PMC:1253828 JSON TXT 10 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T239	0-3	CD	denotes	Two
T241	0-95	sentence	denotes	Two major branches of anti-cadmium defense in the mouse: MTF-1/metallothioneins and glutathione
T242	4-9	JJ	denotes	major
T240	10-18	NNS	denotes	branches
T243	19-21	IN	denotes	of
T244	22-34	JJ	denotes	anti-cadmium
T245	35-42	NN	denotes	defense
T246	43-45	IN	denotes	in
T247	46-49	DT	denotes	the
T248	50-55	NN	denotes	mouse
T249	55-57	:	denotes	:
T250	57-60	NN	denotes	MTF
T252	60-61	HYPH	denotes	-
T253	61-62	CD	denotes	1
T254	62-63	HYPH	denotes	/
T251	63-79	NNS	denotes	metallothioneins
T255	80-83	CC	denotes	and
T256	84-95	NN	denotes	glutathione
T257	95-96	sentence	denotes
T259	106-111	NN	denotes	Metal
T258	106-353	sentence	denotes	Metal-responsive transcription factor 1 (MTF-1) regulates expression of its target genes in response to various stress conditions, notably heavy metal load, via binding to metal response elements (MREs) in the respective enhancer/promoter regions.
T261	111-112	HYPH	denotes	-
T260	112-122	JJ	denotes	responsive
T263	123-136	NN	denotes	transcription
T262	137-143	NN	denotes	factor
T265	144-145	CD	denotes	1
T266	146-147	-LRB-	denotes	(
T267	147-150	NN	denotes	MTF
T268	150-151	HYPH	denotes	-
T269	151-152	CD	denotes	1
T270	152-153	-RRB-	denotes	)
T264	154-163	VBZ	denotes	regulates
T271	164-174	NN	denotes	expression
T272	175-177	IN	denotes	of
T273	178-181	PRP$	denotes	its
T275	182-188	NN	denotes	target
T274	189-194	NNS	denotes	genes
T276	195-197	IN	denotes	in
T277	198-206	NN	denotes	response
T278	207-209	IN	denotes	to
T279	210-217	JJ	denotes	various
T281	218-224	NN	denotes	stress
T280	225-235	NNS	denotes	conditions
T282	235-237	,	denotes	,
T283	237-244	RB	denotes	notably
T285	245-250	JJ	denotes	heavy
T286	251-256	NN	denotes	metal
T284	257-261	NN	denotes	load
T287	261-263	,	denotes	,
T288	263-266	IN	denotes	via
T289	267-274	NN	denotes	binding
T290	275-277	IN	denotes	to
T291	278-283	NN	denotes	metal
T293	284-292	NN	denotes	response
T292	293-301	NNS	denotes	elements
T294	302-303	-LRB-	denotes	(
T295	303-307	NNS	denotes	MREs
T296	307-308	-RRB-	denotes	)
T297	309-311	IN	denotes	in
T298	312-315	DT	denotes	the
T300	316-326	JJ	denotes	respective
T301	327-335	NN	denotes	enhancer
T303	335-336	HYPH	denotes	/
T302	336-344	NN	denotes	promoter
T299	345-352	NNS	denotes	regions
T304	352-353	.	denotes	.
T305	353-425	sentence	denotes	Furthermore, it serves a vital function in embryonic liver development.
T306	354-365	RB	denotes	Furthermore
T308	365-367	,	denotes	,
T309	367-369	PRP	denotes	it
T307	370-376	VBZ	denotes	serves
T310	377-378	DT	denotes	a
T312	379-384	JJ	denotes	vital
T311	385-393	NN	denotes	function
T313	394-396	IN	denotes	in
T314	397-406	JJ	denotes	embryonic
T316	407-412	NN	denotes	liver
T315	413-424	NN	denotes	development
T317	424-425	.	denotes	.
T318	425-506	sentence	denotes	However, targeted deletion of Mtf1 in the liver after birth is no longer lethal.
T319	426-433	RB	denotes	However
T321	433-435	,	denotes	,
T322	435-443	VBN	denotes	targeted
T323	444-452	NN	denotes	deletion
T324	453-455	IN	denotes	of
T325	456-460	NN	denotes	Mtf1
T326	461-463	IN	denotes	in
T327	464-467	DT	denotes	the
T328	468-473	NN	denotes	liver
T329	474-479	IN	denotes	after
T330	480-485	NN	denotes	birth
T320	486-488	VBZ	denotes	is
T331	489-491	RB	denotes	no
T332	492-498	RB	denotes	longer
T333	499-505	JJ	denotes	lethal
T334	505-506	.	denotes	.
T335	506-659	sentence	denotes	For this study, Mtf1 conditional knockout mice and control littermates were both mock- or cadmium-treated and liver-specific transcription was analyzed.
T336	507-510	IN	denotes	For
T338	511-515	DT	denotes	this
T339	516-521	NN	denotes	study
T340	521-523	,	denotes	,
T341	523-527	NN	denotes	Mtf1
T343	528-539	JJ	denotes	conditional
T344	540-548	NN	denotes	knockout
T342	549-553	NNS	denotes	mice
T345	554-557	CC	denotes	and
T346	558-565	NN	denotes	control
T347	566-577	NNS	denotes	littermates
T348	578-582	VBD	denotes	were
T349	583-587	CC	denotes	both
T350	588-592	JJ	denotes	mock
T351	592-593	HYPH	denotes	-
T352	594-596	CC	denotes	or
T353	597-604	NN	denotes	cadmium
T354	604-605	HYPH	denotes	-
T337	605-612	VBN	denotes	treated
T355	613-616	CC	denotes	and
T356	617-622	NN	denotes	liver
T358	622-623	HYPH	denotes	-
T357	623-631	JJ	denotes	specific
T359	632-645	NN	denotes	transcription
T361	646-649	VBD	denotes	was
T360	650-658	VBN	denotes	analyzed
T362	658-659	.	denotes	.
T363	659-792	sentence	denotes	Besides the well-characterized metallothionein genes, several new MTF-1 target genes with MRE motifs in the promoter region emerged.
T364	660-667	IN	denotes	Besides
T366	668-671	DT	denotes	the
T368	672-676	RB	denotes	well
T370	676-677	HYPH	denotes	-
T369	677-690	VBN	denotes	characterized
T371	691-706	NN	denotes	metallothionein
T367	707-712	NNS	denotes	genes
T372	712-714	,	denotes	,
T373	714-721	JJ	denotes	several
T375	722-725	JJ	denotes	new
T376	726-729	NN	denotes	MTF
T377	729-730	HYPH	denotes	-
T378	730-731	CD	denotes	1
T379	732-738	NN	denotes	target
T374	739-744	NNS	denotes	genes
T380	745-749	IN	denotes	with
T381	750-753	NN	denotes	MRE
T382	754-760	NNS	denotes	motifs
T383	761-763	IN	denotes	in
T384	764-767	DT	denotes	the
T386	768-776	NN	denotes	promoter
T385	777-783	NN	denotes	region
T365	784-791	VBD	denotes	emerged
T387	791-792	.	denotes	.
T388	792-1007	sentence	denotes	MTF-1 is required for the basal expression of selenoprotein W, muscle 1 gene (Sepw1) that encodes a glutathione-binding and putative antioxidant protein, supporting a role of MTF-1 in the oxidative stress response.
T389	793-796	NN	denotes	MTF
T391	796-797	HYPH	denotes	-
T392	797-798	CD	denotes	1
T393	799-801	VBZ	denotes	is
T390	802-810	VBN	denotes	required
T394	811-814	IN	denotes	for
T395	815-818	DT	denotes	the
T397	819-824	JJ	denotes	basal
T396	825-835	NN	denotes	expression
T398	836-838	IN	denotes	of
T399	839-852	NN	denotes	selenoprotein
T401	853-854	NN	denotes	W
T402	854-856	,	denotes	,
T403	856-862	NN	denotes	muscle
T404	863-864	CD	denotes	1
T400	865-869	NN	denotes	gene
T405	870-871	-LRB-	denotes	(
T406	871-876	NN	denotes	Sepw1
T407	876-877	-RRB-	denotes	)
T408	878-882	WDT	denotes	that
T409	883-890	VBZ	denotes	encodes
T410	891-892	DT	denotes	a
T412	893-904	NN	denotes	glutathione
T414	904-905	HYPH	denotes	-
T413	905-912	VBG	denotes	binding
T415	913-916	CC	denotes	and
T416	917-925	JJ	denotes	putative
T417	926-937	NN	denotes	antioxidant
T411	938-945	NN	denotes	protein
T418	945-947	,	denotes	,
T419	947-957	VBG	denotes	supporting
T420	958-959	DT	denotes	a
T421	960-964	NN	denotes	role
T422	965-967	IN	denotes	of
T423	968-971	NN	denotes	MTF
T424	971-972	HYPH	denotes	-
T425	972-973	CD	denotes	1
T426	974-976	IN	denotes	in
T427	977-980	DT	denotes	the
T429	981-990	JJ	denotes	oxidative
T430	991-997	NN	denotes	stress
T428	998-1006	NN	denotes	response
T431	1006-1007	.	denotes	.
T432	1007-1196	sentence	denotes	Furthermore, MTF-1 mediates the cadmium-induced expression of N-myc downstream regulated gene 1 (Ndrg1), which is induced by several stress conditions and is overexpressed in many cancers.
T433	1008-1019	RB	denotes	Furthermore
T435	1019-1021	,	denotes	,
T436	1021-1024	NN	denotes	MTF
T437	1024-1025	HYPH	denotes	-
T438	1025-1026	CD	denotes	1
T434	1027-1035	VBZ	denotes	mediates
T439	1036-1039	DT	denotes	the
T441	1040-1047	NN	denotes	cadmium
T443	1047-1048	HYPH	denotes	-
T442	1048-1055	VBN	denotes	induced
T440	1056-1066	NN	denotes	expression
T444	1067-1069	IN	denotes	of
T445	1070-1071	NN	denotes	N
T447	1071-1072	HYPH	denotes	-
T446	1072-1075	NN	denotes	myc
T449	1076-1086	RB	denotes	downstream
T450	1087-1096	VBN	denotes	regulated
T448	1097-1101	NN	denotes	gene
T451	1102-1103	CD	denotes	1
T452	1104-1105	-LRB-	denotes	(
T453	1105-1110	NN	denotes	Ndrg1
T454	1110-1111	-RRB-	denotes	)
T455	1111-1113	,	denotes	,
T456	1113-1118	WDT	denotes	which
T458	1119-1121	VBZ	denotes	is
T457	1122-1129	VBN	denotes	induced
T459	1130-1132	IN	denotes	by
T460	1133-1140	JJ	denotes	several
T462	1141-1147	NN	denotes	stress
T461	1148-1158	NNS	denotes	conditions
T463	1159-1162	CC	denotes	and
T464	1163-1165	VBZ	denotes	is
T465	1166-1179	VBN	denotes	overexpressed
T466	1180-1182	IN	denotes	in
T467	1183-1187	JJ	denotes	many
T468	1188-1195	NNS	denotes	cancers
T469	1195-1196	.	denotes	.
T470	1196-1347	sentence	denotes	MTF-1 is also involved in the cadmium response of cysteine- and glycine-rich protein 1 gene (Csrp1), which is implicated in cytoskeletal organization.
T471	1197-1200	NN	denotes	MTF
T473	1200-1201	HYPH	denotes	-
T474	1201-1202	CD	denotes	1
T475	1203-1205	VBZ	denotes	is
T476	1206-1210	RB	denotes	also
T472	1211-1219	VBN	denotes	involved
T477	1220-1222	IN	denotes	in
T478	1223-1226	DT	denotes	the
T480	1227-1234	NN	denotes	cadmium
T479	1235-1243	NN	denotes	response
T481	1244-1246	IN	denotes	of
T482	1247-1255	NN	denotes	cysteine
T484	1255-1256	HYPH	denotes	-
T485	1257-1260	CC	denotes	and
T486	1261-1268	NN	denotes	glycine
T487	1268-1269	HYPH	denotes	-
T483	1269-1273	JJ	denotes	rich
T489	1274-1281	NN	denotes	protein
T490	1282-1283	CD	denotes	1
T488	1284-1288	NN	denotes	gene
T491	1289-1290	-LRB-	denotes	(
T492	1290-1295	NN	denotes	Csrp1
T493	1295-1296	-RRB-	denotes	)
T494	1296-1298	,	denotes	,
T495	1298-1303	WDT	denotes	which
T497	1304-1306	VBZ	denotes	is
T496	1307-1317	VBN	denotes	implicated
T498	1318-1320	IN	denotes	in
T499	1321-1333	JJ	denotes	cytoskeletal
T500	1334-1346	NN	denotes	organization
T501	1346-1347	.	denotes	.
T502	1347-1439	sentence	denotes	In contrast, MTF-1 represses the basal expression of Slc39a10, a putative zinc transporter.
T503	1348-1350	IN	denotes	In
T505	1351-1359	NN	denotes	contrast
T506	1359-1361	,	denotes	,
T507	1361-1364	NN	denotes	MTF
T508	1364-1365	HYPH	denotes	-
T509	1365-1366	CD	denotes	1
T504	1367-1376	VBZ	denotes	represses
T510	1377-1380	DT	denotes	the
T512	1381-1386	JJ	denotes	basal
T511	1387-1397	NN	denotes	expression
T513	1398-1400	IN	denotes	of
T514	1401-1409	NN	denotes	Slc39a10
T515	1409-1411	,	denotes	,
T516	1411-1412	DT	denotes	a
T518	1413-1421	JJ	denotes	putative
T519	1422-1426	NN	denotes	zinc
T517	1427-1438	NN	denotes	transporter
T520	1438-1439	.	denotes	.
T521	1439-1612	sentence	denotes	In a pathway independent of MTF-1, cadmium also induced the transcription of genes involved in the synthesis and regeneration of glutathione, a cadmium-binding antioxidant.
T522	1440-1442	IN	denotes	In
T524	1443-1444	DT	denotes	a
T525	1445-1452	NN	denotes	pathway
T526	1453-1464	JJ	denotes	independent
T527	1465-1467	IN	denotes	of
T528	1468-1471	NN	denotes	MTF
T529	1471-1472	HYPH	denotes	-
T530	1472-1473	CD	denotes	1
T531	1473-1475	,	denotes	,
T532	1475-1482	NN	denotes	cadmium
T533	1483-1487	RB	denotes	also
T523	1488-1495	VBD	denotes	induced
T534	1496-1499	DT	denotes	the
T535	1500-1513	NN	denotes	transcription
T536	1514-1516	IN	denotes	of
T537	1517-1522	NNS	denotes	genes
T538	1523-1531	VBN	denotes	involved
T539	1532-1534	IN	denotes	in
T540	1535-1538	DT	denotes	the
T541	1539-1548	NN	denotes	synthesis
T542	1549-1552	CC	denotes	and
T543	1553-1565	NN	denotes	regeneration
T544	1566-1568	IN	denotes	of
T545	1569-1580	NN	denotes	glutathione
T546	1580-1582	,	denotes	,
T547	1582-1583	DT	denotes	a
T549	1584-1591	NN	denotes	cadmium
T551	1591-1592	HYPH	denotes	-
T550	1592-1599	VBG	denotes	binding
T548	1600-1611	NN	denotes	antioxidant
T552	1611-1612	.	denotes	.
T553	1612-1838	sentence	denotes	These data provide strong evidence for two major branches of cellular anti-cadmium defense, one via MTF-1 and its target genes, notably metallothioneins, the other via glutathione, with an apparent overlap in selenoprotein W.
T554	1613-1618	DT	denotes	These
T555	1619-1623	NNS	denotes	data
T556	1624-1631	VBP	denotes	provide
T557	1632-1638	JJ	denotes	strong
T558	1639-1647	NN	denotes	evidence
T559	1648-1651	IN	denotes	for
T560	1652-1655	CD	denotes	two
T562	1656-1661	JJ	denotes	major
T561	1662-1670	NNS	denotes	branches
T563	1671-1673	IN	denotes	of
T564	1674-1682	JJ	denotes	cellular
T566	1683-1695	JJ	denotes	anti-cadmium
T565	1696-1703	NN	denotes	defense
T567	1703-1705	,	denotes	,
T568	1705-1708	CD	denotes	one
T569	1709-1712	IN	denotes	via
T570	1713-1716	NN	denotes	MTF
T571	1716-1717	HYPH	denotes	-
T572	1717-1718	CD	denotes	1
T573	1719-1722	CC	denotes	and
T574	1723-1726	PRP$	denotes	its
T576	1727-1733	NN	denotes	target
T575	1734-1739	NNS	denotes	genes
T577	1739-1741	,	denotes	,
T578	1741-1748	RB	denotes	notably
T579	1749-1765	NNS	denotes	metallothioneins
T580	1765-1767	,	denotes	,
T581	1767-1770	DT	denotes	the
T582	1771-1776	JJ	denotes	other
T583	1777-1780	IN	denotes	via
T584	1781-1792	NN	denotes	glutathione
T585	1792-1794	,	denotes	,
T586	1794-1798	IN	denotes	with
T587	1799-1801	DT	denotes	an
T589	1802-1810	JJ	denotes	apparent
T588	1811-1818	NN	denotes	overlap
T590	1819-1821	IN	denotes	in
T591	1822-1835	NN	denotes	selenoprotein
T592	1836-1837	NN	denotes	W
T593	1837-1838	.	denotes	.
T980	1853-1856	DT	denotes	All
T981	1857-1866	NNS	denotes	organisms
T983	1867-1871	VBP	denotes	have
T982	1872-1879	VBN	denotes	evolved
T984	1880-1890	NNS	denotes	mechanisms
T985	1891-1893	TO	denotes	to
T986	1894-1898	VB	denotes	cope
T987	1899-1903	IN	denotes	with
T988	1904-1905	DT	denotes	a
T989	1906-1913	NN	denotes	variety
T990	1914-1916	IN	denotes	of
T991	1917-1923	NN	denotes	stress
T992	1924-1934	NNS	denotes	situations
T993	1934-1935	.	denotes	.
T994	1935-2144	sentence	denotes	One type of stress response is triggered by heavy metals, such as zinc, copper and cadmium (for convenience, the terms zinc, copper and cadmium are also used here to denote Zn2+, Cu2+ and Cd2+, respectively).
T995	1936-1939	CD	denotes	One
T996	1940-1944	NN	denotes	type
T998	1945-1947	IN	denotes	of
T999	1948-1954	NN	denotes	stress
T1000	1955-1963	NN	denotes	response
T1001	1964-1966	VBZ	denotes	is
T997	1967-1976	VBN	denotes	triggered
T1002	1977-1979	IN	denotes	by
T1003	1980-1985	JJ	denotes	heavy
T1004	1986-1992	NNS	denotes	metals
T1005	1992-1994	,	denotes	,
T1006	1994-1998	JJ	denotes	such
T1007	1999-2001	IN	denotes	as
T1008	2002-2006	NN	denotes	zinc
T1009	2006-2008	,	denotes	,
T1010	2008-2014	NN	denotes	copper
T1011	2015-2018	CC	denotes	and
T1012	2019-2026	NN	denotes	cadmium
T1013	2027-2028	-LRB-	denotes	(
T1015	2028-2031	IN	denotes	for
T1016	2032-2043	NN	denotes	convenience
T1017	2043-2045	,	denotes	,
T1018	2045-2048	DT	denotes	the
T1020	2049-2054	NNS	denotes	terms
T1019	2055-2059	NN	denotes	zinc
T1021	2059-2061	,	denotes	,
T1022	2061-2067	NN	denotes	copper
T1023	2068-2071	CC	denotes	and
T1024	2072-2079	NN	denotes	cadmium
T1025	2080-2083	VBP	denotes	are
T1026	2084-2088	RB	denotes	also
T1014	2089-2093	VBN	denotes	used
T1027	2094-2098	RB	denotes	here
T1028	2099-2101	TO	denotes	to
T1029	2102-2108	VB	denotes	denote
T1030	2109-2112	NN	denotes	Zn2
T1031	2112-2113	SYM	denotes	+
T1032	2113-2115	,	denotes	,
T1033	2115-2118	NN	denotes	Cu2
T1034	2118-2119	SYM	denotes	+
T1035	2120-2123	CC	denotes	and
T1036	2124-2127	NN	denotes	Cd2
T1037	2127-2128	SYM	denotes	+
T1038	2128-2130	,	denotes	,
T1039	2130-2142	RB	denotes	respectively
T1040	2142-2143	-RRB-	denotes	)
T1041	2143-2144	.	denotes	.
T1042	2144-2325	sentence	denotes	Metallothioneins (MTs), small, cysteine-rich proteins, play an important role in metal homeostasis and detoxification due to their ability to bind different heavy metal ions (1–3).
T1043	2145-2161	NNS	denotes	Metallothioneins
T1045	2162-2163	-LRB-	denotes	(
T1046	2163-2166	NNS	denotes	MTs
T1047	2166-2167	-RRB-	denotes	)
T1048	2167-2169	,	denotes	,
T1049	2169-2174	JJ	denotes	small
T1051	2174-2176	,	denotes	,
T1052	2176-2184	NN	denotes	cysteine
T1054	2184-2185	HYPH	denotes	-
T1053	2185-2189	JJ	denotes	rich
T1050	2190-2198	NN	denotes	proteins
T1055	2198-2200	,	denotes	,
T1044	2200-2204	VBP	denotes	play
T1056	2205-2207	DT	denotes	an
T1058	2208-2217	JJ	denotes	important
T1057	2218-2222	NN	denotes	role
T1059	2223-2225	IN	denotes	in
T1060	2226-2231	NN	denotes	metal
T1061	2232-2243	NN	denotes	homeostasis
T1062	2244-2247	CC	denotes	and
T1063	2248-2262	NN	denotes	detoxification
T1064	2263-2266	IN	denotes	due
T1065	2267-2269	IN	denotes	to
T1066	2270-2275	PRP$	denotes	their
T1067	2276-2283	NN	denotes	ability
T1068	2284-2286	TO	denotes	to
T1069	2287-2291	VB	denotes	bind
T1070	2292-2301	JJ	denotes	different
T1072	2302-2307	JJ	denotes	heavy
T1073	2308-2313	NN	denotes	metal
T1071	2314-2318	NNS	denotes	ions
T1074	2319-2320	-LRB-	denotes	(
T1075	2320-2321	CD	denotes	1
T1076	2321-2322	SYM	denotes	–
T1077	2322-2323	CD	denotes	3
T1078	2323-2324	-RRB-	denotes	)
T1079	2324-2325	.	denotes	.
T1080	2325-2403	sentence	denotes	In the mouse, there are four metallothionein genes, designated as Mt1 to Mt4.
T1081	2326-2328	IN	denotes	In
T1083	2329-2332	DT	denotes	the
T1084	2333-2338	NN	denotes	mouse
T1085	2338-2340	,	denotes	,
T1086	2340-2345	EX	denotes	there
T1082	2346-2349	VBP	denotes	are
T1087	2350-2354	CD	denotes	four
T1089	2355-2370	NN	denotes	metallothionein
T1088	2371-2376	NNS	denotes	genes
T1090	2376-2378	,	denotes	,
T1091	2378-2388	VBN	denotes	designated
T1092	2389-2391	IN	denotes	as
T1093	2392-2395	NN	denotes	Mt1
T1094	2396-2398	IN	denotes	to
T1095	2399-2402	NN	denotes	Mt4
T1096	2402-2403	.	denotes	.
T1097	2403-2538	sentence	denotes	Basal, as well as heavy metal-induced, expression of Mt1 and Mt2 is mediated by metal-responsive transcription factor 1 (MTF-1) (4–7).
T1098	2404-2409	JJ	denotes	Basal
T1100	2409-2411	,	denotes	,
T1101	2411-2413	RB	denotes	as
T1103	2414-2418	RB	denotes	well
T1102	2419-2421	IN	denotes	as
T1104	2422-2427	JJ	denotes	heavy
T1105	2428-2433	NN	denotes	metal
T1107	2433-2434	HYPH	denotes	-
T1106	2434-2441	VBN	denotes	induced
T1108	2441-2443	,	denotes	,
T1099	2443-2453	NN	denotes	expression
T1110	2454-2456	IN	denotes	of
T1111	2457-2460	NN	denotes	Mt1
T1112	2461-2464	CC	denotes	and
T1113	2465-2468	NN	denotes	Mt2
T1114	2469-2471	VBZ	denotes	is
T1109	2472-2480	VBN	denotes	mediated
T1115	2481-2483	IN	denotes	by
T1116	2484-2489	NN	denotes	metal
T1118	2489-2490	HYPH	denotes	-
T1117	2490-2500	JJ	denotes	responsive
T1120	2501-2514	NN	denotes	transcription
T1119	2515-2521	NN	denotes	factor
T1121	2522-2523	CD	denotes	1
T1122	2524-2525	-LRB-	denotes	(
T1123	2525-2528	NN	denotes	MTF
T1124	2528-2529	HYPH	denotes	-
T1125	2529-2530	CD	denotes	1
T1126	2530-2531	-RRB-	denotes	)
T1127	2532-2533	-LRB-	denotes	(
T1128	2533-2534	CD	denotes	4
T1129	2534-2535	SYM	denotes	–
T1130	2535-2536	CD	denotes	7
T1131	2536-2537	-RRB-	denotes	)
T1132	2537-2538	.	denotes	.
T1133	2538-2736	sentence	denotes	This zinc finger protein recognizes short cis-acting DNA sequences, termed metal response elements (MREs; core consensus sequence TGCRCNC), which are present in the promoters of target genes (8,9).
T1134	2539-2543	DT	denotes	This
T1136	2544-2548	NN	denotes	zinc
T1137	2549-2555	NN	denotes	finger
T1135	2556-2563	NN	denotes	protein
T1138	2564-2574	VBZ	denotes	recognizes
T1139	2575-2580	JJ	denotes	short
T1141	2581-2584	NN	denotes	cis
T1143	2584-2585	HYPH	denotes	-
T1142	2585-2591	VBG	denotes	acting
T1144	2592-2595	NN	denotes	DNA
T1140	2596-2605	NNS	denotes	sequences
T1145	2605-2607	,	denotes	,
T1146	2607-2613	VBN	denotes	termed
T1147	2614-2619	NN	denotes	metal
T1149	2620-2628	NN	denotes	response
T1148	2629-2637	NNS	denotes	elements
T1150	2638-2639	-LRB-	denotes	(
T1151	2639-2643	NNS	denotes	MREs
T1152	2643-2644	:	denotes	;
T1154	2645-2649	NN	denotes	core
T1155	2650-2659	NN	denotes	consensus
T1156	2660-2668	NN	denotes	sequence
T1153	2669-2676	NN	denotes	TGCRCNC
T1157	2676-2677	-RRB-	denotes	)
T1158	2677-2679	,	denotes	,
T1159	2679-2684	WDT	denotes	which
T1160	2685-2688	VBP	denotes	are
T1161	2689-2696	JJ	denotes	present
T1162	2697-2699	IN	denotes	in
T1163	2700-2703	DT	denotes	the
T1164	2704-2713	NNS	denotes	promoters
T1165	2714-2716	IN	denotes	of
T1166	2717-2723	NN	denotes	target
T1167	2724-2729	NNS	denotes	genes
T1168	2730-2731	-LRB-	denotes	(
T1170	2731-2732	CD	denotes	8
T1171	2732-2733	,	denotes	,
T1169	2733-2734	CD	denotes	9
T1172	2734-2735	-RRB-	denotes	)
T1173	2735-2736	.	denotes	.
T1174	2736-2875	sentence	denotes	MTF-1 is conserved in evolution, and homologs have been characterized in the mouse (10), humans (11), Drosophila (12–14) and fish (15,16).
T1175	2737-2740	NN	denotes	MTF
T1177	2740-2741	HYPH	denotes	-
T1178	2741-2742	CD	denotes	1
T1179	2743-2745	VBZ	denotes	is
T1176	2746-2755	VBN	denotes	conserved
T1180	2756-2758	IN	denotes	in
T1181	2759-2768	NN	denotes	evolution
T1182	2768-2770	,	denotes	,
T1183	2770-2773	CC	denotes	and
T1184	2774-2782	NNS	denotes	homologs
T1186	2783-2787	VBP	denotes	have
T1187	2788-2792	VBN	denotes	been
T1185	2793-2806	VBN	denotes	characterized
T1188	2807-2809	IN	denotes	in
T1189	2810-2813	DT	denotes	the
T1190	2814-2819	NN	denotes	mouse
T1191	2820-2821	-LRB-	denotes	(
T1192	2821-2823	CD	denotes	10
T1193	2823-2824	-RRB-	denotes	)
T1194	2824-2826	,	denotes	,
T1195	2826-2832	NNS	denotes	humans
T1196	2833-2834	-LRB-	denotes	(
T1197	2834-2836	CD	denotes	11
T1198	2836-2837	-RRB-	denotes	)
T1199	2837-2839	,	denotes	,
T1200	2839-2849	NNP	denotes	Drosophila
T1201	2850-2851	-LRB-	denotes	(
T1202	2851-2853	CD	denotes	12
T1203	2853-2854	SYM	denotes	–
T1204	2854-2856	CD	denotes	14
T1205	2856-2857	-RRB-	denotes	)
T1206	2858-2861	CC	denotes	and
T1207	2862-2866	NN	denotes	fish
T1208	2867-2868	-LRB-	denotes	(
T1210	2868-2870	CD	denotes	15
T1211	2870-2871	,	denotes	,
T1209	2871-2873	CD	denotes	16
T1212	2873-2874	-RRB-	denotes	)
T1213	2874-2875	.	denotes	.
T1214	2875-2941	sentence	denotes	The role of MTF-1 has been studied most extensively in the mouse.
T1215	2876-2879	DT	denotes	The
T1216	2880-2884	NN	denotes	role
T1218	2885-2887	IN	denotes	of
T1219	2888-2891	NN	denotes	MTF
T1220	2891-2892	HYPH	denotes	-
T1221	2892-2893	CD	denotes	1
T1222	2894-2897	VBZ	denotes	has
T1223	2898-2902	VBN	denotes	been
T1217	2903-2910	VBN	denotes	studied
T1224	2911-2915	RBS	denotes	most
T1225	2916-2927	RB	denotes	extensively
T1226	2928-2930	IN	denotes	in
T1227	2931-2934	DT	denotes	the
T1228	2935-2940	NN	denotes	mouse
T1229	2940-2941	.	denotes	.
T1230	2941-3118	sentence	denotes	Besides coping with heavy metal load, MTF-1 can also mediate the induction of Mt genes in response to other stress situations, such as oxidative stress (5,17) and hypoxia (18).
T1231	2942-2949	IN	denotes	Besides
T1233	2950-2956	VBG	denotes	coping
T1234	2957-2961	IN	denotes	with
T1235	2962-2967	JJ	denotes	heavy
T1236	2968-2973	NN	denotes	metal
T1237	2974-2978	NN	denotes	load
T1238	2978-2980	,	denotes	,
T1239	2980-2983	NN	denotes	MTF
T1240	2983-2984	HYPH	denotes	-
T1241	2984-2985	CD	denotes	1
T1242	2986-2989	MD	denotes	can
T1243	2990-2994	RB	denotes	also
T1232	2995-3002	VB	denotes	mediate
T1244	3003-3006	DT	denotes	the
T1245	3007-3016	NN	denotes	induction
T1246	3017-3019	IN	denotes	of
T1247	3020-3022	NN	denotes	Mt
T1248	3023-3028	NNS	denotes	genes
T1249	3029-3031	IN	denotes	in
T1250	3032-3040	NN	denotes	response
T1251	3041-3043	IN	denotes	to
T1252	3044-3049	JJ	denotes	other
T1254	3050-3056	NN	denotes	stress
T1253	3057-3067	NNS	denotes	situations
T1255	3067-3069	,	denotes	,
T1256	3069-3073	JJ	denotes	such
T1257	3074-3076	IN	denotes	as
T1258	3077-3086	JJ	denotes	oxidative
T1259	3087-3093	NN	denotes	stress
T1260	3094-3095	-LRB-	denotes	(
T1262	3095-3096	CD	denotes	5
T1263	3096-3097	,	denotes	,
T1261	3097-3099	CD	denotes	17
T1264	3099-3100	-RRB-	denotes	)
T1265	3101-3104	CC	denotes	and
T1266	3105-3112	NN	denotes	hypoxia
T1267	3113-3114	-LRB-	denotes	(
T1268	3114-3116	CD	denotes	18
T1269	3116-3117	-RRB-	denotes	)
T1270	3117-3118	.	denotes	.
T1271	3118-3477	sentence	denotes	In addition, it is required for the metalloregulation of Znt1, encoding the major plasma membrane-localized zinc efflux transporter (19), the hypoxic/anoxic induction of the gene for placental growth factor (Plgf), an angiogenic protein of the vascular endothelial growth factor (VEGF) family (20), and has recently been invoked in tumor development (21,22).
T1272	3119-3121	IN	denotes	In
T1274	3122-3130	NN	denotes	addition
T1275	3130-3132	,	denotes	,
T1276	3132-3134	PRP	denotes	it
T1277	3135-3137	VBZ	denotes	is
T1273	3138-3146	VBN	denotes	required
T1278	3147-3150	IN	denotes	for
T1279	3151-3154	DT	denotes	the
T1280	3155-3172	NN	denotes	metalloregulation
T1281	3173-3175	IN	denotes	of
T1282	3176-3180	NN	denotes	Znt1
T1283	3180-3182	,	denotes	,
T1284	3182-3190	VBG	denotes	encoding
T1285	3191-3194	DT	denotes	the
T1287	3195-3200	JJ	denotes	major
T1288	3201-3207	NN	denotes	plasma
T1289	3208-3216	NN	denotes	membrane
T1291	3216-3217	HYPH	denotes	-
T1290	3217-3226	VBN	denotes	localized
T1292	3227-3231	NN	denotes	zinc
T1293	3232-3238	NN	denotes	efflux
T1286	3239-3250	NN	denotes	transporter
T1294	3251-3252	-LRB-	denotes	(
T1295	3252-3254	CD	denotes	19
T1296	3254-3255	-RRB-	denotes	)
T1297	3255-3257	,	denotes	,
T1298	3257-3260	DT	denotes	the
T1300	3261-3268	JJ	denotes	hypoxic
T1302	3268-3269	HYPH	denotes	/
T1301	3269-3275	JJ	denotes	anoxic
T1299	3276-3285	NN	denotes	induction
T1303	3286-3288	IN	denotes	of
T1304	3289-3292	DT	denotes	the
T1305	3293-3297	NN	denotes	gene
T1306	3298-3301	IN	denotes	for
T1307	3302-3311	JJ	denotes	placental
T1309	3312-3318	NN	denotes	growth
T1308	3319-3325	NN	denotes	factor
T1310	3326-3327	-LRB-	denotes	(
T1311	3327-3331	NN	denotes	Plgf
T1312	3331-3332	-RRB-	denotes	)
T1313	3332-3334	,	denotes	,
T1314	3334-3336	DT	denotes	an
T1316	3337-3347	JJ	denotes	angiogenic
T1315	3348-3355	NN	denotes	protein
T1317	3356-3358	IN	denotes	of
T1318	3359-3362	DT	denotes	the
T1320	3363-3371	JJ	denotes	vascular
T1322	3372-3383	JJ	denotes	endothelial
T1323	3384-3390	NN	denotes	growth
T1321	3391-3397	NN	denotes	factor
T1324	3398-3399	-LRB-	denotes	(
T1325	3399-3403	NN	denotes	VEGF
T1326	3403-3404	-RRB-	denotes	)
T1319	3405-3411	NN	denotes	family
T1327	3412-3413	-LRB-	denotes	(
T1328	3413-3415	CD	denotes	20
T1329	3415-3416	-RRB-	denotes	)
T1330	3416-3418	,	denotes	,
T1331	3418-3421	CC	denotes	and
T1332	3422-3425	VBZ	denotes	has
T1334	3426-3434	RB	denotes	recently
T1335	3435-3439	VBN	denotes	been
T1333	3440-3447	VBN	denotes	invoked
T1336	3448-3450	IN	denotes	in
T1337	3451-3456	NN	denotes	tumor
T1338	3457-3468	NN	denotes	development
T1339	3469-3470	-LRB-	denotes	(
T1341	3470-3472	CD	denotes	21
T1342	3472-3473	,	denotes	,
T1340	3473-3475	CD	denotes	22
T1343	3475-3476	-RRB-	denotes	)
T1344	3476-3477	.	denotes	.
T1345	3477-3663	sentence	denotes	Furthermore, MTF-1 has an essential function during embryogenesis: targeted disruption of Mtf1 results in embryonic lethality around 14 days post coitum due to hepatocyte necrosis (23).
T1346	3478-3489	RB	denotes	Furthermore
T1348	3489-3491	,	denotes	,
T1349	3491-3494	NN	denotes	MTF
T1350	3494-3495	HYPH	denotes	-
T1351	3495-3496	CD	denotes	1
T1347	3497-3500	VBZ	denotes	has
T1353	3501-3503	DT	denotes	an
T1355	3504-3513	JJ	denotes	essential
T1354	3514-3522	NN	denotes	function
T1356	3523-3529	IN	denotes	during
T1357	3530-3543	NN	denotes	embryogenesis
T1358	3543-3545	:	denotes	:
T1359	3545-3553	VBN	denotes	targeted
T1360	3554-3564	NN	denotes	disruption
T1361	3565-3567	IN	denotes	of
T1362	3568-3572	NN	denotes	Mtf1
T1352	3573-3580	VBZ	denotes	results
T1363	3581-3583	IN	denotes	in
T1364	3584-3593	JJ	denotes	embryonic
T1365	3594-3603	NN	denotes	lethality
T1366	3604-3610	IN	denotes	around
T1367	3611-3613	CD	denotes	14
T1368	3614-3618	NNS	denotes	days
T1369	3619-3623	FW	denotes	post
T1370	3624-3630	FW	denotes	coitum
T1371	3631-3634	IN	denotes	due
T1372	3635-3637	IN	denotes	to
T1373	3638-3648	NN	denotes	hepatocyte
T1374	3649-3657	NN	denotes	necrosis
T1375	3658-3659	-LRB-	denotes	(
T1376	3659-3661	CD	denotes	23
T1377	3661-3662	-RRB-	denotes	)
T1378	3662-3663	.	denotes	.
T1379	3663-3905	sentence	denotes	In contrast, mice with null mutations for the stress-inducible metallothionein genes (Mt1 and Mt2) are viable, though sensitive to cadmium (24,25), indicating that additional important MTF-1 target genes are involved in the lethal phenotype.
