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PMC:1283364 / 5493-7974 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T1535 0-9 NN denotes Transgene
T1536 10-22 NN denotes Construction
T1537 22-422 sentence denotes We created a tetracycline-responsive chimeric mouse/human APP695Swedish/Indiana (swe/ind) vector by replacing the promoter region of the moPrP.XhoI vector (also known as pPrPpE1/E2,3sal [15]) with the tetracycline-responsive promoter of pTetSplice (Life Technologies, Rockville, Maryland, United States), and then ligating mouse APP with a humanized Aβ domain (mo/huAPP695) cDNA into the new vector.
T1538 23-25 PRP denotes We
T1539 26-33 VBD denotes created
T1540 34-35 DT denotes a
T1542 36-48 NN denotes tetracycline
T1544 48-49 HYPH denotes -
T1543 49-59 JJ denotes responsive
T1545 60-68 JJ denotes chimeric
T1546 69-74 NN denotes mouse
T1547 74-75 HYPH denotes /
T1548 75-80 JJ denotes human
T1549 81-94 NN denotes APP695Swedish
T1551 94-95 HYPH denotes /
T1550 95-102 NNP denotes Indiana
T1552 103-104 -LRB- denotes (
T1553 104-107 NN denotes swe
T1555 107-108 HYPH denotes /
T1554 108-111 NN denotes ind
T1556 111-112 -RRB- denotes )
T1541 113-119 NN denotes vector
T1557 120-122 IN denotes by
T1558 123-132 VBG denotes replacing
T1559 133-136 DT denotes the
T1561 137-145 NN denotes promoter
T1560 146-152 NN denotes region
T1562 153-155 IN denotes of
T1563 156-159 DT denotes the
T1565 160-170 NN denotes moPrP.XhoI
T1564 171-177 NN denotes vector
T1566 178-179 -LRB- denotes (
T1568 179-183 RB denotes also
T1567 184-189 VBN denotes known
T1569 190-192 IN denotes as
T1570 193-200 NN denotes pPrPpE1
T1572 200-201 HYPH denotes /
T1573 201-203 NN denotes E2
T1574 203-204 , denotes ,
T1571 204-208 NN denotes 3sal
T1575 209-210 -LRB- denotes [
T1576 210-212 CD denotes 15
T1577 212-213 -RRB- denotes ]
T1578 213-214 -RRB- denotes )
T1579 215-219 IN denotes with
T1580 220-223 DT denotes the
T1582 224-236 NN denotes tetracycline
T1584 236-237 HYPH denotes -
T1583 237-247 JJ denotes responsive
T1581 248-256 NN denotes promoter
T1585 257-259 IN denotes of
T1586 260-270 NN denotes pTetSplice
T1587 271-272 -LRB- denotes (
T1589 272-276 NNP denotes Life
T1588 277-289 NNP denotes Technologies
T1590 289-291 , denotes ,
T1591 291-300 NNP denotes Rockville
T1592 300-302 , denotes ,
T1593 302-310 NNP denotes Maryland
T1594 310-312 , denotes ,
T1595 312-318 NNP denotes United
T1596 319-325 NNP denotes States
T1597 325-326 -RRB- denotes )
T1598 326-328 , denotes ,
T1599 328-331 CC denotes and
T1600 332-336 RB denotes then
T1601 337-345 VBG denotes ligating
T1602 346-351 NN denotes mouse
T1603 352-355 NN denotes APP
T1604 356-360 IN denotes with
T1605 361-362 DT denotes a
T1607 363-372 JJ denotes humanized
T1608 373-375 NN denotes Aβ
T1606 376-382 NN denotes domain
T1610 383-384 -LRB- denotes (
T1611 384-386 NN denotes mo
T1613 386-387 HYPH denotes /
T1612 387-395 NN denotes huAPP695
T1614 395-396 -RRB- denotes )
T1609 397-401 NN denotes cDNA
T1615 402-406 IN denotes into
T1616 407-410 DT denotes the
T1618 411-414 JJ denotes new
T1617 415-421 NN denotes vector
T1619 421-422 . denotes .
T1620 422-792 sentence denotes We began by cloning the tetracycline-responsive promoter (bp 6–481) from pTetSplice by PCR using primers that added external BamHI and NotI sites to the 5′ end and a BamHI site to the 3′ end, while destroying XhoI and BamHI sites within the promoter (forward: GCC GGA TCC GCG GCC GCC GTC GAG TTT ACC ACT CCC TAT C; reverse: GCC GGA TCC ACT CTA GAA GAT CCC CGG GTA CCG).
T1621 423-425 PRP denotes We
T1622 426-431 VBD denotes began
T1623 432-434 IN denotes by
T1624 435-442 VBG denotes cloning
T1625 443-446 DT denotes the
T1627 447-459 NN denotes tetracycline
T1629 459-460 HYPH denotes -
T1628 460-470 JJ denotes responsive
T1626 471-479 NN denotes promoter
T1630 480-481 -LRB- denotes (
T1632 481-483 NN denotes bp
T1631 484-485 CD denotes 6
T1633 485-486 SYM denotes –
T1634 486-489 CD denotes 481
T1635 489-490 -RRB- denotes )
T1636 491-495 IN denotes from
T1637 496-506 NN denotes pTetSplice
T1638 507-509 IN denotes by
T1639 510-513 NN denotes PCR
T1640 514-519 VBG denotes using
T1641 520-527 NNS denotes primers
T1642 528-532 WDT denotes that
T1643 533-538 VBD denotes added
T1644 539-547 JJ denotes external
T1646 548-553 NN denotes BamHI
T1647 554-557 CC denotes and
T1648 558-562 NN denotes NotI
T1645 563-568 NNS denotes sites
T1649 569-571 IN denotes to
T1650 572-575 DT denotes the
T1652 576-577 CD denotes 5
T1653 577-578 SYM denotes ′
T1651 579-582 NN denotes end
T1654 583-586 CC denotes and
T1655 587-588 DT denotes a
T1657 589-594 NN denotes BamHI
T1656 595-599 NN denotes site
T1658 600-602 IN denotes to
T1659 603-606 DT denotes the
T1661 607-608 CD denotes 3
T1662 608-609 SYM denotes ′
T1660 610-613 NN denotes end
T1663 613-615 , denotes ,
T1664 615-620 IN denotes while
T1665 621-631 VBG denotes destroying
T1666 632-636 NN denotes XhoI
T1668 637-640 CC denotes and
T1669 641-646 NN denotes BamHI
T1667 647-652 NNS denotes sites
T1670 653-659 IN denotes within
T1671 660-663 DT denotes the
T1672 664-672 NN denotes promoter
T1673 673-674 -LRB- denotes (
T1675 674-681 NN denotes forward
T1677 681-683 : denotes :
T1678 683-686 NN denotes GCC
T1679 687-690 NN denotes GGA
T1680 691-694 NN denotes TCC
T1681 695-698 NN denotes GCG
T1682 699-702 NN denotes GCC
T1683 703-706 NN denotes GCC
T1684 707-710 NN denotes GTC
T1685 711-714 NN denotes GAG
T1686 715-718 NN denotes TTT
T1687 719-722 NN denotes ACC
T1688 723-726 NN denotes ACT
T1689 727-730 NN denotes CCC
T1690 731-734 NN denotes TAT
T1676 735-736 NN denotes C
T1691 736-737 : denotes ;
T1692 738-745 NN denotes reverse
T1693 745-747 : denotes :
T1694 747-750 NN denotes GCC
T1695 751-754 NN denotes GGA
T1696 755-758 NN denotes TCC
T1697 759-762 NN denotes ACT
T1698 763-766 NN denotes CTA
T1699 767-770 NN denotes GAA
T1700 771-774 NN denotes GAT
T1701 775-778 NN denotes CCC
T1702 779-782 NN denotes CGG
T1703 783-786 NN denotes GTA
T1674 787-790 NN denotes CCG
T1704 790-791 -RRB- denotes )
T1705 791-792 . denotes .
