PMC:1283364 / 5484-21072 JSON TXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T1535	9-18	NN	denotes	Transgene
T1536	19-31	NN	denotes	Construction
T1537	31-431	sentence	denotes	We created a tetracycline-responsive chimeric mouse/human APP695Swedish/Indiana (swe/ind) vector by replacing the promoter region of the moPrP.XhoI vector (also known as pPrPpE1/E2,3sal [15]) with the tetracycline-responsive promoter of pTetSplice (Life Technologies, Rockville, Maryland, United States), and then ligating mouse APP with a humanized Aβ domain (mo/huAPP695) cDNA into the new vector.
T1538	32-34	PRP	denotes	We
T1539	35-42	VBD	denotes	created
T1540	43-44	DT	denotes	a
T1542	45-57	NN	denotes	tetracycline
T1544	57-58	HYPH	denotes	-
T1543	58-68	JJ	denotes	responsive
T1545	69-77	JJ	denotes	chimeric
T1546	78-83	NN	denotes	mouse
T1547	83-84	HYPH	denotes	/
T1548	84-89	JJ	denotes	human
T1549	90-103	NN	denotes	APP695Swedish
T1551	103-104	HYPH	denotes	/
T1550	104-111	NNP	denotes	Indiana
T1552	112-113	-LRB-	denotes	(
T1553	113-116	NN	denotes	swe
T1555	116-117	HYPH	denotes	/
T1554	117-120	NN	denotes	ind
T1556	120-121	-RRB-	denotes	)
T1541	122-128	NN	denotes	vector
T1557	129-131	IN	denotes	by
T1558	132-141	VBG	denotes	replacing
T1559	142-145	DT	denotes	the
T1561	146-154	NN	denotes	promoter
T1560	155-161	NN	denotes	region
T1562	162-164	IN	denotes	of
T1563	165-168	DT	denotes	the
T1565	169-179	NN	denotes	moPrP.XhoI
T1564	180-186	NN	denotes	vector
T1566	187-188	-LRB-	denotes	(
T1568	188-192	RB	denotes	also
T1567	193-198	VBN	denotes	known
T1569	199-201	IN	denotes	as
T1570	202-209	NN	denotes	pPrPpE1
T1572	209-210	HYPH	denotes	/
T1573	210-212	NN	denotes	E2
T1574	212-213	,	denotes	,
T1571	213-217	NN	denotes	3sal
T1575	218-219	-LRB-	denotes	[
T1576	219-221	CD	denotes	15
T1577	221-222	-RRB-	denotes	]
T1578	222-223	-RRB-	denotes	)
T1579	224-228	IN	denotes	with
T1580	229-232	DT	denotes	the
T1582	233-245	NN	denotes	tetracycline
T1584	245-246	HYPH	denotes	-
T1583	246-256	JJ	denotes	responsive
T1581	257-265	NN	denotes	promoter
T1585	266-268	IN	denotes	of
T1586	269-279	NN	denotes	pTetSplice
T1587	280-281	-LRB-	denotes	(
T1589	281-285	NNP	denotes	Life
T1588	286-298	NNP	denotes	Technologies
T1590	298-300	,	denotes	,
T1591	300-309	NNP	denotes	Rockville
T1592	309-311	,	denotes	,
T1593	311-319	NNP	denotes	Maryland
T1594	319-321	,	denotes	,
T1595	321-327	NNP	denotes	United
T1596	328-334	NNP	denotes	States
T1597	334-335	-RRB-	denotes	)
T1598	335-337	,	denotes	,
T1599	337-340	CC	denotes	and
T1600	341-345	RB	denotes	then
T1601	346-354	VBG	denotes	ligating
T1602	355-360	NN	denotes	mouse
T1603	361-364	NN	denotes	APP
T1604	365-369	IN	denotes	with
T1605	370-371	DT	denotes	a
T1607	372-381	JJ	denotes	humanized
T1608	382-384	NN	denotes	Aβ
T1606	385-391	NN	denotes	domain
T1610	392-393	-LRB-	denotes	(
T1611	393-395	NN	denotes	mo
T1613	395-396	HYPH	denotes	/
T1612	396-404	NN	denotes	huAPP695
T1614	404-405	-RRB-	denotes	)
T1609	406-410	NN	denotes	cDNA
T1615	411-415	IN	denotes	into
T1616	416-419	DT	denotes	the
T1618	420-423	JJ	denotes	new
T1617	424-430	NN	denotes	vector
T1619	430-431	.	denotes	.
T1620	431-801	sentence	denotes	We began by cloning the tetracycline-responsive promoter (bp 6–481) from pTetSplice by PCR using primers that added external BamHI and NotI sites to the 5′ end and a BamHI site to the 3′ end, while destroying XhoI and BamHI sites within the promoter (forward: GCC GGA TCC GCG GCC GCC GTC GAG TTT ACC ACT CCC TAT C; reverse: GCC GGA TCC ACT CTA GAA GAT CCC CGG GTA CCG).
T1621	432-434	PRP	denotes	We
T1622	435-440	VBD	denotes	began
T1623	441-443	IN	denotes	by
T1624	444-451	VBG	denotes	cloning
T1625	452-455	DT	denotes	the
T1627	456-468	NN	denotes	tetracycline
T1629	468-469	HYPH	denotes	-
T1628	469-479	JJ	denotes	responsive
T1626	480-488	NN	denotes	promoter
T1630	489-490	-LRB-	denotes	(
T1632	490-492	NN	denotes	bp
T1631	493-494	CD	denotes	6
T1633	494-495	SYM	denotes	–
T1634	495-498	CD	denotes	481
T1635	498-499	-RRB-	denotes	)
T1636	500-504	IN	denotes	from
T1637	505-515	NN	denotes	pTetSplice
T1638	516-518	IN	denotes	by
T1639	519-522	NN	denotes	PCR
T1640	523-528	VBG	denotes	using
T1641	529-536	NNS	denotes	primers
T1642	537-541	WDT	denotes	that
T1643	542-547	VBD	denotes	added
T1644	548-556	JJ	denotes	external
T1646	557-562	NN	denotes	BamHI
T1647	563-566	CC	denotes	and
T1648	567-571	NN	denotes	NotI
T1645	572-577	NNS	denotes	sites
T1649	578-580	IN	denotes	to
T1650	581-584	DT	denotes	the
T1652	585-586	CD	denotes	5
T1653	586-587	SYM	denotes	′
T1651	588-591	NN	denotes	end
T1654	592-595	CC	denotes	and
T1655	596-597	DT	denotes	a
T1657	598-603	NN	denotes	BamHI
T1656	604-608	NN	denotes	site
T1658	609-611	IN	denotes	to
T1659	612-615	DT	denotes	the
T1661	616-617	CD	denotes	3
T1662	617-618	SYM	denotes	′
T1660	619-622	NN	denotes	end
T1663	622-624	,	denotes	,
T1664	624-629	IN	denotes	while
T1665	630-640	VBG	denotes	destroying
T1666	641-645	NN	denotes	XhoI
T1668	646-649	CC	denotes	and
T1669	650-655	NN	denotes	BamHI
T1667	656-661	NNS	denotes	sites
T1670	662-668	IN	denotes	within
T1671	669-672	DT	denotes	the
T1672	673-681	NN	denotes	promoter
T1673	682-683	-LRB-	denotes	(
T1675	683-690	NN	denotes	forward
T1677	690-692	:	denotes	:
T1678	692-695	NN	denotes	GCC
T1679	696-699	NN	denotes	GGA
T1680	700-703	NN	denotes	TCC
T1681	704-707	NN	denotes	GCG
T1682	708-711	NN	denotes	GCC
T1683	712-715	NN	denotes	GCC
T1684	716-719	NN	denotes	GTC
T1685	720-723	NN	denotes	GAG
T1686	724-727	NN	denotes	TTT
T1687	728-731	NN	denotes	ACC
T1688	732-735	NN	denotes	ACT
T1689	736-739	NN	denotes	CCC
T1690	740-743	NN	denotes	TAT
T1676	744-745	NN	denotes	C
T1691	745-746	:	denotes	;
T1692	747-754	NN	denotes	reverse
T1693	754-756	:	denotes	:
T1694	756-759	NN	denotes	GCC
T1695	760-763	NN	denotes	GGA
T1696	764-767	NN	denotes	TCC
T1697	768-771	NN	denotes	ACT
T1698	772-775	NN	denotes	CTA
T1699	776-779	NN	denotes	GAA
T1700	780-783	NN	denotes	GAT
T1701	784-787	NN	denotes	CCC
T1702	788-791	NN	denotes	CGG
T1703	792-795	NN	denotes	GTA
T1674	796-799	NN	denotes	CCG
T1704	799-800	-RRB-	denotes	)
T1705	800-801	.	denotes	.
T1706	801-1067	sentence	denotes	We then isolated the moPrP.XhoI intron by amplification with primers that added an external BamHI site to the 5′ end of exon 1 and ran through the Asp718 site of exon 2 (forward: GCC GGA TCC GAT CAG CAG ACC GAT TCT GG; reverse: GCC GGT ACC ACT AGG AAG GCA GAA TGC).
T1707	802-804	PRP	denotes	We
T1709	805-809	RB	denotes	then
T1708	810-818	VBD	denotes	isolated
T1710	819-822	DT	denotes	the
T1712	823-833	NN	denotes	moPrP.XhoI
T1711	834-840	NN	denotes	intron
T1713	841-843	IN	denotes	by
T1714	844-857	NN	denotes	amplification
T1715	858-862	IN	denotes	with
T1716	863-870	NNS	denotes	primers
T1717	871-875	WDT	denotes	that
T1718	876-881	VBD	denotes	added
T1719	882-884	DT	denotes	an
T1721	885-893	JJ	denotes	external
T1722	894-899	NN	denotes	BamHI
T1720	900-904	NN	denotes	site
T1723	905-907	IN	denotes	to
T1724	908-911	DT	denotes	the
T1726	912-913	CD	denotes	5
T1727	913-914	SYM	denotes	′
T1725	915-918	NN	denotes	end
T1728	919-921	IN	denotes	of
T1729	922-926	NN	denotes	exon
T1730	927-928	CD	denotes	1
T1731	929-932	CC	denotes	and
T1732	933-936	VBD	denotes	ran
T1733	937-944	IN	denotes	through
T1734	945-948	DT	denotes	the
T1736	949-955	NN	denotes	Asp718
T1735	956-960	NN	denotes	site
T1737	961-963	IN	denotes	of
T1738	964-968	NN	denotes	exon
T1739	969-970	CD	denotes	2
T1740	971-972	-LRB-	denotes	(
T1742	972-979	NN	denotes	forward
T1744	979-981	:	denotes	:
T1745	981-984	NN	denotes	GCC
T1746	985-988	NN	denotes	GGA
T1747	989-992	NN	denotes	TCC
T1748	993-996	NN	denotes	GAT
T1749	997-1000	NN	denotes	CAG
T1750	1001-1004	NN	denotes	CAG
T1751	1005-1008	NN	denotes	ACC
T1752	1009-1012	NN	denotes	GAT
T1753	1013-1016	NN	denotes	TCT
T1743	1017-1019	NN	denotes	GG
T1754	1019-1020	:	denotes	;
T1755	1021-1028	NN	denotes	reverse
T1756	1028-1030	:	denotes	:
T1757	1030-1033	NN	denotes	GCC
T1758	1034-1037	NN	denotes	GGT
T1759	1038-1041	NN	denotes	ACC
T1760	1042-1045	NN	denotes	ACT
T1761	1046-1049	NN	denotes	AGG
T1762	1050-1053	NN	denotes	AAG
T1763	1054-1057	NN	denotes	GCA
T1764	1058-1061	NN	denotes	GAA
T1741	1062-1065	NN	denotes	TGC
T1765	1065-1066	-RRB-	denotes	)
T1766	1066-1067	.	denotes	.
T1767	1067-1418	sentence	denotes	This 2-kb intron fragment was cloned into a TA cloning vector (Invitrogen, Carlsbad, California, United States), then excised by Asp718 digestion and ligated to the 6.8-kb Asp718 fragment of moPrP.XhoI containing exon 2, exon 3, the 3′ UTR, and pBluescript to generate an intermediate vector with all three exons and a central intron but no promoter.
T1768	1068-1072	DT	denotes	This
T1770	1073-1074	CD	denotes	2
T1772	1074-1075	HYPH	denotes	-
T1771	1075-1077	NN	denotes	kb
T1773	1078-1084	NN	denotes	intron
T1769	1085-1093	NN	denotes	fragment
T1775	1094-1097	VBD	denotes	was
T1774	1098-1104	VBN	denotes	cloned
T1776	1105-1109	IN	denotes	into
T1777	1110-1111	DT	denotes	a
T1779	1112-1114	NN	denotes	TA
T1780	1115-1122	NN	denotes	cloning
T1778	1123-1129	NN	denotes	vector
T1781	1130-1131	-LRB-	denotes	(
T1782	1131-1141	NNP	denotes	Invitrogen
T1783	1141-1143	,	denotes	,
T1784	1143-1151	NNP	denotes	Carlsbad
T1785	1151-1153	,	denotes	,
T1786	1153-1163	NNP	denotes	California
T1787	1163-1165	,	denotes	,
T1788	1165-1171	NNP	denotes	United
T1789	1172-1178	NNP	denotes	States
T1790	1178-1179	-RRB-	denotes	)
T1791	1179-1181	,	denotes	,
T1792	1181-1185	RB	denotes	then
T1793	1186-1193	VBN	denotes	excised
T1794	1194-1196	IN	denotes	by
T1795	1197-1203	NN	denotes	Asp718
T1796	1204-1213	NN	denotes	digestion
T1797	1214-1217	CC	denotes	and
T1798	1218-1225	VBN	denotes	ligated
T1799	1226-1228	IN	denotes	to
T1800	1229-1232	DT	denotes	the
T1802	1233-1236	CD	denotes	6.8
T1804	1236-1237	HYPH	denotes	-
T1803	1237-1239	NN	denotes	kb
T1805	1240-1246	NN	denotes	Asp718
T1801	1247-1255	NN	denotes	fragment
T1806	1256-1258	IN	denotes	of
T1807	1259-1269	NN	denotes	moPrP.XhoI
T1808	1270-1280	VBG	denotes	containing
T1809	1281-1285	NN	denotes	exon
T1810	1286-1287	CD	denotes	2
T1811	1287-1289	,	denotes	,
T1812	1289-1293	NN	denotes	exon
T1813	1294-1295	CD	denotes	3
T1814	1295-1297	,	denotes	,
T1815	1297-1300	DT	denotes	the
T1817	1301-1302	CD	denotes	3
T1818	1302-1303	SYM	denotes	′
T1816	1304-1307	NN	denotes	UTR
T1819	1307-1309	,	denotes	,
T1820	1309-1312	CC	denotes	and
T1821	1313-1324	NN	denotes	pBluescript
T1822	1325-1327	TO	denotes	to
T1823	1328-1336	VB	denotes	generate
T1824	1337-1339	DT	denotes	an
T1826	1340-1352	JJ	denotes	intermediate
T1825	1353-1359	NN	denotes	vector
T1827	1360-1364	IN	denotes	with
T1828	1365-1368	DT	denotes	all
T1830	1369-1374	CD	denotes	three
T1829	1375-1380	NNS	denotes	exons
T1831	1381-1384	CC	denotes	and
T1832	1385-1386	DT	denotes	a
T1834	1387-1394	JJ	denotes	central
T1833	1395-1401	NN	denotes	intron
T1835	1402-1405	CC	denotes	but
T1836	1406-1408	DT	denotes	no
T1837	1409-1417	NN	denotes	promoter
T1838	1417-1418	.	denotes	.
T1839	1418-1573	sentence	denotes	This vector was then opened at the BamHI site introduced by the intron cloning primer, and ligated to the 0.5-kb BamHI-cut tetracycline promoter fragment.
T1840	1419-1423	DT	denotes	This
T1841	1424-1430	NN	denotes	vector
T1843	1431-1434	VBD	denotes	was
T1844	1435-1439	RB	denotes	then
T1842	1440-1446	VBN	denotes	opened
T1845	1447-1449	IN	denotes	at
T1846	1450-1453	DT	denotes	the
T1848	1454-1459	NN	denotes	BamHI
T1847	1460-1464	NN	denotes	site
T1849	1465-1475	VBN	denotes	introduced
T1850	1476-1478	IN	denotes	by
T1851	1479-1482	DT	denotes	the
T1853	1483-1489	NN	denotes	intron
T1854	1490-1497	NN	denotes	cloning
T1852	1498-1504	NN	denotes	primer
T1855	1504-1506	,	denotes	,
T1856	1506-1509	CC	denotes	and
T1857	1510-1517	VBN	denotes	ligated
T1858	1518-1520	IN	denotes	to
T1859	1521-1524	DT	denotes	the
T1861	1525-1528	CD	denotes	0.5
T1863	1528-1529	HYPH	denotes	-
T1862	1529-1531	NN	denotes	kb
T1864	1532-1537	NN	denotes	BamHI
T1866	1537-1538	HYPH	denotes	-
T1865	1538-1541	NN	denotes	cut
T1867	1542-1554	NN	denotes	tetracycline
T1868	1555-1563	NN	denotes	promoter
T1860	1564-1572	NN	denotes	fragment
T1869	1572-1573	.	denotes	.
T1870	1573-1789	sentence	denotes	This ligation generated a 9.3-kb vector encoding the tetracycline promoter from pTetSplice with two exons, one intron, and the original 3′ UTR of the moPrP.XhoI vector, all carried in the pBluescript cloning vector.
T1871	1574-1578	DT	denotes	This
T1872	1579-1587	NN	denotes	ligation
T1873	1588-1597	VBD	denotes	generated
T1874	1598-1599	DT	denotes	a
T1876	1600-1603	CD	denotes	9.3
T1878	1603-1604	HYPH	denotes	-
T1877	1604-1606	NN	denotes	kb
T1875	1607-1613	NN	denotes	vector
T1879	1614-1622	VBG	denotes	encoding
T1880	1623-1626	DT	denotes	the
T1882	1627-1639	NN	denotes	tetracycline
T1881	1640-1648	NN	denotes	promoter
T1883	1649-1653	IN	denotes	from
T1884	1654-1664	NN	denotes	pTetSplice
T1885	1665-1669	IN	denotes	with
T1886	1670-1673	CD	denotes	two
T1887	1674-1679	NNS	denotes	exons
T1888	1679-1681	,	denotes	,
T1889	1681-1684	CD	denotes	one
T1890	1685-1691	NN	denotes	intron
T1891	1691-1693	,	denotes	,
T1892	1693-1696	CC	denotes	and
T1893	1697-1700	DT	denotes	the
T1895	1701-1709	JJ	denotes	original
T1896	1710-1711	CD	denotes	3
T1897	1711-1712	SYM	denotes	′
T1894	1713-1716	NN	denotes	UTR
T1898	1717-1719	IN	denotes	of
T1899	1720-1723	DT	denotes	the
T1901	1724-1734	NN	denotes	moPrP.XhoI
T1900	1735-1741	NN	denotes	vector
T1902	1741-1743	,	denotes	,
T1903	1743-1746	RB	denotes	all
T1904	1747-1754	VBN	denotes	carried
T1905	1755-1757	IN	denotes	in
T1906	1758-1761	DT	denotes	the
T1908	1762-1773	NNP	denotes	pBluescript
T1909	1774-1781	NN	denotes	cloning
T1907	1782-1788	NN	denotes	vector
T1910	1788-1789	.	denotes	.
T1911	1789-2239	sentence	denotes	We incorporated the Swedish (KM570/571NL) and Indiana (V617F) mutations into the mo/huAPP695 cDNA (in BS-KS) by PCR using a four-primer strategy: first, two partially overlapping products were generated in separate reactions using primers that encode the desired mutations (Swedish forward: GGA GAT CTC TGA AGT GAA TCT GGA TGC AGA ATT CCG/Indiana reverse: GGG TGA TGA AAA TCA CGG TTG C; Indiana forward: CAA CCG TGA TTT TCA TCA CCC TGG/M13 reverse).
T1912	1790-1792	PRP	denotes	We
T1913	1793-1805	VBD	denotes	incorporated
T1915	1806-1809	DT	denotes	the
T1917	1810-1817	NNP	denotes	Swedish
T1918	1818-1819	-LRB-	denotes	(
T1920	1819-1824	NN	denotes	KM570
T1921	1824-1825	HYPH	denotes	/
T1919	1825-1830	NN	denotes	571NL
T1922	1830-1831	-RRB-	denotes	)
T1923	1832-1835	CC	denotes	and
T1924	1836-1843	NNP	denotes	Indiana
T1925	1844-1845	-LRB-	denotes	(
T1926	1845-1850	NN	denotes	V617F
T1927	1850-1851	-RRB-	denotes	)
T1916	1852-1861	NNS	denotes	mutations
T1928	1862-1866	IN	denotes	into
T1929	1867-1870	DT	denotes	the
T1931	1871-1873	NN	denotes	mo
T1933	1873-1874	HYPH	denotes	/
T1932	1874-1882	NN	denotes	huAPP695
T1930	1883-1887	NN	denotes	cDNA
T1934	1888-1889	-LRB-	denotes	(
T1935	1889-1891	IN	denotes	in
T1936	1892-1894	NN	denotes	BS
T1938	1894-1895	HYPH	denotes	-
T1937	1895-1897	NN	denotes	KS
T1939	1897-1898	-RRB-	denotes	)
T1940	1899-1901	IN	denotes	by
T1941	1902-1905	NN	denotes	PCR
T1942	1906-1911	VBG	denotes	using
T1943	1912-1913	DT	denotes	a
T1945	1914-1918	CD	denotes	four
T1947	1918-1919	HYPH	denotes	-
T1946	1919-1925	NN	denotes	primer
T1944	1926-1934	NN	denotes	strategy
T1948	1934-1936	:	denotes	:
T1949	1936-1941	RB	denotes	first
T1950	1941-1943	,	denotes	,
T1951	1943-1946	CD	denotes	two
T1953	1947-1956	RB	denotes	partially
T1954	1957-1968	VBG	denotes	overlapping
T1952	1969-1977	NNS	denotes	products
T1955	1978-1982	VBD	denotes	were
T1914	1983-1992	VBN	denotes	generated
T1956	1993-1995	IN	denotes	in
T1957	1996-2004	JJ	denotes	separate
T1958	2005-2014	NNS	denotes	reactions
T1959	2015-2020	VBG	denotes	using
T1960	2021-2028	NNS	denotes	primers
T1961	2029-2033	WDT	denotes	that
T1962	2034-2040	VBP	denotes	encode
T1963	2041-2044	DT	denotes	the
T1965	2045-2052	VBN	denotes	desired
T1964	2053-2062	NNS	denotes	mutations
T1966	2063-2064	-LRB-	denotes	(
T1968	2064-2071	NNP	denotes	Swedish
T1969	2072-2079	NN	denotes	forward
T1971	2079-2081	:	denotes	:
T1972	2081-2084	NN	denotes	GGA
T1973	2085-2088	NN	denotes	GAT
T1974	2089-2092	NN	denotes	CTC
T1975	2093-2096	NN	denotes	TGA
T1976	2097-2100	NN	denotes	AGT
T1977	2101-2104	NN	denotes	GAA
T1978	2105-2108	NN	denotes	TCT
T1979	2109-2112	NNP	denotes	GGA
T1980	2113-2116	NNP	denotes	TGC
T1981	2117-2120	NNP	denotes	AGA
T1982	2121-2124	NNP	denotes	ATT
T1970	2125-2128	NN	denotes	CCG
T1984	2128-2129	HYPH	denotes	/
T1985	2129-2136	NNP	denotes	Indiana
T1986	2137-2144	NN	denotes	reverse
T1987	2144-2146	:	denotes	:
T1988	2146-2149	NN	denotes	GGG
T1989	2150-2153	NN	denotes	TGA
T1990	2154-2157	NN	denotes	TGA
T1991	2158-2161	NN	denotes	AAA
T1992	2162-2165	NN	denotes	TCA
T1993	2166-2169	NN	denotes	CGG
T1994	2170-2173	NN	denotes	TTG
T1983	2174-2175	NN	denotes	C
T1995	2175-2176	:	denotes	;
T1996	2177-2184	NNP	denotes	Indiana
T1997	2185-2192	NN	denotes	forward
T1998	2192-2194	:	denotes	:
T1999	2194-2197	NN	denotes	CAA
T2000	2198-2201	NN	denotes	CCG
T2001	2202-2205	NN	denotes	TGA
T2002	2206-2209	NN	denotes	TTT
T2003	2210-2213	NN	denotes	TCA
T2004	2214-2217	NN	denotes	TCA
T2005	2218-2221	NN	denotes	CCC
T2006	2222-2225	NN	denotes	TGG
T2007	2225-2226	HYPH	denotes	/
T1967	2226-2229	NN	denotes	M13
T2008	2230-2237	RB	denotes	reverse
T2009	2237-2238	-RRB-	denotes	)
T2010	2238-2239	.	denotes	.
T2011	2239-2363	sentence	denotes	The two PCR products were ligated, digested with BglII and ApaI and cloned back into the original mo/huAPP695-BS-KS vector.
T2012	2240-2243	DT	denotes	The
T2014	2244-2247	CD	denotes	two
T2015	2248-2251	NN	denotes	PCR
T2013	2252-2260	NNS	denotes	products
T2017	2261-2265	VBD	denotes	were
T2016	2266-2273	VBN	denotes	ligated
T2018	2273-2275	,	denotes	,
T2019	2275-2283	VBN	denotes	digested
T2020	2284-2288	IN	denotes	with
T2021	2289-2294	NN	denotes	BglII
T2022	2295-2298	CC	denotes	and
T2023	2299-2303	NN	denotes	ApaI
T2024	2304-2307	CC	denotes	and
T2025	2308-2314	VBN	denotes	cloned
T2026	2315-2319	RB	denotes	back
T2027	2320-2324	IN	denotes	into
T2028	2325-2328	DT	denotes	the
T2030	2329-2337	JJ	denotes	original
T2031	2338-2340	NN	denotes	mo
T2033	2340-2341	HYPH	denotes	/
T2034	2341-2349	NN	denotes	huAPP695
T2035	2349-2350	HYPH	denotes	-
T2036	2350-2352	NN	denotes	BS
T2037	2352-2353	HYPH	denotes	-
T2032	2353-2355	NN	denotes	KS
T2029	2356-2362	NN	denotes	vector
T2038	2362-2363	.	denotes	.
T2039	2363-2490	sentence	denotes	Finally, the new APP695swe/ind was subcloned into the XhoI site of the moPrP-tetP vector from above to complete the construct.
T2040	2364-2371	RB	denotes	Finally
T2042	2371-2373	,	denotes	,
T2043	2373-2376	DT	denotes	the
T2045	2377-2380	JJ	denotes	new
T2046	2381-2390	NN	denotes	APP695swe
T2047	2390-2391	HYPH	denotes	/
T2044	2391-2394	NN	denotes	ind
T2048	2395-2398	VBD	denotes	was
T2041	2399-2408	VBN	denotes	subcloned
T2049	2409-2413	IN	denotes	into
T2050	2414-2417	DT	denotes	the
T2052	2418-2422	NN	denotes	XhoI
T2051	2423-2427	NN	denotes	site
T2053	2428-2430	IN	denotes	of
T2054	2431-2434	DT	denotes	the
T2056	2435-2440	NN	denotes	moPrP
T2058	2440-2441	HYPH	denotes	-
T2057	2441-2445	NN	denotes	tetP
T2055	2446-2452	NN	denotes	vector
T2059	2453-2457	IN	denotes	from
T2060	2458-2463	RB	denotes	above
T2061	2464-2466	TO	denotes	to
T2062	2467-2475	VB	denotes	complete
T2063	2476-2479	DT	denotes	the
T2064	2480-2489	NN	denotes	construct
T2065	2489-2490	.	denotes	.
T2158	2492-2502	JJ	denotes	Pronuclear
T2159	2503-2512	NN	denotes	Injection
T2160	2512-2514	,	denotes	,
T2161	2514-2523	NN	denotes	Screening
T2162	2524-2526	IN	denotes	of
T2163	2527-2535	NNS	denotes	Founders
T2164	2535-2537	,	denotes	,
T2165	2537-2540	CC	denotes	and
T2166	2541-2552	NN	denotes	Maintenance
T2167	2553-2555	IN	denotes	of
T2168	2556-2559	DT	denotes	the
T2169	2560-2565	NNS	denotes	Lines
T2170	2565-2680	sentence	denotes	The moPrP-tetP-mo/huAPP695swe/ind vector was linearized and the pBluescript domain excised by digestion with NotI.
T2171	2566-2569	DT	denotes	The
T2173	2570-2575	NN	denotes	moPrP
T2175	2575-2576	HYPH	denotes	-
T2176	2576-2580	NN	denotes	tetP
T2177	2580-2581	HYPH	denotes	-
T2178	2581-2583	NN	denotes	mo
T2179	2583-2584	HYPH	denotes	/
T2180	2584-2595	NN	denotes	huAPP695swe
T2181	2595-2596	HYPH	denotes	/
T2174	2596-2599	NN	denotes	ind
T2172	2600-2606	NN	denotes	vector
T2183	2607-2610	VBD	denotes	was
T2182	2611-2621	VBN	denotes	linearized
T2184	2622-2625	CC	denotes	and
T2185	2626-2629	DT	denotes	the
T2187	2630-2641	NNP	denotes	pBluescript
T2186	2642-2648	NN	denotes	domain
T2188	2649-2656	VBN	denotes	excised
T2189	2657-2659	IN	denotes	by
T2190	2660-2669	NN	denotes	digestion
T2191	2670-2674	IN	denotes	with
T2192	2675-2679	NN	denotes	NotI
T2193	2679-2680	.	denotes	.
T2194	2680-2786	sentence	denotes	The purified vector was injected into the pronucleus of fertilized eggs from C57BL/6J × C3HeJ F1 matings.
T2195	2681-2684	DT	denotes	The
T2197	2685-2693	VBN	denotes	purified
T2196	2694-2700	NN	denotes	vector
T2199	2701-2704	VBD	denotes	was
T2198	2705-2713	VBN	denotes	injected
T2200	2714-2718	IN	denotes	into
T2201	2719-2722	DT	denotes	the
T2202	2723-2733	NN	denotes	pronucleus
T2203	2734-2736	IN	denotes	of
T2204	2737-2747	VBN	denotes	fertilized
T2205	2748-2752	NNS	denotes	eggs
T2206	2753-2757	IN	denotes	from
T2207	2758-2763	NN	denotes	C57BL
T2209	2763-2764	HYPH	denotes	/
T2210	2764-2766	NN	denotes	6J
T2211	2767-2768	SYM	denotes	×
T2208	2769-2774	NN	denotes	C3HeJ
T2213	2775-2777	NN	denotes	F1
T2212	2778-2785	NNS	denotes	matings
T2214	2785-2786	.	denotes	.
T2215	2786-2923	sentence	denotes	Founder animals were screened for the presence of the transgene by three-way PCR using the S36 and PrP-S/PrP-AS primers described below.
T2216	2787-2794	NN	denotes	Founder
T2217	2795-2802	NNS	denotes	animals
T2219	2803-2807	VBD	denotes	were
T2218	2808-2816	VBN	denotes	screened
T2220	2817-2820	IN	denotes	for
T2221	2821-2824	DT	denotes	the
T2222	2825-2833	NN	denotes	presence
T2223	2834-2836	IN	denotes	of
T2224	2837-2840	DT	denotes	the
T2225	2841-2850	NN	denotes	transgene
T2226	2851-2853	IN	denotes	by
T2227	2854-2859	CD	denotes	three
T2229	2859-2860	HYPH	denotes	-
T2228	2860-2863	NN	denotes	way
T2230	2864-2867	NN	denotes	PCR
T2231	2868-2873	VBG	denotes	using
T2232	2874-2877	DT	denotes	the
T2234	2878-2881	NN	denotes	S36
T2235	2882-2885	CC	denotes	and
T2236	2886-2889	NN	denotes	PrP
T2238	2889-2890	HYPH	denotes	-
T2239	2890-2891	NN	denotes	S
T2240	2891-2892	HYPH	denotes	/
T2241	2892-2895	NN	denotes	PrP
T2242	2895-2896	HYPH	denotes	-
T2237	2896-2898	NN	denotes	AS
T2233	2899-2906	NNS	denotes	primers
T2243	2907-2916	VBN	denotes	described
T2244	2917-2922	RB	denotes	below
T2245	2922-2923	.	denotes	.
T2246	2923-3208	sentence	denotes	Transgene-positive founders were bred to animals expressing the tetracycline transactivator (tTA) under control of the calcium-calmodulin kinase IIα (CaMKIIα) promoter obtained from Jackson Laboratory [16] (Bar Harbor, Maine, United States; stock # 3010; B6;CBA-TgN[Camk2a-tTA]1Mmay).
T2247	2924-2933	NN	denotes	Transgene
T2249	2933-2934	HYPH	denotes	-
T2248	2934-2942	JJ	denotes	positive
T2250	2943-2951	NNS	denotes	founders
T2252	2952-2956	VBD	denotes	were
T2251	2957-2961	VBN	denotes	bred
T2253	2962-2964	IN	denotes	to
T2254	2965-2972	NNS	denotes	animals
T2255	2973-2983	VBG	denotes	expressing
T2256	2984-2987	DT	denotes	the
T2258	2988-3000	NN	denotes	tetracycline
T2257	3001-3015	NN	denotes	transactivator
T2259	3016-3017	-LRB-	denotes	(
T2260	3017-3020	NN	denotes	tTA
T2261	3020-3021	-RRB-	denotes	)
T2262	3022-3027	IN	denotes	under
T2263	3028-3035	NN	denotes	control
T2264	3036-3038	IN	denotes	of
T2265	3039-3042	DT	denotes	the
T2267	3043-3050	NN	denotes	calcium
T2269	3050-3051	HYPH	denotes	-
T2268	3051-3061	NN	denotes	calmodulin
T2271	3062-3068	NN	denotes	kinase
T2270	3069-3072	NN	denotes	IIα
T2272	3073-3074	-LRB-	denotes	(
T2273	3074-3081	NN	denotes	CaMKIIα
T2274	3081-3082	-RRB-	denotes	)
T2266	3083-3091	NN	denotes	promoter
T2275	3092-3100	VBN	denotes	obtained
T2276	3101-3105	IN	denotes	from
T2277	3106-3113	NNP	denotes	Jackson
T2278	3114-3124	NNP	denotes	Laboratory
T2279	3125-3126	-LRB-	denotes	[
T2280	3126-3128	CD	denotes	16
T2281	3128-3129	-RRB-	denotes	]
T2282	3130-3131	-LRB-	denotes	(
T2284	3131-3134	NNP	denotes	Bar
T2283	3135-3141	NNP	denotes	Harbor
T2285	3141-3143	,	denotes	,
T2286	3143-3148	NNP	denotes	Maine
T2287	3148-3150	,	denotes	,
T2288	3150-3156	NNP	denotes	United
T2289	3157-3163	NNP	denotes	States
T2290	3163-3164	:	denotes	;
T2291	3165-3170	NN	denotes	stock
T2293	3171-3172	SYM	denotes	#
T2294	3173-3177	CD	denotes	3010
T2295	3177-3178	:	denotes	;
T2296	3179-3181	NN	denotes	B6
T2297	3181-3182	:	denotes	;
T2298	3182-3185	NN	denotes	CBA
T2299	3185-3186	HYPH	denotes	-
T2300	3186-3189	NN	denotes	TgN
T2301	3189-3190	-LRB-	denotes	[
T2302	3190-3196	NN	denotes	Camk2a
T2303	3196-3197	HYPH	denotes	-
T2304	3197-3200	NN	denotes	tTA
T2305	3200-3201	-RRB-	denotes	]
T2292	3201-3206	NN	denotes	1Mmay
T2306	3206-3207	-RRB-	denotes	)
T2307	3207-3208	.	denotes	.
T2308	3208-3329	sentence	denotes	The colony was thereafter maintained by crossing single transgenic tTA and APP offspring for each of the four APP lines.
T2309	3209-3212	DT	denotes	The
T2310	3213-3219	NN	denotes	colony
T2312	3220-3223	VBD	denotes	was
T2313	3224-3234	RB	denotes	thereafter
T2311	3235-3245	VBN	denotes	maintained
T2314	3246-3248	IN	denotes	by
T2315	3249-3257	VBG	denotes	crossing
T2316	3258-3264	JJ	denotes	single
T2318	3265-3275	JJ	denotes	transgenic
T2319	3276-3279	NN	denotes	tTA
T2320	3280-3283	CC	denotes	and
T2321	3284-3287	NN	denotes	APP
T2317	3288-3297	NN	denotes	offspring
T2322	3298-3301	IN	denotes	for
T2323	3302-3306	DT	denotes	each
T2324	3307-3309	IN	denotes	of
T2325	3310-3313	DT	denotes	the
T2327	3314-3318	CD	denotes	four
T2328	3319-3322	NN	denotes	APP
T2326	3323-3328	NNS	denotes	lines
T2329	3328-3329	.	denotes	.
T2330	3329-3385	sentence	denotes	All mice were provided fresh food and water ad libitum.
T2331	3330-3333	DT	denotes	All
T2332	3334-3338	NNS	denotes	mice
T2334	3339-3343	VBD	denotes	were
T2333	3344-3352	VBN	denotes	provided
T2335	3353-3358	JJ	denotes	fresh
T2336	3359-3363	NN	denotes	food
T2337	3364-3367	CC	denotes	and
T2338	3368-3373	NN	denotes	water
T2339	3374-3376	FW	denotes	ad
T2340	3377-3384	FW	denotes	libitum
T2341	3384-3385	.	denotes	.
T2342	3385-3542	sentence	denotes	Animal protocols were approved by both the Johns Hopkins University and the California Institute of Technology Institutional Animal Care and Use Committees.
T2343	3386-3392	NN	denotes	Animal
T2344	3393-3402	NNS	denotes	protocols
T2346	3403-3407	VBD	denotes	were
T2345	3408-3416	VBN	denotes	approved
T2347	3417-3419	IN	denotes	by
T2348	3420-3424	DT	denotes	both
T2350	3425-3428	DT	denotes	the
T2351	3429-3434	NNP	denotes	Johns
T2352	3435-3442	NNP	denotes	Hopkins
T2349	3443-3453	NNP	denotes	University
T2354	3454-3457	CC	denotes	and
T2355	3458-3461	DT	denotes	the
T2357	3462-3472	NNP	denotes	California
T2356	3473-3482	NNP	denotes	Institute
T2358	3483-3485	IN	denotes	of
T2359	3486-3496	NNP	denotes	Technology
T2360	3497-3510	NNP	denotes	Institutional
T2361	3511-3517	NNP	denotes	Animal
T2362	3518-3522	NNP	denotes	Care
T2363	3523-3526	CC	denotes	and
T2364	3527-3530	NNP	denotes	Use
T2353	3531-3541	NNPS	denotes	Committees
T2365	3541-3542	.	denotes	.
T2408	3544-3555	NN	denotes	Doxycycline
T2409	3556-3570	NN	denotes	Administration
T2410	3570-3706	sentence	denotes	Doxycycline (dox) was administered through commercially available dox-containing chow (BioServ, Frenchtown, New Jersey, United States).
T2411	3571-3582	NN	denotes	Doxycycline
T2413	3583-3584	-LRB-	denotes	(
T2414	3584-3587	NN	denotes	dox
T2415	3587-3588	-RRB-	denotes	)
T2416	3589-3592	VBD	denotes	was
T2412	3593-3605	VBN	denotes	administered
T2417	3606-3613	IN	denotes	through
T2418	3614-3626	RB	denotes	commercially
T2419	3627-3636	JJ	denotes	available
T2421	3637-3640	NN	denotes	dox
T2423	3640-3641	HYPH	denotes	-
T2422	3641-3651	VBG	denotes	containing
T2420	3652-3656	NN	denotes	chow
T2424	3657-3658	-LRB-	denotes	(
T2425	3658-3665	NNP	denotes	BioServ
T2426	3665-3667	,	denotes	,
T2427	3667-3677	NNP	denotes	Frenchtown
T2428	3677-3679	,	denotes	,
T2429	3679-3682	NNP	denotes	New
T2430	3683-3689	NNP	denotes	Jersey
T2431	3689-3691	,	denotes	,
T2432	3691-3697	NNP	denotes	United
T2433	3698-3704	NNP	denotes	States
T2434	3704-3705	-RRB-	denotes	)
T2435	3705-3706	.	denotes	.
T2436	3706-3862	sentence	denotes	The chow contained 200 mg/kg of antibiotic; based on estimated consumption of 5 g per mouse per day, the expected dose to each animal was 1 mg dox per day.
T2437	3707-3710	DT	denotes	The
T2438	3711-3715	NN	denotes	chow
T2439	3716-3725	VBD	denotes	contained
T2441	3726-3729	CD	denotes	200
T2442	3730-3732	NN	denotes	mg
T2443	3732-3733	SYM	denotes	/
T2444	3733-3735	NN	denotes	kg
T2445	3736-3738	IN	denotes	of
T2446	3739-3749	JJ	denotes	antibiotic
T2447	3749-3750	:	denotes	;
T2448	3751-3756	VBN	denotes	based
T2449	3757-3759	IN	denotes	on
T2450	3760-3769	VBN	denotes	estimated
T2451	3770-3781	NN	denotes	consumption
T2452	3782-3784	IN	denotes	of
T2453	3785-3786	CD	denotes	5
T2454	3787-3788	NN	denotes	g
T2455	3789-3792	IN	denotes	per
T2456	3793-3798	NN	denotes	mouse
T2457	3799-3802	IN	denotes	per
T2458	3803-3806	NN	denotes	day
T2459	3806-3808	,	denotes	,
T2460	3808-3811	DT	denotes	the
T2462	3812-3820	VBN	denotes	expected
T2461	3821-3825	NN	denotes	dose
T2463	3826-3828	IN	denotes	to
T2464	3829-3833	DT	denotes	each
T2465	3834-3840	NN	denotes	animal
T2440	3841-3844	VBD	denotes	was
T2466	3845-3846	CD	denotes	1
T2467	3847-3849	NN	denotes	mg
T2468	3850-3853	NN	denotes	dox
T2469	3854-3857	IN	denotes	per
T2470	3858-3861	NN	denotes	day
T2471	3861-3862	.	denotes	.