T1380	3664-3666	IN	denotes	In
T1382	3667-3675	NN	denotes	contrast
T1383	3675-3677	,	denotes	,
T1384	3677-3681	NNS	denotes	mice
T1385	3682-3686	IN	denotes	with
T1386	3687-3691	JJ	denotes	null
T1387	3692-3701	NNS	denotes	mutations
T1388	3702-3705	IN	denotes	for
T1389	3706-3709	DT	denotes	the
T1391	3710-3716	NN	denotes	stress
T1393	3716-3717	HYPH	denotes	-
T1392	3717-3726	JJ	denotes	inducible
T1394	3727-3742	NN	denotes	metallothionein
T1390	3743-3748	NNS	denotes	genes
T1395	3749-3750	-LRB-	denotes	(
T1396	3750-3753	NN	denotes	Mt1
T1397	3754-3757	CC	denotes	and
T1398	3758-3761	NN	denotes	Mt2
T1399	3761-3762	-RRB-	denotes	)
T1381	3763-3766	VBP	denotes	are
T1400	3767-3773	JJ	denotes	viable
T1401	3773-3775	,	denotes	,
T1402	3775-3781	IN	denotes	though
T1403	3782-3791	JJ	denotes	sensitive
T1404	3792-3794	IN	denotes	to
T1405	3795-3802	NN	denotes	cadmium
T1406	3803-3804	-LRB-	denotes	(
T1408	3804-3806	CD	denotes	24
T1409	3806-3807	,	denotes	,
T1407	3807-3809	CD	denotes	25
T1410	3809-3810	-RRB-	denotes	)
T1411	3810-3812	,	denotes	,
T1412	3812-3822	VBG	denotes	indicating
T1413	3823-3827	IN	denotes	that
T1415	3828-3838	JJ	denotes	additional
T1417	3839-3848	JJ	denotes	important
T1418	3849-3852	NN	denotes	MTF
T1419	3852-3853	HYPH	denotes	-
T1420	3853-3854	CD	denotes	1
T1421	3855-3861	NN	denotes	target
T1416	3862-3867	NNS	denotes	genes
T1422	3868-3871	VBP	denotes	are
T1414	3872-3880	VBN	denotes	involved
T1423	3881-3883	IN	denotes	in
T1424	3884-3887	DT	denotes	the
T1426	3888-3894	JJ	denotes	lethal
T1425	3895-3904	NN	denotes	phenotype
T1427	3904-3905	.	denotes	.
T1428	3905-4051	sentence	denotes	With the Cre-loxP conditional knockout technique, it is possible to circumvent the embryonic lethal phenotype of conventional Mtf1 knockout mice.
T1429	3906-3910	IN	denotes	With
T1431	3911-3914	DT	denotes	the
T1433	3915-3918	NN	denotes	Cre
T1435	3918-3919	HYPH	denotes	-
T1434	3919-3923	NN	denotes	loxP
T1436	3924-3935	JJ	denotes	conditional
T1437	3936-3944	NN	denotes	knockout
T1432	3945-3954	NN	denotes	technique
T1438	3954-3956	,	denotes	,
T1439	3956-3958	PRP	denotes	it
T1430	3959-3961	VBZ	denotes	is
T1440	3962-3970	JJ	denotes	possible
T1441	3971-3973	TO	denotes	to
T1442	3974-3984	VB	denotes	circumvent
T1443	3985-3988	DT	denotes	the
T1445	3989-3998	JJ	denotes	embryonic
T1446	3999-4005	JJ	denotes	lethal
T1444	4006-4015	NN	denotes	phenotype
T1447	4016-4018	IN	denotes	of
T1448	4019-4031	JJ	denotes	conventional
T1450	4032-4036	NN	denotes	Mtf1
T1451	4037-4045	NN	denotes	knockout
T1449	4046-4050	NNS	denotes	mice
T1452	4050-4051	.	denotes	.
T1453	4051-4204	sentence	denotes	Previous experiments with this technique revealed that deletion of Mtf1 from the liver after birth is no longer lethal under non-stress conditions (26).
T1454	4052-4060	JJ	denotes	Previous
T1455	4061-4072	NNS	denotes	experiments
T1457	4073-4077	IN	denotes	with
T1458	4078-4082	DT	denotes	this
T1459	4083-4092	NN	denotes	technique
T1456	4093-4101	VBD	denotes	revealed
T1460	4102-4106	IN	denotes	that
T1462	4107-4115	NN	denotes	deletion
T1463	4116-4118	IN	denotes	of
T1464	4119-4123	NN	denotes	Mtf1
T1465	4124-4128	IN	denotes	from
T1466	4129-4132	DT	denotes	the
T1467	4133-4138	NN	denotes	liver
T1468	4139-4144	IN	denotes	after
T1469	4145-4150	NN	denotes	birth
T1461	4151-4153	VBZ	denotes	is
T1470	4154-4156	RB	denotes	no
T1471	4157-4163	RB	denotes	longer
T1472	4164-4170	JJ	denotes	lethal
T1473	4171-4176	IN	denotes	under
T1474	4177-4187	JJ	denotes	non-stress
T1475	4188-4198	NNS	denotes	conditions
T1476	4199-4200	-LRB-	denotes	(
T1477	4200-4202	CD	denotes	26
T1478	4202-4203	-RRB-	denotes	)
T1479	4203-4204	.	denotes	.
T1480	4204-4379	sentence	denotes	For this study, an inducible, liver-specific Mtf1 knockout mouse line was generated to perform a search for MTF-1 target genes and cadmium-inducible genes in the adult liver.
T1481	4205-4208	IN	denotes	For
T1483	4209-4213	DT	denotes	this
T1484	4214-4219	NN	denotes	study
T1485	4219-4221	,	denotes	,
T1486	4221-4223	DT	denotes	an
T1488	4224-4233	JJ	denotes	inducible
T1489	4233-4235	,	denotes	,
T1490	4235-4240	NN	denotes	liver
T1492	4240-4241	HYPH	denotes	-
T1491	4241-4249	JJ	denotes	specific
T1493	4250-4254	NN	denotes	Mtf1
T1494	4255-4263	NN	denotes	knockout
T1495	4264-4269	NN	denotes	mouse
T1487	4270-4274	NN	denotes	line
T1496	4275-4278	VBD	denotes	was
T1482	4279-4288	VBN	denotes	generated
T1497	4289-4291	TO	denotes	to
T1498	4292-4299	VB	denotes	perform
T1499	4300-4301	DT	denotes	a
T1500	4302-4308	NN	denotes	search
T1501	4309-4312	IN	denotes	for
T1502	4313-4316	NN	denotes	MTF
T1504	4316-4317	HYPH	denotes	-
T1505	4317-4318	CD	denotes	1
T1506	4319-4325	NN	denotes	target
T1503	4326-4331	NNS	denotes	genes
T1507	4332-4335	CC	denotes	and
T1508	4336-4343	NN	denotes	cadmium
T1510	4343-4344	HYPH	denotes	-
T1509	4344-4353	JJ	denotes	inducible
T1511	4354-4359	NNS	denotes	genes
T1512	4360-4362	IN	denotes	in
T1513	4363-4366	DT	denotes	the
T1515	4367-4372	JJ	denotes	adult
T1514	4373-4378	NN	denotes	liver
T1516	4378-4379	.	denotes	.
T1517	4379-4600	sentence	denotes	A number of target gene candidates emerged upon a transcriptome analysis of mock- and cadmium-treated Mtf1 conditional knockout mice and control littermates and several of these were confirmed by semiquantitative RT–PCR.
T1518	4380-4381	DT	denotes	A
T1519	4382-4388	NN	denotes	number
T1521	4389-4391	IN	denotes	of
T1522	4392-4398	NN	denotes	target
T1523	4399-4403	NN	denotes	gene
T1524	4404-4414	NNS	denotes	candidates
T1520	4415-4422	VBD	denotes	emerged
T1525	4423-4427	IN	denotes	upon
T1526	4428-4429	DT	denotes	a
T1528	4430-4443	NN	denotes	transcriptome
T1527	4444-4452	NN	denotes	analysis
T1529	4453-4455	IN	denotes	of
T1530	4456-4460	JJ	denotes	mock
T1532	4460-4461	HYPH	denotes	-
T1533	4462-4465	CC	denotes	and
T1534	4466-4473	NN	denotes	cadmium
T1535	4473-4474	HYPH	denotes	-
T1531	4474-4481	VBN	denotes	treated
T1537	4482-4486	NN	denotes	Mtf1
T1538	4487-4498	JJ	denotes	conditional
T1539	4499-4507	NN	denotes	knockout
T1536	4508-4512	NNS	denotes	mice
T1540	4513-4516	CC	denotes	and
T1541	4517-4524	NN	denotes	control
T1542	4525-4536	NNS	denotes	littermates
T1543	4537-4540	CC	denotes	and
T1544	4541-4548	JJ	denotes	several
T1546	4549-4551	IN	denotes	of
T1547	4552-4557	DT	denotes	these
T1548	4558-4562	VBD	denotes	were
T1545	4563-4572	VBN	denotes	confirmed
T1549	4573-4575	IN	denotes	by
T1550	4576-4592	JJ	denotes	semiquantitative
T1552	4593-4595	NN	denotes	RT
T1553	4595-4596	HYPH	denotes	–
T1551	4596-4599	NN	denotes	PCR
T1554	4599-4600	.	denotes	.
T1555	4600-4965	sentence	denotes	Besides the stress-inducible metallothionein genes that were already known as target genes of MTF-1, we find that MTF-1 is important for basal liver expression of the gene for selenoprotein W, muscle 1 (Sepw1) as well as for cadmium-induced expression of N-myc downstream regulated gene 1 (Ndrg1) and the gene encoding cysteine- and glycine-rich protein 1 (Csrp1).
T1556	4601-4608	IN	denotes	Besides
T1558	4609-4612	DT	denotes	the
T1560	4613-4619	NN	denotes	stress
T1562	4619-4620	HYPH	denotes	-
T1561	4620-4629	JJ	denotes	inducible
T1563	4630-4645	NN	denotes	metallothionein
T1559	4646-4651	NNS	denotes	genes
T1564	4652-4656	WDT	denotes	that
T1566	4657-4661	VBD	denotes	were
T1567	4662-4669	RB	denotes	already
T1565	4670-4675	VBN	denotes	known
T1568	4676-4678	IN	denotes	as
T1569	4679-4685	NN	denotes	target
T1570	4686-4691	NNS	denotes	genes
T1571	4692-4694	IN	denotes	of
T1572	4695-4698	NN	denotes	MTF
T1573	4698-4699	HYPH	denotes	-
T1574	4699-4700	CD	denotes	1
T1575	4700-4702	,	denotes	,
T1576	4702-4704	PRP	denotes	we
T1557	4705-4709	VBP	denotes	find
T1577	4710-4714	IN	denotes	that
T1579	4715-4718	NN	denotes	MTF
T1580	4718-4719	HYPH	denotes	-
T1581	4719-4720	CD	denotes	1
T1578	4721-4723	VBZ	denotes	is
T1582	4724-4733	JJ	denotes	important
T1583	4734-4737	IN	denotes	for
T1584	4738-4743	JJ	denotes	basal
T1586	4744-4749	NN	denotes	liver
T1585	4750-4760	NN	denotes	expression
T1587	4761-4763	IN	denotes	of
T1588	4764-4767	DT	denotes	the
T1589	4768-4772	NN	denotes	gene
T1590	4773-4776	IN	denotes	for
T1591	4777-4790	NN	denotes	selenoprotein
T1593	4791-4792	NN	denotes	W
T1594	4792-4794	,	denotes	,
T1592	4794-4800	NN	denotes	muscle
T1595	4801-4802	CD	denotes	1
T1596	4803-4804	-LRB-	denotes	(
T1597	4804-4809	NN	denotes	Sepw1
T1598	4809-4810	-RRB-	denotes	)
T1599	4811-4813	RB	denotes	as
T1601	4814-4818	RB	denotes	well
T1600	4819-4821	IN	denotes	as
T1602	4822-4825	IN	denotes	for
T1603	4826-4833	NN	denotes	cadmium
T1605	4833-4834	HYPH	denotes	-
T1604	4834-4841	VBN	denotes	induced
T1606	4842-4852	NN	denotes	expression
T1607	4853-4855	IN	denotes	of
T1608	4856-4857	NN	denotes	N
T1610	4857-4858	HYPH	denotes	-
T1609	4858-4861	NN	denotes	myc
T1612	4862-4872	RB	denotes	downstream
T1613	4873-4882	VBN	denotes	regulated
T1611	4883-4887	NN	denotes	gene
T1614	4888-4889	CD	denotes	1
T1615	4890-4891	-LRB-	denotes	(
T1616	4891-4896	NN	denotes	Ndrg1
T1617	4896-4897	-RRB-	denotes	)
T1618	4898-4901	CC	denotes	and
T1619	4902-4905	DT	denotes	the
T1620	4906-4910	NN	denotes	gene
T1621	4911-4919	VBG	denotes	encoding
T1622	4920-4928	NN	denotes	cysteine
T1624	4928-4929	HYPH	denotes	-
T1625	4930-4933	CC	denotes	and
T1626	4934-4941	NN	denotes	glycine
T1627	4941-4942	HYPH	denotes	-
T1623	4942-4946	JJ	denotes	rich
T1628	4947-4954	NN	denotes	protein
T1629	4955-4956	CD	denotes	1
T1630	4957-4958	-LRB-	denotes	(
T1631	4958-4963	NN	denotes	Csrp1
T1632	4963-4964	-RRB-	denotes	)
T1633	4964-4965	.	denotes	.
T1634	4965-5122	sentence	denotes	In addition, MTF-1 appears to repress the expression of solute carrier family 39, member 10 gene (Slc39a10), which encodes a putative metal ion transporter.
T1635	4966-4968	IN	denotes	In
T1637	4969-4977	NN	denotes	addition
T1638	4977-4979	,	denotes	,
T1639	4979-4982	NN	denotes	MTF
T1640	4982-4983	HYPH	denotes	-
T1641	4983-4984	CD	denotes	1
T1636	4985-4992	VBZ	denotes	appears
T1642	4993-4995	TO	denotes	to
T1643	4996-5003	VB	denotes	repress
T1644	5004-5007	DT	denotes	the
T1645	5008-5018	NN	denotes	expression
T1646	5019-5021	IN	denotes	of
T1647	5022-5028	NN	denotes	solute
T1649	5029-5036	NN	denotes	carrier
T1650	5037-5043	NN	denotes	family
T1651	5044-5046	CD	denotes	39
T1652	5046-5048	,	denotes	,
T1648	5048-5054	NN	denotes	member
T1654	5055-5057	CD	denotes	10
T1653	5058-5062	NN	denotes	gene
T1655	5063-5064	-LRB-	denotes	(
T1656	5064-5072	NN	denotes	Slc39a10
T1657	5072-5073	-RRB-	denotes	)
T1658	5073-5075	,	denotes	,
T1659	5075-5080	WDT	denotes	which
T1660	5081-5088	VBZ	denotes	encodes
T1661	5089-5090	DT	denotes	a
T1663	5091-5099	JJ	denotes	putative
T1664	5100-5105	NN	denotes	metal
T1665	5106-5109	NN	denotes	ion
T1662	5110-5121	NN	denotes	transporter
T1666	5121-5122	.	denotes	.
T1667	5122-5232	sentence	denotes	In an MTF-1-independent transcriptome response, several genes involved in glutathione metabolism are induced.
T1668	5123-5125	IN	denotes	In
T1670	5126-5128	DT	denotes	an
T1672	5129-5132	NN	denotes	MTF
T1674	5132-5133	HYPH	denotes	-
T1675	5133-5134	CD	denotes	1
T1676	5134-5135	HYPH	denotes	-
T1673	5135-5146	JJ	denotes	independent
T1677	5147-5160	NN	denotes	transcriptome
T1671	5161-5169	NN	denotes	response
T1678	5169-5171	,	denotes	,
T1679	5171-5178	JJ	denotes	several
T1680	5179-5184	NNS	denotes	genes
T1681	5185-5193	VBN	denotes	involved
T1682	5194-5196	IN	denotes	in
T1683	5197-5208	NN	denotes	glutathione
T1684	5209-5219	NN	denotes	metabolism
T1685	5220-5223	VBP	denotes	are
T1669	5224-5231	VBN	denotes	induced
T1686	5231-5232	.	denotes	.
T1687	5232-5383	sentence	denotes	Further studies confirmed a dual anti-cadmium defense, one via glutathione and another one via MTF-1 and its target genes, including metallothioneins.
T1688	5233-5240	JJ	denotes	Further
T1689	5241-5248	NNS	denotes	studies
T1690	5249-5258	VBD	denotes	confirmed
T1691	5259-5260	DT	denotes	a
T1693	5261-5265	JJ	denotes	dual
T1694	5266-5278	JJ	denotes	anti-cadmium
T1692	5279-5286	NN	denotes	defense
T1695	5286-5288	,	denotes	,
T1696	5288-5291	CD	denotes	one
T1697	5292-5295	IN	denotes	via
T1698	5296-5307	NN	denotes	glutathione
T1699	5308-5311	CC	denotes	and
T1700	5312-5319	DT	denotes	another
T1701	5320-5323	CD	denotes	one
T1702	5324-5327	IN	denotes	via
T1703	5328-5331	NN	denotes	MTF
T1704	5331-5332	HYPH	denotes	-
T1705	5332-5333	CD	denotes	1
T1706	5334-5337	CC	denotes	and
T1707	5338-5341	PRP$	denotes	its
T1709	5342-5348	NN	denotes	target
T1708	5349-5354	NNS	denotes	genes
T1710	5354-5356	,	denotes	,
T1711	5356-5365	VBG	denotes	including
T1712	5366-5382	NNS	denotes	metallothioneins
T1713	5382-5383	.	denotes	.
T1758	5408-5418	NN	denotes	Generation
T1759	5419-5421	IN	denotes	of
T1760	5422-5426	NN	denotes	Mtf1
T1762	5427-5438	JJ	denotes	conditional
T1763	5439-5447	NN	denotes	knockout
T1761	5448-5452	NNS	denotes	mice
T1764	5453-5456	CC	denotes	and
T1765	5457-5462	NN	denotes	liver
T1767	5462-5463	HYPH	denotes	-
T1766	5463-5471	JJ	denotes	specific
T1768	5472-5480	NN	denotes	deletion
T1769	5480-5585	sentence	denotes	Mtf1 conditional knockout mice were generated in collaboration with Dr Michael Leviten (San Carlos, CA).
T1770	5481-5485	NN	denotes	Mtf1
T1772	5486-5497	JJ	denotes	conditional
T1773	5498-5506	NN	denotes	knockout
T1771	5507-5511	NNS	denotes	mice
T1775	5512-5516	VBD	denotes	were
T1774	5517-5526	VBN	denotes	generated
T1776	5527-5529	IN	denotes	in
T1777	5530-5543	NN	denotes	collaboration
T1778	5544-5548	IN	denotes	with
T1779	5549-5551	NNP	denotes	Dr
T1781	5552-5559	NNP	denotes	Michael
T1780	5560-5567	NNP	denotes	Leviten
T1782	5568-5569	-LRB-	denotes	(
T1784	5569-5572	NNP	denotes	San
T1783	5573-5579	NNP	denotes	Carlos
T1785	5579-5581	,	denotes	,
T1786	5581-5583	NNP	denotes	CA
T1787	5583-5584	-RRB-	denotes	)
T1788	5584-5585	.	denotes	.
T1789	5585-5734	sentence	denotes	Two genomic clones containing exons 3 to 6 of Mtf1 were used to construct a gene targeting vector for homologous recombination (Supplementary Data).
T1790	5586-5589	CD	denotes	Two
T1792	5590-5597	JJ	denotes	genomic
T1791	5598-5604	NNS	denotes	clones
T1794	5605-5615	VBG	denotes	containing
T1795	5616-5621	NNS	denotes	exons
T1796	5622-5623	CD	denotes	3
T1797	5624-5626	IN	denotes	to
T1798	5627-5628	CD	denotes	6
T1799	5629-5631	IN	denotes	of
T1800	5632-5636	NN	denotes	Mtf1
T1801	5637-5641	VBD	denotes	were
T1793	5642-5646	VBN	denotes	used
T1802	5647-5649	TO	denotes	to
T1803	5650-5659	VB	denotes	construct
T1804	5660-5661	DT	denotes	a
T1806	5662-5666	NN	denotes	gene
T1807	5667-5676	NN	denotes	targeting
T1805	5677-5683	NN	denotes	vector
T1808	5684-5687	IN	denotes	for
T1809	5688-5698	JJ	denotes	homologous
T1810	5699-5712	NN	denotes	recombination
T1811	5713-5714	-LRB-	denotes	(
T1813	5714-5727	JJ	denotes	Supplementary
T1812	5728-5732	NNS	denotes	Data
T1814	5732-5733	-RRB-	denotes	)
T1815	5733-5734	.	denotes	.
T1816	5734-5917	sentence	denotes	A neomycin resistance cassette (PGK-neo) flanked by two loxP sites was cloned into the SacI site 5′ of exon 3 of Mtf1, the third loxP site was cloned into the ScaI site 3′ of exon 4.
T1817	5735-5736	DT	denotes	A
T1819	5737-5745	NN	denotes	neomycin
T1820	5746-5756	NN	denotes	resistance
T1818	5757-5765	NN	denotes	cassette
T1822	5766-5767	-LRB-	denotes	(
T1823	5767-5770	NN	denotes	PGK
T1825	5770-5771	HYPH	denotes	-
T1824	5771-5774	NN	denotes	neo
T1826	5774-5775	-RRB-	denotes	)
T1827	5776-5783	VBN	denotes	flanked
T1828	5784-5786	IN	denotes	by
T1829	5787-5790	CD	denotes	two
T1831	5791-5795	NN	denotes	loxP
T1830	5796-5801	NNS	denotes	sites
T1832	5802-5805	VBD	denotes	was
T1821	5806-5812	VBN	denotes	cloned
T1834	5813-5817	IN	denotes	into
T1835	5818-5821	DT	denotes	the
T1837	5822-5826	NN	denotes	SacI
T1836	5827-5831	NN	denotes	site
T1838	5832-5833	CD	denotes	5
T1839	5833-5834	SYM	denotes	′
T1840	5835-5837	IN	denotes	of
T1841	5838-5842	NN	denotes	exon
T1842	5843-5844	CD	denotes	3
T1843	5845-5847	IN	denotes	of
T1844	5848-5852	NN	denotes	Mtf1
T1845	5852-5854	,	denotes	,
T1846	5854-5857	DT	denotes	the
T1848	5858-5863	JJ	denotes	third
T1849	5864-5868	NN	denotes	loxP
T1847	5869-5873	NN	denotes	site
T1850	5874-5877	VBD	denotes	was
T1833	5878-5884	VBN	denotes	cloned
T1851	5885-5889	IN	denotes	into
T1852	5890-5893	DT	denotes	the
T1854	5894-5898	NN	denotes	ScaI
T1853	5899-5903	NN	denotes	site
T1855	5904-5905	CD	denotes	3
T1856	5905-5906	SYM	denotes	′
T1857	5907-5909	IN	denotes	of
T1858	5910-5914	NN	denotes	exon
T1859	5915-5916	CD	denotes	4
T1860	5916-5917	.	denotes	.
T1861	5917-5994	sentence	denotes	A thymidine kinase (TK) cassette was inserted in the HpaI site 3′ of exon 6.
T1862	5918-5919	DT	denotes	A
T1864	5920-5929	NN	denotes	thymidine
T1865	5930-5936	NN	denotes	kinase
T1866	5937-5938	-LRB-	denotes	(
T1867	5938-5940	NN	denotes	TK
T1868	5940-5941	-RRB-	denotes	)
T1863	5942-5950	NN	denotes	cassette
T1870	5951-5954	VBD	denotes	was
T1869	5955-5963	VBN	denotes	inserted
T1871	5964-5966	IN	denotes	in
T1872	5967-5970	DT	denotes	the
T1874	5971-5975	NN	denotes	HpaI
T1873	5976-5980	NN	denotes	site
T1875	5981-5982	CD	denotes	3
T1876	5982-5983	SYM	denotes	′
T1877	5984-5986	IN	denotes	of
T1878	5987-5991	NN	denotes	exon
T1879	5992-5993	CD	denotes	6
T1880	5993-5994	.	denotes	.
T1881	5994-6187	sentence	denotes	129 ES cells were electroporated with the linearized targeting vector, selected in the presence of G418 and FIAU, and screened for correct integration events by PCR and Southern blot analysis.
T1882	5995-5998	CD	denotes	129
T1884	5999-6001	NN	denotes	ES
T1883	6002-6007	NNS	denotes	cells
T1886	6008-6012	VBD	denotes	were
T1885	6013-6027	VBN	denotes	electroporated
T1887	6028-6032	IN	denotes	with
T1888	6033-6036	DT	denotes	the
T1890	6037-6047	VBN	denotes	linearized
T1891	6048-6057	NN	denotes	targeting
T1889	6058-6064	NN	denotes	vector
T1892	6064-6066	,	denotes	,
T1893	6066-6074	VBN	denotes	selected
T1894	6075-6077	IN	denotes	in
T1895	6078-6081	DT	denotes	the
T1896	6082-6090	NN	denotes	presence
T1897	6091-6093	IN	denotes	of
T1898	6094-6098	NN	denotes	G418
T1899	6099-6102	CC	denotes	and
T1900	6103-6107	NN	denotes	FIAU
T1901	6107-6109	,	denotes	,
T1902	6109-6112	CC	denotes	and
T1903	6113-6121	VBN	denotes	screened
T1904	6122-6125	IN	denotes	for
T1905	6126-6133	JJ	denotes	correct
T1907	6134-6145	NN	denotes	integration
T1906	6146-6152	NNS	denotes	events
T1908	6153-6155	IN	denotes	by
T1909	6156-6159	NN	denotes	PCR
T1911	6160-6163	CC	denotes	and
T1912	6164-6172	NNP	denotes	Southern
T1913	6173-6177	NN	denotes	blot
T1910	6178-6186	NN	denotes	analysis
T1914	6186-6187	.	denotes	.
T1915	6187-6410	sentence	denotes	Transient expression of Cre recombinase led to removal of the PGK-neo cassette, and mice carrying the modified Mtf1loxP allele were generated by injection of positive clones into C57Bl/6 blastocysts and subsequent crosses.
T1916	6188-6197	JJ	denotes	Transient
T1917	6198-6208	NN	denotes	expression
T1919	6209-6211	IN	denotes	of
T1920	6212-6215	NN	denotes	Cre
T1921	6216-6227	NN	denotes	recombinase
T1918	6228-6231	VBD	denotes	led
T1922	6232-6234	IN	denotes	to
T1923	6235-6242	NN	denotes	removal
T1924	6243-6245	IN	denotes	of
T1925	6246-6249	DT	denotes	the
T1927	6250-6253	NN	denotes	PGK
T1929	6253-6254	HYPH	denotes	-
T1928	6254-6257	NN	denotes	neo
T1926	6258-6266	NN	denotes	cassette
T1930	6266-6268	,	denotes	,
T1931	6268-6271	CC	denotes	and
T1932	6272-6276	NNS	denotes	mice
T1934	6277-6285	VBG	denotes	carrying
T1935	6286-6289	DT	denotes	the
T1937	6290-6298	VBN	denotes	modified
T1938	6299-6307	NN	denotes	Mtf1loxP
T1936	6308-6314	NN	denotes	allele
T1939	6315-6319	VBD	denotes	were
T1933	6320-6329	VBN	denotes	generated
T1940	6330-6332	IN	denotes	by
T1941	6333-6342	NN	denotes	injection
T1942	6343-6345	IN	denotes	of
T1943	6346-6354	JJ	denotes	positive
T1944	6355-6361	NNS	denotes	clones
T1945	6362-6366	IN	denotes	into
T1946	6367-6372	NN	denotes	C57Bl
T1948	6372-6373	HYPH	denotes	/
T1949	6373-6374	CD	denotes	6
T1947	6375-6386	NNS	denotes	blastocysts
T1950	6387-6390	CC	denotes	and
T1951	6391-6401	JJ	denotes	subsequent
T1952	6402-6409	NNS	denotes	crosses
T1953	6409-6410	.	denotes	.
T1954	6410-6619	sentence	denotes	Homozygous conditional knockout animals (Mtf1loxP/loxP) were crossed with the Cre recombinase transgenic line Mx-cre (a gift from Prof. Michel Aguet) to obtain an inducible, liver-specific Mtf1 knockout line.
T1955	6411-6421	JJ	denotes	Homozygous
T1957	6422-6433	JJ	denotes	conditional
T1958	6434-6442	NN	denotes	knockout
T1956	6443-6450	NNS	denotes	animals
T1960	6451-6452	-LRB-	denotes	(
T1961	6452-6460	NN	denotes	Mtf1loxP
T1963	6460-6461	HYPH	denotes	/
T1962	6461-6465	NN	denotes	loxP
T1964	6465-6466	-RRB-	denotes	)
T1965	6467-6471	VBD	denotes	were
T1959	6472-6479	VBN	denotes	crossed
T1966	6480-6484	IN	denotes	with
T1967	6485-6488	DT	denotes	the
T2074	6489-6492	NN	denotes	Cre
T2075	6493-6504	NN	denotes	recombinase
T2076	6505-6515	JJ	denotes	transgenic
T1968	6516-6520	NN	denotes	line
T2077	6521-6523	NN	denotes	Mx
T2079	6523-6524	HYPH	denotes	-
T2078	6524-6527	NN	denotes	cre
T2080	6528-6529	-LRB-	denotes	(
T2082	6529-6530	DT	denotes	a
T2081	6531-6535	NN	denotes	gift
T2083	6536-6540	IN	denotes	from
T2084	6541-6546	NNP	denotes	Prof.
T2086	6547-6553	NNP	denotes	Michel
T2085	6554-6559	NNP	denotes	Aguet
T2087	6559-6560	-RRB-	denotes	)
T2088	6561-6563	TO	denotes	to
T2089	6564-6570	VB	denotes	obtain
T2090	6571-6573	DT	denotes	an
T2092	6574-6583	JJ	denotes	inducible
T2093	6583-6585	,	denotes	,
T2094	6585-6590	NN	denotes	liver
T2096	6590-6591	HYPH	denotes	-
T2095	6591-6599	JJ	denotes	specific
T2097	6600-6604	NN	denotes	Mtf1
T2098	6605-6613	NN	denotes	knockout
T2091	6614-6618	NN	denotes	line
T2099	6618-6619	.	denotes	.
T2100	6619-6873	sentence	denotes	The mice were genotyped by PCR using the following primers (Microsynth): Cre recombinase: 5′-CTATCCAGCAACATTTGGGCCAGC-3′; 5′-CCAGGTTACGGATATAGTTCATGAC-3′, Mtf1loxP or wild-type allele: 5′-CACACCCAGTTTGTGTATGTCTTC-3′; 5′-CAGTCACAAGCAAATTACCAAACACTGCC-3′.
T2101	6620-6623	DT	denotes	The
T2102	6624-6628	NNS	denotes	mice
T2104	6629-6633	VBD	denotes	were
T2103	6634-6643	VBN	denotes	genotyped
T2105	6644-6646	IN	denotes	by
T2106	6647-6650	NN	denotes	PCR
T2107	6651-6656	VBG	denotes	using
T2108	6657-6660	DT	denotes	the
T2110	6661-6670	VBG	denotes	following
T2109	6671-6678	NNS	denotes	primers
T2111	6679-6680	-LRB-	denotes	(
T2112	6680-6690	NNP	denotes	Microsynth
T2113	6690-6691	-RRB-	denotes	)
T2114	6691-6693	:	denotes	:
T2115	6693-6696	NN	denotes	Cre
T2116	6697-6708	NN	denotes	recombinase
T2118	6708-6710	:	denotes	:
T2119	6710-6711	CD	denotes	5
T2121	6711-6712	SYM	denotes	′
T2122	6712-6713	HYPH	denotes	-
T2120	6713-6737	NN	denotes	CTATCCAGCAACATTTGGGCCAGC
T2123	6737-6738	HYPH	denotes	-
T2124	6738-6739	CD	denotes	3
T2125	6739-6740	SYM	denotes	′
T2126	6740-6741	:	denotes	;
T2127	6742-6743	CD	denotes	5
T2128	6743-6744	SYM	denotes	′
T2129	6744-6745	HYPH	denotes	-
T2117	6745-6770	NN	denotes	CCAGGTTACGGATATAGTTCATGAC
T2131	6770-6771	HYPH	denotes	-
T2132	6771-6772	CD	denotes	3
T2133	6772-6773	SYM	denotes	′
T2134	6773-6775	,	denotes	,
T2135	6775-6783	NN	denotes	Mtf1loxP
T2136	6784-6786	CC	denotes	or
T2137	6787-6791	JJ	denotes	wild
T2139	6791-6792	HYPH	denotes	-
T2138	6792-6796	NN	denotes	type
T2140	6797-6803	NN	denotes	allele
T2141	6803-6805	:	denotes	:
T2142	6805-6806	CD	denotes	5
T2144	6806-6807	SYM	denotes	′
T2145	6807-6808	HYPH	denotes	-
T2143	6808-6832	NN	denotes	CACACCCAGTTTGTGTATGTCTTC
T2146	6832-6833	HYPH	denotes	-
T2147	6833-6834	CD	denotes	3
T2148	6834-6835	SYM	denotes	′
T2149	6835-6836	:	denotes	;
T2150	6837-6838	CD	denotes	5
T2151	6838-6839	SYM	denotes	′
T2152	6839-6840	HYPH	denotes	-
T2130	6840-6869	NN	denotes	CAGTCACAAGCAAATTACCAAACACTGCC
T2153	6869-6870	HYPH	denotes	-
T2154	6870-6871	CD	denotes	3
T2155	6871-6872	SYM	denotes	′
T2156	6872-6873	.	denotes	.
T2173	6875-6881	NN	denotes	Animal
T2174	6882-6891	NN	denotes	treatment
T2175	6891-7289	sentence	denotes	At 8 weeks of age, male Mtf1loxP/loxP mice harboring the Mx-cre transgene (Mtf1loxP/loxP Mx-cre, abbr.: Mtf1Mx-cre) and control littermates without transgene (Mtf1loxP/loxP, abbr.: Mtf1loxP) received four intraperitoneal injections each of 300 µg synthetic double-stranded RNA polyinosinic–polycytidylic acid [pI–pC; Sigma; in a volume of 60 µl phosphate-buffered saline (PBS)] at 3 day intervals.
T2176	6892-6894	IN	denotes	At
T2178	6895-6896	CD	denotes	8
T2179	6897-6902	NNS	denotes	weeks
T2180	6903-6905	IN	denotes	of
T2181	6906-6909	NN	denotes	age
T2182	6909-6911	,	denotes	,
T2183	6911-6915	JJ	denotes	male
T2185	6916-6924	NN	denotes	Mtf1loxP
T2187	6924-6925	HYPH	denotes	/
T2186	6925-6929	NN	denotes	loxP
T2184	6930-6934	NNS	denotes	mice
T2188	6935-6944	VBG	denotes	harboring
T2189	6945-6948	DT	denotes	the
T2191	6949-6951	NN	denotes	Mx
T2193	6951-6952	HYPH	denotes	-
T2192	6952-6955	NN	denotes	cre
T2190	6956-6965	NN	denotes	transgene
T2194	6966-6967	-LRB-	denotes	(
T2195	6967-6975	NN	denotes	Mtf1loxP
T2197	6975-6976	HYPH	denotes	/
T2198	6976-6980	NN	denotes	loxP
T2199	6981-6983	NN	denotes	Mx
T2200	6983-6984	HYPH	denotes	-
T2196	6984-6987	NN	denotes	cre
T2201	6987-6989	,	denotes	,
T2202	6989-6994	RB	denotes	abbr.
T2204	6994-6996	:	denotes	:
T2205	6996-7002	NN	denotes	Mtf1Mx
T2206	7002-7003	HYPH	denotes	-
T2203	7003-7006	NN	denotes	cre
T2207	7006-7007	-RRB-	denotes	)
T2208	7008-7011	CC	denotes	and
T2209	7012-7019	NN	denotes	control
T2210	7020-7031	NNS	denotes	littermates
T2211	7032-7039	IN	denotes	without
T2212	7040-7049	NN	denotes	transgene
T2213	7050-7051	-LRB-	denotes	(
T2214	7051-7059	NN	denotes	Mtf1loxP
T2216	7059-7060	HYPH	denotes	/
T2215	7060-7064	NN	denotes	loxP
T2217	7064-7066	,	denotes	,
T2218	7066-7071	NN	denotes	abbr.
T2220	7071-7073	:	denotes	:
T2219	7073-7081	NN	denotes	Mtf1loxP
T2221	7081-7082	-RRB-	denotes	)
T2177	7083-7091	VBD	denotes	received
T2222	7092-7096	CD	denotes	four
T2224	7097-7112	JJ	denotes	intraperitoneal
T2223	7113-7123	NNS	denotes	injections
T2225	7124-7128	RB	denotes	each
T2226	7129-7131	IN	denotes	of
T2227	7132-7135	CD	denotes	300
T2228	7136-7138	NN	denotes	µg
T2230	7139-7148	JJ	denotes	synthetic
T2231	7149-7155	JJ	denotes	double
T2234	7155-7156	HYPH	denotes	-
T2232	7156-7164	VBN	denotes	stranded
T2235	7165-7168	NN	denotes	RNA
T2236	7169-7181	JJ	denotes	polyinosinic
T2238	7181-7182	HYPH	denotes	–
T2237	7182-7195	JJ	denotes	polycytidylic
T2229	7196-7200	NN	denotes	acid
T2239	7201-7202	-LRB-	denotes	[
T2241	7202-7204	NN	denotes	pI
T2243	7204-7205	HYPH	denotes	–
T2242	7205-7207	NN	denotes	pC
T2244	7207-7208	:	denotes	;
T2240	7209-7214	NNP	denotes	Sigma
T2245	7214-7215	:	denotes	;
T2246	7216-7218	IN	denotes	in
T2247	7219-7220	DT	denotes	a
T2248	7221-7227	NN	denotes	volume
T2249	7228-7230	IN	denotes	of
T2250	7231-7233	CD	denotes	60
T2251	7234-7236	NN	denotes	µl
T2252	7237-7246	NN	denotes	phosphate
T2254	7246-7247	HYPH	denotes	-
T2253	7247-7255	VBN	denotes	buffered
T2255	7256-7262	NN	denotes	saline
T2256	7263-7264	-LRB-	denotes	(
T2257	7264-7267	NN	denotes	PBS
T2258	7267-7268	-RRB-	denotes	)
T2259	7268-7269	-RRB-	denotes	]
T2260	7270-7272	IN	denotes	at
T2261	7273-7274	CD	denotes	3
T2262	7275-7278	NN	denotes	day
T2263	7279-7288	NNS	denotes	intervals
T2264	7288-7289	.	denotes	.
T2265	7289-7430	sentence	denotes	Only in the case of DNA-binding studies with MRE sequences from MTF-1 target gene candidates, the control mice received no pI–pC injections.
T2266	7290-7294	RB	denotes	Only
T2267	7295-7297	IN	denotes	in
T2269	7298-7301	DT	denotes	the
T2270	7302-7306	NN	denotes	case
T2271	7307-7309	IN	denotes	of
T2272	7310-7313	NN	denotes	DNA
T2274	7313-7314	HYPH	denotes	-
T2273	7314-7321	VBG	denotes	binding
T2275	7322-7329	NNS	denotes	studies
T2276	7330-7334	IN	denotes	with
T2277	7335-7338	NN	denotes	MRE
T2278	7339-7348	NNS	denotes	sequences
T2279	7349-7353	IN	denotes	from
T2280	7354-7357	NN	denotes	MTF
T2282	7357-7358	HYPH	denotes	-
T2283	7358-7359	CD	denotes	1
T2284	7360-7366	NN	denotes	target
T2285	7367-7371	NN	denotes	gene
T2281	7372-7382	NNS	denotes	candidates
T2286	7382-7384	,	denotes	,
T2287	7384-7387	DT	denotes	the
T2289	7388-7395	NN	denotes	control
T2288	7396-7400	NNS	denotes	mice
T2268	7401-7409	VBD	denotes	received
T2290	7410-7412	DT	denotes	no
T2292	7413-7415	NN	denotes	pI
T2294	7415-7416	HYPH	denotes	–
T2293	7416-7418	NN	denotes	pC
T2291	7419-7429	NNS	denotes	injections
T2295	7429-7430	.	denotes	.
