenotype,observed
R6152 T9696 T9695 amod similar,phenotype
R6153 T9697 T9665 punct .,maintained
R6154 T9733 T9732 cc and,Genotyping
R6155 T9734 T9735 compound immunoblot,analyses
R6156 T9735 T9732 conj analyses,Genotyping
R6157 T9736 T9735 punct .,analyses
R6158 T9738 T9739 det All,procedures
R6159 T9739 T9741 nsubjpass procedures,performed
R616 T1348 T1349 nmod bone,marrow
R6160 T9740 T9739 compound mouse,procedures
R6161 T9742 T9741 auxpass were,performed
R6162 T9743 T9741 prep in,performed
R6163 T9744 T9743 pobj accordance,in
R6164 T9745 T9744 prep with,accordance
R6165 T9746 T9747 compound McGill,University
R6166 T9747 T9748 compound University,guidelines
R6167 T9748 T9745 pobj guidelines,with
R6168 T9749 T9748 punct ", ",guidelines
R6169 T9750 T9751 dep which,set
R617 T1349 T1350 nmod marrow,cells
R6170 T9751 T9748 relcl set,guidelines
R6171 T9752 T9751 auxpass are,set
R6172 T9753 T9751 agent by,set
R6173 T9754 T9755 det the,Council
R6174 T9755 T9753 pobj Council,by
R6175 T9756 T9755 compound Canadian,Council
R6176 T9757 T9755 prep on,Council
R6177 T9758 T9759 compound Animal,Care
R6178 T9759 T9757 pobj Care,on
R6179 T9760 T9741 punct .,performed
R618 T1350 T1347 pobj cells,of
R6180 T9762 T9763 amod Genomic,DNA
R6181 T9763 T9764 nsubjpass DNA,isolated
R6182 T9765 T9764 auxpass was,isolated
R6183 T9766 T9764 prep from,isolated
R6184 T9767 T9768 compound tail,biopsies
R6185 T9768 T9766 pobj biopsies,from
R6186 T9769 T9764 cc and,isolated
R6187 T9770 T9764 conj analyzed,isolated
R6188 T9771 T9770 prep by,analyzed
R6189 T9772 T9773 compound Southern,blotting
R619 T1351 T1350 amod stromal,cells
R6190 T9773 T9771 pobj blotting,by
R6191 T9774 T9773 cc and,blotting
R6192 T9775 T9776 amod genomic,analysis
R6193 T9776 T9773 conj analysis,blotting
R6194 T9777 T9776 compound PCR,analysis
R6195 T9778 T9764 punct .,isolated
R6196 T9780 T9781 det The,fragment
R6197 T9781 T9783 nsubjpass fragment,amplified
R6198 T9782 T9781 compound DNA,fragment
R6199 T9784 T9781 acl utilized,fragment
R620 T1352 T1346 prep to,response
R6200 T9785 T9784 prep as,utilized
R6201 T9786 T9787 det the,probe
R6202 T9787 T9785 pobj probe,as
R6203 T9788 T9787 prep for,probe
R6204 T9789 T9790 det the,analysis
R6205 T9790 T9788 pobj analysis,for
R6206 T9791 T9792 compound Southern,blotting
R6207 T9792 T9790 compound blotting,analysis
R6208 T9793 T9783 auxpass was,amplified
R6209 T9794 T9783 prep with,amplified
R621 T1353 T1354 poss their,microenvironment
R6210 T9795 T9796 det the,oligonucleotides
R6211 T9796 T9794 pobj oligonucleotides,with
R6212 T9797 T9796 amod following,oligonucleotides
R6213 T9798 T9796 nummod two,oligonucleotides
R6214 T9799 T9796 punct (,oligonucleotides
R6215 T9800 T9796 appos 5,oligonucleotides
R6216 T9801 T9800 punct ′,5
R6217 T9802 T9800 punct -,5
R6218 T9803 T9804 compound AAG,T
R6219 T9804 T9800 appos T,5
R622 T1354 T1352 pobj microenvironment,to
R6220 T9805 T9804 compound CCT,T
R6221 T9806 T9804 compound TTA,T
R6222 T9807 T9804 compound CTG,T
R6223 T9808 T9804 compound GTT,T
R6224 T9809 T9804 compound GTG,T
R6225 T9810 T9800 punct -,5
R6226 T9811 T9800 nummod 3,5
R6227 T9812 T9800 punct ′,5
R6228 T9813 T9800 punct ),5
R6229 T9814 T9800 cc and,5
R623 T1355 T1356 dep that,favors
R6230 T9815 T9816 punct (,5
R6231 T9816 T9800 conj 5,5
R6232 T9817 T9816 punct ′,5
R6233 T9818 T9816 punct -,5
R6234 T9819 T9820 compound CTT,CT
R6235 T9820 T9816 appos CT,5
R6236 T9821 T9820 compound GAA,CT
R6237 T9822 T9820 compound ACG,CT
R6238 T9823 T9820 compound CAC,CT
R6239 T9824 T9820 compound CGT,CT
R624 T1356 T1346 relcl favors,response
R6240 T9825 T9820 compound AGG,CT
R6241 T9826 T9816 punct -,5
R6242 T9827 T9816 nummod 3,5
R6243 T9828 T9816 punct ′,5
R6244 T9829 T9783 punct ),amplified
R6245 T9830 T9783 punct .,amplified
R6246 T9832 T9833 det The,allele
R6247 T9833 T9838 nsubjpass allele,identified
R6248 T9834 T9835 amod wild,type
R6249 T9835 T9833 compound type,allele
R625 T1357 T1356 dobj differentiation,favors
R6250 T9836 T9835 punct -,type
R6251 T9837 T9833 compound sam68,allele
R6252 T9839 T9838 auxpass was,identified
R6253 T9840 T9838 prep by,identified
R6254 T9841 T9842 amod genomic,PCR
R6255 T9842 T9840 pobj PCR,by
R6256 T9843 T9838 advcl using,identified
R6257 T9844 T9845 det the,oligonucleotides
R6258 T9845 T9843 dobj oligonucleotides,using
R6259 T9846 T9845 amod following,oligonucleotides
R626 T1358 T1357 prep down,differentiation
R6260 T9847 T9845 appos 5,oligonucleotides
R6261 T9848 T9847 punct ′,5
R6262 T9849 T9847 punct -,5
R6263 T9850 T9851 compound AAA,AG
R6264 T9851 T9847 appos AG,5
R6265 T9852 T9851 compound TCC,AG
R6266 T9853 T9851 compound TAA,AG
R6267 T9854 T9851 compound CCC,AG
R6268 T9855 T9851 compound TCC,AG
R6269 T9856 T9851 compound TCA,AG
R627 T1359 T1360 det the,lineage
R6270 T9857 T9851 compound GTC,AG
R6271 T9858 T9847 punct -,5
R6272 T9859 T9847 nummod 3,5
R6273 T9860 T9847 punct ′,5
R6274 T9861 T9847 cc and,5
R6275 T9862 T9847 conj 5,5
R6276 T9863 T9862 punct ′,5
R6277 T9864 T9862 punct -,5
R6278 T9865 T9866 compound GAT,CAG
R6279 T9866 T9862 appos CAG,5
R628 T1360 T1358 pobj lineage,down
R6280 T9867 T9866 compound ATG,CAG
R6281 T9868 T9866 compound ATG,CAG
R6282 T9869 T9866 compound GAT,CAG
R6283 T9870 T9866 compound GAT,CAG
R6284 T9871 T9866 compound ATC,CAG
R6285 T9872 T9866 compound TGT,CAG
R6286 T9873 T9862 punct -,5
R6287 T9874 T9862 nummod 3,5
R6288 T9875 T9862 punct ′,5
R6289 T9876 T9838 punct .,identified
R629 T1361 T1360 compound adipocyte,lineage
R6290 T9878 T9879 det The,allele
R6291 T9879 T9883 nsubjpass allele,identified
R6292 T9880 T9881 npadvmod sam68,targeted
R6293 T9881 T9879 amod targeted,allele
R6294 T9882 T9881 punct -,targeted
R6295 T9884 T9883 auxpass was,identified
R6296 T9885 T9883 prep by,identified
R6297 T9886 T9887 amod genomic,PCR
R6298 T9887 T9885 pobj PCR,by
R6299 T9888 T9883 advcl using,identified
R630 T1362 T1363 advmod rather,than
R6300 T9889 T9890 det the,oligonucleotides
R6301 T9890 T9888 dobj oligonucleotides,using
R6302 T9891 T9890 amod following,oligonucleotides
R6303 T9892 T9890 appos 5,oligonucleotides
R6304 T9893 T9892 punct ′,5
R6305 T9894 T9892 punct -,5
R6306 T9895 T9896 compound CTT,TCG
R6307 T9896 T9892 appos TCG,5
R6308 T9897 T9896 compound GGG,TCG
R6309 T9898 T9896 compound TGG,TCG
R631 T1363 T1360 cc than,lineage
R6310 T9899 T9896 compound AGA,TCG
R6311 T9900 T9896 compound GGC,TCG
R6312 T9901 T9896 compound TAT,TCG
R6313 T9902 T9892 punct -,5
R6314 T9903 T9892 nummod 3,5
R6315 T9904 T9892 punct ′,5
R6316 T9905 T9892 cc and,5
R6317 T9906 T9892 conj 5,5
R6318 T9907 T9906 punct ′,5
R6319 T9908 T9906 punct -,5
R632 T1364 T1365 det the,lineage
R6320 T9909 T9910 compound GTC,C
R6321 T9910 T9906 appos C,5
R6322 T9911 T9910 compound GGG,C
R6323 T9912 T9910 compound CAT,C
R6324 T9913 T9910 compound GCG,C
R6325 T9914 T9910 compound CGC,C
R6326 T9915 T9910 compound CTT,C
R6327 T9916 T9910 compound GAG,C
R6328 T9917 T9906 punct -,5
R6329 T9918 T9906 nummod 3,5
R633 T1365 T1360 conj lineage,lineage
R6330 T9919 T9906 punct ′,5
R6331 T9920 T9883 punct .,identified
R6332 T9979 T9978 cc and,Radiology
R6333 T9980 T9981 compound serum,biochemistry
R6334 T9981 T9978 conj biochemistry,Radiology
R6335 T9982 T9978 prep on,Radiology
R6336 T9983 T9984 nmod Sam68,mice
R6337 T9984 T9982 pobj mice,on
R6338 T9985 T9983 punct +,Sam68
R6339 T9986 T9983 punct /,Sam68
R634 T1366 T1365 compound osteoblast,lineage
