Id |
Subject |
Object |
Predicate |
Lexical cue |
T3878 |
0-7 |
JJ |
denotes |
Primary |
T3880 |
8-17 |
JJ |
denotes |
embryonic |
T3879 |
18-29 |
NNS |
denotes |
fibroblasts |
T3882 |
30-34 |
VBD |
denotes |
were |
T3881 |
35-43 |
VBN |
denotes |
isolated |
T3883 |
44-48 |
IN |
denotes |
from |
T3884 |
49-50 |
DT |
denotes |
a |
T3886 |
51-55 |
CD |
denotes |
12.5 |
T3885 |
56-59 |
NN |
denotes |
day |
T3887 |
60-63 |
JJ |
denotes |
old |
T3873 |
64-72 |
NN |
denotes |
Mtf1loxP |
T3889 |
73-78 |
NN |
denotes |
mouse |
T3888 |
79-85 |
NN |
denotes |
embryo |
T3890 |
86-89 |
CC |
denotes |
and |
T3891 |
90-95 |
VBN |
denotes |
grown |
T3892 |
96-98 |
IN |
denotes |
in |
T3893 |
99-103 |
NN |
denotes |
DMEM |
T3894 |
104-116 |
VBN |
denotes |
supplemented |
T3895 |
117-121 |
IN |
denotes |
with |
T3896 |
122-124 |
CD |
denotes |
10 |
T3897 |
124-125 |
NN |
denotes |
% |
T3899 |
126-131 |
JJ |
denotes |
fetal |
T3900 |
132-138 |
JJ |
denotes |
bovine |
T3898 |
139-144 |
NN |
denotes |
serum |
T3901 |
145-146 |
-LRB- |
denotes |
( |
T3902 |
146-149 |
NN |
denotes |
ICN |
T3903 |
149-150 |
-RRB- |
denotes |
) |
T3904 |
150-152 |
, |
denotes |
, |
T3905 |
152-155 |
CD |
denotes |
100 |
T3906 |
156-157 |
NN |
denotes |
U |
T3907 |
157-158 |
SYM |
denotes |
/ |
T3908 |
158-160 |
NN |
denotes |
ml |
T3909 |
161-171 |
NN |
denotes |
penicillin |
T3910 |
171-172 |
HYPH |
denotes |
– |
T3874 |
172-184 |
NN |
denotes |
streptomycin |
T3911 |
185-186 |
-LRB- |
denotes |
( |
T3913 |
186-191 |
NNP |
denotes |
Gibco |
T3912 |
192-195 |
NNP |
denotes |
BRL |
T3914 |
195-196 |
-RRB- |
denotes |
) |
T3915 |
197-200 |
CC |
denotes |
and |
T3916 |
201-202 |
CD |
denotes |
2 |
T3917 |
203-205 |
NN |
denotes |
mM |
T3919 |
206-207 |
NN |
denotes |
l |
T3920 |
207-208 |
HYPH |
denotes |
- |
T3918 |
208-217 |
NN |
denotes |
glutamine |
T3921 |
218-219 |
-LRB- |
denotes |
( |
T3923 |
219-224 |
NNP |
denotes |
Gibco |
T3922 |
225-228 |
NNP |
denotes |
BRL |
T3924 |
228-229 |
-RRB- |
denotes |
) |
T3925 |
229-230 |
. |
denotes |
. |
T3926 |
230-500 |
sentence |
denotes |
100 mm plates with primary cells were transfected with 10 µg of an expression plasmid coding for simian virus 40 (SV40) large T antigen driven by the cytomegalovirus (CMV) promoter, using lipofectamine™ reagent (Invitrogen) according to the manufacturer's instructions. |
T3927 |
231-234 |
CD |
denotes |
100 |
T3928 |
235-237 |
NN |
denotes |
mm |
T3929 |
238-244 |
NNS |
denotes |
plates |
T3931 |
245-249 |
IN |
denotes |
with |
T3932 |
250-257 |
JJ |
denotes |
primary |
T3933 |
258-263 |
NNS |
denotes |
cells |
T3934 |
264-268 |
VBD |
denotes |
were |
T3930 |
269-280 |
VBN |
denotes |
transfected |
T3935 |
281-285 |
IN |
denotes |
with |
T3936 |
286-288 |
CD |
denotes |
10 |
T3937 |
289-291 |
NN |
denotes |
µg |
T3938 |
292-294 |
IN |
denotes |
of |
T3939 |
295-297 |
DT |
denotes |
an |
T3941 |
298-308 |
NN |
denotes |
expression |
T3940 |
309-316 |
NN |
denotes |
plasmid |
T3942 |
317-323 |
VBG |
denotes |
coding |
T3943 |
