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PMC:1283364 / 21082-22107 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T6511 0-2 PRP denotes We
T6512 3-7 VBD denotes used
T6513 8-11 DT denotes the
T6515 12-15 NN denotes tet
T6517 15-16 HYPH denotes -
T6516 16-19 NN denotes off
T6518 20-29 NN denotes transgene
T6514 30-36 NN denotes system
T6519 37-39 TO denotes to
T6520 40-47 VB denotes express
T6521 48-49 DT denotes a
T6523 50-56 JJ denotes double
T6524 57-63 NN denotes mutant
T6522 64-71 NN denotes version
T6525 72-74 IN denotes of
T6526 75-83 JJ denotes chimeric
T6528 84-86 NN denotes mo
T6529 86-87 HYPH denotes /
T6527 87-95 NN denotes huAPP695
T6530 96-97 -LRB- denotes (
T6531 97-100 NN denotes swe
T6533 100-101 HYPH denotes /
T6534 101-104 NN denotes ind
T6532 105-110 NN denotes KM570
T6535 110-112 , denotes ,
T6536 112-117 NN denotes 571NL
T6537 117-119 , denotes ,
T6538 119-122 CC denotes and
T6539 123-128 NN denotes V617F
T6540 128-129 -RRB- denotes )
T6541 130-134 IN denotes from
T6542 135-136 DT denotes a
T6544 137-149 NN denotes tetracycline
T6546 149-150 HYPH denotes -
T6545 150-160 JJ denotes responsive
T6543 161-169 NN denotes promoter
T6547 170-171 -LRB- denotes [
T6549 171-173 CD denotes 12
T6550 173-174 , denotes ,
T6548 174-176 CD denotes 13
T6551 176-177 -RRB- denotes ]
T6552 177-178 . denotes .
T6553 178-319 sentence denotes Transgenic APP expression was activated by crossing the APPswe/ind mice to animals producing tTA under control of the CaMKIIα promoter [16].
T6554 179-189 JJ denotes Transgenic
T6555 190-193 NN denotes APP
T6556 194-204 NN denotes expression
T6558 205-208 VBD denotes was
T6557 209-218 VBN denotes activated
T6559 219-221 IN denotes by
T6560 222-230 VBG denotes crossing
T6561 231-234 DT denotes the
T6563 235-241 NN denotes APPswe
T6565 241-242 HYPH denotes /
T6564 242-245 NN denotes ind
T6562 246-250 NNS denotes mice
T6566 251-253 IN denotes to
T6567 254-261 NNS denotes animals
T6568 262-271 VBG denotes producing
T6569 272-275 NN denotes tTA
T6570 276-281 IN denotes under
T6571 282-289 NN denotes control
T6572 290-292 IN denotes of
T6573 293-296 DT denotes the
T6575 297-304 NN denotes CaMKIIα
T6574 305-313 NN denotes promoter
T6576 314-315 -LRB- denotes [
T6577 315-317 CD denotes 16
T6578 317-318 -RRB- denotes ]
T6579 318-319 . denotes .
T6580 319-513 sentence denotes After initial screening of founders, we identified four lines of tet-APPswe/ind mice that produced very high levels of transgene product in offspring coexpressing tTA (Figures 1 and Figure S1).