T2472	3862-3948	sentence	denotes	The average 25-g animal therefore received 40 μg of dox per gram body weight per day.
T2473	3863-3866	DT	denotes	The
T2475	3867-3874	JJ	denotes	average
T2476	3875-3877	CD	denotes	25
T2478	3877-3878	HYPH	denotes	-
T2477	3878-3879	NN	denotes	g
T2474	3880-3886	NN	denotes	animal
T2480	3887-3896	RB	denotes	therefore
T2479	3897-3905	VBD	denotes	received
T2481	3906-3908	CD	denotes	40
T2482	3909-3911	NN	denotes	μg
T2483	3912-3914	IN	denotes	of
T2484	3915-3918	NN	denotes	dox
T2485	3919-3922	IN	denotes	per
T2486	3923-3927	NN	denotes	gram
T2488	3928-3932	NN	denotes	body
T2487	3933-3939	NN	denotes	weight
T2489	3940-3943	IN	denotes	per
T2490	3944-3947	NN	denotes	day
T2491	3947-3948	.	denotes	.
T2492	3948-4024	sentence	denotes	Chow was changed 1–2 times per week to prevent breakdown of the antibiotic.
T2493	3949-3953	NN	denotes	Chow
T2495	3954-3957	VBD	denotes	was
T2494	3958-3965	VBN	denotes	changed
T2496	3966-3967	CD	denotes	1
T2498	3967-3968	SYM	denotes	–
T2497	3968-3969	CD	denotes	2
T2499	3970-3975	NNS	denotes	times
T2500	3976-3979	IN	denotes	per
T2501	3980-3984	NN	denotes	week
T2502	3985-3987	TO	denotes	to
T2503	3988-3995	VB	denotes	prevent
T2504	3996-4005	NN	denotes	breakdown
T2505	4006-4008	IN	denotes	of
T2506	4009-4012	DT	denotes	the
T2507	4013-4023	NN	denotes	antibiotic
T2508	4023-4024	.	denotes	.
T2635	4026-4036	NN	denotes	Genotyping
T2636	4036-4167	sentence	denotes	Offspring were genotyped for the presence of each transgene by PCR amplification of genomic DNA extracted from a 5-mm tail biopsy.
T2637	4037-4046	NN	denotes	Offspring
T2639	4047-4051	VBD	denotes	were
T2638	4052-4061	VBN	denotes	genotyped
T2640	4062-4065	IN	denotes	for
T2641	4066-4069	DT	denotes	the
T2642	4070-4078	NN	denotes	presence
T2643	4079-4081	IN	denotes	of
T2644	4082-4086	DT	denotes	each
T2645	4087-4096	NN	denotes	transgene
T2646	4097-4099	IN	denotes	by
T2647	4100-4103	NN	denotes	PCR
T2648	4104-4117	NN	denotes	amplification
T2649	4118-4120	IN	denotes	of
T2650	4121-4128	JJ	denotes	genomic
T2651	4129-4132	NN	denotes	DNA
T2652	4133-4142	VBN	denotes	extracted
T2653	4143-4147	IN	denotes	from
T2654	4148-4149	DT	denotes	a
T2656	4150-4151	CD	denotes	5
T2658	4151-4152	HYPH	denotes	-
T2657	4152-4154	NN	denotes	mm
T2659	4155-4159	NN	denotes	tail
T2655	4160-4166	NN	denotes	biopsy
T2660	4166-4167	.	denotes	.
T2661	4167-4306	sentence	denotes	Tails were heated to 95 °C for 45 min in 250 μl of 50 mM NaOH, vortexed, then neutralized with an equal volume of 0.5 M Tris-HCl (pH 5.5).
T2662	4168-4173	NNS	denotes	Tails
T2664	4174-4178	VBD	denotes	were
T2663	4179-4185	VBN	denotes	heated
T2665	4186-4188	IN	denotes	to
T2666	4189-4191	CD	denotes	95
T2667	4192-4194	NN	denotes	°C
T2668	4195-4198	IN	denotes	for
T2669	4199-4201	CD	denotes	45
T2670	4202-4205	NN	denotes	min
T2671	4206-4208	IN	denotes	in
T2672	4209-4212	CD	denotes	250
T2673	4213-4215	NN	denotes	μl
T2674	4216-4218	IN	denotes	of
T2675	4219-4221	CD	denotes	50
T2676	4222-4224	NN	denotes	mM
T2677	4225-4229	NN	denotes	NaOH
T2678	4229-4231	,	denotes	,
T2679	4231-4239	VBN	denotes	vortexed
T2680	4239-4241	,	denotes	,
T2681	4241-4245	RB	denotes	then
T2682	4246-4257	VBN	denotes	neutralized
T2683	4258-4262	IN	denotes	with
T2684	4263-4265	DT	denotes	an
T2686	4266-4271	JJ	denotes	equal
T2685	4272-4278	NN	denotes	volume
T2687	4279-4281	IN	denotes	of
T2688	4282-4285	CD	denotes	0.5
T2689	4286-4287	NN	denotes	M
T2691	4288-4292	NN	denotes	Tris
T2692	4292-4293	HYPH	denotes	-
T2690	4293-4296	NN	denotes	HCl
T2693	4297-4298	-LRB-	denotes	(
T2694	4298-4300	NN	denotes	pH
T2695	4301-4304	CD	denotes	5.5
T2696	4304-4305	-RRB-	denotes	)
T2697	4305-4306	.	denotes	.
T2698	4306-4399	sentence	denotes	Debris was sedimented by centrifugation, and 3 μl of supernatant was used for amplification.
T2699	4307-4313	NN	denotes	Debris
T2701	4314-4317	VBD	denotes	was
T2700	4318-4328	VBN	denotes	sedimented
T2702	4329-4331	IN	denotes	by
T2703	4332-4346	NN	denotes	centrifugation
T2704	4346-4348	,	denotes	,
T2705	4348-4351	CC	denotes	and
T2706	4352-4353	CD	denotes	3
T2707	4354-4356	NN	denotes	μl
T2709	4357-4359	IN	denotes	of
T2710	4360-4371	NN	denotes	supernatant
T2711	4372-4375	VBD	denotes	was
T2708	4376-4380	VBN	denotes	used
T2712	4381-4384	IN	denotes	for
T2713	4385-4398	NN	denotes	amplification
T2714	4398-4399	.	denotes	.
T2715	4399-4506	sentence	denotes	Genotyping for APP and tTA transgenes was performed in the same PCR reaction, using five separate primers.
T2716	4400-4410	VBG	denotes	Genotyping
T2718	4411-4414	IN	denotes	for
T2719	4415-4418	NN	denotes	APP
T2721	4419-4422	CC	denotes	and
T2722	4423-4426	NN	denotes	tTA
T2720	4427-4437	NNS	denotes	transgenes
T2723	4438-4441	VBD	denotes	was
T2717	4442-4451	VBN	denotes	performed
T2724	4452-4454	IN	denotes	in
T2725	4455-4458	DT	denotes	the
T2727	4459-4463	JJ	denotes	same
T2728	4464-4467	NN	denotes	PCR
T2726	4468-4476	NN	denotes	reaction
T2729	4476-4478	,	denotes	,
T2730	4478-4483	VBG	denotes	using
T2731	4484-4488	CD	denotes	five
T2733	4489-4497	JJ	denotes	separate
T2732	4498-4505	NNS	denotes	primers
T2734	4505-4506	.	denotes	.
T2735	4506-4723	sentence	denotes	APP was amplified using forward primer S36 located in the 3′ end of the APP cDNA (CCG AGA TCT CTG AAG TGA AGA TGG ATG) and reverse primer PrP-AS-J located in the 3′ UTR of the vector (CCA AGC CTA GAC CAC GAG AAT GC).
T2736	4507-4510	NN	denotes	APP
T2738	4511-4514	VBD	denotes	was
T2737	4515-4524	VBN	denotes	amplified
T2739	4525-4530	VBG	denotes	using
T2740	4531-4538	RB	denotes	forward
T2742	4539-4545	NN	denotes	primer
T2741	4546-4549	NN	denotes	S36
T2743	4550-4557	VBN	denotes	located
T2744	4558-4560	IN	denotes	in
T2745	4561-4564	DT	denotes	the
T2747	4565-4566	CD	denotes	3
T2748	4566-4567	SYM	denotes	′
T2746	4568-4571	NN	denotes	end
T2749	4572-4574	IN	denotes	of
T2750	4575-4578	DT	denotes	the
T2752	4579-4582	NN	denotes	APP
T2751	4583-4587	NN	denotes	cDNA
T2753	4588-4589	-LRB-	denotes	(
T2755	4589-4592	NN	denotes	CCG
T2756	4593-4596	NN	denotes	AGA
T2757	4597-4600	NN	denotes	TCT
T2758	4601-4604	NN	denotes	CTG
T2759	4605-4608	NN	denotes	AAG
T2760	4609-4612	NN	denotes	TGA
T2761	4613-4616	NN	denotes	AGA
T2762	4617-4620	NN	denotes	TGG
T2754	4621-4624	NN	denotes	ATG
T2763	4624-4625	-RRB-	denotes	)
T2764	4626-4629	CC	denotes	and
T2765	4630-4637	JJ	denotes	reverse
T2767	4638-4644	NN	denotes	primer
T2768	4645-4648	NN	denotes	PrP
T2769	4648-4649	HYPH	denotes	-
T2770	4649-4651	NN	denotes	AS
T2771	4651-4652	HYPH	denotes	-
T2766	4652-4653	NN	denotes	J
T2772	4654-4661	VBN	denotes	located
T2773	4662-4664	IN	denotes	in
T2774	4665-4668	DT	denotes	the
T2776	4669-4670	CD	denotes	3
T2777	4670-4671	SYM	denotes	′
T2775	4672-4675	NN	denotes	UTR
T2778	4676-4678	IN	denotes	of
T2779	4679-4682	DT	denotes	the
T2780	4683-4689	NN	denotes	vector
T2781	4690-4691	-LRB-	denotes	(
T2783	4691-4694	NN	denotes	CCA
T2784	4695-4698	NN	denotes	AGC
T2785	4699-4702	NN	denotes	CTA
T2786	4703-4706	NN	denotes	GAC
T2787	4707-4710	NN	denotes	CAC
T2788	4711-4714	NN	denotes	GAG
T2789	4715-4718	NN	denotes	AAT
T2782	4719-4721	NN	denotes	GC
T2790	4721-4722	-RRB-	denotes	)
T2791	4722-4723	.	denotes	.
T2792	4723-4979	sentence	denotes	The tTA transgene was detected using a primer set that amplified across its two subdomains with tet forward located within the Tn10 tetracycline repressor (CGC TGT GGG GCA TTT TAC TTT AG) and tet reverse within the HSV1 VP16 (CAT GTC CAG ATC GAA ATC GTC).
T2793	4724-4727	DT	denotes	The
T2795	4728-4731	NN	denotes	tTA
T2794	4732-4741	NN	denotes	transgene
T2797	4742-4745	VBD	denotes	was
T2796	4746-4754	VBN	denotes	detected
T2798	4755-4760	VBG	denotes	using
T2799	4761-4762	DT	denotes	a
T2801	4763-4769	NN	denotes	primer
T2800	4770-4773	NN	denotes	set
T2802	4774-4778	WDT	denotes	that
T2803	4779-4788	VBD	denotes	amplified
T2804	4789-4795	IN	denotes	across
T2805	4796-4799	PRP$	denotes	its
T2807	4800-4803	CD	denotes	two
T2806	4804-4814	NNS	denotes	subdomains
T2808	4815-4819	IN	denotes	with
T2809	4820-4823	NN	denotes	tet
T2810	4824-4831	RB	denotes	forward
T2811	4832-4839	VBN	denotes	located
T2812	4840-4846	IN	denotes	within
T2813	4847-4850	DT	denotes	the
T2815	4851-4855	NN	denotes	Tn10
T2816	4856-4868	NN	denotes	tetracycline
T2814	4869-4878	NN	denotes	repressor
T2817	4879-4880	-LRB-	denotes	(
T2819	4880-4883	NN	denotes	CGC
T2820	4884-4887	NN	denotes	TGT
T2821	4888-4891	NN	denotes	GGG
T2822	4892-4895	NN	denotes	GCA
T2823	4896-4899	NN	denotes	TTT
T2824	4900-4903	NN	denotes	TAC
T2825	4904-4907	NN	denotes	TTT
T2818	4908-4910	NN	denotes	AG
T2826	4910-4911	-RRB-	denotes	)
T2827	4912-4915	CC	denotes	and
T2828	4916-4919	NN	denotes	tet
T2829	4920-4927	JJ	denotes	reverse
T2830	4928-4934	IN	denotes	within
T2831	4935-4938	DT	denotes	the
T2833	4939-4943	NN	denotes	HSV1
T2832	4944-4948	NN	denotes	VP16
T2834	4949-4950	-LRB-	denotes	(
T2836	4950-4953	NN	denotes	CAT
T2837	4954-4957	NN	denotes	GTC
T2838	4958-4961	NN	denotes	CAG
T2839	4962-4965	NN	denotes	ATC
T2840	4966-4969	NN	denotes	GAA
T2841	4970-4973	NN	denotes	ATC
T2835	4974-4977	NN	denotes	GTC
T2842	4977-4978	-RRB-	denotes	)
T2843	4978-4979	.	denotes	.
T2844	4979-5332	sentence	denotes	All reactions, whether transgene-positive or not, amplified a segment of the endogenous prion protein gene as a control for DNA quality using a forward primer, PrP-S-J, specific to the mouse PrP open reading frame (GGG ACT ATG TGG ACT GAT GTC GG) and a reverse primer, PrP-AS-J, shared by the 3′ UTR of the endogenous PrP gene and the transgene vector.
T2845	4980-4983	DT	denotes	All
T2846	4984-4993	NNS	denotes	reactions
T2848	4993-4995	,	denotes	,
T2849	4995-5002	IN	denotes	whether
T2851	5003-5012	NN	denotes	transgene
T2852	5012-5013	HYPH	denotes	-
T2850	5013-5021	JJ	denotes	positive
T2853	5022-5024	CC	denotes	or
T2854	5025-5028	RB	denotes	not
T2855	5028-5030	,	denotes	,
T2847	5030-5039	VBD	denotes	amplified
T2856	5040-5041	DT	denotes	a
T2857	5042-5049	NN	denotes	segment
T2858	5050-5052	IN	denotes	of
T2859	5053-5056	DT	denotes	the
T2861	5057-5067	JJ	denotes	endogenous
T2862	5068-5073	NN	denotes	prion
T2863	5074-5081	NN	denotes	protein
T2860	5082-5086	NN	denotes	gene
T2864	5087-5089	IN	denotes	as
T2865	5090-5091	DT	denotes	a
T2866	5092-5099	NN	denotes	control
T2867	5100-5103	IN	denotes	for
T2868	5104-5107	NN	denotes	DNA
T2869	5108-5115	NN	denotes	quality
T2870	5116-5121	VBG	denotes	using
T2871	5122-5123	DT	denotes	a
T2873	5124-5131	JJ	denotes	forward
T2872	5132-5138	NN	denotes	primer
T2874	5138-5140	,	denotes	,
T2875	5140-5143	NN	denotes	PrP
T2877	5143-5144	HYPH	denotes	-
T2878	5144-5145	NN	denotes	S
T2879	5145-5146	HYPH	denotes	-
T2876	5146-5147	NN	denotes	J
T2880	5147-5149	,	denotes	,
T2881	5149-5157	JJ	denotes	specific
T2882	5158-5160	IN	denotes	to
T2883	5161-5164	DT	denotes	the
T2885	5165-5170	NN	denotes	mouse
T2886	5171-5174	NN	denotes	PrP
T2887	5175-5179	JJ	denotes	open
T2888	5180-5187	NN	denotes	reading
T2884	5188-5193	NN	denotes	frame
T2889	5194-5195	-LRB-	denotes	(
T2891	5195-5198	NN	denotes	GGG
T2892	5199-5202	NN	denotes	ACT
T2893	5203-5206	NN	denotes	ATG
T2894	5207-5210	NN	denotes	TGG
T2895	5211-5214	NN	denotes	ACT
T2896	5215-5218	NN	denotes	GAT
T2897	5219-5222	NN	denotes	GTC
T2890	5223-5225	NN	denotes	GG
T2898	5225-5226	-RRB-	denotes	)
T2899	5227-5230	CC	denotes	and
T2900	5231-5232	DT	denotes	a
T2902	5233-5240	JJ	denotes	reverse
T2901	5241-5247	NN	denotes	primer
T2903	5247-5249	,	denotes	,
T2904	5249-5252	NN	denotes	PrP
T2906	5252-5253	HYPH	denotes	-
T2907	5253-5255	NN	denotes	AS
T2908	5255-5256	HYPH	denotes	-
T2905	5256-5257	NN	denotes	J
T2909	5257-5259	,	denotes	,
T2910	5259-5265	VBN	denotes	shared
T2911	5266-5268	IN	denotes	by
T2912	5269-5272	DT	denotes	the
T2914	5273-5274	CD	denotes	3
T2915	5274-5275	SYM	denotes	′
T2913	5276-5279	NN	denotes	UTR
T2916	5280-5282	IN	denotes	of
T2917	5283-5286	DT	denotes	the
T2919	5287-5297	JJ	denotes	endogenous
T2920	5298-5301	NN	denotes	PrP
T2918	5302-5306	NN	denotes	gene
T2921	5307-5310	CC	denotes	and
T2922	5311-5314	DT	denotes	the
T2924	5315-5324	NN	denotes	transgene
T2923	5325-5331	NN	denotes	vector
T2925	5331-5332	.	denotes	.
T2926	5332-5436	sentence	denotes	Amplification reactions were run for 37 cycles at 94 °C for 30 s, 64 °C for 1 min, and 72 °C for 1 min.
T2927	5333-5346	NN	denotes	Amplification
T2928	5347-5356	NNS	denotes	reactions
T2930	5357-5361	VBD	denotes	were
T2929	5362-5365	VBN	denotes	run
T2931	5366-5369	IN	denotes	for
T2932	5370-5372	CD	denotes	37
T2933	5373-5379	NNS	denotes	cycles
T2934	5380-5382	IN	denotes	at
T2935	5383-5385	CD	denotes	94
T2936	5386-5388	NN	denotes	°C
T2937	5389-5392	IN	denotes	for
T2938	5393-5395	CD	denotes	30
T2939	5396-5397	NN	denotes	s
T2940	5397-5399	,	denotes	,
T2941	5399-5401	CD	denotes	64
T2942	5402-5404	NN	denotes	°C
T2943	5405-5408	IN	denotes	for
T2944	5409-5410	CD	denotes	1
T2945	5411-5414	NN	denotes	min
T2946	5414-5416	,	denotes	,
T2947	5416-5419	CC	denotes	and
T2948	5420-5422	CD	denotes	72
T2949	5423-5425	NN	denotes	°C
T2950	5426-5429	IN	denotes	for
T2951	5430-5431	CD	denotes	1
T2952	5432-5435	NN	denotes	min
T2953	5435-5436	.	denotes	.
T2954	5436-5527	sentence	denotes	All samples, transgenic and wild-type, gave a 750-bp product from the endogenous PrP gene.
T2955	5437-5440	DT	denotes	All
T2956	5441-5448	NNS	denotes	samples
T2958	5448-5450	,	denotes	,
T2959	5450-5460	JJ	denotes	transgenic
T2960	5461-5464	CC	denotes	and
T2961	5465-5469	JJ	denotes	wild
T2963	5469-5470	HYPH	denotes	-
T2962	5470-5474	NN	denotes	type
T2964	5474-5476	,	denotes	,
T2957	5476-5480	VBD	denotes	gave
T2965	5481-5482	DT	denotes	a
T2967	5483-5486	CD	denotes	750
T2969	5486-5487	HYPH	denotes	-
T2968	5487-5489	NN	denotes	bp
T2966	5490-5497	NN	denotes	product
T2970	5498-5502	IN	denotes	from
T2971	5503-5506	DT	denotes	the
T2973	5507-5517	JJ	denotes	endogenous
T2974	5518-5521	NN	denotes	PrP
T2972	5522-5526	NN	denotes	gene
T2975	5526-5527	.	denotes	.
T2976	5527-5626	sentence	denotes	The APP transgene yielded an additional band at 400 bp; the tTA product fell in between at 480 bp.
T2977	5528-5531	DT	denotes	The
T2979	5532-5535	NN	denotes	APP
T2978	5536-5545	NN	denotes	transgene
T2980	5546-5553	VBD	denotes	yielded
T2982	5554-5556	DT	denotes	an
T2984	5557-5567	JJ	denotes	additional
T2983	5568-5572	NN	denotes	band
T2985	5573-5575	IN	denotes	at
T2986	5576-5579	CD	denotes	400
T2987	5580-5582	NN	denotes	bp
T2988	5582-5583	:	denotes	;
T2989	5584-5587	DT	denotes	the
T2991	5588-5591	NN	denotes	tTA
T2990	5592-5599	NN	denotes	product
T2981	5600-5604	VBD	denotes	fell
T2992	5605-5607	RB	denotes	in
T2993	5608-5615	RB	denotes	between
T2994	5616-5618	IN	denotes	at
T2995	5619-5622	CD	denotes	480
T2996	5623-5625	NN	denotes	bp
T2997	5625-5626	.	denotes	.
T3141	5628-5642	NN	denotes	Immunoblotting
T3143	5642-5643	HYPH	denotes	/
T3142	5643-5655	NN	denotes	Quantitation
T3144	5655-5972	sentence	denotes	Frozen cortical or whole forebrain tissue was homogenized by sonication in five volumes of phosphate-buffered saline (PBS) with 5 mM EDTA and protease inhibitors (Mammalian cell cocktail, Sigma, St. Louis, Missouri, United States), using a probe sonicator set to 50% output (TEKMAR, Cincinnati, Ohio, United States).
T3145	5656-5662	VBN	denotes	Frozen
T3147	5663-5671	JJ	denotes	cortical
T3148	5672-5674	CC	denotes	or
T3149	5675-5680	JJ	denotes	whole
T3150	5681-5690	NN	denotes	forebrain
T3146	5691-5697	NN	denotes	tissue
T3152	5698-5701	VBD	denotes	was
T3151	5702-5713	VBN	denotes	homogenized
T3153	5714-5716	IN	denotes	by
T3154	5717-5727	NN	denotes	sonication
T3155	5728-5730	IN	denotes	in
T3156	5731-5735	CD	denotes	five
T3157	5736-5743	NNS	denotes	volumes
T3158	5744-5746	IN	denotes	of
T3159	5747-5756	NN	denotes	phosphate
T3161	5756-5757	HYPH	denotes	-
T3160	5757-5765	VBN	denotes	buffered
T3162	5766-5772	NN	denotes	saline
T3163	5773-5774	-LRB-	denotes	(
T3164	5774-5777	NN	denotes	PBS
T3165	5777-5778	-RRB-	denotes	)
T3166	5779-5783	IN	denotes	with
T3167	5784-5785	CD	denotes	5
T3168	5786-5788	NN	denotes	mM
T3170	5789-5793	NN	denotes	EDTA
T3171	5794-5797	CC	denotes	and
T3172	5798-5806	NN	denotes	protease
T3169	5807-5817	NNS	denotes	inhibitors
T3173	5818-5819	-LRB-	denotes	(
T3175	5819-5828	JJ	denotes	Mammalian
T3176	5829-5833	NN	denotes	cell
T3174	5834-5842	NN	denotes	cocktail
T3177	5842-5844	,	denotes	,
T3178	5844-5849	NNP	denotes	Sigma
T3179	5849-5851	,	denotes	,
T3180	5851-5854	NNP	denotes	St.
T3181	5855-5860	NNP	denotes	Louis
T3182	5860-5862	,	denotes	,
T3183	5862-5870	NNP	denotes	Missouri
T3184	5870-5872	,	denotes	,
T3185	5872-5878	NNP	denotes	United
T3186	5879-5885	NNP	denotes	States
T3187	5885-5886	-RRB-	denotes	)
T3188	5886-5888	,	denotes	,
T3189	5888-5893	VBG	denotes	using
T3190	5894-5895	DT	denotes	a
T3192	5896-5901	NN	denotes	probe
T3191	5902-5911	NN	denotes	sonicator
T3193	5912-5915	VBN	denotes	set
T3194	5916-5918	IN	denotes	to
T3195	5919-5921	CD	denotes	50
T3196	5921-5922	NN	denotes	%
T3197	5923-5929	NN	denotes	output
T3198	5930-5931	-LRB-	denotes	(
T3199	5931-5937	NNP	denotes	TEKMAR
T3200	5937-5939	,	denotes	,
T3201	5939-5949	NNP	denotes	Cincinnati
T3202	5949-5951	,	denotes	,
T3203	5951-5955	NNP	denotes	Ohio
T3204	5955-5957	,	denotes	,
T3205	5957-5963	NNP	denotes	United
T3206	5964-5970	NNP	denotes	States
T3207	5970-5971	-RRB-	denotes	)
T3208	5971-5972	.	denotes	.
T3209	5972-6116	sentence	denotes	After dilution with an equal volume of PBS/EDTA/protease inhibitor, the samples were centrifuged briefly and the supernatant used for analysis.
T3210	5973-5978	IN	denotes	After
T3212	5979-5987	NN	denotes	dilution
T3213	5988-5992	IN	denotes	with
T3214	5993-5995	DT	denotes	an
T3216	5996-6001	JJ	denotes	equal
T3215	6002-6008	NN	denotes	volume
T3217	6009-6011	IN	denotes	of
T3218	6012-6015	NN	denotes	PBS
T3220	6015-6016	HYPH	denotes	/
T3221	6016-6020	NN	denotes	EDTA
T3222	6020-6021	HYPH	denotes	/
T3219	6021-6029	NN	denotes	protease
T3223	6030-6039	NN	denotes	inhibitor
T3224	6039-6041	,	denotes	,
T3225	6041-6044	DT	denotes	the
T3226	6045-6052	NNS	denotes	samples
T3227	6053-6057	VBD	denotes	were
T3211	6058-6069	VBN	denotes	centrifuged
T3228	6070-6077	RB	denotes	briefly
T3229	6078-6081	CC	denotes	and
T3230	6082-6085	DT	denotes	the
T3231	6086-6097	NN	denotes	supernatant
T3232	6098-6102	VBN	denotes	used
T3233	6103-6106	IN	denotes	for
T3234	6107-6115	NN	denotes	analysis
T3235	6115-6116	.	denotes	.
T3236	6116-6553	sentence	denotes	Fifty micrograms (6E10 and CT15) or 5 μg (22C11) of brain homogenate was loaded per lane onto 7.5%, 10%–20%, or 4%–20% Tris-HCl PAGE gels (Bio-Rad Laboratories, Hercules, California, United States) and electrophoresed for several hours in 1× Tris-glycine–sodium dodecyl sulfate (1×TG-SDS) buffer (6E10 and 22C11; Amresco, Solon, Ohio, United States) or 1× Tris-tricine-SDS buffer (CT15; Invitrogen, Carlsbad, California, United States).
T3237	6117-6122	CD	denotes	Fifty
T3238	6123-6133	NNS	denotes	micrograms
T3240	6134-6135	-LRB-	denotes	(
T3239	6135-6139	NN	denotes	6E10
T3242	6140-6143	CC	denotes	and
T3243	6144-6148	NN	denotes	CT15
T3244	6148-6149	-RRB-	denotes	)
T3245	6150-6152	CC	denotes	or
T3246	6153-6154	CD	denotes	5
T3247	6155-6157	NN	denotes	μg
T3249	6158-6159	-LRB-	denotes	(
T3248	6159-6164	NN	denotes	22C11
T3250	6164-6165	-RRB-	denotes	)
T3251	6166-6168	IN	denotes	of
T3252	6169-6174	NN	denotes	brain
T3253	6175-6185	NN	denotes	homogenate
T3254	6186-6189	VBD	denotes	was
T3241	6190-6196	VBN	denotes	loaded
T3255	6197-6200	IN	denotes	per
T3256	6201-6205	NN	denotes	lane
T3257	6206-6210	IN	denotes	onto
T3258	6211-6214	CD	denotes	7.5
T3259	6214-6215	NN	denotes	%
T3261	6215-6217	,	denotes	,
T3262	6217-6219	CD	denotes	10
T3263	6219-6220	NN	denotes	%
T3264	6220-6221	SYM	denotes	–
T3266	6221-6223	CD	denotes	20
T3265	6223-6224	NN	denotes	%
T3267	6224-6226	,	denotes	,
T3268	6226-6228	CC	denotes	or
T3269	6229-6230	CD	denotes	4
T3270	6230-6231	NN	denotes	%
T3271	6231-6232	SYM	denotes	–
T3273	6232-6234	CD	denotes	20
T3272	6234-6235	NN	denotes	%
T3274	6236-6240	NN	denotes	Tris
T3275	6240-6241	HYPH	denotes	-
T3276	6241-6244	NN	denotes	HCl
T3260	6245-6249	NN	denotes	PAGE
T3277	6250-6254	NNS	denotes	gels
T3278	6255-6256	-LRB-	denotes	(
T3280	6256-6259	NNP	denotes	Bio
T3282	6259-6260	HYPH	denotes	-
T3281	6260-6263	NNP	denotes	Rad
T3279	6264-6276	NNP	denotes	Laboratories
T3283	6276-6278	,	denotes	,
T3284	6278-6286	NNP	denotes	Hercules
T3285	6286-6288	,	denotes	,
T3286	6288-6298	NNP	denotes	California
T3287	6298-6300	,	denotes	,
T3288	6300-6306	NNP	denotes	United
T3289	6307-6313	NNP	denotes	States
T3290	6313-6314	-RRB-	denotes	)
T3291	6315-6318	CC	denotes	and
T3292	6319-6334	VBN	denotes	electrophoresed
T3293	6335-6338	IN	denotes	for
T3294	6339-6346	JJ	denotes	several
T3295	6347-6352	NNS	denotes	hours
T3296	6353-6355	IN	denotes	in
T3297	6356-6357	CD	denotes	1
T3299	6357-6358	SYM	denotes	×
T3300	6359-6363	NN	denotes	Tris
T3301	6363-6364	HYPH	denotes	-
T3302	6364-6371	NN	denotes	glycine
T3303	6371-6372	HYPH	denotes	–
T3304	6372-6378	NN	denotes	sodium
T3305	6379-6386	NN	denotes	dodecyl
T3298	6387-6394	NN	denotes	sulfate
T3307	6395-6396	-LRB-	denotes	(
T3308	6396-6397	CD	denotes	1
T3310	6397-6398	SYM	denotes	×
T3311	6398-6400	NN	denotes	TG
T3312	6400-6401	HYPH	denotes	-
T3309	6401-6404	NN	denotes	SDS
T3313	6404-6405	-RRB-	denotes	)
T3306	6406-6412	NN	denotes	buffer
T3314	6413-6414	-LRB-	denotes	(
T3316	6414-6418	NN	denotes	6E10
T3317	6419-6422	CC	denotes	and
T3318	6423-6428	NN	denotes	22C11
T3319	6428-6429	:	denotes	;
T3315	6430-6437	NNP	denotes	Amresco
T3320	6437-6439	,	denotes	,
T3321	6439-6444	NNP	denotes	Solon
T3322	6444-6446	,	denotes	,
T3323	6446-6450	NNP	denotes	Ohio
T3324	6450-6452	,	denotes	,
T3325	6452-6458	NNP	denotes	United
T3326	6459-6465	NNP	denotes	States
T3327	6465-6466	-RRB-	denotes	)
T3328	6467-6469	CC	denotes	or
T3329	6470-6471	CD	denotes	1
T3331	6471-6472	SYM	denotes	×
T3332	6473-6477	NN	denotes	Tris
T3334	6477-6478	HYPH	denotes	-
T3335	6478-6485	NN	denotes	tricine
T3336	6485-6486	HYPH	denotes	-
T3333	6486-6489	NN	denotes	SDS
T3330	6490-6496	NN	denotes	buffer
T3337	6497-6498	-LRB-	denotes	(
T3339	6498-6502	NN	denotes	CT15
T3340	6502-6503	:	denotes	;
T3338	6504-6514	NNP	denotes	Invitrogen
T3341	6514-6516	,	denotes	,
T3342	6516-6524	NNP	denotes	Carlsbad
T3343	6524-6526	,	denotes	,
T3344	6526-6536	NNP	denotes	California
T3345	6536-6538	,	denotes	,
T3346	6538-6544	NNP	denotes	United
T3347	6545-6551	NNP	denotes	States
T3348	6551-6552	-RRB-	denotes	)
T3349	6552-6553	.	denotes	.
T3350	6553-6713	sentence	denotes	Proteins were transferred overnight to 0.45-μm Optitran nitrocellulose (Schleicher and Schuell, Keene, New Hampshire, United States) in 1× TG buffer (Amresco).
T3351	6554-6562	NNS	denotes	Proteins
T3353	6563-6567	VBD	denotes	were
T3352	6568-6579	VBN	denotes	transferred
T3354	6580-6589	RB	denotes	overnight
T3355	6590-6592	IN	denotes	to
T3356	6593-6597	CD	denotes	0.45
T3358	6597-6598	HYPH	denotes	-
T3357	6598-6600	NN	denotes	μm
T3360	6601-6609	NNP	denotes	Optitran
T3359	6610-6624	NN	denotes	nitrocellulose
T3361	6625-6626	-LRB-	denotes	(
T3362	6626-6636	NNP	denotes	Schleicher
T3363	6637-6640	CC	denotes	and
T3364	6641-6648	NNP	denotes	Schuell
T3365	6648-6650	,	denotes	,
T3366	6650-6655	NNP	denotes	Keene
T3367	6655-6657	,	denotes	,
T3368	6657-6660	NNP	denotes	New
T3369	6661-6670	NNP	denotes	Hampshire
T3370	6670-6672	,	denotes	,
T3371	6672-6678	NNP	denotes	United
T3372	6679-6685	NNP	denotes	States
T3373	6685-6686	-RRB-	denotes	)
T3374	6687-6689	IN	denotes	in
T3375	6690-6691	CD	denotes	1
T3377	6691-6692	SYM	denotes	×
T3378	6693-6695	NN	denotes	TG
T3376	6696-6702	NN	denotes	buffer
T3379	6703-6704	-LRB-	denotes	(
T3380	6704-6711	NNP	denotes	Amresco
T3381	6711-6712	-RRB-	denotes	)
T3382	6712-6713	.	denotes	.
T3383	6713-7238	sentence	denotes	Blots were blocked in PBS containing 5% nonfat dry milk powder, and incubated for 3 h at room temperature in blocking solution with one of the following antibodies: mouse monoclonal 22C11 (kind gift of Konrad Beyreuther and Andreas Weidemann; [17]) diluted 1:1,000, mouse monoclonal 6E10 (Signet Laboratories, Dedham, Massachusetts, United States) diluted 1:2,500, rabbit polyclonal anti-superoxide dismutase 1 (m/hSOD1) [18] diluted 1:2,500 to 1:4,000, or rabbit polyclonal CT15 (kind gift of Ed Koo; [19]) diluted 1:1,000.
T3384	6714-6719	NNS	denotes	Blots
T3386	6720-6724	VBD	denotes	were
T3385	6725-6732	VBN	denotes	blocked
T3387	6733-6735	IN	denotes	in
T3388	6736-6739	NN	denotes	PBS
T3389	6740-6750	VBG	denotes	containing
T3390	6751-6752	CD	denotes	5
T3391	6752-6753	NN	denotes	%
T3393	6754-6760	JJ	denotes	nonfat
T3395	6761-6764	JJ	denotes	dry
T3394	6765-6769	NN	denotes	milk
T3392	6770-6776	NN	denotes	powder
T3396	6776-6778	,	denotes	,
T3397	6778-6781	CC	denotes	and
T3398	6782-6791	VBN	denotes	incubated
T3399	6792-6795	IN	denotes	for
T3400	6796-6797	CD	denotes	3
T3401	6798-6799	NN	denotes	h
T3402	6800-6802	IN	denotes	at
T3403	6803-6807	NN	denotes	room
T3404	6808-6819	NN	denotes	temperature
T3405	6820-6822	IN	denotes	in
T3406	6823-6831	NN	denotes	blocking
T3407	6832-6840	NN	denotes	solution
T3408	6841-6845	IN	denotes	with
T3409	6846-6849	CD	denotes	one
T3410	6850-6852	IN	denotes	of
T3411	6853-6856	DT	denotes	the
T3413	6857-6866	VBG	denotes	following
T3412	6867-6877	NNS	denotes	antibodies
T3414	6877-6879	:	denotes	:
T3415	6879-6884	NN	denotes	mouse
T3417	6885-6895	JJ	denotes	monoclonal
T3416	6896-6901	NN	denotes	22C11
T3418	6902-6903	-LRB-	denotes	(
T3420	6903-6907	JJ	denotes	kind
T3421	6908-6912	NN	denotes	gift
T3422	6913-6915	IN	denotes	of
T3423	6916-6922	NNP	denotes	Konrad
T3424	6923-6933	NNP	denotes	Beyreuther
T3425	6934-6937	CC	denotes	and
T3426	6938-6945	NNP	denotes	Andreas
T3427	6946-6955	NNP	denotes	Weidemann
T3428	6955-6956	:	denotes	;
T3429	6957-6958	-LRB-	denotes	[
T3419	6958-6960	CD	denotes	17
T3430	6960-6961	-RRB-	denotes	]
T3431	6961-6962	-RRB-	denotes	)
T3432	6963-6970	VBN	denotes	diluted
T3433	6971-6972	CD	denotes	1
T3434	6972-6973	SYM	denotes	:
T3435	6973-6978	CD	denotes	1,000
T3436	6978-6980	,	denotes	,
T3437	6980-6985	NN	denotes	mouse
T3439	6986-6996	JJ	denotes	monoclonal
T3438	6997-7001	NN	denotes	6E10
T3440	7002-7003	-LRB-	denotes	(
T3442	7003-7009	NNP	denotes	Signet
T3441	7010-7022	NNP	denotes	Laboratories
T3443	7022-7024	,	denotes	,
T3444	7024-7030	NNP	denotes	Dedham
T3445	7030-7032	,	denotes	,
T3446	7032-7045	NNP	denotes	Massachusetts
T3447	7045-7047	,	denotes	,
T3448	7047-7053	NNP	denotes	United
T3449	7054-7060	NNP	denotes	States
T3450	7060-7061	-RRB-	denotes	)
T3451	7062-7069	VBN	denotes	diluted
T3452	7070-7071	CD	denotes	1
T3453	7071-7072	SYM	denotes	:
T3454	7072-7077	CD	denotes	2,500
T3455	7077-7079	,	denotes	,
T3456	7079-7085	NN	denotes	rabbit
T3458	7086-7096	JJ	denotes	polyclonal
T3459	7097-7112	JJ	denotes	anti-superoxide
T3460	7113-7122	NN	denotes	dismutase
T3461	7123-7124	CD	denotes	1
T3462	7125-7126	-LRB-	denotes	(
T3463	7126-7127	NN	denotes	m
T3464	7127-7128	HYPH	denotes	/
T3457	7128-7133	NN	denotes	hSOD1
T3465	7133-7134	-RRB-	denotes	)
T3466	7135-7136	-LRB-	denotes	[
T3467	7136-7138	CD	denotes	18
T3468	7138-7139	-RRB-	denotes	]
T3469	7140-7147	VBN	denotes	diluted
T3470	7148-7149	CD	denotes	1
T3471	7149-7150	SYM	denotes	:
T3472	7150-7155	CD	denotes	2,500
T3473	7156-7158	IN	denotes	to
T3474	7159-7160	CD	denotes	1
T3475	7160-7161	SYM	denotes	:
T3476	7161-7166	CD	denotes	4,000
T3477	7166-7168	,	denotes	,
T3478	7168-7170	CC	denotes	or
T3479	7171-7177	NN	denotes	rabbit
T3481	7178-7188	JJ	denotes	polyclonal
T3480	7189-7193	NN	denotes	CT15
T3482	7194-7195	-LRB-	denotes	(
T3484	7195-7199	JJ	denotes	kind
T3483	7200-7204	NN	denotes	gift
T3485	7205-7207	IN	denotes	of
T3486	7208-7210	NNP	denotes	Ed
T3487	7211-7214	NNP	denotes	Koo
T3488	7214-7215	:	denotes	;
T3489	7216-7217	-LRB-	denotes	[
T3490	7217-7219	CD	denotes	19
T3491	7219-7220	-RRB-	denotes	]
T3492	7220-7221	-RRB-	denotes	)
T3493	7222-7229	VBN	denotes	diluted
T3494	7230-7231	CD	denotes	1
T3495	7231-7232	SYM	denotes	:
T3496	7232-7237	CD	denotes	1,000
T3497	7237-7238	.	denotes	.
T3498	7238-7449	sentence	denotes	Subsequently, the blots were washed with PBS containing 0.1% Tween-20, and then incubated with either goat anti-mouse– or goat anti-rabbit–HRP conjugated secondary antibody diluted 1:1,000 in blocking solution.