T1706 792-1058 sentence denotes We then isolated the moPrP.XhoI intron by amplification with primers that added an external BamHI site to the 5′ end of exon 1 and ran through the Asp718 site of exon 2 (forward: GCC GGA TCC GAT CAG CAG ACC GAT TCT GG; reverse: GCC GGT ACC ACT AGG AAG GCA GAA TGC).
T1707 793-795 PRP denotes We
T1709 796-800 RB denotes then
T1708 801-809 VBD denotes isolated
T1710 810-813 DT denotes the
T1712 814-824 NN denotes moPrP.XhoI
T1711 825-831 NN denotes intron
T1713 832-834 IN denotes by
T1714 835-848 NN denotes amplification
T1715 849-853 IN denotes with
T1716 854-861 NNS denotes primers
T1717 862-866 WDT denotes that
T1718 867-872 VBD denotes added
T1719 873-875 DT denotes an
T1721 876-884 JJ denotes external
T1722 885-890 NN denotes BamHI
T1720 891-895 NN denotes site
T1723 896-898 IN denotes to
T1724 899-902 DT denotes the
T1726 903-904 CD denotes 5
T1727 904-905 SYM denotes ′
T1725 906-909 NN denotes end
T1728 910-912 IN denotes of
T1729 913-917 NN denotes exon
T1730 918-919 CD denotes 1
T1731 920-923 CC denotes and
T1732 924-927 VBD denotes ran
T1733 928-935 IN denotes through
T1734 936-939 DT denotes the
T1736 940-946 NN denotes Asp718
T1735 947-951 NN denotes site
T1737 952-954 IN denotes of
T1738 955-959 NN denotes exon
T1739 960-961 CD denotes 2
T1740 962-963 -LRB- denotes (
T1742 963-970 NN denotes forward
T1744 970-972 : denotes :
T1745 972-975 NN denotes GCC
T1746 976-979 NN denotes GGA
T1747 980-983 NN denotes TCC
T1748 984-987 NN denotes GAT
T1749 988-991 NN denotes CAG
T1750 992-995 NN denotes CAG
T1751 996-999 NN denotes ACC
T1752 1000-1003 NN denotes GAT
T1753 1004-1007 NN denotes TCT
T1743 1008-1010 NN denotes GG
T1754 1010-1011 : denotes ;
T1755 1012-1019 NN denotes reverse
T1756 1019-1021 : denotes :
T1757 1021-1024 NN denotes GCC
T1758 1025-1028 NN denotes GGT
T1759 1029-1032 NN denotes ACC
T1760 1033-1036 NN denotes ACT
T1761 1037-1040 NN denotes AGG
T1762 1041-1044 NN denotes AAG
T1763 1045-1048 NN denotes GCA
T1764 1049-1052 NN denotes GAA
T1741 1053-1056 NN denotes TGC
T1765 1056-1057 -RRB- denotes )
T1766 1057-1058 . denotes .
T1767 1058-1409 sentence denotes This 2-kb intron fragment was cloned into a TA cloning vector (Invitrogen, Carlsbad, California, United States), then excised by Asp718 digestion and ligated to the 6.8-kb Asp718 fragment of moPrP.XhoI containing exon 2, exon 3, the 3′ UTR, and pBluescript to generate an intermediate vector with all three exons and a central intron but no promoter.
T1768 1059-1063 DT denotes This
T1770 1064-1065 CD denotes 2
T1772 1065-1066 HYPH denotes -
T1771 1066-1068 NN denotes kb
T1773 1069-1075 NN denotes intron
T1769 1076-1084 NN denotes fragment
T1775 1085-1088 VBD denotes was
T1774 1089-1095 VBN denotes cloned
T1776 1096-1100 IN denotes into
T1777 1101-1102 DT denotes a
T1779 1103-1105 NN denotes TA
T1780 1106-1113 NN denotes cloning
T1778 1114-1120 NN denotes vector
T1781 1121-1122 -LRB- denotes (
T1782 1122-1132 NNP denotes Invitrogen
T1783 1132-1134 , denotes ,
T1784 1134-1142 NNP denotes Carlsbad
T1785 1142-1144 , denotes ,
T1786 1144-1154 NNP denotes California
T1787 1154-1156 , denotes ,
T1788 1156-1162 NNP denotes United
T1789 1163-1169 NNP denotes States
T1790 1169-1170 -RRB- denotes )
T1791 1170-1172 , denotes ,
T1792 1172-1176 RB denotes then
T1793 1177-1184 VBN denotes excised
T1794 1185-1187 IN denotes by
T1795 1188-1194 NN denotes Asp718
T1796 1195-1204 NN denotes digestion
T1797 1205-1208 CC denotes and
T1798 1209-1216 VBN denotes ligated
T1799 1217-1219 IN denotes to
T1800 1220-1223 DT denotes the
T1802 1224-1227 CD denotes 6.8
T1804 1227-1228 HYPH denotes -
T1803 1228-1230 NN denotes kb
T1805 1231-1237 NN denotes Asp718
T1801 1238-1246 NN denotes fragment
T1806 1247-1249 IN denotes of
T1807 1250-1260 NN denotes moPrP.XhoI
T1808 1261-1271 VBG denotes containing
T1809 1272-1276 NN denotes exon
T1810 1277-1278 CD denotes 2
T1811 1278-1280 , denotes ,
T1812 1280-1284 NN denotes exon
T1813 1285-1286 CD denotes 3
T1814 1286-1288 , denotes ,
T1815 1288-1291 DT denotes the
T1817 1292-1293 CD denotes 3
T1818 1293-1294 SYM denotes ′
T1816 1295-1298 NN denotes UTR
T1819 1298-1300 , denotes ,
T1820 1300-1303 CC denotes and
T1821 1304-1315 NN denotes pBluescript
T1822 1316-1318 TO denotes to
T1823 1319-1327 VB denotes generate
T1824 1328-1330 DT denotes an
T1826 1331-1343 JJ denotes intermediate
T1825 1344-1350 NN denotes vector
T1827 1351-1355 IN denotes with
T1828 1356-1359 DT denotes all
T1830 1360-1365 CD denotes three
T1829 1366-1371 NNS denotes exons
T1831 1372-1375 CC denotes and
T1832 1376-1377 DT denotes a
T1834 1378-1385 JJ denotes central
T1833 1386-1392 NN denotes intron
T1835 1393-1396 CC denotes but
T1836 1397-1399 DT denotes no
T1837 1400-1408 NN denotes promoter
T1838 1408-1409 . denotes .
