PMC:1315279 JSON TXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T236	0-8	NN	denotes	Ablation
T238	0-82	sentence	denotes	Ablation of the Sam68 RNA Binding Protein Protects Mice from Age-Related Bone Loss
T239	9-11	IN	denotes	of
T240	12-15	DT	denotes	the
T242	16-21	NN	denotes	Sam68
T243	22-25	NN	denotes	RNA
T244	26-33	NN	denotes	Binding
T241	34-41	NN	denotes	Protein
T237	42-50	VBZ	denotes	Protects
T245	51-55	NNS	denotes	Mice
T246	56-60	IN	denotes	from
T247	61-64	NN	denotes	Age
T249	64-65	HYPH	denotes	-
T248	65-72	VBN	denotes	Related
T251	73-77	NN	denotes	Bone
T250	78-82	NN	denotes	Loss
T252	82-83	sentence	denotes
T254	136-139	DT	denotes	The
T253	136-345	sentence	denotes	The Src substrate associated in mitosis of 68 kDa (Sam68) is a KH-type RNA binding protein that has been shown to regulate several aspects of RNA metabolism; however, its physiologic role has remained elusive.
T256	140-143	NN	denotes	Src
T255	144-153	NN	denotes	substrate
T258	154-164	VBN	denotes	associated
T259	165-167	IN	denotes	in
T260	168-175	NN	denotes	mitosis
T261	176-178	IN	denotes	of
T262	179-181	CD	denotes	68
T263	182-185	NN	denotes	kDa
T264	186-187	-LRB-	denotes	(
T265	187-192	NN	denotes	Sam68
T266	192-193	-RRB-	denotes	)
T257	194-196	VBZ	denotes	is
T268	197-198	DT	denotes	a
T270	199-201	NN	denotes	KH
T272	201-202	HYPH	denotes	-
T271	202-206	NN	denotes	type
T273	207-210	NN	denotes	RNA
T274	211-218	NN	denotes	binding
T269	219-226	NN	denotes	protein
T275	227-231	WDT	denotes	that
T277	232-235	VBZ	denotes	has
T278	236-240	VBN	denotes	been
T276	241-246	VBN	denotes	shown
T279	247-249	TO	denotes	to
T280	250-258	VB	denotes	regulate
T281	259-266	JJ	denotes	several
T282	267-274	NNS	denotes	aspects
T283	275-277	IN	denotes	of
T284	278-281	NN	denotes	RNA
T285	282-292	NN	denotes	metabolism
T286	292-293	:	denotes	;
T287	294-301	RB	denotes	however
T288	301-303	,	denotes	,
T289	303-306	PRP$	denotes	its
T291	307-318	JJ	denotes	physiologic
T290	319-323	NN	denotes	role
T292	324-327	VBZ	denotes	has
T267	328-336	VBN	denotes	remained
T293	337-344	JJ	denotes	elusive
T294	344-345	.	denotes	.
T295	345-425	sentence	denotes	Herein we report the generation of Sam68-null mice by homologous recombination.
T296	346-352	RB	denotes	Herein
T298	353-355	PRP	denotes	we
T297	356-362	VBP	denotes	report
T299	363-366	DT	denotes	the
T300	367-377	NN	denotes	generation
T301	378-380	IN	denotes	of
T302	381-386	NN	denotes	Sam68
T304	386-387	HYPH	denotes	-
T303	387-391	JJ	denotes	null
T305	392-396	NNS	denotes	mice
T306	397-399	IN	denotes	by
T307	400-410	JJ	denotes	homologous
T308	411-424	NN	denotes	recombination
T309	424-425	.	denotes	.
T310	425-587	sentence	denotes	Aged Sam68−/− mice preserved their bone mass, in sharp contrast with 12-month-old wild-type littermates in which bone mass was decreased up to approximately 75%.
T311	426-430	VBN	denotes	Aged
T313	431-436	NN	denotes	Sam68
T314	436-437	SYM	denotes	−
T315	437-438	HYPH	denotes	/
T316	438-439	SYM	denotes	−
T312	440-444	NNS	denotes	mice
T317	445-454	VBD	denotes	preserved
T318	455-460	PRP$	denotes	their
T320	461-465	NN	denotes	bone
T319	466-470	NN	denotes	mass
T321	470-472	,	denotes	,
T322	472-474	IN	denotes	in
T323	475-480	JJ	denotes	sharp
T324	481-489	NN	denotes	contrast
T325	490-494	IN	denotes	with
T326	495-497	CD	denotes	12
T328	497-498	HYPH	denotes	-
T327	498-503	NN	denotes	month
T330	503-504	HYPH	denotes	-
T329	504-507	JJ	denotes	old
T332	508-512	JJ	denotes	wild
T334	512-513	HYPH	denotes	-
T333	513-517	NN	denotes	type
T331	518-529	NNS	denotes	littermates
T335	530-532	IN	denotes	in
T337	533-538	WDT	denotes	which
T338	539-543	NN	denotes	bone
T339	544-548	NN	denotes	mass
T340	549-552	VBD	denotes	was
T336	553-562	VBN	denotes	decreased
T341	563-565	IN	denotes	up
T343	566-568	IN	denotes	to
T344	569-582	RB	denotes	approximately
T342	583-585	CD	denotes	75
T345	585-586	NN	denotes	%
T346	586-587	.	denotes	.
T347	587-731	sentence	denotes	In fact, the bone volume of the 12-month-old Sam68−/− mice was virtually indistinguishable from that of 4-month-old wild-type or Sam68−/− mice.
T348	588-590	IN	denotes	In
T350	591-595	NN	denotes	fact
T351	595-597	,	denotes	,
T352	597-600	DT	denotes	the
T354	601-605	NN	denotes	bone
T353	606-612	NN	denotes	volume
T355	613-615	IN	denotes	of
T356	616-619	DT	denotes	the
T358	620-622	CD	denotes	12
T360	622-623	HYPH	denotes	-
T359	623-628	NN	denotes	month
T362	628-629	HYPH	denotes	-
T361	629-632	JJ	denotes	old
T363	633-638	NN	denotes	Sam68
T364	638-639	SYM	denotes	−
T365	639-640	HYPH	denotes	/
T366	640-641	SYM	denotes	−
T357	642-646	NNS	denotes	mice
T349	647-650	VBD	denotes	was
T367	651-660	RB	denotes	virtually
T368	661-678	JJ	denotes	indistinguishable
T369	679-683	IN	denotes	from
T370	684-688	DT	denotes	that
T371	689-691	IN	denotes	of
T372	692-693	CD	denotes	4
T374	693-694	HYPH	denotes	-
T373	694-699	NN	denotes	month
T376	699-700	HYPH	denotes	-
T375	700-703	JJ	denotes	old
T378	704-708	JJ	denotes	wild
T380	708-709	HYPH	denotes	-
T379	709-713	NN	denotes	type
T381	714-716	CC	denotes	or
T382	717-722	NN	denotes	Sam68
T383	722-723	SYM	denotes	−
T384	723-724	HYPH	denotes	/
T385	724-725	SYM	denotes	−
T377	726-730	NNS	denotes	mice
T386	730-731	.	denotes	.
T387	731-826	sentence	denotes	Sam68−/− bone marrow stromal cells had a differentiation advantage for the osteogenic pathway.
T388	732-737	NN	denotes	Sam68
T390	737-738	SYM	denotes	−
T391	738-739	HYPH	denotes	/
T392	739-740	SYM	denotes	−
T393	741-745	NN	denotes	bone
T394	746-752	NN	denotes	marrow
T395	753-760	JJ	denotes	stromal
T389	761-766	NNS	denotes	cells
T396	767-770	VBD	denotes	had
T397	771-772	DT	denotes	a
T399	773-788	NN	denotes	differentiation
T398	789-798	NN	denotes	advantage
T400	799-802	IN	denotes	for
T401	803-806	DT	denotes	the
T403	807-817	JJ	denotes	osteogenic
T402	818-825	NN	denotes	pathway
T404	825-826	.	denotes	.
T405	826-1105	sentence	denotes	Moreover, the knockdown of Sam68 using short hairpin RNA in the embryonic mesenchymal multipotential progenitor C3H10T1/2 cells resulted in more pronounced expression of the mature osteoblast marker osteocalcin when differentiation was induced with bone morphogenetic protein-2.
T406	827-835	RB	denotes	Moreover
T408	835-837	,	denotes	,
T409	837-840	DT	denotes	the
T410	841-850	NN	denotes	knockdown
T411	851-853	IN	denotes	of
T412	854-859	NN	denotes	Sam68
T413	860-865	VBG	denotes	using
T414	866-871	JJ	denotes	short
T416	872-879	NN	denotes	hairpin
T415	880-883	NN	denotes	RNA
T417	884-886	IN	denotes	in
T418	887-890	DT	denotes	the
T420	891-900	JJ	denotes	embryonic
T421	901-912	JJ	denotes	mesenchymal
T422	913-927	JJ	denotes	multipotential
T423	928-938	NN	denotes	progenitor
T424	939-946	NN	denotes	C3H10T1
T425	946-947	HYPH	denotes	/
T426	947-948	CD	denotes	2
T419	949-954	NNS	denotes	cells
T407	955-963	VBD	denotes	resulted
T427	964-966	IN	denotes	in
T428	967-971	RBR	denotes	more
T429	972-982	VBN	denotes	pronounced
T430	983-993	NN	denotes	expression
T431	994-996	IN	denotes	of
T432	997-1000	DT	denotes	the
T434	1001-1007	JJ	denotes	mature
T435	1008-1018	NN	denotes	osteoblast
T433	1019-1025	NN	denotes	marker
T436	1026-1037	NN	denotes	osteocalcin
T437	1038-1042	WRB	denotes	when
T439	1043-1058	NN	denotes	differentiation
T440	1059-1062	VBD	denotes	was
T438	1063-1070	VBN	denotes	induced
T441	1071-1075	IN	denotes	with
T442	1076-1080	NN	denotes	bone
T444	1081-1094	JJ	denotes	morphogenetic
T443	1095-1102	NN	denotes	protein
T445	1102-1103	HYPH	denotes	-
T446	1103-1104	CD	denotes	2
T447	1104-1105	.	denotes	.
T448	1105-1373	sentence	denotes	Cultures of mouse embryo fibroblasts generated from Sam68+/+ and Sam68−/− littermates were induced to differentiate into adipocytes with culture medium containing pioglitazone and the Sam68−/− mouse embryo fibroblasts shown to have impaired adipocyte differentiation.
T449	1106-1114	NNS	denotes	Cultures
T451	1115-1117	IN	denotes	of
T452	1118-1123	NN	denotes	mouse
T453	1124-1130	NN	denotes	embryo
T454	1131-1142	NNS	denotes	fibroblasts
T455	1143-1152	VBN	denotes	generated
T456	1153-1157	IN	denotes	from
T457	1158-1163	NN	denotes	Sam68
T459	1163-1164	SYM	denotes	+
T460	1164-1165	HYPH	denotes	/
T461	1165-1166	SYM	denotes	+
T462	1167-1170	CC	denotes	and
T463	1171-1176	NN	denotes	Sam68
T464	1176-1177	SYM	denotes	−
T465	1177-1178	HYPH	denotes	/
T466	1178-1179	SYM	denotes	−
T458	1180-1191	NNS	denotes	littermates
T467	1192-1196	VBD	denotes	were
T450	1197-1204	VBN	denotes	induced
T468	1205-1207	TO	denotes	to
T469	1208-1221	VB	denotes	differentiate
T470	1222-1226	IN	denotes	into
T471	1227-1237	NNS	denotes	adipocytes
T472	1238-1242	IN	denotes	with
T473	1243-1250	NN	denotes	culture
T474	1251-1257	NN	denotes	medium
T475	1258-1268	VBG	denotes	containing
T476	1269-1281	NN	denotes	pioglitazone
T477	1282-1285	CC	denotes	and
T478	1286-1289	DT	denotes	the
T480	1290-1295	NN	denotes	Sam68
T481	1295-1296	SYM	denotes	−
T482	1296-1297	HYPH	denotes	/
T483	1297-1298	SYM	denotes	−
T484	1299-1304	NN	denotes	mouse
T485	1305-1311	NN	denotes	embryo
T479	1312-1323	NNS	denotes	fibroblasts
T486	1324-1329	VBN	denotes	shown
T487	1330-1332	TO	denotes	to
T488	1333-1337	VB	denotes	have
T489	1338-1346	VBN	denotes	impaired
T491	1347-1356	NN	denotes	adipocyte
T490	1357-1372	NN	denotes	differentiation
T492	1372-1373	.	denotes	.
T493	1373-1585	sentence	denotes	Furthermore, in vivo it was shown that sections of bone from 12-month-old Sam68−/− mice had few marrow adipocytes compared with their age-matched wild-type littermate controls, which exhibited fatty bone marrow.
T494	1374-1385	RB	denotes	Furthermore
T496	1385-1387	,	denotes	,
T497	1387-1389	FW	denotes	in
T498	1390-1394	FW	denotes	vivo
T499	1395-1397	PRP	denotes	it
T500	1398-1401	VBD	denotes	was
T495	1402-1407	VBN	denotes	shown
T501	1408-1412	IN	denotes	that
T503	1413-1421	NNS	denotes	sections
T504	1422-1424	IN	denotes	of
T505	1425-1429	NN	denotes	bone
T506	1430-1434	IN	denotes	from
T507	1435-1437	CD	denotes	12
T509	1437-1438	HYPH	denotes	-
T508	1438-1443	NN	denotes	month
T511	1443-1444	HYPH	denotes	-
T510	1444-1447	JJ	denotes	old
T513	1448-1453	NN	denotes	Sam68
T514	1453-1454	SYM	denotes	−
T515	1454-1455	HYPH	denotes	/
T516	1455-1456	SYM	denotes	−
T512	1457-1461	NNS	denotes	mice
T502	1462-1465	VBD	denotes	had
T517	1466-1469	JJ	denotes	few
T519	1470-1476	NN	denotes	marrow
T518	1477-1487	NNS	denotes	adipocytes
T520	1488-1496	VBN	denotes	compared
T521	1497-1501	IN	denotes	with
T522	1502-1507	PRP$	denotes	their
T524	1508-1511	NN	denotes	age
T526	1511-1512	HYPH	denotes	-
T525	1512-1519	VBN	denotes	matched
T527	1520-1524	JJ	denotes	wild
T529	1524-1525	HYPH	denotes	-
T528	1525-1529	NN	denotes	type
T530	1530-1540	NN	denotes	littermate
T523	1541-1549	NNS	denotes	controls
T531	1549-1551	,	denotes	,
T532	1551-1556	WDT	denotes	which
T533	1557-1566	VBD	denotes	exhibited
T534	1567-1572	JJ	denotes	fatty
T536	1573-1577	NN	denotes	bone
T535	1578-1584	NN	denotes	marrow
T537	1584-1585	.	denotes	.
T538	1585-1872	sentence	denotes	Our findings identify endogenous Sam68 as a positive regulator of adipocyte differentiation and a negative regulator of osteoblast differentiation, which is consistent with Sam68 being a modulator of bone marrow mesenchymal cell differentiation, and hence bone metabolism, in aged mice.
T539	1586-1589	PRP$	denotes	Our
T540	1590-1598	NNS	denotes	findings
T541	1599-1607	VBP	denotes	identify
T542	1608-1618	JJ	denotes	endogenous
T543	1619-1624	NN	denotes	Sam68
T544	1625-1627	IN	denotes	as
T545	1628-1629	DT	denotes	a
T547	1630-1638	JJ	denotes	positive
T546	1639-1648	NN	denotes	regulator
T548	1649-1651	IN	denotes	of
T549	1652-1661	NN	denotes	adipocyte
T550	1662-1677	NN	denotes	differentiation
T551	1678-1681	CC	denotes	and
T552	1682-1683	DT	denotes	a
T554	1684-1692	JJ	denotes	negative
T553	1693-1702	NN	denotes	regulator
T555	1703-1705	IN	denotes	of
T556	1706-1716	NN	denotes	osteoblast
T557	1717-1732	NN	denotes	differentiation
T558	1732-1734	,	denotes	,
T559	1734-1739	WDT	denotes	which
T560	1740-1742	VBZ	denotes	is
T561	1743-1753	JJ	denotes	consistent
T562	1754-1758	IN	denotes	with
T563	1759-1764	NN	denotes	Sam68
T564	1765-1770	VBG	denotes	being
T565	1771-1772	DT	denotes	a
T566	1773-1782	NN	denotes	modulator
T567	1783-1785	IN	denotes	of
T568	1786-1790	NN	denotes	bone
T569	1791-1797	NN	denotes	marrow
T571	1798-1809	JJ	denotes	mesenchymal
T572	1810-1814	NN	denotes	cell
T570	1815-1830	NN	denotes	differentiation
T573	1830-1832	,	denotes	,
T574	1832-1835	CC	denotes	and
T575	1836-1841	RB	denotes	hence
T577	1842-1846	NN	denotes	bone
T576	1847-1857	NN	denotes	metabolism
T578	1857-1859	,	denotes	,
T579	1859-1861	IN	denotes	in
T580	1862-1866	JJ	denotes	aged
T581	1867-1871	NNS	denotes	mice
T582	1871-1872	.	denotes	.
T1057	3056-3062	IN	denotes	During
T1059	3063-3071	JJ	denotes	skeletal
T1060	3072-3083	NN	denotes	development
T1061	3083-3085	,	denotes	,
T1062	3085-3088	DT	denotes	the
T1064	3089-3097	JJ	denotes	anabolic
T1063	3098-3106	NN	denotes	activity
T1065	3107-3109	IN	denotes	of
T1066	3110-3121	NNS	denotes	osteoblasts
T1067	3122-3123	-LRB-	denotes	[
T1068	3123-3124	CD	denotes	1
T1069	3124-3125	-RRB-	denotes	]
T1070	3126-3128	VBZ	denotes	is
T1058	3129-3136	VBN	denotes	favored
T1071	3137-3141	IN	denotes	over
T1072	3142-3145	DT	denotes	the
T1074	3146-3155	JJ	denotes	catabolic
T1073	3156-3164	NN	denotes	activity
T1075	3165-3167	IN	denotes	of
T1076	3168-3179	NNS	denotes	osteoclasts
T1077	3180-3181	-LRB-	denotes	[
T1078	3181-3182	CD	denotes	2
T1079	3182-3183	-RRB-	denotes	]
T1080	3183-3185	,	denotes	,
T1081	3185-3190	WDT	denotes	which
T1082	3191-3198	VBZ	denotes	results
T1083	3199-3201	IN	denotes	in
T1084	3202-3203	DT	denotes	a
T1086	3204-3207	JJ	denotes	net
T1085	3208-3212	NN	denotes	gain
T1087	3213-3215	IN	denotes	in
T1088	3216-3220	NN	denotes	bone
T1089	3221-3225	NN	denotes	mass
T1090	3225-3226	.	denotes	.
T1091	3226-3362	sentence	denotes	At skeletal maturity, bone mass is maintained through the balanced activity of osteoblasts and osteoclasts during the remodeling cycle.
T1092	3227-3229	IN	denotes	At
T1094	3230-3238	JJ	denotes	skeletal
T1095	3239-3247	NN	denotes	maturity
T1096	3247-3249	,	denotes	,
T1097	3249-3253	NN	denotes	bone
T1098	3254-3258	NN	denotes	mass
T1099	3259-3261	VBZ	denotes	is
T1093	3262-3272	VBN	denotes	maintained
T1100	3273-3280	IN	denotes	through
T1101	3281-3284	DT	denotes	the
T1103	3285-3293	VBN	denotes	balanced
T1102	3294-3302	NN	denotes	activity
T1104	3303-3305	IN	denotes	of
T1105	3306-3317	NNS	denotes	osteoblasts
T1106	3318-3321	CC	denotes	and
T1107	3322-3333	NNS	denotes	osteoclasts
T1108	3334-3340	IN	denotes	during
T1109	3341-3344	DT	denotes	the
T1111	3345-3355	NN	denotes	remodeling
T1110	3356-3361	NN	denotes	cycle
T1112	3361-3362	.	denotes	.
T1113	3362-3502	sentence	denotes	During skeletal aging, there is a shift in the balance that favors osteoclast over osteoblast activity, which results in net bone loss [3].
T1114	3363-3369	IN	denotes	During
T1116	3370-3378	JJ	denotes	skeletal
T1117	3379-3384	NN	denotes	aging
T1118	3384-3386	,	denotes	,
T1119	3386-3391	EX	denotes	there
T1115	3392-3394	VBZ	denotes	is
T1120	3395-3396	DT	denotes	a
T1121	3397-3402	NN	denotes	shift
T1122	3403-3405	IN	denotes	in
T1123	3406-3409	DT	denotes	the
T1124	3410-3417	NN	denotes	balance
T1125	3418-3422	WDT	denotes	that
T1126	3423-3429	VBZ	denotes	favors
T1127	3430-3440	NN	denotes	osteoclast
T1128	3441-3445	IN	denotes	over
T1129	3446-3456	NN	denotes	osteoblast
T1130	3457-3465	NN	denotes	activity
T1131	3465-3467	,	denotes	,
T1132	3467-3472	WDT	denotes	which
T1133	3473-3480	VBZ	denotes	results
T1134	3481-3483	IN	denotes	in
T1135	3484-3487	JJ	denotes	net
T1137	3488-3492	NN	denotes	bone
T1136	3493-3497	NN	denotes	loss
T1138	3498-3499	-LRB-	denotes	[
T1139	3499-3500	CD	denotes	3
T1140	3500-3501	-RRB-	denotes	]
T1141	3501-3502	.	denotes	.
T1142	3502-3804	sentence	denotes	The amount and rate at which bone is gained during development and lost during aging are determined in large part by genetics [4–6] but also by physical activity and by alterations in the availability and response of bone cells to circulating hormones [7–9] and locally derived growth factors [10,11].
T1143	3503-3506	DT	denotes	The
T1144	3507-3513	NN	denotes	amount
T1146	3514-3517	CC	denotes	and
T1147	3518-3522	NN	denotes	rate
T1148	3523-3525	IN	denotes	at
T1150	3526-3531	WDT	denotes	which
T1151	3532-3536	NN	denotes	bone
T1152	3537-3539	VBZ	denotes	is
T1149	3540-3546	VBN	denotes	gained
T1153	3547-3553	IN	denotes	during
T1154	3554-3565	NN	denotes	development
T1155	3566-3569	CC	denotes	and
T1156	3570-3574	VBN	denotes	lost
T1157	3575-3581	IN	denotes	during
T1158	3582-3587	NN	denotes	aging
T1159	3588-3591	VBP	denotes	are
T1145	3592-3602	VBN	denotes	determined
T1160	3603-3605	IN	denotes	in
T1161	3606-3611	JJ	denotes	large
T1162	3612-3616	NN	denotes	part
T1163	3617-3619	IN	denotes	by
T1164	3620-3628	NN	denotes	genetics
T1165	3629-3630	-LRB-	denotes	[
T1166	3630-3631	CD	denotes	4
T1167	3631-3632	SYM	denotes	–
T1168	3632-3633	CD	denotes	6
T1169	3633-3634	-RRB-	denotes	]
T1170	3635-3638	CC	denotes	but
T1171	3639-3643	RB	denotes	also
T1172	3644-3646	IN	denotes	by
T1173	3647-3655	JJ	denotes	physical
T1174	3656-3664	NN	denotes	activity
T1175	3665-3668	CC	denotes	and
T1176	3669-3671	IN	denotes	by
T1177	3672-3683	NNS	denotes	alterations
T1178	3684-3686	IN	denotes	in
T1179	3687-3690	DT	denotes	the
T1180	3691-3703	NN	denotes	availability
T1181	3704-3707	CC	denotes	and
T1182	3708-3716	NN	denotes	response
T1183	3717-3719	IN	denotes	of
T1184	3720-3724	NN	denotes	bone
T1185	3725-3730	NNS	denotes	cells
T1186	3731-3733	IN	denotes	to
T1187	3734-3745	VBG	denotes	circulating
T1188	3746-3754	NNS	denotes	hormones
T1189	3755-3756	-LRB-	denotes	[
T1190	3756-3757	CD	denotes	7
T1191	3757-3758	SYM	denotes	–
T1192	3758-3759	CD	denotes	9
T1193	3759-3760	-RRB-	denotes	]
T1194	3761-3764	CC	denotes	and
T1195	3765-3772	RB	denotes	locally
T1196	3773-3780	VBN	denotes	derived
T1198	3781-3787	NN	denotes	growth
T1197	3788-3795	NNS	denotes	factors
T1199	3796-3797	-LRB-	denotes	[
T1201	3797-3799	CD	denotes	10
T1202	3799-3800	,	denotes	,
T1200	3800-3802	CD	denotes	11
T1203	3802-3803	-RRB-	denotes	]
T1204	3803-3804	.	denotes	.
T1205	3804-3993	sentence	denotes	Whereas genetic-based studies have provided novel insights into the pathways that regulate bone development [12–14], relatively little is known about the etiology of age-related bone loss.
T1206	3805-3812	IN	denotes	Whereas
T1208	3813-3820	JJ	denotes	genetic
T1210	3820-3821	HYPH	denotes	-
T1209	3821-3826	VBN	denotes	based
T1211	3827-3834	NNS	denotes	studies
T1212	3835-3839	VBP	denotes	have
T1207	3840-3848	VBN	denotes	provided
T1214	3849-3854	JJ	denotes	novel
T1215	3855-3863	NNS	denotes	insights
T1216	3864-3868	IN	denotes	into
T1217	3869-3872	DT	denotes	the
T1218	3873-3881	NNS	denotes	pathways
T1219	3882-3886	WDT	denotes	that
T1220	3887-3895	VBP	denotes	regulate
T1221	3896-3900	NN	denotes	bone
T1222	3901-3912	NN	denotes	development
T1223	3913-3914	-LRB-	denotes	[
T1224	3914-3916	CD	denotes	12
T1225	3916-3917	SYM	denotes	–
T1226	3917-3919	CD	denotes	14
T1227	3919-3920	-RRB-	denotes	]
T1228	3920-3922	,	denotes	,
T1229	3922-3932	RB	denotes	relatively
T1230	3933-3939	JJ	denotes	little
T1231	3940-3942	VBZ	denotes	is
T1213	3943-3948	VBN	denotes	known
T1232	3949-3954	IN	denotes	about
T1233	3955-3958	DT	denotes	the
T1234	3959-3967	NN	denotes	etiology
T1235	3968-3970	IN	denotes	of
T1236	3971-3974	NN	denotes	age
T1238	3974-3975	HYPH	denotes	-
T1237	3975-3982	VBN	denotes	related
T1240	3983-3987	NN	denotes	bone
T1239	3988-3992	NN	denotes	loss
T1241	3992-3993	.	denotes	.
T1242	3993-4151	sentence	denotes	Increased bone resorption in elderly men and women is associated with a reduction in bone mass and an increase in circulating levels of bone biomarkers [15].
T1243	3994-4003	VBN	denotes	Increased
T1245	4004-4008	NN	denotes	bone
T1244	4009-4019	NN	denotes	resorption
T1247	4020-4022	IN	denotes	in
T1248	4023-4030	JJ	denotes	elderly
T1249	4031-4034	NNS	denotes	men
T1250	4035-4038	CC	denotes	and
T1251	4039-4044	NNS	denotes	women
T1252	4045-4047	VBZ	denotes	is
T1246	4048-4058	VBN	denotes	associated
T1253	4059-4063	IN	denotes	with
T1254	4064-4065	DT	denotes	a
T1255	4066-4075	NN	denotes	reduction
T1256	4076-4078	IN	denotes	in
T1257	4079-4083	NN	denotes	bone
T1258	4084-4088	NN	denotes	mass
T1259	4089-4092	CC	denotes	and
T1260	4093-4095	DT	denotes	an
T1261	4096-4104	NN	denotes	increase
T1262	4105-4107	IN	denotes	in
T1263	4108-4119	VBG	denotes	circulating
T1264	4120-4126	NNS	denotes	levels
T1265	4127-4129	IN	denotes	of
T1266	4130-4134	NN	denotes	bone
T1267	4135-4145	NNS	denotes	biomarkers
T1268	4146-4147	-LRB-	denotes	[
T1269	4147-4149	CD	denotes	15
T1270	4149-4150	-RRB-	denotes	]
T1271	4150-4151	.	denotes	.
T1272	4151-4440	sentence	denotes	These changes have been attributed primarily to nutritional deficits resulting in alterations in the parathyroid hormone–vitamin D axis [16], to gonadal hormone deficiency [17], to leptin levels and the sympathetic nervous system [8,18–20], and to alterations in bone cell apoptosis [21].
T1273	4152-4157	DT	denotes	These
T1274	4158-4165	NNS	denotes	changes
T1276	4166-4170	VBP	denotes	have
T1277	4171-4175	VBN	denotes	been
T1275	4176-4186	VBN	denotes	attributed
T1278	4187-4196	RB	denotes	primarily
T1279	4197-4199	IN	denotes	to
T1280	4200-4211	JJ	denotes	nutritional
T1281	4212-4220	NNS	denotes	deficits
T1282	4221-4230	VBG	denotes	resulting
T1283	4231-4233	IN	denotes	in
T1284	4234-4245	NNS	denotes	alterations
T1285	4246-4248	IN	denotes	in
T1286	4249-4252	DT	denotes	the
T1288	4253-4264	NN	denotes	parathyroid
T1289	4265-4272	NN	denotes	hormone
T1290	4272-4273	HYPH	denotes	–
T1291	4273-4280	NN	denotes	vitamin
T1292	4281-4282	NN	denotes	D
T1287	4283-4287	NN	denotes	axis
T1293	4288-4289	-LRB-	denotes	[
T1294	4289-4291	CD	denotes	16
T1295	4291-4292	-RRB-	denotes	]
T1296	4292-4294	,	denotes	,
T1297	4294-4296	IN	denotes	to
T1298	4297-4304	JJ	denotes	gonadal
T1300	4305-4312	NN	denotes	hormone
T1299	4313-4323	NN	denotes	deficiency
T1301	4324-4325	-LRB-	denotes	[
T1302	4325-4327	CD	denotes	17
T1303	4327-4328	-RRB-	denotes	]
T1304	4328-4330	,	denotes	,
T1305	4330-4332	IN	denotes	to
T1306	4333-4339	NN	denotes	leptin
T1307	4340-4346	NNS	denotes	levels
T1308	4347-4350	CC	denotes	and
T1309	4351-4354	DT	denotes	the
T1311	4355-4366	JJ	denotes	sympathetic
T1312	4367-4374	JJ	denotes	nervous
T1310	4375-4381	NN	denotes	system
T1313	4382-4383	-LRB-	denotes	[
T1314	4383-4384	CD	denotes	8
T1315	4384-4385	,	denotes	,
T1316	4385-4387	CD	denotes	18
T1317	4387-4388	SYM	denotes	–
T1318	4388-4390	CD	denotes	20
T1319	4390-4391	-RRB-	denotes	]
T1320	4391-4393	,	denotes	,
T1321	4393-4396	CC	denotes	and
T1322	4397-4399	IN	denotes	to
T1323	4400-4411	NNS	denotes	alterations
T1324	4412-4414	IN	denotes	in
T1325	4415-4419	NN	denotes	bone
T1326	4420-4424	NN	denotes	cell
T1327	4425-4434	NN	denotes	apoptosis
T1328	4435-4436	-LRB-	denotes	[
T1329	4436-4438	CD	denotes	21
T1330	4438-4439	-RRB-	denotes	]
T1331	4439-4440	.	denotes	.
T1332	4440-4672	sentence	denotes	Bone loss in the elderly has also been attributed to alterations in the response of bone marrow stromal cells to their microenvironment that favors differentiation down the adipocyte lineage rather than the osteoblast lineage [22].
T1333	4441-4445	NN	denotes	Bone
T1334	4446-4450	NN	denotes	loss
T1336	4451-4453	IN	denotes	in
T1337	4454-4457	DT	denotes	the
T1338	4458-4465	JJ	denotes	elderly
T1339	4466-4469	VBZ	denotes	has
T1340	4470-4474	RB	denotes	also
T1341	4475-4479	VBN	denotes	been
T1335	4480-4490	VBN	denotes	attributed
T1342	4491-4493	IN	denotes	to
T1343	4494-4505	NNS	denotes	alterations
T1344	4506-4508	IN	denotes	in
T1345	4509-4512	DT	denotes	the
T1346	4513-4521	NN	denotes	response
T1347	4522-4524	IN	denotes	of
T1348	4525-4529	NN	denotes	bone
T1349	4530-4536	NN	denotes	marrow
T1351	4537-4544	JJ	denotes	stromal
T1350	4545-4550	NNS	denotes	cells
T1352	4551-4553	IN	denotes	to
T1353	4554-4559	PRP$	denotes	their
T1354	4560-4576	NN	denotes	microenvironment
T1355	4577-4581	WDT	denotes	that
T1356	4582-4588	VBZ	denotes	favors
T1357	4589-4604	NN	denotes	differentiation
T1358	4605-4609	IN	denotes	down
T1359	4610-4613	DT	denotes	the
T1361	4614-4623	NN	denotes	adipocyte
T1360	4624-4631	NN	denotes	lineage
T1362	4632-4638	RB	denotes	rather
T1363	4639-4643	IN	denotes	than
T1364	4644-4647	DT	denotes	the
T1366	4648-4658	NN	denotes	osteoblast
T1365	4659-4666	NN	denotes	lineage
T1367	4667-4668	-LRB-	denotes	[
T1368	4668-4670	CD	denotes	22
T1369	4670-4671	-RRB-	denotes	]
T1370	4671-4672	.	denotes	.
T1371	4672-4804	sentence	denotes	Aging has long been associated with an increase in marrow fat, where the generation of adipocytes is favored over osteoblasts [23].
T1372	4673-4678	NN	denotes	Aging
T1374	4679-4682	VBZ	denotes	has
T1375	4683-4687	RB	denotes	long
T1376	4688-4692	VBN	denotes	been
T1373	4693-4703	VBN	denotes	associated
T1377	4704-4708	IN	denotes	with
T1378	4709-4711	DT	denotes	an
T1379	4712-4720	NN	denotes	increase
T1380	4721-4723	IN	denotes	in
T1381	4724-4730	NN	denotes	marrow
T1382	4731-4734	NN	denotes	fat
T1383	4734-4736	,	denotes	,
T1384	4736-4741	WRB	denotes	where
T1386	4742-4745	DT	denotes	the
T1387	4746-4756	NN	denotes	generation
T1388	4757-4759	IN	denotes	of
T1389	4760-4770	NNS	denotes	adipocytes
T1390	4771-4773	VBZ	denotes	is
T1385	4774-4781	VBN	denotes	favored
T1391	4782-4786	IN	denotes	over
T1392	4787-4798	NNS	denotes	osteoblasts
T1393	4799-4800	-LRB-	denotes	[
T1394	4800-4802	CD	denotes	23
T1395	4802-4803	-RRB-	denotes	]
T1396	4803-4804	.	denotes	.
T1397	4804-5024	sentence	denotes	Osteoblasts and adipocytes are derived from a common mesenchymal precursor cell present in bone marrow, and the factors that control this age-induced switch toward adipogenic differentiation is not well understood [24].
T1398	4805-4816	NNS	denotes	Osteoblasts
T1400	4817-4820	CC	denotes	and
T1401	4821-4831	NNS	denotes	adipocytes
T1402	4832-4835	VBP	denotes	are
T1399	4836-4843	VBN	denotes	derived
T1403	4844-4848	IN	denotes	from
T1404	4849-4850	DT	denotes	a
T1406	4851-4857	JJ	denotes	common
T1407	4858-4869	JJ	denotes	mesenchymal
T1408	4870-4879	NN	denotes	precursor
T1405	4880-4884	NN	denotes	cell
T1409	4885-4892	JJ	denotes	present
T1410	4893-4895	IN	denotes	in
T1411	4896-4900	NN	denotes	bone
T1412	4901-4907	NN	denotes	marrow
T1413	4907-4909	,	denotes	,
T1414	4909-4912	CC	denotes	and
T1415	4913-4916	DT	denotes	the
T1416	4917-4924	NNS	denotes	factors
T1418	4925-4929	WDT	denotes	that
T1419	4930-4937	VBP	denotes	control
T1420	4938-4942	DT	denotes	this
T1422	4943-4946	NN	denotes	age
T1424	4946-4947	HYPH	denotes	-
T1423	4947-4954	VBN	denotes	induced
T1421	4955-4961	NN	denotes	switch
T1425	4962-4968	IN	denotes	toward
T1426	4969-4979	JJ	denotes	adipogenic
T1427	4980-4995	NN	denotes	differentiation
T1428	4996-4998	VBZ	denotes	is
T1429	4999-5002	RB	denotes	not
T1430	5003-5007	RB	denotes	well
T1417	5008-5018	VBN	denotes	understood
T1431	5019-5020	-LRB-	denotes	[
T1432	5020-5022	CD	denotes	24
T1433	5022-5023	-RRB-	denotes	]
T1434	5023-5024	.	denotes	.
T1435	5024-5305	sentence	denotes	While several transcriptional regulatory proteins have been associated with cell fate determination of bone marrow mesenchymal cells, including peroxisome proliferator–activated receptor γ (PPARγ) and KLF5 [25–30], the role of RNA binding proteins in this process remains unknown.
T1436	5025-5030	IN	denotes	While
T1438	5031-5038	JJ	denotes	several
T1440	5039-5054	JJ	denotes	transcriptional
T1441	5055-5065	JJ	denotes	regulatory
T1439	5066-5074	NN	denotes	proteins
T1442	5075-5079	VBP	denotes	have
T1443	5080-5084	VBN	denotes	been
T1437	5085-5095	VBN	denotes	associated
T1445	5096-5100	IN	denotes	with
T1446	5101-5105	NN	denotes	cell
T1448	5106-5110	NN	denotes	fate
T1447	5111-5124	NN	denotes	determination
T1449	5125-5127	IN	denotes	of
T1450	5128-5132	NN	denotes	bone
T1451	5133-5139	NN	denotes	marrow
T1453	5140-5151	JJ	denotes	mesenchymal
T1452	5152-5157	NNS	denotes	cells
T1454	5157-5159	,	denotes	,
T1455	5159-5168	VBG	denotes	including
T1456	5169-5179	NN	denotes	peroxisome
T1458	5180-5192	NN	denotes	proliferator
T1460	5192-5193	HYPH	denotes	–
T1459	5193-5202	VBN	denotes	activated
T1457	5203-5211	NN	denotes	receptor
T1461	5212-5213	SYM	denotes	γ
T1462	5214-5215	-LRB-	denotes	(
T1463	5215-5220	NN	denotes	PPARγ
T1464	5220-5221	-RRB-	denotes	)
T1465	5222-5225	CC	denotes	and
T1466	5226-5230	NN	denotes	KLF5
T1467	5231-5232	-LRB-	denotes	[
T1468	5232-5234	CD	denotes	25
T1469	5234-5235	SYM	denotes	–
T1470	5235-5237	CD	denotes	30
T1471	5237-5238	-RRB-	denotes	]
T1472	5238-5240	,	denotes	,
T1473	5240-5243	DT	denotes	the
T1474	5244-5248	NN	denotes	role
T1475	5249-5251	IN	denotes	of
T1476	5252-5255	NN	denotes	RNA
T1478	5256-5263	NN	denotes	binding
T1477	5264-5272	NN	denotes	proteins
T1479	5273-5275	IN	denotes	in
T1480	5276-5280	DT	denotes	this
T1481	5281-5288	NN	denotes	process
T1444	5289-5296	VBZ	denotes	remains
T1482	5297-5304	JJ	denotes	unknown
T1483	5304-5305	.	denotes	.
T1484	5305-5391	sentence	denotes	RNA binding proteins of the KH type are known regulators of cellular differentiation.
T1485	5306-5309	NN	denotes	RNA
T1487	5310-5317	NN	denotes	binding
T1486	5318-5326	NN	denotes	proteins
T1489	5327-5329	IN	denotes	of
T1490	5330-5333	DT	denotes	the
T1492	5334-5336	NN	denotes	KH
T1491	5337-5341	NN	denotes	type
T1488	5342-5345	VBP	denotes	are
T1493	5346-5351	JJ	denotes	known
T1494	5352-5362	NNS	denotes	regulators
T1495	5363-5365	IN	denotes	of
T1496	5366-5374	JJ	denotes	cellular
T1497	5375-5390	NN	denotes	differentiation
T1498	5390-5391	.	denotes	.
T1499	5391-5581	sentence	denotes	For example, expression defects in the KH domain proteins NOVA and FMRP are known to cause paraneoplastic neurologic disorders [31] and the fragile X syndrome, respectively, in humans [32].
T1500	5392-5395	IN	denotes	For
T1502	5396-5403	NN	denotes	example
T1503	5403-5405	,	denotes	,
T1504	5405-5415	NN	denotes	expression
T1505	5416-5423	NNS	denotes	defects
T1506	5424-5426	IN	denotes	in
T1507	5427-5430	DT	denotes	the
T1509	5431-5433	NN	denotes	KH
T1510	5434-5440	NN	denotes	domain
T1508	5441-5449	NN	denotes	proteins
T1511	5450-5454	NN	denotes	NOVA
T1512	5455-5458	CC	denotes	and
T1513	5459-5463	NN	denotes	FMRP
T1514	5464-5467	VBP	denotes	are
T1501	5468-5473	VBN	denotes	known
T1515	5474-5476	TO	denotes	to
T1516	5477-5482	VB	denotes	cause
T1517	5483-5497	JJ	denotes	paraneoplastic
T1519	5498-5508	JJ	denotes	neurologic
T1518	5509-5518	NNS	denotes	disorders
T1520	5519-5520	-LRB-	denotes	[
T1521	5520-5522	CD	denotes	31
T1522	5522-5523	-RRB-	denotes	]
T1523	5524-5527	CC	denotes	and
T1524	5528-5531	DT	denotes	the
T1526	5532-5539	JJ	denotes	fragile
T1527	5540-5541	NN	denotes	X
T1525	5542-5550	NN	denotes	syndrome
T1528	5550-5552	,	denotes	,
T1529	5552-5564	RB	denotes	respectively
T1530	5564-5566	,	denotes	,
T1531	5566-5568	IN	denotes	in
T1532	5569-5575	NNS	denotes	humans
T1533	5576-5577	-LRB-	denotes	[
T1534	5577-5579	CD	denotes	32
T1535	5579-5580	-RRB-	denotes	]
T1536	5580-5581	.	denotes	.
T1537	5581-5716	sentence	denotes	The phenotype of the quaking viable mice suggests a role for the QUAKING RNA binding protein in oligodendrocytes and myelination [33].
T1538	5582-5585	DT	denotes	The
T1539	5586-5595	NN	denotes	phenotype
T1541	5596-5598	IN	denotes	of
T1542	5599-5602	DT	denotes	the
T1544	5603-5610	NN	denotes	quaking
T1545	5611-5617	JJ	denotes	viable
T1543	5618-5622	NNS	denotes	mice
T1540	5623-5631	VBZ	denotes	suggests
T1546	5632-5633	DT	denotes	a
T1547	5634-5638	NN	denotes	role
T1548	5639-5642	IN	denotes	for
T1549	5643-5646	DT	denotes	the
T1551	5647-5654	NN	denotes	QUAKING
T1552	5655-5658	NN	denotes	RNA
T1553	5659-5666	NN	denotes	binding
T1550	5667-5674	NN	denotes	protein
T1554	5675-5677	IN	denotes	in
T1555	5678-5694	NNS	denotes	oligodendrocytes
T1556	5695-5698	CC	denotes	and
T1557	5699-5710	NN	denotes	myelination
T1558	5711-5712	-LRB-	denotes	[
T1559	5712-5714	CD	denotes	33
T1560	5714-5715	-RRB-	denotes	]
T1561	5715-5716	.	denotes	.
T1562	5716-5915	sentence	denotes	Indeed, the ectopic expression of the QKI-6/7 isoforms in vivo led to the formation of glial cells rather than neurons from neural progenitor, demonstrating its role in cell fate determination [34].
T1563	5717-5723	RB	denotes	Indeed
T1565	5723-5725	,	denotes	,
T1566	5725-5728	DT	denotes	the
T1568	5729-5736	JJ	denotes	ectopic
T1567	5737-5747	NN	denotes	expression
T1569	5748-5750	IN	denotes	of
T1570	5751-5754	DT	denotes	the
T1572	5755-5758	NN	denotes	QKI
T1574	5758-5759	HYPH	denotes	-
T1575	5759-5760	CD	denotes	6
T1576	5760-5761	HYPH	denotes	/
T1573	5761-5762	CD	denotes	7
T1571	5763-5771	NNS	denotes	isoforms
T1577	5772-5774	FW	denotes	in
T1578	5775-5779	FW	denotes	vivo
T1564	5780-5783	VBD	denotes	led
T1579	5784-5786	IN	denotes	to
T1580	5787-5790	DT	denotes	the
T1581	5791-5800	NN	denotes	formation
T1582	5801-5803	IN	denotes	of
T1583	5804-5809	JJ	denotes	glial
T1584	5810-5815	NNS	denotes	cells
T1585	5816-5822	RB	denotes	rather
T1586	5823-5827	IN	denotes	than
T1587	5828-5835	NNS	denotes	neurons
T1588	5836-5840	IN	denotes	from
T1589	5841-5847	JJ	denotes	neural
T1590	5848-5858	NN	denotes	progenitor
T1591	5858-5860	,	denotes	,
T1592	5860-5873	VBG	denotes	demonstrating
T1593	5874-5877	PRP$	denotes	its
T1594	5878-5882	NN	denotes	role
T1595	5883-5885	IN	denotes	in
T1596	5886-5890	NN	denotes	cell
T1598	5891-5895	NN	denotes	fate
T1597	5896-5909	NN	denotes	determination
T1599	5910-5911	-LRB-	denotes	[
T1600	5911-5913	CD	denotes	34
T1601	5913-5914	-RRB-	denotes	]
T1602	5914-5915	.	denotes	.
T1603	5915-6152	sentence	denotes	The loss of GLD-1 protein in Caenorhabditis elegans prevents the appearance of stem cells [35], and the absence of the KH domain protein HOW in Drosophila prevents muscle differentiation and results in flies with held-out-wings [36,37].
T1604	5916-5919	DT	denotes	The
T1605	5920-5924	NN	denotes	loss
T1607	5925-5927	IN	denotes	of
T1608	5928-5931	NN	denotes	GLD
T1610	5931-5932	HYPH	denotes	-
T1611	5932-5933	CD	denotes	1
T1609	5934-5941	NN	denotes	protein
T1612	5942-5944	IN	denotes	in
T1613	5945-5959	NNP	denotes	Caenorhabditis
T1614	5960-5967	NNP	denotes	elegans
T1606	5968-5976	VBZ	denotes	prevents
T1615	5977-5980	DT	denotes	the
T1616	5981-5991	NN	denotes	appearance
T1617	5992-5994	IN	denotes	of
T1618	5995-5999	NN	denotes	stem
T1619	6000-6005	NNS	denotes	cells
T1620	6006-6007	-LRB-	denotes	[
T1621	6007-6009	CD	denotes	35
T1622	6009-6010	-RRB-	denotes	]
T1623	6010-6012	,	denotes	,
T1624	6012-6015	CC	denotes	and
T1625	6016-6019	DT	denotes	the
T1626	6020-6027	NN	denotes	absence
T1628	6028-6030	IN	denotes	of
T1629	6031-6034	DT	denotes	the
T1631	6035-6037	NN	denotes	KH
T1632	6038-6044	NN	denotes	domain
T1630	6045-6052	NN	denotes	protein
T1633	6053-6056	NN	denotes	HOW
T1634	6057-6059	IN	denotes	in
T1635	6060-6070	NNP	denotes	Drosophila
T1627	6071-6079	VBZ	denotes	prevents
T1636	6080-6086	NN	denotes	muscle
T1637	6087-6102	NN	denotes	differentiation
T1638	6103-6106	CC	denotes	and
T1639	6107-6114	VBZ	denotes	results
T1640	6115-6117	IN	denotes	in
T1641	6118-6123	NNS	denotes	flies
T1642	6124-6128	IN	denotes	with
T1643	6129-6133	VBN	denotes	held
T1645	6133-6134	HYPH	denotes	-
T1646	6134-6137	RP	denotes	out
T1647	6137-6138	HYPH	denotes	-
T1644	6138-6143	NNS	denotes	wings
T1648	6144-6145	-LRB-	denotes	[
T1650	6145-6147	CD	denotes	36
T1651	6147-6148	,	denotes	,
T1649	6148-6150	CD	denotes	37
T1652	6150-6151	-RRB-	denotes	]
T1653	6151-6152	.	denotes	.
T1654	6152-6335	sentence	denotes	The Src substrate associated in mitosis of 68 kDa (Sam68) is also a member of the family of KH domain RNA binding proteins [38]; however, its physiologic role has remained undefined.
T1655	6153-6156	DT	denotes	The
T1657	6157-6160	NN	denotes	Src
T1656	6161-6170	NN	denotes	substrate
T1659	6171-6181	VBN	denotes	associated
T1660	6182-6184	IN	denotes	in
T1661	6185-6192	NN	denotes	mitosis
T1662	6193-6195	IN	denotes	of
T1663	6196-6198	CD	denotes	68
T1664	6199-6202	NN	denotes	kDa
T1665	6203-6204	-LRB-	denotes	(
T1666	6204-6209	NN	denotes	Sam68
T1667	6209-6210	-RRB-	denotes	)
T1658	6211-6213	VBZ	denotes	is
T1669	6214-6218	RB	denotes	also
T1670	6219-6220	DT	denotes	a
T1671	6221-6227	NN	denotes	member
T1672	6228-6230	IN	denotes	of
T1673	6231-6234	DT	denotes	the
T1674	6235-6241	NN	denotes	family
T1675	6242-6244	IN	denotes	of
T1676	6245-6247	NN	denotes	KH
T1678	6248-6254	NN	denotes	domain
T1679	6255-6258	NN	denotes	RNA
T1680	6259-6266	NN	denotes	binding
T1677	6267-6275	NN	denotes	proteins
T1681	6276-6277	-LRB-	denotes	[
T1682	6277-6279	CD	denotes	38
T1683	6279-6280	-RRB-	denotes	]
T1684	6280-6281	:	denotes	;
T1685	6282-6289	RB	denotes	however
T1686	6289-6291	,	denotes	,
T1687	6291-6294	PRP$	denotes	its
T1689	6295-6306	JJ	denotes	physiologic
T1688	6307-6311	NN	denotes	role
T1690	6312-6315	VBZ	denotes	has
T1668	6316-6324	VBN	denotes	remained
T1691	6325-6334	JJ	denotes	undefined
T1692	6334-6335	.	denotes	.
T1693	6335-6514	sentence	denotes	Sam68 was identified as an SH3 and SH2 domain interacting protein for Src family kinases and is also a known substrate of Src kinases [39–42] and of the breast tumor kinase [43].
T1694	6336-6341	NN	denotes	Sam68
T1696	6342-6345	VBD	denotes	was
T1695	6346-6356	VBN	denotes	identified
T1697	6357-6359	IN	denotes	as
T1698	6360-6362	DT	denotes	an
T1700	6363-6366	NN	denotes	SH3
T1701	6367-6370	CC	denotes	and
T1702	6371-6374	NN	denotes	SH2
T1703	6375-6381	NN	denotes	domain
T1704	6382-6393	VBG	denotes	interacting
T1699	6394-6401	NN	denotes	protein
T1705	6402-6405	IN	denotes	for
T1706	6406-6409	NN	denotes	Src
T1708	6410-6416	NN	denotes	family
T1707	6417-6424	NNS	denotes	kinases
T1709	6425-6428	CC	denotes	and
T1710	6429-6431	VBZ	denotes	is
T1711	6432-6436	RB	denotes	also
T1712	6437-6438	DT	denotes	a
T1714	6439-6444	JJ	denotes	known
T1713	6445-6454	NN	denotes	substrate
T1715	6455-6457	IN	denotes	of
T1716	6458-6461	NN	denotes	Src
T1717	6462-6469	NNS	denotes	kinases
T1718	6470-6471	-LRB-	denotes	[
T1719	6471-6473	CD	denotes	39
T1720	6473-6474	SYM	denotes	–
T1721	6474-6476	CD	denotes	42
T1722	6476-6477	-RRB-	denotes	]
T1723	6478-6481	CC	denotes	and
T1724	6482-6484	IN	denotes	of
T1725	6485-6488	DT	denotes	the
T1727	6489-6495	NN	denotes	breast
T1728	6496-6501	NN	denotes	tumor
T1726	6502-6508	NN	denotes	kinase
T1729	6509-6510	-LRB-	denotes	[
T1730	6510-6512	CD	denotes	43
T1731	6512-6513	-RRB-	denotes	]
T1732	6513-6514	.	denotes	.
T1733	6514-6628	sentence	denotes	Sam68 has been shown to facilitate the export of unspliced HIV RNA [44] and to regulate pre-mRNA processing [45].
T1734	6515-6520	NN	denotes	Sam68
T1736	6521-6524	VBZ	denotes	has
T1737	6525-6529	VBN	denotes	been
T1735	6530-6535	VBN	denotes	shown
T1738	6536-6538	TO	denotes	to
T1739	6539-6549	VB	denotes	facilitate
T1740	6550-6553	DT	denotes	the
T1741	6554-6560	NN	denotes	export
T1742	6561-6563	IN	denotes	of
T1743	6564-6573	JJ	denotes	unspliced
T1745	6574-6577	NN	denotes	HIV
T1744	6578-6581	NN	denotes	RNA
T1746	6582-6583	-LRB-	denotes	[
T1747	6583-6585	CD	denotes	44
T1748	6585-6586	-RRB-	denotes	]
T1749	6587-6590	CC	denotes	and
T1750	6591-6593	TO	denotes	to
T1751	6594-6602	VB	denotes	regulate
T1752	6603-6611	NN	denotes	pre-mRNA
T1753	6612-6622	NN	denotes	processing
T1754	6623-6624	-LRB-	denotes	[
T1755	6624-6626	CD	denotes	45
T1756	6626-6627	-RRB-	denotes	]
T1757	6627-6628	.	denotes	.
T1758	6628-6734	sentence	denotes	In the present paper, we report the generation of Sam68−/− mice and analysis of their skeletal phenotype.
T1759	6629-6631	IN	denotes	In
T1761	6632-6635	DT	denotes	the
T1763	6636-6643	JJ	denotes	present
T1762	6644-6649	NN	denotes	paper
T1764	6649-6651	,	denotes	,
T1765	6651-6653	PRP	denotes	we
T1760	6654-6660	VBP	denotes	report
T1766	6661-6664	DT	denotes	the
T1767	6665-6675	NN	denotes	generation
T1768	6676-6678	IN	denotes	of
T1769	6679-6684	NN	denotes	Sam68
T1771	6684-6685	SYM	denotes	−
T1772	6685-6686	HYPH	denotes	/
T1773	6686-6687	SYM	denotes	−
T1770	6688-6692	NNS	denotes	mice
T1774	6693-6696	CC	denotes	and
T1775	6697-6705	NN	denotes	analysis
T1776	6706-6708	IN	denotes	of
T1777	6709-6714	PRP$	denotes	their
T1779	6715-6723	JJ	denotes	skeletal
T1778	6724-6733	NN	denotes	phenotype
T1780	6733-6734	.	denotes	.
T1781	6734-6908	sentence	denotes	Our data indicate that the absence of Sam68 confers resistance to age-related bone loss in mice such that old Sam68 have a higher bone mass than their wild-type littermates.
T1782	6735-6738	PRP$	denotes	Our
T1783	6739-6743	NNS	denotes	data
T1784	6744-6752	VBP	denotes	indicate
T1785	6753-6757	IN	denotes	that
T1787	6758-6761	DT	denotes	the
T1788	6762-6769	NN	denotes	absence
T1789	6770-6772	IN	denotes	of
T1790	6773-6778	NN	denotes	Sam68
T1786	6779-6786	VBZ	denotes	confers
T1791	6787-6797	NN	denotes	resistance
T1792	6798-6800	IN	denotes	to
T1793	6801-6804	NN	denotes	age
T1795	6804-6805	HYPH	denotes	-
T1794	6805-6812	VBN	denotes	related
T1797	6813-6817	NN	denotes	bone
T1796	6818-6822	NN	denotes	loss
T1798	6823-6825	IN	denotes	in
T1799	6826-6830	NNS	denotes	mice
T1800	6831-6835	JJ	denotes	such
T1802	6836-6840	IN	denotes	that
T1803	6841-6844	JJ	denotes	old
T1804	6845-6850	NN	denotes	Sam68
T1801	6851-6855	VBP	denotes	have
T1805	6856-6857	DT	denotes	a
T1807	6858-6864	JJR	denotes	higher
T1808	6865-6869	NN	denotes	bone
T1806	6870-6874	NN	denotes	mass
T1809	6875-6879	IN	denotes	than
T1810	6880-6885	PRP$	denotes	their
T1812	6886-6890	JJ	denotes	wild
T1814	6890-6891	HYPH	denotes	-
T1813	6891-6895	NN	denotes	type
T1811	6896-6907	NNS	denotes	littermates
T1815	6907-6908	.	denotes	.
T1816	6908-7167	sentence	denotes	We provide evidence that Sam68 regulates the differentiation of bone marrow stromal cells by showing that cells isolated from Sam68−/− animals had enhanced osteogenic activity and decreased adipogenic activity than those harvested from wild-type littermates.
T1817	6909-6911	PRP	denotes	We
T1818	6912-6919	VBP	denotes	provide
T1819	6920-6928	NN	denotes	evidence
T1820	6929-6933	IN	denotes	that
T1822	6934-6939	NN	denotes	Sam68
T1821	6940-6949	VBZ	denotes	regulates
T1823	6950-6953	DT	denotes	the
T1824	6954-6969	NN	denotes	differentiation
T1825	6970-6972	IN	denotes	of
T1826	6973-6977	NN	denotes	bone
T1827	6978-6984	NN	denotes	marrow
T1829	6985-6992	JJ	denotes	stromal
T1828	6993-6998	NNS	denotes	cells
T1830	6999-7001	IN	denotes	by
T1831	7002-7009	VBG	denotes	showing
T1832	7010-7014	IN	denotes	that
T1834	7015-7020	NNS	denotes	cells
T1835	7021-7029	VBN	denotes	isolated
T1836	7030-7034	IN	denotes	from
T1837	7035-7040	NN	denotes	Sam68
T1839	7040-7041	SYM	denotes	−
T1840	7041-7042	HYPH	denotes	/
T1841	7042-7043	SYM	denotes	−
T1838	7044-7051	NNS	denotes	animals
T1833	7052-7055	VBD	denotes	had
T1842	7056-7064	VBN	denotes	enhanced
T1844	7065-7075	JJ	denotes	osteogenic
T1843	7076-7084	NN	denotes	activity
T1845	7085-7088	CC	denotes	and
T1846	7089-7098	VBN	denotes	decreased
T1848	7099-7109	JJ	denotes	adipogenic
T1847	7110-7118	NN	denotes	activity
T1849	7119-7123	IN	denotes	than
T1850	7124-7129	DT	denotes	those
T1851	7130-7139	VBN	denotes	harvested
T1852	7140-7144	IN	denotes	from
T1853	7145-7149	JJ	denotes	wild
T1855	7149-7150	HYPH	denotes	-
T1854	7150-7154	NN	denotes	type
T1856	7155-7166	NNS	denotes	littermates
T1857	7166-7167	.	denotes	.
T1858	7167-7376	sentence	denotes	Furthermore, Sam68−/− mouse embryo fibroblasts (MEFs) were impaired in their capacity to differentiate into adipocytes, consistent with Sam68 being a regulator of bone marrow mesenchymal cell differentiation.
T1859	7168-7179	RB	denotes	Furthermore
T1861	7179-7181	,	denotes	,
T1862	7181-7186	NN	denotes	Sam68
T1863	7186-7187	SYM	denotes	−
T1864	7187-7188	HYPH	denotes	/
T1865	7188-7189	SYM	denotes	−
T1866	7190-7195	NN	denotes	mouse
T1867	7196-7202	NN	denotes	embryo
T1868	7203-7214	NNS	denotes	fibroblasts
T1869	7215-7216	-LRB-	denotes	(
T1870	7216-7220	NNS	denotes	MEFs
T1871	7220-7221	-RRB-	denotes	)
T1872	7222-7226	VBD	denotes	were
T1860	7227-7235	VBN	denotes	impaired
T1873	7236-7238	IN	denotes	in
T1874	7239-7244	PRP$	denotes	their
T1875	7245-7253	NN	denotes	capacity
T1876	7254-7256	TO	denotes	to
T1877	7257-7270	VB	denotes	differentiate
T1878	7271-7275	IN	denotes	into
T1879	7276-7286	NNS	denotes	adipocytes
T1880	7286-7288	,	denotes	,
T1881	7288-7298	JJ	denotes	consistent
T1882	7299-7303	IN	denotes	with
T1883	7304-7309	NN	denotes	Sam68
T1884	7310-7315	VBG	denotes	being
T1885	7316-7317	DT	denotes	a
T1886	7318-7327	NN	denotes	regulator
T1887	7328-7330	IN	denotes	of
T1888	7331-7335	NN	denotes	bone
T1889	7336-7342	NN	denotes	marrow
T1891	7343-7354	JJ	denotes	mesenchymal
T1892	7355-7359	NN	denotes	cell
T1890	7360-7375	NN	denotes	differentiation
T1893	7375-7376	.	denotes	.
T1894	7376-7492	sentence	denotes	These results also characterize a new animal model to study bone metabolism, regeneration, and repair during aging.
T1895	7377-7382	DT	denotes	These
T1896	7383-7390	NNS	denotes	results
T1898	7391-7395	RB	denotes	also
T1897	7396-7408	VBP	denotes	characterize
T1899	7409-7410	DT	denotes	a
T1901	7411-7414	JJ	denotes	new
T1902	7415-7421	NN	denotes	animal
T1900	7422-7427	NN	denotes	model
T1903	7428-7430	TO	denotes	to
T1904	7431-7436	VB	denotes	study
T1905	7437-7441	NN	denotes	bone
T1906	7442-7452	NN	denotes	metabolism
T1907	7452-7454	,	denotes	,
T1908	7454-7466	NN	denotes	regeneration
T1909	7466-7468	,	denotes	,
T1910	7468-7471	CC	denotes	and
T1911	7472-7478	NN	denotes	repair
T1912	7479-7485	IN	denotes	during
T1913	7486-7491	NN	denotes	aging
T1914	7491-7492	.	denotes	.
T2061	7503-7508	NN	denotes	Sam68
T2063	7509-7511	VBZ	denotes	Is
T2062	7512-7521	VBN	denotes	Expressed
T2064	7522-7524	IN	denotes	in
T2065	7525-7528	DT	denotes	the
T2067	7529-7539	VBG	denotes	Developing
T2066	7540-7548	NN	denotes	Skeleton
T2068	7549-7551	IN	denotes	of
T2069	7552-7561	JJ	denotes	Embryonic
T2070	7562-7566	NNS	denotes	Mice
T2071	7566-7692	sentence	denotes	The Sam68 mRNA is known to be widely expressed [38], whereas its pattern of protein expression in vivo remains to be defined.
T2072	7567-7570	DT	denotes	The
T2074	7571-7576	NN	denotes	Sam68
T2073	7577-7581	NN	denotes	mRNA
T2076	7582-7584	VBZ	denotes	is
T2075	7585-7590	VBN	denotes	known
T2077	7591-7593	TO	denotes	to
T2079	7594-7596	VB	denotes	be
T2080	7597-7603	RB	denotes	widely
T2078	7604-7613	VBN	denotes	expressed
T2081	7614-7615	-LRB-	denotes	[
T2082	7615-7617	CD	denotes	38
T2083	7617-7618	-RRB-	denotes	]
T2084	7618-7620	,	denotes	,
T2085	7620-7627	IN	denotes	whereas
T2087	7628-7631	PRP$	denotes	its
T2088	7632-7639	NN	denotes	pattern
T2089	7640-7642	IN	denotes	of
T2090	7643-7650	NN	denotes	protein
T2091	7651-7661	NN	denotes	expression
T2092	7662-7664	FW	denotes	in
T2093	7665-7669	FW	denotes	vivo
T2086	7670-7677	VBZ	denotes	remains
T2094	7678-7680	TO	denotes	to
T2096	7681-7683	VB	denotes	be
T2095	7684-7691	VBN	denotes	defined
T2097	7691-7692	.	denotes	.
T2098	7692-7959	sentence	denotes	Sections of paraffin-embedded wild-type E14.5 and E16.5 embryonic mice were immunostained with the well-characterized AD1 anti-Sam68 antibody, raised in rabbits against an immunizing peptide that corresponds to amino acids 330 to 348 of the mouse Sam68 protein [46].
T2099	7693-7701	NNS	denotes	Sections
T2101	7702-7704	IN	denotes	of
T2102	7705-7713	NN	denotes	paraffin
T2104	7713-7714	HYPH	denotes	-
T2103	7714-7722	VBN	denotes	embedded
T2106	7723-7727	JJ	denotes	wild
T2108	7727-7728	HYPH	denotes	-
T2107	7728-7732	NN	denotes	type
T2109	7733-7738	NN	denotes	E14.5
T2110	7739-7742	CC	denotes	and
T2111	7743-7748	NN	denotes	E16.5
T2112	7749-7758	JJ	denotes	embryonic
T2105	7759-7763	NNS	denotes	mice
T2113	7764-7768	VBD	denotes	were
T2100	7769-7782	VBN	denotes	immunostained
T2114	7783-7787	IN	denotes	with
T2115	7788-7791	DT	denotes	the
T2117	7792-7796	RB	denotes	well
T2119	7796-7797	HYPH	denotes	-
T2118	7797-7810	VBN	denotes	characterized
T2120	7811-7814	NN	denotes	AD1
T2121	7815-7825	JJ	denotes	anti-Sam68
T2116	7826-7834	NN	denotes	antibody
T2122	7834-7836	,	denotes	,
T2123	7836-7842	VBN	denotes	raised
T2124	7843-7845	IN	denotes	in
T2125	7846-7853	NNS	denotes	rabbits
T2126	7854-7861	IN	denotes	against
T2127	7862-7864	DT	denotes	an
T2129	7865-7875	VBG	denotes	immunizing
T2128	7876-7883	NN	denotes	peptide
T2130	7884-7888	WDT	denotes	that
T2131	7889-7900	VBZ	denotes	corresponds
T2132	7901-7903	IN	denotes	to
T2133	7904-7909	NN	denotes	amino
T2135	7910-7915	NNS	denotes	acids
T2134	7916-7919	CD	denotes	330
T2136	7920-7922	IN	denotes	to
T2137	7923-7926	CD	denotes	348
T2138	7927-7929	IN	denotes	of
T2139	7930-7933	DT	denotes	the
T2141	7934-7939	NN	denotes	mouse
T2142	7940-7945	NN	denotes	Sam68
T2140	7946-7953	NN	denotes	protein
T2143	7954-7955	-LRB-	denotes	[
T2144	7955-7957	CD	denotes	46
T2145	7957-7958	-RRB-	denotes	]
T2146	7958-7959	.	denotes	.
T2147	7959-8088	sentence	denotes	Sam68 immunoreactivity was observed in the skeleton and soft tissues of developing wild-type mice at E14.5 and E16.5 (Figure 1).
T2148	7960-7965	NN	denotes	Sam68
T2149	7966-7982	NN	denotes	immunoreactivity
T2151	7983-7986	VBD	denotes	was
T2150	7987-7995	VBN	denotes	observed
T2152	7996-7998	IN	denotes	in
T2153	7999-8002	DT	denotes	the
T2154	8003-8011	NN	denotes	skeleton
T2155	8012-8015	CC	denotes	and
T2156	8016-8020	JJ	denotes	soft
T2157	8021-8028	NNS	denotes	tissues
T2158	8029-8031	IN	denotes	of
T2159	8032-8042	VBG	denotes	developing
T2161	8043-8047	JJ	denotes	wild
T2163	8047-8048	HYPH	denotes	-
T2162	8048-8052	NN	denotes	type
T2160	8053-8057	NNS	denotes	mice
T2164	8058-8060	IN	denotes	at
T2165	8061-8066	NN	denotes	E14.5
T2166	8067-8070	CC	denotes	and
T2167	8071-8076	NN	denotes	E16.5
T2168	8077-8078	-LRB-	denotes	(
T2169	8078-8084	NN	denotes	Figure
T2170	8085-8086	CD	denotes	1
T2171	8086-8087	-RRB-	denotes	)
T2172	8087-8088	.	denotes	.
T2173	8088-8460	sentence	denotes	Intense staining was seen in the nucleus of cells in the developing brain, heart, and small intestine (Figure 1B), as well as in chondrocytes in the nasal septum and the glandular tissue adjacent to the nasal cartilage (Figure 1C, panels A–D), in the vertebra and intervertebral discs (panels E–H) and in the epiphysis (panels I–K) and metaphysis (panel L) of long bones.
T2174	8089-8096	JJ	denotes	Intense
T2175	8097-8105	NN	denotes	staining
T2177	8106-8109	VBD	denotes	was
T2176	8110-8114	VBN	denotes	seen
T2178	8115-8117	IN	denotes	in
T2179	8118-8121	DT	denotes	the
T2180	8122-8129	NN	denotes	nucleus
T2181	8130-8132	IN	denotes	of
T2182	8133-8138	NNS	denotes	cells
T2183	8139-8141	IN	denotes	in
T2184	8142-8145	DT	denotes	the
T2186	8146-8156	VBG	denotes	developing
T2185	8157-8162	NN	denotes	brain
T2187	8162-8164	,	denotes	,
T2188	8164-8169	NN	denotes	heart
T2189	8169-8171	,	denotes	,
T2190	8171-8174	CC	denotes	and
T2191	8175-8180	JJ	denotes	small
T2192	8181-8190	NN	denotes	intestine
T2193	8191-8192	-LRB-	denotes	(
T2195	8192-8198	NN	denotes	Figure
T2194	8199-8201	NN	denotes	1B
T2196	8201-8202	-RRB-	denotes	)
T2197	8202-8204	,	denotes	,
T2198	8204-8206	RB	denotes	as
T2200	8207-8211	RB	denotes	well
T2199	8212-8214	IN	denotes	as
T2201	8215-8217	IN	denotes	in
T2202	8218-8230	NNS	denotes	chondrocytes
T2203	8231-8233	IN	denotes	in
T2204	8234-8237	DT	denotes	the
T2206	8238-8243	JJ	denotes	nasal
T2205	8244-8250	NN	denotes	septum
T2207	8251-8254	CC	denotes	and
T2208	8255-8258	DT	denotes	the
T2210	8259-8268	JJ	denotes	glandular
T2209	8269-8275	NN	denotes	tissue
T2211	8276-8284	JJ	denotes	adjacent
T2212	8285-8287	IN	denotes	to
T2213	8288-8291	DT	denotes	the
T2215	8292-8297	JJ	denotes	nasal
T2214	8298-8307	NN	denotes	cartilage
T2216	8308-8309	-LRB-	denotes	(
T2218	8309-8315	NN	denotes	Figure
T2217	8316-8318	NN	denotes	1C
T2219	8318-8320	,	denotes	,
T2220	8320-8326	NNS	denotes	panels
T2221	8327-8328	NN	denotes	A
T2222	8328-8329	SYM	denotes	–
T2223	8329-8330	NN	denotes	D
T2224	8330-8331	-RRB-	denotes	)
T2225	8331-8333	,	denotes	,
T2226	8333-8335	IN	denotes	in
T2227	8336-8339	DT	denotes	the
T2228	8340-8348	NNS	denotes	vertebra
T2229	8349-8352	CC	denotes	and
T2230	8353-8367	JJ	denotes	intervertebral
T2231	8368-8373	NNS	denotes	discs
T2232	8374-8375	-LRB-	denotes	(
T2234	8375-8381	NNS	denotes	panels
T2233	8382-8383	NN	denotes	E
T2235	8383-8384	SYM	denotes	–
T2236	8384-8385	NN	denotes	H
T2237	8385-8386	-RRB-	denotes	)
T2238	8387-8390	CC	denotes	and
T2239	8391-8393	IN	denotes	in
T2240	8394-8397	DT	denotes	the
T2241	8398-8407	NN	denotes	epiphysis
T2242	8408-8409	-LRB-	denotes	(
T2244	8409-8415	NNS	denotes	panels
T2243	8416-8417	NN	denotes	I
T2245	8417-8418	SYM	denotes	–
T2246	8418-8419	NN	denotes	K
T2247	8419-8420	-RRB-	denotes	)
T2248	8421-8424	CC	denotes	and
T2249	8425-8435	NN	denotes	metaphysis
T2250	8436-8437	-LRB-	denotes	(
T2252	8437-8442	NN	denotes	panel
T2251	8443-8444	NN	denotes	L
T2253	8444-8445	-RRB-	denotes	)
T2254	8446-8448	IN	denotes	of
T2255	8449-8453	JJ	denotes	long
T2256	8454-8459	NNS	denotes	bones
T2257	8459-8460	.	denotes	.
T2258	8460-8643	sentence	denotes	Nuclear staining was observed in proliferating chondrocytes and hypertrophic chondrocytes (Figure 1C, panel H), in osteoblasts (panel L; Dataset S1), and in osteoclasts (Dataset S1).
T2259	8461-8468	JJ	denotes	Nuclear
T2260	8469-8477	NN	denotes	staining
T2262	8478-8481	VBD	denotes	was
T2261	8482-8490	VBN	denotes	observed
T2263	8491-8493	IN	denotes	in
T2264	8494-8507	VBG	denotes	proliferating
T2265	8508-8520	NNS	denotes	chondrocytes
T2266	8521-8524	CC	denotes	and
T2267	8525-8537	JJ	denotes	hypertrophic
T2268	8538-8550	NNS	denotes	chondrocytes
T2269	8551-8552	-LRB-	denotes	(
T2271	8552-8558	NN	denotes	Figure
T2270	8559-8561	NN	denotes	1C
T2272	8561-8563	,	denotes	,
T2273	8563-8568	NN	denotes	panel
T2274	8569-8570	NN	denotes	H
T2275	8570-8571	-RRB-	denotes	)
T2276	8571-8573	,	denotes	,
T2277	8573-8575	IN	denotes	in
T2278	8576-8587	NNS	denotes	osteoblasts
T2279	8588-8589	-LRB-	denotes	(
T2281	8589-8594	NN	denotes	panel
T2280	8595-8596	NN	denotes	L
T2282	8596-8597	:	denotes	;
T2283	8598-8605	NN	denotes	Dataset
T2284	8606-8608	NN	denotes	S1
T2285	8608-8609	-RRB-	denotes	)
T2286	8609-8611	,	denotes	,
T2287	8611-8614	CC	denotes	and
T2288	8615-8617	IN	denotes	in
T2289	8618-8629	NNS	denotes	osteoclasts
T2290	8630-8631	-LRB-	denotes	(
T2292	8631-8638	NN	denotes	Dataset
T2291	8639-8641	NN	denotes	S1
T2293	8641-8642	-RRB-	denotes	)
T2294	8642-8643	.	denotes	.
T2295	8643-8788	sentence	denotes	No staining was observed with preimmune serum or when the antiserum was preadsorbed with the immunizing peptide (Figure 1C, panels B, F, and J).
T2296	8644-8646	DT	denotes	No
T2297	8647-8655	NN	denotes	staining
T2299	8656-8659	VBD	denotes	was
T2298	8660-8668	VBN	denotes	observed
T2300	8669-8673	IN	denotes	with
T2301	8674-8683	JJ	denotes	preimmune
T2302	8684-8689	NN	denotes	serum
T2303	8690-8692	CC	denotes	or
T2304	8693-8697	WRB	denotes	when
T2306	8698-8701	DT	denotes	the
T2307	8702-8711	NN	denotes	antiserum
T2308	8712-8715	VBD	denotes	was
T2305	8716-8727	VBN	denotes	preadsorbed
T2309	8728-8732	IN	denotes	with
T2310	8733-8736	DT	denotes	the
T2312	8737-8747	VBG	denotes	immunizing
T2311	8748-8755	NN	denotes	peptide
T2313	8756-8757	-LRB-	denotes	(
T2315	8757-8763	NN	denotes	Figure
T2314	8764-8766	NN	denotes	1C
T2316	8766-8768	,	denotes	,
T2317	8768-8774	NNS	denotes	panels
T2318	8775-8776	NN	denotes	B
T2319	8776-8778	,	denotes	,
T2320	8778-8779	NN	denotes	F
T2321	8779-8781	,	denotes	,
T2322	8781-8784	CC	denotes	and
T2323	8785-8786	NN	denotes	J
T2324	8786-8787	-RRB-	denotes	)
T2325	8787-8788	.	denotes	.
T2326	8788-8964	sentence	denotes	Taken together, these data demonstrate that Sam68 protein is selectively expressed in the developing mouse embryo, with particularly elevated expression in cartilage and bone.
T2327	8789-8794	VBN	denotes	Taken
T2329	8795-8803	RB	denotes	together
T2330	8803-8805	,	denotes	,
T2331	8805-8810	DT	denotes	these
T2332	8811-8815	NNS	denotes	data
T2328	8816-8827	VBP	denotes	demonstrate
T2333	8828-8832	IN	denotes	that
T2335	8833-8838	NN	denotes	Sam68
T2336	8839-8846	NN	denotes	protein
T2337	8847-8849	VBZ	denotes	is
T2338	8850-8861	RB	denotes	selectively
T2334	8862-8871	VBN	denotes	expressed
T2339	8872-8874	IN	denotes	in
T2340	8875-8878	DT	denotes	the
T2342	8879-8889	VBG	denotes	developing
T2343	8890-8895	NN	denotes	mouse
T2341	8896-8902	NN	denotes	embryo
T2344	8902-8904	,	denotes	,
T2345	8904-8908	IN	denotes	with
T2346	8909-8921	RB	denotes	particularly
T2347	8922-8930	VBN	denotes	elevated
T2348	8931-8941	NN	denotes	expression
T2349	8942-8944	IN	denotes	in
T2350	8945-8954	NN	denotes	cartilage
T2351	8955-8958	CC	denotes	and
T2352	8959-8963	NN	denotes	bone
T2353	8963-8964	.	denotes	.
T2520	10165-10170	NN	denotes	Sam68
T2521	10171-10173	VBZ	denotes	is
T2522	10174-10177	RB	denotes	Not
T2523	10178-10187	JJ	denotes	Essential
T2524	10188-10191	IN	denotes	for
T2525	10192-10197	NN	denotes	Mouse
T2526	10198-10209	NN	denotes	Development
T2527	10209-10425	sentence	denotes	To define the physiologic role of Sam68, we generated Sam68-deficient mice by targeted disruption of exons 4 and 5 of the sam68 gene, which encode the functional region of the KH-type RNA binding domain (Figure 2A).
T2528	10210-10212	TO	denotes	To
T2529	10213-10219	VB	denotes	define
T2531	10220-10223	DT	denotes	the
T2533	10224-10235	JJ	denotes	physiologic
T2532	10236-10240	NN	denotes	role
T2534	10241-10243	IN	denotes	of
T2535	10244-10249	NN	denotes	Sam68
T2536	10249-10251	,	denotes	,
T2537	10251-10253	PRP	denotes	we
T2530	10254-10263	VBD	denotes	generated
T2538	10264-10269	NN	denotes	Sam68
T2540	10269-10270	HYPH	denotes	-
T2539	10270-10279	JJ	denotes	deficient
T2541	10280-10284	NNS	denotes	mice
T2542	10285-10287	IN	denotes	by
T2543	10288-10296	VBN	denotes	targeted
T2544	10297-10307	NN	denotes	disruption
T2545	10308-10310	IN	denotes	of
T2546	10311-10316	NNS	denotes	exons
T2547	10317-10318	CD	denotes	4
T2548	10319-10322	CC	denotes	and
T2549	10323-10324	CD	denotes	5
T2550	10325-10327	IN	denotes	of
T2551	10328-10331	DT	denotes	the
T2553	10332-10337	NN	denotes	sam68
T2552	10338-10342	NN	denotes	gene
T2554	10342-10344	,	denotes	,
T2555	10344-10349	WDT	denotes	which
T2556	10350-10356	VBP	denotes	encode
T2557	10357-10360	DT	denotes	the
T2559	10361-10371	JJ	denotes	functional
T2558	10372-10378	NN	denotes	region
T2560	10379-10381	IN	denotes	of
T2561	10382-10385	DT	denotes	the
T2563	10386-10388	NN	denotes	KH
T2565	10388-10389	HYPH	denotes	-
T2564	10389-10393	NN	denotes	type
T2566	10394-10397	NN	denotes	RNA
T2567	10398-10405	NN	denotes	binding
T2562	10406-10412	NN	denotes	domain
T2568	10413-10414	-LRB-	denotes	(
T2570	10414-10420	NN	denotes	Figure
T2569	10421-10423	NN	denotes	2A
T2571	10423-10424	-RRB-	denotes	)
T2572	10424-10425	.	denotes	.
T2573	10425-10559	sentence	denotes	The integrity of the targeted allele was verified by Southern blot analysis (Figure 2B) and by PCR of genomic DNA (unpublished data).
T2574	10426-10429	DT	denotes	The
T2575	10430-10439	NN	denotes	integrity
T2577	10440-10442	IN	denotes	of
T2578	10443-10446	DT	denotes	the
T2580	10447-10455	VBN	denotes	targeted
T2579	10456-10462	NN	denotes	allele
T2581	10463-10466	VBD	denotes	was
T2576	10467-10475	VBN	denotes	verified
T2582	10476-10478	IN	denotes	by
T2583	10479-10487	NNP	denotes	Southern
T2584	10488-10492	NN	denotes	blot
T2585	10493-10501	NN	denotes	analysis
T2586	10502-10503	-LRB-	denotes	(
T2588	10503-10509	NN	denotes	Figure
T2587	10510-10512	NN	denotes	2B
T2589	10512-10513	-RRB-	denotes	)
T2590	10514-10517	CC	denotes	and
T2591	10518-10520	IN	denotes	by
T2592	10521-10524	NN	denotes	PCR
T2593	10525-10527	IN	denotes	of
T2594	10528-10535	JJ	denotes	genomic
T2595	10536-10539	NN	denotes	DNA
T2596	10540-10541	-LRB-	denotes	(
T2598	10541-10552	JJ	denotes	unpublished
T2597	10553-10557	NNS	denotes	data
T2599	10557-10558	-RRB-	denotes	)
T2600	10558-10559	.	denotes	.
T2601	10559-10859	sentence	denotes	Sam68 transcripts encoded by exons 1 to 5 were absent as evidenced by RT-PCR (Dataset S2), and Sam68−/− mice were devoid of Sam68 protein, as visualized by immunoblotting with anti-Sam68 AD1 and SC-333 antibodies or control normal rabbit serum or anti-Sam68 AD1 preabsorbed with peptide (Figure 2C).
T2602	10560-10565	NN	denotes	Sam68
T2603	10566-10577	NNS	denotes	transcripts
T2605	10578-10585	VBN	denotes	encoded
T2606	10586-10588	IN	denotes	by
T2607	10589-10594	NNS	denotes	exons
T2608	10595-10596	CD	denotes	1
T2609	10597-10599	IN	denotes	to
T2610	10600-10601	CD	denotes	5
T2604	10602-10606	VBD	denotes	were
T2611	10607-10613	JJ	denotes	absent
T2612	10614-10616	IN	denotes	as
T2613	10617-10626	VBN	denotes	evidenced
T2614	10627-10629	IN	denotes	by
T2615	10630-10632	NN	denotes	RT
T2617	10632-10633	HYPH	denotes	-
T2616	10633-10636	NN	denotes	PCR
T2618	10637-10638	-LRB-	denotes	(
T2620	10638-10645	NN	denotes	Dataset
T2619	10646-10648	NN	denotes	S2
T2621	10648-10649	-RRB-	denotes	)
T2622	10649-10651	,	denotes	,
T2623	10651-10654	CC	denotes	and
T2624	10655-10660	NN	denotes	Sam68
T2626	10660-10661	SYM	denotes	−
T2627	10661-10662	HYPH	denotes	/
T2628	10662-10663	SYM	denotes	−
T2625	10664-10668	NNS	denotes	mice
T2629	10669-10673	VBD	denotes	were
T2630	10674-10680	JJ	denotes	devoid
T2631	10681-10683	IN	denotes	of
T2632	10684-10689	NN	denotes	Sam68
T2633	10690-10697	NN	denotes	protein
T2634	10697-10699	,	denotes	,
T2635	10699-10701	IN	denotes	as
T2636	10702-10712	VBN	denotes	visualized
T2637	10713-10715	IN	denotes	by
T2638	10716-10730	VBG	denotes	immunoblotting
T2639	10731-10735	IN	denotes	with
T2640	10736-10746	JJ	denotes	anti-Sam68
T2641	10747-10750	NN	denotes	AD1
T2643	10751-10754	CC	denotes	and
T2644	10755-10757	NN	denotes	SC
T2645	10757-10758	HYPH	denotes	-
T2646	10758-10761	CD	denotes	333
T2642	10762-10772	NNS	denotes	antibodies
T2647	10773-10775	CC	denotes	or
T2648	10776-10783	NN	denotes	control
T2650	10784-10790	JJ	denotes	normal
T2651	10791-10797	NN	denotes	rabbit
T2649	10798-10803	NN	denotes	serum
T2652	10804-10806	CC	denotes	or
T2653	10807-10817	JJ	denotes	anti-Sam68
T2654	10818-10821	NN	denotes	AD1
T2655	10822-10833	VBN	denotes	preabsorbed
T2656	10834-10838	IN	denotes	with
T2657	10839-10846	NN	denotes	peptide
T2658	10847-10848	-LRB-	denotes	(
T2660	10848-10854	NN	denotes	Figure
T2659	10855-10857	NN	denotes	2C
T2661	10857-10858	-RRB-	denotes	)
T2662	10858-10859	.	denotes	.
T2663	10859-10967	sentence	denotes	SC-333 is a rabbit anti-Sam68 antibody that was raised against the C-terminal 20 amino acids of Sam68 [47].
T2664	10860-10862	NN	denotes	SC
T2666	10862-10863	HYPH	denotes	-
T2667	10863-10866	CD	denotes	333
T2665	10867-10869	VBZ	denotes	is
T2668	10870-10871	DT	denotes	a
T2670	10872-10878	NN	denotes	rabbit
T2671	10879-10889	JJ	denotes	anti-Sam68
T2669	10890-10898	NN	denotes	antibody
T2672	10899-10903	WDT	denotes	that
T2674	10904-10907	VBD	denotes	was
T2673	10908-10914	VBN	denotes	raised
T2675	10915-10922	IN	denotes	against
T2676	10923-10926	DT	denotes	the
T2678	10927-10928	NN	denotes	C
T2680	10928-10929	HYPH	denotes	-
T2679	10929-10937	JJ	denotes	terminal
T2681	10938-10940	CD	denotes	20
T2682	10941-10946	NN	denotes	amino
T2677	10947-10952	NNS	denotes	acids
T2683	10953-10955	IN	denotes	of
T2684	10956-10961	NN	denotes	Sam68
T2685	10962-10963	-LRB-	denotes	[
T2686	10963-10965	CD	denotes	47
T2687	10965-10966	-RRB-	denotes	]
T2688	10966-10967	.	denotes	.
T2689	10967-11034	sentence	denotes	These data confirmed the generation of a mouse deficient in Sam68.
T2690	10968-10973	DT	denotes	These
T2691	10974-10978	NNS	denotes	data
T2692	10979-10988	VBD	denotes	confirmed
T2693	10989-10992	DT	denotes	the
T2694	10993-11003	NN	denotes	generation
T2695	11004-11006	IN	denotes	of
T2696	11007-11008	DT	denotes	a
T2697	11009-11014	NN	denotes	mouse
T2698	11015-11024	JJ	denotes	deficient
T2699	11025-11027	IN	denotes	in
T2700	11028-11033	NN	denotes	Sam68
T2701	11033-11034	.	denotes	.
T2702	11034-11146	sentence	denotes	The genotypes of offspring from heterozygote intercrosses exhibited a Mendelian segregation at E18.5 (Table 1).
T2703	11035-11038	DT	denotes	The
T2704	11039-11048	NNS	denotes	genotypes
T2706	11049-11051	IN	denotes	of
T2707	11052-11061	NN	denotes	offspring
T2708	11062-11066	IN	denotes	from
T2709	11067-11079	NN	denotes	heterozygote
T2710	11080-11092	NNS	denotes	intercrosses
T2705	11093-11102	VBD	denotes	exhibited
T2711	11103-11104	DT	denotes	a
T2713	11105-11114	JJ	denotes	Mendelian
T2712	11115-11126	NN	denotes	segregation
T2714	11127-11129	IN	denotes	at
T2715	11130-11135	NN	denotes	E18.5
T2716	11136-11137	-LRB-	denotes	(
T2717	11137-11142	NN	denotes	Table
T2718	11143-11144	CD	denotes	1
T2719	11144-11145	-RRB-	denotes	)
T2720	11145-11146	.	denotes	.
T2721	11146-11244	sentence	denotes	Despite the lack of visible deformity, many of the Sam68−/− pups died at birth of unknown causes.
T2722	11147-11154	IN	denotes	Despite
T2724	11155-11158	DT	denotes	the
T2725	11159-11163	NN	denotes	lack
T2726	11164-11166	IN	denotes	of
T2727	11167-11174	JJ	denotes	visible
T2728	11175-11184	NN	denotes	deformity
T2729	11184-11186	,	denotes	,
T2730	11186-11190	JJ	denotes	many
T2731	11191-11193	IN	denotes	of
T2732	11194-11197	DT	denotes	the
T2734	11198-11203	NN	denotes	Sam68
T2735	11203-11204	SYM	denotes	−
T2736	11204-11205	HYPH	denotes	/
T2737	11205-11206	SYM	denotes	−
T2733	11207-11211	NNS	denotes	pups
T2723	11212-11216	VBD	denotes	died
T2738	11217-11219	IN	denotes	at
T2739	11220-11225	NN	denotes	birth
T2740	11226-11228	IN	denotes	of
T2741	11229-11236	JJ	denotes	unknown
T2742	11237-11243	NNS	denotes	causes
T2743	11243-11244	.	denotes	.
T2744	11244-11367	sentence	denotes	Sam68+/- mice were phenotypically normal and Sam68−/− pups that survived the perinatal period invariably lived to old age.
T2745	11245-11250	NN	denotes	Sam68
T2747	11250-11251	SYM	denotes	+
T2748	11251-11252	HYPH	denotes	/
T2749	11252-11253	SYM	denotes	-
T2746	11254-11258	NNS	denotes	mice
T2750	11259-11263	VBD	denotes	were
T2751	11264-11278	RB	denotes	phenotypically
T2752	11279-11285	JJ	denotes	normal
T2753	11286-11289	CC	denotes	and
T2754	11290-11295	NN	denotes	Sam68
T2756	11295-11296	SYM	denotes	−
T2757	11296-11297	HYPH	denotes	/
T2758	11297-11298	SYM	denotes	−
T2755	11299-11303	NNS	denotes	pups
T2760	11304-11308	WDT	denotes	that
T2761	11309-11317	VBD	denotes	survived
T2762	11318-11321	DT	denotes	the
T2764	11322-11331	JJ	denotes	perinatal
T2763	11332-11338	NN	denotes	period
T2765	11339-11349	RB	denotes	invariably
T2759	11350-11355	VBD	denotes	lived
T2766	11356-11358	IN	denotes	to
T2767	11359-11362	JJ	denotes	old
T2768	11363-11366	NN	denotes	age
T2769	11366-11367	.	denotes	.
T2770	11367-11552	sentence	denotes	Despite evidence that Sam68 mRNA is widely expressed and phosphorylated in mitosis [41,42], the Sam68−/− mice did not develop tumors and showed no immunologic or other major illnesses.
T2771	11368-11375	IN	denotes	Despite
T2773	11376-11384	NN	denotes	evidence
T2774	11385-11389	IN	denotes	that
T2776	11390-11395	NN	denotes	Sam68
T2777	11396-11400	NN	denotes	mRNA
T2778	11401-11403	VBZ	denotes	is
T2779	11404-11410	RB	denotes	widely
T2775	11411-11420	VBN	denotes	expressed
T2780	11421-11424	CC	denotes	and
T2781	11425-11439	VBN	denotes	phosphorylated
T2782	11440-11442	IN	denotes	in
T2783	11443-11450	NN	denotes	mitosis
T2784	11451-11452	-LRB-	denotes	[
T2786	11452-11454	CD	denotes	41
T2787	11454-11455	,	denotes	,
T2785	11455-11457	CD	denotes	42
T2788	11457-11458	-RRB-	denotes	]
T2789	11458-11460	,	denotes	,
T2790	11460-11463	DT	denotes	the
T2792	11464-11469	NN	denotes	Sam68
T2793	11469-11470	SYM	denotes	−
T2794	11470-11471	HYPH	denotes	/
T2795	11471-11472	SYM	denotes	−
T2791	11473-11477	NNS	denotes	mice
T2796	11478-11481	VBD	denotes	did
T2797	11482-11485	RB	denotes	not
T2772	11486-11493	VB	denotes	develop
T2798	11494-11500	NNS	denotes	tumors
T2799	11501-11504	CC	denotes	and
T2800	11505-11511	VBD	denotes	showed
T2801	11512-11514	DT	denotes	no
T2803	11515-11526	JJ	denotes	immunologic
T2804	11527-11529	CC	denotes	or
T2805	11530-11535	JJ	denotes	other
T2806	11536-11541	JJ	denotes	major
T2802	11542-11551	NNS	denotes	illnesses
T2807	11551-11552	.	denotes	.
T2808	11552-11691	sentence	denotes	Sam68−/− mice did, however, have difficulty breeding due to male infertility and the females rarely provided adequate care to their young.
T2809	11553-11558	NN	denotes	Sam68
T2811	11558-11559	SYM	denotes	−
T2812	11559-11560	HYPH	denotes	/
T2813	11560-11561	SYM	denotes	−
T2810	11562-11566	NNS	denotes	mice
T2815	11567-11570	VBD	denotes	did
T2816	11570-11572	,	denotes	,
T2817	11572-11579	RB	denotes	however
T2818	11579-11581	,	denotes	,
T2814	11581-11585	VB	denotes	have
T2819	11586-11596	NN	denotes	difficulty
T2820	11597-11605	VBG	denotes	breeding
T2821	11606-11609	IN	denotes	due
T2822	11610-11612	IN	denotes	to
T2823	11613-11617	JJ	denotes	male
T2824	11618-11629	NN	denotes	infertility
T2825	11630-11633	CC	denotes	and
T2826	11634-11637	DT	denotes	the
T2827	11638-11645	NNS	denotes	females
T2829	11646-11652	RB	denotes	rarely
T2828	11653-11661	VBD	denotes	provided
T2830	11662-11670	JJ	denotes	adequate
T2831	11671-11675	NN	denotes	care
T2832	11676-11678	IN	denotes	to
T2833	11679-11684	PRP$	denotes	their
T2834	11685-11690	JJ	denotes	young
T2835	11690-11691	.	denotes	.
T3035	12920-12925	NN	denotes	Sam68
T3036	12926-12936	NN	denotes	Deficiency
T3037	12937-12945	VBZ	denotes	Protects
T3038	12946-12950	NNS	denotes	Mice
T3039	12951-12955	IN	denotes	from
T3040	12956-12959	NN	denotes	Age
T3042	12959-12960	HYPH	denotes	-
T3041	12960-12967	VBN	denotes	Related
T3044	12968-12972	NN	denotes	Bone
T3043	12973-12977	NN	denotes	Loss
T3045	12977-13146	sentence	denotes	Src null animals are known to have bone metabolism defects [48], and since Sam68 is a substrate of Src, we decided to analyze the Sam68 mice for skeletal abnormalities.
T3046	12978-12981	NN	denotes	Src
T3048	12982-12986	JJ	denotes	null
T3047	12987-12994	NNS	denotes	animals
T3050	12995-12998	VBP	denotes	are
T3049	12999-13004	VBN	denotes	known
T3051	13005-13007	TO	denotes	to
T3052	13008-13012	VB	denotes	have
T3053	13013-13017	NN	denotes	bone
T3054	13018-13028	NN	denotes	metabolism
T3055	13029-13036	NNS	denotes	defects
T3056	13037-13038	-LRB-	denotes	[
T3057	13038-13040	CD	denotes	48
T3058	13040-13041	-RRB-	denotes	]
T3059	13041-13043	,	denotes	,
T3060	13043-13046	CC	denotes	and
T3061	13047-13052	IN	denotes	since
T3063	13053-13058	NN	denotes	Sam68
T3062	13059-13061	VBZ	denotes	is
T3065	13062-13063	DT	denotes	a
T3066	13064-13073	NN	denotes	substrate
T3067	13074-13076	IN	denotes	of
T3068	13077-13080	NN	denotes	Src
T3069	13080-13082	,	denotes	,
T3070	13082-13084	PRP	denotes	we
T3064	13085-13092	VBD	denotes	decided
T3071	13093-13095	TO	denotes	to
T3072	13096-13103	VB	denotes	analyze
T3073	13104-13107	DT	denotes	the
T3075	13108-13113	NN	denotes	Sam68
T3074	13114-13118	NNS	denotes	mice
T3076	13119-13122	IN	denotes	for
T3077	13123-13131	JJ	denotes	skeletal
T3078	13132-13145	NNS	denotes	abnormalities
T3079	13145-13146	.	denotes	.
T3080	13146-13252	sentence	denotes	Cohorts of Sam68+/+ and Sam68−/− mice were euthanized at 4 and 12 months of age for skeletal phenotyping.
T3081	13147-13154	NNS	denotes	Cohorts
T3083	13155-13157	IN	denotes	of
T3084	13158-13163	NN	denotes	Sam68
T3086	13163-13164	SYM	denotes	+
T3087	13164-13165	HYPH	denotes	/
T3088	13165-13166	SYM	denotes	+
T3089	13167-13170	CC	denotes	and
T3090	13171-13176	NN	denotes	Sam68
T3091	13176-13177	SYM	denotes	−
T3092	13177-13178	HYPH	denotes	/
T3093	13178-13179	SYM	denotes	−
T3085	13180-13184	NNS	denotes	mice
T3094	13185-13189	VBD	denotes	were
T3082	13190-13200	VBN	denotes	euthanized
T3095	13201-13203	IN	denotes	at
T3096	13204-13205	CD	denotes	4
T3098	13206-13209	CC	denotes	and
T3099	13210-13212	CD	denotes	12
T3097	13213-13219	NNS	denotes	months
T3100	13220-13222	IN	denotes	of
T3101	13223-13226	NN	denotes	age
T3102	13227-13230	IN	denotes	for
T3103	13231-13239	JJ	denotes	skeletal
T3104	13240-13251	NN	denotes	phenotyping
T3105	13251-13252	.	denotes	.
