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PMC:1283364 / 9550-9831 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T2641 0-3 DT denotes the
T2642 4-12 NN denotes presence
T2643 13-15 IN denotes of
T2644 16-20 DT denotes each
T2645 21-30 NN denotes transgene
T2646 31-33 IN denotes by
T2647 34-37 NN denotes PCR
T2648 38-51 NN denotes amplification
T2649 52-54 IN denotes of
T2650 55-62 JJ denotes genomic
T2651 63-66 NN denotes DNA
T2652 67-76 VBN denotes extracted
T2653 77-81 IN denotes from
T2654 82-83 DT denotes a
T2656 84-85 CD denotes 5
T2658 85-86 HYPH denotes -
T2657 86-88 NN denotes mm
T2659 89-93 NN denotes tail
T2655 94-100 NN denotes biopsy
T2660 100-101 . denotes .
T2661 101-240 sentence denotes Tails were heated to 95 °C for 45 min in 250 μl of 50 mM NaOH, vortexed, then neutralized with an equal volume of 0.5 M Tris-HCl (pH 5.5).
T2662 102-107 NNS denotes Tails
T2664 108-112 VBD denotes were
T2663 113-119 VBN denotes heated
T2665 120-122 IN denotes to
T2666 123-125 CD denotes 95
T2667 126-128 NN denotes °C
T2668 129-132 IN denotes for
T2669 133-135 CD denotes 45
T2670 136-139 NN denotes min
T2671 140-142 IN denotes in
T2672 143-146 CD denotes 250
T2673 147-149 NN denotes μl
T2674 150-152 IN denotes of
T2675 153-155 CD denotes 50
T2676 156-158 NN denotes mM
T2677 159-163 NN denotes NaOH
T2678 163-165 , denotes ,
T2679 165-173 VBN denotes vortexed
T2680 173-175 , denotes ,
T2681 175-179 RB denotes then
T2682 180-191 VBN denotes neutralized
T2683 192-196 IN denotes with
T2684 197-199 DT denotes an
T2686 200-205 JJ denotes equal
T2685 206-212 NN denotes volume
T2687 213-215 IN denotes of
T2688 216-219 CD denotes 0.5
T2689 220-221 NN denotes M
T2691 222-226 NN denotes Tris
T2692 226-227 HYPH denotes -
T2690 227-230 NN denotes HCl
T2693 231-232 -LRB- denotes (
T2694 232-234 NN denotes pH
T2695 235-238 CD denotes 5.5
T2696 238-239 -RRB- denotes )
T2697 239-240 . denotes .
T2699 241-247 NN denotes Debris
T2701 248-251 VBD denotes was
T2700 252-262 VBN denotes sedimented
T2702 263-265 IN denotes by
T2703 266-280 NN denotes centrifugation
R1721 T2641 T2642 det the,presence
R1723 T2643 T2642 prep of,presence
R1724 T2644 T2645 det each,transgene
R1725 T2645 T2643 pobj transgene,of
R1727 T2647 T2648 compound PCR,amplification
R1728 T2648 T2646 pobj amplification,by
R1729 T2649 T2648 prep of,amplification
R1730 T2650 T2651 amod genomic,DNA
R1731 T2651 T2649 pobj DNA,of
R1732 T2652 T2651 acl extracted,DNA
R1733 T2653 T2652 prep from,extracted
R1734 T2654 T2655 det a,biopsy
R1735 T2655 T2653 pobj biopsy,from
R1736 T2656 T2657 nummod 5,mm
R1737 T2657 T2655 compound mm,biopsy
R1738 T2658 T2657 punct -,mm
R1739 T2659 T2655 compound tail,biopsy
R1741 T2662 T2663 nsubjpass Tails,heated
R1742 T2664 T2663 auxpass were,heated
R1743 T2665 T2663 prep to,heated
R1744 T2666 T2667 nummod 95,°C
R1745 T2667 T2665 pobj °C,to
R1746 T2668 T2663 prep for,heated
R1747 T2669 T2670 nummod 45,min
R1748 T2670 T2668 pobj min,for
R1749 T2671 T2663 prep in,heated
R1750 T2672 T2673 nummod 250,μl
R1751 T2673 T2671 pobj μl,in
R1752 T2674 T2673 prep of,μl
R1753 T2675 T2676 nummod 50,mM
R1754 T2676 T2677 compound mM,NaOH
R1755 T2677 T2674 pobj NaOH,of
R1756 T2678 T2663 punct ", ",heated
R1757 T2679 T2663 dep vortexed,heated
R1758 T2680 T2663 punct ", ",heated
R1759 T2681 T2682 advmod then,neutralized
R1760 T2682 T2663 dep neutralized,heated
R1761 T2683 T2682 prep with,neutralized
R1762 T2684 T2685 det an,volume
R1763 T2685 T2683 pobj volume,with
R1764 T2686 T2685 amod equal,volume
R1765 T2687 T2685 prep of,volume
R1766 T2688 T2689 nummod 0.5,M
R1767 T2689 T2690 compound M,HCl
R1768 T2690 T2687 pobj HCl,of
R1769 T2691 T2690 compound Tris,HCl
R1770 T2692 T2690 punct -,HCl
R1771 T2693 T2694 punct (,pH
R1772 T2694 T2690 parataxis pH,HCl
R1773 T2695 T2694 nummod 5.5,pH
R1774 T2696 T2694 punct ),pH
R1775 T2697 T2663 punct .,heated
R1776 T2699 T2700 nsubjpass Debris,sedimented
R1777 T2701 T2700 auxpass was,sedimented
R1778 T2702 T2700 prep by,sedimented
R1779 T2703 T2702 pobj centrifugation,by