R6340 T9987 T9983 punct +,Sam68
R6341 T9988 T9983 cc and,Sam68
R6342 T9989 T9983 conj Sam68,Sam68
R6343 T9990 T9989 punct −,Sam68
R6344 T9991 T9989 punct /,Sam68
R6345 T9992 T9989 punct −,Sam68
R6346 T9993 T9978 punct .,Radiology
R6347 T9995 T9996 nsubjpass Radiography,performed
R6348 T9997 T9995 punct ", ",Radiography
R6349 T9998 T9995 conj BMD,Radiography
R635 T1367 T1368 punct [,22
R6350 T9999 T9998 punct ", ",BMD
R6351 T10000 T9998 cc and,BMD
R6352 T10001 T9998 conj micro-CT,BMD
R6353 T10002 T9996 auxpass were,performed
R6354 T10003 T10004 advmod essentially,described
R6355 T10004 T9996 advcl described,performed
R6356 T10005 T10004 mark as,described
R6357 T10006 T10004 advmod previously,described
R6358 T10007 T10008 punct [,63
R6359 T10008 T9996 parataxis 63,performed
R636 T1368 T1335 parataxis 22,attributed
R6360 T10009 T10008 punct ],63
R6361 T10010 T9996 punct .,performed
R6362 T10012 T10013 nsubjpass Mice,administered
R6363 T10014 T10013 auxpass were,administered
R6364 T10015 T10016 det a,dose
R6365 T10016 T10013 dobj dose,administered
R6366 T10017 T10016 amod lethal,dose
R6367 T10018 T10016 prep of,dose
R6368 T10019 T10018 pobj anesthetic,of
R6369 T10020 T10013 prep at,administered
R637 T1369 T1368 punct ],22
R6370 T10021 T10022 det the,times
R6371 T10022 T10020 pobj times,at
R6372 T10023 T10022 amod indicated,times
R6373 T10024 T10013 punct ", ",administered
R6374 T10025 T10013 conj exsanguinated,administered
R6375 T10026 T10025 punct ", ",exsanguinated
R6376 T10027 T10025 cc and,exsanguinated
R6377 T10028 T10025 conj imaged,exsanguinated
R6378 T10029 T10028 advcl using,imaged
R6379 T10030 T10031 det a,MX20
R638 T1370 T1335 punct .,attributed
R6380 T10031 T10029 dobj MX20,using
R6381 T10032 T10031 compound Faxitron,MX20
R6382 T10033 T10031 acl equipped,MX20
R6383 T10034 T10033 prep with,equipped
R6384 T10035 T10036 det an,system
R6385 T10036 T10034 pobj system,with
R6386 T10037 T10036 nmod FPX,system
R6387 T10038 T10037 punct -,FPX
R6388 T10039 T10037 nummod 2,FPX
R6389 T10040 T10036 compound Imaging,system
R639 T1372 T1373 nsubjpass Aging,associated
R6390 T10041 T10042 punct (,Medoptics
R6391 T10042 T10031 parataxis Medoptics,MX20
R6392 T10043 T10042 compound Dalsa,Medoptics
R6393 T10044 T10042 punct ", ",Medoptics
R6394 T10045 T10042 npadvmod Waterloo,Medoptics
R6395 T10046 T10042 punct ", ",Medoptics
R6396 T10047 T10042 npadvmod Ontario,Medoptics
R6397 T10048 T10042 punct ", ",Medoptics
R6398 T10049 T10042 npadvmod Canada,Medoptics
R6399 T10050 T10042 punct ),Medoptics
R640 T1374 T1373 aux has,associated
R6400 T10051 T10013 punct .,administered
R6401 T10053 T10054 compound Body,fat
R6402 T10054 T10055 nsubjpass fat,evaluated
R6403 T10056 T10054 punct ", ",fat
R6404 T10057 T10054 conj BMC,fat
R6405 T10058 T10057 punct ", ",BMC
R6406 T10059 T10057 cc and,BMC
R6407 T10060 T10057 conj BMD,BMC
R6408 T10061 T10055 auxpass were,evaluated
R6409 T10062 T10055 advcl using,evaluated
R641 T1375 T1373 advmod long,associated
R6410 T10063 T10064 det a,PixiMUS
R6411 T10064 T10062 dobj PixiMUS,using
R6412 T10065 T10064 compound Lunar,PixiMUS
R6413 T10066 T10064 nummod 1.46,PixiMUS
R6414 T10067 T10068 punct (,Lunar
R6415 T10068 T10064 parataxis Lunar,PixiMUS
R6416 T10069 T10068 compound GE,Lunar
R6417 T10070 T10068 punct -,Lunar
R6418 T10071 T10068 punct ", ",Lunar
R6419 T10072 T10068 npadvmod Madison,Lunar
R642 T1376 T1373 auxpass been,associated
R6420 T10073 T10068 punct ", ",Lunar
R6421 T10074 T10068 npadvmod Wisconsin,Lunar
R6422 T10075 T10068 punct ", ",Lunar
R6423 T10076 T10077 compound United,States
R6424 T10077 T10068 npadvmod States,Lunar
R6425 T10078 T10068 punct ),Lunar
R6426 T10079 T10055 punct .,evaluated
R6427 T10081 T10082 amod Morphometric,parameters
R6428 T10082 T10083 nsubjpass parameters,determined
R6429 T10084 T10083 auxpass were,determined
R643 T1377 T1373 prep with,associated
R6430 T10085 T10083 prep on,determined
R6431 T10086 T10087 amod anesthetized,mice
R6432 T10087 T10085 pobj mice,on
R6433 T10088 T10083 prep at,determined
R6434 T10089 T10090 det the,time
R6435 T10090 T10088 pobj time,at
R6436 T10091 T10090 prep of,time
R6437 T10092 T10091 pobj sacrifice,of
R6438 T10093 T10083 prep by,determined
R6439 T10094 T10095 amod direct,measurement
R644 T1378 T1379 det an,increase
R6440 T10095 T10093 pobj measurement,by
R6441 T10096 T10093 cc or,by
R6442 T10097 T10093 conj from,by
R6443 T10098 T10099 det the,radiograph
R6444 T10099 T10097 pobj radiograph,from
R6445 T10100 T10099 compound Faxitron,radiograph
R6446 T10101 T10083 punct .,determined
R6447 T10103 T10104 nsubjpass Micro-CT,performed
R6448 T10105 T10104 auxpass was,performed
R6449 T10106 T10104 prep on,performed
R645 T1379 T1377 pobj increase,with
R6450 T10107 T10108 det the,femur
R6451 T10108 T10106 pobj femur,on
R6452 T10109 T10108 amod left,femur
R6453 T10110 T10108 cc and,femur
R6454 T10111 T10112 amod fourth,vertebra
R6455 T10112 T10108 conj vertebra,femur
R6456 T10113 T10112 compound lumbar,vertebra
R6457 T10114 T10104 prep after,performed
R6458 T10115 T10114 pobj removal,after
R6459 T10116 T10115 prep of,removal
R646 T1380 T1379 prep in,increase
R6460 T10117 T10118 amod soft,tissues
R6461 T10118 T10116 pobj tissues,of
R6462 T10119 T10115 cc and,removal
R6463 T10120 T10121 amod overnight,fixation
R6464 T10121 T10115 conj fixation,removal
R6465 T10122 T10121 prep in,fixation
R6466 T10123 T10124 nummod 4,%
R6467 T10124 T10125 compound %,paraformaldehyde
R6468 T10125 T10122 pobj paraformaldehyde,in
R6469 T10126 T10104 punct .,performed
R647 T1381 T1382 compound marrow,fat
R6470 T10128 T10129 det The,metaphysis
R6471 T10129 T10131 nsubjpass metaphysis,scanned
R6472 T10130 T10129 amod distal,metaphysis
R6473 T10132 T10131 auxpass was,scanned
R6474 T10133 T10131 prep with,scanned
R6475 T10134 T10135 det a,instrument
R6476 T10135 T10133 pobj instrument,with
R6477 T10136 T10135 nmod Skyscan,instrument
R6478 T10137 T10136 nummod 1072,Skyscan
R6479 T10138 T10135 compound micro-CT,instrument
R648 T1382 T1380 pobj fat,in
R6480 T10139 T10140 punct (,Skyscan
R6481 T10140 T10135 parataxis Skyscan,instrument
R6482 T10141 T10140 punct ", ",Skyscan
R6483 T10142 T10140 npadvmod Antwerp,Skyscan
R6484 T10143 T10140 punct ", ",Skyscan
R6485 T10144 T10140 npadvmod Belgium,Skyscan
R6486 T10145 T10140 punct ),Skyscan
R6487 T10146 T10131 punct .,scanned
R6488 T10148 T10149 compound Image,acquisition
R6489 T10149 T10150 nsubjpass acquisition,performed
R649 T1383 T1382 punct ", ",fat
R6490 T10151 T10150 auxpass was,performed
R6491 T10152 T10150 prep at,performed
R6492 T10153 T10154 nummod 100,kV
R6493 T10154 T10152 pobj kV,at
R6494 T10155 T10154 cc and,kV
R6495 T10156 T10157 nummod 98,μA
R6496 T10157 T10154 conj μA,kV
R6497 T10158 T10150 punct ", ",performed
R6498 T10159 T10150 prep with,performed
R6499 T10160 T10161 det a,rotation
R650 T1384 T1385 advmod where,favored
R6500 T10161 T10159 pobj rotation,with
R6501 T10162 T10163 nummod 0.9,°
R6502 T10163 T10161 compound °,rotation
R6503 T10164 T10161 prep between,rotation
R6504 T10165 T10164 pobj frames,between
R6505 T10166 T10150 punct .,performed
R6506 T10168 T10169 det The,images
R6507 T10169 T10173 nsubjpass images,used
R6508 T10170 T10171 advmod two,dimensional
R6509 T10171 T10169 amod dimensional,images
R651 T1385 T1382 relcl favored,fat
R6510 T10172 T10171 punct -,dimensional
R6511 T10174 T10173 auxpass were,used
