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PMC:1315279 / 27288-28296 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T6007 0-2 TO denotes To
T6009 3-10 RB denotes further
T6008 11-18 VB denotes examine
T6011 19-23 DT denotes this
T6012 24-33 NN denotes phenotype
T6013 34-36 IN denotes in
T6014 37-38 DT denotes a
T6016 39-43 NN denotes cell
T6017 44-54 JJ denotes autonomous
T6015 55-61 NN denotes system
T6018 61-63 , denotes ,
T6019 63-65 PRP denotes we
T6010 66-71 VBD denotes chose
T6020 72-75 DT denotes the
T6022 76-85 JJ denotes embryonic
T6023 86-97 JJ denotes mesenchymal
T6024 98-112 JJ denotes multipotential
T6025 113-123 NN denotes progenitor
T6021 124-129 NNS denotes cells
T6026 130-137 NN denotes C3H10T1
T6027 137-138 HYPH denotes /
T6028 138-139 CD denotes 2
T6029 139-140 . denotes .
T6030 140-299 sentence denotes The addition of BMP-2 induced osteoblast differentiation, as evidenced by an approximately 400-fold increase in expression of the osteocalcin (OCN) gene [51].
T6031 141-144 DT denotes The
T6032 145-153 NN denotes addition
T6034 154-156 IN denotes of
T6035 157-160 NN denotes BMP
T6036 160-161 HYPH denotes -
T6037 161-162 CD denotes 2
T6033 163-170 VBD denotes induced
T6038 171-181 NN denotes osteoblast
T6039 182-197 NN denotes differentiation
T6040 197-199 , denotes ,
T6041 199-201 IN denotes as
T6042 202-211 VBN denotes evidenced
T6043 212-214 IN denotes by
T6044 215-217 DT denotes an
T6046 218-231 RB denotes approximately
T6047 232-240 JJ denotes 400-fold
T6045 241-249 NN denotes increase
T6048 250-252 IN denotes in
T6049 253-263 NN denotes expression
T6050 264-266 IN denotes of
T6051 267-270 DT denotes the
T6053 271-282 NN denotes osteocalcin
T6054 283-284 -LRB- denotes (
T6055 284-287 NN denotes OCN
T6056 287-288 -RRB- denotes )
T6052 289-293 NN denotes gene
T6057 294-295 -LRB- denotes [
T6058 295-297 CD denotes 51
T6059 297-298 -RRB- denotes ]
T6060 298-299 . denotes .
T6061 299-481 sentence denotes Populations of C3H10T1/2 cells stably transfected with either pSuper-retro (control) and pSuper-retro harboring a short hairpin against Sam68 (Sam68sh) were selected with puromycin.
T6062 300-311 NNS denotes Populations
T6064 312-314 IN denotes of
T6065 315-322 NN denotes C3H10T1
T6067 322-323 HYPH denotes /
T6068 323-324 CD denotes 2
T6066 325-330 NNS denotes cells
T6069 331-337 RB denotes stably
T6070 338-349 VBN denotes transfected
T6071 350-354 IN denotes with
T6072 355-361 CC denotes either
T6074 362-368 NN denotes pSuper
T6075 368-369 HYPH denotes -
T6073 369-374 NN denotes retro
T6076 375-376 -LRB- denotes (
T6077 376-383 NN denotes control
T6078 383-384 -RRB- denotes )
T6079 385-388 CC denotes and
T6080 389-395 NN denotes pSuper
T6082 395-396 HYPH denotes -
T6081 396-401 NN denotes retro
T6083 402-411 VBG denotes harboring
T6084 412-413 DT denotes a
T6086 414-419 JJ denotes short
T6085 420-427 NN denotes hairpin
T6087 428-435 IN denotes against
T6088 436-441 NN denotes Sam68
T6089 442-443 -LRB- denotes (
T6090 443-450 NN denotes Sam68sh
T6091 450-451 -RRB- denotes )
T6092 452-456 VBD denotes were
T6063 457-465 VBN denotes selected
T6093 466-470 IN denotes with
T6094 471-480 NN denotes puromycin
T6095 480-481 . denotes .
T6096 481-622 sentence denotes The expression of Sam68 was reduced by approximately 80% as evidenced by immunoblot analyses using β-actin as a loading control (Figure 8A).
