| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T3145 |
0-6 |
VBN |
denotes |
Frozen |
| T3147 |
7-15 |
JJ |
denotes |
cortical |
| T3148 |
16-18 |
CC |
denotes |
or |
| T3149 |
19-24 |
JJ |
denotes |
whole |
| T3150 |
25-34 |
NN |
denotes |
forebrain |
| T3146 |
35-41 |
NN |
denotes |
tissue |
| T3152 |
42-45 |
VBD |
denotes |
was |
| T3151 |
46-57 |
VBN |
denotes |
homogenized |
| T3153 |
58-60 |
IN |
denotes |
by |
| T3154 |
61-71 |
NN |
denotes |
sonication |
| T3155 |
72-74 |
IN |
denotes |
in |
| T3156 |
75-79 |
CD |
denotes |
five |
| T3157 |
80-87 |
NNS |
denotes |
volumes |
| T3158 |
88-90 |
IN |
denotes |
of |
| T3159 |
91-100 |
NN |
denotes |
phosphate |
| T3161 |
100-101 |
HYPH |
denotes |
- |
| T3160 |
101-109 |
VBN |
denotes |
buffered |
| T3162 |
110-116 |
NN |
denotes |
saline |
| T3163 |
117-118 |
-LRB- |
denotes |
( |
| T3164 |
118-121 |
NN |
denotes |
PBS |
| T3165 |
121-122 |
-RRB- |
denotes |
) |
| T3166 |
123-127 |
IN |
denotes |
with |
| T3167 |
128-129 |
CD |
denotes |
5 |
| T3168 |
130-132 |
NN |
denotes |
mM |
| T3170 |
133-137 |
NN |
denotes |
EDTA |
| T3171 |
138-141 |
CC |
denotes |
and |
| T3172 |
142-150 |
NN |
denotes |
protease |
| T3169 |
151-161 |
NNS |
denotes |
inhibitors |
| T3173 |
162-163 |
-LRB- |
denotes |
( |
| T3175 |
163-172 |
JJ |
denotes |
Mammalian |
| T3176 |
173-177 |
NN |
denotes |
cell |
| T3174 |
178-186 |
NN |
denotes |
cocktail |
| T3177 |
186-188 |
, |
denotes |
, |
| T3178 |
188-193 |
NNP |
denotes |
Sigma |
| T3179 |
193-195 |
, |
denotes |
, |
| T3180 |
195-198 |
NNP |
denotes |
St. |
| T3181 |
199-204 |
NNP |
denotes |
Louis |
| T3182 |
204-206 |
, |
denotes |
, |
| T3183 |
206-214 |
NNP |
denotes |
Missouri |
| T3184 |
214-216 |
, |
denotes |
, |
| T3185 |
216-222 |
NNP |
denotes |
United |
| T3186 |
223-229 |
NNP |
denotes |
States |
| T3187 |
229-230 |
-RRB- |
denotes |
) |
| T3188 |
230-232 |
, |
denotes |
, |
| T3189 |
232-237 |
VBG |
denotes |
using |
| T3190 |
238-239 |
DT |
denotes |
a |
| T3192 |
240-245 |
NN |
denotes |
probe |
| T3191 |
246-255 |
NN |
denotes |
sonicator |
| T3193 |
256-259 |
VBN |
denotes |
set |
| T3194 |
260-262 |
IN |
denotes |
to |
| T3195 |
263-265 |
CD |
denotes |
50 |
| T3196 |
265-266 |
NN |
denotes |
% |
| T3197 |
267-273 |
NN |
denotes |
output |
| T3198 |
274-275 |
-LRB- |
denotes |
( |
| T3199 |
275-281 |
NNP |
denotes |
TEKMAR |
| T3200 |
281-283 |
, |
denotes |
, |
| T3201 |
283-293 |
NNP |
denotes |
Cincinnati |
| T3202 |
293-295 |
, |
denotes |
, |
| T3203 |
295-299 |
NNP |
denotes |
Ohio |
| T3204 |
299-301 |
, |
denotes |
, |
| T3205 |
301-307 |
NNP |
denotes |
United |
| T3206 |
308-314 |
NNP |
denotes |
States |
| T3207 |
314-315 |
-RRB- |
denotes |
) |
| T3208 |
315-316 |
. |
denotes |
. |
| T3209 |
316-460 |
sentence |
denotes |
After dilution with an equal volume of PBS/EDTA/protease inhibitor, the samples were centrifuged briefly and the supernatant used for analysis. |
| T3210 |
317-322 |
IN |
denotes |
After |
| T3212 |
323-331 |
NN |
denotes |
dilution |
| T3213 |
332-336 |
IN |
denotes |
with |
| T3214 |
337-339 |
DT |
denotes |
an |
| T3216 |
340-345 |
JJ |
denotes |
equal |
| T3215 |
346-352 |
NN |
denotes |
volume |
| T3217 |
353-355 |
IN |
denotes |
of |
| T3218 |
356-359 |
NN |
denotes |
PBS |
| T3220 |
359-360 |
HYPH |
denotes |
/ |
| T3221 |
360-364 |
NN |
denotes |
EDTA |
| T3222 |
364-365 |
HYPH |
denotes |
/ |
| T3219 |
365-373 |
NN |
denotes |
protease |
| T3223 |
374-383 |
NN |
denotes |
inhibitor |
| T3224 |
383-385 |
, |
denotes |
, |
| T3225 |
385-388 |
DT |
denotes |
the |
| T3226 |
389-396 |
NNS |
denotes |
samples |
| T3227 |
397-401 |
VBD |
denotes |
were |
| T3211 |
402-413 |
VBN |
denotes |
centrifuged |
| T3228 |
414-421 |
RB |
denotes |
briefly |
| T3229 |
422-425 |
CC |
denotes |
and |
| T3230 |
426-429 |
DT |
denotes |
the |
| T3231 |
430-441 |
NN |
denotes |
supernatant |
| T3232 |
442-446 |
VBN |
denotes |
used |
| T3233 |
447-450 |
IN |
denotes |
for |
| T3234 |
451-459 |
NN |
denotes |
analysis |
| T3235 |
459-460 |
. |
denotes |
. |
| T3236 |
460-897 |
sentence |
denotes |
Fifty micrograms (6E10 and CT15) or 5 μg (22C11) of brain homogenate was loaded per lane onto 7.5%, 10%–20%, or 4%–20% Tris-HCl PAGE gels (Bio-Rad Laboratories, Hercules, California, United States) and electrophoresed for several hours in 1× Tris-glycine–sodium dodecyl sulfate (1×TG-SDS) buffer (6E10 and 22C11; Amresco, Solon, Ohio, United States) or 1× Tris-tricine-SDS buffer (CT15; Invitrogen, Carlsbad, California, United States). |
| T3237 |
461-466 |
CD |
denotes |
Fifty |
| T3238 |
467-477 |
NNS |
denotes |
micrograms |
| T3240 |
478-479 |
-LRB- |
denotes |
( |
| T3239 |
479-483 |
NN |
denotes |
6E10 |
| T3242 |
484-487 |
CC |
denotes |
and |
| T3243 |
488-492 |
NN |
denotes |
CT15 |
| T3244 |
492-493 |
-RRB- |
denotes |
) |
| T3245 |
494-496 |
CC |
denotes |
or |
| T3246 |
497-498 |
CD |
denotes |
5 |
| T3247 |
499-501 |
NN |
denotes |
μg |
| T3249 |
502-503 |
-LRB- |
denotes |
( |
| T3248 |
503-508 |
NN |
denotes |
22C11 |