R6512 T10175 T10176 aux to,generate
R6513 T10176 T10173 advcl generate,used
R6514 T10177 T10178 advmod three,dimensional
R6515 T10178 T10180 amod dimensional,reconstructions
R6516 T10179 T10178 punct -,dimensional
R6517 T10180 T10176 dobj reconstructions,generate
R6518 T10181 T10182 aux to,obtain
R6519 T10182 T10176 advcl obtain,generate
R652 T1386 T1387 det the,generation
R6520 T10183 T10184 amod quantitative,data
R6521 T10184 T10182 dobj data,obtain
R6522 T10185 T10182 prep with,obtain
R6523 T10186 T10187 det the,software
R6524 T10187 T10185 pobj software,with
R6525 T10188 T10189 amod 3D,Creator
R6526 T10189 T10187 compound Creator,software
R6527 T10190 T10187 acl supplied,software
R6528 T10191 T10190 prep with,supplied
R6529 T10192 T10193 det the,instrument
R653 T1387 T1385 nsubjpass generation,favored
R6530 T10193 T10191 pobj instrument,with
R6531 T10194 T10173 punct .,used
R6532 T10196 T10197 compound Serum,biochemistry
R6533 T10197 T10198 nsubjpass biochemistry,determined
R6534 T10199 T10200 punct (,Mg
R6535 T10200 T10197 parataxis Mg,biochemistry
R6536 T10201 T10200 nmod ALP,Mg
R6537 T10202 T10200 punct ", ",Mg
R6538 T10203 T10200 nmod Ca,Mg
R6539 T10204 T10200 punct ", ",Mg
R654 T1388 T1387 prep of,generation
R6540 T10205 T10200 nmod PO4,Mg
R6541 T10206 T10200 punct ", ",Mg
R6542 T10207 T10200 punct ),Mg
R6543 T10208 T10198 auxpass was,determined
R6544 T10209 T10198 prep at,determined
R6545 T10210 T10211 det the,Lab
R6546 T10211 T10209 pobj Lab,at
R6547 T10212 T10211 compound Rodent,Lab
R6548 T10213 T10211 compound Diagnostics,Lab
R6549 T10214 T10215 punct (,University
R655 T1389 T1388 pobj adipocytes,of
R6550 T10215 T10211 parataxis University,Lab
R6551 T10216 T10215 compound McGill,University
R6552 T10217 T10215 punct ", ",University
R6553 T10218 T10215 npadvmod Montréal,University
R6554 T10219 T10215 punct ", ",University
R6555 T10220 T10215 npadvmod Quebec,University
R6556 T10221 T10215 punct ", ",University
R6557 T10222 T10215 npadvmod Canada,University
R6558 T10223 T10215 punct ),University
R6559 T10224 T10198 advcl using,determined
R656 T1390 T1385 auxpass is,favored
R6560 T10225 T10226 amod routine,techniques
R6561 T10226 T10224 dobj techniques,using
R6562 T10227 T10226 amod automated,techniques
R6563 T10228 T10198 punct .,determined
R6564 T10230 T10231 amod Commercial,assays
R6565 T10231 T10232 nsubjpass assays,used
R6566 T10233 T10232 auxpass were,used
R6567 T10234 T10235 aux to,determine
R6568 T10235 T10232 advcl determine,used
R6569 T10236 T10237 compound serum,levels
R657 T1391 T1385 prep over,favored
R6570 T10237 T10235 dobj levels,determine
R6571 T10238 T10237 prep of,levels
R6572 T10239 T10238 pobj CTX,of
R6573 T10240 T10241 punct (,ELISA
R6574 T10241 T10239 parataxis ELISA,CTX
R6575 T10242 T10241 compound RatLaps,ELISA
R6576 T10243 T10241 punct ", ",ELISA
R6577 T10244 T10245 compound Nordic,Bioscience
R6578 T10245 T10241 appos Bioscience,ELISA
R6579 T10246 T10241 punct ),ELISA
R658 T1392 T1391 pobj osteoblasts,over
R6580 T10247 T10232 punct ", ",used
R6581 T10248 T10249 nummod 17β,estradiol
R6582 T10249 T10232 dep estradiol,used
R6583 T10250 T10251 punct (,Immuno-Biological
R6584 T10251 T10249 parataxis Immuno-Biological,estradiol
R6585 T10252 T10251 compound IBL,Immuno-Biological
R6586 T10253 T10251 punct ", ",Immuno-Biological
R6587 T10254 T10251 npadvmod Hamburg,Immuno-Biological
R6588 T10255 T10251 punct ", ",Immuno-Biological
R6589 T10256 T10251 npadvmod Germany,Immuno-Biological
R659 T1393 T1394 punct [,23
R6590 T10257 T10251 punct ),Immuno-Biological
R6591 T10258 T10249 punct ", ",estradiol
R6592 T10259 T10249 conj leptin,estradiol
R6593 T10260 T10261 punct (,Systems
R6594 T10261 T10259 parataxis Systems,leptin
R6595 T10262 T10261 nmod R,Systems
R6596 T10263 T10262 cc &,R
R6597 T10264 T10262 conj D,R
R6598 T10265 T10261 punct ", ",Systems
R6599 T10266 T10261 npadvmod Minneapolis,Systems
R660 T1394 T1373 parataxis 23,associated
R6600 T10267 T10261 punct ", ",Systems
R6601 T10268 T10261 npadvmod Minnesota,Systems
R6602 T10269 T10261 punct ", ",Systems
R6603 T10270 T10271 compound United,States
R6604 T10271 T10261 npadvmod States,Systems
R6605 T10272 T10261 punct ),Systems
R6606 T10273 T10259 punct ", ",leptin
R6607 T10274 T10259 cc and,leptin
R6608 T10275 T10259 conj interleukin,leptin
R6609 T10276 T10275 punct -,interleukin
R661 T1395 T1394 punct ],23
R6610 T10277 T10275 nummod 6,interleukin
R6611 T10278 T10279 punct (,Systems
R6612 T10279 T10275 parataxis Systems,interleukin
R6613 T10280 T10279 nmod R,Systems
R6614 T10281 T10280 cc &,R
R6615 T10282 T10280 conj D,R
R6616 T10283 T10279 punct ),Systems
R6617 T10284 T10232 punct .,used
R6618 T10388 T10389 advmod Ex,vivo
R6619 T10389 T10390 amod vivo,assessment
R662 T1396 T1373 punct .,associated
R6620 T10391 T10390 prep of,assessment
R6621 T10392 T10393 nmod osteoblast,activity
R6622 T10393 T10391 pobj activity,of
R6623 T10394 T10392 cc and,osteoblast
R6624 T10395 T10392 conj osteoclast,osteoblast
R6625 T10396 T10390 punct .,assessment
R6626 T10398 T10399 compound Bone,marrow
R6627 T10399 T10400 nsubjpass marrow,flushed
R6628 T10401 T10400 auxpass was,flushed
R6629 T10402 T10400 prep from,flushed
R663 T1398 T1399 nsubjpass Osteoblasts,derived
R6630 T10403 T10404 det the,tibia
R6631 T10404 T10402 pobj tibia,from
R6632 T10405 T10404 cc and,tibia
R6633 T10406 T10404 conj femora,tibia
R6634 T10407 T10404 prep of,tibia
R6635 T10408 T10409 amod juvenile,mice
R6636 T10409 T10407 pobj mice,of
R6637 T10410 T10411 nummod 4,week
R6638 T10411 T10413 npadvmod week,old
R6639 T10412 T10411 punct -,week
R664 T1400 T1398 cc and,Osteoblasts
R6640 T10413 T10409 amod old,mice
R6641 T10414 T10413 punct -,old
R6642 T10415 T10409 nmod Sam68,mice
R6643 T10416 T10415 punct +,Sam68
R6644 T10417 T10415 punct /,Sam68
R6645 T10418 T10415 punct +,Sam68
R6646 T10419 T10415 cc and,Sam68
R6647 T10420 T10415 conj Sam68,Sam68
R6648 T10421 T10420 punct −,Sam68
R6649 T10422 T10420 punct /,Sam68
R665 T1401 T1398 conj adipocytes,Osteoblasts
R6650 T10423 T10420 punct −,Sam68
R6651 T10424 T10425 aux to,obtain
R6652 T10425 T10400 advcl obtain,flushed
R6653 T10426 T10427 amod stromal,cells
R6654 T10427 T10425 dobj cells,obtain
R6655 T10428 T10429 dep that,maintained
R6656 T10429 T10427 relcl maintained,cells
R6657 T10430 T10429 auxpass were,maintained
R6658 T10431 T10429 prep in,maintained
R6659 T10432 T10433 compound differentiation,medium
R666 T1402 T1399 auxpass are,derived
R6660 T10433 T10431 pobj medium,in
R6661 T10434 T10400 prep for,flushed
R6662 T10435 T10436 nummod 6,days
R6663 T10436 T10434 pobj days,for
R6664 T10437 T10435 cc or,6
R6665 T10438 T10435 conj 18,6
R6666 T10439 T10440 mark as,described
R6667 T10440 T10400 advcl described,flushed
R6668 T10441 T10442 punct [,63
R6669 T10442 T10400 parataxis 63,flushed
R667 T1403 T1399 prep from,derived
R6670 T10443 T10442 punct ],63
R6671 T10444 T10400 punct .,flushed
R6672 T10446 T10447 nsubjpass Cultures,stained
R6673 T10448 T10447 auxpass were,stained
R6674 T10449 T10450 advmod in,situ
R6675 T10450 T10447 advmod situ,stained
R6676 T10451 T10447 prep for,stained
R6677 T10452 T10453 compound ALP,activity
R6678 T10453 T10451 pobj activity,for
R6679 T10454 T10451 cc and,for
R668 T1404 T1405 det a,cell
R6680 T10455 T10451 conj with,for
R6681 T10456 T10457 compound von,Kossa
R6682 T10457 T10458 compound Kossa,stain