324-327 |
IN |
denotes |
for |
T3944 |
328-334 |
JJ |
denotes |
simian |
T3945 |
335-340 |
NN |
denotes |
virus |
T3947 |
341-343 |
CD |
denotes |
40 |
T3948 |
344-345 |
-LRB- |
denotes |
( |
T3949 |
345-349 |
NN |
denotes |
SV40 |
T3950 |
349-350 |
-RRB- |
denotes |
) |
T3951 |
351-356 |
JJ |
denotes |
large |
T3952 |
357-358 |
NN |
denotes |
T |
T3946 |
359-366 |
NN |
denotes |
antigen |
T3953 |
367-373 |
VBN |
denotes |
driven |
T3954 |
374-376 |
IN |
denotes |
by |
T3955 |
377-380 |
DT |
denotes |
the |
T3957 |
381-396 |
NN |
denotes |
cytomegalovirus |
T3958 |
397-398 |
-LRB- |
denotes |
( |
T3959 |
398-401 |
NN |
denotes |
CMV |
T3960 |
401-402 |
-RRB- |
denotes |
) |
T3956 |
403-411 |
NN |
denotes |
promoter |
T3961 |
411-413 |
, |
denotes |
, |
T3962 |
413-418 |
VBG |
denotes |
using |
T3963 |
419-432 |
NNP |
denotes |
lipofectamine |
T3965 |
432-433 |
SYM |
denotes |
™ |
T3964 |
434-441 |
NN |
denotes |
reagent |
T3966 |
442-443 |
-LRB- |
denotes |
( |
T3967 |
443-453 |
NNP |
denotes |
Invitrogen |
T3968 |
453-454 |
-RRB- |
denotes |
) |
T3969 |
455-464 |
VBG |
denotes |
according |
T3970 |
465-467 |
IN |
denotes |
to |
T3971 |
468-471 |
DT |
denotes |
the |
T3972 |
472-484 |
NN |
denotes |
manufacturer |
T3974 |
484-486 |
POS |
denotes |
's |
T3973 |
487-499 |
NNS |
denotes |
instructions |
T3975 |
499-500 |
. |
denotes |
. |
T3976 |
500-617 |
sentence |
denotes |
Cell foci were isolated and the immortalized mouse embryonic fibroblast cell line ckoC was derived from one of them. |
T3977 |
501-505 |
NN |
denotes |
Cell |
T3978 |
506-510 |
NNS |
denotes |
foci |
T3980 |
511-515 |
VBD |
denotes |
were |
T3979 |
516-524 |
VBN |
denotes |
isolated |
T3981 |
525-528 |
CC |
denotes |
and |
T3982 |
529-532 |
DT |
denotes |
the |
T3984 |
533-545 |
VBN |
denotes |
immortalized |
T3985 |
546-551 |
NN |
denotes |
mouse |
T3987 |
552-561 |
JJ |
denotes |
embryonic |
T3986 |
562-572 |
NN |
denotes |
fibroblast |
T3988 |
573-577 |
NN |
denotes |
cell |
T3983 |
578-582 |
NN |
denotes |
line |
T3990 |
583-587 |
NN |
denotes |
ckoC |
T3991 |
588-591 |
VBD |
denotes |
was |
T3989 |
592-599 |
VBN |
denotes |
derived |
T3992 |
600-604 |
IN |
denotes |
from |
T3993 |
605-608 |
CD |
denotes |
one |
T3994 |
609-611 |
IN |
denotes |
of |
T3995 |
612-616 |
PRP |
denotes |
them |
T3996 |
616-617 |
. |
denotes |
. |
T3997 |
617-727 |
sentence |
denotes |
The Mtf1loxP genotype of this line as well as the genomic integration of the T antigen were confirmed by PCR. |
T3998 |
618-621 |
DT |
denotes |
The |
T4000 |
622-630 |
NN |
denotes |
Mtf1loxP |
T3999 |
631-639 |
NN |
denotes |
genotype |
T4002 |
640-642 |
IN |
denotes |
of |
T4003 |
643-647 |
DT |
denotes |
this |
T4004 |
648-652 |
NN |
denotes |
line |
T4005 |
653-655 |
RB |
denotes |
as |
T4007 |
656-660 |
RB |
denotes |
well |
T4006 |
661-663 |
IN |
denotes |
as |
T4008 |
664-667 |
DT |
denotes |
the |
T4010 |
668-675 |
JJ |
denotes |
genomic |
T4009 |
676-687 |
NN |
denotes |
integration |
T4011 |
688-690 |
IN |
denotes |