T3106	13252-13414	sentence	denotes	To minimize differences in the bone phenotype that might arise secondary to gender or weight differences, we selected age-matched female mice for these analyses.
T3107	13253-13255	TO	denotes	To
T3108	13256-13264	VB	denotes	minimize
T3110	13265-13276	NNS	denotes	differences
T3111	13277-13279	IN	denotes	in
T3112	13280-13283	DT	denotes	the
T3114	13284-13288	NN	denotes	bone
T3113	13289-13298	NN	denotes	phenotype
T3115	13299-13303	WDT	denotes	that
T3117	13304-13309	MD	denotes	might
T3116	13310-13315	VB	denotes	arise
T3118	13316-13325	JJ	denotes	secondary
T3119	13326-13328	IN	denotes	to
T3120	13329-13335	NN	denotes	gender
T3122	13336-13338	CC	denotes	or
T3123	13339-13345	NN	denotes	weight
T3121	13346-13357	NNS	denotes	differences
T3124	13357-13359	,	denotes	,
T3125	13359-13361	PRP	denotes	we
T3109	13362-13370	VBD	denotes	selected
T3126	13371-13374	NN	denotes	age
T3128	13374-13375	HYPH	denotes	-
T3127	13375-13382	VBN	denotes	matched
T3130	13383-13389	JJ	denotes	female
T3129	13390-13394	NNS	denotes	mice
T3131	13395-13398	IN	denotes	for
T3132	13399-13404	DT	denotes	these
T3133	13405-13413	NNS	denotes	analyses
T3134	13413-13414	.	denotes	.
T3135	13414-13662	sentence	denotes	The female mice demonstrated similar increases in body weight, although 4-month-old Sam68−/− mice weighed less than Sam68+/+ mice, and similar changes in bone lengths in the axial and appendicular skeleton between 4 and 12 months of age (Table 2).
T3136	13415-13418	DT	denotes	The
T3138	13419-13425	JJ	denotes	female
T3137	13426-13430	NNS	denotes	mice
T3139	13431-13443	VBD	denotes	demonstrated
T3140	13444-13451	JJ	denotes	similar
T3141	13452-13461	NNS	denotes	increases
T3142	13462-13464	IN	denotes	in
T3143	13465-13469	NN	denotes	body
T3144	13470-13476	NN	denotes	weight
T3145	13476-13478	,	denotes	,
T3147	13478-13486	IN	denotes	although
T3148	13487-13488	CD	denotes	4
T3150	13488-13489	HYPH	denotes	-
T3149	13489-13494	NN	denotes	month
T3152	13494-13495	HYPH	denotes	-
T3151	13495-13498	JJ	denotes	old
T3154	13499-13504	NN	denotes	Sam68
T3155	13504-13505	SYM	denotes	−
T3156	13505-13506	HYPH	denotes	/
T3157	13506-13507	SYM	denotes	−
T3153	13508-13512	NNS	denotes	mice
T3146	13513-13520	VBD	denotes	weighed
T3158	13521-13525	JJR	denotes	less
T3159	13526-13530	IN	denotes	than
T3160	13531-13536	NN	denotes	Sam68
T3162	13536-13537	SYM	denotes	+
T3163	13537-13538	HYPH	denotes	/
T3164	13538-13539	SYM	denotes	+
T3161	13540-13544	NNS	denotes	mice
T3165	13544-13546	,	denotes	,
T3166	13546-13549	CC	denotes	and
T3167	13550-13557	JJ	denotes	similar
T3168	13558-13565	NNS	denotes	changes
T3169	13566-13568	IN	denotes	in
T3170	13569-13573	NN	denotes	bone
T3171	13574-13581	NNS	denotes	lengths
T3172	13582-13584	IN	denotes	in
T3173	13585-13588	DT	denotes	the
T3175	13589-13594	JJ	denotes	axial
T3176	13595-13598	CC	denotes	and
T3177	13599-13611	JJ	denotes	appendicular
T3174	13612-13620	NN	denotes	skeleton
T3178	13621-13628	IN	denotes	between
T3179	13629-13630	CD	denotes	4
T3181	13631-13634	CC	denotes	and
T3182	13635-13637	CD	denotes	12
T3180	13638-13644	NNS	denotes	months
T3183	13645-13647	IN	denotes	of
T3184	13648-13651	NN	denotes	age
T3185	13652-13653	-LRB-	denotes	(
T3186	13653-13658	NN	denotes	Table
T3187	13659-13660	CD	denotes	2
T3188	13660-13661	-RRB-	denotes	)
T3189	13661-13662	.	denotes	.
T3190	13662-14033	sentence	denotes	Faxitron radiography (Faxitron X-ray Corporation, Wheeling, Illinois, United States) (Figure 3A) and micro–computed tomography (CT) (Figure 3B) revealed significant cortical thinning (arrow) and a reduction in metaphyseal bone (asterisk) in the distal femora of 12-month-old Sam68+/+ mice compared with 4-month-old mice of either genotype and 12-month-old Sam68−/− mice.
T3191	13663-13671	NNP	denotes	Faxitron
T3192	13672-13683	NN	denotes	radiography
T3194	13684-13685	-LRB-	denotes	(
T3196	13685-13693	NNP	denotes	Faxitron
T3197	13694-13695	NN	denotes	X
T3199	13695-13696	HYPH	denotes	-
T3198	13696-13699	NN	denotes	ray
T3195	13700-13711	NNP	denotes	Corporation
T3200	13711-13713	,	denotes	,
T3201	13713-13721	NNP	denotes	Wheeling
T3202	13721-13723	,	denotes	,
T3203	13723-13731	NNP	denotes	Illinois
T3204	13731-13733	,	denotes	,
T3205	13733-13739	NNP	denotes	United
T3206	13740-13746	NNP	denotes	States
T3207	13746-13747	-RRB-	denotes	)
T3208	13748-13749	-LRB-	denotes	(
T3210	13749-13755	NN	denotes	Figure
T3209	13756-13758	NN	denotes	3A
T3211	13758-13759	-RRB-	denotes	)
T3212	13760-13763	CC	denotes	and
T3213	13764-13778	VBN	denotes	micro–computed
T3214	13779-13789	NN	denotes	tomography
T3215	13790-13791	-LRB-	denotes	(
T3216	13791-13793	NN	denotes	CT
T3217	13793-13794	-RRB-	denotes	)
T3218	13795-13796	-LRB-	denotes	(
T3220	13796-13802	NN	denotes	Figure
T3219	13803-13805	NN	denotes	3B
T3221	13805-13806	-RRB-	denotes	)
T3193	13807-13815	VBD	denotes	revealed
T3222	13816-13827	JJ	denotes	significant
T3224	13828-13836	JJ	denotes	cortical
T3223	13837-13845	NN	denotes	thinning
T3225	13846-13847	-LRB-	denotes	(
T3226	13847-13852	NN	denotes	arrow
T3227	13852-13853	-RRB-	denotes	)
T3228	13854-13857	CC	denotes	and
T3229	13858-13859	DT	denotes	a
T3230	13860-13869	NN	denotes	reduction
T3231	13870-13872	IN	denotes	in
T3232	13873-13884	JJ	denotes	metaphyseal
T3233	13885-13889	NN	denotes	bone
T3234	13890-13891	-LRB-	denotes	(
T3235	13891-13899	NN	denotes	asterisk
T3236	13899-13900	-RRB-	denotes	)
T3237	13901-13903	IN	denotes	in
T3238	13904-13907	DT	denotes	the
T3240	13908-13914	JJ	denotes	distal
T3239	13915-13921	NNS	denotes	femora
T3241	13922-13924	IN	denotes	of
T3242	13925-13927	CD	denotes	12
T3244	13927-13928	HYPH	denotes	-
T3243	13928-13933	NN	denotes	month
T3246	13933-13934	HYPH	denotes	-
T3245	13934-13937	JJ	denotes	old
T3248	13938-13943	NN	denotes	Sam68
T3249	13943-13944	SYM	denotes	+
T3250	13944-13945	HYPH	denotes	/
T3251	13945-13946	SYM	denotes	+
T3247	13947-13951	NNS	denotes	mice
T3252	13952-13960	VBN	denotes	compared
T3253	13961-13965	IN	denotes	with
T3254	13966-13967	CD	denotes	4
T3256	13967-13968	HYPH	denotes	-
T3255	13968-13973	NN	denotes	month
T3258	13973-13974	HYPH	denotes	-
T3257	13974-13977	JJ	denotes	old
T3259	13978-13982	NNS	denotes	mice
T3260	13983-13985	IN	denotes	of
T3261	13986-13992	CC	denotes	either
T3262	13993-14001	NN	denotes	genotype
T3263	14002-14005	CC	denotes	and
T3264	14006-14008	CD	denotes	12
T3266	14008-14009	HYPH	denotes	-
T3265	14009-14014	NN	denotes	month
T3268	14014-14015	HYPH	denotes	-
T3267	14015-14018	JJ	denotes	old
T3270	14019-14024	NN	denotes	Sam68
T3271	14024-14025	SYM	denotes	−
T3272	14025-14026	HYPH	denotes	/
T3273	14026-14027	SYM	denotes	−
T3269	14028-14032	NNS	denotes	mice
T3274	14032-14033	.	denotes	.
T3275	14033-14220	sentence	denotes	Similar reductions in trabecular bone were shown by Faxitron and micro-CT in the fifth lumbar vertebrae of the 12-month-old Sam68+/+ mice but not in the Sam68−/− mice (unpublished data).
T3276	14034-14041	JJ	denotes	Similar
T3277	14042-14052	NNS	denotes	reductions
T3279	14053-14055	IN	denotes	in
T3280	14056-14066	JJ	denotes	trabecular
T3281	14067-14071	NN	denotes	bone
T3282	14072-14076	VBD	denotes	were
T3278	14077-14082	VBN	denotes	shown
T3283	14083-14085	IN	denotes	by
T3284	14086-14094	NNP	denotes	Faxitron
T3285	14095-14098	CC	denotes	and
T3286	14099-14107	NN	denotes	micro-CT
T3287	14108-14110	IN	denotes	in
T3288	14111-14114	DT	denotes	the
T3290	14115-14120	JJ	denotes	fifth
T3291	14121-14127	NN	denotes	lumbar
T3289	14128-14137	NNS	denotes	vertebrae
T3292	14138-14140	IN	denotes	of
T3293	14141-14144	DT	denotes	the
T3295	14145-14147	CD	denotes	12
T3297	14147-14148	HYPH	denotes	-
T3296	14148-14153	NN	denotes	month
T3299	14153-14154	HYPH	denotes	-
T3298	14154-14157	JJ	denotes	old
T3300	14158-14163	NN	denotes	Sam68
T3301	14163-14164	SYM	denotes	+
T3302	14164-14165	HYPH	denotes	/
T3303	14165-14166	SYM	denotes	+
T3294	14167-14171	NNS	denotes	mice
T3304	14172-14175	CC	denotes	but
T3305	14176-14179	RB	denotes	not
T3306	14180-14182	IN	denotes	in
T3307	14183-14186	DT	denotes	the
T3309	14187-14192	NN	denotes	Sam68
T3310	14192-14193	SYM	denotes	−
T3311	14193-14194	HYPH	denotes	/
T3312	14194-14195	SYM	denotes	−
T3308	14196-14200	NNS	denotes	mice
T3313	14201-14202	-LRB-	denotes	(
T3315	14202-14213	JJ	denotes	unpublished
T3314	14214-14218	NNS	denotes	data
T3316	14218-14219	-RRB-	denotes	)
T3317	14219-14220	.	denotes	.
T3318	14220-14503	sentence	denotes	Total body bone mineral content (BMC), quantified with a Lunar PIXImus mouse densitometer (GE-Lunar, Madison, Wisconsin, United States), increased in both Sam68+/+ and Sam68−/− mice between 4 months and 12 months of age, but only reached significance in the Sam68−/− mice (Table 2).
T3319	14221-14226	JJ	denotes	Total
T3321	14227-14231	NN	denotes	body
T3322	14232-14236	NN	denotes	bone
T3323	14237-14244	NN	denotes	mineral
T3320	14245-14252	NN	denotes	content
T3325	14253-14254	-LRB-	denotes	(
T3326	14254-14257	NN	denotes	BMC
T3327	14257-14258	-RRB-	denotes	)
T3328	14258-14260	,	denotes	,
T3329	14260-14270	VBN	denotes	quantified
T3330	14271-14275	IN	denotes	with
T3331	14276-14277	DT	denotes	a
T3333	14278-14283	JJ	denotes	Lunar
T3334	14284-14291	NNP	denotes	PIXImus
T3335	14292-14297	NN	denotes	mouse
T3332	14298-14310	NN	denotes	densitometer
T3336	14311-14312	-LRB-	denotes	(
T3338	14312-14314	NNP	denotes	GE
T3339	14314-14315	HYPH	denotes	-
T3337	14315-14320	NNP	denotes	Lunar
T3340	14320-14322	,	denotes	,
T3341	14322-14329	NNP	denotes	Madison
T3342	14329-14331	,	denotes	,
T3343	14331-14340	NNP	denotes	Wisconsin
T3344	14340-14342	,	denotes	,
T3345	14342-14348	NNP	denotes	United
T3346	14349-14355	NNP	denotes	States
T3347	14355-14356	-RRB-	denotes	)
T3348	14356-14358	,	denotes	,
T3324	14358-14367	VBD	denotes	increased
T3349	14368-14370	IN	denotes	in
T3350	14371-14375	CC	denotes	both
T3351	14376-14381	NN	denotes	Sam68
T3353	14381-14382	SYM	denotes	+
T3354	14382-14383	HYPH	denotes	/
T3355	14383-14384	SYM	denotes	+
T3356	14385-14388	CC	denotes	and
T3357	14389-14394	NN	denotes	Sam68
T3358	14394-14395	SYM	denotes	−
T3359	14395-14396	HYPH	denotes	/
T3360	14396-14397	SYM	denotes	−
T3352	14398-14402	NNS	denotes	mice
T3361	14403-14410	IN	denotes	between
T3362	14411-14412	CD	denotes	4
T3363	14413-14419	NNS	denotes	months
T3364	14420-14423	CC	denotes	and
T3365	14424-14426	CD	denotes	12
T3366	14427-14433	NNS	denotes	months
T3367	14434-14436	IN	denotes	of
T3368	14437-14440	NN	denotes	age
T3369	14440-14442	,	denotes	,
T3370	14442-14445	CC	denotes	but
T3371	14446-14450	RB	denotes	only
T3372	14451-14458	VBD	denotes	reached
T3373	14459-14471	NN	denotes	significance
T3374	14472-14474	IN	denotes	in
T3375	14475-14478	DT	denotes	the
T3377	14479-14484	NN	denotes	Sam68
T3378	14484-14485	SYM	denotes	−
T3379	14485-14486	HYPH	denotes	/
T3380	14486-14487	SYM	denotes	−
T3376	14488-14492	NNS	denotes	mice
T3381	14493-14494	-LRB-	denotes	(
T3382	14494-14499	NN	denotes	Table
T3383	14500-14501	CD	denotes	2
T3384	14501-14502	-RRB-	denotes	)
T3385	14502-14503	.	denotes	.
T3386	14503-14597	sentence	denotes	Similarly, a greater increase in BMC was seen in the femur and vertebra in the Sam68−/− mice.
T3387	14504-14513	RB	denotes	Similarly
T3389	14513-14515	,	denotes	,
T3390	14515-14516	DT	denotes	a
T3392	14517-14524	JJR	denotes	greater
T3391	14525-14533	NN	denotes	increase
T3393	14534-14536	IN	denotes	in
T3394	14537-14540	NN	denotes	BMC
T3395	14541-14544	VBD	denotes	was
T3388	14545-14549	VBN	denotes	seen
T3396	14550-14552	IN	denotes	in
T3397	14553-14556	DT	denotes	the
T3398	14557-14562	NN	denotes	femur
T3399	14563-14566	CC	denotes	and
T3400	14567-14575	NN	denotes	vertebra
T3401	14576-14578	IN	denotes	in
T3402	14579-14582	DT	denotes	the
T3404	14583-14588	NN	denotes	Sam68
T3405	14588-14589	SYM	denotes	−
T3406	14589-14590	HYPH	denotes	/
T3407	14590-14591	SYM	denotes	−
T3403	14592-14596	NNS	denotes	mice
T3408	14596-14597	.	denotes	.
T3409	14597-14803	sentence	denotes	Bone mineral density (BMD) remained constant or decreased in the wild type mice but increased significantly in the total body and in the femoral and vertebral regions of interest in the Sam68−/− (Table 2).
T3410	14598-14602	NN	denotes	Bone
T3411	14603-14610	NN	denotes	mineral
T3412	14611-14618	NN	denotes	density
T3414	14619-14620	-LRB-	denotes	(
T3415	14620-14623	NN	denotes	BMD
T3416	14623-14624	-RRB-	denotes	)
T3413	14625-14633	VBD	denotes	remained
T3417	14634-14642	JJ	denotes	constant
T3418	14643-14645	CC	denotes	or
T3419	14646-14655	VBN	denotes	decreased
T3420	14656-14658	IN	denotes	in
T3421	14659-14662	DT	denotes	the
T3423	14663-14667	JJ	denotes	wild
T3424	14668-14672	NN	denotes	type
T3422	14673-14677	NNS	denotes	mice
T3425	14678-14681	CC	denotes	but
T3426	14682-14691	VBD	denotes	increased
T3427	14692-14705	RB	denotes	significantly
T3428	14706-14708	IN	denotes	in
T3429	14709-14712	DT	denotes	the
T3431	14713-14718	JJ	denotes	total
T3430	14719-14723	NN	denotes	body
T3432	14724-14727	CC	denotes	and
T3433	14728-14730	IN	denotes	in
T3434	14731-14734	DT	denotes	the
T3436	14735-14742	JJ	denotes	femoral
T3437	14743-14746	CC	denotes	and
T3438	14747-14756	JJ	denotes	vertebral
T3435	14757-14764	NNS	denotes	regions
T3439	14765-14767	IN	denotes	of
T3440	14768-14776	NN	denotes	interest
T3441	14777-14779	IN	denotes	in
T3442	14780-14783	DT	denotes	the
T3443	14784-14789	NN	denotes	Sam68
T3444	14789-14790	SYM	denotes	−
T3445	14790-14791	HYPH	denotes	/
T3446	14791-14792	SYM	denotes	−
T3447	14793-14794	-LRB-	denotes	(
T3448	14794-14799	NN	denotes	Table
T3449	14800-14801	CD	denotes	2
T3450	14801-14802	-RRB-	denotes	)
T3451	14802-14803	.	denotes	.
T3452	14803-14942	sentence	denotes	These data showed that Sam68−/− mice continued to thrive and accrue bone in the axial and appendicular skeleton for longer than 12 months.
T3453	14804-14809	DT	denotes	These
T3454	14810-14814	NNS	denotes	data
T3455	14815-14821	VBD	denotes	showed
T3456	14822-14826	IN	denotes	that
T3458	14827-14832	NN	denotes	Sam68
T3460	14832-14833	SYM	denotes	−
T3461	14833-14834	HYPH	denotes	/
T3462	14834-14835	SYM	denotes	−
T3459	14836-14840	NNS	denotes	mice
T3457	14841-14850	VBD	denotes	continued
T3463	14851-14853	TO	denotes	to
T3464	14854-14860	VB	denotes	thrive
T3465	14861-14864	CC	denotes	and
T3466	14865-14871	VB	denotes	accrue
T3467	14872-14876	NN	denotes	bone
T3468	14877-14879	IN	denotes	in
T3469	14880-14883	DT	denotes	the
T3471	14884-14889	JJ	denotes	axial
T3472	14890-14893	CC	denotes	and
T3473	14894-14906	JJ	denotes	appendicular
T3470	14907-14915	NN	denotes	skeleton
T3474	14916-14919	IN	denotes	for
T3475	14920-14926	JJR	denotes	longer
T3477	14927-14931	IN	denotes	than
T3476	14932-14934	CD	denotes	12
T3478	14935-14941	NNS	denotes	months
T3479	14941-14942	.	denotes	.
T3480	14942-15088	sentence	denotes	This was in contrast to the situation in age-matched, littermate controls in which a significant amount of bone was lost over the same timeframe.
T3481	14943-14947	DT	denotes	This
T3482	14948-14951	VBD	denotes	was
T3483	14952-14954	IN	denotes	in
T3484	14955-14963	NN	denotes	contrast
T3485	14964-14966	IN	denotes	to
T3486	14967-14970	DT	denotes	the
T3487	14971-14980	NN	denotes	situation
T3488	14981-14983	IN	denotes	in
T3489	14984-14987	NN	denotes	age
T3491	14987-14988	HYPH	denotes	-
T3490	14988-14995	VBN	denotes	matched
T3493	14995-14997	,	denotes	,
T3494	14997-15007	NN	denotes	littermate
T3492	15008-15016	NNS	denotes	controls
T3495	15017-15019	IN	denotes	in
T3497	15020-15025	WDT	denotes	which
T3498	15026-15027	DT	denotes	a
T3500	15028-15039	JJ	denotes	significant
T3499	15040-15046	NN	denotes	amount
T3501	15047-15049	IN	denotes	of
T3502	15050-15054	NN	denotes	bone
T3503	15055-15058	VBD	denotes	was
T3496	15059-15063	VBN	denotes	lost
T3504	15064-15068	IN	denotes	over
T3505	15069-15072	DT	denotes	the
T3507	15073-15077	JJ	denotes	same
T3506	15078-15087	NN	denotes	timeframe
T3508	15087-15088	.	denotes	.
T3587	16375-16380	CD	denotes	Three
T3589	16380-16381	HYPH	denotes	-
T3588	16381-16392	JJ	denotes	Dimensional
T3590	16393-16405	NN	denotes	Architecture
T3592	16406-16408	IN	denotes	of
T3593	16409-16413	NN	denotes	Bone
T3594	16414-16416	VBZ	denotes	Is
T3591	16417-16426	VBN	denotes	Preserved
T3595	16427-16429	IN	denotes	in
T3596	16430-16434	VBN	denotes	Aged
T3598	16435-16440	NN	denotes	Sam68
T3599	16440-16441	SYM	denotes	−
T3600	16441-16442	HYPH	denotes	/
T3601	16442-16443	SYM	denotes	−
T3597	16444-16448	NNS	denotes	Mice
T3602	16448-16670	sentence	denotes	Bone loss and compromised architecture are characteristic features of the skeletons of aged C57BL/6 mice and resemble the clinical features of age-related bone loss in humans that can predispose an individual to fracture.
T3603	16449-16453	NN	denotes	Bone
T3604	16454-16458	NN	denotes	loss
T3606	16459-16462	CC	denotes	and
T3607	16463-16474	VBN	denotes	compromised
T3608	16475-16487	NN	denotes	architecture
T3605	16488-16491	VBP	denotes	are
T3609	16492-16506	JJ	denotes	characteristic
T3610	16507-16515	NNS	denotes	features
T3611	16516-16518	IN	denotes	of
T3612	16519-16522	DT	denotes	the
T3613	16523-16532	NNS	denotes	skeletons
T3614	16533-16535	IN	denotes	of
T3615	16536-16540	JJ	denotes	aged
T3617	16541-16546	NN	denotes	C57BL
T3618	16546-16547	HYPH	denotes	/
T3619	16547-16548	CD	denotes	6
T3616	16549-16553	NNS	denotes	mice
T3620	16554-16557	CC	denotes	and
T3621	16558-16566	VBP	denotes	resemble
T3622	16567-16570	DT	denotes	the
T3624	16571-16579	JJ	denotes	clinical
T3623	16580-16588	NNS	denotes	features
T3625	16589-16591	IN	denotes	of
T3626	16592-16595	NN	denotes	age
T3628	16595-16596	HYPH	denotes	-
T3627	16596-16603	VBN	denotes	related
T3630	16604-16608	NN	denotes	bone
T3629	16609-16613	NN	denotes	loss
T3631	16614-16616	IN	denotes	in
T3632	16617-16623	NNS	denotes	humans
T3633	16624-16628	WDT	denotes	that
T3635	16629-16632	MD	denotes	can
T3634	16633-16643	VB	denotes	predispose
T3636	16644-16646	DT	denotes	an
T3637	16647-16657	NN	denotes	individual
T3638	16658-16660	IN	denotes	to
T3639	16661-16669	NN	denotes	fracture
T3640	16669-16670	.	denotes	.
T3641	16670-16976	sentence	denotes	Quantification of the micro-CT data shown in Figure 3B confirmed the reduction in bone volume compared with tissue volume (BV/TV; Figure 4A, left) in the 12-month-old Sam68+/+ mice (hatched bars) compared with 4-month-old mice of both genotypes (solid bars) and 12-month-old Sam68−/− mice (stippled bars).
T3642	16671-16685	NN	denotes	Quantification
T3644	16686-16688	IN	denotes	of
T3645	16689-16692	DT	denotes	the
T3647	16693-16701	NN	denotes	micro-CT
T3646	16702-16706	NNS	denotes	data
T3648	16707-16712	VBN	denotes	shown
T3649	16713-16715	IN	denotes	in
T3650	16716-16722	NN	denotes	Figure
T3651	16723-16725	NN	denotes	3B
T3643	16726-16735	VBD	denotes	confirmed
T3652	16736-16739	DT	denotes	the
T3653	16740-16749	NN	denotes	reduction
T3654	16750-16752	IN	denotes	in
T3655	16753-16757	NN	denotes	bone
T3656	16758-16764	NN	denotes	volume
T3657	16765-16773	VBN	denotes	compared
T3658	16774-16778	IN	denotes	with
T3659	16779-16785	NN	denotes	tissue
T3660	16786-16792	NN	denotes	volume
T3661	16793-16794	-LRB-	denotes	(
T3663	16794-16796	NN	denotes	BV
T3665	16796-16797	HYPH	denotes	/
T3664	16797-16799	NN	denotes	TV
T3666	16799-16800	:	denotes	;
T3667	16801-16807	NN	denotes	Figure
T3662	16808-16810	NN	denotes	4A
T3668	16810-16812	,	denotes	,
T3669	16812-16816	JJ	denotes	left
T3670	16816-16817	-RRB-	denotes	)
T3671	16818-16820	IN	denotes	in
T3672	16821-16824	DT	denotes	the
T3674	16825-16827	CD	denotes	12
T3676	16827-16828	HYPH	denotes	-
T3675	16828-16833	NN	denotes	month
T3678	16833-16834	HYPH	denotes	-
T3677	16834-16837	JJ	denotes	old
T3679	16838-16843	NN	denotes	Sam68
T3680	16843-16844	SYM	denotes	+
T3681	16844-16845	HYPH	denotes	/
T3682	16845-16846	SYM	denotes	+
T3673	16847-16851	NNS	denotes	mice
T3683	16852-16853	-LRB-	denotes	(
T3685	16853-16860	VBN	denotes	hatched
T3684	16861-16865	NNS	denotes	bars
T3686	16865-16866	-RRB-	denotes	)
T3687	16867-16875	VBN	denotes	compared
T3688	16876-16880	IN	denotes	with
T3689	16881-16882	CD	denotes	4
T3691	16882-16883	HYPH	denotes	-
T3690	16883-16888	NN	denotes	month
T3693	16888-16889	HYPH	denotes	-
T3692	16889-16892	JJ	denotes	old
T3694	16893-16897	NNS	denotes	mice
T3695	16898-16900	IN	denotes	of
T3696	16901-16905	DT	denotes	both
T3697	16906-16915	NNS	denotes	genotypes
T3698	16916-16917	-LRB-	denotes	(
T3700	16917-16922	JJ	denotes	solid
T3699	16923-16927	NNS	denotes	bars
T3701	16927-16928	-RRB-	denotes	)
T3702	16929-16932	CC	denotes	and
T3703	16933-16935	CD	denotes	12
T3705	16935-16936	HYPH	denotes	-
T3704	16936-16941	NN	denotes	month
T3707	16941-16942	HYPH	denotes	-
T3706	16942-16945	JJ	denotes	old
T3709	16946-16951	NN	denotes	Sam68
T3710	16951-16952	SYM	denotes	−
T3711	16952-16953	HYPH	denotes	/
T3712	16953-16954	SYM	denotes	−
T3708	16955-16959	NNS	denotes	mice
T3713	16960-16961	-LRB-	denotes	(
T3715	16961-16969	VBN	denotes	stippled
T3714	16970-16974	NNS	denotes	bars
T3716	16974-16975	-RRB-	denotes	)
T3717	16975-16976	.	denotes	.
T3718	16976-17173	sentence	denotes	This was associated with a significant increase (p < 0.01; denoted by the asterisks) in the structure model index, which measures the ratio of plate-like to rod-like structures (Figure 4A, right).
T3719	16977-16981	DT	denotes	This
T3721	16982-16985	VBD	denotes	was
T3720	16986-16996	VBN	denotes	associated
T3722	16997-17001	IN	denotes	with
T3723	17002-17003	DT	denotes	a
T3725	17004-17015	JJ	denotes	significant
T3724	17016-17024	NN	denotes	increase
T3726	17025-17026	-LRB-	denotes	(
T3728	17026-17027	NN	denotes	p
T3730	17028-17029	SYM	denotes	<
T3729	17030-17034	CD	denotes	0.01
T3731	17034-17035	:	denotes	;
T3727	17036-17043	VBN	denotes	denoted
T3732	17044-17046	IN	denotes	by
T3733	17047-17050	DT	denotes	the
T3734	17051-17060	NNS	denotes	asterisks
T3735	17060-17061	-RRB-	denotes	)
T3736	17062-17064	IN	denotes	in
T3737	17065-17068	DT	denotes	the
T3739	17069-17078	NN	denotes	structure
T3740	17079-17084	NN	denotes	model
T3738	17085-17090	NN	denotes	index
T3741	17090-17092	,	denotes	,
T3742	17092-17097	WDT	denotes	which
T3743	17098-17106	VBZ	denotes	measures
T3744	17107-17110	DT	denotes	the
T3745	17111-17116	NN	denotes	ratio
T3746	17117-17119	IN	denotes	of
T3747	17120-17125	NN	denotes	plate
T3749	17125-17126	HYPH	denotes	-
T3748	17126-17130	JJ	denotes	like
T3750	17131-17133	IN	denotes	to
T3751	17134-17137	NN	denotes	rod
T3753	17137-17138	HYPH	denotes	-
T3752	17138-17142	JJ	denotes	like
T3754	17143-17153	NNS	denotes	structures
T3755	17154-17155	-LRB-	denotes	(
T3757	17155-17161	NN	denotes	Figure
T3756	17162-17164	NN	denotes	4A
T3758	17164-17166	,	denotes	,
T3759	17166-17171	NN	denotes	right
T3760	17171-17172	-RRB-	denotes	)
T3761	17172-17173	.	denotes	.
T3762	17173-17408	sentence	denotes	A quantifiable increase in the mean trabecular separation (Tb.Sp) (unpublished data) in 12-month-old Sam68+/+ mice was due to an increase in the percentage of spaces falling in the range of 350 to 700 μm (Figure 4B, red hatched line).
T3763	17174-17175	DT	denotes	A
T3765	17176-17188	JJ	denotes	quantifiable
T3764	17189-17197	NN	denotes	increase
T3767	17198-17200	IN	denotes	in
T3768	17201-17204	DT	denotes	the
T3770	17205-17209	NN	denotes	mean
T3771	17210-17220	JJ	denotes	trabecular
T3769	17221-17231	NN	denotes	separation
T3772	17232-17233	-LRB-	denotes	(
T3773	17233-17238	NN	denotes	Tb.Sp
T3774	17238-17239	-RRB-	denotes	)
T3775	17240-17241	-LRB-	denotes	(
T3777	17241-17252	JJ	denotes	unpublished
T3776	17253-17257	NNS	denotes	data
T3778	17257-17258	-RRB-	denotes	)
T3779	17259-17261	IN	denotes	in
T3780	17262-17264	CD	denotes	12
T3782	17264-17265	HYPH	denotes	-
T3781	17265-17270	NN	denotes	month
T3784	17270-17271	HYPH	denotes	-
T3783	17271-17274	JJ	denotes	old
T3786	17275-17280	NN	denotes	Sam68
T3787	17280-17281	SYM	denotes	+
T3788	17281-17282	HYPH	denotes	/
T3789	17282-17283	SYM	denotes	+
T3785	17284-17288	NNS	denotes	mice
T3766	17289-17292	VBD	denotes	was
T3790	17293-17296	IN	denotes	due
T3791	17297-17299	IN	denotes	to
T3792	17300-17302	DT	denotes	an
T3793	17303-17311	NN	denotes	increase
T3794	17312-17314	IN	denotes	in
T3795	17315-17318	DT	denotes	the
T3796	17319-17329	NN	denotes	percentage
T3797	17330-17332	IN	denotes	of
T3798	17333-17339	NNS	denotes	spaces
T3799	17340-17347	VBG	denotes	falling
T3800	17348-17350	IN	denotes	in
T3801	17351-17354	DT	denotes	the
T3802	17355-17360	NN	denotes	range
T3803	17361-17363	IN	denotes	of
T3804	17364-17367	CD	denotes	350
T3806	17368-17370	IN	denotes	to
T3805	17371-17374	CD	denotes	700
T3807	17375-17377	NN	denotes	μm
T3808	17378-17379	-LRB-	denotes	(
T3810	17379-17385	NN	denotes	Figure
T3809	17386-17388	NN	denotes	4B
T3811	17388-17390	,	denotes	,
T3812	17390-17393	JJ	denotes	red
T3814	17394-17401	VBN	denotes	hatched
T3813	17402-17406	NN	denotes	line
T3815	17406-17407	-RRB-	denotes	)
T3816	17407-17408	.	denotes	.
T3817	17408-17502	sentence	denotes	Trabecular thickness remained constant among the different groups of mice (unpublished data).
T3818	17409-17419	JJ	denotes	Trabecular
T3819	17420-17429	NN	denotes	thickness
T3820	17430-17438	VBD	denotes	remained
T3821	17439-17447	JJ	denotes	constant
T3822	17448-17453	IN	denotes	among
T3823	17454-17457	DT	denotes	the
T3825	17458-17467	JJ	denotes	different
T3824	17468-17474	NNS	denotes	groups
T3826	17475-17477	IN	denotes	of
T3827	17478-17482	NNS	denotes	mice
T3828	17483-17484	-LRB-	denotes	(
T3830	17484-17495	JJ	denotes	unpublished
T3829	17496-17500	NNS	denotes	data
T3831	17500-17501	-RRB-	denotes	)
T3832	17501-17502	.	denotes	.
T3833	17502-17690	sentence	denotes	In effect, this meant that there were fewer trabeculae, rather than equivalent numbers of thin trabeculae, in the 12-month-old Sam68+/+ mice compared with any of the other groups of mice.
T3834	17503-17505	IN	denotes	In
T3836	17506-17512	NN	denotes	effect
T3837	17512-17514	,	denotes	,
T3838	17514-17518	DT	denotes	this
T3835	17519-17524	VBD	denotes	meant
T3839	17525-17529	IN	denotes	that
T3841	17530-17535	EX	denotes	there
T3840	17536-17540	VBD	denotes	were
T3842	17541-17546	JJR	denotes	fewer
T3843	17547-17557	NNS	denotes	trabeculae
T3844	17557-17559	,	denotes	,
T3845	17559-17565	RB	denotes	rather
T3846	17566-17570	IN	denotes	than
T3847	17571-17581	JJ	denotes	equivalent
T3848	17582-17589	NNS	denotes	numbers
T3849	17590-17592	IN	denotes	of
T3850	17593-17597	JJ	denotes	thin
T3851	17598-17608	NNS	denotes	trabeculae
T3852	17608-17610	,	denotes	,
T3853	17610-17612	IN	denotes	in
T3854	17613-17616	DT	denotes	the
T3856	17617-17619	CD	denotes	12
T3858	17619-17620	HYPH	denotes	-
T3857	17620-17625	NN	denotes	month
T3860	17625-17626	HYPH	denotes	-
T3859	17626-17629	JJ	denotes	old
T3861	17630-17635	NN	denotes	Sam68
T3862	17635-17636	SYM	denotes	+
T3863	17636-17637	HYPH	denotes	/
T3864	17637-17638	SYM	denotes	+
T3855	17639-17643	NNS	denotes	mice
T3865	17644-17652	VBN	denotes	compared
T3866	17653-17657	IN	denotes	with
T3867	17658-17661	DT	denotes	any
T3868	17662-17664	IN	denotes	of
T3869	17665-17668	DT	denotes	the
T3871	17669-17674	JJ	denotes	other
T3870	17675-17681	NNS	denotes	groups
T3872	17682-17684	IN	denotes	of
T3873	17685-17689	NNS	denotes	mice
T3874	17689-17690	.	denotes	.
T4163	18580-18584	NN	denotes	Bone
T4164	18585-18595	NN	denotes	Remodeling
T4166	18596-18598	VBZ	denotes	Is
T4165	18599-18608	VBN	denotes	Preserved
T4167	18609-18611	IN	denotes	in
T4168	18612-18616	VBN	denotes	Aged
T4170	18617-18622	NN	denotes	Sam68
T4171	18622-18623	SYM	denotes	−
T4172	18623-18624	HYPH	denotes	/
T4173	18624-18625	SYM	denotes	−
T4169	18626-18630	NNS	denotes	Mice
T4174	18630-18847	sentence	denotes	To further define the mechanisms involved in the preservation of bone mass in aged Sam68−/− mice, we prepared sections from plastic-embedded femora and tibia to evaluate osteoblast and osteoclast activity (Figure 5).
T4175	18631-18633	TO	denotes	To
T4177	18634-18641	RB	denotes	further
T4176	18642-18648	VB	denotes	define
T4179	18649-18652	DT	denotes	the
T4180	18653-18663	NNS	denotes	mechanisms
T4181	18664-18672	VBN	denotes	involved
T4182	18673-18675	IN	denotes	in
T4183	18676-18679	DT	denotes	the
T4184	18680-18692	NN	denotes	preservation
T4185	18693-18695	IN	denotes	of
T4186	18696-18700	NN	denotes	bone
T4187	18701-18705	NN	denotes	mass
T4188	18706-18708	IN	denotes	in
T4189	18709-18713	JJ	denotes	aged
T4191	18714-18719	NN	denotes	Sam68
T4192	18719-18720	SYM	denotes	−
T4193	18720-18721	HYPH	denotes	/
T4194	18721-18722	SYM	denotes	−
T4190	18723-18727	NNS	denotes	mice
T4195	18727-18729	,	denotes	,
T4196	18729-18731	PRP	denotes	we
T4178	18732-18740	VBD	denotes	prepared
T4197	18741-18749	NNS	denotes	sections
T4198	18750-18754	IN	denotes	from
T4199	18755-18762	NN	denotes	plastic
T4201	18762-18763	HYPH	denotes	-
T4200	18763-18771	VBN	denotes	embedded
T4202	18772-18778	NNS	denotes	femora
T4203	18779-18782	CC	denotes	and
T4204	18783-18788	NN	denotes	tibia
T4205	18789-18791	TO	denotes	to
T4206	18792-18800	VB	denotes	evaluate
T4207	18801-18811	NN	denotes	osteoblast
T4209	18812-18815	CC	denotes	and
T4210	18816-18826	NN	denotes	osteoclast
T4208	18827-18835	NN	denotes	activity
T4211	18836-18837	-LRB-	denotes	(
T4212	18837-18843	NN	denotes	Figure
T4213	18844-18845	CD	denotes	5
T4214	18845-18846	-RRB-	denotes	)
T4215	18846-18847	.	denotes	.
T4216	18847-19099	sentence	denotes	In situ enzyme histochemical staining for alkaline phosphatase (ALP; brown stain) activity was used as a biomarker for osteoblasts and tartrate-resistant ALP (tartrate-resistant acid phosphatase [TRAP]; red stain) activity as a marker for osteoclasts.
T4217	18848-18850	FW	denotes	In
T4218	18851-18855	FW	denotes	situ
T4220	18856-18862	NN	denotes	enzyme
T4221	18863-18876	JJ	denotes	histochemical
T4219	18877-18885	NN	denotes	staining
T4223	18886-18889	IN	denotes	for
T4224	18890-18898	NN	denotes	alkaline
T4225	18899-18910	NN	denotes	phosphatase
T4227	18911-18912	-LRB-	denotes	(
T4228	18912-18915	NN	denotes	ALP
T4229	18915-18916	:	denotes	;
T4231	18917-18922	JJ	denotes	brown
T4230	18923-18928	NN	denotes	stain
T4232	18928-18929	-RRB-	denotes	)
T4226	18930-18938	NN	denotes	activity
T4233	18939-18942	VBD	denotes	was
T4222	18943-18947	VBN	denotes	used
T4234	18948-18950	IN	denotes	as
T4235	18951-18952	DT	denotes	a
T4236	18953-18962	NN	denotes	biomarker
T4237	18963-18966	IN	denotes	for
T4238	18967-18978	NNS	denotes	osteoblasts
T4239	18979-18982	CC	denotes	and
T4240	18983-18991	NN	denotes	tartrate
T4242	18991-18992	HYPH	denotes	-
T4241	18992-19001	JJ	denotes	resistant
T4244	19002-19005	NN	denotes	ALP
T4245	19006-19007	-LRB-	denotes	(
T4247	19007-19015	NN	denotes	tartrate
T4249	19015-19016	HYPH	denotes	-
T4248	19016-19025	JJ	denotes	resistant
T4251	19026-19030	NN	denotes	acid
T4250	19031-19042	NN	denotes	phosphatase
T4252	19043-19044	-LRB-	denotes	[
T4253	19044-19048	NN	denotes	TRAP
T4254	19048-19049	-RRB-	denotes	]
T4255	19049-19050	:	denotes	;
T4256	19051-19054	JJ	denotes	red
T4246	19055-19060	NN	denotes	stain
T4257	19060-19061	-RRB-	denotes	)
T4243	19062-19070	NN	denotes	activity
T4258	19071-19073	IN	denotes	as
T4259	19074-19075	DT	denotes	a
T4260	19076-19082	NN	denotes	marker
T4261	19083-19086	IN	denotes	for
T4262	19087-19098	NNS	denotes	osteoclasts
T4263	19098-19099	.	denotes	.
T4264	19099-19219	sentence	denotes	Little difference was seen between Sam68+/+ (Figure 5A and 5B) and Sam68−/− (Figure 5C and 5D) mice at 4 months of age.
T4265	19100-19106	JJ	denotes	Little
T4266	19107-19117	NN	denotes	difference
T4268	19118-19121	VBD	denotes	was
T4267	19122-19126	VBN	denotes	seen
T4269	19127-19134	IN	denotes	between
T4270	19135-19140	NN	denotes	Sam68
T4272	19140-19141	SYM	denotes	+
T4273	19141-19142	HYPH	denotes	/
T4274	19142-19143	SYM	denotes	+
T4275	19144-19145	-LRB-	denotes	(
T4277	19145-19151	NN	denotes	Figure
T4276	19152-19154	NN	denotes	5A
T4278	19155-19158	CC	denotes	and
T4279	19159-19161	NN	denotes	5B
T4280	19161-19162	-RRB-	denotes	)
T4281	19163-19166	CC	denotes	and
T4282	19167-19172	NN	denotes	Sam68
T4283	19172-19173	SYM	denotes	−
T4284	19173-19174	HYPH	denotes	/
T4285	19174-19175	SYM	denotes	−
T4286	19176-19177	-LRB-	denotes	(
T4288	19177-19183	NN	denotes	Figure
T4287	19184-19186	NN	denotes	5C
T4289	19187-19190	CC	denotes	and
T4290	19191-19193	NN	denotes	5D
T4291	19193-19194	-RRB-	denotes	)
T4271	19195-19199	NNS	denotes	mice
T4292	19200-19202	IN	denotes	at
T4293	19203-19204	CD	denotes	4
T4294	19205-19211	NNS	denotes	months
T4295	19212-19214	IN	denotes	of
T4296	19215-19218	NN	denotes	age
T4297	19218-19219	.	denotes	.
T4298	19219-19387	sentence	denotes	ALP- and TRAP-positive cells were reduced in the 12-month-old Sam68+/+ mice (Figure 5E and 5F) and remained unchanged in 12-month-old Sam68−/− mice (Figure 5G and 5H).
T4299	19220-19223	NN	denotes	ALP
T4301	19223-19224	HYPH	denotes	-
T4302	19225-19228	CC	denotes	and
T4303	19229-19233	NN	denotes	TRAP
T4304	19233-19234	HYPH	denotes	-
T4300	19234-19242	JJ	denotes	positive
T4305	19243-19248	NNS	denotes	cells
T4307	19249-19253	VBD	denotes	were
T4306	19254-19261	VBN	denotes	reduced
T4308	19262-19264	IN	denotes	in
T4309	19265-19268	DT	denotes	the
T4311	19269-19271	CD	denotes	12
T4313	19271-19272	HYPH	denotes	-
T4312	19272-19277	NN	denotes	month
T4315	19277-19278	HYPH	denotes	-
T4314	19278-19281	JJ	denotes	old
T4316	19282-19287	NN	denotes	Sam68
T4317	19287-19288	SYM	denotes	+
T4318	19288-19289	HYPH	denotes	/
T4319	19289-19290	SYM	denotes	+
T4310	19291-19295	NNS	denotes	mice
T4320	19296-19297	-LRB-	denotes	(
T4322	19297-19303	NN	denotes	Figure
T4321	19304-19306	NN	denotes	5E
T4323	19307-19310	CC	denotes	and
T4324	19311-19313	NN	denotes	5F
T4325	19313-19314	-RRB-	denotes	)
T4326	19315-19318	CC	denotes	and
T4327	19319-19327	VBD	denotes	remained
T4328	19328-19337	JJ	denotes	unchanged
T4329	19338-19340	IN	denotes	in
T4330	19341-19343	CD	denotes	12
T4332	19343-19344	HYPH	denotes	-
T4331	19344-19349	NN	denotes	month
T4334	19349-19350	HYPH	denotes	-
T4333	19350-19353	JJ	denotes	old
T4336	19354-19359	NN	denotes	Sam68
T4337	19359-19360	SYM	denotes	−
T4338	19360-19361	HYPH	denotes	/
T4339	19361-19362	SYM	denotes	−
T4335	19363-19367	NNS	denotes	mice
T4340	19368-19369	-LRB-	denotes	(
T4342	19369-19375	NN	denotes	Figure
T4341	19376-19378	NN	denotes	5G
T4343	19379-19382	CC	denotes	and
T4344	19383-19385	NN	denotes	5H
T4345	19385-19386	-RRB-	denotes	)
T4346	19386-19387	.	denotes	.
T4347	19387-19625	sentence	denotes	The reduction in both osteoblast and osteoclast activity in the 12-month-old Sam68+/+ mice argued against bone being lost primarily due to a relative increase in osteoclast over osteoblast activity, as seen in high turnover disease [49].
T4348	19388-19391	DT	denotes	The
T4349	19392-19401	NN	denotes	reduction
T4351	19402-19404	IN	denotes	in
T4352	19405-19409	CC	denotes	both
T4353	19410-19420	NN	denotes	osteoblast
T4355	19421-19424	CC	denotes	and
T4356	19425-19435	NN	denotes	osteoclast
T4354	19436-19444	NN	denotes	activity
T4357	19445-19447	IN	denotes	in
T4358	19448-19451	DT	denotes	the
T4360	19452-19454	CD	denotes	12
T4362	19454-19455	HYPH	denotes	-
T4361	19455-19460	NN	denotes	month
T4364	19460-19461	HYPH	denotes	-
T4363	19461-19464	JJ	denotes	old
T4365	19465-19470	NN	denotes	Sam68
T4366	19470-19471	SYM	denotes	+
T4367	19471-19472	HYPH	denotes	/
T4368	19472-19473	SYM	denotes	+
T4359	19474-19478	NNS	denotes	mice
T4350	19479-19485	VBD	denotes	argued
T4369	19486-19493	IN	denotes	against
T4370	19494-19498	NN	denotes	bone
T4372	19499-19504	VBG	denotes	being
T4371	19505-19509	VBN	denotes	lost
T4373	19510-19519	RB	denotes	primarily
T4374	19520-19523	IN	denotes	due
T4375	19524-19526	IN	denotes	to
T4376	19527-19528	DT	denotes	a
T4378	19529-19537	JJ	denotes	relative
T4377	19538-19546	NN	denotes	increase
T4379	19547-19549	IN	denotes	in
T4380	19550-19560	NN	denotes	osteoclast
T4381	19561-19565	IN	denotes	over
T4382	19566-19576	NN	denotes	osteoblast
T4383	19577-19585	NN	denotes	activity
T4384	19585-19587	,	denotes	,
T4385	19587-19589	IN	denotes	as
T4386	19590-19594	VBN	denotes	seen
T4387	19595-19597	IN	denotes	in
T4388	19598-19602	JJ	denotes	high
T4389	19603-19611	NN	denotes	turnover
T4390	19612-19619	NN	denotes	disease
T4391	19620-19621	-LRB-	denotes	[
T4392	19621-19623	CD	denotes	49
T4393	19623-19624	-RRB-	denotes	]
T4394	19624-19625	.	denotes	.
T4395	19625-20379	sentence	denotes	Figure 5 Histologic Analysis of Undecalcified Bone from Sam68+/+ and Sam68−/− Mice Sections of tibia fixed in 4% paraformaldehyde and embedded in plastic were stained for ALP (A–C, E–G) activity to identify osteoblasts or for TRAP (B–D, F–H) activity to identify osteoclasts. Staining patterns were similar in 4-month-old Sam68+/+ (A and B), 4-month-old Sam68−/− (C and D), and 12-month-old Sam68−/− (G and H) mice compared with 12-month-old Sam68+/+ mice (E and F). Magnification at source, left panels ×10 and right panels ×40. Micrographs are representative of those taken from five to seven sections in each group of animals. Histomorphometric analyses [50] of the long bones (Table 3) corroborated the radiologic evidence of age-related bone loss.
T4396	20257-20274	JJ	denotes	Histomorphometric
T4397	20275-20283	NNS	denotes	analyses
T4399	20284-20285	-LRB-	denotes	[
T4400	20285-20287	CD	denotes	50
T4401	20287-20288	-RRB-	denotes	]
T4402	20289-20291	IN	denotes	of
T4403	20292-20295	DT	denotes	the
T4405	20296-20300	JJ	denotes	long
T4404	20301-20306	NNS	denotes	bones
T4406	20307-20308	-LRB-	denotes	(
T4407	20308-20313	NN	denotes	Table
T4408	20314-20315	CD	denotes	3
T4409	20315-20316	-RRB-	denotes	)
T4398	20317-20329	VBD	denotes	corroborated
T4410	20330-20333	DT	denotes	the
T4412	20334-20344	JJ	denotes	radiologic
T4411	20345-20353	NN	denotes	evidence
T4413	20354-20356	IN	denotes	of
T4414	20357-20360	NN	denotes	age
T4416	20360-20361	HYPH	denotes	-
T4415	20361-20368	VBN	denotes	related
T4418	20369-20373	NN	denotes	bone
T4417	20374-20378	NN	denotes	loss
T4419	20378-20379	.	denotes	.
T4420	20379-20566	sentence	denotes	Bone volume (BV/TV), newly formed osteoid (OV/TV), and mineral apposition rate (MAR) were all significantly reduced in 12-month-old Sam68+/+ mice compared with 4-month-old Sam68+/+ mice.
T4421	20380-20384	NN	denotes	Bone
T4422	20385-20391	NN	denotes	volume
T4424	20392-20393	-LRB-	denotes	(
T4425	20393-20395	NN	denotes	BV
T4427	20395-20396	HYPH	denotes	/
T4426	20396-20398	NN	denotes	TV
T4428	20398-20399	-RRB-	denotes	)
T4429	20399-20401	,	denotes	,
T4430	20401-20406	RB	denotes	newly
T4431	20407-20413	VBN	denotes	formed
T4432	20414-20421	JJ	denotes	osteoid
T4433	20422-20423	-LRB-	denotes	(
T4434	20423-20425	NN	denotes	OV
T4436	20425-20426	HYPH	denotes	/
T4435	20426-20428	NN	denotes	TV
T4437	20428-20429	-RRB-	denotes	)
T4438	20429-20431	,	denotes	,
T4439	20431-20434	CC	denotes	and
T4440	20435-20442	NN	denotes	mineral
T4442	20443-20453	NN	denotes	apposition
T4441	20454-20458	NN	denotes	rate
T4443	20459-20460	-LRB-	denotes	(
T4444	20460-20463	NN	denotes	MAR
T4445	20463-20464	-RRB-	denotes	)
T4446	20465-20469	VBD	denotes	were
T4447	20470-20473	DT	denotes	all
T4448	20474-20487	RB	denotes	significantly
T4423	20488-20495	VBN	denotes	reduced
T4449	20496-20498	IN	denotes	in
T4450	20499-20501	CD	denotes	12
T4452	20501-20502	HYPH	denotes	-
T4451	20502-20507	NN	denotes	month
T4454	20507-20508	HYPH	denotes	-
T4453	20508-20511	JJ	denotes	old
T4456	20512-20517	NN	denotes	Sam68
T4457	20517-20518	SYM	denotes	+
T4458	20518-20519	HYPH	denotes	/
T4459	20519-20520	SYM	denotes	+
T4455	20521-20525	NNS	denotes	mice
T4460	20526-20534	VBN	denotes	compared
T4461	20535-20539	IN	denotes	with
T4462	20540-20541	CD	denotes	4
T4464	20541-20542	HYPH	denotes	-
T4463	20542-20547	NN	denotes	month
T4466	20547-20548	HYPH	denotes	-
T4465	20548-20551	JJ	denotes	old
T4468	20552-20557	NN	denotes	Sam68
T4469	20557-20558	SYM	denotes	+
T4470	20558-20559	HYPH	denotes	/
T4471	20559-20560	SYM	denotes	+
T4467	20561-20565	NNS	denotes	mice
T4472	20565-20566	.	denotes	.
T4473	20566-20749	sentence	denotes	These reductions were associated with a significant increase in marrow fat (FV/TV) and decreased numbers of osteoblasts (nOB/TV) and osteoclasts (nOC/TV) per tissue volume (Table 3).
T4474	20567-20572	DT	denotes	These
T4475	20573-20583	NNS	denotes	reductions
T4477	20584-20588	VBD	denotes	were
T4476	20589-20599	VBN	denotes	associated
T4478	20600-20604	IN	denotes	with
T4479	20605-20606	DT	denotes	a
T4481	20607-20618	JJ	denotes	significant
T4480	20619-20627	NN	denotes	increase
T4482	20628-20630	IN	denotes	in
T4483	20631-20637	NN	denotes	marrow
T4484	20638-20641	NN	denotes	fat
T4485	20642-20643	-LRB-	denotes	(
T4487	20643-20645	NN	denotes	FV
T4488	20645-20646	HYPH	denotes	/
T4486	20646-20648	NN	denotes	TV
T4489	20648-20649	-RRB-	denotes	)
T4490	20650-20653	CC	denotes	and
T4491	20654-20663	VBN	denotes	decreased
T4492	20664-20671	NNS	denotes	numbers
T4493	20672-20674	IN	denotes	of
T4494	20675-20686	NNS	denotes	osteoblasts
T4495	20687-20688	-LRB-	denotes	(
T4497	20688-20691	NN	denotes	nOB
T4498	20691-20692	HYPH	denotes	/
T4496	20692-20694	NN	denotes	TV
T4499	20694-20695	-RRB-	denotes	)
T4500	20696-20699	CC	denotes	and
T4501	20700-20711	NNS	denotes	osteoclasts
T4502	20712-20713	-LRB-	denotes	(
T4504	20713-20716	NN	denotes	nOC
T4505	20716-20717	HYPH	denotes	/
T4503	20717-20719	NN	denotes	TV
T4506	20719-20720	-RRB-	denotes	)
T4507	20721-20724	IN	denotes	per
T4508	20725-20731	NN	denotes	tissue
T4509	20732-20738	NN	denotes	volume
T4510	20739-20740	-LRB-	denotes	(
T4511	20740-20745	NN	denotes	Table
T4512	20746-20747	CD	denotes	3
T4513	20747-20748	-RRB-	denotes	)
T4514	20748-20749	.	denotes	.
T4515	20749-20948	sentence	denotes	These results were in contrast to those of the 12-month-old Sam68−/− mice in which all histomorphometric parameters, including marrow fat, resembled those of young mice of either genotype (Table 3).
T4516	20750-20755	DT	denotes	These
T4517	20756-20763	NNS	denotes	results
T4518	20764-20768	VBD	denotes	were
T4519	20769-20771	IN	denotes	in
T4520	20772-20780	NN	denotes	contrast
T4521	20781-20783	IN	denotes	to
T4522	20784-20789	DT	denotes	those
T4523	20790-20792	IN	denotes	of
T4524	20793-20796	DT	denotes	the
T4526	20797-20799	CD	denotes	12
T4528	20799-20800	HYPH	denotes	-
T4527	20800-20805	NN	denotes	month
T4530	20805-20806	HYPH	denotes	-
T4529	20806-20809	JJ	denotes	old
T4531	20810-20815	NN	denotes	Sam68
T4532	20815-20816	SYM	denotes	−
T4533	20816-20817	HYPH	denotes	/
T4534	20817-20818	SYM	denotes	−
T4525	20819-20823	NNS	denotes	mice
T4535	20824-20826	IN	denotes	in
T4537	20827-20832	WDT	denotes	which
T4538	20833-20836	DT	denotes	all
T4540	20837-20854	JJ	denotes	histomorphometric
T4539	20855-20865	NNS	denotes	parameters
T4541	20865-20867	,	denotes	,
T4542	20867-20876	VBG	denotes	including
T4543	20877-20883	NN	denotes	marrow
T4544	20884-20887	NN	denotes	fat
T4545	20887-20889	,	denotes	,
T4536	20889-20898	VBD	denotes	resembled
T4546	20899-20904	DT	denotes	those
T4547	20905-20907	IN	denotes	of
T4548	20908-20913	JJ	denotes	young
T4549	20914-20918	NNS	denotes	mice
T4550	20919-20921	IN	denotes	of
T4551	20922-20928	CC	denotes	either
T4552	20929-20937	NN	denotes	genotype
T4553	20938-20939	-LRB-	denotes	(
T4554	20939-20944	NN	denotes	Table
T4555	20945-20946	CD	denotes	3
T4556	20946-20947	-RRB-	denotes	)
T4557	20947-20948	.	denotes	.
T4558	20948-21155	sentence	denotes	When cells were expressed as a function of the bone perimeter (nOB/BP, nOC/BP), there were no statistical differences and the ratio of osteoblasts to osteoclasts was similar in all groups of mice (Table 3).
T4559	20949-20953	WRB	denotes	When
T4561	20954-20959	NNS	denotes	cells
T4562	20960-20964	VBD	denotes	were
T4560	20965-20974	VBN	denotes	expressed
T4564	20975-20977	IN	denotes	as
T4565	20978-20979	DT	denotes	a
T4566	20980-20988	NN	denotes	function
T4567	20989-20991	IN	denotes	of
T4568	20992-20995	DT	denotes	the
T4570	20996-21000	NN	denotes	bone
T4569	21001-21010	NN	denotes	perimeter
T4571	21011-21012	-LRB-	denotes	(
T4573	21012-21015	NN	denotes	nOB
T4574	21015-21016	HYPH	denotes	/
T4572	21016-21018	NN	denotes	BP
T4575	21018-21020	,	denotes	,
T4576	21020-21023	NN	denotes	nOC
T4578	21023-21024	HYPH	denotes	/
T4577	21024-21026	NN	denotes	BP
T4579	21026-21027	-RRB-	denotes	)
T4580	21027-21029	,	denotes	,
T4581	21029-21034	EX	denotes	there
T4563	21035-21039	VBD	denotes	were
T4582	21040-21042	DT	denotes	no
T4584	21043-21054	JJ	denotes	statistical
T4583	21055-21066	NNS	denotes	differences
T4585	21067-21070	CC	denotes	and
T4586	21071-21074	DT	denotes	the
T4587	21075-21080	NN	denotes	ratio
T4589	21081-21083	IN	denotes	of
T4590	21084-21095	NNS	denotes	osteoblasts
T4591	21096-21098	IN	denotes	to
T4592	21099-21110	NNS	denotes	osteoclasts
T4588	21111-21114	VBD	denotes	was
T4593	21115-21122	JJ	denotes	similar
T4594	21123-21125	IN	denotes	in
T4595	21126-21129	DT	denotes	all
T4596	21130-21136	NNS	denotes	groups
T4597	21137-21139	IN	denotes	of
T4598	21140-21144	NNS	denotes	mice
T4599	21145-21146	-LRB-	denotes	(
T4600	21146-21151	NN	denotes	Table
T4601	21152-21153	CD	denotes	3
T4602	21153-21154	-RRB-	denotes	)
T4603	21154-21155	.	denotes	.
T4604	21155-21449	sentence	denotes	Table 3 Histomorphometric Analysis of Long Bones from 4- and 12-Month-Old Mice Circulating levels of serum C-telopeptide (sCTX; Roche Diagnostics, Mannheim, Germany) and ALP showed little difference among the groups, except for a small decrease in 12-month-old Sam68−/− mice and (Dataset S3).
T4605	21236-21247	VBG	denotes	Circulating
T4606	21248-21254	NNS	denotes	levels
T4608	21255-21257	IN	denotes	of
T4609	21258-21263	NN	denotes	serum
T4611	21264-21265	NN	denotes	C
T4612	21265-21266	HYPH	denotes	-
T4610	21266-21277	NN	denotes	telopeptide
T4613	21278-21279	-LRB-	denotes	(
T4615	21279-21283	NN	denotes	sCTX
T4616	21283-21284	:	denotes	;
T4617	21285-21290	NNP	denotes	Roche
T4614	21291-21302	NNP	denotes	Diagnostics
T4618	21302-21304	,	denotes	,
T4619	21304-21312	NNP	denotes	Mannheim
T4620	21312-21314	,	denotes	,
T4621	21314-21321	NNP	denotes	Germany
T4622	21321-21322	-RRB-	denotes	)
T4623	21323-21326	CC	denotes	and
T4624	21327-21330	NN	denotes	ALP
T4607	21331-21337	VBD	denotes	showed
T4625	21338-21344	JJ	denotes	little
T4626	21345-21355	NN	denotes	difference
T4627	21356-21361	IN	denotes	among
T4628	21362-21365	DT	denotes	the
T4629	21366-21372	NNS	denotes	groups
T4630	21372-21374	,	denotes	,
T4631	21374-21380	IN	denotes	except
T4632	21381-21384	IN	denotes	for
T4633	21385-21386	DT	denotes	a
T4635	21387-21392	JJ	denotes	small
T4634	21393-21401	NN	denotes	decrease
T4636	21402-21404	IN	denotes	in
T4637	21405-21407	CD	denotes	12
T4639	21407-21408	HYPH	denotes	-
T4638	21408-21413	NN	denotes	month
T4641	21413-21414	HYPH	denotes	-
T4640	21414-21417	JJ	denotes	old
T4643	21418-21423	NN	denotes	Sam68
T4644	21423-21424	SYM	denotes	−
T4645	21424-21425	HYPH	denotes	/
T4646	21425-21426	SYM	denotes	−
T4642	21427-21431	NNS	denotes	mice
T4647	21432-21435	CC	denotes	and
T4648	21436-21437	-LRB-	denotes	(
T4650	21437-21444	NN	denotes	Dataset
T4649	21445-21447	NN	denotes	S3
T4651	21447-21448	-RRB-	denotes	)
T4652	21448-21449	.	denotes	.
T4653	21449-21605	sentence	denotes	Serum estrogen was significantly decreased in all 12-month-old mice regardless of genotype with a reciprocal increase in interleukin-6 levels (Dataset S3).
T4654	21450-21455	NN	denotes	Serum
T4655	21456-21464	NN	denotes	estrogen
T4657	21465-21468	VBD	denotes	was
T4658	21469-21482	RB	denotes	significantly
T4656	21483-21492	VBN	denotes	decreased
T4659	21493-21495	IN	denotes	in
T4660	21496-21499	DT	denotes	all
T4662	21500-21502	CD	denotes	12
T4664	21502-21503	HYPH	denotes	-
T4663	21503-21508	NN	denotes	month
T4666	21508-21509	HYPH	denotes	-
T4665	21509-21512	JJ	denotes	old
T4661	21513-21517	NNS	denotes	mice
T4667	21518-21528	RB	denotes	regardless
T4668	21529-21531	IN	denotes	of
T4669	21532-21540	NN	denotes	genotype
T4670	21541-21545	IN	denotes	with
T4671	21546-21547	DT	denotes	a
T4673	21548-21558	JJ	denotes	reciprocal
T4672	21559-21567	NN	denotes	increase
T4674	21568-21570	IN	denotes	in
T4675	21571-21582	NN	denotes	interleukin
T4677	21582-21583	HYPH	denotes	-
T4678	21583-21584	CD	denotes	6
T4676	21585-21591	NNS	denotes	levels
T4679	21592-21593	-LRB-	denotes	(
T4681	21593-21600	NN	denotes	Dataset
T4680	21601-21603	NN	denotes	S3
T4682	21603-21604	-RRB-	denotes	)
T4683	21604-21605	.	denotes	.
T4684	21605-21728	sentence	denotes	Given the involvement of leptin in regulating body and bone mass, serum leptin was measured in Sam68+/+ and Sam68−/− mice.
T4685	21606-21611	VBN	denotes	Given
T4687	21612-21615	DT	denotes	the
T4688	21616-21627	NN	denotes	involvement
T4689	21628-21630	IN	denotes	of
T4690	21631-21637	NN	denotes	leptin
T4691	21638-21640	IN	denotes	in
T4692	21641-21651	VBG	denotes	regulating
T4693	21652-21656	NN	denotes	body
T4695	21657-21660	CC	denotes	and
T4696	21661-21665	NN	denotes	bone
T4694	21666-21670	NN	denotes	mass
T4697	21670-21672	,	denotes	,
T4698	21672-21677	NN	denotes	serum
T4699	21678-21684	NN	denotes	leptin
T4700	21685-21688	VBD	denotes	was
T4686	21689-21697	VBN	denotes	measured
T4701	21698-21700	IN	denotes	in
T4702	21701-21706	NN	denotes	Sam68
T4704	21706-21707	SYM	denotes	+
T4705	21707-21708	HYPH	denotes	/
T4706	21708-21709	SYM	denotes	+
T4707	21710-21713	CC	denotes	and
T4708	21714-21719	NN	denotes	Sam68
T4709	21719-21720	SYM	denotes	−
T4710	21720-21721	HYPH	denotes	/
T4711	21721-21722	SYM	denotes	−
T4703	21723-21727	NNS	denotes	mice
T4712	21727-21728	.	denotes	.
T4713	21728-21861	sentence	denotes	Twelve-month-old Sam68−/− mice had significantly lower levels than young and old Sam68+/+ mice and young Sam68−/− mice (Dataset S3).
T4714	21729-21735	CD	denotes	Twelve
T4716	21735-21736	HYPH	denotes	-
T4715	21736-21741	NN	denotes	month
T4718	21741-21742	HYPH	denotes	-
T4717	21742-21745	JJ	denotes	old
T4720	21746-21751	NN	denotes	Sam68
T4721	21751-21752	SYM	denotes	−
T4722	21752-21753	HYPH	denotes	/
T4723	21753-21754	SYM	denotes	−
T4719	21755-21759	NNS	denotes	mice
T4724	21760-21763	VBD	denotes	had
T4725	21764-21777	RB	denotes	significantly
T4726	21778-21783	JJR	denotes	lower
T4727	21784-21790	NNS	denotes	levels
T4728	21791-21795	IN	denotes	than
T4729	21796-21801	JJ	denotes	young
T4731	21802-21805	CC	denotes	and
T4732	21806-21809	JJ	denotes	old
T4733	21810-21815	NN	denotes	Sam68
T4734	21815-21816	SYM	denotes	+
T4735	21816-21817	HYPH	denotes	/
T4736	21817-21818	SYM	denotes	+
T4730	21819-21823	NNS	denotes	mice
T4737	21824-21827	CC	denotes	and
T4738	21828-21833	JJ	denotes	young
T4740	21834-21839	NN	denotes	Sam68
T4741	21839-21840	SYM	denotes	−
T4742	21840-21841	HYPH	denotes	/
T4743	21841-21842	SYM	denotes	−
T4739	21843-21847	NNS	denotes	mice
T4744	21848-21849	-LRB-	denotes	(
T4746	21849-21856	NN	denotes	Dataset
T4745	21857-21859	NN	denotes	S3
T4747	21859-21860	-RRB-	denotes	)
T4748	21860-21861	.	denotes	.
T4749	21861-22083	sentence	denotes	Taken together, these observations suggested that preservation of bone mass in the Sam68−/− mice was not due primarily to altered estrogen status but could have been influenced by differences in circulating leptin levels.
T4750	21862-21867	VBN	denotes	Taken
T4752	21868-21876	RB	denotes	together
T4753	21876-21878	,	denotes	,
T4754	21878-21883	DT	denotes	these
T4755	21884-21896	NNS	denotes	observations
T4751	21897-21906	VBD	denotes	suggested
T4756	21907-21911	IN	denotes	that
T4758	21912-21924	NN	denotes	preservation
T4759	21925-21927	IN	denotes	of
T4760	21928-21932	NN	denotes	bone
T4761	21933-21937	NN	denotes	mass
T4762	21938-21940	IN	denotes	in
T4763	21941-21944	DT	denotes	the
T4765	21945-21950	NN	denotes	Sam68
T4766	21950-21951	SYM	denotes	−
T4767	21951-21952	HYPH	denotes	/
T4768	21952-21953	SYM	denotes	−
T4764	21954-21958	NNS	denotes	mice
T4757	21959-21962	VBD	denotes	was
T4769	21963-21966	RB	denotes	not
T4770	21967-21970	IN	denotes	due
T4771	21971-21980	RB	denotes	primarily
T4772	21981-21983	IN	denotes	to
T4773	21984-21991	VBN	denotes	altered
T4775	21992-22000	NN	denotes	estrogen
T4774	22001-22007	NN	denotes	status
T4776	22008-22011	CC	denotes	but
T4777	22012-22017	MD	denotes	could
T4779	22018-22022	VB	denotes	have
T4780	22023-22027	VBN	denotes	been
T4778	22028-22038	VBN	denotes	influenced
T4781	22039-22041	IN	denotes	by
T4782	22042-22053	NNS	denotes	differences
T4783	22054-22056	IN	denotes	in
T4784	22057-22068	VBG	denotes	circulating
T4786	22069-22075	NN	denotes	leptin
T4785	22076-22082	NNS	denotes	levels
T4787	22082-22083	.	denotes	.
T4956	22085-22095	NN	denotes	Osteoblast
T4958	22095-22097	,	denotes	,
T4959	22097-22100	CC	denotes	but
T4960	22101-22104	RB	denotes	Not
T4961	22105-22115	NN	denotes	Osteoclast
T4962	22115-22117	,	denotes	,
T4957	22117-22125	NN	denotes	Activity
T4964	22126-22128	VBZ	denotes	Is
T4963	22129-22136	VBN	denotes	Altered
T4965	22137-22139	IN	denotes	in
T4966	22140-22145	NN	denotes	Sam68
T4968	22145-22146	SYM	denotes	−
T4969	22146-22147	HYPH	denotes	/
T4970	22147-22148	SYM	denotes	−
T4967	22149-22153	NNS	denotes	Mice
T4971	22154-22156	FW	denotes	Ex
T4972	22157-22161	FW	denotes	Vivo
T4973	22161-22340	sentence	denotes	Maintenance of bone mass during the adult remodeling cycle is dependent on the coupling of osteoblast to osteoclast activity, such that there is no net gain or loss of bone [49].
T4974	22162-22173	NN	denotes	Maintenance
T4976	22174-22176	IN	denotes	of
T4977	22177-22181	NN	denotes	bone
T4978	22182-22186	NN	denotes	mass
T4979	22187-22193	IN	denotes	during
T4980	22194-22197	DT	denotes	the
T4982	22198-22203	JJ	denotes	adult
T4983	22204-22214	NN	denotes	remodeling
T4981	22215-22220	NN	denotes	cycle
T4975	22221-22223	VBZ	denotes	is
T4984	22224-22233	JJ	denotes	dependent
T4985	22234-22236	IN	denotes	on
T4986	22237-22240	DT	denotes	the
T4987	22241-22249	NN	denotes	coupling
T4988	22250-22252	IN	denotes	of
T4989	22253-22263	NN	denotes	osteoblast
T4990	22264-22266	IN	denotes	to
T4991	22267-22277	NN	denotes	osteoclast
T4992	22278-22286	NN	denotes	activity
T4993	22286-22288	,	denotes	,
T4994	22288-22292	JJ	denotes	such
T4996	22293-22297	IN	denotes	that
T4997	22298-22303	EX	denotes	there
T4995	22304-22306	VBZ	denotes	is
T4998	22307-22309	DT	denotes	no
T5000	22310-22313	JJ	denotes	net
T4999	22314-22318	NN	denotes	gain
T5001	22319-22321	CC	denotes	or
T5002	22322-22326	NN	denotes	loss
T5003	22327-22329	IN	denotes	of
T5004	22330-22334	NN	denotes	bone
T5005	22335-22336	-LRB-	denotes	[
T5006	22336-22338	CD	denotes	49
T5007	22338-22339	-RRB-	denotes	]
T5008	22339-22340	.	denotes	.
T5009	22340-22541	sentence	denotes	To further explore the age-related advantage of Sam68−/− mice with respect to bone preservation, we examined the functional activity of Sam68−/− osteoblasts and osteoclasts ex vivo (Figure 6A and 6B).
T5010	22341-22343	TO	denotes	To
T5012	22344-22351	RB	denotes	further
T5011	22352-22359	VB	denotes	explore
T5014	22360-22363	DT	denotes	the
T5016	22364-22367	NN	denotes	age
T5018	22367-22368	HYPH	denotes	-
T5017	22368-22375	VBN	denotes	related
T5015	22376-22385	NN	denotes	advantage
T5019	22386-22388	IN	denotes	of
T5020	22389-22394	NN	denotes	Sam68
T5022	22394-22395	SYM	denotes	−
T5023	22395-22396	HYPH	denotes	/
T5024	22396-22397	SYM	denotes	−
T5021	22398-22402	NNS	denotes	mice
T5025	22403-22407	IN	denotes	with
T5026	22408-22415	NN	denotes	respect
T5027	22416-22418	IN	denotes	to
T5028	22419-22423	NN	denotes	bone
T5029	22424-22436	NN	denotes	preservation
T5030	22436-22438	,	denotes	,
T5031	22438-22440	PRP	denotes	we
T5013	22441-22449	VBD	denotes	examined
T5032	22450-22453	DT	denotes	the
T5034	22454-22464	JJ	denotes	functional
T5033	22465-22473	NN	denotes	activity
T5035	22474-22476	IN	denotes	of
T5036	22477-22482	NN	denotes	Sam68
T5037	22482-22483	SYM	denotes	−
T5038	22483-22484	HYPH	denotes	/
T5039	22484-22485	SYM	denotes	−
T5040	22486-22497	NNS	denotes	osteoblasts
T5041	22498-22501	CC	denotes	and
T5042	22502-22513	NNS	denotes	osteoclasts
T5043	22514-22516	FW	denotes	ex
T5044	22517-22521	FW	denotes	vivo
T5045	22522-22523	-LRB-	denotes	(
T5047	22523-22529	NN	denotes	Figure
T5046	22530-22532	NN	denotes	6A
T5048	22533-22536	CC	denotes	and
T5049	22537-22539	NN	denotes	6B
T5050	22539-22540	-RRB-	denotes	)
T5051	22540-22541	.	denotes	.
T5052	22541-22964	sentence	denotes	Cultures of bone marrow stromal cells harvested from 4-week-old Sam68−/− mice and maintained for 18 days in osteoblast differentiation medium demonstrated more intense staining for ALP at 6 days (unpublished data) and 18 days (Figure 6A), and more mineralized nodules at 18 days, than the Sam68+/+ mice, even though similar amounts of fibroblast colony-forming units (CFU-F) were observed (Figure 6A and unpublished data).
T5053	22542-22550	NNS	denotes	Cultures
T5055	22551-22553	IN	denotes	of
T5056	22554-22558	NN	denotes	bone
T5057	22559-22565	NN	denotes	marrow
T5059	22566-22573	JJ	denotes	stromal
T5058	22574-22579	NNS	denotes	cells
T5060	22580-22589	VBN	denotes	harvested
T5061	22590-22594	IN	denotes	from
T5062	22595-22596	CD	denotes	4
T5064	22596-22597	HYPH	denotes	-
T5063	22597-22601	NN	denotes	week
T5066	22601-22602	HYPH	denotes	-
T5065	22602-22605	JJ	denotes	old
T5068	22606-22611	NN	denotes	Sam68
T5069	22611-22612	SYM	denotes	−
T5070	22612-22613	HYPH	denotes	/
T5071	22613-22614	SYM	denotes	−
T5067	22615-22619	NNS	denotes	mice
T5072	22620-22623	CC	denotes	and
T5073	22624-22634	VBN	denotes	maintained
T5074	22635-22638	IN	denotes	for
T5075	22639-22641	CD	denotes	18
T5076	22642-22646	NNS	denotes	days
T5077	22647-22649	IN	denotes	in
T5078	22650-22660	NN	denotes	osteoblast
T5079	22661-22676	NN	denotes	differentiation
T5080	22677-22683	NN	denotes	medium
T5054	22684-22696	VBD	denotes	demonstrated
T5081	22697-22701	RBR	denotes	more
T5082	22702-22709	JJ	denotes	intense
T5083	22710-22718	NN	denotes	staining
T5084	22719-22722	IN	denotes	for
T5085	22723-22726	NN	denotes	ALP
T5086	22727-22729	IN	denotes	at
T5087	22730-22731	CD	denotes	6
T5088	22732-22736	NNS	denotes	days
T5089	22737-22738	-LRB-	denotes	(
T5091	22738-22749	JJ	denotes	unpublished
T5090	22750-22754	NNS	denotes	data
T5092	22754-22755	-RRB-	denotes	)
T5093	22756-22759	CC	denotes	and
T5094	22760-22762	CD	denotes	18
T5095	22763-22767	NNS	denotes	days
T5096	22768-22769	-LRB-	denotes	(
T5098	22769-22775	NN	denotes	Figure
T5097	22776-22778	NN	denotes	6A
T5099	22778-22779	NN	denotes	)
T5100	22779-22781	NN	denotes	,
T5101	22781-22784	CC	denotes	and
T5102	22785-22789	JJR	denotes	more
T5104	22790-22801	VBN	denotes	mineralized
T5103	22802-22809	NNS	denotes	nodules
T5105	22810-22812	IN	denotes	at
T5106	22813-22815	CD	denotes	18
T5107	22816-22820	NNS	denotes	days
T5108	22820-22822	NN	denotes	,
T5109	22822-22826	IN	denotes	than
T5110	22827-22830	DT	denotes	the
T5112	22831-22836	NN	denotes	Sam68
T5113	22836-22837	SYM	denotes	+
T5114	22837-22838	HYPH	denotes	/
T5115	22838-22839	SYM	denotes	+
T5111	22840-22844	NNS	denotes	mice
T5116	22844-22846	NN	denotes	,
T5117	22846-22850	RB	denotes	even
T5119	22851-22857	IN	denotes	though
T5120	22858-22865	JJ	denotes	similar
T5121	22866-22873	NNS	denotes	amounts
T5122	22874-22876	IN	denotes	of
T5123	22877-22887	NN	denotes	fibroblast
T5125	22888-22894	NN	denotes	colony
T5127	22894-22895	HYPH	denotes	-
T5126	22895-22902	VBG	denotes	forming
T5124	22903-22908	NNS	denotes	units
T5128	22909-22910	-LRB-	denotes	(
T5129	22910-22913	NN	denotes	CFU
T5131	22913-22914	HYPH	denotes	-
T5130	22914-22915	NN	denotes	F
T5132	22915-22916	-RRB-	denotes	)
T5133	22917-22921	VBD	denotes	were
T5118	22922-22930	VBN	denotes	observed
T5134	22931-22932	-LRB-	denotes	(
T5136	22932-22938	NN	denotes	Figure
T5135	22939-22941	NN	denotes	6A
T5137	22942-22945	CC	denotes	and
T5138	22946-22957	JJ	denotes	unpublished
T5139	22958-22962	NNS	denotes	data
T5140	22962-22963	-RRB-	denotes	)
T5141	22963-22964	.	denotes	.
T5142	22964-23215	sentence	denotes	RT-PCR analysis of molecular markers of osteoblast differentiation (Dataset S2) revealed similar increases over time in RNA from Sam68+/+ and Sam68−/− mice, although type I collagen appeared to be up-regulated at both timepoints in the Sam68−/− mice.
T5143	22965-22967	NN	denotes	RT
T5145	22967-22968	HYPH	denotes	-
T5144	22968-22971	NN	denotes	PCR
T5146	22972-22980	NN	denotes	analysis
T5148	22981-22983	IN	denotes	of
T5149	22984-22993	JJ	denotes	molecular
T5150	22994-23001	NNS	denotes	markers
T5151	23002-23004	IN	denotes	of
T5152	23005-23015	NN	denotes	osteoblast
T5153	23016-23031	NN	denotes	differentiation
T5154	23032-23033	-LRB-	denotes	(
T5156	23033-23040	NN	denotes	Dataset
T5155	23041-23043	NN	denotes	S2
T5157	23043-23044	-RRB-	denotes	)
T5147	23045-23053	VBD	denotes	revealed
T5158	23054-23061	JJ	denotes	similar
T5159	23062-23071	NNS	denotes	increases
T5160	23072-23076	IN	denotes	over
T5161	23077-23081	NN	denotes	time
T5162	23082-23084	IN	denotes	in
T5163	23085-23088	NN	denotes	RNA
T5164	23089-23093	IN	denotes	from
T5165	23094-23099	NN	denotes	Sam68
T5167	23099-23100	SYM	denotes	+
T5168	23100-23101	HYPH	denotes	/
T5169	23101-23102	SYM	denotes	+
T5170	23103-23106	CC	denotes	and
T5171	23107-23112	NN	denotes	Sam68
T5172	23112-23113	SYM	denotes	−
T5173	23113-23114	HYPH	denotes	/
T5174	23114-23115	SYM	denotes	−
T5166	23116-23120	NNS	denotes	mice
T5175	23120-23122	,	denotes	,
T5176	23122-23130	IN	denotes	although
T5178	23131-23135	NN	denotes	type
T5180	23136-23137	CD	denotes	I
T5179	23138-23146	NN	denotes	collagen
T5177	23147-23155	VBD	denotes	appeared
T5181	23156-23158	TO	denotes	to
T5183	23159-23161	VB	denotes	be
T5184	23162-23164	RB	denotes	up
T5185	23164-23165	HYPH	denotes	-
T5182	23165-23174	VBN	denotes	regulated
T5186	23175-23177	IN	denotes	at
T5187	23178-23182	DT	denotes	both
T5188	23183-23193	NNS	denotes	timepoints
T5189	23194-23196	IN	denotes	in
T5190	23197-23200	DT	denotes	the
T5192	23201-23206	NN	denotes	Sam68
T5193	23206-23207	SYM	denotes	−
T5194	23207-23208	HYPH	denotes	/
T5195	23208-23209	SYM	denotes	−
T5191	23210-23214	NNS	denotes	mice
T5196	23214-23215	.	denotes	.
T5197	23215-23495	sentence	denotes	Short-term cultures of mature osteoclasts released from the crushed long bones of Sam68+/+ and Sam68−/− mice stained equally well for TRAP and excavated approximately equal numbers of pits of equal size in dentin slices, as visualized by scanning electron microscopy (Figure 6B).
T5198	23216-23221	JJ	denotes	Short
T5200	23221-23222	HYPH	denotes	-
T5199	23222-23226	NN	denotes	term
T5201	23227-23235	NNS	denotes	cultures
T5203	23236-23238	IN	denotes	of
T5204	23239-23245	JJ	denotes	mature
T5205	23246-23257	NNS	denotes	osteoclasts
T5206	23258-23266	VBN	denotes	released
T5207	23267-23271	IN	denotes	from
T5208	23272-23275	DT	denotes	the
T5210	23276-23283	JJ	denotes	crushed
T5211	23284-23288	JJ	denotes	long
T5209	23289-23294	NNS	denotes	bones
T5212	23295-23297	IN	denotes	of
T5213	23298-23303	NN	denotes	Sam68
T5215	23303-23304	SYM	denotes	+
T5216	23304-23305	HYPH	denotes	/
T5217	23305-23306	SYM	denotes	+
T5218	23307-23310	CC	denotes	and
T5219	23311-23316	NN	denotes	Sam68
T5220	23316-23317	SYM	denotes	−
T5221	23317-23318	HYPH	denotes	/
T5222	23318-23319	SYM	denotes	−
T5214	23320-23324	NNS	denotes	mice
T5202	23325-23332	VBD	denotes	stained
T5223	23333-23340	RB	denotes	equally
T5224	23341-23345	RB	denotes	well
T5225	23346-23349	IN	denotes	for
T5226	23350-23354	NN	denotes	TRAP
T5227	23355-23358	CC	denotes	and
T5228	23359-23368	VBD	denotes	excavated
T5229	23369-23382	RB	denotes	approximately
T5230	23383-23388	JJ	denotes	equal
T5231	23389-23396	NNS	denotes	numbers
T5232	23397-23399	IN	denotes	of
T5233	23400-23404	NNS	denotes	pits
T5234	23405-23407	IN	denotes	of
T5235	23408-23413	JJ	denotes	equal
T5236	23414-23418	NN	denotes	size
T5237	23419-23421	IN	denotes	in
T5238	23422-23428	NN	denotes	dentin
T5239	23429-23435	NNS	denotes	slices
T5240	23435-23437	,	denotes	,
T5241	23437-23439	IN	denotes	as
T5242	23440-23450	VBN	denotes	visualized
T5243	23451-23453	IN	denotes	by
T5244	23454-23462	VBG	denotes	scanning
T5245	23463-23471	NN	denotes	electron
T5246	23472-23482	NN	denotes	microscopy
T5247	23483-23484	-LRB-	denotes	(
T5249	23484-23490	NN	denotes	Figure
T5248	23491-23493	NN	denotes	6B
T5250	23493-23494	-RRB-	denotes	)
T5251	23494-23495	.	denotes	.
T5252	23495-23622	sentence	denotes	These findings suggest that the osteoclasts may not be the primary defect in Sam68−/− mice, as they are in Src null mice [48].
T5253	23496-23501	DT	denotes	These
T5254	23502-23510	NNS	denotes	findings
T5255	23511-23518	VBP	denotes	suggest
T5256	23519-23523	IN	denotes	that
T5258	23524-23527	DT	denotes	the
T5259	23528-23539	NNS	denotes	osteoclasts
T5260	23540-23543	MD	denotes	may
T5261	23544-23547	RB	denotes	not
T5257	23548-23550	VB	denotes	be
T5262	23551-23554	DT	denotes	the
T5264	23555-23562	JJ	denotes	primary
T5263	23563-23569	NN	denotes	defect
T5265	23570-23572	IN	denotes	in
T5266	23573-23578	NN	denotes	Sam68
T5268	23578-23579	SYM	denotes	−
T5269	23579-23580	HYPH	denotes	/
T5270	23580-23581	SYM	denotes	−
T5267	23582-23586	NNS	denotes	mice
T5271	23586-23588	,	denotes	,
T5272	23588-23590	IN	denotes	as
T5274	23591-23595	PRP	denotes	they
T5273	23596-23599	VBP	denotes	are
T5275	23600-23602	IN	denotes	in
T5276	23603-23606	NN	denotes	Src
T5278	23607-23611	JJ	denotes	null
T5277	23612-23616	NNS	denotes	mice
T5279	23617-23618	-LRB-	denotes	[
T5280	23618-23620	CD	denotes	48
T5281	23620-23621	-RRB-	denotes	]
T5282	23621-23622	.	denotes	.
T5689	24688-24691	DT	denotes	The
T5690	24692-24699	NN	denotes	Absence
T5692	24700-24702	IN	denotes	of
T5693	24703-24708	NN	denotes	Sam68
T5691	24709-24717	VBZ	denotes	Prevents
T5694	24718-24727	NN	denotes	Adipocyte
T5695	24728-24743	NN	denotes	Differentiation
T5696	24744-24747	CC	denotes	and
T5697	24748-24756	VBZ	denotes	Promotes
T5698	24757-24767	NN	denotes	Osteoblast
T5699	24768-24783	NN	denotes	Differentiation
T5700	24783-24994	sentence	denotes	It is well recognized that bone marrow stromal cells give rise to both osteoblasts and adipocytes and that age-related bone loss is accompanied by an increase in differentiation down the adipocyte lineage [22].
T5701	24784-24786	PRP	denotes	It
T5703	24787-24789	VBZ	denotes	is
T5704	24790-24794	RB	denotes	well
T5702	24795-24805	VBN	denotes	recognized
T5705	24806-24810	IN	denotes	that
T5707	24811-24815	NN	denotes	bone
T5708	24816-24822	NN	denotes	marrow
T5710	24823-24830	JJ	denotes	stromal
T5709	24831-24836	NNS	denotes	cells
T5706	24837-24841	VBP	denotes	give
T5711	24842-24846	NN	denotes	rise
T5712	24847-24849	IN	denotes	to
T5713	24850-24854	DT	denotes	both
T5714	24855-24866	NNS	denotes	osteoblasts
T5715	24867-24870	CC	denotes	and
T5716	24871-24881	NNS	denotes	adipocytes
T5717	24882-24885	CC	denotes	and
T5718	24886-24890	IN	denotes	that
T5720	24891-24894	NN	denotes	age
T5722	24894-24895	HYPH	denotes	-
T5721	24895-24902	VBN	denotes	related
T5724	24903-24907	NN	denotes	bone
T5723	24908-24912	NN	denotes	loss
T5725	24913-24915	VBZ	denotes	is
T5719	24916-24927	VBN	denotes	accompanied
T5726	24928-24930	IN	denotes	by
T5727	24931-24933	DT	denotes	an
T5728	24934-24942	NN	denotes	increase
T5729	24943-24945	IN	denotes	in
T5730	24946-24961	NN	denotes	differentiation
T5731	24962-24966	IN	denotes	down
T5732	24967-24970	DT	denotes	the
T5734	24971-24980	NN	denotes	adipocyte
T5733	24981-24988	NN	denotes	lineage
T5735	24989-24990	-LRB-	denotes	[
T5736	24990-24992	CD	denotes	22
T5737	24992-24993	-RRB-	denotes	]
T5738	24993-24994	.	denotes	.
T5739	24994-25138	sentence	denotes	Therefore, the loss of Sam68 could influence the bone marrow stromal cells to differentiate along the osteogenic versus the adipogenic pathway.
T5740	24995-25004	RB	denotes	Therefore
T5742	25004-25006	,	denotes	,
T5743	25006-25009	DT	denotes	the
T5744	25010-25014	NN	denotes	loss
T5745	25015-25017	IN	denotes	of
T5746	25018-25023	NN	denotes	Sam68
T5747	25024-25029	MD	denotes	could
T5741	25030-25039	VB	denotes	influence
T5748	25040-25043	DT	denotes	the
T5750	25044-25048	NN	denotes	bone
T5751	25049-25055	NN	denotes	marrow
T5752	25056-25063	JJ	denotes	stromal
T5749	25064-25069	NNS	denotes	cells
T5753	25070-25072	TO	denotes	to
T5754	25073-25086	VB	denotes	differentiate
T5755	25087-25092	IN	denotes	along
T5756	25093-25096	DT	denotes	the
T5757	25097-25107	JJ	denotes	osteogenic
T5758	25108-25114	CC	denotes	versus
T5759	25115-25118	DT	denotes	the
T5760	25119-25129	JJ	denotes	adipogenic
T5761	25130-25137	NN	denotes	pathway
T5762	25137-25138	.	denotes	.
T5763	25138-25372	sentence	denotes	Alternatively, the loss of Sam68 could indirectly regulate bone mass as a general disturbance of neuroendocrine control as was shown when leptin and the sympathetic nervous system axis were shown to negatively regulate bone mass [8].
T5764	25139-25152	RB	denotes	Alternatively
T5766	25152-25154	,	denotes	,
T5767	25154-25157	DT	denotes	the
T5768	25158-25162	NN	denotes	loss
T5769	25163-25165	IN	denotes	of
T5770	25166-25171	NN	denotes	Sam68
T5771	25172-25177	MD	denotes	could
T5772	25178-25188	RB	denotes	indirectly
T5765	25189-25197	VB	denotes	regulate
T5773	25198-25202	NN	denotes	bone
T5774	25203-25207	NN	denotes	mass
T5775	25208-25210	IN	denotes	as
T5776	25211-25212	DT	denotes	a
T5778	25213-25220	JJ	denotes	general
T5777	25221-25232	NN	denotes	disturbance
T5779	25233-25235	IN	denotes	of
T5780	25236-25250	JJ	denotes	neuroendocrine
T5781	25251-25258	NN	denotes	control
T5782	25259-25261	IN	denotes	as
T5784	25262-25265	VBD	denotes	was
T5783	25266-25271	VBN	denotes	shown
T5785	25272-25276	WRB	denotes	when
T5787	25277-25283	NN	denotes	leptin
T5788	25284-25287	CC	denotes	and
T5789	25288-25291	DT	denotes	the
T5791	25292-25303	JJ	denotes	sympathetic
T5792	25304-25311	JJ	denotes	nervous
T5793	25312-25318	NN	denotes	system
T5790	25319-25323	NN	denotes	axis
T5794	25324-25328	VBD	denotes	were
T5786	25329-25334	VBN	denotes	shown
T5795	25335-25337	TO	denotes	to
T5797	25338-25348	RB	denotes	negatively
T5796	25349-25357	VB	denotes	regulate
T5798	25358-25362	NN	denotes	bone
T5799	25363-25367	NN	denotes	mass
T5800	25368-25369	-LRB-	denotes	[
T5801	25369-25370	CD	denotes	8
T5802	25370-25371	-RRB-	denotes	]
T5803	25371-25372	.	denotes	.
T5804	25372-25522	sentence	denotes	To confirm a role for Sam68 in the regulation of adipocyte differentiation, we isolated primary MEFs from 14.5-day-old Sam68+/+ and Sam68−/− embryos.
T5805	25373-25375	TO	denotes	To
T5806	25376-25383	VB	denotes	confirm
T5808	25384-25385	DT	denotes	a
T5809	25386-25390	NN	denotes	role
T5810	25391-25394	IN	denotes	for
T5811	25395-25400	NN	denotes	Sam68
T5812	25401-25403	IN	denotes	in
T5813	25404-25407	DT	denotes	the
T5814	25408-25418	NN	denotes	regulation
T5815	25419-25421	IN	denotes	of
T5816	25422-25431	NN	denotes	adipocyte
T5817	25432-25447	NN	denotes	differentiation
T5818	25447-25449	,	denotes	,
T5819	25449-25451	PRP	denotes	we
T5807	25452-25460	VBD	denotes	isolated
T5820	25461-25468	JJ	denotes	primary
T5821	25469-25473	NNS	denotes	MEFs
T5822	25474-25478	IN	denotes	from
T5823	25479-25483	CD	denotes	14.5
T5825	25483-25484	HYPH	denotes	-
T5824	25484-25487	NN	denotes	day
T5827	25487-25488	HYPH	denotes	-
T5826	25488-25491	JJ	denotes	old
T5829	25492-25497	NN	denotes	Sam68
T5830	25497-25498	SYM	denotes	+
T5831	25498-25499	HYPH	denotes	/
T5832	25499-25500	SYM	denotes	+
T5833	25501-25504	CC	denotes	and
T5834	25505-25510	NN	denotes	Sam68
T5835	25510-25511	SYM	denotes	−
T5836	25511-25512	HYPH	denotes	/
T5837	25512-25513	SYM	denotes	−
T5828	25514-25521	NNS	denotes	embryos
T5838	25521-25522	.	denotes	.
T5839	25522-25664	sentence	denotes	The primary Sam68−/− MEFs were differentiated into adipocytes in vitro in culture medium containing 5 μM pioglitazone to induce adipogenesis.
T5840	25523-25526	DT	denotes	The
T5842	25527-25534	JJ	denotes	primary
T5843	25535-25540	NN	denotes	Sam68
T5844	25540-25541	SYM	denotes	−
T5845	25541-25542	HYPH	denotes	/
T5846	25542-25543	SYM	denotes	−
T5841	25544-25548	NNS	denotes	MEFs
T5848	25549-25553	VBD	denotes	were
T5847	25554-25568	VBN	denotes	differentiated
T5849	25569-25573	IN	denotes	into
T5850	25574-25584	NNS	denotes	adipocytes
T5851	25585-25587	FW	denotes	in
T5852	25588-25593	FW	denotes	vitro
T5853	25594-25596	IN	denotes	in
T5854	25597-25604	NN	denotes	culture
T5855	25605-25611	NN	denotes	medium
T5856	25612-25622	VBG	denotes	containing
T5857	25623-25624	CD	denotes	5
T5858	25625-25627	NN	denotes	μM
T5859	25628-25640	NN	denotes	pioglitazone
T5860	25641-25643	TO	denotes	to
T5861	25644-25650	VB	denotes	induce
T5862	25651-25663	NN	denotes	adipogenesis
T5863	25663-25664	.	denotes	.
T5864	25664-25747	sentence	denotes	Cells at days 0, 4, 6, and 12 were stained with Oil red O to monitor adipogenesis.
T5865	25665-25670	NNS	denotes	Cells
T5867	25671-25673	IN	denotes	at
T5868	25674-25678	NNS	denotes	days
T5869	25679-25680	CD	denotes	0
T5870	25680-25682	,	denotes	,
T5871	25682-25683	CD	denotes	4
T5872	25683-25685	,	denotes	,
T5873	25685-25686	CD	denotes	6
T5874	25686-25688	,	denotes	,
T5875	25688-25691	CC	denotes	and
T5876	25692-25694	CD	denotes	12
T5877	25695-25699	VBD	denotes	were
T5866	25700-25707	VBN	denotes	stained
T5878	25708-25712	IN	denotes	with
T5879	25713-25716	NN	denotes	Oil
T5881	25717-25720	NN	denotes	red
T5880	25721-25722	NN	denotes	O
T5882	25723-25725	TO	denotes	to
T5883	25726-25733	VB	denotes	monitor
T5884	25734-25746	NN	denotes	adipogenesis
T5885	25746-25747	.	denotes	.
T5886	25747-25933	sentence	denotes	Adipogenesis was more pronounced in the wild-type MEFs cultures than in the Sam68−/− MEFs, consistent with the positive role of endogenous Sam68 in adipocyte differentiation (Figure 7).