R6683 T10458 T10455 pobj stain,with
R6684 T10459 T10460 aux to,detect
R6685 T10460 T10447 advcl detect,stained
R6686 T10461 T10462 amod mineralized,nodules
R6687 T10462 T10460 dobj nodules,detect
R6688 T10463 T10447 punct .,stained
R6689 T10465 T10466 amod Total,RNA
R669 T1405 T1403 pobj cell,from
R6690 T10466 T10467 nsubjpass RNA,harvested
R6691 T10468 T10467 auxpass was,harvested
R6692 T10469 T10467 prep from,harvested
R6693 T10470 T10471 amod parallel,cultures
R6694 T10471 T10469 pobj cultures,from
R6695 T10472 T10467 prep for,harvested
R6696 T10473 T10474 compound RT,PCR
R6697 T10474 T10476 compound PCR,analysis
R6698 T10475 T10474 punct -,PCR
R6699 T10476 T10472 pobj analysis,for
R670 T1406 T1405 amod common,cell
R6700 T10477 T10476 prep of,analysis
R6701 T10478 T10479 npadvmod osteoblast,related
R6702 T10479 T10481 amod related,expression
R6703 T10480 T10479 punct -,related
R6704 T10481 T10477 pobj expression,of
R6705 T10482 T10481 compound gene,expression
R6706 T10483 T10467 prep over,harvested
R6707 T10484 T10483 pobj time,over
R6708 T10485 T10486 mark as,described
R6709 T10486 T10467 advcl described,harvested
R671 T1407 T1405 amod mesenchymal,cell
R6710 T10487 T10486 advmod previously,described
R6711 T10488 T10489 punct [,63
R6712 T10489 T10467 parataxis 63,harvested
R6713 T10490 T10489 punct ],63
R6714 T10491 T10467 punct .,harvested
R6715 T10493 T10494 nsubj Osteoclasts,isolated
R6716 T10495 T10494 aux were,isolated
R6717 T10496 T10494 prep from,isolated
R6718 T10497 T10498 det the,bones
R6719 T10498 T10496 pobj bones,from
R672 T1408 T1405 compound precursor,cell
R6720 T10499 T10498 amod crushed,bones
R6721 T10500 T10498 amod long,bones
R6722 T10501 T10498 prep of,bones
R6723 T10502 T10503 amod juvenile,mice
R6724 T10503 T10501 pobj mice,of
R6725 T10504 T10503 nmod Sam68,mice
R6726 T10505 T10504 punct +,Sam68
R6727 T10506 T10504 punct /,Sam68
R6728 T10507 T10504 punct +,Sam68
R6729 T10508 T10504 cc and,Sam68
R673 T1409 T1405 relcl present,cell
R6730 T10509 T10504 conj Sam68,Sam68
R6731 T10510 T10509 punct −,Sam68
R6732 T10511 T10509 punct /,Sam68
R6733 T10512 T10509 punct −,Sam68
R6734 T10513 T10494 cc and,isolated
R6735 T10514 T10494 conj used,isolated
R6736 T10515 T10514 prep for,used
R6737 T10516 T10517 amod quantitative,studies
R6738 T10517 T10515 pobj studies,for
R6739 T10518 T10519 mark as,described
R674 T1410 T1409 prep in,present
R6740 T10519 T10494 advcl described,isolated
R6741 T10520 T10521 punct [,64
R6742 T10521 T10494 parataxis 64,isolated
R6743 T10522 T10521 punct ],64
R6744 T10523 T10494 punct .,isolated
R6745 T10525 T10526 advmod Briefly,plated
R6746 T10527 T10526 punct ", ",plated
R6747 T10528 T10526 nsubjpass cells,plated
R6748 T10529 T10526 auxpass were,plated
R6749 T10530 T10526 prep on,plated
R675 T1411 T1412 compound bone,marrow
R6750 T10531 T10532 compound glass,coverslips
R6751 T10532 T10530 pobj coverslips,on
R6752 T10533 T10530 cc or,on
R6753 T10534 T10530 conj on,on
R6754 T10535 T10536 compound dentin,slices
R6755 T10536 T10534 pobj slices,on
R6756 T10537 T10526 prep in,plated
R6757 T10538 T10539 nummod 24,well
R6758 T10539 T10541 compound well,plates
R6759 T10540 T10539 punct -,well
R676 T1412 T1410 pobj marrow,in
R6760 T10541 T10537 pobj plates,in
R6761 T10542 T10541 compound cluster,plates
R6762 T10543 T10526 prep for,plated
R6763 T10544 T10543 pobj assessment,for
R6764 T10545 T10544 prep of,assessment
R6765 T10546 T10547 compound cell,number
R6766 T10547 T10545 pobj number,of
R6767 T10548 T10547 cc and,number
R6768 T10549 T10550 compound pit,number
R6769 T10550 T10547 conj number,number
R677 T1413 T1399 punct ", ",derived
R6770 T10551 T10526 punct ", ",plated
R6771 T10552 T10526 advmod respectively,plated
R6772 T10553 T10526 punct .,plated
R6773 T10555 T10556 compound Cover,slips
R6774 T10556 T10557 nsubjpass slips,immersed
R6775 T10558 T10557 auxpass were,immersed
R6776 T10559 T10557 prep in,immersed
R6777 T10560 T10561 nummod 4,%
R6778 T10561 T10562 compound %,paraformaldehyde
R6779 T10562 T10559 pobj paraformaldehyde,in
R678 T1414 T1399 cc and,derived
R6780 T10563 T10557 cc and,immersed
R6781 T10564 T10557 conj stained,immersed
R6782 T10565 T10564 prep for,stained
R6783 T10566 T10567 compound TRAP,activity
R6784 T10567 T10565 pobj activity,for
R6785 T10568 T10564 prep after,stained
R6786 T10569 T10570 nummod 2,h
R6787 T10570 T10568 pobj h,after
R6788 T10571 T10557 punct .,immersed
R6789 T10573 T10574 compound Dentin,slices
R679 T1415 T1416 det the,factors
R6790 T10574 T10575 nsubjpass slices,left
R6791 T10576 T10575 auxpass were,left
R6792 T10577 T10575 prep for,left
R6793 T10578 T10579 nummod 28,h
R6794 T10579 T10577 pobj h,for
R6795 T10580 T10575 prep before,left
R6796 T10581 T10580 pcomp removing,before
R6797 T10582 T10583 det the,cells
R6798 T10583 T10581 dobj cells,removing
R6799 T10584 T10581 prep with,removing
R680 T1416 T1417 nsubjpass factors,understood
R6800 T10585 T10586 nummod 1,M
R6801 T10586 T10587 compound M,hydroxide
R6802 T10587 T10584 pobj hydroxide,with
R6803 T10588 T10587 compound ammonium,hydroxide
R6804 T10589 T10581 cc and,removing
R6805 T10590 T10591 dep air,drying
R6806 T10591 T10581 conj drying,removing
R6807 T10592 T10591 prep before,drying
R6808 T10593 T10592 pobj coating,before
R6809 T10594 T10593 prep with,coating
R681 T1417 T1399 conj understood,derived
R6810 T10595 T10596 compound sputter,Pd
R6811 T10596 T10594 pobj Pd,with
R6812 T10597 T10596 compound Au,Pd
R6813 T10598 T10596 punct -,Pd
R6814 T10599 T10593 cc and,coating
R6815 T10600 T10593 conj examination,coating
R6816 T10601 T10600 prep with,examination
R6817 T10602 T10603 amod scanning,microscopy
R6818 T10603 T10601 pobj microscopy,with
R6819 T10604 T10603 compound electron,microscopy
R682 T1418 T1419 dep that,control
R6820 T10605 T10606 punct (,Centre
R6821 T10606 T10603 parataxis Centre,microscopy
R6822 T10607 T10606 compound McGill,Centre
R6823 T10608 T10609 compound Electron,Microscopy
R6824 T10609 T10606 compound Microscopy,Centre
R6825 T10610 T10606 punct ),Centre
R6826 T10611 T10575 punct .,left
R6827 T10613 T10614 compound Light,microscope
R6828 T10614 T10615 compound microscope,images
R6829 T10615 T10616 nsubjpass images,captured
R683 T1419 T1416 relcl control,factors
R6830 T10617 T10616 auxpass were,captured
R6831 T10618 T10616 advcl using,captured
R6832 T10619 T10620 det a,microscope
R6833 T10620 T10618 dobj microscope,using
R6834 T10621 T10622 compound Leica,DMR
R6835 T10622 T10620 compound DMR,microscope
R6836 T10623 T10620 acl equipped,microscope
R6837 T10624 T10623 prep with,equipped
R6838 T10625 T10626 det a,camera
R6839 T10626 T10624 pobj camera,with
R684 T1420 T1421 det this,switch
R6840 T10627 T10626 nmod Retiga,camera
R6841 T10628 T10627 nummod 1300,Retiga
R6842 T10629 T10616 punct .,captured
R6843 T10631 T10632 amod Quantitative,analyses
R6844 T10632 T10633 nsubjpass analyses,performed
R6845 T10634 T10633 auxpass were,performed
R6846 T10635 T10633 advcl using,performed
R6847 T10636 T10637 compound Adobe,Photoshop
R6848 T10637 T10635 dobj Photoshop,using
R6849 T10638 T10637 cc and,Photoshop
R685 T1421 T1419 dobj switch,control
R6850 T10639 T10640 det the,data
R6851 T10640 T10637 conj data,Photoshop
R6852 T10641 T10640 acl presented,data
R6853 T10642 T10641 prep as,presented
R6854 T10643 T10644 det the,SD
R6855 T10644 T10642 pobj SD,as
R6856 T10645 T10644 nmod mean,SD
R6857 T10646 T10644 punct ±,SD
