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PMC:1315279 / 24688-30983 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T5689 0-3 DT denotes The
T5690 4-11 NN denotes Absence
T5692 12-14 IN denotes of
T5693 15-20 NN denotes Sam68
T5691 21-29 VBZ denotes Prevents
T5694 30-39 NN denotes Adipocyte
T5695 40-55 NN denotes Differentiation
T5696 56-59 CC denotes and
T5697 60-68 VBZ denotes Promotes
T5698 69-79 NN denotes Osteoblast
T5699 80-95 NN denotes Differentiation
T5700 95-306 sentence denotes It is well recognized that bone marrow stromal cells give rise to both osteoblasts and adipocytes and that age-related bone loss is accompanied by an increase in differentiation down the adipocyte lineage [22].
T5701 96-98 PRP denotes It
T5703 99-101 VBZ denotes is
T5704 102-106 RB denotes well
T5702 107-117 VBN denotes recognized
T5705 118-122 IN denotes that
T5707 123-127 NN denotes bone
T5708 128-134 NN denotes marrow
T5710 135-142 JJ denotes stromal
T5709 143-148 NNS denotes cells
T5706 149-153 VBP denotes give
T5711 154-158 NN denotes rise
T5712 159-161 IN denotes to
T5713 162-166 DT denotes both
T5714 167-178 NNS denotes osteoblasts
T5715 179-182 CC denotes and
T5716 183-193 NNS denotes adipocytes
T5717 194-197 CC denotes and
T5718 198-202 IN denotes that
T5720 203-206 NN denotes age
T5722 206-207 HYPH denotes -
T5721 207-214 VBN denotes related
T5724 215-219 NN denotes bone
T5723 220-224 NN denotes loss
T5725 225-227 VBZ denotes is
T5719 228-239 VBN denotes accompanied
T5726 240-242 IN denotes by
T5727 243-245 DT denotes an
T5728 246-254 NN denotes increase
T5729 255-257 IN denotes in
T5730 258-273 NN denotes differentiation
T5731 274-278 IN denotes down
T5732 279-282 DT denotes the
T5734 283-292 NN denotes adipocyte
T5733 293-300 NN denotes lineage
T5735 301-302 -LRB- denotes [
T5736 302-304 CD denotes 22
T5737 304-305 -RRB- denotes ]
T5738 305-306 . denotes .
T5739 306-450 sentence denotes Therefore, the loss of Sam68 could influence the bone marrow stromal cells to differentiate along the osteogenic versus the adipogenic pathway.
T5740 307-316 RB denotes Therefore
T5742 316-318 , denotes ,
T5743 318-321 DT denotes the
T5744 322-326 NN denotes loss
T5745 327-329 IN denotes of
T5746 330-335 NN denotes Sam68
T5747 336-341 MD denotes could
T5741 342-351 VB denotes influence
T5748 352-355 DT denotes the
T5750 356-360 NN denotes bone
T5751 361-367 NN denotes marrow
T5752 368-375 JJ denotes stromal
T5749 376-381 NNS denotes cells
T5753 382-384 TO denotes to
T5754 385-398 VB denotes differentiate
T5755 399-404 IN denotes along
T5756 405-408 DT denotes the
T5757 409-419 JJ denotes osteogenic
T5758 420-426 CC denotes versus
T5759 427-430 DT denotes the
T5760 431-441 JJ denotes adipogenic
T5761 442-449 NN denotes pathway
T5762 449-450 . denotes .
T5763 450-684 sentence denotes Alternatively, the loss of Sam68 could indirectly regulate bone mass as a general disturbance of neuroendocrine control as was shown when leptin and the sympathetic nervous system axis were shown to negatively regulate bone mass [8].
T5764 451-464 RB denotes Alternatively
T5766 464-466 , denotes ,
T5767 466-469 DT denotes the
T5768 470-474 NN denotes loss
T5769 475-477 IN denotes of
T5770 478-483 NN denotes Sam68
T5771 484-489 MD denotes could
T5772 490-500 RB denotes indirectly
T5765 501-509 VB denotes regulate
T5773 510-514 NN denotes bone
T5774 515-519 NN denotes mass
T5775 520-522 IN denotes as
T5776 523-524 DT denotes a
T5778 525-532 JJ denotes general
T5777 533-544 NN denotes disturbance
T5779 545-547 IN denotes of
T5780 548-562 JJ denotes neuroendocrine
T5781 563-570 NN denotes control
T5782 571-573 IN denotes as
T5784 574-577 VBD denotes was
T5783 578-583 VBN denotes shown
T5785 584-588 WRB denotes when
T5787 589-595 NN denotes leptin
T5788 596-599 CC denotes and
T5789 600-603 DT denotes the
T5791 604-615 JJ denotes sympathetic
T5792 616-623 JJ denotes nervous
T5793 624-630 NN denotes system
T5790 631-635 NN denotes axis
T5794 636-640 VBD denotes were
T5786 641-646 VBN denotes shown
T5795 647-649 TO denotes to
T5797 650-660 RB denotes negatively
T5796 661-669 VB denotes regulate
T5798 670-674 NN denotes bone
T5799 675-679 NN denotes mass
T5800 680-681 -LRB- denotes [
T5801 681-682 CD denotes 8
T5802 682-683 -RRB- denotes ]
T5803 683-684 . denotes .
T5804 684-834 sentence denotes To confirm a role for Sam68 in the regulation of adipocyte differentiation, we isolated primary MEFs from 14.5-day-old Sam68+/+ and Sam68−/− embryos.
T5805 685-687 TO denotes To
T5806 688-695 VB denotes confirm
T5808 696-697 DT denotes a
T5809 698-702 NN denotes role
T5810 703-706 IN denotes for
T5811 707-712 NN denotes Sam68
T5812 713-715 IN denotes in
T5813 716-719 DT denotes the
T5814 720-730 NN denotes regulation
T5815 731-733 IN denotes of
T5816 734-743 NN denotes adipocyte
T5817 744-759 NN denotes differentiation
T5818 759-761 , denotes ,
T5819 761-763 PRP denotes we
T5807 764-772 VBD denotes isolated
T5820 773-780 JJ denotes primary
T5821 781-785 NNS denotes MEFs
T5822 786-790 IN denotes from
T5823 791-795 CD denotes 14.5
T5825 795-796 HYPH denotes -
T5824 796-799 NN denotes day
T5827 799-800 HYPH denotes -
T5826 800-803 JJ denotes old
T5829 804-809 NN denotes Sam68
T5830 809-810 SYM denotes +
T5831 810-811 HYPH denotes /
T5832 811-812 SYM denotes +
T5833 813-816 CC denotes and
T5834 817-822 NN denotes Sam68
T5835 822-823 SYM denotes −
T5836 823-824 HYPH denotes /
T5837 824-825 SYM denotes −
T5828 826-833 NNS denotes embryos
T5838 833-834 . denotes .
T5839 834-976 sentence denotes The primary Sam68−/− MEFs were differentiated into adipocytes in vitro in culture medium containing 5 μM pioglitazone to induce adipogenesis.
T5840 835-838 DT denotes The
T5842 839-846 JJ denotes primary
T5843 847-852 NN denotes Sam68
T5844 852-853 SYM denotes −
T5845 853-854 HYPH denotes /
T5846 854-855 SYM denotes −
T5841 856-860 NNS denotes MEFs
T5848 861-865 VBD denotes were
T5847 866-880 VBN denotes differentiated
T5849 881-885 IN denotes into
T5850 886-896 NNS denotes adipocytes
T5851 897-899 FW denotes in
T5852 900-905 FW denotes vitro
T5853 906-908 IN denotes in
T5854 909-916 NN denotes culture
T5855 917-923 NN denotes medium
T5856 924-934 VBG denotes containing
T5857 935-936 CD denotes 5
T5858 937-939 NN denotes μM
T5859 940-952 NN denotes pioglitazone
T5860 953-955 TO denotes to
T5861 956-962 VB denotes induce
T5862 963-975 NN denotes adipogenesis
T5863 975-976 . denotes .
T5864 976-1059 sentence denotes Cells at days 0, 4, 6, and 12 were stained with Oil red O to monitor adipogenesis.
T5865 977-982 NNS denotes Cells
T5867 983-985 IN denotes at
T5868 986-990 NNS denotes days
T5869 991-992 CD denotes 0
T5870 992-994 , denotes ,
T5871 994-995 CD denotes 4
T5872 995-997 , denotes ,
T5873 997-998 CD denotes 6
T5874 998-1000 , denotes ,
T5875 1000-1003 CC denotes and
T5876 1004-1006 CD denotes 12
T5877 1007-1011 VBD denotes were
T5866 1012-1019 VBN denotes stained
T5878 1020-1024 IN denotes with
T5879 1025-1028 NN denotes Oil
T5881 1029-1032 NN denotes red
T5880 1033-1034 NN denotes O
T5882 1035-1037 TO denotes to
T5883 1038-1045 VB denotes monitor
T5884 1046-1058 NN denotes adipogenesis
T5885 1058-1059 . denotes .
T5886 1059-1245 sentence denotes Adipogenesis was more pronounced in the wild-type MEFs cultures than in the Sam68−/− MEFs, consistent with the positive role of endogenous Sam68 in adipocyte differentiation (Figure 7).
