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PMC:1283364 / 17633-18756 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T4791 0-2 DT denotes An
T4792 3-10 NN denotes aliquot
T4794 11-13 IN denotes of
T4795 14-18 DT denotes each
T4797 19-27 JJ denotes cortical
T4796 28-38 NN denotes homogenate
T4798 39-43 VBN denotes used
T4799 44-47 IN denotes for
T4800 48-55 NNP denotes Western
T4801 56-64 NN denotes blotting
T4802 65-70 RB denotes above
T4803 71-74 VBD denotes was
T4804 75-84 RB denotes partially
T4793 85-96 VBN denotes solubilized
T4805 97-99 IN denotes by
T4806 100-103 DT denotes the
T4807 104-112 NN denotes addition
T4808 113-115 IN denotes of
T4809 116-119 NN denotes SDS
T4810 120-122 IN denotes to
T4811 123-124 DT denotes a
T4813 125-130 JJ denotes final
T4812 131-144 NN denotes concentration
T4814 145-147 IN denotes of
T4815 148-149 CD denotes 1
T4816 149-150 NN denotes %
T4817 150-151 . denotes .
T4818 151-339 sentence denotes Serial 1:2 dilutions were made with 1× PBS/1% SDS, and 100 μl of each dilution was then vacuum-filtered through a pre-wet 0.22-μm cellulose acetate membrane (Schleicher and Schuell) [22].
T4819 152-158 JJ denotes Serial
T4821 159-160 CD denotes 1
T4822 160-161 SYM denotes :
T4823 161-162 CD denotes 2
T4820 163-172 NNS denotes dilutions
T4825 173-177 VBD denotes were
T4824 178-182 VBN denotes made
T4826 183-187 IN denotes with
T4827 188-189 CD denotes 1
T4829 189-190 SYM denotes ×
T4828 191-194 NN denotes PBS
T4830 194-195 HYPH denotes /
T4831 195-196 CD denotes 1
T4832 196-197 NN denotes %
T4833 198-201 NN denotes SDS
T4834 201-203 , denotes ,
T4835 203-206 CC denotes and
T4836 207-210 CD denotes 100
T4837 211-213 NN denotes μl
T4839 214-216 IN denotes of
T4840 217-221 DT denotes each
T4841 222-230 NN denotes dilution
T4842 231-234 VBD denotes was
T4843 235-239 RB denotes then
T4844 240-246 NN denotes vacuum
T4845 246-247 HYPH denotes -
T4838 247-255 VBN denotes filtered
T4846 256-263 IN denotes through
T4847 264-265 DT denotes a
T4849 266-273 JJ denotes pre-wet
T4850 274-278 CD denotes 0.22
T4852 278-279 HYPH denotes -
T4851 279-281 NN denotes μm
T4853 282-291 NN denotes cellulose
T4854 292-299 NN denotes acetate
T4848 300-308 NN denotes membrane
T4855 309-310 -LRB- denotes (
T4856 310-320 NNP denotes Schleicher
T4857 321-324 CC denotes and
T4858 325-332 NNP denotes Schuell
T4859 332-333 -RRB- denotes )
T4860 334-335 -LRB- denotes [
T4861 335-337 CD denotes 22
T4862 337-338 -RRB- denotes ]
T4863 338-339 . denotes .
T4864 339-555 sentence denotes Each well was washed several times with PBS, after which blots were incubated overnight with polyclonal anti-Aβ antibody (Zymed Laboratories) diluted 1:600 in a blocking solution of 1× TBS/5% nonfat dry milk powder.
T4865 340-344 DT denotes Each
T4866 345-349 NN denotes well
T4868 350-353 VBD denotes was
T4867 354-360 VBN denotes washed
T4869 361-368 JJ denotes several
T4870 369-374 NNS denotes times
T4871 375-379 IN denotes with
T4872 380-383 NN denotes PBS
T4873 383-385 , denotes ,
T4874 385-390 IN denotes after
T4876 391-396 WDT denotes which
T4877 397-402 NNS denotes blots
T4878 403-407 VBD denotes were
T4875 408-417 VBN denotes incubated
T4879 418-427 RB denotes overnight
T4880 428-432 IN denotes with
T4881 433-443 JJ denotes polyclonal
T4883 444-451 JJ denotes anti-Aβ
T4882 452-460 NN denotes antibody
T4884 461-462 -LRB- denotes (
T4886 462-467 NNP denotes Zymed
T4885 468-480 NNP denotes Laboratories
T4887 480-481 -RRB- denotes )
T4888 482-489 VBN denotes diluted
T4889 490-491 CD denotes 1
T4890 491-492 SYM denotes :
T4891 492-495 CD denotes 600
T4892 496-498 IN denotes in
T4893 499-500 DT denotes a
T4895 501-509 NN denotes blocking
T4894 510-518 NN denotes solution
T4896 519-521 IN denotes of
T4897 522-523 CD denotes 1
T4899 523-524 SYM denotes ×
T4898 525-528 NN denotes TBS
T4901 528-529 HYPH denotes /
T4902 529-530 CD denotes 5
T4903 530-531 NN denotes %
T4905 532-538 JJ denotes nonfat
T4906 539-542 JJ denotes dry
T4904 543-547 NN denotes milk
T4900 548-554 NN denotes powder
T4907 554-555 . denotes .