R6858 T10647 T10644 prep of,SD
R6859 T10648 T10649 nummod two,experiments
R686 T1422 T1423 npadvmod age,induced
R6860 T10649 T10647 pobj experiments,of
R6861 T10650 T10648 cc or,two
R6862 T10651 T10648 conj three,two
R6863 T10652 T10649 amod independent,experiments
R6864 T10653 T10633 punct .,performed
R6865 T10655 T10656 amod Statistical,comparisons
R6866 T10656 T10657 nsubjpass comparisons,made
R6867 T10658 T10657 auxpass were,made
R6868 T10659 T10657 advcl using,made
R6869 T10660 T10661 det the,Student
R687 T1423 T1421 amod induced,switch
R6870 T10661 T10662 poss Student,test
R6871 T10662 T10659 dobj test,using
R6872 T10663 T10661 case 's,Student
R6873 T10664 T10662 compound t,test
R6874 T10665 T10657 punct .,made
R6875 T10694 T10693 prep of,Preparation
R6876 T10695 T10696 nmod mouse,fibroblast
R6877 T10696 T10694 pobj fibroblast,of
R6878 T10697 T10696 amod embryonic,fibroblast
R6879 T10698 T10693 cc and,Preparation
R688 T1424 T1423 punct -,induced
R6880 T10699 T10693 conj induction,Preparation
R6881 T10700 T10699 prep of,induction
R6882 T10701 T10702 compound adipocyte,differentiation
R6883 T10702 T10700 pobj differentiation,of
R6884 T10703 T10699 punct .,induction
R6885 T10705 T10706 nsubjpass MEFs,prepared
R6886 T10707 T10706 auxpass were,prepared
R6887 T10708 T10706 prep from,prepared
R6888 T10709 T10710 nummod 14.5,day
R6889 T10710 T10712 npadvmod day,old
R689 T1425 T1421 prep toward,switch
R6890 T10711 T10710 punct -,day
R6891 T10712 T10714 amod old,embryos
R6892 T10713 T10712 punct -,old
R6893 T10714 T10708 pobj embryos,from
R6894 T10715 T10706 punct .,prepared
R6895 T10717 T10718 advmod Only,MEFs
R6896 T10718 T10722 nsubjpass MEFs,used
R6897 T10719 T10720 amod early,passage
R6898 T10720 T10718 compound passage,MEFs
R6899 T10721 T10720 punct -,passage
R690 T1426 T1427 amod adipogenic,differentiation
R6900 T10723 T10722 auxpass were,used
R6901 T10724 T10722 prep for,used
R6902 T10725 T10726 det the,studies
R6903 T10726 T10724 pobj studies,for
R6904 T10727 T10726 amod experimental,studies
R6905 T10728 T10722 punct ", ",used
R6906 T10729 T10722 cc and,used
R6907 T10730 T10731 nsubjpass induction,carried
R6908 T10731 T10722 conj carried,used
R6909 T10732 T10730 prep of,induction
R691 T1427 T1425 pobj differentiation,toward
R6910 T10733 T10734 compound adipocyte,differentiation
R6911 T10734 T10732 pobj differentiation,of
R6912 T10735 T10731 auxpass was,carried
R6913 T10736 T10731 prt out,carried
R6914 T10737 T10731 prep according,carried
R6915 T10738 T10737 prep to,according
R6916 T10739 T10740 det the,methods
R6917 T10740 T10738 pobj methods,to
R6918 T10741 T10742 advmod previously,described
R6919 T10742 T10740 acl described,methods
R692 T1428 T1417 auxpass is,understood
R6920 T10743 T10744 punct [,30
R6921 T10744 T10731 parataxis 30,carried
R6922 T10745 T10744 punct ],30
R6923 T10746 T10731 punct .,carried
R6924 T10748 T10749 amod Cultured,cells
R6925 T10749 T10750 nsubjpass cells,stained
R6926 T10751 T10750 auxpass were,stained
R6927 T10752 T10750 prep with,stained
R6928 T10753 T10754 compound Oil,O
R6929 T10754 T10752 pobj O,with
R693 T1429 T1417 neg not,understood
R6930 T10755 T10754 compound Red,O
R6931 T10756 T10757 mark as,described
R6932 T10757 T10750 advcl described,stained
R6933 T10758 T10759 punct [,29
R6934 T10759 T10750 parataxis 29,stained
R6935 T10760 T10759 punct ],29
R6936 T10761 T10750 punct .,stained
R6937 T10818 T10819 compound RNA,preparation
R6938 T10820 T10819 cc and,preparation
R6939 T10821 T10822 compound RT,PCR
R694 T1430 T1417 advmod well,understood
R6940 T10822 T10819 conj PCR,preparation
R6941 T10823 T10822 punct -,PCR
R6942 T10824 T10825 aux to,assess
R6943 T10825 T10819 advcl assess,preparation
R6944 T10826 T10825 dobj adipogenesis,assess
R6945 T10827 T10825 prep in,assess
R6946 T10828 T10829 nmod Sam68,MEFs
R6947 T10829 T10827 pobj MEFs,in
R6948 T10830 T10828 punct −,Sam68
R6949 T10831 T10828 punct /,Sam68
R695 T1431 T1432 punct [,24
R6950 T10832 T10828 punct −,Sam68
R6951 T10833 T10819 punct .,preparation
R6952 T10835 T10836 amod Total,RNA
R6953 T10836 T10838 nsubjpass RNA,prepared
R6954 T10837 T10836 amod cellular,RNA
R6955 T10839 T10838 auxpass was,prepared
R6956 T10840 T10838 prep by,prepared
R6957 T10841 T10842 compound TRIzol,reagent
R6958 T10842 T10840 pobj reagent,by
R6959 T10843 T10838 prep according,prepared
R696 T1432 T1417 parataxis 24,understood
R6960 T10844 T10843 prep to,according
R6961 T10845 T10846 det the,manufacturer
R6962 T10846 T10847 poss manufacturer,protocol
R6963 T10847 T10844 pobj protocol,to
R6964 T10848 T10846 case 's,manufacturer
R6965 T10849 T10850 punct (,Invitrogen
R6966 T10850 T10847 parataxis Invitrogen,protocol
R6967 T10851 T10850 punct ", ",Invitrogen
R6968 T10852 T10850 npadvmod Carlsbad,Invitrogen
R6969 T10853 T10850 punct ", ",Invitrogen
R697 T1433 T1432 punct ],24
R6970 T10854 T10850 npadvmod California,Invitrogen
R6971 T10855 T10850 punct ", ",Invitrogen
R6972 T10856 T10857 compound United,States
R6973 T10857 T10850 npadvmod States,Invitrogen
R6974 T10858 T10850 punct ),Invitrogen
R6975 T10859 T10838 punct .,prepared
R6976 T10861 T10862 amod Total,RNA
R6977 T10862 T10863 nsubjpass RNA,transcribed
R6978 T10864 T10865 punct (,μg
R6979 T10865 T10862 parataxis μg,RNA
R698 T1434 T1417 punct .,understood
R6980 T10866 T10865 nummod 1,μg
R6981 T10867 T10865 punct ),μg
R6982 T10868 T10863 auxpass was,transcribed
R6983 T10869 T10863 advmod reverse,transcribed
R6984 T10870 T10863 punct -,transcribed
R6985 T10871 T10863 punct ", ",transcribed
R6986 T10872 T10863 cc and,transcribed
R6987 T10873 T10874 compound cDNA,samples
R6988 T10874 T10875 nsubjpass samples,subjected
R6989 T10875 T10863 conj subjected,transcribed
R699 T1436 T1437 mark While,associated
R6990 T10876 T10875 auxpass were,subjected
R6991 T10877 T10875 prep to,subjected
R6992 T10878 T10877 pobj PCR,to
R6993 T10879 T10875 punct .,subjected
R6994 T10881 T10882 compound RT,PCR
R6995 T10882 T10884 nsubjpass PCR,normalized
R6996 T10883 T10882 punct -,PCR
R6997 T10885 T10884 auxpass was,normalized
R6998 T10886 T10884 agent by,normalized
R6999 T10887 T10888 det the,level
R700 T1437 T1444 advcl associated,remains
R7000 T10888 T10886 pobj level,by
R7001 T10889 T10888 amod transcriptional,level
R7002 T10890 T10888 prep of,level
R7003 T10891 T10890 pobj GAPDH,of
R7004 T10892 T10884 punct .,normalized
R7005 T10894 T10895 det The,primers
R7006 T10895 T10902 nsubjpass primers,used
R7007 T10896 T10895 amod following,primers
R7008 T10897 T10895 nummod 5,primers
R7009 T10898 T10897 punct ′,5
R701 T1438 T1439 amod several,proteins
R7010 T10899 T10897 cc and,5
R7011 T10900 T10897 conj 3,5
R7012 T10901 T10900 punct ′,3
R7013 T10903 T10902 auxpass were,used
R7014 T10904 T10905 aux to,evaluate
R7015 T10905 T10902 advcl evaluate,used
R7016 T10906 T10907 amod adipogenic,differentiation
R7017 T10907 T10905 dobj differentiation,evaluate
R7018 T10908 T10902 punct : ,used
R7019 T10909 T10902 dep KLF5,used
R702 T1439 T1437 nsubjpass proteins,associated
R7020 T10910 T10909 punct : ,KLF5
R7021 T10911 T10909 appos 5,KLF5
R7022 T10912 T10911 punct ',5
R7023 T10913 T10911 punct -,5
R7024 T10914 T10915 compound AGA,C
R7025 T10915 T10911 appos C,5
R7026 T10916 T10915 compound CAA,C
R7027 T10917 T10915 compound GCT,C
R7028 T10918 T10915 compound GAG,C
R7029 T10919 T10915 compound ATG,C
R703 T1440 T1439 amod transcriptional,proteins