of |
T4012 |
691-694 |
DT |
denotes |
the |
T4014 |
695-696 |
NN |
denotes |
T |
T4013 |
697-704 |
NN |
denotes |
antigen |
T4015 |
705-709 |
VBD |
denotes |
were |
T4001 |
710-719 |
VBN |
denotes |
confirmed |
T4016 |
720-722 |
IN |
denotes |
by |
T4017 |
723-726 |
NN |
denotes |
PCR |
T4018 |
726-727 |
. |
denotes |
. |
T4019 |
727-1012 |
sentence |
denotes |
100 mm plates with these cells were further transfected by the calcium phosphate method (30) with 19.6 µg of an expression plasmid for Cre recombinase driven by the CMV promoter and 0.4 µg of an expression plasmid for the neomycin resistance gene under the control of the TK promoter. |
T4020 |
728-731 |
CD |
denotes |
100 |
T4021 |
732-734 |
NN |
denotes |
mm |
T4022 |
735-741 |
NNS |
denotes |
plates |
T4024 |
742-746 |
IN |
denotes |
with |
T4025 |
747-752 |
DT |
denotes |
these |
T4026 |
753-758 |
NNS |
denotes |
cells |
T4027 |
759-763 |
VBD |
denotes |
were |
T4028 |
764-771 |
RB |
denotes |
further |
T4023 |
772-783 |
VBN |
denotes |
transfected |
T4029 |
784-786 |
IN |
denotes |
by |
T4030 |
787-790 |
DT |
denotes |
the |
T4032 |
791-798 |
NN |
denotes |
calcium |
T4033 |
799-808 |
NN |
denotes |
phosphate |
T4031 |
809-815 |
NN |
denotes |
method |
T4034 |
816-817 |
-LRB- |
denotes |
( |
T4035 |
817-819 |
CD |
denotes |
30 |
T4036 |
819-820 |
-RRB- |
denotes |
) |
T4037 |
821-825 |
IN |
denotes |
with |
T4038 |
826-830 |
CD |
denotes |
19.6 |
T4039 |
831-833 |
NN |
denotes |
µg |
T4040 |
834-836 |
IN |
denotes |
of |
T4041 |
837-839 |
DT |
denotes |
an |
T4043 |
840-850 |
NN |
denotes |
expression |
T4042 |
851-858 |
NN |
denotes |
plasmid |
T4044 |
859-862 |
IN |
denotes |
for |
T4045 |
863-866 |
NN |
denotes |
Cre |
T4046 |
867-878 |
NN |
denotes |
recombinase |
T4047 |
879-885 |
VBN |
denotes |
driven |
T4048 |
886-888 |
IN |
denotes |
by |
T4049 |
889-892 |
DT |
denotes |
the |
T4051 |
893-896 |
NN |
denotes |
CMV |
T4050 |
897-905 |
NN |
denotes |
promoter |
T4052 |
906-909 |
CC |
denotes |
and |
T4053 |
910-913 |
CD |
denotes |
0.4 |
T4054 |
914-916 |
NN |
denotes |
µg |
T4055 |
917-919 |
IN |
denotes |
of |
T4056 |
920-922 |
DT |
denotes |
an |
T4058 |
923-933 |
NN |
denotes |
expression |
T4057 |
934-941 |
NN |
denotes |
plasmid |
T4059 |
942-945 |
IN |
denotes |
for |
T4060 |
946-949 |
DT |
denotes |
the |
T4062 |
950-958 |
NN |
denotes |
neomycin |
T4063 |
959-969 |
NN |
denotes |
resistance |
T4061 |
970-974 |
NN |
denotes |
gene |
T4064 |
975-980 |
IN |
denotes |
under |
T4065 |
981-984 |
DT |
denotes |
the |
T4066 |
985-992 |
NN |
denotes |
control |
T4067 |
993-995 |
IN |
denotes |
of |
T4068 |
996-999 |
DT |
denotes |
the |
T4070 |
1000-1002 |
NN |
denotes |
TK |
T4069 |
1003-1011 |
NN |
denotes |
promoter |
T4071 |
1011-1012 |
. |
denotes |
. |
T4072 |
1012-1275 |
sentence |
denotes |
Stably transfected cells were selected in the presence of 0.4 µg/µl G418 (Calbiochem), isolated clones of resistant cells were harvested and grown independently, and the expression of Cre recombinase and excision of exons 3 and 4 of Mtf1 were analyzed by RT–PCR. |