T1839 1409-1564 sentence denotes This vector was then opened at the BamHI site introduced by the intron cloning primer, and ligated to the 0.5-kb BamHI-cut tetracycline promoter fragment.
T1840 1410-1414 DT denotes This
T1841 1415-1421 NN denotes vector
T1843 1422-1425 VBD denotes was
T1844 1426-1430 RB denotes then
T1842 1431-1437 VBN denotes opened
T1845 1438-1440 IN denotes at
T1846 1441-1444 DT denotes the
T1848 1445-1450 NN denotes BamHI
T1847 1451-1455 NN denotes site
T1849 1456-1466 VBN denotes introduced
T1850 1467-1469 IN denotes by
T1851 1470-1473 DT denotes the
T1853 1474-1480 NN denotes intron
T1854 1481-1488 NN denotes cloning
T1852 1489-1495 NN denotes primer
T1855 1495-1497 , denotes ,
T1856 1497-1500 CC denotes and
T1857 1501-1508 VBN denotes ligated
T1858 1509-1511 IN denotes to
T1859 1512-1515 DT denotes the
T1861 1516-1519 CD denotes 0.5
T1863 1519-1520 HYPH denotes -
T1862 1520-1522 NN denotes kb
T1864 1523-1528 NN denotes BamHI
T1866 1528-1529 HYPH denotes -
T1865 1529-1532 NN denotes cut
T1867 1533-1545 NN denotes tetracycline
T1868 1546-1554 NN denotes promoter
T1860 1555-1563 NN denotes fragment
T1869 1563-1564 . denotes .
T1870 1564-1780 sentence denotes This ligation generated a 9.3-kb vector encoding the tetracycline promoter from pTetSplice with two exons, one intron, and the original 3′ UTR of the moPrP.XhoI vector, all carried in the pBluescript cloning vector.
T1871 1565-1569 DT denotes This
T1872 1570-1578 NN denotes ligation
T1873 1579-1588 VBD denotes generated
T1874 1589-1590 DT denotes a
T1876 1591-1594 CD denotes 9.3
T1878 1594-1595 HYPH denotes -
T1877 1595-1597 NN denotes kb
T1875 1598-1604 NN denotes vector
T1879 1605-1613 VBG denotes encoding
T1880 1614-1617 DT denotes the
T1882 1618-1630 NN denotes tetracycline
T1881 1631-1639 NN denotes promoter
T1883 1640-1644 IN denotes from
T1884 1645-1655 NN denotes pTetSplice
T1885 1656-1660 IN denotes with
T1886 1661-1664 CD denotes two
T1887 1665-1670 NNS denotes exons
T1888 1670-1672 , denotes ,
T1889 1672-1675 CD denotes one
T1890 1676-1682 NN denotes intron
T1891 1682-1684 , denotes ,
T1892 1684-1687 CC denotes and
T1893 1688-1691 DT denotes the
T1895 1692-1700 JJ denotes original
T1896 1701-1702 CD denotes 3
T1897 1702-1703 SYM denotes ′
T1894 1704-1707 NN denotes UTR
T1898 1708-1710 IN denotes of
T1899 1711-1714 DT denotes the
T1901 1715-1725 NN denotes moPrP.XhoI
T1900 1726-1732 NN denotes vector
T1902 1732-1734 , denotes ,
T1903 1734-1737 RB denotes all
T1904 1738-1745 VBN denotes carried
T1905 1746-1748 IN denotes in
T1906 1749-1752 DT denotes the
T1908 1753-1764 NNP denotes pBluescript
T1909 1765-1772 NN denotes cloning
T1907 1773-1779 NN denotes vector
T1910 1779-1780 . denotes .
T1911 1780-2230 sentence denotes We incorporated the Swedish (KM570/571NL) and Indiana (V617F) mutations into the mo/huAPP695 cDNA (in BS-KS) by PCR using a four-primer strategy: first, two partially overlapping products were generated in separate reactions using primers that encode the desired mutations (Swedish forward: GGA GAT CTC TGA AGT GAA TCT GGA TGC AGA ATT CCG/Indiana reverse: GGG TGA TGA AAA TCA CGG TTG C; Indiana forward: CAA CCG TGA TTT TCA TCA CCC TGG/M13 reverse).