R7030 T10920 T10915 compound CTG,C
R7031 T10921 T10911 punct -,5
R7032 T10922 T10911 nummod 3,5
R7033 T10923 T10911 punct ′,5
R7034 T10924 T10911 punct ", ",5
R7035 T10925 T10911 appos 5,5
R7036 T10926 T10925 punct ′,5
R7037 T10927 T10925 punct -,5
R7038 T10928 T10929 compound GGC,GC
R7039 T10929 T10925 appos GC,5
R704 T1441 T1439 amod regulatory,proteins
R7040 T10930 T10929 compound AAA,GC
R7041 T10931 T10929 compound CCT,GC
R7042 T10932 T10929 compound CCA,GC
R7043 T10933 T10929 compound GTC,GC
R7044 T10934 T10925 punct -,5
R7045 T10935 T10925 nummod 3,5
R7046 T10936 T10925 punct ′,5
R7047 T10937 T10909 punct ;,KLF5
R7048 T10938 T10909 conj PPARγ,KLF5
R7049 T10939 T10938 punct : ,PPARγ
R705 T1442 T1437 aux have,associated
R7050 T10940 T10938 appos 5,PPARγ
R7051 T10941 T10940 punct ′,5
R7052 T10942 T10940 punct -,5
R7053 T10943 T10944 compound GTG,C
R7054 T10944 T10940 appos C,5
R7055 T10945 T10944 compound CGA,C
R7056 T10946 T10944 compound TCA,C
R7057 T10947 T10944 compound AAG,C
R7058 T10948 T10944 compound TAG,C
R7059 T10949 T10944 compound AAC,C
R706 T1443 T1437 auxpass been,associated
R7060 T10950 T10944 compound CTG,C
R7061 T10951 T10940 punct -,5
R7062 T10952 T10940 nummod 3,5
R7063 T10953 T10940 punct ′,5
R7064 T10954 T10940 punct ", ",5
R7065 T10955 T10940 appos 5,5
R7066 T10956 T10955 punct ′,5
R7067 T10957 T10955 punct -,5
R7068 T10958 T10959 compound CCT,TCG
R7069 T10959 T10955 appos TCG,5
R707 T1445 T1437 prep with,associated
R7070 T10960 T10959 compound ATC,TCG
R7071 T10961 T10959 compound ATA,TCG
R7072 T10962 T10959 compound AAT,TCG
R7073 T10963 T10959 compound AAG,TCG
R7074 T10964 T10959 compound CTT,TCG
R7075 T10965 T10959 compound CAA,TCG
R7076 T10966 T10955 punct -,5
R7077 T10967 T10955 nummod 3,5
R7078 T10968 T10955 punct ′,5
R7079 T10969 T10938 punct ;,PPARγ
R708 T1446 T1447 compound cell,determination
R7080 T10970 T10971 compound β,Actin
R7081 T10971 T10938 conj Actin,PPARγ
R7082 T10972 T10971 punct -,Actin
R7083 T10973 T10971 punct : ,Actin
R7084 T10974 T10971 appos 5,Actin
R7085 T10975 T10974 punct ′,5
R7086 T10976 T10974 punct -,5
R7087 T10977 T10978 compound TAG,GC
R7088 T10978 T10974 appos GC,5
R7089 T10979 T10978 compound GCG,GC
R709 T1447 T1445 pobj determination,with
R7090 T10980 T10978 compound GAC,GC
R7091 T10981 T10978 compound TGT,GC
R7092 T10982 T10978 compound TAC,GC
R7093 T10983 T10978 compound TGA,GC
R7094 T10984 T10974 punct -,5
R7095 T10985 T10974 nummod 3,5
R7096 T10986 T10974 punct ′,5
R7097 T10987 T10974 punct ", ",5
R7098 T10988 T10974 appos 5,5
R7099 T10989 T10988 punct ′,5
R710 T1448 T1447 compound fate,determination
R7100 T10990 T10988 punct -,5
R7101 T10991 T10992 compound AGC,TGG
R7102 T10992 T10988 appos TGG,5
R7103 T10993 T10992 compound CTT,TGG
R7104 T10994 T10992 compound CAT,TGG
R7105 T10995 T10992 compound ACA,TGG
R7106 T10996 T10992 compound TCA,TGG
R7107 T10997 T10992 compound AGT,TGG
R7108 T10998 T10988 punct -,5
R7109 T10999 T10988 nummod 3,5
R711 T1449 T1447 prep of,determination
R7110 T11000 T10988 punct ′,5
R7111 T11001 T10971 punct ;,Actin
R7112 T11002 T10971 cc and,Actin
R7113 T11003 T10971 conj Sam68,Actin
R7114 T11004 T11003 punct : ,Sam68
R7115 T11005 T11003 appos 5,Sam68
R7116 T11006 T11005 punct ′,5
R7117 T11007 T11005 punct -,5
R7118 T11008 T11009 compound GTG,A
R7119 T11009 T11005 appos A,5
R712 T1450 T1451 nmod bone,marrow
R7120 T11010 T11009 compound GAG,A
R7121 T11011 T11009 compound ACC,A
R7122 T11012 T11009 compound CCA,A
R7123 T11013 T11009 compound AAT,A
R7124 T11014 T11009 compound ATG,A
R7125 T11015 T11009 compound CCC,A
R7126 T11016 T11005 punct -,5
R7127 T11017 T11005 nummod 3,5
R7128 T11018 T11005 punct ′,5
R7129 T11019 T11005 cc and,5
R713 T1451 T1452 nmod marrow,cells
R7130 T11020 T11005 conj 5,5
R7131 T11021 T11020 punct ′,5
R7132 T11022 T11020 punct -,5
R7133 T11023 T11024 compound AAA,A
R7134 T11024 T11020 appos A,5
R7135 T11025 T11024 compound CTG,A
R7136 T11026 T11024 compound CTC,A
R7137 T11027 T11024 compound CTG,A
R7138 T11028 T11024 compound ACA,A
R7139 T11029 T11024 compound GAT,A
R714 T1452 T1449 pobj cells,of
R7140 T11030 T11024 compound ATC,A
R7141 T11031 T11020 punct -,5
R7142 T11032 T11020 nummod 3,5
R7143 T11033 T11020 punct ′,5
R7144 T11034 T10902 punct .,used
R7145 T11076 T11077 nmod Sam68,regulation
R7146 T11078 T11077 advmod down,regulation
R7147 T11079 T11077 punct -,regulation
R7148 T11080 T11077 acl using,regulation
R7149 T11081 T11080 dobj Sam68sh,using
R715 T1453 T1452 amod mesenchymal,cells
R7150 T11082 T11077 punct .,regulation
R7151 T11084 T11085 aux To,generate
R7152 T11085 T11086 advcl generate,purchased
R7153 T11087 T11088 det an,shRNA
R7154 T11088 T11085 dobj shRNA,generate
R7155 T11089 T11086 punct ", ",purchased
R7156 T11090 T11091 nummod 64,base
R7157 T11091 T11093 compound base,nucleotides
R7158 T11092 T11091 punct -,base
R7159 T11093 T11086 nsubjpass nucleotides,purchased
R716 T1454 T1452 punct ", ",cells
R7160 T11094 T11093 compound duplex,nucleotides
R7161 T11095 T11093 compound DNA,nucleotides
R7162 T11096 T11086 auxpass were,purchased
R7163 T11097 T11086 prep from,purchased
R7164 T11098 T11097 pobj Invitrogen,from
R7165 T11099 T11086 punct .,purchased
R7166 T11101 T11102 det This,fragment
R7167 T11102 T11103 nsubj fragment,comprised
R7168 T11104 T11105 nummod 19,residues
R7169 T11105 T11103 dobj residues,comprised
R717 T1455 T1452 prep including,cells
R7170 T11106 T11105 compound base,residues
R7171 T11107 T11105 amod specific,residues
R7172 T11108 T11107 prep to,specific
R7173 T11109 T11110 compound mouse,Sam68
R7174 T11110 T11108 pobj Sam68,to
R7175 T11111 T11112 punct (,TTTTTGGAAA
R7176 T11112 T11103 parataxis TTTTTGGAAA,comprised
R7177 T11113 T11112 compound GATCCCC,TTTTTGGAAA
R7178 T11114 T11112 compound AAGATGACGAGGAGAATTATTCAAGAGA,TTTTTGGAAA
R7179 T11115 T11112 compound TAATTCTCCTCGTCATCTT,TTTTTGGAAA
R718 T1456 T1457 nmod peroxisome,receptor
R7180 T11116 T11112 punct ),TTTTTGGAAA
R7181 T11117 T11103 punct .,comprised
R7182 T11119 T11120 det This,fragment
R7183 T11120 T11121 nsubjpass fragment,cloned
R7184 T11122 T11121 auxpass was,cloned
R7185 T11123 T11121 prep into,cloned
R7186 T11124 T11125 compound pSUPER,retro
R7187 T11125 T11123 pobj retro,into
R7188 T11126 T11127 punct (,OligoEngine
R7189 T11127 T11125 parataxis OligoEngine,retro
R719 T1457 T1455 pobj receptor,including
R7190 T11128 T11127 punct ", ",OligoEngine
R7191 T11129 T11127 npadvmod Seattle,OligoEngine
R7192 T11130 T11127 punct ", ",OligoEngine
R7193 T11131 T11127 npadvmod Washington,OligoEngine
R7194 T11132 T11127 punct ", ",OligoEngine
R7195 T11133 T11134 compound United,States
R7196 T11134 T11127 npadvmod States,OligoEngine
R7197 T11135 T11127 punct ),OligoEngine
R7198 T11136 T11121 punct .,cloned
R7199 T11138 T11139 nsubjpass Transfections,performed
R720 T1458 T1459 npadvmod proliferator,activated
R7200 T11140 T11139 auxpass were,performed
R7201 T11141 T11139 advcl using,performed
R7202 T11142 T11143 compound LipofectAMINE,Plus
R7203 T11143 T11141 dobj Plus,using
R7204 T11144 T11145 punct (,Invitrogen
R7205 T11145 T11143 parataxis Invitrogen,Plus
R7206 T11146 T11145 punct ),Invitrogen
R7207 T11147 T11141 prep with,using
R7208 T11148 T11149 amod cloned,fragment
R7209 T11149 T11147 pobj fragment,with
R721 T1459 T1457 amod activated,receptor
R7210 T11150 T11149 compound DNA,fragment
R7211 T11151 T11149 cc or,fragment