T2296	7430-7704	sentence	denotes	For experiments with metal treatment, mice received 2 days after the last pI–pC treatment a subcutaneous (s.c.) injection of either 20 µmol/kg body weight CdSO4 (2 mM CdSO4 in H2O; cadmium treatment) or 10 ml/kg body weight H2O (mock treatment) 6 h before sacrificing them.
T2297	7431-7434	IN	denotes	For
T2299	7435-7446	NNS	denotes	experiments
T2300	7447-7451	IN	denotes	with
T2301	7452-7457	NN	denotes	metal
T2302	7458-7467	NN	denotes	treatment
T2303	7467-7469	,	denotes	,
T2304	7469-7473	NNS	denotes	mice
T2298	7474-7482	VBD	denotes	received
T2305	7483-7484	CD	denotes	2
T2306	7485-7489	NNS	denotes	days
T2307	7490-7495	IN	denotes	after
T2308	7496-7499	DT	denotes	the
T2310	7500-7504	JJ	denotes	last
T2311	7505-7507	NN	denotes	pI
T2313	7507-7508	HYPH	denotes	–
T2312	7508-7510	NN	denotes	pC
T2309	7511-7520	NN	denotes	treatment
T2314	7521-7522	DT	denotes	a
T2316	7523-7535	JJ	denotes	subcutaneous
T2318	7536-7537	-LRB-	denotes	(
T2317	7537-7541	JJ	denotes	s.c.
T2319	7541-7542	-RRB-	denotes	)
T2315	7543-7552	NN	denotes	injection
T2320	7553-7555	IN	denotes	of
T2321	7556-7562	CC	denotes	either
T2233	7563-7565	CD	denotes	20
T2323	7566-7570	NN	denotes	µmol
T2324	7570-7571	SYM	denotes	/
T2326	7571-7573	NN	denotes	kg
T2327	7574-7578	NN	denotes	body
T2325	7579-7585	NN	denotes	weight
T2322	7586-7591	NN	denotes	CdSO4
T2328	7592-7593	-LRB-	denotes	(
T2330	7593-7594	CD	denotes	2
T2331	7595-7597	NN	denotes	mM
T2332	7598-7603	NN	denotes	CdSO4
T2333	7604-7606	IN	denotes	in
T2334	7607-7610	NN	denotes	H2O
T2335	7610-7611	:	denotes	;
T2336	7612-7619	NN	denotes	cadmium
T2329	7620-7629	NN	denotes	treatment
T2337	7629-7630	-RRB-	denotes	)
T2338	7631-7633	CC	denotes	or
T2339	7634-7636	CD	denotes	10
T2340	7637-7639	NN	denotes	ml
T2342	7639-7640	SYM	denotes	/
T2344	7640-7642	NN	denotes	kg
T2345	7643-7647	NN	denotes	body
T2343	7648-7654	NN	denotes	weight
T2341	7655-7658	NN	denotes	H2O
T2346	7659-7660	-LRB-	denotes	(
T2348	7660-7664	JJ	denotes	mock
T2347	7665-7674	NN	denotes	treatment
T2349	7674-7675	-RRB-	denotes	)
T2350	7676-7677	CD	denotes	6
T2351	7678-7679	NN	denotes	h
T2352	7680-7686	IN	denotes	before
T2353	7687-7698	VBG	denotes	sacrificing
T2354	7699-7703	PRP	denotes	them
T2355	7703-7704	.	denotes	.
T2531	7706-7716	NN	denotes	Microarray
T2532	7717-7725	NN	denotes	analysis
T2533	7726-7729	CC	denotes	and
T2534	7730-7734	NNS	denotes	data
T2535	7735-7745	NN	denotes	processing
T2536	7745-7971	sentence	denotes	Total RNA was isolated from liver tissue of pI–pC-induced, mock- or cadmium-treated Mtf1Mx-cre and Mtf1loxP mice (n = 3 per genotype and respective treatment; all male) essentially as described by Chomczynski and Sacchi (27).
T2537	7746-7751	JJ	denotes	Total
T2538	7752-7755	NN	denotes	RNA
T2540	7756-7759	VBD	denotes	was
T2539	7760-7768	VBN	denotes	isolated
T2541	7769-7773	IN	denotes	from
T2542	7774-7779	NN	denotes	liver
T2543	7780-7786	NN	denotes	tissue
T2544	7787-7789	IN	denotes	of
T2545	7790-7792	NN	denotes	pI
T2547	7792-7793	HYPH	denotes	–
T2546	7793-7795	NN	denotes	pC
T2549	7795-7796	HYPH	denotes	-
T2548	7796-7803	VBN	denotes	induced
T2551	7803-7805	,	denotes	,
T2552	7805-7809	JJ	denotes	mock
T2554	7809-7810	HYPH	denotes	-
T2555	7811-7813	CC	denotes	or
T2556	7814-7821	NN	denotes	cadmium
T2557	7821-7822	HYPH	denotes	-
T2553	7822-7829	VBN	denotes	treated
T2558	7830-7836	NN	denotes	Mtf1Mx
T2560	7836-7837	HYPH	denotes	-
T2559	7837-7840	NN	denotes	cre
T2561	7841-7844	CC	denotes	and
T2562	7845-7853	NN	denotes	Mtf1loxP
T2550	7854-7858	NNS	denotes	mice
T2563	7859-7860	-LRB-	denotes	(
T2565	7860-7861	NN	denotes	n
T2567	7862-7863	SYM	denotes	=
T2566	7864-7865	CD	denotes	3
T2568	7866-7869	IN	denotes	per
T2569	7870-7878	NN	denotes	genotype
T2570	7879-7882	CC	denotes	and
T2571	7883-7893	JJ	denotes	respective
T2572	7894-7903	NN	denotes	treatment
T2573	7903-7904	:	denotes	;
T2574	7905-7908	DT	denotes	all
T2564	7909-7913	JJ	denotes	male
T2575	7913-7914	-RRB-	denotes	)
T2576	7915-7926	RB	denotes	essentially
T2578	7927-7929	IN	denotes	as
T2577	7930-7939	VBN	denotes	described
T2579	7940-7942	IN	denotes	by
T2580	7943-7954	NNP	denotes	Chomczynski
T2581	7955-7958	CC	denotes	and
T2582	7959-7965	NNP	denotes	Sacchi
T2583	7966-7967	-LRB-	denotes	(
T2584	7967-7969	CD	denotes	27
T2585	7969-7970	-RRB-	denotes	)
T2586	7970-7971	.	denotes	.
T2587	7971-8196	sentence	denotes	Gene expression analysis was performed in the Functional Genomics Center Zurich using GeneChip® Mouse Genome 430 2.0 Arrays (Affymetrix) according to the manufacturer's instructions and the following reagents and conditions.
T2588	7972-7976	NN	denotes	Gene
T2589	7977-7987	NN	denotes	expression
T2590	7988-7996	NN	denotes	analysis
T2592	7997-8000	VBD	denotes	was
T2591	8001-8010	VBN	denotes	performed
T2593	8011-8013	IN	denotes	in
T2594	8014-8017	DT	denotes	the
T2596	8018-8028	NNP	denotes	Functional
T2597	8029-8037	NNP	denotes	Genomics
T2595	8038-8044	NNP	denotes	Center
T2598	8045-8051	NNP	denotes	Zurich
T2599	8052-8057	VBG	denotes	using
T2600	8058-8067	NNP	denotes	GeneChip®
T2602	8068-8073	NNP	denotes	Mouse
T2603	8074-8080	NNP	denotes	Genome
T2604	8081-8084	CD	denotes	430
T2605	8085-8088	CD	denotes	2.0
T2601	8089-8095	NNS	denotes	Arrays
T2606	8096-8097	-LRB-	denotes	(
T2607	8097-8107	NNP	denotes	Affymetrix
T2608	8107-8108	-RRB-	denotes	)
T2609	8109-8118	VBG	denotes	according
T2610	8119-8121	IN	denotes	to
T2611	8122-8125	DT	denotes	the
T2612	8126-8138	NN	denotes	manufacturer
T2614	8138-8140	POS	denotes	's
T2613	8141-8153	NNS	denotes	instructions
T2615	8154-8157	CC	denotes	and
T2616	8158-8161	DT	denotes	the
T2618	8162-8171	VBG	denotes	following
T2617	8172-8180	NNS	denotes	reagents
T2619	8181-8184	CC	denotes	and
T2620	8185-8195	NNS	denotes	conditions
T2621	8195-8196	.	denotes	.
T2622	8196-8307	sentence	denotes	cDNA was synthesized with SuperScript™ Double-Stranded cDNA Synthesis Kit (Invitrogen), using 15 µg total RNA.
T2623	8197-8201	NN	denotes	cDNA
T2625	8202-8205	VBD	denotes	was
T2624	8206-8217	VBN	denotes	synthesized
T2626	8218-8222	IN	denotes	with
T2627	8223-8234	NNP	denotes	SuperScript
T2629	8234-8235	SYM	denotes	™
T2630	8236-8242	JJ	denotes	Double
T2632	8242-8243	HYPH	denotes	-
T2631	8243-8251	VBN	denotes	Stranded
T2633	8252-8256	NN	denotes	cDNA
T2634	8257-8266	NN	denotes	Synthesis
T2628	8267-8270	NN	denotes	Kit
T2635	8271-8272	-LRB-	denotes	(
T2636	8272-8282	NNP	denotes	Invitrogen
T2637	8282-8283	-RRB-	denotes	)
T2638	8283-8285	,	denotes	,
T2639	8285-8290	VBG	denotes	using
T2640	8291-8293	CD	denotes	15
T2641	8294-8296	NN	denotes	µg
T2643	8297-8302	JJ	denotes	total
T2642	8303-8306	NN	denotes	RNA
T2644	8306-8307	.	denotes	.
T2645	8307-8436	sentence	denotes	In vitro transcription was performed with BioArray™ High Yield™ RNA Transcript Labeling Kit (Enzo) and 3.5 to 6 µg of each cDNA.
T2646	8308-8310	FW	denotes	In
T2647	8311-8316	FW	denotes	vitro
T2648	8317-8330	NN	denotes	transcription
T2650	8331-8334	VBD	denotes	was
T2649	8335-8344	VBN	denotes	performed
T2651	8345-8349	IN	denotes	with
T2652	8350-8358	NNP	denotes	BioArray
T2654	8358-8359	SYM	denotes	™
T2655	8360-8364	NNP	denotes	High
T2656	8365-8370	NNP	denotes	Yield
T2657	8370-8371	SYM	denotes	™
T2658	8372-8375	NN	denotes	RNA
T2659	8376-8386	NN	denotes	Transcript
T2660	8387-8395	NN	denotes	Labeling
T2653	8396-8399	NN	denotes	Kit
T2661	8400-8401	-LRB-	denotes	(
T2662	8401-8405	NNP	denotes	Enzo
T2663	8405-8406	-RRB-	denotes	)
T2664	8407-8410	CC	denotes	and
T2665	8411-8414	CD	denotes	3.5
T2667	8415-8417	IN	denotes	to
T2666	8418-8419	CD	denotes	6
T2668	8420-8422	NN	denotes	µg
T2669	8423-8425	IN	denotes	of
T2670	8426-8430	DT	denotes	each
T2671	8431-8435	NN	denotes	cDNA
T2672	8435-8436	.	denotes	.
T2673	8436-8537	sentence	denotes	Clean-up of both cDNA and cRNA samples was done using GeneChip® Sample Clean-up Module (Affymetrix).
T2674	8437-8442	NN	denotes	Clean
T2676	8442-8443	HYPH	denotes	-
T2675	8443-8445	NN	denotes	up
T2678	8446-8448	IN	denotes	of
T2679	8449-8453	CC	denotes	both
T2680	8454-8458	NN	denotes	cDNA
T2682	8459-8462	CC	denotes	and
T2683	8463-8467	NN	denotes	cRNA
T2681	8468-8475	NNS	denotes	samples
T2684	8476-8479	VBD	denotes	was
T2677	8480-8484	VBN	denotes	done
T2685	8485-8490	VBG	denotes	using
T2686	8491-8500	NNP	denotes	GeneChip®
T2688	8501-8507	NNP	denotes	Sample
T2689	8508-8513	NNP	denotes	Clean
T2691	8513-8514	HYPH	denotes	-
T2690	8514-8516	NNP	denotes	up
T2687	8517-8523	NNP	denotes	Module
T2692	8524-8525	-LRB-	denotes	(
T2693	8525-8535	NNP	denotes	Affymetrix
T2694	8535-8536	-RRB-	denotes	)
T2695	8536-8537	.	denotes	.
T2696	8537-8655	sentence	denotes	For the automated washing and staining in the Affymetrix fluidics station 450, the protocol EukGE-Ws2v4_450 was used.
T2697	8538-8541	IN	denotes	For
T2699	8542-8545	DT	denotes	the
T2701	8546-8555	VBN	denotes	automated
T2700	8556-8563	NN	denotes	washing
T2702	8564-8567	CC	denotes	and
T2703	8568-8576	NN	denotes	staining
T2704	8577-8579	IN	denotes	in
T2705	8580-8583	DT	denotes	the
T2707	8584-8594	NNP	denotes	Affymetrix
T2708	8595-8603	NN	denotes	fluidics
T2706	8604-8611	NN	denotes	station
T2709	8612-8615	CD	denotes	450
T2710	8615-8617	,	denotes	,
T2711	8617-8620	DT	denotes	the
T2713	8621-8629	NN	denotes	protocol
T2714	8630-8635	NN	denotes	EukGE
T2715	8635-8636	HYPH	denotes	-
T2712	8636-8645	NN	denotes	Ws2v4_450
T2716	8646-8649	VBD	denotes	was
T2698	8650-8654	VBN	denotes	used
T2717	8654-8655	.	denotes	.
T2718	8655-8722	sentence	denotes	The probe arrays were scanned with the Affymetrix GS 3000 scanner.
T2719	8656-8659	DT	denotes	The
T2721	8660-8665	NN	denotes	probe
T2720	8666-8672	NNS	denotes	arrays
T2723	8673-8677	VBD	denotes	were
T2722	8678-8685	VBN	denotes	scanned
T2724	8686-8690	IN	denotes	with
T2725	8691-8694	DT	denotes	the
T2727	8695-8705	NNP	denotes	Affymetrix
T2728	8706-8708	NNP	denotes	GS
T2729	8709-8713	CD	denotes	3000
T2726	8714-8721	NN	denotes	scanner
T2730	8721-8722	.	denotes	.
T2731	8722-8794	sentence	denotes	Raw data are available at ArrayExpress (, accession number E-MEXP-438).
T2732	8723-8726	JJ	denotes	Raw
T2733	8727-8731	NNS	denotes	data
T2734	8732-8735	VBP	denotes	are
T2735	8736-8745	JJ	denotes	available
T2736	8746-8748	IN	denotes	at
T2737	8749-8761	NNP	denotes	ArrayExpress
T2738	8762-8763	-LRB-	denotes	(
T2740	8763-8765	,	denotes	,
T2741	8765-8774	NN	denotes	accession
T2742	8775-8781	NN	denotes	number
T2743	8782-8783	NN	denotes	E
T2744	8783-8784	HYPH	denotes	-
T2739	8784-8788	NN	denotes	MEXP
T2745	8788-8789	HYPH	denotes	-
T2746	8789-8792	CD	denotes	438
T2747	8792-8793	-RRB-	denotes	)
T2748	8793-8794	.	denotes	.
T2749	8794-8911	sentence	denotes	Data analysis was performed with GeneSpring 6.1 software (Silicon Genetics), applying a significance level P ≤ 0.05.
T2750	8795-8799	NNS	denotes	Data
T2751	8800-8808	NN	denotes	analysis
T2753	8809-8812	VBD	denotes	was
T2752	8813-8822	VBN	denotes	performed
T2754	8823-8827	IN	denotes	with
T2755	8828-8838	NNP	denotes	GeneSpring
T2757	8839-8842	CD	denotes	6.1
T2756	8843-8851	NN	denotes	software
T2758	8852-8853	-LRB-	denotes	(
T2760	8853-8860	NNP	denotes	Silicon
T2759	8861-8869	NNP	denotes	Genetics
T2761	8869-8870	-RRB-	denotes	)
T2762	8870-8872	,	denotes	,
T2763	8872-8880	VBG	denotes	applying
T2764	8881-8882	DT	denotes	a
T2766	8883-8895	NN	denotes	significance
T2765	8896-8901	NN	denotes	level
T2767	8902-8903	NN	denotes	P
T2769	8904-8905	SYM	denotes	≤
T2768	8906-8910	CD	denotes	0.05
T2770	8910-8911	.	denotes	.
T2771	8911-9028	sentence	denotes	Furthermore, multiple testing correction was used in addition to obtain cadmium-responsive, MTF-1-independent genes.
T2772	8912-8923	RB	denotes	Furthermore
T2774	8923-8925	,	denotes	,
T2775	8925-8933	JJ	denotes	multiple
T2777	8934-8941	NN	denotes	testing
T2776	8942-8952	NN	denotes	correction
T2778	8953-8956	VBD	denotes	was
T2773	8957-8961	VBN	denotes	used
T2779	8962-8964	IN	denotes	in
T2780	8965-8973	NN	denotes	addition
T2781	8974-8976	TO	denotes	to
T2782	8977-8983	VB	denotes	obtain
T2783	8984-8991	NN	denotes	cadmium
T2785	8991-8992	HYPH	denotes	-
T2784	8992-9002	JJ	denotes	responsive
T2787	9002-9004	,	denotes	,
T2788	9004-9007	NN	denotes	MTF
T2790	9007-9008	HYPH	denotes	-
T2791	9008-9009	CD	denotes	1
T2792	9009-9010	HYPH	denotes	-
T2789	9010-9021	JJ	denotes	independent
T2786	9022-9027	NNS	denotes	genes
T2793	9027-9028	.	denotes	.
T2794	9028-9217	sentence	denotes	Genes were considered to be differentially expressed if there was at least a 2-fold difference in expression levels of the compared experimental groups (genotype and respective treatment).
T2795	9029-9034	NNS	denotes	Genes
T2797	9035-9039	VBD	denotes	were
T2796	9040-9050	VBN	denotes	considered
T2798	9051-9053	TO	denotes	to
T2800	9054-9056	VB	denotes	be
T2801	9057-9071	RB	denotes	differentially
T2799	9072-9081	VBN	denotes	expressed
T2802	9082-9084	IN	denotes	if
T2804	9085-9090	EX	denotes	there
T2803	9091-9094	VBD	denotes	was
T2805	9095-9097	RB	denotes	at
T2806	9098-9103	RBS	denotes	least
T2808	9104-9105	DT	denotes	a
T2807	9106-9112	JJ	denotes	2-fold
T2809	9113-9123	NN	denotes	difference
T2810	9124-9126	IN	denotes	in
T2811	9127-9137	NN	denotes	expression
T2812	9138-9144	NNS	denotes	levels
T2813	9145-9147	IN	denotes	of
T2814	9148-9151	DT	denotes	the
T2816	9152-9160	VBN	denotes	compared
T2817	9161-9173	JJ	denotes	experimental
T2815	9174-9180	NNS	denotes	groups
T2818	9181-9182	-LRB-	denotes	(
T2819	9182-9190	NN	denotes	genotype
T2820	9191-9194	CC	denotes	and
T2821	9195-9205	JJ	denotes	respective
T2822	9206-9215	NN	denotes	treatment
T2823	9215-9216	-RRB-	denotes	)
T2824	9216-9217	.	denotes	.
T2825	9217-9402	sentence	denotes	The result was considered reliable if signal values for the respective gene were scored ‘present’ at least for all mice in one experimental group or for two mice in each of two groups.
T2826	9218-9221	DT	denotes	The
T2827	9222-9228	NN	denotes	result
T2829	9229-9232	VBD	denotes	was
T2828	9233-9243	VBN	denotes	considered
T2830	9244-9252	JJ	denotes	reliable
T2831	9253-9255	IN	denotes	if
T2833	9256-9262	NN	denotes	signal
T2834	9263-9269	NNS	denotes	values
T2835	9270-9273	IN	denotes	for
T2836	9274-9277	DT	denotes	the
T2838	9278-9288	JJ	denotes	respective
T2837	9289-9293	NN	denotes	gene
T2839	9294-9298	VBD	denotes	were
T2832	9299-9305	VBN	denotes	scored
T2840	9306-9307	``	denotes	‘
T2841	9307-9314	JJ	denotes	present
T2842	9314-9315	''	denotes	’
T2843	9316-9318	RB	denotes	at
T2844	9319-9324	RBS	denotes	least
T2845	9325-9328	IN	denotes	for
T2846	9329-9332	DT	denotes	all
T2847	9333-9337	NNS	denotes	mice
T2848	9338-9340	IN	denotes	in
T2849	9341-9344	CD	denotes	one
T2851	9345-9357	JJ	denotes	experimental
T2850	9358-9363	NN	denotes	group
T2852	9364-9366	CC	denotes	or
T2853	9367-9370	IN	denotes	for
T2854	9371-9374	CD	denotes	two
T2855	9375-9379	NNS	denotes	mice
T2856	9380-9382	IN	denotes	in
T2857	9383-9387	DT	denotes	each
T2858	9388-9390	IN	denotes	of
T2859	9391-9394	CD	denotes	two
T2860	9395-9401	NNS	denotes	groups
T2861	9401-9402	.	denotes	.
T2862	9402-9665	sentence	denotes	To screen for the presence of the MRE core consensus sequence TGCRCNC in the promoter region, the upstream sequences of the respective genes were obtained from the University of California Santa Cruz (UCSC) Genome Browser Database (; October/November 2004) (28).
T2863	9403-9405	TO	denotes	To
T2864	9406-9412	VB	denotes	screen
T2866	9413-9416	IN	denotes	for
T2867	9417-9420	DT	denotes	the
T2868	9421-9429	NN	denotes	presence
T2869	9430-9432	IN	denotes	of
T2870	9433-9436	DT	denotes	the
T2872	9437-9440	NN	denotes	MRE
T2873	9441-9445	NN	denotes	core
T2874	9446-9455	NN	denotes	consensus
T2871	9456-9464	NN	denotes	sequence
T2875	9465-9472	NN	denotes	TGCRCNC
T2876	9473-9475	IN	denotes	in
T2877	9476-9479	DT	denotes	the
T2879	9480-9488	NN	denotes	promoter
T2878	9489-9495	NN	denotes	region
T2880	9495-9497	,	denotes	,
T2881	9497-9500	DT	denotes	the
T2883	9501-9509	JJ	denotes	upstream
T2882	9510-9519	NNS	denotes	sequences
T2884	9520-9522	IN	denotes	of
T2885	9523-9526	DT	denotes	the
T2887	9527-9537	JJ	denotes	respective
T2886	9538-9543	NNS	denotes	genes
T2888	9544-9548	VBD	denotes	were
T2865	9549-9557	VBN	denotes	obtained
T2889	9558-9562	IN	denotes	from
T2890	9563-9566	DT	denotes	the
T2892	9567-9577	NNP	denotes	University
T2893	9578-9580	IN	denotes	of
T2894	9581-9591	NNP	denotes	California
T2896	9592-9597	NNP	denotes	Santa
T2895	9598-9602	NNP	denotes	Cruz
T2897	9603-9604	-LRB-	denotes	(
T2898	9604-9608	NNP	denotes	UCSC
T2899	9608-9609	-RRB-	denotes	)
T2900	9610-9616	NNP	denotes	Genome
T2901	9617-9624	NNP	denotes	Browser
T2891	9625-9633	NNP	denotes	Database
T2902	9634-9635	-LRB-	denotes	(
T2904	9635-9636	:	denotes	;
T2905	9637-9644	NNP	denotes	October
T2906	9644-9645	SYM	denotes	/
T2903	9645-9653	NNP	denotes	November
T2907	9654-9658	CD	denotes	2004
T2908	9658-9659	-RRB-	denotes	)
T2909	9660-9661	-LRB-	denotes	(
T2910	9661-9663	CD	denotes	28
T2911	9663-9664	-RRB-	denotes	)
T2912	9664-9665	.	denotes	.
T2932	9667-9669	NN	denotes	RT
T2934	9669-9670	HYPH	denotes	–
T2933	9670-9673	NN	denotes	PCR
T2935	9673-9872	sentence	denotes	All RT–PCRs were performed with QIAGEN® OneStep RT–PCR Kit (QIAGEN) according to the manufacturer's instructions, using 150–200 ng DNase I-digested total RNA (RNA isolation see microarray analysis).
T2936	9674-9677	DT	denotes	All
T2938	9678-9680	NN	denotes	RT
T2939	9680-9681	HYPH	denotes	–
T2937	9681-9685	NNS	denotes	PCRs
T2941	9686-9690	VBD	denotes	were
T2940	9691-9700	VBN	denotes	performed
T2942	9701-9705	IN	denotes	with
T2943	9706-9713	NNP	denotes	QIAGEN®
T2945	9714-9721	NNP	denotes	OneStep
T2946	9722-9724	NN	denotes	RT
T2948	9724-9725	HYPH	denotes	–
T2947	9725-9728	NN	denotes	PCR
T2944	9729-9732	NN	denotes	Kit
T2949	9733-9734	-LRB-	denotes	(
T2950	9734-9740	NNP	denotes	QIAGEN
T2951	9740-9741	-RRB-	denotes	)
T2952	9742-9751	VBG	denotes	according
T2953	9752-9754	IN	denotes	to
T2954	9755-9758	DT	denotes	the
T2955	9759-9771	NN	denotes	manufacturer
T2957	9771-9773	POS	denotes	's
T2956	9774-9786	NNS	denotes	instructions
T2958	9786-9788	,	denotes	,
T2959	9788-9793	VBG	denotes	using
T2960	9794-9797	CD	denotes	150
T2962	9797-9798	SYM	denotes	–
T2961	9798-9801	CD	denotes	200
T2963	9802-9804	NN	denotes	ng
T2965	9805-9810	NN	denotes	DNase
T2966	9811-9812	NN	denotes	I
T2968	9812-9813	HYPH	denotes	-
T2967	9813-9821	VBN	denotes	digested
T2969	9822-9827	JJ	denotes	total
T2964	9828-9831	NN	denotes	RNA
T2970	9832-9833	-LRB-	denotes	(
T2972	9833-9836	NN	denotes	RNA
T2971	9837-9846	NN	denotes	isolation
T2973	9847-9850	VBP	denotes	see
T2974	9851-9861	NN	denotes	microarray
T2975	9862-9870	NN	denotes	analysis
T2976	9870-9871	-RRB-	denotes	)
T2977	9871-9872	.	denotes	.
T2978	9872-10376	sentence	denotes	The reactions were carried out using the following primers: Csrp1: 5′-TTCCGATGTGCCAAGTGTGGC-3′; 5′-AGTAGAGAGTGGACATTCAGC-3′, hypoxanthin-guanin-phosphoribosyltransferase (Hprt): 5′-GCTGGTGAAAAGGACCTCTCG-3′; 5′-CCACAGGACTAGGACACCTGC-3′, Mtf1: 5′-GTGACTTTTGAGACTGTACTGAGTG-3′; 5′-CATGCCAAGAAACATTGAAGGTG-3′, Ndrg1: 5′-AGATACACAACAACGTGGAGG-3′; 5′-TGTGCGAGCGGCTTCGGGGGC-3′, Sepw1: 5′-TAGAGGCAGGGTCCTGAAAGC-3′; 5′-ACACCTGGAAACATGGCTGCC-3′, Slc39a10: 5′-GCTGTGGCTGGTAGTAAAAGC-3′; 5′-GTGGCATGGGATGTAAACAGC-3′.
T2979	9873-9876	DT	denotes	The
T2980	9877-9886	NNS	denotes	reactions
T2982	9887-9891	VBD	denotes	were
T2981	9892-9899	VBN	denotes	carried
T2983	9900-9903	RP	denotes	out
T2984	9904-9909	VBG	denotes	using
T2985	9910-9913	DT	denotes	the
T2987	9914-9923	VBG	denotes	following
T2986	9924-9931	NNS	denotes	primers
T2988	9931-9933	:	denotes	:
T2989	9933-9938	NN	denotes	Csrp1
T2990	9938-9940	:	denotes	:
T2991	9940-9941	CD	denotes	5
T2992	9941-9942	SYM	denotes	′
T2993	9942-9943	HYPH	denotes	-
T2931	9943-9964	NN	denotes	TTCCGATGTGCCAAGTGTGGC
T2994	9964-9965	HYPH	denotes	-
T2995	9965-9966	CD	denotes	3
T2996	9966-9967	SYM	denotes	′
T2997	9967-9968	:	denotes	;
T2998	9969-9970	CD	denotes	5
T3000	9970-9971	SYM	denotes	′
T3001	9971-9972	HYPH	denotes	-
T2999	9972-9993	NN	denotes	AGTAGAGAGTGGACATTCAGC
T3002	9993-9994	HYPH	denotes	-
T3003	9994-9995	CD	denotes	3
T3004	9995-9996	SYM	denotes	′
T3005	9996-9998	,	denotes	,
T3006	9998-10009	NN	denotes	hypoxanthin
T3008	10009-10010	HYPH	denotes	-
T3009	10010-10016	NN	denotes	guanin
T3010	10016-10017	HYPH	denotes	-
T3007	10017-10042	NN	denotes	phosphoribosyltransferase
T3011	10043-10044	-LRB-	denotes	(
T3012	10044-10048	NN	denotes	Hprt
T3013	10048-10049	-RRB-	denotes	)
T3014	10049-10051	:	denotes	:
T3015	10051-10052	CD	denotes	5
T3017	10052-10053	SYM	denotes	′
T3018	10053-10054	HYPH	denotes	-
T3016	10054-10075	NN	denotes	GCTGGTGAAAAGGACCTCTCG
T3019	10075-10076	HYPH	denotes	-
T3020	10076-10077	CD	denotes	3
T3021	10077-10078	SYM	denotes	′
T3022	10078-10079	:	denotes	;
T3023	10080-10081	CD	denotes	5
T3025	10081-10082	SYM	denotes	′
T3026	10082-10083	HYPH	denotes	-
T3024	10083-10104	NN	denotes	CCACAGGACTAGGACACCTGC
T3027	10104-10105	HYPH	denotes	-
T3028	10105-10106	CD	denotes	3
T3029	10106-10107	SYM	denotes	′
T3030	10107-10109	,	denotes	,
T3031	10109-10113	NN	denotes	Mtf1
T3032	10113-10115	:	denotes	:
T3033	10115-10116	CD	denotes	5
T3035	10116-10117	SYM	denotes	′
T3036	10117-10118	HYPH	denotes	-
T3034	10118-10143	NN	denotes	GTGACTTTTGAGACTGTACTGAGTG
T3037	10143-10144	HYPH	denotes	-
T3038	10144-10145	CD	denotes	3
T3039	10145-10146	SYM	denotes	′
T3040	10146-10147	:	denotes	;
T3041	10148-10149	CD	denotes	5
T3043	10149-10150	SYM	denotes	′
T3044	10150-10151	HYPH	denotes	-
T3042	10151-10174	NN	denotes	CATGCCAAGAAACATTGAAGGTG
T3045	10174-10175	HYPH	denotes	-
T3046	10175-10176	CD	denotes	3
T3047	10176-10177	SYM	denotes	′
T3048	10177-10179	,	denotes	,
T3049	10179-10184	NN	denotes	Ndrg1
T3050	10184-10186	:	denotes	:
T3051	10186-10187	CD	denotes	5
T3053	10187-10188	SYM	denotes	′
T3054	10188-10189	HYPH	denotes	-
T3052	10189-10210	NN	denotes	AGATACACAACAACGTGGAGG
T3055	10210-10211	HYPH	denotes	-
T3056	10211-10212	CD	denotes	3
T3057	10212-10213	SYM	denotes	′
T3058	10213-10214	:	denotes	;
T3059	10215-10216	CD	denotes	5
T3061	10216-10217	SYM	denotes	′
T3062	10217-10218	HYPH	denotes	-
T3060	10218-10239	NN	denotes	TGTGCGAGCGGCTTCGGGGGC
T3063	10239-10240	HYPH	denotes	-
T3064	10240-10241	CD	denotes	3
T3065	10241-10242	SYM	denotes	′
T3066	10242-10244	,	denotes	,
T3067	10244-10249	NN	denotes	Sepw1
T3068	10249-10251	:	denotes	:
T3069	10251-10252	CD	denotes	5
T3071	10252-10253	SYM	denotes	′
T3072	10253-10254	HYPH	denotes	-
T3070	10254-10275	NN	denotes	TAGAGGCAGGGTCCTGAAAGC
T3073	10275-10276	HYPH	denotes	-
T3074	10276-10277	CD	denotes	3
T3075	10277-10278	SYM	denotes	′
T3076	10278-10279	:	denotes	;
T3077	10280-10281	CD	denotes	5
T3079	10281-10282	SYM	denotes	′
T3080	10282-10283	HYPH	denotes	-
T3078	10283-10304	NN	denotes	ACACCTGGAAACATGGCTGCC
T3081	10304-10305	HYPH	denotes	-
T3082	10305-10306	CD	denotes	3
T3083	10306-10307	SYM	denotes	′
T3084	10307-10309	,	denotes	,
T3085	10309-10317	NN	denotes	Slc39a10
T3086	10317-10319	:	denotes	:
T3087	10319-10320	CD	denotes	5
T3089	10320-10321	SYM	denotes	′
T3090	10321-10322	HYPH	denotes	-
T3088	10322-10343	NN	denotes	GCTGTGGCTGGTAGTAAAAGC
T3091	10343-10344	HYPH	denotes	-
T3092	10344-10345	CD	denotes	3
T3093	10345-10346	SYM	denotes	′
T3094	10346-10347	:	denotes	;
T3095	10348-10349	CD	denotes	5
T3097	10349-10350	SYM	denotes	′
T3098	10350-10351	HYPH	denotes	-
T3096	10351-10372	NN	denotes	GTGGCATGGGATGTAAACAGC
T3099	10372-10373	HYPH	denotes	-
T3100	10373-10374	CD	denotes	3
T3101	10374-10375	SYM	denotes	′
T3102	10375-10376	.	denotes	.
T3137	10378-10380	NN	denotes	S1
T3139	10381-10389	NN	denotes	nuclease
T3138	10390-10397	NN	denotes	mapping
T3140	10398-10400	IN	denotes	of
T3141	10401-10412	NNS	denotes	transcripts
T3142	10413-10414	-LRB-	denotes	(
T3144	10414-10416	NN	denotes	S1
T3143	10417-10425	NN	denotes	analysis
T3145	10425-10426	-RRB-	denotes	)
T3146	10426-10563	sentence	denotes	S1 analysis was performed as previously described (29), using 100 µg DNase I-digested total RNA (RNA isolation see microarray analysis).
T3147	10427-10429	NN	denotes	S1
T3148	10430-10438	NN	denotes	analysis
T3150	10439-10442	VBD	denotes	was
T3149	10443-10452	VBN	denotes	performed
T3151	10453-10455	IN	denotes	as
T3153	10456-10466	RB	denotes	previously
T3152	10467-10476	VBN	denotes	described
T3154	10477-10478	-LRB-	denotes	(
T3155	10478-10480	CD	denotes	29
T3156	10480-10481	-RRB-	denotes	)
T3157	10481-10483	,	denotes	,
T3158	10483-10488	VBG	denotes	using
T3159	10489-10492	CD	denotes	100
T3160	10493-10495	NN	denotes	µg
T3162	10496-10501	NN	denotes	DNase
T3164	10502-10503	CD	denotes	I
T3165	10503-10504	HYPH	denotes	-
T3163	10504-10512	VBN	denotes	digested
T3166	10513-10518	JJ	denotes	total
T3161	10519-10522	NN	denotes	RNA
T3167	10523-10524	-LRB-	denotes	(
T3169	10524-10527	NN	denotes	RNA
T3170	10528-10537	NN	denotes	isolation
T3168	10538-10541	VBP	denotes	see
T3171	10542-10552	NN	denotes	microarray
T3172	10553-10561	NN	denotes	analysis
T3173	10561-10562	-RRB-	denotes	)
T3174	10562-10563	.	denotes	.
T3175	10563-10630	sentence	denotes	The gels were developed using PhosphorImager (Molecular Dynamics).
T3176	10564-10567	DT	denotes	The
T3177	10568-10572	NNS	denotes	gels
T3179	10573-10577	VBD	denotes	were
T3178	10578-10587	VBN	denotes	developed
T3180	10588-10593	VBG	denotes	using
T3181	10594-10608	NNP	denotes	PhosphorImager
T3182	10609-10610	-LRB-	denotes	(
T3184	10610-10619	NNP	denotes	Molecular
T3183	10620-10628	NNP	denotes	Dynamics
T3185	10628-10629	-RRB-	denotes	)
T3186	10629-10630	.	denotes	.
T3187	10630-10897	sentence	denotes	S1 analysis was done with the following 32P-labeled oligonucleotides: Hprt S1: 5′-TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG-3′, Sepw1 S1: 5′-TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC-3′.
T3188	10631-10633	NN	denotes	S1
T3189	10634-10642	NN	denotes	analysis
T3191	10643-10646	VBD	denotes	was
T3190	10647-10651	VBN	denotes	done
T3192	10652-10656	IN	denotes	with
T3193	10657-10660	DT	denotes	the
T3195	10661-10670	VBG	denotes	following
T3196	10671-10674	NN	denotes	32P
T3198	10674-10675	HYPH	denotes	-
T3197	10675-10682	VBN	denotes	labeled
T3194	10683-10699	NNS	denotes	oligonucleotides
T3199	10699-10701	:	denotes	:
T3200	10701-10705	NN	denotes	Hprt
T3201	10706-10708	NN	denotes	S1
T3202	10708-10710	:	denotes	:
T3203	10710-10711	CD	denotes	5
T3205	10711-10712	SYM	denotes	′
T3206	10712-10713	HYPH	denotes	-
T3204	10713-10785	NN	denotes	TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG
T3207	10785-10786	HYPH	denotes	-
T3208	10786-10787	CD	denotes	3
T3209	10787-10788	SYM	denotes	′
T3210	10788-10790	,	denotes	,
T3211	10790-10795	NN	denotes	Sepw1
T3212	10796-10798	NN	denotes	S1
T3213	10798-10800	:	denotes	:
T3214	10800-10801	CD	denotes	5
T3216	10801-10802	SYM	denotes	′
T3217	10802-10803	HYPH	denotes	-
T3215	10803-10893	NN	denotes	TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC
T3218	10893-10894	HYPH	denotes	-
T3219	10894-10895	CD	denotes	3
T3220	10895-10896	SYM	denotes	′
T3221	10896-10897	.	denotes	.