T3499	7239-7251	RB	denotes	Subsequently
T3501	7251-7253	,	denotes	,
T3502	7253-7256	DT	denotes	the
T3503	7257-7262	NNS	denotes	blots
T3504	7263-7267	VBD	denotes	were
T3500	7268-7274	VBN	denotes	washed
T3505	7275-7279	IN	denotes	with
T3506	7280-7283	NN	denotes	PBS
T3507	7284-7294	VBG	denotes	containing
T3508	7295-7298	CD	denotes	0.1
T3509	7298-7299	NN	denotes	%
T3510	7300-7305	NN	denotes	Tween
T3511	7305-7306	HYPH	denotes	-
T3512	7306-7308	CD	denotes	20
T3513	7308-7310	,	denotes	,
T3514	7310-7313	CC	denotes	and
T3515	7314-7318	RB	denotes	then
T3516	7319-7328	VBN	denotes	incubated
T3517	7329-7333	IN	denotes	with
T3518	7334-7340	CC	denotes	either
T3519	7341-7345	NN	denotes	goat
T3521	7346-7356	JJ	denotes	anti-mouse
T3522	7356-7357	HYPH	denotes	–
T3523	7358-7360	CC	denotes	or
T3524	7361-7365	NN	denotes	goat
T3525	7366-7377	JJ	denotes	anti-rabbit
T3526	7377-7378	HYPH	denotes	–
T3527	7378-7381	NN	denotes	HRP
T3528	7382-7392	VBN	denotes	conjugated
T3529	7393-7402	JJ	denotes	secondary
T3520	7403-7411	NN	denotes	antibody
T3530	7412-7419	VBN	denotes	diluted
T3531	7420-7421	CD	denotes	1
T3532	7421-7422	SYM	denotes	:
T3533	7422-7427	CD	denotes	1,000
T3534	7428-7430	IN	denotes	in
T3535	7431-7439	NN	denotes	blocking
T3536	7440-7448	NN	denotes	solution
T3537	7448-7449	.	denotes	.
T3538	7449-7625	sentence	denotes	After several additional rinses in PBS with 0.1% Tween-20, blots were developed with enhanced chemiluminescence reagent and imaged with the Bio-Rad Molecular Imager FX system.
T3539	7450-7455	IN	denotes	After
T3541	7456-7463	JJ	denotes	several
T3543	7464-7474	JJ	denotes	additional
T3542	7475-7481	NNS	denotes	rinses
T3544	7482-7484	IN	denotes	in
T3545	7485-7488	NN	denotes	PBS
T3546	7489-7493	IN	denotes	with
T3547	7494-7497	CD	denotes	0.1
T3549	7497-7498	NN	denotes	%
T3548	7499-7504	NN	denotes	Tween
T3550	7504-7505	HYPH	denotes	-
T3551	7505-7507	CD	denotes	20
T3552	7507-7509	,	denotes	,
T3553	7509-7514	NNS	denotes	blots
T3554	7515-7519	VBD	denotes	were
T3540	7520-7529	VBN	denotes	developed
T3555	7530-7534	IN	denotes	with
T3556	7535-7543	VBN	denotes	enhanced
T3558	7544-7561	NN	denotes	chemiluminescence
T3557	7562-7569	NN	denotes	reagent
T3559	7570-7573	CC	denotes	and
T3560	7574-7580	VBN	denotes	imaged
T3561	7581-7585	IN	denotes	with
T3562	7586-7589	DT	denotes	the
T3564	7590-7593	NNP	denotes	Bio
T3566	7593-7594	HYPH	denotes	-
T3565	7594-7597	NNP	denotes	Rad
T3568	7598-7607	NNP	denotes	Molecular
T3569	7608-7614	NNP	denotes	Imager
T3567	7615-7617	NNP	denotes	FX
T3563	7618-7624	NN	denotes	system
T3570	7624-7625	.	denotes	.
T3571	7625-7768	sentence	denotes	Staining intensity within each lane was quantified using the Quantity One image analysis software (Molecular Imager FX, Bio-Rad Laboratories).
T3572	7626-7634	NN	denotes	Staining
T3573	7635-7644	NN	denotes	intensity
T3575	7645-7651	IN	denotes	within
T3576	7652-7656	DT	denotes	each
T3577	7657-7661	NN	denotes	lane
T3578	7662-7665	VBD	denotes	was
T3574	7666-7676	VBN	denotes	quantified
T3579	7677-7682	VBG	denotes	using
T3580	7683-7686	DT	denotes	the
T3582	7687-7695	NNP	denotes	Quantity
T3583	7696-7699	CD	denotes	One
T3584	7700-7705	NN	denotes	image
T3585	7706-7714	NN	denotes	analysis
T3581	7715-7723	NN	denotes	software
T3586	7724-7725	-LRB-	denotes	(
T3588	7725-7734	NNP	denotes	Molecular
T3589	7735-7741	NNP	denotes	Imager
T3587	7742-7744	NNP	denotes	FX
T3590	7744-7746	,	denotes	,
T3591	7746-7749	NNP	denotes	Bio
T3593	7749-7750	HYPH	denotes	-
T3592	7750-7753	NNP	denotes	Rad
T3594	7754-7766	NNP	denotes	Laboratories
T3595	7766-7767	-RRB-	denotes	)
T3596	7767-7768	.	denotes	.
T3597	7768-7853	sentence	denotes	Background was calculated from across the image and subtracted from the entire file.
T3598	7769-7779	NN	denotes	Background
T3600	7780-7783	VBD	denotes	was
T3599	7784-7794	VBN	denotes	calculated
T3601	7795-7799	IN	denotes	from
T3602	7800-7806	IN	denotes	across
T3603	7807-7810	DT	denotes	the
T3604	7811-7816	NN	denotes	image
T3605	7817-7820	CC	denotes	and
T3606	7821-7831	VBN	denotes	subtracted
T3607	7832-7836	IN	denotes	from
T3608	7837-7840	DT	denotes	the
T3610	7841-7847	JJ	denotes	entire
T3609	7848-7852	NN	denotes	file
T3611	7852-7853	.	denotes	.
T3612	7853-7982	sentence	denotes	The signal intensity for each band (corrected signal intensity × pixel number) was then calculated using the Volume report tool.
T3613	7854-7857	DT	denotes	The
T3615	7858-7864	NN	denotes	signal
T3614	7865-7874	NN	denotes	intensity
T3617	7875-7878	IN	denotes	for
T3618	7879-7883	DT	denotes	each
T3619	7884-7888	NN	denotes	band
T3620	7889-7890	-LRB-	denotes	(
T3622	7890-7899	VBN	denotes	corrected
T3623	7900-7906	NN	denotes	signal
T3621	7907-7916	NN	denotes	intensity
T3624	7917-7918	SYM	denotes	×
T3625	7919-7924	NN	denotes	pixel
T3626	7925-7931	NN	denotes	number
T3627	7931-7932	-RRB-	denotes	)
T3628	7933-7936	VBD	denotes	was
T3629	7937-7941	RB	denotes	then
T3616	7942-7952	VBN	denotes	calculated
T3630	7953-7958	VBG	denotes	using
T3631	7959-7962	DT	denotes	the
T3633	7963-7969	NN	denotes	Volume
T3634	7970-7976	NN	denotes	report
T3632	7977-7981	NN	denotes	tool
T3635	7981-7982	.	denotes	.
T3710	7984-7988	NN	denotes	Slot
T3711	7989-7993	NN	denotes	Blot
T3713	7994-7998	NN	denotes	mRNA
T3712	7999-8007	NN	denotes	Analysis
T3714	8007-8199	sentence	denotes	Five micrograms per sample of total RNA extracted from fresh-frozen brain, liver, kidney, heart, lung, spleen, and skeletal muscle was vacuum-filtered through 0.45-μm Optitran nitrocellulose.
T3715	8008-8012	CD	denotes	Five
T3716	8013-8023	NNS	denotes	micrograms
T3718	8024-8027	IN	denotes	per
T3719	8028-8034	NN	denotes	sample
T3720	8035-8037	IN	denotes	of
T3721	8038-8043	JJ	denotes	total
T3722	8044-8047	NN	denotes	RNA
T3723	8048-8057	VBN	denotes	extracted
T3724	8058-8062	IN	denotes	from
T3725	8063-8068	JJ	denotes	fresh
T3727	8068-8069	HYPH	denotes	-
T3726	8069-8075	VBN	denotes	frozen
T3728	8076-8081	NN	denotes	brain
T3729	8081-8083	,	denotes	,
T3730	8083-8088	NN	denotes	liver
T3731	8088-8090	,	denotes	,
T3732	8090-8096	NN	denotes	kidney
T3733	8096-8098	,	denotes	,
T3734	8098-8103	NN	denotes	heart
T3735	8103-8105	,	denotes	,
T3736	8105-8109	NN	denotes	lung
T3737	8109-8111	,	denotes	,
T3738	8111-8117	NN	denotes	spleen
T3739	8117-8119	,	denotes	,
T3740	8119-8122	CC	denotes	and
T3741	8123-8131	JJ	denotes	skeletal
T3742	8132-8138	NN	denotes	muscle
T3743	8139-8142	VBD	denotes	was
T3744	8143-8149	NN	denotes	vacuum
T3745	8149-8150	HYPH	denotes	-
T3717	8150-8158	VBN	denotes	filtered
T3746	8159-8166	IN	denotes	through
T3747	8167-8171	CD	denotes	0.45
T3749	8171-8172	HYPH	denotes	-
T3748	8172-8174	NN	denotes	μm
T3751	8175-8183	NNP	denotes	Optitran
T3750	8184-8198	NN	denotes	nitrocellulose
T3752	8198-8199	.	denotes	.
T3753	8199-8367	sentence	denotes	After several washes through the manifold with 10× SSC, blots were UV-cross-linked and probed with a radiolabeled ∼350-bp BglII–XhoI cDNA fragment of mo/huAPP695 cDNA.
T3754	8200-8205	IN	denotes	After
T3756	8206-8213	JJ	denotes	several
T3757	8214-8220	NNS	denotes	washes
T3758	8221-8228	IN	denotes	through
T3759	8229-8232	DT	denotes	the
T3760	8233-8241	NN	denotes	manifold
T3761	8242-8246	IN	denotes	with
T3762	8247-8249	CD	denotes	10
T3764	8249-8250	SYM	denotes	×
T3763	8251-8254	NN	denotes	SSC
T3765	8254-8256	,	denotes	,
T3766	8256-8261	NNS	denotes	blots
T3767	8262-8266	VBD	denotes	were
T3768	8267-8269	NN	denotes	UV
T3769	8269-8270	HYPH	denotes	-
T3755	8270-8282	VBN	denotes	cross-linked
T3770	8283-8286	CC	denotes	and
T3771	8287-8293	VBN	denotes	probed
T3772	8294-8298	IN	denotes	with
T3773	8299-8300	DT	denotes	a
T3775	8301-8313	VBN	denotes	radiolabeled
T3776	8314-8315	SYM	denotes	∼
T3777	8315-8318	CD	denotes	350
T3779	8318-8319	HYPH	denotes	-
T3778	8319-8321	NN	denotes	bp
T3780	8322-8327	NN	denotes	BglII
T3782	8327-8328	HYPH	denotes	–
T3781	8328-8332	NN	denotes	XhoI
T3783	8333-8337	NN	denotes	cDNA
T3774	8338-8346	NN	denotes	fragment
T3784	8347-8349	IN	denotes	of
T3785	8350-8352	NN	denotes	mo
T3787	8352-8353	HYPH	denotes	/
T3786	8353-8361	NN	denotes	huAPP695
T3788	8362-8366	NN	denotes	cDNA
T3789	8366-8367	.	denotes	.
T3790	8367-8699	sentence	denotes	After hybridizing overnight at 65 °C in 1% BSA/1 mM EDTA/0.5 M sodium phosphate buffer (pH 7.2)/7% SDS [20], the blots were washed twice at 65 °C for 30 min each in 0.1% BSA/1 mM EDTA/40 mM sodium phosphate buffer (pH 7.2)/5% SDS before two final 30-min washes at 65 °C with 1 mM EDTA/40 mM sodium phosphate buffer (pH 7.2)/1% SDS.
T3791	8368-8373	IN	denotes	After
T3793	8374-8385	VBG	denotes	hybridizing
T3794	8386-8395	RB	denotes	overnight
T3795	8396-8398	IN	denotes	at
T3796	8399-8401	CD	denotes	65
T3797	8402-8404	NN	denotes	°C
T3798	8405-8407	IN	denotes	in
T3799	8408-8409	CD	denotes	1
T3800	8409-8410	NN	denotes	%
T3801	8411-8414	NN	denotes	BSA
T3802	8414-8415	HYPH	denotes	/
T3803	8415-8416	CD	denotes	1
T3804	8417-8419	NN	denotes	mM
T3805	8420-8424	NN	denotes	EDTA
T3806	8424-8425	HYPH	denotes	/
T3807	8425-8428	CD	denotes	0.5
T3808	8429-8430	NN	denotes	M
T3810	8431-8437	NN	denotes	sodium
T3811	8438-8447	NN	denotes	phosphate
T3809	8448-8454	NN	denotes	buffer
T3812	8455-8456	-LRB-	denotes	(
T3813	8456-8458	NN	denotes	pH
T3814	8459-8462	CD	denotes	7.2
T3815	8462-8463	-RRB-	denotes	)
T3816	8463-8464	HYPH	denotes	/
T3817	8464-8465	CD	denotes	7
T3818	8465-8466	NN	denotes	%
T3819	8467-8470	NN	denotes	SDS
T3820	8471-8472	-LRB-	denotes	[
T3821	8472-8474	CD	denotes	20
T3822	8474-8475	-RRB-	denotes	]
T3823	8475-8477	,	denotes	,
T3824	8477-8480	DT	denotes	the
T3825	8481-8486	NNS	denotes	blots
T3826	8487-8491	VBD	denotes	were
T3792	8492-8498	VBN	denotes	washed
T3827	8499-8504	RB	denotes	twice
T3828	8505-8507	IN	denotes	at
T3829	8508-8510	CD	denotes	65
T3830	8511-8513	NN	denotes	°C
T3831	8514-8517	IN	denotes	for
T3832	8518-8520	CD	denotes	30
T3833	8521-8524	NN	denotes	min
T3834	8525-8529	DT	denotes	each
T3835	8530-8532	IN	denotes	in
T3836	8533-8536	CD	denotes	0.1
T3837	8536-8537	NN	denotes	%
T3838	8538-8541	NN	denotes	BSA
T3839	8541-8542	HYPH	denotes	/
T3840	8542-8543	CD	denotes	1
T3841	8544-8546	NN	denotes	mM
T3842	8547-8551	NN	denotes	EDTA
T3843	8551-8552	HYPH	denotes	/
T3844	8552-8554	CD	denotes	40
T3845	8555-8557	NN	denotes	mM
T3847	8558-8564	NN	denotes	sodium
T3848	8565-8574	NN	denotes	phosphate
T3846	8575-8581	NN	denotes	buffer
T3849	8582-8583	-LRB-	denotes	(
T3850	8583-8585	NN	denotes	pH
T3851	8586-8589	CD	denotes	7.2
T3852	8589-8590	-RRB-	denotes	)
T3853	8590-8591	HYPH	denotes	/
T3854	8591-8592	CD	denotes	5
T3855	8592-8593	NN	denotes	%
T3856	8594-8597	NN	denotes	SDS
T3857	8598-8604	IN	denotes	before
T3858	8605-8608	CD	denotes	two
T3860	8609-8614	JJ	denotes	final
T3861	8615-8617	CD	denotes	30
T3863	8617-8618	HYPH	denotes	-
T3862	8618-8621	NN	denotes	min
T3859	8622-8628	NNS	denotes	washes
T3864	8629-8631	IN	denotes	at
T3865	8632-8634	CD	denotes	65
T3866	8635-8637	NN	denotes	°C
T3867	8638-8642	IN	denotes	with
T3868	8643-8644	CD	denotes	1
T3869	8645-8647	NN	denotes	mM
T3870	8648-8652	NN	denotes	EDTA
T3871	8652-8653	HYPH	denotes	/
T3872	8653-8655	CD	denotes	40
T3873	8656-8658	NN	denotes	mM
T3875	8659-8665	NN	denotes	sodium
T3876	8666-8675	NN	denotes	phosphate
T3874	8676-8682	NN	denotes	buffer
T3877	8683-8684	-LRB-	denotes	(
T3878	8684-8686	NN	denotes	pH
T3879	8687-8690	CD	denotes	7.2
T3880	8690-8691	-RRB-	denotes	)
T3881	8691-8692	HYPH	denotes	/
T3882	8692-8693	CD	denotes	1
T3883	8693-8694	NN	denotes	%
T3884	8695-8698	NN	denotes	SDS
T3885	8698-8699	.	denotes	.
T3886	8699-8784	sentence	denotes	Blots were wrapped wet and exposed to phosphorscreens overnight at room temperature.
T3887	8700-8705	NNS	denotes	Blots
T3889	8706-8710	VBD	denotes	were
T3888	8711-8718	VBN	denotes	wrapped
T3890	8719-8722	JJ	denotes	wet
T3891	8723-8726	CC	denotes	and
T3892	8727-8734	VBN	denotes	exposed
T3893	8735-8737	IN	denotes	to
T3894	8738-8753	NNS	denotes	phosphorscreens
T3895	8754-8763	RB	denotes	overnight
T3896	8764-8766	IN	denotes	at
T3897	8767-8771	NN	denotes	room
T3898	8772-8783	NN	denotes	temperature
T3899	8783-8784	.	denotes	.
T4137	8786-8793	NN	denotes	Amyloid
T4138	8794-8803	NN	denotes	Histology
T4139	8803-8917	sentence	denotes	Mice were euthanized by ether inhalation and brains removed for immersion fixation in 4% paraformaldehyde/1× PBS.
T4140	8804-8808	NNS	denotes	Mice
T4142	8809-8813	VBD	denotes	were
T4141	8814-8824	VBN	denotes	euthanized
T4143	8825-8827	IN	denotes	by
T4144	8828-8833	NN	denotes	ether
T4145	8834-8844	NN	denotes	inhalation
T4146	8845-8848	CC	denotes	and
T4147	8849-8855	NNS	denotes	brains
T4148	8856-8863	VBN	denotes	removed
T4149	8864-8867	IN	denotes	for
T4150	8868-8877	NN	denotes	immersion
T4151	8878-8886	NN	denotes	fixation
T4152	8887-8889	IN	denotes	in
T4153	8890-8891	CD	denotes	4
T4154	8891-8892	NN	denotes	%
T4155	8893-8909	NN	denotes	paraformaldehyde
T4156	8909-8910	HYPH	denotes	/
T4157	8910-8911	CD	denotes	1
T4159	8911-8912	SYM	denotes	×
T4158	8913-8916	NN	denotes	PBS
T4160	8916-8917	.	denotes	.
T4161	8917-9095	sentence	denotes	After 48 h in fixative at 4 °C, brains were transferred to PBS, dehydrated in alcohols, treated with cedarwood oil and methylsalicylate, and embedded in paraffin for sectioning.
T4162	8918-8923	IN	denotes	After
T4164	8924-8926	CD	denotes	48
T4165	8927-8928	NN	denotes	h
T4166	8929-8931	IN	denotes	in
T4167	8932-8940	JJ	denotes	fixative
T4168	8941-8943	IN	denotes	at
T4169	8944-8945	CD	denotes	4
T4170	8946-8948	NN	denotes	°C
T4171	8948-8950	,	denotes	,
T4172	8950-8956	NNS	denotes	brains
T4173	8957-8961	VBD	denotes	were
T4163	8962-8973	VBN	denotes	transferred
T4174	8974-8976	IN	denotes	to
T4175	8977-8980	NN	denotes	PBS
T4176	8980-8982	,	denotes	,
T4177	8982-8992	VBN	denotes	dehydrated
T4178	8993-8995	IN	denotes	in
T4179	8996-9004	NNS	denotes	alcohols
T4180	9004-9006	,	denotes	,
T4181	9006-9013	VBN	denotes	treated
T4182	9014-9018	IN	denotes	with
T4183	9019-9028	NN	denotes	cedarwood
T4184	9029-9032	NN	denotes	oil
T4185	9033-9036	CC	denotes	and
T4186	9037-9053	NN	denotes	methylsalicylate
T4187	9053-9055	,	denotes	,
T4188	9055-9058	CC	denotes	and
T4189	9059-9067	VBN	denotes	embedded
T4190	9068-9070	IN	denotes	in
T4191	9071-9079	NN	denotes	paraffin
T4192	9080-9083	IN	denotes	for
T4193	9084-9094	VBG	denotes	sectioning
T4194	9094-9095	.	denotes	.
T4195	9095-9116	sentence	denotes	Hirano silver stain
T4196	9097-9103	NN	denotes	Hirano
T4198	9104-9110	NN	denotes	silver
T4197	9111-9116	NN	denotes	stain
T4199	9116-9254	sentence	denotes	Silver impregnation histology was performed on 10-μm paraffin-embedded sections by Hirano's modification of the Bielschowsky method [21].
T4200	9117-9123	NN	denotes	Silver
T4202	9124-9136	NN	denotes	impregnation
T4201	9137-9146	NN	denotes	histology
T4204	9147-9150	VBD	denotes	was
T4203	9151-9160	VBN	denotes	performed
T4205	9161-9163	IN	denotes	on
T4206	9164-9166	CD	denotes	10
T4208	9166-9167	HYPH	denotes	-
T4207	9167-9169	NN	denotes	μm
T4210	9170-9178	NN	denotes	paraffin
T4212	9178-9179	HYPH	denotes	-
T4211	9179-9187	VBN	denotes	embedded
T4209	9188-9196	NNS	denotes	sections
T4213	9197-9199	IN	denotes	by
T4214	9200-9206	NNP	denotes	Hirano
T4216	9206-9208	POS	denotes	's
T4215	9209-9221	NN	denotes	modification
T4217	9222-9224	IN	denotes	of
T4218	9225-9228	DT	denotes	the
T4220	9229-9241	NNP	denotes	Bielschowsky
T4219	9242-9248	NN	denotes	method
T4221	9249-9250	-LRB-	denotes	[
T4222	9250-9252	CD	denotes	21
T4223	9252-9253	-RRB-	denotes	]
T4224	9253-9254	.	denotes	.
T4225	9254-9406	sentence	denotes	Briefly, sections were deparaffinized through xylene and alcohols into tap water before being placed into fresh 20% silver nitrate solution for 20 min.
T4226	9255-9262	RB	denotes	Briefly
T4228	9262-9264	,	denotes	,
T4229	9264-9272	NNS	denotes	sections
T4230	9273-9277	VBD	denotes	were
T4227	9278-9292	VBN	denotes	deparaffinized
T4231	9293-9300	IN	denotes	through
T4232	9301-9307	NN	denotes	xylene
T4233	9308-9311	CC	denotes	and
T4234	9312-9320	NNS	denotes	alcohols
T4235	9321-9325	IN	denotes	into
T4236	9326-9329	NN	denotes	tap
T4237	9330-9335	NN	denotes	water
T4238	9336-9342	IN	denotes	before
T4239	9343-9348	VBG	denotes	being
T4240	9349-9355	VBN	denotes	placed
T4241	9356-9360	IN	denotes	into
T4242	9361-9366	JJ	denotes	fresh
T4244	9367-9369	CD	denotes	20
T4245	9369-9370	NN	denotes	%
T4246	9371-9377	NN	denotes	silver
T4247	9378-9385	NN	denotes	nitrate
T4243	9386-9394	NN	denotes	solution
T4248	9395-9398	IN	denotes	for
T4249	9399-9401	CD	denotes	20
T4250	9402-9405	NN	denotes	min
T4251	9405-9406	.	denotes	.
T4252	9406-9550	sentence	denotes	After being washed thoroughly with distilled water, slides were immersed in 20% silver nitrate solution titrated with fresh ammonium hydroxide.
T4253	9407-9412	IN	denotes	After
T4255	9413-9418	VBG	denotes	being
T4256	9419-9425	VBN	denotes	washed
T4257	9426-9436	RB	denotes	thoroughly
T4258	9437-9441	IN	denotes	with
T4259	9442-9451	JJ	denotes	distilled
T4260	9452-9457	NN	denotes	water
T4261	9457-9459	,	denotes	,
T4262	9459-9465	NNS	denotes	slides
T4263	9466-9470	VBD	denotes	were
T4254	9471-9479	VBN	denotes	immersed
T4264	9480-9482	IN	denotes	in
T4265	9483-9485	CD	denotes	20
T4266	9485-9486	NN	denotes	%
T4268	9487-9493	NN	denotes	silver
T4269	9494-9501	NN	denotes	nitrate
T4267	9502-9510	NN	denotes	solution
T4270	9511-9519	VBN	denotes	titrated
T4271	9520-9524	IN	denotes	with
T4272	9525-9530	JJ	denotes	fresh
T4274	9531-9539	NN	denotes	ammonium
T4273	9540-9549	NN	denotes	hydroxide
T4275	9549-9550	.	denotes	.
T4276	9550-9831	sentence	denotes	After 20 min, slides were washed with ammonia water before being individually developed with 100 μl of developer (20 ml of 37% formaldehyde, 100 ml of distilled water, 50 μl of concentrated nitric acid, and 0.5 g of citric acid) added to 50 ml of titrated silver nitrate solution.
T4277	9551-9556	IN	denotes	After
T4279	9557-9559	CD	denotes	20
T4280	9560-9563	NN	denotes	min
T4281	9563-9565	,	denotes	,
T4282	9565-9571	NNS	denotes	slides
T4283	9572-9576	VBD	denotes	were
T4278	9577-9583	VBN	denotes	washed
T4284	9584-9588	IN	denotes	with
T4285	9589-9596	NN	denotes	ammonia
T4286	9597-9602	NN	denotes	water
T4287	9603-9609	IN	denotes	before
T4288	9610-9615	VBG	denotes	being
T4290	9616-9628	RB	denotes	individually
T4289	9629-9638	VBN	denotes	developed
T4291	9639-9643	IN	denotes	with
T4292	9644-9647	CD	denotes	100
T4293	9648-9650	NN	denotes	μl
T4294	9651-9653	IN	denotes	of
T4295	9654-9663	NN	denotes	developer
T4296	9664-9665	-LRB-	denotes	(
T4298	9665-9667	CD	denotes	20
T4297	9668-9670	NN	denotes	ml
T4299	9671-9673	IN	denotes	of
T4300	9674-9676	CD	denotes	37
T4301	9676-9677	NN	denotes	%
T4302	9678-9690	NN	denotes	formaldehyde
T4303	9690-9692	,	denotes	,
T4304	9692-9695	CD	denotes	100
T4305	9696-9698	NN	denotes	ml
T4306	9699-9701	IN	denotes	of
T4307	9702-9711	JJ	denotes	distilled
T4308	9712-9717	NN	denotes	water
T4309	9717-9719	,	denotes	,
T4310	9719-9721	CD	denotes	50
T4311	9722-9724	NN	denotes	μl
T4312	9725-9727	IN	denotes	of
T4313	9728-9740	VBN	denotes	concentrated
T4315	9741-9747	JJ	denotes	nitric
T4314	9748-9752	NN	denotes	acid
T4316	9752-9754	,	denotes	,
T4317	9754-9757	CC	denotes	and
T4318	9758-9761	CD	denotes	0.5
T4319	9762-9763	NN	denotes	g
T4320	9764-9766	IN	denotes	of
T4321	9767-9773	JJ	denotes	citric
T4322	9774-9778	NN	denotes	acid
T4323	9778-9779	-RRB-	denotes	)
T4324	9780-9785	VBN	denotes	added
T4325	9786-9788	IN	denotes	to
T4326	9789-9791	CD	denotes	50
T4327	9792-9794	NN	denotes	ml
T4328	9795-9797	IN	denotes	of
T4329	9798-9806	VBN	denotes	titrated
T4331	9807-9813	NN	denotes	silver
T4332	9814-9821	NN	denotes	nitrate
T4330	9822-9830	NN	denotes	solution
T4333	9830-9831	.	denotes	.
T4334	9831-9945	sentence	denotes	Slides were then rinsed in tap water, fixed in 5% sodium thiosulfate, and dehydrated through alcohols and xylene.
T4335	9832-9838	NNS	denotes	Slides
T4337	9839-9843	VBD	denotes	were
T4338	9844-9848	RB	denotes	then
T4336	9849-9855	VBN	denotes	rinsed
T4339	9856-9858	IN	denotes	in
T4340	9859-9862	NN	denotes	tap
T4341	9863-9868	NN	denotes	water
T4342	9868-9870	,	denotes	,
T4343	9870-9875	VBN	denotes	fixed
T4344	9876-9878	IN	denotes	in
T4345	9879-9880	CD	denotes	5
T4346	9880-9881	NN	denotes	%
T4348	9882-9888	NN	denotes	sodium
T4347	9889-9900	NN	denotes	thiosulfate
T4349	9900-9902	,	denotes	,
T4350	9902-9905	CC	denotes	and
T4351	9906-9916	VBN	denotes	dehydrated
T4352	9917-9924	IN	denotes	through
T4353	9925-9933	NNS	denotes	alcohols
T4354	9934-9937	CC	denotes	and
T4355	9938-9944	NN	denotes	xylene
T4356	9944-9945	.	denotes	.
T4357	9945-9968	sentence	denotes	Thioflavin-S staining
T4358	9947-9957	NN	denotes	Thioflavin
T4360	9957-9958	HYPH	denotes	-
T4359	9958-9959	NN	denotes	S
T4361	9960-9968	NN	denotes	staining
T4362	9968-10154	sentence	denotes	Following deparaffinization of sections through xylene and alcohols, amyloid impregnation with thioflavin-S was performed according to the Guntern modification of the standard protocol.
T4363	9969-9978	VBG	denotes	Following
T4365	9979-9996	NN	denotes	deparaffinization
T4366	9997-9999	IN	denotes	of
T4367	10000-10008	NNS	denotes	sections
T4368	10009-10016	IN	denotes	through
T4369	10017-10023	NN	denotes	xylene
T4370	10024-10027	CC	denotes	and
T4371	10028-10036	NNS	denotes	alcohols
T4372	10036-10038	,	denotes	,
T4373	10038-10045	NN	denotes	amyloid
T4374	10046-10058	NN	denotes	impregnation
T4375	10059-10063	IN	denotes	with
T4376	10064-10074	NN	denotes	thioflavin
T4378	10074-10075	HYPH	denotes	-
T4377	10075-10076	NN	denotes	S
T4379	10077-10080	VBD	denotes	was
T4364	10081-10090	VBN	denotes	performed
T4380	10091-10100	VBG	denotes	according
T4381	10101-10103	IN	denotes	to
T4382	10104-10107	DT	denotes	the
T4384	10108-10115	NN	denotes	Guntern
T4383	10116-10128	NN	denotes	modification
T4385	10129-10131	IN	denotes	of
T4386	10132-10135	DT	denotes	the
T4388	10136-10144	JJ	denotes	standard
T4387	10145-10153	NN	denotes	protocol
T4389	10153-10154	.	denotes	.
T4390	10154-10374	sentence	denotes	Slides holding 10-μm paraffin sections were washed twice in distilled water, then immersed for 5 min in a 0.25% potassium permanganate solution, followed by 5 min in a 1% potassium metabisulfate/1% oxalic acid solution.
T4391	10155-10161	NNS	denotes	Slides
T4393	10162-10169	VBG	denotes	holding
T4394	10170-10172	CD	denotes	10
T4396	10172-10173	HYPH	denotes	-
T4395	10173-10175	NN	denotes	μm
T4398	10176-10184	NN	denotes	paraffin
T4397	10185-10193	NNS	denotes	sections
T4399	10194-10198	VBD	denotes	were
T4392	10199-10205	VBN	denotes	washed
T4400	10206-10211	RB	denotes	twice
T4401	10212-10214	IN	denotes	in
T4402	10215-10224	JJ	denotes	distilled
T4403	10225-10230	NN	denotes	water
T4404	10230-10232	,	denotes	,
T4405	10232-10236	RB	denotes	then
T4406	10237-10245	VBN	denotes	immersed
T4407	10246-10249	IN	denotes	for
T4408	10250-10251	CD	denotes	5
T4409	10252-10255	NN	denotes	min
T4410	10256-10258	IN	denotes	in
T4411	10259-10260	DT	denotes	a
T4413	10261-10265	CD	denotes	0.25
T4414	10265-10266	NN	denotes	%
T4415	10267-10276	NN	denotes	potassium
T4416	10277-10289	NN	denotes	permanganate
T4412	10290-10298	NN	denotes	solution
T4417	10298-10300	,	denotes	,
T4418	10300-10308	VBN	denotes	followed
T4419	10309-10311	IN	denotes	by
T4420	10312-10313	CD	denotes	5
T4421	10314-10317	NN	denotes	min
T4422	10318-10320	IN	denotes	in
T4423	10321-10322	DT	denotes	a
T4425	10323-10324	CD	denotes	1
T4426	10324-10325	NN	denotes	%
T4428	10326-10335	NN	denotes	potassium
T4427	10336-10349	NN	denotes	metabisulfate
T4429	10349-10350	HYPH	denotes	/
T4430	10350-10351	CD	denotes	1
T4431	10351-10352	NN	denotes	%
T4433	10353-10359	JJ	denotes	oxalic
T4432	10360-10364	NN	denotes	acid
T4424	10365-10373	NN	denotes	solution
T4434	10373-10374	.	denotes	.
T4435	10374-10526	sentence	denotes	After this preparation, slides were placed into a filtered aqueous 0.02% thioflavin-S solution (Chroma-Gesellschaft Schmid, Kongen, Germany) for 8 min.
T4436	10375-10380	IN	denotes	After
T4438	10381-10385	DT	denotes	this
T4439	10386-10397	NN	denotes	preparation
T4440	10397-10399	,	denotes	,
T4441	10399-10405	NNS	denotes	slides
T4442	10406-10410	VBD	denotes	were
T4437	10411-10417	VBN	denotes	placed
T4443	10418-10422	IN	denotes	into
T4444	10423-10424	DT	denotes	a
T4446	10425-10433	VBN	denotes	filtered
T4447	10434-10441	JJ	denotes	aqueous
T4448	10442-10446	CD	denotes	0.02
T4449	10446-10447	NN	denotes	%
T4450	10448-10458	NN	denotes	thioflavin
T4452	10458-10459	HYPH	denotes	-
T4451	10459-10460	NN	denotes	S
T4445	10461-10469	NN	denotes	solution
T4453	10470-10471	-LRB-	denotes	(
T4455	10471-10477	NNP	denotes	Chroma
T4457	10477-10478	HYPH	denotes	-
T4456	10478-10490	NNP	denotes	Gesellschaft
T4454	10491-10497	NNP	denotes	Schmid
T4458	10497-10499	,	denotes	,
T4459	10499-10505	NNP	denotes	Kongen
T4460	10505-10507	,	denotes	,
T4461	10507-10514	NNP	denotes	Germany
T4462	10514-10515	-RRB-	denotes	)
T4463	10516-10519	IN	denotes	for
T4464	10520-10521	CD	denotes	8
T4465	10522-10525	NN	denotes	min
T4466	10525-10526	.	denotes	.
T4467	10526-10710	sentence	denotes	Excess stain was removed by two brief rinses in 80% ethanol, then two in distilled water, after which slides were finished in aqueous mounting medium for florescence photomicrography.
T4468	10527-10533	JJ	denotes	Excess
T4469	10534-10539	NN	denotes	stain
T4471	10540-10543	VBD	denotes	was
T4470	10544-10551	VBN	denotes	removed
T4472	10552-10554	IN	denotes	by
T4473	10555-10558	CD	denotes	two
T4475	10559-10564	JJ	denotes	brief
T4474	10565-10571	NNS	denotes	rinses
T4476	10572-10574	IN	denotes	in
T4477	10575-10577	CD	denotes	80
T4478	10577-10578	NN	denotes	%
T4479	10579-10586	NN	denotes	ethanol
T4480	10586-10588	,	denotes	,
T4481	10588-10592	RB	denotes	then
T4482	10593-10596	CD	denotes	two
T4483	10597-10599	IN	denotes	in
T4484	10600-10609	JJ	denotes	distilled
T4485	10610-10615	NN	denotes	water
T4486	10615-10617	,	denotes	,
T4487	10617-10622	IN	denotes	after
T4489	10623-10628	WDT	denotes	which
T4490	10629-10635	NNS	denotes	slides
T4491	10636-10640	VBD	denotes	were
T4488	10641-10649	VBN	denotes	finished
T4492	10650-10652	IN	denotes	in
T4493	10653-10660	JJ	denotes	aqueous
T4495	10661-10669	NN	denotes	mounting
T4494	10670-10676	NN	denotes	medium
T4496	10677-10680	IN	denotes	for
T4497	10681-10692	NN	denotes	florescence
T4498	10693-10709	NN	denotes	photomicrography
T4499	10709-10710	.	denotes	.
T4500	10710-10783	sentence	denotes	Ubiquitin, glial fibrillary acidic protein, and Aβ immunohistochemistry
T4501	10712-10721	NN	denotes	Ubiquitin
T4502	10721-10723	,	denotes	,
T4503	10723-10728	JJ	denotes	glial
T4505	10729-10739	JJ	denotes	fibrillary
T4506	10740-10746	JJ	denotes	acidic
T4504	10747-10754	NN	denotes	protein
T4507	10754-10756	,	denotes	,
T4508	10756-10759	CC	denotes	and
T4509	10760-10762	NN	denotes	Aβ
T4510	10763-10783	NN	denotes	immunohistochemistry
T4511	10783-10884	sentence	denotes	Prior to immunostaining, slides were deparaffinized by oven heating followed by immersion in xylene.
T4512	10784-10789	JJ	denotes	Prior
T4513	10790-10792	IN	denotes	to
T4515	10793-10807	NN	denotes	immunostaining
T4516	10807-10809	,	denotes	,
T4517	10809-10815	NNS	denotes	slides
T4518	10816-10820	VBD	denotes	were
T4514	10821-10835	VBN	denotes	deparaffinized
T4519	10836-10838	IN	denotes	by
T4520	10839-10843	NN	denotes	oven
T4521	10844-10851	NN	denotes	heating
T4522	10852-10860	VBN	denotes	followed
T4523	10861-10863	IN	denotes	by
T4524	10864-10873	NN	denotes	immersion
T4525	10874-10876	IN	denotes	in
T4526	10877-10883	NN	denotes	xylene
T4527	10883-10884	.	denotes	.
T4528	10884-11039	sentence	denotes	After rehydration through graded alcohols into tap water, endogenous peroxidase activity was quenched by incubation with 3% hydrogen peroxide in methanol.
T4529	10885-10890	IN	denotes	After
T4531	10891-10902	NN	denotes	rehydration
T4532	10903-10910	IN	denotes	through
T4533	10911-10917	VBN	denotes	graded
T4534	10918-10926	NNS	denotes	alcohols
T4535	10927-10931	IN	denotes	into
T4536	10932-10935	NN	denotes	tap
T4537	10936-10941	NN	denotes	water
T4538	10941-10943	,	denotes	,
T4539	10943-10953	JJ	denotes	endogenous
T4541	10954-10964	NN	denotes	peroxidase
T4540	10965-10973	NN	denotes	activity
T4542	10974-10977	VBD	denotes	was
T4530	10978-10986	VBN	denotes	quenched
T4543	10987-10989	IN	denotes	by
T4544	10990-11000	NN	denotes	incubation
T4545	11001-11005	IN	denotes	with
T4546	11006-11007	CD	denotes	3
T4547	11007-11008	NN	denotes	%
T4549	11009-11017	NN	denotes	hydrogen
T4548	11018-11026	NN	denotes	peroxide
T4550	11027-11029	IN	denotes	in
T4551	11030-11038	NN	denotes	methanol
T4552	11038-11039	.	denotes	.
T4553	11039-11122	sentence	denotes	Slides were microwaved for 5–7 min in water, cooled for 5 min, then washed in TBS.
T4554	11040-11046	NNS	denotes	Slides
T4556	11047-11051	VBD	denotes	were
T4555	11052-11062	VBN	denotes	microwaved
T4557	11063-11066	IN	denotes	for
T4558	11067-11068	CD	denotes	5
T4560	11068-11069	SYM	denotes	–
T4559	11069-11070	CD	denotes	7
T4561	11071-11074	NN	denotes	min
T4562	11075-11077	IN	denotes	in
T4563	11078-11083	NN	denotes	water
T4564	11083-11085	,	denotes	,
T4565	11085-11091	VBN	denotes	cooled
T4566	11092-11095	IN	denotes	for
T4567	11096-11097	CD	denotes	5
T4568	11098-11101	NN	denotes	min
T4569	11101-11103	,	denotes	,
T4570	11103-11107	RB	denotes	then
T4571	11108-11114	VBN	denotes	washed
T4572	11115-11117	IN	denotes	in
T4573	11118-11121	NN	denotes	TBS
T4574	11121-11122	.	denotes	.
T4575	11122-11219	sentence	denotes	Nonspecific staining was blocked for 1 h with 3% normal goat serum and 0.1% Triton-X 100 in TBS.
T4576	11123-11134	JJ	denotes	Nonspecific
T4577	11135-11143	NN	denotes	staining
T4579	11144-11147	VBD	denotes	was
T4578	11148-11155	VBN	denotes	blocked
T4580	11156-11159	IN	denotes	for
T4581	11160-11161	CD	denotes	1
T4582	11162-11163	NN	denotes	h
T4583	11164-11168	IN	denotes	with
T4584	11169-11170	CD	denotes	3
T4585	11170-11171	NN	denotes	%
T4587	11172-11178	JJ	denotes	normal
T4588	11179-11183	NN	denotes	goat
T4586	11184-11189	NN	denotes	serum
T4589	11190-11193	CC	denotes	and
T4590	11194-11197	CD	denotes	0.1
T4591	11197-11198	NN	denotes	%
T4593	11199-11205	NN	denotes	Triton
T4594	11205-11206	HYPH	denotes	-
T4592	11206-11207	NN	denotes	X
T4595	11208-11211	CD	denotes	100
T4596	11212-11214	IN	denotes	in
T4597	11215-11218	NN	denotes	TBS
T4598	11218-11219	.	denotes	.