T6097 482-485 DT denotes The
T6098 486-496 NN denotes expression
T6100 497-499 IN denotes of
T6101 500-505 NN denotes Sam68
T6102 506-509 VBD denotes was
T6099 510-517 VBN denotes reduced
T6103 518-520 IN denotes by
T6104 521-534 RB denotes approximately
T6105 535-537 CD denotes 80
T6106 537-538 NN denotes %
T6107 539-541 IN denotes as
T6108 542-551 VBN denotes evidenced
T6109 552-554 IN denotes by
T6110 555-565 NN denotes immunoblot
T6111 566-574 NNS denotes analyses
T6112 575-580 VBG denotes using
T6113 581-582 NN denotes β
T6115 582-583 HYPH denotes -
T6114 583-588 NN denotes actin
T6116 589-591 IN denotes as
T6117 592-593 DT denotes a
T6119 594-601 NN denotes loading
T6118 602-609 NN denotes control
T6120 610-611 -LRB- denotes (
T6122 611-617 NN denotes Figure
T6121 618-620 NN denotes 8A
T6123 620-621 -RRB- denotes )
T6124 621-622 . denotes .
T6125 622-767 sentence denotes Osteoblast differentiation was induced in cultures expressing pSuper-retro or pSuper-retro Sam68sh by addition of BMP-2 to the culture medium. .
T6126 623-633 NN denotes Osteoblast
T6127 634-649 NN denotes differentiation
T6129 650-653 VBD denotes was
T6128 654-661 VBN denotes induced
T6130 662-664 IN denotes in
T6131 665-673 NNS denotes cultures
T6132 674-684 VBG denotes expressing
T6133 685-691 NN denotes pSuper
T6135 691-692 HYPH denotes -
T6134 692-697 NN denotes retro
T6136 698-700 CC denotes or
T6137 701-707 NN denotes pSuper
T6139 707-708 HYPH denotes -
T6138 708-713 NN denotes retro
T6140 714-721 NN denotes Sam68sh
T6141 722-724 IN denotes by
T6142 725-733 NN denotes addition
T6143 734-736 IN denotes of
T6144 737-740 NN denotes BMP
T6145 740-741 HYPH denotes -
T6146 741-742 CD denotes 2
T6147 743-745 IN denotes to
T6148 746-749 DT denotes the
T6150 750-757 NN denotes culture
T6149 758-764 NN denotes medium
T6151 764-765 . denotes .
T6152 766-767 . denotes .
T6153 767-1008 sentence denotes The expression of OCN and β-actin mRNAs was examined by semiquantitative RT-PCR and the Sam68sh-expressing cells displayed a more pronounced osteoblast phenotype compared with control cells, as assessed by the expression of OCN (Figure 8B).
T6154 768-771 DT denotes The
T6155 772-782 NN denotes expression
T6157 783-785 IN denotes of
T6158 786-789 NN denotes OCN
T6159 790-793 CC denotes and
T6160 794-795 NN denotes β
T6162 795-796 HYPH denotes -
T6161 796-801 NN denotes actin
T6163 802-807 NNS denotes mRNAs
T6164 808-811 VBD denotes was
T6156 812-820 VBN denotes examined
T6165 821-823 IN denotes by
T6166 824-840 JJ denotes semiquantitative
T6168 841-843 NN denotes RT
T6169 843-844 HYPH denotes -
T6167 844-847 NN denotes PCR
T6170 848-851 CC denotes and
T6171 852-855 DT denotes the
T6173 856-863 NN denotes Sam68sh
T6175 863-864 HYPH denotes -
T6174 864-874 VBG denotes expressing
T6172 875-880 NNS denotes cells
T6176 881-890 VBD denotes displayed
T6177 891-892 DT denotes a
T6179 893-897 RBR denotes more
T6180 898-908 JJ denotes pronounced
T6181 909-919 NN denotes osteoblast
T6178 920-929 NN denotes phenotype
T6182 930-938 VBN denotes compared
T6183 939-943 IN denotes with
T6184 944-951 NN denotes control
T6185 952-957 NNS denotes cells
T6186 957-959 , denotes ,
T6187 959-961 IN denotes as
T6188 962-970 VBN denotes assessed
T6189 971-973 IN denotes by
T6190 974-977 DT denotes the
T6191 978-988 NN denotes expression
T6192 989-991 IN denotes of
T6193 992-995 NN denotes OCN
T6194 996-997 -LRB- denotes (
T6196 997-1003 NN denotes Figure
T6195 1004-1006 NN denotes 8B
T6197 1006-1007 -RRB- denotes )
T6198 1007-1008 . denotes .
