PMC:1283364 / 15431-16194 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T4358 0-10 NN denotes Thioflavin
T4360 10-11 HYPH denotes -
T4359 11-12 NN denotes S
T4361 13-21 NN denotes staining
T4362 21-207 sentence denotes Following deparaffinization of sections through xylene and alcohols, amyloid impregnation with thioflavin-S was performed according to the Guntern modification of the standard protocol.
T4363 22-31 VBG denotes Following
T4365 32-49 NN denotes deparaffinization
T4366 50-52 IN denotes of
T4367 53-61 NNS denotes sections
T4368 62-69 IN denotes through
T4369 70-76 NN denotes xylene
T4370 77-80 CC denotes and
T4371 81-89 NNS denotes alcohols
T4372 89-91 , denotes ,
T4373 91-98 NN denotes amyloid
T4374 99-111 NN denotes impregnation
T4375 112-116 IN denotes with
T4376 117-127 NN denotes thioflavin
T4378 127-128 HYPH denotes -
T4377 128-129 NN denotes S
T4379 130-133 VBD denotes was
T4364 134-143 VBN denotes performed
T4380 144-153 VBG denotes according
T4381 154-156 IN denotes to
T4382 157-160 DT denotes the
T4384 161-168 NN denotes Guntern
T4383 169-181 NN denotes modification
T4385 182-184 IN denotes of
T4386 185-188 DT denotes the
T4388 189-197 JJ denotes standard
T4387 198-206 NN denotes protocol
T4389 206-207 . denotes .
T4390 207-427 sentence denotes Slides holding 10-μm paraffin sections were washed twice in distilled water, then immersed for 5 min in a 0.25% potassium permanganate solution, followed by 5 min in a 1% potassium metabisulfate/1% oxalic acid solution.
T4391 208-214 NNS denotes Slides
T4393 215-222 VBG denotes holding
T4394 223-225 CD denotes 10
T4396 225-226 HYPH denotes -
T4395 226-228 NN denotes μm
T4398 229-237 NN denotes paraffin
T4397 238-246 NNS denotes sections
T4399 247-251 VBD denotes were
T4392 252-258 VBN denotes washed
T4400 259-264 RB denotes twice
T4401 265-267 IN denotes in
T4402 268-277 JJ denotes distilled
T4403 278-283 NN denotes water
T4404 283-285 , denotes ,
T4405 285-289 RB denotes then
T4406 290-298 VBN denotes immersed
T4407 299-302 IN denotes for
T4408 303-304 CD denotes 5
T4409 305-308 NN denotes min
T4410 309-311 IN denotes in
T4411 312-313 DT denotes a
T4413 314-318 CD denotes 0.25
T4414 318-319 NN denotes %
T4415 320-329 NN denotes potassium
T4416 330-342 NN denotes permanganate
T4412 343-351 NN denotes solution
T4417 351-353 , denotes ,
T4418 353-361 VBN denotes followed
T4419 362-364 IN denotes by
T4420 365-366 CD denotes 5
T4421 367-370 NN denotes min
T4422 371-373 IN denotes in
T4423 374-375 DT denotes a
T4425 376-377 CD denotes 1
T4426 377-378 NN denotes %
T4428 379-388 NN denotes potassium
T4427 389-402 NN denotes metabisulfate
T4429 402-403 HYPH denotes /
T4430 403-404 CD denotes 1
T4431 404-405 NN denotes %
T4433 406-412 JJ denotes oxalic
T4432 413-417 NN denotes acid
T4424 418-426 NN denotes solution
T4434 426-427 . denotes .
T4435 427-579 sentence denotes After this preparation, slides were placed into a filtered aqueous 0.02% thioflavin-S solution (Chroma-Gesellschaft Schmid, Kongen, Germany) for 8 min.
T4436 428-433 IN denotes After
T4438 434-438 DT denotes this
T4439 439-450 NN denotes preparation
T4440 450-452 , denotes ,
T4441 452-458 NNS denotes slides
T4442 459-463 VBD denotes were
T4437 464-470 VBN denotes placed
T4443 471-475 IN denotes into
T4444 476-477 DT denotes a
T4446 478-486 VBN denotes filtered
T4447 487-494 JJ denotes aqueous
T4448 495-499 CD denotes 0.02
T4449 499-500 NN denotes %
T4450 501-511 NN denotes thioflavin
T4452 511-512 HYPH denotes -
T4451 512-513 NN denotes S
T4445 514-522 NN denotes solution
T4453 523-524 -LRB- denotes (
T4455 524-530 NNP denotes Chroma
T4457 530-531 HYPH denotes -
T4456 531-543 NNP denotes Gesellschaft
T4454 544-550 NNP denotes Schmid
T4458 550-552 , denotes ,
T4459 552-558 NNP denotes Kongen
T4460 558-560 , denotes ,
T4461 560-567 NNP denotes Germany
T4462 567-568 -RRB- denotes )
T4463 569-572 IN denotes for
T4464 573-574 CD denotes 8
T4465 575-578 NN denotes min
T4466 578-579 . denotes .