T6581 320-325 IN denotes After
T6583 326-333 JJ denotes initial
T6584 334-343 NN denotes screening
T6585 344-346 IN denotes of
T6586 347-355 NNS denotes founders
T6587 355-357 , denotes ,
T6588 357-359 PRP denotes we
T6582 360-370 VBD denotes identified
T6589 371-375 CD denotes four
T6590 376-381 NNS denotes lines
T6591 382-384 IN denotes of
T6592 385-388 NN denotes tet
T6594 388-389 HYPH denotes -
T6595 389-395 NN denotes APPswe
T6596 395-396 HYPH denotes /
T6593 396-399 NN denotes ind
T6597 400-404 NNS denotes mice
T6598 405-409 WDT denotes that
T6599 410-418 VBD denotes produced
T6600 419-423 RB denotes very
T6601 424-428 JJ denotes high
T6602 429-435 NNS denotes levels
T6603 436-438 IN denotes of
T6604 439-448 NN denotes transgene
T6605 449-456 NN denotes product
T6606 457-459 IN denotes in
T6607 460-469 NN denotes offspring
T6608 470-482 VBG denotes coexpressing
T6609 483-486 NN denotes tTA
T6610 487-488 -LRB- denotes (
T6611 488-495 NNS denotes Figures
T6612 496-497 CD denotes 1
T6613 498-501 CC denotes and
T6614 502-508 NN denotes Figure
T6615 509-511 NN denotes S1
T6616 511-512 -RRB- denotes )
T6617 512-513 . denotes .
T6618 513-764 sentence denotes Compared to a standard APP transgenic line used for previous amyloid studies by our laboratory (line C3–3; [15,26,27]), we estimated that the four controllable lines produce transgenic APP protein at 10- to 30-fold over endogenous levels (Figure S1).
T6619 514-522 VBN denotes Compared
T6621 523-525 IN denotes to
T6622 526-527 DT denotes a
T6624 528-536 JJ denotes standard
T6625 537-540 NN denotes APP
T6626 541-551 JJ denotes transgenic
T6623 552-556 NN denotes line
T6627 557-561 VBN denotes used
T6628 562-565 IN denotes for
T6629 566-574 JJ denotes previous
T6631 575-582 NN denotes amyloid
T6630 583-590 NNS denotes studies
T6632 591-593 IN denotes by
T6633 594-597 PRP$ denotes our
T6634 598-608 NN denotes laboratory
T6635 609-610 -LRB- denotes (
T6637 610-614 NN denotes line
T6638 615-617 NN denotes C3
T6639 617-618 HYPH denotes –
T6640 618-619 CD denotes 3
T6641 619-620 : denotes ;
T6642 621-622 -LRB- denotes [
T6643 622-624 CD denotes 15
T6644 624-625 , denotes ,
T6645 625-627 CD denotes 26
T6646 627-628 , denotes ,
T6636 628-630 CD denotes 27
T6647 630-631 -RRB- denotes ]
T6648 631-632 -RRB- denotes )
T6649 632-634 , denotes ,
T6650 634-636 PRP denotes we
T6620 637-646 VBD denotes estimated
T6651 647-651 IN denotes that
T6653 652-655 DT denotes the
T6655 656-660 CD denotes four
T6656 661-673 JJ denotes controllable
T6654 674-679 NNS denotes lines
T6652 680-687 VBP denotes produce
T6657 688-698 JJ denotes transgenic
T6659 699-702 NN denotes APP
T6658 703-710 NN denotes protein
T6660 711-713 IN denotes at
T6661 714-716 CD denotes 10
T6662 716-717 HYPH denotes -
T6663 718-720 IN denotes to
T6664 721-728 RB denotes 30-fold
T6665 729-733 IN denotes over
T6666 734-744 JJ denotes endogenous
T6667 745-751 NNS denotes levels
T6668 752-753 -LRB- denotes (
T6670 753-759 NN denotes Figure
T6669 760-762 NN denotes S1
T6671 762-763 -RRB- denotes )
T6672 763-764 . denotes .
T6673 764-1025 sentence denotes This estimate was confirmed by direct comparison of APP levels in nontransgenic and tet-off APP mice using an antibody that recognizes both endogenous APP (and amyloid precursor-like protein 2) and the transgenic protein (monoclonal antibody 22C11; Figure 1D).