R7212 T11152 T11153 amod empty,vector
R7213 T11153 T11149 conj vector,fragment
R7214 T11154 T11139 punct .,performed
R7215 T11156 T11157 prep At,selected
R7216 T11158 T11159 nummod 48,h
R7217 T11159 T11156 pobj h,At
R7218 T11160 T11159 prep after,h
R7219 T11161 T11160 pobj transfection,after
R722 T1460 T1459 punct –,activated
R7220 T11162 T11157 punct ", ",selected
R7221 T11163 T11157 nsubjpass populations,selected
R7222 T11164 T11157 auxpass were,selected
R7223 T11165 T11157 prep for,selected
R7224 T11166 T11167 nummod 2.5,μg
R7225 T11167 T11168 nmod μg,puromycin
R7226 T11168 T11165 pobj puromycin,for
R7227 T11169 T11170 punct /,ml
R7228 T11170 T11167 prep ml,μg
R7229 T11171 T11157 cc and,selected
R723 T1461 T1457 punct γ,receptor
R7230 T11172 T11173 det the,knockdown
R7231 T11173 T11174 nsubj knockdown,observed
R7232 T11174 T11157 conj observed,selected
R7233 T11175 T11174 prep by,observed
R7234 T11176 T11175 pcomp immunoblotting,by
R7235 T11177 T11176 prep with,immunoblotting
R7236 T11178 T11179 nmod anti-Sam68,antibody
R7237 T11179 T11177 pobj antibody,with
R7238 T11180 T11181 punct (,AD1
R7239 T11181 T11178 parataxis AD1,anti-Sam68
R724 T1462 T1457 punct (,receptor
R7240 T11182 T11181 punct ),AD1
R7241 T11183 T11157 punct .,selected
R7242 T11232 T11233 amod Osteogenic,analysis
R7243 T11234 T11233 compound differentiation,analysis
R7244 T11235 T11233 prep of,analysis
R7245 T11236 T11237 nmod C3HT101,cells
R7246 T11237 T11235 pobj cells,of
R7247 T11238 T11236 punct /,C3HT101
R7248 T11239 T11236 nummod 2,C3HT101
R7249 T11240 T11233 punct .,analysis
R725 T1463 T1457 appos PPARγ,receptor
R7250 T11242 T11243 prep For,incubated
R7251 T11244 T11245 amod osteogenic,differentiation
R7252 T11245 T11242 pobj differentiation,For
R7253 T11246 T11243 punct ", ",incubated
R7254 T11247 T11248 nmod C3HT101,cells
R7255 T11248 T11243 nsubjpass cells,incubated
R7256 T11249 T11247 punct /,C3HT101
R7257 T11250 T11247 nummod 2,C3HT101
R7258 T11251 T11252 punct (,ATCC
R7259 T11252 T11247 parataxis ATCC,C3HT101
R726 T1464 T1457 punct ),receptor
R7260 T11253 T11252 punct ),ATCC
R7261 T11254 T11243 auxpass were,incubated
R7262 T11255 T11243 prep in,incubated
R7263 T11256 T11257 nummod 48,well
R7264 T11257 T11259 compound well,plates
R7265 T11258 T11257 punct -,well
R7266 T11259 T11255 pobj plates,in
R7267 T11260 T11259 compound tissue,plates
R7268 T11261 T11259 compound culture,plates
R7269 T11262 T11243 prep at,incubated
R727 T1465 T1457 cc and,receptor
R7270 T11263 T11264 quantmod 1.25,104
R7271 T11264 T11266 nummod 104,cells
R7272 T11265 T11264 punct ×,104
R7273 T11266 T11262 pobj cells,at
R7274 T11267 T11268 punct /,cm2
R7275 T11268 T11266 prep cm2,cells
R7276 T11269 T11243 punct .,incubated
R7277 T11271 T11272 prep After,changed
R7278 T11273 T11274 nummod 24,h
R7279 T11274 T11276 compound h,incubation
R728 T1466 T1457 conj KLF5,receptor
R7280 T11275 T11274 punct -,h
R7281 T11276 T11271 pobj incubation,After
R7282 T11277 T11272 punct ", ",changed
R7283 T11278 T11279 det the,media
R7284 T11279 T11272 nsubjpass media,changed
R7285 T11280 T11279 compound culture,media
R7286 T11281 T11272 auxpass were,changed
R7287 T11282 T11272 prep to,changed
R7288 T11283 T11284 amod fresh,media
R7289 T11284 T11282 pobj media,to
R729 T1467 T1468 punct [,25
R7290 T11285 T11284 acl containing,media
R7291 T11286 T11287 nummod 300,ng
R7292 T11287 T11288 nmod ng,BMP
R7293 T11288 T11285 dobj BMP,containing
R7294 T11289 T11290 punct /,ml
R7295 T11290 T11287 prep ml,ng
R7296 T11291 T11288 punct -,BMP
R7297 T11292 T11288 nummod 2,BMP
R7298 T11293 T11294 punct (,Sigma
R7299 T11294 T11288 parataxis Sigma,BMP
R730 T1468 T1437 parataxis 25,associated
R7300 T11295 T11294 punct ", ",Sigma
R7301 T11296 T11297 compound St.,Louis
R7302 T11297 T11294 npadvmod Louis,Sigma
R7303 T11298 T11294 punct ", ",Sigma
R7304 T11299 T11294 npadvmod Missouri,Sigma
R7305 T11300 T11294 punct ", ",Sigma
R7306 T11301 T11302 compound United,States
R7307 T11302 T11294 npadvmod States,Sigma
R7308 T11303 T11294 punct ),Sigma
R7309 T11304 T11272 punct .,changed
R731 T1469 T1470 punct –,30
R7310 T11306 T11307 amod Total,RNA
R7311 T11307 T11309 nsubjpass RNA,prepared
R7312 T11308 T11307 amod cellular,RNA
R7313 T11310 T11309 auxpass was,prepared
R7314 T11311 T11312 mark as,described
R7315 T11312 T11309 advcl described,prepared
R7316 T11313 T11312 advmod above,described
R7317 T11314 T11309 punct ", ",prepared
R7318 T11315 T11309 cc and,prepared
R7319 T11316 T11317 nmod osteocalcin,expression
R732 T1470 T1468 prep 30,25
R7320 T11317 T11326 nsubjpass expression,quantified
R7321 T11318 T11316 punct ", ",osteocalcin
R7322 T11319 T11320 compound β,actin
R7323 T11320 T11316 conj actin,osteocalcin
R7324 T11321 T11320 punct -,actin
R7325 T11322 T11320 punct ", ",actin
R7326 T11323 T11320 cc and,actin
R7327 T11324 T11320 conj GAPDH,actin
R7328 T11325 T11317 compound gene,expression
R7329 T11326 T11309 conj quantified,prepared
R733 T1471 T1468 punct ],25
R7330 T11327 T11317 prep at,expression
R7331 T11328 T11329 amod indicated,times
R7332 T11329 T11327 pobj times,at
R7333 T11330 T11329 prep after,times
R7334 T11331 T11332 nmod BMP,treatment
R7335 T11332 T11330 pobj treatment,after
R7336 T11333 T11331 punct -,BMP
R7337 T11334 T11331 nummod 2,BMP
R7338 T11335 T11326 auxpass was,quantified
R7339 T11336 T11326 prep by,quantified
R734 T1472 T1444 punct ", ",remains
R7340 T11337 T11338 compound RT,PCR
R7341 T11338 T11336 pobj PCR,by
R7342 T11339 T11338 punct -,PCR
R7343 T11340 T11326 punct .,quantified
R735 T1473 T1474 det the,role
R736 T1474 T1444 nsubj role,remains
R737 T1475 T1474 prep of,role
R738 T1476 T1477 compound RNA,proteins
R739 T1477 T1475 pobj proteins,of
R740 T1478 T1477 compound binding,proteins
R741 T1479 T1474 prep in,role
R742 T1480 T1481 det this,process
R743 T1481 T1479 pobj process,in
R744 T1482 T1444 acomp unknown,remains
R745 T1483 T1444 punct .,remains
R746 T1485 T1486 compound RNA,proteins
R747 T1486 T1488 nsubj proteins,are
R748 T1487 T1486 compound binding,proteins
R749 T1489 T1486 prep of,proteins
R750 T1490 T1491 det the,type
R751 T1491 T1489 pobj type,of
R752 T1492 T1491 compound KH,type
R753 T1493 T1494 amod known,regulators
R754 T1494 T1488 attr regulators,are
R755 T1495 T1494 prep of,regulators
R756 T1496 T1497 amod cellular,differentiation
R757 T1497 T1495 pobj differentiation,of
R758 T1498 T1488 punct .,are
R759 T1500 T1501 prep For,known
R760 T1502 T1500 pobj example,For
R761 T1503 T1501 punct ", ",known
R762 T1504 T1505 compound expression,defects
R763 T1505 T1501 nsubjpass defects,known
R764 T1506 T1505 prep in,defects
R765 T1507 T1508 det the,proteins
R766 T1508 T1506 pobj proteins,in
R767 T1509 T1510 compound KH,domain
R768 T1510 T1508 compound domain,proteins
R769 T1511 T1508 appos NOVA,proteins
R770 T1512 T1511 cc and,NOVA
R771 T1513 T1511 conj FMRP,NOVA
R772 T1514 T1501 auxpass are,known
R773 T1515 T1516 aux to,cause
R774 T1516 T1501 xcomp cause,known
R775 T1517 T1518 amod paraneoplastic,disorders
R776 T1518 T1516 dobj disorders,cause
R777 T1519 T1518 amod neurologic,disorders
R778 T1520 T1521 punct [,31
R779 T1521 T1518 parataxis 31,disorders
R780 T1522 T1521 punct ],31
R781 T1523 T1518 cc and,disorders
R782 T1524 T1525 det the,syndrome
R783 T1525 T1518 conj syndrome,disorders
R784 T1526 T1527 amod fragile,X
R785 T1527 T1525 compound X,syndrome
R786 T1528 T1516 punct ", ",cause