T4467 579-763 sentence denotes Excess stain was removed by two brief rinses in 80% ethanol, then two in distilled water, after which slides were finished in aqueous mounting medium for florescence photomicrography.
T4468 580-586 JJ denotes Excess
T4469 587-592 NN denotes stain
T4471 593-596 VBD denotes was
T4470 597-604 VBN denotes removed
T4472 605-607 IN denotes by
T4473 608-611 CD denotes two
T4475 612-617 JJ denotes brief
T4474 618-624 NNS denotes rinses
T4476 625-627 IN denotes in
T4477 628-630 CD denotes 80
T4478 630-631 NN denotes %
T4479 632-639 NN denotes ethanol
T4480 639-641 , denotes ,
T4481 641-645 RB denotes then
T4482 646-649 CD denotes two
T4483 650-652 IN denotes in
T4484 653-662 JJ denotes distilled
T4485 663-668 NN denotes water
T4486 668-670 , denotes ,
T4487 670-675 IN denotes after
T4489 676-681 WDT denotes which
T4490 682-688 NNS denotes slides
T4491 689-693 VBD denotes were
T4488 694-702 VBN denotes finished
T4492 703-705 IN denotes in
T4493 706-713 JJ denotes aqueous
T4495 714-722 NN denotes mounting
T4494 723-729 NN denotes medium
T4496 730-733 IN denotes for
T4497 734-745 NN denotes florescence
T4498 746-762 NN denotes photomicrography
T4499 762-763 . denotes .
R2918 T4358 T4359 compound Thioflavin,S
R2919 T4359 T4361 compound S,staining
R2920 T4360 T4359 punct -,S
R2921 T4363 T4364 prep Following,performed
R2922 T4365 T4363 pobj deparaffinization,Following
R2923 T4366 T4365 prep of,deparaffinization
R2924 T4367 T4366 pobj sections,of
R2925 T4368 T4365 prep through,deparaffinization
R2926 T4369 T4368 pobj xylene,through
R2927 T4370 T4369 cc and,xylene
R2928 T4371 T4369 conj alcohols,xylene
R2929 T4372 T4364 punct ", ",performed
R2930 T4373 T4374 compound amyloid,impregnation
R2931 T4374 T4364 nsubjpass impregnation,performed
R2932 T4375 T4374 prep with,impregnation
R2933 T4376 T4377 compound thioflavin,S
R2934 T4377 T4375 pobj S,with
R2935 T4378 T4377 punct -,S
R2936 T4379 T4364 auxpass was,performed
R2937 T4380 T4364 prep according,performed
R2938 T4381 T4380 prep to,according
R2939 T4382 T4383 det the,modification
R2940 T4383 T4381 pobj modification,to
R2941 T4384 T4383 compound Guntern,modification
R2942 T4385 T4383 prep of,modification
R2943 T4386 T4387 det the,protocol
R2944 T4387 T4385 pobj protocol,of
R2945 T4388 T4387 amod standard,protocol
R2946 T4389 T4364 punct .,performed
R2947 T4391 T4392 nsubjpass Slides,washed
R2948 T4393 T4391 acl holding,Slides
R2949 T4394 T4395 nummod 10,μm
R2950 T4395 T4397 compound μm,sections
R2951 T4396 T4395 punct -,μm
R2952 T4397 T4393 dobj sections,holding
R2953 T4398 T4397 compound paraffin,sections
R2954 T4399 T4392 auxpass were,washed
R2955 T4400 T4392 advmod twice,washed
R2956 T4401 T4392 prep in,washed
R2957 T4402 T4403 amod distilled,water
R2958 T4403 T4401 pobj water,in
R2959 T4404 T4392 punct ", ",washed
R2960 T4405 T4406 advmod then,immersed
R2961 T4406 T4392 dep immersed,washed
R2962 T4407 T4406 prep for,immersed
R2963 T4408 T4409 nummod 5,min
R2964 T4409 T4407 pobj min,for
R2965 T4410 T4406 prep in,immersed
R2966 T4411 T4412 det a,solution
R2967 T4412 T4410 pobj solution,in