T6674 765-769 DT denotes This
T6675 770-778 NN denotes estimate
T6677 779-782 VBD denotes was
T6676 783-792 VBN denotes confirmed
T6678 793-795 IN denotes by
T6679 796-802 JJ denotes direct
T6680 803-813 NN denotes comparison
T6681 814-816 IN denotes of
T6682 817-820 NN denotes APP
T6683 821-827 NNS denotes levels
T6684 828-830 IN denotes in
T6685 831-844 JJ denotes nontransgenic
T6687 845-848 CC denotes and
T6688 849-852 NN denotes tet
T6690 852-853 HYPH denotes -
T6689 853-856 NN denotes off
T6691 857-860 NN denotes APP
T6686 861-865 NNS denotes mice
T6692 866-871 VBG denotes using
T6693 872-874 DT denotes an
T6694 875-883 NN denotes antibody
T6695 884-888 WDT denotes that
T6696 889-899 VBZ denotes recognizes
T6697 900-904 CC denotes both
T6699 905-915 JJ denotes endogenous
T6698 916-919 NN denotes APP
T6700 920-921 -LRB- denotes (
T6701 921-924 CC denotes and
T6702 925-932 NN denotes amyloid
T6704 933-942 NN denotes precursor
T6706 942-943 HYPH denotes -
T6705 943-947 JJ denotes like
T6703 948-955 NN denotes protein
T6707 956-957 CD denotes 2
T6708 957-958 -RRB- denotes )
T6709 959-962 CC denotes and
T6710 963-966 DT denotes the
T6712 967-977 JJ denotes transgenic
T6711 978-985 NN denotes protein
T6713 986-987 -LRB- denotes (
T6715 987-997 JJ denotes monoclonal
T6717 998-1006 NN denotes antibody
T6716 1007-1012 NN denotes 22C11
T6718 1012-1013 : denotes ;
T6719 1014-1020 NN denotes Figure
T6714 1021-1023 NN denotes 1D
T6720 1023-1024 -RRB- denotes )
T6721 1024-1025 . denotes .
R3967 T6511 T6512 nsubj We,used
R3968 T6513 T6514 det the,system
R3969 T6514 T6512 dobj system,used
R3970 T6515 T6516 compound tet,off
R3971 T6516 T6514 compound off,system
R3972 T6517 T6516 punct -,off
R3973 T6518 T6514 compound transgene,system
R3974 T6519 T6520 aux to,express
R3975 T6520 T6512 advcl express,used
R3976 T6521 T6522 det a,version
R3977 T6522 T6520 dobj version,express
R3978 T6523 T6522 amod double,version
R3979 T6524 T6522 compound mutant,version
R3980 T6525 T6522 prep of,version
R3981 T6526 T6527 amod chimeric,huAPP695
R3982 T6527 T6525 pobj huAPP695,of
R3983 T6528 T6527 compound mo,huAPP695
R3984 T6529 T6527 punct /,huAPP695
R3985 T6530 T6522 punct (,version
R3986 T6531 T6532 compound swe,KM570
R3987 T6532 T6522 appos KM570,version
R3988 T6533 T6532 punct /,KM570
R3989 T6534 T6532 compound ind,KM570
R3990 T6535 T6532 punct ", ",KM570
R3991 T6536 T6532 conj 571NL,KM570
R3992 T6537 T6536 punct ", ",571NL
R3993 T6538 T6536 cc and,571NL
R3994 T6539 T6536 conj V617F,571NL
R3995 T6540 T6520 punct ),express
R3996 T6541 T6520 prep from,express
R3997 T6542 T6543 det a,promoter
R3998 T6543 T6541 pobj promoter,from
R3999 T6544 T6545 npadvmod tetracycline,responsive
R4000 T6545 T6543 amod responsive,promoter
R4001 T6546 T6545 punct -,responsive
R4002 T6547 T6548 punct [,13
R4003 T6548 T6520 parataxis 13,express
R4004 T6549 T6548 nummod 12,13
R4005 T6550 T6548 punct ",",13