T4908 555-743 sentence denotes After washing the blots three times for 10 min each in 1× TBS/0.1% Tween-20, the membrane was incubated for 1 h with HRP-conjugated protein A (Sigma) diluted 1:5,000 in blocking solution.
T4909 556-561 IN denotes After
T4911 562-569 VBG denotes washing
T4912 570-573 DT denotes the
T4913 574-579 NNS denotes blots
T4914 580-585 CD denotes three
T4915 586-591 NNS denotes times
T4916 592-595 IN denotes for
T4917 596-598 CD denotes 10
T4918 599-602 NN denotes min
T4919 603-607 DT denotes each
T4920 608-610 IN denotes in
T4921 611-612 CD denotes 1
T4923 612-613 SYM denotes ×
T4922 614-617 NN denotes TBS
T4924 617-618 HYPH denotes /
T4925 618-621 CD denotes 0.1
T4926 621-622 NN denotes %
T4927 623-628 NN denotes Tween
T4928 628-629 HYPH denotes -
T4929 629-631 CD denotes 20
T4930 631-633 , denotes ,
T4931 633-636 DT denotes the
T4932 637-645 NN denotes membrane
T4933 646-649 VBD denotes was
T4910 650-659 VBN denotes incubated
T4934 660-663 IN denotes for
T4935 664-665 CD denotes 1
T4936 666-667 NN denotes h
T4937 668-672 IN denotes with
T4938 673-676 NN denotes HRP
T4940 676-677 HYPH denotes -
T4939 677-687 VBN denotes conjugated
T4942 688-695 NN denotes protein
T4941 696-697 NN denotes A
T4943 698-699 -LRB- denotes (
T4944 699-704 NNP denotes Sigma
T4945 704-705 -RRB- denotes )
T4946 706-713 VBN denotes diluted
T4947 714-715 CD denotes 1
T4948 715-716 SYM denotes :
T4949 716-721 CD denotes 5,000
T4950 722-724 IN denotes in
T4951 725-733 NN denotes blocking
T4952 734-742 NN denotes solution
T4953 742-743 . denotes .
T4954 743-936 sentence denotes The membranes were again washed three times with 1× TBS/0.1% Tween-20, before antibody binding was detected with enhanced chemiluminescence (PerkinElmer, Boston, Massachusetts, United States).
T4955 744-747 DT denotes The
T4956 748-757 NNS denotes membranes
T4958 758-762 VBD denotes were
T4959 763-768 RB denotes again
T4957 769-775 VBN denotes washed
T4960 776-781 CD denotes three
T4961 782-787 NNS denotes times
T4962 788-792 IN denotes with
T4963 793-794 CD denotes 1
T4965 794-795 SYM denotes ×
T4964 796-799 NN denotes TBS
T4966 799-800 HYPH denotes /
T4967 800-803 CD denotes 0.1
T4968 803-804 NN denotes %
T4969 805-810 NN denotes Tween
T4970 810-811 HYPH denotes -
T4971 811-813 CD denotes 20
T4972 813-815 , denotes ,
T4973 815-821 IN denotes before
T4975 822-830 NN denotes antibody
T4976 831-838 NN denotes binding
T4977 839-842 VBD denotes was
T4974 843-851 VBN denotes detected
T4978 852-856 IN denotes with
T4979 857-865 VBN denotes enhanced
T4980 866-883 NN denotes chemiluminescence
T4981 884-885 -LRB- denotes (
T4982 885-896 NNP denotes PerkinElmer
T4983 896-898 , denotes ,
T4984 898-904 NNP denotes Boston
T4985 904-906 , denotes ,
T4986 906-919 NNP denotes Massachusetts
T4987 919-921 , denotes ,
T4988 921-927 NNP denotes United
T4989 928-934 NNP denotes States
T4990 934-935 -RRB- denotes )
T4991 935-936 . denotes .