T4599	11219-11616	sentence	denotes	Slides were then placed into primary antibody (rabbit anti-Aβ peptide polyclonal antibody, Zymed Laboratories, South San Francisco, California, United States; rabbit anti-ubiquitin and rabbit anti–glial fibrillary acidic protein (GFAP) polyclonal antibodies, Dako, Carpinteria, California, United States) diluted 1:500 in TBS with 2% normal goat serum and incubated overnight at room temperature.
T4600	11220-11226	NNS	denotes	Slides
T4602	11227-11231	VBD	denotes	were
T4603	11232-11236	RB	denotes	then
T4601	11237-11243	VBN	denotes	placed
T4604	11244-11248	IN	denotes	into
T4605	11249-11256	JJ	denotes	primary
T4606	11257-11265	NN	denotes	antibody
T4607	11266-11267	-LRB-	denotes	(
T4609	11267-11273	NN	denotes	rabbit
T4611	11274-11281	JJ	denotes	anti-Aβ
T4612	11282-11289	NN	denotes	peptide
T4613	11290-11300	JJ	denotes	polyclonal
T4610	11301-11309	NN	denotes	antibody
T4614	11309-11311	,	denotes	,
T4615	11311-11316	NNP	denotes	Zymed
T4616	11317-11329	NNP	denotes	Laboratories
T4617	11329-11331	,	denotes	,
T4618	11331-11336	NNP	denotes	South
T4620	11337-11340	NNP	denotes	San
T4619	11341-11350	NNP	denotes	Francisco
T4621	11350-11352	,	denotes	,
T4622	11352-11362	NNP	denotes	California
T4623	11362-11364	,	denotes	,
T4624	11364-11370	NNP	denotes	United
T4625	11371-11377	NNP	denotes	States
T4626	11377-11378	:	denotes	;
T4627	11379-11385	NN	denotes	rabbit
T4628	11386-11400	JJ	denotes	anti-ubiquitin
T4629	11401-11404	CC	denotes	and
T4630	11405-11411	NN	denotes	rabbit
T4631	11412-11422	JJ	denotes	anti–glial
T4632	11423-11433	JJ	denotes	fibrillary
T4634	11434-11440	JJ	denotes	acidic
T4633	11441-11448	NN	denotes	protein
T4635	11449-11450	-LRB-	denotes	(
T4636	11450-11454	NN	denotes	GFAP
T4637	11454-11455	-RRB-	denotes	)
T4638	11456-11466	JJ	denotes	polyclonal
T4608	11467-11477	NNS	denotes	antibodies
T4639	11477-11479	,	denotes	,
T4640	11479-11483	NNP	denotes	Dako
T4641	11483-11485	,	denotes	,
T4642	11485-11496	NNP	denotes	Carpinteria
T4643	11496-11498	,	denotes	,
T4644	11498-11508	NNP	denotes	California
T4645	11508-11510	,	denotes	,
T4646	11510-11516	NNP	denotes	United
T4647	11517-11523	NNP	denotes	States
T4648	11523-11524	-RRB-	denotes	)
T4649	11525-11532	VBN	denotes	diluted
T4650	11533-11534	CD	denotes	1
T4651	11534-11535	SYM	denotes	:
T4652	11535-11538	CD	denotes	500
T4653	11539-11541	IN	denotes	in
T4654	11542-11545	NN	denotes	TBS
T4655	11546-11550	IN	denotes	with
T4656	11551-11552	CD	denotes	2
T4657	11552-11553	NN	denotes	%
T4659	11554-11560	JJ	denotes	normal
T4660	11561-11565	NN	denotes	goat
T4658	11566-11571	NN	denotes	serum
T4661	11572-11575	CC	denotes	and
T4662	11576-11585	VBN	denotes	incubated
T4663	11586-11595	RB	denotes	overnight
T4664	11596-11598	IN	denotes	at
T4665	11599-11603	NN	denotes	room
T4666	11604-11615	NN	denotes	temperature
T4667	11615-11616	.	denotes	.
T4668	11616-12023	sentence	denotes	After being washed of excess primary antibody with several changes of TBS, slides were incubated with either the Vectastain Elite anti-rabbit secondary system (anti-Aβ; Vector Laboratories, Burlingame, California, United States) or peroxidase/anti-peroxidase reagents (anti-ubitquitin and anti-GFAP; Sternberger Monoclonals, Lutherville, Maryland, United States) according to the manufacturers' directions.
T4669	11617-11622	IN	denotes	After
T4671	11623-11628	VBG	denotes	being
T4672	11629-11635	VBN	denotes	washed
T4673	11636-11638	IN	denotes	of
T4674	11639-11645	JJ	denotes	excess
T4676	11646-11653	JJ	denotes	primary
T4675	11654-11662	NN	denotes	antibody
T4677	11663-11667	IN	denotes	with
T4678	11668-11675	JJ	denotes	several
T4679	11676-11683	NNS	denotes	changes
T4680	11684-11686	IN	denotes	of
T4681	11687-11690	NN	denotes	TBS
T4682	11690-11692	,	denotes	,
T4683	11692-11698	NNS	denotes	slides
T4684	11699-11703	VBD	denotes	were
T4670	11704-11713	VBN	denotes	incubated
T4685	11714-11718	IN	denotes	with
T4686	11719-11725	CC	denotes	either
T4688	11726-11729	DT	denotes	the
T4689	11730-11740	NNP	denotes	Vectastain
T4690	11741-11746	NNP	denotes	Elite
T4691	11747-11758	JJ	denotes	anti-rabbit
T4692	11759-11768	JJ	denotes	secondary
T4687	11769-11775	NN	denotes	system
T4693	11776-11777	-LRB-	denotes	(
T4695	11777-11784	JJ	denotes	anti-Aβ
T4696	11784-11785	:	denotes	;
T4697	11786-11792	NNP	denotes	Vector
T4694	11793-11805	NNP	denotes	Laboratories
T4698	11805-11807	,	denotes	,
T4699	11807-11817	NNP	denotes	Burlingame
T4700	11817-11819	,	denotes	,
T4701	11819-11829	NNP	denotes	California
T4702	11829-11831	,	denotes	,
T4703	11831-11837	NNP	denotes	United
T4704	11838-11844	NNP	denotes	States
T4705	11844-11845	-RRB-	denotes	)
T4706	11846-11848	CC	denotes	or
T4707	11849-11859	NN	denotes	peroxidase
T4709	11859-11860	HYPH	denotes	/
T4710	11860-11875	JJ	denotes	anti-peroxidase
T4708	11876-11884	NNS	denotes	reagents
T4711	11885-11886	-LRB-	denotes	(
T4713	11886-11901	JJ	denotes	anti-ubitquitin
T4714	11902-11905	CC	denotes	and
T4715	11906-11915	JJ	denotes	anti-GFAP
T4716	11915-11916	:	denotes	;
T4717	11917-11928	NNP	denotes	Sternberger
T4712	11929-11940	NNPS	denotes	Monoclonals
T4718	11940-11942	,	denotes	,
T4719	11942-11953	NNP	denotes	Lutherville
T4720	11953-11955	,	denotes	,
T4721	11955-11963	NNP	denotes	Maryland
T4722	11963-11965	,	denotes	,
T4723	11965-11971	NNP	denotes	United
T4724	11972-11978	NNP	denotes	States
T4725	11978-11979	-RRB-	denotes	)
T4726	11980-11989	VBG	denotes	according
T4727	11990-11992	IN	denotes	to
T4728	11993-11996	DT	denotes	the
T4729	11997-12010	NNS	denotes	manufacturers
T4731	12010-12011	POS	denotes	'
T4730	12012-12022	NNS	denotes	directions
T4732	12022-12023	.	denotes	.
T4733	12023-12129	sentence	denotes	Antibody binding was visualized with diaminobenzidene, and sections were counterstained with hematoxylin.
T4734	12024-12032	NN	denotes	Antibody
T4735	12033-12040	NN	denotes	binding
T4737	12041-12044	VBD	denotes	was
T4736	12045-12055	VBN	denotes	visualized
T4738	12056-12060	IN	denotes	with
T4739	12061-12077	NN	denotes	diaminobenzidene
T4740	12077-12079	,	denotes	,
T4741	12079-12082	CC	denotes	and
T4742	12083-12091	NNS	denotes	sections
T4744	12092-12096	VBD	denotes	were
T4743	12097-12111	VBN	denotes	counterstained
T4745	12112-12116	IN	denotes	with
T4746	12117-12128	NN	denotes	hematoxylin
T4747	12128-12129	.	denotes	.
T4787	12131-12137	NN	denotes	Filter
T4789	12138-12142	NN	denotes	Trap
T4788	12143-12148	NN	denotes	Assay
T4790	12148-12300	sentence	denotes	An aliquot of each cortical homogenate used for Western blotting above was partially solubilized by the addition of SDS to a final concentration of 1%.
T4791	12149-12151	DT	denotes	An
T4792	12152-12159	NN	denotes	aliquot
T4794	12160-12162	IN	denotes	of
T4795	12163-12167	DT	denotes	each
T4797	12168-12176	JJ	denotes	cortical
T4796	12177-12187	NN	denotes	homogenate
T4798	12188-12192	VBN	denotes	used
T4799	12193-12196	IN	denotes	for
T4800	12197-12204	NNP	denotes	Western
T4801	12205-12213	NN	denotes	blotting
T4802	12214-12219	RB	denotes	above
T4803	12220-12223	VBD	denotes	was
T4804	12224-12233	RB	denotes	partially
T4793	12234-12245	VBN	denotes	solubilized
T4805	12246-12248	IN	denotes	by
T4806	12249-12252	DT	denotes	the
T4807	12253-12261	NN	denotes	addition
T4808	12262-12264	IN	denotes	of
T4809	12265-12268	NN	denotes	SDS
T4810	12269-12271	IN	denotes	to
T4811	12272-12273	DT	denotes	a
T4813	12274-12279	JJ	denotes	final
T4812	12280-12293	NN	denotes	concentration
T4814	12294-12296	IN	denotes	of
T4815	12297-12298	CD	denotes	1
T4816	12298-12299	NN	denotes	%
T4817	12299-12300	.	denotes	.
T4818	12300-12488	sentence	denotes	Serial 1:2 dilutions were made with 1× PBS/1% SDS, and 100 μl of each dilution was then vacuum-filtered through a pre-wet 0.22-μm cellulose acetate membrane (Schleicher and Schuell) [22].
T4819	12301-12307	JJ	denotes	Serial
T4821	12308-12309	CD	denotes	1
T4822	12309-12310	SYM	denotes	:
T4823	12310-12311	CD	denotes	2
T4820	12312-12321	NNS	denotes	dilutions
T4825	12322-12326	VBD	denotes	were
T4824	12327-12331	VBN	denotes	made
T4826	12332-12336	IN	denotes	with
T4827	12337-12338	CD	denotes	1
T4829	12338-12339	SYM	denotes	×
T4828	12340-12343	NN	denotes	PBS
T4830	12343-12344	HYPH	denotes	/
T4831	12344-12345	CD	denotes	1
T4832	12345-12346	NN	denotes	%
T4833	12347-12350	NN	denotes	SDS
T4834	12350-12352	,	denotes	,
T4835	12352-12355	CC	denotes	and
T4836	12356-12359	CD	denotes	100
T4837	12360-12362	NN	denotes	μl
T4839	12363-12365	IN	denotes	of
T4840	12366-12370	DT	denotes	each
T4841	12371-12379	NN	denotes	dilution
T4842	12380-12383	VBD	denotes	was
T4843	12384-12388	RB	denotes	then
T4844	12389-12395	NN	denotes	vacuum
T4845	12395-12396	HYPH	denotes	-
T4838	12396-12404	VBN	denotes	filtered
T4846	12405-12412	IN	denotes	through
T4847	12413-12414	DT	denotes	a
T4849	12415-12422	JJ	denotes	pre-wet
T4850	12423-12427	CD	denotes	0.22
T4852	12427-12428	HYPH	denotes	-
T4851	12428-12430	NN	denotes	μm
T4853	12431-12440	NN	denotes	cellulose
T4854	12441-12448	NN	denotes	acetate
T4848	12449-12457	NN	denotes	membrane
T4855	12458-12459	-LRB-	denotes	(
T4856	12459-12469	NNP	denotes	Schleicher
T4857	12470-12473	CC	denotes	and
T4858	12474-12481	NNP	denotes	Schuell
T4859	12481-12482	-RRB-	denotes	)
T4860	12483-12484	-LRB-	denotes	[
T4861	12484-12486	CD	denotes	22
T4862	12486-12487	-RRB-	denotes	]
T4863	12487-12488	.	denotes	.
T4864	12488-12704	sentence	denotes	Each well was washed several times with PBS, after which blots were incubated overnight with polyclonal anti-Aβ antibody (Zymed Laboratories) diluted 1:600 in a blocking solution of 1× TBS/5% nonfat dry milk powder.
T4865	12489-12493	DT	denotes	Each
T4866	12494-12498	NN	denotes	well
T4868	12499-12502	VBD	denotes	was
T4867	12503-12509	VBN	denotes	washed
T4869	12510-12517	JJ	denotes	several
T4870	12518-12523	NNS	denotes	times
T4871	12524-12528	IN	denotes	with
T4872	12529-12532	NN	denotes	PBS
T4873	12532-12534	,	denotes	,
T4874	12534-12539	IN	denotes	after
T4876	12540-12545	WDT	denotes	which
T4877	12546-12551	NNS	denotes	blots
T4878	12552-12556	VBD	denotes	were
T4875	12557-12566	VBN	denotes	incubated
T4879	12567-12576	RB	denotes	overnight
T4880	12577-12581	IN	denotes	with
T4881	12582-12592	JJ	denotes	polyclonal
T4883	12593-12600	JJ	denotes	anti-Aβ
T4882	12601-12609	NN	denotes	antibody
T4884	12610-12611	-LRB-	denotes	(
T4886	12611-12616	NNP	denotes	Zymed
T4885	12617-12629	NNP	denotes	Laboratories
T4887	12629-12630	-RRB-	denotes	)
T4888	12631-12638	VBN	denotes	diluted
T4889	12639-12640	CD	denotes	1
T4890	12640-12641	SYM	denotes	:
T4891	12641-12644	CD	denotes	600
T4892	12645-12647	IN	denotes	in
T4893	12648-12649	DT	denotes	a
T4895	12650-12658	NN	denotes	blocking
T4894	12659-12667	NN	denotes	solution
T4896	12668-12670	IN	denotes	of
T4897	12671-12672	CD	denotes	1
T4899	12672-12673	SYM	denotes	×
T4898	12674-12677	NN	denotes	TBS
T4901	12677-12678	HYPH	denotes	/
T4902	12678-12679	CD	denotes	5
T4903	12679-12680	NN	denotes	%
T4905	12681-12687	JJ	denotes	nonfat
T4906	12688-12691	JJ	denotes	dry
T4904	12692-12696	NN	denotes	milk
T4900	12697-12703	NN	denotes	powder
T4907	12703-12704	.	denotes	.
T4908	12704-12892	sentence	denotes	After washing the blots three times for 10 min each in 1× TBS/0.1% Tween-20, the membrane was incubated for 1 h with HRP-conjugated protein A (Sigma) diluted 1:5,000 in blocking solution.
T4909	12705-12710	IN	denotes	After
T4911	12711-12718	VBG	denotes	washing
T4912	12719-12722	DT	denotes	the
T4913	12723-12728	NNS	denotes	blots
T4914	12729-12734	CD	denotes	three
T4915	12735-12740	NNS	denotes	times
T4916	12741-12744	IN	denotes	for
T4917	12745-12747	CD	denotes	10
T4918	12748-12751	NN	denotes	min
T4919	12752-12756	DT	denotes	each
T4920	12757-12759	IN	denotes	in
T4921	12760-12761	CD	denotes	1
T4923	12761-12762	SYM	denotes	×
T4922	12763-12766	NN	denotes	TBS
T4924	12766-12767	HYPH	denotes	/
T4925	12767-12770	CD	denotes	0.1
T4926	12770-12771	NN	denotes	%
T4927	12772-12777	NN	denotes	Tween
T4928	12777-12778	HYPH	denotes	-
T4929	12778-12780	CD	denotes	20
T4930	12780-12782	,	denotes	,
T4931	12782-12785	DT	denotes	the
T4932	12786-12794	NN	denotes	membrane
T4933	12795-12798	VBD	denotes	was
T4910	12799-12808	VBN	denotes	incubated
T4934	12809-12812	IN	denotes	for
T4935	12813-12814	CD	denotes	1
T4936	12815-12816	NN	denotes	h
T4937	12817-12821	IN	denotes	with
T4938	12822-12825	NN	denotes	HRP
T4940	12825-12826	HYPH	denotes	-
T4939	12826-12836	VBN	denotes	conjugated
T4942	12837-12844	NN	denotes	protein
T4941	12845-12846	NN	denotes	A
T4943	12847-12848	-LRB-	denotes	(
T4944	12848-12853	NNP	denotes	Sigma
T4945	12853-12854	-RRB-	denotes	)
T4946	12855-12862	VBN	denotes	diluted
T4947	12863-12864	CD	denotes	1
T4948	12864-12865	SYM	denotes	:
T4949	12865-12870	CD	denotes	5,000
T4950	12871-12873	IN	denotes	in
T4951	12874-12882	NN	denotes	blocking
T4952	12883-12891	NN	denotes	solution
T4953	12891-12892	.	denotes	.
T4954	12892-13085	sentence	denotes	The membranes were again washed three times with 1× TBS/0.1% Tween-20, before antibody binding was detected with enhanced chemiluminescence (PerkinElmer, Boston, Massachusetts, United States).
T4955	12893-12896	DT	denotes	The
T4956	12897-12906	NNS	denotes	membranes
T4958	12907-12911	VBD	denotes	were
T4959	12912-12917	RB	denotes	again
T4957	12918-12924	VBN	denotes	washed
T4960	12925-12930	CD	denotes	three
T4961	12931-12936	NNS	denotes	times
T4962	12937-12941	IN	denotes	with
T4963	12942-12943	CD	denotes	1
T4965	12943-12944	SYM	denotes	×
T4964	12945-12948	NN	denotes	TBS
T4966	12948-12949	HYPH	denotes	/
T4967	12949-12952	CD	denotes	0.1
T4968	12952-12953	NN	denotes	%
T4969	12954-12959	NN	denotes	Tween
T4970	12959-12960	HYPH	denotes	-
T4971	12960-12962	CD	denotes	20
T4972	12962-12964	,	denotes	,
T4973	12964-12970	IN	denotes	before
T4975	12971-12979	NN	denotes	antibody
T4976	12980-12987	NN	denotes	binding
T4977	12988-12991	VBD	denotes	was
T4974	12992-13000	VBN	denotes	detected
T4978	13001-13005	IN	denotes	with
T4979	13006-13014	VBN	denotes	enhanced
T4980	13015-13032	NN	denotes	chemiluminescence
T4981	13033-13034	-LRB-	denotes	(
T4982	13034-13045	NNP	denotes	PerkinElmer
T4983	13045-13047	,	denotes	,
T4984	13047-13053	NNP	denotes	Boston
T4985	13053-13055	,	denotes	,
T4986	13055-13068	NNP	denotes	Massachusetts
T4987	13068-13070	,	denotes	,
T4988	13070-13076	NNP	denotes	United
T4989	13077-13083	NNP	denotes	States
T4990	13083-13084	-RRB-	denotes	)
T4991	13084-13085	.	denotes	.
T4992	13085-13272	sentence	denotes	Digital images of each blot were captured with a Molecular Imager FX gel documentation system, and the intensity of Aβ staining was quantified using Quantity One image analysis software.
T4993	13086-13093	JJ	denotes	Digital
T4994	13094-13100	NNS	denotes	images
T4996	13101-13103	IN	denotes	of
T4997	13104-13108	DT	denotes	each
T4998	13109-13113	NN	denotes	blot
T4999	13114-13118	VBD	denotes	were
T4995	13119-13127	VBN	denotes	captured
T5000	13128-13132	IN	denotes	with
T5001	13133-13134	DT	denotes	a
T5003	13135-13144	JJ	denotes	Molecular
T5004	13145-13151	NNP	denotes	Imager
T5005	13152-13154	NNP	denotes	FX
T5006	13155-13158	NN	denotes	gel
T5007	13159-13172	NN	denotes	documentation
T5002	13173-13179	NN	denotes	system
T5008	13179-13181	,	denotes	,
T5009	13181-13184	CC	denotes	and
T5010	13185-13188	DT	denotes	the
T5011	13189-13198	NN	denotes	intensity
T5013	13199-13201	IN	denotes	of
T5014	13202-13204	NN	denotes	Aβ
T5015	13205-13213	NN	denotes	staining
T5016	13214-13217	VBD	denotes	was
T5012	13218-13228	VBN	denotes	quantified
T5017	13229-13234	VBG	denotes	using
T5018	13235-13243	NNP	denotes	Quantity
T5020	13244-13247	CD	denotes	One
T5021	13248-13253	NN	denotes	image
T5022	13254-13262	NN	denotes	analysis
T5019	13263-13271	NN	denotes	software
T5023	13271-13272	.	denotes	.
T5075	13274-13276	NN	denotes	Aβ
T5076	13277-13282	NN	denotes	ELISA
T5077	13282-13460	sentence	denotes	An aliquot of cortical homogenate generated for Western analysis described above was subjected to a three-step sequential extraction using PBS, 2% SDS, and 70% formic acid (FA).
T5078	13283-13285	DT	denotes	An
T5079	13286-13293	NN	denotes	aliquot
T5081	13294-13296	IN	denotes	of
T5082	13297-13305	JJ	denotes	cortical
T5083	13306-13316	NN	denotes	homogenate
T5084	13317-13326	VBN	denotes	generated
T5085	13327-13330	IN	denotes	for
T5086	13331-13338	NNP	denotes	Western
T5087	13339-13347	NN	denotes	analysis
T5088	13348-13357	VBN	denotes	described
T5089	13358-13363	RB	denotes	above
T5090	13364-13367	VBD	denotes	was
T5080	13368-13377	VBN	denotes	subjected
T5091	13378-13380	IN	denotes	to
T5092	13381-13382	DT	denotes	a
T5094	13383-13388	CD	denotes	three
T5096	13388-13389	HYPH	denotes	-
T5095	13389-13393	NN	denotes	step
T5097	13394-13404	JJ	denotes	sequential
T5093	13405-13415	NN	denotes	extraction
T5098	13416-13421	VBG	denotes	using
T5099	13422-13425	NN	denotes	PBS
T5100	13425-13427	,	denotes	,
T5101	13427-13428	CD	denotes	2
T5102	13428-13429	NN	denotes	%
T5103	13430-13433	NN	denotes	SDS
T5104	13433-13435	,	denotes	,
T5105	13435-13438	CC	denotes	and
T5106	13439-13441	CD	denotes	70
T5107	13441-13442	NN	denotes	%
T5108	13443-13449	JJ	denotes	formic
T5109	13450-13454	NN	denotes	acid
T5110	13455-13456	-LRB-	denotes	(
T5111	13456-13458	NN	denotes	FA
T5112	13458-13459	-RRB-	denotes	)
T5113	13459-13460	.	denotes	.
T5114	13460-13656	sentence	denotes	At each step, the sample was sonicated in appropriate buffer and centrifuged at 100,000g for 30 min (1- to 1.5-mo samples) or 60 min (6- to 12-mo samples) at 4 °C as previously described [23–25].
T5115	13461-13463	IN	denotes	At
T5117	13464-13468	DT	denotes	each
T5118	13469-13473	NN	denotes	step
T5119	13473-13475	,	denotes	,
T5120	13475-13478	DT	denotes	the
T5121	13479-13485	NN	denotes	sample
T5122	13486-13489	VBD	denotes	was
T5116	13490-13499	VBN	denotes	sonicated
T5123	13500-13502	IN	denotes	in
T5124	13503-13514	JJ	denotes	appropriate
T5125	13515-13521	NN	denotes	buffer
T5126	13522-13525	CC	denotes	and
T5127	13526-13537	VBN	denotes	centrifuged
T5128	13538-13540	IN	denotes	at
T5129	13541-13549	CD	denotes	100,000g
T5130	13550-13553	IN	denotes	for
T5131	13554-13556	CD	denotes	30
T5132	13557-13560	NN	denotes	min
T5133	13561-13562	-LRB-	denotes	(
T5135	13562-13563	CD	denotes	1
T5137	13563-13564	HYPH	denotes	-
T5138	13565-13567	IN	denotes	to
T5136	13568-13571	CD	denotes	1.5
T5140	13571-13572	HYPH	denotes	-
T5139	13572-13574	NN	denotes	mo
T5134	13575-13582	NNS	denotes	samples
T5141	13582-13583	-RRB-	denotes	)
T5142	13584-13586	CC	denotes	or
T5143	13587-13589	CD	denotes	60
T5144	13590-13593	NN	denotes	min
T5145	13594-13595	-LRB-	denotes	(
T5147	13595-13596	CD	denotes	6
T5149	13596-13597	HYPH	denotes	-
T5150	13598-13600	IN	denotes	to
T5148	13601-13603	CD	denotes	12
T5152	13603-13604	HYPH	denotes	-
T5151	13604-13606	NN	denotes	mo
T5146	13607-13614	NNS	denotes	samples
T5153	13614-13615	-RRB-	denotes	)
T5154	13616-13618	IN	denotes	at
T5155	13619-13620	CD	denotes	4
T5156	13621-13623	NN	denotes	°C
T5157	13624-13626	IN	denotes	as
T5159	13627-13637	RB	denotes	previously
T5158	13638-13647	VBN	denotes	described
T5160	13648-13649	-LRB-	denotes	[
T5161	13649-13651	CD	denotes	23
T5162	13651-13652	SYM	denotes	–
T5163	13652-13654	CD	denotes	25
T5164	13654-13655	-RRB-	denotes	]
T5165	13655-13656	.	denotes	.
T5166	13656-13761	sentence	denotes	The supernatant was removed for analysis, and the pellet was sonicated in the next solution in sequence.
T5167	13657-13660	DT	denotes	The
T5168	13661-13672	NN	denotes	supernatant
T5170	13673-13676	VBD	denotes	was
T5169	13677-13684	VBN	denotes	removed
T5171	13685-13688	IN	denotes	for
T5172	13689-13697	NN	denotes	analysis
T5173	13697-13699	,	denotes	,
T5174	13699-13702	CC	denotes	and
T5175	13703-13706	DT	denotes	the
T5176	13707-13713	NN	denotes	pellet
T5178	13714-13717	VBD	denotes	was
T5177	13718-13727	VBN	denotes	sonicated
T5179	13728-13730	IN	denotes	in
T5180	13731-13734	DT	denotes	the
T5182	13735-13739	JJ	denotes	next
T5181	13740-13748	NN	denotes	solution
T5183	13749-13751	IN	denotes	in
T5184	13752-13760	NN	denotes	sequence
T5185	13760-13761	.	denotes	.
T5186	13761-13924	sentence	denotes	The 2% SDS extracts were diluted in EC buffer, and the FA extracts neutralized with 1M Tris-phosphate buffer (pH 11) then diluted with EC buffer prior to testing.
T5187	13762-13765	DT	denotes	The
T5189	13766-13767	CD	denotes	2
T5190	13767-13768	NN	denotes	%
T5191	13769-13772	NN	denotes	SDS
T5188	13773-13781	NNS	denotes	extracts
T5193	13782-13786	VBD	denotes	were
T5192	13787-13794	VBN	denotes	diluted
T5194	13795-13797	IN	denotes	in
T5195	13798-13800	NN	denotes	EC
T5196	13801-13807	NN	denotes	buffer
T5197	13807-13809	,	denotes	,
T5198	13809-13812	CC	denotes	and
T5199	13813-13816	DT	denotes	the
T5201	13817-13819	NN	denotes	FA
T5200	13820-13828	NNS	denotes	extracts
T5202	13829-13840	VBN	denotes	neutralized
T5203	13841-13845	IN	denotes	with
T5204	13846-13848	NN	denotes	1M
T5206	13849-13853	NN	denotes	Tris
T5207	13853-13854	HYPH	denotes	-
T5208	13854-13863	NN	denotes	phosphate
T5205	13864-13870	NN	denotes	buffer
T5209	13871-13872	-LRB-	denotes	(
T5210	13872-13874	NN	denotes	pH
T5211	13875-13877	CD	denotes	11
T5212	13877-13878	-RRB-	denotes	)
T5213	13879-13883	RB	denotes	then
T5214	13884-13891	VBD	denotes	diluted
T5215	13892-13896	IN	denotes	with
T5216	13897-13899	NN	denotes	EC
T5217	13900-13906	NN	denotes	buffer
T5218	13907-13912	JJ	denotes	prior
T5219	13913-13915	IN	denotes	to
T5220	13916-13923	NN	denotes	testing
T5221	13923-13924	.	denotes	.
T5222	13924-14110	sentence	denotes	Human Aβ was measured in each fraction using BAN50 for capture (epitope Aβ1–16) and BA27 and BC05 for detection (Aβ40 and Aβ42, respectively) (Takeda Chemical Industries, Osaka, Japan).
T5223	13925-13930	JJ	denotes	Human
T5224	13931-13933	NN	denotes	Aβ
T5226	13934-13937	VBD	denotes	was
T5225	13938-13946	VBN	denotes	measured
T5227	13947-13949	IN	denotes	in
T5228	13950-13954	DT	denotes	each
T5229	13955-13963	NN	denotes	fraction
T5230	13964-13969	VBG	denotes	using
T5231	13970-13975	NN	denotes	BAN50
T5232	13976-13979	IN	denotes	for
T5233	13980-13987	NN	denotes	capture
T5234	13988-13989	-LRB-	denotes	(
T5236	13989-13996	NN	denotes	epitope
T5237	13997-14000	NN	denotes	Aβ1
T5238	14000-14001	HYPH	denotes	–
T5235	14001-14003	NN	denotes	16
T5239	14003-14004	-RRB-	denotes	)
T5240	14005-14008	CC	denotes	and
T5241	14009-14013	NN	denotes	BA27
T5242	14014-14017	CC	denotes	and
T5243	14018-14022	NN	denotes	BC05
T5244	14023-14026	IN	denotes	for
T5245	14027-14036	NN	denotes	detection
T5246	14037-14038	-LRB-	denotes	(
T5247	14038-14042	NN	denotes	Aβ40
T5248	14043-14046	CC	denotes	and
T5249	14047-14051	NN	denotes	Aβ42
T5250	14051-14053	,	denotes	,
T5251	14053-14065	RB	denotes	respectively
T5252	14065-14066	-RRB-	denotes	)
T5253	14067-14068	-LRB-	denotes	(
T5255	14068-14074	NNP	denotes	Takeda
T5256	14075-14083	NNP	denotes	Chemical
T5254	14084-14094	NNPS	denotes	Industries
T5257	14094-14096	,	denotes	,
T5258	14096-14101	NNP	denotes	Osaka
T5259	14101-14103	,	denotes	,
T5260	14103-14108	NNP	denotes	Japan
T5261	14108-14109	-RRB-	denotes	)
T5262	14109-14110	.	denotes	.
T5263	14110-14267	sentence	denotes	Total Aβ (mouse + human; 1- to 1.5-mo samples only) was measured in each fraction using BNT77 for capture (epitope Aβ11–28) and BA27 and BC05 for detection.
T5264	14111-14116	JJ	denotes	Total
T5265	14117-14119	NN	denotes	Aβ
T5267	14120-14121	-LRB-	denotes	(
T5269	14121-14126	NN	denotes	mouse
T5270	14127-14128	SYM	denotes	+
T5271	14129-14134	JJ	denotes	human
T5272	14134-14135	:	denotes	;
T5273	14136-14137	CD	denotes	1
T5275	14137-14138	HYPH	denotes	-
T5276	14139-14141	IN	denotes	to
T5274	14142-14145	CD	denotes	1.5
T5278	14145-14146	HYPH	denotes	-
T5277	14146-14148	NN	denotes	mo
T5268	14149-14156	NNS	denotes	samples
T5279	14157-14161	RB	denotes	only
T5280	14161-14162	-RRB-	denotes	)
T5281	14163-14166	VBD	denotes	was
T5266	14167-14175	VBN	denotes	measured
T5282	14176-14178	IN	denotes	in
T5283	14179-14183	DT	denotes	each
T5284	14184-14192	NN	denotes	fraction
T5285	14193-14198	VBG	denotes	using
T5286	14199-14204	NN	denotes	BNT77
T5287	14205-14208	IN	denotes	for
T5288	14209-14216	NN	denotes	capture
T5289	14217-14218	-LRB-	denotes	(
T5291	14218-14225	NN	denotes	epitope
T5290	14226-14230	NN	denotes	Aβ11
T5292	14230-14231	HYPH	denotes	–
T5293	14231-14233	CD	denotes	28
T5294	14233-14234	-RRB-	denotes	)
T5295	14235-14238	CC	denotes	and
T5296	14239-14243	NN	denotes	BA27
T5297	14244-14247	CC	denotes	and
T5298	14248-14252	NN	denotes	BC05
T5299	14253-14256	IN	denotes	for
T5300	14257-14266	NN	denotes	detection
T5301	14266-14267	.	denotes	.
T5302	14267-14364	sentence	denotes	All values were calculated as picomoles per gram based on the initial weight of cortical tissue.
T5303	14268-14271	DT	denotes	All
T5304	14272-14278	NNS	denotes	values
T5306	14279-14283	VBD	denotes	were
T5305	14284-14294	VBN	denotes	calculated
T5307	14295-14297	IN	denotes	as
T5308	14298-14307	NNS	denotes	picomoles
T5309	14308-14311	IN	denotes	per
T5310	14312-14316	NN	denotes	gram
T5311	14317-14322	VBN	denotes	based
T5312	14323-14325	IN	denotes	on
T5313	14326-14329	DT	denotes	the
T5315	14330-14337	JJ	denotes	initial
T5314	14338-14344	NN	denotes	weight
T5316	14345-14347	IN	denotes	of
T5317	14348-14356	JJ	denotes	cortical
T5318	14357-14363	NN	denotes	tissue
T5319	14363-14364	.	denotes	.
T5339	14366-14374	NN	denotes	Activity
T5340	14375-14385	NN	denotes	Monitoring
T5341	14385-14494	sentence	denotes	Daily basal activity was studied in 28 CaMKIIα-tTA × tet-APPswe/ind line 107 mice between 4 and 5 mo of age.
T5342	14386-14391	JJ	denotes	Daily
T5344	14392-14397	JJ	denotes	basal
T5343	14398-14406	NN	denotes	activity
T5346	14407-14410	VBD	denotes	was
T5345	14411-14418	VBN	denotes	studied
T5347	14419-14421	IN	denotes	in
T5348	14422-14424	CD	denotes	28
T5350	14425-14432	NN	denotes	CaMKIIα
T5352	14432-14433	HYPH	denotes	-
T5353	14433-14436	NN	denotes	tTA
T5354	14437-14438	SYM	denotes	×
T5355	14439-14442	NN	denotes	tet
T5356	14442-14443	HYPH	denotes	-
T5357	14443-14449	NN	denotes	APPswe
T5358	14449-14450	HYPH	denotes	/
T5351	14450-14453	NN	denotes	ind
T5359	14454-14458	NN	denotes	line
T5360	14459-14462	CD	denotes	107
T5349	14463-14467	NNS	denotes	mice
T5361	14468-14475	IN	denotes	between
T5362	14476-14477	CD	denotes	4
T5364	14478-14481	CC	denotes	and
T5365	14482-14483	CD	denotes	5
T5363	14484-14486	NN	denotes	mo
T5366	14487-14489	IN	denotes	of
T5367	14490-14493	NN	denotes	age
T5368	14493-14494	.	denotes	.
T5369	14494-14650	sentence	denotes	Animals were separated into individual cages immediately before the start of each experiment (n = 3–6 per genotype untreated, 2–5 per genotype dox-reared).
T5370	14495-14502	NNS	denotes	Animals
T5372	14503-14507	VBD	denotes	were
T5371	14508-14517	VBN	denotes	separated
T5373	14518-14522	IN	denotes	into
T5374	14523-14533	JJ	denotes	individual
T5375	14534-14539	NNS	denotes	cages
T5376	14540-14551	RB	denotes	immediately
T5377	14552-14558	IN	denotes	before
T5378	14559-14562	DT	denotes	the
T5379	14563-14568	NN	denotes	start
T5380	14569-14571	IN	denotes	of
T5381	14572-14576	DT	denotes	each
T5382	14577-14587	NN	denotes	experiment
T5383	14588-14589	-LRB-	denotes	(
T5385	14589-14590	NN	denotes	n
T5386	14591-14592	SYM	denotes	=
T5387	14593-14594	CD	denotes	3
T5388	14594-14595	SYM	denotes	–
T5384	14595-14596	CD	denotes	6
T5389	14597-14600	IN	denotes	per
T5390	14601-14609	NN	denotes	genotype
T5391	14610-14619	JJ	denotes	untreated
T5392	14619-14621	,	denotes	,
T5393	14621-14622	CD	denotes	2
T5395	14622-14623	SYM	denotes	–
T5394	14623-14624	CD	denotes	5
T5396	14625-14628	IN	denotes	per
T5397	14629-14637	NN	denotes	genotype
T5398	14638-14641	NN	denotes	dox
T5400	14641-14642	HYPH	denotes	-
T5399	14642-14648	VBN	denotes	reared
T5401	14648-14649	-RRB-	denotes	)
T5402	14649-14650	.	denotes	.
T5403	14650-14879	sentence	denotes	The cages were placed inside activity-monitoring frames designed to count every time the animal passed through one of three photobeams spanning the width of the cage (San Diego Instruments, San Diego, California, United States).
T5404	14651-14654	DT	denotes	The
T5405	14655-14660	NNS	denotes	cages
T5407	14661-14665	VBD	denotes	were
T5406	14666-14672	VBN	denotes	placed
T5408	14673-14679	IN	denotes	inside
T5409	14680-14688	NN	denotes	activity
T5411	14688-14689	HYPH	denotes	-
T5410	14689-14699	VBG	denotes	monitoring
T5412	14700-14706	NNS	denotes	frames
T5413	14707-14715	VBN	denotes	designed
T5414	14716-14718	TO	denotes	to
T5415	14719-14724	VB	denotes	count
T5416	14725-14730	DT	denotes	every
T5417	14731-14735	NN	denotes	time
T5418	14736-14739	DT	denotes	the
T5419	14740-14746	NN	denotes	animal
T5420	14747-14753	VBN	denotes	passed
T5421	14754-14761	IN	denotes	through
T5422	14762-14765	CD	denotes	one
T5423	14766-14768	IN	denotes	of
T5424	14769-14774	CD	denotes	three
T5425	14775-14785	NNS	denotes	photobeams
T5426	14786-14794	VBG	denotes	spanning
T5427	14795-14798	DT	denotes	the
T5428	14799-14804	NN	denotes	width
T5429	14805-14807	IN	denotes	of
T5430	14808-14811	DT	denotes	the
T5431	14812-14816	NN	denotes	cage
T5432	14817-14818	-LRB-	denotes	(
T5434	14818-14821	NNP	denotes	San
T5435	14822-14827	NNP	denotes	Diego
T5433	14828-14839	NNP	denotes	Instruments
T5436	14839-14841	,	denotes	,
T5437	14841-14844	NNP	denotes	San
T5438	14845-14850	NNP	denotes	Diego
T5439	14850-14852	,	denotes	,
T5440	14852-14862	NNP	denotes	California
T5441	14862-14864	,	denotes	,
T5442	14864-14870	NNP	denotes	United
T5443	14871-14877	NNP	denotes	States
T5444	14877-14878	-RRB-	denotes	)
T5445	14878-14879	.	denotes	.
T5446	14879-15007	sentence	denotes	Experiments were started midway through the light phase of the day, and data were collected in 1-h bins for the following 48 h.
T5447	14880-14891	NNS	denotes	Experiments
T5449	14892-14896	VBD	denotes	were
T5448	14897-14904	VBN	denotes	started
T5450	14905-14911	RB	denotes	midway
T5451	14912-14919	IN	denotes	through
T5452	14920-14923	DT	denotes	the
T5454	14924-14929	NN	denotes	light
T5453	14930-14935	NN	denotes	phase
T5455	14936-14938	IN	denotes	of
T5456	14939-14942	DT	denotes	the
T5457	14943-14946	NN	denotes	day
T5458	14946-14948	,	denotes	,
T5459	14948-14951	CC	denotes	and
T5460	14952-14956	NNS	denotes	data
T5462	14957-14961	VBD	denotes	were
T5461	14962-14971	VBN	denotes	collected
T5463	14972-14974	IN	denotes	in
T5464	14975-14976	CD	denotes	1
T5466	14976-14977	HYPH	denotes	-
T5465	14977-14978	NN	denotes	h
T5467	14979-14983	NNS	denotes	bins
T5468	14984-14987	IN	denotes	for
T5469	14988-14991	DT	denotes	the
T5471	14992-15001	JJ	denotes	following
T5472	15002-15004	CD	denotes	48
T5470	15005-15006	NN	denotes	h
T5473	15006-15007	.	denotes	.
T5474	15007-15154	sentence	denotes	Testing rooms were maintained on the same 13:11 h day:night cycle as the main animal housing areas and were closed to entry during the experiment.