R4006 T6551 T6548 punct ],13
R4007 T6552 T6512 punct .,used
R4008 T6554 T6555 amod Transgenic,APP
R4009 T6555 T6556 compound APP,expression
R4010 T6556 T6557 nsubjpass expression,activated
R4011 T6558 T6557 auxpass was,activated
R4012 T6559 T6557 prep by,activated
R4013 T6560 T6559 pcomp crossing,by
R4014 T6561 T6562 det the,mice
R4015 T6562 T6560 dobj mice,crossing
R4016 T6563 T6564 compound APPswe,ind
R4017 T6564 T6562 compound ind,mice
R4018 T6565 T6564 punct /,ind
R4019 T6566 T6560 prep to,crossing
R4020 T6567 T6566 pobj animals,to
R4021 T6568 T6567 acl producing,animals
R4022 T6569 T6568 dobj tTA,producing
R4023 T6570 T6568 prep under,producing
R4024 T6571 T6570 pobj control,under
R4025 T6572 T6571 prep of,control
R4026 T6573 T6574 det the,promoter
R4027 T6574 T6572 pobj promoter,of
R4028 T6575 T6574 compound CaMKIIα,promoter
R4029 T6576 T6577 punct [,16
R4030 T6577 T6568 parataxis 16,producing
R4031 T6578 T6577 punct ],16
R4032 T6579 T6557 punct .,activated
R4033 T6581 T6582 prep After,identified
R4034 T6583 T6584 amod initial,screening
R4035 T6584 T6581 pobj screening,After
R4036 T6585 T6584 prep of,screening
R4037 T6586 T6585 pobj founders,of
R4038 T6587 T6582 punct ", ",identified
R4039 T6588 T6582 nsubj we,identified
R4040 T6589 T6590 nummod four,lines
R4041 T6590 T6582 dobj lines,identified
R4042 T6591 T6590 prep of,lines
R4043 T6592 T6593 compound tet,ind
R4044 T6593 T6597 compound ind,mice
R4045 T6594 T6593 punct -,ind
R4046 T6595 T6593 compound APPswe,ind
R4047 T6596 T6593 punct /,ind
R4048 T6597 T6591 pobj mice,of
R4049 T6598 T6599 dep that,produced
R4050 T6599 T6590 relcl produced,lines
R4051 T6600 T6601 advmod very,high
R4052 T6601 T6602 amod high,levels
R4053 T6602 T6599 dobj levels,produced
R4054 T6603 T6602 prep of,levels
R4055 T6604 T6605 compound transgene,product
R4056 T6605 T6603 pobj product,of
R4057 T6606 T6599 prep in,produced
R4058 T6607 T6606 pobj offspring,in
R4059 T6608 T6607 acl coexpressing,offspring
R4060 T6609 T6608 dobj tTA,coexpressing
R4061 T6610 T6611 punct (,Figures
R4062 T6611 T6599 parataxis Figures,produced
R4063 T6612 T6611 nummod 1,Figures
R4064 T6613 T6611 cc and,Figures
R4065 T6614 T6615 compound Figure,S1
R4066 T6615 T6611 conj S1,Figures
R4067 T6616 T6611 punct ),Figures
R4068 T6617 T6582 punct .,identified
R4069 T6619 T6620 prep Compared,estimated
R4070 T6621 T6619 prep to,Compared
R4071 T6622 T6623 det a,line
R4072 T6623 T6621 pobj line,to
R4073 T6624 T6623 amod standard,line
R4074 T6625 T6623 nmod APP,line
R4075 T6626 T6623 amod transgenic,line
R4076 T6627 T6623 acl used,line
R4077 T6628 T6627 prep for,used
R4078 T6629 T6630 amod previous,studies
R4079 T6630 T6628 pobj studies,for
R4080 T6631 T6630 compound amyloid,studies
R4081 T6632 T6630 prep by,studies
R4082 T6633 T6634 poss our,laboratory
R4083 T6634 T6632 pobj laboratory,by
R4084 T6635 T6636 punct (,27
R4085 T6636 T6627 parataxis 27,used
