PMC:1310620 / 5792-6422 5 Projects
Annnotations
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1609 | 0-5 | JJ | denotes | Total |
| T1611 | 6-9 | NN | denotes | RNA |
| T1610 | 10-17 | NNS | denotes | samples |
| T1613 | 18-27 | VBN | denotes | extracted |
| T1614 | 28-32 | IN | denotes | from |
| T1615 | 33-37 | NN | denotes | NBEC |
| T1616 | 38-42 | VBD | denotes | were |
| T1617 | 43-50 | RB | denotes | reverse |
| T1612 | 51-62 | VBN | denotes | transcribed |
| T1618 | 63-68 | VBG | denotes | using |
| T1619 | 69-70 | NN | denotes | M |
| T1621 | 70-71 | HYPH | denotes | - |
| T1620 | 71-74 | NN | denotes | MLV |
| T1623 | 75-82 | JJ | denotes | reverse |
| T1622 | 83-96 | NN | denotes | transcriptase |
| T1624 | 97-100 | CC | denotes | and |
| T1625 | 101-106 | NN | denotes | oligo |
| T1626 | 107-109 | NN | denotes | dT |
| T1627 | 110-117 | NNS | denotes | primers |
| T1628 | 118-120 | IN | denotes | as |
| T1630 | 121-131 | RB | denotes | previously |
| T1629 | 132-141 | VBN | denotes | described |
| T1631 | 142-143 | -LRB- | denotes | [ |
| T1633 | 143-144 | CD | denotes | 9 |
| T1634 | 144-145 | , | denotes | , |
| T1632 | 145-147 | CD | denotes | 11 |
| T1635 | 147-148 | -RRB- | denotes | ] |
| T1636 | 148-149 | . | denotes | . |
| T1637 | 149-241 | sentence | denotes | Standardized RT (StaRT)-PCR was used for transcript abundance measurement in these studies. |
| T1638 | 150-162 | VBN | denotes | Standardized |
| T1639 | 163-165 | NN | denotes | RT |
| T1641 | 166-167 | -LRB- | denotes | ( |
| T1642 | 167-172 | NN | denotes | StaRT |
| T1643 | 172-173 | -RRB- | denotes | ) |
| T1644 | 173-174 | HYPH | denotes | - |
| T1640 | 174-177 | NN | denotes | PCR |
| T1646 | 178-181 | VBD | denotes | was |
| T1645 | 182-186 | VBN | denotes | used |
| T1647 | 187-190 | IN | denotes | for |
| T1648 | 191-201 | NN | denotes | transcript |
| T1649 | 202-211 | NN | denotes | abundance |
| T1650 | 212-223 | NN | denotes | measurement |
| T1651 | 224-226 | IN | denotes | in |
| T1652 | 227-232 | DT | denotes | these |
| T1653 | 233-240 | NNS | denotes | studies |
| T1654 | 240-241 | . | denotes | . |
| T1655 | 241-385 | sentence | denotes | With StaRT-PCR, an internal standard for each gene within a standardized mixture of internal standards (SMIS) is included in each PCR reaction. |
| T1656 | 242-246 | IN | denotes | With |
| T1658 | 247-252 | NN | denotes | StaRT |
| T1660 | 252-253 | HYPH | denotes | - |
| T1659 | 253-256 | NN | denotes | PCR |
| T1661 | 256-258 | , | denotes | , |
| T1662 | 258-260 | DT | denotes | an |
| T1664 | 261-269 | JJ | denotes | internal |
| T1663 | 270-278 | NN | denotes | standard |
| T1665 | 279-282 | IN | denotes | for |
| T1666 | 283-287 | DT | denotes | each |
| T1667 | 288-292 | NN | denotes | gene |
| T1668 | 293-299 | IN | denotes | within |
| T1669 | 300-301 | DT | denotes | a |
| T1671 | 302-314 | VBN | denotes | standardized |
| T1670 | 315-322 | NN | denotes | mixture |
| T1672 | 323-325 | IN | denotes | of |
| T1673 | 326-334 | JJ | denotes | internal |
| T1674 | 335-344 | NNS | denotes | standards |
| T1675 | 345-346 | -LRB- | denotes | ( |
| T1676 | 346-350 | NN | denotes | SMIS |
| T1677 | 350-351 | -RRB- | denotes | ) |
| T1678 | 352-354 | VBZ | denotes | is |
| T1657 | 355-363 | VBN | denotes | included |
| T1679 | 364-366 | IN | denotes | in |
| T1680 | 367-371 | DT | denotes | each |
| T1682 | 372-375 | NN | denotes | PCR |
| T1681 | 376-384 | NN | denotes | reaction |
| T1683 | 384-385 | . | denotes | . |
