PMC:1283364 / 12197-12722 5 Projects
Annnotations
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T3383 | 0-525 | sentence | denotes | Blots were blocked in PBS containing 5% nonfat dry milk powder, and incubated for 3 h at room temperature in blocking solution with one of the following antibodies: mouse monoclonal 22C11 (kind gift of Konrad Beyreuther and Andreas Weidemann; [17]) diluted 1:1,000, mouse monoclonal 6E10 (Signet Laboratories, Dedham, Massachusetts, United States) diluted 1:2,500, rabbit polyclonal anti-superoxide dismutase 1 (m/hSOD1) [18] diluted 1:2,500 to 1:4,000, or rabbit polyclonal CT15 (kind gift of Ed Koo; [19]) diluted 1:1,000. |
T3384 | 1-6 | NNS | denotes | Blots |
T3386 | 7-11 | VBD | denotes | were |
T3385 | 12-19 | VBN | denotes | blocked |
T3387 | 20-22 | IN | denotes | in |
T3388 | 23-26 | NN | denotes | PBS |
T3389 | 27-37 | VBG | denotes | containing |
T3390 | 38-39 | CD | denotes | 5 |
T3391 | 39-40 | NN | denotes | % |
T3393 | 41-47 | JJ | denotes | nonfat |
T3395 | 48-51 | JJ | denotes | dry |
T3394 | 52-56 | NN | denotes | milk |
T3392 | 57-63 | NN | denotes | powder |
T3396 | 63-65 | , | denotes | , |
T3397 | 65-68 | CC | denotes | and |
T3398 | 69-78 | VBN | denotes | incubated |
T3399 | 79-82 | IN | denotes | for |
T3400 | 83-84 | CD | denotes | 3 |
T3401 | 85-86 | NN | denotes | h |
T3402 | 87-89 | IN | denotes | at |
T3403 | 90-94 | NN | denotes | room |
T3404 | 95-106 | NN | denotes | temperature |
T3405 | 107-109 | IN | denotes | in |
T3406 | 110-118 | NN | denotes | blocking |
T3407 | 119-127 | NN | denotes | solution |
T3408 | 128-132 | IN | denotes | with |
T3409 | 133-136 | CD | denotes | one |
T3410 | 137-139 | IN | denotes | of |
T3411 | 140-143 | DT | denotes | the |
T3413 | 144-153 | VBG | denotes | following |
T3412 | 154-164 | NNS | denotes | antibodies |
T3414 | 164-166 | : | denotes | : |
T3415 | 166-171 | NN | denotes | mouse |
T3417 | 172-182 | JJ | denotes | monoclonal |
T3416 | 183-188 | NN | denotes | 22C11 |
T3418 | 189-190 | -LRB- | denotes | ( |
T3420 | 190-194 | JJ | denotes | kind |
T3421 | 195-199 | NN | denotes | gift |
T3422 | 200-202 | IN | denotes | of |
T3423 | 203-209 | NNP | denotes | Konrad |
T3424 | 210-220 | NNP | denotes | Beyreuther |
T3425 | 221-224 | CC | denotes | and |
T3426 | 225-232 | NNP | denotes | Andreas |
T3427 | 233-242 | NNP | denotes | Weidemann |
T3428 | 242-243 | : | denotes | ; |
T3429 | 244-245 | -LRB- | denotes | [ |
T3419 | 245-247 | CD | denotes | 17 |
T3430 | 247-248 | -RRB- | denotes | ] |
T3431 | 248-249 | -RRB- | denotes | ) |
T3432 | 250-257 | VBN | denotes | diluted |
T3433 | 258-259 | CD | denotes | 1 |
T3434 | 259-260 | SYM | denotes | : |
T3435 | 260-265 | CD | denotes | 1,000 |
T3436 | 265-267 | , | denotes | , |
T3437 | 267-272 | NN | denotes | mouse |
T3439 | 273-283 | JJ | denotes | monoclonal |
T3438 | 284-288 | NN | denotes | 6E10 |
T3440 | 289-290 | -LRB- | denotes | ( |
T3442 | 290-296 | NNP | denotes | Signet |
T3441 | 297-309 | NNP | denotes | Laboratories |
T3443 | 309-311 | , | denotes | , |
T3444 | 311-317 | NNP | denotes | Dedham |
T3445 | 317-319 | , | denotes | , |
T3446 | 319-332 | NNP | denotes | Massachusetts |
T3447 | 332-334 | , | denotes | , |
T3448 | 334-340 | NNP | denotes | United |
T3449 | 341-347 | NNP | denotes | States |
T3450 | 347-348 | -RRB- | denotes | ) |
T3451 | 349-356 | VBN | denotes | diluted |
T3452 | 357-358 | CD | denotes | 1 |
T3453 | 358-359 | SYM | denotes | : |