T1912 1781-1783 PRP denotes We
T1913 1784-1796 VBD denotes incorporated
T1915 1797-1800 DT denotes the
T1917 1801-1808 NNP denotes Swedish
T1918 1809-1810 -LRB- denotes (
T1920 1810-1815 NN denotes KM570
T1921 1815-1816 HYPH denotes /
T1919 1816-1821 NN denotes 571NL
T1922 1821-1822 -RRB- denotes )
T1923 1823-1826 CC denotes and
T1924 1827-1834 NNP denotes Indiana
T1925 1835-1836 -LRB- denotes (
T1926 1836-1841 NN denotes V617F
T1927 1841-1842 -RRB- denotes )
T1916 1843-1852 NNS denotes mutations
T1928 1853-1857 IN denotes into
T1929 1858-1861 DT denotes the
T1931 1862-1864 NN denotes mo
T1933 1864-1865 HYPH denotes /
T1932 1865-1873 NN denotes huAPP695
T1930 1874-1878 NN denotes cDNA
T1934 1879-1880 -LRB- denotes (
T1935 1880-1882 IN denotes in
T1936 1883-1885 NN denotes BS
T1938 1885-1886 HYPH denotes -
T1937 1886-1888 NN denotes KS
T1939 1888-1889 -RRB- denotes )
T1940 1890-1892 IN denotes by
T1941 1893-1896 NN denotes PCR
T1942 1897-1902 VBG denotes using
T1943 1903-1904 DT denotes a
T1945 1905-1909 CD denotes four
T1947 1909-1910 HYPH denotes -
T1946 1910-1916 NN denotes primer
T1944 1917-1925 NN denotes strategy
T1948 1925-1927 : denotes :
T1949 1927-1932 RB denotes first
T1950 1932-1934 , denotes ,
T1951 1934-1937 CD denotes two
T1953 1938-1947 RB denotes partially
T1954 1948-1959 VBG denotes overlapping
T1952 1960-1968 NNS denotes products
T1955 1969-1973 VBD denotes were
T1914 1974-1983 VBN denotes generated
T1956 1984-1986 IN denotes in
T1957 1987-1995 JJ denotes separate
T1958 1996-2005 NNS denotes reactions
T1959 2006-2011 VBG denotes using
T1960 2012-2019 NNS denotes primers
T1961 2020-2024 WDT denotes that
T1962 2025-2031 VBP denotes encode
T1963 2032-2035 DT denotes the
T1965 2036-2043 VBN denotes desired
T1964 2044-2053 NNS denotes mutations
T1966 2054-2055 -LRB- denotes (
T1968 2055-2062 NNP denotes Swedish
T1969 2063-2070 NN denotes forward
T1971 2070-2072 : denotes :
T1972 2072-2075 NN denotes GGA
T1973 2076-2079 NN denotes GAT
T1974 2080-2083 NN denotes CTC
T1975 2084-2087 NN denotes TGA
T1976 2088-2091 NN denotes AGT
T1977 2092-2095 NN denotes GAA
T1978 2096-2099 NN denotes TCT
T1979 2100-2103 NNP denotes GGA
T1980 2104-2107 NNP denotes TGC
T1981 2108-2111 NNP denotes AGA
T1982 2112-2115 NNP denotes ATT
T1970 2116-2119 NN denotes CCG
T1984 2119-2120 HYPH denotes /
T1985 2120-2127 NNP denotes Indiana
T1986 2128-2135 NN denotes reverse
T1987 2135-2137 : denotes :
T1988 2137-2140 NN denotes GGG
T1989 2141-2144 NN denotes TGA
T1990 2145-2148 NN denotes TGA
T1991 2149-2152 NN denotes AAA
T1992 2153-2156 NN denotes TCA
T1993 2157-2160 NN denotes CGG
T1994 2161-2164 NN denotes TTG
T1983 2165-2166 NN denotes C
T1995 2166-2167 : denotes ;
T1996 2168-2175 NNP denotes Indiana
T1997 2176-2183 NN denotes forward
T1998 2183-2185 : denotes :
T1999 2185-2188 NN denotes CAA
T2000 2189-2192 NN denotes CCG
T2001 2193-2196 NN denotes TGA
T2002 2197-2200 NN denotes TTT
T2003 2201-2204 NN denotes TCA
T2004 2205-2208 NN denotes TCA
T2005 2209-2212 NN denotes CCC
T2006 2213-2216 NN denotes TGG
T2007 2216-2217 HYPH denotes /
T1967 2217-2220 NN denotes M13
T2008 2221-2228 RB denotes reverse
T2009 2228-2229 -RRB- denotes )
T2010 2229-2230 . denotes .
T2011 2230-2354 sentence denotes The two PCR products were ligated, digested with BglII and ApaI and cloned back into the original mo/huAPP695-BS-KS vector.
T2012 2231-2234 DT denotes The
T2014 2235-2238 CD denotes two
T2015 2239-2242 NN denotes PCR
T2013 2243-2251 NNS denotes products
T2017 2252-2256 VBD denotes were
T2016 2257-2264 VBN denotes ligated
T2018 2264-2266 , denotes ,
T2019 2266-2274 VBN denotes digested
T2020 2275-2279 IN denotes with
T2021 2280-2285 NN denotes BglII
T2022 2286-2289 CC denotes and
T2023 2290-2294 NN denotes ApaI
T2024 2295-2298 CC denotes and
T2025 2299-2305 VBN denotes cloned
T2026 2306-2310 RB denotes back
T2027 2311-2315 IN denotes into
T2028 2316-2319 DT denotes the
T2030 2320-2328 JJ denotes original
T2031 2329-2331 NN denotes mo
T2033 2331-2332 HYPH denotes /
T2034 2332-2340 NN denotes huAPP695
T2035 2340-2341 HYPH denotes -
T2036 2341-2343 NN denotes BS
T2037 2343-2344 HYPH denotes -
T2032 2344-2346 NN denotes KS
T2029 2347-2353 NN denotes vector
T2038 2353-2354 . denotes .
T2039 2354-2481 sentence denotes Finally, the new APP695swe/ind was subcloned into the XhoI site of the moPrP-tetP vector from above to complete the construct.
T2040 2355-2362 RB denotes Finally
T2042 2362-2364 , denotes ,
T2043 2364-2367 DT denotes the
T2045 2368-2371 JJ denotes new
T2046 2372-2381 NN denotes APP695swe
T2047 2381-2382 HYPH denotes /
T2044 2382-2385 NN denotes ind
T2048 2386-2389 VBD denotes was
T2041 2390-2399 VBN denotes subcloned
T2049 2400-2404 IN denotes into
T2050 2405-2408 DT denotes the
T2052 2409-2413 NN denotes XhoI
T2051 2414-2418 NN denotes site
T2053 2419-2421 IN denotes of
T2054 2422-2425 DT denotes the
T2056 2426-2431 NN denotes moPrP
T2058 2431-2432 HYPH denotes -
T2057 2432-2436 NN denotes tetP
T2055 2437-2443 NN denotes vector
T2059 2444-2448 IN denotes from
T2060 2449-2454 RB denotes above
T2061 2455-2457 TO denotes to
T2062 2458-2466 VB denotes complete
T2063 2467-2470 DT denotes the
T2064 2471-2480 NN denotes construct
T2065 2480-2481 . denotes .