T5887	25748-25760	NN	denotes	Adipogenesis
T5888	25761-25764	VBD	denotes	was
T5889	25765-25769	RBR	denotes	more
T5890	25770-25780	JJ	denotes	pronounced
T5891	25781-25783	IN	denotes	in
T5892	25784-25787	DT	denotes	the
T5894	25788-25792	JJ	denotes	wild
T5896	25792-25793	HYPH	denotes	-
T5895	25793-25797	NN	denotes	type
T5897	25798-25802	NNS	denotes	MEFs
T5893	25803-25811	NNS	denotes	cultures
T5898	25812-25816	IN	denotes	than
T5899	25817-25819	IN	denotes	in
T5900	25820-25823	DT	denotes	the
T5902	25824-25829	NN	denotes	Sam68
T5903	25829-25830	SYM	denotes	−
T5904	25830-25831	HYPH	denotes	/
T5905	25831-25832	SYM	denotes	−
T5901	25833-25837	NNS	denotes	MEFs
T5906	25837-25839	,	denotes	,
T5907	25839-25849	JJ	denotes	consistent
T5908	25850-25854	IN	denotes	with
T5909	25855-25858	DT	denotes	the
T5911	25859-25867	JJ	denotes	positive
T5910	25868-25872	NN	denotes	role
T5912	25873-25875	IN	denotes	of
T5913	25876-25886	JJ	denotes	endogenous
T5914	25887-25892	NN	denotes	Sam68
T5915	25893-25895	IN	denotes	in
T5916	25896-25905	NN	denotes	adipocyte
T5917	25906-25921	NN	denotes	differentiation
T5918	25922-25923	-LRB-	denotes	(
T5919	25923-25929	NN	denotes	Figure
T5920	25930-25931	CD	denotes	7
T5921	25931-25932	-RRB-	denotes	)
T5922	25932-25933	.	denotes	.
T5923	25933-26154	sentence	denotes	The expression of key transcription factors including the PPARγ and KLF5 was impaired in Sam68−/− differentiated MEFs compared with Sam68+/+ MEFs, consistent with impaired adipogenesis in the absence of Sam68 (Figure 7).
T5924	25934-25937	DT	denotes	The
T5925	25938-25948	NN	denotes	expression
T5927	25949-25951	IN	denotes	of
T5928	25952-25955	JJ	denotes	key
T5930	25956-25969	NN	denotes	transcription
T5929	25970-25977	NNS	denotes	factors
T5931	25978-25987	VBG	denotes	including
T5932	25988-25991	DT	denotes	the
T5933	25992-25997	NN	denotes	PPARγ
T5934	25998-26001	CC	denotes	and
T5935	26002-26006	NN	denotes	KLF5
T5936	26007-26010	VBD	denotes	was
T5926	26011-26019	VBN	denotes	impaired
T5937	26020-26022	IN	denotes	in
T5938	26023-26028	NN	denotes	Sam68
T5940	26028-26029	SYM	denotes	−
T5941	26029-26030	HYPH	denotes	/
T5942	26030-26031	SYM	denotes	−
T5943	26032-26046	VBN	denotes	differentiated
T5939	26047-26051	NNS	denotes	MEFs
T5944	26052-26060	VBN	denotes	compared
T5945	26061-26065	IN	denotes	with
T5946	26066-26071	NN	denotes	Sam68
T5948	26071-26072	SYM	denotes	+
T5949	26072-26073	HYPH	denotes	/
T5950	26073-26074	SYM	denotes	+
T5947	26075-26079	NNS	denotes	MEFs
T5951	26079-26081	,	denotes	,
T5952	26081-26091	JJ	denotes	consistent
T5953	26092-26096	IN	denotes	with
T5954	26097-26105	VBN	denotes	impaired
T5955	26106-26118	NN	denotes	adipogenesis
T5956	26119-26121	IN	denotes	in
T5957	26122-26125	DT	denotes	the
T5958	26126-26133	NN	denotes	absence
T5959	26134-26136	IN	denotes	of
T5960	26137-26142	NN	denotes	Sam68
T5961	26143-26144	-LRB-	denotes	(
T5962	26144-26150	NN	denotes	Figure
T5963	26151-26152	CD	denotes	7
T5964	26152-26153	-RRB-	denotes	)
T5965	26153-26154	.	denotes	.
T5966	26154-26362	sentence	denotes	These data, together with data confirming a lean phenotype in Sam68−/− mice (N. Torabi and S. Richard, unpublished data), support the hypothesis that Sam68 modulates the differentiation of mesenchymal cells.
T5967	26155-26160	DT	denotes	These
T5968	26161-26165	NNS	denotes	data
T5970	26165-26167	,	denotes	,
T5971	26167-26175	RB	denotes	together
T5972	26176-26180	IN	denotes	with
T5973	26181-26185	NNS	denotes	data
T5974	26186-26196	VBG	denotes	confirming
T5975	26197-26198	DT	denotes	a
T5977	26199-26203	JJ	denotes	lean
T5976	26204-26213	NN	denotes	phenotype
T5978	26214-26216	IN	denotes	in
T5979	26217-26222	NN	denotes	Sam68
T5981	26222-26223	SYM	denotes	−
T5982	26223-26224	HYPH	denotes	/
T5983	26224-26225	SYM	denotes	−
T5980	26226-26230	NNS	denotes	mice
T5984	26231-26232	-LRB-	denotes	(
T5985	26232-26234	NNP	denotes	N.
T5986	26235-26241	NNP	denotes	Torabi
T5987	26242-26245	CC	denotes	and
T5988	26246-26248	NNP	denotes	S.
T5989	26249-26256	NNP	denotes	Richard
T5990	26256-26258	,	denotes	,
T5991	26258-26269	JJ	denotes	unpublished
T5992	26270-26274	NNS	denotes	data
T5993	26274-26275	-RRB-	denotes	)
T5994	26275-26277	,	denotes	,
T5969	26277-26284	VBP	denotes	support
T5995	26285-26288	DT	denotes	the
T5996	26289-26299	NN	denotes	hypothesis
T5997	26300-26304	IN	denotes	that
T5999	26305-26310	NN	denotes	Sam68
T5998	26311-26320	VBZ	denotes	modulates
T6000	26321-26324	DT	denotes	the
T6001	26325-26340	NN	denotes	differentiation
T6002	26341-26343	IN	denotes	of
T6003	26344-26355	JJ	denotes	mesenchymal
T6004	26356-26361	NNS	denotes	cells
T6005	26361-26362	.	denotes	.
T6006	26362-27428	sentence	denotes	Figure 7 Ex Vivo Adipogenesis Analysis of Sam68−/− Mouse Embryonic Fibroblasts MEFs were isolated from mouse embryos at embryonic day 14.5. Equal number of MEFs from Sam68+/+ and Sam68−/− was plated on glass cover slips in 24 well-plates. Adipocyte differentiation was carried out at indicated times by the addition of complete media containing the pioglitazone. (A) Cultures were fixed in 4% paraformaldehyde and stained with Oil Red O to detect the fat droplets stored in adipocytes and photographed (top). The cell images were magnified ×10 and ×20 as indicated. (B) RT-PCR was carried out on total cellular RNA isolated after differentiation of the MEFs for day 0, 2, 4, 6, and 12. The DNA fragments were visualized on agarose gels stained with ethidium bromide. The expression of adipogenic markers C/EBPβ, C/EBPδ, PPARα, and KLF5 was examined as well as the expression of controls including Sam68, β-actin, and GAPDH. To further examine this phenotype in a cell autonomous system, we chose the embryonic mesenchymal multipotential progenitor cells C3H10T1/2.
T6007	27288-27290	TO	denotes	To
T6009	27291-27298	RB	denotes	further
T6008	27299-27306	VB	denotes	examine
T6011	27307-27311	DT	denotes	this
T6012	27312-27321	NN	denotes	phenotype
T6013	27322-27324	IN	denotes	in
T6014	27325-27326	DT	denotes	a
T6016	27327-27331	NN	denotes	cell
T6017	27332-27342	JJ	denotes	autonomous
T6015	27343-27349	NN	denotes	system
T6018	27349-27351	,	denotes	,
T6019	27351-27353	PRP	denotes	we
T6010	27354-27359	VBD	denotes	chose
T6020	27360-27363	DT	denotes	the
T6022	27364-27373	JJ	denotes	embryonic
T6023	27374-27385	JJ	denotes	mesenchymal
T6024	27386-27400	JJ	denotes	multipotential
T6025	27401-27411	NN	denotes	progenitor
T6021	27412-27417	NNS	denotes	cells
T6026	27418-27425	NN	denotes	C3H10T1
T6027	27425-27426	HYPH	denotes	/
T6028	27426-27427	CD	denotes	2
T6029	27427-27428	.	denotes	.
T6030	27428-27587	sentence	denotes	The addition of BMP-2 induced osteoblast differentiation, as evidenced by an approximately 400-fold increase in expression of the osteocalcin (OCN) gene [51].
T6031	27429-27432	DT	denotes	The
T6032	27433-27441	NN	denotes	addition
T6034	27442-27444	IN	denotes	of
T6035	27445-27448	NN	denotes	BMP
T6036	27448-27449	HYPH	denotes	-
T6037	27449-27450	CD	denotes	2
T6033	27451-27458	VBD	denotes	induced
T6038	27459-27469	NN	denotes	osteoblast
T6039	27470-27485	NN	denotes	differentiation
T6040	27485-27487	,	denotes	,
T6041	27487-27489	IN	denotes	as
T6042	27490-27499	VBN	denotes	evidenced
T6043	27500-27502	IN	denotes	by
T6044	27503-27505	DT	denotes	an
T6046	27506-27519	RB	denotes	approximately
T6047	27520-27528	JJ	denotes	400-fold
T6045	27529-27537	NN	denotes	increase
T6048	27538-27540	IN	denotes	in
T6049	27541-27551	NN	denotes	expression
T6050	27552-27554	IN	denotes	of
T6051	27555-27558	DT	denotes	the
T6053	27559-27570	NN	denotes	osteocalcin
T6054	27571-27572	-LRB-	denotes	(
T6055	27572-27575	NN	denotes	OCN
T6056	27575-27576	-RRB-	denotes	)
T6052	27577-27581	NN	denotes	gene
T6057	27582-27583	-LRB-	denotes	[
T6058	27583-27585	CD	denotes	51
T6059	27585-27586	-RRB-	denotes	]
T6060	27586-27587	.	denotes	.
T6061	27587-27769	sentence	denotes	Populations of C3H10T1/2 cells stably transfected with either pSuper-retro (control) and pSuper-retro harboring a short hairpin against Sam68 (Sam68sh) were selected with puromycin.
T6062	27588-27599	NNS	denotes	Populations
T6064	27600-27602	IN	denotes	of
T6065	27603-27610	NN	denotes	C3H10T1
T6067	27610-27611	HYPH	denotes	/
T6068	27611-27612	CD	denotes	2
T6066	27613-27618	NNS	denotes	cells
T6069	27619-27625	RB	denotes	stably
T6070	27626-27637	VBN	denotes	transfected
T6071	27638-27642	IN	denotes	with
T6072	27643-27649	CC	denotes	either
T6074	27650-27656	NN	denotes	pSuper
T6075	27656-27657	HYPH	denotes	-
T6073	27657-27662	NN	denotes	retro
T6076	27663-27664	-LRB-	denotes	(
T6077	27664-27671	NN	denotes	control
T6078	27671-27672	-RRB-	denotes	)
T6079	27673-27676	CC	denotes	and
T6080	27677-27683	NN	denotes	pSuper
T6082	27683-27684	HYPH	denotes	-
T6081	27684-27689	NN	denotes	retro
T6083	27690-27699	VBG	denotes	harboring
T6084	27700-27701	DT	denotes	a
T6086	27702-27707	JJ	denotes	short
T6085	27708-27715	NN	denotes	hairpin
T6087	27716-27723	IN	denotes	against
T6088	27724-27729	NN	denotes	Sam68
T6089	27730-27731	-LRB-	denotes	(
T6090	27731-27738	NN	denotes	Sam68sh
T6091	27738-27739	-RRB-	denotes	)
T6092	27740-27744	VBD	denotes	were
T6063	27745-27753	VBN	denotes	selected
T6093	27754-27758	IN	denotes	with
T6094	27759-27768	NN	denotes	puromycin
T6095	27768-27769	.	denotes	.
T6096	27769-27910	sentence	denotes	The expression of Sam68 was reduced by approximately 80% as evidenced by immunoblot analyses using β-actin as a loading control (Figure 8A).
T6097	27770-27773	DT	denotes	The
T6098	27774-27784	NN	denotes	expression
T6100	27785-27787	IN	denotes	of
T6101	27788-27793	NN	denotes	Sam68
T6102	27794-27797	VBD	denotes	was
T6099	27798-27805	VBN	denotes	reduced
T6103	27806-27808	IN	denotes	by
T6104	27809-27822	RB	denotes	approximately
T6105	27823-27825	CD	denotes	80
T6106	27825-27826	NN	denotes	%
T6107	27827-27829	IN	denotes	as
T6108	27830-27839	VBN	denotes	evidenced
T6109	27840-27842	IN	denotes	by
T6110	27843-27853	NN	denotes	immunoblot
T6111	27854-27862	NNS	denotes	analyses
T6112	27863-27868	VBG	denotes	using
T6113	27869-27870	NN	denotes	β
T6115	27870-27871	HYPH	denotes	-
T6114	27871-27876	NN	denotes	actin
T6116	27877-27879	IN	denotes	as
T6117	27880-27881	DT	denotes	a
T6119	27882-27889	NN	denotes	loading
T6118	27890-27897	NN	denotes	control
T6120	27898-27899	-LRB-	denotes	(
T6122	27899-27905	NN	denotes	Figure
T6121	27906-27908	NN	denotes	8A
T6123	27908-27909	-RRB-	denotes	)
T6124	27909-27910	.	denotes	.
T6125	27910-28055	sentence	denotes	Osteoblast differentiation was induced in cultures expressing pSuper-retro or pSuper-retro Sam68sh by addition of BMP-2 to the culture medium. .
T6126	27911-27921	NN	denotes	Osteoblast
T6127	27922-27937	NN	denotes	differentiation
T6129	27938-27941	VBD	denotes	was
T6128	27942-27949	VBN	denotes	induced
T6130	27950-27952	IN	denotes	in
T6131	27953-27961	NNS	denotes	cultures
T6132	27962-27972	VBG	denotes	expressing
T6133	27973-27979	NN	denotes	pSuper
T6135	27979-27980	HYPH	denotes	-
T6134	27980-27985	NN	denotes	retro
T6136	27986-27988	CC	denotes	or
T6137	27989-27995	NN	denotes	pSuper
T6139	27995-27996	HYPH	denotes	-
T6138	27996-28001	NN	denotes	retro
T6140	28002-28009	NN	denotes	Sam68sh
T6141	28010-28012	IN	denotes	by
T6142	28013-28021	NN	denotes	addition
T6143	28022-28024	IN	denotes	of
T6144	28025-28028	NN	denotes	BMP
T6145	28028-28029	HYPH	denotes	-
T6146	28029-28030	CD	denotes	2
T6147	28031-28033	IN	denotes	to
T6148	28034-28037	DT	denotes	the
T6150	28038-28045	NN	denotes	culture
T6149	28046-28052	NN	denotes	medium
T6151	28052-28053	.	denotes	.
T6152	28054-28055	.	denotes	.
T6153	28055-28296	sentence	denotes	The expression of OCN and β-actin mRNAs was examined by semiquantitative RT-PCR and the Sam68sh-expressing cells displayed a more pronounced osteoblast phenotype compared with control cells, as assessed by the expression of OCN (Figure 8B).
T6154	28056-28059	DT	denotes	The
T6155	28060-28070	NN	denotes	expression
T6157	28071-28073	IN	denotes	of
T6158	28074-28077	NN	denotes	OCN
T6159	28078-28081	CC	denotes	and
T6160	28082-28083	NN	denotes	β
T6162	28083-28084	HYPH	denotes	-
T6161	28084-28089	NN	denotes	actin
T6163	28090-28095	NNS	denotes	mRNAs
T6164	28096-28099	VBD	denotes	was
T6156	28100-28108	VBN	denotes	examined
T6165	28109-28111	IN	denotes	by
T6166	28112-28128	JJ	denotes	semiquantitative
T6168	28129-28131	NN	denotes	RT
T6169	28131-28132	HYPH	denotes	-
T6167	28132-28135	NN	denotes	PCR
T6170	28136-28139	CC	denotes	and
T6171	28140-28143	DT	denotes	the
T6173	28144-28151	NN	denotes	Sam68sh
T6175	28151-28152	HYPH	denotes	-
T6174	28152-28162	VBG	denotes	expressing
T6172	28163-28168	NNS	denotes	cells
T6176	28169-28178	VBD	denotes	displayed
T6177	28179-28180	DT	denotes	a
T6179	28181-28185	RBR	denotes	more
T6180	28186-28196	JJ	denotes	pronounced
T6181	28197-28207	NN	denotes	osteoblast
T6178	28208-28217	NN	denotes	phenotype
T6182	28218-28226	VBN	denotes	compared
T6183	28227-28231	IN	denotes	with
T6184	28232-28239	NN	denotes	control
T6185	28240-28245	NNS	denotes	cells
T6186	28245-28247	,	denotes	,
T6187	28247-28249	IN	denotes	as
T6188	28250-28258	VBN	denotes	assessed
T6189	28259-28261	IN	denotes	by
T6190	28262-28265	DT	denotes	the
T6191	28266-28276	NN	denotes	expression
T6192	28277-28279	IN	denotes	of
T6193	28280-28283	NN	denotes	OCN
T6194	28284-28285	-LRB-	denotes	(
T6196	28285-28291	NN	denotes	Figure
T6195	28292-28294	NN	denotes	8B
T6197	28294-28295	-RRB-	denotes	)
T6198	28295-28296	.	denotes	.
T6199	28296-29429	sentence	denotes	Figure 8 Enhanced Osteogenic Differentiation of the C3HT101/2 Embryonic Cell Line Depleted of Endogenous Sam68 (A) C3HT101/2 cells transfected with an empty vector (pSuper-retro) or a vector containing an shRNA (Sam68 shRNA) were selected with puromycin, and knockdown populations depleted of Sam68 were identified. The reduction in Sam68 protein was analyzed by immunoblotting with anti-Sam68 (AD1) antibody and anti–β-actin antibodies as loading controls. (B) Osteogenic differentiation was carried out with conditioned medium containing BMP-2 for the indicated times. To assess the level of osteogenic differentiation in these cells, expression of late osteoblast marker, osteocalcin (OCN), was analyzed by RT-PCR and compared with β-actin and GAPDH controls. The DNA fragments were visualized by agarose gel stained with ethidium bromide. Given the apparent enhancement of mineralized nodule formation by Sam68−/− bone marrow stromal cells ex vivo and the phenotype observed with short hairpin RNA (shRNA)-treated C3H10T1/2, we stained sections of bone from 4- and 12-month-old mice for evidence of changes in marrow adiposity.
T6200	29141-29146	VBN	denotes	Given
T6202	29147-29150	DT	denotes	the
T6204	29151-29159	JJ	denotes	apparent
T6203	29160-29171	NN	denotes	enhancement
T6205	29172-29174	IN	denotes	of
T6206	29175-29186	VBN	denotes	mineralized
T6207	29187-29193	NN	denotes	nodule
T6208	29194-29203	NN	denotes	formation
T6209	29204-29206	IN	denotes	by
T6210	29207-29212	NN	denotes	Sam68
T6212	29212-29213	SYM	denotes	−
T6213	29213-29214	HYPH	denotes	/
T6214	29214-29215	SYM	denotes	−
T6215	29216-29220	NN	denotes	bone
T6216	29221-29227	NN	denotes	marrow
T6217	29228-29235	JJ	denotes	stromal
T6211	29236-29241	NNS	denotes	cells
T6218	29242-29244	FW	denotes	ex
T6219	29245-29249	FW	denotes	vivo
T6220	29250-29253	CC	denotes	and
T6221	29254-29257	DT	denotes	the
T6222	29258-29267	NN	denotes	phenotype
T6223	29268-29276	VBN	denotes	observed
T6224	29277-29281	IN	denotes	with
T6225	29282-29287	JJ	denotes	short
T6227	29288-29295	NN	denotes	hairpin
T6226	29296-29299	NN	denotes	RNA
T6229	29300-29301	-LRB-	denotes	(
T6230	29301-29306	NN	denotes	shRNA
T6231	29306-29307	-RRB-	denotes	)
T6232	29307-29308	HYPH	denotes	-
T6228	29308-29315	VBN	denotes	treated
T6233	29316-29323	NN	denotes	C3H10T1
T6234	29323-29324	HYPH	denotes	/
T6235	29324-29325	CD	denotes	2
T6236	29325-29327	,	denotes	,
T6237	29327-29329	PRP	denotes	we
T6201	29330-29337	VBD	denotes	stained
T6238	29338-29346	NNS	denotes	sections
T6239	29347-29349	IN	denotes	of
T6240	29350-29354	NN	denotes	bone
T6241	29355-29359	IN	denotes	from
T6242	29360-29361	CD	denotes	4
T6244	29361-29362	HYPH	denotes	-
T6245	29363-29366	CC	denotes	and
T6246	29367-29369	CD	denotes	12
T6247	29369-29370	HYPH	denotes	-
T6243	29370-29375	NN	denotes	month
T6249	29375-29376	HYPH	denotes	-
T6248	29376-29379	JJ	denotes	old
T6250	29380-29384	NNS	denotes	mice
T6251	29385-29388	IN	denotes	for
T6252	29389-29397	NN	denotes	evidence
T6253	29398-29400	IN	denotes	of
T6254	29401-29408	NNS	denotes	changes
T6255	29409-29411	IN	denotes	in
T6256	29412-29418	NN	denotes	marrow
T6257	29419-29428	NN	denotes	adiposity
T6258	29428-29429	.	denotes	.
T6259	29429-29747	sentence	denotes	Figure 9 shows sections of undecalcified bone from 4- and 12-month-old Sam68+/+ and Sam68−/− mice stained with von Kossa and toluidine blue to show mineralized tissue (Figure 9A, black) and marrow adipocytes (Figure 9B, white) and left unstained to show fluorochrome labeling of the mineralization fronts (Figure 9C).
T6260	29430-29436	NN	denotes	Figure
T6262	29437-29438	CD	denotes	9
T6261	29439-29444	VBZ	denotes	shows
T6263	29445-29453	NNS	denotes	sections
T6264	29454-29456	IN	denotes	of
T6265	29457-29470	JJ	denotes	undecalcified
T6266	29471-29475	NN	denotes	bone
T6267	29476-29480	IN	denotes	from
T6268	29481-29482	CD	denotes	4
T6270	29482-29483	HYPH	denotes	-
T6271	29484-29487	CC	denotes	and
T6272	29488-29490	CD	denotes	12
T6273	29490-29491	HYPH	denotes	-
T6269	29491-29496	NN	denotes	month
T6275	29496-29497	HYPH	denotes	-
T6274	29497-29500	JJ	denotes	old
T6277	29501-29506	NN	denotes	Sam68
T6278	29506-29507	SYM	denotes	+
T6279	29507-29508	HYPH	denotes	/
T6280	29508-29509	SYM	denotes	+
T6281	29510-29513	CC	denotes	and
T6282	29514-29519	NN	denotes	Sam68
T6283	29519-29520	SYM	denotes	−
T6284	29520-29521	HYPH	denotes	/
T6285	29521-29522	SYM	denotes	−
T6276	29523-29527	NNS	denotes	mice
T6286	29528-29535	VBN	denotes	stained
T6287	29536-29540	IN	denotes	with
T6288	29541-29544	NNP	denotes	von
T6289	29545-29550	NNP	denotes	Kossa
T6290	29551-29554	CC	denotes	and
T6291	29555-29564	NN	denotes	toluidine
T6292	29565-29569	JJ	denotes	blue
T6293	29570-29572	TO	denotes	to
T6294	29573-29577	VB	denotes	show
T6295	29578-29589	VBN	denotes	mineralized
T6296	29590-29596	NN	denotes	tissue
T6297	29597-29598	-LRB-	denotes	(
T6299	29598-29604	NN	denotes	Figure
T6298	29605-29607	NN	denotes	9A
T6300	29607-29609	,	denotes	,
T6301	29609-29614	JJ	denotes	black
T6302	29614-29615	-RRB-	denotes	)
T6303	29616-29619	CC	denotes	and
T6304	29620-29626	NN	denotes	marrow
T6305	29627-29637	NNS	denotes	adipocytes
T6306	29638-29639	-LRB-	denotes	(
T6308	29639-29645	NN	denotes	Figure
T6307	29646-29648	NN	denotes	9B
T6309	29648-29650	,	denotes	,
T6310	29650-29655	JJ	denotes	white
T6311	29655-29656	-RRB-	denotes	)
T6312	29657-29660	CC	denotes	and
T6313	29661-29665	VBD	denotes	left
T6314	29666-29675	JJ	denotes	unstained
T6315	29676-29678	TO	denotes	to
T6316	29679-29683	VB	denotes	show
T6317	29684-29696	NN	denotes	fluorochrome
T6318	29697-29705	NN	denotes	labeling
T6319	29706-29708	IN	denotes	of
T6320	29709-29712	DT	denotes	the
T6322	29713-29727	NN	denotes	mineralization
T6321	29728-29734	NNS	denotes	fronts
T6323	29735-29736	-LRB-	denotes	(
T6325	29736-29742	NN	denotes	Figure
T6324	29743-29745	NN	denotes	9C
T6326	29745-29746	-RRB-	denotes	)
T6327	29746-29747	.	denotes	.
T6328	29747-30000	sentence	denotes	Twelve-month-old Sam68+/+ mice showed a noticeable decrease in trabecular bone (Figure 9A), which was associated with a significant increase in marrow adiposity (Figure 9B) and with the two fluorochrome labels superimposed upon one another (Figure 9C).
T6329	29748-29754	CD	denotes	Twelve
T6331	29754-29755	HYPH	denotes	-
T6330	29755-29760	NN	denotes	month
T6333	29760-29761	HYPH	denotes	-
T6332	29761-29764	JJ	denotes	old
T6335	29765-29770	NN	denotes	Sam68
T6336	29770-29771	SYM	denotes	+
T6337	29771-29772	HYPH	denotes	/
T6338	29772-29773	SYM	denotes	+
T6334	29774-29778	NNS	denotes	mice
T6339	29779-29785	VBD	denotes	showed
T6340	29786-29787	DT	denotes	a
T6342	29788-29798	JJ	denotes	noticeable
T6341	29799-29807	NN	denotes	decrease
T6343	29808-29810	IN	denotes	in
T6344	29811-29821	JJ	denotes	trabecular
T6345	29822-29826	NN	denotes	bone
T6346	29827-29828	-LRB-	denotes	(
T6348	29828-29834	NN	denotes	Figure
T6347	29835-29837	NN	denotes	9A
T6349	29837-29838	-RRB-	denotes	)
T6350	29838-29840	,	denotes	,
T6351	29840-29845	WDT	denotes	which
T6353	29846-29849	VBD	denotes	was
T6352	29850-29860	VBN	denotes	associated
T6354	29861-29865	IN	denotes	with
T6355	29866-29867	DT	denotes	a
T6357	29868-29879	JJ	denotes	significant
T6356	29880-29888	NN	denotes	increase
T6358	29889-29891	IN	denotes	in
T6359	29892-29898	NN	denotes	marrow
T6360	29899-29908	NN	denotes	adiposity
T6361	29909-29910	-LRB-	denotes	(
T6363	29910-29916	NN	denotes	Figure
T6362	29917-29919	NN	denotes	9B
T6364	29919-29920	-RRB-	denotes	)
T6365	29921-29924	CC	denotes	and
T6366	29925-29929	IN	denotes	with
T6367	29930-29933	DT	denotes	the
T6369	29934-29937	CD	denotes	two
T6370	29938-29950	NN	denotes	fluorochrome
T6368	29951-29957	NNS	denotes	labels
T6371	29958-29970	VBN	denotes	superimposed
T6372	29971-29975	IN	denotes	upon
T6373	29976-29979	CD	denotes	one
T6374	29980-29987	DT	denotes	another
T6375	29988-29989	-LRB-	denotes	(
T6377	29989-29995	NN	denotes	Figure
T6376	29996-29998	NN	denotes	9C
T6378	29998-29999	-RRB-	denotes	)
T6379	29999-30000	.	denotes	.
T6380	30000-30123	sentence	denotes	In contrast, the bones of 12-month-old Sam68−/− mice appeared similar to those of the 4-month-old mice of either genotype.
T6381	30001-30003	IN	denotes	In
T6383	30004-30012	NN	denotes	contrast
T6384	30012-30014	,	denotes	,
T6385	30014-30017	DT	denotes	the
T6386	30018-30023	NNS	denotes	bones
T6387	30024-30026	IN	denotes	of
T6388	30027-30029	CD	denotes	12
T6390	30029-30030	HYPH	denotes	-
T6389	30030-30035	NN	denotes	month
T6392	30035-30036	HYPH	denotes	-
T6391	30036-30039	JJ	denotes	old
T6394	30040-30045	NN	denotes	Sam68
T6395	30045-30046	SYM	denotes	−
T6396	30046-30047	HYPH	denotes	/
T6397	30047-30048	SYM	denotes	−
T6393	30049-30053	NNS	denotes	mice
T6382	30054-30062	VBD	denotes	appeared
T6398	30063-30070	JJ	denotes	similar
T6399	30071-30073	IN	denotes	to
T6400	30074-30079	DT	denotes	those
T6401	30080-30082	IN	denotes	of
T6402	30083-30086	DT	denotes	the
T6404	30087-30088	CD	denotes	4
T6406	30088-30089	HYPH	denotes	-
T6405	30089-30094	NN	denotes	month
T6408	30094-30095	HYPH	denotes	-
T6407	30095-30098	JJ	denotes	old
T6403	30099-30103	NNS	denotes	mice
T6409	30104-30106	IN	denotes	of
T6410	30107-30113	DT	denotes	either
T6411	30114-30122	NN	denotes	genotype
T6412	30122-30123	.	denotes	.
T6413	30123-30311	sentence	denotes	These data demonstrate that Sam68 regulates the differentiation of bone marrow mesenchymal cells to promote adipocyte differentiation and inhibit osteoblast differentiation in aging bone.
T6414	30124-30129	DT	denotes	These
T6415	30130-30134	NNS	denotes	data
T6416	30135-30146	VBP	denotes	demonstrate
T6417	30147-30151	IN	denotes	that
T6419	30152-30157	NN	denotes	Sam68
T6418	30158-30167	VBZ	denotes	regulates
T6420	30168-30171	DT	denotes	the
T6421	30172-30187	NN	denotes	differentiation
T6422	30188-30190	IN	denotes	of
T6423	30191-30195	NN	denotes	bone
T6424	30196-30202	NN	denotes	marrow
T6426	30203-30214	JJ	denotes	mesenchymal
T6425	30215-30220	NNS	denotes	cells
T6427	30221-30223	TO	denotes	to
T6428	30224-30231	VB	denotes	promote
T6429	30232-30241	NN	denotes	adipocyte
T6430	30242-30257	NN	denotes	differentiation
T6431	30258-30261	CC	denotes	and
T6432	30262-30269	VB	denotes	inhibit
T6433	30270-30280	NN	denotes	osteoblast
T6434	30281-30296	NN	denotes	differentiation
T6435	30297-30299	IN	denotes	in
T6436	30300-30305	VBG	denotes	aging
T6437	30306-30310	NN	denotes	bone
T6438	30310-30311	.	denotes	.
T7333	30996-30999	DT	denotes	The
T7335	31000-31007	JJ	denotes	present
T7334	31008-31013	NN	denotes	study
T7336	31014-31022	VBZ	denotes	provides
T7337	31023-31031	NN	denotes	evidence
T7338	31032-31036	IN	denotes	that
T7340	31037-31038	DT	denotes	a
T7342	31039-31050	JJ	denotes	physiologic
T7341	31051-31055	NN	denotes	role
T7343	31056-31058	IN	denotes	of
T7344	31059-31064	NN	denotes	Sam68
T7339	31065-31067	VBZ	denotes	is
T7345	31068-31070	TO	denotes	to
T7346	31071-31079	VB	denotes	modulate
T7347	31080-31084	NN	denotes	bone
T7348	31085-31091	NN	denotes	marrow
T7350	31092-31103	JJ	denotes	mesenchymal
T7351	31104-31108	NN	denotes	stem
T7349	31109-31114	NNS	denotes	cells
T7352	31114-31115	.	denotes	.
T7353	31115-31223	sentence	denotes	Young Sam68−/− mice developed normally and contained similar bone mass compared with wild-type littermates.
T7354	31116-31121	JJ	denotes	Young
T7356	31122-31127	NN	denotes	Sam68
T7357	31127-31128	SYM	denotes	−
T7358	31128-31129	HYPH	denotes	/
T7359	31129-31130	SYM	denotes	−
T7355	31131-31135	NNS	denotes	mice
T7360	31136-31145	VBD	denotes	developed
T7361	31146-31154	RB	denotes	normally
T7362	31155-31158	CC	denotes	and
T7363	31159-31168	VBD	denotes	contained
T7364	31169-31176	JJ	denotes	similar
T7366	31177-31181	NN	denotes	bone
T7365	31182-31186	NN	denotes	mass
T7367	31187-31195	VBN	denotes	compared
T7368	31196-31200	IN	denotes	with
T7369	31201-31205	JJ	denotes	wild
T7371	31205-31206	HYPH	denotes	-
T7370	31206-31210	NN	denotes	type
T7372	31211-31222	NNS	denotes	littermates
T7373	31222-31223	.	denotes	.
T7374	31223-31327	sentence	denotes	Aged (12 months) Sam68−/− mice displayed a high bone mass phenotype compared with Sam68+/+ littermates.
T7375	31224-31228	VBN	denotes	Aged
T7377	31229-31230	-LRB-	denotes	(
T7379	31230-31232	CD	denotes	12
T7378	31233-31239	NNS	denotes	months
T7380	31239-31240	-RRB-	denotes	)
T7381	31241-31246	NN	denotes	Sam68
T7382	31246-31247	SYM	denotes	−
T7383	31247-31248	HYPH	denotes	/
T7384	31248-31249	SYM	denotes	−
T7376	31250-31254	NNS	denotes	mice
T7385	31255-31264	VBD	denotes	displayed
T7386	31265-31266	DT	denotes	a
T7388	31267-31271	JJ	denotes	high
T7390	31272-31276	NN	denotes	bone
T7389	31277-31281	NN	denotes	mass
T7387	31282-31291	NN	denotes	phenotype
T7391	31292-31300	VBN	denotes	compared
T7392	31301-31305	IN	denotes	with
T7393	31306-31311	NN	denotes	Sam68
T7395	31311-31312	SYM	denotes	+
T7396	31312-31313	HYPH	denotes	/
T7397	31313-31314	SYM	denotes	+
T7394	31315-31326	NNS	denotes	littermates
T7398	31326-31327	.	denotes	.
T7399	31327-31484	sentence	denotes	The wild-type littermates underwent age-related bone loss that occurs naturally in mammals, while the Sam68−/− animals preserved their bone mass with aging.
T7400	31328-31331	DT	denotes	The
T7402	31332-31336	JJ	denotes	wild
T7404	31336-31337	HYPH	denotes	-
T7403	31337-31341	NN	denotes	type
T7401	31342-31353	NNS	denotes	littermates
T7405	31354-31363	VBD	denotes	underwent
T7406	31364-31367	NN	denotes	age
T7408	31367-31368	HYPH	denotes	-
T7407	31368-31375	VBN	denotes	related
T7410	31376-31380	NN	denotes	bone
T7409	31381-31385	NN	denotes	loss
T7411	31386-31390	WDT	denotes	that
T7412	31391-31397	VBZ	denotes	occurs
T7413	31398-31407	RB	denotes	naturally
T7414	31408-31410	IN	denotes	in
T7415	31411-31418	NNS	denotes	mammals
T7416	31418-31420	,	denotes	,
T7417	31420-31425	IN	denotes	while
T7419	31426-31429	DT	denotes	the
T7421	31430-31435	NN	denotes	Sam68
T7422	31435-31436	SYM	denotes	−
T7423	31436-31437	HYPH	denotes	/
T7424	31437-31438	SYM	denotes	−
T7420	31439-31446	NNS	denotes	animals
T7418	31447-31456	VBD	denotes	preserved
T7425	31457-31462	PRP$	denotes	their
T7427	31463-31467	NN	denotes	bone
T7426	31468-31472	NN	denotes	mass
T7428	31473-31477	IN	denotes	with
T7429	31478-31483	NN	denotes	aging
T7430	31483-31484	.	denotes	.
T7431	31484-31709	sentence	denotes	The differentiation of bone marrow stem cells isolated from Sam68−/− mice and embryonic mesenchymal multipotential progenitor cells C3H10T1/2 treated with Sam68 shRNA resulted in a more pronounced osteoblast differentiation.
T7432	31485-31488	DT	denotes	The
T7433	31489-31504	NN	denotes	differentiation
T7435	31505-31507	IN	denotes	of
T7436	31508-31512	NN	denotes	bone
T7437	31513-31519	NN	denotes	marrow
T7439	31520-31524	NN	denotes	stem
T7438	31525-31530	NNS	denotes	cells
T7440	31531-31539	VBN	denotes	isolated
T7441	31540-31544	IN	denotes	from
T7442	31545-31550	NN	denotes	Sam68
T7444	31550-31551	SYM	denotes	−
T7445	31551-31552	HYPH	denotes	/
T7446	31552-31553	SYM	denotes	−
T7443	31554-31558	NNS	denotes	mice
T7447	31559-31562	CC	denotes	and
T7448	31563-31572	JJ	denotes	embryonic
T7450	31573-31584	JJ	denotes	mesenchymal
T7451	31585-31599	JJ	denotes	multipotential
T7452	31600-31610	NN	denotes	progenitor
T7449	31611-31616	NNS	denotes	cells
T7453	31617-31624	NN	denotes	C3H10T1
T7454	31624-31625	HYPH	denotes	/
T7455	31625-31626	CD	denotes	2
T7456	31627-31634	VBN	denotes	treated
T7457	31635-31639	IN	denotes	with
T7458	31640-31645	NN	denotes	Sam68
T7459	31646-31651	NN	denotes	shRNA
T7434	31652-31660	VBD	denotes	resulted
T7460	31661-31663	IN	denotes	in
T7461	31664-31665	DT	denotes	a
T7463	31666-31670	RBR	denotes	more
T7464	31671-31681	JJ	denotes	pronounced
T7465	31682-31692	NN	denotes	osteoblast
T7462	31693-31708	NN	denotes	differentiation
T7466	31708-31709	.	denotes	.
T7467	31709-31796	sentence	denotes	These findings demonstrate that the loss of Sam68 enhances osteoblast differentiation.
T7468	31710-31715	DT	denotes	These
T7469	31716-31724	NNS	denotes	findings
T7470	31725-31736	VBP	denotes	demonstrate
T7471	31737-31741	IN	denotes	that
T7473	31742-31745	DT	denotes	the
T7474	31746-31750	NN	denotes	loss
T7475	31751-31753	IN	denotes	of
T7476	31754-31759	NN	denotes	Sam68
T7472	31760-31768	VBZ	denotes	enhances
T7477	31769-31779	NN	denotes	osteoblast
T7478	31780-31795	NN	denotes	differentiation
T7479	31795-31796	.	denotes	.
T7480	31796-31960	sentence	denotes	The converse was also true, as MEFs isolated from Sam68−/− animals were impaired in their ability to undergo adipocyte differentiation compared with Sam68+/+ MEFs.
T7481	31797-31800	DT	denotes	The
T7482	31801-31809	NN	denotes	converse
T7483	31810-31813	VBD	denotes	was
T7484	31814-31818	RB	denotes	also
T7485	31819-31823	JJ	denotes	true
T7486	31823-31825	,	denotes	,
T7487	31825-31827	IN	denotes	as
T7489	31828-31832	NNS	denotes	MEFs
T7490	31833-31841	VBN	denotes	isolated
T7491	31842-31846	IN	denotes	from
T7492	31847-31852	NN	denotes	Sam68
T7494	31852-31853	SYM	denotes	−
T7495	31853-31854	HYPH	denotes	/
T7496	31854-31855	SYM	denotes	−
T7493	31856-31863	NNS	denotes	animals
T7497	31864-31868	VBD	denotes	were
T7488	31869-31877	VBN	denotes	impaired
T7498	31878-31880	IN	denotes	in
T7499	31881-31886	PRP$	denotes	their
T7500	31887-31894	NN	denotes	ability
T7501	31895-31897	TO	denotes	to
T7502	31898-31905	VB	denotes	undergo
T7503	31906-31915	NN	denotes	adipocyte
T7504	31916-31931	NN	denotes	differentiation
T7505	31932-31940	VBN	denotes	compared
T7506	31941-31945	IN	denotes	with
T7507	31946-31951	NN	denotes	Sam68
T7509	31951-31952	SYM	denotes	+
T7510	31952-31953	HYPH	denotes	/
T7511	31953-31954	SYM	denotes	+
T7508	31955-31959	NNS	denotes	MEFs
T7512	31959-31960	.	denotes	.
T7513	31960-32126	sentence	denotes	These findings suggest that a physiologic role for Sam68 is to regulate the balance between adipogenic and osteogenic differentiation of the bone marrow mesenchymal.
T7514	31961-31966	DT	denotes	These
T7515	31967-31975	NNS	denotes	findings
T7516	31976-31983	VBP	denotes	suggest
T7517	31984-31988	IN	denotes	that
T7519	31989-31990	DT	denotes	a
T7521	31991-32002	JJ	denotes	physiologic
T7520	32003-32007	NN	denotes	role
T7522	32008-32011	IN	denotes	for
T7523	32012-32017	NN	denotes	Sam68
T7518	32018-32020	VBZ	denotes	is
T7524	32021-32023	TO	denotes	to
T7525	32024-32032	VB	denotes	regulate
T7526	32033-32036	DT	denotes	the
T7527	32037-32044	NN	denotes	balance
T7528	32045-32052	IN	denotes	between
T7529	32053-32063	JJ	denotes	adipogenic
T7531	32064-32067	CC	denotes	and
T7532	32068-32078	JJ	denotes	osteogenic
T7530	32079-32094	NN	denotes	differentiation
T7533	32095-32097	IN	denotes	of
T7534	32098-32101	DT	denotes	the
T7536	32102-32106	NN	denotes	bone
T7535	32107-32113	NN	denotes	marrow
T7537	32114-32125	JJ	denotes	mesenchymal
T7538	32125-32126	.	denotes	.
T7539	32126-32297	sentence	denotes	Sam68 protein expression was observed throughout the developing mouse embryo, in keeping with previous reports that identified the Sam68 mRNA to be widely expressed [38].
T7540	32127-32132	NN	denotes	Sam68
T7542	32133-32140	NN	denotes	protein
T7541	32141-32151	NN	denotes	expression
T7544	32152-32155	VBD	denotes	was
T7543	32156-32164	VBN	denotes	observed
T7545	32165-32175	IN	denotes	throughout
T7546	32176-32179	DT	denotes	the
T7548	32180-32190	VBG	denotes	developing
T7549	32191-32196	NN	denotes	mouse
T7547	32197-32203	NN	denotes	embryo
T7550	32203-32205	,	denotes	,
T7551	32205-32207	IN	denotes	in
T7552	32208-32215	VBG	denotes	keeping
T7553	32216-32220	IN	denotes	with
T7554	32221-32229	JJ	denotes	previous
T7555	32230-32237	NNS	denotes	reports
T7556	32238-32242	WDT	denotes	that
T7557	32243-32253	VBD	denotes	identified
T7558	32254-32257	DT	denotes	the
T7560	32258-32263	NN	denotes	Sam68
T7559	32264-32268	NN	denotes	mRNA
T7562	32269-32271	TO	denotes	to
T7563	32272-32274	VB	denotes	be
T7564	32275-32281	RB	denotes	widely
T7561	32282-32291	VBN	denotes	expressed
T7565	32292-32293	-LRB-	denotes	[
T7566	32293-32295	CD	denotes	38
T7567	32295-32296	-RRB-	denotes	]
T7568	32296-32297	.	denotes	.
T7569	32297-32450	sentence	denotes	We observe Sam68 staining in the brain, heart, and intestine (see Figure 1) as well as liver, skin, and kidney (unpublished data) of late-stage embryos.
T7570	32298-32300	PRP	denotes	We
T7571	32301-32308	VBP	denotes	observe
T7572	32309-32314	NN	denotes	Sam68
T7573	32315-32323	NN	denotes	staining
T7574	32324-32326	IN	denotes	in
T7575	32327-32330	DT	denotes	the
T7576	32331-32336	NN	denotes	brain
T7577	32336-32338	,	denotes	,
T7578	32338-32343	NN	denotes	heart
T7579	32343-32345	,	denotes	,
T7580	32345-32348	CC	denotes	and
T7581	32349-32358	NN	denotes	intestine
T7582	32359-32360	-LRB-	denotes	(
T7583	32360-32363	VB	denotes	see
T7584	32364-32370	NN	denotes	Figure
T7585	32371-32372	CD	denotes	1
T7586	32372-32373	-RRB-	denotes	)
T7587	32374-32376	RB	denotes	as
T7589	32377-32381	RB	denotes	well
T7588	32382-32384	IN	denotes	as
T7590	32385-32390	NN	denotes	liver
T7591	32390-32392	,	denotes	,
T7592	32392-32396	NN	denotes	skin
T7593	32396-32398	,	denotes	,
T7594	32398-32401	CC	denotes	and
T7595	32402-32408	NN	denotes	kidney
T7596	32409-32410	-LRB-	denotes	(
T7598	32410-32421	JJ	denotes	unpublished
T7597	32422-32426	NNS	denotes	data
T7599	32426-32427	-RRB-	denotes	)
T7600	32428-32430	IN	denotes	of
T7601	32431-32435	JJ	denotes	late
T7603	32435-32436	HYPH	denotes	-
T7602	32436-32441	NN	denotes	stage
T7604	32442-32449	NNS	denotes	embryos
T7605	32449-32450	.	denotes	.
T7606	32450-32614	sentence	denotes	This expression pattern is consistent with previous work that has observed Sam68 in neurons [52] and as a substrate of the intestinal SIK/breast tumor kinase [43].
T7607	32451-32455	DT	denotes	This
T7609	32456-32466	NN	denotes	expression
T7608	32467-32474	NN	denotes	pattern
T7610	32475-32477	VBZ	denotes	is
T7611	32478-32488	JJ	denotes	consistent
T7612	32489-32493	IN	denotes	with
T7613	32494-32502	JJ	denotes	previous
T7614	32503-32507	NN	denotes	work
T7615	32508-32512	WDT	denotes	that
T7617	32513-32516	VBZ	denotes	has
T7616	32517-32525	VBN	denotes	observed
T7618	32526-32531	NN	denotes	Sam68
T7619	32532-32534	IN	denotes	in
T7620	32535-32542	NNS	denotes	neurons
T7621	32543-32544	-LRB-	denotes	[
T7622	32544-32546	CD	denotes	52
T7623	32546-32547	-RRB-	denotes	]
T7624	32548-32551	CC	denotes	and
T7625	32552-32554	IN	denotes	as
T7626	32555-32556	DT	denotes	a
T7627	32557-32566	NN	denotes	substrate
T7628	32567-32569	IN	denotes	of
T7629	32570-32573	DT	denotes	the
T7631	32574-32584	JJ	denotes	intestinal
T7632	32585-32588	NN	denotes	SIK
T7633	32588-32589	HYPH	denotes	/
T7634	32589-32595	NN	denotes	breast
T7635	32596-32601	NN	denotes	tumor
T7630	32602-32608	NN	denotes	kinase
T7636	32609-32610	-LRB-	denotes	[
T7637	32610-32612	CD	denotes	43
T7638	32612-32613	-RRB-	denotes	]
T7639	32613-32614	.	denotes	.
T7640	32614-32740	sentence	denotes	The expression of Sam68 was not altered in aging bone marrow stromal cells or in senescencing WI-38 cells (unpublished data).
T7641	32615-32618	DT	denotes	The
T7642	32619-32629	NN	denotes	expression
T7644	32630-32632	IN	denotes	of
T7645	32633-32638	NN	denotes	Sam68
T7646	32639-32642	VBD	denotes	was
T7647	32643-32646	RB	denotes	not
T7643	32647-32654	VBN	denotes	altered
T7648	32655-32657	IN	denotes	in
T7649	32658-32663	VBG	denotes	aging
T7651	32664-32668	NN	denotes	bone
T7652	32669-32675	NN	denotes	marrow
T7653	32676-32683	JJ	denotes	stromal
T7650	32684-32689	NNS	denotes	cells
T7654	32690-32692	CC	denotes	or
T7655	32693-32695	IN	denotes	in
T7656	32696-32708	VBG	denotes	senescencing
T7658	32709-32711	NN	denotes	WI
T7659	32711-32712	HYPH	denotes	-
T7660	32712-32714	CD	denotes	38
T7657	32715-32720	NNS	denotes	cells
T7661	32721-32722	-LRB-	denotes	(
T7663	32722-32733	JJ	denotes	unpublished
T7662	32734-32738	NNS	denotes	data
T7664	32738-32739	-RRB-	denotes	)
T7665	32739-32740	.	denotes	.
T7666	32740-32906	sentence	denotes	The presence of Sam68 in chondrocytes, osteoblasts and osteoclasts in developing cartilage and bone predicted a pivotal role for the protein in skeletal development.
T7667	32741-32744	DT	denotes	The
T7668	32745-32753	NN	denotes	presence
T7670	32754-32756	IN	denotes	of
T7671	32757-32762	NN	denotes	Sam68
T7672	32763-32765	IN	denotes	in
T7673	32766-32778	NNS	denotes	chondrocytes
T7674	32778-32780	,	denotes	,
T7675	32780-32791	NNS	denotes	osteoblasts
T7676	32792-32795	CC	denotes	and
T7677	32796-32807	NNS	denotes	osteoclasts
T7678	32808-32810	IN	denotes	in
T7679	32811-32821	VBG	denotes	developing
T7680	32822-32831	NN	denotes	cartilage
T7681	32832-32835	CC	denotes	and
T7682	32836-32840	NN	denotes	bone
T7669	32841-32850	VBD	denotes	predicted
T7683	32851-32852	DT	denotes	a
T7685	32853-32860	JJ	denotes	pivotal
T7684	32861-32865	NN	denotes	role
T7686	32866-32869	IN	denotes	for
T7687	32870-32873	DT	denotes	the
T7688	32874-32881	NN	denotes	protein
T7689	32882-32884	IN	denotes	in
T7690	32885-32893	JJ	denotes	skeletal
T7691	32894-32905	NN	denotes	development
T7692	32905-32906	.	denotes	.
T7693	32906-33118	sentence	denotes	The Sam68−/− mice were generated using a traditional targeting approach where the functional KH domain was deleted and approximately one third of the Sam68−/− mice survived to adulthood with no apparent defects.
T7694	32907-32910	DT	denotes	The
T7696	32911-32916	NN	denotes	Sam68
T7697	32916-32917	SYM	denotes	−
T7698	32917-32918	HYPH	denotes	/
T7699	32918-32919	SYM	denotes	−
T7695	32920-32924	NNS	denotes	mice
T7701	32925-32929	VBD	denotes	were
T7700	32930-32939	VBN	denotes	generated
T7702	32940-32945	VBG	denotes	using
T7703	32946-32947	DT	denotes	a
T7705	32948-32959	JJ	denotes	traditional
T7706	32960-32969	NN	denotes	targeting
T7704	32970-32978	NN	denotes	approach
T7707	32979-32984	WRB	denotes	where
T7709	32985-32988	DT	denotes	the
T7711	32989-32999	JJ	denotes	functional
T7712	33000-33002	NN	denotes	KH
T7710	33003-33009	NN	denotes	domain
T7713	33010-33013	VBD	denotes	was
T7708	33014-33021	VBN	denotes	deleted
T7714	33022-33025	CC	denotes	and
T7715	33026-33039	RB	denotes	approximately
T7716	33040-33043	CD	denotes	one
T7718	33044-33049	JJ	denotes	third
T7719	33050-33052	IN	denotes	of
T7720	33053-33056	DT	denotes	the
T7722	33057-33062	NN	denotes	Sam68
T7723	33062-33063	SYM	denotes	−
T7724	33063-33064	HYPH	denotes	/
T7725	33064-33065	SYM	denotes	−
T7721	33066-33070	NNS	denotes	mice
T7717	33071-33079	VBD	denotes	survived
T7726	33080-33082	IN	denotes	to
T7727	33083-33092	NN	denotes	adulthood
T7728	33093-33097	IN	denotes	with
T7729	33098-33100	DT	denotes	no
T7731	33101-33109	JJ	denotes	apparent
T7730	33110-33117	NNS	denotes	defects
T7732	33117-33118	.	denotes	.
T7733	33118-33277	sentence	denotes	One explanation for this phenomenon is that Sam68 function is sub-served by one or more of its family members during development such as SLM-1 and SLM-2 [47].
T7734	33119-33122	CD	denotes	One
T7735	33123-33134	NN	denotes	explanation
T7737	33135-33138	IN	denotes	for
T7738	33139-33143	DT	denotes	this
T7739	33144-33154	NN	denotes	phenomenon
T7736	33155-33157	VBZ	denotes	is
T7740	33158-33162	IN	denotes	that
T7742	33163-33168	NN	denotes	Sam68
T7743	33169-33177	NN	denotes	function
T7744	33178-33180	VBZ	denotes	is
T7741	33181-33191	VBN	denotes	sub-served
T7745	33192-33194	IN	denotes	by
T7746	33195-33198	CD	denotes	one
T7747	33199-33201	CC	denotes	or
T7748	33202-33206	JJR	denotes	more
T7749	33207-33209	IN	denotes	of
T7750	33210-33213	PRP$	denotes	its
T7752	33214-33220	NN	denotes	family
T7751	33221-33228	NNS	denotes	members
T7753	33229-33235	IN	denotes	during
T7754	33236-33247	NN	denotes	development
T7755	33248-33252	JJ	denotes	such
T7756	33253-33255	IN	denotes	as
T7757	33256-33259	NN	denotes	SLM
T7758	33259-33260	HYPH	denotes	-
T7759	33260-33261	CD	denotes	1
T7760	33262-33265	CC	denotes	and
T7761	33266-33269	NN	denotes	SLM
T7762	33269-33270	HYPH	denotes	-
T7763	33270-33271	CD	denotes	2
T7764	33272-33273	-LRB-	denotes	[
T7765	33273-33275	CD	denotes	47
T7766	33275-33276	-RRB-	denotes	]
T7767	33276-33277	.	denotes	.
T7768	33277-33344	sentence	denotes	Alternatively, Sam68 is not required during embryonic development.
T7769	33278-33291	RB	denotes	Alternatively
T7771	33291-33293	,	denotes	,
T7772	33293-33298	NN	denotes	Sam68
T7773	33299-33301	VBZ	denotes	is
T7774	33302-33305	RB	denotes	not
T7770	33306-33314	VBN	denotes	required
T7775	33315-33321	IN	denotes	during
T7776	33322-33331	JJ	denotes	embryonic
T7777	33332-33343	NN	denotes	development
T7778	33343-33344	.	denotes	.
T7779	33344-33447	sentence	denotes	However, two thirds of the Sam68−/−, but not the Sam68+/- pups, were killed by their Sam68+/- mothers.
T7780	33345-33352	RB	denotes	However
T7782	33352-33354	,	denotes	,
T7783	33354-33357	CD	denotes	two
T7784	33358-33364	NNS	denotes	thirds
T7785	33365-33367	IN	denotes	of
T7786	33368-33371	DT	denotes	the
T7787	33372-33377	NN	denotes	Sam68
T7788	33377-33378	SYM	denotes	−
T7789	33378-33379	HYPH	denotes	/
T7790	33379-33380	SYM	denotes	−
T7791	33380-33382	,	denotes	,
T7792	33382-33385	CC	denotes	but
T7793	33386-33389	RB	denotes	not
T7794	33390-33393	DT	denotes	the
T7795	33394-33399	NN	denotes	Sam68
T7797	33399-33400	SYM	denotes	+
T7798	33400-33401	HYPH	denotes	/
T7799	33401-33402	SYM	denotes	-
T7796	33403-33407	NNS	denotes	pups
T7800	33407-33409	,	denotes	,
T7801	33409-33413	VBD	denotes	were
T7781	33414-33420	VBN	denotes	killed
T7802	33421-33423	IN	denotes	by
T7803	33424-33429	PRP$	denotes	their
T7805	33430-33435	NN	denotes	Sam68
T7806	33435-33436	SYM	denotes	+
T7807	33436-33437	HYPH	denotes	/
T7808	33437-33438	SYM	denotes	-
T7804	33439-33446	NNS	denotes	mothers
T7809	33446-33447	.	denotes	.
T7810	33447-33549	sentence	denotes	These findings suggest that the mothers are able to detect a subtle defect/difference that we cannot.
T7811	33448-33453	DT	denotes	These
T7812	33454-33462	NNS	denotes	findings
T7813	33463-33470	VBP	denotes	suggest
T7814	33471-33475	IN	denotes	that
T7816	33476-33479	DT	denotes	the
T7817	33480-33487	NNS	denotes	mothers
T7815	33488-33491	VBP	denotes	are
T7818	33492-33496	JJ	denotes	able
T7819	33497-33499	TO	denotes	to
T7820	33500-33506	VB	denotes	detect
T7821	33507-33508	DT	denotes	a
T7823	33509-33515	JJ	denotes	subtle
T7824	33516-33522	NN	denotes	defect
T7825	33522-33523	HYPH	denotes	/
T7822	33523-33533	NN	denotes	difference
T7826	33534-33538	WDT	denotes	that
T7828	33539-33541	PRP	denotes	we
T7827	33542-33545	MD	denotes	can
T7829	33545-33548	RB	denotes	not
T7830	33548-33549	.	denotes	.
T7831	33549-33621	sentence	denotes	Adult Sam68−/− mice live a normal life span of approximately two years.
T7832	33550-33555	JJ	denotes	Adult
T7834	33556-33561	NN	denotes	Sam68
T7835	33561-33562	SYM	denotes	−
T7836	33562-33563	HYPH	denotes	/
T7837	33563-33564	SYM	denotes	−
T7833	33565-33569	NNS	denotes	mice
T7838	33570-33574	VBP	denotes	live
T7839	33575-33576	DT	denotes	a
T7841	33577-33583	JJ	denotes	normal
T7842	33584-33588	NN	denotes	life
T7840	33589-33593	NN	denotes	span
T7843	33594-33596	IN	denotes	of
T7844	33597-33610	RB	denotes	approximately
T7845	33611-33614	CD	denotes	two
T7846	33615-33620	NNS	denotes	years
T7847	33620-33621	.	denotes	.
T7848	33621-33801	sentence	denotes	The Sam68−/− males were sterile and the Sam68−/− females provided inadequate care to their young, but the pups were not scattered and neglected as observed with FosB−/− mice [53].
T7849	33622-33625	DT	denotes	The
T7851	33626-33631	NN	denotes	Sam68
T7852	33631-33632	SYM	denotes	−
T7853	33632-33633	HYPH	denotes	/
T7854	33633-33634	SYM	denotes	−
T7850	33635-33640	NNS	denotes	males
T7855	33641-33645	VBD	denotes	were
T7856	33646-33653	JJ	denotes	sterile
T7857	33654-33657	CC	denotes	and
T7858	33658-33661	DT	denotes	the
T7860	33662-33667	NN	denotes	Sam68
T7861	33667-33668	SYM	denotes	−
T7862	33668-33669	HYPH	denotes	/
T7863	33669-33670	SYM	denotes	−
T7859	33671-33678	NNS	denotes	females
T7864	33679-33687	VBD	denotes	provided
T7865	33688-33698	JJ	denotes	inadequate
T7866	33699-33703	NN	denotes	care
T7867	33704-33706	IN	denotes	to
T7868	33707-33712	PRP$	denotes	their
T7869	33713-33718	JJ	denotes	young
T7870	33718-33720	,	denotes	,
T7871	33720-33723	CC	denotes	but
T7872	33724-33727	DT	denotes	the
T7873	33728-33732	NNS	denotes	pups
T7875	33733-33737	VBD	denotes	were
T7876	33738-33741	RB	denotes	not
T7874	33742-33751	VBN	denotes	scattered
T7877	33752-33755	CC	denotes	and
T7878	33756-33765	VBN	denotes	neglected
T7879	33766-33768	IN	denotes	as
T7880	33769-33777	VBN	denotes	observed
T7881	33778-33782	IN	denotes	with
T7882	33783-33787	NN	denotes	FosB
T7884	33787-33788	SYM	denotes	−
T7885	33788-33789	HYPH	denotes	/
T7886	33789-33790	SYM	denotes	−
T7883	33791-33795	NNS	denotes	mice
T7887	33796-33797	-LRB-	denotes	[
T7888	33797-33799	CD	denotes	53
T7889	33799-33800	-RRB-	denotes	]
T7890	33800-33801	.	denotes	.
T7891	33801-33938	sentence	denotes	Serum levels of estrogen decreased in aging Sam68−/− females as expected; however, the leptin levels decreased in aged Sam68−/− females.
T7892	33802-33807	NN	denotes	Serum
T7893	33808-33814	NNS	denotes	levels
T7895	33815-33817	IN	denotes	of
T7896	33818-33826	NN	denotes	estrogen
T7894	33827-33836	VBD	denotes	decreased
T7898	33837-33839	IN	denotes	in
T7899	33840-33845	VBG	denotes	aging
T7901	33846-33851	NN	denotes	Sam68
T7902	33851-33852	SYM	denotes	−
T7903	33852-33853	HYPH	denotes	/
T7904	33853-33854	SYM	denotes	−
T7900	33855-33862	NNS	denotes	females
T7905	33863-33865	IN	denotes	as
T7906	33866-33874	VBN	denotes	expected
T7907	33874-33875	:	denotes	;
T7908	33876-33883	RB	denotes	however
T7909	33883-33885	,	denotes	,
T7910	33885-33888	DT	denotes	the
T7912	33889-33895	NN	denotes	leptin
T7911	33896-33902	NNS	denotes	levels
T7897	33903-33912	VBD	denotes	decreased
T7913	33913-33915	IN	denotes	in
T7914	33916-33920	JJ	denotes	aged
T7916	33921-33926	NN	denotes	Sam68
T7917	33926-33927	SYM	denotes	−
T7918	33927-33928	HYPH	denotes	/
T7919	33928-33929	SYM	denotes	−
T7915	33930-33937	NNS	denotes	females
T7920	33937-33938	.	denotes	.
T7921	33938-34049	sentence	denotes	The aged Sam68−/− females were not obese and actually weighed less than the littermate controls (see Table 2).
T7922	33939-33942	DT	denotes	The
T7924	33943-33947	JJ	denotes	aged
T7925	33948-33953	NN	denotes	Sam68
T7926	33953-33954	SYM	denotes	−
T7927	33954-33955	HYPH	denotes	/
T7928	33955-33956	SYM	denotes	−
T7923	33957-33964	NNS	denotes	females
T7929	33965-33969	VBD	denotes	were
T7930	33970-33973	RB	denotes	not
T7931	33974-33979	JJ	denotes	obese
T7932	33980-33983	CC	denotes	and
T7933	33984-33992	RB	denotes	actually
T7934	33993-34000	VBD	denotes	weighed
T7935	34001-34005	JJR	denotes	less
T7936	34006-34010	IN	denotes	than
T7937	34011-34014	DT	denotes	the
T7939	34015-34025	NN	denotes	littermate
T7938	34026-34034	NNS	denotes	controls
T7940	34035-34036	-LRB-	denotes	(
T7941	34036-34039	VB	denotes	see
T7942	34040-34045	NN	denotes	Table
T7943	34046-34047	CD	denotes	2
T7944	34047-34048	-RRB-	denotes	)
T7945	34048-34049	.	denotes	.
T7946	34049-34289	sentence	denotes	Moreover, their appetite was not altered with aging (N. Torabi and S. Richard, unpublished data), suggesting that the observed leptin reduction was not recreating ob/ob-like phenotypes related to weight, appetite and female sterility [54].
T7947	34050-34058	RB	denotes	Moreover
T7949	34058-34060	,	denotes	,
T7950	34060-34065	PRP$	denotes	their
T7951	34066-34074	NN	denotes	appetite
T7952	34075-34078	VBD	denotes	was
T7953	34079-34082	RB	denotes	not
T7948	34083-34090	VBN	denotes	altered
T7954	34091-34095	IN	denotes	with
T7955	34096-34101	NN	denotes	aging
T7956	34102-34103	-LRB-	denotes	(
T7957	34103-34105	NNP	denotes	N.
T7958	34106-34112	NNP	denotes	Torabi
T7959	34113-34116	CC	denotes	and
T7960	34117-34119	NNP	denotes	S.
T7961	34120-34127	NNP	denotes	Richard
T7962	34127-34129	,	denotes	,
T7963	34129-34140	JJ	denotes	unpublished
T7964	34141-34145	NNS	denotes	data
T7965	34145-34146	-RRB-	denotes	)
T7966	34146-34148	,	denotes	,
T7967	34148-34158	VBG	denotes	suggesting
T7968	34159-34163	IN	denotes	that
T7970	34164-34167	DT	denotes	the
T7972	34168-34176	VBN	denotes	observed
T7973	34177-34183	NN	denotes	leptin
T7971	34184-34193	NN	denotes	reduction
T7974	34194-34197	VBD	denotes	was
T7975	34198-34201	RB	denotes	not
T7969	34202-34212	VBG	denotes	recreating
T7976	34213-34215	NN	denotes	ob
T7978	34215-34216	HYPH	denotes	/
T7979	34216-34218	NN	denotes	ob
T7980	34218-34219	HYPH	denotes	-
T7977	34219-34223	JJ	denotes	like
T7981	34224-34234	NNS	denotes	phenotypes
T7982	34235-34242	VBN	denotes	related
T7983	34243-34245	IN	denotes	to
T7984	34246-34252	NN	denotes	weight
T7985	34252-34254	,	denotes	,
T7986	34254-34262	NN	denotes	appetite
T7987	34263-34266	CC	denotes	and
T7988	34267-34273	JJ	denotes	female
T7989	34274-34283	NN	denotes	sterility
T7990	34284-34285	-LRB-	denotes	[
T7991	34285-34287	CD	denotes	54
T7992	34287-34288	-RRB-	denotes	]
T7993	34288-34289	.	denotes	.
T7994	34289-34414	sentence	denotes	The skeletal phenotyping of cohorts of Sam68+/+ and Sam68−/− mice showed that bone mass was preserved in aged Sam68−/− mice.
T7995	34290-34293	DT	denotes	The
T7997	34294-34302	JJ	denotes	skeletal
T7996	34303-34314	NN	denotes	phenotyping
T7999	34315-34317	IN	denotes	of
T8000	34318-34325	NNS	denotes	cohorts
T8001	34326-34328	IN	denotes	of
T8002	34329-34334	NN	denotes	Sam68
T8004	34334-34335	SYM	denotes	+
T8005	34335-34336	HYPH	denotes	/
T8006	34336-34337	SYM	denotes	+
T8007	34338-34341	CC	denotes	and
T8008	34342-34347	NN	denotes	Sam68
T8009	34347-34348	SYM	denotes	−
T8010	34348-34349	HYPH	denotes	/
T8011	34349-34350	SYM	denotes	−
T8003	34351-34355	NNS	denotes	mice
T7998	34356-34362	VBD	denotes	showed
T8012	34363-34367	IN	denotes	that
T8014	34368-34372	NN	denotes	bone
T8015	34373-34377	NN	denotes	mass
T8016	34378-34381	VBD	denotes	was
T8013	34382-34391	VBN	denotes	preserved
T8017	34392-34394	IN	denotes	in
T8018	34395-34399	JJ	denotes	aged
T8020	34400-34405	NN	denotes	Sam68
T8021	34405-34406	SYM	denotes	−
T8022	34406-34407	HYPH	denotes	/
T8023	34407-34408	SYM	denotes	−
T8019	34409-34413	NNS	denotes	mice
T8024	34413-34414	.	denotes	.
T8025	34414-34547	sentence	denotes	Traditional histology and histomorphometry suggested that the mechanism involved preservation of osteoblast and osteoclast activity.
T8026	34415-34426	JJ	denotes	Traditional
T8027	34427-34436	NN	denotes	histology
T8029	34437-34440	CC	denotes	and
T8030	34441-34457	NN	denotes	histomorphometry
T8028	34458-34467	VBD	denotes	suggested
T8031	34468-34472	IN	denotes	that
T8033	34473-34476	DT	denotes	the
T8034	34477-34486	NN	denotes	mechanism
T8032	34487-34495	VBD	denotes	involved
T8035	34496-34508	NN	denotes	preservation
T8036	34509-34511	IN	denotes	of
T8037	34512-34522	NN	denotes	osteoblast
T8039	34523-34526	CC	denotes	and
T8040	34527-34537	NN	denotes	osteoclast
T8038	34538-34546	NN	denotes	activity
T8041	34546-34547	DT	denotes	.
T8042	34547-34705	sentence	denotes	The documented role of the Sam68 regulatory protein, Src, in osteopetrosis led us to investigate the morphology and activity of Sam68−/− osteoclasts ex vivo.
T8043	34548-34551	DT	denotes	The
T8045	34552-34562	VBN	denotes	documented
T8044	34563-34567	NN	denotes	role
T8047	34568-34570	IN	denotes	of
T8048	34571-34574	DT	denotes	the
T8050	34575-34580	NN	denotes	Sam68
T8051	34581-34591	JJ	denotes	regulatory
T8049	34592-34599	NN	denotes	protein
T8052	34599-34601	,	denotes	,
T8053	34601-34604	NN	denotes	Src
T8054	34604-34606	,	denotes	,
T8055	34606-34608	IN	denotes	in
T8056	34609-34622	NN	denotes	osteopetrosis
T8046	34623-34626	VBD	denotes	led
T8057	34627-34629	PRP	denotes	us
T8058	34630-34632	TO	denotes	to
T8059	34633-34644	VB	denotes	investigate
T8060	34645-34648	DT	denotes	the
T8061	34649-34659	NN	denotes	morphology
T8062	34660-34663	CC	denotes	and
T8063	34664-34672	NN	denotes	activity
T8064	34673-34675	IN	denotes	of
T8065	34676-34681	NN	denotes	Sam68
T8067	34681-34682	SYM	denotes	−
T8068	34682-34683	HYPH	denotes	/
T8069	34683-34684	SYM	denotes	−
T8066	34685-34696	NNS	denotes	osteoclasts
T8070	34697-34699	FW	denotes	ex
T8071	34700-34704	FW	denotes	vivo
T8072	34704-34705	.	denotes	.
T8073	34705-34923	sentence	denotes	The Src tyrosine kinase was shown to play a role in bone remodeling when Src−/− mice died at 6 months of age with an osteopetrotic phenotype [48] and the defect was attributed to defective osteoclast function [55−59].
T8074	34706-34709	DT	denotes	The
T8076	34710-34713	NN	denotes	Src
T8077	34714-34722	NN	denotes	tyrosine
T8075	34723-34729	NN	denotes	kinase
T8079	34730-34733	VBD	denotes	was
T8078	34734-34739	VBN	denotes	shown
T8080	34740-34742	TO	denotes	to
T8081	34743-34747	VB	denotes	play
T8082	34748-34749	DT	denotes	a
T8083	34750-34754	NN	denotes	role
T8084	34755-34757	IN	denotes	in
T8085	34758-34762	NN	denotes	bone
T8086	34763-34773	NN	denotes	remodeling
T8087	34774-34778	WRB	denotes	when
T8089	34779-34782	NN	denotes	Src
T8091	34782-34783	SYM	denotes	−
T8092	34783-34784	HYPH	denotes	/
T8093	34784-34785	SYM	denotes	−
T8090	34786-34790	NNS	denotes	mice
T8088	34791-34795	VBD	denotes	died
T8094	34796-34798	IN	denotes	at
T8095	34799-34800	CD	denotes	6
T8096	34801-34807	NNS	denotes	months
T8097	34808-34810	IN	denotes	of
T8098	34811-34814	NN	denotes	age
T8099	34815-34819	IN	denotes	with
T8100	34820-34822	DT	denotes	an
T8102	34823-34836	JJ	denotes	osteopetrotic
T8101	34837-34846	NN	denotes	phenotype
T8103	34847-34848	-LRB-	denotes	[
T8104	34848-34850	CD	denotes	48
T8105	34850-34851	-RRB-	denotes	]
T8106	34852-34855	CC	denotes	and
T8107	34856-34859	DT	denotes	the
T8108	34860-34866	NN	denotes	defect
T8110	34867-34870	VBD	denotes	was
T8109	34871-34881	VBN	denotes	attributed
T8111	34882-34884	IN	denotes	to
T8112	34885-34894	JJ	denotes	defective
T8114	34895-34905	NN	denotes	osteoclast
T8113	34906-34914	NN	denotes	function
T8115	34915-34916	-LRB-	denotes	[
T8116	34916-34918	CD	denotes	55
T8117	34918-34919	SYM	denotes	−
T8118	34919-34921	CD	denotes	59
T8119	34921-34922	-RRB-	denotes	]
T8120	34922-34923	.	denotes	.
T8121	34923-35070	sentence	denotes	We therefore cultured mature osteoclasts harvested from Sam68+/+ and Sam68−/− mice ex vivo on dentin slices to quantify their resorptive capacity.
T8122	34924-34926	PRP	denotes	We
T8124	34927-34936	RB	denotes	therefore
T8123	34937-34945	VBD	denotes	cultured
T8125	34946-34952	JJ	denotes	mature
T8126	34953-34964	NNS	denotes	osteoclasts
T8127	34965-34974	VBN	denotes	harvested
T8128	34975-34979	IN	denotes	from
T8129	34980-34985	NN	denotes	Sam68
T8131	34985-34986	SYM	denotes	+
T8132	34986-34987	HYPH	denotes	/
T8133	34987-34988	SYM	denotes	+
T8134	34989-34992	CC	denotes	and
T8135	34993-34998	NN	denotes	Sam68
T8136	34998-34999	SYM	denotes	−
T8137	34999-35000	HYPH	denotes	/
T8138	35000-35001	SYM	denotes	−
T8130	35002-35006	NNS	denotes	mice
T8139	35007-35009	FW	denotes	ex
T8140	35010-35014	FW	denotes	vivo
T8141	35015-35017	IN	denotes	on
T8142	35018-35024	NN	denotes	dentin
T8143	35025-35031	NNS	denotes	slices
T8144	35032-35034	TO	denotes	to
T8145	35035-35043	VB	denotes	quantify
T8146	35044-35049	PRP$	denotes	their
T8148	35050-35060	JJ	denotes	resorptive
T8147	35061-35069	NN	denotes	capacity
T8149	35069-35070	.	denotes	.
T8150	35070-35286	sentence	denotes	The fact that Sam68−/− osteoclasts looked and acted like Sam68+/+ osteoclasts ex vivo and in vivo made it unlikely that this was the primary source of the difference in bone metabolism in 12-month-old Sam68−/− mice.
T8151	35071-35074	DT	denotes	The
T8152	35075-35079	NN	denotes	fact
T8154	35080-35084	IN	denotes	that
T8156	35085-35090	NN	denotes	Sam68
T8158	35090-35091	SYM	denotes	−
T8159	35091-35092	HYPH	denotes	/
T8160	35092-35093	SYM	denotes	−
T8157	35094-35105	NNS	denotes	osteoclasts
T8155	35106-35112	VBD	denotes	looked
T8161	35113-35116	CC	denotes	and
T8162	35117-35122	VBD	denotes	acted
T8163	35123-35127	IN	denotes	like
T8164	35128-35133	NN	denotes	Sam68
T8166	35133-35134	SYM	denotes	+
T8167	35134-35135	HYPH	denotes	/
T8168	35135-35136	SYM	denotes	+
T8165	35137-35148	NNS	denotes	osteoclasts
T8169	35149-35151	FW	denotes	ex
T8170	35152-35156	FW	denotes	vivo
T8171	35157-35160	CC	denotes	and
T8172	35161-35163	FW	denotes	in
T8173	35164-35168	FW	denotes	vivo
T8153	35169-35173	VBD	denotes	made
T8174	35174-35176	PRP	denotes	it
T8175	35177-35185	JJ	denotes	unlikely
T8176	35186-35190	IN	denotes	that
T8178	35191-35195	DT	denotes	this
T8177	35196-35199	VBD	denotes	was
T8179	35200-35203	DT	denotes	the
T8181	35204-35211	JJ	denotes	primary
T8180	35212-35218	NN	denotes	source
T8182	35219-35221	IN	denotes	of
T8183	35222-35225	DT	denotes	the
T8184	35226-35236	NN	denotes	difference
T8185	35237-35239	IN	denotes	in
T8186	35240-35244	NN	denotes	bone
T8187	35245-35255	NN	denotes	metabolism
T8188	35256-35258	IN	denotes	in
T8189	35259-35261	CD	denotes	12
T8191	35261-35262	HYPH	denotes	-
T8190	35262-35267	NN	denotes	month
T8193	35267-35268	HYPH	denotes	-
T8192	35268-35271	JJ	denotes	old
T8195	35272-35277	NN	denotes	Sam68
T8196	35277-35278	SYM	denotes	−
T8197	35278-35279	HYPH	denotes	/
T8198	35279-35280	SYM	denotes	−
T8194	35281-35285	NNS	denotes	mice
T8199	35285-35286	.	denotes	.
T8200	35286-35450	sentence	denotes	The fact that the CTX levels were lower in young and old Sam68−/− mice suggested that there is reduced bone resorption compared with wild-type littermate controls.
T8201	35287-35290	DT	denotes	The
T8202	35291-35295	NN	denotes	fact
T8204	35296-35300	IN	denotes	that
T8206	35301-35304	DT	denotes	the
T8208	35305-35308	NN	denotes	CTX
T8207	35309-35315	NNS	denotes	levels
T8205	35316-35320	VBD	denotes	were
T8209	35321-35326	JJR	denotes	lower
T8210	35327-35329	IN	denotes	in
T8211	35330-35335	JJ	denotes	young
T8213	35336-35339	CC	denotes	and
T8214	35340-35343	JJ	denotes	old
T8215	35344-35349	NN	denotes	Sam68
T8216	35349-35350	SYM	denotes	−
T8217	35350-35351	HYPH	denotes	/
T8218	35351-35352	SYM	denotes	−
T8212	35353-35357	NNS	denotes	mice
T8203	35358-35367	VBD	denotes	suggested
T8219	35368-35372	IN	denotes	that
T8221	35373-35378	EX	denotes	there
T8220	35379-35381	VBZ	denotes	is
T8222	35382-35389	VBN	denotes	reduced
T8224	35390-35394	NN	denotes	bone
T8223	35395-35405	NN	denotes	resorption
T8225	35406-35414	VBN	denotes	compared
T8226	35415-35419	IN	denotes	with
T8227	35420-35424	JJ	denotes	wild
T8229	35424-35425	HYPH	denotes	-
T8228	35425-35429	NN	denotes	type
T8231	35430-35440	NN	denotes	littermate
T8230	35441-35449	NNS	denotes	controls
T8232	35449-35450	.	denotes	.
T8233	35450-35570	sentence	denotes	However, this reduction in bone resorption occurred with normal osteoclast activity, as assessed by in vitro culturing.
T8234	35451-35458	RB	denotes	However
T8236	35458-35460	,	denotes	,
T8237	35460-35464	DT	denotes	this
T8238	35465-35474	NN	denotes	reduction
T8239	35475-35477	IN	denotes	in
T8240	35478-35482	NN	denotes	bone
T8241	35483-35493	NN	denotes	resorption
T8235	35494-35502	VBD	denotes	occurred
T8242	35503-35507	IN	denotes	with
T8243	35508-35514	JJ	denotes	normal
T8245	35515-35525	NN	denotes	osteoclast
T8244	35526-35534	NN	denotes	activity
T8246	35534-35536	,	denotes	,
T8247	35536-35538	IN	denotes	as
T8248	35539-35547	VBN	denotes	assessed
T8249	35548-35550	IN	denotes	by
T8250	35551-35553	FW	denotes	in
T8251	35554-35559	FW	denotes	vitro
T8252	35560-35569	NN	denotes	culturing
T8253	35569-35570	.	denotes	.
T8254	35570-35663	sentence	denotes	These observations are consistent with the Sam68−/− mice having a youth-like bone phenotype.
T8255	35571-35576	DT	denotes	These
T8256	35577-35589	NNS	denotes	observations
T8257	35590-35593	VBP	denotes	are
T8258	35594-35604	JJ	denotes	consistent
T8259	35605-35609	IN	denotes	with
T8260	35610-35613	DT	denotes	the
T8262	35614-35619	NN	denotes	Sam68
T8263	35619-35620	SYM	denotes	−
T8264	35620-35621	HYPH	denotes	/
T8265	35621-35622	SYM	denotes	−
T8261	35623-35627	NNS	denotes	mice
T8266	35628-35634	VBG	denotes	having
T8267	35635-35636	DT	denotes	a
T8269	35637-35642	NN	denotes	youth
T8271	35642-35643	HYPH	denotes	-
T8270	35643-35647	JJ	denotes	like
T8272	35648-35652	NN	denotes	bone
T8268	35653-35662	NN	denotes	phenotype
T8273	35662-35663	.	denotes	.
T8274	35663-35868	sentence	denotes	However, it is still possible, that a mild impairment in Sam68−/− mice osteoclast function may manifest itself later in life in overall accumulation of bone and this will require further detailed studies.
T8275	35664-35671	RB	denotes	However
T8277	35671-35673	,	denotes	,
T8278	35673-35675	PRP	denotes	it
T8276	35676-35678	VBZ	denotes	is
T8279	35679-35684	RB	denotes	still
T8280	35685-35693	JJ	denotes	possible
T8281	35693-35695	,	denotes	,
T8282	35695-35699	IN	denotes	that
T8284	35700-35701	DT	denotes	a
T8286	35702-35706	JJ	denotes	mild
T8285	35707-35717	NN	denotes	impairment
T8287	35718-35720	IN	denotes	in
T8288	35721-35726	NN	denotes	Sam68
T8290	35726-35727	SYM	denotes	−
T8291	35727-35728	HYPH	denotes	/
T8292	35728-35729	SYM	denotes	−
T8289	35730-35734	NNS	denotes	mice
T8294	35735-35745	NN	denotes	osteoclast
T8293	35746-35754	NN	denotes	function
T8295	35755-35758	MD	denotes	may
T8283	35759-35767	VB	denotes	manifest
T8296	35768-35774	PRP	denotes	itself
T8297	35775-35780	RB	denotes	later
T8298	35781-35783	IN	denotes	in
T8299	35784-35788	NN	denotes	life
T8300	35789-35791	IN	denotes	in
T8301	35792-35799	JJ	denotes	overall
T8302	35800-35812	NN	denotes	accumulation
T8303	35813-35815	IN	denotes	of
T8304	35816-35820	NN	denotes	bone
T8305	35821-35824	CC	denotes	and
T8306	35825-35829	DT	denotes	this
T8308	35830-35834	MD	denotes	will
T8307	35835-35842	VB	denotes	require
T8309	35843-35850	JJ	denotes	further
T8311	35851-35859	JJ	denotes	detailed
T8310	35860-35867	NNS	denotes	studies
T8312	35867-35868	.	denotes	.
T8313	35868-36091	sentence	denotes	Ex vivo differentiation of primary bone marrow stromal cells, harvested from Sam68+/+ and Sam68−/− mice, down the osteoblast lineage revealed an osteogenic advantage in the cultures of cells derived from the Sam68−/− mice.
T8314	35869-35871	FW	denotes	Ex
T8315	35872-35876	FW	denotes	vivo
T8316	35877-35892	NN	denotes	differentiation
T8318	35893-35895	IN	denotes	of
T8319	35896-35903	JJ	denotes	primary
T8321	35904-35908	NN	denotes	bone
T8322	35909-35915	NN	denotes	marrow
T8323	35916-35923	JJ	denotes	stromal
T8320	35924-35929	NNS	denotes	cells
T8324	35929-35931	,	denotes	,
T8325	35931-35940	VBN	denotes	harvested
T8326	35941-35945	IN	denotes	from
T8327	35946-35951	NN	denotes	Sam68
T8329	35951-35952	SYM	denotes	+
T8330	35952-35953	HYPH	denotes	/
T8331	35953-35954	SYM	denotes	+
T8332	35955-35958	CC	denotes	and
T8333	35959-35964	NN	denotes	Sam68
T8334	35964-35965	SYM	denotes	−
T8335	35965-35966	HYPH	denotes	/
T8336	35966-35967	SYM	denotes	−
T8328	35968-35972	NNS	denotes	mice
T8337	35972-35974	,	denotes	,
T8338	35974-35978	IN	denotes	down
T8339	35979-35982	DT	denotes	the
T8341	35983-35993	NN	denotes	osteoblast
T8340	35994-36001	NN	denotes	lineage
T8317	36002-36010	VBD	denotes	revealed
T8342	36011-36013	DT	denotes	an
T8344	36014-36024	JJ	denotes	osteogenic
T8343	36025-36034	NN	denotes	advantage
T8345	36035-36037	IN	denotes	in
T8346	36038-36041	DT	denotes	the
T8347	36042-36050	NNS	denotes	cultures
T8348	36051-36053	IN	denotes	of
T8349	36054-36059	NNS	denotes	cells
T8350	36060-36067	VBN	denotes	derived
T8351	36068-36072	IN	denotes	from
T8352	36073-36076	DT	denotes	the
T8354	36077-36082	NN	denotes	Sam68
T8355	36082-36083	SYM	denotes	−
T8356	36083-36084	HYPH	denotes	/
T8357	36084-36085	SYM	denotes	−
T8353	36086-36090	NNS	denotes	mice
T8358	36090-36091	.	denotes	.
T8359	36091-36226	sentence	denotes	It will be important to demonstrate that a similar effect is observed using primary bone marrow stromal cells from aged Sam68−/− mice.
T8360	36092-36094	PRP	denotes	It
T8362	36095-36099	MD	denotes	will
T8361	36100-36102	VB	denotes	be
T8363	36103-36112	JJ	denotes	important
T8364	36113-36115	TO	denotes	to
T8365	36116-36127	VB	denotes	demonstrate
T8366	36128-36132	IN	denotes	that
T8368	36133-36134	DT	denotes	a
T8370	36135-36142	JJ	denotes	similar
T8369	36143-36149	NN	denotes	effect
T8371	36150-36152	VBZ	denotes	is
T8367	36153-36161	VBN	denotes	observed
T8372	36162-36167	VBG	denotes	using
T8373	36168-36175	JJ	denotes	primary
T8375	36176-36180	NN	denotes	bone
T8376	36181-36187	NN	denotes	marrow
T8377	36188-36195	JJ	denotes	stromal
T8374	36196-36201	NNS	denotes	cells
T8378	36202-36206	IN	denotes	from
T8379	36207-36211	JJ	denotes	aged
T8381	36212-36217	NN	denotes	Sam68
T8382	36217-36218	SYM	denotes	−
T8383	36218-36219	HYPH	denotes	/
T8384	36219-36220	SYM	denotes	−
T8380	36221-36225	NNS	denotes	mice
T8385	36225-36226	.	denotes	.
T8386	36226-36400	sentence	denotes	Similar findings were observed when embryonic mesenchymal multipotential progenitor cells C3H10T1/2 treated with Sam68 shRNA, were differentiated into osteoblasts with BMP2.
T8387	36227-36234	JJ	denotes	Similar
T8388	36235-36243	NNS	denotes	findings
T8390	36244-36248	VBD	denotes	were
T8389	36249-36257	VBN	denotes	observed
T8391	36258-36262	WRB	denotes	when
T8393	36263-36272	JJ	denotes	embryonic
T8395	36273-36284	JJ	denotes	mesenchymal
T8396	36285-36299	JJ	denotes	multipotential
T8397	36300-36310	NN	denotes	progenitor
T8394	36311-36316	NNS	denotes	cells
T8398	36317-36324	NN	denotes	C3H10T1
T8399	36324-36325	HYPH	denotes	/
T8400	36325-36326	CD	denotes	2
T8401	36327-36334	VBN	denotes	treated
T8402	36335-36339	IN	denotes	with
T8403	36340-36345	NN	denotes	Sam68
T8404	36346-36351	NN	denotes	shRNA
T8405	36351-36353	,	denotes	,
T8406	36353-36357	VBD	denotes	were
T8392	36358-36372	VBN	denotes	differentiated
T8407	36373-36377	IN	denotes	into
T8408	36378-36389	NNS	denotes	osteoblasts
T8409	36390-36394	IN	denotes	with
T8410	36395-36399	NN	denotes	BMP2
T8411	36399-36400	.	denotes	.
T8412	36400-36630	sentence	denotes	Our findings that aged Sam68−/− mice do not develop fatty bone marrow and that MEFs derived from Sam68 −/− mice have impaired adipocyte differentiation, indicate that Sam68 regulates both adipocyte and osteoblast differentiation.
T8413	36401-36404	PRP$	denotes	Our
T8414	36405-36413	NNS	denotes	findings
T8416	36414-36418	IN	denotes	that
T8418	36419-36423	JJ	denotes	aged
T8420	36424-36429	NN	denotes	Sam68
T8421	36429-36430	SYM	denotes	−
T8422	36430-36431	HYPH	denotes	/
T8423	36431-36432	SYM	denotes	−
T8419	36433-36437	NNS	denotes	mice
T8424	36438-36440	VBP	denotes	do
T8425	36441-36444	RB	denotes	not
T8417	36445-36452	VB	denotes	develop
T8426	36453-36458	JJ	denotes	fatty
T8428	36459-36463	NN	denotes	bone
T8427	36464-36470	NN	denotes	marrow
T8429	36471-36474	CC	denotes	and
T8430	36475-36479	IN	denotes	that
T8432	36480-36484	NNS	denotes	MEFs
T8433	36485-36492	VBN	denotes	derived
T8434	36493-36497	IN	denotes	from
T8435	36498-36503	NN	denotes	Sam68
T8437	36504-36505	SYM	denotes	−
T8438	36505-36506	HYPH	denotes	/
T8439	36506-36507	SYM	denotes	−
T8436	36508-36512	NNS	denotes	mice
T8431	36513-36517	VBP	denotes	have
T8440	36518-36526	VBN	denotes	impaired
T8442	36527-36536	NN	denotes	adipocyte
T8441	36537-36552	NN	denotes	differentiation
T8443	36552-36554	,	denotes	,
T8415	36554-36562	VBP	denotes	indicate
T8444	36563-36567	IN	denotes	that
T8446	36568-36573	NN	denotes	Sam68
T8445	36574-36583	VBZ	denotes	regulates
T8447	36584-36588	CC	denotes	both
T8448	36589-36598	NN	denotes	adipocyte
T8450	36599-36602	CC	denotes	and
T8451	36603-36613	NN	denotes	osteoblast
T8449	36614-36629	NN	denotes	differentiation
T8452	36629-36630	.	denotes	.
T8453	36630-36840	sentence	denotes	These findings identify Sam68 as the first RNA binding protein to regulate mesenchymal cell differentiation and the challenge will be to identify the specific RNA targets that it regulates during this process.
T8454	36631-36636	DT	denotes	These
T8455	36637-36645	NNS	denotes	findings
T8456	36646-36654	VBP	denotes	identify
T8457	36655-36660	NN	denotes	Sam68
T8458	36661-36663	IN	denotes	as
T8459	36664-36667	DT	denotes	the
T8461	36668-36673	JJ	denotes	first
T8462	36674-36677	NN	denotes	RNA
T8463	36678-36685	VBG	denotes	binding
T8460	36686-36693	NN	denotes	protein
T8464	36694-36696	TO	denotes	to
T8465	36697-36705	VB	denotes	regulate
T8466	36706-36717	JJ	denotes	mesenchymal
T8468	36718-36722	NN	denotes	cell
T8467	36723-36738	NN	denotes	differentiation
T8469	36739-36742	CC	denotes	and
T8470	36743-36746	DT	denotes	the
T8471	36747-36756	NN	denotes	challenge
T8473	36757-36761	MD	denotes	will
T8472	36762-36764	VB	denotes	be
T8474	36765-36767	TO	denotes	to
T8475	36768-36776	VB	denotes	identify
T8476	36777-36780	DT	denotes	the
T8478	36781-36789	JJ	denotes	specific
T8479	36790-36793	NN	denotes	RNA
T8477	36794-36801	NNS	denotes	targets
T8480	36802-36806	WDT	denotes	that
T8482	36807-36809	PRP	denotes	it
T8481	36810-36819	VBZ	denotes	regulates
T8483	36820-36826	IN	denotes	during
T8484	36827-36831	DT	denotes	this
T8485	36832-36839	NN	denotes	process
T8486	36839-36840	.	denotes	.
T8487	36840-37026	sentence	denotes	The fact that osteoblast function is altered in Src−/− mice [60,61] raises the possibility that preservation of bone mass in the Sam68−/− mice could be linked with and regulated by Src.
T8488	36841-36844	DT	denotes	The
T8489	36845-36849	NN	denotes	fact
T8491	36850-36854	IN	denotes	that
T8493	36855-36865	NN	denotes	osteoblast
T8494	36866-36874	NN	denotes	function
T8495	36875-36877	VBZ	denotes	is
T8492	36878-36885	VBN	denotes	altered
T8496	36886-36888	IN	denotes	in
T8497	36889-36892	NN	denotes	Src
T8499	36892-36893	SYM	denotes	−
T8500	36893-36894	HYPH	denotes	/
T8501	36894-36895	SYM	denotes	−
T8498	36896-36900	NNS	denotes	mice
T8502	36901-36902	-LRB-	denotes	[
T8504	36902-36904	CD	denotes	60
T8505	36904-36905	,	denotes	,
T8503	36905-36907	CD	denotes	61
T8506	36907-36908	-RRB-	denotes	]
T8490	36909-36915	VBZ	denotes	raises
T8507	36916-36919	DT	denotes	the
T8508	36920-36931	NN	denotes	possibility
T8509	36932-36936	IN	denotes	that
T8511	36937-36949	NN	denotes	preservation
T8512	36950-36952	IN	denotes	of
T8513	36953-36957	NN	denotes	bone
T8514	36958-36962	NN	denotes	mass
T8515	36963-36965	IN	denotes	in
T8516	36966-36969	DT	denotes	the
T8518	36970-36975	NN	denotes	Sam68
T8519	36975-36976	SYM	denotes	−
T8520	36976-36977	HYPH	denotes	/
T8521	36977-36978	SYM	denotes	−
T8517	36979-36983	NNS	denotes	mice
T8522	36984-36989	MD	denotes	could
T8523	36990-36992	VB	denotes	be
T8510	36993-36999	VBN	denotes	linked
T8524	37000-37004	IN	denotes	with
T8525	37005-37008	CC	denotes	and
T8526	37009-37018	VBN	denotes	regulated
T8527	37019-37021	IN	denotes	by
T8528	37022-37025	NN	denotes	Src
T8529	37025-37026	.	denotes	.
T8530	37026-37300	sentence	denotes	The pathway by which leptin regulates bone resorption was identified to involve the sympathetic nervous system relaying to the osteoblasts via the β-adrenergic pathway leading to the release of growth factors including RANKL that causes the osteoclasts to thrive [8,18–20].
T8531	37027-37030	DT	denotes	The
T8532	37031-37038	NN	denotes	pathway
T8534	37039-37041	IN	denotes	by
T8536	37042-37047	WDT	denotes	which
T8537	37048-37054	NN	denotes	leptin
T8535	37055-37064	VBZ	denotes	regulates
T8538	37065-37069	NN	denotes	bone
T8539	37070-37080	NN	denotes	resorption
T8540	37081-37084	VBD	denotes	was
T8533	37085-37095	VBN	denotes	identified
T8541	37096-37098	TO	denotes	to
T8542	37099-37106	VB	denotes	involve
T8543	37107-37110	DT	denotes	the
T8545	37111-37122	JJ	denotes	sympathetic
T8546	37123-37130	JJ	denotes	nervous
T8544	37131-37137	NN	denotes	system
T8547	37138-37146	VBG	denotes	relaying
T8548	37147-37149	IN	denotes	to
T8549	37150-37153	DT	denotes	the
T8550	37154-37165	NNS	denotes	osteoblasts
T8551	37166-37169	IN	denotes	via
T8552	37170-37173	DT	denotes	the
T8554	37174-37175	NN	denotes	β
T8556	37175-37176	HYPH	denotes	-
T8555	37176-37186	JJ	denotes	adrenergic
T8553	37187-37194	NN	denotes	pathway
T8557	37195-37202	VBG	denotes	leading
T8558	37203-37205	IN	denotes	to
T8559	37206-37209	DT	denotes	the
T8560	37210-37217	NN	denotes	release
T8561	37218-37220	IN	denotes	of
T8562	37221-37227	NN	denotes	growth
T8563	37228-37235	NNS	denotes	factors
T8564	37236-37245	VBG	denotes	including
T8565	37246-37251	NN	denotes	RANKL
T8566	37252-37256	WDT	denotes	that
T8567	37257-37263	VBZ	denotes	causes
T8568	37264-37267	DT	denotes	the
T8569	37268-37279	NNS	denotes	osteoclasts
T8571	37280-37282	TO	denotes	to
T8570	37283-37289	VB	denotes	thrive
T8572	37290-37291	-LRB-	denotes	[
T8573	37291-37292	CD	denotes	8
T8574	37292-37293	,	denotes	,
T8575	37293-37295	CD	denotes	18
T8576	37295-37296	SYM	denotes	–
T8577	37296-37298	CD	denotes	20
T8578	37298-37299	-RRB-	denotes	]
T8579	37299-37300	.	denotes	.
T8580	37300-37444	sentence	denotes	The lowering of leptin levels in aged Sam68−/− mice is consistent with these mice having a high bone mass compared with their aged littermates.
T8581	37301-37304	DT	denotes	The
T8582	37305-37313	NN	denotes	lowering
T8584	37314-37316	IN	denotes	of
T8585	37317-37323	NN	denotes	leptin
T8586	37324-37330	NNS	denotes	levels
T8587	37331-37333	IN	denotes	in
T8588	37334-37338	JJ	denotes	aged
T8590	37339-37344	NN	denotes	Sam68
T8591	37344-37345	SYM	denotes	−
T8592	37345-37346	HYPH	denotes	/
T8593	37346-37347	SYM	denotes	−
T8589	37348-37352	NNS	denotes	mice
T8583	37353-37355	VBZ	denotes	is
T8594	37356-37366	JJ	denotes	consistent
T8595	37367-37371	IN	denotes	with
T8596	37372-37377	DT	denotes	these
T8597	37378-37382	NNS	denotes	mice
T8598	37383-37389	VBG	denotes	having
T8599	37390-37391	DT	denotes	a
T8601	37392-37396	JJ	denotes	high
T8602	37397-37401	NN	denotes	bone
T8600	37402-37406	NN	denotes	mass
T8603	37407-37415	VBN	denotes	compared
T8604	37416-37420	IN	denotes	with
T8605	37421-37426	PRP$	denotes	their
T8607	37427-37431	JJ	denotes	aged
T8606	37432-37443	NNS	denotes	littermates
T8608	37443-37444	.	denotes	.