T4073 |
1013-1019 |
RB |
denotes |
Stably |
T4074 |
1020-1031 |
VBN |
denotes |
transfected |
T4075 |
1032-1037 |
NNS |
denotes |
cells |
T4077 |
1038-1042 |
VBD |
denotes |
were |
T4076 |
1043-1051 |
VBN |
denotes |
selected |
T4078 |
1052-1054 |
IN |
denotes |
in |
T4079 |
1055-1058 |
DT |
denotes |
the |
T4080 |
1059-1067 |
NN |
denotes |
presence |
T4081 |
1068-1070 |
IN |
denotes |
of |
T4082 |
1071-1074 |
CD |
denotes |
0.4 |
T4083 |
1075-1077 |
NN |
denotes |
µg |
T4085 |
1077-1078 |
SYM |
denotes |
/ |
T4086 |
1078-1080 |
NN |
denotes |
µl |
T4084 |
1081-1085 |
NN |
denotes |
G418 |
T4087 |
1086-1087 |
-LRB- |
denotes |
( |
T4088 |
1087-1097 |
NNP |
denotes |
Calbiochem |
T4089 |
1097-1098 |
-RRB- |
denotes |
) |
T4090 |
1098-1100 |
, |
denotes |
, |
T4091 |
1100-1108 |
VBN |
denotes |
isolated |
T4092 |
1109-1115 |
NNS |
denotes |
clones |
T4094 |
1116-1118 |
IN |
denotes |
of |
T4095 |
1119-1128 |
JJ |
denotes |
resistant |
T4096 |
1129-1134 |
NNS |
denotes |
cells |
T4097 |
1135-1139 |
VBD |
denotes |
were |
T4093 |
1140-1149 |
VBN |
denotes |
harvested |
T4098 |
1150-1153 |
CC |
denotes |
and |
T4099 |
1154-1159 |
VBN |
denotes |
grown |
T4100 |
1160-1173 |
RB |
denotes |
independently |
T4101 |
1173-1175 |
, |
denotes |
, |
T4102 |
1175-1178 |
CC |
denotes |
and |
T4103 |
1179-1182 |
DT |
denotes |
the |
T4104 |
1183-1193 |
NN |
denotes |
expression |
T4106 |
1194-1196 |
IN |
denotes |
of |
T4107 |
1197-1200 |
NN |
denotes |
Cre |
T4108 |
1201-1212 |
NN |
denotes |
recombinase |
T4109 |
1213-1216 |
CC |
denotes |
and |
T4110 |
1217-1225 |
NN |
denotes |
excision |
T4111 |
1226-1228 |
IN |
denotes |
of |
T4112 |
1229-1234 |
NNS |
denotes |
exons |
T4113 |
1235-1236 |
CD |
denotes |
3 |
T4114 |
1237-1240 |
CC |
denotes |
and |
T4115 |
1241-1242 |
CD |
denotes |
4 |
T4116 |
1243-1245 |
IN |
denotes |
of |
T4117 |
1246-1250 |
NN |
denotes |
Mtf1 |
T4118 |
1251-1255 |
VBD |
denotes |
were |
T4105 |
1256-1264 |
VBN |
denotes |
analyzed |
T4119 |
1265-1267 |
IN |
denotes |
by |
T4120 |
1268-1270 |
NN |
denotes |
RT |
T4122 |
1270-1271 |
HYPH |
denotes |
– |
T4121 |
1271-1274 |
NN |
denotes |
PCR |
T4123 |
1274-1275 |
. |
denotes |
. |
T4124 |
1275-1377 |
sentence |
denotes |
The cell lines delC19, delC21 and delC23 with a deletion of Mtf1 were chosen for further experiments. |
T4125 |
1276-1279 |
DT |
denotes |
The |
T4127 |
1280-1284 |
NN |
denotes |
cell |
T4126 |
1285-1290 |
NNS |
denotes |
lines |
T4129 |
1291-1297 |
NN |
denotes |
delC19 |
T4130 |
1297-1299 |
, |
denotes |
, |
T4131 |
1299-1305 |
NN |
denotes |
delC21 |
T4132 |
1306-1309 |
CC |
denotes |
and |
T4133 |
1310-1316 |
NN |
denotes |
delC23 |
T4134 |
1317-1321 |
IN |
denotes |
with |
T4135 |
1322-1323 |
DT |
denotes |
a |
T4136 |
1324-1332 |
NN |
denotes |
deletion |
T4137 |
1333-1335 |
IN |
denotes |
of |
T4138 |
1336-1340 |
NN |
denotes |
Mtf1 |
T4139 |
1341-1345 |
VBD |
denotes |
were |
T4128 |
1346-1352 |
VBN |
denotes |