| T3250 |
508-509 |
-RRB- |
denotes |
) |
| T3251 |
510-512 |
IN |
denotes |
of |
| T3252 |
513-518 |
NN |
denotes |
brain |
| T3253 |
519-529 |
NN |
denotes |
homogenate |
| T3254 |
530-533 |
VBD |
denotes |
was |
| T3241 |
534-540 |
VBN |
denotes |
loaded |
| T3255 |
541-544 |
IN |
denotes |
per |
| T3256 |
545-549 |
NN |
denotes |
lane |
| T3257 |
550-554 |
IN |
denotes |
onto |
| T3258 |
555-558 |
CD |
denotes |
7.5 |
| T3259 |
558-559 |
NN |
denotes |
% |
| T3261 |
559-561 |
, |
denotes |
, |
| T3262 |
561-563 |
CD |
denotes |
10 |
| T3263 |
563-564 |
NN |
denotes |
% |
| T3264 |
564-565 |
SYM |
denotes |
– |
| T3266 |
565-567 |
CD |
denotes |
20 |
| T3265 |
567-568 |
NN |
denotes |
% |
| T3267 |
568-570 |
, |
denotes |
, |
| T3268 |
570-572 |
CC |
denotes |
or |
| T3269 |
573-574 |
CD |
denotes |
4 |
| T3270 |
574-575 |
NN |
denotes |
% |
| T3271 |
575-576 |
SYM |
denotes |
– |
| T3273 |
576-578 |
CD |
denotes |
20 |
| T3272 |
578-579 |
NN |
denotes |
% |
| T3274 |
580-584 |
NN |
denotes |
Tris |
| T3275 |
584-585 |
HYPH |
denotes |
- |
| T3276 |
585-588 |
NN |
denotes |
HCl |
| T3260 |
589-593 |
NN |
denotes |
PAGE |
| T3277 |
594-598 |
NNS |
denotes |
gels |
| T3278 |
599-600 |
-LRB- |
denotes |
( |
| T3280 |
600-603 |
NNP |
denotes |
Bio |
| T3282 |
603-604 |
HYPH |
denotes |
- |
| T3281 |
604-607 |
NNP |
denotes |
Rad |
| T3279 |
608-620 |
NNP |
denotes |
Laboratories |
| T3283 |
620-622 |
, |
denotes |
, |
| T3284 |
622-630 |
NNP |
denotes |
Hercules |
| T3285 |
630-632 |
, |
denotes |
, |
| T3286 |
632-642 |
NNP |
denotes |
California |
| T3287 |
642-644 |
, |
denotes |
, |
| T3288 |
644-650 |
NNP |
denotes |
United |
| T3289 |
651-657 |
NNP |
denotes |
States |
| T3290 |
657-658 |
-RRB- |
denotes |
) |
| T3291 |
659-662 |
CC |
denotes |
and |
| T3292 |
663-678 |
VBN |
denotes |
electrophoresed |
| T3293 |
679-682 |
IN |
denotes |
for |
| T3294 |
683-690 |
JJ |
denotes |
several |
| T3295 |
691-696 |
NNS |
denotes |
hours |
| T3296 |
697-699 |
IN |
denotes |
in |
| T3297 |
700-701 |
CD |
denotes |
1 |
| T3299 |
701-702 |
SYM |
denotes |
× |
| T3300 |
703-707 |
NN |
denotes |
Tris |
| T3301 |
707-708 |
HYPH |
denotes |
- |
| T3302 |
708-715 |
NN |
denotes |
glycine |
| T3303 |
715-716 |
HYPH |
denotes |
– |
| T3304 |
716-722 |
NN |
denotes |
sodium |
| T3305 |
723-730 |
NN |
denotes |
dodecyl |
| T3298 |
731-738 |
NN |
denotes |
sulfate |
| T3307 |
739-740 |
-LRB- |
denotes |
( |
| T3308 |
740-741 |
CD |
denotes |
1 |
| T3310 |
741-742 |
SYM |
denotes |
× |
| T3311 |
742-744 |
NN |
denotes |
TG |
| T3312 |
744-745 |
HYPH |
denotes |
- |
| T3309 |
745-748 |
NN |
denotes |
SDS |
| T3313 |
748-749 |
-RRB- |
denotes |
) |
| T3306 |
750-756 |
NN |
denotes |
buffer |
| T3314 |
757-758 |
-LRB- |
denotes |
( |
| T3316 |
758-762 |
NN |
denotes |
6E10 |
| T3317 |
763-766 |
CC |
denotes |
and |
| T3318 |
767-772 |
NN |
denotes |
22C11 |
| T3319 |
772-773 |
: |
denotes |
; |
| T3315 |
774-781 |
NNP |
denotes |
Amresco |
| T3320 |
781-783 |
, |
denotes |
, |
| T3321 |
783-788 |
NNP |
denotes |
Solon |
| T3322 |
788-790 |
, |
denotes |
, |
| T3323 |
790-794 |
NNP |
denotes |
Ohio |
| T3324 |
794-796 |
, |
denotes |
, |
| T3325 |
796-802 |
NNP |
denotes |
United |
| T3326 |
803-809 |
NNP |
denotes |
States |
| T3327 |
809-810 |
-RRB- |
denotes |
) |
| T3328 |
811-813 |
CC |
denotes |
or |
| T3329 |
814-815 |
CD |
denotes |
1 |
| T3331 |
815-816 |
SYM |
denotes |
× |
| T3332 |
817-821 |
NN |
denotes |
Tris |
| T3334 |
821-822 |
HYPH |
denotes |
- |
| T3335 |
822-829 |
NN |
denotes |
tricine |
| T3336 |
829-830 |
HYPH |
denotes |
- |
| T3333 |
830-833 |
NN |
denotes |
SDS |
| T3330 |
834-840 |
NN |
denotes |
buffer |
| T3337 |
841-842 |
-LRB- |
denotes |
( |
| T3339 |
842-846 |
NN |
denotes |
CT15 |
| T3340 |
846-847 |
: |
denotes |
; |
| T3338 |
848-858 |
NNP |
denotes |
Invitrogen |
| T3341 |
858-860 |
, |
denotes |
, |
| T3342 |
860-868 |
NNP |
denotes |
Carlsbad |
| T3343 |
868-870 |
, |
denotes |
, |
| T3344 |
870-880 |
NNP |
denotes |
California |
| T3345 |
880-882 |
, |
denotes |
, |
| T3346 |
882-888 |
NNP |
denotes |
United |
| T3347 |
889-895 |
NNP |
denotes |
States |
| T3348 |
895-896 |
-RRB- |
denotes |
) |
| T3349 |
896-897 |
. |
denotes |
. |
| T3350 |
897-1057 |
sentence |
denotes |
Proteins were transferred overnight to 0.45-μm Optitran nitrocellulose (Schleicher and Schuell, Keene, New Hampshire, United States) in 1× TG buffer (Amresco). |
| T3351 |
898-906 |
NNS |
denotes |
Proteins |
| T3353 |
907-911 |
VBD |
denotes |
were |
| T3352 |
912-923 |
VBN |
denotes |
transferred |
| T3354 |
924-933 |
RB |
denotes |
overnight |
| T3355 |
934-936 |
IN |
denotes |
to |
| T3356 |
937-941 |
CD |
denotes |
0.45 |
| T3358 |
941-942 |
HYPH |
denotes |
- |
| T3357 |
942-944 |
NN |
denotes |
μm |
| T3360 |
945-953 |
NNP |
denotes |
Optitran |
| T3359 |
954-968 |
NN |
denotes |
nitrocellulose |
| T3361 |
969-970 |
-LRB- |
denotes |
( |
| T3362 |
970-980 |
NNP |
denotes |
Schleicher |
| T3363 |
981-984 |
CC |
denotes |
and |
| T3364 |
985-992 |
NNP |