T5475	15008-15015	NN	denotes	Testing
T5476	15016-15021	NNS	denotes	rooms
T5478	15022-15026	VBD	denotes	were
T5477	15027-15037	VBN	denotes	maintained
T5479	15038-15040	IN	denotes	on
T5480	15041-15044	DT	denotes	the
T5482	15045-15049	JJ	denotes	same
T5483	15050-15052	CD	denotes	13
T5485	15052-15053	SYM	denotes	:
T5486	15053-15055	CD	denotes	11
T5484	15056-15057	NN	denotes	h
T5487	15058-15061	NN	denotes	day
T5488	15061-15062	SYM	denotes	:
T5489	15062-15067	NN	denotes	night
T5481	15068-15073	NN	denotes	cycle
T5490	15074-15076	IN	denotes	as
T5491	15077-15080	DT	denotes	the
T5493	15081-15085	JJ	denotes	main
T5494	15086-15092	NN	denotes	animal
T5495	15093-15100	NN	denotes	housing
T5492	15101-15106	NNS	denotes	areas
T5496	15107-15110	CC	denotes	and
T5497	15111-15115	VBD	denotes	were
T5498	15116-15122	VBN	denotes	closed
T5499	15123-15125	IN	denotes	to
T5500	15126-15131	NN	denotes	entry
T5501	15132-15138	IN	denotes	during
T5502	15139-15142	DT	denotes	the
T5503	15143-15153	NN	denotes	experiment
T5504	15153-15154	.	denotes	.
T5510	15156-15167	JJ	denotes	Statistical
T5511	15168-15176	NNS	denotes	Analyses
T5512	15176-15448	sentence	denotes	Statistical analyses of protein expression, ELISA data, and filter trap assays were performed by ANOVA with Tukey's honest significant difference post-hoc test applied to significant main effects or interactions (Statistica 6.0, StatSoft, Tulsa, Oklahoma, United States).
T5513	15177-15188	JJ	denotes	Statistical
T5514	15189-15197	NNS	denotes	analyses
T5516	15198-15200	IN	denotes	of
T5517	15201-15208	NN	denotes	protein
T5518	15209-15219	NN	denotes	expression
T5519	15219-15221	,	denotes	,
T5520	15221-15226	NN	denotes	ELISA
T5521	15227-15231	NNS	denotes	data
T5522	15231-15233	,	denotes	,
T5523	15233-15236	CC	denotes	and
T5524	15237-15243	NN	denotes	filter
T5525	15244-15248	NN	denotes	trap
T5526	15249-15255	NNS	denotes	assays
T5527	15256-15260	VBD	denotes	were
T5515	15261-15270	VBN	denotes	performed
T5528	15271-15273	IN	denotes	by
T5529	15274-15279	NN	denotes	ANOVA
T5530	15280-15284	IN	denotes	with
T5531	15285-15290	NNP	denotes	Tukey
T5533	15290-15292	POS	denotes	's
T5534	15293-15299	JJ	denotes	honest
T5535	15300-15311	JJ	denotes	significant
T5536	15312-15322	NN	denotes	difference
T5537	15323-15327	FW	denotes	post
T5539	15327-15328	HYPH	denotes	-
T5538	15328-15331	FW	denotes	hoc
T5532	15332-15336	NN	denotes	test
T5540	15337-15344	VBN	denotes	applied
T5541	15345-15347	IN	denotes	to
T5542	15348-15359	JJ	denotes	significant
T5544	15360-15364	JJ	denotes	main
T5543	15365-15372	NNS	denotes	effects
T5545	15373-15375	CC	denotes	or
T5546	15376-15388	NNS	denotes	interactions
T5547	15389-15390	-LRB-	denotes	(
T5548	15390-15400	NNP	denotes	Statistica
T5549	15401-15404	CD	denotes	6.0
T5550	15404-15406	,	denotes	,
T5551	15406-15414	NNP	denotes	StatSoft
T5552	15414-15416	,	denotes	,
T5553	15416-15421	NNP	denotes	Tulsa
T5554	15421-15423	,	denotes	,
T5555	15423-15431	NNP	denotes	Oklahoma
T5556	15431-15433	,	denotes	,
T5557	15433-15439	NNP	denotes	United
T5558	15440-15446	NNP	denotes	States
T5559	15446-15447	-RRB-	denotes	)
T5560	15447-15448	.	denotes	.
T5561	15448-15588	sentence	denotes	In cases of positively skewed data distribution, log10(x + 0.5) transformation was applied to the raw data before submitting them to ANOVA.
T5562	15449-15451	IN	denotes	In
T5564	15452-15457	NNS	denotes	cases
T5565	15458-15460	IN	denotes	of
T5566	15461-15471	RB	denotes	positively
T5567	15472-15478	VBN	denotes	skewed
T5569	15479-15483	NNS	denotes	data
T5568	15484-15496	NN	denotes	distribution
T5570	15496-15498	,	denotes	,
T5571	15498-15503	NN	denotes	log10
T5573	15503-15504	-LRB-	denotes	(
T5572	15504-15505	NN	denotes	x
T5575	15506-15507	SYM	denotes	+
T5576	15508-15511	CD	denotes	0.5
T5577	15511-15512	-RRB-	denotes	)
T5574	15513-15527	NN	denotes	transformation
T5578	15528-15531	VBD	denotes	was
T5563	15532-15539	VBN	denotes	applied
T5579	15540-15542	IN	denotes	to
T5580	15543-15546	DT	denotes	the
T5582	15547-15550	JJ	denotes	raw
T5581	15551-15555	NNS	denotes	data
T5583	15556-15562	IN	denotes	before
T5584	15563-15573	VBG	denotes	submitting
T5585	15574-15578	PRP	denotes	them
T5586	15579-15581	IN	denotes	to
T5587	15582-15587	NN	denotes	ANOVA
T5588	15587-15588	.	denotes	.
R1000	T1625	T1626	det	the,promoter
R1001	T1626	T1624	dobj	promoter,cloning
R1002	T1627	T1628	npadvmod	tetracycline,responsive
R1003	T1628	T1626	amod	responsive,promoter
R1004	T1629	T1628	punct	-,responsive
R1005	T1630	T1631	punct	(,6
R1006	T1631	T1626	parataxis	6,promoter
R1007	T1632	T1631	nmod	bp,6
R1008	T1633	T1634	punct	–,481
R1009	T1634	T1631	prep	481,6
R1010	T1635	T1631	punct	),6
R1011	T1636	T1624	prep	from,cloning
R1012	T1637	T1636	pobj	pTetSplice,from
R1013	T1638	T1637	prep	by,pTetSplice
R1014	T1639	T1638	pobj	PCR,by
R1015	T1640	T1639	acl	using,PCR
R1016	T1641	T1640	dobj	primers,using
R1017	T1642	T1643	dep	that,added
R1018	T1643	T1641	relcl	added,primers
R1019	T1644	T1645	amod	external,sites
R1020	T1645	T1643	dobj	sites,added
R1021	T1646	T1645	nmod	BamHI,sites
R1022	T1647	T1646	cc	and,BamHI
R1023	T1648	T1646	conj	NotI,BamHI
R1024	T1649	T1643	prep	to,added
R1025	T1650	T1651	det	the,end
R1026	T1651	T1649	pobj	end,to
R1027	T1652	T1651	nummod	5,end
R1028	T1653	T1652	punct	′,5
R1029	T1654	T1643	cc	and,added
R1030	T1655	T1656	det	a,site
R1031	T1656	T1643	conj	site,added
R1032	T1657	T1656	compound	BamHI,site
R1033	T1658	T1656	prep	to,site
R1034	T1659	T1660	det	the,end
R1035	T1660	T1658	pobj	end,to
R1036	T1661	T1660	nummod	3,end
R1037	T1662	T1661	punct	′,3
R1038	T1663	T1622	punct	", ",began
R1039	T1664	T1665	mark	while,destroying
R1040	T1665	T1622	advcl	destroying,began
R1041	T1666	T1667	nmod	XhoI,sites
R1042	T1667	T1665	dobj	sites,destroying
R1043	T1668	T1666	cc	and,XhoI
R1044	T1669	T1666	conj	BamHI,XhoI
R1045	T1670	T1667	prep	within,sites
R1046	T1671	T1672	det	the,promoter
R1047	T1672	T1670	pobj	promoter,within
R1048	T1673	T1674	punct	(,CCG
R1049	T1674	T1665	parataxis	CCG,destroying
R1050	T1675	T1676	dep	forward,C
R1051	T1676	T1674	dep	C,CCG
R1052	T1677	T1676	punct	: ,C
R1053	T1678	T1676	compound	GCC,C
R1054	T1679	T1676	compound	GGA,C
R1055	T1680	T1676	compound	TCC,C
R1056	T1681	T1676	compound	GCG,C
R1057	T1682	T1676	compound	GCC,C
R1058	T1683	T1676	compound	GCC,C
R1059	T1684	T1676	compound	GTC,C
R1060	T1685	T1676	compound	GAG,C
R1061	T1686	T1676	compound	TTT,C
R1062	T1687	T1676	compound	ACC,C
R1063	T1688	T1676	compound	ACT,C
R1064	T1689	T1676	compound	CCC,C
R1065	T1690	T1676	compound	TAT,C
R1066	T1691	T1674	punct	;,CCG
R1067	T1692	T1674	dep	reverse,CCG
R1068	T1693	T1674	punct	: ,CCG
R1069	T1694	T1674	compound	GCC,CCG
R1070	T1695	T1674	compound	GGA,CCG
R1071	T1696	T1674	compound	TCC,CCG
R1072	T1697	T1674	compound	ACT,CCG
R1073	T1698	T1674	compound	CTA,CCG
R1074	T1699	T1674	compound	GAA,CCG
R1075	T1700	T1674	compound	GAT,CCG
R1076	T1701	T1674	compound	CCC,CCG
R1077	T1702	T1674	compound	CGG,CCG
R1078	T1703	T1674	compound	GTA,CCG
R1079	T1704	T1674	punct	),CCG
R1080	T1705	T1622	punct	.,began
R1081	T1707	T1708	nsubj	We,isolated
R1082	T1709	T1708	advmod	then,isolated
R1083	T1710	T1711	det	the,intron
R1084	T1711	T1708	dobj	intron,isolated
R1085	T1712	T1711	compound	moPrP.XhoI,intron
R1086	T1713	T1708	prep	by,isolated
R1087	T1714	T1713	pobj	amplification,by
R1088	T1715	T1714	prep	with,amplification
R1089	T1716	T1715	pobj	primers,with
R1090	T1717	T1718	dep	that,added
R1091	T1718	T1716	relcl	added,primers
R1092	T1719	T1720	det	an,site
R1093	T1720	T1718	dobj	site,added
R1094	T1721	T1720	amod	external,site
R1095	T1722	T1720	compound	BamHI,site
R1096	T1723	T1718	prep	to,added
R1097	T1724	T1725	det	the,end
R1098	T1725	T1723	pobj	end,to
R1099	T1726	T1725	nummod	5,end
R1100	T1727	T1726	punct	′,5
R1101	T1728	T1725	prep	of,end
R1102	T1729	T1728	pobj	exon,of
R1103	T1730	T1729	nummod	1,exon
R1104	T1731	T1718	cc	and,added
R1105	T1732	T1718	conj	ran,added
R1106	T1733	T1732	prep	through,ran
R1107	T1734	T1735	det	the,site
R1108	T1735	T1733	pobj	site,through
R1109	T1736	T1735	compound	Asp718,site
R1110	T1737	T1735	prep	of,site
R1111	T1738	T1737	pobj	exon,of
R1112	T1739	T1738	nummod	2,exon
R1113	T1740	T1741	punct	(,TGC
R1114	T1741	T1735	parataxis	TGC,site
R1115	T1742	T1743	dep	forward,GG
R1116	T1743	T1741	dep	GG,TGC
R1117	T1744	T1743	punct	: ,GG
R1118	T1745	T1743	compound	GCC,GG
R1119	T1746	T1743	compound	GGA,GG
R1120	T1747	T1743	compound	TCC,GG
R1121	T1748	T1743	compound	GAT,GG
R1122	T1749	T1743	compound	CAG,GG
R1123	T1750	T1743	compound	CAG,GG
R1124	T1751	T1743	compound	ACC,GG
R1125	T1752	T1743	compound	GAT,GG
R1126	T1753	T1743	compound	TCT,GG
R1127	T1754	T1741	punct	;,TGC
R1128	T1755	T1741	dep	reverse,TGC
R1129	T1756	T1741	punct	: ,TGC
R1130	T1757	T1741	compound	GCC,TGC
R1131	T1758	T1741	compound	GGT,TGC
R1132	T1759	T1741	compound	ACC,TGC
R1133	T1760	T1741	compound	ACT,TGC
R1134	T1761	T1741	compound	AGG,TGC
R1135	T1762	T1741	compound	AAG,TGC
R1136	T1763	T1741	compound	GCA,TGC
R1137	T1764	T1741	compound	GAA,TGC
R1138	T1765	T1741	punct	),TGC
R1139	T1766	T1708	punct	.,isolated
R1140	T1768	T1769	det	This,fragment
R1141	T1769	T1774	nsubj	fragment,cloned
R1142	T1770	T1771	nummod	2,kb
R1143	T1771	T1769	compound	kb,fragment
R1144	T1772	T1771	punct	-,kb
R1145	T1773	T1769	compound	intron,fragment
R1146	T1775	T1774	aux	was,cloned
R1147	T1776	T1774	prep	into,cloned
R1148	T1777	T1778	det	a,vector
R1149	T1778	T1776	pobj	vector,into
R1150	T1779	T1780	compound	TA,cloning
R1151	T1780	T1778	compound	cloning,vector
R1152	T1781	T1782	punct	(,Invitrogen
R1153	T1782	T1778	parataxis	Invitrogen,vector
R1154	T1783	T1782	punct	", ",Invitrogen
R1155	T1784	T1782	npadvmod	Carlsbad,Invitrogen
R1156	T1785	T1782	punct	", ",Invitrogen
R1157	T1786	T1782	npadvmod	California,Invitrogen
R1158	T1787	T1782	punct	", ",Invitrogen
R1159	T1788	T1789	compound	United,States
R1160	T1789	T1782	npadvmod	States,Invitrogen
R1161	T1790	T1782	punct	),Invitrogen
R1162	T1791	T1774	punct	", ",cloned
R1163	T1792	T1793	advmod	then,excised
R1164	T1793	T1774	conj	excised,cloned
R1165	T1794	T1793	prep	by,excised
R1166	T1795	T1796	compound	Asp718,digestion
R1167	T1796	T1794	pobj	digestion,by
R1168	T1797	T1793	cc	and,excised
R1169	T1798	T1793	conj	ligated,excised
R1170	T1799	T1798	prep	to,ligated
R1171	T1800	T1801	det	the,fragment
R1172	T1801	T1799	pobj	fragment,to
R1173	T1802	T1803	nummod	6.8,kb
R1174	T1803	T1801	compound	kb,fragment
R1175	T1804	T1803	punct	-,kb
R1176	T1805	T1801	compound	Asp718,fragment
R1177	T1806	T1801	prep	of,fragment
R1178	T1807	T1806	pobj	moPrP.XhoI,of
R1179	T1808	T1801	acl	containing,fragment
R1180	T1809	T1808	dobj	exon,containing
R1181	T1810	T1809	nummod	2,exon
R1182	T1811	T1809	punct	", ",exon
R1183	T1812	T1809	conj	exon,exon
R1184	T1813	T1812	nummod	3,exon
R1185	T1814	T1812	punct	", ",exon
R1186	T1815	T1816	det	the,UTR
R1187	T1816	T1812	conj	UTR,exon
R1188	T1817	T1816	nummod	3,UTR
R1189	T1818	T1817	punct	′,3
R1190	T1819	T1816	punct	", ",UTR
R1191	T1820	T1816	cc	and,UTR
R1192	T1821	T1816	conj	pBluescript,UTR
R1193	T1822	T1823	aux	to,generate
R1194	T1823	T1774	advcl	generate,cloned
R1195	T1824	T1825	det	an,vector
R1196	T1825	T1823	dobj	vector,generate
R1197	T1826	T1825	amod	intermediate,vector
R1198	T1827	T1823	prep	with,generate
R1199	T1828	T1829	det	all,exons
R1200	T1829	T1827	pobj	exons,with
R1201	T1830	T1829	nummod	three,exons
R1202	T1831	T1829	cc	and,exons
R1203	T1832	T1833	det	a,intron
R1204	T1833	T1829	conj	intron,exons
R1205	T1834	T1833	amod	central,intron
R1206	T1835	T1829	cc	but,exons
R1207	T1836	T1837	det	no,promoter
R1208	T1837	T1829	conj	promoter,exons
R1209	T1838	T1774	punct	.,cloned
R1210	T1840	T1841	det	This,vector
R1211	T1841	T1842	nsubjpass	vector,opened
R1212	T1843	T1842	auxpass	was,opened
R1213	T1844	T1842	advmod	then,opened
R1214	T1845	T1842	prep	at,opened
R1215	T1846	T1847	det	the,site
R1216	T1847	T1845	pobj	site,at
R1217	T1848	T1847	compound	BamHI,site
R1218	T1849	T1847	acl	introduced,site
R1219	T1850	T1849	agent	by,introduced
R1220	T1851	T1852	det	the,primer
R1221	T1852	T1850	pobj	primer,by
R1222	T1853	T1852	compound	intron,primer
R1223	T1854	T1852	compound	cloning,primer
R1224	T1855	T1842	punct	", ",opened
R1225	T1856	T1842	cc	and,opened
R1226	T1857	T1842	conj	ligated,opened
R1227	T1858	T1857	prep	to,ligated
R1228	T1859	T1860	det	the,fragment
R1229	T1860	T1858	pobj	fragment,to
R1230	T1861	T1862	nummod	0.5,kb
R1231	T1862	T1860	compound	kb,fragment
R1232	T1863	T1862	punct	-,kb
R1233	T1864	T1865	compound	BamHI,cut
R1234	T1865	T1860	compound	cut,fragment
R1235	T1866	T1865	punct	-,cut
R1236	T1867	T1868	compound	tetracycline,promoter
R1237	T1868	T1860	compound	promoter,fragment
R1238	T1869	T1842	punct	.,opened
R1239	T1871	T1872	det	This,ligation
R1240	T1872	T1873	nsubj	ligation,generated
R1241	T1874	T1875	det	a,vector
R1242	T1875	T1873	dobj	vector,generated
R1243	T1876	T1877	nummod	9.3,kb
R1244	T1877	T1875	compound	kb,vector
R1245	T1878	T1877	punct	-,kb
R1246	T1879	T1875	acl	encoding,vector
R1247	T1880	T1881	det	the,promoter
R1248	T1881	T1879	dobj	promoter,encoding
R1249	T1882	T1881	compound	tetracycline,promoter
R1250	T1883	T1881	prep	from,promoter
R1251	T1884	T1883	pobj	pTetSplice,from
R1252	T1885	T1879	prep	with,encoding
R1253	T1886	T1887	nummod	two,exons
R1254	T1887	T1885	pobj	exons,with
R1255	T1888	T1887	punct	", ",exons
R1256	T1889	T1890	nummod	one,intron
R1257	T1890	T1887	conj	intron,exons
R1258	T1891	T1890	punct	", ",intron
R1259	T1892	T1890	cc	and,intron
R1260	T1893	T1894	det	the,UTR
R1261	T1894	T1890	conj	UTR,intron
R1262	T1895	T1894	amod	original,UTR
R1263	T1896	T1894	nummod	3,UTR
R1264	T1897	T1896	punct	′,3
R1265	T1898	T1894	prep	of,UTR
R1266	T1899	T1900	det	the,vector
R1267	T1900	T1898	pobj	vector,of
R1268	T1901	T1900	compound	moPrP.XhoI,vector
R1269	T1902	T1887	punct	", ",exons
R1270	T1903	T1904	advmod	all,carried
R1271	T1904	T1887	acl	carried,exons
R1272	T1905	T1904	prep	in,carried
R1273	T1906	T1907	det	the,vector
R1274	T1907	T1905	pobj	vector,in
R1275	T1908	T1909	compound	pBluescript,cloning
R1276	T1909	T1907	compound	cloning,vector
R1277	T1910	T1873	punct	.,generated
R1278	T1912	T1913	nsubj	We,incorporated
R1279	T1913	T1914	ccomp	incorporated,generated
R1280	T1915	T1916	det	the,mutations
R1281	T1916	T1913	dobj	mutations,incorporated
R1282	T1917	T1916	amod	Swedish,mutations
R1283	T1918	T1919	punct	(,571NL
R1284	T1919	T1917	parataxis	571NL,Swedish
R1285	T1920	T1919	compound	KM570,571NL
R1286	T1921	T1919	punct	/,571NL
R1287	T1922	T1919	punct	),571NL
R1288	T1923	T1917	cc	and,Swedish
R1289	T1924	T1917	conj	Indiana,Swedish
R1290	T1925	T1926	punct	(,V617F
R1291	T1926	T1924	parataxis	V617F,Indiana
R1292	T1927	T1926	punct	),V617F
R1293	T1928	T1913	prep	into,incorporated
R1294	T1929	T1930	det	the,cDNA
R1295	T1930	T1928	pobj	cDNA,into
R1296	T1931	T1932	compound	mo,huAPP695
R1297	T1932	T1930	compound	huAPP695,cDNA
R1298	T1933	T1932	punct	/,huAPP695
R1299	T1934	T1930	punct	(,cDNA
R1300	T1935	T1930	prep	in,cDNA
R1301	T1936	T1937	compound	BS,KS
R1302	T1937	T1935	pobj	KS,in
R1303	T1938	T1937	punct	-,KS
R1304	T1939	T1913	punct	),incorporated
R1305	T1940	T1913	prep	by,incorporated
R1306	T1941	T1940	pobj	PCR,by
R1307	T1942	T1913	advcl	using,incorporated
R1308	T1943	T1944	det	a,strategy
R1309	T1944	T1942	dobj	strategy,using
R1310	T1945	T1946	nummod	four,primer
R1311	T1946	T1944	compound	primer,strategy
R1312	T1947	T1946	punct	-,primer
R1313	T1948	T1914	punct	: ,generated
R1314	T1949	T1914	advmod	first,generated
R1315	T1950	T1914	punct	", ",generated
R1316	T1951	T1952	nummod	two,products
R1317	T1952	T1914	nsubjpass	products,generated
R1318	T1953	T1954	advmod	partially,overlapping
R1319	T1954	T1952	amod	overlapping,products
R1320	T1955	T1914	auxpass	were,generated
R1321	T1956	T1914	prep	in,generated
R1322	T1957	T1958	amod	separate,reactions
R1323	T1958	T1956	pobj	reactions,in
R1324	T1959	T1914	advcl	using,generated
R1325	T1960	T1959	dobj	primers,using
R1326	T1961	T1962	dep	that,encode
R1327	T1962	T1960	relcl	encode,primers
R1328	T1963	T1964	det	the,mutations
R1329	T1964	T1962	dobj	mutations,encode
R1330	T1965	T1964	amod	desired,mutations
R1331	T1966	T1967	punct	(,M13
R1332	T1967	T1962	parataxis	M13,encode
R1333	T1968	T1969	compound	Swedish,forward
R1334	T1969	T1970	dep	forward,CCG
R1335	T1970	T1983	dep	CCG,C
R1336	T1971	T1970	punct	: ,CCG
R1337	T1972	T1970	compound	GGA,CCG
R1338	T1973	T1970	compound	GAT,CCG
R1339	T1974	T1970	compound	CTC,CCG
R1340	T1975	T1970	compound	TGA,CCG
R1341	T1976	T1970	compound	AGT,CCG
R1342	T1977	T1970	compound	GAA,CCG
R1343	T1978	T1970	compound	TCT,CCG
R1344	T1979	T1970	compound	GGA,CCG
R1345	T1980	T1970	compound	TGC,CCG
R1346	T1981	T1970	compound	AGA,CCG
R1347	T1982	T1970	compound	ATT,CCG
R1348	T1983	T1967	dep	C,M13
R1349	T1984	T1983	punct	/,C
R1350	T1985	T1986	compound	Indiana,reverse
R1351	T1986	T1983	dep	reverse,C
R1352	T1987	T1983	punct	: ,C
R1353	T1988	T1983	compound	GGG,C
R1354	T1989	T1983	compound	TGA,C
R1355	T1990	T1983	compound	TGA,C
R1356	T1991	T1983	compound	AAA,C
R1357	T1992	T1983	compound	TCA,C
R1358	T1993	T1983	compound	CGG,C
R1359	T1994	T1983	compound	TTG,C
R1360	T1995	T1967	punct	;,M13
R1361	T1996	T1997	compound	Indiana,forward
R1362	T1997	T1967	dep	forward,M13
R1363	T1998	T1967	punct	: ,M13
R1364	T1999	T1967	compound	CAA,M13
R1365	T2000	T1967	compound	CCG,M13
R1366	T2001	T1967	compound	TGA,M13
R1367	T2002	T1967	compound	TTT,M13
R1368	T2003	T1967	compound	TCA,M13
R1369	T2004	T1967	compound	TCA,M13
R1370	T2005	T1967	compound	CCC,M13
R1371	T2006	T1967	compound	TGG,M13
R1372	T2007	T1967	punct	/,M13
R1373	T2008	T1967	advmod	reverse,M13
R1374	T2009	T1967	punct	),M13
R1375	T2010	T1914	punct	.,generated
R1376	T2012	T2013	det	The,products
R1377	T2013	T2016	nsubjpass	products,ligated
R1378	T2014	T2013	nummod	two,products
R1379	T2015	T2013	compound	PCR,products
R1380	T2017	T2016	auxpass	were,ligated
R1381	T2018	T2016	punct	", ",ligated
R1382	T2019	T2016	dep	digested,ligated
R1383	T2020	T2016	prep	with,ligated
R1384	T2021	T2020	pobj	BglII,with
R1385	T2022	T2021	cc	and,BglII
R1386	T2023	T2021	conj	ApaI,BglII
R1387	T2024	T2016	cc	and,ligated
R1388	T2025	T2016	conj	cloned,ligated
R1389	T2026	T2025	advmod	back,cloned
R1390	T2027	T2025	prep	into,cloned
R1391	T2028	T2029	det	the,vector
R1392	T2029	T2027	pobj	vector,into
R1393	T2030	T2029	amod	original,vector
R1394	T2031	T2032	compound	mo,KS
R1395	T2032	T2029	compound	KS,vector
R1396	T2033	T2032	punct	/,KS
R1397	T2034	T2032	compound	huAPP695,KS
R1398	T2035	T2032	punct	-,KS
R1399	T2036	T2032	compound	BS,KS
R1400	T2037	T2032	punct	-,KS
R1401	T2038	T2016	punct	.,ligated
R1402	T2040	T2041	advmod	Finally,subcloned
R1403	T2042	T2041	punct	", ",subcloned
R1404	T2043	T2044	det	the,ind
R1405	T2044	T2041	nsubjpass	ind,subcloned
R1406	T2045	T2044	amod	new,ind
R1407	T2046	T2044	compound	APP695swe,ind
R1408	T2047	T2044	punct	/,ind
R1409	T2048	T2041	auxpass	was,subcloned
R1410	T2049	T2041	prep	into,subcloned
R1411	T2050	T2051	det	the,site
R1412	T2051	T2049	pobj	site,into
R1413	T2052	T2051	compound	XhoI,site
R1414	T2053	T2051	prep	of,site
R1415	T2054	T2055	det	the,vector
R1416	T2055	T2053	pobj	vector,of
R1417	T2056	T2057	compound	moPrP,tetP
R1418	T2057	T2055	compound	tetP,vector
R1419	T2058	T2057	punct	-,tetP
R1420	T2059	T2041	prep	from,subcloned
R1421	T2060	T2059	pcomp	above,from
R1422	T2061	T2062	aux	to,complete
R1423	T2062	T2041	advcl	complete,subcloned
R1424	T2063	T2064	det	the,construct
R1425	T2064	T2062	dobj	construct,complete
R1426	T2065	T2041	punct	.,subcloned
R1427	T2158	T2159	amod	Pronuclear,Injection
R1428	T2160	T2159	punct	", ",Injection
R1429	T2161	T2159	conj	Screening,Injection
R1430	T2162	T2161	prep	of,Screening
R1431	T2163	T2162	pobj	Founders,of
R1432	T2164	T2161	punct	", ",Screening
R1433	T2165	T2161	cc	and,Screening
R1434	T2166	T2161	conj	Maintenance,Screening
R1435	T2167	T2166	prep	of,Maintenance
R1436	T2168	T2169	det	the,Lines
R1437	T2169	T2167	pobj	Lines,of
R1438	T2171	T2172	det	The,vector
R1439	T2172	T2182	nsubjpass	vector,linearized
R1440	T2173	T2174	compound	moPrP,ind
R1441	T2174	T2172	compound	ind,vector
R1442	T2175	T2174	punct	-,ind
R1443	T2176	T2174	compound	tetP,ind
R1444	T2177	T2174	punct	-,ind
R1445	T2178	T2174	compound	mo,ind
R1446	T2179	T2174	punct	/,ind
R1447	T2180	T2174	compound	huAPP695swe,ind
R1448	T2181	T2174	punct	/,ind
R1449	T2183	T2182	auxpass	was,linearized
R1450	T2184	T2182	cc	and,linearized
R1451	T2185	T2186	det	the,domain
R1452	T2186	T2188	nsubj	domain,excised
R1453	T2187	T2186	compound	pBluescript,domain
R1454	T2188	T2182	conj	excised,linearized
R1455	T2189	T2188	prep	by,excised
R1456	T2190	T2189	pobj	digestion,by
R1457	T2191	T2190	prep	with,digestion
R1458	T2192	T2191	pobj	NotI,with
R1459	T2193	T2188	punct	.,excised
R1460	T2195	T2196	det	The,vector
R1461	T2196	T2198	nsubjpass	vector,injected
R1462	T2197	T2196	amod	purified,vector
R1463	T2199	T2198	auxpass	was,injected
R1464	T2200	T2198	prep	into,injected
R1465	T2201	T2202	det	the,pronucleus
R1466	T2202	T2200	pobj	pronucleus,into
R1467	T2203	T2202	prep	of,pronucleus
R1468	T2204	T2205	amod	fertilized,eggs
R1469	T2205	T2203	pobj	eggs,of
R1470	T2206	T2205	prep	from,eggs
R1471	T2207	T2208	nmod	C57BL,C3HeJ
R1472	T2208	T2212	compound	C3HeJ,matings
R1473	T2209	T2208	punct	/,C3HeJ
R1474	T2210	T2208	nmod	6J,C3HeJ
R1475	T2211	T2208	punct	×,C3HeJ
R1476	T2212	T2206	pobj	matings,from
R1477	T2213	T2212	compound	F1,matings
R1478	T2214	T2198	punct	.,injected
R1479	T2216	T2217	compound	Founder,animals
R1480	T2217	T2218	nsubjpass	animals,screened
R1481	T2219	T2218	auxpass	were,screened
R1482	T2220	T2218	prep	for,screened
R1483	T2221	T2222	det	the,presence
R1484	T2222	T2220	pobj	presence,for
R1485	T2223	T2222	prep	of,presence
R1486	T2224	T2225	det	the,transgene
R1487	T2225	T2223	pobj	transgene,of
R1488	T2226	T2218	prep	by,screened
R1489	T2227	T2228	nummod	three,way
R1490	T2228	T2230	compound	way,PCR
R1491	T2229	T2228	punct	-,way
R1492	T2230	T2226	pobj	PCR,by
R1493	T2231	T2218	advcl	using,screened
R1494	T2232	T2233	det	the,primers
R1495	T2233	T2231	dobj	primers,using
R1496	T2234	T2233	nmod	S36,primers
R1497	T2235	T2234	cc	and,S36
R1498	T2236	T2237	compound	PrP,AS
R1499	T2237	T2234	conj	AS,S36
R1500	T2238	T2237	punct	-,AS
R1501	T2239	T2237	compound	S,AS
R1502	T2240	T2237	punct	/,AS
R1503	T2241	T2237	compound	PrP,AS
R1504	T2242	T2237	punct	-,AS
R1505	T2243	T2233	acl	described,primers
R1506	T2244	T2243	advmod	below,described
R1507	T2245	T2218	punct	.,screened
R1508	T2247	T2248	npadvmod	Transgene,positive
R1509	T2248	T2250	amod	positive,founders
R1510	T2249	T2248	punct	-,positive
R1511	T2250	T2251	nsubjpass	founders,bred
R1512	T2252	T2251	auxpass	were,bred
R1513	T2253	T2251	prep	to,bred
R1514	T2254	T2253	pobj	animals,to
R1515	T2255	T2254	acl	expressing,animals
R1516	T2256	T2257	det	the,transactivator
R1517	T2257	T2255	dobj	transactivator,expressing
R1518	T2258	T2257	compound	tetracycline,transactivator
R1519	T2259	T2257	punct	(,transactivator
R1520	T2260	T2257	appos	tTA,transactivator
R1521	T2261	T2251	punct	),bred
R1522	T2262	T2251	prep	under,bred
R1523	T2263	T2262	pobj	control,under
R1524	T2264	T2263	prep	of,control
R1525	T2265	T2266	det	the,promoter
R1526	T2266	T2264	pobj	promoter,of
R1527	T2267	T2268	nmod	calcium,calmodulin
R1528	T2268	T2270	nmod	calmodulin,IIα
R1529	T2269	T2268	punct	-,calmodulin
R1530	T2270	T2266	nmod	IIα,promoter
R1531	T2271	T2270	nmod	kinase,IIα
R1532	T2272	T2266	punct	(,promoter
R1533	T2273	T2266	nmod	CaMKIIα,promoter
R1534	T2274	T2266	punct	),promoter
R1535	T2275	T2266	acl	obtained,promoter
R1536	T2276	T2275	prep	from,obtained
R1537	T2277	T2278	compound	Jackson,Laboratory
R1538	T2278	T2276	pobj	Laboratory,from
R1539	T2279	T2280	punct	[,16
R1540	T2280	T2278	parataxis	16,Laboratory
R1541	T2281	T2280	punct	],16
R1542	T2282	T2283	punct	(,Harbor
R1543	T2283	T2275	parataxis	Harbor,obtained
R1544	T2284	T2283	compound	Bar,Harbor
R1545	T2285	T2283	punct	", ",Harbor
R1546	T2286	T2283	npadvmod	Maine,Harbor
R1547	T2287	T2283	punct	", ",Harbor
R1548	T2288	T2289	compound	United,States
R1549	T2289	T2283	npadvmod	States,Harbor
R1550	T2290	T2283	punct	;,Harbor
R1551	T2291	T2292	dep	stock,1Mmay
R1552	T2292	T2283	dep	1Mmay,Harbor
R1553	T2293	T2291	punct	#,stock
R1554	T2294	T2291	nummod	3010,stock
R1555	T2295	T2292	punct	;,1Mmay
R1556	T2296	T2292	dep	B6,1Mmay
R1557	T2297	T2292	punct	;,1Mmay
R1558	T2298	T2292	nmod	CBA,1Mmay
R1559	T2299	T2292	punct	-,1Mmay
R1560	T2300	T2292	nmod	TgN,1Mmay
R1561	T2301	T2292	punct	[,1Mmay
R1562	T2302	T2292	nmod	Camk2a,1Mmay
R1563	T2303	T2292	punct	-,1Mmay
R1564	T2304	T2292	nmod	tTA,1Mmay
R1565	T2305	T2292	punct	],1Mmay
R1566	T2306	T2283	punct	),Harbor
R1567	T2307	T2251	punct	.,bred
R1568	T2309	T2310	det	The,colony
R1569	T2310	T2311	nsubjpass	colony,maintained
R1570	T2312	T2311	auxpass	was,maintained
R1571	T2313	T2311	advmod	thereafter,maintained
R1572	T2314	T2311	prep	by,maintained
R1573	T2315	T2314	pcomp	crossing,by
R1574	T2316	T2317	amod	single,offspring
R1575	T2317	T2315	dobj	offspring,crossing
R1576	T2318	T2317	amod	transgenic,offspring
R1577	T2319	T2317	nmod	tTA,offspring
R1578	T2320	T2319	cc	and,tTA
R1579	T2321	T2319	conj	APP,tTA
R1580	T2322	T2315	prep	for,crossing
R1581	T2323	T2322	pobj	each,for
R1582	T2324	T2323	prep	of,each
R1583	T2325	T2326	det	the,lines
R1584	T2326	T2324	pobj	lines,of
R1585	T2327	T2326	nummod	four,lines
R1586	T2328	T2326	compound	APP,lines
R1587	T2329	T2311	punct	.,maintained
R1588	T2331	T2332	det	All,mice
R1589	T2332	T2333	nsubjpass	mice,provided
R1590	T2334	T2333	auxpass	were,provided
R1591	T2335	T2336	amod	fresh,food
R1592	T2336	T2333	dobj	food,provided
R1593	T2337	T2336	cc	and,food
R1594	T2338	T2336	conj	water,food
R1595	T2339	T2340	advmod	ad,libitum
R1596	T2340	T2333	advmod	libitum,provided
R1597	T2341	T2333	punct	.,provided
R1598	T2343	T2344	compound	Animal,protocols
R1599	T2344	T2345	nsubjpass	protocols,approved
R1600	T2346	T2345	auxpass	were,approved
R1601	T2347	T2345	agent	by,approved
R1602	T2348	T2349	det	both,University
R1603	T2349	T2353	nmod	University,Committees
R1604	T2350	T2349	det	the,University
R1605	T2351	T2349	nmod	Johns,University
R1606	T2352	T2349	nmod	Hopkins,University
R1607	T2353	T2347	pobj	Committees,by
R1608	T2354	T2349	cc	and,University
R1609	T2355	T2356	det	the,Institute
R1610	T2356	T2349	conj	Institute,University
R1611	T2357	T2356	compound	California,Institute
R1612	T2358	T2356	prep	of,Institute
R1613	T2359	T2358	pobj	Technology,of
R1614	T2360	T2361	nmod	Institutional,Animal
R1615	T2361	T2353	nmod	Animal,Committees
R1616	T2362	T2361	appos	Care,Animal
R1617	T2363	T2362	cc	and,Care
R1618	T2364	T2362	conj	Use,Care
R1619	T2365	T2345	punct	.,approved
R1622	T2408	T2409	compound	Doxycycline,Administration
R1623	T2411	T2412	nsubjpass	Doxycycline,administered
R1624	T2413	T2411	punct	(,Doxycycline
R1625	T2414	T2411	appos	dox,Doxycycline
R1626	T2415	T2411	punct	),Doxycycline
R1627	T2416	T2412	auxpass	was,administered
R1628	T2417	T2412	prep	through,administered
R1629	T2418	T2419	advmod	commercially,available
R1630	T2419	T2420	amod	available,chow
R1631	T2420	T2417	pobj	chow,through
R1632	T2421	T2422	npadvmod	dox,containing
R1633	T2422	T2420	amod	containing,chow
R1634	T2423	T2422	punct	-,containing
R1635	T2424	T2425	punct	(,BioServ
R1636	T2425	T2412	parataxis	BioServ,administered
R1637	T2426	T2425	punct	", ",BioServ
R1638	T2427	T2425	npadvmod	Frenchtown,BioServ
R1639	T2428	T2425	punct	", ",BioServ
R1640	T2429	T2430	compound	New,Jersey
R1641	T2430	T2425	npadvmod	Jersey,BioServ
R1642	T2431	T2425	punct	", ",BioServ
R1643	T2432	T2433	compound	United,States
R1644	T2433	T2425	npadvmod	States,BioServ
R1645	T2434	T2425	punct	),BioServ
R1646	T2435	T2412	punct	.,administered
R1647	T2437	T2438	det	The,chow
R1648	T2438	T2439	nsubj	chow,contained
R1649	T2439	T2440	ccomp	contained,was
R1650	T2441	T2442	nummod	200,mg
R1651	T2442	T2439	dobj	mg,contained
R1652	T2443	T2444	punct	/,kg
R1653	T2444	T2442	prep	kg,mg
R1654	T2445	T2442	prep	of,mg
R1655	T2446	T2445	pobj	antibiotic,of
R1656	T2447	T2440	punct	;,was
R1657	T2448	T2440	prep	based,was
R1658	T2449	T2448	prep	on,based
R1659	T2450	T2451	amod	estimated,consumption
R1660	T2451	T2449	pobj	consumption,on
R1661	T2452	T2451	prep	of,consumption
R1662	T2453	T2454	nummod	5,g
R1663	T2454	T2452	pobj	g,of
R1664	T2455	T2454	prep	per,g
R1665	T2456	T2455	pobj	mouse,per
R1666	T2457	T2454	prep	per,g
R1667	T2458	T2457	pobj	day,per
R1668	T2459	T2440	punct	", ",was
R1669	T2460	T2461	det	the,dose
R1670	T2461	T2440	nsubj	dose,was
R1671	T2462	T2461	amod	expected,dose
R1672	T2463	T2461	prep	to,dose
R1673	T2464	T2465	det	each,animal
R1674	T2465	T2463	pobj	animal,to
R1675	T2466	T2467	nummod	1,mg
R1676	T2467	T2468	compound	mg,dox
R1677	T2468	T2440	attr	dox,was
R1678	T2469	T2468	prep	per,dox
R1679	T2470	T2469	pobj	day,per
R1680	T2471	T2440	punct	.,was
R1681	T2473	T2474	det	The,animal
R1682	T2474	T2479	nsubj	animal,received
R1683	T2475	T2474	amod	average,animal
R1684	T2476	T2477	nummod	25,g
R1685	T2477	T2474	compound	g,animal
R1686	T2478	T2477	punct	-,g