chosen |
T4140 |
1353-1356 |
IN |
denotes |
for |
T4141 |
1357-1364 |
JJ |
denotes |
further |
T4142 |
1365-1376 |
NNS |
denotes |
experiments |
T4143 |
1376-1377 |
. |
denotes |
. |
R2537 |
T3873 |
T3888 |
compound |
Mtf1loxP,embryo |
R2538 |
T3874 |
T3898 |
conj |
streptomycin,serum |
R2540 |
T3878 |
T3879 |
amod |
Primary,fibroblasts |
R2541 |
T3879 |
T3881 |
nsubjpass |
fibroblasts,isolated |
R2542 |
T3880 |
T3879 |
amod |
embryonic,fibroblasts |
R2543 |
T3882 |
T3881 |
auxpass |
were,isolated |
R2544 |
T3883 |
T3881 |
prep |
from,isolated |
R2545 |
T3884 |
T3885 |
det |
a,day |
R2546 |
T3885 |
T3887 |
npadvmod |
day,old |
R2547 |
T3886 |
T3885 |
nummod |
12.5,day |
R2548 |
T3887 |
T3888 |
amod |
old,embryo |
R2549 |
T3888 |
T3883 |
pobj |
embryo,from |
R2550 |
T3889 |
T3888 |
compound |
mouse,embryo |
R2551 |
T3890 |
T3881 |
cc |
and,isolated |
R2552 |
T3891 |
T3881 |
conj |
grown,isolated |
R2553 |
T3892 |
T3891 |
prep |
in,grown |
R2554 |
T3893 |
T3892 |
pobj |
DMEM,in |
R2555 |
T3894 |
T3893 |
acl |
supplemented,DMEM |
R2556 |
T3895 |
T3894 |
prep |
with,supplemented |
R2557 |
T3896 |
T3897 |
nummod |
10,% |
R2558 |
T3897 |
T3898 |
nmod |
%,serum |
R2559 |
T3898 |
T3895 |
pobj |
serum,with |
R2560 |
T3899 |
T3898 |
amod |
fetal,serum |
R2561 |
T3900 |
T3898 |
amod |
bovine,serum |
R2562 |
T3901 |
T3902 |
punct |
(,ICN |
R2563 |
T3902 |
T3898 |
parataxis |
ICN,serum |
R2564 |
T3903 |
T3902 |
punct |
),ICN |
R2565 |
T3904 |
T3898 |
punct |
", ",serum |
R2566 |
T3905 |
T3906 |
nummod |
100,U |
R2567 |
T3906 |
T3874 |
nmod |
U,streptomycin |
R2568 |
T3907 |
T3908 |
punct |
/,ml |
R2569 |
T3908 |
T3906 |
prep |
ml,U |
R2570 |
T3909 |
T3874 |
compound |
penicillin,streptomycin |
R2571 |
T3910 |
T3874 |
punct |
–,streptomycin |
R2572 |
T3911 |
T3912 |
punct |
(,BRL |
R2573 |
T3912 |
T3874 |
parataxis |
BRL,streptomycin |
R2574 |
T3913 |
T3912 |
compound |
Gibco,BRL |
R2575 |
T3914 |
T3912 |
punct |
),BRL |
R2576 |
T3915 |
T3874 |
cc |
and,streptomycin |
R2577 |
T3916 |
T3917 |
nummod |
2,mM |
R2578 |
T3917 |
T3918 |
compound |
mM,glutamine |
R2579 |
T3918 |
T3874 |
conj |
glutamine,streptomycin |
R2580 |
T3919 |
T3918 |
compound |
l,glutamine |
R2581 |
T3920 |
T3918 |
punct |
-,glutamine |
R2582 |
T3921 |
T3922 |
punct |
(,BRL |
R2583 |
T3922 |
T3918 |
parataxis |
BRL,glutamine |
R2584 |
T3923 |
T3922 |
compound |
Gibco,BRL |
R2585 |
T3924 |
T3922 |
punct |
),BRL |
R2586 |
T3925 |
T3881 |
punct |
.,isolated |
R2587 |
T3927 |
T3928 |
nummod |
100,mm |
R2588 |
T3928 |
T3929 |
compound |
mm,plates |
R2589 |
T3929 |
T3930 |
nsubjpass |
plates,transfected |
R2590 |
T3931 |
T3929 |
prep |
with,plates |
R2591 |
T3932 |
T3933 |
amod |
primary,cells |
R2592 |
T3933 |
T3931 |
pobj |
cells,with |
R2593 |
T3934 |
T3930 |
auxpass |
were,transfected |
R2594 |
T3935 |
T3930 |
prep |
with,transfected |
R2595 |
T3936 |
T3937 |
nummod |
10,µg |
R2596 |
T3937 |
T3935 |
pobj |
µg,with |
R2597 |
T3938 |
T3937 |
prep |
of,µg |
R2598 |
T3939 |
T3940 |
det |
an,plasmid |
R2599 |