T3351	10899-10914	JJ	denotes	Electrophoretic
T3353	10915-10923	NN	denotes	mobility
T3352	10924-10929	NN	denotes	shift
T3354	10930-10935	NN	denotes	assay
T3355	10936-10937	-LRB-	denotes	(
T3356	10937-10941	NN	denotes	EMSA
T3357	10941-10942	-RRB-	denotes	)
T3358	10942-11146	sentence	denotes	Protein was extracted from liver tissue with T-PER™ Tissue Protein Extraction Reagent (Pierce) according to the manufacturer's instructions, using a 1:10 ratio of mouse tissue (mg) to T-PER reagent (µl).
T3359	10943-10950	NN	denotes	Protein
T3361	10951-10954	VBD	denotes	was
T3360	10955-10964	VBN	denotes	extracted
T3362	10965-10969	IN	denotes	from
T3363	10970-10975	NN	denotes	liver
T3364	10976-10982	NN	denotes	tissue
T3365	10983-10987	IN	denotes	with
T3366	10988-10989	NN	denotes	T
T3368	10989-10990	HYPH	denotes	-
T3367	10990-10993	NN	denotes	PER
T3369	10993-10994	SYM	denotes	™
T3370	10995-11001	NN	denotes	Tissue
T3371	11002-11009	NN	denotes	Protein
T3372	11010-11020	NN	denotes	Extraction
T3373	11021-11028	NN	denotes	Reagent
T3374	11029-11030	-LRB-	denotes	(
T3375	11030-11036	NNP	denotes	Pierce
T3376	11036-11037	-RRB-	denotes	)
T3377	11038-11047	VBG	denotes	according
T3378	11048-11050	IN	denotes	to
T3379	11051-11054	DT	denotes	the
T3380	11055-11067	NN	denotes	manufacturer
T3382	11067-11069	POS	denotes	's
T3381	11070-11082	NNS	denotes	instructions
T3383	11082-11084	,	denotes	,
T3384	11084-11089	VBG	denotes	using
T3385	11090-11091	DT	denotes	a
T3387	11092-11093	CD	denotes	1
T3388	11093-11094	SYM	denotes	:
T3389	11094-11096	CD	denotes	10
T3386	11097-11102	NN	denotes	ratio
T3390	11103-11105	IN	denotes	of
T3391	11106-11111	NN	denotes	mouse
T3392	11112-11118	NN	denotes	tissue
T3393	11119-11120	-LRB-	denotes	(
T3394	11120-11122	NN	denotes	mg
T3395	11122-11123	-RRB-	denotes	)
T3396	11124-11126	IN	denotes	to
T3397	11127-11128	NN	denotes	T
T3399	11128-11129	HYPH	denotes	-
T3398	11129-11132	NN	denotes	PER
T3400	11133-11140	NN	denotes	reagent
T3401	11141-11142	-LRB-	denotes	(
T3402	11142-11144	NN	denotes	µl
T3403	11144-11145	-RRB-	denotes	)
T3404	11145-11146	.	denotes	.
T3405	11146-11207	sentence	denotes	EMSA was essentially performed as previously described (10).
T3406	11147-11151	NN	denotes	EMSA
T3408	11152-11155	VBD	denotes	was
T3409	11156-11167	RB	denotes	essentially
T3407	11168-11177	VBN	denotes	performed
T3410	11178-11180	IN	denotes	as
T3412	11181-11191	RB	denotes	previously
T3411	11192-11201	VBN	denotes	described
T3413	11202-11203	-LRB-	denotes	(
T3414	11203-11205	CD	denotes	10
T3415	11205-11206	-RRB-	denotes	)
T3416	11206-11207	.	denotes	.
T3417	11207-11360	sentence	denotes	All binding reactions were carried out by incubating 2–5 fmol 32P-end-labeled double-stranded oligonucleotides with 100 to 130 µg liver protein extract.
T3418	11208-11211	DT	denotes	All
T3420	11212-11219	NN	denotes	binding
T3419	11220-11229	NNS	denotes	reactions
T3422	11230-11234	VBD	denotes	were
T3421	11235-11242	VBN	denotes	carried
T3423	11243-11246	RP	denotes	out
T3424	11247-11249	IN	denotes	by
T3425	11250-11260	VBG	denotes	incubating
T3426	11261-11262	CD	denotes	2
T3428	11262-11263	SYM	denotes	–
T3427	11263-11264	CD	denotes	5
T3429	11265-11269	NN	denotes	fmol
T3431	11270-11273	NN	denotes	32P
T3433	11273-11274	HYPH	denotes	-
T3434	11274-11277	NN	denotes	end
T3435	11277-11278	HYPH	denotes	-
T3432	11278-11285	VBN	denotes	labeled
T3436	11286-11292	JJ	denotes	double
T3438	11292-11293	HYPH	denotes	-
T3437	11293-11301	VBN	denotes	stranded
T3430	11302-11318	NNS	denotes	oligonucleotides
T3439	11319-11323	IN	denotes	with
T3440	11324-11327	CD	denotes	100
T3442	11328-11330	IN	denotes	to
T3441	11331-11334	CD	denotes	130
T3443	11335-11337	NN	denotes	µg
T3445	11338-11343	NN	denotes	liver
T3446	11344-11351	NN	denotes	protein
T3444	11352-11359	NN	denotes	extract
T3447	11359-11360	.	denotes	.
T3448	11360-11502	sentence	denotes	For competition experiments, 5 pmol of unlabeled competitor oligonucleotide was added to the binding reaction before addition of the extract.
T3449	11361-11364	IN	denotes	For
T3451	11365-11376	NN	denotes	competition
T3452	11377-11388	NNS	denotes	experiments
T3453	11388-11390	,	denotes	,
T3454	11390-11391	CD	denotes	5
T3455	11392-11396	NN	denotes	pmol
T3456	11397-11399	IN	denotes	of
T3457	11400-11409	JJ	denotes	unlabeled
T3459	11410-11420	NN	denotes	competitor
T3458	11421-11436	NN	denotes	oligonucleotide
T3460	11437-11440	VBD	denotes	was
T3450	11441-11446	VBN	denotes	added
T3461	11447-11449	IN	denotes	to
T3462	11450-11453	DT	denotes	the
T3464	11454-11461	NN	denotes	binding
T3463	11462-11470	NN	denotes	reaction
T3465	11471-11477	IN	denotes	before
T3466	11478-11486	NN	denotes	addition
T3467	11487-11489	IN	denotes	of
T3468	11490-11493	DT	denotes	the
T3469	11494-11501	NN	denotes	extract
T3470	11501-11502	.	denotes	.
T3471	11502-11574	sentence	denotes	All EMSA gels were developed using PhosphorImager (Molecular Dynamics).
T3472	11503-11506	DT	denotes	All
T3474	11507-11511	NN	denotes	EMSA
T3473	11512-11516	NNS	denotes	gels
T3476	11517-11521	VBD	denotes	were
T3475	11522-11531	VBN	denotes	developed
T3477	11532-11537	VBG	denotes	using
T3478	11538-11552	NNP	denotes	PhosphorImager
T3479	11553-11554	-LRB-	denotes	(
T3481	11554-11563	NNP	denotes	Molecular
T3480	11564-11572	NNP	denotes	Dynamics
T3482	11572-11573	-RRB-	denotes	)
T3483	11573-11574	.	denotes	.
T3484	11574-13036	sentence	denotes	The following oligonucleotides were annealed and used for the reactions: Csrp1 MRE1: 5′-GGAAACAAAACGGCGCGCACTCCGGCGC-3′; 5′-GGCTGCGC CGGAGTGCGCGCCGTTTTGT-3′, Csrp1 MRE2: 5′-TGTTGTGGTGCAGTGTGCAAAGCCTAC-3′; 5′-ACCAGTAGGCTTTGCACACTGCACCAC-3′, Csrp1 MRE3: 5′-GAGATCGCCATAGGGTGCAAAGAGAAG-3′; 5′-GTGACTTCTCTTTGCACCCTATGGCGA-3′, Csrp1 MRE4: 5′-TGTCTTATTCTGGAGTGCAAGTTAGTC-3′; 5′-AGGGGACTAACTTGCACTCCAGAATAA-3′, Gal4: 5′-TCCGGAGGACTGTCCTCCGG-3′; 5′-GCCGGAGGACAGTCCTCCGG-3′, MREd [MRE derived from mouse Mt1 promoter (10)]: 5′-CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG-3′; 5′-TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT-3′, MRE-s [synthetic MRE consensus sequence (10)]: 5′-CGAGGGAGCTCTGCACACGGCCCGAAAAGTG-3′; 5′-TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT-3′, Ndrg1 MRE1: 5′-CAGCCCAGGCAGGGTGCAGCACGAG-3′; 5′-CCGCCTCGTGCTGCACCCTGCCTGG-3′, Ndrg1 MRE2: 5′-CACACGTTCGCTGCACACGCCGCGG-3′; 5′-GGGACCGCGGCGTGTGCAGCGAACG-3′, Ndrg1 MRE3,4: 5′-GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC-3′; 5′-TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC-3′, Sepw1 MRE1: 5′-GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC-3′; 5′-CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC-3′, Sepw1 MRE2: 5′-ATGGTTTTGGGGGTGCGCAGGGGGTCTG-3′; 5′-CGACAGACCCCCTGCGCACCCCCAAAAC-3′, Slc39a10 MRE1: 5′-GAATACACGACTGGGTGCAGCCGGGGTTTGG-3′; 5′-GGTACCAAACCCCGGCTGCACCCAGTCGTGTA-3′, Slc39a10 MRE2: 5′-GCGGAGAGGAGATGCACACGGCACTCG-3′; 5′-CACTCGAGTGCCGTGTGCATCTCCTCT-3′, Specificity protein 1 (Sp1) binding sequence (10): 5′-CGAGGCCCCGCCCAG-3′; 5′-TCGACTGGGCGGGGCCTCGAGct-3′.
T3485	11575-11578	DT	denotes	The
T3487	11579-11588	VBG	denotes	following
T3486	11589-11605	NNS	denotes	oligonucleotides
T3489	11606-11610	VBD	denotes	were
T3488	11611-11619	VBN	denotes	annealed
T3490	11620-11623	CC	denotes	and
T3491	11624-11628	VBN	denotes	used
T3492	11629-11632	IN	denotes	for
T3493	11633-11636	DT	denotes	the
T3494	11637-11646	NNS	denotes	reactions
T3495	11646-11648	:	denotes	:
T3496	11648-11653	NN	denotes	Csrp1
T3497	11654-11658	NN	denotes	MRE1
T3498	11658-11660	:	denotes	:
T3499	11660-11661	CD	denotes	5
T3501	11661-11662	SYM	denotes	′
T3502	11662-11663	HYPH	denotes	-
T3500	11663-11691	NN	denotes	GGAAACAAAACGGCGCGCACTCCGGCGC
T3503	11691-11692	HYPH	denotes	-
T3504	11692-11693	CD	denotes	3
T3505	11693-11694	SYM	denotes	′
T3506	11694-11695	:	denotes	;
T3507	11696-11697	CD	denotes	5
T3509	11697-11698	SYM	denotes	′
T3510	11698-11699	HYPH	denotes	-
T3511	11699-11707	NN	denotes	GGCTGCGC
T3508	11708-11728	NN	denotes	CGGAGTGCGCGCCGTTTTGT
T3512	11728-11729	HYPH	denotes	-
T3513	11729-11730	CD	denotes	3
T3514	11730-11731	SYM	denotes	′
T3515	11731-11733	,	denotes	,
T3516	11733-11738	NN	denotes	Csrp1
T3517	11739-11743	NN	denotes	MRE2
T3518	11743-11745	:	denotes	:
T3519	11745-11746	CD	denotes	5
T3521	11746-11747	SYM	denotes	′
T3522	11747-11748	HYPH	denotes	-
T3520	11748-11775	NN	denotes	TGTTGTGGTGCAGTGTGCAAAGCCTAC
T3523	11775-11776	HYPH	denotes	-
T3524	11776-11777	CD	denotes	3
T3525	11777-11778	SYM	denotes	′
T3526	11778-11779	:	denotes	;
T3527	11780-11781	CD	denotes	5
T3529	11781-11782	SYM	denotes	′
T3530	11782-11783	HYPH	denotes	-
T3528	11783-11810	NN	denotes	ACCAGTAGGCTTTGCACACTGCACCAC
T3531	11810-11811	HYPH	denotes	-
T3532	11811-11812	CD	denotes	3
T3533	11812-11813	SYM	denotes	′
T3534	11813-11815	,	denotes	,
T3535	11815-11820	NN	denotes	Csrp1
T3536	11821-11825	NN	denotes	MRE3
T3537	11825-11827	:	denotes	:
T3538	11827-11828	CD	denotes	5
T3540	11828-11829	SYM	denotes	′
T3541	11829-11830	HYPH	denotes	-
T3539	11830-11857	NN	denotes	GAGATCGCCATAGGGTGCAAAGAGAAG
T3542	11857-11858	HYPH	denotes	-
T3543	11858-11859	CD	denotes	3
T3544	11859-11860	SYM	denotes	′
T3545	11860-11861	:	denotes	;
T3546	11862-11863	CD	denotes	5
T3548	11863-11864	SYM	denotes	′
T3549	11864-11865	HYPH	denotes	-
T3547	11865-11892	NN	denotes	GTGACTTCTCTTTGCACCCTATGGCGA
T3550	11892-11893	HYPH	denotes	-
T3551	11893-11894	CD	denotes	3
T3552	11894-11895	SYM	denotes	′
T3553	11895-11897	,	denotes	,
T3554	11897-11902	NN	denotes	Csrp1
T3555	11903-11907	NN	denotes	MRE4
T3556	11907-11909	:	denotes	:
T3557	11909-11910	CD	denotes	5
T3559	11910-11911	SYM	denotes	′
T3560	11911-11912	HYPH	denotes	-
T3558	11912-11939	NN	denotes	TGTCTTATTCTGGAGTGCAAGTTAGTC
T3561	11939-11940	HYPH	denotes	-
T3562	11940-11941	CD	denotes	3
T3563	11941-11942	SYM	denotes	′
T3564	11942-11943	:	denotes	;
T3565	11944-11945	CD	denotes	5
T3567	11945-11946	SYM	denotes	′
T3568	11946-11947	HYPH	denotes	-
T3566	11947-11974	NN	denotes	AGGGGACTAACTTGCACTCCAGAATAA
T3569	11974-11975	HYPH	denotes	-
T3570	11975-11976	CD	denotes	3
T3571	11976-11977	SYM	denotes	′
T3572	11977-11979	,	denotes	,
T3573	11979-11983	NN	denotes	Gal4
T3574	11983-11985	:	denotes	:
T3575	11985-11986	CD	denotes	5
T3577	11986-11987	SYM	denotes	′
T3578	11987-11988	HYPH	denotes	-
T3576	11988-12008	NN	denotes	TCCGGAGGACTGTCCTCCGG
T3579	12008-12009	HYPH	denotes	-
T3580	12009-12010	CD	denotes	3
T3581	12010-12011	SYM	denotes	′
T3582	12011-12012	:	denotes	;
T3583	12013-12014	CD	denotes	5
T3585	12014-12015	SYM	denotes	′
T3586	12015-12016	HYPH	denotes	-
T3584	12016-12036	NN	denotes	GCCGGAGGACAGTCCTCCGG
T3587	12036-12037	HYPH	denotes	-
T3588	12037-12038	CD	denotes	3
T3589	12038-12039	SYM	denotes	′
T3590	12039-12041	,	denotes	,
T3591	12041-12045	NN	denotes	MREd
T3592	12046-12047	-LRB-	denotes	[
T3593	12047-12050	NN	denotes	MRE
T3594	12051-12058	VBN	denotes	derived
T3595	12059-12063	IN	denotes	from
T3596	12064-12069	NN	denotes	mouse
T3598	12070-12073	NN	denotes	Mt1
T3597	12074-12082	NN	denotes	promoter
T3599	12083-12084	-LRB-	denotes	(
T3600	12084-12086	CD	denotes	10
T3601	12086-12087	-RRB-	denotes	)
T3602	12087-12088	-RRB-	denotes	]
T3603	12088-12090	:	denotes	:
T3604	12090-12091	CD	denotes	5
T3606	12091-12092	SYM	denotes	′
T3607	12092-12093	HYPH	denotes	-
T3605	12093-12124	NN	denotes	CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
T3608	12124-12125	HYPH	denotes	-
T3609	12125-12126	CD	denotes	3
T3610	12126-12127	SYM	denotes	′
T3611	12127-12128	:	denotes	;
T3612	12129-12130	CD	denotes	5
T3614	12130-12131	SYM	denotes	′
T3615	12131-12132	HYPH	denotes	-
T3613	12132-12171	NN	denotes	TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT
T3616	12171-12172	HYPH	denotes	-
T3617	12172-12173	CD	denotes	3
T3618	12173-12174	SYM	denotes	′
T3619	12174-12176	,	denotes	,
T3620	12176-12179	NN	denotes	MRE
T3622	12179-12180	HYPH	denotes	-
T3621	12180-12181	NN	denotes	s
T3623	12182-12183	-LRB-	denotes	[
T3624	12183-12192	JJ	denotes	synthetic
T3626	12193-12196	NN	denotes	MRE
T3627	12197-12206	NN	denotes	consensus
T3625	12207-12215	NN	denotes	sequence
T3628	12216-12217	-LRB-	denotes	(
T3629	12217-12219	CD	denotes	10
T3630	12219-12220	-RRB-	denotes	)
T3631	12220-12221	-RRB-	denotes	]
T3632	12221-12223	:	denotes	:
T3633	12223-12224	CD	denotes	5
T3635	12224-12225	SYM	denotes	′
T3636	12225-12226	HYPH	denotes	-
T3634	12226-12257	NN	denotes	CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
T3637	12257-12258	HYPH	denotes	-
T3638	12258-12259	CD	denotes	3
T3639	12259-12260	SYM	denotes	′
T3640	12260-12261	:	denotes	;
T3641	12262-12263	CD	denotes	5
T3643	12263-12264	SYM	denotes	′
T3644	12264-12265	HYPH	denotes	-
T3642	12265-12304	NN	denotes	TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT
T3645	12304-12305	HYPH	denotes	-
T3646	12305-12306	CD	denotes	3
T3647	12306-12307	SYM	denotes	′
T3648	12307-12309	,	denotes	,
T3649	12309-12314	NN	denotes	Ndrg1
T3650	12315-12319	NN	denotes	MRE1
T3651	12319-12321	:	denotes	:
T3652	12321-12322	CD	denotes	5
T3654	12322-12323	SYM	denotes	′
T3655	12323-12324	HYPH	denotes	-
T3653	12324-12349	NN	denotes	CAGCCCAGGCAGGGTGCAGCACGAG
T3656	12349-12350	HYPH	denotes	-
T3657	12350-12351	CD	denotes	3
T3658	12351-12352	SYM	denotes	′
T3659	12352-12353	:	denotes	;
T3660	12354-12355	CD	denotes	5
T3662	12355-12356	SYM	denotes	′
T3663	12356-12357	HYPH	denotes	-
T3661	12357-12382	NN	denotes	CCGCCTCGTGCTGCACCCTGCCTGG
T3664	12382-12383	HYPH	denotes	-
T3665	12383-12384	CD	denotes	3
T3666	12384-12385	SYM	denotes	′
T3667	12385-12387	,	denotes	,
T3668	12387-12392	NN	denotes	Ndrg1
T3669	12393-12397	NN	denotes	MRE2
T3670	12397-12399	:	denotes	:
T3671	12399-12400	CD	denotes	5
T3673	12400-12401	SYM	denotes	′
T3674	12401-12402	HYPH	denotes	-
T3672	12402-12427	NN	denotes	CACACGTTCGCTGCACACGCCGCGG
T3675	12427-12428	HYPH	denotes	-
T3676	12428-12429	CD	denotes	3
T3677	12429-12430	SYM	denotes	′
T3678	12430-12431	:	denotes	;
T3679	12432-12433	CD	denotes	5
T3681	12433-12434	SYM	denotes	′
T3682	12434-12435	HYPH	denotes	-
T3680	12435-12460	NN	denotes	GGGACCGCGGCGTGTGCAGCGAACG
T3683	12460-12461	HYPH	denotes	-
T3684	12461-12462	CD	denotes	3
T3685	12462-12463	SYM	denotes	′
T3686	12463-12465	,	denotes	,
T3687	12465-12470	NN	denotes	Ndrg1
T3688	12471-12475	NN	denotes	MRE3
T3689	12475-12476	,	denotes	,
T3690	12476-12477	CD	denotes	4
T3691	12477-12479	:	denotes	:
T3692	12479-12480	CD	denotes	5
T3694	12480-12481	SYM	denotes	′
T3695	12481-12482	HYPH	denotes	-
T3693	12482-12521	NN	denotes	GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
T3696	12521-12522	HYPH	denotes	-
T3697	12522-12523	CD	denotes	3
T3698	12523-12524	SYM	denotes	′
T3699	12524-12525	:	denotes	;
T3700	12526-12527	CD	denotes	5
T3702	12527-12528	SYM	denotes	′
T3703	12528-12529	HYPH	denotes	-
T3701	12529-12569	NN	denotes	TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC
T3704	12569-12570	HYPH	denotes	-
T3705	12570-12571	CD	denotes	3
T3706	12571-12572	SYM	denotes	′
T3707	12572-12574	,	denotes	,
T3708	12574-12579	NN	denotes	Sepw1
T3709	12580-12584	NN	denotes	MRE1
T3710	12584-12586	:	denotes	:
T3711	12586-12587	CD	denotes	5
T3713	12587-12588	SYM	denotes	′
T3714	12588-12589	HYPH	denotes	-
T3712	12589-12622	NN	denotes	GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
T3715	12622-12623	HYPH	denotes	-
T3716	12623-12624	CD	denotes	3
T3717	12624-12625	SYM	denotes	′
T3718	12625-12626	:	denotes	;
T3719	12627-12628	CD	denotes	5
T3721	12628-12629	SYM	denotes	′
T3722	12629-12630	HYPH	denotes	-
T3720	12630-12664	NN	denotes	CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC
T3723	12664-12665	HYPH	denotes	-
T3724	12665-12666	CD	denotes	3
T3725	12666-12667	SYM	denotes	′
T3726	12667-12669	,	denotes	,
T3727	12669-12674	NN	denotes	Sepw1
T3728	12675-12679	NN	denotes	MRE2
T3729	12679-12681	:	denotes	:
T3730	12681-12682	CD	denotes	5
T3732	12682-12683	SYM	denotes	′
T3733	12683-12684	HYPH	denotes	-
T3731	12684-12712	NN	denotes	ATGGTTTTGGGGGTGCGCAGGGGGTCTG
T3734	12712-12713	HYPH	denotes	-
T3735	12713-12714	CD	denotes	3
T3736	12714-12715	SYM	denotes	′
T3737	12715-12716	:	denotes	;
T3738	12717-12718	CD	denotes	5
T3740	12718-12719	SYM	denotes	′
T3741	12719-12720	HYPH	denotes	-
T3739	12720-12748	NN	denotes	CGACAGACCCCCTGCGCACCCCCAAAAC
T3742	12748-12749	HYPH	denotes	-
T3743	12749-12750	CD	denotes	3
T3744	12750-12751	SYM	denotes	′
T3745	12751-12753	,	denotes	,
T3746	12753-12761	NN	denotes	Slc39a10
T3747	12762-12766	NN	denotes	MRE1
T3748	12766-12768	:	denotes	:
T3749	12768-12769	CD	denotes	5
T3751	12769-12770	SYM	denotes	′
T3752	12770-12771	HYPH	denotes	-
T3750	12771-12802	NN	denotes	GAATACACGACTGGGTGCAGCCGGGGTTTGG
T3753	12802-12803	HYPH	denotes	-
T3754	12803-12804	CD	denotes	3
T3755	12804-12805	SYM	denotes	′
T3756	12805-12806	:	denotes	;
T3757	12807-12808	CD	denotes	5
T3759	12808-12809	SYM	denotes	′
T3760	12809-12810	HYPH	denotes	-
T3758	12810-12842	NN	denotes	GGTACCAAACCCCGGCTGCACCCAGTCGTGTA
T3761	12842-12843	HYPH	denotes	-
T3762	12843-12844	CD	denotes	3
T3763	12844-12845	SYM	denotes	′
T3764	12845-12847	,	denotes	,
T3765	12847-12855	NN	denotes	Slc39a10
T3766	12856-12860	NN	denotes	MRE2
T3767	12860-12862	:	denotes	:
T3768	12862-12863	CD	denotes	5
T3770	12863-12864	SYM	denotes	′
T3771	12864-12865	HYPH	denotes	-
T3769	12865-12892	NN	denotes	GCGGAGAGGAGATGCACACGGCACTCG
T3772	12892-12893	HYPH	denotes	-
T3773	12893-12894	CD	denotes	3
T3774	12894-12895	SYM	denotes	′
T3775	12895-12896	:	denotes	;
T3776	12897-12898	CD	denotes	5
T3778	12898-12899	SYM	denotes	′
T3779	12899-12900	HYPH	denotes	-
T3777	12900-12927	NN	denotes	CACTCGAGTGCCGTGTGCATCTCCTCT
T3780	12927-12928	HYPH	denotes	-
T3781	12928-12929	CD	denotes	3
T3782	12929-12930	SYM	denotes	′
T3783	12930-12932	,	denotes	,
T3784	12932-12943	NN	denotes	Specificity
T3785	12944-12951	NN	denotes	protein
T3787	12952-12953	CD	denotes	1
T3788	12954-12955	-LRB-	denotes	(
T3789	12955-12958	NN	denotes	Sp1
T3790	12958-12959	-RRB-	denotes	)
T3791	12960-12967	NN	denotes	binding
T3786	12968-12976	NN	denotes	sequence
T3792	12977-12978	-LRB-	denotes	(
T3793	12978-12980	CD	denotes	10
T3794	12980-12981	-RRB-	denotes	)
T3795	12981-12983	:	denotes	:
T3796	12983-12984	CD	denotes	5
T3798	12984-12985	SYM	denotes	′
T3799	12985-12986	HYPH	denotes	-
T3797	12986-13001	NN	denotes	CGAGGCCCCGCCCAG
T3800	13001-13002	HYPH	denotes	-
T3801	13002-13003	CD	denotes	3
T3802	13003-13004	SYM	denotes	′
T3803	13004-13005	:	denotes	;
T3804	13006-13007	CD	denotes	5
T3806	13007-13008	SYM	denotes	′
T3807	13008-13009	HYPH	denotes	-
T3805	13009-13032	NN	denotes	TCGACTGGGCGGGGCCTCGAGct
T3808	13032-13033	HYPH	denotes	-
T3809	13033-13034	CD	denotes	3
T3810	13034-13035	SYM	denotes	′
T3811	13035-13036	.	denotes	.
T3875	13038-13042	NN	denotes	Cell
T3876	13043-13050	NN	denotes	culture
T3877	13050-13281	sentence	denotes	Primary embryonic fibroblasts were isolated from a 12.5 day old Mtf1loxP mouse embryo and grown in DMEM supplemented with 10% fetal bovine serum (ICN), 100 U/ml penicillin–streptomycin (Gibco BRL) and 2 mM l-glutamine (Gibco BRL).
T3878	13051-13058	JJ	denotes	Primary
T3880	13059-13068	JJ	denotes	embryonic
T3879	13069-13080	NNS	denotes	fibroblasts
T3882	13081-13085	VBD	denotes	were
T3881	13086-13094	VBN	denotes	isolated
T3883	13095-13099	IN	denotes	from
T3884	13100-13101	DT	denotes	a
T3886	13102-13106	CD	denotes	12.5
T3885	13107-13110	NN	denotes	day
T3887	13111-13114	JJ	denotes	old
T3873	13115-13123	NN	denotes	Mtf1loxP
T3889	13124-13129	NN	denotes	mouse
T3888	13130-13136	NN	denotes	embryo
T3890	13137-13140	CC	denotes	and
T3891	13141-13146	VBN	denotes	grown
T3892	13147-13149	IN	denotes	in
T3893	13150-13154	NN	denotes	DMEM
T3894	13155-13167	VBN	denotes	supplemented
T3895	13168-13172	IN	denotes	with
T3896	13173-13175	CD	denotes	10
T3897	13175-13176	NN	denotes	%
T3899	13177-13182	JJ	denotes	fetal
T3900	13183-13189	JJ	denotes	bovine
T3898	13190-13195	NN	denotes	serum
T3901	13196-13197	-LRB-	denotes	(
T3902	13197-13200	NN	denotes	ICN
T3903	13200-13201	-RRB-	denotes	)
T3904	13201-13203	,	denotes	,
T3905	13203-13206	CD	denotes	100
T3906	13207-13208	NN	denotes	U
T3907	13208-13209	SYM	denotes	/
T3908	13209-13211	NN	denotes	ml
T3909	13212-13222	NN	denotes	penicillin
T3910	13222-13223	HYPH	denotes	–
T3874	13223-13235	NN	denotes	streptomycin
T3911	13236-13237	-LRB-	denotes	(
T3913	13237-13242	NNP	denotes	Gibco
T3912	13243-13246	NNP	denotes	BRL
T3914	13246-13247	-RRB-	denotes	)
T3915	13248-13251	CC	denotes	and
T3916	13252-13253	CD	denotes	2
T3917	13254-13256	NN	denotes	mM
T3919	13257-13258	NN	denotes	l
T3920	13258-13259	HYPH	denotes	-
T3918	13259-13268	NN	denotes	glutamine
T3921	13269-13270	-LRB-	denotes	(
T3923	13270-13275	NNP	denotes	Gibco
T3922	13276-13279	NNP	denotes	BRL
T3924	13279-13280	-RRB-	denotes	)
T3925	13280-13281	.	denotes	.
T3926	13281-13551	sentence	denotes	100 mm plates with primary cells were transfected with 10 µg of an expression plasmid coding for simian virus 40 (SV40) large T antigen driven by the cytomegalovirus (CMV) promoter, using lipofectamine™ reagent (Invitrogen) according to the manufacturer's instructions.
T3927	13282-13285	CD	denotes	100
T3928	13286-13288	NN	denotes	mm
T3929	13289-13295	NNS	denotes	plates
T3931	13296-13300	IN	denotes	with
T3932	13301-13308	JJ	denotes	primary
T3933	13309-13314	NNS	denotes	cells
T3934	13315-13319	VBD	denotes	were
T3930	13320-13331	VBN	denotes	transfected
T3935	13332-13336	IN	denotes	with
T3936	13337-13339	CD	denotes	10
T3937	13340-13342	NN	denotes	µg
T3938	13343-13345	IN	denotes	of
T3939	13346-13348	DT	denotes	an
T3941	13349-13359	NN	denotes	expression
T3940	13360-13367	NN	denotes	plasmid
T3942	13368-13374	VBG	denotes	coding
T3943	13375-13378	IN	denotes	for
T3944	13379-13385	JJ	denotes	simian
T3945	13386-13391	NN	denotes	virus
T3947	13392-13394	CD	denotes	40
T3948	13395-13396	-LRB-	denotes	(
T3949	13396-13400	NN	denotes	SV40
T3950	13400-13401	-RRB-	denotes	)
T3951	13402-13407	JJ	denotes	large
T3952	13408-13409	NN	denotes	T
T3946	13410-13417	NN	denotes	antigen
T3953	13418-13424	VBN	denotes	driven
T3954	13425-13427	IN	denotes	by
T3955	13428-13431	DT	denotes	the
T3957	13432-13447	NN	denotes	cytomegalovirus
T3958	13448-13449	-LRB-	denotes	(
T3959	13449-13452	NN	denotes	CMV
T3960	13452-13453	-RRB-	denotes	)
T3956	13454-13462	NN	denotes	promoter
T3961	13462-13464	,	denotes	,
T3962	13464-13469	VBG	denotes	using
T3963	13470-13483	NNP	denotes	lipofectamine
T3965	13483-13484	SYM	denotes	™
T3964	13485-13492	NN	denotes	reagent
T3966	13493-13494	-LRB-	denotes	(
T3967	13494-13504	NNP	denotes	Invitrogen
T3968	13504-13505	-RRB-	denotes	)
T3969	13506-13515	VBG	denotes	according
T3970	13516-13518	IN	denotes	to
T3971	13519-13522	DT	denotes	the
T3972	13523-13535	NN	denotes	manufacturer
T3974	13535-13537	POS	denotes	's
T3973	13538-13550	NNS	denotes	instructions
T3975	13550-13551	.	denotes	.
T3976	13551-13668	sentence	denotes	Cell foci were isolated and the immortalized mouse embryonic fibroblast cell line ckoC was derived from one of them.
T3977	13552-13556	NN	denotes	Cell
T3978	13557-13561	NNS	denotes	foci
T3980	13562-13566	VBD	denotes	were
T3979	13567-13575	VBN	denotes	isolated
T3981	13576-13579	CC	denotes	and
T3982	13580-13583	DT	denotes	the
T3984	13584-13596	VBN	denotes	immortalized
T3985	13597-13602	NN	denotes	mouse
T3987	13603-13612	JJ	denotes	embryonic
T3986	13613-13623	NN	denotes	fibroblast
T3988	13624-13628	NN	denotes	cell
T3983	13629-13633	NN	denotes	line
T3990	13634-13638	NN	denotes	ckoC
T3991	13639-13642	VBD	denotes	was
T3989	13643-13650	VBN	denotes	derived
T3992	13651-13655	IN	denotes	from
T3993	13656-13659	CD	denotes	one
T3994	13660-13662	IN	denotes	of
T3995	13663-13667	PRP	denotes	them
T3996	13667-13668	.	denotes	.
T3997	13668-13778	sentence	denotes	The Mtf1loxP genotype of this line as well as the genomic integration of the T antigen were confirmed by PCR.
T3998	13669-13672	DT	denotes	The
T4000	13673-13681	NN	denotes	Mtf1loxP
T3999	13682-13690	NN	denotes	genotype
T4002	13691-13693	IN	denotes	of
T4003	13694-13698	DT	denotes	this
T4004	13699-13703	NN	denotes	line
T4005	13704-13706	RB	denotes	as
T4007	13707-13711	RB	denotes	well
T4006	13712-13714	IN	denotes	as
T4008	13715-13718	DT	denotes	the
T4010	13719-13726	JJ	denotes	genomic
T4009	13727-13738	NN	denotes	integration
T4011	13739-13741	IN	denotes	of
T4012	13742-13745	DT	denotes	the
T4014	13746-13747	NN	denotes	T
T4013	13748-13755	NN	denotes	antigen
T4015	13756-13760	VBD	denotes	were
T4001	13761-13770	VBN	denotes	confirmed
T4016	13771-13773	IN	denotes	by
T4017	13774-13777	NN	denotes	PCR
T4018	13777-13778	.	denotes	.
T4019	13778-14063	sentence	denotes	100 mm plates with these cells were further transfected by the calcium phosphate method (30) with 19.6 µg of an expression plasmid for Cre recombinase driven by the CMV promoter and 0.4 µg of an expression plasmid for the neomycin resistance gene under the control of the TK promoter.
T4020	13779-13782	CD	denotes	100
T4021	13783-13785	NN	denotes	mm
T4022	13786-13792	NNS	denotes	plates
T4024	13793-13797	IN	denotes	with
T4025	13798-13803	DT	denotes	these
T4026	13804-13809	NNS	denotes	cells
T4027	13810-13814	VBD	denotes	were
T4028	13815-13822	RB	denotes	further
T4023	13823-13834	VBN	denotes	transfected
T4029	13835-13837	IN	denotes	by
T4030	13838-13841	DT	denotes	the
T4032	13842-13849	NN	denotes	calcium
T4033	13850-13859	NN	denotes	phosphate
T4031	13860-13866	NN	denotes	method
T4034	13867-13868	-LRB-	denotes	(
T4035	13868-13870	CD	denotes	30
T4036	13870-13871	-RRB-	denotes	)
T4037	13872-13876	IN	denotes	with
T4038	13877-13881	CD	denotes	19.6
T4039	13882-13884	NN	denotes	µg
T4040	13885-13887	IN	denotes	of
T4041	13888-13890	DT	denotes	an
T4043	13891-13901	NN	denotes	expression
T4042	13902-13909	NN	denotes	plasmid
T4044	13910-13913	IN	denotes	for
T4045	13914-13917	NN	denotes	Cre
T4046	13918-13929	NN	denotes	recombinase
T4047	13930-13936	VBN	denotes	driven
T4048	13937-13939	IN	denotes	by
T4049	13940-13943	DT	denotes	the
T4051	13944-13947	NN	denotes	CMV
T4050	13948-13956	NN	denotes	promoter
T4052	13957-13960	CC	denotes	and
T4053	13961-13964	CD	denotes	0.4
T4054	13965-13967	NN	denotes	µg
T4055	13968-13970	IN	denotes	of
T4056	13971-13973	DT	denotes	an
T4058	13974-13984	NN	denotes	expression
T4057	13985-13992	NN	denotes	plasmid
T4059	13993-13996	IN	denotes	for
T4060	13997-14000	DT	denotes	the
T4062	14001-14009	NN	denotes	neomycin
T4063	14010-14020	NN	denotes	resistance
T4061	14021-14025	NN	denotes	gene
T4064	14026-14031	IN	denotes	under
T4065	14032-14035	DT	denotes	the
T4066	14036-14043	NN	denotes	control
T4067	14044-14046	IN	denotes	of
T4068	14047-14050	DT	denotes	the
T4070	14051-14053	NN	denotes	TK
T4069	14054-14062	NN	denotes	promoter
T4071	14062-14063	.	denotes	.
T4072	14063-14326	sentence	denotes	Stably transfected cells were selected in the presence of 0.4 µg/µl G418 (Calbiochem), isolated clones of resistant cells were harvested and grown independently, and the expression of Cre recombinase and excision of exons 3 and 4 of Mtf1 were analyzed by RT–PCR.
T4073	14064-14070	RB	denotes	Stably
T4074	14071-14082	VBN	denotes	transfected
T4075	14083-14088	NNS	denotes	cells
T4077	14089-14093	VBD	denotes	were
T4076	14094-14102	VBN	denotes	selected
T4078	14103-14105	IN	denotes	in
T4079	14106-14109	DT	denotes	the
T4080	14110-14118	NN	denotes	presence
T4081	14119-14121	IN	denotes	of
T4082	14122-14125	CD	denotes	0.4
T4083	14126-14128	NN	denotes	µg
T4085	14128-14129	SYM	denotes	/
T4086	14129-14131	NN	denotes	µl
T4084	14132-14136	NN	denotes	G418
T4087	14137-14138	-LRB-	denotes	(
T4088	14138-14148	NNP	denotes	Calbiochem
T4089	14148-14149	-RRB-	denotes	)
T4090	14149-14151	,	denotes	,
T4091	14151-14159	VBN	denotes	isolated
T4092	14160-14166	NNS	denotes	clones
T4094	14167-14169	IN	denotes	of
T4095	14170-14179	JJ	denotes	resistant
T4096	14180-14185	NNS	denotes	cells
T4097	14186-14190	VBD	denotes	were
T4093	14191-14200	VBN	denotes	harvested
T4098	14201-14204	CC	denotes	and
T4099	14205-14210	VBN	denotes	grown
T4100	14211-14224	RB	denotes	independently
T4101	14224-14226	,	denotes	,
T4102	14226-14229	CC	denotes	and
T4103	14230-14233	DT	denotes	the
T4104	14234-14244	NN	denotes	expression
T4106	14245-14247	IN	denotes	of
T4107	14248-14251	NN	denotes	Cre
T4108	14252-14263	NN	denotes	recombinase
T4109	14264-14267	CC	denotes	and
T4110	14268-14276	NN	denotes	excision
T4111	14277-14279	IN	denotes	of
T4112	14280-14285	NNS	denotes	exons
T4113	14286-14287	CD	denotes	3
T4114	14288-14291	CC	denotes	and
T4115	14292-14293	CD	denotes	4
T4116	14294-14296	IN	denotes	of
T4117	14297-14301	NN	denotes	Mtf1
T4118	14302-14306	VBD	denotes	were
T4105	14307-14315	VBN	denotes	analyzed
T4119	14316-14318	IN	denotes	by
T4120	14319-14321	NN	denotes	RT
T4122	14321-14322	HYPH	denotes	–
T4121	14322-14325	NN	denotes	PCR
T4123	14325-14326	.	denotes	.
