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PMC:1283364 / 17615-18756 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T4787 0-6 NN denotes Filter
T4789 7-11 NN denotes Trap
T4788 12-17 NN denotes Assay
T4790 17-169 sentence denotes An aliquot of each cortical homogenate used for Western blotting above was partially solubilized by the addition of SDS to a final concentration of 1%.
T4791 18-20 DT denotes An
T4792 21-28 NN denotes aliquot
T4794 29-31 IN denotes of
T4795 32-36 DT denotes each
T4797 37-45 JJ denotes cortical
T4796 46-56 NN denotes homogenate
T4798 57-61 VBN denotes used
T4799 62-65 IN denotes for
T4800 66-73 NNP denotes Western
T4801 74-82 NN denotes blotting
T4802 83-88 RB denotes above
T4803 89-92 VBD denotes was
T4804 93-102 RB denotes partially
T4793 103-114 VBN denotes solubilized
T4805 115-117 IN denotes by
T4806 118-121 DT denotes the
T4807 122-130 NN denotes addition
T4808 131-133 IN denotes of
T4809 134-137 NN denotes SDS
T4810 138-140 IN denotes to
T4811 141-142 DT denotes a
T4813 143-148 JJ denotes final
T4812 149-162 NN denotes concentration
T4814 163-165 IN denotes of
T4815 166-167 CD denotes 1
T4816 167-168 NN denotes %
T4817 168-169 . denotes .
T4818 169-357 sentence denotes Serial 1:2 dilutions were made with 1× PBS/1% SDS, and 100 μl of each dilution was then vacuum-filtered through a pre-wet 0.22-μm cellulose acetate membrane (Schleicher and Schuell) [22].
T4819 170-176 JJ denotes Serial
T4821 177-178 CD denotes 1
T4822 178-179 SYM denotes :
T4823 179-180 CD denotes 2
T4820 181-190 NNS denotes dilutions
T4825 191-195 VBD denotes were
T4824 196-200 VBN denotes made
T4826 201-205 IN denotes with
T4827 206-207 CD denotes 1
T4829 207-208 SYM denotes ×
T4828 209-212 NN denotes PBS
T4830 212-213 HYPH denotes /
T4831 213-214 CD denotes 1
T4832 214-215 NN denotes %
T4833 216-219 NN denotes SDS
T4834 219-221 , denotes ,
T4835 221-224 CC denotes and
T4836 225-228 CD denotes 100
T4837 229-231 NN denotes μl
T4839 232-234 IN denotes of
T4840 235-239 DT denotes each
T4841 240-248 NN denotes dilution
T4842 249-252 VBD denotes was
T4843 253-257 RB denotes then
T4844 258-264 NN denotes vacuum
T4845 264-265 HYPH denotes -
T4838 265-273 VBN denotes filtered
T4846 274-281 IN denotes through
T4847 282-283 DT denotes a
T4849 284-291 JJ denotes pre-wet
T4850 292-296 CD denotes 0.22
T4852 296-297 HYPH denotes -
T4851 297-299 NN denotes μm
T4853 300-309 NN denotes cellulose
T4854 310-317 NN denotes acetate
T4848 318-326 NN denotes membrane
T4855 327-328 -LRB- denotes (
T4856 328-338 NNP denotes Schleicher
T4857 339-342 CC denotes and
T4858 343-350 NNP denotes Schuell
T4859 350-351 -RRB- denotes )
T4860 352-353 -LRB- denotes [
T4861 353-355 CD denotes 22
T4862 355-356 -RRB- denotes ]
T4863 356-357 . denotes .
T4864 357-573 sentence denotes Each well was washed several times with PBS, after which blots were incubated overnight with polyclonal anti-Aβ antibody (Zymed Laboratories) diluted 1:600 in a blocking solution of 1× TBS/5% nonfat dry milk powder.