T4992 936-1123 sentence denotes Digital images of each blot were captured with a Molecular Imager FX gel documentation system, and the intensity of Aβ staining was quantified using Quantity One image analysis software.
T4993 937-944 JJ denotes Digital
T4994 945-951 NNS denotes images
T4996 952-954 IN denotes of
T4997 955-959 DT denotes each
T4998 960-964 NN denotes blot
T4999 965-969 VBD denotes were
T4995 970-978 VBN denotes captured
T5000 979-983 IN denotes with
T5001 984-985 DT denotes a
T5003 986-995 JJ denotes Molecular
T5004 996-1002 NNP denotes Imager
T5005 1003-1005 NNP denotes FX
T5006 1006-1009 NN denotes gel
T5007 1010-1023 NN denotes documentation
T5002 1024-1030 NN denotes system
T5008 1030-1032 , denotes ,
T5009 1032-1035 CC denotes and
T5010 1036-1039 DT denotes the
T5011 1040-1049 NN denotes intensity
T5013 1050-1052 IN denotes of
T5014 1053-1055 NN denotes Aβ
T5015 1056-1064 NN denotes staining
T5016 1065-1068 VBD denotes was
T5012 1069-1079 VBN denotes quantified
T5017 1080-1085 VBG denotes using
T5018 1086-1094 NNP denotes Quantity
T5020 1095-1098 CD denotes One
T5021 1099-1104 NN denotes image
T5022 1105-1113 NN denotes analysis
T5019 1114-1122 NN denotes software
T5023 1122-1123 . denotes .
R3285 T4791 T4792 det An,aliquot
R3286 T4792 T4793 nsubjpass aliquot,solubilized
R3287 T4794 T4792 prep of,aliquot
R3288 T4795 T4796 det each,homogenate
R3289 T4796 T4794 pobj homogenate,of
R3290 T4797 T4796 amod cortical,homogenate
R3291 T4798 T4796 acl used,homogenate
R3292 T4799 T4798 prep for,used
R3293 T4800 T4801 compound Western,blotting
R3294 T4801 T4799 pobj blotting,for
R3295 T4802 T4798 advmod above,used
R3296 T4803 T4793 auxpass was,solubilized
R3297 T4804 T4793 advmod partially,solubilized
R3298 T4805 T4793 prep by,solubilized
R3299 T4806 T4807 det the,addition
R3300 T4807 T4805 pobj addition,by
R3301 T4808 T4807 prep of,addition
R3302 T4809 T4808 pobj SDS,of
R3303 T4810 T4793 prep to,solubilized
R3304 T4811 T4812 det a,concentration
R3305 T4812 T4810 pobj concentration,to
R3306 T4813 T4812 amod final,concentration
R3307 T4814 T4812 prep of,concentration
R3308 T4815 T4816 nummod 1,%
R3309 T4816 T4814 pobj %,of
R3310 T4817 T4793 punct .,solubilized
R3311 T4819 T4820 amod Serial,dilutions
R3312 T4820 T4824 nsubjpass dilutions,made
R3313 T4821 T4820 nummod 1,dilutions
R3314 T4822 T4823 punct :,2
R3315 T4823 T4821 prep 2,1
R3316 T4825 T4824 auxpass were,made
R3317 T4826 T4824 prep with,made
R3318 T4827 T4828 nummod 1,PBS
R3319 T4828 T4826 pobj PBS,with
R3320 T4829 T4827 punct ×,1
R3321 T4830 T4828 punct /,PBS
R3322 T4831 T4832 nummod 1,%
R3323 T4832 T4833 compound %,SDS
R3324 T4833 T4828 appos SDS,PBS
R3325 T4834 T4824 punct ", ",made
R3326 T4835 T4824 cc and,made
R3327 T4836 T4837 nummod 100,μl
R3328 T4837 T4838 nsubjpass μl,filtered
R3329 T4838 T4824 conj filtered,made
R3330 T4839 T4837 prep of,μl
R3331 T4840 T4841 det each,dilution
R3332 T4841 T4839 pobj dilution,of
R3333 T4842 T4838 auxpass was,filtered
R3334 T4843 T4838 advmod then,filtered
R3335 T4844 T4838 dep vacuum,filtered
R3336 T4845 T4838 punct -,filtered
R3337 T4846 T4838 prep through,filtered