R3675 T6007 T6008 aux To,examine
R3676 T6008 T6010 advcl examine,chose
R3677 T6009 T6008 advmod further,examine
R3678 T6011 T6012 det this,phenotype
R3679 T6012 T6008 dobj phenotype,examine
R3680 T6013 T6008 prep in,examine
R3681 T6014 T6015 det a,system
R3682 T6015 T6013 pobj system,in
R3683 T6016 T6017 npadvmod cell,autonomous
R3684 T6017 T6015 amod autonomous,system
R3685 T6018 T6010 punct ", ",chose
R3686 T6019 T6010 nsubj we,chose
R3687 T6020 T6021 det the,cells
R3688 T6021 T6010 dobj cells,chose
R3689 T6022 T6021 amod embryonic,cells
R3690 T6023 T6021 amod mesenchymal,cells
R3691 T6024 T6021 amod multipotential,cells
R3692 T6025 T6021 compound progenitor,cells
R3693 T6026 T6021 appos C3H10T1,cells
R3694 T6027 T6026 punct /,C3H10T1
R3695 T6028 T6026 nummod 2,C3H10T1
R3696 T6029 T6010 punct .,chose
R3697 T6031 T6032 det The,addition
R3698 T6032 T6033 nsubj addition,induced
R3699 T6034 T6032 prep of,addition
R3700 T6035 T6034 pobj BMP,of
R3701 T6036 T6035 punct -,BMP
R3702 T6037 T6035 nummod 2,BMP
R3703 T6038 T6039 compound osteoblast,differentiation
R3704 T6039 T6033 dobj differentiation,induced
R3705 T6040 T6033 punct ", ",induced
R3706 T6041 T6042 mark as,evidenced
R3707 T6042 T6033 advcl evidenced,induced
R3708 T6043 T6042 agent by,evidenced
R3709 T6044 T6045 det an,increase
R3710 T6045 T6043 pobj increase,by
R3711 T6046 T6047 advmod approximately,400-fold
R3712 T6047 T6045 nmod 400-fold,increase
R3713 T6048 T6045 prep in,increase
R3714 T6049 T6048 pobj expression,in
R3715 T6050 T6049 prep of,expression
R3716 T6051 T6052 det the,gene
R3717 T6052 T6050 pobj gene,of
R3718 T6053 T6052 nmod osteocalcin,gene
R3719 T6054 T6053 punct (,osteocalcin
R3720 T6055 T6053 appos OCN,osteocalcin
R3721 T6056 T6052 punct ),gene
R3722 T6057 T6058 punct [,51
R3723 T6058 T6033 parataxis 51,induced
R3724 T6059 T6058 punct ],51
R3725 T6060 T6033 punct .,induced
R3726 T6062 T6063 nsubjpass Populations,selected
R3727 T6064 T6062 prep of,Populations
R3728 T6065 T6066 nmod C3H10T1,cells
R3729 T6066 T6064 pobj cells,of
R3730 T6067 T6065 punct /,C3H10T1
R3731 T6068 T6065 nummod 2,C3H10T1
R3732 T6069 T6070 advmod stably,transfected
R3733 T6070 T6062 acl transfected,Populations
R3734 T6071 T6070 prep with,transfected
R3735 T6072 T6073 preconj either,retro
R3736 T6073 T6071 pobj retro,with
R3737 T6074 T6073 compound pSuper,retro
R3738 T6075 T6073 punct -,retro
R3739 T6076 T6077 punct (,control
R3740 T6077 T6073 parataxis control,retro
R3741 T6078 T6077 punct ),control
R3742 T6079 T6073 cc and,retro
R3743 T6080 T6081 compound pSuper,retro
R3744 T6081 T6073 conj retro,retro
R3745 T6082 T6081 punct -,retro
R3746 T6083 T6081 acl harboring,retro
R3747 T6084 T6085 det a,hairpin
R3748 T6085 T6083 dobj hairpin,harboring
R3749 T6086 T6085 amod short,hairpin
R3750 T6087 T6085 prep against,hairpin
R3751 T6088 T6087 pobj Sam68,against
R3752 T6089 T6085 punct (,hairpin
R3753 T6090 T6085 appos Sam68sh,hairpin
R3754 T6091 T6063 punct ),selected
R3755 T6092 T6063 auxpass were,selected
R3756 T6093 T6063 prep with,selected
R3757 T6094 T6093 pobj puromycin,with
R3758 T6095 T6063 punct .,selected
R3759 T6097 T6098 det The,expression
R3760 T6098 T6099 nsubjpass expression,reduced
R3761 T6100 T6098 prep of,expression
R3762 T6101 T6100 pobj Sam68,of
R3763 T6102 T6099 auxpass was,reduced
R3764 T6103 T6099 prep by,reduced