R787 T1529 T1516 advmod respectively,cause
R788 T1530 T1516 punct ", ",cause
R789 T1531 T1516 prep in,cause
R790 T1532 T1531 pobj humans,in
R791 T1533 T1534 punct [,32
R792 T1534 T1516 parataxis 32,cause
R793 T1535 T1534 punct ],32
R794 T1536 T1501 punct .,known
R795 T1538 T1539 det The,phenotype
R796 T1539 T1540 nsubj phenotype,suggests
R797 T1541 T1539 prep of,phenotype
R798 T1542 T1543 det the,mice
R799 T1543 T1541 pobj mice,of
R800 T1544 T1543 nmod quaking,mice
R801 T1545 T1543 amod viable,mice
R802 T1546 T1547 det a,role
R803 T1547 T1540 dobj role,suggests
R804 T1548 T1547 prep for,role
R805 T1549 T1550 det the,protein
R806 T1550 T1548 pobj protein,for
R807 T1551 T1550 compound QUAKING,protein
R808 T1552 T1553 compound RNA,binding
R809 T1553 T1550 compound binding,protein
R810 T1554 T1547 prep in,role
R811 T1555 T1554 pobj oligodendrocytes,in
R812 T1556 T1555 cc and,oligodendrocytes
R813 T1557 T1555 conj myelination,oligodendrocytes
R814 T1558 T1559 punct [,33
R815 T1559 T1540 parataxis 33,suggests
R816 T1560 T1559 punct ],33
R817 T1561 T1540 punct .,suggests
R818 T1563 T1564 advmod Indeed,led
R819 T1565 T1564 punct ", ",led
R820 T1566 T1567 det the,expression
R821 T1567 T1564 nsubj expression,led
R822 T1568 T1567 amod ectopic,expression
R823 T1569 T1567 prep of,expression
R824 T1570 T1571 det the,isoforms
R825 T1571 T1569 pobj isoforms,of
R826 T1572 T1573 nmod QKI,7
R827 T1573 T1571 nummod 7,isoforms
R828 T1574 T1573 punct -,7
R829 T1575 T1573 nummod 6,7
R830 T1576 T1573 punct /,7
R831 T1577 T1578 advmod in,vivo
R832 T1578 T1564 advmod vivo,led
R833 T1579 T1564 prep to,led
R834 T1580 T1581 det the,formation
R835 T1581 T1579 pobj formation,to
R836 T1582 T1581 prep of,formation
R837 T1583 T1584 amod glial,cells
R838 T1584 T1582 pobj cells,of
R839 T1585 T1586 advmod rather,than
R840 T1586 T1584 cc than,cells
R841 T1587 T1584 conj neurons,cells
R842 T1588 T1581 prep from,formation
R843 T1589 T1590 amod neural,progenitor
R844 T1590 T1588 pobj progenitor,from
R845 T1591 T1564 punct ", ",led
R846 T1592 T1564 advcl demonstrating,led
R847 T1593 T1594 poss its,role
R848 T1594 T1592 dobj role,demonstrating
R849 T1595 T1594 prep in,role
R850 T1596 T1597 compound cell,determination
R851 T1597 T1595 pobj determination,in
R852 T1598 T1597 compound fate,determination
R853 T1599 T1600 punct [,34
R854 T1600 T1564 parataxis 34,led
R855 T1601 T1600 punct ],34
R856 T1602 T1564 punct .,led
R857 T1604 T1605 det The,loss
R858 T1605 T1606 nsubj loss,prevents
R859 T1607 T1605 prep of,loss
R860 T1608 T1609 nmod GLD,protein
R861 T1609 T1607 pobj protein,of
R862 T1610 T1608 punct -,GLD
R863 T1611 T1608 nummod 1,GLD
R864 T1612 T1605 prep in,loss
R865 T1613 T1614 compound Caenorhabditis,elegans
R866 T1614 T1612 pobj elegans,in
R867 T1615 T1616 det the,appearance
R868 T1616 T1606 dobj appearance,prevents
R869 T1617 T1616 prep of,appearance
R870 T1618 T1619 compound stem,cells
R871 T1619 T1617 pobj cells,of
R872 T1620 T1621 punct [,35
R873 T1621 T1606 parataxis 35,prevents
R874 T1622 T1621 punct ],35
R875 T1623 T1606 punct ", ",prevents
R876 T1624 T1606 cc and,prevents
R877 T1625 T1626 det the,absence
R878 T1626 T1627 nsubj absence,prevents
R879 T1627 T1606 conj prevents,prevents
R880 T1628 T1626 prep of,absence
R881 T1629 T1630 det the,protein
R882 T1630 T1628 pobj protein,of
R883 T1631 T1630 compound KH,protein
R884 T1632 T1630 compound domain,protein
R885 T1633 T1630 appos HOW,protein
R886 T1634 T1626 prep in,absence
R887 T1635 T1634 pobj Drosophila,in
R888 T1636 T1637 compound muscle,differentiation
R889 T1637 T1627 dobj differentiation,prevents
R890 T1638 T1627 cc and,prevents
R891 T1639 T1627 conj results,prevents
R892 T1640 T1639 prep in,results
R893 T1641 T1640 pobj flies,in
R894 T1642 T1641 prep with,flies
R895 T1643 T1644 amod held,wings
R896 T1644 T1642 pobj wings,with
R897 T1645 T1643 punct -,held
R898 T1646 T1643 prt out,held
R899 T1647 T1644 punct -,wings
R900 T1648 T1649 punct [,37
R901 T1649 T1639 parataxis 37,results
R902 T1650 T1649 nummod 36,37
R903 T1651 T1649 punct ",",37
R904 T1652 T1649 punct ],37
R905 T1653 T1627 punct .,prevents
R906 T1655 T1656 det The,substrate
R907 T1656 T1658 nsubj substrate,is
R908 T1657 T1656 compound Src,substrate
R909 T1658 T1668 ccomp is,remained
R910 T1659 T1656 acl associated,substrate
R911 T1660 T1659 prep in,associated
R912 T1661 T1660 pobj mitosis,in
R913 T1662 T1661 prep of,mitosis
R914 T1663 T1664 nummod 68,kDa
R915 T1664 T1662 pobj kDa,of
R916 T1665 T1666 punct (,Sam68
R917 T1666 T1659 parataxis Sam68,associated
R918 T1667 T1666 punct ),Sam68
R919 T1669 T1658 advmod also,is
R920 T1670 T1671 det a,member
R921 T1671 T1658 attr member,is
R922 T1672 T1671 prep of,member
R923 T1673 T1674 det the,family
R924 T1674 T1672 pobj family,of
R925 T1675 T1674 prep of,family
R926 T1676 T1677 compound KH,proteins
R927 T1677 T1675 pobj proteins,of
R928 T1678 T1677 compound domain,proteins
R929 T1679 T1680 compound RNA,binding
R930 T1680 T1677 compound binding,proteins
R931 T1681 T1682 punct [,38
R932 T1682 T1658 parataxis 38,is
R933 T1683 T1682 punct ],38
R934 T1684 T1668 punct ;,remained
R935 T1685 T1668 advmod however,remained
R936 T1686 T1668 punct ", ",remained
R937 T1687 T1688 poss its,role
R938 T1688 T1668 nsubj role,remained
R939 T1689 T1688 amod physiologic,role
R940 T1690 T1668 aux has,remained
R941 T1691 T1668 acomp undefined,remained
R942 T1692 T1668 punct .,remained
R943 T1721 T1719 prep 42,39
R944 T1694 T1695 nsubjpass Sam68,identified
R945 T1722 T1719 punct ],39
R946 T1723 T1715 cc and,of
R947 T1724 T1715 conj of,of
R948 T1725 T1726 det the,kinase
R949 T1696 T1695 auxpass was,identified
R950 T1726 T1724 pobj kinase,of
R951 T1727 T1728 compound breast,tumor
R952 T1728 T1726 compound tumor,kinase
R953 T1729 T1730 punct [,43
R954 T1697 T1695 prep as,identified
R955 T1730 T1726 parataxis 43,kinase
R956 T1731 T1730 punct ],43
R957 T1698 T1699 det an,protein
R958 T1732 T1695 punct .,identified
R959 T1734 T1735 nsubjpass Sam68,shown
R960 T1736 T1735 aux has,shown
R961 T1737 T1735 auxpass been,shown
R962 T1699 T1697 pobj protein,as
R963 T1738 T1739 aux to,facilitate
R964 T1700 T1699 nmod SH3,protein
R965 T1739 T1735 xcomp facilitate,shown
R966 T1701 T1700 cc and,SH3
R967 T1702 T1700 conj SH2,SH3
R968 T1740 T1741 det the,export
R969 T1703 T1699 nmod domain,protein
R970 T1741 T1739 dobj export,facilitate
R971 T1704 T1699 amod interacting,protein
R972 T1742 T1741 prep of,export
R973 T1743 T1744 amod unspliced,RNA
R974 T1744 T1742 pobj RNA,of
R975 T1705 T1695 prep for,identified
R976 T1745 T1744 compound HIV,RNA
R977 T1746 T1747 punct [,44
R978 T1747 T1739 parataxis 44,facilitate
R979 T1706 T1707 compound Src,kinases
R980 T1748 T1747 punct ],44
R981 T1749 T1739 cc and,facilitate
R982 T1750 T1751 aux to,regulate
R983 T1707 T1705 pobj kinases,for
R984 T1751 T1739 conj regulate,facilitate
R985 T1752 T1753 compound pre-mRNA,processing
R986 T1708 T1707 compound family,kinases
R987 T1753 T1751 dobj processing,regulate
R988 T1754 T1755 punct [,45
R989 T1755 T1751 parataxis 45,regulate
R990 T1709 T1695 cc and,identified
R991 T1756 T1755 punct ],45
R992 T1757 T1735 punct .,shown
R993 T1759 T1760 prep In,report
R994 T1710 T1695 conj is,identified
R995 T1761 T1762 det the,paper
R996 T1711 T1710 advmod also,is
R997 T1762 T1759 pobj paper,In
R998 T1763 T1762 amod present,paper
R999 T1764 T1760 punct ", ",report