R1687	T2480	T2479	advmod	therefore,received
R1688	T2481	T2482	nummod	40,μg
R1689	T2482	T2479	dobj	μg,received
R1690	T2483	T2482	prep	of,μg
R1691	T2484	T2483	pobj	dox,of
R1692	T2485	T2482	prep	per,μg
R1693	T2486	T2487	compound	gram,weight
R1694	T2487	T2485	pobj	weight,per
R1695	T2488	T2487	compound	body,weight
R1696	T2489	T2482	prep	per,μg
R1697	T2490	T2489	pobj	day,per
R1698	T2491	T2479	punct	.,received
R1699	T2493	T2494	nsubjpass	Chow,changed
R1700	T2495	T2494	auxpass	was,changed
R1701	T2496	T2497	quantmod	1,2
R1702	T2497	T2499	nummod	2,times
R1703	T2498	T2497	punct	–,2
R1704	T2499	T2494	npadvmod	times,changed
R1705	T2500	T2499	prep	per,times
R1706	T2501	T2500	pobj	week,per
R1707	T2502	T2503	aux	to,prevent
R1708	T2503	T2494	advcl	prevent,changed
R1709	T2504	T2503	dobj	breakdown,prevent
R1710	T2505	T2504	prep	of,breakdown
R1711	T2506	T2507	det	the,antibiotic
R1712	T2507	T2505	pobj	antibiotic,of
R1713	T2508	T2494	punct	.,changed
R1718	T2637	T2638	nsubjpass	Offspring,genotyped
R1719	T2639	T2638	auxpass	were,genotyped
R1720	T2640	T2638	prep	for,genotyped
R1721	T2641	T2642	det	the,presence
R1722	T2642	T2640	pobj	presence,for
R1723	T2643	T2642	prep	of,presence
R1724	T2644	T2645	det	each,transgene
R1725	T2645	T2643	pobj	transgene,of
R1726	T2646	T2638	prep	by,genotyped
R1727	T2647	T2648	compound	PCR,amplification
R1728	T2648	T2646	pobj	amplification,by
R1729	T2649	T2648	prep	of,amplification
R1730	T2650	T2651	amod	genomic,DNA
R1731	T2651	T2649	pobj	DNA,of
R1732	T2652	T2651	acl	extracted,DNA
R1733	T2653	T2652	prep	from,extracted
R1734	T2654	T2655	det	a,biopsy
R1735	T2655	T2653	pobj	biopsy,from
R1736	T2656	T2657	nummod	5,mm
R1737	T2657	T2655	compound	mm,biopsy
R1738	T2658	T2657	punct	-,mm
R1739	T2659	T2655	compound	tail,biopsy
R1740	T2660	T2638	punct	.,genotyped
R1741	T2662	T2663	nsubjpass	Tails,heated
R1742	T2664	T2663	auxpass	were,heated
R1743	T2665	T2663	prep	to,heated
R1744	T2666	T2667	nummod	95,°C
R1745	T2667	T2665	pobj	°C,to
R1746	T2668	T2663	prep	for,heated
R1747	T2669	T2670	nummod	45,min
R1748	T2670	T2668	pobj	min,for
R1749	T2671	T2663	prep	in,heated
R1750	T2672	T2673	nummod	250,μl
R1751	T2673	T2671	pobj	μl,in
R1752	T2674	T2673	prep	of,μl
R1753	T2675	T2676	nummod	50,mM
R1754	T2676	T2677	compound	mM,NaOH
R1755	T2677	T2674	pobj	NaOH,of
R1756	T2678	T2663	punct	", ",heated
R1757	T2679	T2663	dep	vortexed,heated
R1758	T2680	T2663	punct	", ",heated
R1759	T2681	T2682	advmod	then,neutralized
R1760	T2682	T2663	dep	neutralized,heated
R1761	T2683	T2682	prep	with,neutralized
R1762	T2684	T2685	det	an,volume
R1763	T2685	T2683	pobj	volume,with
R1764	T2686	T2685	amod	equal,volume
R1765	T2687	T2685	prep	of,volume
R1766	T2688	T2689	nummod	0.5,M
R1767	T2689	T2690	compound	M,HCl
R1768	T2690	T2687	pobj	HCl,of
R1769	T2691	T2690	compound	Tris,HCl
R1770	T2692	T2690	punct	-,HCl
R1771	T2693	T2694	punct	(,pH
R1772	T2694	T2690	parataxis	pH,HCl
R1773	T2695	T2694	nummod	5.5,pH
R1774	T2696	T2694	punct	),pH
R1775	T2697	T2663	punct	.,heated
R1776	T2699	T2700	nsubjpass	Debris,sedimented
R1777	T2701	T2700	auxpass	was,sedimented
R1778	T2702	T2700	prep	by,sedimented
R1779	T2703	T2702	pobj	centrifugation,by
R1780	T2704	T2700	punct	", ",sedimented
R1781	T2705	T2700	cc	and,sedimented
R1782	T2706	T2707	nummod	3,μl
R1783	T2707	T2708	nsubjpass	μl,used
R1784	T2708	T2700	conj	used,sedimented
R1785	T2709	T2707	prep	of,μl
R1786	T2710	T2709	pobj	supernatant,of
R1787	T2711	T2708	auxpass	was,used
R1788	T2712	T2708	prep	for,used
R1789	T2713	T2712	pobj	amplification,for
R1790	T2714	T2708	punct	.,used
R1791	T2716	T2717	nsubjpass	Genotyping,performed
R1792	T2718	T2716	prep	for,Genotyping
R1793	T2719	T2720	nmod	APP,transgenes
R1794	T2720	T2718	pobj	transgenes,for
R1795	T2721	T2719	cc	and,APP
R1796	T2722	T2719	conj	tTA,APP
R1797	T2723	T2717	auxpass	was,performed
R1798	T2724	T2717	prep	in,performed
R1799	T2725	T2726	det	the,reaction
R1800	T2726	T2724	pobj	reaction,in
R1801	T2727	T2726	amod	same,reaction
R1802	T2728	T2726	compound	PCR,reaction
R1803	T2729	T2717	punct	", ",performed
R1804	T2730	T2717	advcl	using,performed
R1805	T2731	T2732	nummod	five,primers
R1806	T2732	T2730	dobj	primers,using
R1807	T2733	T2732	amod	separate,primers
R1808	T2734	T2717	punct	.,performed
R1809	T2736	T2737	nsubjpass	APP,amplified
R1810	T2738	T2737	auxpass	was,amplified
R1811	T2739	T2737	advcl	using,amplified
R1812	T2740	T2741	advmod	forward,S36
R1813	T2741	T2739	dobj	S36,using
R1814	T2742	T2741	compound	primer,S36
R1815	T2743	T2741	acl	located,S36
R1816	T2744	T2743	prep	in,located
R1817	T2745	T2746	det	the,end
R1818	T2746	T2744	pobj	end,in
R1819	T2747	T2746	nummod	3,end
R1820	T2748	T2747	punct	′,3
R1821	T2749	T2746	prep	of,end
R1822	T2750	T2751	det	the,cDNA
R1823	T2751	T2749	pobj	cDNA,of
R1824	T2752	T2751	compound	APP,cDNA
R1825	T2753	T2754	punct	(,ATG
R1826	T2754	T2751	parataxis	ATG,cDNA
R1827	T2755	T2754	compound	CCG,ATG
R1828	T2756	T2754	compound	AGA,ATG
R1829	T2757	T2754	compound	TCT,ATG
R1830	T2758	T2754	compound	CTG,ATG
R1831	T2759	T2754	compound	AAG,ATG
R1832	T2760	T2754	compound	TGA,ATG
R1833	T2761	T2754	compound	AGA,ATG
R1834	T2762	T2754	compound	TGG,ATG
R1835	T2763	T2754	punct	),ATG
R1836	T2764	T2741	cc	and,S36
R1837	T2765	T2766	amod	reverse,J
R1838	T2766	T2741	conj	J,S36
R1839	T2767	T2766	compound	primer,J
R1840	T2768	T2766	compound	PrP,J
R1841	T2769	T2766	punct	-,J
R1842	T2770	T2766	compound	AS,J
R1843	T2771	T2766	punct	-,J
R1844	T2772	T2766	acl	located,J
R1845	T2773	T2772	prep	in,located
R1846	T2774	T2775	det	the,UTR
R1847	T2775	T2773	pobj	UTR,in
R1848	T2776	T2775	nummod	3,UTR
R1849	T2777	T2776	punct	′,3
R1850	T2778	T2775	prep	of,UTR
R1851	T2779	T2780	det	the,vector
R1852	T2780	T2778	pobj	vector,of
R1853	T2781	T2782	punct	(,GC
R1854	T2782	T2775	parataxis	GC,UTR
R1855	T2783	T2782	compound	CCA,GC
R1856	T2784	T2782	compound	AGC,GC
R1857	T2785	T2782	compound	CTA,GC
R1858	T2786	T2782	compound	GAC,GC
R1859	T2787	T2782	compound	CAC,GC
R1860	T2788	T2782	compound	GAG,GC
R1861	T2789	T2782	compound	AAT,GC
R1862	T2790	T2782	punct	),GC
R1863	T2791	T2737	punct	.,amplified
R1864	T2793	T2794	det	The,transgene
R1865	T2794	T2796	nsubjpass	transgene,detected
R1866	T2795	T2794	compound	tTA,transgene
R1867	T2797	T2796	auxpass	was,detected
R1868	T2798	T2796	advcl	using,detected
R1869	T2799	T2800	det	a,set
R1870	T2800	T2798	dobj	set,using
R1871	T2801	T2800	compound	primer,set
R1872	T2802	T2803	dep	that,amplified
R1873	T2803	T2800	relcl	amplified,set
R1874	T2804	T2803	prep	across,amplified
R1875	T2805	T2806	poss	its,subdomains
R1876	T2806	T2804	pobj	subdomains,across
R1877	T2807	T2806	nummod	two,subdomains
R1878	T2808	T2803	prep	with,amplified
R1879	T2809	T2808	pobj	tet,with
R1880	T2810	T2809	advmod	forward,tet
R1881	T2811	T2809	acl	located,tet
R1882	T2812	T2811	prep	within,located
R1883	T2813	T2814	det	the,repressor
R1884	T2814	T2812	pobj	repressor,within
R1885	T2815	T2816	compound	Tn10,tetracycline
R1886	T2816	T2814	compound	tetracycline,repressor
R1887	T2817	T2818	punct	(,AG
R1888	T2818	T2814	parataxis	AG,repressor
R1889	T2819	T2818	compound	CGC,AG
R1890	T2820	T2818	compound	TGT,AG
R1891	T2821	T2818	compound	GGG,AG
R1892	T2822	T2818	compound	GCA,AG
R1893	T2823	T2818	compound	TTT,AG
R1894	T2824	T2818	compound	TAC,AG
R1895	T2825	T2818	compound	TTT,AG
R1896	T2826	T2818	punct	),AG
R1897	T2827	T2809	cc	and,tet
R1898	T2828	T2809	conj	tet,tet
R1899	T2829	T2828	amod	reverse,tet
R1900	T2830	T2828	prep	within,tet
R1901	T2831	T2832	det	the,VP16
R1902	T2832	T2830	pobj	VP16,within
R1903	T2833	T2832	compound	HSV1,VP16
R1904	T2834	T2835	punct	(,GTC
R1905	T2835	T2832	parataxis	GTC,VP16
R1906	T2836	T2835	compound	CAT,GTC
R1907	T2837	T2835	compound	GTC,GTC
R1908	T2838	T2835	compound	CAG,GTC
R1909	T2839	T2835	compound	ATC,GTC
R1910	T2840	T2835	compound	GAA,GTC
R1911	T2841	T2835	compound	ATC,GTC
R1912	T2842	T2835	punct	),GTC
R1913	T2843	T2796	punct	.,detected
R1914	T2845	T2846	det	All,reactions
R1915	T2846	T2847	nsubj	reactions,amplified
R1916	T2848	T2847	punct	", ",amplified
R1917	T2849	T2850	mark	whether,positive
R1918	T2850	T2847	advcl	positive,amplified
R1919	T2851	T2850	npadvmod	transgene,positive
R1920	T2852	T2850	punct	-,positive
R1921	T2853	T2850	cc	or,positive
R1922	T2854	T2850	conj	not,positive
R1923	T2855	T2847	punct	", ",amplified
R1924	T2856	T2857	det	a,segment
R1925	T2857	T2847	dobj	segment,amplified
R1926	T2858	T2857	prep	of,segment
R1927	T2859	T2860	det	the,gene
R1928	T2860	T2858	pobj	gene,of
R1929	T2861	T2860	amod	endogenous,gene
R1930	T2862	T2860	compound	prion,gene
R1931	T2863	T2860	compound	protein,gene
R1932	T2864	T2847	prep	as,amplified
R1933	T2865	T2866	det	a,control
R1934	T2866	T2864	pobj	control,as
R1935	T2867	T2866	prep	for,control
R1936	T2868	T2869	compound	DNA,quality
R1937	T2869	T2867	pobj	quality,for
R1938	T2870	T2847	advcl	using,amplified
R1939	T2871	T2872	det	a,primer
R1940	T2872	T2870	dobj	primer,using
R1941	T2873	T2872	amod	forward,primer
R1942	T2874	T2872	punct	", ",primer
R1943	T2875	T2876	compound	PrP,J
R1944	T2876	T2872	appos	J,primer
R1945	T2877	T2876	punct	-,J
R1946	T2878	T2876	compound	S,J
R1947	T2879	T2876	punct	-,J
R1948	T2880	T2872	punct	", ",primer
R1949	T2881	T2872	amod	specific,primer
R1950	T2882	T2881	prep	to,specific
R1951	T2883	T2884	det	the,frame
R1952	T2884	T2882	pobj	frame,to
R1953	T2885	T2884	nmod	mouse,frame
R1954	T2886	T2884	nmod	PrP,frame
R1955	T2887	T2888	amod	open,reading
R1956	T2888	T2884	compound	reading,frame
R1957	T2889	T2890	punct	(,GG
R1958	T2890	T2884	parataxis	GG,frame
R1959	T2891	T2890	compound	GGG,GG
R1960	T2892	T2890	compound	ACT,GG
R1961	T2893	T2890	compound	ATG,GG
R1962	T2894	T2890	compound	TGG,GG
R1963	T2895	T2890	compound	ACT,GG
R1964	T2896	T2890	compound	GAT,GG
R1965	T2897	T2890	compound	GTC,GG
R1966	T2898	T2890	punct	),GG
R1967	T2899	T2872	cc	and,primer
R1968	T2900	T2901	det	a,primer
R1969	T2901	T2872	conj	primer,primer
R1970	T2902	T2901	amod	reverse,primer
R1971	T2903	T2901	punct	", ",primer
R1972	T2904	T2905	compound	PrP,J
R1973	T2905	T2901	appos	J,primer
R1974	T2906	T2905	punct	-,J
R1975	T2907	T2905	compound	AS,J
R1976	T2908	T2905	punct	-,J
R1977	T2909	T2901	punct	", ",primer
R1978	T2910	T2901	acl	shared,primer
R1979	T2911	T2910	agent	by,shared
R1980	T2912	T2913	det	the,UTR
R1981	T2913	T2911	pobj	UTR,by
R1982	T2914	T2913	nummod	3,UTR
R1983	T2915	T2914	punct	′,3
R1984	T2916	T2913	prep	of,UTR
R1985	T2917	T2918	det	the,gene
R1986	T2918	T2916	pobj	gene,of
R1987	T2919	T2918	amod	endogenous,gene
R1988	T2920	T2918	compound	PrP,gene
R1989	T2921	T2918	cc	and,gene
R1990	T2922	T2923	det	the,vector
R1991	T2923	T2918	conj	vector,gene
R1992	T2924	T2923	compound	transgene,vector
R1993	T2925	T2847	punct	.,amplified
R1994	T2927	T2928	compound	Amplification,reactions
R1995	T2928	T2929	nsubjpass	reactions,run
R1996	T2930	T2929	auxpass	were,run
R1997	T2931	T2929	prep	for,run
R1998	T2932	T2933	nummod	37,cycles
R1999	T2933	T2931	pobj	cycles,for
R2000	T2934	T2929	prep	at,run
R2001	T2935	T2936	nummod	94,°C
R2002	T2936	T2934	pobj	°C,at
R2003	T2937	T2929	prep	for,run
R2004	T2938	T2939	nummod	30,s
R2005	T2939	T2937	pobj	s,for
R2006	T2940	T2929	punct	", ",run
R2007	T2941	T2942	nummod	64,°C
R2008	T2942	T2929	conj	°C,run
R2009	T2943	T2942	prep	for,°C
R2010	T2944	T2945	nummod	1,min
R2011	T2945	T2943	pobj	min,for
R2012	T2946	T2942	punct	", ",°C
R2013	T2947	T2942	cc	and,°C
R2014	T2948	T2949	nummod	72,°C
R2015	T2949	T2942	conj	°C,°C
R2016	T2950	T2949	prep	for,°C
R2017	T2951	T2952	nummod	1,min
R2018	T2952	T2950	pobj	min,for
R2019	T2953	T2929	punct	.,run
R2020	T2955	T2956	det	All,samples
R2021	T2956	T2957	nsubj	samples,gave
R2022	T2958	T2956	punct	", ",samples
R2023	T2959	T2956	amod	transgenic,samples
R2024	T2960	T2959	cc	and,transgenic
R2025	T2961	T2962	amod	wild,type
R2026	T2962	T2959	conj	type,transgenic
R2027	T2963	T2962	punct	-,type
R2028	T2964	T2957	punct	", ",gave
R2029	T2965	T2966	det	a,product
R2030	T2966	T2957	dobj	product,gave
R2031	T2967	T2968	nummod	750,bp
R2032	T2968	T2966	compound	bp,product
R2033	T2969	T2968	punct	-,bp
R2034	T2970	T2966	prep	from,product
R2035	T2971	T2972	det	the,gene
R2036	T2972	T2970	pobj	gene,from
R2037	T2973	T2972	amod	endogenous,gene
R2038	T2974	T2972	compound	PrP,gene
R2039	T2975	T2957	punct	.,gave
R2040	T2977	T2978	det	The,transgene
R2041	T2978	T2980	nsubj	transgene,yielded
R2042	T2979	T2978	compound	APP,transgene
R2043	T2980	T2981	ccomp	yielded,fell
R2044	T2982	T2983	det	an,band
R2045	T2983	T2980	dobj	band,yielded
R2046	T2984	T2983	amod	additional,band
R2047	T2985	T2983	prep	at,band
R2048	T2986	T2987	nummod	400,bp
R2049	T2987	T2985	pobj	bp,at
R2050	T2988	T2981	punct	;,fell
R2051	T2989	T2990	det	the,product
R2052	T2990	T2981	nsubj	product,fell
R2053	T2991	T2990	compound	tTA,product
R2054	T2992	T2993	advmod	in,between
R2055	T2993	T2981	advmod	between,fell
R2056	T2994	T2981	prep	at,fell
R2057	T2995	T2996	nummod	480,bp
R2058	T2996	T2994	pobj	bp,at
R2059	T2997	T2981	punct	.,fell
R2060	T3141	T3142	compound	Immunoblotting,Quantitation
R2061	T3143	T3142	punct	/,Quantitation
R2062	T3145	T3146	amod	Frozen,tissue
R2063	T3146	T3151	nsubjpass	tissue,homogenized
R2064	T3147	T3146	amod	cortical,tissue
R2065	T3148	T3147	cc	or,cortical
R2066	T3149	T3150	amod	whole,forebrain
R2067	T3150	T3147	conj	forebrain,cortical
R2068	T3152	T3151	auxpass	was,homogenized
R2069	T3153	T3151	prep	by,homogenized
R2070	T3154	T3153	pobj	sonication,by
R2071	T3155	T3151	prep	in,homogenized
R2072	T3156	T3157	nummod	five,volumes
R2073	T3157	T3155	pobj	volumes,in
R2074	T3158	T3157	prep	of,volumes
R2075	T3159	T3160	npadvmod	phosphate,buffered
R2076	T3160	T3162	amod	buffered,saline
R2077	T3161	T3160	punct	-,buffered
R2078	T3162	T3158	pobj	saline,of
R2079	T3163	T3162	punct	(,saline
R2080	T3164	T3162	appos	PBS,saline
R2081	T3165	T3151	punct	),homogenized
R2082	T3166	T3151	prep	with,homogenized
R2083	T3167	T3168	nummod	5,mM
R2084	T3168	T3169	nmod	mM,inhibitors
R2085	T3169	T3166	pobj	inhibitors,with
R2086	T3170	T3169	nmod	EDTA,inhibitors
R2087	T3171	T3170	cc	and,EDTA
R2088	T3172	T3170	conj	protease,EDTA
R2089	T3173	T3174	punct	(,cocktail
R2090	T3174	T3169	parataxis	cocktail,inhibitors
R2091	T3175	T3174	amod	Mammalian,cocktail
R2092	T3176	T3174	compound	cell,cocktail
R2093	T3177	T3174	punct	", ",cocktail
R2094	T3178	T3174	npadvmod	Sigma,cocktail
R2095	T3179	T3174	punct	", ",cocktail
R2096	T3180	T3181	compound	St.,Louis
R2097	T3181	T3174	npadvmod	Louis,cocktail
R2098	T3182	T3174	punct	", ",cocktail
R2099	T3183	T3174	npadvmod	Missouri,cocktail
R2100	T3184	T3174	punct	", ",cocktail
R2101	T3185	T3186	compound	United,States
R2102	T3186	T3174	npadvmod	States,cocktail
R2103	T3187	T3174	punct	),cocktail
R2104	T3188	T3151	punct	", ",homogenized
R2105	T3189	T3151	advcl	using,homogenized
R2106	T3190	T3191	det	a,sonicator
R2107	T3191	T3189	dobj	sonicator,using
R2108	T3192	T3191	compound	probe,sonicator
R2109	T3193	T3191	acl	set,sonicator
R2110	T3194	T3193	prep	to,set
R2111	T3195	T3196	nummod	50,%
R2112	T3196	T3197	compound	%,output
R2113	T3197	T3194	pobj	output,to
R2114	T3198	T3199	punct	(,TEKMAR
R2115	T3199	T3191	parataxis	TEKMAR,sonicator
R2116	T3200	T3199	punct	", ",TEKMAR
R2117	T3201	T3199	npadvmod	Cincinnati,TEKMAR
R2118	T3202	T3199	punct	", ",TEKMAR
R2119	T3203	T3199	npadvmod	Ohio,TEKMAR
R2120	T3204	T3199	punct	", ",TEKMAR
R2121	T3205	T3206	compound	United,States
R2122	T3206	T3199	npadvmod	States,TEKMAR
R2123	T3207	T3199	punct	),TEKMAR
R2124	T3208	T3151	punct	.,homogenized
R2125	T3210	T3211	prep	After,centrifuged
R2126	T3212	T3210	pobj	dilution,After
R2127	T3213	T3212	prep	with,dilution
R2128	T3214	T3215	det	an,volume
R2129	T3215	T3213	pobj	volume,with
R2130	T3216	T3215	amod	equal,volume
R2131	T3217	T3215	prep	of,volume
R2132	T3218	T3219	compound	PBS,protease
R2133	T3219	T3223	compound	protease,inhibitor
R2134	T3220	T3219	punct	/,protease
R2135	T3221	T3219	compound	EDTA,protease
R2136	T3222	T3219	punct	/,protease
R2137	T3223	T3217	pobj	inhibitor,of
R2138	T3224	T3211	punct	", ",centrifuged
R2139	T3225	T3226	det	the,samples
R2140	T3226	T3211	nsubjpass	samples,centrifuged
R2141	T3227	T3211	auxpass	were,centrifuged
R2142	T3228	T3211	advmod	briefly,centrifuged
R2143	T3229	T3211	cc	and,centrifuged
R2144	T3230	T3231	det	the,supernatant
R2145	T3231	T3232	nsubj	supernatant,used
R2146	T3232	T3211	conj	used,centrifuged
R2147	T3233	T3232	prep	for,used
R2148	T3234	T3233	pobj	analysis,for
R2149	T3235	T3211	punct	.,centrifuged
R2150	T3237	T3238	nummod	Fifty,micrograms
R2151	T3238	T3239	nmod	micrograms,6E10
R2152	T3239	T3241	nsubjpass	6E10,loaded
R2153	T3240	T3239	punct	(,6E10
R2154	T3242	T3239	cc	and,6E10
R2155	T3243	T3239	conj	CT15,6E10
R2156	T3244	T3243	punct	),CT15
R2157	T3245	T3239	cc	or,6E10
R2158	T3246	T3247	nummod	5,μg
R2159	T3247	T3248	nmod	μg,22C11
R2160	T3248	T3239	conj	22C11,6E10
R2161	T3249	T3248	punct	(,22C11
R2162	T3250	T3248	punct	),22C11
R2163	T3251	T3239	prep	of,6E10
R2164	T3252	T3253	compound	brain,homogenate
R2165	T3253	T3251	pobj	homogenate,of
R2166	T3254	T3241	auxpass	was,loaded
R2167	T3255	T3241	prep	per,loaded
R2168	T3256	T3255	pobj	lane,per
R2169	T3257	T3241	prep	onto,loaded
R2170	T3258	T3259	nummod	7.5,%
R2171	T3259	T3260	nmod	%,PAGE
R2172	T3260	T3277	compound	PAGE,gels
R2173	T3261	T3259	punct	", ",%
R2174	T3262	T3263	nummod	10,%
R2175	T3263	T3259	conj	%,%
R2176	T3264	T3265	punct	–,%
R2177	T3265	T3263	prep	%,%
R2178	T3266	T3265	nummod	20,%
R2179	T3267	T3263	punct	", ",%
R2180	T3268	T3263	cc	or,%
R2181	T3269	T3270	nummod	4,%
R2182	T3270	T3263	conj	%,%
R2183	T3271	T3272	punct	–,%
R2184	T3272	T3270	prep	%,%
R2185	T3273	T3272	nummod	20,%
R2186	T3274	T3260	compound	Tris,PAGE
R2187	T3275	T3260	punct	-,PAGE
R2188	T3276	T3260	compound	HCl,PAGE
R2189	T3277	T3257	pobj	gels,onto
R2190	T3278	T3279	punct	(,Laboratories
R2191	T3279	T3277	parataxis	Laboratories,gels
R2192	T3280	T3281	compound	Bio,Rad
R2193	T3281	T3279	compound	Rad,Laboratories
R2194	T3282	T3281	punct	-,Rad
R2195	T3283	T3279	punct	", ",Laboratories
R2196	T3284	T3279	npadvmod	Hercules,Laboratories
R2197	T3285	T3279	punct	", ",Laboratories
R2198	T3286	T3279	npadvmod	California,Laboratories
R2199	T3287	T3279	punct	", ",Laboratories
R2200	T3288	T3289	compound	United,States
R2201	T3289	T3279	npadvmod	States,Laboratories
R2202	T3290	T3279	punct	),Laboratories
R2203	T3291	T3241	cc	and,loaded
R2204	T3292	T3241	conj	electrophoresed,loaded
R2205	T3293	T3292	prep	for,electrophoresed
R2206	T3294	T3295	amod	several,hours
R2207	T3295	T3293	pobj	hours,for
R2208	T3296	T3292	prep	in,electrophoresed
R2209	T3297	T3298	nummod	1,sulfate
R2210	T3298	T3306	nmod	sulfate,buffer
R2211	T3299	T3297	punct	×,1
R2212	T3300	T3298	nmod	Tris,sulfate
R2213	T3301	T3298	punct	-,sulfate
R2214	T3302	T3298	nmod	glycine,sulfate
R2215	T3303	T3298	punct	–,sulfate
R2216	T3304	T3298	nmod	sodium,sulfate
R2217	T3305	T3298	nmod	dodecyl,sulfate
R2218	T3306	T3296	pobj	buffer,in
R2219	T3307	T3298	punct	(,sulfate
R2220	T3308	T3309	nummod	1,SDS
R2221	T3309	T3298	appos	SDS,sulfate
R2222	T3310	T3308	punct	×,1
R2223	T3311	T3309	compound	TG,SDS
R2224	T3312	T3309	punct	-,SDS
R2225	T3313	T3306	punct	),buffer
R2226	T3314	T3315	punct	(,Amresco
R2227	T3315	T3306	parataxis	Amresco,buffer
R2228	T3316	T3315	dep	6E10,Amresco
R2229	T3317	T3316	cc	and,6E10
R2230	T3318	T3316	conj	22C11,6E10
R2231	T3319	T3315	punct	;,Amresco
R2232	T3320	T3315	punct	", ",Amresco
R2233	T3321	T3315	npadvmod	Solon,Amresco
R2234	T3322	T3315	punct	", ",Amresco
R2235	T3323	T3315	npadvmod	Ohio,Amresco
R2236	T3324	T3315	punct	", ",Amresco
R2237	T3325	T3326	compound	United,States
R2238	T3326	T3315	npadvmod	States,Amresco
R2239	T3327	T3315	punct	),Amresco
R2240	T3328	T3306	cc	or,buffer
R2241	T3329	T3330	nummod	1,buffer
R2242	T3330	T3306	conj	buffer,buffer
R2243	T3331	T3329	punct	×,1
R2244	T3332	T3333	compound	Tris,SDS
R2245	T3333	T3330	compound	SDS,buffer
R2246	T3334	T3333	punct	-,SDS
R2247	T3335	T3333	compound	tricine,SDS
R2248	T3336	T3333	punct	-,SDS
R2249	T3337	T3338	punct	(,Invitrogen
R2250	T3338	T3330	parataxis	Invitrogen,buffer
R2251	T3339	T3338	dep	CT15,Invitrogen
R2252	T3340	T3338	punct	;,Invitrogen
R2253	T3341	T3338	punct	", ",Invitrogen
R2254	T3342	T3338	npadvmod	Carlsbad,Invitrogen
R2255	T3343	T3338	punct	", ",Invitrogen
R2256	T3344	T3338	npadvmod	California,Invitrogen
R2257	T3345	T3338	punct	", ",Invitrogen
R2258	T3346	T3347	compound	United,States
R2259	T3347	T3338	npadvmod	States,Invitrogen
R2260	T3348	T3338	punct	),Invitrogen
R2261	T3349	T3241	punct	.,loaded
R2262	T3351	T3352	nsubjpass	Proteins,transferred
R2263	T3353	T3352	auxpass	were,transferred
R2264	T3354	T3352	advmod	overnight,transferred
R2265	T3355	T3352	prep	to,transferred
R2266	T3356	T3357	nummod	0.45,μm
R2267	T3357	T3359	compound	μm,nitrocellulose
R2268	T3358	T3357	punct	-,μm
R2269	T3359	T3355	pobj	nitrocellulose,to
R2270	T3360	T3359	compound	Optitran,nitrocellulose
R2271	T3361	T3362	punct	(,Schleicher
R2272	T3362	T3359	parataxis	Schleicher,nitrocellulose
R2273	T3363	T3362	cc	and,Schleicher
R2274	T3364	T3362	conj	Schuell,Schleicher
R2275	T3365	T3362	punct	", ",Schleicher
R2276	T3366	T3362	npadvmod	Keene,Schleicher
R2277	T3367	T3362	punct	", ",Schleicher
R2278	T3368	T3369	compound	New,Hampshire
R2279	T3369	T3362	npadvmod	Hampshire,Schleicher
R2280	T3370	T3362	punct	", ",Schleicher
R2281	T3371	T3372	compound	United,States
R2282	T3372	T3362	npadvmod	States,Schleicher
R2283	T3373	T3362	punct	),Schleicher
R2284	T3374	T3352	prep	in,transferred
R2285	T3375	T3376	nummod	1,buffer
R2286	T3376	T3374	pobj	buffer,in
R2287	T3377	T3375	punct	×,1
R2288	T3378	T3376	compound	TG,buffer
R2289	T3379	T3380	punct	(,Amresco
R2290	T3380	T3376	parataxis	Amresco,buffer
R2291	T3381	T3380	punct	),Amresco
R2292	T3382	T3352	punct	.,transferred
R2293	T3384	T3385	nsubjpass	Blots,blocked
R2294	T3386	T3385	auxpass	were,blocked
R2295	T3387	T3385	prep	in,blocked
R2296	T3388	T3387	pobj	PBS,in
R2297	T3389	T3388	acl	containing,PBS
R2298	T3390	T3391	nummod	5,%
R2299	T3391	T3392	nmod	%,powder
R2300	T3392	T3389	dobj	powder,containing
R2301	T3393	T3394	amod	nonfat,milk
R2302	T3394	T3392	compound	milk,powder
R2303	T3395	T3394	amod	dry,milk
R2304	T3396	T3385	punct	", ",blocked
R2305	T3397	T3385	cc	and,blocked
R2306	T3398	T3385	conj	incubated,blocked
R2307	T3399	T3398	prep	for,incubated
R2308	T3400	T3401	nummod	3,h
R2309	T3401	T3399	pobj	h,for
R2310	T3402	T3398	prep	at,incubated
R2311	T3403	T3404	compound	room,temperature
R2312	T3404	T3402	pobj	temperature,at
R2313	T3405	T3398	prep	in,incubated
R2314	T3406	T3407	compound	blocking,solution
R2315	T3407	T3405	pobj	solution,in
R2316	T3408	T3407	prep	with,solution
R2317	T3409	T3408	pobj	one,with
R2318	T3410	T3409	prep	of,one
R2319	T3411	T3412	det	the,antibodies
R2320	T3412	T3410	pobj	antibodies,of
R2321	T3413	T3412	amod	following,antibodies
R2322	T3414	T3412	punct	: ,antibodies
R2323	T3415	T3416	nmod	mouse,22C11
R2324	T3416	T3412	appos	22C11,antibodies
R2325	T3417	T3416	amod	monoclonal,22C11
R2326	T3418	T3419	punct	(,17
R2327	T3419	T3416	parataxis	17,22C11
R2328	T3420	T3421	amod	kind,gift
R2329	T3421	T3419	dep	gift,17
R2330	T3422	T3421	prep	of,gift
R2331	T3423	T3424	compound	Konrad,Beyreuther
R2332	T3424	T3422	pobj	Beyreuther,of
R2333	T3425	T3424	cc	and,Beyreuther
R2334	T3426	T3427	compound	Andreas,Weidemann
R2335	T3427	T3424	conj	Weidemann,Beyreuther
R2336	T3428	T3419	punct	;,17
R2337	T3429	T3419	punct	[,17
R2338	T3430	T3419	punct	],17
R2339	T3431	T3419	punct	),17
R2340	T3432	T3416	acl	diluted,22C11
R2341	T3433	T3432	npadvmod	1,diluted
R2342	T3434	T3435	punct	:,"1,000"
R2343	T3435	T3433	prep	"1,000",1
R2344	T3436	T3416	punct	", ",22C11
R2345	T3437	T3438	nmod	mouse,6E10
R2346	T3438	T3416	conj	6E10,22C11
R2347	T3439	T3438	amod	monoclonal,6E10
R2348	T3440	T3441	punct	(,Laboratories
R2349	T3441	T3438	parataxis	Laboratories,6E10
R2350	T3442	T3441	compound	Signet,Laboratories
R2351	T3443	T3441	punct	", ",Laboratories
R2352	T3444	T3441	npadvmod	Dedham,Laboratories
R2353	T3445	T3441	punct	", ",Laboratories
R2354	T3446	T3441	npadvmod	Massachusetts,Laboratories
R2355	T3447	T3441	punct	", ",Laboratories
R2356	T3448	T3449	compound	United,States
R2357	T3449	T3441	npadvmod	States,Laboratories
R2358	T3450	T3441	punct	),Laboratories
R2359	T3451	T3438	acl	diluted,6E10
R2360	T3452	T3451	npadvmod	1,diluted
R2361	T3453	T3454	punct	:,"2,500"
R2362	T3454	T3452	prep	"2,500",1
R2363	T3455	T3438	punct	", ",6E10
R2364	T3456	T3457	nmod	rabbit,hSOD1
R2365	T3457	T3438	conj	hSOD1,6E10
R2366	T3458	T3457	amod	polyclonal,hSOD1
R2367	T3459	T3457	amod	anti-superoxide,hSOD1
R2368	T3460	T3457	nmod	dismutase,hSOD1
R2369	T3461	T3457	nummod	1,hSOD1
R2370	T3462	T3457	punct	(,hSOD1
R2371	T3463	T3457	compound	m,hSOD1
R2372	T3464	T3457	punct	/,hSOD1
R2373	T3465	T3457	punct	),hSOD1
R2374	T3466	T3467	punct	[,18
R2375	T3467	T3457	parataxis	18,hSOD1
R2376	T3468	T3467	punct	],18
R2377	T3469	T3457	acl	diluted,hSOD1
R2378	T3470	T3469	npadvmod	1,diluted
R2379	T3471	T3472	punct	:,"2,500"
R2380	T3472	T3470	prep	"2,500",1
R2381	T3473	T3470	prep	to,1
R2382	T3474	T3473	pobj	1,to
R2383	T3475	T3476	punct	:,"4,000"
R2384	T3476	T3474	prep	"4,000",1
R2385	T3477	T3457	punct	", ",hSOD1
R2386	T3478	T3457	cc	or,hSOD1
R2387	T3479	T3480	nmod	rabbit,CT15
R2388	T3480	T3457	conj	CT15,hSOD1
R2389	T3481	T3480	amod	polyclonal,CT15
R2390	T3482	T3483	punct	(,gift
R2391	T3483	T3480	parataxis	gift,CT15
R2392	T3484	T3483	amod	kind,gift
R2393	T3485	T3483	prep	of,gift
R2394	T3486	T3487	compound	Ed,Koo
R2395	T3487	T3485	pobj	Koo,of
R2396	T3488	T3483	punct	;,gift
R2397	T3489	T3490	punct	[,19
R2398	T3490	T3483	parataxis	19,gift
R2399	T3491	T3490	punct	],19
R2400	T3492	T3483	punct	),gift
R2401	T3493	T3480	acl	diluted,CT15
R2402	T3494	T3493	npadvmod	1,diluted
R2403	T3495	T3496	punct	:,"1,000"
R2404	T3496	T3494	prep	"1,000",1
R2405	T3497	T3385	punct	.,blocked
R2406	T3499	T3500	advmod	Subsequently,washed
R2407	T3501	T3500	punct	", ",washed
R2408	T3502	T3503	det	the,blots
R2409	T3503	T3500	nsubjpass	blots,washed
R2410	T3504	T3500	auxpass	were,washed
R2411	T3505	T3500	prep	with,washed
R2412	T3506	T3505	pobj	PBS,with
R2413	T3507	T3506	acl	containing,PBS
R2414	T3508	T3509	nummod	0.1,%
R2415	T3509	T3510	compound	%,Tween
R2416	T3510	T3507	dobj	Tween,containing
R2417	T3511	T3510	punct	-,Tween
R2418	T3512	T3510	nummod	20,Tween
R2419	T3513	T3500	punct	", ",washed
R2420	T3514	T3500	cc	and,washed
R2421	T3515	T3516	advmod	then,incubated
R2422	T3516	T3500	conj	incubated,washed
R2423	T3517	T3516	prep	with,incubated
R2424	T3518	T3519	preconj	either,goat
R2425	T3519	T3520	nmod	goat,antibody
R2426	T3520	T3517	pobj	antibody,with
R2427	T3521	T3519	amod	anti-mouse,goat
R2428	T3522	T3519	punct	–,goat
R2429	T3523	T3519	cc	or,goat
R2430	T3524	T3519	conj	goat,goat
R2431	T3525	T3524	amod	anti-rabbit,goat
R2432	T3526	T3520	punct	–,antibody
R2433	T3527	T3528	npadvmod	HRP,conjugated
R2434	T3528	T3520	amod	conjugated,antibody
R2435	T3529	T3520	amod	secondary,antibody
R2436	T3530	T3520	acl	diluted,antibody
R2437	T3531	T3530	npadvmod	1,diluted
R2438	T3532	T3533	punct	:,"1,000"
R2439	T3533	T3531	prep	"1,000",1
R2440	T3534	T3530	prep	in,diluted
R2441	T3535	T3536	compound	blocking,solution
R2442	T3536	T3534	pobj	solution,in
R2443	T3537	T3500	punct	.,washed
R2444	T3539	T3540	prep	After,developed
R2445	T3541	T3542	amod	several,rinses
R2446	T3542	T3539	pobj	rinses,After
R2447	T3543	T3542	amod	additional,rinses
R2448	T3544	T3542	prep	in,rinses
R2449	T3545	T3544	pobj	PBS,in
R2450	T3546	T3542	prep	with,rinses
R2451	T3547	T3548	nummod	0.1,Tween
R2452	T3548	T3546	pobj	Tween,with
R2453	T3549	T3547	quantmod	%,0.1
R2454	T3550	T3548	punct	-,Tween
R2455	T3551	T3548	nummod	20,Tween
R2456	T3552	T3540	punct	", ",developed
R2457	T3553	T3540	nsubjpass	blots,developed
R2458	T3554	T3540	auxpass	were,developed
R2459	T3555	T3540	prep	with,developed
R2460	T3556	T3557	amod	enhanced,reagent
R2461	T3557	T3555	pobj	reagent,with
R2462	T3558	T3557	compound	chemiluminescence,reagent
R2463	T3559	T3540	cc	and,developed
R2464	T3560	T3540	conj	imaged,developed
R2465	T3561	T3560	prep	with,imaged
R2466	T3562	T3563	det	the,system
R2467	T3563	T3561	pobj	system,with
R2468	T3564	T3565	compound	Bio,Rad
R2469	T3565	T3567	compound	Rad,FX
R2470	T3566	T3565	punct	-,Rad
R2471	T3567	T3563	compound	FX,system
R2472	T3568	T3567	compound	Molecular,FX
R2473	T3569	T3567	compound	Imager,FX
R2474	T3570	T3540	punct	.,developed
R2475	T3572	T3573	compound	Staining,intensity
R2476	T3573	T3574	nsubjpass	intensity,quantified
R2477	T3575	T3573	prep	within,intensity
R2478	T3576	T3577	det	each,lane
R2479	T3577	T3575	pobj	lane,within
R2480	T3578	T3574	auxpass	was,quantified
R2481	T3579	T3574	advcl	using,quantified
R2482	T3580	T3581	det	the,software
R2483	T3581	T3579	dobj	software,using
R2484	T3582	T3581	nmod	Quantity,software
R2485	T3583	T3582	nummod	One,Quantity
R2486	T3584	T3585	compound	image,analysis
R2487	T3585	T3581	compound	analysis,software
R2488	T3586	T3587	punct	(,FX
R2489	T3587	T3581	parataxis	FX,software
R2490	T3588	T3589	compound	Molecular,Imager
R2491	T3589	T3587	compound	Imager,FX