R3765 T6104 T6105 advmod approximately,80
R3766 T6105 T6106 nummod 80,%
R3767 T6106 T6103 pobj %,by
R3768 T6107 T6108 mark as,evidenced
R3769 T6108 T6099 advcl evidenced,reduced
R3770 T6109 T6108 agent by,evidenced
R3771 T6110 T6111 compound immunoblot,analyses
R3772 T6111 T6109 pobj analyses,by
R3773 T6112 T6111 acl using,analyses
R3774 T6113 T6114 compound β,actin
R3775 T6114 T6112 dobj actin,using
R3776 T6115 T6114 punct -,actin
R3777 T6116 T6112 prep as,using
R3778 T6117 T6118 det a,control
R3779 T6118 T6116 pobj control,as
R3780 T6119 T6118 compound loading,control
R3781 T6120 T6121 punct (,8A
R3782 T6121 T6099 parataxis 8A,reduced
R3783 T6122 T6121 compound Figure,8A
R3784 T6123 T6121 punct ),8A
R3785 T6124 T6099 punct .,reduced
R3786 T6126 T6127 compound Osteoblast,differentiation
R3787 T6127 T6128 nsubjpass differentiation,induced
R3788 T6129 T6128 auxpass was,induced
R3789 T6130 T6128 prep in,induced
R3790 T6131 T6130 pobj cultures,in
R3791 T6132 T6131 acl expressing,cultures
R3792 T6133 T6134 compound pSuper,retro
R3793 T6134 T6132 dobj retro,expressing
R3794 T6135 T6134 punct -,retro
R3795 T6136 T6134 cc or,retro
R3796 T6137 T6138 compound pSuper,retro
R3797 T6138 T6140 compound retro,Sam68sh
R3798 T6139 T6138 punct -,retro
R3799 T6140 T6134 conj Sam68sh,retro
R3800 T6141 T6128 prep by,induced
R3801 T6142 T6141 pobj addition,by
R3802 T6143 T6142 prep of,addition
R3803 T6144 T6143 pobj BMP,of
R3804 T6145 T6144 punct -,BMP
R3805 T6146 T6144 nummod 2,BMP
R3806 T6147 T6142 prep to,addition
R3807 T6148 T6149 det the,medium
R3808 T6149 T6147 pobj medium,to
R3809 T6150 T6149 compound culture,medium
R3810 T6151 T6128 punct .,induced
R3811 T6152 T6128 punct .,induced
R3812 T6154 T6155 det The,expression
R3813 T6155 T6156 nsubjpass expression,examined
R3814 T6157 T6155 prep of,expression
R3815 T6158 T6157 pobj OCN,of
R3816 T6159 T6158 cc and,OCN
R3817 T6160 T6161 compound β,actin
R3818 T6161 T6163 compound actin,mRNAs
R3819 T6162 T6161 punct -,actin
R3820 T6163 T6158 conj mRNAs,OCN
R3821 T6164 T6156 auxpass was,examined
R3822 T6165 T6156 prep by,examined
R3823 T6166 T6167 amod semiquantitative,PCR
R3824 T6167 T6165 pobj PCR,by
R3825 T6168 T6167 compound RT,PCR
R3826 T6169 T6167 punct -,PCR
R3827 T6170 T6156 cc and,examined
R3828 T6171 T6172 det the,cells
R3829 T6172 T6176 nsubj cells,displayed
R3830 T6173 T6174 npadvmod Sam68sh,expressing
R3831 T6174 T6172 amod expressing,cells
R3832 T6175 T6174 punct -,expressing
R3833 T6176 T6156 conj displayed,examined
R3834 T6177 T6178 det a,phenotype
R3835 T6178 T6176 dobj phenotype,displayed
R3836 T6179 T6180 advmod more,pronounced
R3837 T6180 T6178 amod pronounced,phenotype
R3838 T6181 T6178 compound osteoblast,phenotype
R3839 T6182 T6178 prep compared,phenotype
R3840 T6183 T6182 prep with,compared
R3841 T6184 T6185 compound control,cells
R3842 T6185 T6183 pobj cells,with
R3843 T6186 T6176 punct ", ",displayed
R3844 T6187 T6188 mark as,assessed
R3845 T6188 T6176 advcl assessed,displayed
R3846 T6189 T6188 prep by,assessed
R3847 T6190 T6191 det the,expression
R3848 T6191 T6189 pobj expression,by
R3849 T6192 T6191 prep of,expression
R3850 T6193 T6192 pobj OCN,of
R3851 T6194 T6195 punct (,8B
R3852 T6195 T6176 parataxis 8B,displayed
R3853 T6196 T6195 compound Figure,8B
R3854 T6197 T6195 punct ),8B
R3855 T6198 T6176 punct .,displayed