T8609	37444-37634	sentence	denotes	These data would suggest that the leptin-sympathetic pathway is unaltered in Sam68−/− mice and that the lowering of leptin may explain the lower levels of CTX in the serum of Sam68−/− mice.
T8610	37445-37450	DT	denotes	These
T8611	37451-37455	NNS	denotes	data
T8613	37456-37461	MD	denotes	would
T8612	37462-37469	VB	denotes	suggest
T8614	37470-37474	IN	denotes	that
T8616	37475-37478	DT	denotes	the
T8618	37479-37485	NN	denotes	leptin
T8620	37485-37486	HYPH	denotes	-
T8619	37486-37497	JJ	denotes	sympathetic
T8617	37498-37505	NN	denotes	pathway
T8615	37506-37508	VBZ	denotes	is
T8621	37509-37518	JJ	denotes	unaltered
T8622	37519-37521	IN	denotes	in
T8623	37522-37527	NN	denotes	Sam68
T8625	37527-37528	SYM	denotes	−
T8626	37528-37529	HYPH	denotes	/
T8627	37529-37530	SYM	denotes	−
T8624	37531-37535	NNS	denotes	mice
T8628	37536-37539	CC	denotes	and
T8629	37540-37544	IN	denotes	that
T8631	37545-37548	DT	denotes	the
T8632	37549-37557	NN	denotes	lowering
T8633	37558-37560	IN	denotes	of
T8634	37561-37567	NN	denotes	leptin
T8635	37568-37571	MD	denotes	may
T8630	37572-37579	VB	denotes	explain
T8636	37580-37583	DT	denotes	the
T8638	37584-37589	JJR	denotes	lower
T8637	37590-37596	NNS	denotes	levels
T8639	37597-37599	IN	denotes	of
T8640	37600-37603	NN	denotes	CTX
T8641	37604-37606	IN	denotes	in
T8642	37607-37610	DT	denotes	the
T8643	37611-37616	NN	denotes	serum
T8644	37617-37619	IN	denotes	of
T8645	37620-37625	NN	denotes	Sam68
T8647	37625-37626	SYM	denotes	−
T8648	37626-37627	HYPH	denotes	/
T8649	37627-37628	SYM	denotes	−
T8646	37629-37633	NNS	denotes	mice
T8650	37633-37634	.	denotes	.
T8651	37634-37938	sentence	denotes	These findings suggest that Sam68 may be regulating bone metabolism at two different levels: (1) the absence of Sam68 results in lower leptin levels that may reduce bone resorption via the sympathetic nervous system and (2) the absence of Sam68 favors osteoblast, rather than adipocyte, differentiation.
T8652	37635-37640	DT	denotes	These
T8653	37641-37649	NNS	denotes	findings
T8654	37650-37657	VBP	denotes	suggest
T8656	37658-37662	IN	denotes	that
T8658	37663-37668	NN	denotes	Sam68
T8659	37669-37672	MD	denotes	may
T8660	37673-37675	VB	denotes	be
T8657	37676-37686	VBG	denotes	regulating
T8661	37687-37691	NN	denotes	bone
T8662	37692-37702	NN	denotes	metabolism
T8663	37703-37705	IN	denotes	at
T8664	37706-37709	CD	denotes	two
T8666	37710-37719	JJ	denotes	different
T8665	37720-37726	NNS	denotes	levels
T8667	37726-37728	:	denotes	:
T8668	37728-37729	-LRB-	denotes	(
T8669	37729-37730	LS	denotes	1
T8670	37730-37731	-RRB-	denotes	)
T8671	37732-37735	DT	denotes	the
T8672	37736-37743	NN	denotes	absence
T8673	37744-37746	IN	denotes	of
T8674	37747-37752	NN	denotes	Sam68
T8655	37753-37760	VBZ	denotes	results
T8675	37761-37763	IN	denotes	in
T8676	37764-37769	JJR	denotes	lower
T8678	37770-37776	NN	denotes	leptin
T8677	37777-37783	NNS	denotes	levels
T8679	37784-37788	WDT	denotes	that
T8681	37789-37792	MD	denotes	may
T8680	37793-37799	VB	denotes	reduce
T8682	37800-37804	NN	denotes	bone
T8683	37805-37815	NN	denotes	resorption
T8684	37816-37819	IN	denotes	via
T8685	37820-37823	DT	denotes	the
T8687	37824-37835	JJ	denotes	sympathetic
T8688	37836-37843	JJ	denotes	nervous
T8686	37844-37850	NN	denotes	system
T8689	37851-37854	CC	denotes	and
T8690	37855-37856	-LRB-	denotes	(
T8691	37856-37857	LS	denotes	2
T8693	37857-37858	-RRB-	denotes	)
T8694	37859-37862	DT	denotes	the
T8695	37863-37870	NN	denotes	absence
T8696	37871-37873	IN	denotes	of
T8697	37874-37879	NN	denotes	Sam68
T8692	37880-37886	VBZ	denotes	favors
T8698	37887-37897	NN	denotes	osteoblast
T8700	37897-37899	,	denotes	,
T8701	37899-37905	RB	denotes	rather
T8702	37906-37910	IN	denotes	than
T8703	37911-37920	NN	denotes	adipocyte
T8704	37920-37922	,	denotes	,
T8699	37922-37937	NN	denotes	differentiation
T8705	37937-37938	.	denotes	.
T8706	37938-38072	sentence	denotes	In conclusion, our data define a physiologic role for Sam68 in bone metabolism and bone marrow mesenchymal stem cell differentiation.
T8707	37939-37941	IN	denotes	In
T8709	37942-37952	NN	denotes	conclusion
T8710	37952-37954	,	denotes	,
T8711	37954-37957	PRP$	denotes	our
T8712	37958-37962	NNS	denotes	data
T8708	37963-37969	VBP	denotes	define
T8713	37970-37971	DT	denotes	a
T8715	37972-37983	JJ	denotes	physiologic
T8714	37984-37988	NN	denotes	role
T8716	37989-37992	IN	denotes	for
T8717	37993-37998	NN	denotes	Sam68
T8718	37999-38001	IN	denotes	in
T8719	38002-38006	NN	denotes	bone
T8720	38007-38017	NN	denotes	metabolism
T8721	38018-38021	CC	denotes	and
T8722	38022-38026	NN	denotes	bone
T8723	38027-38033	NN	denotes	marrow
T8725	38034-38045	JJ	denotes	mesenchymal
T8727	38046-38050	NN	denotes	stem
T8726	38051-38055	NN	denotes	cell
T8724	38056-38071	NN	denotes	differentiation
T8728	38071-38072	.	denotes	.
T8729	38072-38185	sentence	denotes	The bone phenotype observed in Sam68−/− mice imply that inhibitors of Sam68 could prevent age-related bone loss.
T8730	38073-38076	DT	denotes	The
T8732	38077-38081	NN	denotes	bone
T8731	38082-38091	NN	denotes	phenotype
T8734	38092-38100	VBN	denotes	observed
T8735	38101-38103	IN	denotes	in
T8736	38104-38109	NN	denotes	Sam68
T8738	38109-38110	SYM	denotes	−
T8739	38110-38111	HYPH	denotes	/
T8740	38111-38112	SYM	denotes	−
T8737	38113-38117	NNS	denotes	mice
T8733	38118-38123	VBP	denotes	imply
T8741	38124-38128	IN	denotes	that
T8743	38129-38139	NNS	denotes	inhibitors
T8744	38140-38142	IN	denotes	of
T8745	38143-38148	NN	denotes	Sam68
T8746	38149-38154	MD	denotes	could
T8742	38155-38162	VB	denotes	prevent
T8747	38163-38166	NN	denotes	age
T8749	38166-38167	HYPH	denotes	-
T8748	38167-38174	VBN	denotes	related
T8751	38175-38179	NN	denotes	bone
T8750	38180-38184	NN	denotes	loss
T8752	38184-38185	.	denotes	.
T8753	38185-38371	sentence	denotes	Furthermore, the results also suggest that Sam68 expression levels, hypomorphism, and mutations in humans may influence susceptibility to marrow adipocyte accumulation and osteoporosis.
T8754	38186-38197	RB	denotes	Furthermore
T8756	38197-38199	,	denotes	,
T8757	38199-38202	DT	denotes	the
T8758	38203-38210	NNS	denotes	results
T8759	38211-38215	RB	denotes	also
T8755	38216-38223	VBP	denotes	suggest
T8760	38224-38228	IN	denotes	that
T8762	38229-38234	NN	denotes	Sam68
T8764	38235-38245	NN	denotes	expression
T8763	38246-38252	NNS	denotes	levels
T8765	38252-38254	,	denotes	,
T8766	38254-38266	NN	denotes	hypomorphism
T8767	38266-38268	,	denotes	,
T8768	38268-38271	CC	denotes	and
T8769	38272-38281	NNS	denotes	mutations
T8770	38282-38284	IN	denotes	in
T8771	38285-38291	NNS	denotes	humans
T8772	38292-38295	MD	denotes	may
T8761	38296-38305	VB	denotes	influence
T8773	38306-38320	NN	denotes	susceptibility
T8774	38321-38323	IN	denotes	to
T8775	38324-38330	NN	denotes	marrow
T8776	38331-38340	NN	denotes	adipocyte
T8777	38341-38353	NN	denotes	accumulation
T8778	38354-38357	CC	denotes	and
T8779	38358-38370	NN	denotes	osteoporosis
T8780	38370-38371	.	denotes	.
T8781	38371-38443	sentence	denotes	Our findings also identify a new animal model to study aging bone loss.
T8782	38372-38375	PRP$	denotes	Our
T8783	38376-38384	NNS	denotes	findings
T8785	38385-38389	RB	denotes	also
T8784	38390-38398	VBP	denotes	identify
T8786	38399-38400	DT	denotes	a
T8788	38401-38404	JJ	denotes	new
T8789	38405-38411	NN	denotes	animal
T8787	38412-38417	NN	denotes	model
T8790	38418-38420	TO	denotes	to
T8791	38421-38426	VB	denotes	study
T8792	38427-38432	VBG	denotes	aging
T8794	38433-38437	NN	denotes	bone
T8793	38438-38442	NN	denotes	loss
T8795	38442-38443	.	denotes	.
T8863	38468-38478	JJ	denotes	Histologic
T8865	38478-38480	,	denotes	,
T8866	38480-38499	JJ	denotes	immunohistochemical
T8867	38499-38501	,	denotes	,
T8868	38501-38504	CC	denotes	and
T8869	38505-38522	JJ	denotes	histomorphometric
T8864	38523-38531	NNS	denotes	analyses
T8870	38531-38532	.	denotes	.
T8871	38532-38605	sentence	denotes	All analyses were performed essentially as described previously [62,63].
T8872	38533-38536	DT	denotes	All
T8873	38537-38545	NNS	denotes	analyses
T8875	38546-38550	VBD	denotes	were
T8874	38551-38560	VBN	denotes	performed
T8876	38561-38572	RB	denotes	essentially
T8878	38573-38575	IN	denotes	as
T8877	38576-38585	VBN	denotes	described
T8879	38586-38596	RB	denotes	previously
T8880	38597-38598	-LRB-	denotes	[
T8882	38598-38600	CD	denotes	62
T8883	38600-38601	,	denotes	,
T8881	38601-38603	CD	denotes	63
T8884	38603-38604	-RRB-	denotes	]
T8885	38604-38605	.	denotes	.
T8886	38605-38800	sentence	denotes	Briefly, embryonic mice were removed from timed pregnant dams at E14.5 and E16.5 and fixed intact for 36 h in 4% paraformaldehyde, rinsed thoroughly in PBS, and processed for paraffin embedding.
T8887	38606-38613	RB	denotes	Briefly
T8889	38613-38615	,	denotes	,
T8890	38615-38624	JJ	denotes	embryonic
T8891	38625-38629	NNS	denotes	mice
T8892	38630-38634	VBD	denotes	were
T8888	38635-38642	VBN	denotes	removed
T8893	38643-38647	IN	denotes	from
T8894	38648-38653	VBN	denotes	timed
T8896	38654-38662	JJ	denotes	pregnant
T8895	38663-38667	NNS	denotes	dams
T8897	38668-38670	IN	denotes	at
T8898	38671-38676	NN	denotes	E14.5
T8899	38677-38680	CC	denotes	and
T8900	38681-38686	NN	denotes	E16.5
T8901	38687-38690	CC	denotes	and
T8902	38691-38696	VBN	denotes	fixed
T8903	38697-38703	JJ	denotes	intact
T8904	38704-38707	IN	denotes	for
T8905	38708-38710	CD	denotes	36
T8906	38711-38712	NN	denotes	h
T8907	38713-38715	IN	denotes	in
T8908	38716-38717	CD	denotes	4
T8909	38717-38718	NN	denotes	%
T8910	38719-38735	NN	denotes	paraformaldehyde
T8911	38735-38737	,	denotes	,
T8912	38737-38743	VBN	denotes	rinsed
T8913	38744-38754	RB	denotes	thoroughly
T8914	38755-38757	IN	denotes	in
T8915	38758-38761	NN	denotes	PBS
T8916	38761-38763	,	denotes	,
T8917	38763-38766	CC	denotes	and
T8918	38767-38776	VBN	denotes	processed
T8919	38777-38780	IN	denotes	for
T8920	38781-38789	NN	denotes	paraffin
T8921	38790-38799	NN	denotes	embedding
T8922	38799-38800	.	denotes	.
T8923	38800-39055	sentence	denotes	Serial 4-μm sections were cut on a modified Leica RM 2155 rotary microtome (Leica Microsystems, Richmond Hill, Ontario, Canada), stained with a 1:600 dilution of the AD1 anti-Sam68 antibody [46] and counterstained with either methyl green or hematoxylin.
T8924	38801-38807	JJ	denotes	Serial
T8926	38808-38809	CD	denotes	4
T8928	38809-38810	HYPH	denotes	-
T8927	38810-38812	NN	denotes	μm
T8925	38813-38821	NNS	denotes	sections
T8930	38822-38826	VBD	denotes	were
T8929	38827-38830	VBN	denotes	cut
T8931	38831-38833	IN	denotes	on
T8932	38834-38835	DT	denotes	a
T8934	38836-38844	VBN	denotes	modified
T8935	38845-38850	NNP	denotes	Leica
T8936	38851-38853	NNP	denotes	RM
T8937	38854-38858	CD	denotes	2155
T8938	38859-38865	JJ	denotes	rotary
T8933	38866-38875	NN	denotes	microtome
T8939	38876-38877	-LRB-	denotes	(
T8941	38877-38882	NNP	denotes	Leica
T8940	38883-38895	NNP	denotes	Microsystems
T8942	38895-38897	,	denotes	,
T8943	38897-38905	NNP	denotes	Richmond
T8944	38906-38910	NNP	denotes	Hill
T8945	38910-38912	,	denotes	,
T8946	38912-38919	NNP	denotes	Ontario
T8947	38919-38921	,	denotes	,
T8948	38921-38927	NNP	denotes	Canada
T8949	38927-38928	-RRB-	denotes	)
T8950	38928-38930	,	denotes	,
T8951	38930-38937	VBN	denotes	stained
T8952	38938-38942	IN	denotes	with
T8953	38943-38944	DT	denotes	a
T8955	38945-38946	CD	denotes	1
T8956	38946-38947	SYM	denotes	:
T8957	38947-38950	CD	denotes	600
T8954	38951-38959	NN	denotes	dilution
T8958	38960-38962	IN	denotes	of
T8959	38963-38966	DT	denotes	the
T8961	38967-38970	NN	denotes	AD1
T8962	38971-38981	JJ	denotes	anti-Sam68
T8960	38982-38990	NN	denotes	antibody
T8963	38991-38992	-LRB-	denotes	[
T8964	38992-38994	CD	denotes	46
T8965	38994-38995	-RRB-	denotes	]
T8966	38996-38999	CC	denotes	and
T8967	39000-39014	VBN	denotes	counterstained
T8968	39015-39019	IN	denotes	with
T8969	39020-39026	CC	denotes	either
T8971	39027-39033	NN	denotes	methyl
T8970	39034-39039	NN	denotes	green
T8972	39040-39042	CC	denotes	or
T8973	39043-39054	NN	denotes	hematoxylin
T8974	39054-39055	.	denotes	.
T8975	39055-39232	sentence	denotes	Adult mice were given an intraperitoneal injection of 30 mg/kg calcein at 7 days and 30 mg/kg tetracycline at 2 days prior to sacrifice to label actively mineralizing surfaces.
T8976	39056-39061	JJ	denotes	Adult
T8977	39062-39066	NNS	denotes	mice
T8979	39067-39071	VBD	denotes	were
T8978	39072-39077	VBN	denotes	given
T8980	39078-39080	DT	denotes	an
T8982	39081-39096	JJ	denotes	intraperitoneal
T8981	39097-39106	NN	denotes	injection
T8983	39107-39109	IN	denotes	of
T8984	39110-39112	CD	denotes	30
T8985	39113-39115	NN	denotes	mg
T8987	39115-39116	SYM	denotes	/
T8988	39116-39118	NN	denotes	kg
T8986	39119-39126	NN	denotes	calcein
T8989	39127-39129	IN	denotes	at
T8990	39130-39131	CD	denotes	7
T8991	39132-39136	NNS	denotes	days
T8992	39137-39140	CC	denotes	and
T8993	39141-39143	CD	denotes	30
T8994	39144-39146	NN	denotes	mg
T8996	39146-39147	SYM	denotes	/
T8997	39147-39149	NN	denotes	kg
T8995	39150-39162	NN	denotes	tetracycline
T8998	39163-39165	IN	denotes	at
T8999	39166-39167	CD	denotes	2
T9000	39168-39172	NNS	denotes	days
T9001	39173-39178	JJ	denotes	prior
T9002	39179-39181	IN	denotes	to
T9003	39182-39191	NN	denotes	sacrifice
T9004	39192-39194	TO	denotes	to
T9005	39195-39200	VB	denotes	label
T9006	39201-39209	RB	denotes	actively
T9007	39210-39222	VBG	denotes	mineralizing
T9008	39223-39231	NNS	denotes	surfaces
T9009	39231-39232	.	denotes	.
T9010	39232-39429	sentence	denotes	After overnight fixation in 4% paraformaldehyde and rinsing in PBS, the left femur and tibia were embedded in polymethylmethacrylate (MMA) or a mixture of 50% MMA and 50% glycolmethacrylate (GMA).
T9011	39233-39238	IN	denotes	After
T9013	39239-39248	RB	denotes	overnight
T9014	39249-39257	NN	denotes	fixation
T9015	39258-39260	IN	denotes	in
T9016	39261-39262	CD	denotes	4
T9017	39262-39263	NN	denotes	%
T9018	39264-39280	NN	denotes	paraformaldehyde
T9019	39281-39284	CC	denotes	and
T9020	39285-39292	VBG	denotes	rinsing
T9021	39293-39295	IN	denotes	in
T9022	39296-39299	NN	denotes	PBS
T9023	39299-39301	,	denotes	,
T9024	39301-39304	DT	denotes	the
T9026	39305-39309	JJ	denotes	left
T9025	39310-39315	NN	denotes	femur
T9027	39316-39319	CC	denotes	and
T9028	39320-39325	NN	denotes	tibia
T9029	39326-39330	VBD	denotes	were
T9012	39331-39339	VBN	denotes	embedded
T9030	39340-39342	IN	denotes	in
T9031	39343-39365	NN	denotes	polymethylmethacrylate
T9032	39366-39367	-LRB-	denotes	(
T9033	39367-39370	NN	denotes	MMA
T9034	39370-39371	-RRB-	denotes	)
T9035	39372-39374	CC	denotes	or
T9036	39375-39376	DT	denotes	a
T9037	39377-39384	NN	denotes	mixture
T9038	39385-39387	IN	denotes	of
T9039	39388-39390	CD	denotes	50
T9040	39390-39391	NN	denotes	%
T9041	39392-39395	NN	denotes	MMA
T9042	39396-39399	CC	denotes	and
T9043	39400-39402	CD	denotes	50
T9044	39402-39403	NN	denotes	%
T9045	39404-39422	NN	denotes	glycolmethacrylate
T9046	39423-39424	-LRB-	denotes	(
T9047	39424-39427	NN	denotes	GMA
T9048	39427-39428	-RRB-	denotes	)
T9049	39428-39429	.	denotes	.
T9050	39429-39643	sentence	denotes	Serial 4- to 6-μm sections of MMA-embedded tissues were left unstained or stained with von Kossa and toluidine blue or with toluidine blue alone, while 4-μm MMA-GMA sections were stained for TRAP and ALP activity.
T9051	39430-39436	JJ	denotes	Serial
T9053	39437-39438	CD	denotes	4
T9055	39438-39439	HYPH	denotes	-
T9056	39440-39442	IN	denotes	to
T9054	39443-39444	CD	denotes	6
T9058	39444-39445	HYPH	denotes	-
T9057	39445-39447	NN	denotes	μm
T9052	39448-39456	NNS	denotes	sections
T9060	39457-39459	IN	denotes	of
T9061	39460-39463	NN	denotes	MMA
T9063	39463-39464	HYPH	denotes	-
T9062	39464-39472	VBN	denotes	embedded
T9064	39473-39480	NNS	denotes	tissues
T9065	39481-39485	VBD	denotes	were
T9059	39486-39490	VBN	denotes	left
T9066	39491-39500	JJ	denotes	unstained
T9067	39501-39503	CC	denotes	or
T9068	39504-39511	VBN	denotes	stained
T9069	39512-39516	IN	denotes	with
T9070	39517-39520	NNP	denotes	von
T9071	39521-39526	NNP	denotes	Kossa
T9072	39527-39530	CC	denotes	and
T9073	39531-39540	NN	denotes	toluidine
T9074	39541-39545	NN	denotes	blue
T9075	39546-39548	CC	denotes	or
T9076	39549-39553	IN	denotes	with
T9077	39554-39563	NN	denotes	toluidine
T9078	39564-39568	NN	denotes	blue
T9079	39569-39574	RB	denotes	alone
T9080	39574-39576	,	denotes	,
T9081	39576-39581	IN	denotes	while
T9083	39582-39583	CD	denotes	4
T9085	39583-39584	HYPH	denotes	-
T9084	39584-39586	NN	denotes	μm
T9087	39587-39590	NN	denotes	MMA
T9089	39590-39591	HYPH	denotes	-
T9088	39591-39594	NN	denotes	GMA
T9086	39595-39603	NNS	denotes	sections
T9090	39604-39608	VBD	denotes	were
T9082	39609-39616	VBN	denotes	stained
T9091	39617-39620	IN	denotes	for
T9092	39621-39625	NN	denotes	TRAP
T9094	39626-39629	CC	denotes	and
T9095	39630-39633	NN	denotes	ALP
T9093	39634-39642	NN	denotes	activity
T9096	39642-39643	.	denotes	.
T9097	39643-39962	sentence	denotes	Images were captured using a Leica DMR microscope (Leica Microsystems) equipped with a Retiga 1300 camera (Qimaging, Burnaby, British Columbia, Canada) and the primary histomorphometric data obtained using Bioquant Nova Prime image analysis software (Bioquant Image Analysis Corp, Nashville, Tennessee, United States).
T9098	39644-39650	NNS	denotes	Images
T9100	39651-39655	VBD	denotes	were
T9099	39656-39664	VBN	denotes	captured
T9101	39665-39670	VBG	denotes	using
T9102	39671-39672	DT	denotes	a
T9104	39673-39678	NNP	denotes	Leica
T9105	39679-39682	NN	denotes	DMR
T9103	39683-39693	NN	denotes	microscope
T9106	39694-39695	-LRB-	denotes	(
T9108	39695-39700	NNP	denotes	Leica
T9107	39701-39713	NNP	denotes	Microsystems
T9109	39713-39714	-RRB-	denotes	)
T9110	39715-39723	VBN	denotes	equipped
T9111	39724-39728	IN	denotes	with
T9112	39729-39730	DT	denotes	a
T9114	39731-39737	NNP	denotes	Retiga
T9115	39738-39742	CD	denotes	1300
T9113	39743-39749	NN	denotes	camera
T9116	39750-39751	-LRB-	denotes	(
T9117	39751-39759	NNP	denotes	Qimaging
T9118	39759-39761	,	denotes	,
T9119	39761-39768	NNP	denotes	Burnaby
T9120	39768-39770	,	denotes	,
T9121	39770-39777	NNP	denotes	British
T9122	39778-39786	NNP	denotes	Columbia
T9123	39786-39788	,	denotes	,
T9124	39788-39794	NNP	denotes	Canada
T9125	39794-39795	-RRB-	denotes	)
T9126	39796-39799	CC	denotes	and
T9127	39800-39803	DT	denotes	the
T9129	39804-39811	JJ	denotes	primary
T9130	39812-39829	JJ	denotes	histomorphometric
T9128	39830-39834	NNS	denotes	data
T9131	39835-39843	VBN	denotes	obtained
T9132	39844-39849	VBG	denotes	using
T9133	39850-39858	NNP	denotes	Bioquant
T9135	39859-39863	NNP	denotes	Nova
T9134	39864-39869	NNP	denotes	Prime
T9137	39870-39875	NN	denotes	image
T9138	39876-39884	NN	denotes	analysis
T9136	39885-39893	NN	denotes	software
T9139	39894-39895	-LRB-	denotes	(
T9141	39895-39903	NNP	denotes	Bioquant
T9142	39904-39909	NNP	denotes	Image
T9143	39910-39918	NNP	denotes	Analysis
T9140	39919-39923	NNP	denotes	Corp
T9144	39923-39925	,	denotes	,
T9145	39925-39934	NNP	denotes	Nashville
T9146	39934-39936	,	denotes	,
T9147	39936-39945	NNP	denotes	Tennessee
T9148	39945-39947	,	denotes	,
T9149	39947-39953	NNP	denotes	United
T9150	39954-39960	NNP	denotes	States
T9151	39960-39961	-RRB-	denotes	)
T9152	39961-39962	.	denotes	.
T9153	39962-40082	sentence	denotes	Nomenclature and abbreviations conform to those recommended by the American Society for Bone and Mineral Research [50].
T9154	39963-39975	NN	denotes	Nomenclature
T9156	39976-39979	CC	denotes	and
T9157	39980-39993	NNS	denotes	abbreviations
T9155	39994-40001	VBP	denotes	conform
T9158	40002-40004	IN	denotes	to
T9159	40005-40010	DT	denotes	those
T9160	40011-40022	VBN	denotes	recommended
T9161	40023-40025	IN	denotes	by
T9162	40026-40029	DT	denotes	the
T9164	40030-40038	NNP	denotes	American
T9163	40039-40046	NNP	denotes	Society
T9165	40047-40050	IN	denotes	for
T9166	40051-40055	NNP	denotes	Bone
T9168	40056-40059	CC	denotes	and
T9169	40060-40067	NNP	denotes	Mineral
T9167	40068-40076	NNP	denotes	Research
T9170	40077-40078	-LRB-	denotes	[
T9171	40078-40080	CD	denotes	50
T9172	40080-40081	-RRB-	denotes	]
T9173	40081-40082	.	denotes	.
T9293	40084-40094	NN	denotes	Generation
T9294	40095-40097	IN	denotes	of
T9295	40098-40102	NNS	denotes	mice
T9296	40103-40107	IN	denotes	with
T9297	40108-40116	VBN	denotes	targeted
T9298	40117-40127	NN	denotes	disruption
T9299	40128-40130	IN	denotes	of
T9300	40131-40134	DT	denotes	the
T9302	40135-40140	NN	denotes	sam68
T9301	40141-40145	NN	denotes	gene
T9303	40145-40146	.	denotes	.
T9304	40146-40287	sentence	denotes	A λ bacteriophage clone encompassing Sam68 exons 3 to 9 was isolated from a 129/SvJ genomic library using full-length Sam68 cDNA as a probe.
T9305	40147-40148	DT	denotes	A
T9307	40149-40150	NN	denotes	λ
T9308	40151-40164	NN	denotes	bacteriophage
T9306	40165-40170	NN	denotes	clone
T9310	40171-40183	VBG	denotes	encompassing
T9311	40184-40189	NN	denotes	Sam68
T9313	40190-40195	NNS	denotes	exons
T9312	40196-40197	CD	denotes	3
T9314	40198-40200	IN	denotes	to
T9315	40201-40202	CD	denotes	9
T9316	40203-40206	VBD	denotes	was
T9309	40207-40215	VBN	denotes	isolated
T9317	40216-40220	IN	denotes	from
T9318	40221-40222	DT	denotes	a
T9320	40223-40226	CD	denotes	129
T9322	40226-40227	HYPH	denotes	/
T9321	40227-40230	NN	denotes	SvJ
T9323	40231-40238	JJ	denotes	genomic
T9319	40239-40246	NN	denotes	library
T9324	40247-40252	VBG	denotes	using
T9325	40253-40257	JJ	denotes	full
T9327	40257-40258	HYPH	denotes	-
T9326	40258-40264	NN	denotes	length
T9329	40265-40270	NN	denotes	Sam68
T9328	40271-40275	NN	denotes	cDNA
T9330	40276-40278	IN	denotes	as
T9331	40279-40280	DT	denotes	a
T9332	40281-40286	NN	denotes	probe
T9333	40286-40287	.	denotes	.
T9334	40287-40497	sentence	denotes	XbaI-digested Sam68 genomic DNA fragments of 4 kb (encompassing exon 4 and part of exon 5) and 3kb (spanning part of exon 5 and exon 6) were subcloned in Bluescript SK resulting in pBS4 and pBS3, respectively.
T9335	40288-40292	NN	denotes	XbaI
T9337	40292-40293	HYPH	denotes	-
T9336	40293-40301	VBN	denotes	digested
T9339	40302-40307	NN	denotes	Sam68
T9340	40308-40315	JJ	denotes	genomic
T9341	40316-40319	NN	denotes	DNA
T9338	40320-40329	NNS	denotes	fragments
T9343	40330-40332	IN	denotes	of
T9344	40333-40334	CD	denotes	4
T9345	40335-40337	NN	denotes	kb
T9346	40338-40339	-LRB-	denotes	(
T9347	40339-40351	VBG	denotes	encompassing
T9348	40352-40356	NN	denotes	exon
T9349	40357-40358	CD	denotes	4
T9350	40359-40362	CC	denotes	and
T9351	40363-40367	NN	denotes	part
T9352	40368-40370	IN	denotes	of
T9353	40371-40375	NN	denotes	exon
T9354	40376-40377	CD	denotes	5
T9355	40377-40378	-RRB-	denotes	)
T9356	40379-40382	CC	denotes	and
T9357	40383-40386	NN	denotes	3kb
T9358	40387-40388	-LRB-	denotes	(
T9359	40388-40396	VBG	denotes	spanning
T9360	40397-40401	NN	denotes	part
T9361	40402-40404	IN	denotes	of
T9362	40405-40409	NN	denotes	exon
T9363	40410-40411	CD	denotes	5
T9364	40412-40415	CC	denotes	and
T9365	40416-40420	NN	denotes	exon
T9366	40421-40422	CD	denotes	6
T9367	40422-40423	-RRB-	denotes	)
T9368	40424-40428	VBD	denotes	were
T9342	40429-40438	VBN	denotes	subcloned
T9369	40439-40441	IN	denotes	in
T9370	40442-40452	NN	denotes	Bluescript
T9371	40453-40455	NN	denotes	SK
T9372	40456-40465	VBG	denotes	resulting
T9373	40466-40468	IN	denotes	in
T9374	40469-40473	NN	denotes	pBS4
T9375	40474-40477	CC	denotes	and
T9376	40478-40482	NN	denotes	pBS3
T9377	40482-40484	,	denotes	,
T9378	40484-40496	RB	denotes	respectively
T9379	40496-40497	.	denotes	.
T9380	40497-40644	sentence	denotes	A DNA fragment was amplified from pBS4 with the following oligonucleotides (5′-AAT GTC TAG AAA CAA CTC ATA TAC AGA C-3′) and the universal primer.
T9381	40498-40499	DT	denotes	A
T9383	40500-40503	NN	denotes	DNA
T9382	40504-40512	NN	denotes	fragment
T9385	40513-40516	VBD	denotes	was
T9384	40517-40526	VBN	denotes	amplified
T9386	40527-40531	IN	denotes	from
T9387	40532-40536	NN	denotes	pBS4
T9388	40537-40541	IN	denotes	with
T9389	40542-40545	DT	denotes	the
T9391	40546-40555	JJ	denotes	following
T9390	40556-40572	NNS	denotes	oligonucleotides
T9392	40573-40574	-LRB-	denotes	(
T9393	40574-40575	CD	denotes	5
T9394	40575-40576	SYM	denotes	′
T9395	40576-40577	HYPH	denotes	-
T9396	40577-40580	NN	denotes	AAT
T9398	40581-40584	NN	denotes	GTC
T9399	40585-40588	NN	denotes	TAG
T9400	40589-40592	NN	denotes	AAA
T9401	40593-40596	NN	denotes	CAA
T9402	40597-40600	NN	denotes	CTC
T9403	40601-40604	NN	denotes	ATA
T9404	40605-40608	NN	denotes	TAC
T9405	40609-40612	NN	denotes	AGA
T9397	40613-40614	NN	denotes	C
T9406	40614-40615	HYPH	denotes	-
T9407	40615-40616	CD	denotes	3
T9408	40616-40617	SYM	denotes	′
T9409	40617-40618	-RRB-	denotes	)
T9410	40619-40622	CC	denotes	and
T9411	40623-40626	DT	denotes	the
T9413	40627-40636	JJ	denotes	universal
T9412	40637-40643	NN	denotes	primer
T9414	40643-40644	.	denotes	.
T9415	40644-40795	sentence	denotes	The XbaI-digested 1-kb DNA fragment was subcloned in the XbaI site of pPNT (a gift from Andrew Karaplis, McGill University, Montréal, Quebec, Canada).
T9416	40645-40648	DT	denotes	The
T9418	40649-40653	NN	denotes	XbaI
T9420	40653-40654	HYPH	denotes	-
T9419	40654-40662	VBN	denotes	digested
T9421	40663-40664	CD	denotes	1
T9423	40664-40665	HYPH	denotes	-
T9422	40665-40667	NN	denotes	kb
T9424	40668-40671	NN	denotes	DNA
T9417	40672-40680	NN	denotes	fragment
T9426	40681-40684	VBD	denotes	was
T9425	40685-40694	VBN	denotes	subcloned
T9427	40695-40697	IN	denotes	in
T9428	40698-40701	DT	denotes	the
T9430	40702-40706	NN	denotes	XbaI
T9429	40707-40711	NN	denotes	site
T9431	40712-40714	IN	denotes	of
T9432	40715-40719	NN	denotes	pPNT
T9433	40720-40721	-LRB-	denotes	(
T9435	40721-40722	DT	denotes	a
T9434	40723-40727	NN	denotes	gift
T9436	40728-40732	IN	denotes	from
T9437	40733-40739	NNP	denotes	Andrew
T9438	40740-40748	NNP	denotes	Karaplis
T9439	40748-40750	,	denotes	,
T9440	40750-40756	NNP	denotes	McGill
T9441	40757-40767	NNP	denotes	University
T9442	40767-40769	,	denotes	,
T9443	40769-40777	NNP	denotes	Montréal
T9444	40777-40779	,	denotes	,
T9445	40779-40785	NNP	denotes	Quebec
T9446	40785-40787	,	denotes	,
T9447	40787-40793	NNP	denotes	Canada
T9448	40793-40794	-RRB-	denotes	)
T9449	40794-40795	.	denotes	.
T9450	40795-41009	sentence	denotes	The 3-kb fragment from pBS3 was amplified by PCR with (5′-GGG ATG CGG CCG CTC TAG AAT TGT CCT ACT TGA ACG G-3') and (5′-CGG TGG CGG CCG CTG TCG ACC TGA GTA ACA TTT CTT A-3′) and subcloned in the NotI site of pPNT.
T9451	40796-40799	DT	denotes	The
T9453	40800-40801	CD	denotes	3
T9455	40801-40802	HYPH	denotes	-
T9454	40802-40804	NN	denotes	kb
T9452	40805-40813	NN	denotes	fragment
T9457	40814-40818	IN	denotes	from
T9458	40819-40823	NN	denotes	pBS3
T9459	40824-40827	VBD	denotes	was
T9456	40828-40837	VBN	denotes	amplified
T9460	40838-40840	IN	denotes	by
T9461	40841-40844	NN	denotes	PCR
T9462	40845-40849	IN	denotes	with
T9463	40850-40851	-LRB-	denotes	(
T9464	40851-40852	CD	denotes	5
T9465	40852-40853	SYM	denotes	′
T9466	40853-40854	HYPH	denotes	-
T9467	40854-40857	NN	denotes	GGG
T9469	40858-40861	NN	denotes	ATG
T9470	40862-40865	NN	denotes	CGG
T9471	40866-40869	NN	denotes	CCG
T9472	40870-40873	NN	denotes	CTC
T9473	40874-40877	NN	denotes	TAG
T9474	40878-40881	NN	denotes	AAT
T9475	40882-40885	NN	denotes	TGT
T9476	40886-40889	NN	denotes	CCT
T9477	40890-40893	NN	denotes	ACT
T9478	40894-40897	NN	denotes	TGA
T9479	40898-40901	NN	denotes	ACG
T9468	40902-40903	NN	denotes	G
T9480	40903-40904	HYPH	denotes	-
T9481	40904-40905	CD	denotes	3
T9482	40905-40906	SYM	denotes	'
T9483	40906-40907	-RRB-	denotes	)
T9484	40908-40911	CC	denotes	and
T9485	40912-40913	-LRB-	denotes	(
T9486	40913-40914	CD	denotes	5
T9487	40914-40915	SYM	denotes	′
T9488	40915-40916	HYPH	denotes	-
T9489	40916-40919	NN	denotes	CGG
T9491	40920-40923	NN	denotes	TGG
T9492	40924-40927	NN	denotes	CGG
T9493	40928-40931	NN	denotes	CCG
T9494	40932-40935	NN	denotes	CTG
T9495	40936-40939	NN	denotes	TCG
T9496	40940-40943	NN	denotes	ACC
T9497	40944-40947	NN	denotes	TGA
T9498	40948-40951	NN	denotes	GTA
T9499	40952-40955	NN	denotes	ACA
T9500	40956-40959	NN	denotes	TTT
T9501	40960-40963	NN	denotes	CTT
T9490	40964-40965	NN	denotes	A
T9502	40965-40966	HYPH	denotes	-
T9503	40966-40967	CD	denotes	3
T9504	40967-40968	SYM	denotes	′
T9505	40968-40969	-RRB-	denotes	)
T9506	40970-40973	CC	denotes	and
T9507	40974-40983	VBN	denotes	subcloned
T9508	40984-40986	IN	denotes	in
T9509	40987-40990	DT	denotes	the
T9511	40991-40995	NN	denotes	NotI
T9510	40996-41000	NN	denotes	site
T9512	41001-41003	IN	denotes	of
T9513	41004-41008	NN	denotes	pPNT
T9514	41008-41009	.	denotes	.
T9515	41009-41117	sentence	denotes	The targeting vector pPNT-Sam68 replaces exon 4 and part of exon 5 with a neomycin-resistant gene cassette.
T9516	41010-41013	DT	denotes	The
T9518	41014-41023	NN	denotes	targeting
T9517	41024-41030	NN	denotes	vector
T9520	41031-41035	NN	denotes	pPNT
T9522	41035-41036	HYPH	denotes	-
T9521	41036-41041	NN	denotes	Sam68
T9519	41042-41050	VBZ	denotes	replaces
T9523	41051-41055	NN	denotes	exon
T9524	41056-41057	CD	denotes	4
T9525	41058-41061	CC	denotes	and
T9526	41062-41066	NN	denotes	part
T9527	41067-41069	IN	denotes	of
T9528	41070-41074	NN	denotes	exon
T9529	41075-41076	CD	denotes	5
T9530	41077-41081	IN	denotes	with
T9531	41082-41083	DT	denotes	a
T9533	41084-41092	NN	denotes	neomycin
T9535	41092-41093	HYPH	denotes	-
T9534	41093-41102	JJ	denotes	resistant
T9536	41103-41107	NN	denotes	gene
T9532	41108-41116	NN	denotes	cassette
T9537	41116-41117	.	denotes	.
T9538	41117-41274	sentence	denotes	An SalI site was introduced at the 3′ end of the 3-kb DNA fragment and was used to linearize the plasmid for electroporation into embryonic stem (ES) cells.
T9539	41118-41120	DT	denotes	An
T9541	41121-41125	NN	denotes	SalI
T9540	41126-41130	NN	denotes	site
T9543	41131-41134	VBD	denotes	was
T9542	41135-41145	VBN	denotes	introduced
T9544	41146-41148	IN	denotes	at
T9545	41149-41152	DT	denotes	the
T9547	41153-41154	CD	denotes	3
T9548	41154-41155	SYM	denotes	′
T9546	41156-41159	NN	denotes	end
T9549	41160-41162	IN	denotes	of
T9550	41163-41166	DT	denotes	the
T9552	41167-41168	CD	denotes	3
T9554	41168-41169	HYPH	denotes	-
T9553	41169-41171	NN	denotes	kb
T9555	41172-41175	NN	denotes	DNA
T9551	41176-41184	NN	denotes	fragment
T9556	41185-41188	CC	denotes	and
T9557	41189-41192	VBD	denotes	was
T9558	41193-41197	VBN	denotes	used
T9559	41198-41200	TO	denotes	to
T9560	41201-41210	VB	denotes	linearize
T9561	41211-41214	DT	denotes	the
T9562	41215-41222	NN	denotes	plasmid
T9563	41223-41226	IN	denotes	for
T9564	41227-41242	NN	denotes	electroporation
T9565	41243-41247	IN	denotes	into
T9566	41248-41257	JJ	denotes	embryonic
T9567	41258-41262	NN	denotes	stem
T9569	41263-41264	-LRB-	denotes	(
T9570	41264-41266	NN	denotes	ES
T9571	41266-41267	-RRB-	denotes	)
T9568	41268-41273	NNS	denotes	cells
T9572	41273-41274	.	denotes	.
T9573	41274-41427	sentence	denotes	Approximately 1,000 ES colonies were screened and two clones were identified that contained the Sam68 mutant allele, as determined by Southern blotting.
T9574	41275-41288	RB	denotes	Approximately
T9575	41289-41294	CD	denotes	1,000
T9577	41295-41297	NN	denotes	ES
T9576	41298-41306	NNS	denotes	colonies
T9579	41307-41311	VBD	denotes	were
T9578	41312-41320	VBN	denotes	screened
T9580	41321-41324	CC	denotes	and
T9581	41325-41328	CD	denotes	two
T9582	41329-41335	NNS	denotes	clones
T9584	41336-41340	VBD	denotes	were
T9583	41341-41351	VBN	denotes	identified
T9585	41352-41356	WDT	denotes	that
T9586	41357-41366	VBD	denotes	contained
T9587	41367-41370	DT	denotes	the
T9588	41371-41376	NN	denotes	Sam68
T9590	41377-41383	NN	denotes	mutant
T9589	41384-41390	NN	denotes	allele
T9591	41390-41392	,	denotes	,
T9592	41392-41394	IN	denotes	as
T9593	41395-41405	VBN	denotes	determined
T9594	41406-41408	IN	denotes	by
T9595	41409-41417	NNP	denotes	Southern
T9596	41418-41426	NN	denotes	blotting
T9597	41426-41427	.	denotes	.
T9598	41427-41620	sentence	denotes	Targeted ES cells were injected into 3.5-day-old BALB/c blastocysts and were transferred into CD-1 foster mothers, and animals classified as chimeras by coat color were mated with BALB/c mice.
T9599	41428-41436	VBN	denotes	Targeted
T9601	41437-41439	NN	denotes	ES
T9600	41440-41445	NNS	denotes	cells
T9603	41446-41450	VBD	denotes	were
T9602	41451-41459	VBN	denotes	injected
T9604	41460-41464	IN	denotes	into
T9605	41465-41468	CD	denotes	3.5
T9607	41468-41469	HYPH	denotes	-
T9606	41469-41472	NN	denotes	day
T9609	41472-41473	HYPH	denotes	-
T9608	41473-41476	JJ	denotes	old
T9611	41477-41481	NN	denotes	BALB
T9613	41481-41482	HYPH	denotes	/
T9612	41482-41483	NN	denotes	c
T9610	41484-41495	NNS	denotes	blastocysts
T9614	41496-41499	CC	denotes	and
T9615	41500-41504	VBD	denotes	were
T9616	41505-41516	VBN	denotes	transferred
T9617	41517-41521	IN	denotes	into
T9618	41522-41524	NN	denotes	CD
T9620	41524-41525	HYPH	denotes	-
T9621	41525-41526	CD	denotes	1
T9622	41527-41533	NN	denotes	foster
T9619	41534-41541	NNS	denotes	mothers
T9623	41541-41543	,	denotes	,
T9624	41543-41546	CC	denotes	and
T9625	41547-41554	NNS	denotes	animals
T9627	41555-41565	VBN	denotes	classified
T9628	41566-41568	IN	denotes	as
T9629	41569-41577	NNS	denotes	chimeras
T9630	41578-41580	IN	denotes	by
T9631	41581-41585	NN	denotes	coat
T9632	41586-41591	NN	denotes	color
T9633	41592-41596	VBD	denotes	were
T9626	41597-41602	VBN	denotes	mated
T9634	41603-41607	IN	denotes	with
T9635	41608-41612	NN	denotes	BALB
T9637	41612-41613	HYPH	denotes	/
T9636	41613-41614	NN	denotes	c
T9638	41615-41619	NNS	denotes	mice
T9639	41619-41620	.	denotes	.
T9640	41620-41708	sentence	denotes	Germ line transmission was achieved and the mice were maintained in C57BL/6 background.
T9641	41621-41625	NN	denotes	Germ
T9642	41626-41630	NN	denotes	line
T9643	41631-41643	NN	denotes	transmission
T9645	41644-41647	VBD	denotes	was
T9644	41648-41656	VBN	denotes	achieved
T9646	41657-41660	CC	denotes	and
T9647	41661-41664	DT	denotes	the
T9648	41665-41669	NNS	denotes	mice
T9650	41670-41674	VBD	denotes	were
T9649	41675-41685	VBN	denotes	maintained
T9651	41686-41688	IN	denotes	in
T9652	41689-41694	NN	denotes	C57BL
T9654	41694-41695	HYPH	denotes	/
T9655	41695-41696	CD	denotes	6
T9653	41697-41707	NN	denotes	background
T9656	41707-41708	.	denotes	.
T9657	41708-41914	sentence	denotes	The mice used for this study represent mice that were backcrossed in C57BL/6 between three and eight generations; in addition, we maintained the mice in the 129/SvJ strain and observed a similar phenotype.
T9658	41709-41712	DT	denotes	The
T9659	41713-41717	NNS	denotes	mice
T9661	41718-41722	VBN	denotes	used
T9662	41723-41726	IN	denotes	for
T9663	41727-41731	DT	denotes	this
T9664	41732-41737	NN	denotes	study
T9660	41738-41747	VBP	denotes	represent
T9666	41748-41752	NNS	denotes	mice
T9667	41753-41757	WDT	denotes	that
T9669	41758-41762	VBD	denotes	were
T9668	41763-41774	VBN	denotes	backcrossed
T9670	41775-41777	IN	denotes	in
T9671	41778-41783	NN	denotes	C57BL
T9672	41783-41784	HYPH	denotes	/
T9673	41784-41785	CD	denotes	6
T9674	41786-41793	IN	denotes	between
T9675	41794-41799	CD	denotes	three
T9677	41800-41803	CC	denotes	and
T9678	41804-41809	CD	denotes	eight
T9676	41810-41821	NNS	denotes	generations
T9679	41821-41822	:	denotes	;
T9680	41823-41825	IN	denotes	in
T9681	41826-41834	NN	denotes	addition
T9682	41834-41836	,	denotes	,
T9683	41836-41838	PRP	denotes	we
T9665	41839-41849	VBD	denotes	maintained
T9684	41850-41853	DT	denotes	the
T9685	41854-41858	NNS	denotes	mice
T9686	41859-41861	IN	denotes	in
T9687	41862-41865	DT	denotes	the
T9689	41866-41869	CD	denotes	129
T9691	41869-41870	HYPH	denotes	/
T9690	41870-41873	NN	denotes	SvJ
T9688	41874-41880	NN	denotes	strain
T9692	41881-41884	CC	denotes	and
T9693	41885-41893	VBD	denotes	observed
T9694	41894-41895	DT	denotes	a
T9696	41896-41903	JJ	denotes	similar
T9695	41904-41913	NN	denotes	phenotype
T9697	41913-41914	.	denotes	.
T9732	41916-41926	NN	denotes	Genotyping
T9733	41927-41930	CC	denotes	and
T9734	41931-41941	NN	denotes	immunoblot
T9735	41942-41950	NNS	denotes	analyses
T9736	41950-41951	.	denotes	.
T9737	41951-42090	sentence	denotes	All mouse procedures were performed in accordance with McGill University guidelines, which are set by the Canadian Council on Animal Care.
T9738	41952-41955	DT	denotes	All
T9740	41956-41961	NN	denotes	mouse
T9739	41962-41972	NNS	denotes	procedures
T9742	41973-41977	VBD	denotes	were
T9741	41978-41987	VBN	denotes	performed
T9743	41988-41990	IN	denotes	in
T9744	41991-42001	NN	denotes	accordance
T9745	42002-42006	IN	denotes	with
T9746	42007-42013	NNP	denotes	McGill
T9747	42014-42024	NNP	denotes	University
T9748	42025-42035	NNS	denotes	guidelines
T9749	42035-42037	,	denotes	,
T9750	42037-42042	WDT	denotes	which
T9752	42043-42046	VBP	denotes	are
T9751	42047-42050	VBN	denotes	set
T9753	42051-42053	IN	denotes	by
T9754	42054-42057	DT	denotes	the
T9756	42058-42066	NNP	denotes	Canadian
T9755	42067-42074	NNP	denotes	Council
T9757	42075-42077	IN	denotes	on
T9758	42078-42084	NNP	denotes	Animal
T9759	42085-42089	NNP	denotes	Care
T9760	42089-42090	.	denotes	.
T9761	42090-42194	sentence	denotes	Genomic DNA was isolated from tail biopsies and analyzed by Southern blotting and genomic PCR analysis.
T9762	42091-42098	JJ	denotes	Genomic
T9763	42099-42102	NN	denotes	DNA
T9765	42103-42106	VBD	denotes	was
T9764	42107-42115	VBN	denotes	isolated
T9766	42116-42120	IN	denotes	from
T9767	42121-42125	NN	denotes	tail
T9768	42126-42134	NNS	denotes	biopsies
T9769	42135-42138	CC	denotes	and
T9770	42139-42147	VBN	denotes	analyzed
T9771	42148-42150	IN	denotes	by
T9772	42151-42159	NNP	denotes	Southern
T9773	42160-42168	NN	denotes	blotting
T9774	42169-42172	CC	denotes	and
T9775	42173-42180	JJ	denotes	genomic
T9777	42181-42184	NN	denotes	PCR
T9776	42185-42193	NN	denotes	analysis
T9778	42193-42194	.	denotes	.
T9779	42194-42396	sentence	denotes	The DNA fragment utilized as the probe for the Southern blotting analysis was amplified with the following two oligonucleotides (5′-AAG CCT TTA CTG GTT GTG T-3′) and (5′-CTT GAA ACG CAC CGT AGG CT-3′).
T9780	42195-42198	DT	denotes	The
T9782	42199-42202	NN	denotes	DNA
T9781	42203-42211	NN	denotes	fragment
T9784	42212-42220	VBN	denotes	utilized
T9785	42221-42223	IN	denotes	as
T9786	42224-42227	DT	denotes	the
T9787	42228-42233	NN	denotes	probe
T9788	42234-42237	IN	denotes	for
T9789	42238-42241	DT	denotes	the
T9791	42242-42250	NNP	denotes	Southern
T9792	42251-42259	NN	denotes	blotting
T9790	42260-42268	NN	denotes	analysis
T9793	42269-42272	VBD	denotes	was
T9783	42273-42282	VBN	denotes	amplified
T9794	42283-42287	IN	denotes	with
T9795	42288-42291	DT	denotes	the
T9797	42292-42301	VBG	denotes	following
T9798	42302-42305	CD	denotes	two
T9796	42306-42322	NNS	denotes	oligonucleotides
T9799	42323-42324	-LRB-	denotes	(
T9800	42324-42325	CD	denotes	5
T9801	42325-42326	SYM	denotes	′
T9802	42326-42327	HYPH	denotes	-
T9803	42327-42330	NN	denotes	AAG
T9805	42331-42334	NN	denotes	CCT
T9806	42335-42338	NN	denotes	TTA
T9807	42339-42342	NN	denotes	CTG
T9808	42343-42346	NN	denotes	GTT
T9809	42347-42350	NN	denotes	GTG
T9804	42351-42352	NN	denotes	T
T9810	42352-42353	HYPH	denotes	-
T9811	42353-42354	CD	denotes	3
T9812	42354-42355	SYM	denotes	′
T9813	42355-42356	-RRB-	denotes	)
T9814	42357-42360	CC	denotes	and
T9815	42361-42362	-LRB-	denotes	(
T9816	42362-42363	CD	denotes	5
T9817	42363-42364	SYM	denotes	′
T9818	42364-42365	HYPH	denotes	-
T9819	42365-42368	NN	denotes	CTT
T9821	42369-42372	NN	denotes	GAA
T9822	42373-42376	NN	denotes	ACG
T9823	42377-42380	NN	denotes	CAC
T9824	42381-42384	NN	denotes	CGT
T9825	42385-42388	NN	denotes	AGG
T9820	42389-42391	NN	denotes	CT
T9826	42391-42392	HYPH	denotes	-
T9827	42392-42393	CD	denotes	3
T9828	42393-42394	SYM	denotes	′
T9829	42394-42395	-RRB-	denotes	)
T9830	42395-42396	.	denotes	.
T9831	42396-42570	sentence	denotes	The wild-type sam68 allele was identified by genomic PCR using the following oligonucleotides 5′-AAA TCC TAA CCC TCC TCA GTC AG-3′ and 5′-GAT ATG ATG GAT GAT ATC TGT CAG-3′.
T9832	42397-42400	DT	denotes	The
T9834	42401-42405	JJ	denotes	wild
T9836	42405-42406	HYPH	denotes	-
T9835	42406-42410	NN	denotes	type
T9837	42411-42416	NN	denotes	sam68
T9833	42417-42423	NN	denotes	allele
T9839	42424-42427	VBD	denotes	was
T9838	42428-42438	VBN	denotes	identified
T9840	42439-42441	IN	denotes	by
T9841	42442-42449	JJ	denotes	genomic
T9842	42450-42453	NN	denotes	PCR
T9843	42454-42459	VBG	denotes	using
T9844	42460-42463	DT	denotes	the
T9846	42464-42473	VBG	denotes	following
T9845	42474-42490	NNS	denotes	oligonucleotides
T9847	42491-42492	CD	denotes	5
T9848	42492-42493	SYM	denotes	′
T9849	42493-42494	HYPH	denotes	-
T9850	42494-42497	NN	denotes	AAA
T9852	42498-42501	NN	denotes	TCC
T9853	42502-42505	NN	denotes	TAA
T9854	42506-42509	NN	denotes	CCC
T9855	42510-42513	NN	denotes	TCC
T9856	42514-42517	NN	denotes	TCA
T9857	42518-42521	NN	denotes	GTC
T9851	42522-42524	NN	denotes	AG
T9858	42524-42525	HYPH	denotes	-
T9859	42525-42526	CD	denotes	3
T9860	42526-42527	SYM	denotes	′
T9861	42528-42531	CC	denotes	and
T9862	42532-42533	CD	denotes	5
T9863	42533-42534	SYM	denotes	′
T9864	42534-42535	HYPH	denotes	-
T9865	42535-42538	NN	denotes	GAT
T9867	42539-42542	NN	denotes	ATG
T9868	42543-42546	NN	denotes	ATG
T9869	42547-42550	NN	denotes	GAT
T9870	42551-42554	NN	denotes	GAT
T9871	42555-42558	NN	denotes	ATC
T9872	42559-42562	NN	denotes	TGT
T9866	42563-42566	NN	denotes	CAG
T9873	42566-42567	HYPH	denotes	-
T9874	42567-42568	CD	denotes	3
T9875	42568-42569	SYM	denotes	′
T9876	42569-42570	.	denotes	.
T9877	42570-42738	sentence	denotes	The sam68-targeted allele was identified by genomic PCR using the following oligonucleotides 5′-CTT GGG TGG AGA GGC TAT TCG-3′ and 5′-GTC GGG CAT GCG CGC CTT GAG C-3′.
T9878	42571-42574	DT	denotes	The
T9880	42575-42580	NN	denotes	sam68
T9882	42580-42581	HYPH	denotes	-
T9881	42581-42589	VBN	denotes	targeted
T9879	42590-42596	NN	denotes	allele
T9884	42597-42600	VBD	denotes	was
T9883	42601-42611	VBN	denotes	identified
T9885	42612-42614	IN	denotes	by
T9886	42615-42622	JJ	denotes	genomic
T9887	42623-42626	NN	denotes	PCR
T9888	42627-42632	VBG	denotes	using
T9889	42633-42636	DT	denotes	the
T9891	42637-42646	VBG	denotes	following
T9890	42647-42663	NNS	denotes	oligonucleotides
T9892	42664-42665	CD	denotes	5
T9893	42665-42666	SYM	denotes	′
T9894	42666-42667	HYPH	denotes	-
T9895	42667-42670	NN	denotes	CTT
T9897	42671-42674	NN	denotes	GGG
T9898	42675-42678	NN	denotes	TGG
T9899	42679-42682	NN	denotes	AGA
T9900	42683-42686	NN	denotes	GGC
T9901	42687-42690	NN	denotes	TAT
T9896	42691-42694	NN	denotes	TCG
T9902	42694-42695	HYPH	denotes	-
T9903	42695-42696	CD	denotes	3
T9904	42696-42697	SYM	denotes	′
T9905	42698-42701	CC	denotes	and
T9906	42702-42703	CD	denotes	5
T9907	42703-42704	SYM	denotes	′
T9908	42704-42705	HYPH	denotes	-
T9909	42705-42708	NN	denotes	GTC
T9911	42709-42712	NN	denotes	GGG
T9912	42713-42716	NN	denotes	CAT
T9913	42717-42720	NN	denotes	GCG
T9914	42721-42724	NN	denotes	CGC
T9915	42725-42728	NN	denotes	CTT
T9916	42729-42732	NN	denotes	GAG
T9910	42733-42734	NN	denotes	C
T9917	42734-42735	HYPH	denotes	-
T9918	42735-42736	CD	denotes	3
T9919	42736-42737	SYM	denotes	′
T9920	42737-42738	.	denotes	.
T9978	42740-42749	NN	denotes	Radiology
T9979	42750-42753	CC	denotes	and
T9980	42754-42759	NN	denotes	serum
T9981	42760-42772	NN	denotes	biochemistry
T9982	42773-42775	IN	denotes	on
T9983	42776-42781	NN	denotes	Sam68
T9985	42781-42782	SYM	denotes	+
T9986	42782-42783	HYPH	denotes	/
T9987	42783-42784	SYM	denotes	+
T9988	42785-42788	CC	denotes	and
T9989	42789-42794	NN	denotes	Sam68
T9990	42794-42795	SYM	denotes	−
T9991	42795-42796	HYPH	denotes	/
T9992	42796-42797	SYM	denotes	−
T9984	42798-42802	NNS	denotes	mice
T9993	42802-42803	.	denotes	.
T9994	42803-42891	sentence	denotes	Radiography, BMD, and micro-CT were performed essentially as described previously [63].
T9995	42804-42815	NN	denotes	Radiography
T9997	42815-42817	,	denotes	,
T9998	42817-42820	NN	denotes	BMD
T9999	42820-42822	,	denotes	,
T10000	42822-42825	CC	denotes	and
T10001	42826-42834	NN	denotes	micro-CT
T10002	42835-42839	VBD	denotes	were
T9996	42840-42849	VBN	denotes	performed
T10003	42850-42861	RB	denotes	essentially
T10005	42862-42864	IN	denotes	as
T10004	42865-42874	VBN	denotes	described
T10006	42875-42885	RB	denotes	previously
T10007	42886-42887	-LRB-	denotes	[
T10008	42887-42889	CD	denotes	63
T10009	42889-42890	-RRB-	denotes	]
T10010	42890-42891	.	denotes	.
T10011	42891-43098	sentence	denotes	Mice were administered a lethal dose of anesthetic at the indicated times, exsanguinated, and imaged using a Faxitron MX20 equipped with an FPX-2 Imaging system (Dalsa Medoptics, Waterloo, Ontario, Canada).
T10012	42892-42896	NNS	denotes	Mice
T10014	42897-42901	VBD	denotes	were
T10013	42902-42914	VBN	denotes	administered
T10015	42915-42916	DT	denotes	a
T10017	42917-42923	JJ	denotes	lethal
T10016	42924-42928	NN	denotes	dose
T10018	42929-42931	IN	denotes	of
T10019	42932-42942	JJ	denotes	anesthetic
T10020	42943-42945	IN	denotes	at
T10021	42946-42949	DT	denotes	the
T10023	42950-42959	VBN	denotes	indicated
T10022	42960-42965	NNS	denotes	times
T10024	42965-42967	,	denotes	,
T10025	42967-42980	VBN	denotes	exsanguinated
T10026	42980-42982	,	denotes	,
T10027	42982-42985	CC	denotes	and
T10028	42986-42992	VBN	denotes	imaged
T10029	42993-42998	VBG	denotes	using
T10030	42999-43000	DT	denotes	a
T10032	43001-43009	NNP	denotes	Faxitron
T10031	43010-43014	NNP	denotes	MX20
T10033	43015-43023	VBN	denotes	equipped
T10034	43024-43028	IN	denotes	with
T10035	43029-43031	DT	denotes	an
T10037	43032-43035	NN	denotes	FPX
T10038	43035-43036	HYPH	denotes	-
T10039	43036-43037	CD	denotes	2
T10040	43038-43045	NN	denotes	Imaging
T10036	43046-43052	NN	denotes	system
T10041	43053-43054	-LRB-	denotes	(
T10043	43054-43059	NNP	denotes	Dalsa
T10042	43060-43069	NNP	denotes	Medoptics
T10044	43069-43071	,	denotes	,
T10045	43071-43079	NNP	denotes	Waterloo
T10046	43079-43081	,	denotes	,
T10047	43081-43088	NNP	denotes	Ontario
T10048	43088-43090	,	denotes	,
T10049	43090-43096	NNP	denotes	Canada
T10050	43096-43097	-RRB-	denotes	)
T10051	43097-43098	.	denotes	.
T10052	43098-43210	sentence	denotes	Body fat, BMC, and BMD were evaluated using a Lunar PixiMUS 1.46 (GE-Lunar, Madison, Wisconsin, United States).
T10053	43099-43103	NN	denotes	Body
T10054	43104-43107	NN	denotes	fat
T10056	43107-43109	,	denotes	,
T10057	43109-43112	NN	denotes	BMC
T10058	43112-43114	,	denotes	,
T10059	43114-43117	CC	denotes	and
T10060	43118-43121	NN	denotes	BMD
T10061	43122-43126	VBD	denotes	were
T10055	43127-43136	VBN	denotes	evaluated
T10062	43137-43142	VBG	denotes	using
T10063	43143-43144	DT	denotes	a
T10065	43145-43150	NNP	denotes	Lunar
T10064	43151-43158	NNP	denotes	PixiMUS
T10066	43159-43163	CD	denotes	1.46
T10067	43164-43165	-LRB-	denotes	(
T10069	43165-43167	NNP	denotes	GE
T10070	43167-43168	HYPH	denotes	-
T10068	43168-43173	NNP	denotes	Lunar
T10071	43173-43175	,	denotes	,
T10072	43175-43182	NNP	denotes	Madison
T10073	43182-43184	,	denotes	,
T10074	43184-43193	NNP	denotes	Wisconsin
T10075	43193-43195	,	denotes	,
T10076	43195-43201	NNP	denotes	United
T10077	43202-43208	NNP	denotes	States
T10078	43208-43209	-RRB-	denotes	)
T10079	43209-43210	.	denotes	.
T10080	43210-43351	sentence	denotes	Morphometric parameters were determined on anesthetized mice at the time of sacrifice by direct measurement or from the Faxitron radiograph.
T10081	43211-43223	JJ	denotes	Morphometric
T10082	43224-43234	NNS	denotes	parameters
T10084	43235-43239	VBD	denotes	were
T10083	43240-43250	VBN	denotes	determined
T10085	43251-43253	IN	denotes	on
T10086	43254-43266	VBN	denotes	anesthetized
T10087	43267-43271	NNS	denotes	mice
T10088	43272-43274	IN	denotes	at
T10089	43275-43278	DT	denotes	the
T10090	43279-43283	NN	denotes	time
T10091	43284-43286	IN	denotes	of
T10092	43287-43296	NN	denotes	sacrifice
T10093	43297-43299	IN	denotes	by
T10094	43300-43306	JJ	denotes	direct
T10095	43307-43318	NN	denotes	measurement
T10096	43319-43321	CC	denotes	or
T10097	43322-43326	IN	denotes	from
T10098	43327-43330	DT	denotes	the
T10100	43331-43339	NNP	denotes	Faxitron
T10099	43340-43350	NN	denotes	radiograph
T10101	43350-43351	.	denotes	.
T10102	43351-43496	sentence	denotes	Micro-CT was performed on the left femur and fourth lumbar vertebra after removal of soft tissues and overnight fixation in 4% paraformaldehyde.
T10103	43352-43360	NN	denotes	Micro-CT
T10105	43361-43364	VBD	denotes	was
T10104	43365-43374	VBN	denotes	performed
T10106	43375-43377	IN	denotes	on
T10107	43378-43381	DT	denotes	the
T10109	43382-43386	JJ	denotes	left
T10108	43387-43392	NN	denotes	femur
T10110	43393-43396	CC	denotes	and
T10111	43397-43403	JJ	denotes	fourth
T10113	43404-43410	NN	denotes	lumbar
T10112	43411-43419	NN	denotes	vertebra
T10114	43420-43425	IN	denotes	after
T10115	43426-43433	NN	denotes	removal
T10116	43434-43436	IN	denotes	of
T10117	43437-43441	JJ	denotes	soft
T10118	43442-43449	NNS	denotes	tissues
T10119	43450-43453	CC	denotes	and
T10120	43454-43463	JJ	denotes	overnight
T10121	43464-43472	NN	denotes	fixation
T10122	43473-43475	IN	denotes	in
T10123	43476-43477	CD	denotes	4
T10124	43477-43478	NN	denotes	%
T10125	43479-43495	NN	denotes	paraformaldehyde
T10126	43495-43496	.	denotes	.
T10127	43496-43599	sentence	denotes	The distal metaphysis was scanned with a Skyscan 1072 micro-CT instrument (Skyscan, Antwerp, Belgium).
T10128	43497-43500	DT	denotes	The
T10130	43501-43507	JJ	denotes	distal
T10129	43508-43518	NN	denotes	metaphysis
T10132	43519-43522	VBD	denotes	was
T10131	43523-43530	VBN	denotes	scanned
T10133	43531-43535	IN	denotes	with
T10134	43536-43537	DT	denotes	a
T10136	43538-43545	NNP	denotes	Skyscan
T10137	43546-43550	CD	denotes	1072
T10138	43551-43559	NN	denotes	micro-CT
T10135	43560-43570	NN	denotes	instrument
T10139	43571-43572	-LRB-	denotes	(
T10140	43572-43579	NNP	denotes	Skyscan
T10141	43579-43581	,	denotes	,
T10142	43581-43588	NNP	denotes	Antwerp
T10143	43588-43590	,	denotes	,
T10144	43590-43597	NNP	denotes	Belgium
T10145	43597-43598	-RRB-	denotes	)
T10146	43598-43599	.	denotes	.
T10147	43599-43689	sentence	denotes	Image acquisition was performed at 100 kV and 98 μA, with a 0.9° rotation between frames.
T10148	43600-43605	NN	denotes	Image
T10149	43606-43617	NN	denotes	acquisition
T10151	43618-43621	VBD	denotes	was
T10150	43622-43631	VBN	denotes	performed
T10152	43632-43634	IN	denotes	at
T10153	43635-43638	CD	denotes	100
T10154	43639-43641	NN	denotes	kV
T10155	43642-43645	CC	denotes	and
T10156	43646-43648	CD	denotes	98
T10157	43649-43651	NN	denotes	μA
T10158	43651-43653	,	denotes	,
T10159	43653-43657	IN	denotes	with
T10160	43658-43659	DT	denotes	a
T10162	43660-43663	CD	denotes	0.9
T10163	43663-43664	NN	denotes	°
T10161	43665-43673	NN	denotes	rotation
T10164	43674-43681	IN	denotes	between
T10165	43682-43688	NNS	denotes	frames
T10166	43688-43689	.	denotes	.
T10167	43689-43859	sentence	denotes	The two-dimensional images were used to generate three-dimensional reconstructions to obtain quantitative data with the 3D Creator software supplied with the instrument.
T10168	43690-43693	DT	denotes	The
T10170	43694-43697	CD	denotes	two
T10172	43697-43698	HYPH	denotes	-
T10171	43698-43709	JJ	denotes	dimensional
T10169	43710-43716	NNS	denotes	images
T10174	43717-43721	VBD	denotes	were
T10173	43722-43726	VBN	denotes	used
T10175	43727-43729	TO	denotes	to
T10176	43730-43738	VB	denotes	generate
T10177	43739-43744	CD	denotes	three
T10179	43744-43745	HYPH	denotes	-
T10178	43745-43756	JJ	denotes	dimensional
T10180	43757-43772	NNS	denotes	reconstructions
T10181	43773-43775	TO	denotes	to
T10182	43776-43782	VB	denotes	obtain
T10183	43783-43795	JJ	denotes	quantitative
T10184	43796-43800	NNS	denotes	data
T10185	43801-43805	IN	denotes	with
T10186	43806-43809	DT	denotes	the
T10188	43810-43812	JJ	denotes	3D
T10189	43813-43820	NN	denotes	Creator
T10187	43821-43829	NN	denotes	software
T10190	43830-43838	VBN	denotes	supplied
T10191	43839-43843	IN	denotes	with
T10192	43844-43847	DT	denotes	the
T10193	43848-43858	NN	denotes	instrument
T10194	43858-43859	.	denotes	.
T10195	43859-44024	sentence	denotes	Serum biochemistry (ALP, Ca, PO4, Mg) was determined at the Rodent Diagnostics Lab (McGill University, Montréal, Quebec, Canada) using routine automated techniques.
T10196	43860-43865	NN	denotes	Serum
T10197	43866-43878	NN	denotes	biochemistry
T10199	43879-43880	-LRB-	denotes	(
T10201	43880-43883	NN	denotes	ALP
T10202	43883-43885	,	denotes	,
T10203	43885-43887	NN	denotes	Ca
T10204	43887-43889	,	denotes	,
T10205	43889-43892	NN	denotes	PO4
T10206	43892-43894	,	denotes	,
T10200	43894-43896	NN	denotes	Mg
T10207	43896-43897	-RRB-	denotes	)
T10208	43898-43901	VBD	denotes	was
T10198	43902-43912	VBN	denotes	determined
T10209	43913-43915	IN	denotes	at
T10210	43916-43919	DT	denotes	the
T10212	43920-43926	NNP	denotes	Rodent
T10213	43927-43938	NNP	denotes	Diagnostics
T10211	43939-43942	NNP	denotes	Lab
T10214	43943-43944	-LRB-	denotes	(
T10216	43944-43950	NNP	denotes	McGill
T10215	43951-43961	NNP	denotes	University
T10217	43961-43963	,	denotes	,
T10218	43963-43971	NNP	denotes	Montréal
T10219	43971-43973	,	denotes	,
T10220	43973-43979	NNP	denotes	Quebec
T10221	43979-43981	,	denotes	,
T10222	43981-43987	NNP	denotes	Canada
T10223	43987-43988	-RRB-	denotes	)
T10224	43989-43994	VBG	denotes	using
T10225	43995-44002	JJ	denotes	routine
T10227	44003-44012	VBN	denotes	automated
T10226	44013-44023	NNS	denotes	techniques
T10228	44023-44024	.	denotes	.
T10229	44024-44276	sentence	denotes	Commercial assays were used to determine serum levels of CTX (RatLaps ELISA, Nordic Bioscience), 17β estradiol (IBL Immuno-Biological, Hamburg, Germany), leptin (R & D Systems, Minneapolis, Minnesota, United States), and interleukin-6 (R & D Systems).
T10230	44025-44035	JJ	denotes	Commercial
T10231	44036-44042	NNS	denotes	assays
T10233	44043-44047	VBD	denotes	were
T10232	44048-44052	VBN	denotes	used
T10234	44053-44055	TO	denotes	to
T10235	44056-44065	VB	denotes	determine
T10236	44066-44071	NN	denotes	serum
T10237	44072-44078	NNS	denotes	levels
T10238	44079-44081	IN	denotes	of
T10239	44082-44085	NN	denotes	CTX
T10240	44086-44087	-LRB-	denotes	(
T10242	44087-44094	NNP	denotes	RatLaps
T10241	44095-44100	NNP	denotes	ELISA
T10243	44100-44102	,	denotes	,
T10244	44102-44108	NNP	denotes	Nordic
T10245	44109-44119	NNP	denotes	Bioscience
T10246	44119-44120	-RRB-	denotes	)
T10247	44120-44122	,	denotes	,
T10248	44122-44125	CD	denotes	17β
T10249	44126-44135	NN	denotes	estradiol
T10250	44136-44137	-LRB-	denotes	(
T10252	44137-44140	NNP	denotes	IBL
T10251	44141-44158	NNP	denotes	Immuno-Biological
T10253	44158-44160	,	denotes	,
T10254	44160-44167	NNP	denotes	Hamburg
T10255	44167-44169	,	denotes	,
T10256	44169-44176	NNP	denotes	Germany
T10257	44176-44177	-RRB-	denotes	)
T10258	44177-44179	,	denotes	,
T10259	44179-44185	NN	denotes	leptin
T10260	44186-44187	-LRB-	denotes	(
T10262	44187-44188	NNP	denotes	R
T10263	44189-44190	CC	denotes	&
T10264	44191-44192	NNP	denotes	D
T10261	44193-44200	NNP	denotes	Systems
T10265	44200-44202	,	denotes	,
T10266	44202-44213	NNP	denotes	Minneapolis
T10267	44213-44215	,	denotes	,
T10268	44215-44224	NNP	denotes	Minnesota
T10269	44224-44226	,	denotes	,
T10270	44226-44232	NNP	denotes	United
T10271	44233-44239	NNP	denotes	States
T10272	44239-44240	-RRB-	denotes	)
T10273	44240-44242	,	denotes	,
T10274	44242-44245	CC	denotes	and
T10275	44246-44257	NN	denotes	interleukin
T10276	44257-44258	HYPH	denotes	-
T10277	44258-44259	CD	denotes	6
T10278	44260-44261	-LRB-	denotes	(
T10280	44261-44262	NNP	denotes	R
T10281	44263-44264	CC	denotes	&
T10282	44265-44266	NNP	denotes	D
T10279	44267-44274	NNP	denotes	Systems
T10283	44274-44275	-RRB-	denotes	)
T10284	44275-44276	.	denotes	.
T10388	44278-44280	FW	denotes	Ex
T10389	44281-44285	FW	denotes	vivo
T10390	44286-44296	NN	denotes	assessment
T10391	44297-44299	IN	denotes	of
T10392	44300-44310	NN	denotes	osteoblast
T10394	44311-44314	CC	denotes	and
T10395	44315-44325	NN	denotes	osteoclast
T10393	44326-44334	NN	denotes	activity
T10396	44334-44335	.	denotes	.
T10397	44335-44542	sentence	denotes	Bone marrow was flushed from the tibia and femora of juvenile 4-week-old Sam68+/+ and Sam68−/− mice to obtain stromal cells that were maintained in differentiation medium for 6 or 18 days as described [63].
T10398	44336-44340	NN	denotes	Bone
T10399	44341-44347	NN	denotes	marrow
T10401	44348-44351	VBD	denotes	was
T10400	44352-44359	VBN	denotes	flushed
T10402	44360-44364	IN	denotes	from
T10403	44365-44368	DT	denotes	the
T10404	44369-44374	NN	denotes	tibia
T10405	44375-44378	CC	denotes	and
T10406	44379-44385	NNS	denotes	femora
T10407	44386-44388	IN	denotes	of
T10408	44389-44397	JJ	denotes	juvenile
T10410	44398-44399	CD	denotes	4
T10412	44399-44400	HYPH	denotes	-
T10411	44400-44404	NN	denotes	week
T10414	44404-44405	HYPH	denotes	-
T10413	44405-44408	JJ	denotes	old
T10415	44409-44414	NN	denotes	Sam68
T10416	44414-44415	SYM	denotes	+
T10417	44415-44416	HYPH	denotes	/
T10418	44416-44417	SYM	denotes	+
T10419	44418-44421	CC	denotes	and
T10420	44422-44427	NN	denotes	Sam68
T10421	44427-44428	SYM	denotes	−
T10422	44428-44429	HYPH	denotes	/
T10423	44429-44430	SYM	denotes	−
T10409	44431-44435	NNS	denotes	mice
T10424	44436-44438	TO	denotes	to
T10425	44439-44445	VB	denotes	obtain
T10426	44446-44453	JJ	denotes	stromal
T10427	44454-44459	NNS	denotes	cells
T10428	44460-44464	WDT	denotes	that
T10430	44465-44469	VBD	denotes	were
T10429	44470-44480	VBN	denotes	maintained
T10431	44481-44483	IN	denotes	in
T10432	44484-44499	NN	denotes	differentiation
T10433	44500-44506	NN	denotes	medium
T10434	44507-44510	IN	denotes	for
T10435	44511-44512	CD	denotes	6
T10437	44513-44515	CC	denotes	or
T10438	44516-44518	CD	denotes	18
T10436	44519-44523	NNS	denotes	days
T10439	44524-44526	IN	denotes	as
T10440	44527-44536	VBN	denotes	described
T10441	44537-44538	-LRB-	denotes	[
T10442	44538-44540	CD	denotes	63
T10443	44540-44541	-RRB-	denotes	]
T10444	44541-44542	.	denotes	.
T10445	44542-44645	sentence	denotes	Cultures were stained in situ for ALP activity and with von Kossa stain to detect mineralized nodules.
T10446	44543-44551	NNS	denotes	Cultures
T10448	44552-44556	VBD	denotes	were
T10447	44557-44564	VBN	denotes	stained
T10449	44565-44567	FW	denotes	in
T10450	44568-44572	FW	denotes	situ
T10451	44573-44576	IN	denotes	for
T10452	44577-44580	NN	denotes	ALP
T10453	44581-44589	NN	denotes	activity
T10454	44590-44593	CC	denotes	and
T10455	44594-44598	IN	denotes	with
T10456	44599-44602	NNP	denotes	von
T10457	44603-44608	NNP	denotes	Kossa
T10458	44609-44614	NN	denotes	stain
T10459	44615-44617	TO	denotes	to
T10460	44618-44624	VB	denotes	detect
T10461	44625-44636	VBN	denotes	mineralized
T10462	44637-44644	NNS	denotes	nodules
T10463	44644-44645	.	denotes	.
T10464	44645-44790	sentence	denotes	Total RNA was harvested from parallel cultures for RT-PCR analysis of osteoblast-related gene expression over time as described previously [63].
T10465	44646-44651	JJ	denotes	Total
T10466	44652-44655	NN	denotes	RNA
T10468	44656-44659	VBD	denotes	was
T10467	44660-44669	VBN	denotes	harvested
T10469	44670-44674	IN	denotes	from
T10470	44675-44683	JJ	denotes	parallel
T10471	44684-44692	NNS	denotes	cultures
T10472	44693-44696	IN	denotes	for
T10473	44697-44699	NN	denotes	RT
T10475	44699-44700	HYPH	denotes	-
T10474	44700-44703	NN	denotes	PCR
T10476	44704-44712	NN	denotes	analysis
T10477	44713-44715	IN	denotes	of
T10478	44716-44726	NN	denotes	osteoblast
T10480	44726-44727	HYPH	denotes	-
T10479	44727-44734	VBN	denotes	related
T10482	44735-44739	NN	denotes	gene
T10481	44740-44750	NN	denotes	expression
T10483	44751-44755	IN	denotes	over
T10484	44756-44760	NN	denotes	time
T10485	44761-44763	IN	denotes	as
T10486	44764-44773	VBN	denotes	described
T10487	44774-44784	RB	denotes	previously
T10488	44785-44786	-LRB-	denotes	[
T10489	44786-44788	CD	denotes	63
T10490	44788-44789	-RRB-	denotes	]
T10491	44789-44790	.	denotes	.
T10492	44790-44936	sentence	denotes	Osteoclasts were isolated from the crushed long bones of juvenile Sam68+/+ and Sam68−/− mice and used for quantitative studies as described [64].
T10493	44791-44802	NNS	denotes	Osteoclasts
T10495	44803-44807	VBD	denotes	were
T10494	44808-44816	VBN	denotes	isolated
T10496	44817-44821	IN	denotes	from
T10497	44822-44825	DT	denotes	the
T10499	44826-44833	VBN	denotes	crushed
T10500	44834-44838	JJ	denotes	long
T10498	44839-44844	NNS	denotes	bones
T10501	44845-44847	IN	denotes	of
T10502	44848-44856	JJ	denotes	juvenile
T10504	44857-44862	NN	denotes	Sam68
T10505	44862-44863	SYM	denotes	+
T10506	44863-44864	HYPH	denotes	/
T10507	44864-44865	SYM	denotes	+
T10508	44866-44869	CC	denotes	and
T10509	44870-44875	NN	denotes	Sam68
T10510	44875-44876	SYM	denotes	−
T10511	44876-44877	HYPH	denotes	/
T10512	44877-44878	SYM	denotes	−
T10503	44879-44883	NNS	denotes	mice
T10513	44884-44887	CC	denotes	and
T10514	44888-44892	VBN	denotes	used
T10515	44893-44896	IN	denotes	for
T10516	44897-44909	JJ	denotes	quantitative
T10517	44910-44917	NNS	denotes	studies
T10518	44918-44920	IN	denotes	as
T10519	44921-44930	VBN	denotes	described
T10520	44931-44932	-LRB-	denotes	[
T10521	44932-44934	CD	denotes	64
T10522	44934-44935	-RRB-	denotes	]
T10523	44935-44936	.	denotes	.
T10524	44936-45089	sentence	denotes	Briefly, cells were plated on glass coverslips or on dentin slices in 24-well cluster plates for assessment of cell number and pit number, respectively.
T10525	44937-44944	RB	denotes	Briefly
T10527	44944-44946	,	denotes	,
T10528	44946-44951	NNS	denotes	cells
T10529	44952-44956	VBD	denotes	were
T10526	44957-44963	VBN	denotes	plated
T10530	44964-44966	IN	denotes	on
T10531	44967-44972	NN	denotes	glass
T10532	44973-44983	NNS	denotes	coverslips
T10533	44984-44986	CC	denotes	or
T10534	44987-44989	IN	denotes	on
T10535	44990-44996	NN	denotes	dentin
T10536	44997-45003	NNS	denotes	slices
T10537	45004-45006	IN	denotes	in
T10538	45007-45009	CD	denotes	24
T10540	45009-45010	HYPH	denotes	-
T10539	45010-45014	NN	denotes	well
T10542	45015-45022	NN	denotes	cluster
T10541	45023-45029	NNS	denotes	plates
T10543	45030-45033	IN	denotes	for
T10544	45034-45044	NN	denotes	assessment
T10545	45045-45047	IN	denotes	of
T10546	45048-45052	NN	denotes	cell
T10547	45053-45059	NN	denotes	number
T10548	45060-45063	CC	denotes	and
T10549	45064-45067	NN	denotes	pit
T10550	45068-45074	NN	denotes	number
T10551	45074-45076	,	denotes	,
T10552	45076-45088	RB	denotes	respectively
T10553	45088-45089	.	denotes	.
T10554	45089-45179	sentence	denotes	Cover slips were immersed in 4% paraformaldehyde and stained for TRAP activity after 2 h.
T10555	45090-45095	NN	denotes	Cover
T10556	45096-45101	NNS	denotes	slips
T10558	45102-45106	VBD	denotes	were
T10557	45107-45115	VBN	denotes	immersed
T10559	45116-45118	IN	denotes	in
T10560	45119-45120	CD	denotes	4
T10561	45120-45121	NN	denotes	%
T10562	45122-45138	NN	denotes	paraformaldehyde
T10563	45139-45142	CC	denotes	and
T10564	45143-45150	VBN	denotes	stained
T10565	45151-45154	IN	denotes	for
T10566	45155-45159	NN	denotes	TRAP
T10567	45160-45168	NN	denotes	activity
T10568	45169-45174	IN	denotes	after
T10569	45175-45176	CD	denotes	2
T10570	45177-45178	NN	denotes	h
T10571	45178-45179	.	denotes	.
T10572	45179-45402	sentence	denotes	Dentin slices were left for 28 h before removing the cells with 1 M ammonium hydroxide and air drying before coating with sputter Au-Pd and examination with scanning electron microscopy (McGill Electron Microscopy Centre).
T10573	45180-45186	NN	denotes	Dentin
T10574	45187-45193	NNS	denotes	slices
T10576	45194-45198	VBD	denotes	were
T10575	45199-45203	VBN	denotes	left
T10577	45204-45207	IN	denotes	for
T10578	45208-45210	CD	denotes	28
T10579	45211-45212	NN	denotes	h
T10580	45213-45219	IN	denotes	before
T10581	45220-45228	VBG	denotes	removing
T10582	45229-45232	DT	denotes	the
T10583	45233-45238	NNS	denotes	cells
T10584	45239-45243	IN	denotes	with
T10585	45244-45245	CD	denotes	1
T10586	45246-45247	NN	denotes	M
T10588	45248-45256	NN	denotes	ammonium
T10587	45257-45266	NN	denotes	hydroxide
T10589	45267-45270	CC	denotes	and
T10590	45271-45274	NN	denotes	air
T10591	45275-45281	VBG	denotes	drying
T10592	45282-45288	IN	denotes	before
T10593	45289-45296	VBG	denotes	coating
T10594	45297-45301	IN	denotes	with
T10595	45302-45309	NN	denotes	sputter
T10597	45310-45312	NN	denotes	Au
T10598	45312-45313	HYPH	denotes	-
T10596	45313-45315	NN	denotes	Pd
T10599	45316-45319	CC	denotes	and
T10600	45320-45331	NN	denotes	examination
T10601	45332-45336	IN	denotes	with
T10602	45337-45345	VBG	denotes	scanning
T10604	45346-45354	NN	denotes	electron
T10603	45355-45365	NN	denotes	microscopy
T10605	45366-45367	-LRB-	denotes	(
T10607	45367-45373	NNP	denotes	McGill
T10608	45374-45382	NNP	denotes	Electron
T10609	45383-45393	NNP	denotes	Microscopy
T10606	45394-45400	NNP	denotes	Centre
T10610	45400-45401	-RRB-	denotes	)
T10611	45401-45402	.	denotes	.
T10612	45402-45505	sentence	denotes	Light microscope images were captured using a Leica DMR microscope equipped with a Retiga 1300 camera.
T10613	45403-45408	NN	denotes	Light
T10614	45409-45419	NN	denotes	microscope
T10615	45420-45426	NNS	denotes	images
T10617	45427-45431	VBD	denotes	were
T10616	45432-45440	VBN	denotes	captured
T10618	45441-45446	VBG	denotes	using
T10619	45447-45448	DT	denotes	a
T10621	45449-45454	NNP	denotes	Leica
T10622	45455-45458	NNP	denotes	DMR
T10620	45459-45469	NN	denotes	microscope
T10623	45470-45478	VBN	denotes	equipped
T10624	45479-45483	IN	denotes	with
T10625	45484-45485	DT	denotes	a
T10627	45486-45492	NNP	denotes	Retiga
T10628	45493-45497	CD	denotes	1300
T10626	45498-45504	NN	denotes	camera
T10629	45504-45505	.	denotes	.
T10630	45505-45645	sentence	denotes	Quantitative analyses were performed using Adobe Photoshop and the data presented as the mean ± SD of two or three independent experiments.
T10631	45506-45518	JJ	denotes	Quantitative
T10632	45519-45527	NNS	denotes	analyses
T10634	45528-45532	VBD	denotes	were
T10633	45533-45542	VBN	denotes	performed
T10635	45543-45548	VBG	denotes	using
T10636	45549-45554	NNP	denotes	Adobe
T10637	45555-45564	NNP	denotes	Photoshop
T10638	45565-45568	CC	denotes	and
T10639	45569-45572	DT	denotes	the
T10640	45573-45577	NNS	denotes	data
T10641	45578-45587	VBN	denotes	presented
T10642	45588-45590	IN	denotes	as
T10643	45591-45594	DT	denotes	the
T10645	45595-45599	NN	denotes	mean
T10646	45600-45601	SYM	denotes	±
T10644	45602-45604	NN	denotes	SD
T10647	45605-45607	IN	denotes	of
T10648	45608-45611	CD	denotes	two
T10650	45612-45614	CC	denotes	or
T10651	45615-45620	CD	denotes	three
T10652	45621-45632	JJ	denotes	independent
T10649	45633-45644	NNS	denotes	experiments
T10653	45644-45645	.	denotes	.
T10654	45645-45707	sentence	denotes	Statistical comparisons were made using the Student's t test.
T10655	45646-45657	JJ	denotes	Statistical
T10656	45658-45669	NNS	denotes	comparisons
T10658	45670-45674	VBD	denotes	were
T10657	45675-45679	VBN	denotes	made
T10659	45680-45685	VBG	denotes	using
T10660	45686-45689	DT	denotes	the
T10661	45690-45697	NNP	denotes	Student
T10663	45697-45699	POS	denotes	's
T10664	45700-45701	NN	denotes	t
T10662	45702-45706	NN	denotes	test
T10665	45706-45707	.	denotes	.
T10693	45709-45720	NN	denotes	Preparation
T10694	45721-45723	IN	denotes	of
T10695	45724-45729	NN	denotes	mouse
T10697	45730-45739	JJ	denotes	embryonic
T10696	45740-45750	NN	denotes	fibroblast
T10698	45751-45754	CC	denotes	and
T10699	45755-45764	NN	denotes	induction
T10700	45765-45767	IN	denotes	of
T10701	45768-45777	NN	denotes	adipocyte
T10702	45778-45793	NN	denotes	differentiation
T10703	45793-45794	.	denotes	.
T10704	45794-45840	sentence	denotes	MEFs were prepared from 14.5-day-old embryos.
T10705	45795-45799	NNS	denotes	MEFs
T10707	45800-45804	VBD	denotes	were
T10706	45805-45813	VBN	denotes	prepared
T10708	45814-45818	IN	denotes	from
T10709	45819-45823	CD	denotes	14.5
T10711	45823-45824	HYPH	denotes	-
T10710	45824-45827	NN	denotes	day
T10713	45827-45828	HYPH	denotes	-
T10712	45828-45831	JJ	denotes	old
T10714	45832-45839	NNS	denotes	embryos
T10715	45839-45840	.	denotes	.
T10716	45840-46015	sentence	denotes	Only early-passage MEFs were used for the experimental studies, and induction of adipocyte differentiation was carried out according to the methods previously described [30].
T10717	45841-45845	RB	denotes	Only
T10719	45846-45851	JJ	denotes	early
T10721	45851-45852	HYPH	denotes	-
T10720	45852-45859	NN	denotes	passage
T10718	45860-45864	NNS	denotes	MEFs
T10723	45865-45869	VBD	denotes	were
T10722	45870-45874	VBN	denotes	used
T10724	45875-45878	IN	denotes	for
T10725	45879-45882	DT	denotes	the
T10727	45883-45895	JJ	denotes	experimental
T10726	45896-45903	NNS	denotes	studies
T10728	45903-45905	,	denotes	,
T10729	45905-45908	CC	denotes	and
T10730	45909-45918	NN	denotes	induction
T10732	45919-45921	IN	denotes	of
T10733	45922-45931	NN	denotes	adipocyte
T10734	45932-45947	NN	denotes	differentiation
T10735	45948-45951	VBD	denotes	was
T10731	45952-45959	VBN	denotes	carried
T10736	45960-45963	RP	denotes	out
T10737	45964-45973	VBG	denotes	according
T10738	45974-45976	IN	denotes	to
T10739	45977-45980	DT	denotes	the
T10740	45981-45988	NNS	denotes	methods
T10741	45989-45999	RB	denotes	previously
T10742	46000-46009	VBN	denotes	described
T10743	46010-46011	-LRB-	denotes	[
T10744	46011-46013	CD	denotes	30
T10745	46013-46014	-RRB-	denotes	]
T10746	46014-46015	.	denotes	.
T10747	46015-46077	sentence	denotes	Cultured cells were stained with Oil Red O as described [29].
T10748	46016-46024	VBN	denotes	Cultured
T10749	46025-46030	NNS	denotes	cells
T10751	46031-46035	VBD	denotes	were
T10750	46036-46043	VBN	denotes	stained
T10752	46044-46048	IN	denotes	with
T10753	46049-46052	NN	denotes	Oil
T10755	46053-46056	NN	denotes	Red
T10754	46057-46058	NN	denotes	O
T10756	46059-46061	IN	denotes	as
T10757	46062-46071	VBN	denotes	described
T10758	46072-46073	-LRB-	denotes	[
T10759	46073-46075	CD	denotes	29
T10760	46075-46076	-RRB-	denotes	]
T10761	46076-46077	.	denotes	.
T10818	46079-46082	NN	denotes	RNA
T10819	46083-46094	NN	denotes	preparation
T10820	46095-46098	CC	denotes	and
T10821	46099-46101	NN	denotes	RT
T10823	46101-46102	HYPH	denotes	-
T10822	46102-46105	NN	denotes	PCR
T10824	46106-46108	TO	denotes	to
T10825	46109-46115	VB	denotes	assess
T10826	46116-46128	NN	denotes	adipogenesis
T10827	46129-46131	IN	denotes	in
T10828	46132-46137	NN	denotes	Sam68
T10830	46137-46138	SYM	denotes	−
T10831	46138-46139	HYPH	denotes	/
T10832	46139-46140	SYM	denotes	−
T10829	46141-46145	NNS	denotes	MEFs
T10833	46145-46146	.	denotes	.
T10834	46146-46288	sentence	denotes	Total cellular RNA was prepared by TRIzol reagent according to the manufacturer's protocol (Invitrogen, Carlsbad, California, United States).
T10835	46147-46152	JJ	denotes	Total
T10837	46153-46161	JJ	denotes	cellular
T10836	46162-46165	NN	denotes	RNA
T10839	46166-46169	VBD	denotes	was
T10838	46170-46178	VBN	denotes	prepared
T10840	46179-46181	IN	denotes	by
T10841	46182-46188	NN	denotes	TRIzol
T10842	46189-46196	NN	denotes	reagent
T10843	46197-46206	VBG	denotes	according
T10844	46207-46209	IN	denotes	to
T10845	46210-46213	DT	denotes	the
T10846	46214-46226	NN	denotes	manufacturer
T10848	46226-46228	POS	denotes	's
T10847	46229-46237	NN	denotes	protocol
T10849	46238-46239	-LRB-	denotes	(
T10850	46239-46249	NNP	denotes	Invitrogen
T10851	46249-46251	,	denotes	,
T10852	46251-46259	NNP	denotes	Carlsbad
T10853	46259-46261	,	denotes	,
T10854	46261-46271	NNP	denotes	California
T10855	46271-46273	,	denotes	,
T10856	46273-46279	NNP	denotes	United
T10857	46280-46286	NNP	denotes	States
T10858	46286-46287	-RRB-	denotes	)
T10859	46287-46288	.	denotes	.
T10860	46288-46370	sentence	denotes	Total RNA (1 μg) was reverse-transcribed, and cDNA samples were subjected to PCR.
T10861	46289-46294	JJ	denotes	Total
T10862	46295-46298	NN	denotes	RNA
T10864	46299-46300	-LRB-	denotes	(
T10866	46300-46301	CD	denotes	1
T10865	46302-46304	NN	denotes	μg
T10867	46304-46305	-RRB-	denotes	)
T10868	46306-46309	VBD	denotes	was
T10869	46310-46317	RB	denotes	reverse
T10870	46317-46318	HYPH	denotes	-
T10863	46318-46329	VBN	denotes	transcribed
T10871	46329-46331	,	denotes	,
T10872	46331-46334	CC	denotes	and
T10873	46335-46339	NN	denotes	cDNA
T10874	46340-46347	NNS	denotes	samples
T10876	46348-46352	VBD	denotes	were
T10875	46353-46362	VBN	denotes	subjected
T10877	46363-46365	IN	denotes	to
T10878	46366-46369	NN	denotes	PCR
T10879	46369-46370	.	denotes	.
T10880	46370-46431	sentence	denotes	RT-PCR was normalized by the transcriptional level of GAPDH.
T10881	46371-46373	NN	denotes	RT
T10883	46373-46374	HYPH	denotes	-
T10882	46374-46377	NN	denotes	PCR
T10885	46378-46381	VBD	denotes	was
T10884	46382-46392	VBN	denotes	normalized
T10886	46393-46395	IN	denotes	by
T10887	46396-46399	DT	denotes	the
T10889	46400-46415	JJ	denotes	transcriptional
T10888	46416-46421	NN	denotes	level
T10890	46422-46424	IN	denotes	of
T10891	46425-46430	NN	denotes	GAPDH
T10892	46430-46431	.	denotes	.
T10893	46431-46832	sentence	denotes	The following 5′ and 3′ primers were used to evaluate adipogenic differentiation: KLF5: 5'-AGA CAA GCT GAG ATG CTG C-3′, 5′-GGC AAA CCT CCA GTC GC-3′; PPARγ: 5′-GTG CGA TCA AAG TAG AAC CTG C-3′, 5′-CCT ATC ATA AAT AAG CTT CAA TCG-3′; β-Actin: 5′-TAG GCG GAC TGT TAC TGA GC-3′, 5′-AGC CTT CAT ACA TCA AGT TGG-3′; and Sam68: 5′- GTG GAG ACC CCA AAT ATG CCC A-3′ and 5′-AAA CTG CTC CTG ACA GAT ATC A-3′.