| T1684 | 385-630 | sentence | denotes | After amplification, products were electrophoresed on an Agilent 2100 Bioanalyzer using DNA Chips with DNA 1000 Kit reagents for visualization according to the manufacturer's protocol (Agilent Technologies Deutschland GmbH, Waldbronn, Germany). |
| T1685 | 386-391 | IN | denotes | After |
| T1687 | 392-405 | NN | denotes | amplification |
| T1688 | 405-407 | , | denotes | , |
| T1689 | 407-415 | NNS | denotes | products |
| T1690 | 416-420 | VBD | denotes | were |
| T1686 | 421-436 | VBN | denotes | electrophoresed |
| T1691 | 437-439 | IN | denotes | on |
| T1692 | 440-442 | DT | denotes | an |
| T1694 | 443-450 | NNP | denotes | Agilent |
| T1695 | 451-455 | CD | denotes | 2100 |
| T1693 | 456-467 | NNP | denotes | Bioanalyzer |
| T1696 | 468-473 | VBG | denotes | using |
| T1697 | 474-477 | NN | denotes | DNA |
| T1698 | 478-483 | NNS | denotes | Chips |
| T1699 | 484-488 | IN | denotes | with |
| T1700 | 489-492 | NN | denotes | DNA |
| T1702 | 493-497 | CD | denotes | 1000 |
| T1701 | 498-501 | NN | denotes | Kit |
| T1703 | 502-510 | NNS | denotes | reagents |
| T1704 | 511-514 | IN | denotes | for |
| T1705 | 515-528 | NN | denotes | visualization |
| T1706 | 529-538 | VBG | denotes | according |
| T1707 | 539-541 | IN | denotes | to |
| T1708 | 542-545 | DT | denotes | the |
| T1709 | 546-558 | NN | denotes | manufacturer |
| T1711 | 558-560 | POS | denotes | 's |
| T1710 | 561-569 | NN | denotes | protocol |
| T1712 | 570-571 | -LRB- | denotes | ( |
| T1714 | 571-578 | NNP | denotes | Agilent |
| T1715 | 579-591 | NNP | denotes | Technologies |
| T1716 | 592-603 | NNP | denotes | Deutschland |
| T1713 | 604-608 | NNP | denotes | GmbH |
| T1717 | 608-610 | , | denotes | , |
| T1718 | 610-619 | NNP | denotes | Waldbronn |
| T1719 | 619-621 | , | denotes | , |
| T1720 | 621-628 | NNP | denotes | Germany |
| T1721 | 628-629 | -RRB- | denotes | ) |
| T1722 | 629-630 | . | denotes | . |
| R1000 | T1670 | T1668 | pobj | mixture,within |
| R1001 | T1671 | T1670 | amod | standardized,mixture |
| R1002 | T1672 | T1670 | prep | of,mixture |
| R1003 | T1673 | T1674 | amod | internal,standards |
| R1004 | T1674 | T1672 | pobj | standards,of |
| R1005 | T1675 | T1670 | punct | (,mixture |
| R1006 | T1676 | T1670 | appos | SMIS,mixture |
| R1007 | T1677 | T1657 | punct | ),included |
| R1008 | T1678 | T1657 | auxpass | is,included |
| R1009 | T1679 | T1657 | prep | in,included |
| R1010 | T1680 | T1681 | det | each,reaction |
| R1011 | T1681 | T1679 | pobj | reaction,in |
| R1012 | T1682 | T1681 | compound | PCR,reaction |
| R1013 | T1683 | T1657 | punct | .,included |
| R1014 | T1685 | T1686 | prep | After,electrophoresed |
| R1015 | T1687 | T1685 | pobj | amplification,After |
| R1016 | T1688 | T1686 | punct | ", ",electrophoresed |
| R1017 | T1689 | T1686 | nsubjpass | products,electrophoresed |
| R1018 | T1690 | T1686 | auxpass | were,electrophoresed |
| R1019 | T1691 | T1686 | prep | on,electrophoresed |
| R1020 | T1692 | T1693 | det | an,Bioanalyzer |
| R1021 | T1693 | T1691 | pobj | Bioanalyzer,on |
| R1022 | T1694 | T1693 | nmod | Agilent,Bioanalyzer |
| R1023 | T1695 | T1694 | nummod | 2100,Agilent |
| R1024 | T1696 | T1686 | advcl | using,electrophoresed |
| R1025 | T1697 | T1698 | compound | DNA,Chips |
| R1026 | T1698 | T1696 | dobj | Chips,using |
| R1027 | T1699 | T1696 | prep | with,using |
| R1028 | T1700 | T1701 | nmod | DNA,Kit |
| R1029 | T1701 | T1703 | compound | Kit,reagents |
| R1030 | T1702 | T1701 | nummod | 1000,Kit |
| R1031 | T1703 | T1699 | pobj | reagents,with |
| R1032 | T1704 | T1696 | prep | for,using |