T5887 1060-1072 NN denotes Adipogenesis
T5888 1073-1076 VBD denotes was
T5889 1077-1081 RBR denotes more
T5890 1082-1092 JJ denotes pronounced
T5891 1093-1095 IN denotes in
T5892 1096-1099 DT denotes the
T5894 1100-1104 JJ denotes wild
T5896 1104-1105 HYPH denotes -
T5895 1105-1109 NN denotes type
T5897 1110-1114 NNS denotes MEFs
T5893 1115-1123 NNS denotes cultures
T5898 1124-1128 IN denotes than
T5899 1129-1131 IN denotes in
T5900 1132-1135 DT denotes the
T5902 1136-1141 NN denotes Sam68
T5903 1141-1142 SYM denotes −
T5904 1142-1143 HYPH denotes /
T5905 1143-1144 SYM denotes −
T5901 1145-1149 NNS denotes MEFs
T5906 1149-1151 , denotes ,
T5907 1151-1161 JJ denotes consistent
T5908 1162-1166 IN denotes with
T5909 1167-1170 DT denotes the
T5911 1171-1179 JJ denotes positive
T5910 1180-1184 NN denotes role
T5912 1185-1187 IN denotes of
T5913 1188-1198 JJ denotes endogenous
T5914 1199-1204 NN denotes Sam68
T5915 1205-1207 IN denotes in
T5916 1208-1217 NN denotes adipocyte
T5917 1218-1233 NN denotes differentiation
T5918 1234-1235 -LRB- denotes (
T5919 1235-1241 NN denotes Figure
T5920 1242-1243 CD denotes 7
T5921 1243-1244 -RRB- denotes )
T5922 1244-1245 . denotes .
T5923 1245-1466 sentence denotes The expression of key transcription factors including the PPARγ and KLF5 was impaired in Sam68−/− differentiated MEFs compared with Sam68+/+ MEFs, consistent with impaired adipogenesis in the absence of Sam68 (Figure 7).
T5924 1246-1249 DT denotes The
T5925 1250-1260 NN denotes expression
T5927 1261-1263 IN denotes of
T5928 1264-1267 JJ denotes key
T5930 1268-1281 NN denotes transcription
T5929 1282-1289 NNS denotes factors
T5931 1290-1299 VBG denotes including
T5932 1300-1303 DT denotes the
T5933 1304-1309 NN denotes PPARγ
T5934 1310-1313 CC denotes and
T5935 1314-1318 NN denotes KLF5
T5936 1319-1322 VBD denotes was
T5926 1323-1331 VBN denotes impaired
T5937 1332-1334 IN denotes in
T5938 1335-1340 NN denotes Sam68
T5940 1340-1341 SYM denotes −
T5941 1341-1342 HYPH denotes /
T5942 1342-1343 SYM denotes −
T5943 1344-1358 VBN denotes differentiated
T5939 1359-1363 NNS denotes MEFs
T5944 1364-1372 VBN denotes compared
T5945 1373-1377 IN denotes with
T5946 1378-1383 NN denotes Sam68
T5948 1383-1384 SYM denotes +
T5949 1384-1385 HYPH denotes /
T5950 1385-1386 SYM denotes +
T5947 1387-1391 NNS denotes MEFs
T5951 1391-1393 , denotes ,
T5952 1393-1403 JJ denotes consistent
T5953 1404-1408 IN denotes with
T5954 1409-1417 VBN denotes impaired
T5955 1418-1430 NN denotes adipogenesis
T5956 1431-1433 IN denotes in
T5957 1434-1437 DT denotes the
T5958 1438-1445 NN denotes absence
T5959 1446-1448 IN denotes of
T5960 1449-1454 NN denotes Sam68
T5961 1455-1456 -LRB- denotes (
T5962 1456-1462 NN denotes Figure
T5963 1463-1464 CD denotes 7
T5964 1464-1465 -RRB- denotes )
T5965 1465-1466 . denotes .
T5966 1466-1674 sentence denotes These data, together with data confirming a lean phenotype in Sam68−/− mice (N. Torabi and S. Richard, unpublished data), support the hypothesis that Sam68 modulates the differentiation of mesenchymal cells.
T5967 1467-1472 DT denotes These
T5968 1473-1477 NNS denotes data
T5970 1477-1479 , denotes ,
T5971 1479-1487 RB denotes together
T5972 1488-1492 IN denotes with
T5973 1493-1497 NNS denotes data
T5974 1498-1508 VBG denotes confirming
T5975 1509-1510 DT denotes a
T5977 1511-1515 JJ denotes lean
T5976 1516-1525 NN denotes phenotype
T5978 1526-1528 IN denotes in
T5979 1529-1534 NN denotes Sam68
T5981 1534-1535 SYM denotes −
T5982 1535-1536 HYPH denotes /
T5983 1536-1537 SYM denotes −
T5980 1538-1542 NNS denotes mice
T5984 1543-1544 -LRB- denotes (
T5985 1544-1546 NNP denotes N.
T5986 1547-1553 NNP denotes Torabi
T5987 1554-1557 CC denotes and
T5988 1558-1560 NNP denotes S.
T5989 1561-1568 NNP denotes Richard
T5990 1568-1570 , denotes ,
T5991 1570-1581 JJ denotes unpublished
T5992 1582-1586 NNS denotes data
T5993 1586-1587 -RRB- denotes )
T5994 1587-1589 , denotes ,
T5969 1589-1596 VBP denotes support
T5995 1597-1600 DT denotes the
T5996 1601-1611 NN denotes hypothesis
T5997 1612-1616 IN denotes that
T5999 1617-1622 NN denotes Sam68
T5998 1623-1632 VBZ denotes modulates
T6000 1633-1636 DT denotes the
T6001 1637-1652 NN denotes differentiation
T6002 1653-1655 IN denotes of
T6003 1656-1667 JJ denotes mesenchymal
T6004 1668-1673 NNS denotes cells
T6005 1673-1674 . denotes .
T6006 1674-2740 sentence denotes Figure 7 Ex Vivo Adipogenesis Analysis of Sam68−/− Mouse Embryonic Fibroblasts MEFs were isolated from mouse embryos at embryonic day 14.5. Equal number of MEFs from Sam68+/+ and Sam68−/− was plated on glass cover slips in 24 well-plates. Adipocyte differentiation was carried out at indicated times by the addition of complete media containing the pioglitazone. (A) Cultures were fixed in 4% paraformaldehyde and stained with Oil Red O to detect the fat droplets stored in adipocytes and photographed (top). The cell images were magnified ×10 and ×20 as indicated. (B) RT-PCR was carried out on total cellular RNA isolated after differentiation of the MEFs for day 0, 2, 4, 6, and 12. The DNA fragments were visualized on agarose gels stained with ethidium bromide. The expression of adipogenic markers C/EBPβ, C/EBPδ, PPARα, and KLF5 was examined as well as the expression of controls including Sam68, β-actin, and GAPDH. To further examine this phenotype in a cell autonomous system, we chose the embryonic mesenchymal multipotential progenitor cells C3H10T1/2.
T6007 2600-2602 TO denotes To
T6009 2603-2610 RB denotes further
T6008 2611-2618 VB denotes examine
T6011 2619-2623 DT denotes this
T6012 2624-2633 NN denotes phenotype
T6013 2634-2636 IN denotes in
T6014 2637-2638 DT denotes a
T6016 2639-2643 NN denotes cell
T6017 2644-2654 JJ denotes autonomous
T6015 2655-2661 NN denotes system
T6018 2661-2663 , denotes ,
T6019 2663-2665 PRP denotes we
T6010 2666-2671 VBD denotes chose
T6020 2672-2675 DT denotes the
T6022 2676-2685 JJ denotes embryonic
T6023 2686-2697 JJ denotes mesenchymal
T6024 2698-2712 JJ denotes multipotential
T6025 2713-2723 NN denotes progenitor
T6021 2724-2729 NNS denotes cells
T6026 2730-2737 NN denotes C3H10T1
T6027 2737-2738 HYPH denotes /
T6028 2738-2739 CD denotes 2
T6029 2739-2740 . denotes .
T6030 2740-2899 sentence denotes The addition of BMP-2 induced osteoblast differentiation, as evidenced by an approximately 400-fold increase in expression of the osteocalcin (OCN) gene [51].
T6031 2741-2744 DT denotes The
T6032 2745-2753 NN denotes addition
T6034 2754-2756 IN denotes of
T6035 2757-2760 NN denotes BMP
T6036 2760-2761 HYPH denotes -
T6037 2761-2762 CD denotes 2
T6033 2763-2770 VBD denotes induced
T6038 2771-2781 NN denotes osteoblast
T6039 2782-2797 NN denotes differentiation
T6040 2797-2799 , denotes ,
T6041 2799-2801 IN denotes as
T6042 2802-2811 VBN denotes evidenced
T6043 2812-2814 IN denotes by
T6044 2815-2817 DT denotes an
T6046 2818-2831 RB denotes approximately
T6047 2832-2840 JJ denotes 400-fold
T6045 2841-2849 NN denotes increase
T6048 2850-2852 IN denotes in
T6049 2853-2863 NN denotes expression
T6050 2864-2866 IN denotes of
T6051 2867-2870 DT denotes the
T6053 2871-2882 NN denotes osteocalcin
T6054 2883-2884 -LRB- denotes (
T6055 2884-2887 NN denotes OCN
T6056 2887-2888 -RRB- denotes )
T6052 2889-2893 NN denotes gene
T6057 2894-2895 -LRB- denotes [
T6058 2895-2897 CD denotes 51
T6059 2897-2898 -RRB- denotes ]
T6060 2898-2899 . denotes .