R2492	T3590	T3587	punct	", ",FX
R2493	T3591	T3592	compound	Bio,Rad
R2494	T3592	T3594	compound	Rad,Laboratories
R2495	T3593	T3592	punct	-,Rad
R2496	T3594	T3587	npadvmod	Laboratories,FX
R2497	T3595	T3587	punct	),FX
R2498	T3596	T3574	punct	.,quantified
R2499	T3598	T3599	nsubjpass	Background,calculated
R2500	T3600	T3599	auxpass	was,calculated
R2501	T3601	T3599	prep	from,calculated
R2502	T3602	T3601	prep	across,from
R2503	T3603	T3604	det	the,image
R2504	T3604	T3602	pobj	image,across
R2505	T3605	T3599	cc	and,calculated
R2506	T3606	T3599	conj	subtracted,calculated
R2507	T3607	T3606	prep	from,subtracted
R2508	T3608	T3609	det	the,file
R2509	T3609	T3607	pobj	file,from
R2510	T3610	T3609	amod	entire,file
R2511	T3611	T3599	punct	.,calculated
R2512	T3613	T3614	det	The,intensity
R2513	T3614	T3616	nsubjpass	intensity,calculated
R2514	T3615	T3614	compound	signal,intensity
R2515	T3617	T3614	prep	for,intensity
R2516	T3618	T3619	det	each,band
R2517	T3619	T3617	pobj	band,for
R2518	T3620	T3621	punct	(,intensity
R2519	T3621	T3614	parataxis	intensity,intensity
R2520	T3622	T3621	amod	corrected,intensity
R2521	T3623	T3621	compound	signal,intensity
R2522	T3624	T3621	punct	×,intensity
R2523	T3625	T3626	compound	pixel,number
R2524	T3626	T3621	appos	number,intensity
R2525	T3627	T3621	punct	),intensity
R2526	T3628	T3616	auxpass	was,calculated
R2527	T3629	T3616	advmod	then,calculated
R2528	T3630	T3616	advcl	using,calculated
R2529	T3631	T3632	det	the,tool
R2530	T3632	T3630	dobj	tool,using
R2531	T3633	T3634	compound	Volume,report
R2532	T3634	T3632	compound	report,tool
R2533	T3635	T3616	punct	.,calculated
R2534	T3710	T3711	compound	Slot,Blot
R2535	T3711	T3712	compound	Blot,Analysis
R2536	T3713	T3712	compound	mRNA,Analysis
R2537	T3715	T3716	nummod	Five,micrograms
R2538	T3716	T3717	nsubjpass	micrograms,filtered
R2539	T3718	T3716	prep	per,micrograms
R2540	T3719	T3718	pobj	sample,per
R2541	T3720	T3719	prep	of,sample
R2542	T3721	T3722	amod	total,RNA
R2543	T3722	T3720	pobj	RNA,of
R2544	T3723	T3722	acl	extracted,RNA
R2545	T3724	T3723	prep	from,extracted
R2546	T3725	T3726	amod	fresh,frozen
R2547	T3726	T3728	amod	frozen,brain
R2548	T3727	T3726	punct	-,frozen
R2549	T3728	T3724	pobj	brain,from
R2550	T3729	T3728	punct	", ",brain
R2551	T3730	T3728	conj	liver,brain
R2552	T3731	T3730	punct	", ",liver
R2553	T3732	T3730	conj	kidney,liver
R2554	T3733	T3732	punct	", ",kidney
R2555	T3734	T3732	conj	heart,kidney
R2556	T3735	T3734	punct	", ",heart
R2557	T3736	T3734	conj	lung,heart
R2558	T3737	T3736	punct	", ",lung
R2559	T3738	T3736	conj	spleen,lung
R2560	T3739	T3738	punct	", ",spleen
R2561	T3740	T3738	cc	and,spleen
R2562	T3741	T3742	amod	skeletal,muscle
R2563	T3742	T3738	conj	muscle,spleen
R2564	T3743	T3717	auxpass	was,filtered
R2565	T3744	T3717	dep	vacuum,filtered
R2566	T3745	T3717	punct	-,filtered
R2567	T3746	T3717	prep	through,filtered
R2568	T3747	T3748	nummod	0.45,μm
R2569	T3748	T3750	compound	μm,nitrocellulose
R2570	T3749	T3748	punct	-,μm
R2571	T3750	T3746	pobj	nitrocellulose,through
R2572	T3751	T3750	compound	Optitran,nitrocellulose
R2573	T3752	T3717	punct	.,filtered
R2574	T3754	T3755	prep	After,cross-linked
R2575	T3756	T3757	amod	several,washes
R2576	T3757	T3754	pobj	washes,After
R2577	T3758	T3757	prep	through,washes
R2578	T3759	T3760	det	the,manifold
R2579	T3760	T3758	pobj	manifold,through
R2580	T3761	T3757	prep	with,washes
R2581	T3762	T3763	nummod	10,SSC
R2582	T3763	T3761	pobj	SSC,with
R2583	T3764	T3762	punct	×,10
R2584	T3765	T3755	punct	", ",cross-linked
R2585	T3766	T3755	nsubjpass	blots,cross-linked
R2586	T3767	T3755	auxpass	were,cross-linked
R2587	T3768	T3755	dep	UV,cross-linked
R2588	T3769	T3755	punct	-,cross-linked
R2589	T3770	T3755	cc	and,cross-linked
R2590	T3771	T3755	conj	probed,cross-linked
R2591	T3772	T3771	prep	with,probed
R2592	T3773	T3774	det	a,fragment
R2593	T3774	T3772	pobj	fragment,with
R2594	T3775	T3774	amod	radiolabeled,fragment
R2595	T3776	T3777	punct	∼,350
R2596	T3777	T3778	nummod	350,bp
R2597	T3778	T3774	compound	bp,fragment
R2598	T3779	T3778	punct	-,bp
R2599	T3780	T3781	compound	BglII,XhoI
R2600	T3781	T3774	compound	XhoI,fragment
R2601	T3782	T3781	punct	–,XhoI
R2602	T3783	T3774	compound	cDNA,fragment
R2603	T3784	T3774	prep	of,fragment
R2604	T3785	T3786	compound	mo,huAPP695
R2605	T3786	T3788	compound	huAPP695,cDNA
R2606	T3787	T3786	punct	/,huAPP695
R2607	T3788	T3784	pobj	cDNA,of
R2608	T3789	T3755	punct	.,cross-linked
R2609	T3791	T3792	prep	After,washed
R2610	T3793	T3791	pcomp	hybridizing,After
R2611	T3794	T3793	advmod	overnight,hybridizing
R2612	T3795	T3793	prep	at,hybridizing
R2613	T3796	T3797	nummod	65,°C
R2614	T3797	T3795	pobj	°C,at
R2615	T3798	T3793	prep	in,hybridizing
R2616	T3799	T3800	nummod	1,%
R2617	T3800	T3801	compound	%,BSA
R2618	T3801	T3798	pobj	BSA,in
R2619	T3802	T3801	punct	/,BSA
R2620	T3803	T3804	nummod	1,mM
R2621	T3804	T3805	compound	mM,EDTA
R2622	T3805	T3801	appos	EDTA,BSA
R2623	T3806	T3801	punct	/,BSA
R2624	T3807	T3808	nummod	0.5,M
R2625	T3808	T3809	compound	M,buffer
R2626	T3809	T3801	appos	buffer,BSA
R2627	T3810	T3809	compound	sodium,buffer
R2628	T3811	T3809	compound	phosphate,buffer
R2629	T3812	T3813	punct	(,pH
R2630	T3813	T3809	parataxis	pH,buffer
R2631	T3814	T3813	nummod	7.2,pH
R2632	T3815	T3813	punct	),pH
R2633	T3816	T3801	punct	/,BSA
R2634	T3817	T3818	nummod	7,%
R2635	T3818	T3819	compound	%,SDS
R2636	T3819	T3801	appos	SDS,BSA
R2637	T3820	T3821	punct	[,20
R2638	T3821	T3819	parataxis	20,SDS
R2639	T3822	T3821	punct	],20
R2640	T3823	T3792	punct	", ",washed
R2641	T3824	T3825	det	the,blots
R2642	T3825	T3792	nsubjpass	blots,washed
R2643	T3826	T3792	auxpass	were,washed
R2644	T3827	T3792	advmod	twice,washed
R2645	T3828	T3792	prep	at,washed
R2646	T3829	T3830	nummod	65,°C
R2647	T3830	T3828	pobj	°C,at
R2648	T3831	T3792	prep	for,washed
R2649	T3832	T3833	nummod	30,min
R2650	T3833	T3831	pobj	min,for
R2651	T3834	T3833	appos	each,min
R2652	T3835	T3792	prep	in,washed
R2653	T3836	T3837	nummod	0.1,%
R2654	T3837	T3838	compound	%,BSA
R2655	T3838	T3835	pobj	BSA,in
R2656	T3839	T3838	punct	/,BSA
R2657	T3840	T3841	nummod	1,mM
R2658	T3841	T3842	compound	mM,EDTA
R2659	T3842	T3838	appos	EDTA,BSA
R2660	T3843	T3838	punct	/,BSA
R2661	T3844	T3845	nummod	40,mM
R2662	T3845	T3846	compound	mM,buffer
R2663	T3846	T3838	appos	buffer,BSA
R2664	T3847	T3846	compound	sodium,buffer
R2665	T3848	T3846	compound	phosphate,buffer
R2666	T3849	T3850	punct	(,pH
R2667	T3850	T3846	parataxis	pH,buffer
R2668	T3851	T3850	nummod	7.2,pH
R2669	T3852	T3850	punct	),pH
R2670	T3853	T3838	punct	/,BSA
R2671	T3854	T3855	nummod	5,%
R2672	T3855	T3856	compound	%,SDS
R2673	T3856	T3838	appos	SDS,BSA
R2674	T3857	T3792	prep	before,washed
R2675	T3858	T3859	nummod	two,washes
R2676	T3859	T3857	pobj	washes,before
R2677	T3860	T3859	amod	final,washes
R2678	T3861	T3862	nummod	30,min
R2679	T3862	T3859	compound	min,washes
R2680	T3863	T3862	punct	-,min
R2681	T3864	T3859	prep	at,washes
R2682	T3865	T3866	nummod	65,°C
R2683	T3866	T3864	pobj	°C,at
R2684	T3867	T3859	prep	with,washes
R2685	T3868	T3869	nummod	1,mM
R2686	T3869	T3870	compound	mM,EDTA
R2687	T3870	T3867	pobj	EDTA,with
R2688	T3871	T3870	punct	/,EDTA
R2689	T3872	T3873	nummod	40,mM
R2690	T3873	T3874	compound	mM,buffer
R2691	T3874	T3870	appos	buffer,EDTA
R2692	T3875	T3874	compound	sodium,buffer
R2693	T3876	T3874	compound	phosphate,buffer
R2694	T3877	T3878	punct	(,pH
R2695	T3878	T3874	parataxis	pH,buffer
R2696	T3879	T3878	nummod	7.2,pH
R2697	T3880	T3878	punct	),pH
R2698	T3881	T3870	punct	/,EDTA
R2699	T3882	T3883	nummod	1,%
R2700	T3883	T3884	compound	%,SDS
R2701	T3884	T3870	appos	SDS,EDTA
R2702	T3885	T3792	punct	.,washed
R2703	T3887	T3888	nsubjpass	Blots,wrapped
R2704	T3889	T3888	auxpass	were,wrapped
R2705	T3890	T3888	advcl	wet,wrapped
R2706	T3891	T3888	cc	and,wrapped
R2707	T3892	T3888	conj	exposed,wrapped
R2708	T3893	T3892	prep	to,exposed
R2709	T3894	T3893	pobj	phosphorscreens,to
R2710	T3895	T3892	advmod	overnight,exposed
R2711	T3896	T3892	prep	at,exposed
R2712	T3897	T3898	compound	room,temperature
R2713	T3898	T3896	pobj	temperature,at
R2714	T3899	T3888	punct	.,wrapped
R2715	T4137	T4138	compound	Amyloid,Histology
R2716	T4140	T4141	nsubjpass	Mice,euthanized
R2717	T4142	T4141	auxpass	were,euthanized
R2718	T4143	T4141	prep	by,euthanized
R2719	T4144	T4145	compound	ether,inhalation
R2720	T4145	T4143	pobj	inhalation,by
R2721	T4146	T4141	cc	and,euthanized
R2722	T4147	T4148	nsubj	brains,removed
R2723	T4148	T4141	conj	removed,euthanized
R2724	T4149	T4148	prep	for,removed
R2725	T4150	T4151	compound	immersion,fixation
R2726	T4151	T4149	pobj	fixation,for
R2727	T4152	T4151	prep	in,fixation
R2728	T4153	T4154	nummod	4,%
R2729	T4154	T4155	compound	%,paraformaldehyde
R2730	T4155	T4152	pobj	paraformaldehyde,in
R2731	T4156	T4155	punct	/,paraformaldehyde
R2732	T4157	T4158	nummod	1,PBS
R2733	T4158	T4155	appos	PBS,paraformaldehyde
R2734	T4159	T4157	punct	×,1
R2735	T4160	T4148	punct	.,removed
R2736	T4162	T4163	prep	After,transferred
R2737	T4164	T4165	nummod	48,h
R2738	T4165	T4162	pobj	h,After
R2739	T4166	T4165	prep	in,h
R2740	T4167	T4166	pobj	fixative,in
R2741	T4168	T4165	prep	at,h
R2742	T4169	T4170	nummod	4,°C
R2743	T4170	T4168	pobj	°C,at
R2744	T4171	T4163	punct	", ",transferred
R2745	T4172	T4163	nsubjpass	brains,transferred
R2746	T4173	T4163	auxpass	were,transferred
R2747	T4174	T4163	prep	to,transferred
R2748	T4175	T4174	pobj	PBS,to
R2749	T4176	T4163	punct	", ",transferred
R2750	T4177	T4163	conj	dehydrated,transferred
R2751	T4178	T4177	prep	in,dehydrated
R2752	T4179	T4178	pobj	alcohols,in
R2753	T4180	T4177	punct	", ",dehydrated
R2754	T4181	T4177	conj	treated,dehydrated
R2755	T4182	T4181	prep	with,treated
R2756	T4183	T4184	compound	cedarwood,oil
R2757	T4184	T4182	pobj	oil,with
R2758	T4185	T4184	cc	and,oil
R2759	T4186	T4184	conj	methylsalicylate,oil
R2760	T4187	T4181	punct	", ",treated
R2761	T4188	T4181	cc	and,treated
R2762	T4189	T4181	conj	embedded,treated
R2763	T4190	T4189	prep	in,embedded
R2764	T4191	T4190	pobj	paraffin,in
R2765	T4192	T4189	prep	for,embedded
R2766	T4193	T4192	pobj	sectioning,for
R2767	T4194	T4163	punct	.,transferred
R2768	T4196	T4197	compound	Hirano,stain
R2769	T4198	T4197	compound	silver,stain
R2770	T4200	T4201	compound	Silver,histology
R2771	T4201	T4203	nsubjpass	histology,performed
R2772	T4202	T4201	compound	impregnation,histology
R2773	T4204	T4203	auxpass	was,performed
R2774	T4205	T4203	prep	on,performed
R2775	T4206	T4207	nummod	10,μm
R2776	T4207	T4209	nmod	μm,sections
R2777	T4208	T4207	punct	-,μm
R2778	T4209	T4205	pobj	sections,on
R2779	T4210	T4211	npadvmod	paraffin,embedded
R2780	T4211	T4209	amod	embedded,sections
R2781	T4212	T4211	punct	-,embedded
R2782	T4213	T4203	prep	by,performed
R2783	T4214	T4215	poss	Hirano,modification
R2784	T4215	T4213	pobj	modification,by
R2785	T4216	T4214	case	's,Hirano
R2786	T4217	T4215	prep	of,modification
R2787	T4218	T4219	det	the,method
R2788	T4219	T4217	pobj	method,of
R2789	T4220	T4219	compound	Bielschowsky,method
R2790	T4221	T4222	punct	[,21
R2791	T4222	T4203	parataxis	21,performed
R2792	T4223	T4222	punct	],21
R2793	T4224	T4203	punct	.,performed
R2794	T4226	T4227	advmod	Briefly,deparaffinized
R2795	T4228	T4227	punct	", ",deparaffinized
R2796	T4229	T4227	nsubjpass	sections,deparaffinized
R2797	T4230	T4227	auxpass	were,deparaffinized
R2798	T4231	T4227	prep	through,deparaffinized
R2799	T4232	T4231	pobj	xylene,through
R2800	T4233	T4232	cc	and,xylene
R2801	T4234	T4232	conj	alcohols,xylene
R2802	T4235	T4227	prep	into,deparaffinized
R2803	T4236	T4237	compound	tap,water
R2804	T4237	T4235	pobj	water,into
R2805	T4238	T4227	prep	before,deparaffinized
R2806	T4239	T4240	auxpass	being,placed
R2807	T4240	T4238	pcomp	placed,before
R2808	T4241	T4240	prep	into,placed
R2809	T4242	T4243	amod	fresh,solution
R2810	T4243	T4241	pobj	solution,into
R2811	T4244	T4245	nummod	20,%
R2812	T4245	T4243	compound	%,solution
R2813	T4246	T4243	compound	silver,solution
R2814	T4247	T4243	compound	nitrate,solution
R2815	T4248	T4240	prep	for,placed
R2816	T4249	T4250	nummod	20,min
R2817	T4250	T4248	pobj	min,for
R2818	T4251	T4227	punct	.,deparaffinized
R2819	T4253	T4254	prep	After,immersed
R2820	T4255	T4256	auxpass	being,washed
R2821	T4256	T4253	pcomp	washed,After
R2822	T4257	T4256	advmod	thoroughly,washed
R2823	T4258	T4256	prep	with,washed
R2824	T4259	T4260	amod	distilled,water
R2825	T4260	T4258	pobj	water,with
R2826	T4261	T4254	punct	", ",immersed
R2827	T4262	T4254	nsubjpass	slides,immersed
R2828	T4263	T4254	auxpass	were,immersed
R2829	T4264	T4254	prep	in,immersed
R2830	T4265	T4266	nummod	20,%
R2831	T4266	T4267	compound	%,solution
R2832	T4267	T4264	pobj	solution,in
R2833	T4268	T4269	compound	silver,nitrate
R2834	T4269	T4267	compound	nitrate,solution
R2835	T4270	T4267	acl	titrated,solution
R2836	T4271	T4270	prep	with,titrated
R2837	T4272	T4273	amod	fresh,hydroxide
R2838	T4273	T4271	pobj	hydroxide,with
R2839	T4274	T4273	compound	ammonium,hydroxide
R2840	T4275	T4254	punct	.,immersed
R2841	T4277	T4278	prep	After,washed
R2842	T4279	T4280	nummod	20,min
R2843	T4280	T4277	pobj	min,After
R2844	T4281	T4278	punct	", ",washed
R2845	T4282	T4278	nsubjpass	slides,washed
R2846	T4283	T4278	auxpass	were,washed
R2847	T4284	T4278	prep	with,washed
R2848	T4285	T4286	compound	ammonia,water
R2849	T4286	T4284	pobj	water,with
R2850	T4287	T4278	prep	before,washed
R2851	T4288	T4289	auxpass	being,developed
R2852	T4289	T4287	pcomp	developed,before
R2853	T4290	T4289	advmod	individually,developed
R2854	T4291	T4289	prep	with,developed
R2855	T4292	T4293	nummod	100,μl
R2856	T4293	T4291	pobj	μl,with
R2857	T4294	T4293	prep	of,μl
R2858	T4295	T4294	pobj	developer,of
R2859	T4296	T4297	punct	(,ml
R2860	T4297	T4295	parataxis	ml,developer
R2861	T4298	T4297	nummod	20,ml
R2862	T4299	T4297	prep	of,ml
R2863	T4300	T4301	nummod	37,%
R2864	T4301	T4302	compound	%,formaldehyde
R2865	T4302	T4299	pobj	formaldehyde,of
R2866	T4303	T4297	punct	", ",ml
R2867	T4304	T4305	nummod	100,ml
R2868	T4305	T4297	conj	ml,ml
R2869	T4306	T4305	prep	of,ml
R2870	T4307	T4308	amod	distilled,water
R2871	T4308	T4306	pobj	water,of
R2872	T4309	T4305	punct	", ",ml
R2873	T4310	T4311	nummod	50,μl
R2874	T4311	T4305	conj	μl,ml
R2875	T4312	T4311	prep	of,μl
R2876	T4313	T4314	amod	concentrated,acid
R2877	T4314	T4312	pobj	acid,of
R2878	T4315	T4314	amod	nitric,acid
R2879	T4316	T4311	punct	", ",μl
R2880	T4317	T4311	cc	and,μl
R2881	T4318	T4319	nummod	0.5,g
R2882	T4319	T4311	conj	g,μl
R2883	T4320	T4319	prep	of,g
R2884	T4321	T4322	amod	citric,acid
R2885	T4322	T4320	pobj	acid,of
R2886	T4323	T4297	punct	),ml
R2887	T4324	T4293	acl	added,μl
R2888	T4325	T4324	prep	to,added
R2889	T4326	T4327	nummod	50,ml
R2890	T4327	T4325	pobj	ml,to
R2891	T4328	T4327	prep	of,ml
R2892	T4329	T4330	amod	titrated,solution
R2893	T4330	T4328	pobj	solution,of
R2894	T4331	T4332	compound	silver,nitrate
R2895	T4332	T4330	compound	nitrate,solution
R2896	T4333	T4278	punct	.,washed
R2897	T4335	T4336	nsubjpass	Slides,rinsed
R2898	T4337	T4336	auxpass	were,rinsed
R2899	T4338	T4336	advmod	then,rinsed
R2900	T4339	T4336	prep	in,rinsed
R2901	T4340	T4341	compound	tap,water
R2902	T4341	T4339	pobj	water,in
R2903	T4342	T4336	punct	", ",rinsed
R2904	T4343	T4336	conj	fixed,rinsed
R2905	T4344	T4343	prep	in,fixed
R2906	T4345	T4346	nummod	5,%
R2907	T4346	T4347	compound	%,thiosulfate
R2908	T4347	T4344	pobj	thiosulfate,in
R2909	T4348	T4347	compound	sodium,thiosulfate
R2910	T4349	T4343	punct	", ",fixed
R2911	T4350	T4343	cc	and,fixed
R2912	T4351	T4343	conj	dehydrated,fixed
R2913	T4352	T4351	prep	through,dehydrated
R2914	T4353	T4352	pobj	alcohols,through
R2915	T4354	T4353	cc	and,alcohols
R2916	T4355	T4353	conj	xylene,alcohols
R2917	T4356	T4336	punct	.,rinsed
R2918	T4358	T4359	compound	Thioflavin,S
R2919	T4359	T4361	compound	S,staining
R2920	T4360	T4359	punct	-,S
R2921	T4363	T4364	prep	Following,performed
R2922	T4365	T4363	pobj	deparaffinization,Following
R2923	T4366	T4365	prep	of,deparaffinization
R2924	T4367	T4366	pobj	sections,of
R2925	T4368	T4365	prep	through,deparaffinization
R2926	T4369	T4368	pobj	xylene,through
R2927	T4370	T4369	cc	and,xylene
R2928	T4371	T4369	conj	alcohols,xylene
R2929	T4372	T4364	punct	", ",performed
R2930	T4373	T4374	compound	amyloid,impregnation
R2931	T4374	T4364	nsubjpass	impregnation,performed
R2932	T4375	T4374	prep	with,impregnation
R2933	T4376	T4377	compound	thioflavin,S
R2934	T4377	T4375	pobj	S,with
R2935	T4378	T4377	punct	-,S
R2936	T4379	T4364	auxpass	was,performed
R2937	T4380	T4364	prep	according,performed
R2938	T4381	T4380	prep	to,according
R2939	T4382	T4383	det	the,modification
R2940	T4383	T4381	pobj	modification,to
R2941	T4384	T4383	compound	Guntern,modification
R2942	T4385	T4383	prep	of,modification
R2943	T4386	T4387	det	the,protocol
R2944	T4387	T4385	pobj	protocol,of
R2945	T4388	T4387	amod	standard,protocol
R2946	T4389	T4364	punct	.,performed
R2947	T4391	T4392	nsubjpass	Slides,washed
R2948	T4393	T4391	acl	holding,Slides
R2949	T4394	T4395	nummod	10,μm
R2950	T4395	T4397	compound	μm,sections
R2951	T4396	T4395	punct	-,μm
R2952	T4397	T4393	dobj	sections,holding
R2953	T4398	T4397	compound	paraffin,sections
R2954	T4399	T4392	auxpass	were,washed
R2955	T4400	T4392	advmod	twice,washed
R2956	T4401	T4392	prep	in,washed
R2957	T4402	T4403	amod	distilled,water
R2958	T4403	T4401	pobj	water,in
R2959	T4404	T4392	punct	", ",washed
R2960	T4405	T4406	advmod	then,immersed
R2961	T4406	T4392	dep	immersed,washed
R2962	T4407	T4406	prep	for,immersed
R2963	T4408	T4409	nummod	5,min
R2964	T4409	T4407	pobj	min,for
R2965	T4410	T4406	prep	in,immersed
R2966	T4411	T4412	det	a,solution
R2967	T4412	T4410	pobj	solution,in
R2968	T4413	T4414	nummod	0.25,%
R2969	T4414	T4412	compound	%,solution
R2970	T4415	T4412	compound	potassium,solution
R2971	T4416	T4412	compound	permanganate,solution
R2972	T4417	T4406	punct	", ",immersed
R2973	T4418	T4406	advcl	followed,immersed
R2974	T4419	T4418	agent	by,followed
R2975	T4420	T4421	nummod	5,min
R2976	T4421	T4419	pobj	min,by
R2977	T4422	T4421	prep	in,min
R2978	T4423	T4424	det	a,solution
R2979	T4424	T4422	pobj	solution,in
R2980	T4425	T4426	nummod	1,%
R2981	T4426	T4427	nmod	%,metabisulfate
R2982	T4427	T4424	nmod	metabisulfate,solution
R2983	T4428	T4427	nmod	potassium,metabisulfate
R2984	T4429	T4427	punct	/,metabisulfate
R2985	T4430	T4431	nummod	1,%
R2986	T4431	T4432	nmod	%,acid
R2987	T4432	T4427	appos	acid,metabisulfate
R2988	T4433	T4432	amod	oxalic,acid
R2989	T4434	T4392	punct	.,washed
R2990	T4436	T4437	prep	After,placed
R2991	T4438	T4439	det	this,preparation
R2992	T4439	T4436	pobj	preparation,After
R2993	T4440	T4437	punct	", ",placed
R2994	T4441	T4437	nsubjpass	slides,placed
R2995	T4442	T4437	auxpass	were,placed
R2996	T4443	T4437	prep	into,placed
R2997	T4444	T4445	det	a,solution
R2998	T4445	T4443	pobj	solution,into
R2999	T4446	T4445	amod	filtered,solution
R3000	T4447	T4445	amod	aqueous,solution
R3001	T4448	T4449	nummod	0.02,%
R3002	T4449	T4445	compound	%,solution
R3003	T4450	T4451	compound	thioflavin,S
R3004	T4451	T4445	compound	S,solution
R3005	T4452	T4451	punct	-,S
R3006	T4453	T4454	punct	(,Schmid
R3007	T4454	T4445	parataxis	Schmid,solution
R3008	T4455	T4456	compound	Chroma,Gesellschaft
R3009	T4456	T4454	compound	Gesellschaft,Schmid
R3010	T4457	T4456	punct	-,Gesellschaft
R3011	T4458	T4454	punct	", ",Schmid
R3012	T4459	T4454	npadvmod	Kongen,Schmid
R3013	T4460	T4454	punct	", ",Schmid
R3014	T4461	T4454	npadvmod	Germany,Schmid
R3015	T4462	T4454	punct	),Schmid
R3016	T4463	T4437	prep	for,placed
R3017	T4464	T4465	nummod	8,min
R3018	T4465	T4463	pobj	min,for
R3019	T4466	T4437	punct	.,placed
R3020	T4468	T4469	amod	Excess,stain
R3021	T4469	T4470	nsubjpass	stain,removed
R3022	T4471	T4470	auxpass	was,removed
R3023	T4472	T4470	agent	by,removed
R3024	T4473	T4474	nummod	two,rinses
R3025	T4474	T4472	pobj	rinses,by
R3026	T4475	T4474	amod	brief,rinses
R3027	T4476	T4474	prep	in,rinses
R3028	T4477	T4478	nummod	80,%
R3029	T4478	T4479	compound	%,ethanol
R3030	T4479	T4476	pobj	ethanol,in
R3031	T4480	T4474	punct	", ",rinses
R3032	T4481	T4482	advmod	then,two
R3033	T4482	T4474	appos	two,rinses
R3034	T4483	T4482	prep	in,two
R3035	T4484	T4485	amod	distilled,water
R3036	T4485	T4483	pobj	water,in
R3037	T4486	T4474	punct	", ",rinses
R3038	T4487	T4488	prep	after,finished
R3039	T4488	T4474	relcl	finished,rinses
R3040	T4489	T4487	pobj	which,after
R3041	T4490	T4488	nsubjpass	slides,finished
R3042	T4491	T4488	auxpass	were,finished
R3043	T4492	T4488	prep	in,finished
R3044	T4493	T4494	amod	aqueous,medium
R3045	T4494	T4492	pobj	medium,in
R3046	T4495	T4494	compound	mounting,medium
R3047	T4496	T4488	prep	for,finished
R3048	T4497	T4498	compound	florescence,photomicrography
R3049	T4498	T4496	pobj	photomicrography,for
R3050	T4499	T4470	punct	.,removed
R3051	T4502	T4501	punct	", ",Ubiquitin
R3052	T4503	T4504	amod	glial,protein
R3053	T4504	T4501	conj	protein,Ubiquitin
R3054	T4505	T4504	amod	fibrillary,protein
R3055	T4506	T4504	amod	acidic,protein
R3056	T4507	T4504	punct	", ",protein
R3057	T4508	T4504	cc	and,protein
R3058	T4509	T4510	compound	Aβ,immunohistochemistry
R3059	T4510	T4504	conj	immunohistochemistry,protein
R3060	T4512	T4513	amod	Prior,to
R3061	T4513	T4514	prep	to,deparaffinized
R3062	T4515	T4513	pobj	immunostaining,to
R3063	T4516	T4514	punct	", ",deparaffinized
R3064	T4517	T4514	nsubjpass	slides,deparaffinized
R3065	T4518	T4514	auxpass	were,deparaffinized
R3066	T4519	T4514	prep	by,deparaffinized
R3067	T4520	T4521	compound	oven,heating
R3068	T4521	T4519	pobj	heating,by
R3069	T4522	T4514	advcl	followed,deparaffinized
R3070	T4523	T4522	agent	by,followed
R3071	T4524	T4523	pobj	immersion,by
R3072	T4525	T4524	prep	in,immersion
R3073	T4526	T4525	pobj	xylene,in
R3074	T4527	T4514	punct	.,deparaffinized
R3075	T4529	T4530	prep	After,quenched
R3076	T4531	T4529	pobj	rehydration,After
R3077	T4532	T4531	prep	through,rehydration
R3078	T4533	T4534	amod	graded,alcohols
R3079	T4534	T4532	pobj	alcohols,through
R3080	T4535	T4531	prep	into,rehydration
R3081	T4536	T4537	compound	tap,water
R3082	T4537	T4535	pobj	water,into
R3083	T4538	T4530	punct	", ",quenched
R3084	T4539	T4540	amod	endogenous,activity
R3085	T4540	T4530	nsubjpass	activity,quenched
R3086	T4541	T4540	compound	peroxidase,activity
R3087	T4542	T4530	auxpass	was,quenched
R3088	T4543	T4530	agent	by,quenched
R3089	T4544	T4543	pobj	incubation,by
R3090	T4545	T4544	prep	with,incubation
R3091	T4546	T4547	nummod	3,%
R3092	T4547	T4548	compound	%,peroxide
R3093	T4548	T4545	pobj	peroxide,with
R3094	T4549	T4548	compound	hydrogen,peroxide
R3095	T4550	T4544	prep	in,incubation
R3096	T4551	T4550	pobj	methanol,in
R3097	T4552	T4530	punct	.,quenched
R3098	T4554	T4555	nsubjpass	Slides,microwaved
R3099	T4556	T4555	auxpass	were,microwaved
R3100	T4557	T4555	prep	for,microwaved
R3101	T4558	T4559	quantmod	5,7
R3102	T4559	T4561	nummod	7,min
R3103	T4560	T4559	punct	–,7
R3104	T4561	T4557	pobj	min,for
R3105	T4562	T4555	prep	in,microwaved
R3106	T4563	T4562	pobj	water,in
R3107	T4564	T4555	punct	", ",microwaved
R3108	T4565	T4555	dep	cooled,microwaved
R3109	T4566	T4565	prep	for,cooled
R3110	T4567	T4568	nummod	5,min
R3111	T4568	T4566	pobj	min,for
R3112	T4569	T4555	punct	", ",microwaved
R3113	T4570	T4571	advmod	then,washed
R3114	T4571	T4555	dep	washed,microwaved
R3115	T4572	T4571	prep	in,washed
R3116	T4573	T4572	pobj	TBS,in
R3117	T4574	T4555	punct	.,microwaved
R3118	T4576	T4577	amod	Nonspecific,staining
R3119	T4577	T4578	nsubjpass	staining,blocked
R3120	T4579	T4578	auxpass	was,blocked
R3121	T4580	T4578	prep	for,blocked
R3122	T4581	T4582	nummod	1,h
R3123	T4582	T4580	pobj	h,for
R3124	T4583	T4578	prep	with,blocked
R3125	T4584	T4585	nummod	3,%
R3126	T4585	T4586	nmod	%,serum
R3127	T4586	T4583	pobj	serum,with
R3128	T4587	T4586	amod	normal,serum
R3129	T4588	T4586	compound	goat,serum
R3130	T4589	T4586	cc	and,serum
R3131	T4590	T4591	nummod	0.1,%
R3132	T4591	T4592	compound	%,X
R3133	T4592	T4586	conj	X,serum
R3134	T4593	T4592	compound	Triton,X
R3135	T4594	T4592	punct	-,X
R3136	T4595	T4592	nummod	100,X
R3137	T4596	T4586	prep	in,serum
R3138	T4597	T4596	pobj	TBS,in
R3139	T4598	T4578	punct	.,blocked
R3140	T4600	T4601	nsubjpass	Slides,placed
R3141	T4602	T4601	auxpass	were,placed
R3142	T4603	T4601	advmod	then,placed
R3143	T4604	T4601	prep	into,placed
R3144	T4605	T4606	amod	primary,antibody
R3145	T4606	T4604	pobj	antibody,into
R3146	T4607	T4608	punct	(,antibodies
R3147	T4608	T4606	parataxis	antibodies,antibody
R3148	T4609	T4610	nmod	rabbit,antibody
R3149	T4610	T4608	dep	antibody,antibodies
R3150	T4611	T4610	amod	anti-Aβ,antibody
R3151	T4612	T4610	nmod	peptide,antibody
R3152	T4613	T4610	amod	polyclonal,antibody
R3153	T4614	T4610	punct	", ",antibody
R3154	T4615	T4616	compound	Zymed,Laboratories
R3155	T4616	T4610	npadvmod	Laboratories,antibody
R3156	T4617	T4610	punct	", ",antibody
R3157	T4618	T4619	compound	South,Francisco
R3158	T4619	T4610	npadvmod	Francisco,antibody
R3159	T4620	T4619	compound	San,Francisco
R3160	T4621	T4610	punct	", ",antibody
R3161	T4622	T4610	npadvmod	California,antibody
R3162	T4623	T4610	punct	", ",antibody
R3163	T4624	T4625	compound	United,States
R3164	T4625	T4610	npadvmod	States,antibody
R3165	T4626	T4608	punct	;,antibodies
R3166	T4627	T4608	nmod	rabbit,antibodies
R3167	T4628	T4627	amod	anti-ubiquitin,rabbit
R3168	T4629	T4627	cc	and,rabbit
R3169	T4630	T4627	conj	rabbit,rabbit
R3170	T4631	T4630	amod	anti–glial,rabbit
R3171	T4632	T4633	amod	fibrillary,protein
R3172	T4633	T4608	nmod	protein,antibodies
R3173	T4634	T4633	amod	acidic,protein
R3174	T4635	T4633	punct	(,protein
R3175	T4636	T4633	appos	GFAP,protein
R3176	T4637	T4608	punct	),antibodies
R3177	T4638	T4608	amod	polyclonal,antibodies
R3178	T4639	T4608	punct	", ",antibodies
R3179	T4640	T4608	npadvmod	Dako,antibodies
R3180	T4641	T4608	punct	", ",antibodies
R3181	T4642	T4608	npadvmod	Carpinteria,antibodies
R3182	T4643	T4608	punct	", ",antibodies
R3183	T4644	T4608	npadvmod	California,antibodies
R3184	T4645	T4608	punct	", ",antibodies
R3185	T4646	T4647	compound	United,States
R3186	T4647	T4608	npadvmod	States,antibodies
R3187	T4648	T4608	punct	),antibodies
R3188	T4649	T4606	acl	diluted,antibody
R3189	T4650	T4649	npadvmod	1,diluted
R3190	T4651	T4652	punct	:,500
R3191	T4652	T4650	prep	500,1
R3192	T4653	T4649	prep	in,diluted
R3193	T4654	T4653	pobj	TBS,in
R3194	T4655	T4649	prep	with,diluted
R3195	T4656	T4657	nummod	2,%
R3196	T4657	T4658	nmod	%,serum
R3197	T4658	T4655	pobj	serum,with
R3198	T4659	T4658	amod	normal,serum
R3199	T4660	T4658	compound	goat,serum
R3200	T4661	T4601	cc	and,placed
R3201	T4662	T4601	conj	incubated,placed
R3202	T4663	T4662	advmod	overnight,incubated
R3203	T4664	T4662	prep	at,incubated
R3204	T4665	T4666	compound	room,temperature
R3205	T4666	T4664	pobj	temperature,at
R3206	T4667	T4601	punct	.,placed
R3207	T4669	T4670	prep	After,incubated
R3208	T4671	T4672	auxpass	being,washed
R3209	T4672	T4669	pcomp	washed,After
R3210	T4673	T4672	prep	of,washed
R3211	T4674	T4675	amod	excess,antibody
R3212	T4675	T4673	pobj	antibody,of
R3213	T4676	T4675	amod	primary,antibody
R3214	T4677	T4672	prep	with,washed
R3215	T4678	T4679	amod	several,changes
R3216	T4679	T4677	pobj	changes,with
R3217	T4680	T4679	prep	of,changes
R3218	T4681	T4680	pobj	TBS,of
R3219	T4682	T4670	punct	", ",incubated
R3220	T4683	T4670	nsubjpass	slides,incubated
R3221	T4684	T4670	auxpass	were,incubated
R3222	T4685	T4670	prep	with,incubated
R3223	T4686	T4687	preconj	either,system
R3224	T4687	T4685	pobj	system,with
R3225	T4688	T4687	det	the,system
R3226	T4689	T4690	nmod	Vectastain,Elite
R3227	T4690	T4687	nmod	Elite,system
R3228	T4691	T4687	amod	anti-rabbit,system
R3229	T4692	T4687	amod	secondary,system
R3230	T4693	T4694	punct	(,Laboratories
R3231	T4694	T4687	parataxis	Laboratories,system
R3232	T4695	T4694	amod	anti-Aβ,Laboratories
R3233	T4696	T4694	punct	;,Laboratories
R3234	T4697	T4694	compound	Vector,Laboratories
R3235	T4698	T4694	punct	", ",Laboratories
R3236	T4699	T4694	npadvmod	Burlingame,Laboratories
R3237	T4700	T4694	punct	", ",Laboratories
R3238	T4701	T4694	npadvmod	California,Laboratories
R3239	T4702	T4694	punct	", ",Laboratories
R3240	T4703	T4704	compound	United,States
R3241	T4704	T4694	npadvmod	States,Laboratories
R3242	T4705	T4694	punct	),Laboratories
R3243	T4706	T4687	cc	or,system
R3244	T4707	T4708	nmod	peroxidase,reagents
R3245	T4708	T4687	conj	reagents,system
R3246	T4709	T4707	punct	/,peroxidase
R3247	T4710	T4707	amod	anti-peroxidase,peroxidase
R3248	T4711	T4712	punct	(,Monoclonals
R3249	T4712	T4708	parataxis	Monoclonals,reagents
R3250	T4713	T4712	amod	anti-ubitquitin,Monoclonals
R3251	T4714	T4713	cc	and,anti-ubitquitin
R3252	T4715	T4713	conj	anti-GFAP,anti-ubitquitin
R3253	T4716	T4712	punct	;,Monoclonals
R3254	T4717	T4712	compound	Sternberger,Monoclonals
R3255	T4718	T4712	punct	", ",Monoclonals
R3256	T4719	T4712	npadvmod	Lutherville,Monoclonals
R3257	T4720	T4712	punct	", ",Monoclonals
R3258	T4721	T4712	npadvmod	Maryland,Monoclonals
R3259	T4722	T4712	punct	", ",Monoclonals
R3260	T4723	T4724	compound	United,States
R3261	T4724	T4712	npadvmod	States,Monoclonals
R3262	T4725	T4712	punct	),Monoclonals