| R1033 | T1705 | T1704 | pobj | visualization,for |
| R1034 | T1706 | T1696 | prep | according,using |
| R1035 | T1707 | T1706 | prep | to,according |
| R1036 | T1708 | T1709 | det | the,manufacturer |
| R1037 | T1709 | T1710 | poss | manufacturer,protocol |
| R1038 | T1710 | T1707 | pobj | protocol,to |
| R1039 | T1711 | T1709 | case | 's,manufacturer |
| R1040 | T1712 | T1713 | punct | (,GmbH |
| R1041 | T1713 | T1696 | parataxis | GmbH,using |
| R1042 | T1714 | T1715 | compound | Agilent,Technologies |
| R1043 | T1715 | T1713 | compound | Technologies,GmbH |
| R1044 | T1716 | T1713 | compound | Deutschland,GmbH |
| R1045 | T1717 | T1713 | punct | ", ",GmbH |
| R1046 | T1718 | T1713 | npadvmod | Waldbronn,GmbH |
| R1047 | T1719 | T1713 | punct | ", ",GmbH |
| R1048 | T1720 | T1713 | npadvmod | Germany,GmbH |
| R1049 | T1721 | T1713 | punct | ),GmbH |
| R1050 | T1722 | T1686 | punct | .,electrophoresed |
| R944 | T1609 | T1610 | amod | Total,samples |
| R945 | T1610 | T1612 | nsubjpass | samples,transcribed |
| R946 | T1611 | T1610 | compound | RNA,samples |
| R947 | T1613 | T1610 | acl | extracted,samples |
| R948 | T1614 | T1613 | prep | from,extracted |
| R949 | T1615 | T1614 | pobj | NBEC,from |
| R950 | T1616 | T1612 | auxpass | were,transcribed |
| R951 | T1617 | T1612 | advmod | reverse,transcribed |
| R952 | T1618 | T1612 | advcl | using,transcribed |
| R953 | T1619 | T1620 | nmod | M,MLV |
| R954 | T1620 | T1622 | nmod | MLV,transcriptase |
| R955 | T1621 | T1620 | punct | -,MLV |
| R956 | T1622 | T1618 | dobj | transcriptase,using |
| R957 | T1623 | T1622 | amod | reverse,transcriptase |
| R958 | T1624 | T1622 | cc | and,transcriptase |
| R959 | T1625 | T1626 | compound | oligo,dT |
| R960 | T1626 | T1627 | compound | dT,primers |
| R961 | T1627 | T1622 | conj | primers,transcriptase |
| R962 | T1628 | T1629 | mark | as,described |
| R963 | T1629 | T1612 | advcl | described,transcribed |
| R964 | T1630 | T1629 | advmod | previously,described |
| R965 | T1631 | T1632 | punct | [,11 |
| R966 | T1632 | T1612 | parataxis | 11,transcribed |
| R967 | T1633 | T1632 | nummod | 9,11 |
| R968 | T1634 | T1632 | punct | ",",11 |
| R969 | T1635 | T1632 | punct | ],11 |
| R970 | T1636 | T1612 | punct | .,transcribed |
| R971 | T1638 | T1639 | amod | Standardized,RT |
| R972 | T1639 | T1640 | nmod | RT,PCR |
| R973 | T1640 | T1645 | nsubjpass | PCR,used |
| R974 | T1641 | T1639 | punct | (,RT |
| R975 | T1642 | T1639 | appos | StaRT,RT |
| R976 | T1643 | T1640 | punct | ),PCR |
| R977 | T1644 | T1640 | punct | -,PCR |
| R978 | T1646 | T1645 | auxpass | was,used |
| R979 | T1647 | T1645 | prep | for,used |
| R980 | T1648 | T1649 | compound | transcript,abundance |
| R981 | T1649 | T1650 | compound | abundance,measurement |
| R982 | T1650 | T1647 | pobj | measurement,for |
| R983 | T1651 | T1645 | prep | in,used |
| R984 | T1652 | T1653 | det | these,studies |
| R985 | T1653 | T1651 | pobj | studies,in |
| R986 | T1654 | T1645 | punct | .,used |
| R987 | T1656 | T1657 | prep | With,included |
| R988 | T1658 | T1659 | compound | StaRT,PCR |
| R989 | T1659 | T1656 | pobj | PCR,With |
| R990 | T1660 | T1659 | punct | -,PCR |
| R991 | T1661 | T1657 | punct | ", ",included |
| R992 | T1662 | T1663 | det | an,standard |
| R993 | T1663 | T1657 | nsubjpass | standard,included |
| R994 | T1664 | T1663 | amod | internal,standard |
| R995 | T1665 | T1663 | prep | for,standard |
| R996 | T1666 | T1667 | det | each,gene |
| R997 | T1667 | T1665 | pobj | gene,for |
| R998 | T1668 | T1663 | prep | within,standard |
| R999 | T1669 | T1670 | det | a,mixture |