T3940 |
T3938 |
pobj |
plasmid,of |
R2600 |
T3941 |
T3940 |
compound |
expression,plasmid |
R2601 |
T3942 |
T3940 |
acl |
coding,plasmid |
R2602 |
T3943 |
T3942 |
prep |
for,coding |
R2603 |
T3944 |
T3945 |
amod |
simian,virus |
R2604 |
T3945 |
T3946 |
nmod |
virus,antigen |
R2605 |
T3946 |
T3943 |
pobj |
antigen,for |
R2606 |
T3947 |
T3945 |
nummod |
40,virus |
R2607 |
T3948 |
T3945 |
punct |
(,virus |
R2608 |
T3949 |
T3945 |
appos |
SV40,virus |
R2609 |
T3950 |
T3946 |
punct |
),antigen |
R2610 |
T3951 |
T3952 |
amod |
large,T |
R2611 |
T3952 |
T3946 |
compound |
T,antigen |
R2612 |
T3953 |
T3946 |
acl |
driven,antigen |
R2613 |
T3954 |
T3953 |
agent |
by,driven |
R2614 |
T3955 |
T3956 |
det |
the,promoter |
R2615 |
T3956 |
T3954 |
pobj |
promoter,by |
R2616 |
T3957 |
T3956 |
nmod |
cytomegalovirus,promoter |
R2617 |
T3958 |
T3957 |
punct |
(,cytomegalovirus |
R2618 |
T3959 |
T3957 |
appos |
CMV,cytomegalovirus |
R2619 |
T3960 |
T3956 |
punct |
),promoter |
R2620 |
T3961 |
T3930 |
punct |
", ",transfected |
R2621 |
T3962 |
T3930 |
advcl |
using,transfected |
R2622 |
T3963 |
T3964 |
nmod |
lipofectamine,reagent |
R2623 |
T3964 |
T3962 |
dobj |
reagent,using |
R2624 |
T3965 |
T3963 |
punct |
™,lipofectamine |
R2625 |
T3966 |
T3967 |
punct |
(,Invitrogen |
R2626 |
T3967 |
T3964 |
parataxis |
Invitrogen,reagent |
R2627 |
T3968 |
T3967 |
punct |
),Invitrogen |
R2628 |
T3969 |
T3962 |
prep |
according,using |
R2629 |
T3970 |
T3969 |
prep |
to,according |
R2630 |
T3971 |
T3972 |
det |
the,manufacturer |
R2631 |
T3972 |
T3973 |
poss |
manufacturer,instructions |
R2632 |
T3973 |
T3970 |
pobj |
instructions,to |
R2633 |
T3974 |
T3972 |
case |
's,manufacturer |
R2634 |
T3975 |
T3930 |
punct |
.,transfected |
R2635 |
T3977 |
T3978 |
compound |
Cell,foci |
R2636 |
T3978 |
T3979 |
nsubjpass |
foci,isolated |
R2637 |
T3980 |
T3979 |
auxpass |
were,isolated |
R2638 |
T3981 |
T3979 |
cc |
and,isolated |
R2639 |
T3982 |
T3983 |
det |
the,line |
R2640 |
T3983 |
T3989 |
nsubjpass |
line,derived |
R2641 |
T3984 |
T3983 |
amod |
immortalized,line |
R2642 |
T3985 |
T3986 |
nmod |
mouse,fibroblast |
R2643 |
T3986 |
T3983 |
compound |
fibroblast,line |
R2644 |
T3987 |
T3986 |
amod |
embryonic,fibroblast |
R2645 |
T3988 |
T3983 |
compound |
cell,line |
R2646 |
T3989 |
T3979 |
conj |
derived,isolated |
R2647 |
T3990 |
T3983 |
appos |
ckoC,line |
R2648 |
T3991 |
T3989 |
auxpass |
was,derived |
R2649 |
T3992 |
T3989 |
prep |
from,derived |
R2650 |
T3993 |
T3992 |
pobj |
one,from |
R2651 |
T3994 |
T3993 |
prep |
of,one |
R2652 |
T3995 |
T3994 |
pobj |
them,of |
R2653 |
T3996 |
T3989 |
punct |
.,derived |
R2654 |
T3998 |
T3999 |
det |
The,genotype |
R2655 |
T3999 |
T4001 |
nsubjpass |
genotype,confirmed |
R2656 |
T4000 |
T3999 |
compound |
Mtf1loxP,genotype |
R2657 |
T4002 |
T3999 |
prep |
of,genotype |
R2658 |
T4003 |
T4004 |
det |
this,line |
R2659 |
T4004 |
T4002 |
pobj |
line,of |
R2660 |
T4005 |
T4006 |
advmod |
as,as |
R2661 |
T4006 |
T3999 |
cc |
as,genotype |
R2662 |
T4007 |
T4006 |