T10894	46432-46435	DT	denotes	The
T10896	46436-46445	VBG	denotes	following
T10897	46446-46447	CD	denotes	5
T10898	46447-46448	SYM	denotes	′
T10899	46449-46452	CC	denotes	and
T10900	46453-46454	CD	denotes	3
T10901	46454-46455	SYM	denotes	′
T10895	46456-46463	NNS	denotes	primers
T10903	46464-46468	VBD	denotes	were
T10902	46469-46473	VBN	denotes	used
T10904	46474-46476	TO	denotes	to
T10905	46477-46485	VB	denotes	evaluate
T10906	46486-46496	JJ	denotes	adipogenic
T10907	46497-46512	NN	denotes	differentiation
T10908	46512-46514	:	denotes	:
T10909	46514-46518	NN	denotes	KLF5
T10910	46518-46520	:	denotes	:
T10911	46520-46521	CD	denotes	5
T10912	46521-46522	SYM	denotes	'
T10913	46522-46523	HYPH	denotes	-
T10914	46523-46526	NN	denotes	AGA
T10916	46527-46530	NN	denotes	CAA
T10917	46531-46534	NN	denotes	GCT
T10918	46535-46538	NN	denotes	GAG
T10919	46539-46542	NN	denotes	ATG
T10920	46543-46546	NN	denotes	CTG
T10915	46547-46548	NN	denotes	C
T10921	46548-46549	HYPH	denotes	-
T10922	46549-46550	CD	denotes	3
T10923	46550-46551	SYM	denotes	′
T10924	46551-46553	,	denotes	,
T10925	46553-46554	CD	denotes	5
T10926	46554-46555	SYM	denotes	′
T10927	46555-46556	HYPH	denotes	-
T10928	46556-46559	NN	denotes	GGC
T10930	46560-46563	NN	denotes	AAA
T10931	46564-46567	NN	denotes	CCT
T10932	46568-46571	NN	denotes	CCA
T10933	46572-46575	NN	denotes	GTC
T10929	46576-46578	NN	denotes	GC
T10934	46578-46579	HYPH	denotes	-
T10935	46579-46580	CD	denotes	3
T10936	46580-46581	SYM	denotes	′
T10937	46581-46582	:	denotes	;
T10938	46583-46588	NN	denotes	PPARγ
T10939	46588-46590	:	denotes	:
T10940	46590-46591	CD	denotes	5
T10941	46591-46592	SYM	denotes	′
T10942	46592-46593	HYPH	denotes	-
T10943	46593-46596	NN	denotes	GTG
T10945	46597-46600	NN	denotes	CGA
T10946	46601-46604	NN	denotes	TCA
T10947	46605-46608	NN	denotes	AAG
T10948	46609-46612	NN	denotes	TAG
T10949	46613-46616	NN	denotes	AAC
T10950	46617-46620	NN	denotes	CTG
T10944	46621-46622	NN	denotes	C
T10951	46622-46623	HYPH	denotes	-
T10952	46623-46624	CD	denotes	3
T10953	46624-46625	SYM	denotes	′
T10954	46625-46627	,	denotes	,
T10955	46627-46628	CD	denotes	5
T10956	46628-46629	SYM	denotes	′
T10957	46629-46630	HYPH	denotes	-
T10958	46630-46633	NN	denotes	CCT
T10960	46634-46637	NN	denotes	ATC
T10961	46638-46641	NN	denotes	ATA
T10962	46642-46645	NN	denotes	AAT
T10963	46646-46649	NN	denotes	AAG
T10964	46650-46653	NN	denotes	CTT
T10965	46654-46657	NN	denotes	CAA
T10959	46658-46661	NN	denotes	TCG
T10966	46661-46662	HYPH	denotes	-
T10967	46662-46663	CD	denotes	3
T10968	46663-46664	SYM	denotes	′
T10969	46664-46665	:	denotes	;
T10970	46666-46667	NN	denotes	β
T10972	46667-46668	HYPH	denotes	-
T10971	46668-46673	NN	denotes	Actin
T10973	46673-46675	:	denotes	:
T10974	46675-46676	CD	denotes	5
T10975	46676-46677	SYM	denotes	′
T10976	46677-46678	HYPH	denotes	-
T10977	46678-46681	NN	denotes	TAG
T10979	46682-46685	NN	denotes	GCG
T10980	46686-46689	NN	denotes	GAC
T10981	46690-46693	NN	denotes	TGT
T10982	46694-46697	NN	denotes	TAC
T10983	46698-46701	NN	denotes	TGA
T10978	46702-46704	NN	denotes	GC
T10984	46704-46705	HYPH	denotes	-
T10985	46705-46706	CD	denotes	3
T10986	46706-46707	SYM	denotes	′
T10987	46707-46709	,	denotes	,
T10988	46709-46710	CD	denotes	5
T10989	46710-46711	SYM	denotes	′
T10990	46711-46712	HYPH	denotes	-
T10991	46712-46715	NN	denotes	AGC
T10993	46716-46719	NN	denotes	CTT
T10994	46720-46723	NN	denotes	CAT
T10995	46724-46727	NN	denotes	ACA
T10996	46728-46731	NN	denotes	TCA
T10997	46732-46735	NN	denotes	AGT
T10992	46736-46739	NN	denotes	TGG
T10998	46739-46740	HYPH	denotes	-
T10999	46740-46741	CD	denotes	3
T11000	46741-46742	SYM	denotes	′
T11001	46742-46743	:	denotes	;
T11002	46744-46747	CC	denotes	and
T11003	46748-46753	NN	denotes	Sam68
T11004	46753-46755	:	denotes	:
T11005	46755-46756	CD	denotes	5
T11006	46756-46757	SYM	denotes	′
T11007	46757-46758	HYPH	denotes	-
T11008	46759-46762	NN	denotes	GTG
T11010	46763-46766	NN	denotes	GAG
T11011	46767-46770	NN	denotes	ACC
T11012	46771-46774	NN	denotes	CCA
T11013	46775-46778	NN	denotes	AAT
T11014	46779-46782	NN	denotes	ATG
T11015	46783-46786	NN	denotes	CCC
T11009	46787-46788	NN	denotes	A
T11016	46788-46789	HYPH	denotes	-
T11017	46789-46790	CD	denotes	3
T11018	46790-46791	SYM	denotes	′
T11019	46792-46795	CC	denotes	and
T11020	46796-46797	CD	denotes	5
T11021	46797-46798	SYM	denotes	′
T11022	46798-46799	HYPH	denotes	-
T11023	46799-46802	NN	denotes	AAA
T11025	46803-46806	NN	denotes	CTG
T11026	46807-46810	NN	denotes	CTC
T11027	46811-46814	NN	denotes	CTG
T11028	46815-46818	NN	denotes	ACA
T11029	46819-46822	NN	denotes	GAT
T11030	46823-46826	NN	denotes	ATC
T11024	46827-46828	NN	denotes	A
T11031	46828-46829	HYPH	denotes	-
T11032	46829-46830	CD	denotes	3
T11033	46830-46831	SYM	denotes	′
T11034	46831-46832	.	denotes	.
T11076	46834-46839	NN	denotes	Sam68
T11078	46840-46844	RB	denotes	down
T11079	46844-46845	HYPH	denotes	-
T11077	46845-46855	NN	denotes	regulation
T11080	46856-46861	VBG	denotes	using
T11081	46862-46869	NN	denotes	Sam68sh
T11082	46869-46870	.	denotes	.
T11083	46870-46955	sentence	denotes	To generate an shRNA, 64-base duplex DNA nucleotides were purchased from Invitrogen.
T11084	46871-46873	TO	denotes	To
T11085	46874-46882	VB	denotes	generate
T11087	46883-46885	DT	denotes	an
T11088	46886-46891	NN	denotes	shRNA
T11089	46891-46893	,	denotes	,
T11090	46893-46895	CD	denotes	64
T11092	46895-46896	HYPH	denotes	-
T11091	46896-46900	NN	denotes	base
T11094	46901-46907	NN	denotes	duplex
T11095	46908-46911	NN	denotes	DNA
T11093	46912-46923	NNS	denotes	nucleotides
T11096	46924-46928	VBD	denotes	were
T11086	46929-46938	VBN	denotes	purchased
T11097	46939-46943	IN	denotes	from
T11098	46944-46954	NNP	denotes	Invitrogen
T11099	46954-46955	.	denotes	.
T11100	46955-47091	sentence	denotes	This fragment comprised 19 base residues specific to mouse Sam68 (GATCCCC AAGATGACGAGGAGAATTATTCAAGAGA TAATTCTCCTCGTCATCTT TTTTTGGAAA).
T11101	46956-46960	DT	denotes	This
T11102	46961-46969	NN	denotes	fragment
T11103	46970-46979	VBD	denotes	comprised
T11104	46980-46982	CD	denotes	19
T11106	46983-46987	NN	denotes	base
T11105	46988-46996	NNS	denotes	residues
T11107	46997-47005	JJ	denotes	specific
T11108	47006-47008	IN	denotes	to
T11109	47009-47014	NN	denotes	mouse
T11110	47015-47020	NN	denotes	Sam68
T11111	47021-47022	-LRB-	denotes	(
T11113	47022-47029	NN	denotes	GATCCCC
T11114	47030-47058	NN	denotes	AAGATGACGAGGAGAATTATTCAAGAGA
T11115	47059-47078	NN	denotes	TAATTCTCCTCGTCATCTT
T11112	47079-47089	NN	denotes	TTTTTGGAAA
T11116	47089-47090	-RRB-	denotes	)
T11117	47090-47091	.	denotes	.
T11118	47091-47185	sentence	denotes	This fragment was cloned into pSUPER retro (OligoEngine, Seattle, Washington, United States).
T11119	47092-47096	DT	denotes	This
T11120	47097-47105	NN	denotes	fragment
T11122	47106-47109	VBD	denotes	was
T11121	47110-47116	VBN	denotes	cloned
T11123	47117-47121	IN	denotes	into
T11124	47122-47128	NNP	denotes	pSUPER
T11125	47129-47134	NNP	denotes	retro
T11126	47135-47136	-LRB-	denotes	(
T11127	47136-47147	NNP	denotes	OligoEngine
T11128	47147-47149	,	denotes	,
T11129	47149-47156	NNP	denotes	Seattle
T11130	47156-47158	,	denotes	,
T11131	47158-47168	NNP	denotes	Washington
T11132	47168-47170	,	denotes	,
T11133	47170-47176	NNP	denotes	United
T11134	47177-47183	NNP	denotes	States
T11135	47183-47184	-RRB-	denotes	)
T11136	47184-47185	.	denotes	.
T11137	47185-47294	sentence	denotes	Transfections were performed using LipofectAMINE Plus (Invitrogen) with cloned DNA fragment or empty vector.
T11138	47186-47199	NNS	denotes	Transfections
T11140	47200-47204	VBD	denotes	were
T11139	47205-47214	VBN	denotes	performed
T11141	47215-47220	VBG	denotes	using
T11142	47221-47234	NNP	denotes	LipofectAMINE
T11143	47235-47239	NNP	denotes	Plus
T11144	47240-47241	-LRB-	denotes	(
T11145	47241-47251	NNP	denotes	Invitrogen
T11146	47251-47252	-RRB-	denotes	)
T11147	47253-47257	IN	denotes	with
T11148	47258-47264	VBN	denotes	cloned
T11150	47265-47268	NN	denotes	DNA
T11149	47269-47277	NN	denotes	fragment
T11151	47278-47280	CC	denotes	or
T11152	47281-47286	JJ	denotes	empty
T11153	47287-47293	NN	denotes	vector
T11154	47293-47294	.	denotes	.
T11155	47294-47449	sentence	denotes	At 48 h after transfection, populations were selected for 2.5 μg/ml puromycin and the knockdown observed by immunoblotting with anti-Sam68 (AD1) antibody.
T11156	47295-47297	IN	denotes	At
T11158	47298-47300	CD	denotes	48
T11159	47301-47302	NN	denotes	h
T11160	47303-47308	IN	denotes	after
T11161	47309-47321	NN	denotes	transfection
T11162	47321-47323	,	denotes	,
T11163	47323-47334	NNS	denotes	populations
T11164	47335-47339	VBD	denotes	were
T11157	47340-47348	VBN	denotes	selected
T11165	47349-47352	IN	denotes	for
T11166	47353-47356	CD	denotes	2.5
T11167	47357-47359	NN	denotes	μg
T11169	47359-47360	SYM	denotes	/
T11170	47360-47362	NN	denotes	ml
T11168	47363-47372	NN	denotes	puromycin
T11171	47373-47376	CC	denotes	and
T11172	47377-47380	DT	denotes	the
T11173	47381-47390	NN	denotes	knockdown
T11174	47391-47399	VBN	denotes	observed
T11175	47400-47402	IN	denotes	by
T11176	47403-47417	VBG	denotes	immunoblotting
T11177	47418-47422	IN	denotes	with
T11178	47423-47433	JJ	denotes	anti-Sam68
T11180	47434-47435	-LRB-	denotes	(
T11181	47435-47438	NN	denotes	AD1
T11182	47438-47439	-RRB-	denotes	)
T11179	47440-47448	NN	denotes	antibody
T11183	47448-47449	.	denotes	.
T11232	47451-47461	JJ	denotes	Osteogenic
T11234	47462-47477	NN	denotes	differentiation
T11233	47478-47486	NN	denotes	analysis
T11235	47487-47489	IN	denotes	of
T11236	47490-47497	NN	denotes	C3HT101
T11238	47497-47498	HYPH	denotes	/
T11239	47498-47499	CD	denotes	2
T11237	47500-47505	NNS	denotes	cells
T11240	47505-47506	.	denotes	.
T11241	47506-47635	sentence	denotes	For osteogenic differentiation, C3HT101/2 (ATCC) cells were incubated in 48-well tissue culture plates at 1.25 × 104 cells/cm2 .
T11242	47507-47510	IN	denotes	For
T11244	47511-47521	JJ	denotes	osteogenic
T11245	47522-47537	NN	denotes	differentiation
T11246	47537-47539	,	denotes	,
T11247	47539-47546	NN	denotes	C3HT101
T11249	47546-47547	HYPH	denotes	/
T11250	47547-47548	CD	denotes	2
T11251	47549-47550	-LRB-	denotes	(
T11252	47550-47554	NN	denotes	ATCC
T11253	47554-47555	-RRB-	denotes	)
T11248	47556-47561	NNS	denotes	cells
T11254	47562-47566	VBD	denotes	were
T11243	47567-47576	VBN	denotes	incubated
T11255	47577-47579	IN	denotes	in
T11256	47580-47582	CD	denotes	48
T11258	47582-47583	HYPH	denotes	-
T11257	47583-47587	NN	denotes	well
T11260	47588-47594	NN	denotes	tissue
T11261	47595-47602	NN	denotes	culture
T11259	47603-47609	NNS	denotes	plates
T11262	47610-47612	IN	denotes	at
T11263	47613-47617	CD	denotes	1.25
T11265	47618-47619	SYM	denotes	×
T11264	47620-47623	CD	denotes	104
T11266	47624-47629	NNS	denotes	cells
T11267	47629-47630	SYM	denotes	/
T11268	47630-47633	NN	denotes	cm2
T11269	47634-47635	.	denotes	.
T11270	47635-47776	sentence	denotes	After 24-h incubation, the culture media were changed to fresh media containing 300 ng/ml BMP-2 (Sigma, St. Louis, Missouri, United States).
T11271	47636-47641	IN	denotes	After
T11273	47642-47644	CD	denotes	24
T11275	47644-47645	HYPH	denotes	-
T11274	47645-47646	NN	denotes	h
T11276	47647-47657	NN	denotes	incubation
T11277	47657-47659	,	denotes	,
T11278	47659-47662	DT	denotes	the
T11280	47663-47670	NN	denotes	culture
T11279	47671-47676	NNS	denotes	media
T11281	47677-47681	VBD	denotes	were
T11272	47682-47689	VBN	denotes	changed
T11282	47690-47692	IN	denotes	to
T11283	47693-47698	JJ	denotes	fresh
T11284	47699-47704	NNS	denotes	media
T11285	47705-47715	VBG	denotes	containing
T11286	47716-47719	CD	denotes	300
T11287	47720-47722	NN	denotes	ng
T11289	47722-47723	SYM	denotes	/
T11290	47723-47725	NN	denotes	ml
T11288	47726-47729	NN	denotes	BMP
T11291	47729-47730	HYPH	denotes	-
T11292	47730-47731	CD	denotes	2
T11293	47732-47733	-LRB-	denotes	(
T11294	47733-47738	NNP	denotes	Sigma
T11295	47738-47740	,	denotes	,
T11296	47740-47743	NNP	denotes	St.
T11297	47744-47749	NNP	denotes	Louis
T11298	47749-47751	,	denotes	,
T11299	47751-47759	NNP	denotes	Missouri
T11300	47759-47761	,	denotes	,
T11301	47761-47767	NNP	denotes	United
T11302	47768-47774	NNP	denotes	States
T11303	47774-47775	-RRB-	denotes	)
T11304	47775-47776	.	denotes	.
T11305	47776-47947	sentence	denotes	Total cellular RNA was prepared as described above, and osteocalcin, β-actin, and GAPDH gene expression at indicated times after BMP-2 treatment was quantified by RT-PCR.
T11306	47777-47782	JJ	denotes	Total
T11308	47783-47791	JJ	denotes	cellular
T11307	47792-47795	NN	denotes	RNA
T11310	47796-47799	VBD	denotes	was
T11309	47800-47808	VBN	denotes	prepared
T11311	47809-47811	IN	denotes	as
T11312	47812-47821	VBN	denotes	described
T11313	47822-47827	RB	denotes	above
T11314	47827-47829	,	denotes	,
T11315	47829-47832	CC	denotes	and
T11316	47833-47844	NN	denotes	osteocalcin
T11318	47844-47846	,	denotes	,
T11319	47846-47847	NN	denotes	β
T11321	47847-47848	HYPH	denotes	-
T11320	47848-47853	NN	denotes	actin
T11322	47853-47855	,	denotes	,
T11323	47855-47858	CC	denotes	and
T11324	47859-47864	NN	denotes	GAPDH
T11325	47865-47869	NN	denotes	gene
T11317	47870-47880	NN	denotes	expression
T11327	47881-47883	IN	denotes	at
T11328	47884-47893	VBN	denotes	indicated
T11329	47894-47899	NNS	denotes	times
T11330	47900-47905	IN	denotes	after
T11331	47906-47909	NN	denotes	BMP
T11333	47909-47910	HYPH	denotes	-
T11334	47910-47911	CD	denotes	2
T11332	47912-47921	NN	denotes	treatment
T11335	47922-47925	VBD	denotes	was
T11326	47926-47936	VBN	denotes	quantified
T11336	47937-47939	IN	denotes	by
T11337	47940-47942	NN	denotes	RT
T11339	47942-47943	HYPH	denotes	-
T11338	47943-47946	NN	denotes	PCR
T11340	47946-47947	.	denotes	.
R10	T244	T241	compound	Binding,Protein
R100	T341	T342	quantmod	up,75
R1000	T1712	T1713	det	a,substrate
R1001	T1765	T1760	nsubj	we,report
R1002	T1766	T1767	det	the,generation
R1003	T1767	T1760	dobj	generation,report
R1004	T1768	T1767	prep	of,generation
R1005	T1713	T1710	attr	substrate,is
R1006	T1769	T1770	nmod	Sam68,mice
R1007	T1770	T1768	pobj	mice,of
R1008	T1714	T1713	amod	known,substrate
R1009	T1771	T1769	punct	−,Sam68
R101	T342	T345	nummod	75,%
R1010	T1772	T1769	punct	/,Sam68
R1011	T1773	T1769	punct	−,Sam68
R1012	T1774	T1767	cc	and,generation
R1013	T1775	T1767	conj	analysis,generation
R1014	T1776	T1775	prep	of,analysis
R1015	T1777	T1778	poss	their,phenotype
R1016	T1715	T1713	prep	of,substrate
R1017	T1778	T1776	pobj	phenotype,of
R1018	T1779	T1778	amod	skeletal,phenotype
R1019	T1780	T1760	punct	.,report
R102	T343	T342	quantmod	to,75
R1020	T1716	T1717	compound	Src,kinases
R1021	T1782	T1783	poss	Our,data
R1022	T1717	T1715	pobj	kinases,of
R1023	T1783	T1784	nsubj	data,indicate
R1024	T1785	T1786	mark	that,confers
R1025	T1718	T1719	punct	[,39
R1026	T1786	T1784	ccomp	confers,indicate
R1027	T1787	T1788	det	the,absence
R1028	T1719	T1717	parataxis	39,kinases
R1029	T1788	T1786	nsubj	absence,confers
R103	T344	T342	advmod	approximately,75
R1030	T1789	T1788	prep	of,absence
R1031	T1790	T1789	pobj	Sam68,of
R1032	T1720	T1721	punct	–,42
R1033	T1791	T1786	dobj	resistance,confers
R1034	T1792	T1791	prep	to,resistance
R1035	T1793	T1794	npadvmod	age,related
R1036	T1826	T1827	nmod	bone,marrow
R1037	T1794	T1796	amod	related,loss
R1038	T1795	T1794	punct	-,related
R1039	T1796	T1792	pobj	loss,to
R104	T345	T336	npadvmod	%,decreased
R1040	T1797	T1796	compound	bone,loss
R1041	T1798	T1786	prep	in,confers
R1042	T1799	T1798	pobj	mice,in
R1043	T1827	T1828	nmod	marrow,cells
R1044	T1800	T1801	amod	such,have
R1045	T1801	T1786	advcl	have,confers
R1046	T1802	T1801	mark	that,have
R1047	T1828	T1825	pobj	cells,of
R1048	T1803	T1804	amod	old,Sam68
R1049	T1804	T1801	nsubj	Sam68,have
R105	T346	T317	punct	.,preserved
R1050	T1805	T1806	det	a,mass
R1051	T1829	T1828	amod	stromal,cells
R1052	T1806	T1801	dobj	mass,have
R1053	T1807	T1806	amod	higher,mass
R1054	T1808	T1806	compound	bone,mass
R1055	T1830	T1818	prep	by,provide
R1056	T1809	T1806	prep	than,mass
R1057	T1810	T1811	poss	their,littermates
R1058	T1811	T1809	pobj	littermates,than
R1059	T1831	T1830	pcomp	showing,by
R106	T348	T349	prep	In,was
R1060	T1812	T1813	amod	wild,type
R1061	T1813	T1811	compound	type,littermates
R1062	T1814	T1813	punct	-,type
R1063	T1815	T1784	punct	.,indicate
R1064	T1817	T1818	nsubj	We,provide
R1065	T1832	T1833	mark	that,had
R1066	T1819	T1818	dobj	evidence,provide
R1067	T1820	T1821	mark	that,regulates
R1068	T1833	T1831	ccomp	had,showing
R1069	T1821	T1819	acl	regulates,evidence
R107	T350	T348	pobj	fact,In
R1070	T1822	T1821	nsubj	Sam68,regulates
R1071	T1823	T1824	det	the,differentiation
R1072	T1834	T1833	nsubj	cells,had
R1073	T1824	T1821	dobj	differentiation,regulates
R1074	T1825	T1824	prep	of,differentiation
R1075	T1835	T1834	acl	isolated,cells
R1076	T1836	T1835	prep	from,isolated
R1077	T1837	T1838	nmod	Sam68,animals
R1078	T1838	T1836	pobj	animals,from
R1079	T1839	T1837	punct	−,Sam68
R108	T351	T349	punct	", ",was
R1080	T1840	T1837	punct	/,Sam68
R1081	T1841	T1837	punct	−,Sam68
R1082	T1842	T1843	amod	enhanced,activity
R1083	T1843	T1833	dobj	activity,had
R1084	T1844	T1843	amod	osteogenic,activity
R1085	T1845	T1843	cc	and,activity
R1086	T1846	T1847	amod	decreased,activity
R1087	T1847	T1843	conj	activity,activity
R1088	T1848	T1847	amod	adipogenic,activity
R1089	T1849	T1843	prep	than,activity
R109	T352	T353	det	the,volume
R1090	T1850	T1849	pobj	those,than
R1091	T1851	T1850	acl	harvested,those
R1092	T1852	T1851	prep	from,harvested
R1093	T1853	T1854	amod	wild,type
R1094	T1854	T1856	compound	type,littermates
R1095	T1855	T1854	punct	-,type
R1096	T1856	T1852	pobj	littermates,from
R1097	T1857	T1818	punct	.,provide
R1098	T1859	T1860	advmod	Furthermore,impaired
R1099	T1861	T1860	punct	", ",impaired
R11	T245	T237	dobj	Mice,Protects
R110	T353	T349	nsubj	volume,was
R1100	T1862	T1860	nsubjpass	Sam68,impaired
R1101	T1863	T1862	punct	−,Sam68
R1102	T1864	T1862	punct	/,Sam68
R1103	T1865	T1862	punct	−,Sam68
R1104	T1866	T1867	compound	mouse,embryo
R1105	T1867	T1868	compound	embryo,fibroblasts
R1106	T1868	T1862	appos	fibroblasts,Sam68
R1107	T1869	T1868	punct	(,fibroblasts
R1108	T1870	T1868	appos	MEFs,fibroblasts
R1109	T1871	T1862	punct	),Sam68
R111	T354	T353	compound	bone,volume
R1110	T1872	T1860	auxpass	were,impaired
R1111	T1873	T1860	prep	in,impaired
R1112	T1874	T1875	poss	their,capacity
R1113	T1875	T1873	pobj	capacity,in
R1114	T1876	T1877	aux	to,differentiate
R1115	T1877	T1875	acl	differentiate,capacity
R1116	T1878	T1877	prep	into,differentiate
R1117	T1879	T1878	pobj	adipocytes,into
R1118	T1880	T1860	punct	", ",impaired
R1119	T1881	T1860	advcl	consistent,impaired
R112	T355	T353	prep	of,volume
R1120	T1882	T1881	prep	with,consistent
R1121	T1883	T1884	nsubj	Sam68,being
R1122	T1884	T1882	pcomp	being,with
R1123	T1885	T1886	det	a,regulator
R1124	T1886	T1884	attr	regulator,being
R1125	T1887	T1886	prep	of,regulator
R1126	T1888	T1889	nmod	bone,marrow
R1127	T1889	T1890	nmod	marrow,differentiation
R1128	T1890	T1887	pobj	differentiation,of
R1129	T1891	T1890	amod	mesenchymal,differentiation
R113	T356	T357	det	the,mice
R1130	T1892	T1890	compound	cell,differentiation
R1131	T1893	T1860	punct	.,impaired
R1132	T1895	T1896	det	These,results
R1133	T1896	T1897	nsubj	results,characterize
R1134	T1898	T1897	advmod	also,characterize
R1135	T1899	T1900	det	a,model
R1136	T1900	T1897	dobj	model,characterize
R1137	T1901	T1900	amod	new,model
R1138	T1902	T1900	compound	animal,model
R1139	T1903	T1904	aux	to,study
R114	T357	T355	pobj	mice,of
R1140	T1904	T1900	advcl	study,model
R1141	T1905	T1906	compound	bone,metabolism
R1142	T1906	T1904	dobj	metabolism,study
R1143	T1907	T1906	punct	", ",metabolism
R1144	T1908	T1906	conj	regeneration,metabolism
R1145	T1909	T1908	punct	", ",regeneration
R1146	T1910	T1908	cc	and,regeneration
R1147	T1911	T1908	conj	repair,regeneration
R1148	T1912	T1904	prep	during,study
R1149	T1913	T1912	pobj	aging,during
R115	T358	T359	nummod	12,month
R1150	T1914	T1897	punct	.,characterize
R1155	T2061	T2062	nsubjpass	Sam68,Expressed
R1156	T2063	T2062	auxpass	Is,Expressed
R1157	T2064	T2062	prep	in,Expressed
R1158	T2065	T2066	det	the,Skeleton
R1159	T2066	T2064	pobj	Skeleton,in
R116	T359	T361	npadvmod	month,old
R1160	T2067	T2066	amod	Developing,Skeleton
R1161	T2068	T2066	prep	of,Skeleton
R1162	T2069	T2070	amod	Embryonic,Mice
R1163	T2070	T2068	pobj	Mice,of
R1164	T2072	T2073	det	The,mRNA
R1165	T2073	T2075	nsubjpass	mRNA,known
R1166	T2074	T2073	compound	Sam68,mRNA
R1167	T2076	T2075	auxpass	is,known
R1168	T2077	T2078	aux	to,expressed
R1169	T2078	T2075	xcomp	expressed,known
R117	T360	T359	punct	-,month
R1170	T2079	T2078	auxpass	be,expressed
R1171	T2080	T2078	advmod	widely,expressed
R1172	T2081	T2082	punct	[,38
R1173	T2082	T2078	parataxis	38,expressed
R1174	T2083	T2082	punct	],38
R1175	T2084	T2078	punct	", ",expressed
R1176	T2085	T2086	mark	whereas,remains
R1177	T2086	T2078	advcl	remains,expressed
R1178	T2087	T2088	poss	its,pattern
R1179	T2088	T2086	nsubj	pattern,remains
R118	T361	T357	amod	old,mice
R1180	T2089	T2088	prep	of,pattern
R1181	T2090	T2091	compound	protein,expression
R1182	T2091	T2089	pobj	expression,of
R1183	T2092	T2093	advmod	in,vivo
R1184	T2093	T2088	advmod	vivo,pattern
R1185	T2094	T2095	aux	to,defined
R1186	T2095	T2086	xcomp	defined,remains
R1187	T2096	T2095	auxpass	be,defined
R1188	T2097	T2075	punct	.,known
R1189	T2099	T2100	nsubjpass	Sections,immunostained
R119	T362	T361	punct	-,old
R1190	T2101	T2099	prep	of,Sections
R1191	T2102	T2103	npadvmod	paraffin,embedded
R1192	T2103	T2105	amod	embedded,mice
R1193	T2104	T2103	punct	-,embedded
R1194	T2105	T2101	pobj	mice,of
R1195	T2106	T2107	amod	wild,type
R1196	T2107	T2105	nmod	type,mice
R1197	T2108	T2107	punct	-,type
R1198	T2109	T2105	nmod	E14.5,mice
R1199	T2110	T2109	cc	and,E14.5
R12	T246	T237	prep	from,Protects
R120	T363	T357	nmod	Sam68,mice
R1200	T2111	T2109	conj	E16.5,E14.5
R1201	T2112	T2105	amod	embryonic,mice
R1202	T2113	T2100	auxpass	were,immunostained
R1203	T2114	T2100	prep	with,immunostained
R1204	T2115	T2116	det	the,antibody
R1205	T2116	T2114	pobj	antibody,with
R1206	T2117	T2118	advmod	well,characterized
R1207	T2118	T2116	amod	characterized,antibody
R1208	T2119	T2118	punct	-,characterized
R1209	T2120	T2116	nmod	AD1,antibody
R121	T364	T363	punct	−,Sam68
R1210	T2121	T2116	amod	anti-Sam68,antibody
R1211	T2122	T2116	punct	", ",antibody
R1212	T2123	T2116	acl	raised,antibody
R1213	T2124	T2123	prep	in,raised
R1214	T2125	T2124	pobj	rabbits,in
R1215	T2126	T2116	prep	against,antibody
R1216	T2127	T2128	det	an,peptide
R1217	T2128	T2126	pobj	peptide,against
R1218	T2129	T2128	amod	immunizing,peptide
R1219	T2130	T2131	dep	that,corresponds
R122	T365	T363	punct	/,Sam68
R1220	T2131	T2128	relcl	corresponds,peptide
R1221	T2132	T2131	prep	to,corresponds
R1222	T2133	T2134	nmod	amino,330
R1223	T2134	T2132	pobj	330,to
R1224	T2135	T2134	nmod	acids,330
R1225	T2136	T2134	prep	to,330
R1226	T2137	T2136	pobj	348,to
R1227	T2138	T2134	prep	of,330
R1228	T2139	T2140	det	the,protein
R1229	T2140	T2138	pobj	protein,of
R123	T366	T363	punct	−,Sam68
R1230	T2141	T2140	compound	mouse,protein
R1231	T2142	T2140	compound	Sam68,protein
R1232	T2143	T2144	punct	[,46
R1233	T2144	T2100	parataxis	46,immunostained
R1234	T2145	T2144	punct	],46
R1235	T2146	T2100	punct	.,immunostained
R1236	T2148	T2149	compound	Sam68,immunoreactivity
R1237	T2149	T2150	nsubjpass	immunoreactivity,observed
R1238	T2151	T2150	auxpass	was,observed
R1239	T2152	T2150	prep	in,observed
R124	T367	T368	advmod	virtually,indistinguishable
R1240	T2153	T2154	det	the,skeleton
R1241	T2154	T2152	pobj	skeleton,in
R1242	T2155	T2154	cc	and,skeleton
R1243	T2156	T2157	amod	soft,tissues
R1244	T2157	T2154	conj	tissues,skeleton
R1245	T2158	T2154	prep	of,skeleton
R1246	T2159	T2160	amod	developing,mice
R1247	T2160	T2158	pobj	mice,of
R1248	T2161	T2162	amod	wild,type
R1249	T2162	T2160	compound	type,mice
R125	T368	T349	acomp	indistinguishable,was
R1250	T2163	T2162	punct	-,type
R1251	T2164	T2150	prep	at,observed
R1252	T2165	T2164	pobj	E14.5,at
R1253	T2166	T2165	cc	and,E14.5
R1254	T2167	T2165	conj	E16.5,E14.5
R1255	T2168	T2169	punct	(,Figure
R1256	T2169	T2150	parataxis	Figure,observed
R1257	T2170	T2169	nummod	1,Figure
R1258	T2171	T2169	punct	),Figure
R1259	T2172	T2150	punct	.,observed
R126	T369	T368	prep	from,indistinguishable
R1260	T2174	T2175	amod	Intense,staining
R1261	T2175	T2176	nsubjpass	staining,seen
R1262	T2177	T2176	auxpass	was,seen
R1263	T2178	T2176	prep	in,seen
R1264	T2179	T2180	det	the,nucleus
R1265	T2180	T2178	pobj	nucleus,in
R1266	T2181	T2180	prep	of,nucleus
R1267	T2182	T2181	pobj	cells,of
R1268	T2183	T2176	prep	in,seen
R1269	T2184	T2185	det	the,brain
R127	T370	T369	pobj	that,from
R1270	T2185	T2183	pobj	brain,in
R1271	T2186	T2185	amod	developing,brain
R1272	T2187	T2185	punct	", ",brain
R1273	T2188	T2185	conj	heart,brain
R1274	T2189	T2188	punct	", ",heart
R1275	T2190	T2188	cc	and,heart
R1276	T2191	T2192	amod	small,intestine
R1277	T2192	T2188	conj	intestine,heart
R1278	T2193	T2194	punct	(,1B
R1279	T2194	T2185	parataxis	1B,brain
R128	T371	T370	prep	of,that
R1280	T2195	T2194	compound	Figure,1B
R1281	T2196	T2194	punct	),1B
R1282	T2197	T2183	punct	", ",in
R1283	T2198	T2199	advmod	as,as
R1284	T2199	T2183	cc	as,in
R1285	T2200	T2199	advmod	well,as
R1286	T2201	T2183	conj	in,in
R1287	T2202	T2201	pobj	chondrocytes,in
R1288	T2203	T2202	prep	in,chondrocytes
R1289	T2204	T2205	det	the,septum
R129	T372	T373	nummod	4,month
R1290	T2205	T2203	pobj	septum,in
R1291	T2206	T2205	amod	nasal,septum
R1292	T2207	T2205	cc	and,septum
R1293	T2208	T2209	det	the,tissue
R1294	T2209	T2205	conj	tissue,septum
R1295	T2210	T2209	amod	glandular,tissue
R1296	T2211	T2209	amod	adjacent,tissue
R1297	T2212	T2211	prep	to,adjacent
R1298	T2213	T2214	det	the,cartilage
R1299	T2214	T2212	pobj	cartilage,to
R13	T247	T248	npadvmod	Age,Related
R130	T373	T375	npadvmod	month,old
R1300	T2215	T2214	amod	nasal,cartilage
R1301	T2216	T2217	punct	(,1C
R1302	T2217	T2202	parataxis	1C,chondrocytes
R1303	T2218	T2217	compound	Figure,1C
R1304	T2219	T2217	punct	", ",1C
R1305	T2220	T2221	compound	panels,A
R1306	T2221	T2217	appos	A,1C
R1307	T2222	T2223	punct	–,D
R1308	T2223	T2221	prep	D,A
R1309	T2224	T2217	punct	),1C
R131	T374	T373	punct	-,month
R1310	T2225	T2201	punct	", ",in
R1311	T2226	T2201	prep	in,in
R1312	T2227	T2228	det	the,vertebra
R1313	T2228	T2226	pobj	vertebra,in
R1314	T2229	T2228	cc	and,vertebra
R1315	T2230	T2231	amod	intervertebral,discs
R1316	T2231	T2228	conj	discs,vertebra
R1317	T2232	T2233	punct	(,E
R1318	T2233	T2228	parataxis	E,vertebra
R1319	T2234	T2233	compound	panels,E
R132	T375	T377	amod	old,mice
R1320	T2235	T2236	punct	–,H
R1321	T2236	T2233	prep	H,E
R1322	T2237	T2233	punct	),E
R1323	T2238	T2226	cc	and,in
R1324	T2239	T2226	conj	in,in
R1325	T2240	T2241	det	the,epiphysis
R1326	T2241	T2239	pobj	epiphysis,in
R1327	T2242	T2243	punct	(,I
R1328	T2243	T2241	parataxis	I,epiphysis
R1329	T2244	T2243	compound	panels,I
R133	T376	T375	punct	-,old
R1330	T2245	T2246	punct	–,K
R1331	T2246	T2243	prep	K,I
R1332	T2247	T2243	punct	),I
R1333	T2248	T2241	cc	and,epiphysis
R1334	T2249	T2241	conj	metaphysis,epiphysis
R1335	T2250	T2251	punct	(,L
R1336	T2251	T2249	parataxis	L,metaphysis
R1337	T2252	T2251	compound	panel,L
R1338	T2253	T2251	punct	),L
R1339	T2254	T2241	prep	of,epiphysis
R134	T377	T371	pobj	mice,of
R1340	T2255	T2256	amod	long,bones
R1341	T2256	T2254	pobj	bones,of
R1342	T2257	T2176	punct	.,seen
R1343	T2259	T2260	amod	Nuclear,staining
R1344	T2260	T2261	nsubjpass	staining,observed
R1345	T2262	T2261	auxpass	was,observed
R1346	T2263	T2261	prep	in,observed
R1347	T2264	T2265	amod	proliferating,chondrocytes
R1348	T2265	T2263	pobj	chondrocytes,in
R1349	T2266	T2265	cc	and,chondrocytes
R135	T378	T379	amod	wild,type
R1350	T2267	T2268	amod	hypertrophic,chondrocytes
R1351	T2268	T2265	conj	chondrocytes,chondrocytes
R1352	T2269	T2270	punct	(,1C
R1353	T2270	T2268	parataxis	1C,chondrocytes
R1354	T2271	T2270	compound	Figure,1C
R1355	T2272	T2270	punct	", ",1C
R1356	T2273	T2274	compound	panel,H
R1357	T2274	T2270	appos	H,1C
R1358	T2275	T2270	punct	),1C
R1359	T2276	T2263	punct	", ",in
R136	T379	T377	nmod	type,mice
R1360	T2277	T2263	conj	in,in
R1361	T2278	T2277	pobj	osteoblasts,in
R1362	T2279	T2280	punct	(,L
R1363	T2280	T2278	parataxis	L,osteoblasts
R1364	T2281	T2280	compound	panel,L
R1365	T2282	T2280	punct	;,L
R1366	T2283	T2284	compound	Dataset,S1
R1367	T2284	T2280	appos	S1,L
R1368	T2285	T2280	punct	),L
R1369	T2286	T2277	punct	", ",in
R137	T380	T379	punct	-,type
R1370	T2287	T2277	cc	and,in
R1371	T2288	T2277	conj	in,in
R1372	T2289	T2288	pobj	osteoclasts,in
R1373	T2290	T2291	punct	(,S1
R1374	T2291	T2289	parataxis	S1,osteoclasts
R1375	T2292	T2291	compound	Dataset,S1
R1376	T2293	T2291	punct	),S1
R1377	T2294	T2261	punct	.,observed
R1378	T2296	T2297	det	No,staining
R1379	T2297	T2298	nsubjpass	staining,observed
R138	T381	T379	cc	or,type
R1380	T2299	T2298	auxpass	was,observed
R1381	T2300	T2298	prep	with,observed
R1382	T2301	T2302	amod	preimmune,serum
R1383	T2302	T2300	pobj	serum,with
R1384	T2303	T2300	cc	or,with
R1385	T2304	T2305	advmod	when,preadsorbed
R1386	T2305	T2300	conj	preadsorbed,with
R1387	T2306	T2307	det	the,antiserum
R1388	T2307	T2305	nsubjpass	antiserum,preadsorbed
R1389	T2308	T2305	auxpass	was,preadsorbed
R139	T382	T379	conj	Sam68,type
R1390	T2309	T2305	prep	with,preadsorbed
R1391	T2310	T2311	det	the,peptide
R1392	T2311	T2309	pobj	peptide,with
R1393	T2312	T2311	amod	immunizing,peptide
R1394	T2313	T2314	punct	(,1C
R1395	T2314	T2305	parataxis	1C,preadsorbed
R1396	T2315	T2314	compound	Figure,1C
R1397	T2316	T2314	punct	", ",1C
R1398	T2317	T2318	compound	panels,B
R1399	T2318	T2314	appos	B,1C
R14	T248	T250	amod	Related,Loss
R140	T383	T382	punct	−,Sam68
R1400	T2319	T2318	punct	", ",B
R1401	T2320	T2318	conj	F,B
R1402	T2321	T2320	punct	", ",F
R1403	T2322	T2320	cc	and,F
R1404	T2323	T2320	conj	J,F
R1405	T2324	T2314	punct	),1C
R1406	T2325	T2298	punct	.,observed
R1407	T2327	T2328	advcl	Taken,demonstrate
R1408	T2329	T2327	advmod	together,Taken
R1409	T2330	T2328	punct	", ",demonstrate
R141	T384	T382	punct	/,Sam68
R1410	T2331	T2332	det	these,data
R1411	T2332	T2328	nsubj	data,demonstrate
R1412	T2333	T2334	mark	that,expressed
R1413	T2334	T2328	ccomp	expressed,demonstrate
R1414	T2335	T2336	compound	Sam68,protein
R1415	T2336	T2334	nsubjpass	protein,expressed
R1416	T2337	T2334	auxpass	is,expressed
R1417	T2338	T2334	advmod	selectively,expressed
R1418	T2339	T2334	prep	in,expressed
R1419	T2340	T2341	det	the,embryo
R142	T385	T382	punct	−,Sam68
R1420	T2341	T2339	pobj	embryo,in
R1421	T2342	T2341	amod	developing,embryo
R1422	T2343	T2341	compound	mouse,embryo
R1423	T2344	T2334	punct	", ",expressed
R1424	T2345	T2334	prep	with,expressed
R1425	T2346	T2347	advmod	particularly,elevated
R1426	T2347	T2348	amod	elevated,expression
R1427	T2348	T2345	pobj	expression,with
R1428	T2349	T2348	prep	in,expression
R1429	T2350	T2349	pobj	cartilage,in
R143	T386	T349	punct	.,was
R1430	T2351	T2350	cc	and,cartilage
R1431	T2352	T2350	conj	bone,cartilage
R1432	T2353	T2328	punct	.,demonstrate
R1433	T2520	T2521	nsubj	Sam68,is
R1434	T2522	T2521	neg	Not,is
R1435	T2523	T2521	acomp	Essential,is
R1436	T2524	T2523	prep	for,Essential
R1437	T2525	T2526	compound	Mouse,Development
R1438	T2526	T2524	pobj	Development,for
R1439	T2528	T2529	aux	To,define
R144	T388	T389	nmod	Sam68,cells
R1440	T2529	T2530	advcl	define,generated
R1441	T2531	T2532	det	the,role
R1442	T2532	T2529	dobj	role,define
R1443	T2533	T2532	amod	physiologic,role
R1444	T2534	T2532	prep	of,role
R1445	T2535	T2534	pobj	Sam68,of
R1446	T2536	T2530	punct	", ",generated
R1447	T2537	T2530	nsubj	we,generated
R1448	T2538	T2539	npadvmod	Sam68,deficient
R1449	T2539	T2541	amod	deficient,mice
R145	T389	T396	nsubj	cells,had
R1450	T2540	T2539	punct	-,deficient
R1451	T2541	T2530	dobj	mice,generated
R1452	T2542	T2530	prep	by,generated
R1453	T2543	T2544	amod	targeted,disruption
R1454	T2544	T2542	pobj	disruption,by
R1455	T2545	T2544	prep	of,disruption
R1456	T2546	T2547	nmod	exons,4
R1457	T2547	T2545	pobj	4,of
R1458	T2548	T2547	cc	and,4
R1459	T2549	T2547	conj	5,4
R146	T390	T388	punct	−,Sam68
R1460	T2550	T2547	prep	of,4
R1461	T2551	T2552	det	the,gene
R1462	T2552	T2550	pobj	gene,of
R1463	T2553	T2552	compound	sam68,gene
R1464	T2554	T2547	punct	", ",4
R1465	T2555	T2556	dep	which,encode
R1466	T2556	T2547	relcl	encode,4
R1467	T2557	T2558	det	the,region
R1468	T2558	T2556	dobj	region,encode
R1469	T2559	T2558	amod	functional,region
R147	T391	T388	punct	/,Sam68
R1470	T2560	T2558	prep	of,region
R1471	T2561	T2562	det	the,domain
R1472	T2562	T2560	pobj	domain,of
R1473	T2563	T2564	compound	KH,type
R1474	T2564	T2562	compound	type,domain
R1475	T2565	T2564	punct	-,type
R1476	T2566	T2567	compound	RNA,binding
R1477	T2567	T2562	compound	binding,domain
R1478	T2568	T2569	punct	(,2A
R1479	T2569	T2530	parataxis	2A,generated
R148	T392	T388	punct	−,Sam68
R1480	T2570	T2569	compound	Figure,2A
R1481	T2571	T2569	punct	),2A
R1482	T2572	T2530	punct	.,generated
R1483	T2574	T2575	det	The,integrity
R1484	T2575	T2576	nsubjpass	integrity,verified
R1485	T2577	T2575	prep	of,integrity
R1486	T2578	T2579	det	the,allele
R1487	T2579	T2577	pobj	allele,of
R1488	T2580	T2579	amod	targeted,allele
R1489	T2581	T2576	auxpass	was,verified
R149	T393	T394	nmod	bone,marrow
R1490	T2582	T2576	prep	by,verified
R1491	T2583	T2584	compound	Southern,blot
R1492	T2584	T2585	compound	blot,analysis
R1493	T2585	T2582	pobj	analysis,by
R1494	T2586	T2587	punct	(,2B
R1495	T2587	T2585	parataxis	2B,analysis
R1496	T2588	T2587	compound	Figure,2B
R1497	T2589	T2587	punct	),2B
R1498	T2590	T2582	cc	and,by
R1499	T2591	T2582	conj	by,by
R15	T249	T248	punct	-,Related
R150	T394	T389	nmod	marrow,cells
R1500	T2592	T2591	pobj	PCR,by
R1501	T2593	T2592	prep	of,PCR
R1502	T2594	T2595	amod	genomic,DNA
R1503	T2595	T2593	pobj	DNA,of
R1504	T2596	T2597	punct	(,data
R1505	T2597	T2592	meta	data,PCR
R1506	T2598	T2597	amod	unpublished,data
R1507	T2599	T2597	punct	),data
R1508	T2600	T2576	punct	.,verified
R1509	T2602	T2603	compound	Sam68,transcripts
R151	T395	T389	amod	stromal,cells
R1510	T2603	T2604	nsubj	transcripts,were
R1511	T2605	T2603	acl	encoded,transcripts
R1512	T2606	T2605	agent	by,encoded
R1513	T2607	T2608	nmod	exons,1
R1514	T2608	T2606	pobj	1,by
R1515	T2609	T2608	prep	to,1
R1516	T2610	T2609	pobj	5,to
R1517	T2611	T2604	acomp	absent,were
R1518	T2612	T2613	mark	as,evidenced
R1519	T2613	T2604	advcl	evidenced,were
R152	T397	T398	det	a,advantage
R1520	T2614	T2613	prep	by,evidenced
R1521	T2615	T2616	compound	RT,PCR
R1522	T2616	T2614	pobj	PCR,by
R1523	T2617	T2616	punct	-,PCR
R1524	T2618	T2619	punct	(,S2
R1525	T2619	T2613	parataxis	S2,evidenced
R1526	T2620	T2619	compound	Dataset,S2
R1527	T2621	T2619	punct	),S2
R1528	T2622	T2604	punct	", ",were
R1529	T2623	T2604	cc	and,were
R153	T398	T396	dobj	advantage,had
R1530	T2624	T2625	nmod	Sam68,mice
R1531	T2625	T2629	nsubj	mice,were
R1532	T2626	T2624	punct	−,Sam68
R1533	T2627	T2624	punct	/,Sam68
R1534	T2628	T2624	punct	−,Sam68
R1535	T2629	T2604	conj	were,were
R1536	T2630	T2629	acomp	devoid,were
R1537	T2631	T2630	prep	of,devoid
R1538	T2632	T2633	compound	Sam68,protein
R1539	T2633	T2631	pobj	protein,of
R154	T399	T398	compound	differentiation,advantage
R1540	T2634	T2629	punct	", ",were
R1541	T2635	T2636	mark	as,visualized
R1542	T2636	T2629	advcl	visualized,were
R1543	T2637	T2636	prep	by,visualized
R1544	T2638	T2637	pcomp	immunoblotting,by
R1545	T2639	T2638	prep	with,immunoblotting
R1546	T2640	T2641	amod	anti-Sam68,AD1
R1547	T2641	T2642	nmod	AD1,antibodies
R1548	T2642	T2639	pobj	antibodies,with
R1549	T2643	T2641	cc	and,AD1
R155	T400	T398	prep	for,advantage
R1550	T2644	T2641	conj	SC,AD1
R1551	T2645	T2644	punct	-,SC
R1552	T2646	T2644	nummod	333,SC
R1553	T2647	T2642	cc	or,antibodies
R1554	T2648	T2649	nmod	control,serum
R1555	T2649	T2642	conj	serum,antibodies
R1556	T2650	T2649	amod	normal,serum
R1557	T2651	T2649	compound	rabbit,serum
R1558	T2652	T2649	cc	or,serum
R1559	T2653	T2654	amod	anti-Sam68,AD1
R156	T401	T402	det	the,pathway
R1560	T2654	T2649	conj	AD1,serum
R1561	T2655	T2654	acl	preabsorbed,AD1
R1562	T2656	T2655	prep	with,preabsorbed
R1563	T2657	T2656	pobj	peptide,with
R1564	T2658	T2659	punct	(,2C
R1565	T2659	T2629	parataxis	2C,were
R1566	T2660	T2659	compound	Figure,2C
R1567	T2661	T2659	punct	),2C
R1568	T2662	T2629	punct	.,were
R1569	T2664	T2665	nsubj	SC,is
R157	T402	T400	pobj	pathway,for
R1570	T2666	T2664	punct	-,SC
R1571	T2667	T2664	nummod	333,SC
R1572	T2668	T2669	det	a,antibody
R1573	T2669	T2665	attr	antibody,is
R1574	T2670	T2669	nmod	rabbit,antibody
R1575	T2671	T2669	amod	anti-Sam68,antibody
R1576	T2672	T2673	dep	that,raised
R1577	T2673	T2669	relcl	raised,antibody
R1578	T2674	T2673	auxpass	was,raised
R1579	T2675	T2673	prep	against,raised
R158	T403	T402	amod	osteogenic,pathway
R1580	T2676	T2677	det	the,acids
R1581	T2677	T2675	pobj	acids,against
R1582	T2678	T2679	npadvmod	C,terminal
R1583	T2679	T2677	amod	terminal,acids
R1584	T2680	T2679	punct	-,terminal
R1585	T2681	T2677	nummod	20,acids
R1586	T2682	T2677	compound	amino,acids
R1587	T2683	T2677	prep	of,acids
R1588	T2684	T2683	pobj	Sam68,of
R1589	T2685	T2686	punct	[,47
R159	T404	T396	punct	.,had
R1590	T2686	T2665	parataxis	47,is
R1591	T2687	T2686	punct	],47
R1592	T2688	T2665	punct	.,is
R1593	T2690	T2691	det	These,data
R1594	T2691	T2692	nsubj	data,confirmed
R1595	T2693	T2694	det	the,generation
R1596	T2694	T2692	dobj	generation,confirmed
R1597	T2695	T2694	prep	of,generation
R1598	T2696	T2697	det	a,mouse
R1599	T2697	T2695	pobj	mouse,of
R16	T250	T246	pobj	Loss,from
R160	T406	T407	advmod	Moreover,resulted
R1600	T2698	T2697	amod	deficient,mouse
R1601	T2699	T2698	prep	in,deficient
R1602	T2700	T2699	pobj	Sam68,in
R1603	T2701	T2692	punct	.,confirmed
R1604	T2703	T2704	det	The,genotypes
R1605	T2704	T2705	nsubj	genotypes,exhibited
R1606	T2706	T2704	prep	of,genotypes
R1607	T2707	T2706	pobj	offspring,of
R1608	T2708	T2707	prep	from,offspring
R1609	T2709	T2710	compound	heterozygote,intercrosses
R161	T408	T407	punct	", ",resulted
R1610	T2710	T2708	pobj	intercrosses,from
R1611	T2711	T2712	det	a,segregation
R1612	T2712	T2705	dobj	segregation,exhibited
R1613	T2713	T2712	amod	Mendelian,segregation
R1614	T2714	T2705	prep	at,exhibited
R1615	T2715	T2714	pobj	E18.5,at
R1616	T2716	T2717	punct	(,Table
R1617	T2717	T2705	parataxis	Table,exhibited
R1618	T2718	T2717	nummod	1,Table
R1619	T2719	T2717	punct	),Table
R162	T409	T410	det	the,knockdown
R1620	T2720	T2705	punct	.,exhibited
R1621	T2722	T2723	prep	Despite,died
R1622	T2724	T2725	det	the,lack
R1623	T2725	T2722	pobj	lack,Despite
R1624	T2726	T2725	prep	of,lack
R1625	T2727	T2728	amod	visible,deformity
R1626	T2728	T2726	pobj	deformity,of
R1627	T2729	T2723	punct	", ",died
R1628	T2730	T2723	nsubj	many,died
R1629	T2731	T2730	prep	of,many
R163	T410	T407	nsubj	knockdown,resulted
R1630	T2732	T2733	det	the,pups
R1631	T2733	T2731	pobj	pups,of
R1632	T2734	T2733	nmod	Sam68,pups
R1633	T2735	T2734	punct	−,Sam68
R1634	T2736	T2734	punct	/,Sam68
R1635	T2737	T2734	punct	−,Sam68
R1636	T2738	T2723	prep	at,died
R1637	T2739	T2738	pobj	birth,at
R1638	T2740	T2723	prep	of,died
R1639	T2741	T2742	amod	unknown,causes
R164	T411	T410	prep	of,knockdown
R1640	T2742	T2740	pobj	causes,of
R1641	T2743	T2723	punct	.,died
R1642	T2745	T2746	nmod	Sam68,mice
R1643	T2746	T2750	nsubj	mice,were
R1644	T2747	T2745	punct	+,Sam68
R1645	T2748	T2745	punct	/,Sam68
R1646	T2749	T2745	punct	-,Sam68
R1647	T2751	T2752	advmod	phenotypically,normal
R1648	T2752	T2750	acomp	normal,were
R1649	T2753	T2750	cc	and,were
R165	T412	T411	pobj	Sam68,of
R1650	T2754	T2755	nmod	Sam68,pups
R1651	T2755	T2759	nsubj	pups,lived
R1652	T2756	T2754	punct	−,Sam68
R1653	T2757	T2754	punct	/,Sam68
R1654	T2758	T2754	punct	−,Sam68
R1655	T2759	T2750	conj	lived,were
R1656	T2760	T2761	dep	that,survived
R1657	T2761	T2755	relcl	survived,pups
R1658	T2762	T2763	det	the,period
R1659	T2763	T2761	dobj	period,survived
R166	T413	T410	acl	using,knockdown
R1660	T2764	T2763	amod	perinatal,period
R1661	T2765	T2759	advmod	invariably,lived
R1662	T2766	T2759	prep	to,lived
R1663	T2767	T2768	amod	old,age
R1664	T2768	T2766	pobj	age,to
R1665	T2769	T2759	punct	.,lived
R1666	T2771	T2772	prep	Despite,develop
R1667	T2773	T2771	pobj	evidence,Despite
R1668	T2774	T2775	mark	that,expressed
R1669	T2775	T2773	acl	expressed,evidence
R167	T414	T415	amod	short,RNA
R1670	T2776	T2777	compound	Sam68,mRNA
R1671	T2777	T2775	nsubjpass	mRNA,expressed
R1672	T2778	T2775	auxpass	is,expressed
R1673	T2779	T2775	advmod	widely,expressed
R1674	T2780	T2775	cc	and,expressed
R1675	T2781	T2775	conj	phosphorylated,expressed
R1676	T2782	T2781	prep	in,phosphorylated
R1677	T2783	T2782	pobj	mitosis,in
R1678	T2784	T2785	punct	[,42
R1679	T2785	T2775	parataxis	42,expressed
R168	T415	T413	dobj	RNA,using
R1680	T2786	T2785	nummod	41,42
R1681	T2787	T2785	punct	",",42
R1682	T2788	T2785	punct	],42
R1683	T2789	T2772	punct	", ",develop
R1684	T2790	T2791	det	the,mice
R1685	T2791	T2772	nsubj	mice,develop
R1686	T2792	T2791	nmod	Sam68,mice
R1687	T2793	T2792	punct	−,Sam68
R1688	T2794	T2792	punct	/,Sam68
R1689	T2795	T2792	punct	−,Sam68
R169	T416	T415	compound	hairpin,RNA
R1690	T2796	T2772	aux	did,develop
R1691	T2797	T2772	neg	not,develop
R1692	T2798	T2772	dobj	tumors,develop
R1693	T2799	T2772	cc	and,develop
R1694	T2800	T2772	conj	showed,develop
R1695	T2801	T2802	det	no,illnesses
R1696	T2802	T2800	dobj	illnesses,showed
R1697	T2803	T2802	amod	immunologic,illnesses
R1698	T2804	T2803	cc	or,immunologic
R1699	T2805	T2806	amod	other,major
R17	T251	T250	compound	Bone,Loss
R170	T417	T413	prep	in,using
R1700	T2806	T2803	conj	major,immunologic
R1701	T2807	T2772	punct	.,develop
R1702	T2809	T2810	nmod	Sam68,mice
R1703	T2810	T2814	nsubj	mice,have
R1704	T2811	T2809	punct	−,Sam68
R1705	T2812	T2809	punct	/,Sam68
R1706	T2813	T2809	punct	−,Sam68
R1707	T2815	T2814	aux	did,have
R1708	T2816	T2814	punct	", ",have
R1709	T2817	T2814	advmod	however,have
R171	T418	T419	det	the,cells
R1710	T2818	T2814	punct	", ",have
R1711	T2819	T2814	dobj	difficulty,have
R1712	T2820	T2819	acl	breeding,difficulty
R1713	T2821	T2814	prep	due,have
R1714	T2822	T2821	pcomp	to,due
R1715	T2823	T2824	amod	male,infertility
R1716	T2824	T2821	pobj	infertility,due
R1717	T2825	T2814	cc	and,have
R1718	T2826	T2827	det	the,females
R1719	T2827	T2828	nsubj	females,provided
R172	T419	T417	pobj	cells,in
R1720	T2828	T2814	conj	provided,have
R1721	T2829	T2828	advmod	rarely,provided
R1722	T2830	T2831	amod	adequate,care
R1723	T2831	T2828	dobj	care,provided
R1724	T2832	T2828	dative	to,provided
R1725	T2833	T2834	poss	their,young
R1726	T2834	T2832	pobj	young,to
R1727	T2835	T2828	punct	.,provided
R173	T420	T419	amod	embryonic,cells
R1732	T3035	T3036	compound	Sam68,Deficiency
R1733	T3036	T3037	nsubj	Deficiency,Protects
R1734	T3038	T3037	dobj	Mice,Protects
R1735	T3039	T3037	prep	from,Protects
R1736	T3040	T3041	npadvmod	Age,Related
R1737	T3041	T3043	amod	Related,Loss
R1738	T3042	T3041	punct	-,Related
R1739	T3043	T3039	pobj	Loss,from
R174	T421	T419	amod	mesenchymal,cells
R1740	T3044	T3043	compound	Bone,Loss
R1741	T3046	T3047	nmod	Src,animals
R1742	T3047	T3049	nsubjpass	animals,known
R1743	T3048	T3047	amod	null,animals
R1744	T3050	T3049	auxpass	are,known
R1745	T3051	T3052	aux	to,have
R1746	T3052	T3049	xcomp	have,known
R1747	T3053	T3054	compound	bone,metabolism
R1748	T3054	T3055	compound	metabolism,defects
R1749	T3055	T3052	dobj	defects,have
R175	T422	T419	amod	multipotential,cells
R1750	T3056	T3057	punct	[,48
R1751	T3057	T3049	parataxis	48,known
R1752	T3058	T3057	punct	],48
R1753	T3059	T3049	punct	", ",known
R1754	T3060	T3049	cc	and,known
R1755	T3061	T3062	mark	since,is
R1756	T3062	T3064	advcl	is,decided
R1757	T3063	T3062	nsubj	Sam68,is
R1758	T3064	T3049	conj	decided,known
R1759	T3065	T3066	det	a,substrate
R176	T423	T419	nmod	progenitor,cells
R1760	T3066	T3062	attr	substrate,is
R1761	T3067	T3066	prep	of,substrate
R1762	T3068	T3067	pobj	Src,of
R1763	T3069	T3064	punct	", ",decided
R1764	T3070	T3064	nsubj	we,decided
R1765	T3071	T3072	aux	to,analyze
R1766	T3072	T3064	xcomp	analyze,decided
R1767	T3073	T3074	det	the,mice
R1768	T3074	T3072	dobj	mice,analyze
R1769	T3075	T3074	compound	Sam68,mice
R177	T424	T419	nmod	C3H10T1,cells
R1770	T3076	T3072	prep	for,analyze
R1771	T3077	T3078	amod	skeletal,abnormalities
R1772	T3078	T3076	pobj	abnormalities,for
R1773	T3079	T3064	punct	.,decided
R1774	T3081	T3082	nsubjpass	Cohorts,euthanized
R1775	T3083	T3081	prep	of,Cohorts
R1776	T3084	T3085	nmod	Sam68,mice
R1777	T3085	T3083	pobj	mice,of
R1778	T3086	T3084	punct	+,Sam68
R1779	T3087	T3084	punct	/,Sam68
R178	T425	T424	punct	/,C3H10T1
R1780	T3088	T3084	punct	+,Sam68
R1781	T3089	T3084	cc	and,Sam68
R1782	T3090	T3084	conj	Sam68,Sam68
R1783	T3091	T3090	punct	−,Sam68
R1784	T3092	T3090	punct	/,Sam68
R1785	T3093	T3090	punct	−,Sam68
R1786	T3094	T3082	auxpass	were,euthanized
R1787	T3095	T3082	prep	at,euthanized
R1788	T3096	T3097	nummod	4,months
R1789	T3097	T3095	pobj	months,at
R179	T426	T424	nummod	2,C3H10T1
R1790	T3098	T3096	cc	and,4
R1791	T3099	T3096	conj	12,4
R1792	T3100	T3097	prep	of,months
R1793	T3101	T3100	pobj	age,of
R1794	T3102	T3082	prep	for,euthanized
R1795	T3103	T3104	amod	skeletal,phenotyping
R1796	T3104	T3102	pobj	phenotyping,for
R1797	T3105	T3082	punct	.,euthanized
R1798	T3107	T3108	aux	To,minimize
R1799	T3108	T3109	advcl	minimize,selected
R18	T254	T255	det	The,substrate
R180	T427	T407	prep	in,resulted
R1800	T3110	T3108	dobj	differences,minimize
R1801	T3111	T3110	prep	in,differences
R1802	T3112	T3113	det	the,phenotype
R1803	T3113	T3111	pobj	phenotype,in
R1804	T3114	T3113	compound	bone,phenotype
R1805	T3115	T3116	dep	that,arise
R1806	T3116	T3110	relcl	arise,differences
R1807	T3117	T3116	aux	might,arise
R1808	T3118	T3116	advcl	secondary,arise
R1809	T3119	T3118	prep	to,secondary
R181	T428	T429	advmod	more,pronounced
R1810	T3120	T3121	nmod	gender,differences
R1811	T3121	T3119	pobj	differences,to
R1812	T3122	T3120	cc	or,gender
R1813	T3123	T3120	conj	weight,gender
R1814	T3124	T3109	punct	", ",selected
R1815	T3125	T3109	nsubj	we,selected
R1816	T3126	T3127	npadvmod	age,matched
R1817	T3127	T3129	amod	matched,mice
R1818	T3128	T3127	punct	-,matched
R1819	T3129	T3109	dobj	mice,selected
R182	T429	T430	amod	pronounced,expression
R1820	T3130	T3129	amod	female,mice
R1821	T3131	T3109	prep	for,selected
R1822	T3132	T3133	det	these,analyses
R1823	T3133	T3131	pobj	analyses,for
R1824	T3134	T3109	punct	.,selected
R1825	T3136	T3137	det	The,mice
R1826	T3137	T3139	nsubj	mice,demonstrated
R1827	T3138	T3137	amod	female,mice
R1828	T3140	T3141	amod	similar,increases
R1829	T3141	T3139	dobj	increases,demonstrated
R183	T430	T427	pobj	expression,in
R1830	T3142	T3141	prep	in,increases
R1831	T3143	T3144	compound	body,weight
R1832	T3144	T3142	pobj	weight,in
R1833	T3145	T3146	punct	", ",weighed
R1834	T3146	T3141	parataxis	weighed,increases
R1835	T3147	T3146	mark	although,weighed
R1836	T3148	T3149	nummod	4,month
R1837	T3149	T3151	npadvmod	month,old
R1838	T3150	T3149	punct	-,month
R1839	T3151	T3153	amod	old,mice
R184	T431	T430	prep	of,expression
R1840	T3152	T3151	punct	-,old
R1841	T3153	T3146	nsubj	mice,weighed
R1842	T3154	T3153	nmod	Sam68,mice
R1843	T3155	T3154	punct	−,Sam68
R1844	T3156	T3154	punct	/,Sam68
R1845	T3157	T3154	punct	−,Sam68
R1846	T3158	T3159	amod	less,than
R1847	T3159	T3146	prep	than,weighed
R1848	T3160	T3161	nmod	Sam68,mice
R1849	T3161	T3159	pobj	mice,than
R185	T432	T433	det	the,marker
R1850	T3162	T3160	punct	+,Sam68
R1851	T3163	T3160	punct	/,Sam68
R1852	T3164	T3160	punct	+,Sam68
R1853	T3165	T3141	punct	", ",increases
R1854	T3166	T3141	cc	and,increases
R1855	T3167	T3168	amod	similar,changes
R1856	T3168	T3141	conj	changes,increases
R1857	T3169	T3168	prep	in,changes
R1858	T3170	T3171	compound	bone,lengths
R1859	T3171	T3169	pobj	lengths,in
R186	T433	T431	pobj	marker,of
R1860	T3172	T3168	prep	in,changes
R1861	T3173	T3174	det	the,skeleton
R1862	T3174	T3172	pobj	skeleton,in
R1863	T3175	T3174	amod	axial,skeleton
R1864	T3176	T3175	cc	and,axial
R1865	T3177	T3175	conj	appendicular,axial
R1866	T3178	T3168	prep	between,changes
R1867	T3179	T3180	nummod	4,months
R1868	T3180	T3178	pobj	months,between
R1869	T3181	T3179	cc	and,4
R187	T434	T433	amod	mature,marker
R1870	T3182	T3179	conj	12,4
R1871	T3183	T3180	prep	of,months
R1872	T3184	T3183	pobj	age,of
R1873	T3185	T3186	punct	(,Table
R1874	T3186	T3139	parataxis	Table,demonstrated
R1875	T3187	T3186	nummod	2,Table
R1876	T3188	T3186	punct	),Table
R1877	T3189	T3139	punct	.,demonstrated
R1878	T3191	T3192	compound	Faxitron,radiography
R1879	T3192	T3193	nsubj	radiography,revealed
R188	T435	T433	compound	osteoblast,marker
R1880	T3194	T3195	punct	(,Corporation
R1881	T3195	T3192	parataxis	Corporation,radiography
R1882	T3196	T3195	compound	Faxitron,Corporation
R1883	T3197	T3198	compound	X,ray
R1884	T3198	T3195	compound	ray,Corporation
R1885	T3199	T3198	punct	-,ray
R1886	T3200	T3195	punct	", ",Corporation
R1887	T3201	T3195	npadvmod	Wheeling,Corporation
R1888	T3202	T3195	punct	", ",Corporation
R1889	T3203	T3195	npadvmod	Illinois,Corporation
R189	T436	T433	appos	osteocalcin,marker
R1890	T3204	T3195	punct	", ",Corporation
R1891	T3205	T3206	compound	United,States
R1892	T3206	T3195	npadvmod	States,Corporation
R1893	T3207	T3195	punct	),Corporation
R1894	T3208	T3209	punct	(,3A
R1895	T3209	T3192	parataxis	3A,radiography
R1896	T3210	T3209	compound	Figure,3A
R1897	T3211	T3209	punct	),3A
R1898	T3212	T3192	cc	and,radiography
R1899	T3213	T3214	amod	micro–computed,tomography
R19	T255	T257	nsubj	substrate,is
R190	T437	T438	advmod	when,induced
R1900	T3214	T3192	conj	tomography,radiography
R1901	T3215	T3216	punct	(,CT
R1902	T3216	T3214	parataxis	CT,tomography
R1903	T3217	T3216	punct	),CT
R1904	T3218	T3219	punct	(,3B
R1905	T3219	T3214	parataxis	3B,tomography
R1906	T3220	T3219	compound	Figure,3B
R1907	T3221	T3219	punct	),3B
R1908	T3222	T3223	amod	significant,thinning
R1909	T3223	T3193	dobj	thinning,revealed
R191	T438	T407	advcl	induced,resulted
R1910	T3224	T3223	amod	cortical,thinning
R1911	T3225	T3226	punct	(,arrow
R1912	T3226	T3223	parataxis	arrow,thinning
R1913	T3227	T3226	punct	),arrow
R1914	T3228	T3223	cc	and,thinning
R1915	T3229	T3230	det	a,reduction
R1916	T3230	T3223	conj	reduction,thinning
R1917	T3231	T3230	prep	in,reduction
R1918	T3232	T3233	amod	metaphyseal,bone
R1919	T3233	T3231	pobj	bone,in
R192	T439	T438	nsubjpass	differentiation,induced
R1920	T3234	T3235	punct	(,asterisk
R1921	T3235	T3230	parataxis	asterisk,reduction
R1922	T3236	T3235	punct	),asterisk
R1923	T3237	T3223	prep	in,thinning
R1924	T3238	T3239	det	the,femora
R1925	T3239	T3237	pobj	femora,in
R1926	T3240	T3239	amod	distal,femora
R1927	T3241	T3239	prep	of,femora
R1928	T3242	T3243	nummod	12,month
R1929	T3243	T3245	npadvmod	month,old
R193	T440	T438	auxpass	was,induced
R1930	T3244	T3243	punct	-,month
R1931	T3245	T3247	amod	old,mice
R1932	T3246	T3245	punct	-,old
R1933	T3247	T3241	pobj	mice,of
R1934	T3248	T3247	nmod	Sam68,mice
R1935	T3249	T3248	punct	+,Sam68
R1936	T3250	T3248	punct	/,Sam68
R1937	T3251	T3248	punct	+,Sam68
R1938	T3252	T3223	prep	compared,thinning
R1939	T3253	T3252	prep	with,compared
R194	T441	T438	prep	with,induced
R1940	T3254	T3255	nummod	4,month
R1941	T3255	T3257	npadvmod	month,old
R1942	T3256	T3255	punct	-,month
R1943	T3257	T3259	amod	old,mice
R1944	T3258	T3257	punct	-,old
R1945	T3259	T3253	pobj	mice,with
R1946	T3260	T3259	prep	of,mice
R1947	T3261	T3262	preconj	either,genotype
R1948	T3262	T3260	pobj	genotype,of
R1949	T3263	T3259	cc	and,mice
R195	T442	T443	nmod	bone,protein
R1950	T3264	T3265	nummod	12,month
R1951	T3265	T3267	npadvmod	month,old
R1952	T3266	T3265	punct	-,month
R1953	T3267	T3269	amod	old,mice
R1954	T3268	T3267	punct	-,old
R1955	T3269	T3259	conj	mice,mice
R1956	T3270	T3269	nmod	Sam68,mice
R1957	T3271	T3270	punct	−,Sam68
R1958	T3272	T3270	punct	/,Sam68
R1959	T3273	T3270	punct	−,Sam68
R196	T443	T441	pobj	protein,with
R1960	T3274	T3193	punct	.,revealed
R1961	T3276	T3277	amod	Similar,reductions
R1962	T3277	T3278	nsubjpass	reductions,shown
R1963	T3279	T3277	prep	in,reductions
R1964	T3280	T3281	amod	trabecular,bone
R1965	T3281	T3279	pobj	bone,in
R1966	T3282	T3278	auxpass	were,shown
R1967	T3283	T3278	prep	by,shown
R1968	T3284	T3283	pobj	Faxitron,by
R1969	T3285	T3284	cc	and,Faxitron
R197	T444	T443	amod	morphogenetic,protein
R1970	T3286	T3284	conj	micro-CT,Faxitron
R1971	T3287	T3278	prep	in,shown
R1972	T3288	T3289	det	the,vertebrae
R1973	T3289	T3287	pobj	vertebrae,in
R1974	T3290	T3289	amod	fifth,vertebrae
R1975	T3291	T3289	compound	lumbar,vertebrae
R1976	T3292	T3289	prep	of,vertebrae
R1977	T3293	T3294	det	the,mice
R1978	T3294	T3292	pobj	mice,of
R1979	T3295	T3296	nummod	12,month
R198	T445	T443	punct	-,protein
R1980	T3296	T3298	npadvmod	month,old
R1981	T3297	T3296	punct	-,month
R1982	T3298	T3294	amod	old,mice
R1983	T3299	T3298	punct	-,old
R1984	T3300	T3294	nmod	Sam68,mice
R1985	T3301	T3300	punct	+,Sam68
R1986	T3302	T3300	punct	/,Sam68
R1987	T3303	T3300	punct	+,Sam68
R1988	T3304	T3287	cc	but,in
R1989	T3305	T3304	neg	not,but
R199	T446	T443	nummod	2,protein
R1990	T3306	T3287	conj	in,in
R1991	T3307	T3308	det	the,mice
R1992	T3308	T3306	pobj	mice,in
R1993	T3309	T3308	nmod	Sam68,mice
R1994	T3310	T3309	punct	−,Sam68
R1995	T3311	T3309	punct	/,Sam68
R1996	T3312	T3309	punct	−,Sam68
R1997	T3313	T3314	punct	(,data
R1998	T3314	T3278	meta	data,shown
R1999	T3315	T3314	amod	unpublished,data
R20	T256	T255	compound	Src,substrate
R200	T447	T407	punct	.,resulted
R2000	T3316	T3314	punct	),data
R2001	T3317	T3278	punct	.,shown
R2002	T3319	T3320	amod	Total,content
R2003	T3320	T3324	nsubj	content,increased
R2004	T3321	T3320	compound	body,content
R2005	T3322	T3320	compound	bone,content
R2006	T3323	T3320	compound	mineral,content
R2007	T3325	T3320	punct	(,content
R2008	T3326	T3320	appos	BMC,content
R2009	T3327	T3320	punct	),content
R201	T449	T450	nsubjpass	Cultures,induced
R2010	T3328	T3320	punct	", ",content
R2011	T3329	T3320	acl	quantified,content
R2012	T3330	T3329	prep	with,quantified
R2013	T3331	T3332	det	a,densitometer
R2014	T3332	T3330	pobj	densitometer,with
R2015	T3333	T3334	amod	Lunar,PIXImus
R2016	T3334	T3332	compound	PIXImus,densitometer
R2017	T3335	T3332	compound	mouse,densitometer
R2018	T3336	T3337	punct	(,Lunar
R2019	T3337	T3332	parataxis	Lunar,densitometer
R202	T451	T449	prep	of,Cultures
R2020	T3338	T3337	compound	GE,Lunar
R2021	T3339	T3337	punct	-,Lunar
R2022	T3340	T3337	punct	", ",Lunar
R2023	T3341	T3337	npadvmod	Madison,Lunar
R2024	T3342	T3337	punct	", ",Lunar
R2025	T3343	T3337	npadvmod	Wisconsin,Lunar
R2026	T3344	T3337	punct	", ",Lunar
R2027	T3345	T3346	compound	United,States
R2028	T3346	T3337	npadvmod	States,Lunar
R2029	T3347	T3337	punct	),Lunar
R203	T452	T453	compound	mouse,embryo
R2030	T3348	T3324	punct	", ",increased
R2031	T3349	T3324	prep	in,increased
R2032	T3350	T3351	preconj	both,Sam68
R2033	T3351	T3352	nmod	Sam68,mice
R2034	T3352	T3349	pobj	mice,in
R2035	T3353	T3351	punct	+,Sam68
R2036	T3354	T3351	punct	/,Sam68
R2037	T3355	T3351	punct	+,Sam68
R2038	T3356	T3351	cc	and,Sam68
R2039	T3357	T3351	conj	Sam68,Sam68
R204	T453	T454	compound	embryo,fibroblasts
R2040	T3358	T3357	punct	−,Sam68
R2041	T3359	T3357	punct	/,Sam68
R2042	T3360	T3357	punct	−,Sam68
R2043	T3361	T3352	prep	between,mice
R2044	T3362	T3363	nummod	4,months
R2045	T3363	T3361	pobj	months,between
R2046	T3364	T3363	cc	and,months
R2047	T3365	T3366	nummod	12,months
R2048	T3366	T3363	conj	months,months
R2049	T3367	T3363	prep	of,months
R205	T454	T451	pobj	fibroblasts,of
R2050	T3368	T3367	pobj	age,of
R2051	T3369	T3324	punct	", ",increased
R2052	T3370	T3324	cc	but,increased
R2053	T3371	T3372	advmod	only,reached
R2054	T3372	T3324	conj	reached,increased
R2055	T3373	T3372	dobj	significance,reached
R2056	T3374	T3372	prep	in,reached
R2057	T3375	T3376	det	the,mice
R2058	T3376	T3374	pobj	mice,in
R2059	T3377	T3376	nmod	Sam68,mice
R206	T455	T449	acl	generated,Cultures
R2060	T3378	T3377	punct	−,Sam68
R2061	T3379	T3377	punct	/,Sam68
R2062	T3380	T3377	punct	−,Sam68
R2063	T3381	T3382	punct	(,Table
R2064	T3382	T3372	parataxis	Table,reached
R2065	T3383	T3382	nummod	2,Table
R2066	T3384	T3382	punct	),Table
R2067	T3385	T3324	punct	.,increased
R2068	T3387	T3388	advmod	Similarly,seen
R2069	T3389	T3388	punct	", ",seen
R207	T456	T455	prep	from,generated
R2070	T3390	T3391	det	a,increase
R2071	T3391	T3388	nsubjpass	increase,seen
R2072	T3392	T3391	amod	greater,increase
R2073	T3393	T3391	prep	in,increase
R2074	T3394	T3393	pobj	BMC,in
R2075	T3395	T3388	auxpass	was,seen
R2076	T3396	T3388	prep	in,seen
R2077	T3397	T3398	det	the,femur
R2078	T3398	T3396	pobj	femur,in
R2079	T3399	T3398	cc	and,femur
R208	T457	T458	nmod	Sam68,littermates
R2080	T3400	T3398	conj	vertebra,femur
R2081	T3401	T3398	prep	in,femur
R2082	T3402	T3403	det	the,mice
R2083	T3403	T3401	pobj	mice,in
R2084	T3404	T3403	nmod	Sam68,mice
R2085	T3405	T3404	punct	−,Sam68
R2086	T3406	T3404	punct	/,Sam68
R2087	T3407	T3404	punct	−,Sam68
R2088	T3408	T3388	punct	.,seen
R2089	T3410	T3411	compound	Bone,mineral
R209	T458	T456	pobj	littermates,from
R2090	T3411	T3412	compound	mineral,density
R2091	T3412	T3413	nsubj	density,remained
R2092	T3414	T3412	punct	(,density
R2093	T3415	T3412	appos	BMD,density
R2094	T3416	T3413	punct	),remained
R2095	T3417	T3413	acomp	constant,remained
R2096	T3418	T3413	cc	or,remained
R2097	T3419	T3413	conj	decreased,remained
R2098	T3420	T3413	prep	in,remained
R2099	T3421	T3422	det	the,mice
R21	T257	T267	ccomp	is,remained
R210	T459	T457	punct	+,Sam68
R2100	T3422	T3420	pobj	mice,in
R2101	T3423	T3424	amod	wild,type
R2102	T3424	T3422	compound	type,mice
R2103	T3425	T3413	cc	but,remained
R2104	T3426	T3413	conj	increased,remained
R2105	T3427	T3426	advmod	significantly,increased
R2106	T3428	T3426	prep	in,increased
R2107	T3429	T3430	det	the,body
R2108	T3430	T3428	pobj	body,in
R2109	T3431	T3430	amod	total,body
R211	T460	T457	punct	/,Sam68
R2110	T3432	T3428	cc	and,in
R2111	T3433	T3428	conj	in,in
R2112	T3434	T3435	det	the,regions
R2113	T3435	T3433	pobj	regions,in
R2114	T3436	T3435	amod	femoral,regions
R2115	T3437	T3436	cc	and,femoral
R2116	T3438	T3436	conj	vertebral,femoral
R2117	T3439	T3435	prep	of,regions
R2118	T3440	T3439	pobj	interest,of
R2119	T3441	T3426	prep	in,increased
R212	T461	T457	punct	+,Sam68
R2120	T3442	T3443	det	the,Sam68
R2121	T3443	T3441	pobj	Sam68,in
R2122	T3444	T3443	punct	−,Sam68
R2123	T3445	T3443	punct	/,Sam68
R2124	T3446	T3443	punct	−,Sam68
R2125	T3447	T3448	punct	(,Table
R2126	T3448	T3426	parataxis	Table,increased
R2127	T3449	T3448	nummod	2,Table
R2128	T3450	T3448	punct	),Table
R2129	T3451	T3413	punct	.,remained
R213	T462	T457	cc	and,Sam68
R2130	T3453	T3454	det	These,data
R2131	T3454	T3455	nsubj	data,showed
R2132	T3456	T3457	mark	that,continued
R2133	T3457	T3455	ccomp	continued,showed
R2134	T3458	T3459	nmod	Sam68,mice
R2135	T3459	T3457	nsubj	mice,continued
R2136	T3460	T3458	punct	−,Sam68
R2137	T3461	T3458	punct	/,Sam68
R2138	T3462	T3458	punct	−,Sam68
R2139	T3463	T3464	aux	to,thrive
R214	T463	T457	conj	Sam68,Sam68
R2140	T3464	T3457	xcomp	thrive,continued
R2141	T3465	T3464	cc	and,thrive
R2142	T3466	T3464	conj	accrue,thrive
R2143	T3467	T3466	dobj	bone,accrue
R2144	T3468	T3466	prep	in,accrue
R2145	T3469	T3470	det	the,skeleton
R2146	T3470	T3468	pobj	skeleton,in
R2147	T3471	T3470	amod	axial,skeleton
R2148	T3472	T3471	cc	and,axial
R2149	T3473	T3471	conj	appendicular,axial
R215	T464	T463	punct	−,Sam68
R2150	T3474	T3464	prep	for,thrive