R1000 T1625 T1626 det the,promoter
R1001 T1626 T1624 dobj promoter,cloning
R1002 T1627 T1628 npadvmod tetracycline,responsive
R1003 T1628 T1626 amod responsive,promoter
R1004 T1629 T1628 punct -,responsive
R1005 T1630 T1631 punct (,6
R1006 T1631 T1626 parataxis 6,promoter
R1007 T1632 T1631 nmod bp,6
R1008 T1633 T1634 punct –,481
R1009 T1634 T1631 prep 481,6
R1010 T1635 T1631 punct ),6
R1011 T1636 T1624 prep from,cloning
R1012 T1637 T1636 pobj pTetSplice,from
R1013 T1638 T1637 prep by,pTetSplice
R1014 T1639 T1638 pobj PCR,by
R1015 T1640 T1639 acl using,PCR
R1016 T1641 T1640 dobj primers,using
R1017 T1642 T1643 dep that,added
R1018 T1643 T1641 relcl added,primers
R1019 T1644 T1645 amod external,sites
R1020 T1645 T1643 dobj sites,added
R1021 T1646 T1645 nmod BamHI,sites
R1022 T1647 T1646 cc and,BamHI
R1023 T1648 T1646 conj NotI,BamHI
R1024 T1649 T1643 prep to,added
R1025 T1650 T1651 det the,end
R1026 T1651 T1649 pobj end,to
R1027 T1652 T1651 nummod 5,end
R1028 T1653 T1652 punct ′,5
R1029 T1654 T1643 cc and,added
R1030 T1655 T1656 det a,site
R1031 T1656 T1643 conj site,added
R1032 T1657 T1656 compound BamHI,site
R1033 T1658 T1656 prep to,site
R1034 T1659 T1660 det the,end
R1035 T1660 T1658 pobj end,to
R1036 T1661 T1660 nummod 3,end
R1037 T1662 T1661 punct ′,3
R1038 T1663 T1622 punct ", ",began
R1039 T1664 T1665 mark while,destroying
R1040 T1665 T1622 advcl destroying,began
R1041 T1666 T1667 nmod XhoI,sites
R1042 T1667 T1665 dobj sites,destroying
R1043 T1668 T1666 cc and,XhoI
R1044 T1669 T1666 conj BamHI,XhoI
R1045 T1670 T1667 prep within,sites
R1046 T1671 T1672 det the,promoter
R1047 T1672 T1670 pobj promoter,within
R1048 T1673 T1674 punct (,CCG
R1049 T1674 T1665 parataxis CCG,destroying
R1050 T1675 T1676 dep forward,C
R1051 T1676 T1674 dep C,CCG
R1052 T1677 T1676 punct : ,C
R1053 T1678 T1676 compound GCC,C
R1054 T1679 T1676 compound GGA,C
R1055 T1680 T1676 compound TCC,C
R1056 T1681 T1676 compound GCG,C
R1057 T1682 T1676 compound GCC,C
R1058 T1683 T1676 compound GCC,C
R1059 T1684 T1676 compound GTC,C
R1060 T1685 T1676 compound GAG,C
R1061 T1686 T1676 compound TTT,C
R1062 T1687 T1676 compound ACC,C
R1063 T1688 T1676 compound ACT,C
R1064 T1689 T1676 compound CCC,C
R1065 T1690 T1676 compound TAT,C
R1066 T1691 T1674 punct ;,CCG
R1067 T1692 T1674 dep reverse,CCG
R1068 T1693 T1674 punct : ,CCG
R1069 T1694 T1674 compound GCC,CCG
R1070 T1695 T1674 compound GGA,CCG
R1071 T1696 T1674 compound TCC,CCG
R1072 T1697 T1674 compound ACT,CCG
R1073 T1698 T1674 compound CTA,CCG
R1074 T1699 T1674 compound GAA,CCG
R1075 T1700 T1674 compound GAT,CCG
R1076 T1701 T1674 compound CCC,CCG
R1077 T1702 T1674 compound CGG,CCG
R1078 T1703 T1674 compound GTA,CCG
R1079 T1704 T1674 punct ),CCG
R1080 T1705 T1622 punct .,began
R1081 T1707 T1708 nsubj We,isolated
R1082 T1709 T1708 advmod then,isolated
R1083 T1710 T1711 det the,intron
R1084 T1711 T1708 dobj intron,isolated
R1085 T1712 T1711 compound moPrP.XhoI,intron
R1086 T1713 T1708 prep by,isolated
R1087 T1714 T1713 pobj amplification,by
R1088 T1715 T1714 prep with,amplification
R1089 T1716 T1715 pobj primers,with
R1090 T1717 T1718 dep that,added
R1091 T1718 T1716 relcl added,primers
R1092 T1719 T1720 det an,site
R1093 T1720 T1718 dobj site,added
R1094 T1721 T1720 amod external,site
R1095 T1722 T1720 compound BamHI,site
R1096 T1723 T1718 prep to,added
R1097 T1724 T1725 det the,end
R1098 T1725 T1723 pobj end,to
R1099 T1726 T1725 nummod 5,end
R1100 T1727 T1726 punct ′,5
R1101 T1728 T1725 prep of,end
R1102 T1729 T1728 pobj exon,of
R1103 T1730 T1729 nummod 1,exon
R1104 T1731 T1718 cc and,added
R1105 T1732 T1718 conj ran,added
R1106 T1733 T1732 prep through,ran
R1107 T1734 T1735 det the,site
R1108 T1735 T1733 pobj site,through
R1109 T1736 T1735 compound Asp718,site
R1110 T1737 T1735 prep of,site
R1111 T1738 T1737 pobj exon,of
R1112 T1739 T1738 nummod 2,exon
R1113 T1740 T1741 punct (,TGC
R1114 T1741 T1735 parataxis TGC,site
R1115 T1742 T1743 dep forward,GG
R1116 T1743 T1741 dep GG,TGC
R1117 T1744 T1743 punct : ,GG
R1118 T1745 T1743 compound GCC,GG
R1119 T1746 T1743 compound GGA,GG
R1120 T1747 T1743 compound TCC,GG
R1121 T1748 T1743 compound GAT,GG
R1122 T1749 T1743 compound CAG,GG
R1123 T1750 T1743 compound CAG,GG
R1124 T1751 T1743 compound ACC,GG
R1125 T1752 T1743 compound GAT,GG
R1126 T1753 T1743 compound TCT,GG
R1127 T1754 T1741 punct ;,TGC
R1128 T1755 T1741 dep reverse,TGC
R1129 T1756 T1741 punct : ,TGC
R1130 T1757 T1741 compound GCC,TGC
R1131 T1758 T1741 compound GGT,TGC
R1132 T1759 T1741 compound ACC,TGC
R1133 T1760 T1741 compound ACT,TGC