T6061 2899-3081 sentence denotes Populations of C3H10T1/2 cells stably transfected with either pSuper-retro (control) and pSuper-retro harboring a short hairpin against Sam68 (Sam68sh) were selected with puromycin.
T6062 2900-2911 NNS denotes Populations
T6064 2912-2914 IN denotes of
T6065 2915-2922 NN denotes C3H10T1
T6067 2922-2923 HYPH denotes /
T6068 2923-2924 CD denotes 2
T6066 2925-2930 NNS denotes cells
T6069 2931-2937 RB denotes stably
T6070 2938-2949 VBN denotes transfected
T6071 2950-2954 IN denotes with
T6072 2955-2961 CC denotes either
T6074 2962-2968 NN denotes pSuper
T6075 2968-2969 HYPH denotes -
T6073 2969-2974 NN denotes retro
T6076 2975-2976 -LRB- denotes (
T6077 2976-2983 NN denotes control
T6078 2983-2984 -RRB- denotes )
T6079 2985-2988 CC denotes and
T6080 2989-2995 NN denotes pSuper
T6082 2995-2996 HYPH denotes -
T6081 2996-3001 NN denotes retro
T6083 3002-3011 VBG denotes harboring
T6084 3012-3013 DT denotes a
T6086 3014-3019 JJ denotes short
T6085 3020-3027 NN denotes hairpin
T6087 3028-3035 IN denotes against
T6088 3036-3041 NN denotes Sam68
T6089 3042-3043 -LRB- denotes (
T6090 3043-3050 NN denotes Sam68sh
T6091 3050-3051 -RRB- denotes )
T6092 3052-3056 VBD denotes were
T6063 3057-3065 VBN denotes selected
T6093 3066-3070 IN denotes with
T6094 3071-3080 NN denotes puromycin
T6095 3080-3081 . denotes .
T6096 3081-3222 sentence denotes The expression of Sam68 was reduced by approximately 80% as evidenced by immunoblot analyses using β-actin as a loading control (Figure 8A).
T6097 3082-3085 DT denotes The
T6098 3086-3096 NN denotes expression
T6100 3097-3099 IN denotes of
T6101 3100-3105 NN denotes Sam68
T6102 3106-3109 VBD denotes was
T6099 3110-3117 VBN denotes reduced
T6103 3118-3120 IN denotes by
T6104 3121-3134 RB denotes approximately
T6105 3135-3137 CD denotes 80
T6106 3137-3138 NN denotes %
T6107 3139-3141 IN denotes as
T6108 3142-3151 VBN denotes evidenced
T6109 3152-3154 IN denotes by
T6110 3155-3165 NN denotes immunoblot
T6111 3166-3174 NNS denotes analyses
T6112 3175-3180 VBG denotes using
T6113 3181-3182 NN denotes β
T6115 3182-3183 HYPH denotes -
T6114 3183-3188 NN denotes actin
T6116 3189-3191 IN denotes as
T6117 3192-3193 DT denotes a
T6119 3194-3201 NN denotes loading
T6118 3202-3209 NN denotes control
T6120 3210-3211 -LRB- denotes (
T6122 3211-3217 NN denotes Figure
T6121 3218-3220 NN denotes 8A
T6123 3220-3221 -RRB- denotes )
T6124 3221-3222 . denotes .
T6125 3222-3367 sentence denotes Osteoblast differentiation was induced in cultures expressing pSuper-retro or pSuper-retro Sam68sh by addition of BMP-2 to the culture medium. .
T6126 3223-3233 NN denotes Osteoblast
T6127 3234-3249 NN denotes differentiation
T6129 3250-3253 VBD denotes was
T6128 3254-3261 VBN denotes induced
T6130 3262-3264 IN denotes in
T6131 3265-3273 NNS denotes cultures
T6132 3274-3284 VBG denotes expressing
T6133 3285-3291 NN denotes pSuper
T6135 3291-3292 HYPH denotes -
T6134 3292-3297 NN denotes retro
T6136 3298-3300 CC denotes or
T6137 3301-3307 NN denotes pSuper
T6139 3307-3308 HYPH denotes -
T6138 3308-3313 NN denotes retro
T6140 3314-3321 NN denotes Sam68sh
T6141 3322-3324 IN denotes by
T6142 3325-3333 NN denotes addition
T6143 3334-3336 IN denotes of
T6144 3337-3340 NN denotes BMP
T6145 3340-3341 HYPH denotes -
T6146 3341-3342 CD denotes 2
T6147 3343-3345 IN denotes to
T6148 3346-3349 DT denotes the
T6150 3350-3357 NN denotes culture
T6149 3358-3364 NN denotes medium
T6151 3364-3365 . denotes .
T6152 3366-3367 . denotes .
T6153 3367-3608 sentence denotes The expression of OCN and β-actin mRNAs was examined by semiquantitative RT-PCR and the Sam68sh-expressing cells displayed a more pronounced osteoblast phenotype compared with control cells, as assessed by the expression of OCN (Figure 8B).
T6154 3368-3371 DT denotes The
T6155 3372-3382 NN denotes expression
T6157 3383-3385 IN denotes of
T6158 3386-3389 NN denotes OCN
T6159 3390-3393 CC denotes and
T6160 3394-3395 NN denotes β
T6162 3395-3396 HYPH denotes -
T6161 3396-3401 NN denotes actin
T6163 3402-3407 NNS denotes mRNAs
T6164 3408-3411 VBD denotes was
T6156 3412-3420 VBN denotes examined
T6165 3421-3423 IN denotes by
T6166 3424-3440 JJ denotes semiquantitative
T6168 3441-3443 NN denotes RT
T6169 3443-3444 HYPH denotes -
T6167 3444-3447 NN denotes PCR
T6170 3448-3451 CC denotes and
T6171 3452-3455 DT denotes the
T6173 3456-3463 NN denotes Sam68sh
T6175 3463-3464 HYPH denotes -
T6174 3464-3474 VBG denotes expressing
T6172 3475-3480 NNS denotes cells
T6176 3481-3490 VBD denotes displayed
T6177 3491-3492 DT denotes a
T6179 3493-3497 RBR denotes more
T6180 3498-3508 JJ denotes pronounced
T6181 3509-3519 NN denotes osteoblast
T6178 3520-3529 NN denotes phenotype
T6182 3530-3538 VBN denotes compared
T6183 3539-3543 IN denotes with
T6184 3544-3551 NN denotes control
T6185 3552-3557 NNS denotes cells
T6186 3557-3559 , denotes ,
T6187 3559-3561 IN denotes as
T6188 3562-3570 VBN denotes assessed
T6189 3571-3573 IN denotes by
T6190 3574-3577 DT denotes the
T6191 3578-3588 NN denotes expression
T6192 3589-3591 IN denotes of
T6193 3592-3595 NN denotes OCN
T6194 3596-3597 -LRB- denotes (
T6196 3597-3603 NN denotes Figure
T6195 3604-3606 NN denotes 8B
T6197 3606-3607 -RRB- denotes )
T6198 3607-3608 . denotes .
T6199 3608-4741 sentence denotes Figure 8 Enhanced Osteogenic Differentiation of the C3HT101/2 Embryonic Cell Line Depleted of Endogenous Sam68 (A) C3HT101/2 cells transfected with an empty vector (pSuper-retro) or a vector containing an shRNA (Sam68 shRNA) were selected with puromycin, and knockdown populations depleted of Sam68 were identified. The reduction in Sam68 protein was analyzed by immunoblotting with anti-Sam68 (AD1) antibody and anti–β-actin antibodies as loading controls. (B) Osteogenic differentiation was carried out with conditioned medium containing BMP-2 for the indicated times. To assess the level of osteogenic differentiation in these cells, expression of late osteoblast marker, osteocalcin (OCN), was analyzed by RT-PCR and compared with β-actin and GAPDH controls. The DNA fragments were visualized by agarose gel stained with ethidium bromide. Given the apparent enhancement of mineralized nodule formation by Sam68−/− bone marrow stromal cells ex vivo and the phenotype observed with short hairpin RNA (shRNA)-treated C3H10T1/2, we stained sections of bone from 4- and 12-month-old mice for evidence of changes in marrow adiposity.