T4865 358-362 DT denotes Each
T4866 363-367 NN denotes well
T4868 368-371 VBD denotes was
T4867 372-378 VBN denotes washed
T4869 379-386 JJ denotes several
T4870 387-392 NNS denotes times
T4871 393-397 IN denotes with
T4872 398-401 NN denotes PBS
T4873 401-403 , denotes ,
T4874 403-408 IN denotes after
T4876 409-414 WDT denotes which
T4877 415-420 NNS denotes blots
T4878 421-425 VBD denotes were
T4875 426-435 VBN denotes incubated
T4879 436-445 RB denotes overnight
T4880 446-450 IN denotes with
T4881 451-461 JJ denotes polyclonal
T4883 462-469 JJ denotes anti-Aβ
T4882 470-478 NN denotes antibody
T4884 479-480 -LRB- denotes (
T4886 480-485 NNP denotes Zymed
T4885 486-498 NNP denotes Laboratories
T4887 498-499 -RRB- denotes )
T4888 500-507 VBN denotes diluted
T4889 508-509 CD denotes 1
T4890 509-510 SYM denotes :
T4891 510-513 CD denotes 600
T4892 514-516 IN denotes in
T4893 517-518 DT denotes a
T4895 519-527 NN denotes blocking
T4894 528-536 NN denotes solution
T4896 537-539 IN denotes of
T4897 540-541 CD denotes 1
T4899 541-542 SYM denotes ×
T4898 543-546 NN denotes TBS
T4901 546-547 HYPH denotes /
T4902 547-548 CD denotes 5
T4903 548-549 NN denotes %
T4905 550-556 JJ denotes nonfat
T4906 557-560 JJ denotes dry
T4904 561-565 NN denotes milk
T4900 566-572 NN denotes powder
T4907 572-573 . denotes .
T4908 573-761 sentence denotes After washing the blots three times for 10 min each in 1× TBS/0.1% Tween-20, the membrane was incubated for 1 h with HRP-conjugated protein A (Sigma) diluted 1:5,000 in blocking solution.
T4909 574-579 IN denotes After
T4911 580-587 VBG denotes washing
T4912 588-591 DT denotes the
T4913 592-597 NNS denotes blots
T4914 598-603 CD denotes three
T4915 604-609 NNS denotes times
T4916 610-613 IN denotes for
T4917 614-616 CD denotes 10
T4918 617-620 NN denotes min
T4919 621-625 DT denotes each
T4920 626-628 IN denotes in
T4921 629-630 CD denotes 1
T4923 630-631 SYM denotes ×
T4922 632-635 NN denotes TBS
T4924 635-636 HYPH denotes /
T4925 636-639 CD denotes 0.1
T4926 639-640 NN denotes %
T4927 641-646 NN denotes Tween
T4928 646-647 HYPH denotes -
T4929 647-649 CD denotes 20
T4930 649-651 , denotes ,
T4931 651-654 DT denotes the
T4932 655-663 NN denotes membrane
T4933 664-667 VBD denotes was
T4910 668-677 VBN denotes incubated
T4934 678-681 IN denotes for
T4935 682-683 CD denotes 1
T4936 684-685 NN denotes h
T4937 686-690 IN denotes with
T4938 691-694 NN denotes HRP
T4940 694-695 HYPH denotes -
T4939 695-705 VBN denotes conjugated
T4942 706-713 NN denotes protein
T4941 714-715 NN denotes A
T4943 716-717 -LRB- denotes (
T4944 717-722 NNP denotes Sigma
T4945 722-723 -RRB- denotes )
T4946 724-731 VBN denotes diluted
T4947 732-733 CD denotes 1
T4948 733-734 SYM denotes :
T4949 734-739 CD denotes 5,000
T4950 740-742 IN denotes in
T4951 743-751 NN denotes blocking
T4952 752-760 NN denotes solution
T4953 760-761 . denotes .
T4954 761-954 sentence denotes The membranes were again washed three times with 1× TBS/0.1% Tween-20, before antibody binding was detected with enhanced chemiluminescence (PerkinElmer, Boston, Massachusetts, United States).
T4955 762-765 DT denotes The
T4956 766-775 NNS denotes membranes
T4958 776-780 VBD denotes were
T4959 781-786 RB denotes again
T4957 787-793 VBN denotes washed
T4960 794-799 CD denotes three
T4961 800-805 NNS denotes times
T4962 806-810 IN denotes with
T4963 811-812 CD denotes 1
T4965 812-813 SYM denotes ×
T4964 814-817 NN denotes TBS
T4966 817-818 HYPH denotes /
T4967 818-821 CD denotes 0.1
T4968 821-822 NN denotes %
T4969 823-828 NN denotes Tween
T4970 828-829 HYPH denotes -
T4971 829-831 CD denotes 20
T4972 831-833 , denotes ,
T4973 833-839 IN denotes before
T4975 840-848 NN denotes antibody
T4976 849-856 NN denotes binding
T4977 857-860 VBD denotes was
T4974 861-869 VBN denotes detected
T4978 870-874 IN denotes with
T4979 875-883 VBN denotes enhanced
T4980 884-901 NN denotes chemiluminescence
T4981 902-903 -LRB- denotes (
T4982 903-914 NNP denotes PerkinElmer
T4983 914-916 , denotes ,
T4984 916-922 NNP denotes Boston
T4985 922-924 , denotes ,
T4986 924-937 NNP denotes Massachusetts
T4987 937-939 , denotes ,
T4988 939-945 NNP denotes United
T4989 946-952 NNP denotes States
T4990 952-953 -RRB- denotes )
T4991 953-954 . denotes .