T4124	14326-14428	sentence	denotes	The cell lines delC19, delC21 and delC23 with a deletion of Mtf1 were chosen for further experiments.
T4125	14327-14330	DT	denotes	The
T4127	14331-14335	NN	denotes	cell
T4126	14336-14341	NNS	denotes	lines
T4129	14342-14348	NN	denotes	delC19
T4130	14348-14350	,	denotes	,
T4131	14350-14356	NN	denotes	delC21
T4132	14357-14360	CC	denotes	and
T4133	14361-14367	NN	denotes	delC23
T4134	14368-14372	IN	denotes	with
T4135	14373-14374	DT	denotes	a
T4136	14375-14383	NN	denotes	deletion
T4137	14384-14386	IN	denotes	of
T4138	14387-14391	NN	denotes	Mtf1
T4139	14392-14396	VBD	denotes	were
T4128	14397-14403	VBN	denotes	chosen
T4140	14404-14407	IN	denotes	for
T4141	14408-14415	JJ	denotes	further
T4142	14416-14427	NNS	denotes	experiments
T4143	14427-14428	.	denotes	.
T4279	14430-14442	NN	denotes	Cytotoxicity
T4280	14443-14448	NN	denotes	assay
T4281	14448-14555	sentence	denotes	Samples of 1 × 104 cells/well were plated in 96-well tissue culture plates and allowed to adhere for 24 h.
T4282	14449-14456	NNS	denotes	Samples
T4284	14457-14459	IN	denotes	of
T4285	14460-14461	CD	denotes	1
T4287	14462-14463	SYM	denotes	×
T4286	14464-14467	CD	denotes	104
T4288	14468-14473	NNS	denotes	cells
T4289	14473-14474	SYM	denotes	/
T4290	14474-14478	NN	denotes	well
T4291	14479-14483	VBD	denotes	were
T4283	14484-14490	VBN	denotes	plated
T4292	14491-14493	IN	denotes	in
T4293	14494-14496	CD	denotes	96
T4295	14496-14497	HYPH	denotes	-
T4294	14497-14501	NN	denotes	well
T4297	14502-14508	NN	denotes	tissue
T4298	14509-14516	NN	denotes	culture
T4296	14517-14523	NNS	denotes	plates
T4299	14524-14527	CC	denotes	and
T4300	14528-14535	VBN	denotes	allowed
T4301	14536-14538	TO	denotes	to
T4302	14539-14545	VB	denotes	adhere
T4303	14546-14549	IN	denotes	for
T4304	14550-14552	CD	denotes	24
T4305	14553-14554	NN	denotes	h
T4306	14554-14555	.	denotes	.
T4307	14555-14736	sentence	denotes	The cells were then pre-incubated for 24 h in medium containing 0, 5, 10, 25 or 50 µM l-buthionine-[S,R]-sulfoximine (BSO) (Sigma), a drug that inhibits glutathione synthesis (31).
T4308	14556-14559	DT	denotes	The
T4309	14560-14565	NNS	denotes	cells
T4311	14566-14570	VBD	denotes	were
T4312	14571-14575	RB	denotes	then
T4310	14576-14589	VBN	denotes	pre-incubated
T4313	14590-14593	IN	denotes	for
T4314	14594-14596	CD	denotes	24
T4315	14597-14598	NN	denotes	h
T4316	14599-14601	IN	denotes	in
T4317	14602-14608	NN	denotes	medium
T4318	14609-14619	VBG	denotes	containing
T4319	14620-14621	CD	denotes	0
T4321	14621-14623	,	denotes	,
T4322	14623-14624	CD	denotes	5
T4323	14624-14626	,	denotes	,
T4324	14626-14628	CD	denotes	10
T4325	14628-14630	,	denotes	,
T4326	14630-14632	CD	denotes	25
T4327	14633-14635	CC	denotes	or
T4328	14636-14638	CD	denotes	50
T4320	14639-14641	NN	denotes	µM
T4329	14642-14643	NN	denotes	l
T4331	14643-14644	HYPH	denotes	-
T4332	14644-14654	NN	denotes	buthionine
T4333	14654-14655	HYPH	denotes	-
T4334	14655-14656	-LRB-	denotes	[
T4335	14656-14657	NN	denotes	S
T4336	14657-14658	,	denotes	,
T4337	14658-14659	NN	denotes	R
T4338	14659-14660	-RRB-	denotes	]
T4339	14660-14661	HYPH	denotes	-
T4330	14661-14672	NN	denotes	sulfoximine
T4340	14673-14674	-LRB-	denotes	(
T4341	14674-14677	NN	denotes	BSO
T4342	14677-14678	-RRB-	denotes	)
T4343	14679-14680	-LRB-	denotes	(
T4344	14680-14685	NNP	denotes	Sigma
T4345	14685-14686	-RRB-	denotes	)
T4346	14686-14688	,	denotes	,
T4347	14688-14689	DT	denotes	a
T4348	14690-14694	NN	denotes	drug
T4349	14695-14699	WDT	denotes	that
T4350	14700-14708	VBZ	denotes	inhibits
T4351	14709-14720	NN	denotes	glutathione
T4352	14721-14730	NN	denotes	synthesis
T4353	14731-14732	-LRB-	denotes	(
T4354	14732-14734	CD	denotes	31
T4355	14734-14735	-RRB-	denotes	)
T4356	14735-14736	.	denotes	.
T4357	14736-14852	sentence	denotes	Later, cells were exposed to 0, 5, 10 or 20 µM CdCl2 in the specified pre-incubation medium for an additional 24 h.
T4358	14737-14742	RB	denotes	Later
T4360	14742-14744	,	denotes	,
T4361	14744-14749	NNS	denotes	cells
T4362	14750-14754	VBD	denotes	were
T4359	14755-14762	VBN	denotes	exposed
T4363	14763-14765	IN	denotes	to
T4364	14766-14767	CD	denotes	0
T4366	14767-14769	,	denotes	,
T4367	14769-14770	CD	denotes	5
T4368	14770-14772	,	denotes	,
T4369	14772-14774	CD	denotes	10
T4370	14775-14777	CC	denotes	or
T4371	14778-14780	CD	denotes	20
T4365	14781-14783	NN	denotes	µM
T4372	14784-14789	NN	denotes	CdCl2
T4373	14790-14792	IN	denotes	in
T4374	14793-14796	DT	denotes	the
T4376	14797-14806	VBN	denotes	specified
T4377	14807-14821	NN	denotes	pre-incubation
T4375	14822-14828	NN	denotes	medium
T4378	14829-14832	IN	denotes	for
T4379	14833-14835	DT	denotes	an
T4381	14836-14846	JJ	denotes	additional
T4382	14847-14849	CD	denotes	24
T4380	14850-14851	NN	denotes	h
T4383	14851-14852	.	denotes	.
T4384	14852-15039	sentence	denotes	Cytotoxicity was determined by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromid)-based Cell Proliferation Kit I (Roche) according to the manufacturer's instructions.
T4385	14853-14865	NN	denotes	Cytotoxicity
T4387	14866-14869	VBD	denotes	was
T4386	14870-14880	VBN	denotes	determined
T4388	14881-14883	IN	denotes	by
T4389	14884-14887	DT	denotes	the
T4391	14888-14891	NN	denotes	MTT
T4393	14892-14893	-LRB-	denotes	(
T4395	14893-14894	CD	denotes	3
T4397	14894-14895	HYPH	denotes	-
T4398	14895-14896	-LRB-	denotes	[
T4399	14896-14897	CD	denotes	4
T4400	14897-14898	,	denotes	,
T4401	14898-14899	CD	denotes	5
T4402	14899-14900	HYPH	denotes	-
T4396	14900-14915	NN	denotes	dimethylthiazol
T4403	14915-14916	HYPH	denotes	-
T4404	14916-14917	CD	denotes	2
T4406	14917-14918	HYPH	denotes	-
T4405	14918-14920	NN	denotes	yl
T4407	14920-14921	-RRB-	denotes	]
T4408	14921-14922	HYPH	denotes	-
T4409	14922-14923	CD	denotes	2
T4410	14923-14924	,	denotes	,
T4411	14924-14925	CD	denotes	5
T4413	14925-14926	HYPH	denotes	-
T4412	14926-14934	NN	denotes	diphenyl
T4414	14935-14946	NN	denotes	tetrazolium
T4394	14947-14953	NN	denotes	bromid
T4415	14953-14954	-RRB-	denotes	)
T4416	14954-14955	HYPH	denotes	-
T4392	14955-14960	VBN	denotes	based
T4417	14961-14965	NNP	denotes	Cell
T4418	14966-14979	NNP	denotes	Proliferation
T4390	14980-14983	NNP	denotes	Kit
T4419	14984-14985	CD	denotes	I
T4420	14986-14987	-LRB-	denotes	(
T4421	14987-14992	NNP	denotes	Roche
T4422	14992-14993	-RRB-	denotes	)
T4423	14994-15003	VBG	denotes	according
T4424	15004-15006	IN	denotes	to
T4425	15007-15010	DT	denotes	the
T4426	15011-15023	NN	denotes	manufacturer
T4428	15023-15025	POS	denotes	's
T4427	15026-15038	NNS	denotes	instructions
T4429	15038-15039	.	denotes	.
T4679	15050-15060	NN	denotes	Generation
T4680	15061-15063	IN	denotes	of
T4681	15064-15066	DT	denotes	an
T4683	15067-15076	JJ	denotes	inducible
T4684	15076-15078	,	denotes	,
T4685	15078-15083	NN	denotes	liver
T4687	15083-15084	HYPH	denotes	-
T4686	15084-15092	JJ	denotes	specific
T4688	15093-15097	NN	denotes	Mtf1
T4689	15098-15106	NN	denotes	knockout
T4690	15107-15112	NN	denotes	mouse
T4682	15113-15117	NN	denotes	line
T4691	15117-15341	sentence	denotes	Using a homologous recombination strategy, mice were obtained with a modified Mtf1 allele Mtf1loxP where exons 3 and 4, encoding four of the six zinc fingers of the DNA-binding domain, are flanked by loxP sites (Figure 1a).
T4692	15118-15123	VBG	denotes	Using
T4694	15124-15125	DT	denotes	a
T4696	15126-15136	JJ	denotes	homologous
T4697	15137-15150	NN	denotes	recombination
T4695	15151-15159	NN	denotes	strategy
T4698	15159-15161	,	denotes	,
T4699	15161-15165	NNS	denotes	mice
T4700	15166-15170	VBD	denotes	were
T4693	15171-15179	VBN	denotes	obtained
T4701	15180-15184	IN	denotes	with
T4702	15185-15186	DT	denotes	a
T4704	15187-15195	VBN	denotes	modified
T4705	15196-15200	NN	denotes	Mtf1
T4706	15201-15207	NN	denotes	allele
T4703	15208-15216	NN	denotes	Mtf1loxP
T4707	15217-15222	WRB	denotes	where
T4709	15223-15228	NNS	denotes	exons
T4710	15229-15230	CD	denotes	3
T4711	15231-15234	CC	denotes	and
T4712	15235-15236	CD	denotes	4
T4713	15236-15238	,	denotes	,
T4714	15238-15246	VBG	denotes	encoding
T4715	15247-15251	CD	denotes	four
T4716	15252-15254	IN	denotes	of
T4717	15255-15258	DT	denotes	the
T4719	15259-15262	CD	denotes	six
T4720	15263-15267	NN	denotes	zinc
T4718	15268-15275	NNS	denotes	fingers
T4721	15276-15278	IN	denotes	of
T4722	15279-15282	DT	denotes	the
T4724	15283-15286	NN	denotes	DNA
T4726	15286-15287	HYPH	denotes	-
T4725	15287-15294	VBG	denotes	binding
T4723	15295-15301	NN	denotes	domain
T4727	15301-15303	,	denotes	,
T4728	15303-15306	VBP	denotes	are
T4708	15307-15314	VBN	denotes	flanked
T4729	15315-15317	IN	denotes	by
T4730	15318-15322	NN	denotes	loxP
T4731	15323-15328	NNS	denotes	sites
T4732	15329-15330	-LRB-	denotes	(
T4734	15330-15336	NN	denotes	Figure
T4733	15337-15339	NN	denotes	1a
T4735	15339-15340	-RRB-	denotes	)
T4736	15340-15341	.	denotes	.
T4737	15341-15462	sentence	denotes	Mice homozygous for the Mtf1loxP allele were further crossed with animals of the Cre recombinase transgenic line Mx-cre.
T4738	15342-15346	NNS	denotes	Mice
T4740	15347-15357	JJ	denotes	homozygous
T4741	15358-15361	IN	denotes	for
T4742	15362-15365	DT	denotes	the
T4744	15366-15374	NN	denotes	Mtf1loxP
T4743	15375-15381	NN	denotes	allele
T4745	15382-15386	VBD	denotes	were
T4746	15387-15394	RB	denotes	further
T4739	15395-15402	VBN	denotes	crossed
T4747	15403-15407	IN	denotes	with
T4748	15408-15415	NNS	denotes	animals
T4749	15416-15418	IN	denotes	of
T4750	15419-15422	DT	denotes	the
T4752	15423-15426	NN	denotes	Cre
T4753	15427-15438	NN	denotes	recombinase
T4754	15439-15449	JJ	denotes	transgenic
T4751	15450-15454	NN	denotes	line
T4755	15455-15457	NN	denotes	Mx
T4757	15457-15458	HYPH	denotes	-
T4756	15458-15461	NN	denotes	cre
T4758	15461-15462	.	denotes	.
T4759	15462-15665	sentence	denotes	Cre recombinase is expressed in this line under the control of the mouse Mx1 gene promoter, which is inducible by administration of interferon alpha or beta, or synthetic double-stranded RNA pI–pC (32).
T4760	15463-15466	NN	denotes	Cre
T4761	15467-15478	NN	denotes	recombinase
T4763	15479-15481	VBZ	denotes	is
T4762	15482-15491	VBN	denotes	expressed
T4764	15492-15494	IN	denotes	in
T4765	15495-15499	DT	denotes	this
T4766	15500-15504	NN	denotes	line
T4767	15505-15510	IN	denotes	under
T4768	15511-15514	DT	denotes	the
T4769	15515-15522	NN	denotes	control
T4770	15523-15525	IN	denotes	of
T4771	15526-15529	DT	denotes	the
T4773	15530-15535	NN	denotes	mouse
T4774	15536-15539	NN	denotes	Mx1
T4775	15540-15544	NN	denotes	gene
T4772	15545-15553	NN	denotes	promoter
T4776	15553-15555	,	denotes	,
T4777	15555-15560	WDT	denotes	which
T4778	15561-15563	VBZ	denotes	is
T4779	15564-15573	JJ	denotes	inducible
T4780	15574-15576	IN	denotes	by
T4781	15577-15591	NN	denotes	administration
T4782	15592-15594	IN	denotes	of
T4783	15595-15605	NN	denotes	interferon
T4784	15606-15611	NN	denotes	alpha
T4785	15612-15614	CC	denotes	or
T4786	15615-15619	NN	denotes	beta
T4787	15619-15621	,	denotes	,
T4788	15621-15623	CC	denotes	or
T4789	15624-15633	JJ	denotes	synthetic
T4791	15634-15640	JJ	denotes	double
T4793	15640-15641	HYPH	denotes	-
T4792	15641-15649	VBN	denotes	stranded
T4794	15650-15653	NN	denotes	RNA
T4795	15654-15656	NN	denotes	pI
T4796	15656-15657	HYPH	denotes	–
T4790	15657-15659	NN	denotes	pC
T4797	15660-15661	-LRB-	denotes	(
T4798	15661-15663	CD	denotes	32
T4799	15663-15664	-RRB-	denotes	)
T4800	15664-15665	.	denotes	.
T4801	15665-15809	sentence	denotes	Cre-mediated deletion was reported to be complete in the liver, while varying in other tissues, ranging from 94% in spleen to 8% in brain (32).
T4802	15666-15669	NN	denotes	Cre
T4804	15669-15670	HYPH	denotes	-
T4803	15670-15678	VBN	denotes	mediated
T4805	15679-15687	NN	denotes	deletion
T4807	15688-15691	VBD	denotes	was
T4806	15692-15700	VBN	denotes	reported
T4808	15701-15703	TO	denotes	to
T4809	15704-15706	VB	denotes	be
T4810	15707-15715	JJ	denotes	complete
T4811	15716-15718	IN	denotes	in
T4812	15719-15722	DT	denotes	the
T4813	15723-15728	NN	denotes	liver
T4814	15728-15730	,	denotes	,
T4815	15730-15735	IN	denotes	while
T4816	15736-15743	VBG	denotes	varying
T4817	15744-15746	IN	denotes	in
T4818	15747-15752	JJ	denotes	other
T4819	15753-15760	NNS	denotes	tissues
T4820	15760-15762	,	denotes	,
T4821	15762-15769	VBG	denotes	ranging
T4822	15770-15774	IN	denotes	from
T4823	15775-15777	CD	denotes	94
T4824	15777-15778	NN	denotes	%
T4825	15779-15781	IN	denotes	in
T4826	15782-15788	NN	denotes	spleen
T4827	15789-15791	IN	denotes	to
T4828	15792-15793	CD	denotes	8
T4829	15793-15794	NN	denotes	%
T4830	15795-15797	IN	denotes	in
T4831	15798-15803	NN	denotes	brain
T4832	15804-15805	-LRB-	denotes	(
T4833	15805-15807	CD	denotes	32
T4834	15807-15808	-RRB-	denotes	)
T4835	15808-15809	.	denotes	.
T4836	15809-15962	sentence	denotes	After Cre-mediated deletion of exons 3 and 4, which results in a frameshift and premature translation stop, no functional MTF-1 protein can be produced.
T4837	15810-15815	IN	denotes	After
T4839	15816-15819	NN	denotes	Cre
T4841	15819-15820	HYPH	denotes	-
T4840	15820-15828	VBN	denotes	mediated
T4842	15829-15837	NN	denotes	deletion
T4843	15838-15840	IN	denotes	of
T4844	15841-15846	NNS	denotes	exons
T4845	15847-15848	CD	denotes	3
T4846	15849-15852	CC	denotes	and
T4847	15853-15854	CD	denotes	4
T4848	15854-15856	,	denotes	,
T4849	15856-15861	WDT	denotes	which
T4850	15862-15869	VBZ	denotes	results
T4851	15870-15872	IN	denotes	in
T4852	15873-15874	DT	denotes	a
T4854	15875-15885	NN	denotes	frameshift
T4855	15886-15889	CC	denotes	and
T4856	15890-15899	JJ	denotes	premature
T4857	15900-15911	NN	denotes	translation
T4853	15912-15916	NN	denotes	stop
T4858	15916-15918	,	denotes	,
T4859	15918-15920	DT	denotes	no
T4861	15921-15931	JJ	denotes	functional
T4862	15932-15935	NN	denotes	MTF
T4863	15935-15936	HYPH	denotes	-
T4864	15936-15937	CD	denotes	1
T4860	15938-15945	NN	denotes	protein
T4865	15946-15949	MD	denotes	can
T4866	15950-15952	VB	denotes	be
T4838	15953-15961	VBN	denotes	produced
T4867	15961-15962	.	denotes	.
T4868	15962-16234	sentence	denotes	For induction of Cre recombinase, Mtf1 conditional knockout mice harboring the Mx-cre transgene (Mtf1Mx-cre) received four intraperitoneal pI–pC injections at 3 day intervals (pI–pC induction); control littermates without transgene (Mtf1loxP) received similar injections.
T4869	15963-15966	IN	denotes	For
T4871	15967-15976	NN	denotes	induction
T4872	15977-15979	IN	denotes	of
T4873	15980-15983	NN	denotes	Cre
T4874	15984-15995	NN	denotes	recombinase
T4875	15995-15997	,	denotes	,
T4876	15997-16001	NN	denotes	Mtf1
T4878	16002-16013	JJ	denotes	conditional
T4879	16014-16022	NN	denotes	knockout
T4877	16023-16027	NNS	denotes	mice
T4880	16028-16037	VBG	denotes	harboring
T4881	16038-16041	DT	denotes	the
T4883	16042-16044	NN	denotes	Mx
T4885	16044-16045	HYPH	denotes	-
T4884	16045-16048	NN	denotes	cre
T4882	16049-16058	NN	denotes	transgene
T4886	16059-16060	-LRB-	denotes	(
T4887	16060-16066	NN	denotes	Mtf1Mx
T4889	16066-16067	HYPH	denotes	-
T4888	16067-16070	NN	denotes	cre
T4890	16070-16071	-RRB-	denotes	)
T4870	16072-16080	VBD	denotes	received
T4892	16081-16085	CD	denotes	four
T4894	16086-16101	JJ	denotes	intraperitoneal
T4895	16102-16104	NN	denotes	pI
T4897	16104-16105	HYPH	denotes	–
T4896	16105-16107	NN	denotes	pC
T4893	16108-16118	NNS	denotes	injections
T4898	16119-16121	IN	denotes	at
T4899	16122-16123	CD	denotes	3
T4900	16124-16127	NN	denotes	day
T4901	16128-16137	NNS	denotes	intervals
T4902	16138-16139	-LRB-	denotes	(
T4904	16139-16141	NN	denotes	pI
T4906	16141-16142	HYPH	denotes	–
T4905	16142-16144	NN	denotes	pC
T4903	16145-16154	NN	denotes	induction
T4907	16154-16155	-RRB-	denotes	)
T4908	16155-16156	:	denotes	;
T4909	16157-16164	NN	denotes	control
T4910	16165-16176	NNS	denotes	littermates
T4911	16177-16184	IN	denotes	without
T4912	16185-16194	NN	denotes	transgene
T4913	16195-16196	-LRB-	denotes	(
T4914	16196-16204	NN	denotes	Mtf1loxP
T4915	16204-16205	-RRB-	denotes	)
T4891	16206-16214	VBD	denotes	received
T4916	16215-16222	JJ	denotes	similar
T4917	16223-16233	NNS	denotes	injections
T4918	16233-16234	.	denotes	.
T4919	16234-16403	sentence	denotes	Using RT–PCRs (Figure 1b), a shortened product was obtained with RNA from Mtf1Mx-cre livers, indicating a successful excision of exons 3 and 4 of Mtf1 in these animals.
T4920	16235-16240	VBG	denotes	Using
T4922	16241-16243	NN	denotes	RT
T4924	16243-16244	HYPH	denotes	–
T4923	16244-16248	NNS	denotes	PCRs
T4925	16249-16250	-LRB-	denotes	(
T4927	16250-16256	NN	denotes	Figure
T4926	16257-16259	NN	denotes	1b
T4928	16259-16260	-RRB-	denotes	)
T4929	16260-16262	,	denotes	,
T4930	16262-16263	DT	denotes	a
T4932	16264-16273	VBN	denotes	shortened
T4931	16274-16281	NN	denotes	product
T4933	16282-16285	VBD	denotes	was
T4921	16286-16294	VBN	denotes	obtained
T4934	16295-16299	IN	denotes	with
T4935	16300-16303	NN	denotes	RNA
T4936	16304-16308	IN	denotes	from
T4937	16309-16315	NN	denotes	Mtf1Mx
T4939	16315-16316	HYPH	denotes	-
T4938	16316-16319	NN	denotes	cre
T4940	16320-16326	NNS	denotes	livers
T4941	16326-16328	,	denotes	,
T4942	16328-16338	VBG	denotes	indicating
T4943	16339-16340	DT	denotes	a
T4945	16341-16351	JJ	denotes	successful
T4944	16352-16360	NN	denotes	excision
T4946	16361-16363	IN	denotes	of
T4947	16364-16369	NNS	denotes	exons
T4948	16370-16371	CD	denotes	3
T4949	16372-16375	CC	denotes	and
T4950	16376-16377	CD	denotes	4
T4951	16378-16380	IN	denotes	of
T4952	16381-16385	NN	denotes	Mtf1
T4953	16386-16388	IN	denotes	in
T4954	16389-16394	DT	denotes	these
T4955	16395-16402	NNS	denotes	animals
T4956	16402-16403	.	denotes	.
T4957	16403-16578	sentence	denotes	On close examination, a very faint band similar in size to full-length signal was also observed in those mice, probably due to a low amount of residual full-length Mtf1 mRNA.
T4958	16404-16406	IN	denotes	On
T4960	16407-16412	JJ	denotes	close
T4961	16413-16424	NN	denotes	examination
T4962	16424-16426	,	denotes	,
T4963	16426-16427	DT	denotes	a
T4965	16428-16432	RB	denotes	very
T4966	16433-16438	JJ	denotes	faint
T4964	16439-16443	NN	denotes	band
T4967	16444-16451	JJ	denotes	similar
T4968	16452-16454	IN	denotes	in
T4969	16455-16459	NN	denotes	size
T4970	16460-16462	IN	denotes	to
T4971	16463-16467	JJ	denotes	full
T4973	16467-16468	HYPH	denotes	-
T4972	16468-16474	NN	denotes	length
T4974	16475-16481	NN	denotes	signal
T4975	16482-16485	VBD	denotes	was
T4976	16486-16490	RB	denotes	also
T4959	16491-16499	VBN	denotes	observed
T4977	16500-16502	IN	denotes	in
T4978	16503-16508	DT	denotes	those
T4979	16509-16513	NNS	denotes	mice
T4980	16513-16515	,	denotes	,
T4981	16515-16523	RB	denotes	probably
T4982	16524-16527	IN	denotes	due
T4983	16528-16530	IN	denotes	to
T4984	16531-16532	DT	denotes	a
T4986	16533-16536	JJ	denotes	low
T4985	16537-16543	NN	denotes	amount
T4987	16544-16546	IN	denotes	of
T4988	16547-16555	JJ	denotes	residual
T4990	16556-16560	JJ	denotes	full
T4992	16560-16561	HYPH	denotes	-
T4991	16561-16567	NN	denotes	length
T4993	16568-16572	NN	denotes	Mtf1
T4989	16573-16577	NN	denotes	mRNA
T4994	16577-16578	.	denotes	.
T4995	16578-16828	sentence	denotes	The level of functional MTF-1 protein was examined by EMSA (Figure 1c): MTF-1 protein–DNA complex was detectable with liver protein extract from an Mtf1loxP control mouse, but no functional MRE-binding protein was observed with an Mtf1Mx-cre sample.
T4996	16579-16582	DT	denotes	The
T4997	16583-16588	NN	denotes	level
T4999	16589-16591	IN	denotes	of
T5000	16592-16602	JJ	denotes	functional
T5002	16603-16606	NN	denotes	MTF
T5003	16606-16607	HYPH	denotes	-
T5004	16607-16608	CD	denotes	1
T5001	16609-16616	NN	denotes	protein
T5005	16617-16620	VBD	denotes	was
T4998	16621-16629	VBN	denotes	examined
T5007	16630-16632	IN	denotes	by
T5008	16633-16637	NN	denotes	EMSA
T5009	16638-16639	-LRB-	denotes	(
T5011	16639-16645	NN	denotes	Figure
T5010	16646-16648	NN	denotes	1c
T5012	16648-16649	-RRB-	denotes	)
T5013	16649-16651	:	denotes	:
T5014	16651-16654	NN	denotes	MTF
T5016	16654-16655	HYPH	denotes	-
T5017	16655-16656	CD	denotes	1
T5018	16657-16664	NN	denotes	protein
T5019	16664-16665	HYPH	denotes	–
T5020	16665-16668	NN	denotes	DNA
T5015	16669-16676	NN	denotes	complex
T5006	16677-16680	VBD	denotes	was
T5021	16681-16691	JJ	denotes	detectable
T5022	16692-16696	IN	denotes	with
T5023	16697-16702	NN	denotes	liver
T5025	16703-16710	NN	denotes	protein
T5024	16711-16718	NN	denotes	extract
T5026	16719-16723	IN	denotes	from
T5027	16724-16726	DT	denotes	an
T5029	16727-16735	NN	denotes	Mtf1loxP
T5030	16736-16743	NN	denotes	control
T5028	16744-16749	NN	denotes	mouse
T5031	16749-16751	,	denotes	,
T5032	16751-16754	CC	denotes	but
T5033	16755-16757	DT	denotes	no
T5035	16758-16768	JJ	denotes	functional
T5036	16769-16772	NN	denotes	MRE
T5038	16772-16773	HYPH	denotes	-
T5037	16773-16780	VBG	denotes	binding
T5034	16781-16788	NN	denotes	protein
T5040	16789-16792	VBD	denotes	was
T5039	16793-16801	VBN	denotes	observed
T5041	16802-16806	IN	denotes	with
T5042	16807-16809	DT	denotes	an
T5044	16810-16816	NN	denotes	Mtf1Mx
T5046	16816-16817	HYPH	denotes	-
T5045	16817-16820	NN	denotes	cre
T5043	16821-16827	NN	denotes	sample
T5047	16827-16828	.	denotes	.
T5048	16828-16924	sentence	denotes	Thus, deletion of exons 3 and 4 of Mtf1 in the liver of Mtf1Mx-cre mice was virtually complete.
T5049	16829-16833	RB	denotes	Thus
T5051	16833-16835	,	denotes	,
T5052	16835-16843	NN	denotes	deletion
T5053	16844-16846	IN	denotes	of
T5054	16847-16852	NNS	denotes	exons
T5055	16853-16854	CD	denotes	3
T5056	16855-16858	CC	denotes	and
T5057	16859-16860	CD	denotes	4
T5058	16861-16863	IN	denotes	of
T5059	16864-16868	NN	denotes	Mtf1
T5060	16869-16871	IN	denotes	in
T5061	16872-16875	DT	denotes	the
T5062	16876-16881	NN	denotes	liver
T5063	16882-16884	IN	denotes	of
T5064	16885-16891	NN	denotes	Mtf1Mx
T5066	16891-16892	HYPH	denotes	-
T5065	16892-16895	NN	denotes	cre
T5067	16896-16900	NNS	denotes	mice
T5050	16901-16904	VBD	denotes	was
T5068	16905-16914	RB	denotes	virtually
T5069	16915-16923	JJ	denotes	complete
T5070	16923-16924	.	denotes	.
T5071	16924-17027	sentence	denotes	All examined liver-specific knockout mice were viable under laboratory conditions and appeared normal.
T5072	16925-16928	DT	denotes	All
T5074	16929-16937	VBN	denotes	examined
T5075	16938-16943	NN	denotes	liver
T5077	16943-16944	HYPH	denotes	-
T5076	16944-16952	JJ	denotes	specific
T5078	16953-16961	NN	denotes	knockout
T5073	16962-16966	NNS	denotes	mice
T5079	16967-16971	VBD	denotes	were
T5080	16972-16978	JJ	denotes	viable
T5081	16979-16984	IN	denotes	under
T5082	16985-16995	NN	denotes	laboratory
T5083	16996-17006	NNS	denotes	conditions
T5084	17007-17010	CC	denotes	and
T5085	17011-17019	VBD	denotes	appeared
T5086	17020-17026	JJ	denotes	normal
T5087	17026-17027	.	denotes	.
T5337	17029-17032	NN	denotes	MTF
T5339	17032-17033	HYPH	denotes	-
T5340	17033-17034	CD	denotes	1
T5341	17035-17041	NN	denotes	target
T5342	17042-17046	NN	denotes	gene
T5338	17047-17053	NN	denotes	search
T5343	17053-17283	sentence	denotes	For the identification of MTF-1 target genes, we compared the liver transcript profiles of mice with and without functional Mtf1 gene that had been mock-treated or exposed to cadmium (n = 3 per genotype and respective treatment).
T5344	17054-17057	IN	denotes	For
T5346	17058-17061	DT	denotes	the
T5347	17062-17076	NN	denotes	identification
T5348	17077-17079	IN	denotes	of
T5349	17080-17083	NN	denotes	MTF
T5351	17083-17084	HYPH	denotes	-
T5352	17084-17085	CD	denotes	1
T5353	17086-17092	NN	denotes	target
T5350	17093-17098	NNS	denotes	genes
T5354	17098-17100	,	denotes	,
T5355	17100-17102	PRP	denotes	we
T5345	17103-17111	VBD	denotes	compared
T5356	17112-17115	DT	denotes	the
T5358	17116-17121	NN	denotes	liver
T5359	17122-17132	NN	denotes	transcript
T5357	17133-17141	NNS	denotes	profiles
T5360	17142-17144	IN	denotes	of
T5361	17145-17149	NNS	denotes	mice
T5362	17150-17154	IN	denotes	with
T5363	17155-17158	CC	denotes	and
T5364	17159-17166	IN	denotes	without
T5365	17167-17177	JJ	denotes	functional
T5367	17178-17182	NN	denotes	Mtf1
T5366	17183-17187	NN	denotes	gene
T5368	17188-17192	WDT	denotes	that
T5370	17193-17196	VBD	denotes	had
T5371	17197-17201	VBN	denotes	been
T5372	17202-17206	JJ	denotes	mock
T5373	17206-17207	HYPH	denotes	-
T5369	17207-17214	VBN	denotes	treated
T5374	17215-17217	CC	denotes	or
T5375	17218-17225	VBN	denotes	exposed
T5376	17226-17228	IN	denotes	to
T5377	17229-17236	NN	denotes	cadmium
T5378	17237-17238	-LRB-	denotes	(
T5380	17238-17239	NN	denotes	n
T5381	17240-17241	SYM	denotes	=
T5379	17242-17243	CD	denotes	3
T5382	17244-17247	IN	denotes	per
T5383	17248-17256	NN	denotes	genotype
T5384	17257-17260	CC	denotes	and
T5385	17261-17271	JJ	denotes	respective
T5386	17272-17281	NN	denotes	treatment
T5387	17281-17282	-RRB-	denotes	)
T5388	17282-17283	.	denotes	.
T5389	17283-17459	sentence	denotes	In a first screen, the transcripts were analyzed with a differential display-based method, called amplification of double-stranded cDNA end restriction fragments (ADDER) (33).
T5390	17284-17286	IN	denotes	In
T5392	17287-17288	DT	denotes	a
T5394	17289-17294	JJ	denotes	first
T5393	17295-17301	NN	denotes	screen
T5395	17301-17303	,	denotes	,
T5396	17303-17306	DT	denotes	the
T5397	17307-17318	NNS	denotes	transcripts
T5398	17319-17323	VBD	denotes	were
T5391	17324-17332	VBN	denotes	analyzed
T5399	17333-17337	IN	denotes	with
T5400	17338-17339	DT	denotes	a
T5402	17340-17352	JJ	denotes	differential
T5403	17353-17360	NN	denotes	display
T5405	17360-17361	HYPH	denotes	-
T5404	17361-17366	VBN	denotes	based
T5401	17367-17373	NN	denotes	method
T5406	17373-17375	,	denotes	,
T5407	17375-17381	VBN	denotes	called
T5408	17382-17395	NN	denotes	amplification
T5409	17396-17398	IN	denotes	of
T5410	17399-17405	JJ	denotes	double
T5412	17405-17406	HYPH	denotes	-
T5411	17406-17414	VBN	denotes	stranded
T5414	17415-17419	NN	denotes	cDNA
T5415	17420-17423	NN	denotes	end
T5416	17424-17435	NN	denotes	restriction
T5413	17436-17445	NNS	denotes	fragments
T5417	17446-17447	-LRB-	denotes	(
T5418	17447-17452	NN	denotes	ADDER
T5419	17452-17453	-RRB-	denotes	)
T5420	17454-17455	-LRB-	denotes	(
T5421	17455-17457	CD	denotes	33
T5422	17457-17458	-RRB-	denotes	)
T5423	17458-17459	.	denotes	.
T5424	17459-17735	sentence	denotes	Thereby an overwhelming number of signals was obtained for the two stress-inducible metallothioneins (Mt1 and Mt2), due to the abundance of their transcripts both in mock-treated and especially in cadmium-treated livers that harbored a functional MTF-1 gene (data not shown).
T5425	17460-17467	RB	denotes	Thereby
T5427	17468-17470	DT	denotes	an
T5429	17471-17483	JJ	denotes	overwhelming
T5428	17484-17490	NN	denotes	number
T5430	17491-17493	IN	denotes	of
T5431	17494-17501	NNS	denotes	signals
T5432	17502-17505	VBD	denotes	was
T5426	17506-17514	VBN	denotes	obtained
T5433	17515-17518	IN	denotes	for
T5434	17519-17522	DT	denotes	the
T5436	17523-17526	CD	denotes	two
T5437	17527-17533	NN	denotes	stress
T5439	17533-17534	HYPH	denotes	-
T5438	17534-17543	JJ	denotes	inducible
T5435	17544-17560	NNS	denotes	metallothioneins
T5440	17561-17562	-LRB-	denotes	(
T5441	17562-17565	NN	denotes	Mt1
T5442	17566-17569	CC	denotes	and
T5443	17570-17573	NN	denotes	Mt2
T5444	17573-17574	-RRB-	denotes	)
T5445	17574-17576	,	denotes	,
T5446	17576-17579	IN	denotes	due
T5447	17580-17582	IN	denotes	to
T5448	17583-17586	DT	denotes	the
T5449	17587-17596	NN	denotes	abundance
T5450	17597-17599	IN	denotes	of
T5451	17600-17605	PRP$	denotes	their
T5452	17606-17617	NNS	denotes	transcripts
T5453	17618-17622	CC	denotes	both
T5454	17623-17625	IN	denotes	in
T5455	17626-17630	JJ	denotes	mock
T5457	17630-17631	HYPH	denotes	-
T5456	17631-17638	VBN	denotes	treated
T5458	17639-17642	CC	denotes	and
T5459	17643-17653	RB	denotes	especially
T5460	17654-17656	IN	denotes	in
T5461	17657-17664	NN	denotes	cadmium
T5463	17664-17665	HYPH	denotes	-
T5462	17665-17672	VBN	denotes	treated
T5464	17673-17679	NNS	denotes	livers
T5465	17680-17684	WDT	denotes	that
T5466	17685-17693	VBD	denotes	harbored
T5467	17694-17695	DT	denotes	a
T5469	17696-17706	JJ	denotes	functional
T5470	17707-17710	NN	denotes	MTF
T5471	17710-17711	HYPH	denotes	-
T5472	17711-17712	CD	denotes	1
T5468	17713-17717	NN	denotes	gene
T5473	17718-17719	-LRB-	denotes	(
T5475	17719-17723	NNS	denotes	data
T5476	17724-17727	RB	denotes	not
T5474	17728-17733	VBN	denotes	shown
T5477	17733-17734	-RRB-	denotes	)
T5478	17734-17735	.	denotes	.