T6200 4453-4458 VBN denotes Given
T6202 4459-4462 DT denotes the
T6204 4463-4471 JJ denotes apparent
T6203 4472-4483 NN denotes enhancement
T6205 4484-4486 IN denotes of
T6206 4487-4498 VBN denotes mineralized
T6207 4499-4505 NN denotes nodule
T6208 4506-4515 NN denotes formation
T6209 4516-4518 IN denotes by
T6210 4519-4524 NN denotes Sam68
T6212 4524-4525 SYM denotes −
T6213 4525-4526 HYPH denotes /
T6214 4526-4527 SYM denotes −
T6215 4528-4532 NN denotes bone
T6216 4533-4539 NN denotes marrow
T6217 4540-4547 JJ denotes stromal
T6211 4548-4553 NNS denotes cells
T6218 4554-4556 FW denotes ex
T6219 4557-4561 FW denotes vivo
T6220 4562-4565 CC denotes and
T6221 4566-4569 DT denotes the
T6222 4570-4579 NN denotes phenotype
T6223 4580-4588 VBN denotes observed
T6224 4589-4593 IN denotes with
T6225 4594-4599 JJ denotes short
T6227 4600-4607 NN denotes hairpin
T6226 4608-4611 NN denotes RNA
T6229 4612-4613 -LRB- denotes (
T6230 4613-4618 NN denotes shRNA
T6231 4618-4619 -RRB- denotes )
T6232 4619-4620 HYPH denotes -
T6228 4620-4627 VBN denotes treated
T6233 4628-4635 NN denotes C3H10T1
T6234 4635-4636 HYPH denotes /
T6235 4636-4637 CD denotes 2
T6236 4637-4639 , denotes ,
T6237 4639-4641 PRP denotes we
T6201 4642-4649 VBD denotes stained
T6238 4650-4658 NNS denotes sections
T6239 4659-4661 IN denotes of
T6240 4662-4666 NN denotes bone
T6241 4667-4671 IN denotes from
T6242 4672-4673 CD denotes 4
T6244 4673-4674 HYPH denotes -
T6245 4675-4678 CC denotes and
T6246 4679-4681 CD denotes 12
T6247 4681-4682 HYPH denotes -
T6243 4682-4687 NN denotes month
T6249 4687-4688 HYPH denotes -
T6248 4688-4691 JJ denotes old
T6250 4692-4696 NNS denotes mice
T6251 4697-4700 IN denotes for
T6252 4701-4709 NN denotes evidence
T6253 4710-4712 IN denotes of
T6254 4713-4720 NNS denotes changes
T6255 4721-4723 IN denotes in
T6256 4724-4730 NN denotes marrow
T6257 4731-4740 NN denotes adiposity
T6258 4740-4741 . denotes .
T6259 4741-5059 sentence denotes Figure 9 shows sections of undecalcified bone from 4- and 12-month-old Sam68+/+ and Sam68−/− mice stained with von Kossa and toluidine blue to show mineralized tissue (Figure 9A, black) and marrow adipocytes (Figure 9B, white) and left unstained to show fluorochrome labeling of the mineralization fronts (Figure 9C).
T6260 4742-4748 NN denotes Figure
T6262 4749-4750 CD denotes 9
T6261 4751-4756 VBZ denotes shows
T6263 4757-4765 NNS denotes sections
T6264 4766-4768 IN denotes of
T6265 4769-4782 JJ denotes undecalcified
T6266 4783-4787 NN denotes bone
T6267 4788-4792 IN denotes from
T6268 4793-4794 CD denotes 4
T6270 4794-4795 HYPH denotes -
T6271 4796-4799 CC denotes and
T6272 4800-4802 CD denotes 12
T6273 4802-4803 HYPH denotes -
T6269 4803-4808 NN denotes month
T6275 4808-4809 HYPH denotes -
T6274 4809-4812 JJ denotes old
T6277 4813-4818 NN denotes Sam68
T6278 4818-4819 SYM denotes +
T6279 4819-4820 HYPH denotes /
T6280 4820-4821 SYM denotes +
T6281 4822-4825 CC denotes and
T6282 4826-4831 NN denotes Sam68
T6283 4831-4832 SYM denotes −
T6284 4832-4833 HYPH denotes /
T6285 4833-4834 SYM denotes −
T6276 4835-4839 NNS denotes mice
T6286 4840-4847 VBN denotes stained
T6287 4848-4852 IN denotes with
T6288 4853-4856 NNP denotes von
T6289 4857-4862 NNP denotes Kossa
T6290 4863-4866 CC denotes and
T6291 4867-4876 NN denotes toluidine
T6292 4877-4881 JJ denotes blue
T6293 4882-4884 TO denotes to
T6294 4885-4889 VB denotes show
T6295 4890-4901 VBN denotes mineralized
T6296 4902-4908 NN denotes tissue
T6297 4909-4910 -LRB- denotes (
T6299 4910-4916 NN denotes Figure
T6298 4917-4919 NN denotes 9A
T6300 4919-4921 , denotes ,
T6301 4921-4926 JJ denotes black
T6302 4926-4927 -RRB- denotes )
T6303 4928-4931 CC denotes and
T6304 4932-4938 NN denotes marrow
T6305 4939-4949 NNS denotes adipocytes
T6306 4950-4951 -LRB- denotes (
T6308 4951-4957 NN denotes Figure
T6307 4958-4960 NN denotes 9B
T6309 4960-4962 , denotes ,
T6310 4962-4967 JJ denotes white
T6311 4967-4968 -RRB- denotes )
T6312 4969-4972 CC denotes and
T6313 4973-4977 VBD denotes left
T6314 4978-4987 JJ denotes unstained
T6315 4988-4990 TO denotes to
T6316 4991-4995 VB denotes show
T6317 4996-5008 NN denotes fluorochrome
T6318 5009-5017 NN denotes labeling
T6319 5018-5020 IN denotes of
T6320 5021-5024 DT denotes the
T6322 5025-5039 NN denotes mineralization
T6321 5040-5046 NNS denotes fronts
T6323 5047-5048 -LRB- denotes (
T6325 5048-5054 NN denotes Figure
T6324 5055-5057 NN denotes 9C
T6326 5057-5058 -RRB- denotes )
T6327 5058-5059 . denotes .
T6328 5059-5312 sentence denotes Twelve-month-old Sam68+/+ mice showed a noticeable decrease in trabecular bone (Figure 9A), which was associated with a significant increase in marrow adiposity (Figure 9B) and with the two fluorochrome labels superimposed upon one another (Figure 9C).
T6329 5060-5066 CD denotes Twelve
T6331 5066-5067 HYPH denotes -
T6330 5067-5072 NN denotes month
T6333 5072-5073 HYPH denotes -
T6332 5073-5076 JJ denotes old
T6335 5077-5082 NN denotes Sam68
T6336 5082-5083 SYM denotes +
T6337 5083-5084 HYPH denotes /
T6338 5084-5085 SYM denotes +
T6334 5086-5090 NNS denotes mice
T6339 5091-5097 VBD denotes showed
T6340 5098-5099 DT denotes a
T6342 5100-5110 JJ denotes noticeable
T6341 5111-5119 NN denotes decrease
T6343 5120-5122 IN denotes in
T6344 5123-5133 JJ denotes trabecular
T6345 5134-5138 NN denotes bone
T6346 5139-5140 -LRB- denotes (
T6348 5140-5146 NN denotes Figure
T6347 5147-5149 NN denotes 9A
T6349 5149-5150 -RRB- denotes )
T6350 5150-5152 , denotes ,
T6351 5152-5157 WDT denotes which
T6353 5158-5161 VBD denotes was
T6352 5162-5172 VBN denotes associated
T6354 5173-5177 IN denotes with
T6355 5178-5179 DT denotes a
T6357 5180-5191 JJ denotes significant
T6356 5192-5200 NN denotes increase
T6358 5201-5203 IN denotes in
T6359 5204-5210 NN denotes marrow
T6360 5211-5220 NN denotes adiposity
T6361 5221-5222 -LRB- denotes (
T6363 5222-5228 NN denotes Figure
T6362 5229-5231 NN denotes 9B
T6364 5231-5232 -RRB- denotes )
T6365 5233-5236 CC denotes and
T6366 5237-5241 IN denotes with
T6367 5242-5245 DT denotes the
T6369 5246-5249 CD denotes two
T6370 5250-5262 NN denotes fluorochrome
T6368 5263-5269 NNS denotes labels
T6371 5270-5282 VBN denotes superimposed
T6372 5283-5287 IN denotes upon
T6373 5288-5291 CD denotes one
T6374 5292-5299 DT denotes another
T6375 5300-5301 -LRB- denotes (
T6377 5301-5307 NN denotes Figure
T6376 5308-5310 NN denotes 9C
T6378 5310-5311 -RRB- denotes )
T6379 5311-5312 . denotes .
T6380 5312-5435 sentence denotes In contrast, the bones of 12-month-old Sam68−/− mice appeared similar to those of the 4-month-old mice of either genotype.
T6381 5313-5315 IN denotes In
T6383 5316-5324 NN denotes contrast
T6384 5324-5326 , denotes ,
T6385 5326-5329 DT denotes the
T6386 5330-5335 NNS denotes bones
T6387 5336-5338 IN denotes of
T6388 5339-5341 CD denotes 12
T6390 5341-5342 HYPH denotes -
T6389 5342-5347 NN denotes month
T6392 5347-5348 HYPH denotes -
T6391 5348-5351 JJ denotes old
T6394 5352-5357 NN denotes Sam68
T6395 5357-5358 SYM denotes −
T6396 5358-5359 HYPH denotes /
T6397 5359-5360 SYM denotes −
T6393 5361-5365 NNS denotes mice
T6382 5366-5374 VBD denotes appeared
T6398 5375-5382 JJ denotes similar
T6399 5383-5385 IN denotes to
T6400 5386-5391 DT denotes those
T6401 5392-5394 IN denotes of
T6402 5395-5398 DT denotes the
T6404 5399-5400 CD denotes 4
T6406 5400-5401 HYPH denotes -
T6405 5401-5406 NN denotes month
T6408 5406-5407 HYPH denotes -
T6407 5407-5410 JJ denotes old
T6403 5411-5415 NNS denotes mice
T6409 5416-5418 IN denotes of
T6410 5419-5425 DT denotes either
T6411 5426-5434 NN denotes genotype
T6412 5434-5435 . denotes .
T6413 5435-5623 sentence denotes These data demonstrate that Sam68 regulates the differentiation of bone marrow mesenchymal cells to promote adipocyte differentiation and inhibit osteoblast differentiation in aging bone.