T4992 954-1141 sentence denotes Digital images of each blot were captured with a Molecular Imager FX gel documentation system, and the intensity of Aβ staining was quantified using Quantity One image analysis software.
T4993 955-962 JJ denotes Digital
T4994 963-969 NNS denotes images
T4996 970-972 IN denotes of
T4997 973-977 DT denotes each
T4998 978-982 NN denotes blot
T4999 983-987 VBD denotes were
T4995 988-996 VBN denotes captured
T5000 997-1001 IN denotes with
T5001 1002-1003 DT denotes a
T5003 1004-1013 JJ denotes Molecular
T5004 1014-1020 NNP denotes Imager
T5005 1021-1023 NNP denotes FX
T5006 1024-1027 NN denotes gel
T5007 1028-1041 NN denotes documentation
T5002 1042-1048 NN denotes system
T5008 1048-1050 , denotes ,
T5009 1050-1053 CC denotes and
T5010 1054-1057 DT denotes the
T5011 1058-1067 NN denotes intensity
T5013 1068-1070 IN denotes of
T5014 1071-1073 NN denotes Aβ
T5015 1074-1082 NN denotes staining
T5016 1083-1086 VBD denotes was
T5012 1087-1097 VBN denotes quantified
T5017 1098-1103 VBG denotes using
T5018 1104-1112 NNP denotes Quantity
T5020 1113-1116 CD denotes One
T5021 1117-1122 NN denotes image
T5022 1123-1131 NN denotes analysis
T5019 1132-1140 NN denotes software
T5023 1140-1141 . denotes .
R3283 T4787 T4788 compound Filter,Assay
R3284 T4789 T4788 compound Trap,Assay
R3285 T4791 T4792 det An,aliquot
R3286 T4792 T4793 nsubjpass aliquot,solubilized
R3287 T4794 T4792 prep of,aliquot
R3288 T4795 T4796 det each,homogenate
R3289 T4796 T4794 pobj homogenate,of
R3290 T4797 T4796 amod cortical,homogenate
R3291 T4798 T4796 acl used,homogenate
R3292 T4799 T4798 prep for,used
R3293 T4800 T4801 compound Western,blotting
R3294 T4801 T4799 pobj blotting,for
R3295 T4802 T4798 advmod above,used
R3296 T4803 T4793 auxpass was,solubilized
R3297 T4804 T4793 advmod partially,solubilized
R3298 T4805 T4793 prep by,solubilized
R3299 T4806 T4807 det the,addition
R3300 T4807 T4805 pobj addition,by
R3301 T4808 T4807 prep of,addition
R3302 T4809 T4808 pobj SDS,of
R3303 T4810 T4793 prep to,solubilized
R3304 T4811 T4812 det a,concentration
R3305 T4812 T4810 pobj concentration,to
R3306 T4813 T4812 amod final,concentration
R3307 T4814 T4812 prep of,concentration
R3308 T4815 T4816 nummod 1,%
R3309 T4816 T4814 pobj %,of
R3310 T4817 T4793 punct .,solubilized
R3311 T4819 T4820 amod Serial,dilutions
R3312 T4820 T4824 nsubjpass dilutions,made
R3313 T4821 T4820 nummod 1,dilutions
R3314 T4822 T4823 punct :,2
R3315 T4823 T4821 prep 2,1
R3316 T4825 T4824 auxpass were,made
R3317 T4826 T4824 prep with,made
R3318 T4827 T4828 nummod 1,PBS
R3319 T4828 T4826 pobj PBS,with
R3320 T4829 T4827 punct ×,1
R3321 T4830 T4828 punct /,PBS
R3322 T4831 T4832 nummod 1,%
R3323 T4832 T4833 compound %,SDS
R3324 T4833 T4828 appos SDS,PBS
R3325 T4834 T4824 punct ", ",made
R3326 T4835 T4824 cc and,made
R3327 T4836 T4837 nummod 100,μl
R3328 T4837 T4838 nsubjpass μl,filtered
R3329 T4838 T4824 conj filtered,made
R3330 T4839 T4837 prep of,μl
R3331 T4840 T4841 det each,dilution
R3332 T4841 T4839 pobj dilution,of
R3333 T4842 T4838 auxpass was,filtered
R3334 T4843 T4838 advmod then,filtered
R3335 T4844 T4838 dep vacuum,filtered
R3336 T4845 T4838 punct -,filtered