R4086 T6637 T6638 compound line,C3
R4087 T6638 T6636 dep C3,27
R4088 T6639 T6638 punct –,C3
R4089 T6640 T6638 nummod 3,C3
R4090 T6641 T6636 punct ;,27
R4091 T6642 T6636 punct [,27
R4092 T6643 T6636 nummod 15,27
R4093 T6644 T6636 punct ",",27
R4094 T6645 T6636 nummod 26,27
R4095 T6646 T6636 punct ",",27
R4096 T6647 T6636 punct ],27
R4097 T6648 T6636 punct ),27
R4098 T6649 T6620 punct ", ",estimated
R4099 T6650 T6620 nsubj we,estimated
R4100 T6651 T6652 mark that,produce
R4101 T6652 T6620 ccomp produce,estimated
R4102 T6653 T6654 det the,lines
R4103 T6654 T6652 nsubj lines,produce
R4104 T6655 T6654 nummod four,lines
R4105 T6656 T6654 amod controllable,lines
R4106 T6657 T6658 amod transgenic,protein
R4107 T6658 T6652 dobj protein,produce
R4108 T6659 T6658 compound APP,protein
R4109 T6660 T6652 prep at,produce
R4110 T6661 T6660 pcomp 10,at
R4111 T6662 T6661 punct -,10
R4112 T6663 T6661 quantmod to,10
R4113 T6664 T6661 advmod 30-fold,10
R4114 T6665 T6661 prep over,10
R4115 T6666 T6667 amod endogenous,levels
R4116 T6667 T6665 pobj levels,over
R4117 T6668 T6669 punct (,S1
R4118 T6669 T6652 parataxis S1,produce
R4119 T6670 T6669 compound Figure,S1
R4120 T6671 T6669 punct ),S1
R4121 T6672 T6620 punct .,estimated
R4122 T6674 T6675 det This,estimate
R4123 T6675 T6676 nsubjpass estimate,confirmed
R4124 T6677 T6676 auxpass was,confirmed
R4125 T6678 T6676 agent by,confirmed
R4126 T6679 T6680 amod direct,comparison
R4127 T6680 T6678 pobj comparison,by
R4128 T6681 T6680 prep of,comparison
R4129 T6682 T6683 compound APP,levels
R4130 T6683 T6681 pobj levels,of
R4131 T6684 T6680 prep in,comparison
R4132 T6685 T6686 amod nontransgenic,mice
R4133 T6686 T6684 pobj mice,in
R4134 T6687 T6685 cc and,nontransgenic
R4135 T6688 T6689 compound tet,off
R4136 T6689 T6685 conj off,nontransgenic
R4137 T6690 T6689 punct -,off
R4138 T6691 T6686 compound APP,mice
R4139 T6692 T6676 advcl using,confirmed
R4140 T6693 T6694 det an,antibody
R4141 T6694 T6692 dobj antibody,using
R4142 T6695 T6696 dep that,recognizes
R4143 T6696 T6694 relcl recognizes,antibody
R4144 T6697 T6698 preconj both,APP
R4145 T6698 T6696 dobj APP,recognizes
R4146 T6699 T6698 amod endogenous,APP
R4147 T6700 T6698 punct (,APP
R4148 T6701 T6698 cc and,APP
R4149 T6702 T6703 nmod amyloid,protein
R4150 T6703 T6698 conj protein,APP
R4151 T6704 T6705 npadvmod precursor,like
R4152 T6705 T6703 amod like,protein
R4153 T6706 T6705 punct -,like
R4154 T6707 T6703 nummod 2,protein
R4155 T6708 T6703 punct ),protein
R4156 T6709 T6698 cc and,APP
R4157 T6710 T6711 det the,protein
R4158 T6711 T6698 conj protein,APP
R4159 T6712 T6711 amod transgenic,protein
R4160 T6713 T6714 punct (,1D
R4161 T6714 T6711 parataxis 1D,protein
R4162 T6715 T6716 amod monoclonal,22C11
R4163 T6716 T6714 dep 22C11,1D
R4164 T6717 T6716 compound antibody,22C11
R4165 T6718 T6714 punct ;,1D
R4166 T6719 T6714 compound Figure,1D
R4167 T6720 T6714 punct ),1D
R4168 T6721 T6676 punct .,confirmed