T6414 5436-5441 DT denotes These
T6415 5442-5446 NNS denotes data
T6416 5447-5458 VBP denotes demonstrate
T6417 5459-5463 IN denotes that
T6419 5464-5469 NN denotes Sam68
T6418 5470-5479 VBZ denotes regulates
T6420 5480-5483 DT denotes the
T6421 5484-5499 NN denotes differentiation
T6422 5500-5502 IN denotes of
T6423 5503-5507 NN denotes bone
T6424 5508-5514 NN denotes marrow
T6426 5515-5526 JJ denotes mesenchymal
T6425 5527-5532 NNS denotes cells
T6427 5533-5535 TO denotes to
T6428 5536-5543 VB denotes promote
T6429 5544-5553 NN denotes adipocyte
T6430 5554-5569 NN denotes differentiation
T6431 5570-5573 CC denotes and
T6432 5574-5581 VB denotes inhibit
T6433 5582-5592 NN denotes osteoblast
T6434 5593-5608 NN denotes differentiation
T6435 5609-5611 IN denotes in
T6436 5612-5617 VBG denotes aging
T6437 5618-5622 NN denotes bone
T6438 5622-5623 . denotes .
R3377 T5689 T5690 det The,Absence
R3378 T5690 T5691 nsubj Absence,Prevents
R3379 T5692 T5690 prep of,Absence
R3380 T5693 T5692 pobj Sam68,of
R3381 T5694 T5695 compound Adipocyte,Differentiation
R3382 T5695 T5691 dobj Differentiation,Prevents
R3383 T5696 T5691 cc and,Prevents
R3384 T5697 T5691 conj Promotes,Prevents
R3385 T5698 T5699 compound Osteoblast,Differentiation
R3386 T5699 T5697 dobj Differentiation,Promotes
R3387 T5701 T5702 nsubjpass It,recognized
R3388 T5703 T5702 auxpass is,recognized
R3389 T5704 T5702 advmod well,recognized
R3390 T5705 T5706 mark that,give
R3391 T5706 T5702 advcl give,recognized
R3392 T5707 T5708 nmod bone,marrow
R3393 T5708 T5709 nmod marrow,cells
R3394 T5709 T5706 nsubj cells,give
R3395 T5710 T5709 amod stromal,cells
R3396 T5711 T5706 dobj rise,give
R3397 T5712 T5706 prep to,give
R3398 T5713 T5714 det both,osteoblasts
R3399 T5714 T5712 pobj osteoblasts,to
R3400 T5715 T5714 cc and,osteoblasts
R3401 T5716 T5714 conj adipocytes,osteoblasts
R3402 T5717 T5706 cc and,give
R3403 T5718 T5719 mark that,accompanied
R3404 T5719 T5706 conj accompanied,give
R3405 T5720 T5721 npadvmod age,related
R3406 T5721 T5723 amod related,loss
R3407 T5722 T5721 punct -,related
R3408 T5723 T5719 nsubjpass loss,accompanied
R3409 T5724 T5723 compound bone,loss
R3410 T5725 T5719 auxpass is,accompanied
R3411 T5726 T5719 agent by,accompanied
R3412 T5727 T5728 det an,increase
R3413 T5728 T5726 pobj increase,by
R3414 T5729 T5728 prep in,increase
R3415 T5730 T5729 pobj differentiation,in
R3416 T5731 T5719 prep down,accompanied
R3417 T5732 T5733 det the,lineage
R3418 T5733 T5731 pobj lineage,down
R3419 T5734 T5733 compound adipocyte,lineage
R3420 T5735 T5736 punct [,22
R3421 T5736 T5719 parataxis 22,accompanied
R3422 T5737 T5736 punct ],22
R3423 T5738 T5702 punct .,recognized
R3424 T5740 T5741 advmod Therefore,influence
R3425 T5742 T5741 punct ", ",influence
R3426 T5743 T5744 det the,loss
R3427 T5744 T5741 nsubj loss,influence
R3428 T5745 T5744 prep of,loss
R3429 T5746 T5745 pobj Sam68,of
R3430 T5747 T5741 aux could,influence
R3431 T5748 T5749 det the,cells
R3432 T5749 T5741 dobj cells,influence
R3433 T5750 T5751 nmod bone,marrow
R3434 T5751 T5749 nmod marrow,cells
R3435 T5752 T5749 amod stromal,cells
R3436 T5753 T5754 aux to,differentiate
R3437 T5754 T5741 advcl differentiate,influence
R3438 T5755 T5754 prep along,differentiate
R3439 T5756 T5757 det the,osteogenic
R3440 T5757 T5755 pobj osteogenic,along
R3441 T5758 T5757 cc versus,osteogenic
R3442 T5759 T5760 det the,adipogenic
R3443 T5760 T5761 nmod adipogenic,pathway
R3444 T5761 T5757 conj pathway,osteogenic
R3445 T5762 T5741 punct .,influence
R3446 T5764 T5765 advmod Alternatively,regulate
R3447 T5766 T5765 punct ", ",regulate
R3448 T5767 T5768 det the,loss
R3449 T5768 T5765 nsubj loss,regulate
R3450 T5769 T5768 prep of,loss
R3451 T5770 T5769 pobj Sam68,of
R3452 T5771 T5765 aux could,regulate
R3453 T5772 T5765 advmod indirectly,regulate
R3454 T5773 T5774 compound bone,mass
R3455 T5774 T5765 dobj mass,regulate
R3456 T5775 T5765 prep as,regulate
R3457 T5776 T5777 det a,disturbance
R3458 T5777 T5775 pobj disturbance,as
R3459 T5778 T5777 amod general,disturbance
R3460 T5779 T5777 prep of,disturbance
R3461 T5780 T5781 amod neuroendocrine,control
R3462 T5781 T5779 pobj control,of
R3463 T5782 T5783 mark as,shown
R3464 T5783 T5765 advcl shown,regulate
R3465 T5784 T5783 auxpass was,shown
R3466 T5785 T5786 advmod when,shown
R3467 T5786 T5783 advcl shown,shown
R3468 T5787 T5786 nsubjpass leptin,shown
R3469 T5788 T5787 cc and,leptin
R3470 T5789 T5790 det the,axis
R3471 T5790 T5787 conj axis,leptin
R3472 T5791 T5790 amod sympathetic,axis
R3473 T5792 T5793 amod nervous,system
R3474 T5793 T5790 compound system,axis
R3475 T5794 T5786 auxpass were,shown
R3476 T5795 T5796 aux to,regulate
R3477 T5796 T5786 xcomp regulate,shown
R3478 T5797 T5796 advmod negatively,regulate
R3479 T5798 T5799 compound bone,mass
R3480 T5799 T5796 dobj mass,regulate
R3481 T5800 T5801 punct [,8
R3482 T5801 T5765 parataxis 8,regulate
R3483 T5802 T5801 punct ],8
R3484 T5803 T5765 punct .,regulate
R3485 T5805 T5806 aux To,confirm
R3486 T5806 T5807 advcl confirm,isolated
R3487 T5808 T5809 det a,role
R3488 T5809 T5806 dobj role,confirm
R3489 T5810 T5809 prep for,role
R3490 T5811 T5810 pobj Sam68,for
R3491 T5812 T5809 prep in,role
R3492 T5813 T5814 det the,regulation
R3493 T5814 T5812 pobj regulation,in
R3494 T5815 T5814 prep of,regulation
R3495 T5816 T5817 compound adipocyte,differentiation
R3496 T5817 T5815 pobj differentiation,of
R3497 T5818 T5807 punct ", ",isolated
R3498 T5819 T5807 nsubj we,isolated
R3499 T5820 T5821 amod primary,MEFs
R3500 T5821 T5807 dobj MEFs,isolated
R3501 T5822 T5821 prep from,MEFs
R3502 T5823 T5824 nummod 14.5,day
R3503 T5824 T5826 npadvmod day,old
R3504 T5825 T5824 punct -,day
R3505 T5826 T5828 amod old,embryos
R3506 T5827 T5826 punct -,old
R3507 T5828 T5822 pobj embryos,from
R3508 T5829 T5828 nmod Sam68,embryos
R3509 T5830 T5829 punct +,Sam68
R3510 T5831 T5829 punct /,Sam68
R3511 T5832 T5829 punct +,Sam68
R3512 T5833 T5829 cc and,Sam68
R3513 T5834 T5829 conj Sam68,Sam68
R3514 T5835 T5834 punct −,Sam68
R3515 T5836 T5834 punct /,Sam68
R3516 T5837 T5834 punct −,Sam68
R3517 T5838 T5807 punct .,isolated
R3518 T5840 T5841 det The,MEFs
R3519 T5841 T5847 nsubjpass MEFs,differentiated
R3520 T5842 T5841 amod primary,MEFs
R3521 T5843 T5841 nmod Sam68,MEFs
R3522 T5844 T5843 punct −,Sam68
R3523 T5845 T5843 punct /,Sam68
R3524 T5846 T5843 punct −,Sam68
R3525 T5848 T5847 auxpass were,differentiated
R3526 T5849 T5847 prep into,differentiated
R3527 T5850 T5849 pobj adipocytes,into
R3528 T5851 T5852 advmod in,vitro
R3529 T5852 T5847 advmod vitro,differentiated
R3530 T5853 T5847 prep in,differentiated
R3531 T5854 T5855 compound culture,medium