denotes |
Schuell |
| T3365 |
992-994 |
, |
denotes |
, |
| T3366 |
994-999 |
NNP |
denotes |
Keene |
| T3367 |
999-1001 |
, |
denotes |
, |
| T3368 |
1001-1004 |
NNP |
denotes |
New |
| T3369 |
1005-1014 |
NNP |
denotes |
Hampshire |
| T3370 |
1014-1016 |
, |
denotes |
, |
| T3371 |
1016-1022 |
NNP |
denotes |
United |
| T3372 |
1023-1029 |
NNP |
denotes |
States |
| T3373 |
1029-1030 |
-RRB- |
denotes |
) |
| T3374 |
1031-1033 |
IN |
denotes |
in |
| T3375 |
1034-1035 |
CD |
denotes |
1 |
| T3377 |
1035-1036 |
SYM |
denotes |
× |
| T3378 |
1037-1039 |
NN |
denotes |
TG |
| T3376 |
1040-1046 |
NN |
denotes |
buffer |
| T3379 |
1047-1048 |
-LRB- |
denotes |
( |
| T3380 |
1048-1055 |
NNP |
denotes |
Amresco |
| T3381 |
1055-1056 |
-RRB- |
denotes |
) |
| T3382 |
1056-1057 |
. |
denotes |
. |
| T3383 |
1057-1582 |
sentence |
denotes |
Blots were blocked in PBS containing 5% nonfat dry milk powder, and incubated for 3 h at room temperature in blocking solution with one of the following antibodies: mouse monoclonal 22C11 (kind gift of Konrad Beyreuther and Andreas Weidemann; [17]) diluted 1:1,000, mouse monoclonal 6E10 (Signet Laboratories, Dedham, Massachusetts, United States) diluted 1:2,500, rabbit polyclonal anti-superoxide dismutase 1 (m/hSOD1) [18] diluted 1:2,500 to 1:4,000, or rabbit polyclonal CT15 (kind gift of Ed Koo; [19]) diluted 1:1,000. |
| T3384 |
1058-1063 |
NNS |
denotes |
Blots |
| T3386 |
1064-1068 |
VBD |
denotes |
were |
| T3385 |
1069-1076 |
VBN |
denotes |
blocked |
| T3387 |
1077-1079 |
IN |
denotes |
in |
| T3388 |
1080-1083 |
NN |
denotes |
PBS |
| T3389 |
1084-1094 |
VBG |
denotes |
containing |
| T3390 |
1095-1096 |
CD |
denotes |
5 |
| T3391 |
1096-1097 |
NN |
denotes |
% |
| T3393 |
1098-1104 |
JJ |
denotes |
nonfat |
| T3395 |
1105-1108 |
JJ |
denotes |
dry |
| T3394 |
1109-1113 |
NN |
denotes |
milk |
| T3392 |
1114-1120 |
NN |
denotes |
powder |
| T3396 |
1120-1122 |
, |
denotes |
, |
| T3397 |
1122-1125 |
CC |
denotes |
and |
| T3398 |
1126-1135 |
VBN |
denotes |
incubated |
| T3399 |
1136-1139 |
IN |
denotes |
for |
| T3400 |
1140-1141 |
CD |
denotes |
3 |
| T3401 |
1142-1143 |
NN |
denotes |
h |
| T3402 |
1144-1146 |
IN |
denotes |
at |
| T3403 |
1147-1151 |
NN |
denotes |
room |
| T3404 |
1152-1163 |
NN |
denotes |
temperature |
| T3405 |
1164-1166 |
IN |
denotes |
in |
| T3406 |
1167-1175 |
NN |
denotes |
blocking |
| T3407 |
1176-1184 |
NN |
denotes |
solution |
| T3408 |
1185-1189 |
IN |
denotes |
with |
| T3409 |
1190-1193 |
CD |
denotes |
one |
| T3410 |
1194-1196 |
IN |
denotes |
of |
| T3411 |
1197-1200 |
DT |
denotes |
the |
| T3413 |
1201-1210 |
VBG |
denotes |
following |
| T3412 |
1211-1221 |
NNS |
denotes |
antibodies |
| T3414 |
1221-1223 |
: |
denotes |
: |
| T3415 |
1223-1228 |
NN |
denotes |
mouse |
| T3417 |
1229-1239 |
JJ |
denotes |
monoclonal |
| T3416 |
1240-1245 |
NN |
denotes |
22C11 |
| T3418 |
1246-1247 |
-LRB- |
denotes |
( |
| T3420 |
1247-1251 |
JJ |
denotes |
kind |
| T3421 |
1252-1256 |
NN |
denotes |
gift |
| T3422 |
1257-1259 |
IN |
denotes |
of |
| T3423 |
1260-1266 |
NNP |
denotes |
Konrad |
| T3424 |
1267-1277 |
NNP |
denotes |
Beyreuther |
| T3425 |
1278-1281 |
CC |
denotes |
and |
| T3426 |
1282-1289 |
NNP |
denotes |
Andreas |
| T3427 |
1290-1299 |
NNP |
denotes |
Weidemann |
| T3428 |
1299-1300 |
: |
denotes |
; |
| T3429 |
1301-1302 |
-LRB- |
denotes |
[ |
| T3419 |
1302-1304 |
CD |
denotes |
17 |
| T3430 |
1304-1305 |
-RRB- |
denotes |
] |
| T3431 |
1305-1306 |
-RRB- |
denotes |
) |
| T3432 |
1307-1314 |
VBN |
denotes |
diluted |
| T3433 |
1315-1316 |
CD |
denotes |
1 |
| T3434 |
1316-1317 |
SYM |
denotes |
: |
| T3435 |
1317-1322 |
CD |
denotes |
1,000 |
| T3436 |
1322-1324 |
, |
denotes |
, |
| T3437 |
1324-1329 |
NN |
denotes |
mouse |
| T3439 |
1330-1340 |
JJ |
denotes |
monoclonal |
| T3438 |
1341-1345 |
NN |
denotes |
6E10 |
| T3440 |
1346-1347 |
-LRB- |
denotes |
( |
| T3442 |
1347-1353 |
NNP |
denotes |
Signet |
| T3441 |
1354-1366 |
NNP |
denotes |
Laboratories |
| T3443 |
1366-1368 |
, |
denotes |
, |
| T3444 |
1368-1374 |
NNP |
denotes |
Dedham |
| T3445 |
1374-1376 |
, |
denotes |
, |
| T3446 |
1376-1389 |
NNP |
denotes |
Massachusetts |
| T3447 |
1389-1391 |
, |
denotes |
, |
| T3448 |
1391-1397 |
NNP |
denotes |
United |
| T3449 |
1398-1404 |
NNP |
denotes |
States |
| T3450 |
1404-1405 |
-RRB- |
denotes |
) |
| T3451 |
1406-1413 |
VBN |
denotes |
diluted |
| T3452 |
1414-1415 |
CD |
denotes |
1 |
| T3453 |
1415-1416 |
SYM |
denotes |
: |
| T3454 |
1416-1421 |
CD |
denotes |
2,500 |
| T3455 |
1421-1423 |
, |
denotes |
, |
| T3456 |
1423-1429 |
NN |
denotes |
rabbit |
| T3458 |
1430-1440 |
JJ |
denotes |
polyclonal |
| T3459 |
1441-1456 |
JJ |
denotes |
anti-superoxide |
| T3460 |
1457-1466 |
NN |
denotes |
dismutase |
| T3461 |
1467-1468 |
CD |
denotes |
1 |
| T3462 |
1469-1470 |
-LRB- |
denotes |
( |
| T3463 |
1470-1471 |
NN |
denotes |
m |
| T3464 |
1471-1472 |
HYPH |
denotes |
/ |
| T3457 |
1472-1477 |
NN |
denotes |
hSOD1 |
| T3465 |
1477-1478 |
-RRB- |