T3454 | 359-364 | CD | denotes | 2,500 |
T3455 | 364-366 | , | denotes | , |
T3456 | 366-372 | NN | denotes | rabbit |
T3458 | 373-383 | JJ | denotes | polyclonal |
T3459 | 384-399 | JJ | denotes | anti-superoxide |
T3460 | 400-409 | NN | denotes | dismutase |
T3461 | 410-411 | CD | denotes | 1 |
T3462 | 412-413 | -LRB- | denotes | ( |
T3463 | 413-414 | NN | denotes | m |
T3464 | 414-415 | HYPH | denotes | / |
T3457 | 415-420 | NN | denotes | hSOD1 |
T3465 | 420-421 | -RRB- | denotes | ) |
T3466 | 422-423 | -LRB- | denotes | [ |
T3467 | 423-425 | CD | denotes | 18 |
T3468 | 425-426 | -RRB- | denotes | ] |
T3469 | 427-434 | VBN | denotes | diluted |
T3470 | 435-436 | CD | denotes | 1 |
T3471 | 436-437 | SYM | denotes | : |
T3472 | 437-442 | CD | denotes | 2,500 |
T3473 | 443-445 | IN | denotes | to |
T3474 | 446-447 | CD | denotes | 1 |
T3475 | 447-448 | SYM | denotes | : |
T3476 | 448-453 | CD | denotes | 4,000 |
T3477 | 453-455 | , | denotes | , |
T3478 | 455-457 | CC | denotes | or |
T3479 | 458-464 | NN | denotes | rabbit |
T3481 | 465-475 | JJ | denotes | polyclonal |
T3480 | 476-480 | NN | denotes | CT15 |
T3482 | 481-482 | -LRB- | denotes | ( |
T3484 | 482-486 | JJ | denotes | kind |
T3483 | 487-491 | NN | denotes | gift |
T3485 | 492-494 | IN | denotes | of |
T3486 | 495-497 | NNP | denotes | Ed |
T3487 | 498-501 | NNP | denotes | Koo |
T3488 | 501-502 | : | denotes | ; |
T3489 | 503-504 | -LRB- | denotes | [ |
T3490 | 504-506 | CD | denotes | 19 |
T3491 | 506-507 | -RRB- | denotes | ] |
T3492 | 507-508 | -RRB- | denotes | ) |
T3493 | 509-516 | VBN | denotes | diluted |
T3494 | 517-518 | CD | denotes | 1 |
T3495 | 518-519 | SYM | denotes | : |
T3496 | 519-524 | CD | denotes | 1,000 |
T3497 | 524-525 | . | denotes | . |
R2293 | T3384 | T3385 | nsubjpass | Blots,blocked |
R2294 | T3386 | T3385 | auxpass | were,blocked |
R2295 | T3387 | T3385 | prep | in,blocked |
R2296 | T3388 | T3387 | pobj | PBS,in |
R2297 | T3389 | T3388 | acl | containing,PBS |
R2298 | T3390 | T3391 | nummod | 5,% |
R2299 | T3391 | T3392 | nmod | %,powder |
R2300 | T3392 | T3389 | dobj | powder,containing |
R2301 | T3393 | T3394 | amod | nonfat,milk |
R2302 | T3394 | T3392 | compound | milk,powder |
R2303 | T3395 | T3394 | amod | dry,milk |
R2304 | T3396 | T3385 | punct | ", ",blocked |
R2305 | T3397 | T3385 | cc | and,blocked |
R2306 | T3398 | T3385 | conj | incubated,blocked |
R2307 | T3399 | T3398 | prep | for,incubated |
R2308 | T3400 | T3401 | nummod | 3,h |
R2309 | T3401 | T3399 | pobj | h,for |
R2310 | T3402 | T3398 | prep | at,incubated |
R2311 | T3403 | T3404 | compound | room,temperature |
R2312 | T3404 | T3402 | pobj | temperature,at |
R2313 | T3405 | T3398 | prep | in,incubated |
R2314 | T3406 | T3407 | compound | blocking,solution |
R2315 | T3407 | T3405 | pobj | solution,in |
R2316 | T3408 | T3407 | prep | with,solution |
R2317 | T3409 | T3408 | pobj | one,with |
R2318 | T3410 | T3409 | prep | of,one |
R2319 | T3411 | T3412 | det | the,antibodies |
R2320 | T3412 | T3410 | pobj | antibodies,of |
R2321 | T3413 | T3412 | amod | following,antibodies |
R2322 | T3414 | T3412 | punct | : ,antibodies |
R2323 | T3415 | T3416 | nmod | mouse,22C11 |
R2324 | T3416 | T3412 | appos | 22C11,antibodies |
R2325 | T3417 | T3416 | amod | monoclonal,22C11 |
R2326 | T3418 | T3419 | punct | (,17 |
R2327 | T3419 | T3416 | parataxis | 17,22C11 |
R2328 | T3420 | T3421 | amod | kind,gift |
R2329 | T3421 | T3419 | dep | gift,17 |