R3532 T5855 T5853 pobj medium,in
R3533 T5856 T5855 acl containing,medium
R3534 T5857 T5858 nummod 5,μM
R3535 T5858 T5859 compound μM,pioglitazone
R3536 T5859 T5856 dobj pioglitazone,containing
R3537 T5860 T5861 aux to,induce
R3538 T5861 T5847 advcl induce,differentiated
R3539 T5862 T5861 dobj adipogenesis,induce
R3540 T5863 T5847 punct .,differentiated
R3541 T5865 T5866 nsubjpass Cells,stained
R3542 T5867 T5865 prep at,Cells
R3543 T5868 T5869 nmod days,0
R3544 T5869 T5867 pobj 0,at
R3545 T5870 T5869 punct ", ",0
R3546 T5871 T5869 conj 4,0
R3547 T5872 T5871 punct ", ",4
R3548 T5873 T5871 conj 6,4
R3549 T5874 T5873 punct ", ",6
R3550 T5875 T5873 cc and,6
R3551 T5876 T5873 conj 12,6
R3552 T5877 T5866 auxpass were,stained
R3553 T5878 T5866 prep with,stained
R3554 T5879 T5880 compound Oil,O
R3555 T5880 T5878 pobj O,with
R3556 T5881 T5880 compound red,O
R3557 T5882 T5883 aux to,monitor
R3558 T5883 T5866 advcl monitor,stained
R3559 T5884 T5883 dobj adipogenesis,monitor
R3560 T5885 T5866 punct .,stained
R3561 T5887 T5888 nsubj Adipogenesis,was
R3562 T5889 T5890 advmod more,pronounced
R3563 T5890 T5888 acomp pronounced,was
R3564 T5891 T5888 prep in,was
R3565 T5892 T5893 det the,cultures
R3566 T5893 T5891 pobj cultures,in
R3567 T5894 T5895 amod wild,type
R3568 T5895 T5893 compound type,cultures
R3569 T5896 T5895 punct -,type
R3570 T5897 T5893 compound MEFs,cultures
R3571 T5898 T5888 prep than,was
R3572 T5899 T5898 prep in,than
R3573 T5900 T5901 det the,MEFs
R3574 T5901 T5899 pobj MEFs,in
R3575 T5902 T5901 nmod Sam68,MEFs
R3576 T5903 T5902 punct −,Sam68
R3577 T5904 T5902 punct /,Sam68
R3578 T5905 T5902 punct −,Sam68
R3579 T5906 T5888 punct ", ",was
R3580 T5907 T5888 advcl consistent,was
R3581 T5908 T5907 prep with,consistent
R3582 T5909 T5910 det the,role
R3583 T5910 T5908 pobj role,with
R3584 T5911 T5910 amod positive,role
R3585 T5912 T5910 prep of,role
R3586 T5913 T5914 amod endogenous,Sam68
R3587 T5914 T5912 pobj Sam68,of
R3588 T5915 T5910 prep in,role
R3589 T5916 T5917 compound adipocyte,differentiation
R3590 T5917 T5915 pobj differentiation,in
R3591 T5918 T5919 punct (,Figure
R3592 T5919 T5888 parataxis Figure,was
R3593 T5920 T5919 nummod 7,Figure
R3594 T5921 T5919 punct ),Figure
R3595 T5922 T5888 punct .,was
R3596 T5924 T5925 det The,expression
R3597 T5925 T5926 nsubjpass expression,impaired
R3598 T5927 T5925 prep of,expression
R3599 T5928 T5929 amod key,factors
R3600 T5929 T5927 pobj factors,of
R3601 T5930 T5929 compound transcription,factors
R3602 T5931 T5929 prep including,factors
R3603 T5932 T5933 det the,PPARγ
R3604 T5933 T5931 pobj PPARγ,including
R3605 T5934 T5933 cc and,PPARγ
R3606 T5935 T5933 conj KLF5,PPARγ
R3607 T5936 T5926 auxpass was,impaired
R3608 T5937 T5926 prep in,impaired
R3609 T5938 T5939 nmod Sam68,MEFs
R3610 T5939 T5937 pobj MEFs,in
R3611 T5940 T5938 punct −,Sam68
R3612 T5941 T5938 punct /,Sam68
R3613 T5942 T5938 punct −,Sam68
R3614 T5943 T5939 amod differentiated,MEFs
R3615 T5944 T5926 prep compared,impaired
R3616 T5945 T5944 prep with,compared
R3617 T5946 T5947 nmod Sam68,MEFs
R3618 T5947 T5945 pobj MEFs,with
R3619 T5948 T5946 punct +,Sam68
R3620 T5949 T5946 punct /,Sam68
R3621 T5950 T5946 punct +,Sam68
R3622 T5951 T5926 punct ", ",impaired
R3623 T5952 T5926 advcl consistent,impaired
R3624 T5953 T5952 prep with,consistent
R3625 T5954 T5955 amod impaired,adipogenesis
R3626 T5955 T5953 pobj adipogenesis,with
R3627 T5956 T5955 prep in,adipogenesis
R3628 T5957 T5958 det the,absence
R3629 T5958 T5956 pobj absence,in
R3630 T5959 T5958 prep of,absence
R3631 T5960 T5959 pobj Sam68,of
R3632 T5961 T5962 punct (,Figure
R3633 T5962 T5926 parataxis Figure,impaired
R3634 T5963 T5962 nummod 7,Figure
R3635 T5964 T5962 punct ),Figure
R3636 T5965 T5926 punct .,impaired
R3637 T5967 T5968 det These,data
R3638 T5968 T5969 nsubj data,support
R3639 T5970 T5968 punct ", ",data
R3640 T5971 T5968 advmod together,data
R3641 T5972 T5971 prep with,together
R3642 T5973 T5972 pobj data,with
R3643 T5974 T5973 acl confirming,data
R3644 T5975 T5976 det a,phenotype
R3645 T5976 T5974 dobj phenotype,confirming
R3646 T5977 T5976 amod lean,phenotype
R3647 T5978 T5976 prep in,phenotype
R3648 T5979 T5980 nmod Sam68,mice
R3649 T5980 T5978 pobj mice,in
R3650 T5981 T5979 punct −,Sam68
R3651 T5982 T5979 punct /,Sam68
R3652 T5983 T5979 punct −,Sam68
R3653 T5984 T5985 punct (,N.
R3654 T5985 T5968 meta N.,data
R3655 T5986 T5985 nmod Torabi,N.
R3656 T5987 T5985 cc and,N.
R3657 T5988 T5985 conj S.,N.
R3658 T5989 T5988 nmod Richard,S.
R3659 T5990 T5988 punct ", ",S.
R3660 T5991 T5992 amod unpublished,data
R3661 T5992 T5988 conj data,S.
R3662 T5993 T5992 punct ),data
R3663 T5994 T5969 punct ", ",support
R3664 T5995 T5996 det the,hypothesis
R3665 T5996 T5969 dobj hypothesis,support
R3666 T5997 T5998 mark that,modulates
R3667 T5998 T5996 acl modulates,hypothesis
R3668 T5999 T5998 nsubj Sam68,modulates
R3669 T6000 T6001 det the,differentiation
R3670 T6001 T5998 dobj differentiation,modulates
R3671 T6002 T6001 prep of,differentiation
R3672 T6003 T6004 amod mesenchymal,cells
R3673 T6004 T6002 pobj cells,of
R3674 T6005 T5969 punct .,support
R3675 T6007 T6008 aux To,examine
R3676 T6008 T6010 advcl examine,chose
R3677 T6009 T6008 advmod further,examine
R3678 T6011 T6012 det this,phenotype
R3679 T6012 T6008 dobj phenotype,examine
R3680 T6013 T6008 prep in,examine
R3681 T6014 T6015 det a,system
R3682 T6015 T6013 pobj system,in
R3683 T6016 T6017 npadvmod cell,autonomous
R3684 T6017 T6015 amod autonomous,system
R3685 T6018 T6010 punct ", ",chose
R3686 T6019 T6010 nsubj we,chose
R3687 T6020 T6021 det the,cells
R3688 T6021 T6010 dobj cells,chose
R3689 T6022 T6021 amod embryonic,cells
R3690 T6023 T6021 amod mesenchymal,cells
R3691 T6024 T6021 amod multipotential,cells
R3692 T6025 T6021 compound progenitor,cells
R3693 T6026 T6021 appos C3H10T1,cells
R3694 T6027 T6026 punct /,C3H10T1
R3695 T6028 T6026 nummod 2,C3H10T1
R3696 T6029 T6010 punct .,chose
R3697 T6031 T6032 det The,addition
R3698 T6032 T6033 nsubj addition,induced
R3699 T6034 T6032 prep of,addition
R3700 T6035 T6034 pobj BMP,of
R3701 T6036 T6035 punct -,BMP
R3702 T6037 T6035 nummod 2,BMP
R3703 T6038 T6039 compound osteoblast,differentiation
R3704 T6039 T6033 dobj differentiation,induced
R3705 T6040 T6033 punct ", ",induced
R3706 T6041 T6042 mark as,evidenced
R3707 T6042 T6033 advcl evidenced,induced
R3708 T6043 T6042 agent by,evidenced
R3709 T6044 T6045 det an,increase
R3710 T6045 T6043 pobj increase,by
R3711 T6046 T6047 advmod approximately,400-fold
R3712 T6047 T6045 nmod 400-fold,increase
R3713 T6048 T6045 prep in,increase
R3714 T6049 T6048 pobj expression,in
R3715 T6050 T6049 prep of,expression