denotes |
) |
| T3466 |
1479-1480 |
-LRB- |
denotes |
[ |
| T3467 |
1480-1482 |
CD |
denotes |
18 |
| T3468 |
1482-1483 |
-RRB- |
denotes |
] |
| T3469 |
1484-1491 |
VBN |
denotes |
diluted |
| T3470 |
1492-1493 |
CD |
denotes |
1 |
| T3471 |
1493-1494 |
SYM |
denotes |
: |
| T3472 |
1494-1499 |
CD |
denotes |
2,500 |
| T3473 |
1500-1502 |
IN |
denotes |
to |
| T3474 |
1503-1504 |
CD |
denotes |
1 |
| T3475 |
1504-1505 |
SYM |
denotes |
: |
| T3476 |
1505-1510 |
CD |
denotes |
4,000 |
| T3477 |
1510-1512 |
, |
denotes |
, |
| T3478 |
1512-1514 |
CC |
denotes |
or |
| T3479 |
1515-1521 |
NN |
denotes |
rabbit |
| T3481 |
1522-1532 |
JJ |
denotes |
polyclonal |
| T3480 |
1533-1537 |
NN |
denotes |
CT15 |
| T3482 |
1538-1539 |
-LRB- |
denotes |
( |
| T3484 |
1539-1543 |
JJ |
denotes |
kind |
| T3483 |
1544-1548 |
NN |
denotes |
gift |
| T3485 |
1549-1551 |
IN |
denotes |
of |
| T3486 |
1552-1554 |
NNP |
denotes |
Ed |
| T3487 |
1555-1558 |
NNP |
denotes |
Koo |
| T3488 |
1558-1559 |
: |
denotes |
; |
| T3489 |
1560-1561 |
-LRB- |
denotes |
[ |
| T3490 |
1561-1563 |
CD |
denotes |
19 |
| T3491 |
1563-1564 |
-RRB- |
denotes |
] |
| T3492 |
1564-1565 |
-RRB- |
denotes |
) |
| T3493 |
1566-1573 |
VBN |
denotes |
diluted |
| T3494 |
1574-1575 |
CD |
denotes |
1 |
| T3495 |
1575-1576 |
SYM |
denotes |
: |
| T3496 |
1576-1581 |
CD |
denotes |
1,000 |
| T3497 |
1581-1582 |
. |
denotes |
. |
| T3498 |
1582-1793 |
sentence |
denotes |
Subsequently, the blots were washed with PBS containing 0.1% Tween-20, and then incubated with either goat anti-mouse– or goat anti-rabbit–HRP conjugated secondary antibody diluted 1:1,000 in blocking solution. |
| T3499 |
1583-1595 |
RB |
denotes |
Subsequently |
| T3501 |
1595-1597 |
, |
denotes |
, |
| T3502 |
1597-1600 |
DT |
denotes |
the |
| T3503 |
1601-1606 |
NNS |
denotes |
blots |
| T3504 |
1607-1611 |
VBD |
denotes |
were |
| T3500 |
1612-1618 |
VBN |
denotes |
washed |
| T3505 |
1619-1623 |
IN |
denotes |
with |
| T3506 |
1624-1627 |
NN |
denotes |
PBS |
| T3507 |
1628-1638 |
VBG |
denotes |
containing |
| T3508 |
1639-1642 |
CD |
denotes |
0.1 |
| T3509 |
1642-1643 |
NN |
denotes |
% |
| T3510 |
1644-1649 |
NN |
denotes |
Tween |
| T3511 |
1649-1650 |
HYPH |
denotes |
- |
| T3512 |
1650-1652 |
CD |
denotes |
20 |
| T3513 |
1652-1654 |
, |
denotes |
, |
| T3514 |
1654-1657 |
CC |
denotes |
and |
| T3515 |
1658-1662 |
RB |
denotes |
then |
| T3516 |
1663-1672 |
VBN |
denotes |
incubated |
| T3517 |
1673-1677 |
IN |
denotes |
with |
| T3518 |
1678-1684 |
CC |
denotes |
either |
| T3519 |
1685-1689 |
NN |
denotes |
goat |
| T3521 |
1690-1700 |
JJ |
denotes |
anti-mouse |
| T3522 |
1700-1701 |
HYPH |
denotes |
– |
| T3523 |
1702-1704 |
CC |
denotes |
or |
| T3524 |
1705-1709 |
NN |
denotes |
goat |
| T3525 |
1710-1721 |
JJ |
denotes |
anti-rabbit |
| T3526 |
1721-1722 |
HYPH |
denotes |
– |
| T3527 |
1722-1725 |
NN |
denotes |
HRP |
| T3528 |
1726-1736 |
VBN |
denotes |
conjugated |
| T3529 |
1737-1746 |
JJ |
denotes |
secondary |
| T3520 |
1747-1755 |
NN |
denotes |
antibody |
| T3530 |
1756-1763 |
VBN |
denotes |
diluted |
| T3531 |
1764-1765 |
CD |
denotes |
1 |
| T3532 |
1765-1766 |
SYM |
denotes |
: |
| T3533 |
1766-1771 |
CD |
denotes |
1,000 |
| T3534 |
1772-1774 |
IN |
denotes |
in |
| T3535 |
1775-1783 |
NN |
denotes |
blocking |
| T3536 |
1784-1792 |
NN |
denotes |
solution |
| T3537 |
1792-1793 |
. |
denotes |
. |
| T3538 |
1793-1969 |
sentence |
denotes |
After several additional rinses in PBS with 0.1% Tween-20, blots were developed with enhanced chemiluminescence reagent and imaged with the Bio-Rad Molecular Imager FX system. |
| T3539 |
1794-1799 |
IN |
denotes |
After |
| T3541 |
1800-1807 |
JJ |
denotes |
several |
| T3543 |
1808-1818 |
JJ |
denotes |
additional |
| T3542 |
1819-1825 |
NNS |
denotes |
rinses |
| T3544 |
1826-1828 |
IN |
denotes |
in |
| T3545 |
1829-1832 |
NN |
denotes |
PBS |
| T3546 |
1833-1837 |
IN |
denotes |
with |
| T3547 |
1838-1841 |
CD |
denotes |
0.1 |
| T3549 |
1841-1842 |
NN |
denotes |
% |
| T3548 |
1843-1848 |
NN |
denotes |
Tween |
| T3550 |
1848-1849 |
HYPH |
denotes |
- |
| T3551 |
1849-1851 |
CD |
denotes |
20 |
| T3552 |
1851-1853 |
, |
denotes |
, |
| T3553 |
1853-1858 |
NNS |
denotes |
blots |
| T3554 |
1859-1863 |
VBD |
denotes |
were |
| T3540 |
1864-1873 |
VBN |
denotes |
developed |
| T3555 |
1874-1878 |
IN |
denotes |
with |
| T3556 |
1879-1887 |
VBN |
denotes |
enhanced |
| T3558 |
1888-1905 |
NN |
denotes |
chemiluminescence |
| T3557 |
1906-1913 |
NN |
denotes |
reagent |
| T3559 |
1914-1917 |
CC |
denotes |
and |
| T3560 |
1918-1924 |
VBN |
denotes |
imaged |
| T3561 |
1925-1929 |
IN |
denotes |
with |
| T3562 |
1930-1933 |
DT |
denotes |
the |
| T3564 |
1934-1937 |
NNP |
denotes |
Bio |
| T3566 |
1937-1938 |
HYPH |
denotes |
- |
| T3565 |
1938-1941 |
NNP |
denotes |
Rad |
| T3568 |
1942-1951 |
NNP |
denotes |
Molecular |
| T3569 |
1952-1958 |
NNP |
denotes |
Imager |
| T3567 |
1959-1961 |
NNP |
denotes |
FX |
| T3563 |
1962-1968 |
NN |
denotes |
system |