R2151	T3475	T3476	amod	longer,12
R2152	T3476	T3478	nummod	12,months
R2153	T3477	T3476	quantmod	than,12
R2154	T3478	T3474	pobj	months,for
R2155	T3479	T3455	punct	.,showed
R2156	T3481	T3482	nsubj	This,was
R2157	T3483	T3482	prep	in,was
R2158	T3484	T3483	pobj	contrast,in
R2159	T3485	T3484	prep	to,contrast
R216	T465	T463	punct	/,Sam68
R2160	T3486	T3487	det	the,situation
R2161	T3487	T3485	pobj	situation,to
R2162	T3488	T3487	prep	in,situation
R2163	T3489	T3490	npadvmod	age,matched
R2164	T3490	T3492	amod	matched,controls
R2165	T3491	T3490	punct	-,matched
R2166	T3492	T3488	pobj	controls,in
R2167	T3493	T3492	punct	", ",controls
R2168	T3494	T3492	compound	littermate,controls
R2169	T3495	T3496	prep	in,lost
R217	T466	T463	punct	−,Sam68
R2170	T3496	T3487	relcl	lost,situation
R2171	T3497	T3495	pobj	which,in
R2172	T3498	T3499	det	a,amount
R2173	T3499	T3496	nsubjpass	amount,lost
R2174	T3500	T3499	amod	significant,amount
R2175	T3501	T3499	prep	of,amount
R2176	T3502	T3501	pobj	bone,of
R2177	T3503	T3496	auxpass	was,lost
R2178	T3504	T3496	prep	over,lost
R2179	T3505	T3506	det	the,timeframe
R218	T467	T450	auxpass	were,induced
R2180	T3506	T3504	pobj	timeframe,over
R2181	T3507	T3506	amod	same,timeframe
R2182	T3508	T3482	punct	.,was
R2183	T3587	T3588	advmod	Three,Dimensional
R2184	T3588	T3590	amod	Dimensional,Architecture
R2185	T3589	T3588	punct	-,Dimensional
R2186	T3590	T3591	nsubjpass	Architecture,Preserved
R2187	T3592	T3590	prep	of,Architecture
R2188	T3593	T3592	pobj	Bone,of
R2189	T3594	T3591	auxpass	Is,Preserved
R219	T468	T469	aux	to,differentiate
R2190	T3595	T3591	prep	in,Preserved
R2191	T3596	T3597	amod	Aged,Mice
R2192	T3597	T3595	pobj	Mice,in
R2193	T3598	T3597	nmod	Sam68,Mice
R2194	T3599	T3598	punct	−,Sam68
R2195	T3600	T3598	punct	/,Sam68
R2196	T3601	T3598	punct	−,Sam68
R2197	T3603	T3604	compound	Bone,loss
R2198	T3604	T3605	nsubj	loss,are
R2199	T3606	T3604	cc	and,loss
R22	T258	T255	acl	associated,substrate
R220	T469	T450	advcl	differentiate,induced
R2200	T3607	T3608	amod	compromised,architecture
R2201	T3608	T3604	conj	architecture,loss
R2202	T3609	T3610	amod	characteristic,features
R2203	T3610	T3605	attr	features,are
R2204	T3611	T3610	prep	of,features
R2205	T3612	T3613	det	the,skeletons
R2206	T3613	T3611	pobj	skeletons,of
R2207	T3614	T3613	prep	of,skeletons
R2208	T3615	T3616	amod	aged,mice
R2209	T3616	T3614	pobj	mice,of
R221	T470	T469	prep	into,differentiate
R2210	T3617	T3616	nmod	C57BL,mice
R2211	T3618	T3617	punct	/,C57BL
R2212	T3619	T3617	nummod	6,C57BL
R2213	T3620	T3605	cc	and,are
R2214	T3621	T3605	conj	resemble,are
R2215	T3622	T3623	det	the,features
R2216	T3623	T3621	dobj	features,resemble
R2217	T3624	T3623	amod	clinical,features
R2218	T3625	T3623	prep	of,features
R2219	T3626	T3627	npadvmod	age,related
R222	T471	T470	pobj	adipocytes,into
R2220	T3627	T3629	amod	related,loss
R2221	T3628	T3627	punct	-,related
R2222	T3629	T3625	pobj	loss,of
R2223	T3630	T3629	compound	bone,loss
R2224	T3631	T3629	prep	in,loss
R2225	T3632	T3631	pobj	humans,in
R2226	T3633	T3634	dep	that,predispose
R2227	T3634	T3623	relcl	predispose,features
R2228	T3635	T3634	aux	can,predispose
R2229	T3636	T3637	det	an,individual
R223	T472	T450	prep	with,induced
R2230	T3637	T3634	dobj	individual,predispose
R2231	T3638	T3634	prep	to,predispose
R2232	T3639	T3638	pobj	fracture,to
R2233	T3640	T3605	punct	.,are
R2234	T3642	T3643	nsubj	Quantification,confirmed
R2235	T3644	T3642	prep	of,Quantification
R2236	T3645	T3646	det	the,data
R2237	T3646	T3644	pobj	data,of
R2238	T3647	T3646	compound	micro-CT,data
R2239	T3648	T3646	acl	shown,data
R224	T473	T474	compound	culture,medium
R2240	T3649	T3648	prep	in,shown
R2241	T3650	T3651	compound	Figure,3B
R2242	T3651	T3649	pobj	3B,in
R2243	T3652	T3653	det	the,reduction
R2244	T3653	T3643	dobj	reduction,confirmed
R2245	T3654	T3653	prep	in,reduction
R2246	T3655	T3656	compound	bone,volume
R2247	T3656	T3654	pobj	volume,in
R2248	T3657	T3653	prep	compared,reduction
R2249	T3658	T3657	prep	with,compared
R225	T474	T472	pobj	medium,with
R2250	T3659	T3660	compound	tissue,volume
R2251	T3660	T3658	pobj	volume,with
R2252	T3661	T3662	punct	(,4A
R2253	T3662	T3660	parataxis	4A,volume
R2254	T3663	T3664	compound	BV,TV
R2255	T3664	T3662	dep	TV,4A
R2256	T3665	T3664	punct	/,TV
R2257	T3666	T3662	punct	;,4A
R2258	T3667	T3662	compound	Figure,4A
R2259	T3668	T3662	punct	", ",4A
R226	T475	T474	acl	containing,medium
R2260	T3669	T3662	amod	left,4A
R2261	T3670	T3662	punct	),4A
R2262	T3671	T3660	prep	in,volume
R2263	T3672	T3673	det	the,mice
R2264	T3673	T3671	pobj	mice,in
R2265	T3674	T3675	nummod	12,month
R2266	T3675	T3677	npadvmod	month,old
R2267	T3676	T3675	punct	-,month
R2268	T3677	T3673	amod	old,mice
R2269	T3678	T3677	punct	-,old
R227	T476	T475	dobj	pioglitazone,containing
R2270	T3679	T3673	nmod	Sam68,mice
R2271	T3680	T3679	punct	+,Sam68
R2272	T3681	T3679	punct	/,Sam68
R2273	T3682	T3679	punct	+,Sam68
R2274	T3683	T3684	punct	(,bars
R2275	T3684	T3673	parataxis	bars,mice
R2276	T3685	T3684	amod	hatched,bars
R2277	T3686	T3684	punct	),bars
R2278	T3687	T3660	prep	compared,volume
R2279	T3688	T3687	prep	with,compared
R228	T477	T476	cc	and,pioglitazone
R2280	T3689	T3690	nummod	4,month
R2281	T3690	T3692	npadvmod	month,old
R2282	T3691	T3690	punct	-,month
R2283	T3692	T3694	amod	old,mice
R2284	T3693	T3692	punct	-,old
R2285	T3694	T3688	pobj	mice,with
R2286	T3695	T3694	prep	of,mice
R2287	T3696	T3697	det	both,genotypes
R2288	T3697	T3695	pobj	genotypes,of
R2289	T3698	T3699	punct	(,bars
R229	T478	T479	det	the,fibroblasts
R2290	T3699	T3694	parataxis	bars,mice
R2291	T3700	T3699	amod	solid,bars
R2292	T3701	T3699	punct	),bars
R2293	T3702	T3694	cc	and,mice
R2294	T3703	T3704	nummod	12,month
R2295	T3704	T3706	npadvmod	month,old
R2296	T3705	T3704	punct	-,month
R2297	T3706	T3708	amod	old,mice
R2298	T3707	T3706	punct	-,old
R2299	T3708	T3694	conj	mice,mice
R23	T259	T258	prep	in,associated
R230	T479	T476	conj	fibroblasts,pioglitazone
R2300	T3709	T3708	nmod	Sam68,mice
R2301	T3710	T3709	punct	−,Sam68
R2302	T3711	T3709	punct	/,Sam68
R2303	T3712	T3709	punct	−,Sam68
R2304	T3713	T3714	punct	(,bars
R2305	T3714	T3708	parataxis	bars,mice
R2306	T3715	T3714	amod	stippled,bars
R2307	T3716	T3714	punct	),bars
R2308	T3717	T3643	punct	.,confirmed
R2309	T3719	T3720	nsubjpass	This,associated
R231	T480	T479	nmod	Sam68,fibroblasts
R2310	T3721	T3720	auxpass	was,associated
R2311	T3722	T3720	prep	with,associated
R2312	T3723	T3724	det	a,increase
R2313	T3724	T3722	pobj	increase,with
R2314	T3725	T3724	amod	significant,increase
R2315	T3726	T3727	punct	(,denoted
R2316	T3727	T3724	parataxis	denoted,increase
R2317	T3728	T3729	nsubj	p,0.01
R2318	T3729	T3727	ccomp	0.01,denoted
R2319	T3730	T3729	punct	<,0.01
R232	T481	T480	punct	−,Sam68
R2320	T3731	T3727	punct	;,denoted
R2321	T3732	T3727	agent	by,denoted
R2322	T3733	T3734	det	the,asterisks
R2323	T3734	T3732	pobj	asterisks,by
R2324	T3735	T3727	punct	),denoted
R2325	T3736	T3724	prep	in,increase
R2326	T3737	T3738	det	the,index
R2327	T3738	T3736	pobj	index,in
R2328	T3739	T3738	compound	structure,index
R2329	T3740	T3738	compound	model,index
R233	T482	T480	punct	/,Sam68
R2330	T3741	T3738	punct	", ",index
R2331	T3742	T3743	dep	which,measures
R2332	T3743	T3738	relcl	measures,index
R2333	T3744	T3745	det	the,ratio
R2334	T3745	T3743	dobj	ratio,measures
R2335	T3746	T3745	prep	of,ratio
R2336	T3747	T3748	npadvmod	plate,like
R2337	T3748	T3746	pobj	like,of
R2338	T3749	T3748	punct	-,like
R2339	T3750	T3748	prep	to,like
R234	T483	T480	punct	−,Sam68
R2340	T3751	T3752	npadvmod	rod,like
R2341	T3752	T3750	pobj	like,to
R2342	T3753	T3752	punct	-,like
R2343	T3754	T3748	appos	structures,like
R2344	T3755	T3756	punct	(,4A
R2345	T3756	T3743	parataxis	4A,measures
R2346	T3757	T3756	compound	Figure,4A
R2347	T3758	T3756	punct	", ",4A
R2348	T3759	T3756	appos	right,4A
R2349	T3760	T3756	punct	),4A
R235	T484	T479	compound	mouse,fibroblasts
R2350	T3761	T3720	punct	.,associated
R2351	T3763	T3764	det	A,increase
R2352	T3764	T3766	nsubj	increase,was
R2353	T3765	T3764	amod	quantifiable,increase
R2354	T3767	T3764	prep	in,increase
R2355	T3768	T3769	det	the,separation
R2356	T3769	T3767	pobj	separation,in
R2357	T3770	T3769	nmod	mean,separation
R2358	T3771	T3769	amod	trabecular,separation
R2359	T3772	T3769	punct	(,separation
R236	T485	T479	compound	embryo,fibroblasts
R2360	T3773	T3769	appos	Tb.Sp,separation
R2361	T3774	T3769	punct	),separation
R2362	T3775	T3776	punct	(,data
R2363	T3776	T3769	parataxis	data,separation
R2364	T3777	T3776	amod	unpublished,data
R2365	T3778	T3776	punct	),data
R2366	T3779	T3764	prep	in,increase
R2367	T3780	T3781	nummod	12,month
R2368	T3781	T3783	npadvmod	month,old
R2369	T3782	T3781	punct	-,month
R237	T486	T479	acl	shown,fibroblasts
R2370	T3783	T3785	amod	old,mice
R2371	T3784	T3783	punct	-,old
R2372	T3785	T3779	pobj	mice,in
R2373	T3786	T3785	nmod	Sam68,mice
R2374	T3787	T3786	punct	+,Sam68
R2375	T3788	T3786	punct	/,Sam68
R2376	T3789	T3786	punct	+,Sam68
R2377	T3790	T3766	prep	due,was
R2378	T3791	T3790	pcomp	to,due
R2379	T3792	T3793	det	an,increase
R238	T487	T488	aux	to,have
R2380	T3793	T3790	pobj	increase,due
R2381	T3794	T3793	prep	in,increase
R2382	T3795	T3796	det	the,percentage
R2383	T3796	T3794	pobj	percentage,in
R2384	T3797	T3796	prep	of,percentage
R2385	T3798	T3797	pobj	spaces,of
R2386	T3799	T3798	acl	falling,spaces
R2387	T3800	T3799	prep	in,falling
R2388	T3801	T3802	det	the,range
R2389	T3802	T3800	pobj	range,in
R239	T488	T486	xcomp	have,shown
R2390	T3803	T3802	prep	of,range
R2391	T3804	T3805	quantmod	350,700
R2392	T3805	T3807	nummod	700,μm
R2393	T3806	T3805	quantmod	to,700
R2394	T3807	T3803	pobj	μm,of
R2395	T3808	T3809	punct	(,4B
R2396	T3809	T3766	parataxis	4B,was
R2397	T3810	T3809	compound	Figure,4B
R2398	T3811	T3809	punct	", ",4B
R2399	T3812	T3813	amod	red,line
R24	T260	T259	pobj	mitosis,in
R240	T489	T490	amod	impaired,differentiation
R2400	T3813	T3809	appos	line,4B
R2401	T3814	T3813	amod	hatched,line
R2402	T3815	T3809	punct	),4B
R2403	T3816	T3766	punct	.,was
R2404	T3818	T3819	amod	Trabecular,thickness
R2405	T3819	T3820	nsubj	thickness,remained
R2406	T3821	T3820	acomp	constant,remained
R2407	T3822	T3820	prep	among,remained
R2408	T3823	T3824	det	the,groups
R2409	T3824	T3822	pobj	groups,among
R241	T490	T488	dobj	differentiation,have
R2410	T3825	T3824	amod	different,groups
R2411	T3826	T3824	prep	of,groups
R2412	T3827	T3826	pobj	mice,of
R2413	T3828	T3829	punct	(,data
R2414	T3829	T3820	meta	data,remained
R2415	T3830	T3829	amod	unpublished,data
R2416	T3831	T3829	punct	),data
R2417	T3832	T3820	punct	.,remained
R2418	T3834	T3835	prep	In,meant
R2419	T3836	T3834	pobj	effect,In
R242	T491	T490	compound	adipocyte,differentiation
R2420	T3837	T3835	punct	", ",meant
R2421	T3838	T3835	nsubj	this,meant
R2422	T3839	T3840	mark	that,were
R2423	T3840	T3835	ccomp	were,meant
R2424	T3841	T3840	expl	there,were
R2425	T3842	T3843	amod	fewer,trabeculae
R2426	T3843	T3840	attr	trabeculae,were
R2427	T3844	T3843	punct	", ",trabeculae
R2428	T3845	T3846	advmod	rather,than
R2429	T3846	T3843	cc	than,trabeculae
R243	T492	T450	punct	.,induced
R2430	T3847	T3848	amod	equivalent,numbers
R2431	T3848	T3843	conj	numbers,trabeculae
R2432	T3849	T3848	prep	of,numbers
R2433	T3850	T3851	amod	thin,trabeculae
R2434	T3851	T3849	pobj	trabeculae,of
R2435	T3852	T3843	punct	", ",trabeculae
R2436	T3853	T3843	prep	in,trabeculae
R2437	T3854	T3855	det	the,mice
R2438	T3855	T3853	pobj	mice,in
R2439	T3856	T3857	nummod	12,month
R244	T494	T495	advmod	Furthermore,shown
R2440	T3857	T3859	npadvmod	month,old
R2441	T3858	T3857	punct	-,month
R2442	T3859	T3855	amod	old,mice
R2443	T3860	T3859	punct	-,old
R2444	T3861	T3855	nmod	Sam68,mice
R2445	T3862	T3861	punct	+,Sam68
R2446	T3863	T3861	punct	/,Sam68
R2447	T3864	T3861	punct	+,Sam68
R2448	T3865	T3843	prep	compared,trabeculae
R2449	T3866	T3865	prep	with,compared
R245	T496	T495	punct	", ",shown
R2450	T3867	T3866	pobj	any,with
R2451	T3868	T3867	prep	of,any
R2452	T3869	T3870	det	the,groups
R2453	T3870	T3868	pobj	groups,of
R2454	T3871	T3870	amod	other,groups
R2455	T3872	T3870	prep	of,groups
R2456	T3873	T3872	pobj	mice,of
R2457	T3874	T3835	punct	.,meant
R2458	T4163	T4164	compound	Bone,Remodeling
R2459	T4164	T4165	nsubjpass	Remodeling,Preserved
R246	T497	T498	advmod	in,vivo
R2460	T4166	T4165	auxpass	Is,Preserved
R2461	T4167	T4165	prep	in,Preserved
R2462	T4168	T4169	amod	Aged,Mice
R2463	T4169	T4167	pobj	Mice,in
R2464	T4170	T4169	nmod	Sam68,Mice
R2465	T4171	T4170	punct	−,Sam68
R2466	T4172	T4170	punct	/,Sam68
R2467	T4173	T4170	punct	−,Sam68
R2468	T4175	T4176	aux	To,define
R2469	T4176	T4178	advcl	define,prepared
R247	T498	T495	advmod	vivo,shown
R2470	T4177	T4176	advmod	further,define
R2471	T4179	T4180	det	the,mechanisms
R2472	T4180	T4176	dobj	mechanisms,define
R2473	T4181	T4180	acl	involved,mechanisms
R2474	T4182	T4181	prep	in,involved
R2475	T4183	T4184	det	the,preservation
R2476	T4184	T4182	pobj	preservation,in
R2477	T4185	T4184	prep	of,preservation
R2478	T4186	T4187	compound	bone,mass
R2479	T4187	T4185	pobj	mass,of
R248	T499	T495	nsubjpass	it,shown
R2480	T4188	T4184	prep	in,preservation
R2481	T4189	T4190	amod	aged,mice
R2482	T4190	T4188	pobj	mice,in
R2483	T4191	T4190	nmod	Sam68,mice
R2484	T4192	T4191	punct	−,Sam68
R2485	T4193	T4191	punct	/,Sam68
R2486	T4194	T4191	punct	−,Sam68
R2487	T4195	T4178	punct	", ",prepared
R2488	T4196	T4178	nsubj	we,prepared
R2489	T4197	T4178	dobj	sections,prepared
R249	T500	T495	auxpass	was,shown
R2490	T4198	T4178	prep	from,prepared
R2491	T4199	T4200	npadvmod	plastic,embedded
R2492	T4200	T4202	amod	embedded,femora
R2493	T4201	T4200	punct	-,embedded
R2494	T4202	T4198	pobj	femora,from
R2495	T4203	T4202	cc	and,femora
R2496	T4204	T4202	conj	tibia,femora
R2497	T4205	T4206	aux	to,evaluate
R2498	T4206	T4178	advcl	evaluate,prepared
R2499	T4207	T4208	nmod	osteoblast,activity
R25	T261	T260	prep	of,mitosis
R250	T501	T502	mark	that,had
R2500	T4208	T4206	dobj	activity,evaluate
R2501	T4209	T4207	cc	and,osteoblast
R2502	T4210	T4207	conj	osteoclast,osteoblast
R2503	T4211	T4212	punct	(,Figure
R2504	T4212	T4178	parataxis	Figure,prepared
R2505	T4213	T4212	nummod	5,Figure
R2506	T4214	T4212	punct	),Figure
R2507	T4215	T4178	punct	.,prepared
R2508	T4217	T4218	advmod	In,situ
R2509	T4218	T4219	amod	situ,staining
R251	T502	T495	ccomp	had,shown
R2510	T4219	T4222	nsubjpass	staining,used
R2511	T4220	T4219	nmod	enzyme,staining
R2512	T4221	T4219	amod	histochemical,staining
R2513	T4223	T4219	prep	for,staining
R2514	T4224	T4225	nmod	alkaline,phosphatase
R2515	T4225	T4226	nmod	phosphatase,activity
R2516	T4226	T4223	pobj	activity,for
R2517	T4227	T4225	punct	(,phosphatase
R2518	T4228	T4225	appos	ALP,phosphatase
R2519	T4229	T4230	punct	;,stain
R252	T503	T502	nsubj	sections,had
R2520	T4230	T4228	parataxis	stain,ALP
R2521	T4231	T4230	amod	brown,stain
R2522	T4232	T4230	punct	),stain
R2523	T4233	T4222	auxpass	was,used
R2524	T4234	T4222	prep	as,used
R2525	T4235	T4236	det	a,biomarker
R2526	T4236	T4234	pobj	biomarker,as
R2527	T4237	T4236	prep	for,biomarker
R2528	T4238	T4237	pobj	osteoblasts,for
R2529	T4239	T4238	cc	and,osteoblasts
R253	T504	T503	prep	of,sections
R2530	T4240	T4241	npadvmod	tartrate,resistant
R2531	T4241	T4243	amod	resistant,activity
R2532	T4242	T4241	punct	-,resistant
R2533	T4243	T4238	conj	activity,osteoblasts
R2534	T4244	T4243	nmod	ALP,activity
R2535	T4245	T4246	punct	(,stain
R2536	T4246	T4243	parataxis	stain,activity
R2537	T4247	T4248	npadvmod	tartrate,resistant
R2538	T4248	T4250	amod	resistant,phosphatase
R2539	T4249	T4248	punct	-,resistant
R254	T505	T504	pobj	bone,of
R2540	T4250	T4246	dep	phosphatase,stain
R2541	T4251	T4250	compound	acid,phosphatase
R2542	T4252	T4250	punct	[,phosphatase
R2543	T4253	T4250	appos	TRAP,phosphatase
R2544	T4254	T4246	punct	],stain
R2545	T4255	T4246	punct	;,stain
R2546	T4256	T4246	amod	red,stain
R2547	T4257	T4246	punct	),stain
R2548	T4258	T4222	prep	as,used
R2549	T4259	T4260	det	a,marker
R255	T506	T503	prep	from,sections
R2550	T4260	T4258	pobj	marker,as
R2551	T4261	T4260	prep	for,marker
R2552	T4262	T4261	pobj	osteoclasts,for
R2553	T4263	T4222	punct	.,used
R2554	T4265	T4266	amod	Little,difference
R2555	T4266	T4267	nsubjpass	difference,seen
R2556	T4268	T4267	auxpass	was,seen
R2557	T4269	T4267	prep	between,seen
R2558	T4270	T4271	nmod	Sam68,mice
R2559	T4271	T4269	pobj	mice,between
R256	T507	T508	nummod	12,month
R2560	T4272	T4270	punct	+,Sam68
R2561	T4273	T4270	punct	/,Sam68
R2562	T4274	T4270	punct	+,Sam68
R2563	T4275	T4276	punct	(,5A
R2564	T4276	T4270	parataxis	5A,Sam68
R2565	T4277	T4276	compound	Figure,5A
R2566	T4278	T4276	cc	and,5A
R2567	T4279	T4276	conj	5B,5A
R2568	T4280	T4276	punct	),5A
R2569	T4281	T4270	cc	and,Sam68
R257	T508	T510	npadvmod	month,old
R2570	T4282	T4270	conj	Sam68,Sam68
R2571	T4283	T4282	punct	−,Sam68
R2572	T4284	T4282	punct	/,Sam68
R2573	T4285	T4282	punct	−,Sam68
R2574	T4286	T4287	punct	(,5C
R2575	T4287	T4282	parataxis	5C,Sam68
R2576	T4288	T4287	compound	Figure,5C
R2577	T4289	T4287	cc	and,5C
R2578	T4290	T4287	conj	5D,5C
R2579	T4291	T4287	punct	),5C
R258	T509	T508	punct	-,month
R2580	T4292	T4267	prep	at,seen
R2581	T4293	T4294	nummod	4,months
R2582	T4294	T4292	pobj	months,at
R2583	T4295	T4294	prep	of,months
R2584	T4296	T4295	pobj	age,of
R2585	T4297	T4267	punct	.,seen
R2586	T4299	T4300	npadvmod	ALP,positive
R2587	T4300	T4305	amod	positive,cells
R2588	T4301	T4299	punct	-,ALP
R2589	T4302	T4299	cc	and,ALP
R259	T510	T512	amod	old,mice
R2590	T4303	T4299	conj	TRAP,ALP
R2591	T4304	T4300	punct	-,positive
R2592	T4305	T4306	nsubjpass	cells,reduced
R2593	T4307	T4306	auxpass	were,reduced
R2594	T4308	T4306	prep	in,reduced
R2595	T4309	T4310	det	the,mice
R2596	T4310	T4308	pobj	mice,in
R2597	T4311	T4312	nummod	12,month
R2598	T4312	T4314	npadvmod	month,old
R2599	T4313	T4312	punct	-,month
R26	T262	T263	nummod	68,kDa
R260	T511	T510	punct	-,old
R2600	T4314	T4310	amod	old,mice
R2601	T4315	T4314	punct	-,old
R2602	T4316	T4310	nmod	Sam68,mice
R2603	T4317	T4316	punct	+,Sam68
R2604	T4318	T4316	punct	/,Sam68
R2605	T4319	T4316	punct	+,Sam68
R2606	T4320	T4321	punct	(,5E
R2607	T4321	T4306	parataxis	5E,reduced
R2608	T4322	T4321	compound	Figure,5E
R2609	T4323	T4321	cc	and,5E
R261	T512	T506	pobj	mice,from
R2610	T4324	T4321	conj	5F,5E
R2611	T4325	T4321	punct	),5E
R2612	T4326	T4306	cc	and,reduced
R2613	T4327	T4306	conj	remained,reduced
R2614	T4328	T4327	acomp	unchanged,remained
R2615	T4329	T4327	prep	in,remained
R2616	T4330	T4331	nummod	12,month
R2617	T4331	T4333	npadvmod	month,old
R2618	T4332	T4331	punct	-,month
R2619	T4333	T4335	amod	old,mice
R262	T513	T512	nmod	Sam68,mice
R2620	T4334	T4333	punct	-,old
R2621	T4335	T4329	pobj	mice,in
R2622	T4336	T4335	nmod	Sam68,mice
R2623	T4337	T4336	punct	−,Sam68
R2624	T4338	T4336	punct	/,Sam68
R2625	T4339	T4336	punct	−,Sam68
R2626	T4340	T4341	punct	(,5G
R2627	T4341	T4327	parataxis	5G,remained
R2628	T4342	T4341	compound	Figure,5G
R2629	T4343	T4341	cc	and,5G
R263	T514	T513	punct	−,Sam68
R2630	T4344	T4341	conj	5H,5G
R2631	T4345	T4341	punct	),5G
R2632	T4346	T4306	punct	.,reduced
R2633	T4348	T4349	det	The,reduction
R2634	T4349	T4350	nsubj	reduction,argued
R2635	T4351	T4349	prep	in,reduction
R2636	T4352	T4353	preconj	both,osteoblast
R2637	T4353	T4354	nmod	osteoblast,activity
R2638	T4354	T4351	pobj	activity,in
R2639	T4355	T4353	cc	and,osteoblast
R264	T515	T513	punct	/,Sam68
R2640	T4356	T4353	conj	osteoclast,osteoblast
R2641	T4357	T4349	prep	in,reduction
R2642	T4358	T4359	det	the,mice
R2643	T4359	T4357	pobj	mice,in
R2644	T4360	T4361	nummod	12,month
R2645	T4361	T4363	npadvmod	month,old
R2646	T4362	T4361	punct	-,month
R2647	T4363	T4359	amod	old,mice
R2648	T4364	T4363	punct	-,old
R2649	T4365	T4359	nmod	Sam68,mice
R265	T516	T513	punct	−,Sam68
R2650	T4366	T4365	punct	+,Sam68
R2651	T4367	T4365	punct	/,Sam68
R2652	T4368	T4365	punct	+,Sam68
R2653	T4369	T4350	prep	against,argued
R2654	T4370	T4371	nsubjpass	bone,lost
R2655	T4371	T4369	pcomp	lost,against
R2656	T4372	T4371	auxpass	being,lost
R2657	T4373	T4374	advmod	primarily,due
R2658	T4374	T4371	prep	due,lost
R2659	T4375	T4374	pcomp	to,due
R266	T517	T518	amod	few,adipocytes
R2660	T4376	T4377	det	a,increase
R2661	T4377	T4374	pobj	increase,due
R2662	T4378	T4377	amod	relative,increase
R2663	T4379	T4377	prep	in,increase
R2664	T4380	T4379	pobj	osteoclast,in
R2665	T4381	T4380	prep	over,osteoclast
R2666	T4382	T4381	pobj	osteoblast,over
R2667	T4383	T4380	appos	activity,osteoclast
R2668	T4384	T4371	punct	", ",lost
R2669	T4385	T4386	mark	as,seen
R267	T518	T502	dobj	adipocytes,had
R2670	T4386	T4371	advcl	seen,lost
R2671	T4387	T4386	prep	in,seen
R2672	T4388	T4389	amod	high,turnover
R2673	T4389	T4390	compound	turnover,disease
R2674	T4390	T4387	pobj	disease,in
R2675	T4391	T4392	punct	[,49
R2676	T4392	T4350	parataxis	49,argued
R2677	T4393	T4392	punct	],49
R2678	T4394	T4350	punct	.,argued
R2679	T4396	T4397	amod	Histomorphometric,analyses
R268	T519	T518	compound	marrow,adipocytes
R2680	T4397	T4398	nsubj	analyses,corroborated
R2681	T4399	T4400	punct	[,50
R2682	T4400	T4397	parataxis	50,analyses
R2683	T4401	T4400	punct	],50
R2684	T4402	T4397	prep	of,analyses
R2685	T4403	T4404	det	the,bones
R2686	T4404	T4402	pobj	bones,of
R2687	T4405	T4404	amod	long,bones
R2688	T4406	T4407	punct	(,Table
R2689	T4407	T4397	parataxis	Table,analyses
R269	T520	T502	prep	compared,had
R2690	T4408	T4407	nummod	3,Table
R2691	T4409	T4407	punct	),Table
R2692	T4410	T4411	det	the,evidence
R2693	T4411	T4398	dobj	evidence,corroborated
R2694	T4412	T4411	amod	radiologic,evidence
R2695	T4413	T4411	prep	of,evidence
R2696	T4414	T4415	npadvmod	age,related
R2697	T4415	T4417	amod	related,loss
R2698	T4416	T4415	punct	-,related
R2699	T4417	T4413	pobj	loss,of
R27	T263	T261	pobj	kDa,of
R270	T521	T520	prep	with,compared
R2700	T4418	T4417	compound	bone,loss
R2701	T4419	T4398	punct	.,corroborated
R2702	T4421	T4422	compound	Bone,volume
R2703	T4422	T4423	nsubjpass	volume,reduced
R2704	T4424	T4422	punct	(,volume
R2705	T4425	T4426	compound	BV,TV
R2706	T4426	T4422	appos	TV,volume
R2707	T4427	T4426	punct	/,TV
R2708	T4428	T4422	punct	),volume
R2709	T4429	T4422	punct	", ",volume
R271	T522	T523	poss	their,controls
R2710	T4430	T4431	advmod	newly,formed
R2711	T4431	T4432	amod	formed,osteoid
R2712	T4432	T4422	conj	osteoid,volume
R2713	T4433	T4432	punct	(,osteoid
R2714	T4434	T4435	compound	OV,TV
R2715	T4435	T4432	appos	TV,osteoid
R2716	T4436	T4435	punct	/,TV
R2717	T4437	T4432	punct	),osteoid
R2718	T4438	T4432	punct	", ",osteoid
R2719	T4439	T4432	cc	and,osteoid
R272	T523	T521	pobj	controls,with
R2720	T4440	T4441	compound	mineral,rate
R2721	T4441	T4432	conj	rate,osteoid
R2722	T4442	T4441	compound	apposition,rate
R2723	T4443	T4441	punct	(,rate
R2724	T4444	T4441	appos	MAR,rate
R2725	T4445	T4423	punct	),reduced
R2726	T4446	T4423	auxpass	were,reduced
R2727	T4447	T4423	dep	all,reduced
R2728	T4448	T4423	advmod	significantly,reduced
R2729	T4449	T4423	prep	in,reduced
R273	T524	T525	npadvmod	age,matched
R2730	T4450	T4451	nummod	12,month
R2731	T4451	T4453	npadvmod	month,old
R2732	T4452	T4451	punct	-,month
R2733	T4453	T4455	amod	old,mice
R2734	T4454	T4453	punct	-,old
R2735	T4455	T4449	pobj	mice,in
R2736	T4456	T4455	nmod	Sam68,mice
R2737	T4457	T4456	punct	+,Sam68
R2738	T4458	T4456	punct	/,Sam68
R2739	T4459	T4456	punct	+,Sam68
R274	T525	T523	amod	matched,controls
R2740	T4460	T4423	prep	compared,reduced
R2741	T4461	T4460	prep	with,compared
R2742	T4462	T4463	nummod	4,month
R2743	T4463	T4465	npadvmod	month,old
R2744	T4464	T4463	punct	-,month
R2745	T4465	T4467	amod	old,mice
R2746	T4466	T4465	punct	-,old
R2747	T4467	T4461	pobj	mice,with
R2748	T4468	T4467	nmod	Sam68,mice
R2749	T4469	T4468	punct	+,Sam68
R275	T526	T525	punct	-,matched
R2750	T4470	T4468	punct	/,Sam68
R2751	T4471	T4468	punct	+,Sam68
R2752	T4472	T4423	punct	.,reduced
R2753	T4474	T4475	det	These,reductions
R2754	T4475	T4476	nsubjpass	reductions,associated
R2755	T4477	T4476	auxpass	were,associated
R2756	T4478	T4476	prep	with,associated
R2757	T4479	T4480	det	a,increase
R2758	T4480	T4478	pobj	increase,with
R2759	T4481	T4480	amod	significant,increase
R276	T527	T528	amod	wild,type
R2760	T4482	T4480	prep	in,increase
R2761	T4483	T4484	compound	marrow,fat
R2762	T4484	T4482	pobj	fat,in
R2763	T4485	T4486	punct	(,TV
R2764	T4486	T4480	parataxis	TV,increase
R2765	T4487	T4486	compound	FV,TV
R2766	T4488	T4486	punct	/,TV
R2767	T4489	T4486	punct	),TV
R2768	T4490	T4480	cc	and,increase
R2769	T4491	T4492	amod	decreased,numbers
R277	T528	T523	compound	type,controls
R2770	T4492	T4480	conj	numbers,increase
R2771	T4493	T4492	prep	of,numbers
R2772	T4494	T4493	pobj	osteoblasts,of
R2773	T4495	T4496	punct	(,TV
R2774	T4496	T4494	parataxis	TV,osteoblasts
R2775	T4497	T4496	compound	nOB,TV
R2776	T4498	T4496	punct	/,TV
R2777	T4499	T4496	punct	),TV
R2778	T4500	T4494	cc	and,osteoblasts
R2779	T4501	T4494	conj	osteoclasts,osteoblasts
R278	T529	T528	punct	-,type
R2780	T4502	T4503	punct	(,TV
R2781	T4503	T4501	parataxis	TV,osteoclasts
R2782	T4504	T4503	compound	nOC,TV
R2783	T4505	T4503	punct	/,TV
R2784	T4506	T4503	punct	),TV
R2785	T4507	T4492	prep	per,numbers
R2786	T4508	T4509	compound	tissue,volume
R2787	T4509	T4507	pobj	volume,per
R2788	T4510	T4511	punct	(,Table
R2789	T4511	T4476	parataxis	Table,associated
R279	T530	T523	compound	littermate,controls
R2790	T4512	T4511	nummod	3,Table
R2791	T4513	T4511	punct	),Table
R2792	T4514	T4476	punct	.,associated
R2793	T4516	T4517	det	These,results
R2794	T4517	T4518	nsubj	results,were
R2795	T4519	T4518	prep	in,were
R2796	T4520	T4519	pobj	contrast,in
R2797	T4521	T4520	prep	to,contrast
R2798	T4522	T4521	pobj	those,to
R2799	T4523	T4522	prep	of,those
R28	T264	T265	punct	(,Sam68
R280	T531	T523	punct	", ",controls
R2800	T4524	T4525	det	the,mice
R2801	T4525	T4523	pobj	mice,of
R2802	T4526	T4527	nummod	12,month
R2803	T4527	T4529	npadvmod	month,old
R2804	T4528	T4527	punct	-,month
R2805	T4529	T4525	amod	old,mice
R2806	T4530	T4529	punct	-,old
R2807	T4531	T4525	nmod	Sam68,mice
R2808	T4532	T4531	punct	−,Sam68
R2809	T4533	T4531	punct	/,Sam68
R281	T532	T533	dep	which,exhibited
R2810	T4534	T4531	punct	−,Sam68
R2811	T4535	T4536	prep	in,resembled
R2812	T4536	T4522	relcl	resembled,those
R2813	T4537	T4535	pobj	which,in
R2814	T4538	T4539	det	all,parameters
R2815	T4539	T4536	nsubj	parameters,resembled
R2816	T4540	T4539	amod	histomorphometric,parameters
R2817	T4541	T4539	punct	", ",parameters
R2818	T4542	T4539	prep	including,parameters
R2819	T4543	T4544	compound	marrow,fat
R282	T533	T523	relcl	exhibited,controls
R2820	T4544	T4542	pobj	fat,including
R2821	T4545	T4536	punct	", ",resembled
R2822	T4546	T4536	dobj	those,resembled
R2823	T4547	T4546	prep	of,those
R2824	T4548	T4549	amod	young,mice
R2825	T4549	T4547	pobj	mice,of
R2826	T4550	T4549	prep	of,mice
R2827	T4551	T4552	preconj	either,genotype
R2828	T4552	T4550	pobj	genotype,of
R2829	T4553	T4554	punct	(,Table
R283	T534	T535	amod	fatty,marrow
R2830	T4554	T4536	parataxis	Table,resembled
R2831	T4555	T4554	nummod	3,Table
R2832	T4556	T4554	punct	),Table
R2833	T4557	T4518	punct	.,were
R2834	T4559	T4560	advmod	When,expressed
R2835	T4560	T4563	advcl	expressed,were
R2836	T4561	T4560	nsubjpass	cells,expressed
R2837	T4562	T4560	auxpass	were,expressed
R2838	T4564	T4560	prep	as,expressed
R2839	T4565	T4566	det	a,function
R284	T535	T533	dobj	marrow,exhibited
R2840	T4566	T4564	pobj	function,as
R2841	T4567	T4566	prep	of,function
R2842	T4568	T4569	det	the,perimeter
R2843	T4569	T4567	pobj	perimeter,of
R2844	T4570	T4569	compound	bone,perimeter
R2845	T4571	T4572	punct	(,BP
R2846	T4572	T4560	parataxis	BP,expressed
R2847	T4573	T4572	compound	nOB,BP
R2848	T4574	T4572	punct	/,BP
R2849	T4575	T4572	punct	", ",BP
R285	T536	T535	compound	bone,marrow
R2850	T4576	T4577	compound	nOC,BP
R2851	T4577	T4572	appos	BP,BP
R2852	T4578	T4577	punct	/,BP
R2853	T4579	T4572	punct	),BP
R2854	T4580	T4563	punct	", ",were
R2855	T4581	T4563	expl	there,were
R2856	T4582	T4583	det	no,differences
R2857	T4583	T4563	attr	differences,were
R2858	T4584	T4583	amod	statistical,differences
R2859	T4585	T4563	cc	and,were
R286	T537	T495	punct	.,shown
R2860	T4586	T4587	det	the,ratio
R2861	T4587	T4588	nsubj	ratio,was
R2862	T4588	T4563	conj	was,were
R2863	T4589	T4587	prep	of,ratio
R2864	T4590	T4589	pobj	osteoblasts,of
R2865	T4591	T4590	prep	to,osteoblasts
R2866	T4592	T4591	pobj	osteoclasts,to
R2867	T4593	T4588	acomp	similar,was
R2868	T4594	T4588	prep	in,was
R2869	T4595	T4596	det	all,groups
R287	T539	T540	poss	Our,findings
R2870	T4596	T4594	pobj	groups,in
R2871	T4597	T4596	prep	of,groups
R2872	T4598	T4597	pobj	mice,of
R2873	T4599	T4600	punct	(,Table
R2874	T4600	T4588	parataxis	Table,was
R2875	T4601	T4600	nummod	3,Table
R2876	T4602	T4600	punct	),Table
R2877	T4603	T4563	punct	.,were
R2878	T4605	T4606	amod	Circulating,levels
R2879	T4606	T4607	nsubj	levels,showed
R288	T236	T237	nsubj	Ablation,Protects
R2880	T4608	T4606	prep	of,levels
R2881	T4609	T4610	compound	serum,telopeptide
R2882	T4610	T4608	pobj	telopeptide,of
R2883	T4611	T4610	compound	C,telopeptide
R2884	T4612	T4610	punct	-,telopeptide
R2885	T4613	T4614	punct	(,Diagnostics
R2886	T4614	T4610	parataxis	Diagnostics,telopeptide
R2887	T4615	T4614	dep	sCTX,Diagnostics
R2888	T4616	T4614	punct	;,Diagnostics
R2889	T4617	T4614	compound	Roche,Diagnostics
R289	T540	T541	nsubj	findings,identify
R2890	T4618	T4614	punct	", ",Diagnostics
R2891	T4619	T4614	npadvmod	Mannheim,Diagnostics
R2892	T4620	T4614	punct	", ",Diagnostics
R2893	T4621	T4614	npadvmod	Germany,Diagnostics
R2894	T4622	T4614	punct	),Diagnostics
R2895	T4623	T4610	cc	and,telopeptide
R2896	T4624	T4610	conj	ALP,telopeptide
R2897	T4625	T4626	amod	little,difference
R2898	T4626	T4607	dobj	difference,showed
R2899	T4627	T4607	prep	among,showed
R29	T265	T255	parataxis	Sam68,substrate
R290	T542	T543	amod	endogenous,Sam68
R2900	T4628	T4629	det	the,groups
R2901	T4629	T4627	pobj	groups,among
R2902	T4630	T4607	punct	", ",showed
R2903	T4631	T4607	prep	except,showed
R2904	T4632	T4631	prep	for,except
R2905	T4633	T4634	det	a,decrease
R2906	T4634	T4632	pobj	decrease,for
R2907	T4635	T4634	amod	small,decrease
R2908	T4636	T4634	prep	in,decrease
R2909	T4637	T4638	nummod	12,month
R291	T543	T541	dobj	Sam68,identify
R2910	T4638	T4640	npadvmod	month,old
R2911	T4639	T4638	punct	-,month
R2912	T4640	T4642	amod	old,mice
R2913	T4641	T4640	punct	-,old
R2914	T4642	T4636	pobj	mice,in
R2915	T4643	T4642	nmod	Sam68,mice
R2916	T4644	T4643	punct	−,Sam68
R2917	T4645	T4643	punct	/,Sam68
R2918	T4646	T4643	punct	−,Sam68
R2919	T4647	T4642	cc	and,mice
R292	T544	T541	prep	as,identify
R2920	T4648	T4649	punct	(,S3
R2921	T4649	T4642	conj	S3,mice
R2922	T4650	T4649	compound	Dataset,S3
R2923	T4651	T4607	punct	),showed
R2924	T4652	T4607	punct	.,showed
R2925	T4654	T4655	compound	Serum,estrogen
R2926	T4655	T4656	nsubjpass	estrogen,decreased
R2927	T4657	T4656	auxpass	was,decreased
R2928	T4658	T4656	advmod	significantly,decreased
R2929	T4659	T4656	prep	in,decreased
R293	T545	T546	det	a,regulator
R2930	T4660	T4661	det	all,mice
R2931	T4661	T4659	pobj	mice,in
R2932	T4662	T4663	nummod	12,month
R2933	T4663	T4665	npadvmod	month,old
R2934	T4664	T4663	punct	-,month
R2935	T4665	T4661	amod	old,mice
R2936	T4666	T4665	punct	-,old
R2937	T4667	T4656	advmod	regardless,decreased
R2938	T4668	T4667	prep	of,regardless
R2939	T4669	T4668	pobj	genotype,of
R294	T546	T544	pobj	regulator,as
R2940	T4670	T4656	prep	with,decreased
R2941	T4671	T4672	det	a,increase
R2942	T4672	T4670	pobj	increase,with
R2943	T4673	T4672	amod	reciprocal,increase
R2944	T4674	T4672	prep	in,increase
R2945	T4675	T4676	nmod	interleukin,levels
R2946	T4676	T4674	pobj	levels,in
R2947	T4677	T4675	punct	-,interleukin
R2948	T4678	T4675	nummod	6,interleukin
R2949	T4679	T4680	punct	(,S3
R295	T547	T546	amod	positive,regulator
R2950	T4680	T4656	parataxis	S3,decreased
R2951	T4681	T4680	compound	Dataset,S3
R2952	T4682	T4680	punct	),S3
R2953	T4683	T4656	punct	.,decreased
R2954	T4685	T4686	prep	Given,measured
R2955	T4687	T4688	det	the,involvement
R2956	T4688	T4685	pobj	involvement,Given
R2957	T4689	T4688	prep	of,involvement
R2958	T4690	T4689	pobj	leptin,of
R2959	T4691	T4688	prep	in,involvement
R296	T548	T546	prep	of,regulator
R2960	T4692	T4691	pcomp	regulating,in
R2961	T4693	T4694	nmod	body,mass
R2962	T4694	T4692	dobj	mass,regulating
R2963	T4695	T4693	cc	and,body
R2964	T4696	T4693	conj	bone,body
R2965	T4697	T4686	punct	", ",measured
R2966	T4698	T4699	compound	serum,leptin
R2967	T4699	T4686	nsubjpass	leptin,measured
R2968	T4700	T4686	auxpass	was,measured
R2969	T4701	T4686	prep	in,measured
R297	T549	T550	compound	adipocyte,differentiation
R2970	T4702	T4703	nmod	Sam68,mice
R2971	T4703	T4701	pobj	mice,in
R2972	T4704	T4702	punct	+,Sam68
R2973	T4705	T4702	punct	/,Sam68
R2974	T4706	T4702	punct	+,Sam68
R2975	T4707	T4702	cc	and,Sam68
R2976	T4708	T4702	conj	Sam68,Sam68
R2977	T4709	T4708	punct	−,Sam68
R2978	T4710	T4708	punct	/,Sam68
R2979	T4711	T4708	punct	−,Sam68
R298	T550	T548	pobj	differentiation,of
R2980	T4712	T4686	punct	.,measured
R2981	T4714	T4715	nummod	Twelve,month
R2982	T4715	T4717	npadvmod	month,old
R2983	T4716	T4715	punct	-,month
R2984	T4717	T4719	amod	old,mice
R2985	T4718	T4717	punct	-,old
R2986	T4719	T4724	nsubj	mice,had
R2987	T4720	T4719	nmod	Sam68,mice
R2988	T4721	T4720	punct	−,Sam68
R2989	T4722	T4720	punct	/,Sam68
R299	T551	T546	cc	and,regulator
R2990	T4723	T4720	punct	−,Sam68
R2991	T4725	T4726	advmod	significantly,lower
R2992	T4726	T4727	amod	lower,levels
R2993	T4727	T4724	dobj	levels,had
R2994	T4728	T4727	prep	than,levels
R2995	T4729	T4730	amod	young,mice
R2996	T4730	T4728	pobj	mice,than
R2997	T4731	T4729	cc	and,young
R2998	T4732	T4729	conj	old,young
R2999	T4733	T4730	nmod	Sam68,mice
R30	T266	T265	punct	),Sam68
R300	T552	T553	det	a,regulator
R3000	T4734	T4733	punct	+,Sam68
R3001	T4735	T4733	punct	/,Sam68
R3002	T4736	T4733	punct	+,Sam68
R3003	T4737	T4730	cc	and,mice
R3004	T4738	T4739	amod	young,mice
R3005	T4739	T4730	conj	mice,mice
R3006	T4740	T4739	nmod	Sam68,mice
R3007	T4741	T4740	punct	−,Sam68
R3008	T4742	T4740	punct	/,Sam68
R3009	T4743	T4740	punct	−,Sam68
R301	T553	T546	conj	regulator,regulator
R3010	T4744	T4745	punct	(,S3
R3011	T4745	T4724	parataxis	S3,had
R3012	T4746	T4745	compound	Dataset,S3
R3013	T4747	T4745	punct	),S3
R3014	T4748	T4724	punct	.,had
R3015	T4750	T4751	advcl	Taken,suggested
R3016	T4752	T4750	advmod	together,Taken
R3017	T4753	T4751	punct	", ",suggested
R3018	T4754	T4755	det	these,observations
R3019	T4755	T4751	nsubj	observations,suggested
R302	T554	T553	amod	negative,regulator
R3020	T4756	T4757	mark	that,was
R3021	T4757	T4751	ccomp	was,suggested
R3022	T4758	T4757	nsubj	preservation,was
R3023	T4759	T4758	prep	of,preservation
R3024	T4760	T4761	compound	bone,mass
R3025	T4761	T4759	pobj	mass,of
R3026	T4762	T4758	prep	in,preservation
R3027	T4763	T4764	det	the,mice
R3028	T4764	T4762	pobj	mice,in
R3029	T4765	T4764	nmod	Sam68,mice
R303	T555	T553	prep	of,regulator
R3030	T4766	T4765	punct	−,Sam68
R3031	T4767	T4765	punct	/,Sam68
R3032	T4768	T4765	punct	−,Sam68
R3033	T4769	T4757	neg	not,was
R3034	T4770	T4757	prep	due,was
R3035	T4771	T4770	advmod	primarily,due
R3036	T4772	T4770	pcomp	to,due
R3037	T4773	T4774	amod	altered,status
R3038	T4774	T4770	pobj	status,due
R3039	T4775	T4774	compound	estrogen,status
R304	T556	T557	compound	osteoblast,differentiation
R3040	T4776	T4757	cc	but,was
R3041	T4777	T4778	aux	could,influenced
R3042	T4778	T4757	conj	influenced,was
R3043	T4779	T4778	aux	have,influenced
R3044	T4780	T4778	auxpass	been,influenced
R3045	T4781	T4778	agent	by,influenced
R3046	T4782	T4781	pobj	differences,by
R3047	T4783	T4782	prep	in,differences
R3048	T4784	T4785	amod	circulating,levels
R3049	T4785	T4783	pobj	levels,in
R305	T557	T555	pobj	differentiation,of
R3050	T4786	T4785	compound	leptin,levels
R3051	T4787	T4751	punct	.,suggested
R3052	T4956	T4957	nmod	Osteoblast,Activity
R3053	T4957	T4963	nsubjpass	Activity,Altered
R3054	T4958	T4956	punct	", ",Osteoblast
R3055	T4959	T4956	cc	but,Osteoblast
R3056	T4960	T4959	neg	Not,but
R3057	T4961	T4956	conj	Osteoclast,Osteoblast
R3058	T4962	T4957	punct	", ",Activity
R3059	T4964	T4963	auxpass	Is,Altered
R306	T558	T541	punct	", ",identify
R3060	T4965	T4963	prep	in,Altered
R3061	T4966	T4967	nmod	Sam68,Mice
R3062	T4967	T4965	pobj	Mice,in
R3063	T4968	T4966	punct	−,Sam68
R3064	T4969	T4966	punct	/,Sam68
R3065	T4970	T4966	punct	−,Sam68
R3066	T4971	T4972	advmod	Ex,Vivo
R3067	T4972	T4963	advmod	Vivo,Altered
R3068	T4974	T4975	nsubj	Maintenance,is
R3069	T4976	T4974	prep	of,Maintenance
R307	T559	T560	dep	which,is
R3070	T4977	T4978	compound	bone,mass
R3071	T4978	T4976	pobj	mass,of
R3072	T4979	T4974	prep	during,Maintenance
R3073	T4980	T4981	det	the,cycle
R3074	T4981	T4979	pobj	cycle,during
R3075	T4982	T4981	amod	adult,cycle
R3076	T4983	T4981	compound	remodeling,cycle
R3077	T4984	T4975	acomp	dependent,is
R3078	T4985	T4984	prep	on,dependent
R3079	T4986	T4987	det	the,coupling
R308	T560	T541	advcl	is,identify
R3080	T4987	T4985	pobj	coupling,on
R3081	T4988	T4987	prep	of,coupling
R3082	T4989	T4988	pobj	osteoblast,of
R3083	T4990	T4987	prep	to,coupling
R3084	T4991	T4990	pobj	osteoclast,to
R3085	T4992	T4987	appos	activity,coupling
R3086	T4993	T4975	punct	", ",is
R3087	T4994	T4995	amod	such,is
R3088	T4995	T4975	advcl	is,is
R3089	T4996	T4995	mark	that,is
R309	T561	T560	acomp	consistent,is
R3090	T4997	T4995	expl	there,is
R3091	T4998	T4999	det	no,gain
R3092	T4999	T4995	attr	gain,is
R3093	T5000	T4999	amod	net,gain
R3094	T5001	T4999	cc	or,gain
R3095	T5002	T4999	conj	loss,gain
R3096	T5003	T4999	prep	of,gain
R3097	T5004	T5003	pobj	bone,of
R3098	T5005	T5006	punct	[,49
R3099	T5006	T4975	parataxis	49,is
R31	T268	T269	det	a,protein
R310	T562	T561	prep	with,consistent
R3100	T5007	T5006	punct	],49
R3101	T5008	T4975	punct	.,is
R3102	T5010	T5011	aux	To,explore
R3103	T5011	T5013	advcl	explore,examined
R3104	T5012	T5011	advmod	further,explore
R3105	T5014	T5015	det	the,advantage
R3106	T5015	T5011	dobj	advantage,explore
R3107	T5016	T5017	npadvmod	age,related
R3108	T5017	T5015	amod	related,advantage
R3109	T5018	T5017	punct	-,related
R311	T563	T564	nsubj	Sam68,being
R3110	T5019	T5015	prep	of,advantage
R3111	T5020	T5021	nmod	Sam68,mice
R3112	T5021	T5019	pobj	mice,of
R3113	T5022	T5020	punct	−,Sam68
R3114	T5023	T5020	punct	/,Sam68
R3115	T5024	T5020	punct	−,Sam68
R3116	T5025	T5011	prep	with,explore
R3117	T5026	T5025	pobj	respect,with
R3118	T5027	T5026	prep	to,respect
R3119	T5028	T5029	compound	bone,preservation
R312	T564	T562	pcomp	being,with
R3120	T5029	T5027	pobj	preservation,to
R3121	T5030	T5013	punct	", ",examined
R3122	T5031	T5013	nsubj	we,examined
R3123	T5032	T5033	det	the,activity
R3124	T5033	T5013	dobj	activity,examined
R3125	T5034	T5033	amod	functional,activity
R3126	T5035	T5033	prep	of,activity
R3127	T5036	T5035	pobj	Sam68,of
R3128	T5037	T5036	punct	−,Sam68
R3129	T5038	T5036	punct	/,Sam68
R313	T565	T566	det	a,modulator
R3130	T5039	T5036	punct	−,Sam68
R3131	T5040	T5036	appos	osteoblasts,Sam68
R3132	T5041	T5040	cc	and,osteoblasts
R3133	T5042	T5040	conj	osteoclasts,osteoblasts
R3134	T5043	T5044	advmod	ex,vivo
R3135	T5044	T5013	advmod	vivo,examined
R3136	T5045	T5046	punct	(,6A
R3137	T5046	T5013	parataxis	6A,examined
R3138	T5047	T5046	compound	Figure,6A
R3139	T5048	T5046	cc	and,6A
R314	T566	T564	attr	modulator,being
R3140	T5049	T5046	conj	6B,6A
R3141	T5050	T5046	punct	),6A
R3142	T5051	T5013	punct	.,examined
R3143	T5053	T5054	nsubj	Cultures,demonstrated
R3144	T5055	T5053	prep	of,Cultures
R3145	T5056	T5057	nmod	bone,marrow
R3146	T5057	T5058	nmod	marrow,cells
R3147	T5058	T5055	pobj	cells,of
R3148	T5059	T5058	amod	stromal,cells
R3149	T5060	T5053	acl	harvested,Cultures
R315	T567	T566	prep	of,modulator
R3150	T5061	T5060	prep	from,harvested
R3151	T5062	T5063	nummod	4,week
R3152	T5063	T5065	npadvmod	week,old
R3153	T5064	T5063	punct	-,week
R3154	T5065	T5067	amod	old,mice
R3155	T5066	T5065	punct	-,old
R3156	T5067	T5061	pobj	mice,from
R3157	T5068	T5067	nmod	Sam68,mice
R3158	T5069	T5068	punct	−,Sam68
R3159	T5070	T5068	punct	/,Sam68
R316	T568	T569	nmod	bone,marrow
R3160	T5071	T5068	punct	−,Sam68
R3161	T5072	T5060	cc	and,harvested
R3162	T5073	T5060	conj	maintained,harvested
R3163	T5074	T5073	prep	for,maintained
R3164	T5075	T5076	nummod	18,days
R3165	T5076	T5074	pobj	days,for
R3166	T5077	T5073	prep	in,maintained
R3167	T5078	T5079	compound	osteoblast,differentiation
R3168	T5079	T5080	compound	differentiation,medium
R3169	T5080	T5077	pobj	medium,in
R317	T569	T570	nmod	marrow,differentiation
R3170	T5081	T5082	advmod	more,intense
R3171	T5082	T5083	amod	intense,staining
R3172	T5083	T5054	dobj	staining,demonstrated
R3173	T5084	T5083	prep	for,staining
R3174	T5085	T5084	pobj	ALP,for
R3175	T5086	T5054	prep	at,demonstrated
R3176	T5087	T5088	nummod	6,days
R3177	T5088	T5086	pobj	days,at
R3178	T5089	T5090	punct	(,data
R3179	T5090	T5088	meta	data,days
R318	T570	T567	pobj	differentiation,of
R3180	T5091	T5090	amod	unpublished,data
R3181	T5092	T5090	punct	),data
R3182	T5093	T5088	cc	and,days
R3183	T5094	T5095	nummod	18,days
R3184	T5095	T5088	conj	days,days
R3185	T5096	T5097	punct	(,6A
R3186	T5097	T5095	parataxis	6A,days
R3187	T5098	T5097	compound	Figure,6A
R3188	T5099	T5097	dep	),6A
R3189	T5100	T5054	dep	", ",demonstrated
R319	T571	T572	amod	mesenchymal,cell
R3190	T5101	T5054	cc	and,demonstrated
R3191	T5102	T5103	amod	more,nodules
R3192	T5103	T5054	conj	nodules,demonstrated
R3193	T5104	T5103	amod	mineralized,nodules
R3194	T5105	T5103	prep	at,nodules
R3195	T5106	T5107	nummod	18,days
R3196	T5107	T5105	pobj	days,at
R3197	T5108	T5054	dep	", ",demonstrated
R3198	T5109	T5054	prep	than,demonstrated
R3199	T5110	T5111	det	the,mice
R32	T269	T257	attr	protein,is
R320	T572	T570	compound	cell,differentiation
R3200	T5111	T5109	pobj	mice,than
R3201	T5112	T5111	nmod	Sam68,mice
R3202	T5113	T5112	punct	+,Sam68
R3203	T5114	T5112	punct	/,Sam68
R3204	T5115	T5112	punct	+,Sam68
R3205	T5116	T5054	dep	", ",demonstrated
R3206	T5117	T5118	advmod	even,observed
R3207	T5118	T5054	advcl	observed,demonstrated
R3208	T5119	T5118	mark	though,observed
R3209	T5120	T5121	amod	similar,amounts
R321	T573	T570	punct	", ",differentiation
R3210	T5121	T5118	nsubjpass	amounts,observed
R3211	T5122	T5121	prep	of,amounts
R3212	T5123	T5124	nmod	fibroblast,units
R3213	T5124	T5122	pobj	units,of
R3214	T5125	T5126	npadvmod	colony,forming
R3215	T5126	T5124	amod	forming,units
R3216	T5127	T5126	punct	-,forming
R3217	T5128	T5124	punct	(,units
R3218	T5129	T5130	compound	CFU,F
R3219	T5130	T5124	appos	F,units
R322	T574	T570	cc	and,differentiation
R3220	T5131	T5130	punct	-,F
R3221	T5132	T5118	punct	),observed
R3222	T5133	T5118	auxpass	were,observed
R3223	T5134	T5135	punct	(,6A
R3224	T5135	T5118	parataxis	6A,observed
R3225	T5136	T5135	compound	Figure,6A
R3226	T5137	T5135	cc	and,6A
R3227	T5138	T5139	amod	unpublished,data
R3228	T5139	T5135	conj	data,6A
R3229	T5140	T5135	punct	),6A
R323	T575	T576	advmod	hence,metabolism
R3230	T5141	T5054	punct	.,demonstrated
R3231	T5143	T5144	compound	RT,PCR
R3232	T5144	T5146	compound	PCR,analysis
R3233	T5145	T5144	punct	-,PCR
R3234	T5146	T5147	nsubj	analysis,revealed
R3235	T5148	T5146	prep	of,analysis
R3236	T5149	T5150	amod	molecular,markers
R3237	T5150	T5148	pobj	markers,of
R3238	T5151	T5150	prep	of,markers
R3239	T5152	T5153	compound	osteoblast,differentiation
R324	T576	T570	conj	metabolism,differentiation
R3240	T5153	T5151	pobj	differentiation,of
R3241	T5154	T5155	punct	(,S2
R3242	T5155	T5146	parataxis	S2,analysis
R3243	T5156	T5155	compound	Dataset,S2
R3244	T5157	T5155	punct	),S2
R3245	T5158	T5159	amod	similar,increases
R3246	T5159	T5147	dobj	increases,revealed
R3247	T5160	T5147	prep	over,revealed
R3248	T5161	T5160	pobj	time,over
R3249	T5162	T5147	prep	in,revealed
R325	T577	T576	compound	bone,metabolism
R3250	T5163	T5162	pobj	RNA,in
R3251	T5164	T5163	prep	from,RNA
R3252	T5165	T5166	nmod	Sam68,mice
R3253	T5166	T5164	pobj	mice,from
R3254	T5167	T5165	punct	+,Sam68
R3255	T5168	T5165	punct	/,Sam68
R3256	T5169	T5165	punct	+,Sam68
R3257	T5170	T5165	cc	and,Sam68
R3258	T5171	T5165	conj	Sam68,Sam68
R3259	T5172	T5171	punct	−,Sam68
R326	T578	T564	punct	", ",being
R3260	T5173	T5171	punct	/,Sam68
R3261	T5174	T5171	punct	−,Sam68
R3262	T5175	T5147	punct	", ",revealed
R3263	T5176	T5177	mark	although,appeared
R3264	T5177	T5147	advcl	appeared,revealed
R3265	T5178	T5179	nmod	type,collagen
R3266	T5179	T5177	nsubj	collagen,appeared
R3267	T5180	T5178	nummod	I,type
R3268	T5181	T5182	aux	to,regulated
R3269	T5182	T5177	xcomp	regulated,appeared
R327	T579	T564	prep	in,being
R3270	T5183	T5182	auxpass	be,regulated
R3271	T5184	T5182	advmod	up,regulated
R3272	T5185	T5182	punct	-,regulated
R3273	T5186	T5182	prep	at,regulated
R3274	T5187	T5188	det	both,timepoints
R3275	T5188	T5186	pobj	timepoints,at
R3276	T5189	T5182	prep	in,regulated
R3277	T5190	T5191	det	the,mice
R3278	T5191	T5189	pobj	mice,in
R3279	T5192	T5191	nmod	Sam68,mice
R328	T580	T581	amod	aged,mice
R3280	T5193	T5192	punct	−,Sam68
R3281	T5194	T5192	punct	/,Sam68
R3282	T5195	T5192	punct	−,Sam68
R3283	T5196	T5147	punct	.,revealed
R3284	T5198	T5199	amod	Short,term
R3285	T5199	T5201	compound	term,cultures
R3286	T5200	T5199	punct	-,term
R3287	T5201	T5202	nsubj	cultures,stained
R3288	T5203	T5201	prep	of,cultures
R3289	T5204	T5205	amod	mature,osteoclasts
R329	T581	T579	pobj	mice,in
R3290	T5205	T5203	pobj	osteoclasts,of
R3291	T5206	T5205	acl	released,osteoclasts
R3292	T5207	T5206	prep	from,released
R3293	T5208	T5209	det	the,bones
R3294	T5209	T5207	pobj	bones,from
R3295	T5210	T5209	amod	crushed,bones
R3296	T5211	T5209	amod	long,bones
R3297	T5212	T5209	prep	of,bones
R3298	T5213	T5214	nmod	Sam68,mice
R3299	T5214	T5212	pobj	mice,of
R33	T270	T271	compound	KH,type
R330	T582	T541	punct	.,identify
R3300	T5215	T5213	punct	+,Sam68
R3301	T5216	T5213	punct	/,Sam68
R3302	T5217	T5213	punct	+,Sam68
R3303	T5218	T5213	cc	and,Sam68
R3304	T5219	T5213	conj	Sam68,Sam68
R3305	T5220	T5219	punct	−,Sam68
R3306	T5221	T5219	punct	/,Sam68
R3307	T5222	T5219	punct	−,Sam68
R3308	T5223	T5224	advmod	equally,well
R3309	T5224	T5202	advmod	well,stained
R3310	T5225	T5202	prep	for,stained
R3311	T5226	T5225	pobj	TRAP,for
R3312	T5227	T5202	cc	and,stained
R3313	T5228	T5202	conj	excavated,stained
R3314	T5229	T5230	advmod	approximately,equal
R3315	T5230	T5231	amod	equal,numbers
R3316	T5231	T5228	dobj	numbers,excavated
R3317	T5232	T5231	prep	of,numbers
R3318	T5233	T5232	pobj	pits,of
R3319	T5234	T5233	prep	of,pits
R3320	T5235	T5236	amod	equal,size
R3321	T5236	T5234	pobj	size,of
R3322	T5237	T5228	prep	in,excavated
R3323	T5238	T5239	compound	dentin,slices
R3324	T5239	T5237	pobj	slices,in
R3325	T5240	T5228	punct	", ",excavated
R3326	T5241	T5242	mark	as,visualized
R3327	T5242	T5228	advcl	visualized,excavated
R3328	T5243	T5242	prep	by,visualized
R3329	T5244	T5243	pcomp	scanning,by
R3330	T5245	T5246	compound	electron,microscopy
R3331	T5246	T5244	dobj	microscopy,scanning
R3332	T5247	T5248	punct	(,6B
R3333	T5248	T5228	parataxis	6B,excavated
R3334	T5249	T5248	compound	Figure,6B
R3335	T5250	T5248	punct	),6B
R3336	T5251	T5202	punct	.,stained
R3337	T5253	T5254	det	These,findings
R3338	T5254	T5255	nsubj	findings,suggest
R3339	T5256	T5257	mark	that,be
R3340	T5257	T5255	ccomp	be,suggest
R3341	T5258	T5259	det	the,osteoclasts
R3342	T5259	T5257	nsubj	osteoclasts,be
R3343	T5260	T5257	aux	may,be
R3344	T5261	T5257	neg	not,be
R3345	T5262	T5263	det	the,defect
R3346	T5263	T5257	attr	defect,be
R3347	T5264	T5263	amod	primary,defect
R3348	T5265	T5263	prep	in,defect
R3349	T5266	T5267	nmod	Sam68,mice
R3350	T5267	T5265	pobj	mice,in
R3351	T5268	T5266	punct	−,Sam68
R3352	T5269	T5266	punct	/,Sam68
R3353	T5270	T5266	punct	−,Sam68
R3354	T5271	T5257	punct	", ",be
R3355	T5272	T5273	mark	as,are
R3356	T5273	T5257	advcl	are,be
R3357	T5274	T5273	nsubj	they,are
R3358	T5275	T5273	prep	in,are
R3359	T5276	T5277	nmod	Src,mice
R3360	T5277	T5275	pobj	mice,in
R3361	T5278	T5277	amod	null,mice
R3362	T5279	T5280	punct	[,48
R3363	T5280	T5273	parataxis	48,are
R3364	T5281	T5280	punct	],48
R3365	T5282	T5255	punct	.,suggest
R3377	T5689	T5690	det	The,Absence
R3378	T5690	T5691	nsubj	Absence,Prevents
R3379	T5692	T5690	prep	of,Absence
R3380	T5693	T5692	pobj	Sam68,of
R3381	T5694	T5695	compound	Adipocyte,Differentiation
R3382	T5695	T5691	dobj	Differentiation,Prevents
R3383	T5696	T5691	cc	and,Prevents
R3384	T5697	T5691	conj	Promotes,Prevents
R3385	T5698	T5699	compound	Osteoblast,Differentiation
R3386	T5699	T5697	dobj	Differentiation,Promotes
R3387	T5701	T5702	nsubjpass	It,recognized
R3388	T5703	T5702	auxpass	is,recognized
R3389	T5704	T5702	advmod	well,recognized
R3390	T5705	T5706	mark	that,give
R3391	T5706	T5702	advcl	give,recognized
R3392	T5707	T5708	nmod	bone,marrow
R3393	T5708	T5709	nmod	marrow,cells
R3394	T5709	T5706	nsubj	cells,give
R3395	T5710	T5709	amod	stromal,cells
R3396	T5711	T5706	dobj	rise,give
R3397	T5712	T5706	prep	to,give
R3398	T5713	T5714	det	both,osteoblasts
R3399	T5714	T5712	pobj	osteoblasts,to
R34	T271	T269	compound	type,protein
R340	T1057	T1058	prep	During,favored
R3400	T5715	T5714	cc	and,osteoblasts
R3401	T5716	T5714	conj	adipocytes,osteoblasts
R3402	T5717	T5706	cc	and,give
R3403	T5718	T5719	mark	that,accompanied
R3404	T5719	T5706	conj	accompanied,give
R3405	T5720	T5721	npadvmod	age,related
R3406	T5721	T5723	amod	related,loss
R3407	T5722	T5721	punct	-,related
R3408	T5723	T5719	nsubjpass	loss,accompanied
R3409	T5724	T5723	compound	bone,loss
R341	T1059	T1060	amod	skeletal,development
R3410	T5725	T5719	auxpass	is,accompanied
R3411	T5726	T5719	agent	by,accompanied
R3412	T5727	T5728	det	an,increase
R3413	T5728	T5726	pobj	increase,by
R3414	T5729	T5728	prep	in,increase
R3415	T5730	T5729	pobj	differentiation,in
R3416	T5731	T5719	prep	down,accompanied
R3417	T5732	T5733	det	the,lineage
R3418	T5733	T5731	pobj	lineage,down
R3419	T5734	T5733	compound	adipocyte,lineage
R342	T1060	T1057	pobj	development,During
R3420	T5735	T5736	punct	[,22
R3421	T5736	T5719	parataxis	22,accompanied
R3422	T5737	T5736	punct	],22
R3423	T5738	T5702	punct	.,recognized
R3424	T5740	T5741	advmod	Therefore,influence
R3425	T5742	T5741	punct	", ",influence
R3426	T5743	T5744	det	the,loss
R3427	T5744	T5741	nsubj	loss,influence
R3428	T5745	T5744	prep	of,loss
R3429	T5746	T5745	pobj	Sam68,of
R343	T1061	T1058	punct	", ",favored
R3430	T5747	T5741	aux	could,influence
R3431	T5748	T5749	det	the,cells
R3432	T5749	T5741	dobj	cells,influence
R3433	T5750	T5751	nmod	bone,marrow
R3434	T5751	T5749	nmod	marrow,cells
R3435	T5752	T5749	amod	stromal,cells
R3436	T5753	T5754	aux	to,differentiate
R3437	T5754	T5741	advcl	differentiate,influence
R3438	T5755	T5754	prep	along,differentiate
R3439	T5756	T5757	det	the,osteogenic
R344	T1062	T1063	det	the,activity
R3440	T5757	T5755	pobj	osteogenic,along
R3441	T5758	T5757	cc	versus,osteogenic
R3442	T5759	T5760	det	the,adipogenic
R3443	T5760	T5761	nmod	adipogenic,pathway
R3444	T5761	T5757	conj	pathway,osteogenic
R3445	T5762	T5741	punct	.,influence
R3446	T5764	T5765	advmod	Alternatively,regulate
R3447	T5766	T5765	punct	", ",regulate
R3448	T5767	T5768	det	the,loss
R3449	T5768	T5765	nsubj	loss,regulate
R345	T1063	T1058	nsubjpass	activity,favored
R3450	T5769	T5768	prep	of,loss
R3451	T5770	T5769	pobj	Sam68,of
R3452	T5771	T5765	aux	could,regulate
R3453	T5772	T5765	advmod	indirectly,regulate
R3454	T5773	T5774	compound	bone,mass
R3455	T5774	T5765	dobj	mass,regulate
R3456	T5775	T5765	prep	as,regulate
R3457	T5776	T5777	det	a,disturbance
R3458	T5777	T5775	pobj	disturbance,as
R3459	T5778	T5777	amod	general,disturbance
R346	T1064	T1063	amod	anabolic,activity
R3460	T5779	T5777	prep	of,disturbance
R3461	T5780	T5781	amod	neuroendocrine,control
R3462	T5781	T5779	pobj	control,of
R3463	T5782	T5783	mark	as,shown
R3464	T5783	T5765	advcl	shown,regulate
R3465	T5784	T5783	auxpass	was,shown
R3466	T5785	T5786	advmod	when,shown
R3467	T5786	T5783	advcl	shown,shown
R3468	T5787	T5786	nsubjpass	leptin,shown
R3469	T5788	T5787	cc	and,leptin
R347	T1065	T1063	prep	of,activity
R3470	T5789	T5790	det	the,axis
R3471	T5790	T5787	conj	axis,leptin
R3472	T5791	T5790	amod	sympathetic,axis
R3473	T5792	T5793	amod	nervous,system
R3474	T5793	T5790	compound	system,axis
R3475	T5794	T5786	auxpass	were,shown
R3476	T5795	T5796	aux	to,regulate
R3477	T5796	T5786	xcomp	regulate,shown
R3478	T5797	T5796	advmod	negatively,regulate
R3479	T5798	T5799	compound	bone,mass
R348	T1066	T1065	pobj	osteoblasts,of
R3480	T5799	T5796	dobj	mass,regulate
R3481	T5800	T5801	punct	[,8
R3482	T5801	T5765	parataxis	8,regulate
R3483	T5802	T5801	punct	],8
R3484	T5803	T5765	punct	.,regulate
R3485	T5805	T5806	aux	To,confirm
R3486	T5806	T5807	advcl	confirm,isolated
R3487	T5808	T5809	det	a,role
R3488	T5809	T5806	dobj	role,confirm
R3489	T5810	T5809	prep	for,role
R349	T1067	T1068	punct	[,1
R3490	T5811	T5810	pobj	Sam68,for
R3491	T5812	T5809	prep	in,role
R3492	T5813	T5814	det	the,regulation
R3493	T5814	T5812	pobj	regulation,in
R3494	T5815	T5814	prep	of,regulation
R3495	T5816	T5817	compound	adipocyte,differentiation
R3496	T5817	T5815	pobj	differentiation,of
R3497	T5818	T5807	punct	", ",isolated
R3498	T5819	T5807	nsubj	we,isolated
R3499	T5820	T5821	amod	primary,MEFs
R35	T272	T271	punct	-,type
R350	T1068	T1063	parataxis	1,activity
R3500	T5821	T5807	dobj	MEFs,isolated
R3501	T5822	T5821	prep	from,MEFs
R3502	T5823	T5824	nummod	14.5,day
R3503	T5824	T5826	npadvmod	day,old
R3504	T5825	T5824	punct	-,day
R3505	T5826	T5828	amod	old,embryos
R3506	T5827	T5826	punct	-,old
R3507	T5828	T5822	pobj	embryos,from
R3508	T5829	T5828	nmod	Sam68,embryos
R3509	T5830	T5829	punct	+,Sam68
R351	T1069	T1068	punct	],1
R3510	T5831	T5829	punct	/,Sam68
R3511	T5832	T5829	punct	+,Sam68
R3512	T5833	T5829	cc	and,Sam68
R3513	T5834	T5829	conj	Sam68,Sam68
R3514	T5835	T5834	punct	−,Sam68
R3515	T5836	T5834	punct	/,Sam68
R3516	T5837	T5834	punct	−,Sam68
R3517	T5838	T5807	punct	.,isolated
R3518	T5840	T5841	det	The,MEFs
R3519	T5841	T5847	nsubjpass	MEFs,differentiated
R352	T1070	T1058	auxpass	is,favored
R3520	T5842	T5841	amod	primary,MEFs
R3521	T5843	T5841	nmod	Sam68,MEFs
R3522	T5844	T5843	punct	−,Sam68
R3523	T5845	T5843	punct	/,Sam68
R3524	T5846	T5843	punct	−,Sam68
R3525	T5848	T5847	auxpass	were,differentiated
R3526	T5849	T5847	prep	into,differentiated
R3527	T5850	T5849	pobj	adipocytes,into
R3528	T5851	T5852	advmod	in,vitro
R3529	T5852	T5847	advmod	vitro,differentiated
R353	T1071	T1058	prep	over,favored
R3530	T5853	T5847	prep	in,differentiated
R3531	T5854	T5855	compound	culture,medium
R3532	T5855	T5853	pobj	medium,in
R3533	T5856	T5855	acl	containing,medium
R3534	T5857	T5858	nummod	5,μM
R3535	T5858	T5859	compound	μM,pioglitazone
R3536	T5859	T5856	dobj	pioglitazone,containing
R3537	T5860	T5861	aux	to,induce
R3538	T5861	T5847	advcl	induce,differentiated
R3539	T5862	T5861	dobj	adipogenesis,induce
R354	T1072	T1073	det	the,activity
R3540	T5863	T5847	punct	.,differentiated
R3541	T5865	T5866	nsubjpass	Cells,stained
R3542	T5867	T5865	prep	at,Cells
R3543	T5868	T5869	nmod	days,0
R3544	T5869	T5867	pobj	0,at
R3545	T5870	T5869	punct	", ",0
R3546	T5871	T5869	conj	4,0
R3547	T5872	T5871	punct	", ",4
R3548	T5873	T5871	conj	6,4
R3549	T5874	T5873	punct	", ",6
R355	T1073	T1071	pobj	activity,over
R3550	T5875	T5873	cc	and,6
R3551	T5876	T5873	conj	12,6
R3552	T5877	T5866	auxpass	were,stained
R3553	T5878	T5866	prep	with,stained
R3554	T5879	T5880	compound	Oil,O
R3555	T5880	T5878	pobj	O,with
R3556	T5881	T5880	compound	red,O
R3557	T5882	T5883	aux	to,monitor
R3558	T5883	T5866	advcl	monitor,stained
R3559	T5884	T5883	dobj	adipogenesis,monitor
R356	T1074	T1073	amod	catabolic,activity
R3560	T5885	T5866	punct	.,stained
R3561	T5887	T5888	nsubj	Adipogenesis,was
R3562	T5889	T5890	advmod	more,pronounced
R3563	T5890	T5888	acomp	pronounced,was
R3564	T5891	T5888	prep	in,was
R3565	T5892	T5893	det	the,cultures
R3566	T5893	T5891	pobj	cultures,in
R3567	T5894	T5895	amod	wild,type
R3568	T5895	T5893	compound	type,cultures
R3569	T5896	T5895	punct	-,type
R357	T1075	T1073	prep	of,activity
R3570	T5897	T5893	compound	MEFs,cultures
R3571	T5898	T5888	prep	than,was
R3572	T5899	T5898	prep	in,than
R3573	T5900	T5901	det	the,MEFs
R3574	T5901	T5899	pobj	MEFs,in
R3575	T5902	T5901	nmod	Sam68,MEFs
R3576	T5903	T5902	punct	−,Sam68
R3577	T5904	T5902	punct	/,Sam68
R3578	T5905	T5902	punct	−,Sam68
R3579	T5906	T5888	punct	", ",was
R358	T1076	T1075	pobj	osteoclasts,of
R3580	T5907	T5888	advcl	consistent,was
R3581	T5908	T5907	prep	with,consistent
R3582	T5909	T5910	det	the,role
R3583	T5910	T5908	pobj	role,with
R3584	T5911	T5910	amod	positive,role
R3585	T5912	T5910	prep	of,role
R3586	T5913	T5914	amod	endogenous,Sam68
R3587	T5914	T5912	pobj	Sam68,of
R3588	T5915	T5910	prep	in,role
R3589	T5916	T5917	compound	adipocyte,differentiation
R359	T1077	T1078	punct	[,2
R3590	T5917	T5915	pobj	differentiation,in
R3591	T5918	T5919	punct	(,Figure
R3592	T5919	T5888	parataxis	Figure,was
R3593	T5920	T5919	nummod	7,Figure
R3594	T5921	T5919	punct	),Figure
R3595	T5922	T5888	punct	.,was
R3596	T5924	T5925	det	The,expression
R3597	T5925	T5926	nsubjpass	expression,impaired
R3598	T5927	T5925	prep	of,expression
R3599	T5928	T5929	amod	key,factors
R36	T273	T274	compound	RNA,binding
R360	T1078	T1073	appos	2,activity
R3600	T5929	T5927	pobj	factors,of
R3601	T5930	T5929	compound	transcription,factors
R3602	T5931	T5929	prep	including,factors
R3603	T5932	T5933	det	the,PPARγ
R3604	T5933	T5931	pobj	PPARγ,including
R3605	T5934	T5933	cc	and,PPARγ
R3606	T5935	T5933	conj	KLF5,PPARγ
R3607	T5936	T5926	auxpass	was,impaired
R3608	T5937	T5926	prep	in,impaired
R3609	T5938	T5939	nmod	Sam68,MEFs
R361	T1079	T1078	punct	],2
R3610	T5939	T5937	pobj	MEFs,in
R3611	T5940	T5938	punct	−,Sam68
R3612	T5941	T5938	punct	/,Sam68
R3613	T5942	T5938	punct	−,Sam68
R3614	T5943	T5939	amod	differentiated,MEFs
R3615	T5944	T5926	prep	compared,impaired
R3616	T5945	T5944	prep	with,compared
R3617	T5946	T5947	nmod	Sam68,MEFs
R3618	T5947	T5945	pobj	MEFs,with
R3619	T5948	T5946	punct	+,Sam68
R362	T1080	T1073	punct	", ",activity
R3620	T5949	T5946	punct	/,Sam68
R3621	T5950	T5946	punct	+,Sam68
R3622	T5951	T5926	punct	", ",impaired
R3623	T5952	T5926	advcl	consistent,impaired
R3624	T5953	T5952	prep	with,consistent
R3625	T5954	T5955	amod	impaired,adipogenesis
R3626	T5955	T5953	pobj	adipogenesis,with
R3627	T5956	T5955	prep	in,adipogenesis
R3628	T5957	T5958	det	the,absence
R3629	T5958	T5956	pobj	absence,in
R363	T1081	T1082	dep	which,results
R3630	T5959	T5958	prep	of,absence
R3631	T5960	T5959	pobj	Sam68,of
R3632	T5961	T5962	punct	(,Figure
R3633	T5962	T5926	parataxis	Figure,impaired
R3634	T5963	T5962	nummod	7,Figure
R3635	T5964	T5962	punct	),Figure
R3636	T5965	T5926	punct	.,impaired
R3637	T5967	T5968	det	These,data
R3638	T5968	T5969	nsubj	data,support
R3639	T5970	T5968	punct	", ",data
R364	T1082	T1073	relcl	results,activity
R3640	T5971	T5968	advmod	together,data
R3641	T5972	T5971	prep	with,together
R3642	T5973	T5972	pobj	data,with
R3643	T5974	T5973	acl	confirming,data
R3644	T5975	T5976	det	a,phenotype
R3645	T5976	T5974	dobj	phenotype,confirming
R3646	T5977	T5976	amod	lean,phenotype
R3647	T5978	T5976	prep	in,phenotype
R3648	T5979	T5980	nmod	Sam68,mice
R3649	T5980	T5978	pobj	mice,in
R365	T1083	T1082	prep	in,results
R3650	T5981	T5979	punct	−,Sam68
R3651	T5982	T5979	punct	/,Sam68
R3652	T5983	T5979	punct	−,Sam68
R3653	T5984	T5985	punct	(,N.
R3654	T5985	T5968	meta	N.,data
R3655	T5986	T5985	nmod	Torabi,N.
R3656	T5987	T5985	cc	and,N.
R3657	T5988	T5985	conj	S.,N.
R3658	T5989	T5988	nmod	Richard,S.
R3659	T5990	T5988	punct	", ",S.
R366	T1084	T1085	det	a,gain
R3660	T5991	T5992	amod	unpublished,data
R3661	T5992	T5988	conj	data,S.
