PMC:1283364 / 12197-12722 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T3383 0-525 sentence denotes Blots were blocked in PBS containing 5% nonfat dry milk powder, and incubated for 3 h at room temperature in blocking solution with one of the following antibodies: mouse monoclonal 22C11 (kind gift of Konrad Beyreuther and Andreas Weidemann; [17]) diluted 1:1,000, mouse monoclonal 6E10 (Signet Laboratories, Dedham, Massachusetts, United States) diluted 1:2,500, rabbit polyclonal anti-superoxide dismutase 1 (m/hSOD1) [18] diluted 1:2,500 to 1:4,000, or rabbit polyclonal CT15 (kind gift of Ed Koo; [19]) diluted 1:1,000.
T3384 1-6 NNS denotes Blots
T3386 7-11 VBD denotes were
T3385 12-19 VBN denotes blocked
T3387 20-22 IN denotes in
T3388 23-26 NN denotes PBS
T3389 27-37 VBG denotes containing
T3390 38-39 CD denotes 5
T3391 39-40 NN denotes %
T3393 41-47 JJ denotes nonfat
T3395 48-51 JJ denotes dry
T3394 52-56 NN denotes milk
T3392 57-63 NN denotes powder
T3396 63-65 , denotes ,
T3397 65-68 CC denotes and
T3398 69-78 VBN denotes incubated
T3399 79-82 IN denotes for
T3400 83-84 CD denotes 3
T3401 85-86 NN denotes h
T3402 87-89 IN denotes at
T3403 90-94 NN denotes room
T3404 95-106 NN denotes temperature
T3405 107-109 IN denotes in
T3406 110-118 NN denotes blocking
T3407 119-127 NN denotes solution
T3408 128-132 IN denotes with
T3409 133-136 CD denotes one
T3410 137-139 IN denotes of
T3411 140-143 DT denotes the
T3413 144-153 VBG denotes following
T3412 154-164 NNS denotes antibodies
T3414 164-166 : denotes :
T3415 166-171 NN denotes mouse
T3417 172-182 JJ denotes monoclonal
T3416 183-188 NN denotes 22C11
T3418 189-190 -LRB- denotes (
T3420 190-194 JJ denotes kind
T3421 195-199 NN denotes gift
T3422 200-202 IN denotes of
T3423 203-209 NNP denotes Konrad
T3424 210-220 NNP denotes Beyreuther
T3425 221-224 CC denotes and
T3426 225-232 NNP denotes Andreas
T3427 233-242 NNP denotes Weidemann
T3428 242-243 : denotes ;
T3429 244-245 -LRB- denotes [
T3419 245-247 CD denotes 17
T3430 247-248 -RRB- denotes ]
T3431 248-249 -RRB- denotes )
T3432 250-257 VBN denotes diluted
T3433 258-259 CD denotes 1
T3434 259-260 SYM denotes :
T3435 260-265 CD denotes 1,000
T3436 265-267 , denotes ,
T3437 267-272 NN denotes mouse
T3439 273-283 JJ denotes monoclonal
T3438 284-288 NN denotes 6E10
T3440 289-290 -LRB- denotes (
T3442 290-296 NNP denotes Signet
T3441 297-309 NNP denotes Laboratories
T3443 309-311 , denotes ,
T3444 311-317 NNP denotes Dedham
T3445 317-319 , denotes ,
T3446 319-332 NNP denotes Massachusetts
T3447 332-334 , denotes ,
T3448 334-340 NNP denotes United
T3449 341-347 NNP denotes States
T3450 347-348 -RRB- denotes )
T3451 349-356 VBN denotes diluted