| T3570 |
1968-1969 |
. |
denotes |
. |
| R2062 |
T3145 |
T3146 |
amod |
Frozen,tissue |
| R2063 |
T3146 |
T3151 |
nsubjpass |
tissue,homogenized |
| R2064 |
T3147 |
T3146 |
amod |
cortical,tissue |
| R2065 |
T3148 |
T3147 |
cc |
or,cortical |
| R2066 |
T3149 |
T3150 |
amod |
whole,forebrain |
| R2067 |
T3150 |
T3147 |
conj |
forebrain,cortical |
| R2068 |
T3152 |
T3151 |
auxpass |
was,homogenized |
| R2069 |
T3153 |
T3151 |
prep |
by,homogenized |
| R2070 |
T3154 |
T3153 |
pobj |
sonication,by |
| R2071 |
T3155 |
T3151 |
prep |
in,homogenized |
| R2072 |
T3156 |
T3157 |
nummod |
five,volumes |
| R2073 |
T3157 |
T3155 |
pobj |
volumes,in |
| R2074 |
T3158 |
T3157 |
prep |
of,volumes |
| R2075 |
T3159 |
T3160 |
npadvmod |
phosphate,buffered |
| R2076 |
T3160 |
T3162 |
amod |
buffered,saline |
| R2077 |
T3161 |
T3160 |
punct |
-,buffered |
| R2078 |
T3162 |
T3158 |
pobj |
saline,of |
| R2079 |
T3163 |
T3162 |
punct |
(,saline |
| R2080 |
T3164 |
T3162 |
appos |
PBS,saline |
| R2081 |
T3165 |
T3151 |
punct |
),homogenized |
| R2082 |
T3166 |
T3151 |
prep |
with,homogenized |
| R2083 |
T3167 |
T3168 |
nummod |
5,mM |
| R2084 |
T3168 |
T3169 |
nmod |
mM,inhibitors |
| R2085 |
T3169 |
T3166 |
pobj |
inhibitors,with |
| R2086 |
T3170 |
T3169 |
nmod |
EDTA,inhibitors |
| R2087 |
T3171 |
T3170 |
cc |
and,EDTA |
| R2088 |
T3172 |
T3170 |
conj |
protease,EDTA |
| R2089 |
T3173 |
T3174 |
punct |
(,cocktail |
| R2090 |
T3174 |
T3169 |
parataxis |
cocktail,inhibitors |
| R2091 |
T3175 |
T3174 |
amod |
Mammalian,cocktail |
| R2092 |
T3176 |
T3174 |
compound |
cell,cocktail |
| R2093 |
T3177 |
T3174 |
punct |
", ",cocktail |
| R2094 |
T3178 |
T3174 |
npadvmod |
Sigma,cocktail |
| R2095 |
T3179 |
T3174 |
punct |
", ",cocktail |
| R2096 |
T3180 |
T3181 |
compound |
St.,Louis |
| R2097 |
T3181 |
T3174 |
npadvmod |
Louis,cocktail |
| R2098 |
T3182 |
T3174 |
punct |
", ",cocktail |
| R2099 |
T3183 |
T3174 |
npadvmod |
Missouri,cocktail |
| R2100 |
T3184 |
T3174 |
punct |
", ",cocktail |
| R2101 |
T3185 |
T3186 |
compound |
United,States |
| R2102 |
T3186 |
T3174 |
npadvmod |
States,cocktail |
| R2103 |
T3187 |
T3174 |
punct |
),cocktail |
| R2104 |
T3188 |
T3151 |
punct |
", ",homogenized |
| R2105 |
T3189 |
T3151 |
advcl |
using,homogenized |
| R2106 |
T3190 |
T3191 |
det |
a,sonicator |
| R2107 |
T3191 |
T3189 |
dobj |
sonicator,using |
| R2108 |
T3192 |
T3191 |
compound |
probe,sonicator |
| R2109 |
T3193 |
T3191 |
acl |
set,sonicator |
| R2110 |
T3194 |
T3193 |
prep |
to,set |
| R2111 |
T3195 |
T3196 |
nummod |
50,% |
| R2112 |
T3196 |
T3197 |
compound |
%,output |
| R2113 |
T3197 |
T3194 |
pobj |
output,to |
| R2114 |
T3198 |
T3199 |
punct |
(,TEKMAR |
| R2115 |
T3199 |
T3191 |
parataxis |
TEKMAR,sonicator |
| R2116 |
T3200 |
T3199 |
punct |
", ",TEKMAR |
| R2117 |
T3201 |
T3199 |
npadvmod |
Cincinnati,TEKMAR |
| R2118 |
T3202 |
T3199 |
punct |
", ",TEKMAR |
| R2119 |
T3203 |
T3199 |
npadvmod |
Ohio,TEKMAR |
| R2120 |
T3204 |
T3199 |
punct |
", ",TEKMAR |
| R2121 |
T3205 |
T3206 |
compound |
United,States |
| R2122 |
T3206 |
T3199 |
npadvmod |
States,TEKMAR |
| R2123 |
T3207 |
T3199 |
punct |
),TEKMAR |
| R2124 |
T3208 |
T3151 |
punct |
.,homogenized |
| R2125 |
T3210 |
T3211 |
prep |
After,centrifuged |
| R2126 |
T3212 |
T3210 |
pobj |
dilution,After |
| R2127 |
T3213 |
T3212 |
prep |
with,dilution |
| R2128 |
T3214 |
T3215 |
det |
an,volume |
| R2129 |
T3215 |
T3213 |
pobj |
volume,with |
| R2130 |
T3216 |
T3215 |
amod |
equal,volume |
| R2131 |
T3217 |
T3215 |
prep |
of,volume |
| R2132 |
T3218 |
T3219 |
compound |
PBS,protease |
| R2133 |
T3219 |
T3223 |
compound |
protease,inhibitor |
| R2134 |
T3220 |
T3219 |
punct |
/,protease |
| R2135 |
T3221 |
T3219 |
compound |
EDTA,protease |
| R2136 |
T3222 |
T3219 |
punct |
/,protease |
| R2137 |
T3223 |
T3217 |
pobj |
inhibitor,of |
| R2138 |
T3224 |
T3211 |
punct |
", ",centrifuged |
| R2139 |
T3225 |
T3226 |
det |
the,samples |
| R2140 |
T3226 |
T3211 |
nsubjpass |
samples,centrifuged |
| R2141 |
T3227 |
T3211 |
auxpass |
were,centrifuged |
| R2142 |
T3228 |
T3211 |
advmod |
briefly,centrifuged |
| R2143 |
T3229 |
T3211 |
cc |
and,centrifuged |
| R2144 |
T3230 |
T3231 |
det |
the,supernatant |
| R2145 |
T3231 |
T3232 |
nsubj |
supernatant,used |
| R2146 |
T3232 |
T3211 |
conj |
used,centrifuged |
| R2147 |
T3233 |
T3232 |
prep |
for,used |
| R2148 |
T3234 |
T3233 |
pobj |
analysis,for |
| R2149 |
T3235 |
T3211 |
punct |
.,centrifuged |
| R2150 |
T3237 |
T3238 |
nummod |
Fifty,micrograms |
| R2151 |
T3238 |
T3239 |
nmod |
micrograms,6E10 |
| R2152 |
T3239 |
T3241 |
nsubjpass |
6E10,loaded |
| R2153 |
T3240 |
T3239 |
punct |
(,6E10 |
| R2154 |
T3242 |
T3239 |
cc |
and,6E10 |
| R2155 |
T3243 |
T3239 |
conj |
CT15,6E10 |
| R2156 |
T3244 |
T3243 |
punct |
),CT15 |
| R2157 |
T3245 |
T3239 |
cc |
or,6E10 |
| R2158 |
T3246 |
T3247 |
nummod |
5,μg |
| R2159 |
T3247 |
T3248 |
nmod |
μg,22C11 |
| R2160 |
T3248 |
T3239 |
conj |
22C11,6E10 |
| R2161 |
T3249 |
T3248 |
punct |
(,22C11 |
| R2162 |
T3250 |
T3248 |
punct |
),22C11 |