T5479	17735-17851	sentence	denotes	This result confirmed the importance of MTF-1 for both basal and metal-induced expression of metallothionein genes.
T5480	17736-17740	DT	denotes	This
T5481	17741-17747	NN	denotes	result
T5482	17748-17757	VBD	denotes	confirmed
T5483	17758-17761	DT	denotes	the
T5484	17762-17772	NN	denotes	importance
T5485	17773-17775	IN	denotes	of
T5486	17776-17779	NN	denotes	MTF
T5487	17779-17780	HYPH	denotes	-
T5488	17780-17781	CD	denotes	1
T5489	17782-17785	IN	denotes	for
T5490	17786-17790	CC	denotes	both
T5491	17791-17796	JJ	denotes	basal
T5493	17797-17800	CC	denotes	and
T5494	17801-17806	NN	denotes	metal
T5496	17806-17807	HYPH	denotes	-
T5495	17807-17814	VBN	denotes	induced
T5492	17815-17825	NN	denotes	expression
T5497	17826-17828	IN	denotes	of
T5498	17829-17844	NN	denotes	metallothionein
T5499	17845-17850	NNS	denotes	genes
T5500	17850-17851	.	denotes	.
T5501	17851-18015	sentence	denotes	In a second approach, the gene expression profile in livers of the above mentioned mice was compared by Affymetrix GeneChip® Mouse Genome 430 2.0 Arrays (Table 1).
T5502	17852-17854	IN	denotes	In
T5504	17855-17856	DT	denotes	a
T5506	17857-17863	JJ	denotes	second
T5505	17864-17872	NN	denotes	approach
T5507	17872-17874	,	denotes	,
T5508	17874-17877	DT	denotes	the
T5510	17878-17882	NN	denotes	gene
T5511	17883-17893	NN	denotes	expression
T5509	17894-17901	NN	denotes	profile
T5512	17902-17904	IN	denotes	in
T5513	17905-17911	NNS	denotes	livers
T5514	17912-17914	IN	denotes	of
T5515	17915-17918	DT	denotes	the
T5517	17919-17924	RB	denotes	above
T5518	17925-17934	VBN	denotes	mentioned
T5516	17935-17939	NNS	denotes	mice
T5519	17940-17943	VBD	denotes	was
T5503	17944-17952	VBN	denotes	compared
T5520	17953-17955	IN	denotes	by
T5521	17956-17966	NNP	denotes	Affymetrix
T5522	17967-17975	NNP	denotes	GeneChip
T5524	17975-17976	SYM	denotes	®
T5525	17977-17982	NNP	denotes	Mouse
T5526	17983-17989	NNP	denotes	Genome
T5527	17990-17993	CD	denotes	430
T5528	17994-17997	CD	denotes	2.0
T5523	17998-18004	NNS	denotes	Arrays
T5529	18005-18006	-LRB-	denotes	(
T5530	18006-18011	NN	denotes	Table
T5531	18012-18013	CD	denotes	1
T5532	18013-18014	-RRB-	denotes	)
T5533	18014-18015	.	denotes	.
T5534	18015-18294	sentence	denotes	When analyzing the probe array data of livers from mock-treated Mtf1Mx-cre and Mtf1loxP mice, an at least 2-fold, reliable downregulation of expression was detected in Mtf1Mx-cre livers for 13 Affymetrix GeneChip® probe sets corresponding to 11 characterized genes (Table 1, a).
T5535	18016-18020	WRB	denotes	When
T5536	18021-18030	VBG	denotes	analyzing
T5538	18031-18034	DT	denotes	the
T5540	18035-18040	NN	denotes	probe
T5541	18041-18046	NN	denotes	array
T5539	18047-18051	NNS	denotes	data
T5542	18052-18054	IN	denotes	of
T5543	18055-18061	NNS	denotes	livers
T5544	18062-18066	IN	denotes	from
T5545	18067-18071	JJ	denotes	mock
T5547	18071-18072	HYPH	denotes	-
T5546	18072-18079	VBN	denotes	treated
T5549	18080-18086	NN	denotes	Mtf1Mx
T5551	18086-18087	HYPH	denotes	-
T5550	18087-18090	NN	denotes	cre
T5552	18091-18094	CC	denotes	and
T5553	18095-18103	NN	denotes	Mtf1loxP
T5548	18104-18108	NNS	denotes	mice
T5554	18108-18110	,	denotes	,
T5555	18110-18112	DT	denotes	an
T5557	18113-18115	RB	denotes	at
T5558	18116-18121	RBS	denotes	least
T5559	18122-18128	JJ	denotes	2-fold
T5560	18128-18130	,	denotes	,
T5561	18130-18138	JJ	denotes	reliable
T5556	18139-18153	NN	denotes	downregulation
T5562	18154-18156	IN	denotes	of
T5563	18157-18167	NN	denotes	expression
T5564	18168-18171	VBD	denotes	was
T5537	18172-18180	VBN	denotes	detected
T5565	18181-18183	IN	denotes	in
T5566	18184-18190	NN	denotes	Mtf1Mx
T5568	18190-18191	HYPH	denotes	-
T5567	18191-18194	NN	denotes	cre
T5569	18195-18201	NNS	denotes	livers
T5570	18202-18205	IN	denotes	for
T5571	18206-18208	CD	denotes	13
T5573	18209-18219	NNP	denotes	Affymetrix
T5574	18220-18228	NNP	denotes	GeneChip
T5575	18228-18229	SYM	denotes	®
T5576	18230-18235	NN	denotes	probe
T5572	18236-18240	NNS	denotes	sets
T5577	18241-18254	VBG	denotes	corresponding
T5578	18255-18257	IN	denotes	to
T5579	18258-18260	CD	denotes	11
T5581	18261-18274	VBN	denotes	characterized
T5580	18275-18280	NNS	denotes	genes
T5582	18281-18282	-LRB-	denotes	(
T5584	18282-18287	NN	denotes	Table
T5585	18288-18289	CD	denotes	1
T5586	18289-18291	,	denotes	,
T5583	18291-18292	NN	denotes	a
T5587	18292-18293	-RRB-	denotes	)
T5588	18293-18294	.	denotes	.
T5589	18294-18436	sentence	denotes	Seven of these genes contain one or more MRE core consensus sequence TGCRCNC within a segment of 1000 bp upstream of the transcription start.
T5590	18295-18300	CD	denotes	Seven
T5592	18301-18303	IN	denotes	of
T5593	18304-18309	DT	denotes	these
T5594	18310-18315	NNS	denotes	genes
T5591	18316-18323	VBP	denotes	contain
T5595	18324-18327	CD	denotes	one
T5597	18328-18330	CC	denotes	or
T5598	18331-18335	JJR	denotes	more
T5599	18336-18339	NN	denotes	MRE
T5600	18340-18344	NN	denotes	core
T5601	18345-18354	NN	denotes	consensus
T5602	18355-18363	NN	denotes	sequence
T5596	18364-18371	NN	denotes	TGCRCNC
T5603	18372-18378	IN	denotes	within
T5604	18379-18380	DT	denotes	a
T5605	18381-18388	NN	denotes	segment
T5606	18389-18391	IN	denotes	of
T5607	18392-18396	CD	denotes	1000
T5608	18397-18399	NN	denotes	bp
T5609	18400-18408	RB	denotes	upstream
T5610	18409-18411	IN	denotes	of
T5611	18412-18415	DT	denotes	the
T5613	18416-18429	NN	denotes	transcription
T5612	18430-18435	NN	denotes	start
T5614	18435-18436	.	denotes	.
T5615	18436-18677	sentence	denotes	For 26 probe sets corresponding to 24 different characterized genes, a 2-fold or higher, reliable upregulation was detected in Mtf1Mx-cre livers (Table 1, b); 17 of these 24 genes contain MRE core consensus sequences in the upstream region.
T5616	18437-18440	IN	denotes	For
T5618	18441-18443	CD	denotes	26
T5620	18444-18449	NN	denotes	probe
T5619	18450-18454	NNS	denotes	sets
T5621	18455-18468	VBG	denotes	corresponding
T5622	18469-18471	IN	denotes	to
T5623	18472-18474	CD	denotes	24
T5625	18475-18484	JJ	denotes	different
T5626	18485-18498	VBN	denotes	characterized
T5624	18499-18504	NNS	denotes	genes
T5627	18504-18506	,	denotes	,
T5628	18506-18507	DT	denotes	a
T5630	18508-18514	JJ	denotes	2-fold
T5631	18515-18517	CC	denotes	or
T5632	18518-18524	JJR	denotes	higher
T5633	18524-18526	,	denotes	,
T5634	18526-18534	JJ	denotes	reliable
T5629	18535-18547	NN	denotes	upregulation
T5635	18548-18551	VBD	denotes	was
T5617	18552-18560	VBN	denotes	detected
T5637	18561-18563	IN	denotes	in
T5638	18564-18570	NN	denotes	Mtf1Mx
T5640	18570-18571	HYPH	denotes	-
T5639	18571-18574	NN	denotes	cre
T5641	18575-18581	NNS	denotes	livers
T5642	18582-18583	-LRB-	denotes	(
T5644	18583-18588	NN	denotes	Table
T5645	18589-18590	CD	denotes	1
T5646	18590-18592	,	denotes	,
T5643	18592-18593	NN	denotes	b
T5647	18593-18594	-RRB-	denotes	)
T5648	18594-18595	:	denotes	;
T5649	18596-18598	CD	denotes	17
T5650	18599-18601	IN	denotes	of
T5651	18602-18607	DT	denotes	these
T5653	18608-18610	CD	denotes	24
T5652	18611-18616	NNS	denotes	genes
T5636	18617-18624	VBP	denotes	contain
T5654	18625-18628	NN	denotes	MRE
T5655	18629-18633	NN	denotes	core
T5657	18634-18643	NN	denotes	consensus
T5656	18644-18653	NNS	denotes	sequences
T5658	18654-18656	IN	denotes	in
T5659	18657-18660	DT	denotes	the
T5661	18661-18669	JJ	denotes	upstream
T5660	18670-18676	NN	denotes	region
T5662	18676-18677	.	denotes	.
T5663	18677-18980	sentence	denotes	The data set for livers of cadmium-treated Mtf1Mx-cre and Mtf1loxP mice revealed an at least 2-fold, reliable downregulation in Mtf1Mx-cre livers for 21 probe sets corresponding to 16 different characterized genes (Table 1, c); 10 of these contain MRE core consensus sequences in their upstream region.
T5664	18678-18681	DT	denotes	The
T5666	18682-18686	NNS	denotes	data
T5665	18687-18690	NN	denotes	set
T5668	18691-18694	IN	denotes	for
T5669	18695-18701	NNS	denotes	livers
T5670	18702-18704	IN	denotes	of
T5671	18705-18712	NN	denotes	cadmium
T5673	18712-18713	HYPH	denotes	-
T5672	18713-18720	VBN	denotes	treated
T5675	18721-18727	NN	denotes	Mtf1Mx
T5677	18727-18728	HYPH	denotes	-
T5676	18728-18731	NN	denotes	cre
T5678	18732-18735	CC	denotes	and
T5679	18736-18744	NN	denotes	Mtf1loxP
T5674	18745-18749	NNS	denotes	mice
T5667	18750-18758	VBD	denotes	revealed
T5681	18759-18761	DT	denotes	an
T5683	18762-18764	RB	denotes	at
T5684	18765-18770	RBS	denotes	least
T5685	18771-18777	JJ	denotes	2-fold
T5686	18777-18779	,	denotes	,
T5687	18779-18787	JJ	denotes	reliable
T5682	18788-18802	NN	denotes	downregulation
T5688	18803-18805	IN	denotes	in
T5689	18806-18812	NN	denotes	Mtf1Mx
T5691	18812-18813	HYPH	denotes	-
T5690	18813-18816	NN	denotes	cre
T5692	18817-18823	NNS	denotes	livers
T5693	18824-18827	IN	denotes	for
T5694	18828-18830	CD	denotes	21
T5696	18831-18836	NN	denotes	probe
T5695	18837-18841	NNS	denotes	sets
T5697	18842-18855	VBG	denotes	corresponding
T5698	18856-18858	IN	denotes	to
T5699	18859-18861	CD	denotes	16
T5701	18862-18871	JJ	denotes	different
T5702	18872-18885	VBN	denotes	characterized
T5700	18886-18891	NNS	denotes	genes
T5703	18892-18893	-LRB-	denotes	(
T5705	18893-18898	NN	denotes	Table
T5706	18899-18900	CD	denotes	1
T5707	18900-18902	,	denotes	,
T5704	18902-18903	NN	denotes	c
T5708	18903-18904	-RRB-	denotes	)
T5709	18904-18905	:	denotes	;
T5710	18906-18908	CD	denotes	10
T5711	18909-18911	IN	denotes	of
T5712	18912-18917	DT	denotes	these
T5680	18918-18925	VBP	denotes	contain
T5713	18926-18929	NN	denotes	MRE
T5715	18930-18934	NN	denotes	core
T5716	18935-18944	NN	denotes	consensus
T5714	18945-18954	NNS	denotes	sequences
T5717	18955-18957	IN	denotes	in
T5718	18958-18963	PRP$	denotes	their
T5720	18964-18972	JJ	denotes	upstream
T5719	18973-18979	NN	denotes	region
T5721	18979-18980	.	denotes	.
T5722	18980-19149	sentence	denotes	For 9 probe sets corresponding to 9 different characterized genes, an at least 2-fold, reliable upregulation was detected (Table 1, d); five of them contain MRE motifs.
T5723	18981-18984	IN	denotes	For
T5725	18985-18986	CD	denotes	9
T5727	18987-18992	NN	denotes	probe
T5726	18993-18997	NNS	denotes	sets
T5728	18998-19011	VBG	denotes	corresponding
T5729	19012-19014	IN	denotes	to
T5730	19015-19016	CD	denotes	9
T5732	19017-19026	JJ	denotes	different
T5733	19027-19040	VBN	denotes	characterized
T5731	19041-19046	NNS	denotes	genes
T5734	19046-19048	,	denotes	,
T5735	19048-19050	DT	denotes	an
T5737	19051-19053	RB	denotes	at
T5738	19054-19059	RBS	denotes	least
T5739	19060-19066	JJ	denotes	2-fold
T5740	19066-19068	,	denotes	,
T5741	19068-19076	JJ	denotes	reliable
T5736	19077-19089	NN	denotes	upregulation
T5742	19090-19093	VBD	denotes	was
T5724	19094-19102	VBN	denotes	detected
T5744	19103-19104	-LRB-	denotes	(
T5746	19104-19109	NN	denotes	Table
T5747	19110-19111	CD	denotes	1
T5748	19111-19113	,	denotes	,
T5745	19113-19114	NN	denotes	d
T5749	19114-19115	-RRB-	denotes	)
T5750	19115-19116	:	denotes	;
T5751	19117-19121	CD	denotes	five
T5752	19122-19124	IN	denotes	of
T5753	19125-19129	PRP	denotes	them
T5743	19130-19137	VBP	denotes	contain
T5754	19138-19141	NN	denotes	MRE
T5755	19142-19148	NNS	denotes	motifs
T5756	19148-19149	.	denotes	.
T5757	19149-19339	sentence	denotes	In addition to characterized genes, ESTs and RIKEN cDNA sequences were also found in the comparison of Mtf1Mx-cre and Mtf1loxP livers to be differentially expressed (Supplementary Table 1).
T5758	19150-19152	IN	denotes	In
T5760	19153-19161	NN	denotes	addition
T5761	19162-19164	IN	denotes	to
T5762	19165-19178	VBN	denotes	characterized
T5763	19179-19184	NNS	denotes	genes
T5764	19184-19186	,	denotes	,
T5765	19186-19190	NNS	denotes	ESTs
T5766	19191-19194	CC	denotes	and
T5767	19195-19200	NN	denotes	RIKEN
T5768	19201-19205	NN	denotes	cDNA
T5769	19206-19215	NNS	denotes	sequences
T5770	19216-19220	VBD	denotes	were
T5771	19221-19225	RB	denotes	also
T5759	19226-19231	VBN	denotes	found
T5772	19232-19234	IN	denotes	in
T5773	19235-19238	DT	denotes	the
T5774	19239-19249	NN	denotes	comparison
T5775	19250-19252	IN	denotes	of
T5776	19253-19259	NN	denotes	Mtf1Mx
T5778	19259-19260	HYPH	denotes	-
T5777	19260-19263	NN	denotes	cre
T5780	19264-19267	CC	denotes	and
T5781	19268-19276	NN	denotes	Mtf1loxP
T5779	19277-19283	NNS	denotes	livers
T5782	19284-19286	TO	denotes	to
T5784	19287-19289	VB	denotes	be
T5785	19290-19304	RB	denotes	differentially
T5783	19305-19314	VBN	denotes	expressed
T5786	19315-19316	-LRB-	denotes	(
T5788	19316-19329	JJ	denotes	Supplementary
T5787	19330-19335	NN	denotes	Table
T5789	19336-19337	CD	denotes	1
T5790	19337-19338	-RRB-	denotes	)
T5791	19338-19339	.	denotes	.
T5792	19339-19544	sentence	denotes	Downregulation of Mt1 and Mt2 was detected in Mtf1Mx-cre livers for both conditions (though the level of significance for the downregulation of Mt1 in mock-treated animals was above 0.05; data not shown).
T5793	19340-19354	NN	denotes	Downregulation
T5795	19355-19357	IN	denotes	of
T5796	19358-19361	NN	denotes	Mt1
T5797	19362-19365	CC	denotes	and
T5798	19366-19369	NN	denotes	Mt2
T5799	19370-19373	VBD	denotes	was
T5794	19374-19382	VBN	denotes	detected
T5800	19383-19385	IN	denotes	in
T5801	19386-19392	NN	denotes	Mtf1Mx
T5803	19392-19393	HYPH	denotes	-
T5802	19393-19396	NN	denotes	cre
T5804	19397-19403	NNS	denotes	livers
T5805	19404-19407	IN	denotes	for
T5806	19408-19412	DT	denotes	both
T5807	19413-19423	NNS	denotes	conditions
T5808	19424-19425	-LRB-	denotes	(
T5809	19425-19431	IN	denotes	though
T5811	19432-19435	DT	denotes	the
T5812	19436-19441	NN	denotes	level
T5813	19442-19444	IN	denotes	of
T5814	19445-19457	NN	denotes	significance
T5815	19458-19461	IN	denotes	for
T5816	19462-19465	DT	denotes	the
T5817	19466-19480	NN	denotes	downregulation
T5818	19481-19483	IN	denotes	of
T5819	19484-19487	NN	denotes	Mt1
T5820	19488-19490	IN	denotes	in
T5821	19491-19495	JJ	denotes	mock
T5823	19495-19496	HYPH	denotes	-
T5822	19496-19503	VBN	denotes	treated
T5824	19504-19511	NNS	denotes	animals
T5810	19512-19515	VBD	denotes	was
T5825	19516-19521	IN	denotes	above
T5826	19522-19526	CD	denotes	0.05
T5827	19526-19527	:	denotes	;
T5828	19528-19532	NNS	denotes	data
T5830	19533-19536	RB	denotes	not
T5829	19537-19542	VBN	denotes	shown
T5831	19542-19543	-RRB-	denotes	)
T5832	19543-19544	.	denotes	.
T5833	19544-19759	sentence	denotes	For all MTF-1 target genes characterized so far, such as Mt1, Mt2 and Znt1, MTF-1 exerts its transcriptional activation activity via standard MRE sequences located proximal to the transcription start (4,5,8,18,19).
T5834	19545-19548	IN	denotes	For
T5836	19549-19552	DT	denotes	all
T5838	19553-19556	NN	denotes	MTF
T5839	19556-19557	HYPH	denotes	-
T5840	19557-19558	CD	denotes	1
T5841	19559-19565	NN	denotes	target
T5837	19566-19571	NNS	denotes	genes
T5842	19572-19585	VBN	denotes	characterized
T5843	19586-19588	RB	denotes	so
T5844	19589-19592	RB	denotes	far
T5845	19592-19594	,	denotes	,
T5846	19594-19598	JJ	denotes	such
T5847	19599-19601	IN	denotes	as
T5848	19602-19605	NN	denotes	Mt1
T5849	19605-19607	,	denotes	,
T5850	19607-19610	NN	denotes	Mt2
T5851	19611-19614	CC	denotes	and
T5852	19615-19619	NN	denotes	Znt1
T5853	19619-19621	,	denotes	,
T5854	19621-19624	NN	denotes	MTF
T5855	19624-19625	HYPH	denotes	-
T5856	19625-19626	CD	denotes	1
T5835	19627-19633	VBZ	denotes	exerts
T5857	19634-19637	PRP$	denotes	its
T5859	19638-19653	JJ	denotes	transcriptional
T5860	19654-19664	NN	denotes	activation
T5858	19665-19673	NN	denotes	activity
T5861	19674-19677	IN	denotes	via
T5862	19678-19686	JJ	denotes	standard
T5864	19687-19690	NN	denotes	MRE
T5863	19691-19700	NNS	denotes	sequences
T5865	19701-19708	VBN	denotes	located
T5866	19709-19717	RB	denotes	proximal
T5867	19718-19720	IN	denotes	to
T5868	19721-19724	DT	denotes	the
T5870	19725-19738	NN	denotes	transcription
T5869	19739-19744	NN	denotes	start
T5871	19745-19746	-LRB-	denotes	(
T5873	19746-19747	CD	denotes	4
T5874	19747-19748	,	denotes	,
T5875	19748-19749	CD	denotes	5
T5876	19749-19750	,	denotes	,
T5877	19750-19751	CD	denotes	8
T5878	19751-19752	,	denotes	,
T5879	19752-19754	CD	denotes	18
T5880	19754-19755	,	denotes	,
T5872	19755-19757	CD	denotes	19
T5881	19757-19758	-RRB-	denotes	)
T5882	19758-19759	.	denotes	.
T5883	19759-19964	sentence	denotes	Even a specific search for MTF-1 binding sites by selection from a pool of double-stranded oligonucleotides with random sequences yielded no new binding motif for MTF-1 in addition to the known MREs (34).
T5884	19760-19764	RB	denotes	Even
T5886	19765-19766	DT	denotes	a
T5887	19767-19775	JJ	denotes	specific
T5885	19776-19782	NN	denotes	search
T5889	19783-19786	IN	denotes	for
T5890	19787-19790	NN	denotes	MTF
T5892	19790-19791	HYPH	denotes	-
T5893	19791-19792	CD	denotes	1
T5894	19793-19800	NN	denotes	binding
T5891	19801-19806	NNS	denotes	sites
T5895	19807-19809	IN	denotes	by
T5896	19810-19819	NN	denotes	selection
T5897	19820-19824	IN	denotes	from
T5898	19825-19826	DT	denotes	a
T5899	19827-19831	NN	denotes	pool
T5900	19832-19834	IN	denotes	of
T5901	19835-19841	JJ	denotes	double
T5903	19841-19842	HYPH	denotes	-
T5902	19842-19850	VBN	denotes	stranded
T5904	19851-19867	NNS	denotes	oligonucleotides
T5905	19868-19872	IN	denotes	with
T5906	19873-19879	JJ	denotes	random
T5907	19880-19889	NNS	denotes	sequences
T5888	19890-19897	VBD	denotes	yielded
T5908	19898-19900	DT	denotes	no
T5910	19901-19904	JJ	denotes	new
T5911	19905-19912	NN	denotes	binding
T5909	19913-19918	NN	denotes	motif
T5912	19919-19922	IN	denotes	for
T5913	19923-19926	NN	denotes	MTF
T5914	19926-19927	HYPH	denotes	-
T5915	19927-19928	CD	denotes	1
T5916	19929-19931	IN	denotes	in
T5917	19932-19940	NN	denotes	addition
T5918	19941-19943	IN	denotes	to
T5919	19944-19947	DT	denotes	the
T5921	19948-19953	JJ	denotes	known
T5920	19954-19958	NNS	denotes	MREs
T5922	19959-19960	-LRB-	denotes	(
T5923	19960-19962	CD	denotes	34
T5924	19962-19963	-RRB-	denotes	)
T5925	19963-19964	.	denotes	.
T5926	19964-20119	sentence	denotes	Thus, an MRE sequence is to date the only indication for a direct MTF-1 target gene, and four MRE-containing target gene candidates were further analyzed.
T5927	19965-19969	RB	denotes	Thus
T5929	19969-19971	,	denotes	,
T5930	19971-19973	DT	denotes	an
T5932	19974-19977	NN	denotes	MRE
T5931	19978-19986	NN	denotes	sequence
T5928	19987-19989	VBZ	denotes	is
T5933	19990-19992	IN	denotes	to
T5934	19993-19997	NN	denotes	date
T5935	19998-20001	DT	denotes	the
T5937	20002-20006	JJ	denotes	only
T5936	20007-20017	NN	denotes	indication
T5938	20018-20021	IN	denotes	for
T5939	20022-20023	DT	denotes	a
T5941	20024-20030	JJ	denotes	direct
T5942	20031-20034	NN	denotes	MTF
T5943	20034-20035	HYPH	denotes	-
T5944	20035-20036	CD	denotes	1
T5945	20037-20043	NN	denotes	target
T5940	20044-20048	NN	denotes	gene
T5946	20048-20050	,	denotes	,
T5947	20050-20053	CC	denotes	and
T5948	20054-20058	CD	denotes	four
T5950	20059-20062	NN	denotes	MRE
T5952	20062-20063	HYPH	denotes	-
T5951	20063-20073	VBG	denotes	containing
T5953	20074-20080	NN	denotes	target
T5954	20081-20085	NN	denotes	gene
T5949	20086-20096	NNS	denotes	candidates
T5956	20097-20101	VBD	denotes	were
T5957	20102-20109	RB	denotes	further
T5955	20110-20118	VBN	denotes	analyzed
T5958	20118-20119	.	denotes	.
T6000	20121-20126	JJ	denotes	Basal
T6001	20127-20137	NN	denotes	expression
T6003	20138-20140	IN	denotes	of
T6004	20141-20146	NN	denotes	Sepw1
T6002	20147-20154	VBZ	denotes	depends
T6005	20155-20157	IN	denotes	on
T6006	20158-20161	NN	denotes	MTF
T6007	20161-20162	HYPH	denotes	-
T6008	20162-20163	CD	denotes	1
T6009	20163-20313	sentence	denotes	Sepw1 was found in microarray analysis to be significantly downregulated in livers from cadmium- and mock-treated Mtf1Mx-cre mice (Table 1, a and c).
T6010	20164-20169	NN	denotes	Sepw1
T6012	20170-20173	VBD	denotes	was
T6011	20174-20179	VBN	denotes	found
T6013	20180-20182	IN	denotes	in
T6014	20183-20193	NN	denotes	microarray
T6015	20194-20202	NN	denotes	analysis
T6016	20203-20205	TO	denotes	to
T6017	20206-20208	VB	denotes	be
T6018	20209-20222	RB	denotes	significantly
T6019	20223-20236	JJ	denotes	downregulated
T6020	20237-20239	IN	denotes	in
T6021	20240-20246	NNS	denotes	livers
T6022	20247-20251	IN	denotes	from
T6023	20252-20259	NN	denotes	cadmium
T6025	20259-20260	HYPH	denotes	-
T6026	20261-20264	CC	denotes	and
T6027	20265-20269	JJ	denotes	mock
T6028	20269-20270	HYPH	denotes	-
T6024	20270-20277	VBN	denotes	treated
T6030	20278-20284	NN	denotes	Mtf1Mx
T6032	20284-20285	HYPH	denotes	-
T6031	20285-20288	NN	denotes	cre
T6029	20289-20293	NNS	denotes	mice
T6033	20294-20295	-LRB-	denotes	(
T6035	20295-20300	NN	denotes	Table
T6036	20301-20302	CD	denotes	1
T6037	20302-20304	,	denotes	,
T6034	20304-20305	NN	denotes	a
T6038	20306-20309	CC	denotes	and
T6039	20310-20311	NN	denotes	c
T6040	20311-20312	-RRB-	denotes	)
T6041	20312-20313	.	denotes	.
T6042	20313-20440	sentence	denotes	SEPW1 is a selenocysteine-containing protein that binds glutathione (35) and is thought to act as an antioxidant in vivo (36).
T6043	20314-20319	NN	denotes	SEPW1
T6044	20320-20322	VBZ	denotes	is
T6045	20323-20324	DT	denotes	a
T6047	20325-20339	NN	denotes	selenocysteine
T6049	20339-20340	HYPH	denotes	-
T6048	20340-20350	VBG	denotes	containing
T6046	20351-20358	NN	denotes	protein
T6050	20359-20363	WDT	denotes	that
T6051	20364-20369	VBZ	denotes	binds
T6052	20370-20381	NN	denotes	glutathione
T6053	20382-20383	-LRB-	denotes	(
T6054	20383-20385	CD	denotes	35
T6055	20385-20386	-RRB-	denotes	)
T6056	20387-20390	CC	denotes	and
T6057	20391-20393	VBZ	denotes	is
T6058	20394-20401	VBN	denotes	thought
T6059	20402-20404	TO	denotes	to
T6060	20405-20408	VB	denotes	act
T6061	20409-20411	IN	denotes	as
T6062	20412-20414	DT	denotes	an
T6063	20415-20426	NN	denotes	antioxidant
T6064	20427-20429	FW	denotes	in
T6065	20430-20434	FW	denotes	vivo
T6066	20435-20436	-LRB-	denotes	(
T6067	20436-20438	CD	denotes	36
T6068	20438-20439	-RRB-	denotes	)
T6069	20439-20440	.	denotes	.
T6070	20440-20623	sentence	denotes	Sepw1 expression in livers of pI–pC-induced, mock- or cadmium-treated Mtf1Mx-cre and Mtf1loxP mice was further analyzed by semiquantitative RT–PCRs and S1 analysis (Figure 2a and b).
T6071	20441-20446	NN	denotes	Sepw1
T6072	20447-20457	NN	denotes	expression
T6074	20458-20460	IN	denotes	in
T6075	20461-20467	NNS	denotes	livers
T6076	20468-20470	IN	denotes	of
T6077	20471-20473	NN	denotes	pI
T6079	20473-20474	HYPH	denotes	–
T6078	20474-20476	NN	denotes	pC
T6081	20476-20477	HYPH	denotes	-
T6080	20477-20484	VBN	denotes	induced
T6083	20484-20486	,	denotes	,
T6084	20486-20490	JJ	denotes	mock
T6086	20490-20491	HYPH	denotes	-
T6087	20492-20494	CC	denotes	or
T6088	20495-20502	NN	denotes	cadmium
T6089	20502-20503	HYPH	denotes	-
T6085	20503-20510	VBN	denotes	treated
T6090	20511-20517	NN	denotes	Mtf1Mx
T6092	20517-20518	HYPH	denotes	-
T6091	20518-20521	NN	denotes	cre
T6093	20522-20525	CC	denotes	and
T6094	20526-20534	NN	denotes	Mtf1loxP
T6082	20535-20539	NNS	denotes	mice
T6095	20540-20543	VBD	denotes	was
T6096	20544-20551	RB	denotes	further
T6073	20552-20560	VBN	denotes	analyzed
T6097	20561-20563	IN	denotes	by
T6098	20564-20580	JJ	denotes	semiquantitative
T6100	20581-20583	NN	denotes	RT
T6102	20583-20584	HYPH	denotes	–
T6101	20584-20588	NNS	denotes	PCRs
T6103	20589-20592	CC	denotes	and
T6104	20593-20595	NN	denotes	S1
T6099	20596-20604	NN	denotes	analysis
T6105	20605-20606	-LRB-	denotes	(
T6107	20606-20612	NN	denotes	Figure
T6106	20613-20615	NN	denotes	2a
T6108	20616-20619	CC	denotes	and
T6109	20620-20621	NN	denotes	b
T6110	20621-20622	-RRB-	denotes	)
T6111	20622-20623	.	denotes	.
T6112	20623-20778	sentence	denotes	In accordance with microarray data a slight, if any, upregulation of Sepw1 transcription was observed in livers from Mtf1loxP mice upon cadmium treatment.
T6113	20624-20626	IN	denotes	In
T6115	20627-20637	NN	denotes	accordance
T6116	20638-20642	IN	denotes	with
T6117	20643-20653	NN	denotes	microarray
T6118	20654-20658	NNS	denotes	data
T6119	20659-20660	DT	denotes	a
T6121	20661-20667	JJ	denotes	slight
T6122	20667-20669	,	denotes	,
T6124	20669-20671	IN	denotes	if
T6123	20672-20675	DT	denotes	any
T6125	20675-20677	,	denotes	,
T6120	20677-20689	NN	denotes	upregulation
T6126	20690-20692	IN	denotes	of
T6127	20693-20698	NN	denotes	Sepw1
T6128	20699-20712	NN	denotes	transcription
T6129	20713-20716	VBD	denotes	was
T6114	20717-20725	VBN	denotes	observed
T6130	20726-20728	IN	denotes	in
T6131	20729-20735	NNS	denotes	livers
T6132	20736-20740	IN	denotes	from
T6133	20741-20749	NN	denotes	Mtf1loxP
T6134	20750-20754	NNS	denotes	mice
T6135	20755-20759	IN	denotes	upon
T6136	20760-20767	NN	denotes	cadmium
T6137	20768-20777	NN	denotes	treatment
T6138	20777-20778	.	denotes	.
T6139	20778-20934	sentence	denotes	The basal level was reduced in livers from mock- and cadmium-treated Mtf1Mx-cre mice, indicating that MTF-1 is important for the basal expression of Sepw1.
T6140	20779-20782	DT	denotes	The
T6142	20783-20788	JJ	denotes	basal
T6141	20789-20794	NN	denotes	level
T6144	20795-20798	VBD	denotes	was
T6143	20799-20806	VBN	denotes	reduced
T6145	20807-20809	IN	denotes	in
T6146	20810-20816	NNS	denotes	livers
T6147	20817-20821	IN	denotes	from
T6148	20822-20826	JJ	denotes	mock
T6150	20826-20827	HYPH	denotes	-
T6151	20828-20831	CC	denotes	and
T6152	20832-20839	NN	denotes	cadmium
T6153	20839-20840	HYPH	denotes	-
T6149	20840-20847	VBN	denotes	treated
T6155	20848-20854	NN	denotes	Mtf1Mx
T6157	20854-20855	HYPH	denotes	-
T6156	20855-20858	NN	denotes	cre
T6154	20859-20863	NNS	denotes	mice
T6158	20863-20865	,	denotes	,
T6159	20865-20875	VBG	denotes	indicating
T6160	20876-20880	IN	denotes	that
T6162	20881-20884	NN	denotes	MTF
T6163	20884-20885	HYPH	denotes	-
T6164	20885-20886	CD	denotes	1
T6161	20887-20889	VBZ	denotes	is
T6165	20890-20899	JJ	denotes	important
T6166	20900-20903	IN	denotes	for
T6167	20904-20907	DT	denotes	the
T6169	20908-20913	JJ	denotes	basal
T6168	20914-20924	NN	denotes	expression
T6170	20925-20927	IN	denotes	of
T6171	20928-20933	NN	denotes	Sepw1
T6172	20933-20934	.	denotes	.
T6173	20934-21055	sentence	denotes	Three MRE core consensus sequences were found in the region upstream of the mouse Sepw1 transcription start (Figure 2c).
T6174	20935-20940	CD	denotes	Three
T6176	20941-20944	NN	denotes	MRE
T6177	20945-20949	NN	denotes	core
T6178	20950-20959	NN	denotes	consensus
T6175	20960-20969	NNS	denotes	sequences
T6180	20970-20974	VBD	denotes	were
T6179	20975-20980	VBN	denotes	found
T6181	20981-20983	IN	denotes	in
T6182	20984-20987	DT	denotes	the
T6183	20988-20994	NN	denotes	region
T6184	20995-21003	RB	denotes	upstream
T6185	21004-21006	IN	denotes	of
T6186	21007-21010	DT	denotes	the
T6188	21011-21016	NN	denotes	mouse
T6189	21017-21022	NN	denotes	Sepw1
T6190	21023-21036	NN	denotes	transcription
T6187	21037-21042	NN	denotes	start
T6191	21043-21044	-LRB-	denotes	(
T6193	21044-21050	NN	denotes	Figure
T6192	21051-21053	NN	denotes	2c
T6194	21053-21054	-RRB-	denotes	)
T6195	21054-21055	.	denotes	.
T6196	21055-21228	sentence	denotes	Two of them in opposite orientation overlap almost completely proximal to the transcription start (MRE1, −40 bp), the third one is located further upstream (MRE2, −527 bp).
T6197	21056-21059	CD	denotes	Two
T6199	21060-21062	IN	denotes	of
T6200	21063-21067	PRP	denotes	them
T6201	21068-21070	IN	denotes	in
T6202	21071-21079	JJ	denotes	opposite
T6203	21080-21091	NN	denotes	orientation
T6198	21092-21099	VBP	denotes	overlap
T6205	21100-21106	RB	denotes	almost
T6206	21107-21117	RB	denotes	completely
T6207	21118-21126	RB	denotes	proximal
T6208	21127-21129	IN	denotes	to
T6209	21130-21133	DT	denotes	the
T6211	21134-21147	NN	denotes	transcription
T6210	21148-21153	NN	denotes	start
T6212	21154-21155	-LRB-	denotes	(
T6214	21155-21159	NN	denotes	MRE1
T6215	21159-21161	,	denotes	,
T6216	21161-21162	SYM	denotes	−
T6217	21162-21164	CD	denotes	40
T6213	21165-21167	NN	denotes	bp
T6218	21167-21168	-RRB-	denotes	)
T6219	21168-21170	,	denotes	,
T6220	21170-21173	DT	denotes	the
T6222	21174-21179	JJ	denotes	third
T6221	21180-21183	NN	denotes	one
T6223	21184-21186	VBZ	denotes	is
T6204	21187-21194	VBN	denotes	located
T6224	21195-21202	RB	denotes	further
T6225	21203-21211	RB	denotes	upstream
T6226	21212-21213	-LRB-	denotes	(
T6228	21213-21217	NN	denotes	MRE2
T6229	21217-21219	,	denotes	,
T6230	21219-21220	SYM	denotes	−
T6231	21220-21223	CD	denotes	527
T6227	21224-21226	NN	denotes	bp
T6232	21226-21227	-RRB-	denotes	)
T6233	21227-21228	.	denotes	.
T6234	21228-21389	sentence	denotes	Specific binding of MTF-1 to Sepw1 MRE1 but not MRE2 oligonucleotide was observed in EMSA with liver protein extract from an Mtf1loxP control mouse (Figure 2d).