R2968 T4413 T4414 nummod 0.25,%
R2969 T4414 T4412 compound %,solution
R2970 T4415 T4412 compound potassium,solution
R2971 T4416 T4412 compound permanganate,solution
R2972 T4417 T4406 punct ", ",immersed
R2973 T4418 T4406 advcl followed,immersed
R2974 T4419 T4418 agent by,followed
R2975 T4420 T4421 nummod 5,min
R2976 T4421 T4419 pobj min,by
R2977 T4422 T4421 prep in,min
R2978 T4423 T4424 det a,solution
R2979 T4424 T4422 pobj solution,in
R2980 T4425 T4426 nummod 1,%
R2981 T4426 T4427 nmod %,metabisulfate
R2982 T4427 T4424 nmod metabisulfate,solution
R2983 T4428 T4427 nmod potassium,metabisulfate
R2984 T4429 T4427 punct /,metabisulfate
R2985 T4430 T4431 nummod 1,%
R2986 T4431 T4432 nmod %,acid
R2987 T4432 T4427 appos acid,metabisulfate
R2988 T4433 T4432 amod oxalic,acid
R2989 T4434 T4392 punct .,washed
R2990 T4436 T4437 prep After,placed
R2991 T4438 T4439 det this,preparation
R2992 T4439 T4436 pobj preparation,After
R2993 T4440 T4437 punct ", ",placed
R2994 T4441 T4437 nsubjpass slides,placed
R2995 T4442 T4437 auxpass were,placed
R2996 T4443 T4437 prep into,placed
R2997 T4444 T4445 det a,solution
R2998 T4445 T4443 pobj solution,into
R2999 T4446 T4445 amod filtered,solution
R3000 T4447 T4445 amod aqueous,solution
R3001 T4448 T4449 nummod 0.02,%
R3002 T4449 T4445 compound %,solution
R3003 T4450 T4451 compound thioflavin,S
R3004 T4451 T4445 compound S,solution
R3005 T4452 T4451 punct -,S
R3006 T4453 T4454 punct (,Schmid
R3007 T4454 T4445 parataxis Schmid,solution
R3008 T4455 T4456 compound Chroma,Gesellschaft
R3009 T4456 T4454 compound Gesellschaft,Schmid
R3010 T4457 T4456 punct -,Gesellschaft
R3011 T4458 T4454 punct ", ",Schmid
R3012 T4459 T4454 npadvmod Kongen,Schmid
R3013 T4460 T4454 punct ", ",Schmid
R3014 T4461 T4454 npadvmod Germany,Schmid
R3015 T4462 T4454 punct ),Schmid
R3016 T4463 T4437 prep for,placed
R3017 T4464 T4465 nummod 8,min
R3018 T4465 T4463 pobj min,for
R3019 T4466 T4437 punct .,placed
R3020 T4468 T4469 amod Excess,stain
R3021 T4469 T4470 nsubjpass stain,removed
R3022 T4471 T4470 auxpass was,removed
R3023 T4472 T4470 agent by,removed
R3024 T4473 T4474 nummod two,rinses
R3025 T4474 T4472 pobj rinses,by
R3026 T4475 T4474 amod brief,rinses
R3027 T4476 T4474 prep in,rinses
R3028 T4477 T4478 nummod 80,%
R3029 T4478 T4479 compound %,ethanol
R3030 T4479 T4476 pobj ethanol,in
R3031 T4480 T4474 punct ", ",rinses
R3032 T4481 T4482 advmod then,two
R3033 T4482 T4474 appos two,rinses
R3034 T4483 T4482 prep in,two
R3035 T4484 T4485 amod distilled,water
R3036 T4485 T4483 pobj water,in
R3037 T4486 T4474 punct ", ",rinses
R3038 T4487 T4488 prep after,finished
R3039 T4488 T4474 relcl finished,rinses
R3040 T4489 T4487 pobj which,after
R3041 T4490 T4488 nsubjpass slides,finished
R3042 T4491 T4488 auxpass were,finished
R3043 T4492 T4488 prep in,finished
R3044 T4493 T4494 amod aqueous,medium
R3045 T4494 T4492 pobj medium,in
R3046 T4495 T4494 compound mounting,medium
R3047 T4496 T4488 prep for,finished
R3048 T4497 T4498 compound florescence,photomicrography
R3049 T4498 T4496 pobj photomicrography,for
R3050 T4499 T4470 punct .,removed