| R2163 |
T3251 |
T3239 |
prep |
of,6E10 |
| R2164 |
T3252 |
T3253 |
compound |
brain,homogenate |
| R2165 |
T3253 |
T3251 |
pobj |
homogenate,of |
| R2166 |
T3254 |
T3241 |
auxpass |
was,loaded |
| R2167 |
T3255 |
T3241 |
prep |
per,loaded |
| R2168 |
T3256 |
T3255 |
pobj |
lane,per |
| R2169 |
T3257 |
T3241 |
prep |
onto,loaded |
| R2170 |
T3258 |
T3259 |
nummod |
7.5,% |
| R2171 |
T3259 |
T3260 |
nmod |
%,PAGE |
| R2172 |
T3260 |
T3277 |
compound |
PAGE,gels |
| R2173 |
T3261 |
T3259 |
punct |
", ",% |
| R2174 |
T3262 |
T3263 |
nummod |
10,% |
| R2175 |
T3263 |
T3259 |
conj |
%,% |
| R2176 |
T3264 |
T3265 |
punct |
–,% |
| R2177 |
T3265 |
T3263 |
prep |
%,% |
| R2178 |
T3266 |
T3265 |
nummod |
20,% |
| R2179 |
T3267 |
T3263 |
punct |
", ",% |
| R2180 |
T3268 |
T3263 |
cc |
or,% |
| R2181 |
T3269 |
T3270 |
nummod |
4,% |
| R2182 |
T3270 |
T3263 |
conj |
%,% |
| R2183 |
T3271 |
T3272 |
punct |
–,% |
| R2184 |
T3272 |
T3270 |
prep |
%,% |
| R2185 |
T3273 |
T3272 |
nummod |
20,% |
| R2186 |
T3274 |
T3260 |
compound |
Tris,PAGE |
| R2187 |
T3275 |
T3260 |
punct |
-,PAGE |
| R2188 |
T3276 |
T3260 |
compound |
HCl,PAGE |
| R2189 |
T3277 |
T3257 |
pobj |
gels,onto |
| R2190 |
T3278 |
T3279 |
punct |
(,Laboratories |
| R2191 |
T3279 |
T3277 |
parataxis |
Laboratories,gels |
| R2192 |
T3280 |
T3281 |
compound |
Bio,Rad |
| R2193 |
T3281 |
T3279 |
compound |
Rad,Laboratories |
| R2194 |
T3282 |
T3281 |
punct |
-,Rad |
| R2195 |
T3283 |
T3279 |
punct |
", ",Laboratories |
| R2196 |
T3284 |
T3279 |
npadvmod |
Hercules,Laboratories |
| R2197 |
T3285 |
T3279 |
punct |
", ",Laboratories |
| R2198 |
T3286 |
T3279 |
npadvmod |
California,Laboratories |
| R2199 |
T3287 |
T3279 |
punct |
", ",Laboratories |
| R2200 |
T3288 |
T3289 |
compound |
United,States |
| R2201 |
T3289 |
T3279 |
npadvmod |
States,Laboratories |
| R2202 |
T3290 |
T3279 |
punct |
),Laboratories |
| R2203 |
T3291 |
T3241 |
cc |
and,loaded |
| R2204 |
T3292 |
T3241 |
conj |
electrophoresed,loaded |
| R2205 |
T3293 |
T3292 |
prep |
for,electrophoresed |
| R2206 |
T3294 |
T3295 |
amod |
several,hours |
| R2207 |
T3295 |
T3293 |
pobj |
hours,for |
| R2208 |
T3296 |
T3292 |
prep |
in,electrophoresed |
| R2209 |
T3297 |
T3298 |
nummod |
1,sulfate |
| R2210 |
T3298 |
T3306 |
nmod |
sulfate,buffer |
| R2211 |
T3299 |
T3297 |
punct |
×,1 |
| R2212 |
T3300 |
T3298 |
nmod |
Tris,sulfate |
| R2213 |
T3301 |
T3298 |
punct |
-,sulfate |
| R2214 |
T3302 |
T3298 |
nmod |
glycine,sulfate |
| R2215 |
T3303 |
T3298 |
punct |
–,sulfate |
| R2216 |
T3304 |
T3298 |
nmod |
sodium,sulfate |
| R2217 |
T3305 |
T3298 |
nmod |
dodecyl,sulfate |
| R2218 |
T3306 |
T3296 |
pobj |
buffer,in |
| R2219 |
T3307 |
T3298 |
punct |
(,sulfate |
| R2220 |
T3308 |
T3309 |
nummod |
1,SDS |
| R2221 |
T3309 |
T3298 |
appos |
SDS,sulfate |
| R2222 |
T3310 |
T3308 |
punct |
×,1 |
| R2223 |
T3311 |
T3309 |
compound |
TG,SDS |
| R2224 |
T3312 |
T3309 |
punct |
-,SDS |
| R2225 |
T3313 |
T3306 |
punct |
),buffer |
| R2226 |
T3314 |
T3315 |
punct |
(,Amresco |
| R2227 |
T3315 |
T3306 |
parataxis |
Amresco,buffer |
| R2228 |
T3316 |
T3315 |
dep |
6E10,Amresco |
| R2229 |
T3317 |
T3316 |
cc |
and,6E10 |
| R2230 |
T3318 |
T3316 |
conj |
22C11,6E10 |
| R2231 |
T3319 |
T3315 |
punct |
;,Amresco |
| R2232 |
T3320 |
T3315 |
punct |
", ",Amresco |
| R2233 |
T3321 |
T3315 |
npadvmod |
Solon,Amresco |
| R2234 |
T3322 |
T3315 |
punct |
", ",Amresco |
| R2235 |
T3323 |
T3315 |
npadvmod |
Ohio,Amresco |
| R2236 |
T3324 |
T3315 |
punct |
", ",Amresco |
| R2237 |
T3325 |
T3326 |
compound |
United,States |
| R2238 |
T3326 |
T3315 |
npadvmod |
States,Amresco |
| R2239 |
T3327 |
T3315 |
punct |
),Amresco |
| R2240 |
T3328 |
T3306 |
cc |
or,buffer |
| R2241 |
T3329 |
T3330 |
nummod |
1,buffer |
| R2242 |
T3330 |
T3306 |
conj |
buffer,buffer |
| R2243 |
T3331 |
T3329 |
punct |
×,1 |
| R2244 |
T3332 |
T3333 |
compound |
Tris,SDS |
| R2245 |
T3333 |
T3330 |
compound |
SDS,buffer |
| R2246 |
T3334 |
T3333 |
punct |
-,SDS |
| R2247 |
T3335 |
T3333 |
compound |
tricine,SDS |
| R2248 |
T3336 |
T3333 |
punct |
-,SDS |
| R2249 |
T3337 |
T3338 |
punct |
(,Invitrogen |
| R2250 |
T3338 |
T3330 |
parataxis |
Invitrogen,buffer |
| R2251 |
T3339 |
T3338 |
dep |
CT15,Invitrogen |
| R2252 |
T3340 |
T3338 |
punct |
;,Invitrogen |
| R2253 |
T3341 |
T3338 |
punct |
", ",Invitrogen |
| R2254 |
T3342 |
T3338 |
npadvmod |
Carlsbad,Invitrogen |
| R2255 |
T3343 |
T3338 |
punct |
", ",Invitrogen |
| R2256 |
T3344 |
T3338 |
npadvmod |
California,Invitrogen |
| R2257 |
T3345 |
T3338 |
punct |
", ",Invitrogen |
| R2258 |
T3346 |
T3347 |
compound |
United,States |
| R2259 |
T3347 |
T3338 |
npadvmod |
States,Invitrogen |
| R2260 |
T3348 |
T3338 |
punct |
),Invitrogen |
| R2261 |
T3349 |
T3241 |
punct |
.,loaded |
| R2262 |
T3351 |
T3352 |
nsubjpass |
Proteins,transferred |
| R2263 |
T3353 |
T3352 |
auxpass |
were,transferred |
| R2264 |
T3354 |
T3352 |
advmod |
overnight,transferred |
| R2265 |
T3355 |
T3352 |
prep |
to,transferred |
| R2266 |
T3356 |
T3357 |
nummod |
0.45,μm |
| R2267 |