advmod |
well,as |
R2663 |
T4008 |
T4009 |
det |
the,integration |
R2664 |
T4009 |
T3999 |
conj |
integration,genotype |
R2665 |
T4010 |
T4009 |
amod |
genomic,integration |
R2666 |
T4011 |
T4009 |
prep |
of,integration |
R2667 |
T4012 |
T4013 |
det |
the,antigen |
R2668 |
T4013 |
T4011 |
pobj |
antigen,of |
R2669 |
T4014 |
T4013 |
compound |
T,antigen |
R2670 |
T4015 |
T4001 |
auxpass |
were,confirmed |
R2671 |
T4016 |
T4001 |
agent |
by,confirmed |
R2672 |
T4017 |
T4016 |
pobj |
PCR,by |
R2673 |
T4018 |
T4001 |
punct |
.,confirmed |
R2674 |
T4020 |
T4021 |
nummod |
100,mm |
R2675 |
T4021 |
T4022 |
compound |
mm,plates |
R2676 |
T4022 |
T4023 |
nsubjpass |
plates,transfected |
R2677 |
T4024 |
T4022 |
prep |
with,plates |
R2678 |
T4025 |
T4026 |
det |
these,cells |
R2679 |
T4026 |
T4024 |
pobj |
cells,with |
R2680 |
T4027 |
T4023 |
auxpass |
were,transfected |
R2681 |
T4028 |
T4023 |
advmod |
further,transfected |
R2682 |
T4029 |
T4023 |
agent |
by,transfected |
R2683 |
T4030 |
T4031 |
det |
the,method |
R2684 |
T4031 |
T4029 |
pobj |
method,by |
R2685 |
T4032 |
T4033 |
compound |
calcium,phosphate |
R2686 |
T4033 |
T4031 |
compound |
phosphate,method |
R2687 |
T4034 |
T4035 |
punct |
(,30 |
R2688 |
T4035 |
T4031 |
parataxis |
30,method |
R2689 |
T4036 |
T4035 |
punct |
),30 |
R2690 |
T4037 |
T4031 |
prep |
with,method |
R2691 |
T4038 |
T4039 |
nummod |
19.6,µg |
R2692 |
T4039 |
T4037 |
pobj |
µg,with |
R2693 |
T4040 |
T4039 |
prep |
of,µg |
R2694 |
T4041 |
T4042 |
det |
an,plasmid |
R2695 |
T4042 |
T4040 |
pobj |
plasmid,of |
R2696 |
T4043 |
T4042 |
compound |
expression,plasmid |
R2697 |
T4044 |
T4042 |
prep |
for,plasmid |
R2698 |
T4045 |
T4046 |
compound |
Cre,recombinase |
R2699 |
T4046 |
T4044 |
pobj |
recombinase,for |
R2700 |
T4047 |
T4042 |
acl |
driven,plasmid |
R2701 |
T4048 |
T4047 |
agent |
by,driven |
R2702 |
T4049 |
T4050 |
det |
the,promoter |
R2703 |
T4050 |
T4048 |
pobj |
promoter,by |
R2704 |
T4051 |
T4050 |
compound |
CMV,promoter |
R2705 |
T4052 |
T4039 |
cc |
and,µg |
R2706 |
T4053 |
T4054 |
nummod |
0.4,µg |
R2707 |
T4054 |
T4039 |
conj |
µg,µg |
R2708 |
T4055 |
T4054 |
prep |
of,µg |
R2709 |
T4056 |
T4057 |
det |
an,plasmid |
R2710 |
T4057 |
T4055 |
pobj |
plasmid,of |
R2711 |
T4058 |
T4057 |
compound |
expression,plasmid |
R2712 |
T4059 |
T4057 |
prep |
for,plasmid |
R2713 |
T4060 |
T4061 |
det |
the,gene |
R2714 |
T4061 |
T4059 |
pobj |
gene,for |
R2715 |
T4062 |
T4061 |
compound |
neomycin,gene |
R2716 |
T4063 |
T4061 |
compound |
resistance,gene |
R2717 |
T4064 |
T4061 |
prep |
under,gene |
R2718 |
T4065 |
T4066 |
det |
the,control |
R2719 |
T4066 |
T4064 |
pobj |
control,under |
R2720 |
T4067 |
T4066 |
prep |
of,control |
R2721 |
T4068 |
T4069 |
det |
the,promoter |
R2722 |
T4069 |
T4067 |
pobj |
promoter,of |
R2723 |
T4070 |
T4069 |
compound |
TK,promoter |
R2724 |
T4071 |
T4023 |
punct |
.,transfected |
R2725 |
T4073 |
T4074 |
advmod |
Stably,transfected |
R2726 |
T4074 |
T4075 |
amod |
transfected,cells |
R2727 |
T4075 |
T4076 |