R3337 T4846 T4838 prep through,filtered
R3338 T4847 T4848 det a,membrane
R3339 T4848 T4846 pobj membrane,through
R3340 T4849 T4848 amod pre-wet,membrane
R3341 T4850 T4851 nummod 0.22,μm
R3342 T4851 T4848 compound μm,membrane
R3343 T4852 T4851 punct -,μm
R3344 T4853 T4848 compound cellulose,membrane
R3345 T4854 T4848 compound acetate,membrane
R3346 T4855 T4856 punct (,Schleicher
R3347 T4856 T4848 parataxis Schleicher,membrane
R3348 T4857 T4856 cc and,Schleicher
R3349 T4858 T4856 conj Schuell,Schleicher
R3350 T4859 T4856 punct ),Schleicher
R3351 T4860 T4861 punct [,22
R3352 T4861 T4838 parataxis 22,filtered
R3353 T4862 T4861 punct ],22
R3354 T4863 T4838 punct .,filtered
R3355 T4865 T4866 det Each,well
R3356 T4866 T4867 nsubjpass well,washed
R3357 T4868 T4867 auxpass was,washed
R3358 T4869 T4870 amod several,times
R3359 T4870 T4867 dobj times,washed
R3360 T4871 T4867 prep with,washed
R3361 T4872 T4871 pobj PBS,with
R3362 T4873 T4867 punct ", ",washed
R3363 T4874 T4875 prep after,incubated
R3364 T4875 T4867 advcl incubated,washed
R3365 T4876 T4874 pobj which,after
R3366 T4877 T4875 nsubjpass blots,incubated
R3367 T4878 T4875 auxpass were,incubated
R3368 T4879 T4875 advmod overnight,incubated
R3369 T4880 T4875 prep with,incubated
R3370 T4881 T4882 amod polyclonal,antibody
R3371 T4882 T4880 pobj antibody,with
R3372 T4883 T4882 amod anti-Aβ,antibody
R3373 T4884 T4885 punct (,Laboratories
R3374 T4885 T4882 parataxis Laboratories,antibody
R3375 T4886 T4885 compound Zymed,Laboratories
R3376 T4887 T4885 punct ),Laboratories
R3377 T4888 T4882 acl diluted,antibody
R3378 T4889 T4888 npadvmod 1,diluted
R3379 T4890 T4891 punct :,600
R3380 T4891 T4889 prep 600,1
R3381 T4892 T4888 prep in,diluted
R3382 T4893 T4894 det a,solution
R3383 T4894 T4892 pobj solution,in
R3384 T4895 T4894 compound blocking,solution
R3385 T4896 T4894 prep of,solution
R3386 T4897 T4898 nummod 1,TBS
R3387 T4898 T4900 nmod TBS,powder
R3388 T4899 T4897 punct ×,1
R3389 T4900 T4896 pobj powder,of
R3390 T4901 T4898 punct /,TBS
R3391 T4902 T4903 nummod 5,%
R3392 T4903 T4904 nmod %,milk
R3393 T4904 T4898 appos milk,TBS
R3394 T4905 T4904 amod nonfat,milk
R3395 T4906 T4904 amod dry,milk
R3396 T4907 T4867 punct .,washed
R3397 T4909 T4910 prep After,incubated
R3398 T4911 T4909 pcomp washing,After
R3399 T4912 T4913 det the,blots
R3400 T4913 T4911 dobj blots,washing
R3401 T4914 T4915 nummod three,times
R3402 T4915 T4911 npadvmod times,washing
R3403 T4916 T4911 prep for,washing
R3404 T4917 T4918 nummod 10,min
R3405 T4918 T4916 pobj min,for
R3406 T4919 T4918 appos each,min
R3407 T4920 T4911 prep in,washing
R3408 T4921 T4922 nummod 1,TBS
R3409 T4922 T4920 pobj TBS,in
R3410 T4923 T4921 punct ×,1
R3411 T4924 T4922 punct /,TBS
R3412 T4925 T4926 nummod 0.1,%
R3413 T4926 T4927 compound %,Tween
R3414 T4927 T4922 appos Tween,TBS
R3415 T4928 T4927 punct -,Tween
R3416 T4929 T4927 nummod 20,Tween
R3417 T4930 T4910 punct ", ",incubated
R3418 T4931 T4932 det the,membrane
R3419 T4932 T4910 nsubjpass membrane,incubated
R3420 T4933 T4910 auxpass was,incubated
R3421 T4934 T4910 prep for,incubated
R3422 T4935 T4936 nummod 1,h
R3423 T4936 T4934 pobj h,for