R3263	T4726	T4670	prep	according,incubated
R3264	T4727	T4726	prep	to,according
R3265	T4728	T4729	det	the,manufacturers
R3266	T4729	T4730	poss	manufacturers,directions
R3267	T4730	T4727	pobj	directions,to
R3268	T4731	T4729	case	',manufacturers
R3269	T4732	T4670	punct	.,incubated
R3270	T4734	T4735	compound	Antibody,binding
R3271	T4735	T4736	nsubjpass	binding,visualized
R3272	T4737	T4736	auxpass	was,visualized
R3273	T4738	T4736	prep	with,visualized
R3274	T4739	T4738	pobj	diaminobenzidene,with
R3275	T4740	T4736	punct	", ",visualized
R3276	T4741	T4736	cc	and,visualized
R3277	T4742	T4743	nsubjpass	sections,counterstained
R3278	T4743	T4736	conj	counterstained,visualized
R3279	T4744	T4743	auxpass	were,counterstained
R3280	T4745	T4743	prep	with,counterstained
R3281	T4746	T4745	pobj	hematoxylin,with
R3282	T4747	T4743	punct	.,counterstained
R3283	T4787	T4788	compound	Filter,Assay
R3284	T4789	T4788	compound	Trap,Assay
R3285	T4791	T4792	det	An,aliquot
R3286	T4792	T4793	nsubjpass	aliquot,solubilized
R3287	T4794	T4792	prep	of,aliquot
R3288	T4795	T4796	det	each,homogenate
R3289	T4796	T4794	pobj	homogenate,of
R3290	T4797	T4796	amod	cortical,homogenate
R3291	T4798	T4796	acl	used,homogenate
R3292	T4799	T4798	prep	for,used
R3293	T4800	T4801	compound	Western,blotting
R3294	T4801	T4799	pobj	blotting,for
R3295	T4802	T4798	advmod	above,used
R3296	T4803	T4793	auxpass	was,solubilized
R3297	T4804	T4793	advmod	partially,solubilized
R3298	T4805	T4793	prep	by,solubilized
R3299	T4806	T4807	det	the,addition
R3300	T4807	T4805	pobj	addition,by
R3301	T4808	T4807	prep	of,addition
R3302	T4809	T4808	pobj	SDS,of
R3303	T4810	T4793	prep	to,solubilized
R3304	T4811	T4812	det	a,concentration
R3305	T4812	T4810	pobj	concentration,to
R3306	T4813	T4812	amod	final,concentration
R3307	T4814	T4812	prep	of,concentration
R3308	T4815	T4816	nummod	1,%
R3309	T4816	T4814	pobj	%,of
R3310	T4817	T4793	punct	.,solubilized
R3311	T4819	T4820	amod	Serial,dilutions
R3312	T4820	T4824	nsubjpass	dilutions,made
R3313	T4821	T4820	nummod	1,dilutions
R3314	T4822	T4823	punct	:,2
R3315	T4823	T4821	prep	2,1
R3316	T4825	T4824	auxpass	were,made
R3317	T4826	T4824	prep	with,made
R3318	T4827	T4828	nummod	1,PBS
R3319	T4828	T4826	pobj	PBS,with
R3320	T4829	T4827	punct	×,1
R3321	T4830	T4828	punct	/,PBS
R3322	T4831	T4832	nummod	1,%
R3323	T4832	T4833	compound	%,SDS
R3324	T4833	T4828	appos	SDS,PBS
R3325	T4834	T4824	punct	", ",made
R3326	T4835	T4824	cc	and,made
R3327	T4836	T4837	nummod	100,μl
R3328	T4837	T4838	nsubjpass	μl,filtered
R3329	T4838	T4824	conj	filtered,made
R3330	T4839	T4837	prep	of,μl
R3331	T4840	T4841	det	each,dilution
R3332	T4841	T4839	pobj	dilution,of
R3333	T4842	T4838	auxpass	was,filtered
R3334	T4843	T4838	advmod	then,filtered
R3335	T4844	T4838	dep	vacuum,filtered
R3336	T4845	T4838	punct	-,filtered
R3337	T4846	T4838	prep	through,filtered
R3338	T4847	T4848	det	a,membrane
R3339	T4848	T4846	pobj	membrane,through
R3340	T4849	T4848	amod	pre-wet,membrane
R3341	T4850	T4851	nummod	0.22,μm
R3342	T4851	T4848	compound	μm,membrane
R3343	T4852	T4851	punct	-,μm
R3344	T4853	T4848	compound	cellulose,membrane
R3345	T4854	T4848	compound	acetate,membrane
R3346	T4855	T4856	punct	(,Schleicher
R3347	T4856	T4848	parataxis	Schleicher,membrane
R3348	T4857	T4856	cc	and,Schleicher
R3349	T4858	T4856	conj	Schuell,Schleicher
R3350	T4859	T4856	punct	),Schleicher
R3351	T4860	T4861	punct	[,22
R3352	T4861	T4838	parataxis	22,filtered
R3353	T4862	T4861	punct	],22
R3354	T4863	T4838	punct	.,filtered
R3355	T4865	T4866	det	Each,well
R3356	T4866	T4867	nsubjpass	well,washed
R3357	T4868	T4867	auxpass	was,washed
R3358	T4869	T4870	amod	several,times
R3359	T4870	T4867	dobj	times,washed
R3360	T4871	T4867	prep	with,washed
R3361	T4872	T4871	pobj	PBS,with
R3362	T4873	T4867	punct	", ",washed
R3363	T4874	T4875	prep	after,incubated
R3364	T4875	T4867	advcl	incubated,washed
R3365	T4876	T4874	pobj	which,after
R3366	T4877	T4875	nsubjpass	blots,incubated
R3367	T4878	T4875	auxpass	were,incubated
R3368	T4879	T4875	advmod	overnight,incubated
R3369	T4880	T4875	prep	with,incubated
R3370	T4881	T4882	amod	polyclonal,antibody
R3371	T4882	T4880	pobj	antibody,with
R3372	T4883	T4882	amod	anti-Aβ,antibody
R3373	T4884	T4885	punct	(,Laboratories
R3374	T4885	T4882	parataxis	Laboratories,antibody
R3375	T4886	T4885	compound	Zymed,Laboratories
R3376	T4887	T4885	punct	),Laboratories
R3377	T4888	T4882	acl	diluted,antibody
R3378	T4889	T4888	npadvmod	1,diluted
R3379	T4890	T4891	punct	:,600
R3380	T4891	T4889	prep	600,1
R3381	T4892	T4888	prep	in,diluted
R3382	T4893	T4894	det	a,solution
R3383	T4894	T4892	pobj	solution,in
R3384	T4895	T4894	compound	blocking,solution
R3385	T4896	T4894	prep	of,solution
R3386	T4897	T4898	nummod	1,TBS
R3387	T4898	T4900	nmod	TBS,powder
R3388	T4899	T4897	punct	×,1
R3389	T4900	T4896	pobj	powder,of
R3390	T4901	T4898	punct	/,TBS
R3391	T4902	T4903	nummod	5,%
R3392	T4903	T4904	nmod	%,milk
R3393	T4904	T4898	appos	milk,TBS
R3394	T4905	T4904	amod	nonfat,milk
R3395	T4906	T4904	amod	dry,milk
R3396	T4907	T4867	punct	.,washed
R3397	T4909	T4910	prep	After,incubated
R3398	T4911	T4909	pcomp	washing,After
R3399	T4912	T4913	det	the,blots
R3400	T4913	T4911	dobj	blots,washing
R3401	T4914	T4915	nummod	three,times
R3402	T4915	T4911	npadvmod	times,washing
R3403	T4916	T4911	prep	for,washing
R3404	T4917	T4918	nummod	10,min
R3405	T4918	T4916	pobj	min,for
R3406	T4919	T4918	appos	each,min
R3407	T4920	T4911	prep	in,washing
R3408	T4921	T4922	nummod	1,TBS
R3409	T4922	T4920	pobj	TBS,in
R3410	T4923	T4921	punct	×,1
R3411	T4924	T4922	punct	/,TBS
R3412	T4925	T4926	nummod	0.1,%
R3413	T4926	T4927	compound	%,Tween
R3414	T4927	T4922	appos	Tween,TBS
R3415	T4928	T4927	punct	-,Tween
R3416	T4929	T4927	nummod	20,Tween
R3417	T4930	T4910	punct	", ",incubated
R3418	T4931	T4932	det	the,membrane
R3419	T4932	T4910	nsubjpass	membrane,incubated
R3420	T4933	T4910	auxpass	was,incubated
R3421	T4934	T4910	prep	for,incubated
R3422	T4935	T4936	nummod	1,h
R3423	T4936	T4934	pobj	h,for
R3424	T4937	T4910	prep	with,incubated
R3425	T4938	T4939	npadvmod	HRP,conjugated
R3426	T4939	T4941	amod	conjugated,A
R3427	T4940	T4939	punct	-,conjugated
R3428	T4941	T4937	pobj	A,with
R3429	T4942	T4941	compound	protein,A
R3430	T4943	T4944	punct	(,Sigma
R3431	T4944	T4941	parataxis	Sigma,A
R3432	T4945	T4944	punct	),Sigma
R3433	T4946	T4941	acl	diluted,A
R3434	T4947	T4946	dobj	1,diluted
R3435	T4948	T4949	punct	:,"5,000"
R3436	T4949	T4947	prep	"5,000",1
R3437	T4950	T4946	prep	in,diluted
R3438	T4951	T4952	compound	blocking,solution
R3439	T4952	T4950	pobj	solution,in
R3440	T4953	T4910	punct	.,incubated
R3441	T4955	T4956	det	The,membranes
R3442	T4956	T4957	nsubjpass	membranes,washed
R3443	T4958	T4957	auxpass	were,washed
R3444	T4959	T4957	advmod	again,washed
R3445	T4960	T4961	nummod	three,times
R3446	T4961	T4957	npadvmod	times,washed
R3447	T4962	T4957	prep	with,washed
R3448	T4963	T4964	nummod	1,TBS
R3449	T4964	T4962	pobj	TBS,with
R3450	T4965	T4963	punct	×,1
R3451	T4966	T4964	punct	/,TBS
R3452	T4967	T4968	nummod	0.1,%
R3453	T4968	T4969	compound	%,Tween
R3454	T4969	T4964	appos	Tween,TBS
R3455	T4970	T4969	punct	-,Tween
R3456	T4971	T4969	nummod	20,Tween
R3457	T4972	T4957	punct	", ",washed
R3458	T4973	T4974	mark	before,detected
R3459	T4974	T4957	advcl	detected,washed
R3460	T4975	T4976	compound	antibody,binding
R3461	T4976	T4974	nsubjpass	binding,detected
R3462	T4977	T4974	auxpass	was,detected
R3463	T4978	T4974	prep	with,detected
R3464	T4979	T4980	amod	enhanced,chemiluminescence
R3465	T4980	T4978	pobj	chemiluminescence,with
R3466	T4981	T4982	punct	(,PerkinElmer
R3467	T4982	T4974	parataxis	PerkinElmer,detected
R3468	T4983	T4982	punct	", ",PerkinElmer
R3469	T4984	T4982	npadvmod	Boston,PerkinElmer
R3470	T4985	T4982	punct	", ",PerkinElmer
R3471	T4986	T4982	npadvmod	Massachusetts,PerkinElmer
R3472	T4987	T4982	punct	", ",PerkinElmer
R3473	T4988	T4989	compound	United,States
R3474	T4989	T4982	npadvmod	States,PerkinElmer
R3475	T4990	T4982	punct	),PerkinElmer
R3476	T4991	T4957	punct	.,washed
R3477	T4993	T4994	amod	Digital,images
R3478	T4994	T4995	nsubjpass	images,captured
R3479	T4996	T4994	prep	of,images
R3480	T4997	T4998	det	each,blot
R3481	T4998	T4996	pobj	blot,of
R3482	T4999	T4995	auxpass	were,captured
R3483	T5000	T4995	prep	with,captured
R3484	T5001	T5002	det	a,system
R3485	T5002	T5000	pobj	system,with
R3486	T5003	T5004	amod	Molecular,Imager
R3487	T5004	T5005	compound	Imager,FX
R3488	T5005	T5002	compound	FX,system
R3489	T5006	T5007	compound	gel,documentation
R3490	T5007	T5002	compound	documentation,system
R3491	T5008	T4995	punct	", ",captured
R3492	T5009	T4995	cc	and,captured
R3493	T5010	T5011	det	the,intensity
R3494	T5011	T5012	nsubjpass	intensity,quantified
R3495	T5012	T4995	conj	quantified,captured
R3496	T5013	T5011	prep	of,intensity
R3497	T5014	T5015	compound	Aβ,staining
R3498	T5015	T5013	pobj	staining,of
R3499	T5016	T5012	auxpass	was,quantified
R3500	T5017	T5012	advcl	using,quantified
R3501	T5018	T5019	nmod	Quantity,software
R3502	T5019	T5017	dobj	software,using
R3503	T5020	T5018	nummod	One,Quantity
R3504	T5021	T5022	compound	image,analysis
R3505	T5022	T5019	compound	analysis,software
R3506	T5023	T5012	punct	.,quantified
R3507	T5075	T5076	compound	Aβ,ELISA
R3508	T5078	T5079	det	An,aliquot
R3509	T5079	T5080	nsubjpass	aliquot,subjected
R3510	T5081	T5079	prep	of,aliquot
R3511	T5082	T5083	amod	cortical,homogenate
R3512	T5083	T5081	pobj	homogenate,of
R3513	T5084	T5083	acl	generated,homogenate
R3514	T5085	T5084	prep	for,generated
R3515	T5086	T5087	compound	Western,analysis
R3516	T5087	T5085	pobj	analysis,for
R3517	T5088	T5087	acl	described,analysis
R3518	T5089	T5088	advmod	above,described
R3519	T5090	T5080	auxpass	was,subjected
R3520	T5091	T5080	prep	to,subjected
R3521	T5092	T5093	det	a,extraction
R3522	T5093	T5091	pobj	extraction,to
R3523	T5094	T5095	nummod	three,step
R3524	T5095	T5093	nmod	step,extraction
R3525	T5096	T5095	punct	-,step
R3526	T5097	T5093	amod	sequential,extraction
R3527	T5098	T5080	advcl	using,subjected
R3528	T5099	T5098	dobj	PBS,using
R3529	T5100	T5099	punct	", ",PBS
R3530	T5101	T5102	nummod	2,%
R3531	T5102	T5103	compound	%,SDS
R3532	T5103	T5099	conj	SDS,PBS
R3533	T5104	T5103	punct	", ",SDS
R3534	T5105	T5103	cc	and,SDS
R3535	T5106	T5107	nummod	70,%
R3536	T5107	T5103	conj	%,SDS
R3537	T5108	T5109	amod	formic,acid
R3538	T5109	T5107	appos	acid,%
R3539	T5110	T5109	punct	(,acid
R3540	T5111	T5109	appos	FA,acid
R3541	T5112	T5080	punct	),subjected
R3542	T5113	T5080	punct	.,subjected
R3543	T5115	T5116	prep	At,sonicated
R3544	T5117	T5118	det	each,step
R3545	T5118	T5115	pobj	step,At
R3546	T5119	T5116	punct	", ",sonicated
R3547	T5120	T5121	det	the,sample
R3548	T5121	T5116	nsubjpass	sample,sonicated
R3549	T5122	T5116	auxpass	was,sonicated
R3550	T5123	T5116	prep	in,sonicated
R3551	T5124	T5125	amod	appropriate,buffer
R3552	T5125	T5123	pobj	buffer,in
R3553	T5126	T5116	cc	and,sonicated
R3554	T5127	T5116	conj	centrifuged,sonicated
R3555	T5128	T5127	prep	at,centrifuged
R3556	T5129	T5128	pobj	"100,000g",at
R3557	T5130	T5127	prep	for,centrifuged
R3558	T5131	T5132	nummod	30,min
R3559	T5132	T5130	pobj	min,for
R3560	T5133	T5134	punct	(,samples
R3561	T5134	T5132	parataxis	samples,min
R3562	T5135	T5136	quantmod	1,1.5
R3563	T5136	T5139	nummod	1.5,mo
R3564	T5137	T5136	punct	-,1.5
R3565	T5138	T5136	quantmod	to,1.5
R3566	T5139	T5134	compound	mo,samples
R3567	T5140	T5139	punct	-,mo
R3568	T5141	T5134	punct	),samples
R3569	T5142	T5132	cc	or,min
R3570	T5143	T5144	nummod	60,min
R3571	T5144	T5132	conj	min,min
R3572	T5145	T5146	punct	(,samples
R3573	T5146	T5144	parataxis	samples,min
R3574	T5147	T5148	quantmod	6,12
R3575	T5148	T5151	nummod	12,mo
R3576	T5149	T5148	punct	-,12
R3577	T5150	T5148	quantmod	to,12
R3578	T5151	T5146	compound	mo,samples
R3579	T5152	T5151	punct	-,mo
R3580	T5153	T5146	punct	),samples
R3581	T5154	T5127	prep	at,centrifuged
R3582	T5155	T5156	nummod	4,°C
R3583	T5156	T5154	pobj	°C,at
R3584	T5157	T5158	mark	as,described
R3585	T5158	T5127	advcl	described,centrifuged
R3586	T5159	T5158	advmod	previously,described
R3587	T5160	T5161	punct	[,23
R3588	T5161	T5127	parataxis	23,centrifuged
R3589	T5162	T5163	punct	–,25
R3590	T5163	T5161	prep	25,23
R3591	T5164	T5161	punct	],23
R3592	T5165	T5116	punct	.,sonicated
R3593	T5167	T5168	det	The,supernatant
R3594	T5168	T5169	nsubjpass	supernatant,removed
R3595	T5170	T5169	auxpass	was,removed
R3596	T5171	T5169	prep	for,removed
R3597	T5172	T5171	pobj	analysis,for
R3598	T5173	T5169	punct	", ",removed
R3599	T5174	T5169	cc	and,removed
R3600	T5175	T5176	det	the,pellet
R3601	T5176	T5177	nsubjpass	pellet,sonicated
R3602	T5177	T5169	conj	sonicated,removed
R3603	T5178	T5177	auxpass	was,sonicated
R3604	T5179	T5177	prep	in,sonicated
R3605	T5180	T5181	det	the,solution
R3606	T5181	T5179	pobj	solution,in
R3607	T5182	T5181	amod	next,solution
R3608	T5183	T5177	prep	in,sonicated
R3609	T5184	T5183	pobj	sequence,in
R3610	T5185	T5177	punct	.,sonicated
R3611	T5187	T5188	det	The,extracts
R3612	T5188	T5192	nsubjpass	extracts,diluted
R3613	T5189	T5190	nummod	2,%
R3614	T5190	T5188	compound	%,extracts
R3615	T5191	T5188	compound	SDS,extracts
R3616	T5193	T5192	auxpass	were,diluted
R3617	T5194	T5192	prep	in,diluted
R3618	T5195	T5196	compound	EC,buffer
R3619	T5196	T5194	pobj	buffer,in
R3620	T5197	T5192	punct	", ",diluted
R3621	T5198	T5192	cc	and,diluted
R3622	T5199	T5200	det	the,extracts
R3623	T5200	T5202	nsubj	extracts,neutralized
R3624	T5201	T5200	compound	FA,extracts
R3625	T5202	T5192	conj	neutralized,diluted
R3626	T5203	T5202	prep	with,neutralized
R3627	T5204	T5205	compound	1M,buffer
R3628	T5205	T5203	pobj	buffer,with
R3629	T5206	T5205	compound	Tris,buffer
R3630	T5207	T5205	punct	-,buffer
R3631	T5208	T5205	compound	phosphate,buffer
R3632	T5209	T5210	punct	(,pH
R3633	T5210	T5205	parataxis	pH,buffer
R3634	T5211	T5210	nummod	11,pH
R3635	T5212	T5210	punct	),pH
R3636	T5213	T5214	advmod	then,diluted
R3637	T5214	T5202	dep	diluted,neutralized
R3638	T5215	T5214	prep	with,diluted
R3639	T5216	T5217	compound	EC,buffer
R3640	T5217	T5215	pobj	buffer,with
R3641	T5218	T5219	amod	prior,to
R3642	T5219	T5214	prep	to,diluted
R3643	T5220	T5219	pobj	testing,to
R3644	T5221	T5202	punct	.,neutralized
R3645	T5223	T5224	amod	Human,Aβ
R3646	T5224	T5225	nsubjpass	Aβ,measured
R3647	T5226	T5225	auxpass	was,measured
R3648	T5227	T5225	prep	in,measured
R3649	T5228	T5229	det	each,fraction
R3650	T5229	T5227	pobj	fraction,in
R3651	T5230	T5225	advcl	using,measured
R3652	T5231	T5230	dobj	BAN50,using
R3653	T5232	T5230	prep	for,using
R3654	T5233	T5232	pobj	capture,for
R3655	T5234	T5235	punct	(,16
R3656	T5235	T5233	parataxis	16,capture
R3657	T5236	T5235	compound	epitope,16
R3658	T5237	T5235	compound	Aβ1,16
R3659	T5238	T5235	punct	–,16
R3660	T5239	T5235	punct	),16
R3661	T5240	T5230	cc	and,using
R3662	T5241	T5230	conj	BA27,using
R3663	T5242	T5241	cc	and,BA27
R3664	T5243	T5241	conj	BC05,BA27
R3665	T5244	T5241	prep	for,BA27
R3666	T5245	T5244	pobj	detection,for
R3667	T5246	T5247	punct	(,Aβ40
R3668	T5247	T5245	parataxis	Aβ40,detection
R3669	T5248	T5247	cc	and,Aβ40
R3670	T5249	T5247	conj	Aβ42,Aβ40
R3671	T5250	T5247	punct	", ",Aβ40
R3672	T5251	T5247	advmod	respectively,Aβ40
R3673	T5252	T5247	punct	),Aβ40
R3674	T5253	T5254	punct	(,Industries
R3675	T5254	T5245	parataxis	Industries,detection
R3676	T5255	T5254	compound	Takeda,Industries
R3677	T5256	T5254	compound	Chemical,Industries
R3678	T5257	T5254	punct	", ",Industries
R3679	T5258	T5254	npadvmod	Osaka,Industries
R3680	T5259	T5254	punct	", ",Industries
R3681	T5260	T5254	npadvmod	Japan,Industries
R3682	T5261	T5254	punct	),Industries
R3683	T5262	T5225	punct	.,measured
R3684	T5264	T5265	amod	Total,Aβ
R3685	T5265	T5266	nsubjpass	Aβ,measured
R3686	T5267	T5268	punct	(,samples
R3687	T5268	T5265	parataxis	samples,Aβ
R3688	T5269	T5268	dep	mouse,samples
R3689	T5270	T5269	punct	+,mouse
R3690	T5271	T5269	amod	human,mouse
R3691	T5272	T5268	punct	;,samples
R3692	T5273	T5274	quantmod	1,1.5
R3693	T5274	T5277	nummod	1.5,mo
R3694	T5275	T5274	punct	-,1.5
R3695	T5276	T5274	quantmod	to,1.5
R3696	T5277	T5268	compound	mo,samples
R3697	T5278	T5277	punct	-,mo
R3698	T5279	T5268	advmod	only,samples
R3699	T5280	T5268	punct	),samples
R3700	T5281	T5266	auxpass	was,measured
R3701	T5282	T5266	prep	in,measured
R3702	T5283	T5284	det	each,fraction
R3703	T5284	T5282	pobj	fraction,in
R3704	T5285	T5266	advcl	using,measured
R3705	T5286	T5285	dobj	BNT77,using
R3706	T5287	T5285	prep	for,using
R3707	T5288	T5287	pobj	capture,for
R3708	T5289	T5290	punct	(,Aβ11
R3709	T5290	T5288	parataxis	Aβ11,capture
R3710	T5291	T5290	compound	epitope,Aβ11
R3711	T5292	T5290	punct	–,Aβ11
R3712	T5293	T5290	nummod	28,Aβ11
R3713	T5294	T5290	punct	),Aβ11
R3714	T5295	T5285	cc	and,using
R3715	T5296	T5285	conj	BA27,using
R3716	T5297	T5296	cc	and,BA27
R3717	T5298	T5296	conj	BC05,BA27
R3718	T5299	T5296	prep	for,BA27
R3719	T5300	T5299	pobj	detection,for
R3720	T5301	T5266	punct	.,measured
R3721	T5303	T5304	det	All,values
R3722	T5304	T5305	nsubjpass	values,calculated
R3723	T5306	T5305	auxpass	were,calculated
R3724	T5307	T5305	prep	as,calculated
R3725	T5308	T5307	pobj	picomoles,as
R3726	T5309	T5308	prep	per,picomoles
R3727	T5310	T5309	pobj	gram,per
R3728	T5311	T5305	prep	based,calculated
R3729	T5312	T5311	prep	on,based
R3730	T5313	T5314	det	the,weight
R3731	T5314	T5312	pobj	weight,on
R3732	T5315	T5314	amod	initial,weight
R3733	T5316	T5314	prep	of,weight
R3734	T5317	T5318	amod	cortical,tissue
R3735	T5318	T5316	pobj	tissue,of
R3736	T5319	T5305	punct	.,calculated
R3737	T5339	T5340	compound	Activity,Monitoring
R3738	T5342	T5343	amod	Daily,activity
R3739	T5343	T5345	nsubjpass	activity,studied
R3740	T5344	T5343	amod	basal,activity
R3741	T5346	T5345	auxpass	was,studied
R3742	T5347	T5345	prep	in,studied
R3743	T5348	T5349	nummod	28,mice
R3744	T5349	T5347	pobj	mice,in
R3745	T5350	T5351	nmod	CaMKIIα,ind
R3746	T5351	T5349	nmod	ind,mice
R3747	T5352	T5351	punct	-,ind
R3748	T5353	T5351	nmod	tTA,ind
R3749	T5354	T5351	punct	×,ind
R3750	T5355	T5351	nmod	tet,ind
R3751	T5356	T5351	punct	-,ind
R3752	T5357	T5351	nmod	APPswe,ind
R3753	T5358	T5351	punct	/,ind
R3754	T5359	T5349	nmod	line,mice
R3755	T5360	T5359	nummod	107,line
R3756	T5361	T5362	quantmod	between,4
R3757	T5362	T5363	nummod	4,mo
R3758	T5363	T5349	npadvmod	mo,mice
R3759	T5364	T5362	cc	and,4
R3760	T5365	T5362	conj	5,4
R3761	T5366	T5363	prep	of,mo
R3762	T5367	T5366	pobj	age,of
R3763	T5368	T5345	punct	.,studied
R3764	T5370	T5371	nsubjpass	Animals,separated
R3765	T5372	T5371	auxpass	were,separated
R3766	T5373	T5371	prep	into,separated
R3767	T5374	T5375	amod	individual,cages
R3768	T5375	T5373	pobj	cages,into
R3769	T5376	T5377	advmod	immediately,before
R3770	T5377	T5371	prep	before,separated
R3771	T5378	T5379	det	the,start
R3772	T5379	T5377	pobj	start,before
R3773	T5380	T5379	prep	of,start
R3774	T5381	T5382	det	each,experiment
R3775	T5382	T5380	pobj	experiment,of
R3776	T5383	T5384	punct	(,6
R3777	T5384	T5371	parataxis	6,separated
R3778	T5385	T5384	nsubj	n,6
R3779	T5386	T5384	punct	=,6
R3780	T5387	T5384	quantmod	3,6
R3781	T5388	T5384	punct	–,6
R3782	T5389	T5384	prep	per,6
R3783	T5390	T5389	pobj	genotype,per
R3784	T5391	T5390	amod	untreated,genotype
R3785	T5392	T5384	punct	", ",6
R3786	T5393	T5394	quantmod	2,5
R3787	T5394	T5384	appos	5,6
R3788	T5395	T5394	punct	–,5
R3789	T5396	T5394	prep	per,5
R3790	T5397	T5396	pobj	genotype,per
R3791	T5398	T5399	dep	dox,reared
R3792	T5399	T5397	acl	reared,genotype
R3793	T5400	T5399	punct	-,reared
R3794	T5401	T5384	punct	),6
R3795	T5402	T5371	punct	.,separated
R3796	T5404	T5405	det	The,cages
R3797	T5405	T5406	nsubjpass	cages,placed
R3798	T5407	T5406	auxpass	were,placed
R3799	T5408	T5406	prep	inside,placed
R3800	T5409	T5410	npadvmod	activity,monitoring
R3801	T5410	T5412	amod	monitoring,frames
R3802	T5411	T5410	punct	-,monitoring
R3803	T5412	T5408	pobj	frames,inside
R3804	T5413	T5412	acl	designed,frames
R3805	T5414	T5415	aux	to,count
R3806	T5415	T5413	advcl	count,designed
R3807	T5416	T5417	det	every,time
R3808	T5417	T5415	dobj	time,count
R3809	T5418	T5419	det	the,animal
R3810	T5419	T5420	nsubj	animal,passed
R3811	T5420	T5417	advcl	passed,time
R3812	T5421	T5420	prep	through,passed
R3813	T5422	T5421	pobj	one,through
R3814	T5423	T5422	prep	of,one
R3815	T5424	T5425	nummod	three,photobeams
R3816	T5425	T5423	pobj	photobeams,of
R3817	T5426	T5425	acl	spanning,photobeams
R3818	T5427	T5428	det	the,width
R3819	T5428	T5426	dobj	width,spanning
R3820	T5429	T5428	prep	of,width
R3821	T5430	T5431	det	the,cage
R3822	T5431	T5429	pobj	cage,of
R3823	T5432	T5433	punct	(,Instruments
R3824	T5433	T5420	parataxis	Instruments,passed
R3825	T5434	T5435	compound	San,Diego
R3826	T5435	T5433	compound	Diego,Instruments
R3827	T5436	T5433	punct	", ",Instruments
R3828	T5437	T5438	compound	San,Diego
R3829	T5438	T5433	npadvmod	Diego,Instruments
R3830	T5439	T5433	punct	", ",Instruments
R3831	T5440	T5433	npadvmod	California,Instruments
R3832	T5441	T5433	punct	", ",Instruments
R3833	T5442	T5443	compound	United,States
R3834	T5443	T5433	npadvmod	States,Instruments
R3835	T5444	T5433	punct	),Instruments
R3836	T5445	T5406	punct	.,placed
R3837	T5447	T5448	nsubjpass	Experiments,started
R3838	T5449	T5448	auxpass	were,started
R3839	T5450	T5451	advmod	midway,through
R3840	T5451	T5448	prep	through,started
R3841	T5452	T5453	det	the,phase
R3842	T5453	T5451	pobj	phase,through
R3843	T5454	T5453	compound	light,phase
R3844	T5455	T5453	prep	of,phase
R3845	T5456	T5457	det	the,day
R3846	T5457	T5455	pobj	day,of
R3847	T5458	T5448	punct	", ",started
R3848	T5459	T5448	cc	and,started
R3849	T5460	T5461	nsubjpass	data,collected
R3850	T5461	T5448	conj	collected,started
R3851	T5462	T5461	auxpass	were,collected
R3852	T5463	T5461	prep	in,collected
R3853	T5464	T5465	nummod	1,h
R3854	T5465	T5467	compound	h,bins
R3855	T5466	T5465	punct	-,h
R3856	T5467	T5463	pobj	bins,in
R3857	T5468	T5461	prep	for,collected
R3858	T5469	T5470	det	the,h
R3859	T5470	T5468	pobj	h,for
R3860	T5471	T5470	amod	following,h
R3861	T5472	T5470	nummod	48,h
R3862	T5473	T5461	punct	.,collected
R3863	T5475	T5476	compound	Testing,rooms
R3864	T5476	T5477	nsubjpass	rooms,maintained
R3865	T5478	T5477	auxpass	were,maintained
R3866	T5479	T5477	prep	on,maintained
R3867	T5480	T5481	det	the,cycle
R3868	T5481	T5479	pobj	cycle,on
R3869	T5482	T5481	amod	same,cycle
R3870	T5483	T5484	nummod	13,h
R3871	T5484	T5481	nmod	h,cycle
R3872	T5485	T5486	punct	:,11
R3873	T5486	T5483	prep	11,13
R3874	T5487	T5481	nmod	day,cycle
R3875	T5488	T5489	punct	:,night
R3876	T5489	T5487	prep	night,day
R3877	T5490	T5481	prep	as,cycle
R3878	T5491	T5492	det	the,areas
R3879	T5492	T5490	pobj	areas,as
R3880	T5493	T5492	amod	main,areas
R3881	T5494	T5495	compound	animal,housing
R3882	T5495	T5492	compound	housing,areas
R3883	T5496	T5477	cc	and,maintained
R3884	T5497	T5498	auxpass	were,closed
R3885	T5498	T5477	conj	closed,maintained
R3886	T5499	T5498	prep	to,closed
R3887	T5500	T5499	pobj	entry,to
R3888	T5501	T5498	prep	during,closed
R3889	T5502	T5503	det	the,experiment
R3890	T5503	T5501	pobj	experiment,during
R3891	T5504	T5477	punct	.,maintained
R3892	T5510	T5511	amod	Statistical,Analyses
R3893	T5513	T5514	amod	Statistical,analyses
R3894	T5514	T5515	nsubjpass	analyses,performed
R3895	T5516	T5514	prep	of,analyses
R3896	T5517	T5518	compound	protein,expression
R3897	T5518	T5516	pobj	expression,of
R3898	T5519	T5518	punct	", ",expression
R3899	T5520	T5521	compound	ELISA,data
R3900	T5521	T5518	conj	data,expression
R3901	T5522	T5521	punct	", ",data
R3902	T5523	T5521	cc	and,data
R3903	T5524	T5525	compound	filter,trap
R3904	T5525	T5526	compound	trap,assays
R3905	T5526	T5521	conj	assays,data
R3906	T5527	T5515	auxpass	were,performed
R3907	T5528	T5515	prep	by,performed
R3908	T5529	T5528	pobj	ANOVA,by
R3909	T5530	T5515	prep	with,performed
R3910	T5531	T5532	poss	Tukey,test
R3911	T5532	T5530	pobj	test,with
R3912	T5533	T5531	case	's,Tukey
R3913	T5534	T5532	amod	honest,test
R3914	T5535	T5532	amod	significant,test
R3915	T5536	T5532	nmod	difference,test
R3916	T5537	T5538	nmod	post,hoc
R3917	T5538	T5532	nmod	hoc,test
R3918	T5539	T5538	punct	-,hoc
R3919	T5540	T5532	acl	applied,test
R3920	T5541	T5540	prep	to,applied
R3921	T5542	T5543	amod	significant,effects
R3922	T5543	T5541	pobj	effects,to
R3923	T5544	T5543	amod	main,effects
R3924	T5545	T5543	cc	or,effects
R3925	T5546	T5543	conj	interactions,effects
R3926	T5547	T5548	punct	(,Statistica
R3927	T5548	T5515	parataxis	Statistica,performed
R3928	T5549	T5548	nummod	6.0,Statistica
R3929	T5550	T5548	punct	", ",Statistica
R3930	T5551	T5548	npadvmod	StatSoft,Statistica
R3931	T5552	T5548	punct	", ",Statistica
R3932	T5553	T5548	npadvmod	Tulsa,Statistica
R3933	T5554	T5548	punct	", ",Statistica
R3934	T5555	T5548	npadvmod	Oklahoma,Statistica
R3935	T5556	T5548	punct	", ",Statistica
R3936	T5557	T5558	compound	United,States
R3937	T5558	T5548	npadvmod	States,Statistica
R3938	T5559	T5548	punct	),Statistica
R3939	T5560	T5515	punct	.,performed
R3940	T5562	T5563	prep	In,applied
R3941	T5564	T5562	pobj	cases,In
R3942	T5565	T5564	prep	of,cases
R3943	T5566	T5567	advmod	positively,skewed
R3944	T5567	T5568	amod	skewed,distribution
R3945	T5568	T5565	pobj	distribution,of
R3946	T5569	T5568	compound	data,distribution
R3947	T5570	T5563	punct	", ",applied
R3948	T5571	T5572	nmod	log10,x
R3949	T5572	T5574	nmod	x,transformation
R3950	T5573	T5572	punct	(,x
R3951	T5574	T5563	nsubjpass	transformation,applied
R3952	T5575	T5572	punct	+,x
R3953	T5576	T5572	nummod	0.5,x
R3954	T5577	T5572	punct	),x
R3955	T5578	T5563	auxpass	was,applied
R3956	T5579	T5563	prep	to,applied
R3957	T5580	T5581	det	the,data
R3958	T5581	T5579	pobj	data,to
R3959	T5582	T5581	amod	raw,data
R3960	T5583	T5563	prep	before,applied
R3961	T5584	T5583	pcomp	submitting,before
R3962	T5585	T5584	dobj	them,submitting
R3963	T5586	T5584	prep	to,submitting
R3964	T5587	T5586	pobj	ANOVA,to
R3965	T5588	T5563	punct	.,applied
R915	T1535	T1536	compound	Transgene,Construction
R916	T1538	T1539	nsubj	We,created
R917	T1540	T1541	det	a,vector
R918	T1541	T1539	dobj	vector,created
R919	T1542	T1543	npadvmod	tetracycline,responsive
R920	T1543	T1541	amod	responsive,vector
R921	T1544	T1543	punct	-,responsive
R922	T1545	T1541	amod	chimeric,vector
R923	T1546	T1541	nmod	mouse,vector
R924	T1547	T1546	punct	/,mouse
R925	T1548	T1546	amod	human,mouse
R926	T1549	T1550	nmod	APP695Swedish,Indiana
R927	T1550	T1541	nmod	Indiana,vector
R928	T1551	T1550	punct	/,Indiana
R929	T1552	T1550	punct	(,Indiana
R930	T1553	T1554	compound	swe,ind
R931	T1554	T1550	appos	ind,Indiana
R932	T1555	T1554	punct	/,ind
R933	T1556	T1541	punct	),vector
R934	T1557	T1539	prep	by,created
R935	T1558	T1557	pcomp	replacing,by
R936	T1559	T1560	det	the,region
R937	T1560	T1558	dobj	region,replacing
R938	T1561	T1560	compound	promoter,region
R939	T1562	T1560	prep	of,region
R940	T1563	T1564	det	the,vector
R941	T1564	T1562	pobj	vector,of
R942	T1565	T1564	compound	moPrP.XhoI,vector
R943	T1566	T1567	punct	(,known
R944	T1567	T1558	parataxis	known,replacing
R945	T1568	T1567	advmod	also,known
R946	T1569	T1567	prep	as,known
R947	T1570	T1571	nmod	pPrPpE1,3sal
R948	T1571	T1569	pobj	3sal,as
R949	T1572	T1571	punct	/,3sal
R950	T1573	T1571	nmod	E2,3sal
R951	T1574	T1571	punct	",",3sal
R952	T1575	T1576	punct	[,15
R953	T1576	T1567	parataxis	15,known
R954	T1577	T1576	punct	],15
R955	T1578	T1567	punct	),known
R956	T1579	T1558	prep	with,replacing
R957	T1580	T1581	det	the,promoter
R958	T1581	T1579	pobj	promoter,with
R959	T1582	T1583	npadvmod	tetracycline,responsive
R960	T1583	T1581	amod	responsive,promoter
R961	T1584	T1583	punct	-,responsive
R962	T1585	T1581	prep	of,promoter
R963	T1586	T1585	pobj	pTetSplice,of
R964	T1587	T1588	punct	(,Technologies
R965	T1588	T1586	parataxis	Technologies,pTetSplice
R966	T1589	T1588	compound	Life,Technologies
R967	T1590	T1588	punct	", ",Technologies
R968	T1591	T1588	npadvmod	Rockville,Technologies
R969	T1592	T1588	punct	", ",Technologies
R970	T1593	T1588	npadvmod	Maryland,Technologies
R971	T1594	T1588	punct	", ",Technologies
R972	T1595	T1596	compound	United,States
R973	T1596	T1588	npadvmod	States,Technologies
R974	T1597	T1588	punct	),Technologies
R975	T1598	T1558	punct	", ",replacing
R976	T1599	T1558	cc	and,replacing
R977	T1600	T1601	advmod	then,ligating
R978	T1601	T1558	conj	ligating,replacing
R979	T1602	T1603	compound	mouse,APP
R980	T1603	T1601	dobj	APP,ligating
R981	T1604	T1601	prep	with,ligating
R982	T1605	T1606	det	a,domain
R983	T1606	T1609	nmod	domain,cDNA
R984	T1607	T1606	amod	humanized,domain
R985	T1608	T1606	nmod	Aβ,domain
R986	T1609	T1604	pobj	cDNA,with
R987	T1610	T1606	punct	(,domain
R988	T1611	T1612	compound	mo,huAPP695
R989	T1612	T1606	appos	huAPP695,domain
R990	T1613	T1612	punct	/,huAPP695
R991	T1614	T1609	punct	),cDNA
R992	T1615	T1601	prep	into,ligating
R993	T1616	T1617	det	the,vector
R994	T1617	T1615	pobj	vector,into
R995	T1618	T1617	amod	new,vector
R996	T1619	T1539	punct	.,created
R997	T1621	T1622	nsubj	We,began
R998	T1623	T1622	prep	by,began
R999	T1624	T1623	pcomp	cloning,by

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PMC:1283364 / 5484-21072 JSON TXT 5 Projects