T3357 |
T3359 |
compound |
μm,nitrocellulose |
| R2268 |
T3358 |
T3357 |
punct |
-,μm |
| R2269 |
T3359 |
T3355 |
pobj |
nitrocellulose,to |
| R2270 |
T3360 |
T3359 |
compound |
Optitran,nitrocellulose |
| R2271 |
T3361 |
T3362 |
punct |
(,Schleicher |
| R2272 |
T3362 |
T3359 |
parataxis |
Schleicher,nitrocellulose |
| R2273 |
T3363 |
T3362 |
cc |
and,Schleicher |
| R2274 |
T3364 |
T3362 |
conj |
Schuell,Schleicher |
| R2275 |
T3365 |
T3362 |
punct |
", ",Schleicher |
| R2276 |
T3366 |
T3362 |
npadvmod |
Keene,Schleicher |
| R2277 |
T3367 |
T3362 |
punct |
", ",Schleicher |
| R2278 |
T3368 |
T3369 |
compound |
New,Hampshire |
| R2279 |
T3369 |
T3362 |
npadvmod |
Hampshire,Schleicher |
| R2280 |
T3370 |
T3362 |
punct |
", ",Schleicher |
| R2281 |
T3371 |
T3372 |
compound |
United,States |
| R2282 |
T3372 |
T3362 |
npadvmod |
States,Schleicher |
| R2283 |
T3373 |
T3362 |
punct |
),Schleicher |
| R2284 |
T3374 |
T3352 |
prep |
in,transferred |
| R2285 |
T3375 |
T3376 |
nummod |
1,buffer |
| R2286 |
T3376 |
T3374 |
pobj |
buffer,in |
| R2287 |
T3377 |
T3375 |
punct |
×,1 |
| R2288 |
T3378 |
T3376 |
compound |
TG,buffer |
| R2289 |
T3379 |
T3380 |
punct |
(,Amresco |
| R2290 |
T3380 |
T3376 |
parataxis |
Amresco,buffer |
| R2291 |
T3381 |
T3380 |
punct |
),Amresco |
| R2292 |
T3382 |
T3352 |
punct |
.,transferred |
| R2293 |
T3384 |
T3385 |
nsubjpass |
Blots,blocked |
| R2294 |
T3386 |
T3385 |
auxpass |
were,blocked |
| R2295 |
T3387 |
T3385 |
prep |
in,blocked |
| R2296 |
T3388 |
T3387 |
pobj |
PBS,in |
| R2297 |
T3389 |
T3388 |
acl |
containing,PBS |
| R2298 |
T3390 |
T3391 |
nummod |
5,% |
| R2299 |
T3391 |
T3392 |
nmod |
%,powder |
| R2300 |
T3392 |
T3389 |
dobj |
powder,containing |
| R2301 |
T3393 |
T3394 |
amod |
nonfat,milk |
| R2302 |
T3394 |
T3392 |
compound |
milk,powder |
| R2303 |
T3395 |
T3394 |
amod |
dry,milk |
| R2304 |
T3396 |
T3385 |
punct |
", ",blocked |
| R2305 |
T3397 |
T3385 |
cc |
and,blocked |
| R2306 |
T3398 |
T3385 |
conj |
incubated,blocked |
| R2307 |
T3399 |
T3398 |
prep |
for,incubated |
| R2308 |
T3400 |
T3401 |
nummod |
3,h |
| R2309 |
T3401 |
T3399 |
pobj |
h,for |
| R2310 |
T3402 |
T3398 |
prep |
at,incubated |
| R2311 |
T3403 |
T3404 |
compound |
room,temperature |
| R2312 |
T3404 |
T3402 |
pobj |
temperature,at |
| R2313 |
T3405 |
T3398 |
prep |
in,incubated |
| R2314 |
T3406 |
T3407 |
compound |
blocking,solution |
| R2315 |
T3407 |
T3405 |
pobj |
solution,in |
| R2316 |
T3408 |
T3407 |
prep |
with,solution |
| R2317 |
T3409 |
T3408 |
pobj |
one,with |
| R2318 |
T3410 |
T3409 |
prep |
of,one |
| R2319 |
T3411 |
T3412 |
det |
the,antibodies |
| R2320 |
T3412 |
T3410 |
pobj |
antibodies,of |
| R2321 |
T3413 |
T3412 |
amod |
following,antibodies |
| R2322 |
T3414 |
T3412 |
punct |
: ,antibodies |
| R2323 |
T3415 |
T3416 |
nmod |
mouse,22C11 |
| R2324 |
T3416 |
T3412 |
appos |
22C11,antibodies |
| R2325 |
T3417 |
T3416 |
amod |
monoclonal,22C11 |
| R2326 |
T3418 |
T3419 |
punct |
(,17 |
| R2327 |
T3419 |
T3416 |
parataxis |
17,22C11 |
| R2328 |
T3420 |
T3421 |
amod |
kind,gift |
| R2329 |
T3421 |
T3419 |
dep |
gift,17 |
| R2330 |
T3422 |
T3421 |
prep |
of,gift |
| R2331 |
T3423 |
T3424 |
compound |
Konrad,Beyreuther |
| R2332 |
T3424 |
T3422 |
pobj |
Beyreuther,of |
| R2333 |
T3425 |
T3424 |
cc |
and,Beyreuther |
| R2334 |
T3426 |
T3427 |
compound |
Andreas,Weidemann |
| R2335 |
T3427 |
T3424 |
conj |
Weidemann,Beyreuther |
| R2336 |
T3428 |
T3419 |
punct |
;,17 |
| R2337 |
T3429 |
T3419 |
punct |
[,17 |
| R2338 |
T3430 |
T3419 |
punct |
],17 |
| R2339 |
T3431 |
T3419 |
punct |
),17 |
| R2340 |
T3432 |
T3416 |
acl |
diluted,22C11 |
| R2341 |
T3433 |
T3432 |
npadvmod |
1,diluted |
| R2342 |
T3434 |
T3435 |
punct |
:,"1,000" |
| R2343 |
T3435 |
T3433 |
prep |
"1,000",1 |
| R2344 |
T3436 |
T3416 |
punct |
", ",22C11 |
| R2345 |
T3437 |
T3438 |
nmod |
mouse,6E10 |
| R2346 |
T3438 |
T3416 |
conj |
6E10,22C11 |
| R2347 |
T3439 |
T3438 |
amod |
monoclonal,6E10 |
| R2348 |
T3440 |
T3441 |
punct |
(,Laboratories |
| R2349 |
T3441 |
T3438 |
parataxis |
Laboratories,6E10 |
| R2350 |
T3442 |
T3441 |
compound |
Signet,Laboratories |
| R2351 |
T3443 |
T3441 |
punct |
", ",Laboratories |
| R2352 |
T3444 |
T3441 |
npadvmod |
Dedham,Laboratories |
| R2353 |
T3445 |
T3441 |
punct |
", ",Laboratories |
| R2354 |
T3446 |
T3441 |
npadvmod |
Massachusetts,Laboratories |
| R2355 |
T3447 |
T3441 |
punct |
", ",Laboratories |
| R2356 |
T3448 |
T3449 |
compound |
United,States |
| R2357 |
T3449 |
T3441 |
npadvmod |
States,Laboratories |
| R2358 |
T3450 |
T3441 |
punct |
),Laboratories |
| R2359 |
T3451 |
T3438 |
acl |
diluted,6E10 |
| R2360 |
T3452 |
T3451 |
npadvmod |
1,diluted |
| R2361 |
T3453 |
T3454 |
punct |
:,"2,500" |
| R2362 |
T3454 |
T3452 |
prep |
"2,500",1 |
| R2363 |
T3455 |
T3438 |
punct |
", ",6E10 |
| R2364 |
T3456 |
T3457 |
nmod |
rabbit,hSOD1 |
| R2365 |
T3457 |
T3438 |
conj |
hSOD1,6E10 |
| R2366 |
T3458 |
T3457 |
amod |
polyclonal,hSOD1 |
| R2367 |
T3459 |
T3457 |
amod |
anti-superoxide,hSOD1 |
| R2368 |
T3460 |
T3457 |
nmod |