R1134 T1761 T1741 compound AGG,TGC
R1135 T1762 T1741 compound AAG,TGC
R1136 T1763 T1741 compound GCA,TGC
R1137 T1764 T1741 compound GAA,TGC
R1138 T1765 T1741 punct ),TGC
R1139 T1766 T1708 punct .,isolated
R1140 T1768 T1769 det This,fragment
R1141 T1769 T1774 nsubj fragment,cloned
R1142 T1770 T1771 nummod 2,kb
R1143 T1771 T1769 compound kb,fragment
R1144 T1772 T1771 punct -,kb
R1145 T1773 T1769 compound intron,fragment
R1146 T1775 T1774 aux was,cloned
R1147 T1776 T1774 prep into,cloned
R1148 T1777 T1778 det a,vector
R1149 T1778 T1776 pobj vector,into
R1150 T1779 T1780 compound TA,cloning
R1151 T1780 T1778 compound cloning,vector
R1152 T1781 T1782 punct (,Invitrogen
R1153 T1782 T1778 parataxis Invitrogen,vector
R1154 T1783 T1782 punct ", ",Invitrogen
R1155 T1784 T1782 npadvmod Carlsbad,Invitrogen
R1156 T1785 T1782 punct ", ",Invitrogen
R1157 T1786 T1782 npadvmod California,Invitrogen
R1158 T1787 T1782 punct ", ",Invitrogen
R1159 T1788 T1789 compound United,States
R1160 T1789 T1782 npadvmod States,Invitrogen
R1161 T1790 T1782 punct ),Invitrogen
R1162 T1791 T1774 punct ", ",cloned
R1163 T1792 T1793 advmod then,excised
R1164 T1793 T1774 conj excised,cloned
R1165 T1794 T1793 prep by,excised
R1166 T1795 T1796 compound Asp718,digestion
R1167 T1796 T1794 pobj digestion,by
R1168 T1797 T1793 cc and,excised
R1169 T1798 T1793 conj ligated,excised
R1170 T1799 T1798 prep to,ligated
R1171 T1800 T1801 det the,fragment
R1172 T1801 T1799 pobj fragment,to
R1173 T1802 T1803 nummod 6.8,kb
R1174 T1803 T1801 compound kb,fragment
R1175 T1804 T1803 punct -,kb
R1176 T1805 T1801 compound Asp718,fragment
R1177 T1806 T1801 prep of,fragment
R1178 T1807 T1806 pobj moPrP.XhoI,of
R1179 T1808 T1801 acl containing,fragment
R1180 T1809 T1808 dobj exon,containing
R1181 T1810 T1809 nummod 2,exon
R1182 T1811 T1809 punct ", ",exon
R1183 T1812 T1809 conj exon,exon
R1184 T1813 T1812 nummod 3,exon
R1185 T1814 T1812 punct ", ",exon
R1186 T1815 T1816 det the,UTR
R1187 T1816 T1812 conj UTR,exon
R1188 T1817 T1816 nummod 3,UTR
R1189 T1818 T1817 punct ′,3
R1190 T1819 T1816 punct ", ",UTR
R1191 T1820 T1816 cc and,UTR
R1192 T1821 T1816 conj pBluescript,UTR
R1193 T1822 T1823 aux to,generate
R1194 T1823 T1774 advcl generate,cloned
R1195 T1824 T1825 det an,vector
R1196 T1825 T1823 dobj vector,generate
R1197 T1826 T1825 amod intermediate,vector
R1198 T1827 T1823 prep with,generate
R1199 T1828 T1829 det all,exons
R1200 T1829 T1827 pobj exons,with
R1201 T1830 T1829 nummod three,exons
R1202 T1831 T1829 cc and,exons
R1203 T1832 T1833 det a,intron
R1204 T1833 T1829 conj intron,exons
R1205 T1834 T1833 amod central,intron
R1206 T1835 T1829 cc but,exons
R1207 T1836 T1837 det no,promoter
R1208 T1837 T1829 conj promoter,exons
R1209 T1838 T1774 punct .,cloned
R1210 T1840 T1841 det This,vector
R1211 T1841 T1842 nsubjpass vector,opened
R1212 T1843 T1842 auxpass was,opened
R1213 T1844 T1842 advmod then,opened
R1214 T1845 T1842 prep at,opened
R1215 T1846 T1847 det the,site
R1216 T1847 T1845 pobj site,at
R1217 T1848 T1847 compound BamHI,site
R1218 T1849 T1847 acl introduced,site
R1219 T1850 T1849 agent by,introduced
R1220 T1851 T1852 det the,primer
R1221 T1852 T1850 pobj primer,by
R1222 T1853 T1852 compound intron,primer
R1223 T1854 T1852 compound cloning,primer
R1224 T1855 T1842 punct ", ",opened
R1225 T1856 T1842 cc and,opened
R1226 T1857 T1842 conj ligated,opened
R1227 T1858 T1857 prep to,ligated
R1228 T1859 T1860 det the,fragment
R1229 T1860 T1858 pobj fragment,to
R1230 T1861 T1862 nummod 0.5,kb
R1231 T1862 T1860 compound kb,fragment
R1232 T1863 T1862 punct -,kb
R1233 T1864 T1865 compound BamHI,cut
R1234 T1865 T1860 compound cut,fragment
R1235 T1866 T1865 punct -,cut
R1236 T1867 T1868 compound tetracycline,promoter
R1237 T1868 T1860 compound promoter,fragment
R1238 T1869 T1842 punct .,opened
R1239 T1871 T1872 det This,ligation
R1240 T1872 T1873 nsubj ligation,generated
R1241 T1874 T1875 det a,vector
R1242 T1875 T1873 dobj vector,generated
R1243 T1876 T1877 nummod 9.3,kb
R1244 T1877 T1875 compound kb,vector
R1245 T1878 T1877 punct -,kb
R1246 T1879 T1875 acl encoding,vector
R1247 T1880 T1881 det the,promoter
R1248 T1881 T1879 dobj promoter,encoding
R1249 T1882 T1881 compound tetracycline,promoter
R1250 T1883 T1881 prep from,promoter
R1251 T1884 T1883 pobj pTetSplice,from
R1252 T1885 T1879 prep with,encoding
R1253 T1886 T1887 nummod two,exons
R1254 T1887 T1885 pobj exons,with
R1255 T1888 T1887 punct ", ",exons
R1256 T1889 T1890 nummod one,intron
R1257 T1890 T1887 conj intron,exons
R1258 T1891 T1890 punct ", ",intron