R3716 T6051 T6052 det the,gene
R3717 T6052 T6050 pobj gene,of
R3718 T6053 T6052 nmod osteocalcin,gene
R3719 T6054 T6053 punct (,osteocalcin
R3720 T6055 T6053 appos OCN,osteocalcin
R3721 T6056 T6052 punct ),gene
R3722 T6057 T6058 punct [,51
R3723 T6058 T6033 parataxis 51,induced
R3724 T6059 T6058 punct ],51
R3725 T6060 T6033 punct .,induced
R3726 T6062 T6063 nsubjpass Populations,selected
R3727 T6064 T6062 prep of,Populations
R3728 T6065 T6066 nmod C3H10T1,cells
R3729 T6066 T6064 pobj cells,of
R3730 T6067 T6065 punct /,C3H10T1
R3731 T6068 T6065 nummod 2,C3H10T1
R3732 T6069 T6070 advmod stably,transfected
R3733 T6070 T6062 acl transfected,Populations
R3734 T6071 T6070 prep with,transfected
R3735 T6072 T6073 preconj either,retro
R3736 T6073 T6071 pobj retro,with
R3737 T6074 T6073 compound pSuper,retro
R3738 T6075 T6073 punct -,retro
R3739 T6076 T6077 punct (,control
R3740 T6077 T6073 parataxis control,retro
R3741 T6078 T6077 punct ),control
R3742 T6079 T6073 cc and,retro
R3743 T6080 T6081 compound pSuper,retro
R3744 T6081 T6073 conj retro,retro
R3745 T6082 T6081 punct -,retro
R3746 T6083 T6081 acl harboring,retro
R3747 T6084 T6085 det a,hairpin
R3748 T6085 T6083 dobj hairpin,harboring
R3749 T6086 T6085 amod short,hairpin
R3750 T6087 T6085 prep against,hairpin
R3751 T6088 T6087 pobj Sam68,against
R3752 T6089 T6085 punct (,hairpin
R3753 T6090 T6085 appos Sam68sh,hairpin
R3754 T6091 T6063 punct ),selected
R3755 T6092 T6063 auxpass were,selected
R3756 T6093 T6063 prep with,selected
R3757 T6094 T6093 pobj puromycin,with
R3758 T6095 T6063 punct .,selected
R3759 T6097 T6098 det The,expression
R3760 T6098 T6099 nsubjpass expression,reduced
R3761 T6100 T6098 prep of,expression
R3762 T6101 T6100 pobj Sam68,of
R3763 T6102 T6099 auxpass was,reduced
R3764 T6103 T6099 prep by,reduced
R3765 T6104 T6105 advmod approximately,80
R3766 T6105 T6106 nummod 80,%
R3767 T6106 T6103 pobj %,by
R3768 T6107 T6108 mark as,evidenced
R3769 T6108 T6099 advcl evidenced,reduced
R3770 T6109 T6108 agent by,evidenced
R3771 T6110 T6111 compound immunoblot,analyses
R3772 T6111 T6109 pobj analyses,by
R3773 T6112 T6111 acl using,analyses
R3774 T6113 T6114 compound β,actin
R3775 T6114 T6112 dobj actin,using
R3776 T6115 T6114 punct -,actin
R3777 T6116 T6112 prep as,using
R3778 T6117 T6118 det a,control
R3779 T6118 T6116 pobj control,as
R3780 T6119 T6118 compound loading,control
R3781 T6120 T6121 punct (,8A
R3782 T6121 T6099 parataxis 8A,reduced
R3783 T6122 T6121 compound Figure,8A
R3784 T6123 T6121 punct ),8A
R3785 T6124 T6099 punct .,reduced
R3786 T6126 T6127 compound Osteoblast,differentiation
R3787 T6127 T6128 nsubjpass differentiation,induced
R3788 T6129 T6128 auxpass was,induced
R3789 T6130 T6128 prep in,induced
R3790 T6131 T6130 pobj cultures,in
R3791 T6132 T6131 acl expressing,cultures
R3792 T6133 T6134 compound pSuper,retro
R3793 T6134 T6132 dobj retro,expressing
R3794 T6135 T6134 punct -,retro
R3795 T6136 T6134 cc or,retro
R3796 T6137 T6138 compound pSuper,retro
R3797 T6138 T6140 compound retro,Sam68sh
R3798 T6139 T6138 punct -,retro
R3799 T6140 T6134 conj Sam68sh,retro
R3800 T6141 T6128 prep by,induced
R3801 T6142 T6141 pobj addition,by
R3802 T6143 T6142 prep of,addition
R3803 T6144 T6143 pobj BMP,of
R3804 T6145 T6144 punct -,BMP
R3805 T6146 T6144 nummod 2,BMP
R3806 T6147 T6142 prep to,addition
R3807 T6148 T6149 det the,medium
R3808 T6149 T6147 pobj medium,to
R3809 T6150 T6149 compound culture,medium
R3810 T6151 T6128 punct .,induced
R3811 T6152 T6128 punct .,induced
R3812 T6154 T6155 det The,expression
R3813 T6155 T6156 nsubjpass expression,examined
R3814 T6157 T6155 prep of,expression
R3815 T6158 T6157 pobj OCN,of
R3816 T6159 T6158 cc and,OCN
R3817 T6160 T6161 compound β,actin
R3818 T6161 T6163 compound actin,mRNAs
R3819 T6162 T6161 punct -,actin
R3820 T6163 T6158 conj mRNAs,OCN
R3821 T6164 T6156 auxpass was,examined
R3822 T6165 T6156 prep by,examined
R3823 T6166 T6167 amod semiquantitative,PCR
R3824 T6167 T6165 pobj PCR,by
R3825 T6168 T6167 compound RT,PCR
R3826 T6169 T6167 punct -,PCR
R3827 T6170 T6156 cc and,examined
R3828 T6171 T6172 det the,cells
R3829 T6172 T6176 nsubj cells,displayed
R3830 T6173 T6174 npadvmod Sam68sh,expressing
R3831 T6174 T6172 amod expressing,cells
R3832 T6175 T6174 punct -,expressing
R3833 T6176 T6156 conj displayed,examined
R3834 T6177 T6178 det a,phenotype
R3835 T6178 T6176 dobj phenotype,displayed
R3836 T6179 T6180 advmod more,pronounced
R3837 T6180 T6178 amod pronounced,phenotype
R3838 T6181 T6178 compound osteoblast,phenotype
R3839 T6182 T6178 prep compared,phenotype
R3840 T6183 T6182 prep with,compared
R3841 T6184 T6185 compound control,cells
R3842 T6185 T6183 pobj cells,with
R3843 T6186 T6176 punct ", ",displayed
R3844 T6187 T6188 mark as,assessed
R3845 T6188 T6176 advcl assessed,displayed
R3846 T6189 T6188 prep by,assessed
R3847 T6190 T6191 det the,expression
R3848 T6191 T6189 pobj expression,by
R3849 T6192 T6191 prep of,expression
R3850 T6193 T6192 pobj OCN,of
R3851 T6194 T6195 punct (,8B
R3852 T6195 T6176 parataxis 8B,displayed
R3853 T6196 T6195 compound Figure,8B
R3854 T6197 T6195 punct ),8B
R3855 T6198 T6176 punct .,displayed
R3856 T6200 T6201 prep Given,stained
R3857 T6202 T6203 det the,enhancement
R3858 T6203 T6200 pobj enhancement,Given
R3859 T6204 T6203 amod apparent,enhancement
R3860 T6205 T6203 prep of,enhancement
R3861 T6206 T6207 amod mineralized,nodule
R3862 T6207 T6208 compound nodule,formation
R3863 T6208 T6205 pobj formation,of
R3864 T6209 T6203 prep by,enhancement
R3865 T6210 T6211 nmod Sam68,cells
R3866 T6211 T6209 pobj cells,by
R3867 T6212 T6210 punct −,Sam68
R3868 T6213 T6210 punct /,Sam68
R3869 T6214 T6210 punct −,Sam68
R3870 T6215 T6216 nmod bone,marrow
R3871 T6216 T6211 nmod marrow,cells
R3872 T6217 T6211 amod stromal,cells
R3873 T6218 T6219 advmod ex,vivo
R3874 T6219 T6203 advmod vivo,enhancement
R3875 T6220 T6203 cc and,enhancement
R3876 T6221 T6222 det the,phenotype
R3877 T6222 T6203 conj phenotype,enhancement
R3878 T6223 T6222 acl observed,phenotype
R3879 T6224 T6223 prep with,observed
R3880 T6225 T6226 amod short,RNA
R3881 T6226 T6228 npadvmod RNA,treated
R3882 T6227 T6226 compound hairpin,RNA
R3883 T6228 T6233 amod treated,C3H10T1
R3884 T6229 T6226 punct (,RNA
R3885 T6230 T6226 appos shRNA,RNA
R3886 T6231 T6228 punct ),treated
R3887 T6232 T6228 punct -,treated
R3888 T6233 T6224 pobj C3H10T1,with
R3889 T6234 T6233 punct /,C3H10T1
R3890 T6235 T6233 nummod 2,C3H10T1
R3891 T6236 T6201 punct ", ",stained
R3892 T6237 T6201 nsubj we,stained
R3893 T6238 T6201 dobj sections,stained
R3894 T6239 T6238 prep of,sections
R3895 T6240 T6239 pobj bone,of
R3896 T6241 T6238 prep from,sections