R3662	T5993	T5992	punct	),data
R3663	T5994	T5969	punct	", ",support
R3664	T5995	T5996	det	the,hypothesis
R3665	T5996	T5969	dobj	hypothesis,support
R3666	T5997	T5998	mark	that,modulates
R3667	T5998	T5996	acl	modulates,hypothesis
R3668	T5999	T5998	nsubj	Sam68,modulates
R3669	T6000	T6001	det	the,differentiation
R367	T1085	T1083	pobj	gain,in
R3670	T6001	T5998	dobj	differentiation,modulates
R3671	T6002	T6001	prep	of,differentiation
R3672	T6003	T6004	amod	mesenchymal,cells
R3673	T6004	T6002	pobj	cells,of
R3674	T6005	T5969	punct	.,support
R3675	T6007	T6008	aux	To,examine
R3676	T6008	T6010	advcl	examine,chose
R3677	T6009	T6008	advmod	further,examine
R3678	T6011	T6012	det	this,phenotype
R3679	T6012	T6008	dobj	phenotype,examine
R368	T1086	T1085	amod	net,gain
R3680	T6013	T6008	prep	in,examine
R3681	T6014	T6015	det	a,system
R3682	T6015	T6013	pobj	system,in
R3683	T6016	T6017	npadvmod	cell,autonomous
R3684	T6017	T6015	amod	autonomous,system
R3685	T6018	T6010	punct	", ",chose
R3686	T6019	T6010	nsubj	we,chose
R3687	T6020	T6021	det	the,cells
R3688	T6021	T6010	dobj	cells,chose
R3689	T6022	T6021	amod	embryonic,cells
R369	T1087	T1085	prep	in,gain
R3690	T6023	T6021	amod	mesenchymal,cells
R3691	T6024	T6021	amod	multipotential,cells
R3692	T6025	T6021	compound	progenitor,cells
R3693	T6026	T6021	appos	C3H10T1,cells
R3694	T6027	T6026	punct	/,C3H10T1
R3695	T6028	T6026	nummod	2,C3H10T1
R3696	T6029	T6010	punct	.,chose
R3697	T6031	T6032	det	The,addition
R3698	T6032	T6033	nsubj	addition,induced
R3699	T6034	T6032	prep	of,addition
R37	T274	T269	compound	binding,protein
R370	T1088	T1089	compound	bone,mass
R3700	T6035	T6034	pobj	BMP,of
R3701	T6036	T6035	punct	-,BMP
R3702	T6037	T6035	nummod	2,BMP
R3703	T6038	T6039	compound	osteoblast,differentiation
R3704	T6039	T6033	dobj	differentiation,induced
R3705	T6040	T6033	punct	", ",induced
R3706	T6041	T6042	mark	as,evidenced
R3707	T6042	T6033	advcl	evidenced,induced
R3708	T6043	T6042	agent	by,evidenced
R3709	T6044	T6045	det	an,increase
R371	T1089	T1087	pobj	mass,in
R3710	T6045	T6043	pobj	increase,by
R3711	T6046	T6047	advmod	approximately,400-fold
R3712	T6047	T6045	nmod	400-fold,increase
R3713	T6048	T6045	prep	in,increase
R3714	T6049	T6048	pobj	expression,in
R3715	T6050	T6049	prep	of,expression
R3716	T6051	T6052	det	the,gene
R3717	T6052	T6050	pobj	gene,of
R3718	T6053	T6052	nmod	osteocalcin,gene
R3719	T6054	T6053	punct	(,osteocalcin
R372	T1090	T1058	punct	.,favored
R3720	T6055	T6053	appos	OCN,osteocalcin
R3721	T6056	T6052	punct	),gene
R3722	T6057	T6058	punct	[,51
R3723	T6058	T6033	parataxis	51,induced
R3724	T6059	T6058	punct	],51
R3725	T6060	T6033	punct	.,induced
R3726	T6062	T6063	nsubjpass	Populations,selected
R3727	T6064	T6062	prep	of,Populations
R3728	T6065	T6066	nmod	C3H10T1,cells
R3729	T6066	T6064	pobj	cells,of
R373	T1092	T1093	prep	At,maintained
R3730	T6067	T6065	punct	/,C3H10T1
R3731	T6068	T6065	nummod	2,C3H10T1
R3732	T6069	T6070	advmod	stably,transfected
R3733	T6070	T6062	acl	transfected,Populations
R3734	T6071	T6070	prep	with,transfected
R3735	T6072	T6073	preconj	either,retro
R3736	T6073	T6071	pobj	retro,with
R3737	T6074	T6073	compound	pSuper,retro
R3738	T6075	T6073	punct	-,retro
R3739	T6076	T6077	punct	(,control
R374	T1094	T1095	amod	skeletal,maturity
R3740	T6077	T6073	parataxis	control,retro
R3741	T6078	T6077	punct	),control
R3742	T6079	T6073	cc	and,retro
R3743	T6080	T6081	compound	pSuper,retro
R3744	T6081	T6073	conj	retro,retro
R3745	T6082	T6081	punct	-,retro
R3746	T6083	T6081	acl	harboring,retro
R3747	T6084	T6085	det	a,hairpin
R3748	T6085	T6083	dobj	hairpin,harboring
R3749	T6086	T6085	amod	short,hairpin
R375	T1095	T1092	pobj	maturity,At
R3750	T6087	T6085	prep	against,hairpin
R3751	T6088	T6087	pobj	Sam68,against
R3752	T6089	T6085	punct	(,hairpin
R3753	T6090	T6085	appos	Sam68sh,hairpin
R3754	T6091	T6063	punct	),selected
R3755	T6092	T6063	auxpass	were,selected
R3756	T6093	T6063	prep	with,selected
R3757	T6094	T6093	pobj	puromycin,with
R3758	T6095	T6063	punct	.,selected
R3759	T6097	T6098	det	The,expression
R376	T1096	T1093	punct	", ",maintained
R3760	T6098	T6099	nsubjpass	expression,reduced
R3761	T6100	T6098	prep	of,expression
R3762	T6101	T6100	pobj	Sam68,of
R3763	T6102	T6099	auxpass	was,reduced
R3764	T6103	T6099	prep	by,reduced
R3765	T6104	T6105	advmod	approximately,80
R3766	T6105	T6106	nummod	80,%
R3767	T6106	T6103	pobj	%,by
R3768	T6107	T6108	mark	as,evidenced
R3769	T6108	T6099	advcl	evidenced,reduced
R377	T1097	T1098	compound	bone,mass
R3770	T6109	T6108	agent	by,evidenced
R3771	T6110	T6111	compound	immunoblot,analyses
R3772	T6111	T6109	pobj	analyses,by
R3773	T6112	T6111	acl	using,analyses
R3774	T6113	T6114	compound	β,actin
R3775	T6114	T6112	dobj	actin,using
R3776	T6115	T6114	punct	-,actin
R3777	T6116	T6112	prep	as,using
R3778	T6117	T6118	det	a,control
R3779	T6118	T6116	pobj	control,as
R378	T1098	T1093	nsubjpass	mass,maintained
R3780	T6119	T6118	compound	loading,control
R3781	T6120	T6121	punct	(,8A
R3782	T6121	T6099	parataxis	8A,reduced
R3783	T6122	T6121	compound	Figure,8A
R3784	T6123	T6121	punct	),8A
R3785	T6124	T6099	punct	.,reduced
R3786	T6126	T6127	compound	Osteoblast,differentiation
R3787	T6127	T6128	nsubjpass	differentiation,induced
R3788	T6129	T6128	auxpass	was,induced
R3789	T6130	T6128	prep	in,induced
R379	T1099	T1093	auxpass	is,maintained
R3790	T6131	T6130	pobj	cultures,in
R3791	T6132	T6131	acl	expressing,cultures
R3792	T6133	T6134	compound	pSuper,retro
R3793	T6134	T6132	dobj	retro,expressing
R3794	T6135	T6134	punct	-,retro
R3795	T6136	T6134	cc	or,retro
R3796	T6137	T6138	compound	pSuper,retro
R3797	T6138	T6140	compound	retro,Sam68sh
R3798	T6139	T6138	punct	-,retro
R3799	T6140	T6134	conj	Sam68sh,retro
R38	T275	T276	dep	that,shown
R380	T1100	T1093	prep	through,maintained
R3800	T6141	T6128	prep	by,induced
R3801	T6142	T6141	pobj	addition,by
R3802	T6143	T6142	prep	of,addition
R3803	T6144	T6143	pobj	BMP,of
R3804	T6145	T6144	punct	-,BMP
R3805	T6146	T6144	nummod	2,BMP
R3806	T6147	T6142	prep	to,addition
R3807	T6148	T6149	det	the,medium
R3808	T6149	T6147	pobj	medium,to
R3809	T6150	T6149	compound	culture,medium
R381	T1101	T1102	det	the,activity
R3810	T6151	T6128	punct	.,induced
R3811	T6152	T6128	punct	.,induced
R3812	T6154	T6155	det	The,expression
R3813	T6155	T6156	nsubjpass	expression,examined
R3814	T6157	T6155	prep	of,expression
R3815	T6158	T6157	pobj	OCN,of
R3816	T6159	T6158	cc	and,OCN
R3817	T6160	T6161	compound	β,actin
R3818	T6161	T6163	compound	actin,mRNAs
R3819	T6162	T6161	punct	-,actin
R382	T1102	T1100	pobj	activity,through
R3820	T6163	T6158	conj	mRNAs,OCN
R3821	T6164	T6156	auxpass	was,examined
R3822	T6165	T6156	prep	by,examined
R3823	T6166	T6167	amod	semiquantitative,PCR
R3824	T6167	T6165	pobj	PCR,by
R3825	T6168	T6167	compound	RT,PCR
R3826	T6169	T6167	punct	-,PCR
R3827	T6170	T6156	cc	and,examined
R3828	T6171	T6172	det	the,cells
R3829	T6172	T6176	nsubj	cells,displayed
R383	T1103	T1102	amod	balanced,activity
R3830	T6173	T6174	npadvmod	Sam68sh,expressing
R3831	T6174	T6172	amod	expressing,cells
R3832	T6175	T6174	punct	-,expressing
R3833	T6176	T6156	conj	displayed,examined
R3834	T6177	T6178	det	a,phenotype
R3835	T6178	T6176	dobj	phenotype,displayed
R3836	T6179	T6180	advmod	more,pronounced
R3837	T6180	T6178	amod	pronounced,phenotype
R3838	T6181	T6178	compound	osteoblast,phenotype
R3839	T6182	T6178	prep	compared,phenotype
R384	T1104	T1102	prep	of,activity
R3840	T6183	T6182	prep	with,compared
R3841	T6184	T6185	compound	control,cells
R3842	T6185	T6183	pobj	cells,with
R3843	T6186	T6176	punct	", ",displayed
R3844	T6187	T6188	mark	as,assessed
R3845	T6188	T6176	advcl	assessed,displayed
R3846	T6189	T6188	prep	by,assessed
R3847	T6190	T6191	det	the,expression
R3848	T6191	T6189	pobj	expression,by
R3849	T6192	T6191	prep	of,expression
R385	T1105	T1104	pobj	osteoblasts,of
R3850	T6193	T6192	pobj	OCN,of
R3851	T6194	T6195	punct	(,8B
R3852	T6195	T6176	parataxis	8B,displayed
R3853	T6196	T6195	compound	Figure,8B
R3854	T6197	T6195	punct	),8B
R3855	T6198	T6176	punct	.,displayed
R3856	T6200	T6201	prep	Given,stained
R3857	T6202	T6203	det	the,enhancement
R3858	T6203	T6200	pobj	enhancement,Given
R3859	T6204	T6203	amod	apparent,enhancement
R386	T1106	T1105	cc	and,osteoblasts
R3860	T6205	T6203	prep	of,enhancement
R3861	T6206	T6207	amod	mineralized,nodule
R3862	T6207	T6208	compound	nodule,formation
R3863	T6208	T6205	pobj	formation,of
R3864	T6209	T6203	prep	by,enhancement
R3865	T6210	T6211	nmod	Sam68,cells
R3866	T6211	T6209	pobj	cells,by
R3867	T6212	T6210	punct	−,Sam68
R3868	T6213	T6210	punct	/,Sam68
R3869	T6214	T6210	punct	−,Sam68
R387	T1107	T1105	conj	osteoclasts,osteoblasts
R3870	T6215	T6216	nmod	bone,marrow
R3871	T6216	T6211	nmod	marrow,cells
R3872	T6217	T6211	amod	stromal,cells
R3873	T6218	T6219	advmod	ex,vivo
R3874	T6219	T6203	advmod	vivo,enhancement
R3875	T6220	T6203	cc	and,enhancement
R3876	T6221	T6222	det	the,phenotype
R3877	T6222	T6203	conj	phenotype,enhancement
R3878	T6223	T6222	acl	observed,phenotype
R3879	T6224	T6223	prep	with,observed
R388	T1108	T1093	prep	during,maintained
R3880	T6225	T6226	amod	short,RNA
R3881	T6226	T6228	npadvmod	RNA,treated
R3882	T6227	T6226	compound	hairpin,RNA
R3883	T6228	T6233	amod	treated,C3H10T1
R3884	T6229	T6226	punct	(,RNA
R3885	T6230	T6226	appos	shRNA,RNA
R3886	T6231	T6228	punct	),treated
R3887	T6232	T6228	punct	-,treated
R3888	T6233	T6224	pobj	C3H10T1,with
R3889	T6234	T6233	punct	/,C3H10T1
R389	T1109	T1110	det	the,cycle
R3890	T6235	T6233	nummod	2,C3H10T1
R3891	T6236	T6201	punct	", ",stained
R3892	T6237	T6201	nsubj	we,stained
R3893	T6238	T6201	dobj	sections,stained
R3894	T6239	T6238	prep	of,sections
R3895	T6240	T6239	pobj	bone,of
R3896	T6241	T6238	prep	from,sections
R3897	T6242	T6243	nummod	4,month
R3898	T6243	T6248	npadvmod	month,old
R3899	T6244	T6242	punct	-,4
R39	T276	T269	relcl	shown,protein
R390	T1110	T1108	pobj	cycle,during
R3900	T6245	T6242	cc	and,4
R3901	T6246	T6242	conj	12,4
R3902	T6247	T6243	punct	-,month
R3903	T6248	T6250	amod	old,mice
R3904	T6249	T6248	punct	-,old
R3905	T6250	T6241	pobj	mice,from
R3906	T6251	T6201	prep	for,stained
R3907	T6252	T6251	pobj	evidence,for
R3908	T6253	T6252	prep	of,evidence
R3909	T6254	T6253	pobj	changes,of
R391	T1111	T1110	compound	remodeling,cycle
R3910	T6255	T6254	prep	in,changes
R3911	T6256	T6257	compound	marrow,adiposity
R3912	T6257	T6255	pobj	adiposity,in
R3913	T6258	T6201	punct	.,stained
R3914	T6260	T6261	nsubj	Figure,shows
R3915	T6262	T6260	nummod	9,Figure
R3916	T6263	T6261	dobj	sections,shows
R3917	T6264	T6263	prep	of,sections
R3918	T6265	T6266	amod	undecalcified,bone
R3919	T6266	T6264	pobj	bone,of
R392	T1112	T1093	punct	.,maintained
R3920	T6267	T6266	prep	from,bone
R3921	T6268	T6269	nummod	4,month
R3922	T6269	T6274	npadvmod	month,old
R3923	T6270	T6268	punct	-,4
R3924	T6271	T6268	cc	and,4
R3925	T6272	T6268	conj	12,4
R3926	T6273	T6269	punct	-,month
R3927	T6274	T6276	amod	old,mice
R3928	T6275	T6274	punct	-,old
R3929	T6276	T6267	pobj	mice,from
R393	T1114	T1115	prep	During,is
R3930	T6277	T6276	nmod	Sam68,mice
R3931	T6278	T6277	punct	+,Sam68
R3932	T6279	T6277	punct	/,Sam68
R3933	T6280	T6277	punct	+,Sam68
R3934	T6281	T6277	cc	and,Sam68
R3935	T6282	T6277	conj	Sam68,Sam68
R3936	T6283	T6282	punct	−,Sam68
R3937	T6284	T6282	punct	/,Sam68
R3938	T6285	T6282	punct	−,Sam68
R3939	T6286	T6276	acl	stained,mice
R394	T1116	T1117	amod	skeletal,aging
R3940	T6287	T6286	prep	with,stained
R3941	T6288	T6289	compound	von,Kossa
R3942	T6289	T6287	pobj	Kossa,with
R3943	T6290	T6289	cc	and,Kossa
R3944	T6291	T6289	conj	toluidine,Kossa
R3945	T6292	T6291	amod	blue,toluidine
R3946	T6293	T6294	aux	to,show
R3947	T6294	T6286	advcl	show,stained
R3948	T6295	T6296	amod	mineralized,tissue
R3949	T6296	T6294	dobj	tissue,show
R395	T1117	T1114	pobj	aging,During
R3950	T6297	T6298	punct	(,9A
R3951	T6298	T6296	parataxis	9A,tissue
R3952	T6299	T6298	compound	Figure,9A
R3953	T6300	T6298	punct	", ",9A
R3954	T6301	T6298	amod	black,9A
R3955	T6302	T6298	punct	),9A
R3956	T6303	T6296	cc	and,tissue
R3957	T6304	T6305	compound	marrow,adipocytes
R3958	T6305	T6296	conj	adipocytes,tissue
R3959	T6306	T6307	punct	(,9B
R396	T1118	T1115	punct	", ",is
R3960	T6307	T6305	parataxis	9B,adipocytes
R3961	T6308	T6307	compound	Figure,9B
R3962	T6309	T6307	punct	", ",9B
R3963	T6310	T6307	amod	white,9B
R3964	T6311	T6307	punct	),9B
R3965	T6312	T6286	cc	and,stained
R3966	T6313	T6286	conj	left,stained
R3967	T6314	T6313	oprd	unstained,left
R3968	T6315	T6316	aux	to,show
R3969	T6316	T6313	advcl	show,left
R397	T1119	T1115	expl	there,is
R3970	T6317	T6318	compound	fluorochrome,labeling
R3971	T6318	T6316	dobj	labeling,show
R3972	T6319	T6318	prep	of,labeling
R3973	T6320	T6321	det	the,fronts
R3974	T6321	T6319	pobj	fronts,of
R3975	T6322	T6321	compound	mineralization,fronts
R3976	T6323	T6324	punct	(,9C
R3977	T6324	T6316	parataxis	9C,show
R3978	T6325	T6324	compound	Figure,9C
R3979	T6326	T6324	punct	),9C
R398	T1120	T1121	det	a,shift
R3980	T6327	T6261	punct	.,shows
R3981	T6329	T6330	nummod	Twelve,month
R3982	T6330	T6332	npadvmod	month,old
R3983	T6331	T6330	punct	-,month
R3984	T6332	T6334	amod	old,mice
R3985	T6333	T6332	punct	-,old
R3986	T6334	T6339	nsubj	mice,showed
R3987	T6335	T6334	nmod	Sam68,mice
R3988	T6336	T6335	punct	+,Sam68
R3989	T6337	T6335	punct	/,Sam68
R399	T1121	T1115	attr	shift,is
R3990	T6338	T6335	punct	+,Sam68
R3991	T6340	T6341	det	a,decrease
R3992	T6341	T6339	dobj	decrease,showed
R3993	T6342	T6341	amod	noticeable,decrease
R3994	T6343	T6341	prep	in,decrease
R3995	T6344	T6345	amod	trabecular,bone
R3996	T6345	T6343	pobj	bone,in
R3997	T6346	T6347	punct	(,9A
R3998	T6347	T6345	parataxis	9A,bone
R3999	T6348	T6347	compound	Figure,9A
R40	T277	T276	aux	has,shown
R400	T1122	T1121	prep	in,shift
R4000	T6349	T6347	punct	),9A
R4001	T6350	T6341	punct	", ",decrease
R4002	T6351	T6352	dep	which,associated
R4003	T6352	T6341	relcl	associated,decrease
R4004	T6353	T6352	auxpass	was,associated
R4005	T6354	T6352	prep	with,associated
R4006	T6355	T6356	det	a,increase
R4007	T6356	T6354	pobj	increase,with
R4008	T6357	T6356	amod	significant,increase
R4009	T6358	T6356	prep	in,increase
R401	T1123	T1124	det	the,balance
R4010	T6359	T6360	compound	marrow,adiposity
R4011	T6360	T6358	pobj	adiposity,in
R4012	T6361	T6362	punct	(,9B
R4013	T6362	T6356	parataxis	9B,increase
R4014	T6363	T6362	compound	Figure,9B
R4015	T6364	T6362	punct	),9B
R4016	T6365	T6354	cc	and,with
R4017	T6366	T6354	conj	with,with
R4018	T6367	T6368	det	the,labels
R4019	T6368	T6366	pobj	labels,with
R402	T1124	T1122	pobj	balance,in
R4020	T6369	T6368	nummod	two,labels
R4021	T6370	T6368	compound	fluorochrome,labels
R4022	T6371	T6368	acl	superimposed,labels
R4023	T6372	T6371	prep	upon,superimposed
R4024	T6373	T6372	pobj	one,upon
R4025	T6374	T6373	det	another,one
R4026	T6375	T6376	punct	(,9C
R4027	T6376	T6368	parataxis	9C,labels
R4028	T6377	T6376	compound	Figure,9C
R4029	T6378	T6376	punct	),9C
R403	T1125	T1126	dep	that,favors
R4030	T6379	T6339	punct	.,showed
R4031	T6381	T6382	prep	In,appeared
R4032	T6383	T6381	pobj	contrast,In
R4033	T6384	T6382	punct	", ",appeared
R4034	T6385	T6386	det	the,bones
R4035	T6386	T6382	nsubj	bones,appeared
R4036	T6387	T6386	prep	of,bones
R4037	T6388	T6389	nummod	12,month
R4038	T6389	T6391	npadvmod	month,old
R4039	T6390	T6389	punct	-,month
R404	T1126	T1121	relcl	favors,shift
R4040	T6391	T6393	amod	old,mice
R4041	T6392	T6391	punct	-,old
R4042	T6393	T6387	pobj	mice,of
R4043	T6394	T6393	nmod	Sam68,mice
R4044	T6395	T6394	punct	−,Sam68
R4045	T6396	T6394	punct	/,Sam68
R4046	T6397	T6394	punct	−,Sam68
R4047	T6398	T6382	oprd	similar,appeared
R4048	T6399	T6398	prep	to,similar
R4049	T6400	T6399	pobj	those,to
R405	T1127	T1126	dative	osteoclast,favors
R4050	T6401	T6400	prep	of,those
R4051	T6402	T6403	det	the,mice
R4052	T6403	T6401	pobj	mice,of
R4053	T6404	T6405	nummod	4,month
R4054	T6405	T6407	npadvmod	month,old
R4055	T6406	T6405	punct	-,month
R4056	T6407	T6403	amod	old,mice
R4057	T6408	T6407	punct	-,old
R4058	T6409	T6403	prep	of,mice
R4059	T6410	T6411	det	either,genotype
R406	T1128	T1126	prep	over,favors
R4060	T6411	T6409	pobj	genotype,of
R4061	T6412	T6382	punct	.,appeared
R4062	T6414	T6415	det	These,data
R4063	T6415	T6416	nsubj	data,demonstrate
R4064	T6417	T6418	mark	that,regulates
R4065	T6418	T6416	ccomp	regulates,demonstrate
R4066	T6419	T6418	nsubj	Sam68,regulates
R4067	T6420	T6421	det	the,differentiation
R4068	T6421	T6418	dobj	differentiation,regulates
R4069	T6422	T6421	prep	of,differentiation
R407	T1129	T1128	pobj	osteoblast,over
R4070	T6423	T6424	nmod	bone,marrow
R4071	T6424	T6425	nmod	marrow,cells
R4072	T6425	T6422	pobj	cells,of
R4073	T6426	T6425	amod	mesenchymal,cells
R4074	T6427	T6428	aux	to,promote
R4075	T6428	T6418	advcl	promote,regulates
R4076	T6429	T6430	compound	adipocyte,differentiation
R4077	T6430	T6428	dobj	differentiation,promote
R4078	T6431	T6428	cc	and,promote
R4079	T6432	T6428	conj	inhibit,promote
R408	T1130	T1126	dobj	activity,favors
R4080	T6433	T6434	compound	osteoblast,differentiation
R4081	T6434	T6432	dobj	differentiation,inhibit
R4082	T6435	T6428	prep	in,promote
R4083	T6436	T6437	amod	aging,bone
R4084	T6437	T6435	pobj	bone,in
R4085	T6438	T6416	punct	.,demonstrate
R409	T1131	T1126	punct	", ",favors
R41	T278	T276	auxpass	been,shown
R410	T1132	T1133	dep	which,results
R4104	T7333	T7334	det	The,study
R4105	T7334	T7336	nsubj	study,provides
R4106	T7335	T7334	amod	present,study
R4107	T7337	T7336	dobj	evidence,provides
R4108	T7338	T7339	mark	that,is
R4109	T7339	T7337	acl	is,evidence
R411	T1133	T1126	ccomp	results,favors
R4110	T7340	T7341	det	a,role
R4111	T7341	T7339	nsubj	role,is
R4112	T7342	T7341	amod	physiologic,role
R4113	T7343	T7341	prep	of,role
R4114	T7344	T7343	pobj	Sam68,of
R4115	T7345	T7346	aux	to,modulate
R4116	T7346	T7339	xcomp	modulate,is
R4117	T7347	T7348	nmod	bone,marrow
R4118	T7348	T7349	nmod	marrow,cells
R4119	T7349	T7346	dobj	cells,modulate
R412	T1134	T1133	prep	in,results
R4120	T7350	T7349	amod	mesenchymal,cells
R4121	T7351	T7349	compound	stem,cells
R4122	T7352	T7336	punct	.,provides
R4123	T7354	T7355	amod	Young,mice
R4124	T7355	T7360	nsubj	mice,developed
R4125	T7356	T7355	nmod	Sam68,mice
R4126	T7357	T7356	punct	−,Sam68
R4127	T7358	T7356	punct	/,Sam68
R4128	T7359	T7356	punct	−,Sam68
R4129	T7361	T7360	advmod	normally,developed
R413	T1135	T1136	amod	net,loss
R4130	T7362	T7360	cc	and,developed
R4131	T7363	T7360	conj	contained,developed
R4132	T7364	T7365	amod	similar,mass
R4133	T7365	T7363	dobj	mass,contained
R4134	T7366	T7365	compound	bone,mass
R4135	T7367	T7363	prep	compared,contained
R4136	T7368	T7367	prep	with,compared
R4137	T7369	T7370	amod	wild,type
R4138	T7370	T7372	compound	type,littermates
R4139	T7371	T7370	punct	-,type
R414	T1136	T1134	pobj	loss,in
R4140	T7372	T7368	pobj	littermates,with
R4141	T7373	T7360	punct	.,developed
R4142	T7375	T7376	amod	Aged,mice
R4143	T7376	T7385	nsubj	mice,displayed
R4144	T7377	T7378	punct	(,months
R4145	T7378	T7375	parataxis	months,Aged
R4146	T7379	T7378	nummod	12,months
R4147	T7380	T7378	punct	),months
R4148	T7381	T7376	nmod	Sam68,mice
R4149	T7382	T7381	punct	−,Sam68
R415	T1137	T1136	compound	bone,loss
R4150	T7383	T7381	punct	/,Sam68
R4151	T7384	T7381	punct	−,Sam68
R4152	T7386	T7387	det	a,phenotype
R4153	T7387	T7385	dobj	phenotype,displayed
R4154	T7388	T7389	amod	high,mass
R4155	T7389	T7387	compound	mass,phenotype
R4156	T7390	T7389	compound	bone,mass
R4157	T7391	T7385	prep	compared,displayed
R4158	T7392	T7391	prep	with,compared
R4159	T7393	T7394	nmod	Sam68,littermates
R416	T1138	T1139	punct	[,3
R4160	T7394	T7392	pobj	littermates,with
R4161	T7395	T7393	punct	+,Sam68
R4162	T7396	T7393	punct	/,Sam68
R4163	T7397	T7393	punct	+,Sam68
R4164	T7398	T7385	punct	.,displayed
R4165	T7400	T7401	det	The,littermates
R4166	T7401	T7405	nsubj	littermates,underwent
R4167	T7402	T7403	amod	wild,type
R4168	T7403	T7401	compound	type,littermates
R4169	T7404	T7403	punct	-,type
R417	T1139	T1115	parataxis	3,is
R4170	T7406	T7407	npadvmod	age,related
R4171	T7407	T7409	amod	related,loss
R4172	T7408	T7407	punct	-,related
R4173	T7409	T7405	dobj	loss,underwent
R4174	T7410	T7409	compound	bone,loss
R4175	T7411	T7412	dep	that,occurs
R4176	T7412	T7409	relcl	occurs,loss
R4177	T7413	T7412	advmod	naturally,occurs
R4178	T7414	T7412	prep	in,occurs
R4179	T7415	T7414	pobj	mammals,in
R418	T1140	T1139	punct	],3
R4180	T7416	T7405	punct	", ",underwent
R4181	T7417	T7418	mark	while,preserved
R4182	T7418	T7405	advcl	preserved,underwent
R4183	T7419	T7420	det	the,animals
R4184	T7420	T7418	nsubj	animals,preserved
R4185	T7421	T7420	nmod	Sam68,animals
R4186	T7422	T7421	punct	−,Sam68
R4187	T7423	T7421	punct	/,Sam68
R4188	T7424	T7421	punct	−,Sam68
R4189	T7425	T7426	poss	their,mass
R419	T1141	T1115	punct	.,is
R4190	T7426	T7418	dobj	mass,preserved
R4191	T7427	T7426	compound	bone,mass
R4192	T7428	T7418	prep	with,preserved
R4193	T7429	T7428	pobj	aging,with
R4194	T7430	T7405	punct	.,underwent
R4195	T7432	T7433	det	The,differentiation
R4196	T7433	T7434	nsubj	differentiation,resulted
R4197	T7435	T7433	prep	of,differentiation
R4198	T7436	T7437	compound	bone,marrow
R4199	T7437	T7438	compound	marrow,cells
R42	T279	T280	aux	to,regulate
R420	T1143	T1144	det	The,amount
R4200	T7438	T7435	pobj	cells,of
R4201	T7439	T7438	compound	stem,cells
R4202	T7440	T7438	acl	isolated,cells
R4203	T7441	T7440	prep	from,isolated
R4204	T7442	T7443	nmod	Sam68,mice
R4205	T7443	T7441	pobj	mice,from
R4206	T7444	T7442	punct	−,Sam68
R4207	T7445	T7442	punct	/,Sam68
R4208	T7446	T7442	punct	−,Sam68
R4209	T7447	T7438	cc	and,cells
R421	T1144	T1145	nsubjpass	amount,determined
R4210	T7448	T7449	amod	embryonic,cells
R4211	T7449	T7438	conj	cells,cells
R4212	T7450	T7449	amod	mesenchymal,cells
R4213	T7451	T7449	amod	multipotential,cells
R4214	T7452	T7449	compound	progenitor,cells
R4215	T7453	T7449	appos	C3H10T1,cells
R4216	T7454	T7453	punct	/,C3H10T1
R4217	T7455	T7453	nummod	2,C3H10T1
R4218	T7456	T7449	acl	treated,cells
R4219	T7457	T7456	prep	with,treated
R422	T1146	T1144	cc	and,amount
R4220	T7458	T7459	compound	Sam68,shRNA
R4221	T7459	T7457	pobj	shRNA,with
R4222	T7460	T7434	prep	in,resulted
R4223	T7461	T7462	det	a,differentiation
R4224	T7462	T7460	pobj	differentiation,in
R4225	T7463	T7464	advmod	more,pronounced
R4226	T7464	T7462	amod	pronounced,differentiation
R4227	T7465	T7462	compound	osteoblast,differentiation
R4228	T7466	T7434	punct	.,resulted
R4229	T7468	T7469	det	These,findings
R423	T1147	T1144	conj	rate,amount
R4230	T7469	T7470	nsubj	findings,demonstrate
R4231	T7471	T7472	mark	that,enhances
R4232	T7472	T7470	ccomp	enhances,demonstrate
R4233	T7473	T7474	det	the,loss
R4234	T7474	T7472	nsubj	loss,enhances
R4235	T7475	T7474	prep	of,loss
R4236	T7476	T7475	pobj	Sam68,of
R4237	T7477	T7478	compound	osteoblast,differentiation
R4238	T7478	T7472	dobj	differentiation,enhances
R4239	T7479	T7470	punct	.,demonstrate
R424	T1148	T1149	prep	at,gained
R4240	T7481	T7482	det	The,converse
R4241	T7482	T7483	nsubj	converse,was
R4242	T7484	T7483	advmod	also,was
R4243	T7485	T7483	acomp	true,was
R4244	T7486	T7483	punct	", ",was
R4245	T7487	T7488	mark	as,impaired
R4246	T7488	T7483	advcl	impaired,was
R4247	T7489	T7488	nsubjpass	MEFs,impaired
R4248	T7490	T7489	acl	isolated,MEFs
R4249	T7491	T7490	prep	from,isolated
R425	T1149	T1147	relcl	gained,rate
R4250	T7492	T7493	nmod	Sam68,animals
R4251	T7493	T7491	pobj	animals,from
R4252	T7494	T7492	punct	−,Sam68
R4253	T7495	T7492	punct	/,Sam68
R4254	T7496	T7492	punct	−,Sam68
R4255	T7497	T7488	auxpass	were,impaired
R4256	T7498	T7488	prep	in,impaired
R4257	T7499	T7500	poss	their,ability
R4258	T7500	T7498	pobj	ability,in
R4259	T7501	T7502	aux	to,undergo
R426	T1150	T1148	pobj	which,at
R4260	T7502	T7500	acl	undergo,ability
R4261	T7503	T7504	compound	adipocyte,differentiation
R4262	T7504	T7502	dobj	differentiation,undergo
R4263	T7505	T7488	prep	compared,impaired
R4264	T7506	T7505	prep	with,compared
R4265	T7507	T7508	nmod	Sam68,MEFs
R4266	T7508	T7506	pobj	MEFs,with
R4267	T7509	T7507	punct	+,Sam68
R4268	T7510	T7507	punct	/,Sam68
R4269	T7511	T7507	punct	+,Sam68
R427	T1151	T1149	nsubjpass	bone,gained
R4270	T7512	T7483	punct	.,was
R4271	T7514	T7515	det	These,findings
R4272	T7515	T7516	nsubj	findings,suggest
R4273	T7517	T7518	mark	that,is
R4274	T7518	T7516	ccomp	is,suggest
R4275	T7519	T7520	det	a,role
R4276	T7520	T7518	nsubj	role,is
R4277	T7521	T7520	amod	physiologic,role
R4278	T7522	T7520	prep	for,role
R4279	T7523	T7522	pobj	Sam68,for
R428	T1152	T1149	auxpass	is,gained
R4280	T7524	T7525	aux	to,regulate
R4281	T7525	T7518	xcomp	regulate,is
R4282	T7526	T7527	det	the,balance
R4283	T7527	T7525	dobj	balance,regulate
R4284	T7528	T7527	prep	between,balance
R4285	T7529	T7530	amod	adipogenic,differentiation
R4286	T7530	T7528	pobj	differentiation,between
R4287	T7531	T7529	cc	and,adipogenic
R4288	T7532	T7529	conj	osteogenic,adipogenic
R4289	T7533	T7530	prep	of,differentiation
R429	T1153	T1149	prep	during,gained
R4290	T7534	T7535	det	the,marrow
R4291	T7535	T7533	pobj	marrow,of
R4292	T7536	T7535	compound	bone,marrow
R4293	T7537	T7535	amod	mesenchymal,marrow
R4294	T7538	T7516	punct	.,suggest
R4295	T7540	T7541	compound	Sam68,expression
R4296	T7541	T7543	nsubjpass	expression,observed
R4297	T7542	T7541	compound	protein,expression
R4298	T7544	T7543	auxpass	was,observed
R4299	T7545	T7543	prep	throughout,observed
R43	T280	T276	xcomp	regulate,shown
R430	T1154	T1153	pobj	development,during
R4300	T7546	T7547	det	the,embryo
R4301	T7547	T7545	pobj	embryo,throughout
R4302	T7548	T7547	amod	developing,embryo
R4303	T7549	T7547	compound	mouse,embryo
R4304	T7550	T7543	punct	", ",observed
R4305	T7551	T7543	prep	in,observed
R4306	T7552	T7551	pobj	keeping,in
R4307	T7553	T7552	prep	with,keeping
R4308	T7554	T7555	amod	previous,reports
R4309	T7555	T7553	pobj	reports,with
R431	T1155	T1149	cc	and,gained
R4310	T7556	T7557	dep	that,identified
R4311	T7557	T7555	relcl	identified,reports
R4312	T7558	T7559	det	the,mRNA
R4313	T7559	T7561	nsubjpass	mRNA,expressed
R4314	T7560	T7559	compound	Sam68,mRNA
R4315	T7561	T7557	ccomp	expressed,identified
R4316	T7562	T7561	aux	to,expressed
R4317	T7563	T7561	auxpass	be,expressed
R4318	T7564	T7561	advmod	widely,expressed
R4319	T7565	T7566	punct	[,38
R432	T1156	T1149	conj	lost,gained
R4320	T7566	T7543	parataxis	38,observed
R4321	T7567	T7566	punct	],38
R4322	T7568	T7543	punct	.,observed
R4323	T7570	T7571	nsubj	We,observe
R4324	T7572	T7573	compound	Sam68,staining
R4325	T7573	T7571	dobj	staining,observe
R4326	T7574	T7571	prep	in,observe
R4327	T7575	T7576	det	the,brain
R4328	T7576	T7574	pobj	brain,in
R4329	T7577	T7576	punct	", ",brain
R433	T1157	T1156	prep	during,lost
R4330	T7578	T7576	conj	heart,brain
R4331	T7579	T7578	punct	", ",heart
R4332	T7580	T7578	cc	and,heart
R4333	T7581	T7578	conj	intestine,heart
R4334	T7582	T7583	punct	(,see
R4335	T7583	T7581	parataxis	see,intestine
R4336	T7584	T7583	dobj	Figure,see
R4337	T7585	T7584	nummod	1,Figure
R4338	T7586	T7583	punct	),see
R4339	T7587	T7588	advmod	as,as
R434	T1158	T1157	pobj	aging,during
R4340	T7588	T7576	cc	as,brain
R4341	T7589	T7588	advmod	well,as
R4342	T7590	T7576	conj	liver,brain
R4343	T7591	T7590	punct	", ",liver
R4344	T7592	T7590	conj	skin,liver
R4345	T7593	T7592	punct	", ",skin
R4346	T7594	T7592	cc	and,skin
R4347	T7595	T7592	conj	kidney,skin
R4348	T7596	T7597	punct	(,data
R4349	T7597	T7595	meta	data,kidney
R435	T1159	T1145	auxpass	are,determined
R4350	T7598	T7597	amod	unpublished,data
R4351	T7599	T7597	punct	),data
R4352	T7600	T7576	prep	of,brain
R4353	T7601	T7602	amod	late,stage
R4354	T7602	T7604	compound	stage,embryos
R4355	T7603	T7602	punct	-,stage
R4356	T7604	T7600	pobj	embryos,of
R4357	T7605	T7571	punct	.,observe
R4358	T7607	T7608	det	This,pattern
R4359	T7608	T7610	nsubj	pattern,is
R436	T1160	T1145	prep	in,determined
R4360	T7609	T7608	compound	expression,pattern
R4361	T7611	T7610	acomp	consistent,is
R4362	T7612	T7611	prep	with,consistent
R4363	T7613	T7614	amod	previous,work
R4364	T7614	T7612	pobj	work,with
R4365	T7615	T7616	dep	that,observed
R4366	T7616	T7614	relcl	observed,work
R4367	T7617	T7616	aux	has,observed
R4368	T7618	T7616	dobj	Sam68,observed
R4369	T7619	T7618	prep	in,Sam68
R437	T1161	T1162	amod	large,part
R4370	T7620	T7619	pobj	neurons,in
R4371	T7621	T7622	punct	[,52
R4372	T7622	T7619	parataxis	52,in
R4373	T7623	T7622	punct	],52
R4374	T7624	T7619	cc	and,in
R4375	T7625	T7619	conj	as,in
R4376	T7626	T7627	det	a,substrate
R4377	T7627	T7625	pobj	substrate,as
R4378	T7628	T7627	prep	of,substrate
R4379	T7629	T7630	det	the,kinase
R438	T1162	T1160	pobj	part,in
R4380	T7630	T7628	pobj	kinase,of
R4381	T7631	T7632	amod	intestinal,SIK
R4382	T7632	T7630	nmod	SIK,kinase
R4383	T7633	T7632	punct	/,SIK
R4384	T7634	T7635	compound	breast,tumor
R4385	T7635	T7632	appos	tumor,SIK
R4386	T7636	T7637	punct	[,43
R4387	T7637	T7627	parataxis	43,substrate
R4388	T7638	T7637	punct	],43
R4389	T7639	T7610	punct	.,is
R439	T1163	T1145	prep	by,determined
R4390	T7641	T7642	det	The,expression
R4391	T7642	T7643	nsubjpass	expression,altered
R4392	T7644	T7642	prep	of,expression
R4393	T7645	T7644	pobj	Sam68,of
R4394	T7646	T7643	auxpass	was,altered
R4395	T7647	T7643	neg	not,altered
R4396	T7648	T7643	prep	in,altered
R4397	T7649	T7650	amod	aging,cells
R4398	T7650	T7648	pobj	cells,in
R4399	T7651	T7652	nmod	bone,marrow
R44	T281	T282	amod	several,aspects
R440	T1164	T1163	pobj	genetics,by
R4400	T7652	T7650	nmod	marrow,cells
R4401	T7653	T7650	amod	stromal,cells
R4402	T7654	T7648	cc	or,in
R4403	T7655	T7648	conj	in,in
R4404	T7656	T7657	amod	senescencing,cells
R4405	T7657	T7655	pobj	cells,in
R4406	T7658	T7657	nmod	WI,cells
R4407	T7659	T7658	punct	-,WI
R4408	T7660	T7658	nummod	38,WI
R4409	T7661	T7662	punct	(,data
R441	T1165	T1166	punct	[,4
R4410	T7662	T7643	meta	data,altered
R4411	T7663	T7662	amod	unpublished,data
R4412	T7664	T7662	punct	),data
R4413	T7665	T7643	punct	.,altered
R4414	T7667	T7668	det	The,presence
R4415	T7668	T7669	nsubj	presence,predicted
R4416	T7670	T7668	prep	of,presence
R4417	T7671	T7670	pobj	Sam68,of
R4418	T7672	T7668	prep	in,presence
R4419	T7673	T7672	pobj	chondrocytes,in
R442	T1166	T1164	parataxis	4,genetics
R4420	T7674	T7673	punct	", ",chondrocytes
R4421	T7675	T7673	conj	osteoblasts,chondrocytes
R4422	T7676	T7675	cc	and,osteoblasts
R4423	T7677	T7675	conj	osteoclasts,osteoblasts
R4424	T7678	T7673	prep	in,chondrocytes
R4425	T7679	T7680	amod	developing,cartilage
R4426	T7680	T7678	pobj	cartilage,in
R4427	T7681	T7680	cc	and,cartilage
R4428	T7682	T7680	conj	bone,cartilage
R4429	T7683	T7684	det	a,role
R443	T1167	T1168	punct	–,6
R4430	T7684	T7669	dobj	role,predicted
R4431	T7685	T7684	amod	pivotal,role
R4432	T7686	T7684	prep	for,role
R4433	T7687	T7688	det	the,protein
R4434	T7688	T7686	pobj	protein,for
R4435	T7689	T7684	prep	in,role
R4436	T7690	T7691	amod	skeletal,development
R4437	T7691	T7689	pobj	development,in
R4438	T7692	T7669	punct	.,predicted
R4439	T7694	T7695	det	The,mice
R444	T1168	T1166	prep	6,4
R4440	T7695	T7700	nsubjpass	mice,generated
R4441	T7696	T7695	nmod	Sam68,mice
R4442	T7697	T7696	punct	−,Sam68
R4443	T7698	T7696	punct	/,Sam68
R4444	T7699	T7696	punct	−,Sam68
R4445	T7701	T7700	auxpass	were,generated
R4446	T7702	T7700	advcl	using,generated
R4447	T7703	T7704	det	a,approach
R4448	T7704	T7702	dobj	approach,using
R4449	T7705	T7704	amod	traditional,approach
R445	T1169	T1166	punct	],4
R4450	T7706	T7704	compound	targeting,approach
R4451	T7707	T7708	advmod	where,deleted
R4452	T7708	T7704	relcl	deleted,approach
R4453	T7709	T7710	det	the,domain
R4454	T7710	T7708	nsubjpass	domain,deleted
R4455	T7711	T7710	amod	functional,domain
R4456	T7712	T7710	compound	KH,domain
R4457	T7713	T7708	auxpass	was,deleted
R4458	T7714	T7700	cc	and,generated
R4459	T7715	T7716	advmod	approximately,one
R446	T1170	T1163	cc	but,by
R4460	T7716	T7717	nsubj	one,survived
R4461	T7717	T7700	conj	survived,generated
R4462	T7718	T7716	amod	third,one
R4463	T7719	T7716	prep	of,one
R4464	T7720	T7721	det	the,mice
R4465	T7721	T7719	pobj	mice,of
R4466	T7722	T7721	nmod	Sam68,mice
R4467	T7723	T7722	punct	−,Sam68
R4468	T7724	T7722	punct	/,Sam68
R4469	T7725	T7722	punct	−,Sam68
R447	T1171	T1170	advmod	also,but
R4470	T7726	T7717	prep	to,survived
R4471	T7727	T7726	pobj	adulthood,to
R4472	T7728	T7717	prep	with,survived
R4473	T7729	T7730	det	no,defects
R4474	T7730	T7728	pobj	defects,with
R4475	T7731	T7730	amod	apparent,defects
R4476	T7732	T7717	punct	.,survived
R4477	T7734	T7735	nummod	One,explanation
R4478	T7735	T7736	nsubj	explanation,is
R4479	T7737	T7735	prep	for,explanation
R448	T1172	T1163	conj	by,by
R4480	T7738	T7739	det	this,phenomenon
R4481	T7739	T7737	pobj	phenomenon,for
R4482	T7740	T7741	mark	that,sub-served
R4483	T7741	T7736	ccomp	sub-served,is
R4484	T7742	T7743	compound	Sam68,function
R4485	T7743	T7741	nsubjpass	function,sub-served
R4486	T7744	T7741	auxpass	is,sub-served
R4487	T7745	T7741	agent	by,sub-served
R4488	T7746	T7745	pobj	one,by
R4489	T7747	T7746	cc	or,one
R449	T1173	T1174	amod	physical,activity
R4490	T7748	T7746	conj	more,one
R4491	T7749	T7746	prep	of,one
R4492	T7750	T7751	poss	its,members
R4493	T7751	T7749	pobj	members,of
R4494	T7752	T7751	compound	family,members
R4495	T7753	T7741	prep	during,sub-served
R4496	T7754	T7753	pobj	development,during
R4497	T7755	T7756	amod	such,as
R4498	T7756	T7741	prep	as,sub-served
R4499	T7757	T7756	pobj	SLM,as
R45	T282	T280	dobj	aspects,regulate
R450	T1174	T1172	pobj	activity,by
R4500	T7758	T7757	punct	-,SLM
R4501	T7759	T7757	nummod	1,SLM
R4502	T7760	T7757	cc	and,SLM
R4503	T7761	T7757	conj	SLM,SLM
R4504	T7762	T7761	punct	-,SLM
R4505	T7763	T7761	nummod	2,SLM
R4506	T7764	T7765	punct	[,47
R4507	T7765	T7736	parataxis	47,is
R4508	T7766	T7765	punct	],47
R4509	T7767	T7736	punct	.,is
R451	T1175	T1172	cc	and,by
R4510	T7769	T7770	advmod	Alternatively,required
R4511	T7771	T7770	punct	", ",required
R4512	T7772	T7770	nsubjpass	Sam68,required
R4513	T7773	T7770	auxpass	is,required
R4514	T7774	T7770	neg	not,required
R4515	T7775	T7770	prep	during,required
R4516	T7776	T7777	amod	embryonic,development
R4517	T7777	T7775	pobj	development,during
R4518	T7778	T7770	punct	.,required
R4519	T7780	T7781	advmod	However,killed
R452	T1176	T1172	conj	by,by
R4520	T7782	T7781	punct	", ",killed
R4521	T7783	T7784	nummod	two,thirds
R4522	T7784	T7781	nsubjpass	thirds,killed
R4523	T7785	T7784	prep	of,thirds
R4524	T7786	T7787	det	the,Sam68
R4525	T7787	T7785	pobj	Sam68,of
R4526	T7788	T7787	punct	−,Sam68
R4527	T7789	T7787	punct	/,Sam68
R4528	T7790	T7787	punct	−,Sam68
R4529	T7791	T7787	punct	", ",Sam68
R453	T1177	T1176	pobj	alterations,by
R4530	T7792	T7787	cc	but,Sam68
R4531	T7793	T7792	neg	not,but
R4532	T7794	T7795	det	the,Sam68
R4533	T7795	T7796	nmod	Sam68,pups
R4534	T7796	T7787	conj	pups,Sam68
R4535	T7797	T7795	punct	+,Sam68
R4536	T7798	T7795	punct	/,Sam68
R4537	T7799	T7795	punct	-,Sam68
R4538	T7800	T7781	punct	", ",killed
R4539	T7801	T7781	auxpass	were,killed
R454	T1178	T1177	prep	in,alterations
R4540	T7802	T7781	agent	by,killed
R4541	T7803	T7804	poss	their,mothers
R4542	T7804	T7802	pobj	mothers,by
R4543	T7805	T7804	nmod	Sam68,mothers
R4544	T7806	T7805	punct	+,Sam68
R4545	T7807	T7805	punct	/,Sam68
R4546	T7808	T7805	punct	-,Sam68
R4547	T7809	T7781	punct	.,killed
R4548	T7811	T7812	det	These,findings
R4549	T7812	T7813	nsubj	findings,suggest
R455	T1179	T1180	det	the,availability
R4550	T7814	T7815	mark	that,are
R4551	T7815	T7813	ccomp	are,suggest
R4552	T7816	T7817	det	the,mothers
R4553	T7817	T7815	nsubj	mothers,are
R4554	T7818	T7815	acomp	able,are
R4555	T7819	T7820	aux	to,detect
R4556	T7820	T7818	xcomp	detect,able
R4557	T7821	T7822	det	a,difference
R4558	T7822	T7820	dobj	difference,detect
R4559	T7823	T7822	amod	subtle,difference
R456	T1180	T1178	pobj	availability,in
R4560	T7824	T7822	compound	defect,difference
R4561	T7825	T7822	punct	/,difference
R4562	T7826	T7827	dep	that,can
R4563	T7827	T7822	relcl	can,difference
R4564	T7828	T7827	nsubj	we,can
R4565	T7829	T7827	neg	not,can
R4566	T7830	T7813	punct	.,suggest
R4567	T7832	T7833	amod	Adult,mice
R4568	T7833	T7838	nsubj	mice,live
R4569	T7834	T7833	nmod	Sam68,mice
R457	T1181	T1180	cc	and,availability
R4570	T7835	T7834	punct	−,Sam68
R4571	T7836	T7834	punct	/,Sam68
R4572	T7837	T7834	punct	−,Sam68
R4573	T7839	T7840	det	a,span
R4574	T7840	T7838	dobj	span,live
R4575	T7841	T7840	amod	normal,span
R4576	T7842	T7840	compound	life,span
R4577	T7843	T7840	prep	of,span
R4578	T7844	T7845	advmod	approximately,two
R4579	T7845	T7846	nummod	two,years
R458	T1182	T1180	conj	response,availability
R4580	T7846	T7843	pobj	years,of
R4581	T7847	T7838	punct	.,live
R4582	T7849	T7850	det	The,males
R4583	T7850	T7855	nsubj	males,were
R4584	T7851	T7850	nmod	Sam68,males
R4585	T7852	T7851	punct	−,Sam68
R4586	T7853	T7851	punct	/,Sam68
R4587	T7854	T7851	punct	−,Sam68
R4588	T7856	T7855	acomp	sterile,were
R4589	T7857	T7855	cc	and,were
R459	T1183	T1180	prep	of,availability
R4590	T7858	T7859	det	the,females
R4591	T7859	T7864	nsubj	females,provided
R4592	T7860	T7859	nmod	Sam68,females
R4593	T7861	T7860	punct	−,Sam68
R4594	T7862	T7860	punct	/,Sam68
R4595	T7863	T7860	punct	−,Sam68
R4596	T7864	T7855	conj	provided,were
R4597	T7865	T7866	amod	inadequate,care
R4598	T7866	T7864	dobj	care,provided
R4599	T7867	T7864	dative	to,provided
R46	T283	T282	prep	of,aspects
R460	T1184	T1185	compound	bone,cells
R4600	T7868	T7869	poss	their,young
R4601	T7869	T7867	pobj	young,to
R4602	T7870	T7855	punct	", ",were
R4603	T7871	T7855	cc	but,were
R4604	T7872	T7873	det	the,pups
R4605	T7873	T7874	nsubjpass	pups,scattered
R4606	T7874	T7855	conj	scattered,were
R4607	T7875	T7874	auxpass	were,scattered
R4608	T7876	T7874	neg	not,scattered
R4609	T7877	T7874	cc	and,scattered
R461	T1185	T1183	pobj	cells,of
R4610	T7878	T7874	conj	neglected,scattered
R4611	T7879	T7880	mark	as,observed
R4612	T7880	T7878	advcl	observed,neglected
R4613	T7881	T7880	prep	with,observed
R4614	T7882	T7883	nmod	FosB,mice
R4615	T7883	T7881	pobj	mice,with
R4616	T7884	T7882	punct	−,FosB
R4617	T7885	T7882	punct	/,FosB
R4618	T7886	T7882	punct	−,FosB
R4619	T7887	T7888	punct	[,53
R462	T1186	T1180	prep	to,availability
R4620	T7888	T7878	parataxis	53,neglected
R4621	T7889	T7888	punct	],53
R4622	T7890	T7874	punct	.,scattered
R4623	T7892	T7893	compound	Serum,levels
R4624	T7893	T7894	nsubj	levels,decreased
R4625	T7894	T7897	ccomp	decreased,decreased
R4626	T7895	T7893	prep	of,levels
R4627	T7896	T7895	pobj	estrogen,of
R4628	T7898	T7894	prep	in,decreased
R4629	T7899	T7900	amod	aging,females
R463	T1187	T1188	amod	circulating,hormones
R4630	T7900	T7898	pobj	females,in
R4631	T7901	T7900	nmod	Sam68,females
R4632	T7902	T7901	punct	−,Sam68
R4633	T7903	T7901	punct	/,Sam68
R4634	T7904	T7901	punct	−,Sam68
R4635	T7905	T7906	mark	as,expected
R4636	T7906	T7894	advcl	expected,decreased
R4637	T7907	T7897	punct	;,decreased
R4638	T7908	T7897	advmod	however,decreased
R4639	T7909	T7897	punct	", ",decreased
R464	T1188	T1186	pobj	hormones,to
R4640	T7910	T7911	det	the,levels
R4641	T7911	T7897	nsubj	levels,decreased
R4642	T7912	T7911	compound	leptin,levels
R4643	T7913	T7897	prep	in,decreased
R4644	T7914	T7915	amod	aged,females
R4645	T7915	T7913	pobj	females,in
R4646	T7916	T7915	nmod	Sam68,females
R4647	T7917	T7916	punct	−,Sam68
R4648	T7918	T7916	punct	/,Sam68
R4649	T7919	T7916	punct	−,Sam68
R465	T1189	T1190	punct	[,7
R4650	T7920	T7897	punct	.,decreased
R4651	T7922	T7923	det	The,females
R4652	T7923	T7929	nsubj	females,were
R4653	T7924	T7923	amod	aged,females
R4654	T7925	T7923	nmod	Sam68,females
R4655	T7926	T7925	punct	−,Sam68
R4656	T7927	T7925	punct	/,Sam68
R4657	T7928	T7925	punct	−,Sam68
R4658	T7930	T7929	neg	not,were
R4659	T7931	T7929	acomp	obese,were
R466	T1190	T1188	parataxis	7,hormones
R4660	T7932	T7929	cc	and,were
R4661	T7933	T7934	advmod	actually,weighed
R4662	T7934	T7929	conj	weighed,were
R4663	T7935	T7934	dobj	less,weighed
R4664	T7936	T7935	prep	than,less
R4665	T7937	T7938	det	the,controls
R4666	T7938	T7936	pobj	controls,than
R4667	T7939	T7938	compound	littermate,controls
R4668	T7940	T7941	punct	(,see
R4669	T7941	T7934	parataxis	see,weighed
R467	T1191	T1192	punct	–,9
R4670	T7942	T7941	dobj	Table,see
R4671	T7943	T7942	nummod	2,Table
R4672	T7944	T7941	punct	),see
R4673	T7945	T7929	punct	.,were
R4674	T7947	T7948	advmod	Moreover,altered
R4675	T7949	T7948	punct	", ",altered
R4676	T7950	T7951	poss	their,appetite
R4677	T7951	T7948	nsubjpass	appetite,altered
R4678	T7952	T7948	auxpass	was,altered
R4679	T7953	T7948	neg	not,altered
R468	T1192	T1190	prep	9,7
R4680	T7954	T7948	prep	with,altered
R4681	T7955	T7954	pobj	aging,with
R4682	T7956	T7957	punct	(,N.
R4683	T7957	T7948	meta	N.,altered
R4684	T7958	T7957	nmod	Torabi,N.
R4685	T7959	T7957	cc	and,N.
R4686	T7960	T7957	conj	S.,N.
R4687	T7961	T7960	nmod	Richard,S.
R4688	T7962	T7960	punct	", ",S.
R4689	T7963	T7964	amod	unpublished,data
R469	T1193	T1190	punct	],7
R4690	T7964	T7960	conj	data,S.
R4691	T7965	T7964	punct	),data
R4692	T7966	T7948	punct	", ",altered
R4693	T7967	T7948	advcl	suggesting,altered
R4694	T7968	T7969	mark	that,recreating
R4695	T7969	T7967	ccomp	recreating,suggesting
R4696	T7970	T7971	det	the,reduction
R4697	T7971	T7969	nsubj	reduction,recreating
R4698	T7972	T7971	amod	observed,reduction
R4699	T7973	T7971	compound	leptin,reduction
R47	T284	T285	compound	RNA,metabolism
R470	T1194	T1188	cc	and,hormones
R4700	T7974	T7969	aux	was,recreating
R4701	T7975	T7969	neg	not,recreating
R4702	T7976	T7977	dep	ob,like
R4703	T7977	T7981	amod	like,phenotypes
R4704	T7978	T7977	punct	/,like
R4705	T7979	T7977	npadvmod	ob,like
R4706	T7980	T7977	punct	-,like
R4707	T7981	T7969	dobj	phenotypes,recreating
R4708	T7982	T7981	acl	related,phenotypes
R4709	T7983	T7982	prep	to,related
R471	T1195	T1196	advmod	locally,derived
R4710	T7984	T7983	pobj	weight,to
R4711	T7985	T7984	punct	", ",weight
R4712	T7986	T7984	conj	appetite,weight
R4713	T7987	T7986	cc	and,appetite
R4714	T7988	T7989	amod	female,sterility
R4715	T7989	T7986	conj	sterility,appetite
R4716	T7990	T7991	punct	[,54
R4717	T7991	T7948	parataxis	54,altered
R4718	T7992	T7991	punct	],54
R4719	T7993	T7948	punct	.,altered
R472	T1196	T1197	amod	derived,factors
R4720	T7995	T7996	det	The,phenotyping
R4721	T7996	T7998	nsubj	phenotyping,showed
R4722	T7997	T7996	amod	skeletal,phenotyping
R4723	T7999	T7996	prep	of,phenotyping
R4724	T8000	T7999	pobj	cohorts,of
R4725	T8001	T8000	prep	of,cohorts
R4726	T8002	T8003	nmod	Sam68,mice
R4727	T8003	T8001	pobj	mice,of
R4728	T8004	T8002	punct	+,Sam68
R4729	T8005	T8002	punct	/,Sam68
R473	T1197	T1188	conj	factors,hormones
R4730	T8006	T8002	punct	+,Sam68
R4731	T8007	T8002	cc	and,Sam68
R4732	T8008	T8002	conj	Sam68,Sam68
R4733	T8009	T8008	punct	−,Sam68
R4734	T8010	T8008	punct	/,Sam68
R4735	T8011	T8008	punct	−,Sam68
R4736	T8012	T8013	mark	that,preserved
R4737	T8013	T7998	ccomp	preserved,showed
R4738	T8014	T8015	compound	bone,mass
R4739	T8015	T8013	nsubjpass	mass,preserved
R474	T1198	T1197	compound	growth,factors
R4740	T8016	T8013	auxpass	was,preserved
R4741	T8017	T8013	prep	in,preserved
R4742	T8018	T8019	amod	aged,mice
R4743	T8019	T8017	pobj	mice,in
R4744	T8020	T8019	nmod	Sam68,mice
R4745	T8021	T8020	punct	−,Sam68
R4746	T8022	T8020	punct	/,Sam68
R4747	T8023	T8020	punct	−,Sam68
R4748	T8024	T7998	punct	.,showed
R4749	T8026	T8027	amod	Traditional,histology
R475	T1199	T1200	punct	[,11
R4750	T8027	T8028	nsubj	histology,suggested
R4751	T8029	T8027	cc	and,histology
R4752	T8030	T8027	conj	histomorphometry,histology
R4753	T8031	T8032	mark	that,involved
R4754	T8032	T8028	ccomp	involved,suggested
R4755	T8033	T8034	det	the,mechanism
R4756	T8034	T8032	nsubj	mechanism,involved
R4757	T8035	T8032	dobj	preservation,involved
R4758	T8036	T8035	prep	of,preservation
R4759	T8037	T8038	nmod	osteoblast,activity
R476	T1200	T1197	parataxis	11,factors
R4760	T8038	T8036	pobj	activity,of
R4761	T8039	T8037	cc	and,osteoblast
R4762	T8040	T8037	conj	osteoclast,osteoblast
R4763	T8041	T8028	dep	.,suggested
R4764	T8043	T8044	det	The,role
R4765	T8044	T8046	nsubj	role,led
R4766	T8045	T8044	amod	documented,role
R4767	T8047	T8044	prep	of,role
R4768	T8048	T8049	det	the,protein
R4769	T8049	T8047	pobj	protein,of
R477	T1201	T1200	nummod	10,11
R4770	T8050	T8049	nmod	Sam68,protein
R4771	T8051	T8049	amod	regulatory,protein
R4772	T8052	T8049	punct	", ",protein
R4773	T8053	T8049	appos	Src,protein
R4774	T8054	T8044	punct	", ",role
R4775	T8055	T8044	prep	in,role
R4776	T8056	T8055	pobj	osteopetrosis,in
R4777	T8057	T8046	dobj	us,led
R4778	T8058	T8059	aux	to,investigate
R4779	T8059	T8046	xcomp	investigate,led
R478	T1202	T1200	punct	",",11
R4780	T8060	T8061	det	the,morphology
R4781	T8061	T8059	dobj	morphology,investigate
R4782	T8062	T8061	cc	and,morphology
R4783	T8063	T8061	conj	activity,morphology
R4784	T8064	T8061	prep	of,morphology
R4785	T8065	T8066	nmod	Sam68,osteoclasts
R4786	T8066	T8064	pobj	osteoclasts,of
R4787	T8067	T8065	punct	−,Sam68
R4788	T8068	T8065	punct	/,Sam68
R4789	T8069	T8065	punct	−,Sam68
R479	T1203	T1200	punct	],11
R4790	T8070	T8071	advmod	ex,vivo
R4791	T8071	T8059	advmod	vivo,investigate
R4792	T8072	T8046	punct	.,led
R4793	T8074	T8075	det	The,kinase
R4794	T8075	T8078	nsubjpass	kinase,shown
R4795	T8076	T8075	compound	Src,kinase
R4796	T8077	T8075	compound	tyrosine,kinase
R4797	T8079	T8078	auxpass	was,shown
R4798	T8080	T8081	aux	to,play
R4799	T8081	T8078	xcomp	play,shown
R48	T285	T283	pobj	metabolism,of
R480	T1204	T1145	punct	.,determined
R4800	T8082	T8083	det	a,role
R4801	T8083	T8081	dobj	role,play
R4802	T8084	T8081	prep	in,play
R4803	T8085	T8086	compound	bone,remodeling
R4804	T8086	T8084	pobj	remodeling,in
R4805	T8087	T8088	advmod	when,died
R4806	T8088	T8081	advcl	died,play
R4807	T8089	T8090	nmod	Src,mice
R4808	T8090	T8088	nsubj	mice,died
R4809	T8091	T8089	punct	−,Src
R481	T1206	T1207	mark	Whereas,provided
R4810	T8092	T8089	punct	/,Src
R4811	T8093	T8089	punct	−,Src
R4812	T8094	T8088	prep	at,died
R4813	T8095	T8096	nummod	6,months
R4814	T8096	T8094	pobj	months,at
R4815	T8097	T8096	prep	of,months
R4816	T8098	T8097	pobj	age,of
R4817	T8099	T8088	prep	with,died
R4818	T8100	T8101	det	an,phenotype
R4819	T8101	T8099	pobj	phenotype,with
R482	T1207	T1213	advcl	provided,known
R4820	T8102	T8101	amod	osteopetrotic,phenotype
R4821	T8103	T8104	punct	[,48
R4822	T8104	T8088	parataxis	48,died
R4823	T8105	T8104	punct	],48
R4824	T8106	T8088	cc	and,died
R4825	T8107	T8108	det	the,defect
R4826	T8108	T8109	nsubjpass	defect,attributed
R4827	T8109	T8088	conj	attributed,died
R4828	T8110	T8109	auxpass	was,attributed
R4829	T8111	T8109	prep	to,attributed
R483	T1208	T1209	amod	genetic,based
R4830	T8112	T8113	amod	defective,function
R4831	T8113	T8111	pobj	function,to
R4832	T8114	T8113	compound	osteoclast,function
R4833	T8115	T8116	punct	[,55
R4834	T8116	T8109	parataxis	55,attributed
R4835	T8117	T8118	punct	−,59
R4836	T8118	T8116	prep	59,55
R4837	T8119	T8116	punct	],55
R4838	T8120	T8078	punct	.,shown
R4839	T8122	T8123	nsubj	We,cultured
R484	T1209	T1211	amod	based,studies
R4840	T8124	T8123	advmod	therefore,cultured
R4841	T8125	T8126	amod	mature,osteoclasts
R4842	T8126	T8123	dobj	osteoclasts,cultured
R4843	T8127	T8126	acl	harvested,osteoclasts
R4844	T8128	T8127	prep	from,harvested
R4845	T8129	T8130	nmod	Sam68,mice
R4846	T8130	T8128	pobj	mice,from
R4847	T8131	T8129	punct	+,Sam68
R4848	T8132	T8129	punct	/,Sam68
R4849	T8133	T8129	punct	+,Sam68
R485	T1210	T1209	punct	-,based
R4850	T8134	T8129	cc	and,Sam68
R4851	T8135	T8129	conj	Sam68,Sam68
R4852	T8136	T8135	punct	−,Sam68
R4853	T8137	T8135	punct	/,Sam68
R4854	T8138	T8135	punct	−,Sam68
R4855	T8139	T8140	advmod	ex,vivo
R4856	T8140	T8123	advmod	vivo,cultured
R4857	T8141	T8123	prep	on,cultured
R4858	T8142	T8143	compound	dentin,slices
R4859	T8143	T8141	pobj	slices,on
R486	T1211	T1207	nsubj	studies,provided
R4860	T8144	T8145	aux	to,quantify
R4861	T8145	T8123	advcl	quantify,cultured
R4862	T8146	T8147	poss	their,capacity
R4863	T8147	T8145	dobj	capacity,quantify
R4864	T8148	T8147	amod	resorptive,capacity
R4865	T8149	T8123	punct	.,cultured
R4866	T8151	T8152	det	The,fact
R4867	T8152	T8153	nsubj	fact,made
R4868	T8154	T8155	mark	that,looked
R4869	T8155	T8152	acl	looked,fact
R487	T1212	T1207	aux	have,provided
R4870	T8156	T8157	nmod	Sam68,osteoclasts
R4871	T8157	T8155	nsubj	osteoclasts,looked
R4872	T8158	T8156	punct	−,Sam68
R4873	T8159	T8156	punct	/,Sam68
R4874	T8160	T8156	punct	−,Sam68
R4875	T8161	T8155	cc	and,looked
R4876	T8162	T8155	conj	acted,looked
R4877	T8163	T8162	prep	like,acted
R4878	T8164	T8165	nmod	Sam68,osteoclasts
R4879	T8165	T8163	pobj	osteoclasts,like
R488	T1214	T1215	amod	novel,insights
R4880	T8166	T8164	punct	+,Sam68
R4881	T8167	T8164	punct	/,Sam68
R4882	T8168	T8164	punct	+,Sam68
R4883	T8169	T8170	advmod	ex,vivo
R4884	T8170	T8162	advmod	vivo,acted
R4885	T8171	T8170	cc	and,vivo
R4886	T8172	T8173	advmod	in,vivo
R4887	T8173	T8170	conj	vivo,vivo
R4888	T8174	T8175	nsubj	it,unlikely
R4889	T8175	T8153	ccomp	unlikely,made
R489	T1215	T1207	dobj	insights,provided
R4890	T8176	T8177	mark	that,was
R4891	T8177	T8175	advcl	was,unlikely
R4892	T8178	T8177	nsubj	this,was
R4893	T8179	T8180	det	the,source
R4894	T8180	T8177	attr	source,was
R4895	T8181	T8180	amod	primary,source
R4896	T8182	T8180	prep	of,source
R4897	T8183	T8184	det	the,difference
R4898	T8184	T8182	pobj	difference,of
R4899	T8185	T8184	prep	in,difference
R49	T286	T267	punct	;,remained
R490	T1216	T1215	prep	into,insights
R4900	T8186	T8187	compound	bone,metabolism
R4901	T8187	T8185	pobj	metabolism,in
R4902	T8188	T8177	prep	in,was
R4903	T8189	T8190	nummod	12,month
R4904	T8190	T8192	npadvmod	month,old
R4905	T8191	T8190	punct	-,month
R4906	T8192	T8194	amod	old,mice
R4907	T8193	T8192	punct	-,old
R4908	T8194	T8188	pobj	mice,in
R4909	T8195	T8194	nmod	Sam68,mice
R491	T1217	T1218	det	the,pathways
R4910	T8196	T8195	punct	−,Sam68
R4911	T8197	T8195	punct	/,Sam68
R4912	T8198	T8195	punct	−,Sam68
R4913	T8199	T8153	punct	.,made
R4914	T8201	T8202	det	The,fact
R4915	T8202	T8203	nsubj	fact,suggested
R4916	T8204	T8205	mark	that,were
R4917	T8205	T8202	acl	were,fact
R4918	T8206	T8207	det	the,levels
R4919	T8207	T8205	nsubj	levels,were
R492	T1218	T1216	pobj	pathways,into
R4920	T8208	T8207	compound	CTX,levels
R4921	T8209	T8205	acomp	lower,were
R4922	T8210	T8205	prep	in,were
R4923	T8211	T8212	amod	young,mice
R4924	T8212	T8210	pobj	mice,in
R4925	T8213	T8211	cc	and,young
R4926	T8214	T8211	conj	old,young
R4927	T8215	T8212	nmod	Sam68,mice
R4928	T8216	T8215	punct	−,Sam68
R4929	T8217	T8215	punct	/,Sam68
R493	T1219	T1220	dep	that,regulate
R4930	T8218	T8215	punct	−,Sam68
R4931	T8219	T8220	mark	that,is
R4932	T8220	T8203	ccomp	is,suggested
R4933	T8221	T8220	expl	there,is
R4934	T8222	T8223	amod	reduced,resorption
R4935	T8223	T8220	attr	resorption,is
R4936	T8224	T8223	compound	bone,resorption
R4937	T8225	T8223	prep	compared,resorption
R4938	T8226	T8225	prep	with,compared
R4939	T8227	T8228	amod	wild,type
R494	T1220	T1218	relcl	regulate,pathways
R4940	T8228	T8230	compound	type,controls
R4941	T8229	T8228	punct	-,type
R4942	T8230	T8226	pobj	controls,with
R4943	T8231	T8230	compound	littermate,controls
R4944	T8232	T8203	punct	.,suggested
R4945	T8234	T8235	advmod	However,occurred
R4946	T8236	T8235	punct	", ",occurred
R4947	T8237	T8238	det	this,reduction
R4948	T8238	T8235	nsubj	reduction,occurred
R4949	T8239	T8238	prep	in,reduction
R495	T1221	T1222	compound	bone,development
R4950	T8240	T8241	compound	bone,resorption
R4951	T8241	T8239	pobj	resorption,in
R4952	T8242	T8235	prep	with,occurred
R4953	T8243	T8244	amod	normal,activity
R4954	T8244	T8242	pobj	activity,with
R4955	T8245	T8244	compound	osteoclast,activity
R4956	T8246	T8235	punct	", ",occurred
R4957	T8247	T8248	mark	as,assessed
R4958	T8248	T8235	advcl	assessed,occurred
R4959	T8249	T8248	prep	by,assessed
R496	T1222	T1220	dobj	development,regulate
R4960	T8250	T8251	advmod	in,vitro
R4961	T8251	T8252	amod	vitro,culturing
R4962	T8252	T8249	pobj	culturing,by
R4963	T8253	T8235	punct	.,occurred
R4964	T8255	T8256	det	These,observations
R4965	T8256	T8257	nsubj	observations,are
R4966	T8258	T8257	acomp	consistent,are
R4967	T8259	T8258	prep	with,consistent
R4968	T8260	T8261	det	the,mice
R4969	T8261	T8266	nsubj	mice,having
R497	T1223	T1224	punct	[,12
R4970	T8262	T8261	nmod	Sam68,mice
R4971	T8263	T8262	punct	−,Sam68
R4972	T8264	T8262	punct	/,Sam68
R4973	T8265	T8262	punct	−,Sam68
R4974	T8266	T8259	pcomp	having,with
R4975	T8267	T8268	det	a,phenotype
R4976	T8268	T8266	dobj	phenotype,having
R4977	T8269	T8270	npadvmod	youth,like
R4978	T8270	T8268	amod	like,phenotype
R4979	T8271	T8270	punct	-,like
R498	T1224	T1220	parataxis	12,regulate
R4980	T8272	T8268	compound	bone,phenotype
R4981	T8273	T8257	punct	.,are
R4982	T8275	T8276	advmod	However,is
R4983	T8277	T8276	punct	", ",is
R4984	T8278	T8276	nsubj	it,is
R4985	T8279	T8276	advmod	still,is
R4986	T8280	T8276	acomp	possible,is
R4987	T8281	T8276	punct	", ",is
R4988	T8282	T8283	mark	that,manifest
R4989	T8283	T8276	ccomp	manifest,is
R499	T1225	T1226	punct	–,14
R4990	T8284	T8285	det	a,impairment
R4991	T8285	T8283	nsubj	impairment,manifest
R4992	T8286	T8285	amod	mild,impairment
R4993	T8287	T8285	prep	in,impairment
R4994	T8288	T8289	nmod	Sam68,mice
R4995	T8289	T8293	compound	mice,function
R4996	T8290	T8288	punct	−,Sam68
R4997	T8291	T8288	punct	/,Sam68
R4998	T8292	T8288	punct	−,Sam68
R4999	T8293	T8287	pobj	function,in
R5	T239	T236	prep	of,Ablation
R50	T287	T267	advmod	however,remained
R500	T1226	T1224	prep	14,12
R5000	T8294	T8293	compound	osteoclast,function
R5001	T8295	T8283	aux	may,manifest
R5002	T8296	T8283	dobj	itself,manifest
R5003	T8297	T8283	advmod	later,manifest
R5004	T8298	T8297	prep	in,later
R5005	T8299	T8298	pobj	life,in
R5006	T8300	T8283	prep	in,manifest
R5007	T8301	T8302	amod	overall,accumulation
R5008	T8302	T8300	pobj	accumulation,in
R5009	T8303	T8302	prep	of,accumulation
R501	T1227	T1224	punct	],12
R5010	T8304	T8303	pobj	bone,of
R5011	T8305	T8276	cc	and,is
R5012	T8306	T8307	nsubj	this,require
R5013	T8307	T8276	conj	require,is
R5014	T8308	T8307	aux	will,require
R5015	T8309	T8310	amod	further,studies
R5016	T8310	T8307	dobj	studies,require
R5017	T8311	T8310	amod	detailed,studies
R5018	T8312	T8276	punct	.,is
R5019	T8314	T8315	advmod	Ex,vivo
R502	T1228	T1213	punct	", ",known
R5020	T8315	T8316	amod	vivo,differentiation
R5021	T8316	T8317	nsubj	differentiation,revealed
R5022	T8318	T8316	prep	of,differentiation
R5023	T8319	T8320	amod	primary,cells
R5024	T8320	T8318	pobj	cells,of
R5025	T8321	T8322	nmod	bone,marrow
R5026	T8322	T8320	nmod	marrow,cells
R5027	T8323	T8320	amod	stromal,cells
R5028	T8324	T8320	punct	", ",cells
R5029	T8325	T8320	acl	harvested,cells
R503	T1229	T1230	advmod	relatively,little
R5030	T8326	T8325	prep	from,harvested
R5031	T8327	T8328	nmod	Sam68,mice
R5032	T8328	T8326	pobj	mice,from
R5033	T8329	T8327	punct	+,Sam68
R5034	T8330	T8327	punct	/,Sam68
R5035	T8331	T8327	punct	+,Sam68
R5036	T8332	T8327	cc	and,Sam68
R5037	T8333	T8327	conj	Sam68,Sam68
R5038	T8334	T8333	punct	−,Sam68
R5039	T8335	T8333	punct	/,Sam68
R504	T1230	T1213	nsubjpass	little,known
R5040	T8336	T8333	punct	−,Sam68
R5041	T8337	T8317	punct	", ",revealed
R5042	T8338	T8317	prep	down,revealed
R5043	T8339	T8340	det	the,lineage
R5044	T8340	T8338	pobj	lineage,down
R5045	T8341	T8340	compound	osteoblast,lineage
R5046	T8342	T8343	det	an,advantage
R5047	T8343	T8317	dobj	advantage,revealed
R5048	T8344	T8343	amod	osteogenic,advantage
R5049	T8345	T8317	prep	in,revealed
R505	T1231	T1213	auxpass	is,known
R5050	T8346	T8347	det	the,cultures
R5051	T8347	T8345	pobj	cultures,in
R5052	T8348	T8347	prep	of,cultures
R5053	T8349	T8348	pobj	cells,of
R5054	T8350	T8349	acl	derived,cells
R5055	T8351	T8350	prep	from,derived
R5056	T8352	T8353	det	the,mice
R5057	T8353	T8351	pobj	mice,from
R5058	T8354	T8353	nmod	Sam68,mice
R5059	T8355	T8354	punct	−,Sam68
R506	T1232	T1213	prep	about,known
R5060	T8356	T8354	punct	/,Sam68
R5061	T8357	T8354	punct	−,Sam68
R5062	T8358	T8317	punct	.,revealed
R5063	T8360	T8361	nsubj	It,be
R5064	T8362	T8361	aux	will,be
R5065	T8363	T8361	acomp	important,be
R5066	T8364	T8365	aux	to,demonstrate
R5067	T8365	T8361	xcomp	demonstrate,be
R5068	T8366	T8367	mark	that,observed
R5069	T8367	T8365	ccomp	observed,demonstrate
R507	T1233	T1234	det	the,etiology
R5070	T8368	T8369	det	a,effect
R5071	T8369	T8367	nsubjpass	effect,observed
R5072	T8370	T8369	amod	similar,effect
R5073	T8407	T8392	prep	into,differentiated
R5074	T8371	T8367	auxpass	is,observed
R5075	T8408	T8407	pobj	osteoblasts,into
R5076	T8372	T8367	advcl	using,observed
R5077	T8409	T8392	prep	with,differentiated
R5078	T8410	T8409	pobj	BMP2,with
R5079	T8411	T8389	punct	.,observed
R508	T1234	T1232	pobj	etiology,about
R5080	T8413	T8414	poss	Our,findings
R5081	T8373	T8374	amod	primary,cells
R5082	T8414	T8415	nsubj	findings,indicate
R5083	T8416	T8417	mark	that,develop
R5084	T8374	T8372	dobj	cells,using
R5085	T8417	T8414	advcl	develop,findings
R5086	T8418	T8419	amod	aged,mice
R5087	T8375	T8376	nmod	bone,marrow
R5088	T8419	T8417	nsubj	mice,develop
R5089	T8420	T8419	nmod	Sam68,mice
R509	T1235	T1234	prep	of,etiology
R5090	T8421	T8420	punct	−,Sam68
R5091	T8376	T8374	nmod	marrow,cells
R5092	T8422	T8420	punct	/,Sam68
R5093	T8423	T8420	punct	−,Sam68
R5094	T8377	T8374	amod	stromal,cells
R5095	T8424	T8417	aux	do,develop
R5096	T8425	T8417	neg	not,develop
R5097	T8426	T8427	amod	fatty,marrow
R5098	T8378	T8374	prep	from,cells
R5099	T8427	T8417	dobj	marrow,develop
R51	T288	T267	punct	", ",remained
R510	T1236	T1237	npadvmod	age,related
R5100	T8428	T8427	compound	bone,marrow
R5101	T8379	T8380	amod	aged,mice
R5102	T8429	T8417	cc	and,develop
R5103	T8430	T8431	mark	that,have
R5104	T8431	T8417	conj	have,develop
R5105	T8380	T8378	pobj	mice,from
R5106	T8432	T8431	nsubj	MEFs,have
R5107	T8433	T8432	acl	derived,MEFs
R5108	T8434	T8433	prep	from,derived
R5109	T8381	T8380	nmod	Sam68,mice
R511	T1237	T1239	amod	related,loss
R5110	T8435	T8436	nmod	Sam68,mice
R5111	T8436	T8434	pobj	mice,from
R5112	T8382	T8381	punct	−,Sam68
R5113	T8437	T8435	punct	−,Sam68
R5114	T8438	T8435	punct	/,Sam68
R5115	T8383	T8381	punct	/,Sam68
R5116	T8439	T8435	punct	−,Sam68
R5117	T8440	T8441	amod	impaired,differentiation
R5118	T8441	T8431	dobj	differentiation,have
R5119	T8442	T8441	compound	adipocyte,differentiation
R512	T1238	T1237	punct	-,related
R5120	T8384	T8381	punct	−,Sam68
R5121	T8443	T8415	punct	", ",indicate
R5122	T8444	T8445	mark	that,regulates
R5123	T8445	T8415	ccomp	regulates,indicate
R5124	T8385	T8361	punct	.,be
R5125	T8446	T8445	nsubj	Sam68,regulates
R5126	T8447	T8448	preconj	both,adipocyte
R5127	T8448	T8449	nmod	adipocyte,differentiation
R5128	T8387	T8388	amod	Similar,findings
R5129	T8449	T8445	dobj	differentiation,regulates
R513	T1239	T1235	pobj	loss,of
R5130	T8450	T8448	cc	and,adipocyte
R5131	T8451	T8448	conj	osteoblast,adipocyte
R5132	T8452	T8415	punct	.,indicate
R5133	T8454	T8455	det	These,findings
R5134	T8455	T8456	nsubj	findings,identify
R5135	T8388	T8389	nsubjpass	findings,observed
R5136	T8390	T8389	auxpass	were,observed
R5137	T8457	T8456	dobj	Sam68,identify
R5138	T8458	T8456	prep	as,identify
R5139	T8391	T8392	advmod	when,differentiated
R514	T1240	T1239	compound	bone,loss
R5140	T8459	T8460	det	the,protein
R5141	T8460	T8458	pobj	protein,as
R5142	T8392	T8389	advcl	differentiated,observed
R5143	T8461	T8460	amod	first,protein
R5144	T8462	T8463	npadvmod	RNA,binding
R5145	T8463	T8460	amod	binding,protein
R5146	T8393	T8394	amod	embryonic,cells
R5147	T8464	T8465	aux	to,regulate
R5148	T8465	T8460	advcl	regulate,protein
R5149	T8394	T8392	nsubjpass	cells,differentiated
R515	T1241	T1213	punct	.,known
R5150	T8466	T8467	amod	mesenchymal,differentiation
R5151	T8467	T8465	dobj	differentiation,regulate
R5152	T8468	T8467	compound	cell,differentiation
R5153	T8395	T8394	amod	mesenchymal,cells
R5154	T8469	T8456	cc	and,identify
R5155	T8470	T8471	det	the,challenge
R5156	T8471	T8472	nsubj	challenge,be
R5157	T8396	T8394	amod	multipotential,cells
R5158	T8472	T8456	conj	be,identify
R5159	T8473	T8472	aux	will,be
R516	T1243	T1244	amod	Increased,resorption
R5160	T8474	T8475	aux	to,identify
R5161	T8397	T8394	compound	progenitor,cells
R5162	T8475	T8472	xcomp	identify,be
R5163	T8476	T8477	det	the,targets
R5164	T8477	T8475	dobj	targets,identify
R5165	T8398	T8394	appos	C3H10T1,cells
R5166	T8478	T8477	amod	specific,targets
R5167	T8479	T8477	compound	RNA,targets
R5168	T8399	T8398	punct	/,C3H10T1
R5169	T8480	T8481	dep	that,regulates
R517	T1244	T1246	nsubjpass	resorption,associated
R5170	T8400	T8398	nummod	2,C3H10T1
R5171	T8481	T8477	relcl	regulates,targets
R5172	T8482	T8481	nsubj	it,regulates
R5173	T8483	T8481	prep	during,regulates
R5174	T8401	T8394	acl	treated,cells
R5175	T8484	T8485	det	this,process
R5176	T8485	T8483	pobj	process,during
R5177	T8486	T8472	punct	.,be
R5178	T8402	T8401	prep	with,treated
R5179	T8488	T8489	det	The,fact
R518	T1245	T1244	compound	bone,resorption
R5180	T8489	T8490	nsubj	fact,raises
R5181	T8403	T8404	compound	Sam68,shRNA
R5182	T8491	T8492	mark	that,altered
R5183	T8492	T8489	acl	altered,fact
R5184	T8404	T8402	pobj	shRNA,with
R5185	T8405	T8392	punct	", ",differentiated
R5186	T8493	T8494	compound	osteoblast,function
R5187	T8406	T8392	auxpass	were,differentiated
R5188	T8494	T8492	nsubjpass	function,altered
R5189	T8495	T8492	auxpass	is,altered
R519	T1247	T1244	prep	in,resorption
R5190	T8496	T8492	prep	in,altered
R5191	T8513	T8514	compound	bone,mass
R5192	T8497	T8498	nmod	Src,mice
R5193	T8498	T8496	pobj	mice,in
R5194	T8499	T8497	punct	−,Src
R5195	T8514	T8512	pobj	mass,of
R5196	T8500	T8497	punct	/,Src
R5197	T8501	T8497	punct	−,Src
R5198	T8502	T8503	punct	[,61
R5199	T8515	T8511	prep	in,preservation
R52	T289	T290	poss	its,role
R520	T1248	T1249	amod	elderly,men
R5200	T8503	T8492	parataxis	61,altered
R5201	T8504	T8503	nummod	60,61
R5202	T8505	T8503	punct	",",61
R5203	T8516	T8517	det	the,mice
R5204	T8506	T8503	punct	],61
R5205	T8507	T8508	det	the,possibility
R5206	T8508	T8490	dobj	possibility,raises
R5207	T8517	T8515	pobj	mice,in
R5208	T8509	T8510	mark	that,linked
R5209	T8510	T8508	acl	linked,possibility
R521	T1249	T1247	pobj	men,in
R5210	T8511	T8510	nsubjpass	preservation,linked
R5211	T8518	T8517	nmod	Sam68,mice
R5212	T8512	T8511	prep	of,preservation
R5213	T8519	T8518	punct	−,Sam68
R5214	T8520	T8518	punct	/,Sam68
R5215	T8521	T8518	punct	−,Sam68
R5216	T8522	T8510	aux	could,linked
R5217	T8523	T8510	auxpass	be,linked
R5218	T8524	T8510	prep	with,linked
R5219	T8619	T8617	amod	sympathetic,pathway
R522	T1250	T1249	cc	and,men
R5220	T8525	T8510	cc	and,linked
R5221	T8620	T8619	punct	-,sympathetic
R5222	T8621	T8615	acomp	unaltered,is
R5223	T8622	T8615	prep	in,is
R5224	T8526	T8510	conj	regulated,linked
R5225	T8623	T8624	nmod	Sam68,mice
R5226	T8527	T8526	agent	by,regulated
R5227	T8624	T8622	pobj	mice,in
R5228	T8528	T8526	dobj	Src,regulated
R5229	T8625	T8623	punct	−,Sam68
R523	T1251	T1249	conj	women,men
R5230	T8626	T8623	punct	/,Sam68
R5231	T8529	T8490	punct	.,raises
R5232	T8627	T8623	punct	−,Sam68
R5233	T8531	T8532	det	The,pathway
R5234	T8628	T8615	cc	and,is
R5235	T8629	T8630	mark	that,explain
R5236	T8630	T8615	conj	explain,is
R5237	T8631	T8632	det	the,lowering
R5238	T8632	T8630	nsubj	lowering,explain
R5239	T8633	T8632	prep	of,lowering
R524	T1252	T1246	auxpass	is,associated
R5240	T8634	T8633	pobj	leptin,of
R5241	T8532	T8533	nsubjpass	pathway,identified
R5242	T8635	T8630	aux	may,explain
R5243	T8636	T8637	det	the,levels
R5244	T8637	T8630	dobj	levels,explain
R5245	T8534	T8535	prep	by,regulates
R5246	T8638	T8637	amod	lower,levels
R5247	T8639	T8637	prep	of,levels
R5248	T8640	T8639	pobj	CTX,of
R5249	T8535	T8532	relcl	regulates,pathway
R525	T1253	T1246	prep	with,associated
R5250	T8641	T8637	prep	in,levels
R5251	T8642	T8643	det	the,serum
R5252	T8643	T8641	pobj	serum,in
R5253	T8536	T8534	pobj	which,by
R5254	T8644	T8643	prep	of,serum
R5255	T8645	T8646	nmod	Sam68,mice
R5256	T8646	T8644	pobj	mice,of
R5257	T8537	T8535	nsubj	leptin,regulates
R5258	T8647	T8645	punct	−,Sam68
R5259	T8648	T8645	punct	/,Sam68
R526	T1254	T1255	det	a,reduction
R5260	T8649	T8645	punct	−,Sam68
R5261	T8538	T8539	compound	bone,resorption
R5262	T8650	T8612	punct	.,suggest
R5263	T8652	T8653	det	These,findings
R5264	T8539	T8535	dobj	resorption,regulates
R5265	T8653	T8654	nsubj	findings,suggest
R5266	T8654	T8655	ccomp	suggest,results
R5267	T8540	T8533	auxpass	was,identified
R5268	T8656	T8657	mark	that,regulating
R5269	T8657	T8654	ccomp	regulating,suggest
R527	T1255	T1253	pobj	reduction,with
R5270	T8541	T8542	aux	to,involve
R5271	T8658	T8657	nsubj	Sam68,regulating
R5272	T8659	T8657	aux	may,regulating
R5273	T8542	T8533	xcomp	involve,identified
R5274	T8660	T8657	aux	be,regulating
R5275	T8661	T8662	compound	bone,metabolism
R5276	T8662	T8657	dobj	metabolism,regulating
R5277	T8543	T8544	det	the,system
R5278	T8663	T8657	prep	at,regulating
R5279	T8664	T8665	nummod	two,levels
R528	T1256	T1255	prep	in,reduction
R5280	T8665	T8663	pobj	levels,at
R5281	T8544	T8542	dobj	system,involve
R5282	T8666	T8665	amod	different,levels
R5283	T8667	T8655	punct	: ,results
R5284	T8668	T8669	punct	(,1
R5285	T8545	T8544	amod	sympathetic,system
R5286	T8669	T8655	meta	1,results
R5287	T8670	T8669	punct	),1
R5288	T8671	T8672	det	the,absence
R5289	T8672	T8655	nsubj	absence,results
R529	T1257	T1258	compound	bone,mass
R5290	T8673	T8672	prep	of,absence
R5291	T8674	T8673	pobj	Sam68,of
R5292	T8546	T8544	amod	nervous,system
R5293	T8675	T8655	prep	in,results
R5294	T8676	T8677	amod	lower,levels
R5295	T8677	T8675	pobj	levels,in
R5296	T8547	T8544	acl	relaying,system
R5297	T8678	T8677	compound	leptin,levels
R5298	T8679	T8680	dep	that,reduce
R5299	T8680	T8677	relcl	reduce,levels
R53	T290	T267	nsubj	role,remained
R530	T1258	T1256	pobj	mass,in
R5300	T8548	T8547	prep	to,relaying
R5301	T8681	T8680	aux	may,reduce
R5302	T8682	T8683	compound	bone,resorption
R5303	T8683	T8680	dobj	resorption,reduce
R5304	T8549	T8550	det	the,osteoblasts
R5305	T8684	T8680	prep	via,reduce
R5306	T8685	T8686	det	the,system
R5307	T8686	T8684	pobj	system,via
R5308	T8550	T8548	pobj	osteoblasts,to
R5309	T8687	T8686	amod	sympathetic,system
R531	T1259	T1255	cc	and,reduction
R5310	T8688	T8686	amod	nervous,system
R5311	T8689	T8655	cc	and,results
R5312	T8551	T8547	prep	via,relaying
R5313	T8690	T8691	punct	(,2
R5314	T8691	T8692	meta	2,favors
R5315	T8692	T8655	conj	favors,results
R5316	T8552	T8553	det	the,pathway
R5317	T8693	T8691	punct	),2
R5318	T8694	T8695	det	the,absence
R5319	T8695	T8692	nsubj	absence,favors
R532	T1260	T1261	det	an,increase
R5320	T8553	T8551	pobj	pathway,via
R5321	T8696	T8695	prep	of,absence
R5322	T8697	T8696	pobj	Sam68,of
R5323	T8698	T8699	nmod	osteoblast,differentiation
R5324	T8554	T8555	npadvmod	β,adrenergic
R5325	T8699	T8692	dobj	differentiation,favors
R5326	T8700	T8698	punct	", ",osteoblast
R5327	T8701	T8702	advmod	rather,than
R5328	T8555	T8553	amod	adrenergic,pathway
R5329	T8702	T8698	cc	than,osteoblast
R533	T1261	T1255	conj	increase,reduction
R5330	T8703	T8698	conj	adipocyte,osteoblast
R5331	T8556	T8555	punct	-,adrenergic
R5332	T8704	T8699	punct	", ",differentiation
R5333	T8705	T8655	punct	.,results
R5334	T8557	T8547	advcl	leading,relaying
R5335	T8707	T8708	prep	In,define
R5336	T8558	T8557	prep	to,leading
R5337	T8709	T8707	pobj	conclusion,In
R5338	T8710	T8708	punct	", ",define
R5339	T8711	T8712	poss	our,data
R534	T1262	T1261	prep	in,increase
R5340	T8559	T8560	det	the,release
R5341	T8560	T8558	pobj	release,to
R5342	T8712	T8708	nsubj	data,define
R5343	T8713	T8714	det	a,role
R5344	T8714	T8708	dobj	role,define
R5345	T8561	T8560	prep	of,release
R5346	T8715	T8714	amod	physiologic,role
R5347	T8716	T8714	prep	for,role
R5348	T8562	T8563	compound	growth,factors
R5349	T8717	T8716	pobj	Sam68,for
R535	T1263	T1264	amod	circulating,levels
R5350	T8718	T8714	prep	in,role
R5351	T8563	T8561	pobj	factors,of
R5352	T8564	T8563	prep	including,factors
R5353	T8719	T8720	compound	bone,metabolism
R5354	T8565	T8564	pobj	RANKL,including
R5355	T8720	T8718	pobj	metabolism,in
R5356	T8566	T8567	dep	that,causes
R5357	T8721	T8720	cc	and,metabolism
R5358	T8722	T8723	nmod	bone,marrow
R5359	T8567	T8563	relcl	causes,factors
R536	T1264	T1262	pobj	levels,in
R5360	T8723	T8724	nmod	marrow,differentiation
R5361	T8724	T8720	conj	differentiation,metabolism
R5362	T8568	T8569	det	the,osteoclasts
R5363	T8569	T8570	nsubj	osteoclasts,thrive
R5364	T8725	T8726	amod	mesenchymal,cell
R5365	T8570	T8567	ccomp	thrive,causes
R5366	T8726	T8724	compound	cell,differentiation
R5367	T8727	T8726	compound	stem,cell
R5368	T8571	T8570	aux	to,thrive
R5369	T8728	T8708	punct	.,define
R537	T1265	T1264	prep	of,levels
R5370	T8730	T8731	det	The,phenotype
R5371	T8572	T8573	punct	[,8
R5372	T8731	T8733	nsubj	phenotype,imply
R5373	T8732	T8731	compound	bone,phenotype
R5374	T8573	T8533	parataxis	8,identified
R5375	T8734	T8731	acl	observed,phenotype
R5376	T8735	T8734	prep	in,observed
R5377	T8574	T8573	punct	",",8
R5378	T8736	T8737	nmod	Sam68,mice
R5379	T8737	T8735	pobj	mice,in
R538	T1266	T1267	compound	bone,biomarkers
R5380	T8738	T8736	punct	−,Sam68
R5381	T8575	T8573	appos	18,8
R5382	T8739	T8736	punct	/,Sam68
R5383	T8740	T8736	punct	−,Sam68
R5384	T8576	T8577	punct	–,20
R5385	T8741	T8742	mark	that,prevent
R5386	T8742	T8733	ccomp	prevent,imply
R5387	T8743	T8742	nsubj	inhibitors,prevent
R5388	T8577	T8575	prep	20,18
R5389	T8744	T8743	prep	of,inhibitors
R539	T1267	T1265	pobj	biomarkers,of
R5390	T8745	T8744	pobj	Sam68,of
R5391	T8746	T8742	aux	could,prevent
R5392	T8747	T8748	npadvmod	age,related
R5393	T8748	T8750	amod	related,loss
R5394	T8749	T8748	punct	-,related
R5395	T8578	T8573	punct	],8
R5396	T8750	T8742	dobj	loss,prevent
R5397	T8751	T8750	compound	bone,loss
R5398	T8579	T8533	punct	.,identified
R5399	T8752	T8733	punct	.,imply
R54	T291	T290	amod	physiologic,role
R540	T1268	T1269	punct	[,15
R5400	T8754	T8755	advmod	Furthermore,suggest
R5401	T8581	T8582	det	The,lowering
R5402	T8756	T8755	punct	", ",suggest
R5403	T8757	T8758	det	the,results
R5404	T8758	T8755	nsubj	results,suggest
R5405	T8582	T8583	nsubj	lowering,is
R5406	T8759	T8755	advmod	also,suggest
R5407	T8760	T8761	mark	that,influence
R5408	T8761	T8755	ccomp	influence,suggest
R5409	T8584	T8582	prep	of,lowering
R541	T1269	T1246	parataxis	15,associated
R5410	T8762	T8763	compound	Sam68,levels
R5411	T8763	T8761	nsubj	levels,influence
R5412	T8764	T8763	compound	expression,levels
R5413	T8765	T8763	punct	", ",levels
R5414	T8585	T8586	compound	leptin,levels
R5415	T8766	T8763	conj	hypomorphism,levels
R5416	T8767	T8766	punct	", ",hypomorphism
R5417	T8768	T8766	cc	and,hypomorphism
R5418	T8586	T8584	pobj	levels,of
R5419	T8769	T8766	conj	mutations,hypomorphism
R542	T1270	T1269	punct	],15
R5420	T8770	T8763	prep	in,levels
R5421	T8771	T8770	pobj	humans,in
R5422	T8587	T8582	prep	in,lowering
R5423	T8772	T8761	aux	may,influence
R5424	T8773	T8761	dobj	susceptibility,influence
R5425	T8774	T8773	prep	to,susceptibility
R5426	T8588	T8589	amod	aged,mice
R5427	T8775	T8776	compound	marrow,adipocyte
R5428	T8776	T8777	compound	adipocyte,accumulation
R5429	T8777	T8774	pobj	accumulation,to
R543	T1271	T1246	punct	.,associated
R5430	T8589	T8587	pobj	mice,in
R5431	T8778	T8777	cc	and,accumulation
R5432	T8779	T8777	conj	osteoporosis,accumulation
R5433	T8780	T8755	punct	.,suggest
R5434	T8590	T8589	nmod	Sam68,mice
R5435	T8782	T8783	poss	Our,findings
R5436	T8783	T8784	nsubj	findings,identify
R5437	T8591	T8590	punct	−,Sam68
R5438	T8785	T8784	advmod	also,identify
R5439	T8786	T8787	det	a,model
R544	T1273	T1274	det	These,changes
R5440	T8592	T8590	punct	/,Sam68
R5441	T8787	T8784	dobj	model,identify
R5442	T8788	T8787	amod	new,model
R5443	T8789	T8787	compound	animal,model
R5444	T8790	T8791	aux	to,study
R5445	T8791	T8787	advcl	study,model
R5446	T8792	T8793	amod	aging,loss
R5447	T8593	T8590	punct	−,Sam68
R5448	T8793	T8791	dobj	loss,study
R5449	T8794	T8793	compound	bone,loss
R545	T1274	T1275	nsubjpass	changes,attributed
R5450	T8594	T8583	acomp	consistent,is
R5451	T8795	T8784	punct	.,identify
R5452	T8595	T8594	prep	with,consistent
R5453	T8596	T8597	det	these,mice
R5454	T8597	T8598	nsubj	mice,having
R5455	T8598	T8595	pcomp	having,with
R5456	T8599	T8600	det	a,mass
R5457	T8600	T8598	dobj	mass,having
R5458	T8601	T8600	amod	high,mass
R5459	T8602	T8600	compound	bone,mass
R546	T1276	T1275	aux	have,attributed
R5460	T8603	T8598	prep	compared,having
R5461	T8604	T8603	prep	with,compared
R5462	T8605	T8606	poss	their,littermates
R5463	T8606	T8604	pobj	littermates,with
R5464	T8607	T8606	amod	aged,littermates
R5465	T8608	T8583	punct	.,is
R5466	T8610	T8611	det	These,data
R5467	T8611	T8612	nsubj	data,suggest
R5468	T8613	T8612	aux	would,suggest
R5469	T8614	T8615	mark	that,is
R547	T1277	T1275	auxpass	been,attributed
R5470	T8615	T8612	advcl	is,suggest
R5471	T8616	T8617	det	the,pathway
R5472	T8617	T8615	nsubj	pathway,is
R5473	T8618	T8619	npadvmod	leptin,sympathetic
R5474	T8863	T8864	amod	Histologic,analyses
R5475	T8865	T8863	punct	", ",Histologic
R5476	T8866	T8863	conj	immunohistochemical,Histologic
R5477	T8867	T8866	punct	", ",immunohistochemical
R5478	T8868	T8866	cc	and,immunohistochemical
R5479	T8869	T8866	conj	histomorphometric,immunohistochemical
R548	T1278	T1275	advmod	primarily,attributed
R5480	T8870	T8864	punct	.,analyses
R5481	T8872	T8873	det	All,analyses
R5482	T8873	T8874	nsubjpass	analyses,performed
R5483	T8875	T8874	auxpass	were,performed
R5484	T8876	T8877	advmod	essentially,described
R5485	T8877	T8874	advcl	described,performed
R5486	T8878	T8877	mark	as,described
R5487	T8879	T8877	advmod	previously,described
R5488	T8880	T8881	punct	[,63
R5489	T8881	T8874	parataxis	63,performed
R549	T1279	T1275	prep	to,attributed
R5490	T8882	T8881	nummod	62,63
R5491	T8883	T8881	punct	",",63
R5492	T8884	T8881	punct	],63
R5493	T8885	T8874	punct	.,performed
R5494	T8887	T8888	advmod	Briefly,removed
R5495	T8889	T8888	punct	", ",removed
R5496	T8890	T8891	amod	embryonic,mice
R5497	T8891	T8888	nsubjpass	mice,removed
R5498	T8892	T8888	auxpass	were,removed
R5499	T8893	T8888	prep	from,removed
R55	T292	T267	aux	has,remained
R550	T1280	T1281	amod	nutritional,deficits
R5500	T8894	T8895	amod	timed,dams
R5501	T8895	T8893	pobj	dams,from
R5502	T8896	T8895	amod	pregnant,dams
R5503	T8897	T8888	prep	at,removed
R5504	T8898	T8897	pobj	E14.5,at
R5505	T8899	T8898	cc	and,E14.5
R5506	T8900	T8898	conj	E16.5,E14.5
R5507	T8901	T8888	cc	and,removed
R5508	T8902	T8888	conj	fixed,removed
R5509	T8903	T8902	advcl	intact,fixed
R551	T1281	T1279	pobj	deficits,to
R5510	T8904	T8902	prep	for,fixed
R5511	T8905	T8906	nummod	36,h
R5512	T8906	T8904	pobj	h,for
R5513	T8907	T8902	prep	in,fixed
R5514	T8908	T8909	nummod	4,%
R5515	T8909	T8910	compound	%,paraformaldehyde
R5516	T8910	T8907	pobj	paraformaldehyde,in
R5517	T8911	T8902	punct	", ",fixed
R5518	T8912	T8902	conj	rinsed,fixed
R5519	T8913	T8912	advmod	thoroughly,rinsed
R552	T1282	T1281	acl	resulting,deficits
R5520	T8914	T8912	prep	in,rinsed
R5521	T8915	T8914	pobj	PBS,in
R5522	T8916	T8912	punct	", ",rinsed
R5523	T8917	T8912	cc	and,rinsed
R5524	T8918	T8912	conj	processed,rinsed
R5525	T8919	T8918	prep	for,processed
R5526	T8920	T8921	compound	paraffin,embedding
R5527	T8921	T8919	pobj	embedding,for
R5528	T8922	T8888	punct	.,removed
R5529	T8924	T8925	amod	Serial,sections
R553	T1283	T1282	prep	in,resulting
R5530	T8925	T8929	nsubjpass	sections,cut
R5531	T8926	T8927	nummod	4,μm
R5532	T8927	T8925	compound	μm,sections
R5533	T8928	T8927	punct	-,μm
R5534	T8930	T8929	auxpass	were,cut
R5535	T8931	T8929	prep	on,cut
R5536	T8932	T8933	det	a,microtome
R5537	T8933	T8931	pobj	microtome,on
R5538	T8934	T8933	amod	modified,microtome
R5539	T8935	T8936	nmod	Leica,RM
R554	T1284	T1283	pobj	alterations,in
R5540	T8936	T8933	nmod	RM,microtome
R5541	T8937	T8936	nummod	2155,RM
R5542	T8938	T8933	amod	rotary,microtome
R5543	T8939	T8940	punct	(,Microsystems
R5544	T8940	T8933	parataxis	Microsystems,microtome
R5545	T8941	T8940	compound	Leica,Microsystems
R5546	T8942	T8940	punct	", ",Microsystems
R5547	T8943	T8944	compound	Richmond,Hill
R5548	T8944	T8940	npadvmod	Hill,Microsystems
R5549	T8945	T8940	punct	", ",Microsystems
R555	T1285	T1284	prep	in,alterations
R5550	T8946	T8940	npadvmod	Ontario,Microsystems
R5551	T8947	T8940	punct	", ",Microsystems
R5552	T8948	T8940	npadvmod	Canada,Microsystems
R5553	T8949	T8940	punct	),Microsystems
R5554	T8950	T8929	punct	", ",cut
R5555	T8951	T8929	conj	stained,cut
R5556	T8952	T8951	prep	with,stained
R5557	T8953	T8954	det	a,dilution
R5558	T8954	T8952	pobj	dilution,with
R5559	T8955	T8954	nummod	1,dilution
R556	T1286	T1287	det	the,axis
R5560	T8956	T8957	punct	:,600
R5561	T8957	T8955	prep	600,1
R5562	T8958	T8954	prep	of,dilution
R5563	T8959	T8960	det	the,antibody
R5564	T8960	T8958	pobj	antibody,of
R5565	T8961	T8960	nmod	AD1,antibody
R5566	T8962	T8960	amod	anti-Sam68,antibody
R5567	T8963	T8964	punct	[,46
R5568	T8964	T8951	parataxis	46,stained
R5569	T8965	T8964	punct	],46
R557	T1287	T1285	pobj	axis,in
R5570	T8966	T8951	cc	and,stained
R5571	T8967	T8951	conj	counterstained,stained
R5572	T8968	T8967	prep	with,counterstained
R5573	T8969	T8970	preconj	either,green
R5574	T8970	T8968	pobj	green,with
R5575	T8971	T8970	compound	methyl,green
R5576	T8972	T8970	cc	or,green
R5577	T8973	T8970	conj	hematoxylin,green
R5578	T8974	T8929	punct	.,cut
R5579	T8976	T8977	amod	Adult,mice
R558	T1288	T1289	nmod	parathyroid,hormone
R5580	T8977	T8978	nsubj	mice,given
R5581	T8979	T8978	aux	were,given
R5582	T8980	T8981	det	an,injection
R5583	T8981	T8978	dobj	injection,given
R5584	T8982	T8981	amod	intraperitoneal,injection
R5585	T8983	T8981	prep	of,injection
R5586	T8984	T8985	nummod	30,mg
R5587	T8985	T8986	nmod	mg,calcein
R5588	T8986	T8983	pobj	calcein,of
R5589	T8987	T8988	punct	/,kg
R559	T1289	T1287	nmod	hormone,axis
R5590	T8988	T8985	prep	kg,mg
R5591	T8989	T8978	prep	at,given
R5592	T8990	T8991	nummod	7,days
R5593	T8991	T8989	pobj	days,at
R5594	T8992	T8978	cc	and,given
R5595	T8993	T8994	nummod	30,mg
R5596	T8994	T8995	nmod	mg,tetracycline
R5597	T8995	T8978	conj	tetracycline,given
R5598	T8996	T8997	punct	/,kg
R5599	T8997	T8994	prep	kg,mg
R56	T293	T267	acomp	elusive,remained
R560	T1290	T1289	punct	–,hormone
R5600	T8998	T8995	prep	at,tetracycline
R5601	T8999	T9000	nummod	2,days
R5602	T9000	T8998	pobj	days,at
R5603	T9001	T9002	amod	prior,to
R5604	T9002	T9000	prep	to,days
R5605	T9003	T9002	pobj	sacrifice,to
R5606	T9004	T9005	aux	to,label
R5607	T9005	T8978	advcl	label,given
R5608	T9006	T9007	advmod	actively,mineralizing
R5609	T9007	T9008	amod	mineralizing,surfaces
R561	T1291	T1292	compound	vitamin,D
R5610	T9008	T9005	dobj	surfaces,label
R5611	T9009	T8978	punct	.,given
R5612	T9011	T9012	prep	After,embedded
R5613	T9013	T9014	advmod	overnight,fixation
R5614	T9014	T9011	pobj	fixation,After
R5615	T9015	T9014	prep	in,fixation
R5616	T9016	T9017	nummod	4,%
R5617	T9017	T9018	compound	%,paraformaldehyde
R5618	T9018	T9015	pobj	paraformaldehyde,in
R5619	T9019	T9014	cc	and,fixation
R562	T1292	T1289	appos	D,hormone
R5620	T9020	T9014	conj	rinsing,fixation
R5621	T9021	T9020	prep	in,rinsing
R5622	T9022	T9021	pobj	PBS,in
R5623	T9023	T9012	punct	", ",embedded
R5624	T9024	T9025	det	the,femur
R5625	T9025	T9012	nsubjpass	femur,embedded
R5626	T9026	T9025	amod	left,femur
R5627	T9027	T9025	cc	and,femur
R5628	T9028	T9025	conj	tibia,femur
R5629	T9029	T9012	auxpass	were,embedded
R563	T1293	T1294	punct	[,16
R5630	T9030	T9012	prep	in,embedded
R5631	T9031	T9030	pobj	polymethylmethacrylate,in
R5632	T9032	T9031	punct	(,polymethylmethacrylate
R5633	T9033	T9031	appos	MMA,polymethylmethacrylate
R5634	T9034	T9031	punct	),polymethylmethacrylate
R5635	T9035	T9031	cc	or,polymethylmethacrylate
R5636	T9036	T9037	det	a,mixture
R5637	T9037	T9031	conj	mixture,polymethylmethacrylate
R5638	T9038	T9037	prep	of,mixture
R5639	T9039	T9040	nummod	50,%
R564	T1294	T1281	parataxis	16,deficits
R5640	T9040	T9041	compound	%,MMA
R5641	T9041	T9038	pobj	MMA,of
R5642	T9042	T9041	cc	and,MMA
R5643	T9043	T9044	nummod	50,%
R5644	T9044	T9041	conj	%,MMA
R5645	T9045	T9044	appos	glycolmethacrylate,%
R5646	T9046	T9045	punct	(,glycolmethacrylate
R5647	T9047	T9045	appos	GMA,glycolmethacrylate
R5648	T9048	T9037	punct	),mixture
R5649	T9049	T9012	punct	.,embedded
R565	T1295	T1294	punct	],16
R5650	T9051	T9052	amod	Serial,sections
R5651	T9052	T9059	nsubjpass	sections,left
R5652	T9053	T9054	quantmod	4,6
R5653	T9054	T9057	nummod	6,μm
R5654	T9055	T9054	punct	-,6
R5655	T9056	T9054	quantmod	to,6
R5656	T9057	T9052	compound	μm,sections
R5657	T9058	T9057	punct	-,μm
R5658	T9060	T9052	prep	of,sections
R5659	T9061	T9062	npadvmod	MMA,embedded
R566	T1296	T1279	punct	", ",to
R5660	T9062	T9064	amod	embedded,tissues
R5661	T9063	T9062	punct	-,embedded
R5662	T9064	T9060	pobj	tissues,of
R5663	T9065	T9059	auxpass	were,left
R5664	T9066	T9059	oprd	unstained,left