R1259 T1892 T1890 cc and,intron
R1260 T1893 T1894 det the,UTR
R1261 T1894 T1890 conj UTR,intron
R1262 T1895 T1894 amod original,UTR
R1263 T1896 T1894 nummod 3,UTR
R1264 T1897 T1896 punct ′,3
R1265 T1898 T1894 prep of,UTR
R1266 T1899 T1900 det the,vector
R1267 T1900 T1898 pobj vector,of
R1268 T1901 T1900 compound moPrP.XhoI,vector
R1269 T1902 T1887 punct ", ",exons
R1270 T1903 T1904 advmod all,carried
R1271 T1904 T1887 acl carried,exons
R1272 T1905 T1904 prep in,carried
R1273 T1906 T1907 det the,vector
R1274 T1907 T1905 pobj vector,in
R1275 T1908 T1909 compound pBluescript,cloning
R1276 T1909 T1907 compound cloning,vector
R1277 T1910 T1873 punct .,generated
R1278 T1912 T1913 nsubj We,incorporated
R1279 T1913 T1914 ccomp incorporated,generated
R1280 T1915 T1916 det the,mutations
R1281 T1916 T1913 dobj mutations,incorporated
R1282 T1917 T1916 amod Swedish,mutations
R1283 T1918 T1919 punct (,571NL
R1284 T1919 T1917 parataxis 571NL,Swedish
R1285 T1920 T1919 compound KM570,571NL
R1286 T1921 T1919 punct /,571NL
R1287 T1922 T1919 punct ),571NL
R1288 T1923 T1917 cc and,Swedish
R1289 T1924 T1917 conj Indiana,Swedish
R1290 T1925 T1926 punct (,V617F
R1291 T1926 T1924 parataxis V617F,Indiana
R1292 T1927 T1926 punct ),V617F
R1293 T1928 T1913 prep into,incorporated
R1294 T1929 T1930 det the,cDNA
R1295 T1930 T1928 pobj cDNA,into
R1296 T1931 T1932 compound mo,huAPP695
R1297 T1932 T1930 compound huAPP695,cDNA
R1298 T1933 T1932 punct /,huAPP695
R1299 T1934 T1930 punct (,cDNA
R1300 T1935 T1930 prep in,cDNA
R1301 T1936 T1937 compound BS,KS
R1302 T1937 T1935 pobj KS,in
R1303 T1938 T1937 punct -,KS
R1304 T1939 T1913 punct ),incorporated
R1305 T1940 T1913 prep by,incorporated
R1306 T1941 T1940 pobj PCR,by
R1307 T1942 T1913 advcl using,incorporated
R1308 T1943 T1944 det a,strategy
R1309 T1944 T1942 dobj strategy,using
R1310 T1945 T1946 nummod four,primer
R1311 T1946 T1944 compound primer,strategy
R1312 T1947 T1946 punct -,primer
R1313 T1948 T1914 punct : ,generated
R1314 T1949 T1914 advmod first,generated
R1315 T1950 T1914 punct ", ",generated
R1316 T1951 T1952 nummod two,products
R1317 T1952 T1914 nsubjpass products,generated
R1318 T1953 T1954 advmod partially,overlapping
R1319 T1954 T1952 amod overlapping,products
R1320 T1955 T1914 auxpass were,generated
R1321 T1956 T1914 prep in,generated
R1322 T1957 T1958 amod separate,reactions
R1323 T1958 T1956 pobj reactions,in
R1324 T1959 T1914 advcl using,generated
R1325 T1960 T1959 dobj primers,using
R1326 T1961 T1962 dep that,encode
R1327 T1962 T1960 relcl encode,primers
R1328 T1963 T1964 det the,mutations
R1329 T1964 T1962 dobj mutations,encode
R1330 T1965 T1964 amod desired,mutations
R1331 T1966 T1967 punct (,M13
R1332 T1967 T1962 parataxis M13,encode
R1333 T1968 T1969 compound Swedish,forward
R1334 T1969 T1970 dep forward,CCG
R1335 T1970 T1983 dep CCG,C
R1336 T1971 T1970 punct : ,CCG
R1337 T1972 T1970 compound GGA,CCG
R1338 T1973 T1970 compound GAT,CCG
R1339 T1974 T1970 compound CTC,CCG
R1340 T1975 T1970 compound TGA,CCG
R1341 T1976 T1970 compound AGT,CCG
R1342 T1977 T1970 compound GAA,CCG
R1343 T1978 T1970 compound TCT,CCG
R1344 T1979 T1970 compound GGA,CCG
R1345 T1980 T1970 compound TGC,CCG
R1346 T1981 T1970 compound AGA,CCG
R1347 T1982 T1970 compound ATT,CCG
R1348 T1983 T1967 dep C,M13
R1349 T1984 T1983 punct /,C
R1350 T1985 T1986 compound Indiana,reverse
R1351 T1986 T1983 dep reverse,C
R1352 T1987 T1983 punct : ,C
R1353 T1988 T1983 compound GGG,C
R1354 T1989 T1983 compound TGA,C
R1355 T1990 T1983 compound TGA,C
R1356 T1991 T1983 compound AAA,C
R1357 T1992 T1983 compound TCA,C
R1358 T1993 T1983 compound CGG,C
R1359 T1994 T1983 compound TTG,C
R1360 T1995 T1967 punct ;,M13
R1361 T1996 T1997 compound Indiana,forward
R1362 T1997 T1967 dep forward,M13
R1363 T1998 T1967 punct : ,M13
R1364 T1999 T1967 compound CAA,M13
R1365 T2000 T1967 compound CCG,M13
R1366 T2001 T1967 compound TGA,M13
R1367 T2002 T1967 compound TTT,M13
R1368 T2003 T1967 compound TCA,M13
R1369 T2004 T1967 compound TCA,M13
R1370 T2005 T1967 compound CCC,M13
R1371 T2006 T1967 compound TGG,M13
R1372 T2007 T1967 punct /,M13
R1373 T2008 T1967 advmod reverse,M13
R1374 T2009 T1967 punct ),M13
R1375 T2010 T1914 punct .,generated
R1376 T2012 T2013 det The,products
R1377 T2013 T2016 nsubjpass products,ligated
R1378 T2014 T2013 nummod two,products
R1379 T2015 T2013 compound PCR,products
R1380 T2017 T2016 auxpass were,ligated
R1381 T2018 T2016 punct ", ",ligated
R1382 T2019 T2016 dep digested,ligated
R1383 T2020 T2016 prep with,ligated
R1384 T2021 T2020 pobj BglII,with
R1385 T2022 T2021 cc and,BglII