T3452 357-358 CD denotes 1
T3453 358-359 SYM denotes :
T3454 359-364 CD denotes 2,500
T3455 364-366 , denotes ,
T3456 366-372 NN denotes rabbit
T3458 373-383 JJ denotes polyclonal
T3459 384-399 JJ denotes anti-superoxide
T3460 400-409 NN denotes dismutase
T3461 410-411 CD denotes 1
T3462 412-413 -LRB- denotes (
T3463 413-414 NN denotes m
T3464 414-415 HYPH denotes /
T3457 415-420 NN denotes hSOD1
T3465 420-421 -RRB- denotes )
T3466 422-423 -LRB- denotes [
T3467 423-425 CD denotes 18
T3468 425-426 -RRB- denotes ]
T3469 427-434 VBN denotes diluted
T3470 435-436 CD denotes 1
T3471 436-437 SYM denotes :
T3472 437-442 CD denotes 2,500
T3473 443-445 IN denotes to
T3474 446-447 CD denotes 1
T3475 447-448 SYM denotes :
T3476 448-453 CD denotes 4,000
T3477 453-455 , denotes ,
T3478 455-457 CC denotes or
T3479 458-464 NN denotes rabbit
T3481 465-475 JJ denotes polyclonal
T3480 476-480 NN denotes CT15
T3482 481-482 -LRB- denotes (
T3484 482-486 JJ denotes kind
T3483 487-491 NN denotes gift
T3485 492-494 IN denotes of
T3486 495-497 NNP denotes Ed
T3487 498-501 NNP denotes Koo
T3488 501-502 : denotes ;
T3489 503-504 -LRB- denotes [
T3490 504-506 CD denotes 19
T3491 506-507 -RRB- denotes ]
T3492 507-508 -RRB- denotes )
T3493 509-516 VBN denotes diluted
T3494 517-518 CD denotes 1
T3495 518-519 SYM denotes :
T3496 519-524 CD denotes 1,000
T3497 524-525 . denotes .
R2293 T3384 T3385 nsubjpass Blots,blocked
R2294 T3386 T3385 auxpass were,blocked
R2295 T3387 T3385 prep in,blocked
R2296 T3388 T3387 pobj PBS,in
R2297 T3389 T3388 acl containing,PBS
R2298 T3390 T3391 nummod 5,%
R2299 T3391 T3392 nmod %,powder
R2300 T3392 T3389 dobj powder,containing
R2301 T3393 T3394 amod nonfat,milk
R2302 T3394 T3392 compound milk,powder
R2303 T3395 T3394 amod dry,milk
R2304 T3396 T3385 punct ", ",blocked
R2305 T3397 T3385 cc and,blocked
R2306 T3398 T3385 conj incubated,blocked
R2307 T3399 T3398 prep for,incubated
R2308 T3400 T3401 nummod 3,h
R2309 T3401 T3399 pobj h,for
R2310 T3402 T3398 prep at,incubated
R2311 T3403 T3404 compound room,temperature
R2312 T3404 T3402 pobj temperature,at
R2313 T3405 T3398 prep in,incubated
R2314 T3406 T3407 compound blocking,solution
R2315 T3407 T3405 pobj solution,in
R2316 T3408 T3407 prep with,solution
R2317 T3409 T3408 pobj one,with
R2318 T3410 T3409 prep of,one
R2319 T3411 T3412 det the,antibodies
R2320 T3412 T3410 pobj antibodies,of
R2321 T3413 T3412 amod following,antibodies
R2322 T3414 T3412 punct : ,antibodies
R2323 T3415 T3416 nmod mouse,22C11
R2324 T3416 T3412 appos 22C11,antibodies
R2325 T3417 T3416 amod monoclonal,22C11
R2326 T3418 T3419 punct (,17
R2327 T3419 T3416 parataxis 17,22C11
R2328 T3420 T3421 amod kind,gift
R2329 T3421 T3419 dep gift,17