R2330 | T3422 | T3421 | prep | of,gift |
R2331 | T3423 | T3424 | compound | Konrad,Beyreuther |
R2332 | T3424 | T3422 | pobj | Beyreuther,of |
R2333 | T3425 | T3424 | cc | and,Beyreuther |
R2334 | T3426 | T3427 | compound | Andreas,Weidemann |
R2335 | T3427 | T3424 | conj | Weidemann,Beyreuther |
R2336 | T3428 | T3419 | punct | ;,17 |
R2337 | T3429 | T3419 | punct | [,17 |
R2338 | T3430 | T3419 | punct | ],17 |
R2339 | T3431 | T3419 | punct | ),17 |
R2340 | T3432 | T3416 | acl | diluted,22C11 |
R2341 | T3433 | T3432 | npadvmod | 1,diluted |
R2342 | T3434 | T3435 | punct | :,"1,000" |
R2343 | T3435 | T3433 | prep | "1,000",1 |
R2344 | T3436 | T3416 | punct | ", ",22C11 |
R2345 | T3437 | T3438 | nmod | mouse,6E10 |
R2346 | T3438 | T3416 | conj | 6E10,22C11 |
R2347 | T3439 | T3438 | amod | monoclonal,6E10 |
R2348 | T3440 | T3441 | punct | (,Laboratories |
R2349 | T3441 | T3438 | parataxis | Laboratories,6E10 |
R2350 | T3442 | T3441 | compound | Signet,Laboratories |
R2351 | T3443 | T3441 | punct | ", ",Laboratories |
R2352 | T3444 | T3441 | npadvmod | Dedham,Laboratories |
R2353 | T3445 | T3441 | punct | ", ",Laboratories |
R2354 | T3446 | T3441 | npadvmod | Massachusetts,Laboratories |
R2355 | T3447 | T3441 | punct | ", ",Laboratories |
R2356 | T3448 | T3449 | compound | United,States |
R2357 | T3449 | T3441 | npadvmod | States,Laboratories |
R2358 | T3450 | T3441 | punct | ),Laboratories |
R2359 | T3451 | T3438 | acl | diluted,6E10 |
R2360 | T3452 | T3451 | npadvmod | 1,diluted |
R2361 | T3453 | T3454 | punct | :,"2,500" |
R2362 | T3454 | T3452 | prep | "2,500",1 |
R2363 | T3455 | T3438 | punct | ", ",6E10 |
R2364 | T3456 | T3457 | nmod | rabbit,hSOD1 |
R2365 | T3457 | T3438 | conj | hSOD1,6E10 |
R2366 | T3458 | T3457 | amod | polyclonal,hSOD1 |
R2367 | T3459 | T3457 | amod | anti-superoxide,hSOD1 |
R2368 | T3460 | T3457 | nmod | dismutase,hSOD1 |
R2369 | T3461 | T3457 | nummod | 1,hSOD1 |
R2370 | T3462 | T3457 | punct | (,hSOD1 |
R2371 | T3463 | T3457 | compound | m,hSOD1 |
R2372 | T3464 | T3457 | punct | /,hSOD1 |
R2373 | T3465 | T3457 | punct | ),hSOD1 |
R2374 | T3466 | T3467 | punct | [,18 |
R2375 | T3467 | T3457 | parataxis | 18,hSOD1 |
R2376 | T3468 | T3467 | punct | ],18 |
R2377 | T3469 | T3457 | acl | diluted,hSOD1 |
R2378 | T3470 | T3469 | npadvmod | 1,diluted |
R2379 | T3471 | T3472 | punct | :,"2,500" |
R2380 | T3472 | T3470 | prep | "2,500",1 |
R2381 | T3473 | T3470 | prep | to,1 |
R2382 | T3474 | T3473 | pobj | 1,to |
R2383 | T3475 | T3476 | punct | :,"4,000" |
R2384 | T3476 | T3474 | prep | "4,000",1 |
R2385 | T3477 | T3457 | punct | ", ",hSOD1 |
R2386 | T3478 | T3457 | cc | or,hSOD1 |
R2387 | T3479 | T3480 | nmod | rabbit,CT15 |
R2388 | T3480 | T3457 | conj | CT15,hSOD1 |
R2389 | T3481 | T3480 | amod | polyclonal,CT15 |
R2390 | T3482 | T3483 | punct | (,gift |
R2391 | T3483 | T3480 | parataxis | gift,CT15 |
R2392 | T3484 | T3483 | amod | kind,gift |
R2393 | T3485 | T3483 | prep | of,gift |
R2394 | T3486 | T3487 | compound | Ed,Koo |
R2395 | T3487 | T3485 | pobj | Koo,of |
R2396 | T3488 | T3483 | punct | ;,gift |
R2397 | T3489 | T3490 | punct | [,19 |
R2398 | T3490 | T3483 | parataxis | 19,gift |
R2399 | T3491 | T3490 | punct | ],19 |
R2400 | T3492 | T3483 | punct | ),gift |
R2401 | T3493 | T3480 | acl | diluted,CT15 |
R2402 | T3494 | T3493 | npadvmod | 1,diluted |
R2403 | T3495 | T3496 | punct | :,"1,000" |
R2404 | T3496 | T3494 | prep | "1,000",1 |
R2405 | T3497 | T3385 | punct | .,blocked |