R3424 T4937 T4910 prep with,incubated
R3425 T4938 T4939 npadvmod HRP,conjugated
R3426 T4939 T4941 amod conjugated,A
R3427 T4940 T4939 punct -,conjugated
R3428 T4941 T4937 pobj A,with
R3429 T4942 T4941 compound protein,A
R3430 T4943 T4944 punct (,Sigma
R3431 T4944 T4941 parataxis Sigma,A
R3432 T4945 T4944 punct ),Sigma
R3433 T4946 T4941 acl diluted,A
R3434 T4947 T4946 dobj 1,diluted
R3435 T4948 T4949 punct :,"5,000"
R3436 T4949 T4947 prep "5,000",1
R3437 T4950 T4946 prep in,diluted
R3438 T4951 T4952 compound blocking,solution
R3439 T4952 T4950 pobj solution,in
R3440 T4953 T4910 punct .,incubated
R3441 T4955 T4956 det The,membranes
R3442 T4956 T4957 nsubjpass membranes,washed
R3443 T4958 T4957 auxpass were,washed
R3444 T4959 T4957 advmod again,washed
R3445 T4960 T4961 nummod three,times
R3446 T4961 T4957 npadvmod times,washed
R3447 T4962 T4957 prep with,washed
R3448 T4963 T4964 nummod 1,TBS
R3449 T4964 T4962 pobj TBS,with
R3450 T4965 T4963 punct ×,1
R3451 T4966 T4964 punct /,TBS
R3452 T4967 T4968 nummod 0.1,%
R3453 T4968 T4969 compound %,Tween
R3454 T4969 T4964 appos Tween,TBS
R3455 T4970 T4969 punct -,Tween
R3456 T4971 T4969 nummod 20,Tween
R3457 T4972 T4957 punct ", ",washed
R3458 T4973 T4974 mark before,detected
R3459 T4974 T4957 advcl detected,washed
R3460 T4975 T4976 compound antibody,binding
R3461 T4976 T4974 nsubjpass binding,detected
R3462 T4977 T4974 auxpass was,detected
R3463 T4978 T4974 prep with,detected
R3464 T4979 T4980 amod enhanced,chemiluminescence
R3465 T4980 T4978 pobj chemiluminescence,with
R3466 T4981 T4982 punct (,PerkinElmer
R3467 T4982 T4974 parataxis PerkinElmer,detected
R3468 T4983 T4982 punct ", ",PerkinElmer
R3469 T4984 T4982 npadvmod Boston,PerkinElmer
R3470 T4985 T4982 punct ", ",PerkinElmer
R3471 T4986 T4982 npadvmod Massachusetts,PerkinElmer
R3472 T4987 T4982 punct ", ",PerkinElmer
R3473 T4988 T4989 compound United,States
R3474 T4989 T4982 npadvmod States,PerkinElmer
R3475 T4990 T4982 punct ),PerkinElmer
R3476 T4991 T4957 punct .,washed
R3477 T4993 T4994 amod Digital,images
R3478 T4994 T4995 nsubjpass images,captured
R3479 T4996 T4994 prep of,images
R3480 T4997 T4998 det each,blot
R3481 T4998 T4996 pobj blot,of
R3482 T4999 T4995 auxpass were,captured
R3483 T5000 T4995 prep with,captured
R3484 T5001 T5002 det a,system
R3485 T5002 T5000 pobj system,with
R3486 T5003 T5004 amod Molecular,Imager
R3487 T5004 T5005 compound Imager,FX
R3488 T5005 T5002 compound FX,system
R3489 T5006 T5007 compound gel,documentation
R3490 T5007 T5002 compound documentation,system
R3491 T5008 T4995 punct ", ",captured
R3492 T5009 T4995 cc and,captured
R3493 T5010 T5011 det the,intensity
R3494 T5011 T5012 nsubjpass intensity,quantified
R3495 T5012 T4995 conj quantified,captured
R3496 T5013 T5011 prep of,intensity
R3497 T5014 T5015 compound Aβ,staining
R3498 T5015 T5013 pobj staining,of
R3499 T5016 T5012 auxpass was,quantified
R3500 T5017 T5012 advcl using,quantified
R3501 T5018 T5019 nmod Quantity,software
R3502 T5019 T5017 dobj software,using
R3503 T5020 T5018 nummod One,Quantity
R3504 T5021 T5022 compound image,analysis
R3505 T5022 T5019 compound analysis,software
R3506 T5023 T5012 punct .,quantified