R3338 T4847 T4848 det a,membrane
R3339 T4848 T4846 pobj membrane,through
R3340 T4849 T4848 amod pre-wet,membrane
R3341 T4850 T4851 nummod 0.22,μm
R3342 T4851 T4848 compound μm,membrane
R3343 T4852 T4851 punct -,μm
R3344 T4853 T4848 compound cellulose,membrane
R3345 T4854 T4848 compound acetate,membrane
R3346 T4855 T4856 punct (,Schleicher
R3347 T4856 T4848 parataxis Schleicher,membrane
R3348 T4857 T4856 cc and,Schleicher
R3349 T4858 T4856 conj Schuell,Schleicher
R3350 T4859 T4856 punct ),Schleicher
R3351 T4860 T4861 punct [,22
R3352 T4861 T4838 parataxis 22,filtered
R3353 T4862 T4861 punct ],22
R3354 T4863 T4838 punct .,filtered
R3355 T4865 T4866 det Each,well
R3356 T4866 T4867 nsubjpass well,washed
R3357 T4868 T4867 auxpass was,washed
R3358 T4869 T4870 amod several,times
R3359 T4870 T4867 dobj times,washed
R3360 T4871 T4867 prep with,washed
R3361 T4872 T4871 pobj PBS,with
R3362 T4873 T4867 punct ", ",washed
R3363 T4874 T4875 prep after,incubated
R3364 T4875 T4867 advcl incubated,washed
R3365 T4876 T4874 pobj which,after
R3366 T4877 T4875 nsubjpass blots,incubated
R3367 T4878 T4875 auxpass were,incubated
R3368 T4879 T4875 advmod overnight,incubated
R3369 T4880 T4875 prep with,incubated
R3370 T4881 T4882 amod polyclonal,antibody
R3371 T4882 T4880 pobj antibody,with
R3372 T4883 T4882 amod anti-Aβ,antibody
R3373 T4884 T4885 punct (,Laboratories
R3374 T4885 T4882 parataxis Laboratories,antibody
R3375 T4886 T4885 compound Zymed,Laboratories
R3376 T4887 T4885 punct ),Laboratories
R3377 T4888 T4882 acl diluted,antibody
R3378 T4889 T4888 npadvmod 1,diluted
R3379 T4890 T4891 punct :,600
R3380 T4891 T4889 prep 600,1
R3381 T4892 T4888 prep in,diluted
R3382 T4893 T4894 det a,solution
R3383 T4894 T4892 pobj solution,in
R3384 T4895 T4894 compound blocking,solution
R3385 T4896 T4894 prep of,solution
R3386 T4897 T4898 nummod 1,TBS
R3387 T4898 T4900 nmod TBS,powder
R3388 T4899 T4897 punct ×,1
R3389 T4900 T4896 pobj powder,of
R3390 T4901 T4898 punct /,TBS
R3391 T4902 T4903 nummod 5,%
R3392 T4903 T4904 nmod %,milk
R3393 T4904 T4898 appos milk,TBS
R3394 T4905 T4904 amod nonfat,milk
R3395 T4906 T4904 amod dry,milk
R3396 T4907 T4867 punct .,washed
R3397 T4909 T4910 prep After,incubated
R3398 T4911 T4909 pcomp washing,After
R3399 T4912 T4913 det the,blots
R3400 T4913 T4911 dobj blots,washing
R3401 T4914 T4915 nummod three,times
R3402 T4915 T4911 npadvmod times,washing
R3403 T4916 T4911 prep for,washing
R3404 T4917 T4918 nummod 10,min
R3405 T4918 T4916 pobj min,for
R3406 T4919 T4918 appos each,min
R3407 T4920 T4911 prep in,washing
R3408 T4921 T4922 nummod 1,TBS
R3409 T4922 T4920 pobj TBS,in
R3410 T4923 T4921 punct ×,1
R3411 T4924 T4922 punct /,TBS
R3412 T4925 T4926 nummod 0.1,%
R3413 T4926 T4927 compound %,Tween
R3414 T4927 T4922 appos Tween,TBS
R3415 T4928 T4927 punct -,Tween
R3416 T4929 T4927 nummod 20,Tween
R3417 T4930 T4910 punct ", ",incubated
R3418 T4931 T4932 det the,membrane
R3419 T4932 T4910 nsubjpass membrane,incubated
R3420 T4933 T4910 auxpass was,incubated
R3421 T4934 T4910 prep for,incubated
R3422 T4935 T4936 nummod 1,h
R3423 T4936 T4934 pobj h,for
R3424 T4937 T4910 prep with,incubated