R5665	T9067	T9059	cc	or,left
R5666	T9068	T9059	conj	stained,left
R5667	T9069	T9068	prep	with,stained
R5668	T9070	T9071	compound	von,Kossa
R5669	T9071	T9069	pobj	Kossa,with
R567	T1297	T1279	conj	to,to
R5670	T9072	T9071	cc	and,Kossa
R5671	T9073	T9074	compound	toluidine,blue
R5672	T9074	T9071	conj	blue,Kossa
R5673	T9075	T9069	cc	or,with
R5674	T9076	T9069	conj	with,with
R5675	T9077	T9078	compound	toluidine,blue
R5676	T9078	T9076	pobj	blue,with
R5677	T9079	T9078	advmod	alone,blue
R5678	T9080	T9059	punct	", ",left
R5679	T9081	T9082	mark	while,stained
R568	T1298	T1299	amod	gonadal,deficiency
R5680	T9082	T9059	advcl	stained,left
R5681	T9083	T9084	nummod	4,μm
R5682	T9084	T9086	compound	μm,sections
R5683	T9085	T9084	punct	-,μm
R5684	T9086	T9082	nsubjpass	sections,stained
R5685	T9087	T9088	compound	MMA,GMA
R5686	T9088	T9086	compound	GMA,sections
R5687	T9089	T9088	punct	-,GMA
R5688	T9090	T9082	auxpass	were,stained
R5689	T9091	T9082	prep	for,stained
R569	T1299	T1297	pobj	deficiency,to
R5690	T9092	T9093	nmod	TRAP,activity
R5691	T9093	T9091	pobj	activity,for
R5692	T9094	T9092	cc	and,TRAP
R5693	T9095	T9092	conj	ALP,TRAP
R5694	T9096	T9059	punct	.,left
R5695	T9098	T9099	nsubjpass	Images,captured
R5696	T9100	T9099	auxpass	were,captured
R5697	T9101	T9099	advcl	using,captured
R5698	T9102	T9103	det	a,microscope
R5699	T9103	T9101	dobj	microscope,using
R57	T294	T267	punct	.,remained
R570	T1300	T1299	compound	hormone,deficiency
R5700	T9104	T9105	compound	Leica,DMR
R5701	T9105	T9103	compound	DMR,microscope
R5702	T9106	T9107	punct	(,Microsystems
R5703	T9107	T9103	parataxis	Microsystems,microscope
R5704	T9108	T9107	compound	Leica,Microsystems
R5705	T9109	T9107	punct	),Microsystems
R5706	T9110	T9103	acl	equipped,microscope
R5707	T9111	T9110	prep	with,equipped
R5708	T9112	T9113	det	a,camera
R5709	T9113	T9111	pobj	camera,with
R571	T1301	T1302	punct	[,17
R5710	T9114	T9113	nmod	Retiga,camera
R5711	T9115	T9114	nummod	1300,Retiga
R5712	T9116	T9117	punct	(,Qimaging
R5713	T9117	T9113	parataxis	Qimaging,camera
R5714	T9118	T9117	punct	", ",Qimaging
R5715	T9119	T9117	npadvmod	Burnaby,Qimaging
R5716	T9120	T9117	punct	", ",Qimaging
R5717	T9121	T9122	compound	British,Columbia
R5718	T9122	T9117	npadvmod	Columbia,Qimaging
R5719	T9123	T9117	punct	", ",Qimaging
R572	T1302	T1299	parataxis	17,deficiency
R5720	T9124	T9117	npadvmod	Canada,Qimaging
R5721	T9125	T9117	punct	),Qimaging
R5722	T9126	T9099	cc	and,captured
R5723	T9127	T9128	det	the,data
R5724	T9128	T9131	nsubj	data,obtained
R5725	T9129	T9128	amod	primary,data
R5726	T9130	T9128	amod	histomorphometric,data
R5727	T9131	T9099	conj	obtained,captured
R5728	T9132	T9131	advcl	using,obtained
R5729	T9133	T9134	compound	Bioquant,Prime
R573	T1303	T1302	punct	],17
R5730	T9134	T9136	compound	Prime,software
R5731	T9135	T9134	compound	Nova,Prime
R5732	T9136	T9132	dobj	software,using
R5733	T9137	T9138	compound	image,analysis
R5734	T9138	T9136	compound	analysis,software
R5735	T9139	T9140	punct	(,Corp
R5736	T9140	T9136	parataxis	Corp,software
R5737	T9141	T9140	compound	Bioquant,Corp
R5738	T9142	T9143	compound	Image,Analysis
R5739	T9143	T9140	compound	Analysis,Corp
R574	T1304	T1297	punct	", ",to
R5740	T9144	T9140	punct	", ",Corp
R5741	T9145	T9140	npadvmod	Nashville,Corp
R5742	T9146	T9140	punct	", ",Corp
R5743	T9147	T9140	npadvmod	Tennessee,Corp
R5744	T9148	T9140	punct	", ",Corp
R5745	T9149	T9150	compound	United,States
R5746	T9150	T9140	npadvmod	States,Corp
R5747	T9151	T9140	punct	),Corp
R5748	T9152	T9131	punct	.,obtained
R5749	T9154	T9155	nsubj	Nomenclature,conform
R575	T1305	T1297	conj	to,to
R5750	T9156	T9154	cc	and,Nomenclature
R5751	T9157	T9154	conj	abbreviations,Nomenclature
R5752	T9158	T9155	prep	to,conform
R5753	T9159	T9158	pobj	those,to
R5754	T9160	T9159	acl	recommended,those
R5755	T9161	T9160	agent	by,recommended
R5756	T9162	T9163	det	the,Society
R5757	T9163	T9161	pobj	Society,by
R5758	T9164	T9163	compound	American,Society
R5759	T9165	T9163	prep	for,Society
R576	T1306	T1307	compound	leptin,levels
R5760	T9166	T9167	nmod	Bone,Research
R5761	T9167	T9165	pobj	Research,for
R5762	T9168	T9166	cc	and,Bone
R5763	T9169	T9166	conj	Mineral,Bone
R5764	T9170	T9171	punct	[,50
R5765	T9171	T9155	parataxis	50,conform
R5766	T9172	T9171	punct	],50
R5767	T9173	T9155	punct	.,conform
R577	T1307	T1305	pobj	levels,to
R5772	T9294	T9293	prep	of,Generation
R5773	T9295	T9294	pobj	mice,of
R5774	T9296	T9295	prep	with,mice
R5775	T9297	T9298	amod	targeted,disruption
R5776	T9298	T9296	pobj	disruption,with
R5777	T9299	T9298	prep	of,disruption
R5778	T9300	T9301	det	the,gene
R5779	T9301	T9299	pobj	gene,of
R578	T1308	T1307	cc	and,levels
R5780	T9302	T9301	compound	sam68,gene
R5781	T9303	T9293	punct	.,Generation
R5782	T9305	T9306	det	A,clone
R5783	T9306	T9309	nsubjpass	clone,isolated
R5784	T9307	T9306	compound	λ,clone
R5785	T9308	T9306	compound	bacteriophage,clone
R5786	T9310	T9306	acl	encompassing,clone
R5787	T9311	T9312	nmod	Sam68,3
R5788	T9312	T9310	dobj	3,encompassing
R5789	T9313	T9312	nmod	exons,3
R579	T1309	T1310	det	the,system
R5790	T9314	T9312	prep	to,3
R5791	T9315	T9314	pobj	9,to
R5792	T9316	T9309	auxpass	was,isolated
R5793	T9317	T9309	prep	from,isolated
R5794	T9318	T9319	det	a,library
R5795	T9319	T9317	pobj	library,from
R5796	T9320	T9321	nummod	129,SvJ
R5797	T9321	T9319	nmod	SvJ,library
R5798	T9322	T9321	punct	/,SvJ
R5799	T9323	T9319	amod	genomic,library
R58	T296	T297	advmod	Herein,report
R580	T1310	T1307	conj	system,levels
R5800	T9324	T9309	advcl	using,isolated
R5801	T9325	T9326	amod	full,length
R5802	T9326	T9328	compound	length,cDNA
R5803	T9327	T9326	punct	-,length
R5804	T9328	T9324	dobj	cDNA,using
R5805	T9329	T9328	compound	Sam68,cDNA
R5806	T9330	T9324	prep	as,using
R5807	T9331	T9332	det	a,probe
R5808	T9332	T9330	pobj	probe,as
R5809	T9333	T9309	punct	.,isolated
R581	T1311	T1310	amod	sympathetic,system
R5810	T9335	T9336	npadvmod	XbaI,digested
R5811	T9336	T9338	amod	digested,fragments
R5812	T9337	T9336	punct	-,digested
R5813	T9338	T9342	nsubjpass	fragments,subcloned
R5814	T9339	T9338	nmod	Sam68,fragments
R5815	T9340	T9338	amod	genomic,fragments
R5816	T9341	T9338	compound	DNA,fragments
R5817	T9343	T9338	prep	of,fragments
R5818	T9344	T9345	nummod	4,kb
R5819	T9345	T9343	pobj	kb,of
R582	T1312	T1310	amod	nervous,system
R5820	T9346	T9347	punct	(,encompassing
R5821	T9347	T9345	parataxis	encompassing,kb
R5822	T9348	T9347	dobj	exon,encompassing
R5823	T9349	T9348	nummod	4,exon
R5824	T9350	T9348	cc	and,exon
R5825	T9351	T9348	conj	part,exon
R5826	T9352	T9351	prep	of,part
R5827	T9353	T9352	pobj	exon,of
R5828	T9354	T9353	nummod	5,exon
R5829	T9355	T9347	punct	),encompassing
R583	T1313	T1314	punct	[,8
R5830	T9356	T9345	cc	and,kb
R5831	T9357	T9345	conj	3kb,kb
R5832	T9358	T9359	punct	(,spanning
R5833	T9359	T9357	parataxis	spanning,3kb
R5834	T9360	T9359	dobj	part,spanning
R5835	T9361	T9360	prep	of,part
R5836	T9362	T9361	pobj	exon,of
R5837	T9363	T9362	nummod	5,exon
R5838	T9364	T9360	cc	and,part
R5839	T9365	T9360	conj	exon,part
R584	T1314	T1310	parataxis	8,system
R5840	T9366	T9365	nummod	6,exon
R5841	T9367	T9359	punct	),spanning
R5842	T9368	T9342	auxpass	were,subcloned
R5843	T9369	T9342	prep	in,subcloned
R5844	T9370	T9371	compound	Bluescript,SK
R5845	T9371	T9369	pobj	SK,in
R5846	T9372	T9342	advcl	resulting,subcloned
R5847	T9373	T9372	prep	in,resulting
R5848	T9374	T9373	pobj	pBS4,in
R5849	T9375	T9374	cc	and,pBS4
R585	T1315	T1314	punct	",",8
R5850	T9376	T9374	conj	pBS3,pBS4
R5851	T9377	T9372	punct	", ",resulting
R5852	T9378	T9372	advmod	respectively,resulting
R5853	T9379	T9342	punct	.,subcloned
R5854	T9381	T9382	det	A,fragment
R5855	T9382	T9384	nsubjpass	fragment,amplified
R5856	T9383	T9382	compound	DNA,fragment
R5857	T9385	T9384	auxpass	was,amplified
R5858	T9386	T9384	prep	from,amplified
R5859	T9387	T9386	pobj	pBS4,from
R586	T1316	T1314	appos	18,8
R5860	T9388	T9384	prep	with,amplified
R5861	T9389	T9390	det	the,oligonucleotides
R5862	T9390	T9388	pobj	oligonucleotides,with
R5863	T9391	T9390	amod	following,oligonucleotides
R5864	T9392	T9393	punct	(,5
R5865	T9393	T9390	parataxis	5,oligonucleotides
R5866	T9394	T9393	punct	′,5
R5867	T9395	T9393	punct	-,5
R5868	T9396	T9397	compound	AAT,C
R5869	T9397	T9393	appos	C,5
R587	T1317	T1318	punct	–,20
R5870	T9398	T9397	compound	GTC,C
R5871	T9399	T9397	compound	TAG,C
R5872	T9400	T9397	compound	AAA,C
R5873	T9401	T9397	compound	CAA,C
R5874	T9402	T9397	compound	CTC,C
R5875	T9403	T9397	compound	ATA,C
R5876	T9404	T9397	compound	TAC,C
R5877	T9405	T9397	compound	AGA,C
R5878	T9406	T9393	punct	-,5
R5879	T9407	T9393	nummod	3,5
R588	T1318	T1316	prep	20,18
R5880	T9408	T9393	punct	′,5
R5881	T9409	T9393	punct	),5
R5882	T9410	T9390	cc	and,oligonucleotides
R5883	T9411	T9412	det	the,primer
R5884	T9412	T9390	conj	primer,oligonucleotides
R5885	T9413	T9412	amod	universal,primer
R5886	T9414	T9384	punct	.,amplified
R5887	T9416	T9417	det	The,fragment
R5888	T9417	T9425	nsubjpass	fragment,subcloned
R5889	T9418	T9419	npadvmod	XbaI,digested
R589	T1319	T1314	punct	],8
R5890	T9419	T9417	amod	digested,fragment
R5891	T9420	T9419	punct	-,digested
R5892	T9421	T9422	nummod	1,kb
R5893	T9422	T9417	compound	kb,fragment
R5894	T9423	T9422	punct	-,kb
R5895	T9424	T9417	compound	DNA,fragment
R5896	T9426	T9425	auxpass	was,subcloned
R5897	T9427	T9425	prep	in,subcloned
R5898	T9428	T9429	det	the,site
R5899	T9429	T9427	pobj	site,in
R59	T298	T297	nsubj	we,report
R590	T1320	T1305	punct	", ",to
R5900	T9430	T9429	compound	XbaI,site
R5901	T9431	T9429	prep	of,site
R5902	T9432	T9431	pobj	pPNT,of
R5903	T9433	T9434	punct	(,gift
R5904	T9434	T9432	parataxis	gift,pPNT
R5905	T9435	T9434	det	a,gift
R5906	T9436	T9434	prep	from,gift
R5907	T9437	T9438	compound	Andrew,Karaplis
R5908	T9438	T9436	pobj	Karaplis,from
R5909	T9439	T9438	punct	", ",Karaplis
R591	T1321	T1305	cc	and,to
R5910	T9440	T9441	compound	McGill,University
R5911	T9441	T9438	npadvmod	University,Karaplis
R5912	T9442	T9438	punct	", ",Karaplis
R5913	T9443	T9438	npadvmod	Montréal,Karaplis
R5914	T9444	T9438	punct	", ",Karaplis
R5915	T9445	T9438	npadvmod	Quebec,Karaplis
R5916	T9446	T9438	punct	", ",Karaplis
R5917	T9447	T9438	npadvmod	Canada,Karaplis
R5918	T9448	T9434	punct	),gift
R5919	T9449	T9425	punct	.,subcloned
R592	T1322	T1305	conj	to,to
R5920	T9451	T9452	det	The,fragment
R5921	T9452	T9456	nsubjpass	fragment,amplified
R5922	T9453	T9454	nummod	3,kb
R5923	T9454	T9452	compound	kb,fragment
R5924	T9455	T9454	punct	-,kb
R5925	T9457	T9452	prep	from,fragment
R5926	T9458	T9457	pobj	pBS3,from
R5927	T9459	T9456	auxpass	was,amplified
R5928	T9460	T9456	prep	by,amplified
R5929	T9461	T9460	pobj	PCR,by
R593	T1323	T1322	pobj	alterations,to
R5930	T9462	T9461	prep	with,PCR
R5931	T9463	T9462	punct	(,with
R5932	T9464	T9462	pobj	5,with
R5933	T9465	T9464	punct	′,5
R5934	T9466	T9464	punct	-,5
R5935	T9467	T9468	compound	GGG,G
R5936	T9468	T9464	appos	G,5
R5937	T9469	T9468	compound	ATG,G
R5938	T9470	T9468	compound	CGG,G
R5939	T9471	T9468	compound	CCG,G
R594	T1324	T1323	prep	in,alterations
R5940	T9472	T9468	compound	CTC,G
R5941	T9473	T9468	compound	TAG,G
R5942	T9474	T9468	compound	AAT,G
R5943	T9475	T9468	compound	TGT,G
R5944	T9476	T9468	compound	CCT,G
R5945	T9477	T9468	compound	ACT,G
R5946	T9478	T9468	compound	TGA,G
R5947	T9479	T9468	compound	ACG,G
R5948	T9480	T9464	punct	-,5
R5949	T9481	T9464	nummod	3,5
R595	T1325	T1326	compound	bone,cell
R5950	T9482	T9464	punct	',5
R5951	T9483	T9464	punct	),5
R5952	T9484	T9464	cc	and,5
R5953	T9485	T9486	punct	(,5
R5954	T9486	T9464	conj	5,5
R5955	T9487	T9486	punct	′,5
R5956	T9488	T9486	punct	-,5
R5957	T9489	T9490	compound	CGG,A
R5958	T9490	T9486	appos	A,5
R5959	T9491	T9490	compound	TGG,A
R596	T1326	T1327	compound	cell,apoptosis
R5960	T9492	T9490	compound	CGG,A
R5961	T9493	T9490	compound	CCG,A
R5962	T9494	T9490	compound	CTG,A
R5963	T9495	T9490	compound	TCG,A
R5964	T9496	T9490	compound	ACC,A
R5965	T9497	T9490	compound	TGA,A
R5966	T9498	T9490	compound	GTA,A
R5967	T9499	T9490	compound	ACA,A
R5968	T9500	T9490	compound	TTT,A
R5969	T9501	T9490	compound	CTT,A
R597	T1327	T1324	pobj	apoptosis,in
R5970	T9502	T9486	punct	-,5
R5971	T9503	T9486	nummod	3,5
R5972	T9504	T9486	punct	′,5
R5973	T9505	T9456	punct	),amplified
R5974	T9506	T9456	cc	and,amplified
R5975	T9507	T9456	conj	subcloned,amplified
R5976	T9508	T9507	prep	in,subcloned
R5977	T9509	T9510	det	the,site
R5978	T9510	T9508	pobj	site,in
R5979	T9511	T9510	compound	NotI,site
R598	T1328	T1329	punct	[,21
R5980	T9512	T9510	prep	of,site
R5981	T9513	T9512	pobj	pPNT,of
R5982	T9514	T9456	punct	.,amplified
R5983	T9516	T9517	det	The,vector
R5984	T9517	T9519	nsubj	vector,replaces
R5985	T9518	T9517	compound	targeting,vector
R5986	T9520	T9521	compound	pPNT,Sam68
R5987	T9521	T9517	appos	Sam68,vector
R5988	T9522	T9521	punct	-,Sam68
R5989	T9523	T9519	dobj	exon,replaces
R599	T1329	T1323	parataxis	21,alterations
R5990	T9524	T9523	nummod	4,exon
R5991	T9525	T9523	cc	and,exon
R5992	T9526	T9523	conj	part,exon
R5993	T9527	T9526	prep	of,part
R5994	T9528	T9527	pobj	exon,of
R5995	T9529	T9528	nummod	5,exon
R5996	T9530	T9519	prep	with,replaces
R5997	T9531	T9532	det	a,cassette
R5998	T9532	T9530	pobj	cassette,with
R5999	T9533	T9534	npadvmod	neomycin,resistant
R6	T240	T241	det	the,Protein
R60	T299	T300	det	the,generation
R600	T1330	T1329	punct	],21
R6000	T9534	T9532	amod	resistant,cassette
R6001	T9535	T9534	punct	-,resistant
R6002	T9536	T9532	compound	gene,cassette
R6003	T9537	T9519	punct	.,replaces
R6004	T9539	T9540	det	An,site
R6005	T9540	T9542	nsubjpass	site,introduced
R6006	T9541	T9540	compound	SalI,site
R6007	T9543	T9542	auxpass	was,introduced
R6008	T9544	T9542	prep	at,introduced
R6009	T9545	T9546	det	the,end
R601	T1331	T1275	punct	.,attributed
R6010	T9546	T9544	pobj	end,at
R6011	T9547	T9546	nummod	3,end
R6012	T9548	T9547	punct	′,3
R6013	T9549	T9546	prep	of,end
R6014	T9550	T9551	det	the,fragment
R6015	T9551	T9549	pobj	fragment,of
R6016	T9552	T9553	nummod	3,kb
R6017	T9553	T9551	compound	kb,fragment
R6018	T9554	T9553	punct	-,kb
R6019	T9555	T9551	compound	DNA,fragment
R602	T1333	T1334	compound	Bone,loss
R6020	T9556	T9542	cc	and,introduced
R6021	T9557	T9558	auxpass	was,used
R6022	T9558	T9542	conj	used,introduced
R6023	T9559	T9560	aux	to,linearize
R6024	T9560	T9558	advcl	linearize,used
R6025	T9561	T9562	det	the,plasmid
R6026	T9562	T9560	dobj	plasmid,linearize
R6027	T9563	T9560	prep	for,linearize
R6028	T9564	T9563	pobj	electroporation,for
R6029	T9565	T9564	prep	into,electroporation
R603	T1334	T1335	nsubjpass	loss,attributed
R6030	T9566	T9567	amod	embryonic,stem
R6031	T9567	T9568	nmod	stem,cells
R6032	T9568	T9565	pobj	cells,into
R6033	T9569	T9567	punct	(,stem
R6034	T9570	T9567	appos	ES,stem
R6035	T9571	T9568	punct	),cells
R6036	T9572	T9542	punct	.,introduced
R6037	T9574	T9575	advmod	Approximately,"1,000"
R6038	T9575	T9576	nummod	"1,000",colonies
R6039	T9576	T9578	nsubjpass	colonies,screened
R604	T1336	T1334	prep	in,loss
R6040	T9577	T9576	compound	ES,colonies
R6041	T9579	T9578	auxpass	were,screened
R6042	T9580	T9578	cc	and,screened
R6043	T9581	T9582	nummod	two,clones
R6044	T9582	T9583	nsubjpass	clones,identified
R6045	T9583	T9578	conj	identified,screened
R6046	T9584	T9583	auxpass	were,identified
R6047	T9585	T9586	dep	that,contained
R6048	T9586	T9583	ccomp	contained,identified
R6049	T9587	T9588	det	the,Sam68
R605	T1337	T1338	det	the,elderly
R6050	T9588	T9589	compound	Sam68,allele
R6051	T9589	T9586	dobj	allele,contained
R6052	T9590	T9589	compound	mutant,allele
R6053	T9591	T9583	punct	", ",identified
R6054	T9592	T9593	mark	as,determined
R6055	T9593	T9583	advcl	determined,identified
R6056	T9594	T9593	prep	by,determined
R6057	T9595	T9596	compound	Southern,blotting
R6058	T9596	T9594	pobj	blotting,by
R6059	T9597	T9583	punct	.,identified
R606	T1338	T1336	pobj	elderly,in
R6060	T9599	T9600	amod	Targeted,cells
R6061	T9600	T9602	nsubjpass	cells,injected
R6062	T9601	T9600	compound	ES,cells
R6063	T9603	T9602	auxpass	were,injected
R6064	T9604	T9602	prep	into,injected
R6065	T9605	T9606	nummod	3.5,day
R6066	T9606	T9608	npadvmod	day,old
R6067	T9607	T9606	punct	-,day
R6068	T9608	T9610	amod	old,blastocysts
R6069	T9609	T9608	punct	-,old
R607	T1339	T1335	aux	has,attributed
R6070	T9610	T9604	pobj	blastocysts,into
R6071	T9611	T9612	compound	BALB,c
R6072	T9612	T9610	compound	c,blastocysts
R6073	T9613	T9612	punct	/,c
R6074	T9614	T9602	cc	and,injected
R6075	T9615	T9616	auxpass	were,transferred
R6076	T9616	T9602	conj	transferred,injected
R6077	T9617	T9616	prep	into,transferred
R6078	T9618	T9619	nmod	CD,mothers
R6079	T9619	T9617	pobj	mothers,into
R608	T1340	T1335	advmod	also,attributed
R6080	T9620	T9618	punct	-,CD
R6081	T9621	T9618	nummod	1,CD
R6082	T9622	T9619	compound	foster,mothers
R6083	T9623	T9602	punct	", ",injected
R6084	T9624	T9602	cc	and,injected
R6085	T9625	T9626	nsubjpass	animals,mated
R6086	T9626	T9602	conj	mated,injected
R6087	T9627	T9625	acl	classified,animals
R6088	T9628	T9627	prep	as,classified
R6089	T9629	T9628	pobj	chimeras,as
R609	T1341	T1335	auxpass	been,attributed
R6090	T9630	T9627	prep	by,classified
R6091	T9631	T9632	compound	coat,color
R6092	T9632	T9630	pobj	color,by
R6093	T9633	T9626	auxpass	were,mated
R6094	T9634	T9626	prep	with,mated
R6095	T9635	T9636	compound	BALB,c
R6096	T9636	T9638	compound	c,mice
R6097	T9637	T9636	punct	/,c
R6098	T9638	T9634	pobj	mice,with
R6099	T9639	T9626	punct	.,mated
R61	T300	T297	dobj	generation,report
R610	T1342	T1335	prep	to,attributed
R6100	T9641	T9642	compound	Germ,line
R6101	T9642	T9643	compound	line,transmission
R6102	T9643	T9644	nsubjpass	transmission,achieved
R6103	T9645	T9644	auxpass	was,achieved
R6104	T9646	T9644	cc	and,achieved
R6105	T9647	T9648	det	the,mice
R6106	T9648	T9649	nsubjpass	mice,maintained
R6107	T9649	T9644	conj	maintained,achieved
R6108	T9650	T9649	auxpass	were,maintained
R6109	T9651	T9649	prep	in,maintained
R611	T1343	T1342	pobj	alterations,to
R6110	T9652	T9653	nmod	C57BL,background
R6111	T9653	T9651	pobj	background,in
R6112	T9654	T9652	punct	/,C57BL
R6113	T9655	T9652	nummod	6,C57BL
R6114	T9656	T9649	punct	.,maintained
R6115	T9658	T9659	det	The,mice
R6116	T9659	T9660	nsubj	mice,represent
R6117	T9660	T9665	ccomp	represent,maintained
R6118	T9661	T9659	acl	used,mice
R6119	T9662	T9661	prep	for,used
R612	T1344	T1343	prep	in,alterations
R6120	T9663	T9664	det	this,study
R6121	T9664	T9662	pobj	study,for
R6122	T9666	T9660	dobj	mice,represent
R6123	T9667	T9668	dep	that,backcrossed
R6124	T9668	T9666	relcl	backcrossed,mice
R6125	T9669	T9668	auxpass	were,backcrossed
R6126	T9670	T9668	prep	in,backcrossed
R6127	T9671	T9670	pobj	C57BL,in
R6128	T9672	T9671	punct	/,C57BL
R6129	T9673	T9671	nummod	6,C57BL
R613	T1345	T1346	det	the,response
R6130	T9674	T9675	quantmod	between,three
R6131	T9675	T9676	nummod	three,generations
R6132	T9676	T9668	npadvmod	generations,backcrossed
R6133	T9677	T9675	cc	and,three
R6134	T9678	T9675	conj	eight,three
R6135	T9679	T9665	punct	;,maintained
R6136	T9680	T9665	prep	in,maintained
R6137	T9681	T9680	pobj	addition,in
R6138	T9682	T9665	punct	", ",maintained
R6139	T9683	T9665	nsubj	we,maintained
R614	T1346	T1344	pobj	response,in
R6140	T9684	T9685	det	the,mice
R6141	T9685	T9665	dobj	mice,maintained
R6142	T9686	T9685	prep	in,mice
R6143	T9687	T9688	det	the,strain
R6144	T9688	T9686	pobj	strain,in
R6145	T9689	T9690	nummod	129,SvJ
R6146	T9690	T9688	compound	SvJ,strain
R6147	T9691	T9690	punct	/,SvJ
R6148	T9692	T9665	cc	and,maintained
R6149	T9693	T9665	conj	observed,maintained
R615	T1347	T1346	prep	of,response
R6150	T9694	T9695	det	a,phenotype
R6151	T9695	T9693	dobj	phenotype,observed
R6152	T9696	T9695	amod	similar,phenotype
R6153	T9697	T9665	punct	.,maintained
R6154	T9733	T9732	cc	and,Genotyping
R6155	T9734	T9735	compound	immunoblot,analyses
R6156	T9735	T9732	conj	analyses,Genotyping
R6157	T9736	T9735	punct	.,analyses
R6158	T9738	T9739	det	All,procedures
R6159	T9739	T9741	nsubjpass	procedures,performed
R616	T1348	T1349	nmod	bone,marrow
R6160	T9740	T9739	compound	mouse,procedures
R6161	T9742	T9741	auxpass	were,performed
R6162	T9743	T9741	prep	in,performed
R6163	T9744	T9743	pobj	accordance,in
R6164	T9745	T9744	prep	with,accordance
R6165	T9746	T9747	compound	McGill,University
R6166	T9747	T9748	compound	University,guidelines
R6167	T9748	T9745	pobj	guidelines,with
R6168	T9749	T9748	punct	", ",guidelines
R6169	T9750	T9751	dep	which,set
R617	T1349	T1350	nmod	marrow,cells
R6170	T9751	T9748	relcl	set,guidelines
R6171	T9752	T9751	auxpass	are,set
R6172	T9753	T9751	agent	by,set
R6173	T9754	T9755	det	the,Council
R6174	T9755	T9753	pobj	Council,by
R6175	T9756	T9755	compound	Canadian,Council
R6176	T9757	T9755	prep	on,Council
R6177	T9758	T9759	compound	Animal,Care
R6178	T9759	T9757	pobj	Care,on
R6179	T9760	T9741	punct	.,performed
R618	T1350	T1347	pobj	cells,of
R6180	T9762	T9763	amod	Genomic,DNA
R6181	T9763	T9764	nsubjpass	DNA,isolated
R6182	T9765	T9764	auxpass	was,isolated
R6183	T9766	T9764	prep	from,isolated
R6184	T9767	T9768	compound	tail,biopsies
R6185	T9768	T9766	pobj	biopsies,from
R6186	T9769	T9764	cc	and,isolated
R6187	T9770	T9764	conj	analyzed,isolated
R6188	T9771	T9770	prep	by,analyzed
R6189	T9772	T9773	compound	Southern,blotting
R619	T1351	T1350	amod	stromal,cells
R6190	T9773	T9771	pobj	blotting,by
R6191	T9774	T9773	cc	and,blotting
R6192	T9775	T9776	amod	genomic,analysis
R6193	T9776	T9773	conj	analysis,blotting
R6194	T9777	T9776	compound	PCR,analysis
R6195	T9778	T9764	punct	.,isolated
R6196	T9780	T9781	det	The,fragment
R6197	T9781	T9783	nsubjpass	fragment,amplified
R6198	T9782	T9781	compound	DNA,fragment
R6199	T9784	T9781	acl	utilized,fragment
R62	T301	T300	prep	of,generation
R620	T1352	T1346	prep	to,response
R6200	T9785	T9784	prep	as,utilized
R6201	T9786	T9787	det	the,probe
R6202	T9787	T9785	pobj	probe,as
R6203	T9788	T9787	prep	for,probe
R6204	T9789	T9790	det	the,analysis
R6205	T9790	T9788	pobj	analysis,for
R6206	T9791	T9792	compound	Southern,blotting
R6207	T9792	T9790	compound	blotting,analysis
R6208	T9793	T9783	auxpass	was,amplified
R6209	T9794	T9783	prep	with,amplified
R621	T1353	T1354	poss	their,microenvironment
R6210	T9795	T9796	det	the,oligonucleotides
R6211	T9796	T9794	pobj	oligonucleotides,with
R6212	T9797	T9796	amod	following,oligonucleotides
R6213	T9798	T9796	nummod	two,oligonucleotides
R6214	T9799	T9796	punct	(,oligonucleotides
R6215	T9800	T9796	appos	5,oligonucleotides
R6216	T9801	T9800	punct	′,5
R6217	T9802	T9800	punct	-,5
R6218	T9803	T9804	compound	AAG,T
R6219	T9804	T9800	appos	T,5
R622	T1354	T1352	pobj	microenvironment,to
R6220	T9805	T9804	compound	CCT,T
R6221	T9806	T9804	compound	TTA,T
R6222	T9807	T9804	compound	CTG,T
R6223	T9808	T9804	compound	GTT,T
R6224	T9809	T9804	compound	GTG,T
R6225	T9810	T9800	punct	-,5
R6226	T9811	T9800	nummod	3,5
R6227	T9812	T9800	punct	′,5
R6228	T9813	T9800	punct	),5
R6229	T9814	T9800	cc	and,5
R623	T1355	T1356	dep	that,favors
R6230	T9815	T9816	punct	(,5
R6231	T9816	T9800	conj	5,5
R6232	T9817	T9816	punct	′,5
R6233	T9818	T9816	punct	-,5
R6234	T9819	T9820	compound	CTT,CT
R6235	T9820	T9816	appos	CT,5
R6236	T9821	T9820	compound	GAA,CT
R6237	T9822	T9820	compound	ACG,CT
R6238	T9823	T9820	compound	CAC,CT
R6239	T9824	T9820	compound	CGT,CT
R624	T1356	T1346	relcl	favors,response
R6240	T9825	T9820	compound	AGG,CT
R6241	T9826	T9816	punct	-,5
R6242	T9827	T9816	nummod	3,5
R6243	T9828	T9816	punct	′,5
R6244	T9829	T9783	punct	),amplified
R6245	T9830	T9783	punct	.,amplified
R6246	T9832	T9833	det	The,allele
R6247	T9833	T9838	nsubjpass	allele,identified
R6248	T9834	T9835	amod	wild,type
R6249	T9835	T9833	compound	type,allele
R625	T1357	T1356	dobj	differentiation,favors
R6250	T9836	T9835	punct	-,type
R6251	T9837	T9833	compound	sam68,allele
R6252	T9839	T9838	auxpass	was,identified
R6253	T9840	T9838	prep	by,identified
R6254	T9841	T9842	amod	genomic,PCR
R6255	T9842	T9840	pobj	PCR,by
R6256	T9843	T9838	advcl	using,identified
R6257	T9844	T9845	det	the,oligonucleotides
R6258	T9845	T9843	dobj	oligonucleotides,using
R6259	T9846	T9845	amod	following,oligonucleotides
R626	T1358	T1357	prep	down,differentiation
R6260	T9847	T9845	appos	5,oligonucleotides
R6261	T9848	T9847	punct	′,5
R6262	T9849	T9847	punct	-,5
R6263	T9850	T9851	compound	AAA,AG
R6264	T9851	T9847	appos	AG,5
R6265	T9852	T9851	compound	TCC,AG
R6266	T9853	T9851	compound	TAA,AG
R6267	T9854	T9851	compound	CCC,AG
R6268	T9855	T9851	compound	TCC,AG
R6269	T9856	T9851	compound	TCA,AG
R627	T1359	T1360	det	the,lineage
R6270	T9857	T9851	compound	GTC,AG
R6271	T9858	T9847	punct	-,5
R6272	T9859	T9847	nummod	3,5
R6273	T9860	T9847	punct	′,5
R6274	T9861	T9847	cc	and,5
R6275	T9862	T9847	conj	5,5
R6276	T9863	T9862	punct	′,5
R6277	T9864	T9862	punct	-,5
R6278	T9865	T9866	compound	GAT,CAG
R6279	T9866	T9862	appos	CAG,5
R628	T1360	T1358	pobj	lineage,down
R6280	T9867	T9866	compound	ATG,CAG
R6281	T9868	T9866	compound	ATG,CAG
R6282	T9869	T9866	compound	GAT,CAG
R6283	T9870	T9866	compound	GAT,CAG
R6284	T9871	T9866	compound	ATC,CAG
R6285	T9872	T9866	compound	TGT,CAG
R6286	T9873	T9862	punct	-,5
R6287	T9874	T9862	nummod	3,5
R6288	T9875	T9862	punct	′,5
R6289	T9876	T9838	punct	.,identified
R629	T1361	T1360	compound	adipocyte,lineage
R6290	T9878	T9879	det	The,allele
R6291	T9879	T9883	nsubjpass	allele,identified
R6292	T9880	T9881	npadvmod	sam68,targeted
R6293	T9881	T9879	amod	targeted,allele
R6294	T9882	T9881	punct	-,targeted
R6295	T9884	T9883	auxpass	was,identified
R6296	T9885	T9883	prep	by,identified
R6297	T9886	T9887	amod	genomic,PCR
R6298	T9887	T9885	pobj	PCR,by
R6299	T9888	T9883	advcl	using,identified
R63	T302	T303	npadvmod	Sam68,null
R630	T1362	T1363	advmod	rather,than
R6300	T9889	T9890	det	the,oligonucleotides
R6301	T9890	T9888	dobj	oligonucleotides,using
R6302	T9891	T9890	amod	following,oligonucleotides
R6303	T9892	T9890	appos	5,oligonucleotides
R6304	T9893	T9892	punct	′,5
R6305	T9894	T9892	punct	-,5
R6306	T9895	T9896	compound	CTT,TCG
R6307	T9896	T9892	appos	TCG,5
R6308	T9897	T9896	compound	GGG,TCG
R6309	T9898	T9896	compound	TGG,TCG
R631	T1363	T1360	cc	than,lineage
R6310	T9899	T9896	compound	AGA,TCG
R6311	T9900	T9896	compound	GGC,TCG
R6312	T9901	T9896	compound	TAT,TCG
R6313	T9902	T9892	punct	-,5
R6314	T9903	T9892	nummod	3,5
R6315	T9904	T9892	punct	′,5
R6316	T9905	T9892	cc	and,5
R6317	T9906	T9892	conj	5,5
R6318	T9907	T9906	punct	′,5
R6319	T9908	T9906	punct	-,5
R632	T1364	T1365	det	the,lineage
R6320	T9909	T9910	compound	GTC,C
R6321	T9910	T9906	appos	C,5
R6322	T9911	T9910	compound	GGG,C
R6323	T9912	T9910	compound	CAT,C
R6324	T9913	T9910	compound	GCG,C
R6325	T9914	T9910	compound	CGC,C
R6326	T9915	T9910	compound	CTT,C
R6327	T9916	T9910	compound	GAG,C
R6328	T9917	T9906	punct	-,5
R6329	T9918	T9906	nummod	3,5
R633	T1365	T1360	conj	lineage,lineage
R6330	T9919	T9906	punct	′,5
R6331	T9920	T9883	punct	.,identified
R6332	T9979	T9978	cc	and,Radiology
R6333	T9980	T9981	compound	serum,biochemistry
R6334	T9981	T9978	conj	biochemistry,Radiology
R6335	T9982	T9978	prep	on,Radiology
R6336	T9983	T9984	nmod	Sam68,mice
R6337	T9984	T9982	pobj	mice,on
R6338	T9985	T9983	punct	+,Sam68
R6339	T9986	T9983	punct	/,Sam68
R634	T1366	T1365	compound	osteoblast,lineage
R6340	T9987	T9983	punct	+,Sam68
R6341	T9988	T9983	cc	and,Sam68
R6342	T9989	T9983	conj	Sam68,Sam68
R6343	T9990	T9989	punct	−,Sam68
R6344	T9991	T9989	punct	/,Sam68
R6345	T9992	T9989	punct	−,Sam68
R6346	T9993	T9978	punct	.,Radiology
R6347	T9995	T9996	nsubjpass	Radiography,performed
R6348	T9997	T9995	punct	", ",Radiography
R6349	T9998	T9995	conj	BMD,Radiography
R635	T1367	T1368	punct	[,22
R6350	T9999	T9998	punct	", ",BMD
R6351	T10000	T9998	cc	and,BMD
R6352	T10001	T9998	conj	micro-CT,BMD
R6353	T10002	T9996	auxpass	were,performed
R6354	T10003	T10004	advmod	essentially,described
R6355	T10004	T9996	advcl	described,performed
R6356	T10005	T10004	mark	as,described
R6357	T10006	T10004	advmod	previously,described
R6358	T10007	T10008	punct	[,63
R6359	T10008	T9996	parataxis	63,performed
R636	T1368	T1335	parataxis	22,attributed
R6360	T10009	T10008	punct	],63
R6361	T10010	T9996	punct	.,performed
R6362	T10012	T10013	nsubjpass	Mice,administered
R6363	T10014	T10013	auxpass	were,administered
R6364	T10015	T10016	det	a,dose
R6365	T10016	T10013	dobj	dose,administered
R6366	T10017	T10016	amod	lethal,dose
R6367	T10018	T10016	prep	of,dose
R6368	T10019	T10018	pobj	anesthetic,of
R6369	T10020	T10013	prep	at,administered
R637	T1369	T1368	punct	],22
R6370	T10021	T10022	det	the,times
R6371	T10022	T10020	pobj	times,at
R6372	T10023	T10022	amod	indicated,times
R6373	T10024	T10013	punct	", ",administered
R6374	T10025	T10013	conj	exsanguinated,administered
R6375	T10026	T10025	punct	", ",exsanguinated
R6376	T10027	T10025	cc	and,exsanguinated
R6377	T10028	T10025	conj	imaged,exsanguinated
R6378	T10029	T10028	advcl	using,imaged
R6379	T10030	T10031	det	a,MX20
R638	T1370	T1335	punct	.,attributed
R6380	T10031	T10029	dobj	MX20,using
R6381	T10032	T10031	compound	Faxitron,MX20
R6382	T10033	T10031	acl	equipped,MX20
R6383	T10034	T10033	prep	with,equipped
R6384	T10035	T10036	det	an,system
R6385	T10036	T10034	pobj	system,with
R6386	T10037	T10036	nmod	FPX,system
R6387	T10038	T10037	punct	-,FPX
R6388	T10039	T10037	nummod	2,FPX
R6389	T10040	T10036	compound	Imaging,system
R639	T1372	T1373	nsubjpass	Aging,associated
R6390	T10041	T10042	punct	(,Medoptics
R6391	T10042	T10031	parataxis	Medoptics,MX20
R6392	T10043	T10042	compound	Dalsa,Medoptics
R6393	T10044	T10042	punct	", ",Medoptics
R6394	T10045	T10042	npadvmod	Waterloo,Medoptics
R6395	T10046	T10042	punct	", ",Medoptics
R6396	T10047	T10042	npadvmod	Ontario,Medoptics
R6397	T10048	T10042	punct	", ",Medoptics
R6398	T10049	T10042	npadvmod	Canada,Medoptics
R6399	T10050	T10042	punct	),Medoptics
R64	T303	T305	amod	null,mice
R640	T1374	T1373	aux	has,associated
R6400	T10051	T10013	punct	.,administered
R6401	T10053	T10054	compound	Body,fat
R6402	T10054	T10055	nsubjpass	fat,evaluated
R6403	T10056	T10054	punct	", ",fat
R6404	T10057	T10054	conj	BMC,fat
R6405	T10058	T10057	punct	", ",BMC
R6406	T10059	T10057	cc	and,BMC
R6407	T10060	T10057	conj	BMD,BMC
R6408	T10061	T10055	auxpass	were,evaluated
R6409	T10062	T10055	advcl	using,evaluated
R641	T1375	T1373	advmod	long,associated
R6410	T10063	T10064	det	a,PixiMUS
R6411	T10064	T10062	dobj	PixiMUS,using
R6412	T10065	T10064	compound	Lunar,PixiMUS
R6413	T10066	T10064	nummod	1.46,PixiMUS
R6414	T10067	T10068	punct	(,Lunar
R6415	T10068	T10064	parataxis	Lunar,PixiMUS
R6416	T10069	T10068	compound	GE,Lunar
R6417	T10070	T10068	punct	-,Lunar
R6418	T10071	T10068	punct	", ",Lunar
R6419	T10072	T10068	npadvmod	Madison,Lunar
R642	T1376	T1373	auxpass	been,associated
R6420	T10073	T10068	punct	", ",Lunar
R6421	T10074	T10068	npadvmod	Wisconsin,Lunar
R6422	T10075	T10068	punct	", ",Lunar
R6423	T10076	T10077	compound	United,States
R6424	T10077	T10068	npadvmod	States,Lunar
R6425	T10078	T10068	punct	),Lunar
R6426	T10079	T10055	punct	.,evaluated
R6427	T10081	T10082	amod	Morphometric,parameters
R6428	T10082	T10083	nsubjpass	parameters,determined
R6429	T10084	T10083	auxpass	were,determined
R643	T1377	T1373	prep	with,associated
R6430	T10085	T10083	prep	on,determined
R6431	T10086	T10087	amod	anesthetized,mice
R6432	T10087	T10085	pobj	mice,on
R6433	T10088	T10083	prep	at,determined
R6434	T10089	T10090	det	the,time
R6435	T10090	T10088	pobj	time,at
R6436	T10091	T10090	prep	of,time
R6437	T10092	T10091	pobj	sacrifice,of
R6438	T10093	T10083	prep	by,determined
R6439	T10094	T10095	amod	direct,measurement
R644	T1378	T1379	det	an,increase
R6440	T10095	T10093	pobj	measurement,by
R6441	T10096	T10093	cc	or,by
R6442	T10097	T10093	conj	from,by
R6443	T10098	T10099	det	the,radiograph
R6444	T10099	T10097	pobj	radiograph,from
R6445	T10100	T10099	compound	Faxitron,radiograph
R6446	T10101	T10083	punct	.,determined
R6447	T10103	T10104	nsubjpass	Micro-CT,performed
R6448	T10105	T10104	auxpass	was,performed
R6449	T10106	T10104	prep	on,performed
R645	T1379	T1377	pobj	increase,with
R6450	T10107	T10108	det	the,femur
R6451	T10108	T10106	pobj	femur,on
R6452	T10109	T10108	amod	left,femur
R6453	T10110	T10108	cc	and,femur
R6454	T10111	T10112	amod	fourth,vertebra
R6455	T10112	T10108	conj	vertebra,femur
R6456	T10113	T10112	compound	lumbar,vertebra
R6457	T10114	T10104	prep	after,performed
R6458	T10115	T10114	pobj	removal,after
R6459	T10116	T10115	prep	of,removal
R646	T1380	T1379	prep	in,increase
R6460	T10117	T10118	amod	soft,tissues
R6461	T10118	T10116	pobj	tissues,of
R6462	T10119	T10115	cc	and,removal
R6463	T10120	T10121	amod	overnight,fixation
R6464	T10121	T10115	conj	fixation,removal
R6465	T10122	T10121	prep	in,fixation
R6466	T10123	T10124	nummod	4,%
R6467	T10124	T10125	compound	%,paraformaldehyde
R6468	T10125	T10122	pobj	paraformaldehyde,in
R6469	T10126	T10104	punct	.,performed
R647	T1381	T1382	compound	marrow,fat
R6470	T10128	T10129	det	The,metaphysis
R6471	T10129	T10131	nsubjpass	metaphysis,scanned
R6472	T10130	T10129	amod	distal,metaphysis
R6473	T10132	T10131	auxpass	was,scanned
R6474	T10133	T10131	prep	with,scanned
R6475	T10134	T10135	det	a,instrument
R6476	T10135	T10133	pobj	instrument,with
R6477	T10136	T10135	nmod	Skyscan,instrument
R6478	T10137	T10136	nummod	1072,Skyscan
R6479	T10138	T10135	compound	micro-CT,instrument
R648	T1382	T1380	pobj	fat,in
R6480	T10139	T10140	punct	(,Skyscan
R6481	T10140	T10135	parataxis	Skyscan,instrument
R6482	T10141	T10140	punct	", ",Skyscan
R6483	T10142	T10140	npadvmod	Antwerp,Skyscan
R6484	T10143	T10140	punct	", ",Skyscan
R6485	T10144	T10140	npadvmod	Belgium,Skyscan
R6486	T10145	T10140	punct	),Skyscan
R6487	T10146	T10131	punct	.,scanned
R6488	T10148	T10149	compound	Image,acquisition
R6489	T10149	T10150	nsubjpass	acquisition,performed
R649	T1383	T1382	punct	", ",fat
R6490	T10151	T10150	auxpass	was,performed
R6491	T10152	T10150	prep	at,performed
R6492	T10153	T10154	nummod	100,kV
R6493	T10154	T10152	pobj	kV,at
R6494	T10155	T10154	cc	and,kV
R6495	T10156	T10157	nummod	98,μA
R6496	T10157	T10154	conj	μA,kV
R6497	T10158	T10150	punct	", ",performed
R6498	T10159	T10150	prep	with,performed
R6499	T10160	T10161	det	a,rotation
R65	T304	T303	punct	-,null
R650	T1384	T1385	advmod	where,favored
R6500	T10161	T10159	pobj	rotation,with
R6501	T10162	T10163	nummod	0.9,°
R6502	T10163	T10161	compound	°,rotation
R6503	T10164	T10161	prep	between,rotation
R6504	T10165	T10164	pobj	frames,between
R6505	T10166	T10150	punct	.,performed
R6506	T10168	T10169	det	The,images
R6507	T10169	T10173	nsubjpass	images,used
R6508	T10170	T10171	advmod	two,dimensional
R6509	T10171	T10169	amod	dimensional,images
R651	T1385	T1382	relcl	favored,fat
R6510	T10172	T10171	punct	-,dimensional
R6511	T10174	T10173	auxpass	were,used
R6512	T10175	T10176	aux	to,generate
R6513	T10176	T10173	advcl	generate,used
R6514	T10177	T10178	advmod	three,dimensional
R6515	T10178	T10180	amod	dimensional,reconstructions
R6516	T10179	T10178	punct	-,dimensional
R6517	T10180	T10176	dobj	reconstructions,generate
R6518	T10181	T10182	aux	to,obtain
R6519	T10182	T10176	advcl	obtain,generate
R652	T1386	T1387	det	the,generation
R6520	T10183	T10184	amod	quantitative,data
R6521	T10184	T10182	dobj	data,obtain
R6522	T10185	T10182	prep	with,obtain
R6523	T10186	T10187	det	the,software
R6524	T10187	T10185	pobj	software,with
R6525	T10188	T10189	amod	3D,Creator
R6526	T10189	T10187	compound	Creator,software
R6527	T10190	T10187	acl	supplied,software
R6528	T10191	T10190	prep	with,supplied
R6529	T10192	T10193	det	the,instrument
R653	T1387	T1385	nsubjpass	generation,favored
R6530	T10193	T10191	pobj	instrument,with
R6531	T10194	T10173	punct	.,used
R6532	T10196	T10197	compound	Serum,biochemistry
R6533	T10197	T10198	nsubjpass	biochemistry,determined
R6534	T10199	T10200	punct	(,Mg
R6535	T10200	T10197	parataxis	Mg,biochemistry
R6536	T10201	T10200	nmod	ALP,Mg
R6537	T10202	T10200	punct	", ",Mg
R6538	T10203	T10200	nmod	Ca,Mg
R6539	T10204	T10200	punct	", ",Mg
R654	T1388	T1387	prep	of,generation
R6540	T10205	T10200	nmod	PO4,Mg
R6541	T10206	T10200	punct	", ",Mg
R6542	T10207	T10200	punct	),Mg
R6543	T10208	T10198	auxpass	was,determined
R6544	T10209	T10198	prep	at,determined
R6545	T10210	T10211	det	the,Lab
R6546	T10211	T10209	pobj	Lab,at
R6547	T10212	T10211	compound	Rodent,Lab
R6548	T10213	T10211	compound	Diagnostics,Lab
R6549	T10214	T10215	punct	(,University
R655	T1389	T1388	pobj	adipocytes,of
R6550	T10215	T10211	parataxis	University,Lab
R6551	T10216	T10215	compound	McGill,University
R6552	T10217	T10215	punct	", ",University
R6553	T10218	T10215	npadvmod	Montréal,University
R6554	T10219	T10215	punct	", ",University
R6555	T10220	T10215	npadvmod	Quebec,University
R6556	T10221	T10215	punct	", ",University
R6557	T10222	T10215	npadvmod	Canada,University
R6558	T10223	T10215	punct	),University
R6559	T10224	T10198	advcl	using,determined
R656	T1390	T1385	auxpass	is,favored
R6560	T10225	T10226	amod	routine,techniques
R6561	T10226	T10224	dobj	techniques,using
R6562	T10227	T10226	amod	automated,techniques
R6563	T10228	T10198	punct	.,determined
R6564	T10230	T10231	amod	Commercial,assays
R6565	T10231	T10232	nsubjpass	assays,used
R6566	T10233	T10232	auxpass	were,used
R6567	T10234	T10235	aux	to,determine
R6568	T10235	T10232	advcl	determine,used
R6569	T10236	T10237	compound	serum,levels
R657	T1391	T1385	prep	over,favored
R6570	T10237	T10235	dobj	levels,determine
R6571	T10238	T10237	prep	of,levels
R6572	T10239	T10238	pobj	CTX,of
R6573	T10240	T10241	punct	(,ELISA
R6574	T10241	T10239	parataxis	ELISA,CTX
R6575	T10242	T10241	compound	RatLaps,ELISA
R6576	T10243	T10241	punct	", ",ELISA
R6577	T10244	T10245	compound	Nordic,Bioscience
R6578	T10245	T10241	appos	Bioscience,ELISA
R6579	T10246	T10241	punct	),ELISA
R658	T1392	T1391	pobj	osteoblasts,over
R6580	T10247	T10232	punct	", ",used
R6581	T10248	T10249	nummod	17β,estradiol
R6582	T10249	T10232	dep	estradiol,used
R6583	T10250	T10251	punct	(,Immuno-Biological
R6584	T10251	T10249	parataxis	Immuno-Biological,estradiol
R6585	T10252	T10251	compound	IBL,Immuno-Biological
R6586	T10253	T10251	punct	", ",Immuno-Biological
R6587	T10254	T10251	npadvmod	Hamburg,Immuno-Biological
R6588	T10255	T10251	punct	", ",Immuno-Biological
R6589	T10256	T10251	npadvmod	Germany,Immuno-Biological
R659	T1393	T1394	punct	[,23
R6590	T10257	T10251	punct	),Immuno-Biological
R6591	T10258	T10249	punct	", ",estradiol
R6592	T10259	T10249	conj	leptin,estradiol
R6593	T10260	T10261	punct	(,Systems
R6594	T10261	T10259	parataxis	Systems,leptin
R6595	T10262	T10261	nmod	R,Systems
R6596	T10263	T10262	cc	&,R
R6597	T10264	T10262	conj	D,R
R6598	T10265	T10261	punct	", ",Systems
R6599	T10266	T10261	npadvmod	Minneapolis,Systems
R66	T305	T301	pobj	mice,of
R660	T1394	T1373	parataxis	23,associated
R6600	T10267	T10261	punct	", ",Systems
R6601	T10268	T10261	npadvmod	Minnesota,Systems
R6602	T10269	T10261	punct	", ",Systems
R6603	T10270	T10271	compound	United,States
R6604	T10271	T10261	npadvmod	States,Systems
R6605	T10272	T10261	punct	),Systems
R6606	T10273	T10259	punct	", ",leptin
R6607	T10274	T10259	cc	and,leptin
R6608	T10275	T10259	conj	interleukin,leptin
R6609	T10276	T10275	punct	-,interleukin
R661	T1395	T1394	punct	],23
R6610	T10277	T10275	nummod	6,interleukin
R6611	T10278	T10279	punct	(,Systems
R6612	T10279	T10275	parataxis	Systems,interleukin
R6613	T10280	T10279	nmod	R,Systems
R6614	T10281	T10280	cc	&,R
R6615	T10282	T10280	conj	D,R
R6616	T10283	T10279	punct	),Systems
R6617	T10284	T10232	punct	.,used
R6618	T10388	T10389	advmod	Ex,vivo
R6619	T10389	T10390	amod	vivo,assessment
R662	T1396	T1373	punct	.,associated
R6620	T10391	T10390	prep	of,assessment
R6621	T10392	T10393	nmod	osteoblast,activity
R6622	T10393	T10391	pobj	activity,of
R6623	T10394	T10392	cc	and,osteoblast
R6624	T10395	T10392	conj	osteoclast,osteoblast
R6625	T10396	T10390	punct	.,assessment
R6626	T10398	T10399	compound	Bone,marrow
R6627	T10399	T10400	nsubjpass	marrow,flushed
R6628	T10401	T10400	auxpass	was,flushed
R6629	T10402	T10400	prep	from,flushed
R663	T1398	T1399	nsubjpass	Osteoblasts,derived
R6630	T10403	T10404	det	the,tibia
R6631	T10404	T10402	pobj	tibia,from
R6632	T10405	T10404	cc	and,tibia
R6633	T10406	T10404	conj	femora,tibia
R6634	T10407	T10404	prep	of,tibia
R6635	T10408	T10409	amod	juvenile,mice
R6636	T10409	T10407	pobj	mice,of
R6637	T10410	T10411	nummod	4,week
R6638	T10411	T10413	npadvmod	week,old
R6639	T10412	T10411	punct	-,week
R664	T1400	T1398	cc	and,Osteoblasts
R6640	T10413	T10409	amod	old,mice
R6641	T10414	T10413	punct	-,old
R6642	T10415	T10409	nmod	Sam68,mice
R6643	T10416	T10415	punct	+,Sam68
R6644	T10417	T10415	punct	/,Sam68
R6645	T10418	T10415	punct	+,Sam68
R6646	T10419	T10415	cc	and,Sam68
R6647	T10420	T10415	conj	Sam68,Sam68
R6648	T10421	T10420	punct	−,Sam68
R6649	T10422	T10420	punct	/,Sam68
R665	T1401	T1398	conj	adipocytes,Osteoblasts
R6650	T10423	T10420	punct	−,Sam68
R6651	T10424	T10425	aux	to,obtain
R6652	T10425	T10400	advcl	obtain,flushed
R6653	T10426	T10427	amod	stromal,cells
R6654	T10427	T10425	dobj	cells,obtain
R6655	T10428	T10429	dep	that,maintained
R6656	T10429	T10427	relcl	maintained,cells
R6657	T10430	T10429	auxpass	were,maintained
R6658	T10431	T10429	prep	in,maintained
R6659	T10432	T10433	compound	differentiation,medium
R666	T1402	T1399	auxpass	are,derived
R6660	T10433	T10431	pobj	medium,in
R6661	T10434	T10400	prep	for,flushed
R6662	T10435	T10436	nummod	6,days
R6663	T10436	T10434	pobj	days,for
R6664	T10437	T10435	cc	or,6
R6665	T10438	T10435	conj	18,6
R6666	T10439	T10440	mark	as,described
R6667	T10440	T10400	advcl	described,flushed
R6668	T10441	T10442	punct	[,63
R6669	T10442	T10400	parataxis	63,flushed
R667	T1403	T1399	prep	from,derived
R6670	T10443	T10442	punct	],63
R6671	T10444	T10400	punct	.,flushed
R6672	T10446	T10447	nsubjpass	Cultures,stained
R6673	T10448	T10447	auxpass	were,stained
R6674	T10449	T10450	advmod	in,situ
R6675	T10450	T10447	advmod	situ,stained
R6676	T10451	T10447	prep	for,stained
R6677	T10452	T10453	compound	ALP,activity
R6678	T10453	T10451	pobj	activity,for
R6679	T10454	T10451	cc	and,for
R668	T1404	T1405	det	a,cell
R6680	T10455	T10451	conj	with,for
R6681	T10456	T10457	compound	von,Kossa
R6682	T10457	T10458	compound	Kossa,stain
R6683	T10458	T10455	pobj	stain,with
R6684	T10459	T10460	aux	to,detect
R6685	T10460	T10447	advcl	detect,stained
R6686	T10461	T10462	amod	mineralized,nodules
R6687	T10462	T10460	dobj	nodules,detect
R6688	T10463	T10447	punct	.,stained
R6689	T10465	T10466	amod	Total,RNA
R669	T1405	T1403	pobj	cell,from
R6690	T10466	T10467	nsubjpass	RNA,harvested
R6691	T10468	T10467	auxpass	was,harvested
R6692	T10469	T10467	prep	from,harvested
R6693	T10470	T10471	amod	parallel,cultures
R6694	T10471	T10469	pobj	cultures,from
R6695	T10472	T10467	prep	for,harvested
R6696	T10473	T10474	compound	RT,PCR
R6697	T10474	T10476	compound	PCR,analysis
R6698	T10475	T10474	punct	-,PCR
R6699	T10476	T10472	pobj	analysis,for
R67	T306	T300	prep	by,generation
R670	T1406	T1405	amod	common,cell
R6700	T10477	T10476	prep	of,analysis
R6701	T10478	T10479	npadvmod	osteoblast,related
R6702	T10479	T10481	amod	related,expression
R6703	T10480	T10479	punct	-,related
R6704	T10481	T10477	pobj	expression,of
R6705	T10482	T10481	compound	gene,expression
R6706	T10483	T10467	prep	over,harvested
R6707	T10484	T10483	pobj	time,over
R6708	T10485	T10486	mark	as,described
R6709	T10486	T10467	advcl	described,harvested
R671	T1407	T1405	amod	mesenchymal,cell
R6710	T10487	T10486	advmod	previously,described
R6711	T10488	T10489	punct	[,63
R6712	T10489	T10467	parataxis	63,harvested
R6713	T10490	T10489	punct	],63
R6714	T10491	T10467	punct	.,harvested
R6715	T10493	T10494	nsubj	Osteoclasts,isolated
R6716	T10495	T10494	aux	were,isolated
R6717	T10496	T10494	prep	from,isolated
R6718	T10497	T10498	det	the,bones
R6719	T10498	T10496	pobj	bones,from
R672	T1408	T1405	compound	precursor,cell
R6720	T10499	T10498	amod	crushed,bones
R6721	T10500	T10498	amod	long,bones
R6722	T10501	T10498	prep	of,bones
R6723	T10502	T10503	amod	juvenile,mice
R6724	T10503	T10501	pobj	mice,of
R6725	T10504	T10503	nmod	Sam68,mice
R6726	T10505	T10504	punct	+,Sam68
R6727	T10506	T10504	punct	/,Sam68
R6728	T10507	T10504	punct	+,Sam68
R6729	T10508	T10504	cc	and,Sam68
R673	T1409	T1405	relcl	present,cell
R6730	T10509	T10504	conj	Sam68,Sam68
R6731	T10510	T10509	punct	−,Sam68
R6732	T10511	T10509	punct	/,Sam68
R6733	T10512	T10509	punct	−,Sam68
R6734	T10513	T10494	cc	and,isolated
R6735	T10514	T10494	conj	used,isolated
R6736	T10515	T10514	prep	for,used
R6737	T10516	T10517	amod	quantitative,studies
R6738	T10517	T10515	pobj	studies,for
R6739	T10518	T10519	mark	as,described
R674	T1410	T1409	prep	in,present
R6740	T10519	T10494	advcl	described,isolated
R6741	T10520	T10521	punct	[,64
R6742	T10521	T10494	parataxis	64,isolated
R6743	T10522	T10521	punct	],64
R6744	T10523	T10494	punct	.,isolated
R6745	T10525	T10526	advmod	Briefly,plated
R6746	T10527	T10526	punct	", ",plated
R6747	T10528	T10526	nsubjpass	cells,plated
R6748	T10529	T10526	auxpass	were,plated
R6749	T10530	T10526	prep	on,plated
R675	T1411	T1412	compound	bone,marrow
R6750	T10531	T10532	compound	glass,coverslips
R6751	T10532	T10530	pobj	coverslips,on
R6752	T10533	T10530	cc	or,on
R6753	T10534	T10530	conj	on,on
R6754	T10535	T10536	compound	dentin,slices
R6755	T10536	T10534	pobj	slices,on
R6756	T10537	T10526	prep	in,plated
R6757	T10538	T10539	nummod	24,well
R6758	T10539	T10541	compound	well,plates
R6759	T10540	T10539	punct	-,well
R676	T1412	T1410	pobj	marrow,in
R6760	T10541	T10537	pobj	plates,in
R6761	T10542	T10541	compound	cluster,plates
R6762	T10543	T10526	prep	for,plated
R6763	T10544	T10543	pobj	assessment,for
R6764	T10545	T10544	prep	of,assessment
R6765	T10546	T10547	compound	cell,number
R6766	T10547	T10545	pobj	number,of
R6767	T10548	T10547	cc	and,number
R6768	T10549	T10550	compound	pit,number
R6769	T10550	T10547	conj	number,number
R677	T1413	T1399	punct	", ",derived
R6770	T10551	T10526	punct	", ",plated
R6771	T10552	T10526	advmod	respectively,plated
R6772	T10553	T10526	punct	.,plated
R6773	T10555	T10556	compound	Cover,slips
R6774	T10556	T10557	nsubjpass	slips,immersed
R6775	T10558	T10557	auxpass	were,immersed
R6776	T10559	T10557	prep	in,immersed
R6777	T10560	T10561	nummod	4,%
R6778	T10561	T10562	compound	%,paraformaldehyde
R6779	T10562	T10559	pobj	paraformaldehyde,in
R678	T1414	T1399	cc	and,derived
R6780	T10563	T10557	cc	and,immersed
R6781	T10564	T10557	conj	stained,immersed
R6782	T10565	T10564	prep	for,stained
R6783	T10566	T10567	compound	TRAP,activity
R6784	T10567	T10565	pobj	activity,for
R6785	T10568	T10564	prep	after,stained
R6786	T10569	T10570	nummod	2,h
R6787	T10570	T10568	pobj	h,after
R6788	T10571	T10557	punct	.,immersed
R6789	T10573	T10574	compound	Dentin,slices
R679	T1415	T1416	det	the,factors
R6790	T10574	T10575	nsubjpass	slices,left
R6791	T10576	T10575	auxpass	were,left
R6792	T10577	T10575	prep	for,left
R6793	T10578	T10579	nummod	28,h
R6794	T10579	T10577	pobj	h,for
R6795	T10580	T10575	prep	before,left
R6796	T10581	T10580	pcomp	removing,before
R6797	T10582	T10583	det	the,cells
R6798	T10583	T10581	dobj	cells,removing
R6799	T10584	T10581	prep	with,removing
R68	T307	T308	amod	homologous,recombination
R680	T1416	T1417	nsubjpass	factors,understood
R6800	T10585	T10586	nummod	1,M
R6801	T10586	T10587	compound	M,hydroxide
R6802	T10587	T10584	pobj	hydroxide,with
R6803	T10588	T10587	compound	ammonium,hydroxide
R6804	T10589	T10581	cc	and,removing
R6805	T10590	T10591	dep	air,drying
R6806	T10591	T10581	conj	drying,removing
R6807	T10592	T10591	prep	before,drying
R6808	T10593	T10592	pobj	coating,before
R6809	T10594	T10593	prep	with,coating
R681	T1417	T1399	conj	understood,derived
R6810	T10595	T10596	compound	sputter,Pd
R6811	T10596	T10594	pobj	Pd,with
R6812	T10597	T10596	compound	Au,Pd
R6813	T10598	T10596	punct	-,Pd
R6814	T10599	T10593	cc	and,coating
R6815	T10600	T10593	conj	examination,coating
R6816	T10601	T10600	prep	with,examination
R6817	T10602	T10603	amod	scanning,microscopy
R6818	T10603	T10601	pobj	microscopy,with
R6819	T10604	T10603	compound	electron,microscopy
R682	T1418	T1419	dep	that,control
R6820	T10605	T10606	punct	(,Centre
R6821	T10606	T10603	parataxis	Centre,microscopy
R6822	T10607	T10606	compound	McGill,Centre
R6823	T10608	T10609	compound	Electron,Microscopy
R6824	T10609	T10606	compound	Microscopy,Centre
R6825	T10610	T10606	punct	),Centre
R6826	T10611	T10575	punct	.,left
R6827	T10613	T10614	compound	Light,microscope
R6828	T10614	T10615	compound	microscope,images
R6829	T10615	T10616	nsubjpass	images,captured
R683	T1419	T1416	relcl	control,factors
R6830	T10617	T10616	auxpass	were,captured
R6831	T10618	T10616	advcl	using,captured
R6832	T10619	T10620	det	a,microscope
R6833	T10620	T10618	dobj	microscope,using
R6834	T10621	T10622	compound	Leica,DMR
R6835	T10622	T10620	compound	DMR,microscope
R6836	T10623	T10620	acl	equipped,microscope
R6837	T10624	T10623	prep	with,equipped
R6838	T10625	T10626	det	a,camera
R6839	T10626	T10624	pobj	camera,with
R684	T1420	T1421	det	this,switch
R6840	T10627	T10626	nmod	Retiga,camera
R6841	T10628	T10627	nummod	1300,Retiga
R6842	T10629	T10616	punct	.,captured
R6843	T10631	T10632	amod	Quantitative,analyses
R6844	T10632	T10633	nsubjpass	analyses,performed
R6845	T10634	T10633	auxpass	were,performed
R6846	T10635	T10633	advcl	using,performed
R6847	T10636	T10637	compound	Adobe,Photoshop
R6848	T10637	T10635	dobj	Photoshop,using
R6849	T10638	T10637	cc	and,Photoshop
R685	T1421	T1419	dobj	switch,control
R6850	T10639	T10640	det	the,data
R6851	T10640	T10637	conj	data,Photoshop
R6852	T10641	T10640	acl	presented,data
R6853	T10642	T10641	prep	as,presented
R6854	T10643	T10644	det	the,SD
R6855	T10644	T10642	pobj	SD,as
R6856	T10645	T10644	nmod	mean,SD
R6857	T10646	T10644	punct	±,SD
R6858	T10647	T10644	prep	of,SD
R6859	T10648	T10649	nummod	two,experiments
R686	T1422	T1423	npadvmod	age,induced
R6860	T10649	T10647	pobj	experiments,of
R6861	T10650	T10648	cc	or,two
R6862	T10651	T10648	conj	three,two
R6863	T10652	T10649	amod	independent,experiments
R6864	T10653	T10633	punct	.,performed
R6865	T10655	T10656	amod	Statistical,comparisons
R6866	T10656	T10657	nsubjpass	comparisons,made
R6867	T10658	T10657	auxpass	were,made
R6868	T10659	T10657	advcl	using,made
R6869	T10660	T10661	det	the,Student
R687	T1423	T1421	amod	induced,switch
R6870	T10661	T10662	poss	Student,test
R6871	T10662	T10659	dobj	test,using
R6872	T10663	T10661	case	's,Student
R6873	T10664	T10662	compound	t,test
R6874	T10665	T10657	punct	.,made
R6875	T10694	T10693	prep	of,Preparation
R6876	T10695	T10696	nmod	mouse,fibroblast
R6877	T10696	T10694	pobj	fibroblast,of
R6878	T10697	T10696	amod	embryonic,fibroblast
R6879	T10698	T10693	cc	and,Preparation
R688	T1424	T1423	punct	-,induced
R6880	T10699	T10693	conj	induction,Preparation
R6881	T10700	T10699	prep	of,induction
R6882	T10701	T10702	compound	adipocyte,differentiation
R6883	T10702	T10700	pobj	differentiation,of
R6884	T10703	T10699	punct	.,induction
R6885	T10705	T10706	nsubjpass	MEFs,prepared
R6886	T10707	T10706	auxpass	were,prepared
R6887	T10708	T10706	prep	from,prepared
R6888	T10709	T10710	nummod	14.5,day
R6889	T10710	T10712	npadvmod	day,old
R689	T1425	T1421	prep	toward,switch
R6890	T10711	T10710	punct	-,day
R6891	T10712	T10714	amod	old,embryos
R6892	T10713	T10712	punct	-,old
R6893	T10714	T10708	pobj	embryos,from
R6894	T10715	T10706	punct	.,prepared
R6895	T10717	T10718	advmod	Only,MEFs
R6896	T10718	T10722	nsubjpass	MEFs,used
R6897	T10719	T10720	amod	early,passage
R6898	T10720	T10718	compound	passage,MEFs
R6899	T10721	T10720	punct	-,passage
R69	T308	T306	pobj	recombination,by
R690	T1426	T1427	amod	adipogenic,differentiation
R6900	T10723	T10722	auxpass	were,used
R6901	T10724	T10722	prep	for,used
R6902	T10725	T10726	det	the,studies
R6903	T10726	T10724	pobj	studies,for
R6904	T10727	T10726	amod	experimental,studies
R6905	T10728	T10722	punct	", ",used
R6906	T10729	T10722	cc	and,used
R6907	T10730	T10731	nsubjpass	induction,carried
R6908	T10731	T10722	conj	carried,used
R6909	T10732	T10730	prep	of,induction
R691	T1427	T1425	pobj	differentiation,toward
R6910	T10733	T10734	compound	adipocyte,differentiation
R6911	T10734	T10732	pobj	differentiation,of
R6912	T10735	T10731	auxpass	was,carried
R6913	T10736	T10731	prt	out,carried
R6914	T10737	T10731	prep	according,carried
R6915	T10738	T10737	prep	to,according
R6916	T10739	T10740	det	the,methods
R6917	T10740	T10738	pobj	methods,to
R6918	T10741	T10742	advmod	previously,described
R6919	T10742	T10740	acl	described,methods
R692	T1428	T1417	auxpass	is,understood
R6920	T10743	T10744	punct	[,30
R6921	T10744	T10731	parataxis	30,carried
R6922	T10745	T10744	punct	],30
R6923	T10746	T10731	punct	.,carried
R6924	T10748	T10749	amod	Cultured,cells
R6925	T10749	T10750	nsubjpass	cells,stained
R6926	T10751	T10750	auxpass	were,stained
R6927	T10752	T10750	prep	with,stained
R6928	T10753	T10754	compound	Oil,O
R6929	T10754	T10752	pobj	O,with
R693	T1429	T1417	neg	not,understood
R6930	T10755	T10754	compound	Red,O
R6931	T10756	T10757	mark	as,described
R6932	T10757	T10750	advcl	described,stained
R6933	T10758	T10759	punct	[,29
R6934	T10759	T10750	parataxis	29,stained
R6935	T10760	T10759	punct	],29
R6936	T10761	T10750	punct	.,stained
R6937	T10818	T10819	compound	RNA,preparation
R6938	T10820	T10819	cc	and,preparation
R6939	T10821	T10822	compound	RT,PCR
R694	T1430	T1417	advmod	well,understood
R6940	T10822	T10819	conj	PCR,preparation
R6941	T10823	T10822	punct	-,PCR
R6942	T10824	T10825	aux	to,assess
R6943	T10825	T10819	advcl	assess,preparation
R6944	T10826	T10825	dobj	adipogenesis,assess
R6945	T10827	T10825	prep	in,assess
R6946	T10828	T10829	nmod	Sam68,MEFs
R6947	T10829	T10827	pobj	MEFs,in
R6948	T10830	T10828	punct	−,Sam68
R6949	T10831	T10828	punct	/,Sam68
R695	T1431	T1432	punct	[,24
R6950	T10832	T10828	punct	−,Sam68
R6951	T10833	T10819	punct	.,preparation
R6952	T10835	T10836	amod	Total,RNA
R6953	T10836	T10838	nsubjpass	RNA,prepared
R6954	T10837	T10836	amod	cellular,RNA
R6955	T10839	T10838	auxpass	was,prepared
R6956	T10840	T10838	prep	by,prepared
R6957	T10841	T10842	compound	TRIzol,reagent
R6958	T10842	T10840	pobj	reagent,by
R6959	T10843	T10838	prep	according,prepared
R696	T1432	T1417	parataxis	24,understood
R6960	T10844	T10843	prep	to,according
R6961	T10845	T10846	det	the,manufacturer
R6962	T10846	T10847	poss	manufacturer,protocol
R6963	T10847	T10844	pobj	protocol,to
R6964	T10848	T10846	case	's,manufacturer
R6965	T10849	T10850	punct	(,Invitrogen
R6966	T10850	T10847	parataxis	Invitrogen,protocol
R6967	T10851	T10850	punct	", ",Invitrogen
R6968	T10852	T10850	npadvmod	Carlsbad,Invitrogen
R6969	T10853	T10850	punct	", ",Invitrogen
R697	T1433	T1432	punct	],24
R6970	T10854	T10850	npadvmod	California,Invitrogen
R6971	T10855	T10850	punct	", ",Invitrogen
R6972	T10856	T10857	compound	United,States
R6973	T10857	T10850	npadvmod	States,Invitrogen
R6974	T10858	T10850	punct	),Invitrogen
R6975	T10859	T10838	punct	.,prepared
R6976	T10861	T10862	amod	Total,RNA
R6977	T10862	T10863	nsubjpass	RNA,transcribed
R6978	T10864	T10865	punct	(,μg
R6979	T10865	T10862	parataxis	μg,RNA
R698	T1434	T1417	punct	.,understood
R6980	T10866	T10865	nummod	1,μg
R6981	T10867	T10865	punct	),μg
R6982	T10868	T10863	auxpass	was,transcribed
R6983	T10869	T10863	advmod	reverse,transcribed
R6984	T10870	T10863	punct	-,transcribed
R6985	T10871	T10863	punct	", ",transcribed
R6986	T10872	T10863	cc	and,transcribed
R6987	T10873	T10874	compound	cDNA,samples
R6988	T10874	T10875	nsubjpass	samples,subjected
R6989	T10875	T10863	conj	subjected,transcribed
R699	T1436	T1437	mark	While,associated
R6990	T10876	T10875	auxpass	were,subjected
R6991	T10877	T10875	prep	to,subjected
R6992	T10878	T10877	pobj	PCR,to
R6993	T10879	T10875	punct	.,subjected
R6994	T10881	T10882	compound	RT,PCR
R6995	T10882	T10884	nsubjpass	PCR,normalized
R6996	T10883	T10882	punct	-,PCR
R6997	T10885	T10884	auxpass	was,normalized
R6998	T10886	T10884	agent	by,normalized
R6999	T10887	T10888	det	the,level
R7	T241	T239	pobj	Protein,of
R70	T309	T297	punct	.,report
R700	T1437	T1444	advcl	associated,remains
R7000	T10888	T10886	pobj	level,by
R7001	T10889	T10888	amod	transcriptional,level
R7002	T10890	T10888	prep	of,level
R7003	T10891	T10890	pobj	GAPDH,of
R7004	T10892	T10884	punct	.,normalized
R7005	T10894	T10895	det	The,primers
R7006	T10895	T10902	nsubjpass	primers,used
R7007	T10896	T10895	amod	following,primers
R7008	T10897	T10895	nummod	5,primers
R7009	T10898	T10897	punct	′,5
R701	T1438	T1439	amod	several,proteins
R7010	T10899	T10897	cc	and,5
R7011	T10900	T10897	conj	3,5
R7012	T10901	T10900	punct	′,3
R7013	T10903	T10902	auxpass	were,used
R7014	T10904	T10905	aux	to,evaluate
R7015	T10905	T10902	advcl	evaluate,used
R7016	T10906	T10907	amod	adipogenic,differentiation
R7017	T10907	T10905	dobj	differentiation,evaluate
R7018	T10908	T10902	punct	: ,used
R7019	T10909	T10902	dep	KLF5,used
R702	T1439	T1437	nsubjpass	proteins,associated
R7020	T10910	T10909	punct	: ,KLF5
R7021	T10911	T10909	appos	5,KLF5
R7022	T10912	T10911	punct	',5
R7023	T10913	T10911	punct	-,5
R7024	T10914	T10915	compound	AGA,C
R7025	T10915	T10911	appos	C,5
R7026	T10916	T10915	compound	CAA,C
R7027	T10917	T10915	compound	GCT,C
R7028	T10918	T10915	compound	GAG,C
R7029	T10919	T10915	compound	ATG,C
R703	T1440	T1439	amod	transcriptional,proteins
R7030	T10920	T10915	compound	CTG,C
R7031	T10921	T10911	punct	-,5
R7032	T10922	T10911	nummod	3,5
R7033	T10923	T10911	punct	′,5
R7034	T10924	T10911	punct	", ",5
R7035	T10925	T10911	appos	5,5
R7036	T10926	T10925	punct	′,5
R7037	T10927	T10925	punct	-,5
R7038	T10928	T10929	compound	GGC,GC
R7039	T10929	T10925	appos	GC,5
R704	T1441	T1439	amod	regulatory,proteins
R7040	T10930	T10929	compound	AAA,GC
R7041	T10931	T10929	compound	CCT,GC
R7042	T10932	T10929	compound	CCA,GC
R7043	T10933	T10929	compound	GTC,GC
R7044	T10934	T10925	punct	-,5
R7045	T10935	T10925	nummod	3,5
R7046	T10936	T10925	punct	′,5
R7047	T10937	T10909	punct	;,KLF5
R7048	T10938	T10909	conj	PPARγ,KLF5
R7049	T10939	T10938	punct	: ,PPARγ
R705	T1442	T1437	aux	have,associated
R7050	T10940	T10938	appos	5,PPARγ
R7051	T10941	T10940	punct	′,5
R7052	T10942	T10940	punct	-,5
R7053	T10943	T10944	compound	GTG,C
R7054	T10944	T10940	appos	C,5
R7055	T10945	T10944	compound	CGA,C
R7056	T10946	T10944	compound	TCA,C
R7057	T10947	T10944	compound	AAG,C
R7058	T10948	T10944	compound	TAG,C
R7059	T10949	T10944	compound	AAC,C
R706	T1443	T1437	auxpass	been,associated
R7060	T10950	T10944	compound	CTG,C
R7061	T10951	T10940	punct	-,5
R7062	T10952	T10940	nummod	3,5
R7063	T10953	T10940	punct	′,5
R7064	T10954	T10940	punct	", ",5
R7065	T10955	T10940	appos	5,5
R7066	T10956	T10955	punct	′,5
R7067	T10957	T10955	punct	-,5
R7068	T10958	T10959	compound	CCT,TCG
R7069	T10959	T10955	appos	TCG,5
R707	T1445	T1437	prep	with,associated
R7070	T10960	T10959	compound	ATC,TCG
R7071	T10961	T10959	compound	ATA,TCG
R7072	T10962	T10959	compound	AAT,TCG
R7073	T10963	T10959	compound	AAG,TCG
R7074	T10964	T10959	compound	CTT,TCG
R7075	T10965	T10959	compound	CAA,TCG
R7076	T10966	T10955	punct	-,5
R7077	T10967	T10955	nummod	3,5
R7078	T10968	T10955	punct	′,5
R7079	T10969	T10938	punct	;,PPARγ
R708	T1446	T1447	compound	cell,determination
R7080	T10970	T10971	compound	β,Actin
R7081	T10971	T10938	conj	Actin,PPARγ
R7082	T10972	T10971	punct	-,Actin
R7083	T10973	T10971	punct	: ,Actin
R7084	T10974	T10971	appos	5,Actin
R7085	T10975	T10974	punct	′,5
R7086	T10976	T10974	punct	-,5
R7087	T10977	T10978	compound	TAG,GC
R7088	T10978	T10974	appos	GC,5
R7089	T10979	T10978	compound	GCG,GC
R709	T1447	T1445	pobj	determination,with
R7090	T10980	T10978	compound	GAC,GC
R7091	T10981	T10978	compound	TGT,GC
R7092	T10982	T10978	compound	TAC,GC
R7093	T10983	T10978	compound	TGA,GC
R7094	T10984	T10974	punct	-,5
R7095	T10985	T10974	nummod	3,5
R7096	T10986	T10974	punct	′,5
R7097	T10987	T10974	punct	", ",5
R7098	T10988	T10974	appos	5,5
R7099	T10989	T10988	punct	′,5
R71	T311	T312	amod	Aged,mice
R710	T1448	T1447	compound	fate,determination
R7100	T10990	T10988	punct	-,5
R7101	T10991	T10992	compound	AGC,TGG
R7102	T10992	T10988	appos	TGG,5
R7103	T10993	T10992	compound	CTT,TGG
R7104	T10994	T10992	compound	CAT,TGG
R7105	T10995	T10992	compound	ACA,TGG
R7106	T10996	T10992	compound	TCA,TGG
R7107	T10997	T10992	compound	AGT,TGG
R7108	T10998	T10988	punct	-,5
R7109	T10999	T10988	nummod	3,5
R711	T1449	T1447	prep	of,determination
R7110	T11000	T10988	punct	′,5
R7111	T11001	T10971	punct	;,Actin
R7112	T11002	T10971	cc	and,Actin
R7113	T11003	T10971	conj	Sam68,Actin
R7114	T11004	T11003	punct	: ,Sam68
R7115	T11005	T11003	appos	5,Sam68
R7116	T11006	T11005	punct	′,5
R7117	T11007	T11005	punct	-,5
R7118	T11008	T11009	compound	GTG,A
R7119	T11009	T11005	appos	A,5
R712	T1450	T1451	nmod	bone,marrow
R7120	T11010	T11009	compound	GAG,A
R7121	T11011	T11009	compound	ACC,A
R7122	T11012	T11009	compound	CCA,A
R7123	T11013	T11009	compound	AAT,A
R7124	T11014	T11009	compound	ATG,A
R7125	T11015	T11009	compound	CCC,A
R7126	T11016	T11005	punct	-,5
R7127	T11017	T11005	nummod	3,5
R7128	T11018	T11005	punct	′,5
R7129	T11019	T11005	cc	and,5
R713	T1451	T1452	nmod	marrow,cells
R7130	T11020	T11005	conj	5,5
R7131	T11021	T11020	punct	′,5
R7132	T11022	T11020	punct	-,5
R7133	T11023	T11024	compound	AAA,A
R7134	T11024	T11020	appos	A,5
R7135	T11025	T11024	compound	CTG,A
R7136	T11026	T11024	compound	CTC,A
R7137	T11027	T11024	compound	CTG,A
R7138	T11028	T11024	compound	ACA,A
R7139	T11029	T11024	compound	GAT,A
R714	T1452	T1449	pobj	cells,of
R7140	T11030	T11024	compound	ATC,A
R7141	T11031	T11020	punct	-,5
R7142	T11032	T11020	nummod	3,5
R7143	T11033	T11020	punct	′,5
R7144	T11034	T10902	punct	.,used
R7145	T11076	T11077	nmod	Sam68,regulation
R7146	T11078	T11077	advmod	down,regulation
R7147	T11079	T11077	punct	-,regulation
R7148	T11080	T11077	acl	using,regulation
R7149	T11081	T11080	dobj	Sam68sh,using
R715	T1453	T1452	amod	mesenchymal,cells
R7150	T11082	T11077	punct	.,regulation
R7151	T11084	T11085	aux	To,generate
R7152	T11085	T11086	advcl	generate,purchased
R7153	T11087	T11088	det	an,shRNA
R7154	T11088	T11085	dobj	shRNA,generate
R7155	T11089	T11086	punct	", ",purchased
R7156	T11090	T11091	nummod	64,base
R7157	T11091	T11093	compound	base,nucleotides
R7158	T11092	T11091	punct	-,base
R7159	T11093	T11086	nsubjpass	nucleotides,purchased
R716	T1454	T1452	punct	", ",cells
R7160	T11094	T11093	compound	duplex,nucleotides
R7161	T11095	T11093	compound	DNA,nucleotides
R7162	T11096	T11086	auxpass	were,purchased
R7163	T11097	T11086	prep	from,purchased
R7164	T11098	T11097	pobj	Invitrogen,from
R7165	T11099	T11086	punct	.,purchased
R7166	T11101	T11102	det	This,fragment
R7167	T11102	T11103	nsubj	fragment,comprised
R7168	T11104	T11105	nummod	19,residues
R7169	T11105	T11103	dobj	residues,comprised
R717	T1455	T1452	prep	including,cells
R7170	T11106	T11105	compound	base,residues
R7171	T11107	T11105	amod	specific,residues
R7172	T11108	T11107	prep	to,specific
R7173	T11109	T11110	compound	mouse,Sam68
R7174	T11110	T11108	pobj	Sam68,to
R7175	T11111	T11112	punct	(,TTTTTGGAAA
R7176	T11112	T11103	parataxis	TTTTTGGAAA,comprised
R7177	T11113	T11112	compound	GATCCCC,TTTTTGGAAA
R7178	T11114	T11112	compound	AAGATGACGAGGAGAATTATTCAAGAGA,TTTTTGGAAA
R7179	T11115	T11112	compound	TAATTCTCCTCGTCATCTT,TTTTTGGAAA
R718	T1456	T1457	nmod	peroxisome,receptor
R7180	T11116	T11112	punct	),TTTTTGGAAA
R7181	T11117	T11103	punct	.,comprised
R7182	T11119	T11120	det	This,fragment
R7183	T11120	T11121	nsubjpass	fragment,cloned
R7184	T11122	T11121	auxpass	was,cloned
R7185	T11123	T11121	prep	into,cloned
R7186	T11124	T11125	compound	pSUPER,retro
R7187	T11125	T11123	pobj	retro,into
R7188	T11126	T11127	punct	(,OligoEngine
R7189	T11127	T11125	parataxis	OligoEngine,retro
R719	T1457	T1455	pobj	receptor,including
R7190	T11128	T11127	punct	", ",OligoEngine
R7191	T11129	T11127	npadvmod	Seattle,OligoEngine
R7192	T11130	T11127	punct	", ",OligoEngine
R7193	T11131	T11127	npadvmod	Washington,OligoEngine
R7194	T11132	T11127	punct	", ",OligoEngine
R7195	T11133	T11134	compound	United,States
R7196	T11134	T11127	npadvmod	States,OligoEngine
R7197	T11135	T11127	punct	),OligoEngine
R7198	T11136	T11121	punct	.,cloned
R7199	T11138	T11139	nsubjpass	Transfections,performed
R72	T312	T317	nsubj	mice,preserved
R720	T1458	T1459	npadvmod	proliferator,activated
R7200	T11140	T11139	auxpass	were,performed
R7201	T11141	T11139	advcl	using,performed
R7202	T11142	T11143	compound	LipofectAMINE,Plus
R7203	T11143	T11141	dobj	Plus,using
R7204	T11144	T11145	punct	(,Invitrogen
R7205	T11145	T11143	parataxis	Invitrogen,Plus
R7206	T11146	T11145	punct	),Invitrogen
R7207	T11147	T11141	prep	with,using
R7208	T11148	T11149	amod	cloned,fragment
R7209	T11149	T11147	pobj	fragment,with
R721	T1459	T1457	amod	activated,receptor
R7210	T11150	T11149	compound	DNA,fragment
R7211	T11151	T11149	cc	or,fragment
R7212	T11152	T11153	amod	empty,vector
R7213	T11153	T11149	conj	vector,fragment
R7214	T11154	T11139	punct	.,performed
R7215	T11156	T11157	prep	At,selected
R7216	T11158	T11159	nummod	48,h
R7217	T11159	T11156	pobj	h,At
R7218	T11160	T11159	prep	after,h
R7219	T11161	T11160	pobj	transfection,after
R722	T1460	T1459	punct	–,activated
R7220	T11162	T11157	punct	", ",selected
R7221	T11163	T11157	nsubjpass	populations,selected
R7222	T11164	T11157	auxpass	were,selected
R7223	T11165	T11157	prep	for,selected
R7224	T11166	T11167	nummod	2.5,μg
R7225	T11167	T11168	nmod	μg,puromycin
R7226	T11168	T11165	pobj	puromycin,for
R7227	T11169	T11170	punct	/,ml
R7228	T11170	T11167	prep	ml,μg
R7229	T11171	T11157	cc	and,selected
R723	T1461	T1457	punct	γ,receptor
R7230	T11172	T11173	det	the,knockdown
R7231	T11173	T11174	nsubj	knockdown,observed
R7232	T11174	T11157	conj	observed,selected
R7233	T11175	T11174	prep	by,observed
R7234	T11176	T11175	pcomp	immunoblotting,by
R7235	T11177	T11176	prep	with,immunoblotting
R7236	T11178	T11179	nmod	anti-Sam68,antibody
R7237	T11179	T11177	pobj	antibody,with
R7238	T11180	T11181	punct	(,AD1
R7239	T11181	T11178	parataxis	AD1,anti-Sam68
R724	T1462	T1457	punct	(,receptor
R7240	T11182	T11181	punct	),AD1
R7241	T11183	T11157	punct	.,selected
R7242	T11232	T11233	amod	Osteogenic,analysis
R7243	T11234	T11233	compound	differentiation,analysis
R7244	T11235	T11233	prep	of,analysis
R7245	T11236	T11237	nmod	C3HT101,cells
R7246	T11237	T11235	pobj	cells,of
R7247	T11238	T11236	punct	/,C3HT101
R7248	T11239	T11236	nummod	2,C3HT101
R7249	T11240	T11233	punct	.,analysis
R725	T1463	T1457	appos	PPARγ,receptor
R7250	T11242	T11243	prep	For,incubated
R7251	T11244	T11245	amod	osteogenic,differentiation
R7252	T11245	T11242	pobj	differentiation,For
R7253	T11246	T11243	punct	", ",incubated
R7254	T11247	T11248	nmod	C3HT101,cells
R7255	T11248	T11243	nsubjpass	cells,incubated
R7256	T11249	T11247	punct	/,C3HT101
R7257	T11250	T11247	nummod	2,C3HT101
R7258	T11251	T11252	punct	(,ATCC
R7259	T11252	T11247	parataxis	ATCC,C3HT101
R726	T1464	T1457	punct	),receptor
R7260	T11253	T11252	punct	),ATCC
R7261	T11254	T11243	auxpass	were,incubated
R7262	T11255	T11243	prep	in,incubated
R7263	T11256	T11257	nummod	48,well
R7264	T11257	T11259	compound	well,plates
R7265	T11258	T11257	punct	-,well
R7266	T11259	T11255	pobj	plates,in
R7267	T11260	T11259	compound	tissue,plates
R7268	T11261	T11259	compound	culture,plates
R7269	T11262	T11243	prep	at,incubated
R727	T1465	T1457	cc	and,receptor
R7270	T11263	T11264	quantmod	1.25,104
R7271	T11264	T11266	nummod	104,cells
R7272	T11265	T11264	punct	×,104
R7273	T11266	T11262	pobj	cells,at
R7274	T11267	T11268	punct	/,cm2
R7275	T11268	T11266	prep	cm2,cells
R7276	T11269	T11243	punct	.,incubated
R7277	T11271	T11272	prep	After,changed
R7278	T11273	T11274	nummod	24,h
R7279	T11274	T11276	compound	h,incubation
R728	T1466	T1457	conj	KLF5,receptor
R7280	T11275	T11274	punct	-,h
R7281	T11276	T11271	pobj	incubation,After
R7282	T11277	T11272	punct	", ",changed
R7283	T11278	T11279	det	the,media
R7284	T11279	T11272	nsubjpass	media,changed
R7285	T11280	T11279	compound	culture,media
R7286	T11281	T11272	auxpass	were,changed
R7287	T11282	T11272	prep	to,changed
R7288	T11283	T11284	amod	fresh,media
R7289	T11284	T11282	pobj	media,to
R729	T1467	T1468	punct	[,25
R7290	T11285	T11284	acl	containing,media
R7291	T11286	T11287	nummod	300,ng
R7292	T11287	T11288	nmod	ng,BMP
R7293	T11288	T11285	dobj	BMP,containing
R7294	T11289	T11290	punct	/,ml
R7295	T11290	T11287	prep	ml,ng
R7296	T11291	T11288	punct	-,BMP
R7297	T11292	T11288	nummod	2,BMP
R7298	T11293	T11294	punct	(,Sigma
R7299	T11294	T11288	parataxis	Sigma,BMP
R73	T313	T312	nmod	Sam68,mice
R730	T1468	T1437	parataxis	25,associated
R7300	T11295	T11294	punct	", ",Sigma
R7301	T11296	T11297	compound	St.,Louis
R7302	T11297	T11294	npadvmod	Louis,Sigma
R7303	T11298	T11294	punct	", ",Sigma
R7304	T11299	T11294	npadvmod	Missouri,Sigma
R7305	T11300	T11294	punct	", ",Sigma
R7306	T11301	T11302	compound	United,States
R7307	T11302	T11294	npadvmod	States,Sigma
R7308	T11303	T11294	punct	),Sigma
R7309	T11304	T11272	punct	.,changed
R731	T1469	T1470	punct	–,30
R7310	T11306	T11307	amod	Total,RNA
R7311	T11307	T11309	nsubjpass	RNA,prepared
R7312	T11308	T11307	amod	cellular,RNA
R7313	T11310	T11309	auxpass	was,prepared
R7314	T11311	T11312	mark	as,described
R7315	T11312	T11309	advcl	described,prepared
R7316	T11313	T11312	advmod	above,described
R7317	T11314	T11309	punct	", ",prepared
R7318	T11315	T11309	cc	and,prepared
R7319	T11316	T11317	nmod	osteocalcin,expression
R732	T1470	T1468	prep	30,25
R7320	T11317	T11326	nsubjpass	expression,quantified
R7321	T11318	T11316	punct	", ",osteocalcin
R7322	T11319	T11320	compound	β,actin
R7323	T11320	T11316	conj	actin,osteocalcin
R7324	T11321	T11320	punct	-,actin
R7325	T11322	T11320	punct	", ",actin
R7326	T11323	T11320	cc	and,actin
R7327	T11324	T11320	conj	GAPDH,actin
R7328	T11325	T11317	compound	gene,expression
R7329	T11326	T11309	conj	quantified,prepared
R733	T1471	T1468	punct	],25
R7330	T11327	T11317	prep	at,expression
R7331	T11328	T11329	amod	indicated,times
R7332	T11329	T11327	pobj	times,at
R7333	T11330	T11329	prep	after,times
R7334	T11331	T11332	nmod	BMP,treatment
R7335	T11332	T11330	pobj	treatment,after
R7336	T11333	T11331	punct	-,BMP
R7337	T11334	T11331	nummod	2,BMP
R7338	T11335	T11326	auxpass	was,quantified
R7339	T11336	T11326	prep	by,quantified
R734	T1472	T1444	punct	", ",remains
R7340	T11337	T11338	compound	RT,PCR
R7341	T11338	T11336	pobj	PCR,by
R7342	T11339	T11338	punct	-,PCR
R7343	T11340	T11326	punct	.,quantified
R735	T1473	T1474	det	the,role
R736	T1474	T1444	nsubj	role,remains
R737	T1475	T1474	prep	of,role
R738	T1476	T1477	compound	RNA,proteins
R739	T1477	T1475	pobj	proteins,of
R74	T314	T313	punct	−,Sam68
R740	T1478	T1477	compound	binding,proteins
R741	T1479	T1474	prep	in,role
R742	T1480	T1481	det	this,process
R743	T1481	T1479	pobj	process,in
R744	T1482	T1444	acomp	unknown,remains
R745	T1483	T1444	punct	.,remains
R746	T1485	T1486	compound	RNA,proteins
R747	T1486	T1488	nsubj	proteins,are
R748	T1487	T1486	compound	binding,proteins
R749	T1489	T1486	prep	of,proteins
R75	T315	T313	punct	/,Sam68
R750	T1490	T1491	det	the,type
R751	T1491	T1489	pobj	type,of
R752	T1492	T1491	compound	KH,type
R753	T1493	T1494	amod	known,regulators
R754	T1494	T1488	attr	regulators,are
R755	T1495	T1494	prep	of,regulators
R756	T1496	T1497	amod	cellular,differentiation
R757	T1497	T1495	pobj	differentiation,of
R758	T1498	T1488	punct	.,are
R759	T1500	T1501	prep	For,known
R76	T316	T313	punct	−,Sam68
R760	T1502	T1500	pobj	example,For
R761	T1503	T1501	punct	", ",known
R762	T1504	T1505	compound	expression,defects
R763	T1505	T1501	nsubjpass	defects,known
R764	T1506	T1505	prep	in,defects
R765	T1507	T1508	det	the,proteins
R766	T1508	T1506	pobj	proteins,in
R767	T1509	T1510	compound	KH,domain
R768	T1510	T1508	compound	domain,proteins
R769	T1511	T1508	appos	NOVA,proteins
R77	T318	T319	poss	their,mass
R770	T1512	T1511	cc	and,NOVA
R771	T1513	T1511	conj	FMRP,NOVA
R772	T1514	T1501	auxpass	are,known
R773	T1515	T1516	aux	to,cause
R774	T1516	T1501	xcomp	cause,known
R775	T1517	T1518	amod	paraneoplastic,disorders
R776	T1518	T1516	dobj	disorders,cause
R777	T1519	T1518	amod	neurologic,disorders
R778	T1520	T1521	punct	[,31
R779	T1521	T1518	parataxis	31,disorders
R78	T319	T317	dobj	mass,preserved
R780	T1522	T1521	punct	],31
R781	T1523	T1518	cc	and,disorders
R782	T1524	T1525	det	the,syndrome
R783	T1525	T1518	conj	syndrome,disorders
R784	T1526	T1527	amod	fragile,X
R785	T1527	T1525	compound	X,syndrome
R786	T1528	T1516	punct	", ",cause
R787	T1529	T1516	advmod	respectively,cause
R788	T1530	T1516	punct	", ",cause
R789	T1531	T1516	prep	in,cause
R79	T320	T319	compound	bone,mass
R790	T1532	T1531	pobj	humans,in
R791	T1533	T1534	punct	[,32
R792	T1534	T1516	parataxis	32,cause
R793	T1535	T1534	punct	],32
R794	T1536	T1501	punct	.,known
R795	T1538	T1539	det	The,phenotype
R796	T1539	T1540	nsubj	phenotype,suggests
R797	T1541	T1539	prep	of,phenotype
R798	T1542	T1543	det	the,mice
R799	T1543	T1541	pobj	mice,of
R8	T242	T241	compound	Sam68,Protein
R80	T321	T317	punct	", ",preserved
R800	T1544	T1543	nmod	quaking,mice
R801	T1545	T1543	amod	viable,mice
R802	T1546	T1547	det	a,role
R803	T1547	T1540	dobj	role,suggests
R804	T1548	T1547	prep	for,role
R805	T1549	T1550	det	the,protein
R806	T1550	T1548	pobj	protein,for
R807	T1551	T1550	compound	QUAKING,protein
R808	T1552	T1553	compound	RNA,binding
R809	T1553	T1550	compound	binding,protein
R81	T322	T317	prep	in,preserved
R810	T1554	T1547	prep	in,role
R811	T1555	T1554	pobj	oligodendrocytes,in
R812	T1556	T1555	cc	and,oligodendrocytes
R813	T1557	T1555	conj	myelination,oligodendrocytes
R814	T1558	T1559	punct	[,33
R815	T1559	T1540	parataxis	33,suggests
R816	T1560	T1559	punct	],33
R817	T1561	T1540	punct	.,suggests
R818	T1563	T1564	advmod	Indeed,led
R819	T1565	T1564	punct	", ",led
R82	T323	T324	amod	sharp,contrast
R820	T1566	T1567	det	the,expression
R821	T1567	T1564	nsubj	expression,led
R822	T1568	T1567	amod	ectopic,expression
R823	T1569	T1567	prep	of,expression
R824	T1570	T1571	det	the,isoforms
R825	T1571	T1569	pobj	isoforms,of
R826	T1572	T1573	nmod	QKI,7
R827	T1573	T1571	nummod	7,isoforms
R828	T1574	T1573	punct	-,7
R829	T1575	T1573	nummod	6,7
R83	T324	T322	pobj	contrast,in
R830	T1576	T1573	punct	/,7
R831	T1577	T1578	advmod	in,vivo
R832	T1578	T1564	advmod	vivo,led
R833	T1579	T1564	prep	to,led
R834	T1580	T1581	det	the,formation
R835	T1581	T1579	pobj	formation,to
R836	T1582	T1581	prep	of,formation
R837	T1583	T1584	amod	glial,cells
R838	T1584	T1582	pobj	cells,of
R839	T1585	T1586	advmod	rather,than
R84	T325	T324	prep	with,contrast
R840	T1586	T1584	cc	than,cells
R841	T1587	T1584	conj	neurons,cells
R842	T1588	T1581	prep	from,formation
R843	T1589	T1590	amod	neural,progenitor
R844	T1590	T1588	pobj	progenitor,from
R845	T1591	T1564	punct	", ",led
R846	T1592	T1564	advcl	demonstrating,led
R847	T1593	T1594	poss	its,role
R848	T1594	T1592	dobj	role,demonstrating
R849	T1595	T1594	prep	in,role
R85	T326	T327	nummod	12,month
R850	T1596	T1597	compound	cell,determination
R851	T1597	T1595	pobj	determination,in
R852	T1598	T1597	compound	fate,determination
R853	T1599	T1600	punct	[,34
R854	T1600	T1564	parataxis	34,led
R855	T1601	T1600	punct	],34
R856	T1602	T1564	punct	.,led
R857	T1604	T1605	det	The,loss
R858	T1605	T1606	nsubj	loss,prevents
R859	T1607	T1605	prep	of,loss
R86	T327	T329	npadvmod	month,old
R860	T1608	T1609	nmod	GLD,protein
R861	T1609	T1607	pobj	protein,of
R862	T1610	T1608	punct	-,GLD
R863	T1611	T1608	nummod	1,GLD
R864	T1612	T1605	prep	in,loss
R865	T1613	T1614	compound	Caenorhabditis,elegans
R866	T1614	T1612	pobj	elegans,in
R867	T1615	T1616	det	the,appearance
R868	T1616	T1606	dobj	appearance,prevents
R869	T1617	T1616	prep	of,appearance
R87	T328	T327	punct	-,month
R870	T1618	T1619	compound	stem,cells
R871	T1619	T1617	pobj	cells,of
R872	T1620	T1621	punct	[,35
R873	T1621	T1606	parataxis	35,prevents
R874	T1622	T1621	punct	],35
R875	T1623	T1606	punct	", ",prevents
R876	T1624	T1606	cc	and,prevents
R877	T1625	T1626	det	the,absence
R878	T1626	T1627	nsubj	absence,prevents
R879	T1627	T1606	conj	prevents,prevents
R88	T329	T331	amod	old,littermates
R880	T1628	T1626	prep	of,absence
R881	T1629	T1630	det	the,protein
R882	T1630	T1628	pobj	protein,of
R883	T1631	T1630	compound	KH,protein
R884	T1632	T1630	compound	domain,protein
R885	T1633	T1630	appos	HOW,protein
R886	T1634	T1626	prep	in,absence
R887	T1635	T1634	pobj	Drosophila,in
R888	T1636	T1637	compound	muscle,differentiation
R889	T1637	T1627	dobj	differentiation,prevents
R89	T330	T329	punct	-,old
R890	T1638	T1627	cc	and,prevents
R891	T1639	T1627	conj	results,prevents
R892	T1640	T1639	prep	in,results
R893	T1641	T1640	pobj	flies,in
R894	T1642	T1641	prep	with,flies
R895	T1643	T1644	amod	held,wings
R896	T1644	T1642	pobj	wings,with
R897	T1645	T1643	punct	-,held
R898	T1646	T1643	prt	out,held
R899	T1647	T1644	punct	-,wings
R9	T243	T244	compound	RNA,Binding
R90	T331	T325	pobj	littermates,with
R900	T1648	T1649	punct	[,37
R901	T1649	T1639	parataxis	37,results
R902	T1650	T1649	nummod	36,37
R903	T1651	T1649	punct	",",37
R904	T1652	T1649	punct	],37
R905	T1653	T1627	punct	.,prevents
R906	T1655	T1656	det	The,substrate
R907	T1656	T1658	nsubj	substrate,is
R908	T1657	T1656	compound	Src,substrate
R909	T1658	T1668	ccomp	is,remained
R91	T332	T333	amod	wild,type
R910	T1659	T1656	acl	associated,substrate
R911	T1660	T1659	prep	in,associated
R912	T1661	T1660	pobj	mitosis,in
R913	T1662	T1661	prep	of,mitosis
R914	T1663	T1664	nummod	68,kDa
R915	T1664	T1662	pobj	kDa,of
R916	T1665	T1666	punct	(,Sam68
R917	T1666	T1659	parataxis	Sam68,associated
R918	T1667	T1666	punct	),Sam68
R919	T1669	T1658	advmod	also,is
R92	T333	T331	compound	type,littermates
R920	T1670	T1671	det	a,member
R921	T1671	T1658	attr	member,is
R922	T1672	T1671	prep	of,member
R923	T1673	T1674	det	the,family
R924	T1674	T1672	pobj	family,of
R925	T1675	T1674	prep	of,family
R926	T1676	T1677	compound	KH,proteins
R927	T1677	T1675	pobj	proteins,of
R928	T1678	T1677	compound	domain,proteins
R929	T1679	T1680	compound	RNA,binding
R93	T334	T333	punct	-,type
R930	T1680	T1677	compound	binding,proteins
R931	T1681	T1682	punct	[,38
R932	T1682	T1658	parataxis	38,is
R933	T1683	T1682	punct	],38
R934	T1684	T1668	punct	;,remained
R935	T1685	T1668	advmod	however,remained
R936	T1686	T1668	punct	", ",remained
R937	T1687	T1688	poss	its,role
R938	T1688	T1668	nsubj	role,remained
R939	T1689	T1688	amod	physiologic,role
R94	T335	T336	prep	in,decreased
R940	T1690	T1668	aux	has,remained
R941	T1691	T1668	acomp	undefined,remained
R942	T1692	T1668	punct	.,remained
R943	T1721	T1719	prep	42,39
R944	T1694	T1695	nsubjpass	Sam68,identified
R945	T1722	T1719	punct	],39
R946	T1723	T1715	cc	and,of
R947	T1724	T1715	conj	of,of
R948	T1725	T1726	det	the,kinase
R949	T1696	T1695	auxpass	was,identified
R95	T336	T331	relcl	decreased,littermates
R950	T1726	T1724	pobj	kinase,of
R951	T1727	T1728	compound	breast,tumor
R952	T1728	T1726	compound	tumor,kinase
R953	T1729	T1730	punct	[,43
R954	T1697	T1695	prep	as,identified
R955	T1730	T1726	parataxis	43,kinase
R956	T1731	T1730	punct	],43
R957	T1698	T1699	det	an,protein
R958	T1732	T1695	punct	.,identified
R959	T1734	T1735	nsubjpass	Sam68,shown
R96	T337	T335	pobj	which,in
R960	T1736	T1735	aux	has,shown
R961	T1737	T1735	auxpass	been,shown
R962	T1699	T1697	pobj	protein,as
R963	T1738	T1739	aux	to,facilitate
R964	T1700	T1699	nmod	SH3,protein
R965	T1739	T1735	xcomp	facilitate,shown
R966	T1701	T1700	cc	and,SH3
R967	T1702	T1700	conj	SH2,SH3
R968	T1740	T1741	det	the,export
R969	T1703	T1699	nmod	domain,protein
R97	T338	T339	compound	bone,mass
R970	T1741	T1739	dobj	export,facilitate
R971	T1704	T1699	amod	interacting,protein
R972	T1742	T1741	prep	of,export
R973	T1743	T1744	amod	unspliced,RNA
R974	T1744	T1742	pobj	RNA,of
R975	T1705	T1695	prep	for,identified
R976	T1745	T1744	compound	HIV,RNA
R977	T1746	T1747	punct	[,44
R978	T1747	T1739	parataxis	44,facilitate
R979	T1706	T1707	compound	Src,kinases
R98	T339	T336	nsubjpass	mass,decreased
R980	T1748	T1747	punct	],44
R981	T1749	T1739	cc	and,facilitate
R982	T1750	T1751	aux	to,regulate
R983	T1707	T1705	pobj	kinases,for
R984	T1751	T1739	conj	regulate,facilitate
R985	T1752	T1753	compound	pre-mRNA,processing
R986	T1708	T1707	compound	family,kinases
R987	T1753	T1751	dobj	processing,regulate
R988	T1754	T1755	punct	[,45
R989	T1755	T1751	parataxis	45,regulate
R99	T340	T336	auxpass	was,decreased
R990	T1709	T1695	cc	and,identified
R991	T1756	T1755	punct	],45
R992	T1757	T1735	punct	.,shown
R993	T1759	T1760	prep	In,report
R994	T1710	T1695	conj	is,identified
R995	T1761	T1762	det	the,paper
R996	T1711	T1710	advmod	also,is
R997	T1762	T1759	pobj	paper,In
R998	T1763	T1762	amod	present,paper
R999	T1764	T1760	punct	", ",report

PMC:1315279 JSONTXT 8 Projects

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PMC:1315279 JSON TXT 8 Projects