R1386 T2023 T2021 conj ApaI,BglII
R1387 T2024 T2016 cc and,ligated
R1388 T2025 T2016 conj cloned,ligated
R1389 T2026 T2025 advmod back,cloned
R1390 T2027 T2025 prep into,cloned
R1391 T2028 T2029 det the,vector
R1392 T2029 T2027 pobj vector,into
R1393 T2030 T2029 amod original,vector
R1394 T2031 T2032 compound mo,KS
R1395 T2032 T2029 compound KS,vector
R1396 T2033 T2032 punct /,KS
R1397 T2034 T2032 compound huAPP695,KS
R1398 T2035 T2032 punct -,KS
R1399 T2036 T2032 compound BS,KS
R1400 T2037 T2032 punct -,KS
R1401 T2038 T2016 punct .,ligated
R1402 T2040 T2041 advmod Finally,subcloned
R1403 T2042 T2041 punct ", ",subcloned
R1404 T2043 T2044 det the,ind
R1405 T2044 T2041 nsubjpass ind,subcloned
R1406 T2045 T2044 amod new,ind
R1407 T2046 T2044 compound APP695swe,ind
R1408 T2047 T2044 punct /,ind
R1409 T2048 T2041 auxpass was,subcloned
R1410 T2049 T2041 prep into,subcloned
R1411 T2050 T2051 det the,site
R1412 T2051 T2049 pobj site,into
R1413 T2052 T2051 compound XhoI,site
R1414 T2053 T2051 prep of,site
R1415 T2054 T2055 det the,vector
R1416 T2055 T2053 pobj vector,of
R1417 T2056 T2057 compound moPrP,tetP
R1418 T2057 T2055 compound tetP,vector
R1419 T2058 T2057 punct -,tetP
R1420 T2059 T2041 prep from,subcloned
R1421 T2060 T2059 pcomp above,from
R1422 T2061 T2062 aux to,complete
R1423 T2062 T2041 advcl complete,subcloned
R1424 T2063 T2064 det the,construct
R1425 T2064 T2062 dobj construct,complete
R1426 T2065 T2041 punct .,subcloned
R915 T1535 T1536 compound Transgene,Construction
R916 T1538 T1539 nsubj We,created
R917 T1540 T1541 det a,vector
R918 T1541 T1539 dobj vector,created
R919 T1542 T1543 npadvmod tetracycline,responsive
R920 T1543 T1541 amod responsive,vector
R921 T1544 T1543 punct -,responsive
R922 T1545 T1541 amod chimeric,vector
R923 T1546 T1541 nmod mouse,vector
R924 T1547 T1546 punct /,mouse
R925 T1548 T1546 amod human,mouse
R926 T1549 T1550 nmod APP695Swedish,Indiana
R927 T1550 T1541 nmod Indiana,vector
R928 T1551 T1550 punct /,Indiana
R929 T1552 T1550 punct (,Indiana
R930 T1553 T1554 compound swe,ind
R931 T1554 T1550 appos ind,Indiana
R932 T1555 T1554 punct /,ind
R933 T1556 T1541 punct ),vector
R934 T1557 T1539 prep by,created
R935 T1558 T1557 pcomp replacing,by
R936 T1559 T1560 det the,region
R937 T1560 T1558 dobj region,replacing
R938 T1561 T1560 compound promoter,region
R939 T1562 T1560 prep of,region
R940 T1563 T1564 det the,vector
R941 T1564 T1562 pobj vector,of
R942 T1565 T1564 compound moPrP.XhoI,vector
R943 T1566 T1567 punct (,known
R944 T1567 T1558 parataxis known,replacing
R945 T1568 T1567 advmod also,known
R946 T1569 T1567 prep as,known
R947 T1570 T1571 nmod pPrPpE1,3sal
R948 T1571 T1569 pobj 3sal,as
R949 T1572 T1571 punct /,3sal
R950 T1573 T1571 nmod E2,3sal
R951 T1574 T1571 punct ",",3sal
R952 T1575 T1576 punct [,15
R953 T1576 T1567 parataxis 15,known
R954 T1577 T1576 punct ],15
R955 T1578 T1567 punct ),known
R956 T1579 T1558 prep with,replacing
R957 T1580 T1581 det the,promoter
R958 T1581 T1579 pobj promoter,with
R959 T1582 T1583 npadvmod tetracycline,responsive
R960 T1583 T1581 amod responsive,promoter
R961 T1584 T1583 punct -,responsive
R962 T1585 T1581 prep of,promoter
R963 T1586 T1585 pobj pTetSplice,of
R964 T1587 T1588 punct (,Technologies
R965 T1588 T1586 parataxis Technologies,pTetSplice
R966 T1589 T1588 compound Life,Technologies
R967 T1590 T1588 punct ", ",Technologies
R968 T1591 T1588 npadvmod Rockville,Technologies
R969 T1592 T1588 punct ", ",Technologies
R970 T1593 T1588 npadvmod Maryland,Technologies
R971 T1594 T1588 punct ", ",Technologies
R972 T1595 T1596 compound United,States
R973 T1596 T1588 npadvmod States,Technologies
R974 T1597 T1588 punct ),Technologies
R975 T1598 T1558 punct ", ",replacing
R976 T1599 T1558 cc and,replacing
R977 T1600 T1601 advmod then,ligating
R978 T1601 T1558 conj ligating,replacing
R979 T1602 T1603 compound mouse,APP
R980 T1603 T1601 dobj APP,ligating
R981 T1604 T1601 prep with,ligating
R982 T1605 T1606 det a,domain
R983 T1606 T1609 nmod domain,cDNA
R984 T1607 T1606 amod humanized,domain
R985 T1608 T1606 nmod Aβ,domain
R986 T1609 T1604 pobj cDNA,with
R987 T1610 T1606 punct (,domain
R988 T1611 T1612 compound mo,huAPP695
R989 T1612 T1606 appos huAPP695,domain
R990 T1613 T1612 punct /,huAPP695
R991 T1614 T1609 punct ),cDNA
R992 T1615 T1601 prep into,ligating
R993 T1616 T1617 det the,vector
R994 T1617 T1615 pobj vector,into
R995 T1618 T1617 amod new,vector
R996 T1619 T1539 punct .,created
R997 T1621 T1622 nsubj We,began
R998 T1623 T1622 prep by,began
R999 T1624 T1623 pcomp cloning,by