nsubjpass |
cells,selected |
R2728 |
T4077 |
T4076 |
auxpass |
were,selected |
R2729 |
T4078 |
T4076 |
prep |
in,selected |
R2730 |
T4079 |
T4080 |
det |
the,presence |
R2731 |
T4080 |
T4078 |
pobj |
presence,in |
R2732 |
T4081 |
T4080 |
prep |
of,presence |
R2733 |
T4082 |
T4083 |
nummod |
0.4,µg |
R2734 |
T4083 |
T4084 |
nmod |
µg,G418 |
R2735 |
T4084 |
T4081 |
pobj |
G418,of |
R2736 |
T4085 |
T4086 |
punct |
/,µl |
R2737 |
T4086 |
T4083 |
prep |
µl,µg |
R2738 |
T4087 |
T4088 |
punct |
(,Calbiochem |
R2739 |
T4088 |
T4084 |
parataxis |
Calbiochem,G418 |
R2740 |
T4089 |
T4088 |
punct |
),Calbiochem |
R2741 |
T4090 |
T4076 |
punct |
", ",selected |
R2742 |
T4091 |
T4092 |
amod |
isolated,clones |
R2743 |
T4092 |
T4093 |
nsubjpass |
clones,harvested |
R2744 |
T4093 |
T4076 |
conj |
harvested,selected |
R2745 |
T4094 |
T4092 |
prep |
of,clones |
R2746 |
T4095 |
T4096 |
amod |
resistant,cells |
R2747 |
T4096 |
T4094 |
pobj |
cells,of |
R2748 |
T4097 |
T4093 |
auxpass |
were,harvested |
R2749 |
T4098 |
T4093 |
cc |
and,harvested |
R2750 |
T4099 |
T4093 |
conj |
grown,harvested |
R2751 |
T4100 |
T4099 |
advmod |
independently,grown |
R2752 |
T4101 |
T4093 |
punct |
", ",harvested |
R2753 |
T4102 |
T4093 |
cc |
and,harvested |
R2754 |
T4103 |
T4104 |
det |
the,expression |
R2755 |
T4104 |
T4105 |
nsubjpass |
expression,analyzed |
R2756 |
T4105 |
T4093 |
conj |
analyzed,harvested |
R2757 |
T4106 |
T4104 |
prep |
of,expression |
R2758 |
T4107 |
T4108 |
compound |
Cre,recombinase |
R2759 |
T4108 |
T4106 |
pobj |
recombinase,of |
R2760 |
T4109 |
T4104 |
cc |
and,expression |
R2761 |
T4110 |
T4104 |
conj |
excision,expression |
R2762 |
T4111 |
T4110 |
prep |
of,excision |
R2763 |
T4112 |
T4113 |
nmod |
exons,3 |
R2764 |
T4113 |
T4111 |
pobj |
3,of |
R2765 |
T4114 |
T4113 |
cc |
and,3 |
R2766 |
T4115 |
T4113 |
conj |
4,3 |
R2767 |
T4116 |
T4113 |
prep |
of,3 |
R2768 |
T4117 |
T4116 |
pobj |
Mtf1,of |
R2769 |
T4118 |
T4105 |
auxpass |
were,analyzed |
R2770 |
T4119 |
T4105 |
agent |
by,analyzed |
R2771 |
T4120 |
T4121 |
compound |
RT,PCR |
R2772 |
T4121 |
T4119 |
pobj |
PCR,by |
R2773 |
T4122 |
T4121 |
punct |
–,PCR |
R2774 |
T4123 |
T4105 |
punct |
.,analyzed |
R2775 |
T4125 |
T4126 |
det |
The,lines |
R2776 |
T4126 |
T4128 |
nsubjpass |
lines,chosen |
R2777 |
T4127 |
T4126 |
compound |
cell,lines |
R2778 |
T4129 |
T4126 |
appos |
delC19,lines |
R2779 |
T4130 |
T4129 |
punct |
", ",delC19 |
R2780 |
T4131 |
T4129 |
conj |
delC21,delC19 |
R2781 |
T4132 |
T4131 |
cc |
and,delC21 |
R2782 |
T4133 |
T4131 |
conj |
delC23,delC21 |
R2783 |
T4134 |
T4126 |
prep |
with,lines |
R2784 |
T4135 |
T4136 |
det |
a,deletion |
R2785 |
T4136 |
T4134 |
pobj |
deletion,with |
R2786 |
T4137 |
T4136 |
prep |
of,deletion |
R2787 |
T4138 |
T4137 |
pobj |
Mtf1,of |
R2788 |
T4139 |
T4128 |
auxpass |
were,chosen |
R2789 |
T4140 |
T4128 |
prep |
for,chosen |
R2790 |
T4141 |
T4142 |
amod |
further,experiments |
R2791 |
T4142 |
T4140 |
pobj |
experiments,for |
R2792 |
T4143 |
T4128 |
punct |
.,chosen |