R3897 T6242 T6243 nummod 4,month
R3898 T6243 T6248 npadvmod month,old
R3899 T6244 T6242 punct -,4
R3900 T6245 T6242 cc and,4
R3901 T6246 T6242 conj 12,4
R3902 T6247 T6243 punct -,month
R3903 T6248 T6250 amod old,mice
R3904 T6249 T6248 punct -,old
R3905 T6250 T6241 pobj mice,from
R3906 T6251 T6201 prep for,stained
R3907 T6252 T6251 pobj evidence,for
R3908 T6253 T6252 prep of,evidence
R3909 T6254 T6253 pobj changes,of
R3910 T6255 T6254 prep in,changes
R3911 T6256 T6257 compound marrow,adiposity
R3912 T6257 T6255 pobj adiposity,in
R3913 T6258 T6201 punct .,stained
R3914 T6260 T6261 nsubj Figure,shows
R3915 T6262 T6260 nummod 9,Figure
R3916 T6263 T6261 dobj sections,shows
R3917 T6264 T6263 prep of,sections
R3918 T6265 T6266 amod undecalcified,bone
R3919 T6266 T6264 pobj bone,of
R3920 T6267 T6266 prep from,bone
R3921 T6268 T6269 nummod 4,month
R3922 T6269 T6274 npadvmod month,old
R3923 T6270 T6268 punct -,4
R3924 T6271 T6268 cc and,4
R3925 T6272 T6268 conj 12,4
R3926 T6273 T6269 punct -,month
R3927 T6274 T6276 amod old,mice
R3928 T6275 T6274 punct -,old
R3929 T6276 T6267 pobj mice,from
R3930 T6277 T6276 nmod Sam68,mice
R3931 T6278 T6277 punct +,Sam68
R3932 T6279 T6277 punct /,Sam68
R3933 T6280 T6277 punct +,Sam68
R3934 T6281 T6277 cc and,Sam68
R3935 T6282 T6277 conj Sam68,Sam68
R3936 T6283 T6282 punct −,Sam68
R3937 T6284 T6282 punct /,Sam68
R3938 T6285 T6282 punct −,Sam68
R3939 T6286 T6276 acl stained,mice
R3940 T6287 T6286 prep with,stained
R3941 T6288 T6289 compound von,Kossa
R3942 T6289 T6287 pobj Kossa,with
R3943 T6290 T6289 cc and,Kossa
R3944 T6291 T6289 conj toluidine,Kossa
R3945 T6292 T6291 amod blue,toluidine
R3946 T6293 T6294 aux to,show
R3947 T6294 T6286 advcl show,stained
R3948 T6295 T6296 amod mineralized,tissue
R3949 T6296 T6294 dobj tissue,show
R3950 T6297 T6298 punct (,9A
R3951 T6298 T6296 parataxis 9A,tissue
R3952 T6299 T6298 compound Figure,9A
R3953 T6300 T6298 punct ", ",9A
R3954 T6301 T6298 amod black,9A
R3955 T6302 T6298 punct ),9A
R3956 T6303 T6296 cc and,tissue
R3957 T6304 T6305 compound marrow,adipocytes
R3958 T6305 T6296 conj adipocytes,tissue
R3959 T6306 T6307 punct (,9B
R3960 T6307 T6305 parataxis 9B,adipocytes
R3961 T6308 T6307 compound Figure,9B
R3962 T6309 T6307 punct ", ",9B
R3963 T6310 T6307 amod white,9B
R3964 T6311 T6307 punct ),9B
R3965 T6312 T6286 cc and,stained
R3966 T6313 T6286 conj left,stained
R3967 T6314 T6313 oprd unstained,left
R3968 T6315 T6316 aux to,show
R3969 T6316 T6313 advcl show,left
R3970 T6317 T6318 compound fluorochrome,labeling
R3971 T6318 T6316 dobj labeling,show
R3972 T6319 T6318 prep of,labeling
R3973 T6320 T6321 det the,fronts
R3974 T6321 T6319 pobj fronts,of
R3975 T6322 T6321 compound mineralization,fronts
R3976 T6323 T6324 punct (,9C
R3977 T6324 T6316 parataxis 9C,show
R3978 T6325 T6324 compound Figure,9C
R3979 T6326 T6324 punct ),9C
R3980 T6327 T6261 punct .,shows
R3981 T6329 T6330 nummod Twelve,month
R3982 T6330 T6332 npadvmod month,old
R3983 T6331 T6330 punct -,month
R3984 T6332 T6334 amod old,mice
R3985 T6333 T6332 punct -,old
R3986 T6334 T6339 nsubj mice,showed
R3987 T6335 T6334 nmod Sam68,mice
R3988 T6336 T6335 punct +,Sam68
R3989 T6337 T6335 punct /,Sam68
R3990 T6338 T6335 punct +,Sam68
R3991 T6340 T6341 det a,decrease
R3992 T6341 T6339 dobj decrease,showed
R3993 T6342 T6341 amod noticeable,decrease
R3994 T6343 T6341 prep in,decrease
R3995 T6344 T6345 amod trabecular,bone
R3996 T6345 T6343 pobj bone,in
R3997 T6346 T6347 punct (,9A
R3998 T6347 T6345 parataxis 9A,bone
R3999 T6348 T6347 compound Figure,9A
R4000 T6349 T6347 punct ),9A
R4001 T6350 T6341 punct ", ",decrease
R4002 T6351 T6352 dep which,associated
R4003 T6352 T6341 relcl associated,decrease
R4004 T6353 T6352 auxpass was,associated
R4005 T6354 T6352 prep with,associated
R4006 T6355 T6356 det a,increase
R4007 T6356 T6354 pobj increase,with
R4008 T6357 T6356 amod significant,increase
R4009 T6358 T6356 prep in,increase
R4010 T6359 T6360 compound marrow,adiposity
R4011 T6360 T6358 pobj adiposity,in
R4012 T6361 T6362 punct (,9B
R4013 T6362 T6356 parataxis 9B,increase
R4014 T6363 T6362 compound Figure,9B
R4015 T6364 T6362 punct ),9B
R4016 T6365 T6354 cc and,with
R4017 T6366 T6354 conj with,with
R4018 T6367 T6368 det the,labels
R4019 T6368 T6366 pobj labels,with
R4020 T6369 T6368 nummod two,labels
R4021 T6370 T6368 compound fluorochrome,labels
R4022 T6371 T6368 acl superimposed,labels
R4023 T6372 T6371 prep upon,superimposed
R4024 T6373 T6372 pobj one,upon
R4025 T6374 T6373 det another,one
R4026 T6375 T6376 punct (,9C
R4027 T6376 T6368 parataxis 9C,labels
R4028 T6377 T6376 compound Figure,9C
R4029 T6378 T6376 punct ),9C
R4030 T6379 T6339 punct .,showed
R4031 T6381 T6382 prep In,appeared
R4032 T6383 T6381 pobj contrast,In
R4033 T6384 T6382 punct ", ",appeared
R4034 T6385 T6386 det the,bones
R4035 T6386 T6382 nsubj bones,appeared
R4036 T6387 T6386 prep of,bones
R4037 T6388 T6389 nummod 12,month
R4038 T6389 T6391 npadvmod month,old
R4039 T6390 T6389 punct -,month
R4040 T6391 T6393 amod old,mice
R4041 T6392 T6391 punct -,old
R4042 T6393 T6387 pobj mice,of
R4043 T6394 T6393 nmod Sam68,mice
R4044 T6395 T6394 punct −,Sam68
R4045 T6396 T6394 punct /,Sam68
R4046 T6397 T6394 punct −,Sam68
R4047 T6398 T6382 oprd similar,appeared
R4048 T6399 T6398 prep to,similar
R4049 T6400 T6399 pobj those,to
R4050 T6401 T6400 prep of,those
R4051 T6402 T6403 det the,mice
R4052 T6403 T6401 pobj mice,of
R4053 T6404 T6405 nummod 4,month
R4054 T6405 T6407 npadvmod month,old
R4055 T6406 T6405 punct -,month
R4056 T6407 T6403 amod old,mice
R4057 T6408 T6407 punct -,old
R4058 T6409 T6403 prep of,mice
R4059 T6410 T6411 det either,genotype
R4060 T6411 T6409 pobj genotype,of
R4061 T6412 T6382 punct .,appeared
R4062 T6414 T6415 det These,data
R4063 T6415 T6416 nsubj data,demonstrate
R4064 T6417 T6418 mark that,regulates
R4065 T6418 T6416 ccomp regulates,demonstrate
R4066 T6419 T6418 nsubj Sam68,regulates
R4067 T6420 T6421 det the,differentiation
R4068 T6421 T6418 dobj differentiation,regulates
R4069 T6422 T6421 prep of,differentiation
R4070 T6423 T6424 nmod bone,marrow
R4071 T6424 T6425 nmod marrow,cells
R4072 T6425 T6422 pobj cells,of
R4073 T6426 T6425 amod mesenchymal,cells
R4074 T6427 T6428 aux to,promote
R4075 T6428 T6418 advcl promote,regulates
R4076 T6429 T6430 compound adipocyte,differentiation
R4077 T6430 T6428 dobj differentiation,promote
R4078 T6431 T6428 cc and,promote
R4079 T6432 T6428 conj inhibit,promote
R4080 T6433 T6434 compound osteoblast,differentiation
R4081 T6434 T6432 dobj differentiation,inhibit
R4082 T6435 T6428 prep in,promote
R4083 T6436 T6437 amod aging,bone
R4084 T6437 T6435 pobj bone,in
R4085 T6438 T6416 punct .,demonstrate