T6235	21229-21237	JJ	denotes	Specific
T6236	21238-21245	NN	denotes	binding
T6238	21246-21248	IN	denotes	of
T6239	21249-21252	NN	denotes	MTF
T6240	21252-21253	HYPH	denotes	-
T6241	21253-21254	CD	denotes	1
T6242	21255-21257	IN	denotes	to
T6243	21258-21263	NN	denotes	Sepw1
T6245	21264-21268	NN	denotes	MRE1
T6246	21269-21272	CC	denotes	but
T6247	21273-21276	RB	denotes	not
T6248	21277-21281	NN	denotes	MRE2
T6244	21282-21297	NN	denotes	oligonucleotide
T6249	21298-21301	VBD	denotes	was
T6237	21302-21310	VBN	denotes	observed
T6250	21311-21313	IN	denotes	in
T6251	21314-21318	NN	denotes	EMSA
T6252	21319-21323	IN	denotes	with
T6253	21324-21329	NN	denotes	liver
T6254	21330-21337	NN	denotes	protein
T6255	21338-21345	NN	denotes	extract
T6256	21346-21350	IN	denotes	from
T6257	21351-21353	DT	denotes	an
T6259	21354-21362	NN	denotes	Mtf1loxP
T6260	21363-21370	NN	denotes	control
T6258	21371-21376	NN	denotes	mouse
T6261	21377-21378	-LRB-	denotes	(
T6263	21378-21384	NN	denotes	Figure
T6262	21385-21387	NN	denotes	2d
T6264	21387-21388	-RRB-	denotes	)
T6265	21388-21389	.	denotes	.
T6266	21389-21564	sentence	denotes	As a control, no binding to MRE1 was detected with extract from a pI–pC-induced Mtf1Mx-cre mouse, confirming that the bandshift was indeed dependent on the presence of MTF-1.
T6267	21390-21392	IN	denotes	As
T6269	21393-21394	DT	denotes	a
T6270	21395-21402	NN	denotes	control
T6271	21402-21404	,	denotes	,
T6272	21404-21406	DT	denotes	no
T6273	21407-21414	NN	denotes	binding
T6274	21415-21417	IN	denotes	to
T6275	21418-21422	NN	denotes	MRE1
T6276	21423-21426	VBD	denotes	was
T6268	21427-21435	VBN	denotes	detected
T6277	21436-21440	IN	denotes	with
T6278	21441-21448	NN	denotes	extract
T6279	21449-21453	IN	denotes	from
T6280	21454-21455	DT	denotes	a
T6282	21456-21458	NN	denotes	pI
T6284	21458-21459	HYPH	denotes	–
T6283	21459-21461	NN	denotes	pC
T6286	21461-21462	HYPH	denotes	-
T6285	21462-21469	VBN	denotes	induced
T6287	21470-21476	NN	denotes	Mtf1Mx
T6289	21476-21477	HYPH	denotes	-
T6288	21477-21480	NN	denotes	cre
T6281	21481-21486	NN	denotes	mouse
T6290	21486-21488	,	denotes	,
T6291	21488-21498	VBG	denotes	confirming
T6292	21499-21503	IN	denotes	that
T6294	21504-21507	DT	denotes	the
T6295	21508-21517	NN	denotes	bandshift
T6293	21518-21521	VBD	denotes	was
T6296	21522-21528	RB	denotes	indeed
T6297	21529-21538	JJ	denotes	dependent
T6298	21539-21541	IN	denotes	on
T6299	21542-21545	DT	denotes	the
T6300	21546-21554	NN	denotes	presence
T6301	21555-21557	IN	denotes	of
T6302	21558-21561	NN	denotes	MTF
T6303	21561-21562	HYPH	denotes	-
T6304	21562-21563	CD	denotes	1
T6305	21563-21564	.	denotes	.
T6587	21566-21573	NN	denotes	Cadmium
T6588	21574-21582	NN	denotes	response
T6590	21583-21585	IN	denotes	of
T6591	21586-21591	NN	denotes	Ndrg1
T6589	21592-21599	VBZ	denotes	depends
T6592	21600-21602	IN	denotes	on
T6593	21603-21606	NN	denotes	MTF
T6594	21606-21607	HYPH	denotes	-
T6595	21607-21608	CD	denotes	1
T6596	21608-21785	sentence	denotes	Ndrg1 was significantly downregulated in microarrays of liver transcripts from cadmium-treated Mtf1Mx-cre mice compared to similarly treated Mtf1loxP control mice (Table 1, c).
T6597	21609-21614	NN	denotes	Ndrg1
T6599	21615-21618	VBD	denotes	was
T6600	21619-21632	RB	denotes	significantly
T6598	21633-21646	VBN	denotes	downregulated
T6601	21647-21649	IN	denotes	in
T6602	21650-21661	NNS	denotes	microarrays
T6603	21662-21664	IN	denotes	of
T6604	21665-21670	NN	denotes	liver
T6605	21671-21682	NNS	denotes	transcripts
T6606	21683-21687	IN	denotes	from
T6607	21688-21695	NN	denotes	cadmium
T6609	21695-21696	HYPH	denotes	-
T6608	21696-21703	VBN	denotes	treated
T6611	21704-21710	NN	denotes	Mtf1Mx
T6613	21710-21711	HYPH	denotes	-
T6612	21711-21714	NN	denotes	cre
T6610	21715-21719	NNS	denotes	mice
T6614	21720-21728	VBN	denotes	compared
T6615	21729-21731	IN	denotes	to
T6616	21732-21741	RB	denotes	similarly
T6617	21742-21749	VBN	denotes	treated
T6619	21750-21758	NN	denotes	Mtf1loxP
T6620	21759-21766	NN	denotes	control
T6618	21767-21771	NNS	denotes	mice
T6621	21772-21773	-LRB-	denotes	(
T6623	21773-21778	NN	denotes	Table
T6624	21779-21780	CD	denotes	1
T6625	21780-21782	,	denotes	,
T6622	21782-21783	NN	denotes	c
T6626	21783-21784	-RRB-	denotes	)
T6627	21784-21785	.	denotes	.
T6628	21785-21967	sentence	denotes	Ndrg1 probably has some role in stress response since various stimuli, including hypoxia and nickel compounds, activate expression of rodent Ndrg1 and/or its human ortholog (37–40).
T6629	21786-21791	NN	denotes	Ndrg1
T6631	21792-21800	RB	denotes	probably
T6630	21801-21804	VBZ	denotes	has
T6632	21805-21809	DT	denotes	some
T6633	21810-21814	NN	denotes	role
T6634	21815-21817	IN	denotes	in
T6635	21818-21824	NN	denotes	stress
T6636	21825-21833	NN	denotes	response
T6637	21834-21839	IN	denotes	since
T6639	21840-21847	JJ	denotes	various
T6640	21848-21855	NNS	denotes	stimuli
T6641	21855-21857	,	denotes	,
T6642	21857-21866	VBG	denotes	including
T6643	21867-21874	NN	denotes	hypoxia
T6645	21875-21878	CC	denotes	and
T6646	21879-21885	NN	denotes	nickel
T6644	21886-21895	NNS	denotes	compounds
T6647	21895-21897	,	denotes	,
T6638	21897-21905	VBP	denotes	activate
T6648	21906-21916	NN	denotes	expression
T6649	21917-21919	IN	denotes	of
T6650	21920-21926	NN	denotes	rodent
T6651	21927-21932	NN	denotes	Ndrg1
T6652	21933-21936	CC	denotes	and
T6653	21936-21937	HYPH	denotes	/
T6654	21937-21939	CC	denotes	or
T6655	21940-21943	PRP$	denotes	its
T6657	21944-21949	JJ	denotes	human
T6656	21950-21958	NN	denotes	ortholog
T6658	21959-21960	-LRB-	denotes	(
T6659	21960-21962	CD	denotes	37
T6660	21962-21963	SYM	denotes	–
T6661	21963-21965	CD	denotes	40
T6662	21965-21966	-RRB-	denotes	)
T6663	21966-21967	.	denotes	.
T6664	21967-22279	sentence	denotes	The Ndrg1 microarray results were confirmed with semiquantitative RT–PCRs (Figure 3a): for Mtf1loxP control livers, a clear increase of Ndrg1 expression was observed after cadmium exposure; in livers from Mtf1Mx-cre mice, this cadmium response was not detectable, while basal expression was similar to controls.
T6665	21968-21971	DT	denotes	The
T6667	21972-21977	NN	denotes	Ndrg1
T6668	21978-21988	NN	denotes	microarray
T6666	21989-21996	NNS	denotes	results
T6670	21997-22001	VBD	denotes	were
T6669	22002-22011	VBN	denotes	confirmed
T6672	22012-22016	IN	denotes	with
T6673	22017-22033	JJ	denotes	semiquantitative
T6675	22034-22036	NN	denotes	RT
T6676	22036-22037	HYPH	denotes	–
T6674	22037-22041	NNS	denotes	PCRs
T6677	22042-22043	-LRB-	denotes	(
T6679	22043-22049	NN	denotes	Figure
T6678	22050-22052	NN	denotes	3a
T6680	22052-22053	-RRB-	denotes	)
T6681	22053-22055	:	denotes	:
T6682	22055-22058	IN	denotes	for
T6684	22059-22067	NN	denotes	Mtf1loxP
T6686	22068-22075	NN	denotes	control
T6685	22076-22082	NNS	denotes	livers
T6687	22082-22084	,	denotes	,
T6688	22084-22085	DT	denotes	a
T6690	22086-22091	JJ	denotes	clear
T6689	22092-22100	NN	denotes	increase
T6691	22101-22103	IN	denotes	of
T6692	22104-22109	NN	denotes	Ndrg1
T6693	22110-22120	NN	denotes	expression
T6694	22121-22124	VBD	denotes	was
T6683	22125-22133	VBN	denotes	observed
T6695	22134-22139	IN	denotes	after
T6696	22140-22147	NN	denotes	cadmium
T6697	22148-22156	NN	denotes	exposure
T6698	22156-22157	:	denotes	;
T6699	22158-22160	IN	denotes	in
T6700	22161-22167	NNS	denotes	livers
T6701	22168-22172	IN	denotes	from
T6702	22173-22179	NN	denotes	Mtf1Mx
T6704	22179-22180	HYPH	denotes	-
T6703	22180-22183	NN	denotes	cre
T6705	22184-22188	NNS	denotes	mice
T6706	22188-22190	,	denotes	,
T6707	22190-22194	DT	denotes	this
T6709	22195-22202	NN	denotes	cadmium
T6708	22203-22211	NN	denotes	response
T6671	22212-22215	VBD	denotes	was
T6710	22216-22219	RB	denotes	not
T6711	22220-22230	JJ	denotes	detectable
T6712	22230-22232	,	denotes	,
T6713	22232-22237	IN	denotes	while
T6715	22238-22243	JJ	denotes	basal
T6716	22244-22254	NN	denotes	expression
T6714	22255-22258	VBD	denotes	was
T6717	22259-22266	JJ	denotes	similar
T6718	22267-22269	IN	denotes	to
T6719	22270-22278	NNS	denotes	controls
T6720	22278-22279	.	denotes	.
T6721	22279-22353	sentence	denotes	This indicates that cadmium-induced expression of Ndrg1 depends on MTF-1.
T6722	22280-22284	DT	denotes	This
T6723	22285-22294	VBZ	denotes	indicates
T6724	22295-22299	IN	denotes	that
T6726	22300-22307	NN	denotes	cadmium
T6728	22307-22308	HYPH	denotes	-
T6727	22308-22315	VBN	denotes	induced
T6729	22316-22326	NN	denotes	expression
T6730	22327-22329	IN	denotes	of
T6731	22330-22335	NN	denotes	Ndrg1
T6725	22336-22343	VBZ	denotes	depends
T6732	22344-22346	IN	denotes	on
T6733	22347-22350	NN	denotes	MTF
T6734	22350-22351	HYPH	denotes	-
T6735	22351-22352	CD	denotes	1
T6736	22352-22353	.	denotes	.
T6737	22353-22460	sentence	denotes	Five MRE core consensus sequences are located upstream of the mouse Ndrg1 transcription start (Figure 3b).
T6738	22354-22358	CD	denotes	Five
T6740	22359-22362	NN	denotes	MRE
T6741	22363-22367	NN	denotes	core
T6742	22368-22377	NN	denotes	consensus
T6739	22378-22387	NNS	denotes	sequences
T6744	22388-22391	VBP	denotes	are
T6743	22392-22399	VBN	denotes	located
T6745	22400-22408	RB	denotes	upstream
T6746	22409-22411	IN	denotes	of
T6747	22412-22415	DT	denotes	the
T6749	22416-22421	NN	denotes	mouse
T6750	22422-22427	NN	denotes	Ndrg1
T6751	22428-22441	NN	denotes	transcription
T6748	22442-22447	NN	denotes	start
T6752	22448-22449	-LRB-	denotes	(
T6754	22449-22455	NN	denotes	Figure
T6753	22456-22458	NN	denotes	3b
T6755	22458-22459	-RRB-	denotes	)
T6756	22459-22460	.	denotes	.
T6757	22460-22579	sentence	denotes	Four of them are clustered (MRE1 to MRE4, −138 to −332 bp), the fifth one is located farther upstream (MRE5, −883 bp).
T6758	22461-22465	CD	denotes	Four
T6760	22466-22468	IN	denotes	of
T6761	22469-22473	PRP	denotes	them
T6762	22474-22477	VBP	denotes	are
T6759	22478-22487	VBN	denotes	clustered
T6764	22488-22489	-LRB-	denotes	(
T6766	22489-22493	NN	denotes	MRE1
T6767	22494-22496	IN	denotes	to
T6768	22497-22501	NN	denotes	MRE4
T6769	22501-22503	,	denotes	,
T6770	22503-22504	SYM	denotes	−
T6772	22504-22507	CD	denotes	138
T6773	22508-22510	IN	denotes	to
T6774	22511-22512	SYM	denotes	−
T6771	22512-22515	CD	denotes	332
T6765	22516-22518	NN	denotes	bp
T6775	22518-22519	-RRB-	denotes	)
T6776	22519-22521	,	denotes	,
T6777	22521-22524	DT	denotes	the
T6779	22525-22530	JJ	denotes	fifth
T6778	22531-22534	CD	denotes	one
T6780	22535-22537	VBZ	denotes	is
T6763	22538-22545	VBN	denotes	located
T6781	22546-22553	RB	denotes	farther
T6782	22554-22562	RB	denotes	upstream
T6783	22563-22564	-LRB-	denotes	(
T6785	22564-22568	NN	denotes	MRE5
T6786	22568-22570	,	denotes	,
T6787	22570-22571	SYM	denotes	−
T6788	22571-22574	CD	denotes	883
T6784	22575-22577	NN	denotes	bp
T6789	22577-22578	-RRB-	denotes	)
T6790	22578-22579	.	denotes	.
T6791	22579-22700	sentence	denotes	EMSA was performed to test whether MTF-1 is interacting with some or all of the four proximal MRE sequences (Figure 3c).
T6792	22580-22584	NN	denotes	EMSA
T6794	22585-22588	VBD	denotes	was
T6793	22589-22598	VBN	denotes	performed
T6795	22599-22601	TO	denotes	to
T6796	22602-22606	VB	denotes	test
T6797	22607-22614	IN	denotes	whether
T6799	22615-22618	NN	denotes	MTF
T6800	22618-22619	HYPH	denotes	-
T6801	22619-22620	CD	denotes	1
T6802	22621-22623	VBZ	denotes	is
T6798	22624-22635	VBG	denotes	interacting
T6803	22636-22640	IN	denotes	with
T6804	22641-22645	DT	denotes	some
T6805	22646-22648	CC	denotes	or
T6806	22649-22652	DT	denotes	all
T6807	22653-22655	IN	denotes	of
T6808	22656-22659	DT	denotes	the
T6810	22660-22664	CD	denotes	four
T6811	22665-22673	JJ	denotes	proximal
T6812	22674-22677	NN	denotes	MRE
T6809	22678-22687	NNS	denotes	sequences
T6813	22688-22689	-LRB-	denotes	(
T6815	22689-22695	NN	denotes	Figure
T6814	22696-22698	NN	denotes	3c
T6816	22698-22699	-RRB-	denotes	)
T6817	22699-22700	.	denotes	.
T6818	22700-22846	sentence	denotes	Separate oligonucleotides were tested for MRE1 and MRE2, whereas one oligonucleotide spanning both sequences was used for MRE3 and MRE4 (MRE3,4).
T6819	22701-22709	JJ	denotes	Separate
T6820	22710-22726	NNS	denotes	oligonucleotides
T6822	22727-22731	VBD	denotes	were
T6821	22732-22738	VBN	denotes	tested
T6823	22739-22742	IN	denotes	for
T6824	22743-22747	NN	denotes	MRE1
T6825	22748-22751	CC	denotes	and
T6826	22752-22756	NN	denotes	MRE2
T6827	22756-22758	,	denotes	,
T6828	22758-22765	IN	denotes	whereas
T6830	22766-22769	CD	denotes	one
T6831	22770-22785	NN	denotes	oligonucleotide
T6832	22786-22794	VBG	denotes	spanning
T6833	22795-22799	DT	denotes	both
T6834	22800-22809	NNS	denotes	sequences
T6835	22810-22813	VBD	denotes	was
T6829	22814-22818	VBN	denotes	used
T6836	22819-22822	IN	denotes	for
T6837	22823-22827	NN	denotes	MRE3
T6838	22828-22831	CC	denotes	and
T6839	22832-22836	NN	denotes	MRE4
T6840	22837-22838	-LRB-	denotes	(
T6842	22838-22842	NN	denotes	MRE3
T6843	22842-22843	,	denotes	,
T6841	22843-22844	CD	denotes	4
T6844	22844-22845	-RRB-	denotes	)
T6845	22845-22846	.	denotes	.
T6846	22846-23017	sentence	denotes	No complex was seen with MRE1, but specific MTF-1 complexes were observed for both the MRE2 and MRE3,4 oligonucleotides with liver protein extract from an Mtf1loxP mouse.
T6847	22847-22849	DT	denotes	No
T6848	22850-22857	NN	denotes	complex
T6850	22858-22861	VBD	denotes	was
T6849	22862-22866	VBN	denotes	seen
T6851	22867-22871	IN	denotes	with
T6852	22872-22876	NN	denotes	MRE1
T6853	22876-22878	,	denotes	,
T6854	22878-22881	CC	denotes	but
T6855	22882-22890	JJ	denotes	specific
T6857	22891-22894	NN	denotes	MTF
T6858	22894-22895	HYPH	denotes	-
T6859	22895-22896	CD	denotes	1
T6856	22897-22906	NNS	denotes	complexes
T6861	22907-22911	VBD	denotes	were
T6860	22912-22920	VBN	denotes	observed
T6862	22921-22924	IN	denotes	for
T6863	22925-22929	CC	denotes	both
T6865	22930-22933	DT	denotes	the
T6864	22934-22938	NN	denotes	MRE2
T6866	22939-22942	CC	denotes	and
T6867	22943-22947	NN	denotes	MRE3
T6868	22947-22948	,	denotes	,
T6869	22948-22949	CD	denotes	4
T6870	22950-22966	NNS	denotes	oligonucleotides
T6871	22967-22971	IN	denotes	with
T6872	22972-22977	NN	denotes	liver
T6874	22978-22985	NN	denotes	protein
T6873	22986-22993	NN	denotes	extract
T6875	22994-22998	IN	denotes	from
T6876	22999-23001	DT	denotes	an
T6878	23002-23010	NN	denotes	Mtf1loxP
T6877	23011-23016	NN	denotes	mouse
T6879	23016-23017	.	denotes	.
T6880	23017-23118	sentence	denotes	As expected, no bandshift was observed with protein extract from a mouse lacking MTF-1 (Mtf1Mx-cre).
T6881	23018-23020	IN	denotes	As
T6882	23021-23029	VBN	denotes	expected
T6884	23029-23031	,	denotes	,
T6885	23031-23033	DT	denotes	no
T6886	23034-23043	NN	denotes	bandshift
T6887	23044-23047	VBD	denotes	was
T6883	23048-23056	VBN	denotes	observed
T6888	23057-23061	IN	denotes	with
T6889	23062-23069	NN	denotes	protein
T6890	23070-23077	NN	denotes	extract
T6891	23078-23082	IN	denotes	from
T6892	23083-23084	DT	denotes	a
T6893	23085-23090	NN	denotes	mouse
T6894	23091-23098	VBG	denotes	lacking
T6895	23099-23102	NN	denotes	MTF
T6896	23102-23103	HYPH	denotes	-
T6897	23103-23104	CD	denotes	1
T6898	23105-23106	-LRB-	denotes	(
T6899	23106-23112	NN	denotes	Mtf1Mx
T6901	23112-23113	HYPH	denotes	-
T6900	23113-23116	NN	denotes	cre
T6902	23116-23117	-RRB-	denotes	)
T6903	23117-23118	.	denotes	.
T6936	23120-23127	NN	denotes	Cadmium
T6937	23128-23136	NN	denotes	response
T6939	23137-23139	IN	denotes	of
T6940	23140-23145	NN	denotes	Csrp1
T6938	23146-23153	VBZ	denotes	depends
T6941	23154-23156	IN	denotes	on
T6942	23157-23160	NN	denotes	MTF
T6943	23160-23161	HYPH	denotes	-
T6944	23161-23162	CD	denotes	1
T6945	23162-23310	sentence	denotes	Csrp1 was found in microarray analyses to be significantly downregulated in cadmium-treated Mtf1Mx-cre mice compared to Mtf1loxP mice (Table 1, c).
T6946	23163-23168	NN	denotes	Csrp1
T6948	23169-23172	VBD	denotes	was
T6947	23173-23178	VBN	denotes	found
T6949	23179-23181	IN	denotes	in
T6950	23182-23192	NN	denotes	microarray
T6951	23193-23201	NNS	denotes	analyses
T6952	23202-23204	TO	denotes	to
T6954	23205-23207	VB	denotes	be
T6955	23208-23221	RB	denotes	significantly
T6953	23222-23235	VBN	denotes	downregulated
T6956	23236-23238	IN	denotes	in
T6957	23239-23246	NN	denotes	cadmium
T6959	23246-23247	HYPH	denotes	-
T6958	23247-23254	VBN	denotes	treated
T6961	23255-23261	NN	denotes	Mtf1Mx
T6934	23261-23262	HYPH	denotes	-
T6962	23262-23265	NN	denotes	cre
T6960	23266-23270	NNS	denotes	mice
T6963	23271-23279	VBN	denotes	compared
T6964	23280-23282	IN	denotes	to
T6965	23283-23291	NN	denotes	Mtf1loxP
T6966	23292-23296	NNS	denotes	mice
T6967	23297-23298	-LRB-	denotes	(
T6969	23298-23303	NN	denotes	Table
T6970	23304-23305	CD	denotes	1
T6971	23305-23307	,	denotes	,
T6968	23307-23308	NN	denotes	c
T6972	23308-23309	-RRB-	denotes	)
T6973	23309-23310	.	denotes	.
T6974	23310-23458	sentence	denotes	CSRP1 is a member of the evolutionary conserved CRP family of proteins that have been implicated in myogenesis and cytoskeletal remodeling (41,42).
T6975	23311-23316	NN	denotes	CSRP1
T6976	23317-23319	VBZ	denotes	is
T6977	23320-23321	DT	denotes	a
T6978	23322-23328	NN	denotes	member
T6979	23329-23331	IN	denotes	of
T6980	23332-23335	DT	denotes	the
T6982	23336-23348	JJ	denotes	evolutionary
T6983	23349-23358	VBN	denotes	conserved
T6984	23359-23362	NN	denotes	CRP
T6981	23363-23369	NN	denotes	family
T6985	23370-23372	IN	denotes	of
T6935	23373-23381	NN	denotes	proteins
T6986	23382-23386	WDT	denotes	that
T6988	23387-23391	VBP	denotes	have
T6989	23392-23396	VBN	denotes	been
T6987	23397-23407	VBN	denotes	implicated
T6990	23408-23410	IN	denotes	in
T6991	23411-23421	NN	denotes	myogenesis
T6992	23422-23425	CC	denotes	and
T6993	23426-23438	JJ	denotes	cytoskeletal
T6994	23439-23449	NN	denotes	remodeling
T6995	23450-23451	-LRB-	denotes	(
T6997	23451-23453	CD	denotes	41
T6998	23453-23454	,	denotes	,
T6996	23454-23456	CD	denotes	42
T6999	23456-23457	-RRB-	denotes	)
T7000	23457-23458	.	denotes	.
T7001	23458-23613	sentence	denotes	Semiquantitative RT–PCRs confirmed the microarray results, namely, that Csrp1 expression is elevated in Mtf1loxP livers upon cadmium exposure (Figure 4a).
T7002	23459-23475	JJ	denotes	Semiquantitative
T7004	23476-23478	NN	denotes	RT
T7005	23478-23479	HYPH	denotes	–
T7003	23479-23483	NNS	denotes	PCRs
T7006	23484-23493	VBD	denotes	confirmed
T7007	23494-23497	DT	denotes	the
T7008	23498-23508	NN	denotes	microarray
T7009	23509-23516	VBZ	denotes	results
T7010	23516-23518	,	denotes	,
T7011	23518-23524	RB	denotes	namely
T7013	23524-23526	,	denotes	,
T7014	23526-23530	IN	denotes	that
T7015	23531-23536	NN	denotes	Csrp1
T7016	23537-23547	NN	denotes	expression
T7017	23548-23550	VBZ	denotes	is
T7012	23551-23559	VBN	denotes	elevated
T7018	23560-23562	IN	denotes	in
T7019	23563-23571	NN	denotes	Mtf1loxP
T7020	23572-23578	NNS	denotes	livers
T7021	23579-23583	IN	denotes	upon
T7022	23584-23591	NN	denotes	cadmium
T7023	23592-23600	NN	denotes	exposure
T7024	23601-23602	-LRB-	denotes	(
T7026	23602-23608	NN	denotes	Figure
T7025	23609-23611	NN	denotes	4a
T7027	23611-23612	-RRB-	denotes	)
T7028	23612-23613	.	denotes	.
T7029	23613-23759	sentence	denotes	In contrast, no cadmium response was detectable in livers from Mtf1Mx-cre mice, suggesting that MTF-1 is required for cadmium induction of Csrp1.
T7030	23614-23616	IN	denotes	In
T7032	23617-23625	NN	denotes	contrast
T7033	23625-23627	,	denotes	,
T7034	23627-23629	DT	denotes	no
T7036	23630-23637	NN	denotes	cadmium
T7035	23638-23646	NN	denotes	response
T7031	23647-23650	VBD	denotes	was
T7037	23651-23661	JJ	denotes	detectable
T7038	23662-23664	IN	denotes	in
T7039	23665-23671	NNS	denotes	livers
T7040	23672-23676	IN	denotes	from
T7041	23677-23683	NN	denotes	Mtf1Mx
T7043	23683-23684	HYPH	denotes	-
T7042	23684-23687	NN	denotes	cre
T7044	23688-23692	NNS	denotes	mice
T7045	23692-23694	,	denotes	,
T7046	23694-23704	VBG	denotes	suggesting
T7047	23705-23709	IN	denotes	that
T7049	23710-23713	NN	denotes	MTF
T7050	23713-23714	HYPH	denotes	-
T7051	23714-23715	CD	denotes	1
T7052	23716-23718	VBZ	denotes	is
T7048	23719-23727	VBN	denotes	required
T7053	23728-23731	IN	denotes	for
T7054	23732-23739	NN	denotes	cadmium
T7055	23740-23749	NN	denotes	induction
T7056	23750-23752	IN	denotes	of
T7057	23753-23758	NN	denotes	Csrp1
T7058	23758-23759	.	denotes	.
T7059	23759-23942	sentence	denotes	Three MRE core consensus sequences were found upstream of the Csrp1 transcription start (MRE2 to MRE4, −56 to −366 bp), one was found immediately downstream (MRE1, +7 bp; Figure 4b).
T7060	23760-23765	CD	denotes	Three
T7062	23766-23769	NN	denotes	MRE
T7063	23770-23774	NN	denotes	core
T7064	23775-23784	NN	denotes	consensus
T7061	23785-23794	NNS	denotes	sequences
T7066	23795-23799	VBD	denotes	were
T7065	23800-23805	VBN	denotes	found
T7068	23806-23814	RB	denotes	upstream
T7069	23815-23817	IN	denotes	of
T7070	23818-23821	DT	denotes	the
T7072	23822-23827	NN	denotes	Csrp1
T7073	23828-23841	NN	denotes	transcription
T7071	23842-23847	NN	denotes	start
T7074	23848-23849	-LRB-	denotes	(
T7076	23849-23853	NN	denotes	MRE2
T7077	23854-23856	IN	denotes	to
T7078	23857-23861	NN	denotes	MRE4
T7079	23861-23863	,	denotes	,
T7080	23863-23864	SYM	denotes	−
T7081	23864-23866	CD	denotes	56
T7083	23867-23869	IN	denotes	to
T7084	23870-23871	SYM	denotes	−
T7082	23871-23874	CD	denotes	366
T7075	23875-23877	NN	denotes	bp
T7085	23877-23878	-RRB-	denotes	)
T7086	23878-23880	,	denotes	,
T7087	23880-23883	CD	denotes	one
T7088	23884-23887	VBD	denotes	was
T7067	23888-23893	VBN	denotes	found
T7089	23894-23905	RB	denotes	immediately
T7090	23906-23916	RB	denotes	downstream
T7091	23917-23918	-LRB-	denotes	(
T7093	23918-23922	NN	denotes	MRE1
T7094	23922-23924	,	denotes	,
T7095	23924-23925	SYM	denotes	+
T7096	23925-23926	CD	denotes	7
T7097	23927-23929	NN	denotes	bp
T7098	23929-23930	:	denotes	;
T7099	23931-23937	NN	denotes	Figure
T7092	23938-23940	NN	denotes	4b
T7100	23940-23941	-RRB-	denotes	)
T7101	23941-23942	.	denotes	.
T7102	23942-24177	sentence	denotes	Specific binding of MTF-1 was observed with EMSA for MRE2 oligonucleotide and protein extract from an Mtf1loxP liver, but not an Mtf1Mx-cre liver extract lacking MTF-1, confirming the participation of MTF-1 in the complex (Figure 4c).
T7103	23943-23951	JJ	denotes	Specific
T7104	23952-23959	NN	denotes	binding
T7106	23960-23962	IN	denotes	of
T7107	23963-23966	NN	denotes	MTF
T7108	23966-23967	HYPH	denotes	-
T7109	23967-23968	CD	denotes	1
T7110	23969-23972	VBD	denotes	was
T7105	23973-23981	VBN	denotes	observed
T7111	23982-23986	IN	denotes	with
T7112	23987-23991	NN	denotes	EMSA
T7113	23992-23995	IN	denotes	for
T7114	23996-24000	NN	denotes	MRE2
T7115	24001-24016	NN	denotes	oligonucleotide
T7116	24017-24020	CC	denotes	and
T7117	24021-24028	NN	denotes	protein
T7118	24029-24036	NN	denotes	extract
T7119	24037-24041	IN	denotes	from
T7120	24042-24044	DT	denotes	an
T7122	24045-24053	NN	denotes	Mtf1loxP
T7121	24054-24059	NN	denotes	liver
T7124	24059-24061	,	denotes	,
T7125	24061-24064	CC	denotes	but
T7126	24065-24068	RB	denotes	not
T7127	24069-24071	DT	denotes	an
T7129	24072-24078	NN	denotes	Mtf1Mx
T7131	24078-24079	HYPH	denotes	-
T7130	24079-24082	NN	denotes	cre
T7128	24083-24088	NN	denotes	liver
T7123	24089-24096	NN	denotes	extract
T7132	24097-24104	VBG	denotes	lacking
T7133	24105-24108	NN	denotes	MTF
T7134	24108-24109	HYPH	denotes	-
T7135	24109-24110	CD	denotes	1
T7136	24110-24112	,	denotes	,
T7137	24112-24122	VBG	denotes	confirming
T7138	24123-24126	DT	denotes	the
T7139	24127-24140	NN	denotes	participation
T7140	24141-24143	IN	denotes	of
T7141	24144-24147	NN	denotes	MTF
T7142	24147-24148	HYPH	denotes	-
T7143	24148-24149	CD	denotes	1
T7144	24150-24152	IN	denotes	in
T7145	24153-24156	DT	denotes	the
T7146	24157-24164	NN	denotes	complex
T7147	24165-24166	-LRB-	denotes	(
T7149	24166-24172	NN	denotes	Figure
T7148	24173-24175	NN	denotes	4c
T7150	24175-24176	-RRB-	denotes	)
T7151	24176-24177	.	denotes	.
T7421	24179-24182	NN	denotes	MTF
T7423	24182-24183	HYPH	denotes	-
T7424	24183-24184	CD	denotes	1
T7422	24185-24193	VBZ	denotes	inhibits
T7425	24194-24204	NN	denotes	expression
T7426	24205-24207	IN	denotes	of
T7427	24208-24216	NN	denotes	Slc39a10
T7428	24216-24403	sentence	denotes	Slc39a10 was detected in microarray analysis to be significantly upregulated in livers from both mock- and cadmium-treated Mtf1Mx-cre mice compared to control animals (Table 1, b and d).
T7429	24217-24225	NN	denotes	Slc39a10
T7431	24226-24229	VBD	denotes	was
T7430	24230-24238	VBN	denotes	detected
T7432	24239-24241	IN	denotes	in
T7433	24242-24252	NN	denotes	microarray
T7434	24253-24261	NN	denotes	analysis
T7435	24262-24264	TO	denotes	to
T7437	24265-24267	VB	denotes	be
T7438	24268-24281	RB	denotes	significantly
T7436	24282-24293	VBN	denotes	upregulated
T7439	24294-24296	IN	denotes	in
T7440	24297-24303	NNS	denotes	livers
T7441	24304-24308	IN	denotes	from
T7442	24309-24313	CC	denotes	both
T7443	24314-24318	JJ	denotes	mock
T7445	24318-24319	HYPH	denotes	-
T7446	24320-24323	CC	denotes	and
T7447	24324-24331	NN	denotes	cadmium
T7448	24331-24332	HYPH	denotes	-
T7444	24332-24339	VBN	denotes	treated
T7450	24340-24346	NN	denotes	Mtf1Mx
T7452	24346-24347	HYPH	denotes	-
T7451	24347-24350	NN	denotes	cre
T7449	24351-24355	NNS	denotes	mice
T7453	24356-24364	VBN	denotes	compared
T7454	24365-24367	IN	denotes	to
T7455	24368-24375	NN	denotes	control
T7456	24376-24383	NNS	denotes	animals
T7457	24384-24385	-LRB-	denotes	(
T7459	24385-24390	NN	denotes	Table
T7460	24391-24392	CD	denotes	1
T7461	24392-24394	,	denotes	,
T7458	24394-24395	NN	denotes	b
T7462	24396-24399	CC	denotes	and
T7463	24400-24401	NN	denotes	d
T7464	24401-24402	-RRB-	denotes	)
T7465	24402-24403	.	denotes	.
T7466	24403-24618	sentence	denotes	SLC39 proteins are members of the Zrt- and Irt-like protein (ZIP) family of metal ion transporters that transport, with no known exception, metal ion substrates across cellular membranes into the cytoplasm (43,44).
T7467	24404-24409	NN	denotes	SLC39
T7468	24410-24418	NN	denotes	proteins
T7469	24419-24422	VBP	denotes	are
T7470	24423-24430	NNS	denotes	members
T7471	24431-24433	IN	denotes	of
T7472	24434-24437	DT	denotes	the
T7474	24438-24441	NN	denotes	Zrt
T7476	24441-24442	HYPH	denotes	-
T7477	24443-24446	CC	denotes	and
T7478	24447-24450	NN	denotes	Irt
T7479	24450-24451	HYPH	denotes	-
T7475	24451-24455	JJ	denotes	like
T7480	24456-24463	NN	denotes	protein
T7481	24464-24465	-LRB-	denotes	(
T7482	24465-24468	NN	denotes	ZIP
T7483	24468-24469	-RRB-	denotes	)
T7473	24470-24476	NN	denotes	family
T7484	24477-24479	IN	denotes	of
T7485	24480-24485	NN	denotes	metal
T7487	24486-24489	NN	denotes	ion
T7486	24490-24502	NNS	denotes	transporters
T7488	24503-24507	WDT	denotes	that
T7489	24508-24517	VBP	denotes	transport
T7490	24517-24519	,	denotes	,
T7491	24519-24523	IN	denotes	with
T7492	24524-24526	DT	denotes	no
T7494	24527-24532	JJ	denotes	known
T7493	24533-24542	NN	denotes	exception
T7495	24542-24544	,	denotes	,
T7496	24544-24549	NN	denotes	metal
T7497	24550-24553	NN	denotes	ion
T7498	24554-24564	NNS	denotes	substrates
T7499	24565-24571	IN	denotes	across
T7500	24572-24580	JJ	denotes	cellular
T7501	24581-24590	NNS	denotes	membranes
T7502	24591-24595	IN	denotes	into
T7503	24596-24599	DT	denotes	the
T7504	24600-24609	NN	denotes	cytoplasm
T7505	24610-24611	-LRB-	denotes	(
T7507	24611-24613	CD	denotes	43
T7508	24613-24614	,	denotes	,
T7506	24614-24616	CD	denotes	44
T7509	24616-24617	-RRB-	denotes	)
T7510	24617-24618	.	denotes	.
T7511	24618-24776	sentence	denotes	In accordance with microarray data, semiquantitative RT–PCRs showed a downregulation of Slc39a10 expression in livers of Mtf1loxP mice upon cadmium exposure.
T7512	24619-24621	IN	denotes	In
T7514	24622-24632	NN	denotes	accordance
T7515	24633-24637	IN	denotes	with
T7516	24638-24648	NN	denotes	microarray
T7517	24649-24653	NNS	denotes	data
T7518	24653-24655	,	denotes	,
T7519	24655-24671	JJ	denotes	semiquantitative
T7521	24672-24674	NN	denotes	RT
T7522	24674-24675	HYPH	denotes	–
T7520	24675-24679	NNS	denotes	PCRs
T7513	24680-24686	VBD	denotes	showed
T7523	24687-24688	DT	denotes	a
T7524	24689-24703	NN	denotes	downregulation
T7525	24704-24706	IN	denotes	of
T7526	24707-24715	NN	denotes	Slc39a10
T7527	24716-24726	NN	denotes	expression
T7528	24727-24729	IN	denotes	in
T7529	24730-24736	NNS	denotes	livers
T7530	24737-24739	IN	denotes	of
T7531	24740-24748	NN	denotes	Mtf1loxP
T7532	24749-24753	NNS	denotes	mice
T7533	24754-24758	IN	denotes	upon
T7534	24759-24766	NN	denotes	cadmium
T7535	24767-24775	NN	denotes	exposure
T7536	24775-24776	.	denotes	.
T7537	24776-24942	sentence	denotes	In samples from Mtf1Mx-cre mice, the basal expression was significantly increased; cadmium treatment still resulted in a decrease of Slc39a10 expression (Figure 5a).