dismutase,hSOD1 |
| R2369 |
T3461 |
T3457 |
nummod |
1,hSOD1 |
| R2370 |
T3462 |
T3457 |
punct |
(,hSOD1 |
| R2371 |
T3463 |
T3457 |
compound |
m,hSOD1 |
| R2372 |
T3464 |
T3457 |
punct |
/,hSOD1 |
| R2373 |
T3465 |
T3457 |
punct |
),hSOD1 |
| R2374 |
T3466 |
T3467 |
punct |
[,18 |
| R2375 |
T3467 |
T3457 |
parataxis |
18,hSOD1 |
| R2376 |
T3468 |
T3467 |
punct |
],18 |
| R2377 |
T3469 |
T3457 |
acl |
diluted,hSOD1 |
| R2378 |
T3470 |
T3469 |
npadvmod |
1,diluted |
| R2379 |
T3471 |
T3472 |
punct |
:,"2,500" |
| R2380 |
T3472 |
T3470 |
prep |
"2,500",1 |
| R2381 |
T3473 |
T3470 |
prep |
to,1 |
| R2382 |
T3474 |
T3473 |
pobj |
1,to |
| R2383 |
T3475 |
T3476 |
punct |
:,"4,000" |
| R2384 |
T3476 |
T3474 |
prep |
"4,000",1 |
| R2385 |
T3477 |
T3457 |
punct |
", ",hSOD1 |
| R2386 |
T3478 |
T3457 |
cc |
or,hSOD1 |
| R2387 |
T3479 |
T3480 |
nmod |
rabbit,CT15 |
| R2388 |
T3480 |
T3457 |
conj |
CT15,hSOD1 |
| R2389 |
T3481 |
T3480 |
amod |
polyclonal,CT15 |
| R2390 |
T3482 |
T3483 |
punct |
(,gift |
| R2391 |
T3483 |
T3480 |
parataxis |
gift,CT15 |
| R2392 |
T3484 |
T3483 |
amod |
kind,gift |
| R2393 |
T3485 |
T3483 |
prep |
of,gift |
| R2394 |
T3486 |
T3487 |
compound |
Ed,Koo |
| R2395 |
T3487 |
T3485 |
pobj |
Koo,of |
| R2396 |
T3488 |
T3483 |
punct |
;,gift |
| R2397 |
T3489 |
T3490 |
punct |
[,19 |
| R2398 |
T3490 |
T3483 |
parataxis |
19,gift |
| R2399 |
T3491 |
T3490 |
punct |
],19 |
| R2400 |
T3492 |
T3483 |
punct |
),gift |
| R2401 |
T3493 |
T3480 |
acl |
diluted,CT15 |
| R2402 |
T3494 |
T3493 |
npadvmod |
1,diluted |
| R2403 |
T3495 |
T3496 |
punct |
:,"1,000" |
| R2404 |
T3496 |
T3494 |
prep |
"1,000",1 |
| R2405 |
T3497 |
T3385 |
punct |
.,blocked |
| R2406 |
T3499 |
T3500 |
advmod |
Subsequently,washed |
| R2407 |
T3501 |
T3500 |
punct |
", ",washed |
| R2408 |
T3502 |
T3503 |
det |
the,blots |
| R2409 |
T3503 |
T3500 |
nsubjpass |
blots,washed |
| R2410 |
T3504 |
T3500 |
auxpass |
were,washed |
| R2411 |
T3505 |
T3500 |
prep |
with,washed |
| R2412 |
T3506 |
T3505 |
pobj |
PBS,with |
| R2413 |
T3507 |
T3506 |
acl |
containing,PBS |
| R2414 |
T3508 |
T3509 |
nummod |
0.1,% |
| R2415 |
T3509 |
T3510 |
compound |
%,Tween |
| R2416 |
T3510 |
T3507 |
dobj |
Tween,containing |
| R2417 |
T3511 |
T3510 |
punct |
-,Tween |
| R2418 |
T3512 |
T3510 |
nummod |
20,Tween |
| R2419 |
T3513 |
T3500 |
punct |
", ",washed |
| R2420 |
T3514 |
T3500 |
cc |
and,washed |
| R2421 |
T3515 |
T3516 |
advmod |
then,incubated |
| R2422 |
T3516 |
T3500 |
conj |
incubated,washed |
| R2423 |
T3517 |
T3516 |
prep |
with,incubated |
| R2424 |
T3518 |
T3519 |
preconj |
either,goat |
| R2425 |
T3519 |
T3520 |
nmod |
goat,antibody |
| R2426 |
T3520 |
T3517 |
pobj |
antibody,with |
| R2427 |
T3521 |
T3519 |
amod |
anti-mouse,goat |
| R2428 |
T3522 |
T3519 |
punct |
–,goat |
| R2429 |
T3523 |
T3519 |
cc |
or,goat |
| R2430 |
T3524 |
T3519 |
conj |
goat,goat |
| R2431 |
T3525 |
T3524 |
amod |
anti-rabbit,goat |
| R2432 |
T3526 |
T3520 |
punct |
–,antibody |
| R2433 |
T3527 |
T3528 |
npadvmod |
HRP,conjugated |
| R2434 |
T3528 |
T3520 |
amod |
conjugated,antibody |
| R2435 |
T3529 |
T3520 |
amod |
secondary,antibody |
| R2436 |
T3530 |
T3520 |
acl |
diluted,antibody |
| R2437 |
T3531 |
T3530 |
npadvmod |
1,diluted |
| R2438 |
T3532 |
T3533 |
punct |
:,"1,000" |
| R2439 |
T3533 |
T3531 |
prep |
"1,000",1 |
| R2440 |
T3534 |
T3530 |
prep |
in,diluted |
| R2441 |
T3535 |
T3536 |
compound |
blocking,solution |
| R2442 |
T3536 |
T3534 |
pobj |
solution,in |
| R2443 |
T3537 |
T3500 |
punct |
.,washed |
| R2444 |
T3539 |
T3540 |
prep |
After,developed |
| R2445 |
T3541 |
T3542 |
amod |
several,rinses |
| R2446 |
T3542 |
T3539 |
pobj |
rinses,After |
| R2447 |
T3543 |
T3542 |
amod |
additional,rinses |
| R2448 |
T3544 |
T3542 |
prep |
in,rinses |
| R2449 |
T3545 |
T3544 |
pobj |
PBS,in |
| R2450 |
T3546 |
T3542 |
prep |
with,rinses |
| R2451 |
T3547 |
T3548 |
nummod |
0.1,Tween |
| R2452 |
T3548 |
T3546 |
pobj |
Tween,with |
| R2453 |
T3549 |
T3547 |
quantmod |
%,0.1 |
| R2454 |
T3550 |
T3548 |
punct |
-,Tween |
| R2455 |
T3551 |
T3548 |
nummod |
20,Tween |
| R2456 |
T3552 |
T3540 |
punct |
", ",developed |
| R2457 |
T3553 |
T3540 |
nsubjpass |
blots,developed |
| R2458 |
T3554 |
T3540 |
auxpass |
were,developed |
| R2459 |
T3555 |
T3540 |
prep |
with,developed |
| R2460 |
T3556 |
T3557 |
amod |
enhanced,reagent |
| R2461 |
T3557 |
T3555 |
pobj |
reagent,with |
| R2462 |
T3558 |
T3557 |
compound |
chemiluminescence,reagent |
| R2463 |
T3559 |
T3540 |
cc |
and,developed |
| R2464 |
T3560 |
T3540 |
conj |
imaged,developed |
| R2465 |
T3561 |
T3560 |
prep |
with,imaged |
| R2466 |
T3562 |
T3563 |
det |
the,system |
| R2467 |
T3563 |
T3561 |
pobj |
system,with |
| R2468 |
T3564 |
T3565 |
compound |
Bio,Rad |
| R2469 |
T3565 |
T3567 |
compound |
Rad,FX |
| R2470 |
T3566 |
T3565 |
punct |
-,Rad |
| R2471 |
T3567 |
T3563 |
compound |
FX,system |
| R2472 |
T3568 |
T3567 |
compound |
Molecular,FX |
| R2473 |
T3569 |
T3567 |
compound |
Imager,FX |
| R2474 |
T3570 |
T3540 |
punct |
.,developed |