R2330 T3422 T3421 prep of,gift
R2331 T3423 T3424 compound Konrad,Beyreuther
R2332 T3424 T3422 pobj Beyreuther,of
R2333 T3425 T3424 cc and,Beyreuther
R2334 T3426 T3427 compound Andreas,Weidemann
R2335 T3427 T3424 conj Weidemann,Beyreuther
R2336 T3428 T3419 punct ;,17
R2337 T3429 T3419 punct [,17
R2338 T3430 T3419 punct ],17
R2339 T3431 T3419 punct ),17
R2340 T3432 T3416 acl diluted,22C11
R2341 T3433 T3432 npadvmod 1,diluted
R2342 T3434 T3435 punct :,"1,000"
R2343 T3435 T3433 prep "1,000",1
R2344 T3436 T3416 punct ", ",22C11
R2345 T3437 T3438 nmod mouse,6E10
R2346 T3438 T3416 conj 6E10,22C11
R2347 T3439 T3438 amod monoclonal,6E10
R2348 T3440 T3441 punct (,Laboratories
R2349 T3441 T3438 parataxis Laboratories,6E10
R2350 T3442 T3441 compound Signet,Laboratories
R2351 T3443 T3441 punct ", ",Laboratories
R2352 T3444 T3441 npadvmod Dedham,Laboratories
R2353 T3445 T3441 punct ", ",Laboratories
R2354 T3446 T3441 npadvmod Massachusetts,Laboratories
R2355 T3447 T3441 punct ", ",Laboratories
R2356 T3448 T3449 compound United,States
R2357 T3449 T3441 npadvmod States,Laboratories
R2358 T3450 T3441 punct ),Laboratories
R2359 T3451 T3438 acl diluted,6E10
R2360 T3452 T3451 npadvmod 1,diluted
R2361 T3453 T3454 punct :,"2,500"
R2362 T3454 T3452 prep "2,500",1
R2363 T3455 T3438 punct ", ",6E10
R2364 T3456 T3457 nmod rabbit,hSOD1
R2365 T3457 T3438 conj hSOD1,6E10
R2366 T3458 T3457 amod polyclonal,hSOD1
R2367 T3459 T3457 amod anti-superoxide,hSOD1
R2368 T3460 T3457 nmod dismutase,hSOD1
R2369 T3461 T3457 nummod 1,hSOD1
R2370 T3462 T3457 punct (,hSOD1
R2371 T3463 T3457 compound m,hSOD1
R2372 T3464 T3457 punct /,hSOD1
R2373 T3465 T3457 punct ),hSOD1
R2374 T3466 T3467 punct [,18
R2375 T3467 T3457 parataxis 18,hSOD1
R2376 T3468 T3467 punct ],18
R2377 T3469 T3457 acl diluted,hSOD1
R2378 T3470 T3469 npadvmod 1,diluted
R2379 T3471 T3472 punct :,"2,500"
R2380 T3472 T3470 prep "2,500",1
R2381 T3473 T3470 prep to,1
R2382 T3474 T3473 pobj 1,to
R2383 T3475 T3476 punct :,"4,000"
R2384 T3476 T3474 prep "4,000",1
R2385 T3477 T3457 punct ", ",hSOD1
R2386 T3478 T3457 cc or,hSOD1
R2387 T3479 T3480 nmod rabbit,CT15
R2388 T3480 T3457 conj CT15,hSOD1
R2389 T3481 T3480 amod polyclonal,CT15
R2390 T3482 T3483 punct (,gift
R2391 T3483 T3480 parataxis gift,CT15
R2392 T3484 T3483 amod kind,gift
R2393 T3485 T3483 prep of,gift
R2394 T3486 T3487 compound Ed,Koo
R2395 T3487 T3485 pobj Koo,of
R2396 T3488 T3483 punct ;,gift
R2397 T3489 T3490 punct [,19
R2398 T3490 T3483 parataxis 19,gift
R2399 T3491 T3490 punct ],19
R2400 T3492 T3483 punct ),gift
R2401 T3493 T3480 acl diluted,CT15
R2402 T3494 T3493 npadvmod 1,diluted
R2403 T3495 T3496 punct :,"1,000"
R2404 T3496 T3494 prep "1,000",1
R2405 T3497 T3385 punct .,blocked