R3425 T4938 T4939 npadvmod HRP,conjugated
R3426 T4939 T4941 amod conjugated,A
R3427 T4940 T4939 punct -,conjugated
R3428 T4941 T4937 pobj A,with
R3429 T4942 T4941 compound protein,A
R3430 T4943 T4944 punct (,Sigma
R3431 T4944 T4941 parataxis Sigma,A
R3432 T4945 T4944 punct ),Sigma
R3433 T4946 T4941 acl diluted,A
R3434 T4947 T4946 dobj 1,diluted
R3435 T4948 T4949 punct :,"5,000"
R3436 T4949 T4947 prep "5,000",1
R3437 T4950 T4946 prep in,diluted
R3438 T4951 T4952 compound blocking,solution
R3439 T4952 T4950 pobj solution,in
R3440 T4953 T4910 punct .,incubated
R3441 T4955 T4956 det The,membranes
R3442 T4956 T4957 nsubjpass membranes,washed
R3443 T4958 T4957 auxpass were,washed
R3444 T4959 T4957 advmod again,washed
R3445 T4960 T4961 nummod three,times
R3446 T4961 T4957 npadvmod times,washed
R3447 T4962 T4957 prep with,washed
R3448 T4963 T4964 nummod 1,TBS
R3449 T4964 T4962 pobj TBS,with
R3450 T4965 T4963 punct ×,1
R3451 T4966 T4964 punct /,TBS
R3452 T4967 T4968 nummod 0.1,%
R3453 T4968 T4969 compound %,Tween
R3454 T4969 T4964 appos Tween,TBS
R3455 T4970 T4969 punct -,Tween
R3456 T4971 T4969 nummod 20,Tween
R3457 T4972 T4957 punct ", ",washed
R3458 T4973 T4974 mark before,detected
R3459 T4974 T4957 advcl detected,washed
R3460 T4975 T4976 compound antibody,binding
R3461 T4976 T4974 nsubjpass binding,detected
R3462 T4977 T4974 auxpass was,detected
R3463 T4978 T4974 prep with,detected
R3464 T4979 T4980 amod enhanced,chemiluminescence
R3465 T4980 T4978 pobj chemiluminescence,with
R3466 T4981 T4982 punct (,PerkinElmer
R3467 T4982 T4974 parataxis PerkinElmer,detected
R3468 T4983 T4982 punct ", ",PerkinElmer
R3469 T4984 T4982 npadvmod Boston,PerkinElmer
R3470 T4985 T4982 punct ", ",PerkinElmer
R3471 T4986 T4982 npadvmod Massachusetts,PerkinElmer
R3472 T4987 T4982 punct ", ",PerkinElmer
R3473 T4988 T4989 compound United,States
R3474 T4989 T4982 npadvmod States,PerkinElmer
R3475 T4990 T4982 punct ),PerkinElmer
R3476 T4991 T4957 punct .,washed
R3477 T4993 T4994 amod Digital,images
R3478 T4994 T4995 nsubjpass images,captured
R3479 T4996 T4994 prep of,images
R3480 T4997 T4998 det each,blot
R3481 T4998 T4996 pobj blot,of
R3482 T4999 T4995 auxpass were,captured
R3483 T5000 T4995 prep with,captured
R3484 T5001 T5002 det a,system
R3485 T5002 T5000 pobj system,with
R3486 T5003 T5004 amod Molecular,Imager
R3487 T5004 T5005 compound Imager,FX
R3488 T5005 T5002 compound FX,system
R3489 T5006 T5007 compound gel,documentation
R3490 T5007 T5002 compound documentation,system
R3491 T5008 T4995 punct ", ",captured
R3492 T5009 T4995 cc and,captured
R3493 T5010 T5011 det the,intensity
R3494 T5011 T5012 nsubjpass intensity,quantified
R3495 T5012 T4995 conj quantified,captured
R3496 T5013 T5011 prep of,intensity
R3497 T5014 T5015 compound Aβ,staining
R3498 T5015 T5013 pobj staining,of
R3499 T5016 T5012 auxpass was,quantified
R3500 T5017 T5012 advcl using,quantified
R3501 T5018 T5019 nmod Quantity,software
R3502 T5019 T5017 dobj software,using
R3503 T5020 T5018 nummod One,Quantity
R3504 T5021 T5022 compound image,analysis
R3505 T5022 T5019 compound analysis,software
R3506 T5023 T5012 punct .,quantified