T7538	24777-24779	IN	denotes	In
T7540	24780-24787	NNS	denotes	samples
T7541	24788-24792	IN	denotes	from
T7542	24793-24799	NN	denotes	Mtf1Mx
T7544	24799-24800	HYPH	denotes	-
T7543	24800-24803	NN	denotes	cre
T7545	24804-24808	NNS	denotes	mice
T7546	24808-24810	,	denotes	,
T7547	24810-24813	DT	denotes	the
T7549	24814-24819	JJ	denotes	basal
T7548	24820-24830	NN	denotes	expression
T7550	24831-24834	VBD	denotes	was
T7551	24835-24848	RB	denotes	significantly
T7539	24849-24858	VBN	denotes	increased
T7553	24858-24859	:	denotes	;
T7554	24860-24867	NN	denotes	cadmium
T7555	24868-24877	NN	denotes	treatment
T7556	24878-24883	RB	denotes	still
T7552	24884-24892	VBD	denotes	resulted
T7557	24893-24895	IN	denotes	in
T7558	24896-24897	DT	denotes	a
T7559	24898-24906	NN	denotes	decrease
T7560	24907-24909	IN	denotes	of
T7561	24910-24918	NN	denotes	Slc39a10
T7562	24919-24929	NN	denotes	expression
T7563	24930-24931	-LRB-	denotes	(
T7565	24931-24937	NN	denotes	Figure
T7564	24938-24940	NN	denotes	5a
T7566	24940-24941	-RRB-	denotes	)
T7567	24941-24942	.	denotes	.
T7568	24942-25136	sentence	denotes	It cannot be judged by this experiment whether the degree of cadmium-induced reduction of Slc39a10 transcription was identical for Mtf1Mx-cre and Mtf1loxP mice or lower in the absence of MTF-1.
T7569	24943-24945	PRP	denotes	It
T7571	24946-24949	MD	denotes	can
T7572	24949-24952	RB	denotes	not
T7573	24953-24955	VB	denotes	be
T7570	24956-24962	VBN	denotes	judged
T7574	24963-24965	IN	denotes	by
T7575	24966-24970	DT	denotes	this
T7576	24971-24981	NN	denotes	experiment
T7577	24982-24989	IN	denotes	whether
T7579	24990-24993	DT	denotes	the
T7580	24994-25000	NN	denotes	degree
T7581	25001-25003	IN	denotes	of
T7582	25004-25011	NN	denotes	cadmium
T7584	25011-25012	HYPH	denotes	-
T7583	25012-25019	VBN	denotes	induced
T7585	25020-25029	NN	denotes	reduction
T7586	25030-25032	IN	denotes	of
T7587	25033-25041	NN	denotes	Slc39a10
T7588	25042-25055	NN	denotes	transcription
T7578	25056-25059	VBD	denotes	was
T7589	25060-25069	JJ	denotes	identical
T7590	25070-25073	IN	denotes	for
T7591	25074-25080	NN	denotes	Mtf1Mx
T7593	25080-25081	HYPH	denotes	-
T7592	25081-25084	NN	denotes	cre
T7595	25085-25088	CC	denotes	and
T7596	25089-25097	NN	denotes	Mtf1loxP
T7594	25098-25102	NNS	denotes	mice
T7597	25103-25105	CC	denotes	or
T7598	25106-25111	JJR	denotes	lower
T7599	25112-25114	IN	denotes	in
T7600	25115-25118	DT	denotes	the
T7601	25119-25126	NN	denotes	absence
T7602	25127-25129	IN	denotes	of
T7603	25130-25133	NN	denotes	MTF
T7604	25133-25134	HYPH	denotes	-
T7605	25134-25135	CD	denotes	1
T7606	25135-25136	.	denotes	.
T7607	25136-25331	sentence	denotes	In microarray analysis, the degree of the downregulation was either comparable to the one in control livers or lower, depending on the considered Affymetrix GeneChip® probe set (data not shown).
T7608	25137-25139	IN	denotes	In
T7610	25140-25150	NN	denotes	microarray
T7611	25151-25159	NN	denotes	analysis
T7612	25159-25161	,	denotes	,
T7613	25161-25164	DT	denotes	the
T7614	25165-25171	NN	denotes	degree
T7615	25172-25174	IN	denotes	of
T7616	25175-25178	DT	denotes	the
T7617	25179-25193	NN	denotes	downregulation
T7609	25194-25197	VBD	denotes	was
T7618	25198-25204	CC	denotes	either
T7619	25205-25215	JJ	denotes	comparable
T7620	25216-25218	IN	denotes	to
T7621	25219-25222	DT	denotes	the
T7622	25223-25226	NN	denotes	one
T7623	25227-25229	IN	denotes	in
T7624	25230-25237	NN	denotes	control
T7625	25238-25244	NNS	denotes	livers
T7626	25245-25247	CC	denotes	or
T7627	25248-25253	JJR	denotes	lower
T7628	25253-25255	,	denotes	,
T7629	25255-25264	VBG	denotes	depending
T7630	25265-25267	IN	denotes	on
T7631	25268-25271	DT	denotes	the
T7633	25272-25282	VBN	denotes	considered
T7634	25283-25293	NNP	denotes	Affymetrix
T7635	25294-25302	NNP	denotes	GeneChip
T7636	25302-25303	SYM	denotes	®
T7637	25304-25309	NN	denotes	probe
T7632	25310-25313	NN	denotes	set
T7638	25314-25315	-LRB-	denotes	(
T7640	25315-25319	NNS	denotes	data
T7641	25320-25323	RB	denotes	not
T7639	25324-25329	VBN	denotes	shown
T7642	25329-25330	-RRB-	denotes	)
T7643	25330-25331	.	denotes	.
T7644	25331-25426	sentence	denotes	The results indicate that MTF-1 is involved in repression of the basal expression of Slc39a10.
T7645	25332-25335	DT	denotes	The
T7646	25336-25343	NNS	denotes	results
T7647	25344-25352	VBP	denotes	indicate
T7648	25353-25357	IN	denotes	that
T7650	25358-25361	NN	denotes	MTF
T7651	25361-25362	HYPH	denotes	-
T7652	25362-25363	CD	denotes	1
T7653	25364-25366	VBZ	denotes	is
T7649	25367-25375	VBN	denotes	involved
T7654	25376-25378	IN	denotes	in
T7655	25379-25389	NN	denotes	repression
T7656	25390-25392	IN	denotes	of
T7657	25393-25396	DT	denotes	the
T7659	25397-25402	JJ	denotes	basal
T7658	25403-25413	NN	denotes	expression
T7660	25414-25416	IN	denotes	of
T7661	25417-25425	NN	denotes	Slc39a10
T7662	25425-25426	.	denotes	.
T7663	25426-25540	sentence	denotes	It might also participate in the cadmium response of this gene, but it is apparently not exclusively responsible.
T7664	25427-25429	PRP	denotes	It
T7666	25430-25435	MD	denotes	might
T7667	25436-25440	RB	denotes	also
T7665	25441-25452	VB	denotes	participate
T7668	25453-25455	IN	denotes	in
T7669	25456-25459	DT	denotes	the
T7671	25460-25467	NN	denotes	cadmium
T7670	25468-25476	NN	denotes	response
T7672	25477-25479	IN	denotes	of
T7673	25480-25484	DT	denotes	this
T7674	25485-25489	NN	denotes	gene
T7675	25489-25491	,	denotes	,
T7676	25491-25494	CC	denotes	but
T7677	25495-25497	PRP	denotes	it
T7678	25498-25500	VBZ	denotes	is
T7679	25501-25511	RB	denotes	apparently
T7680	25512-25515	RB	denotes	not
T7681	25516-25527	RB	denotes	exclusively
T7682	25528-25539	JJ	denotes	responsible
T7683	25539-25540	.	denotes	.
T7684	25540-25713	sentence	denotes	One MRE core consensus sequence was found just upstream of the mouse Slc39a10 transcription start (MRE1, −21 bp), another one directly downstream (MRE2, +17 bp; Figure 5b).
T7685	25541-25544	CD	denotes	One
T7687	25545-25548	NN	denotes	MRE
T7688	25549-25553	NN	denotes	core
T7689	25554-25563	NN	denotes	consensus
T7686	25564-25572	NN	denotes	sequence
T7691	25573-25576	VBD	denotes	was
T7690	25577-25582	VBN	denotes	found
T7693	25583-25587	RB	denotes	just
T7694	25588-25596	RB	denotes	upstream
T7695	25597-25599	IN	denotes	of
T7696	25600-25603	DT	denotes	the
T7698	25604-25609	NN	denotes	mouse
T7699	25610-25618	NN	denotes	Slc39a10
T7700	25619-25632	NN	denotes	transcription
T7697	25633-25638	NN	denotes	start
T7701	25639-25640	-LRB-	denotes	(
T7703	25640-25644	NN	denotes	MRE1
T7704	25644-25646	,	denotes	,
T7705	25646-25647	SYM	denotes	−
T7706	25647-25649	CD	denotes	21
T7702	25650-25652	NN	denotes	bp
T7707	25652-25653	-RRB-	denotes	)
T7708	25653-25655	,	denotes	,
T7709	25655-25662	DT	denotes	another
T7692	25663-25666	NN	denotes	one
T7710	25667-25675	RB	denotes	directly
T7711	25676-25686	JJ	denotes	downstream
T7712	25687-25688	-LRB-	denotes	(
T7714	25688-25692	NN	denotes	MRE2
T7715	25692-25694	,	denotes	,
T7716	25694-25695	SYM	denotes	+
T7717	25695-25697	CD	denotes	17
T7718	25698-25700	NN	denotes	bp
T7719	25700-25701	:	denotes	;
T7720	25702-25708	NN	denotes	Figure
T7713	25709-25711	NN	denotes	5b
T7721	25711-25712	-RRB-	denotes	)
T7722	25712-25713	.	denotes	.
T7723	25713-25909	sentence	denotes	Specific binding of MTF-1 was observed in EMSA analysis for MRE2 with liver protein extract from an Mtf1loxP but not from an Mtf1Mx-cre mouse, while no binding was detected with MRE1 (Figure 5c).
T7724	25714-25722	JJ	denotes	Specific
T7725	25723-25730	NN	denotes	binding
T7727	25731-25733	IN	denotes	of
T7728	25734-25737	NN	denotes	MTF
T7729	25737-25738	HYPH	denotes	-
T7730	25738-25739	CD	denotes	1
T7731	25740-25743	VBD	denotes	was
T7726	25744-25752	VBN	denotes	observed
T7732	25753-25755	IN	denotes	in
T7733	25756-25760	NN	denotes	EMSA
T7734	25761-25769	NN	denotes	analysis
T7735	25770-25773	IN	denotes	for
T7736	25774-25778	NN	denotes	MRE2
T7737	25779-25783	IN	denotes	with
T7738	25784-25789	NN	denotes	liver
T7740	25790-25797	NN	denotes	protein
T7739	25798-25805	NN	denotes	extract
T7741	25806-25810	IN	denotes	from
T7742	25811-25813	DT	denotes	an
T7743	25814-25822	NN	denotes	Mtf1loxP
T7744	25823-25826	CC	denotes	but
T7745	25827-25830	RB	denotes	not
T7746	25831-25835	IN	denotes	from
T7747	25836-25838	DT	denotes	an
T7749	25839-25845	NN	denotes	Mtf1Mx
T7751	25845-25846	HYPH	denotes	-
T7750	25846-25849	NN	denotes	cre
T7748	25850-25855	NN	denotes	mouse
T7752	25855-25857	,	denotes	,
T7753	25857-25862	IN	denotes	while
T7755	25863-25865	DT	denotes	no
T7756	25866-25873	NN	denotes	binding
T7757	25874-25877	VBD	denotes	was
T7754	25878-25886	VBN	denotes	detected
T7758	25887-25891	IN	denotes	with
T7759	25892-25896	NN	denotes	MRE1
T7760	25897-25898	-LRB-	denotes	(
T7762	25898-25904	NN	denotes	Figure
T7761	25905-25907	NN	denotes	5c
T7763	25907-25908	-RRB-	denotes	)
T7764	25908-25909	.	denotes	.
T8110	25911-25918	NN	denotes	Cadmium
T8112	25918-25919	HYPH	denotes	-
T8111	25919-25929	JJ	denotes	responsive
T8114	25929-25931	,	denotes	,
T8115	25931-25934	NN	denotes	MTF
T8117	25934-25935	HYPH	denotes	-
T8118	25935-25936	CD	denotes	1
T8119	25936-25937	HYPH	denotes	-
T8116	25937-25948	JJ	denotes	independent
T8113	25949-25954	NNS	denotes	genes
T8120	25954-26202	sentence	denotes	Finally, we also identified a number of cadmium-responsive genes that were independent of MTF-1 presence, by comparing the probe array data of all cadmium-treated mice with the data of all mock-treated mice, irrespective of the genotype (Table 2).
T8121	25955-25962	RB	denotes	Finally
T8123	25962-25964	,	denotes	,
T8124	25964-25966	PRP	denotes	we
T8125	25967-25971	RB	denotes	also
T8122	25972-25982	VBD	denotes	identified
T8126	25983-25984	DT	denotes	a
T8127	25985-25991	NN	denotes	number
T8128	25992-25994	IN	denotes	of
T8129	25995-26002	NN	denotes	cadmium
T8131	26002-26003	HYPH	denotes	-
T8130	26003-26013	JJ	denotes	responsive
T8132	26014-26019	NNS	denotes	genes
T8133	26020-26024	WDT	denotes	that
T8134	26025-26029	VBD	denotes	were
T8135	26030-26041	JJ	denotes	independent
T8136	26042-26044	IN	denotes	of
T8137	26045-26048	NN	denotes	MTF
T8139	26048-26049	HYPH	denotes	-
T8140	26049-26050	CD	denotes	1
T8138	26051-26059	NN	denotes	presence
T8141	26059-26061	,	denotes	,
T8142	26061-26063	IN	denotes	by
T8143	26064-26073	VBG	denotes	comparing
T8144	26074-26077	DT	denotes	the
T8146	26078-26083	NN	denotes	probe
T8147	26084-26089	NN	denotes	array
T8145	26090-26094	NNS	denotes	data
T8148	26095-26097	IN	denotes	of
T8149	26098-26101	DT	denotes	all
T8151	26102-26109	NN	denotes	cadmium
T8153	26109-26110	HYPH	denotes	-
T8152	26110-26117	VBN	denotes	treated
T8150	26118-26122	NNS	denotes	mice
T8154	26123-26127	IN	denotes	with
T8155	26128-26131	DT	denotes	the
T8156	26132-26136	NNS	denotes	data
T8157	26137-26139	IN	denotes	of
T8158	26140-26143	DT	denotes	all
T8160	26144-26148	JJ	denotes	mock
T8162	26148-26149	HYPH	denotes	-
T8161	26149-26156	VBN	denotes	treated
T8159	26157-26161	NNS	denotes	mice
T8163	26161-26163	,	denotes	,
T8164	26163-26175	RB	denotes	irrespective
T8165	26176-26178	IN	denotes	of
T8166	26179-26182	DT	denotes	the
T8167	26183-26191	NN	denotes	genotype
T8168	26192-26193	-LRB-	denotes	(
T8169	26193-26198	NN	denotes	Table
T8170	26199-26200	CD	denotes	2
T8171	26200-26201	-RRB-	denotes	)
T8172	26201-26202	.	denotes	.
T8173	26202-26362	sentence	denotes	An at least 2-fold, reliable upregulation was observed after cadmium exposure for 31 probe sets corresponding to 21 different characterized genes (Table 2, a).
T8174	26203-26205	DT	denotes	An
T8176	26206-26208	RB	denotes	at
T8177	26209-26214	RBS	denotes	least
T8178	26215-26221	JJ	denotes	2-fold
T8179	26221-26223	,	denotes	,
T8180	26223-26231	JJ	denotes	reliable
T8175	26232-26244	NN	denotes	upregulation
T8182	26245-26248	VBD	denotes	was
T8181	26249-26257	VBN	denotes	observed
T8183	26258-26263	IN	denotes	after
T8184	26264-26271	NN	denotes	cadmium
T8185	26272-26280	NN	denotes	exposure
T8186	26281-26284	IN	denotes	for
T8187	26285-26287	CD	denotes	31
T8189	26288-26293	NN	denotes	probe
T8188	26294-26298	NNS	denotes	sets
T8190	26299-26312	VBG	denotes	corresponding
T8191	26313-26315	IN	denotes	to
T8192	26316-26318	CD	denotes	21
T8194	26319-26328	JJ	denotes	different
T8195	26329-26342	VBN	denotes	characterized
T8193	26343-26348	NNS	denotes	genes
T8196	26349-26350	-LRB-	denotes	(
T8198	26350-26355	NN	denotes	Table
T8199	26356-26357	CD	denotes	2
T8200	26357-26359	,	denotes	,
T8197	26359-26360	NN	denotes	a
T8201	26360-26361	-RRB-	denotes	)
T8202	26361-26362	.	denotes	.
T8203	26362-26480	sentence	denotes	For 2 probe sets corresponding to 2 characterized genes, an at least 2-fold downregulation was detected (Table 2, b).
T8204	26363-26366	IN	denotes	For
T8206	26367-26368	CD	denotes	2
T8208	26369-26374	NN	denotes	probe
T8207	26375-26379	NNS	denotes	sets
T8209	26380-26393	VBG	denotes	corresponding
T8210	26394-26396	IN	denotes	to
T8211	26397-26398	CD	denotes	2
T8213	26399-26412	VBN	denotes	characterized
T8212	26413-26418	NNS	denotes	genes
T8214	26418-26420	,	denotes	,
T8215	26420-26422	DT	denotes	an
T8217	26423-26425	RB	denotes	at
T8218	26426-26431	RBS	denotes	least
T8219	26432-26438	JJ	denotes	2-fold
T8216	26439-26453	NN	denotes	downregulation
T8220	26454-26457	VBD	denotes	was
T8205	26458-26466	VBN	denotes	detected
T8221	26467-26468	-LRB-	denotes	(
T8223	26468-26473	NN	denotes	Table
T8224	26474-26475	CD	denotes	2
T8225	26475-26477	,	denotes	,
T8222	26477-26478	NN	denotes	b
T8226	26478-26479	-RRB-	denotes	)
T8227	26479-26480	.	denotes	.
T8228	26480-26988	sentence	denotes	Several genes involved in the metabolism of the antioxidant glutathione were found to be upregulated by cadmium exposure, namely the genes encoding the catalytic subunit of glutamate-cysteine ligase (Gclc) that is the rate limiting enzyme in de novo synthesis of glutathione (45); glutathione reductase 1 (Gsr), the reducing enzyme for oxidized glutathione (45); and glutathione-S-transferase, mu 4 (Gstm4), which is a member of the glutathione-S-transferase supergene family of detoxification enzymes (45).
T8229	26481-26488	JJ	denotes	Several
T8230	26489-26494	NNS	denotes	genes
T8232	26495-26503	VBN	denotes	involved
T8233	26504-26506	IN	denotes	in
T8234	26507-26510	DT	denotes	the
T8235	26511-26521	NN	denotes	metabolism
T8236	26522-26524	IN	denotes	of
T8237	26525-26528	DT	denotes	the
T8239	26529-26540	NN	denotes	antioxidant
T8238	26541-26552	NN	denotes	glutathione
T8240	26553-26557	VBD	denotes	were
T8231	26558-26563	VBN	denotes	found
T8241	26564-26566	TO	denotes	to
T8243	26567-26569	VB	denotes	be
T8242	26570-26581	VBN	denotes	upregulated
T8244	26582-26584	IN	denotes	by
T8245	26585-26592	NN	denotes	cadmium
T8246	26593-26601	NN	denotes	exposure
T8247	26601-26603	,	denotes	,
T8248	26603-26609	RB	denotes	namely
T8250	26610-26613	DT	denotes	the
T8249	26614-26619	NNS	denotes	genes
T8251	26620-26628	VBG	denotes	encoding
T8252	26629-26632	DT	denotes	the
T8254	26633-26642	JJ	denotes	catalytic
T8253	26643-26650	NN	denotes	subunit
T8255	26651-26653	IN	denotes	of
T8256	26654-26663	NN	denotes	glutamate
T8258	26663-26664	HYPH	denotes	-
T8259	26664-26672	NN	denotes	cysteine
T8257	26673-26679	NN	denotes	ligase
T8260	26680-26681	-LRB-	denotes	(
T8261	26681-26685	NN	denotes	Gclc
T8262	26685-26686	-RRB-	denotes	)
T8263	26687-26691	WDT	denotes	that
T8264	26692-26694	VBZ	denotes	is
T8265	26695-26698	DT	denotes	the
T8267	26699-26703	NN	denotes	rate
T8268	26704-26712	VBG	denotes	limiting
T8266	26713-26719	NN	denotes	enzyme
T8269	26720-26722	IN	denotes	in
T8270	26723-26725	FW	denotes	de
T8271	26726-26730	FW	denotes	novo
T8272	26731-26740	NN	denotes	synthesis
T8273	26741-26743	IN	denotes	of
T8274	26744-26755	NN	denotes	glutathione
T8275	26756-26757	-LRB-	denotes	(
T8276	26757-26759	CD	denotes	45
T8277	26759-26760	-RRB-	denotes	)
T8278	26760-26761	:	denotes	;
T8279	26762-26773	NN	denotes	glutathione
T8280	26774-26783	NN	denotes	reductase
T8281	26784-26785	CD	denotes	1
T8282	26786-26787	-LRB-	denotes	(
T8283	26787-26790	NN	denotes	Gsr
T8284	26790-26791	-RRB-	denotes	)
T8285	26791-26793	,	denotes	,
T8286	26793-26796	DT	denotes	the
T8288	26797-26805	VBG	denotes	reducing
T8287	26806-26812	NN	denotes	enzyme
T8289	26813-26816	IN	denotes	for
T8290	26817-26825	VBN	denotes	oxidized
T8291	26826-26837	NN	denotes	glutathione
T8292	26838-26839	-LRB-	denotes	(
T8293	26839-26841	CD	denotes	45
T8294	26841-26842	-RRB-	denotes	)
T8295	26842-26843	:	denotes	;
T8296	26844-26847	CC	denotes	and
T8297	26848-26859	NN	denotes	glutathione
T8299	26859-26860	HYPH	denotes	-
T8300	26860-26861	NN	denotes	S
T8301	26861-26862	HYPH	denotes	-
T8298	26862-26873	NN	denotes	transferase
T8302	26873-26875	,	denotes	,
T8303	26875-26877	NN	denotes	mu
T8305	26878-26879	CD	denotes	4
T8306	26880-26881	-LRB-	denotes	(
T8304	26881-26886	NN	denotes	Gstm4
T8307	26886-26887	-RRB-	denotes	)
T8308	26887-26889	,	denotes	,
T8309	26889-26894	WDT	denotes	which
T8310	26895-26897	VBZ	denotes	is
T8311	26898-26899	DT	denotes	a
T8312	26900-26906	NN	denotes	member
T8313	26907-26909	IN	denotes	of
T8314	26910-26913	DT	denotes	the
T8316	26914-26925	NN	denotes	glutathione
T8318	26925-26926	HYPH	denotes	-
T8319	26926-26927	NN	denotes	S
T8320	26927-26928	HYPH	denotes	-
T8317	26928-26939	NN	denotes	transferase
T8321	26940-26949	NN	denotes	supergene
T8315	26950-26956	NN	denotes	family
T8322	26957-26959	IN	denotes	of
T8323	26960-26974	NN	denotes	detoxification
T8324	26975-26982	NNS	denotes	enzymes
T8325	26983-26984	-LRB-	denotes	(
T8326	26984-26986	CD	denotes	45
T8327	26986-26987	-RRB-	denotes	)
T8328	26987-26988	.	denotes	.
T8329	26988-27081	sentence	denotes	In all of these cases, induction was confirmed by semiquantitative RT–PCRs (data not shown).
T8330	26989-26991	IN	denotes	In
T8332	26992-26995	DT	denotes	all
T8333	26996-26998	IN	denotes	of
T8334	26999-27004	DT	denotes	these
T8335	27005-27010	NNS	denotes	cases
T8336	27010-27012	,	denotes	,
T8337	27012-27021	NN	denotes	induction
T8338	27022-27025	VBD	denotes	was
T8331	27026-27035	VBN	denotes	confirmed
T8339	27036-27038	IN	denotes	by
T8340	27039-27055	JJ	denotes	semiquantitative
T8342	27056-27058	NN	denotes	RT
T8343	27058-27059	HYPH	denotes	–
T8341	27059-27063	NNS	denotes	PCRs
T8344	27064-27065	-LRB-	denotes	(
T8346	27065-27069	NNS	denotes	data
T8347	27070-27073	RB	denotes	not
T8345	27074-27079	VBN	denotes	shown
T8348	27079-27080	-RRB-	denotes	)
T8349	27080-27081	.	denotes	.
T8350	27081-27236	sentence	denotes	Gclc, also referred to as heavy chain subunit of gamma-glutamylcysteine synthetase (Ggcs-hc), had been discussed previously as a target gene of MTF-1 (6).
T8351	27082-27086	NN	denotes	Gclc
T8353	27086-27088	,	denotes	,
T8354	27088-27092	RB	denotes	also
T8355	27093-27101	VBN	denotes	referred
T8356	27102-27104	IN	denotes	to
T8357	27105-27107	IN	denotes	as
T8358	27108-27113	JJ	denotes	heavy
T8359	27114-27119	NN	denotes	chain
T8360	27120-27127	NN	denotes	subunit
T8361	27128-27130	IN	denotes	of
T8362	27131-27136	NN	denotes	gamma
T8364	27136-27137	HYPH	denotes	-
T8363	27137-27153	NN	denotes	glutamylcysteine
T8365	27154-27164	NN	denotes	synthetase
T8366	27165-27166	-LRB-	denotes	(
T8367	27166-27170	NN	denotes	Ggcs
T8369	27170-27171	HYPH	denotes	-
T8368	27171-27173	NN	denotes	hc
T8370	27173-27174	-RRB-	denotes	)
T8371	27174-27176	,	denotes	,
T8372	27176-27179	VBD	denotes	had
T8373	27180-27184	VBN	denotes	been
T8352	27185-27194	VBN	denotes	discussed
T8374	27195-27205	RB	denotes	previously
T8375	27206-27208	IN	denotes	as
T8376	27209-27210	DT	denotes	a
T8378	27211-27217	NN	denotes	target
T8377	27218-27222	NN	denotes	gene
T8379	27223-27225	IN	denotes	of
T8380	27226-27229	NN	denotes	MTF
T8381	27229-27230	HYPH	denotes	-
T8382	27230-27231	CD	denotes	1
T8383	27232-27233	-LRB-	denotes	(
T8384	27233-27234	CD	denotes	6
T8385	27234-27235	-RRB-	denotes	)
T8386	27235-27236	.	denotes	.
T8387	27236-27361	sentence	denotes	Our expression data indicate that Gclc is induced by cadmium but, at least in the adult mouse liver, not dependent on MTF-1.
T8388	27237-27240	PRP$	denotes	Our
T8390	27241-27251	NN	denotes	expression
T8389	27252-27256	NNS	denotes	data
T8391	27257-27265	VBP	denotes	indicate
T8392	27266-27270	IN	denotes	that
T8394	27271-27275	NN	denotes	Gclc
T8395	27276-27278	VBZ	denotes	is
T8393	27279-27286	VBN	denotes	induced
T8396	27287-27289	IN	denotes	by
T8397	27290-27297	NN	denotes	cadmium
T8398	27298-27301	CC	denotes	but
T8399	27301-27303	,	denotes	,
T8400	27303-27305	RB	denotes	at
T8401	27306-27311	RBS	denotes	least
T8402	27312-27314	IN	denotes	in
T8404	27315-27318	DT	denotes	the
T8406	27319-27324	JJ	denotes	adult
T8407	27325-27330	NN	denotes	mouse
T8405	27331-27336	NN	denotes	liver
T8408	27336-27338	,	denotes	,
T8409	27338-27341	RB	denotes	not
T8403	27342-27351	JJ	denotes	dependent
T8410	27352-27354	IN	denotes	on
T8411	27355-27358	NN	denotes	MTF
T8412	27358-27359	HYPH	denotes	-
T8413	27359-27360	CD	denotes	1
T8414	27360-27361	.	denotes	.
T8415	27361-27711	sentence	denotes	To analyze the role of the glutathione system in the cellular cadmium response, mouse embryonic fibroblasts with and without functional Mtf1 were treated with cadmium in combination with BSO, a specific inhibitor of glutamate-cysteine ligase (31), and cell viability was assessed by a colorimetric assay based on the tetrazolium salt MTT (Figure 6).
T8416	27362-27364	TO	denotes	To
T8417	27365-27372	VB	denotes	analyze
T8419	27373-27376	DT	denotes	the
T8420	27377-27381	NN	denotes	role
T8421	27382-27384	IN	denotes	of
T8422	27385-27388	DT	denotes	the
T8424	27389-27400	NN	denotes	glutathione
T8423	27401-27407	NN	denotes	system
T8425	27408-27410	IN	denotes	in
T8426	27411-27414	DT	denotes	the
T8428	27415-27423	JJ	denotes	cellular
T8429	27424-27431	NN	denotes	cadmium
T8427	27432-27440	NN	denotes	response
T8430	27440-27442	,	denotes	,
T8431	27442-27447	NN	denotes	mouse
T8433	27448-27457	JJ	denotes	embryonic
T8432	27458-27469	NNS	denotes	fibroblasts
T8434	27470-27474	IN	denotes	with
T8435	27475-27478	CC	denotes	and
T8436	27479-27486	IN	denotes	without
T8437	27487-27497	JJ	denotes	functional
T8438	27498-27502	NN	denotes	Mtf1
T8439	27503-27507	VBD	denotes	were
T8418	27508-27515	VBN	denotes	treated
T8440	27516-27520	IN	denotes	with
T8441	27521-27528	NN	denotes	cadmium
T8442	27529-27531	IN	denotes	in
T8443	27532-27543	NN	denotes	combination
T8444	27544-27548	IN	denotes	with
T8445	27549-27552	NN	denotes	BSO
T8446	27552-27554	,	denotes	,
T8447	27554-27555	DT	denotes	a
T8449	27556-27564	JJ	denotes	specific
T8448	27565-27574	NN	denotes	inhibitor
T8450	27575-27577	IN	denotes	of
T8451	27578-27587	NN	denotes	glutamate
T8453	27587-27588	HYPH	denotes	-
T8452	27588-27596	NN	denotes	cysteine
T8454	27597-27603	NN	denotes	ligase
T8455	27604-27605	-LRB-	denotes	(
T8456	27605-27607	CD	denotes	31
T8457	27607-27608	-RRB-	denotes	)
T8458	27608-27610	,	denotes	,
T8459	27610-27613	CC	denotes	and
T8460	27614-27618	NN	denotes	cell
T8461	27619-27628	NN	denotes	viability
T8463	27629-27632	VBD	denotes	was
T8462	27633-27641	VBN	denotes	assessed
T8464	27642-27644	IN	denotes	by
T8465	27645-27646	DT	denotes	a
T8467	27647-27659	JJ	denotes	colorimetric
T8466	27660-27665	NN	denotes	assay
T8468	27666-27671	VBN	denotes	based
T8469	27672-27674	IN	denotes	on
T8470	27675-27678	DT	denotes	the
T8472	27679-27690	NN	denotes	tetrazolium
T8471	27691-27695	NN	denotes	salt
T8473	27696-27699	NN	denotes	MTT
T8474	27700-27701	-LRB-	denotes	(
T8475	27701-27707	NN	denotes	Figure
T8476	27708-27709	CD	denotes	6
T8477	27709-27710	-RRB-	denotes	)
T8478	27710-27711	.	denotes	.
T8479	27711-27820	sentence	denotes	Increasing concentrations of BSO or cadmium alone were to some extent cytotoxic for the examined cell lines.
T8480	27712-27722	VBG	denotes	Increasing
T8481	27723-27737	NNS	denotes	concentrations
T8483	27738-27740	IN	denotes	of
T8484	27741-27744	NN	denotes	BSO
T8485	27745-27747	CC	denotes	or
T8486	27748-27755	NN	denotes	cadmium
T8487	27756-27761	RB	denotes	alone
T8482	27762-27766	VBD	denotes	were
T8488	27767-27769	IN	denotes	to
T8489	27770-27774	DT	denotes	some
T8490	27775-27781	NN	denotes	extent
T8491	27782-27791	JJ	denotes	cytotoxic
T8492	27792-27795	IN	denotes	for
T8493	27796-27799	DT	denotes	the
T8495	27800-27808	VBN	denotes	examined
T8496	27809-27813	NN	denotes	cell
T8494	27814-27819	NNS	denotes	lines
T8497	27819-27820	.	denotes	.
T8498	27820-28056	sentence	denotes	Treatment with both BSO and cadmium resulted in an enhanced lethality particularly for the cells without functional Mtf1, indicating that a depletion of glutathione together with a lack of Mtf1 impair an efficient anti-cadmium defense.
T8499	27821-27830	NN	denotes	Treatment
T8501	27831-27835	IN	denotes	with
T8502	27836-27840	CC	denotes	both
T8503	27841-27844	NN	denotes	BSO
T8504	27845-27848	CC	denotes	and
T8505	27849-27856	NN	denotes	cadmium
T8500	27857-27865	VBD	denotes	resulted
T8506	27866-27868	IN	denotes	in
T8507	27869-27871	DT	denotes	an
T8509	27872-27880	VBN	denotes	enhanced
T8508	27881-27890	NN	denotes	lethality
T8510	27891-27903	RB	denotes	particularly
T8511	27904-27907	IN	denotes	for
T8512	27908-27911	DT	denotes	the
T8513	27912-27917	NNS	denotes	cells
T8514	27918-27925	IN	denotes	without
T8515	27926-27936	JJ	denotes	functional
T8516	27937-27941	NN	denotes	Mtf1
T8517	27941-27943	,	denotes	,
T8518	27943-27953	VBG	denotes	indicating
T8519	27954-27958	IN	denotes	that
T8521	27959-27960	DT	denotes	a
T8522	27961-27970	NN	denotes	depletion
T8523	27971-27973	IN	denotes	of
T8524	27974-27985	NN	denotes	glutathione
T8525	27986-27994	RB	denotes	together
T8526	27995-27999	IN	denotes	with
T8527	28000-28001	DT	denotes	a
T8528	28002-28006	NN	denotes	lack
T8529	28007-28009	IN	denotes	of
T8530	28010-28014	NN	denotes	Mtf1
T8520	28015-28021	VBP	denotes	impair
T8531	28022-28024	DT	denotes	an
T8533	28025-28034	JJ	denotes	efficient
T8534	28035-28047	JJ	denotes	anti-cadmium
T8532	28048-28055	NN	denotes	defense
T8535	28055-28056	.	denotes	.
T8536	28056-28193	sentence	denotes	Thus, adequate glutathione supply as well as MTF-1 and its target genes are essential for the survival of the cell under cadmium stress.
T8537	28057-28061	RB	denotes	Thus
T8539	28061-28063	,	denotes	,
T8540	28063-28071	JJ	denotes	adequate
T8542	28072-28083	NN	denotes	glutathione
T8541	28084-28090	NN	denotes	supply
T8543	28091-28093	RB	denotes	as
T8545	28094-28098	RB	denotes	well
T8544	28099-28101	IN	denotes	as
T8546	28102-28105	NN	denotes	MTF
T8547	28105-28106	HYPH	denotes	-
T8548	28106-28107	CD	denotes	1
T8549	28108-28111	CC	denotes	and
T8550	28112-28115	PRP$	denotes	its
T8552	28116-28122	NN	denotes	target
T8551	28123-28128	NNS	denotes	genes
T8538	28129-28132	VBP	denotes	are
T8553	28133-28142	JJ	denotes	essential
T8554	28143-28146	IN	denotes	for
T8555	28147-28150	DT	denotes	the
T8556	28151-28159	NN	denotes	survival
T8557	28160-28162	IN	denotes	of
T8558	28163-28166	DT	denotes	the
T8559	28167-28171	NN	denotes	cell
T8560	28172-28177	IN	denotes	under
T8561	28178-28185	NN	denotes	cadmium
T8562	28186-28192	NN	denotes	stress
T8563	28192-28193	.	denotes	.
T8564	28193-28646	sentence	denotes	Besides genes related to the glutathione pathway, several other stress-related genes were upregulated upon cadmium exposure, including genes for thioredoxin reductase 1 (Txnrd1), one of the reducing enzymes of the antioxidant thioredoxin (46); KDEL endoplasmic reticulum protein retention receptor 2 (Kdelr2) participating in ER stress response (47); and the anti-apoptotic Bcl2-associated athanogene 3 (Bag3) involved in stress-induced apoptosis (48).
T8565	28194-28201	IN	denotes	Besides
T8567	28202-28207	NNS	denotes	genes
T8568	28208-28215	VBN	denotes	related
T8569	28216-28218	IN	denotes	to
T8570	28219-28222	DT	denotes	the
T8572	28223-28234	NN	denotes	glutathione
T8571	28235-28242	NN	denotes	pathway
T8573	28242-28244	,	denotes	,
T8574	28244-28251	JJ	denotes	several
T8576	28252-28257	JJ	denotes	other
T8577	28258-28264	NN	denotes	stress
T8579	28264-28265	HYPH	denotes	-
T8578	28265-28272	VBN	denotes	related
T8575	28273-28278	NNS	denotes	genes
T8580	28279-28283	VBD	denotes	were
T8566	28284-28295	VBN	denotes	upregulated
T8581	28296-28300	IN	denotes	upon
T8582	28301-28308	NN	denotes	cadmium
T8583	28309-28317	NN	denotes	exposure
T8584	28317-28319	,	denotes	,
T8585	28319-28328	VBG	denotes	including
T8586	28329-28334	NNS	denotes	genes
T8587	28335-28338	IN	denotes	for
T8588	28339-28350	NN	denotes	thioredoxin
T8589	28351-28360	NN	denotes	reductase
T8590	28361-28362	CD	denotes	1
T8591	28363-28364	-LRB-	denotes	(
T8592	28364-28370	NN	denotes	Txnrd1
T8593	28370-28371	-RRB-	denotes	)
T8594	28371-28373	,	denotes	,
T8595	28373-28376	CD	denotes	one
T8596	28377-28379	IN	denotes	of
T8597	28380-28383	DT	denotes	the
T8599	28384-28392	VBG	denotes	reducing
T8598	28393-28400	NNS	denotes	enzymes
T8600	28401-28403	IN	denotes	of
T8601	28404-28407	DT	denotes	the
T8603	28408-28419	NN	denotes	antioxidant
T8602	28420-28431	NN	denotes	thioredoxin
T8604	28432-28433	-LRB-	denotes	(
T8605	28433-28435	CD	denotes	46
T8606	28435-28436	-RRB-	denotes	)
T8607	28436-28437	:	denotes	;
T8608	28438-28442	NN	denotes	KDEL
T8610	28443-28454	JJ	denotes	endoplasmic
T8611	28455-28464	NN	denotes	reticulum
T8612	28465-28472	NN	denotes	protein
T8613	28473-28482	NN	denotes	retention
T8609	28483-28491	NN	denotes	receptor
T8614	28492-28493	CD	denotes	2
T8615	28494-28495	-LRB-	denotes	(
T8616	28495-28501	NN	denotes	Kdelr2
T8617	28501-28502	-RRB-	denotes	)
T8618	28503-28516	VBG	denotes	participating
T8619	28517-28519	IN	denotes	in
T8620	28520-28522	NN	denotes	ER
T8622	28523-28529	NN	denotes	stress
T8621	28530-28538	NN	denotes	response
T8623	28539-28540	-LRB-	denotes	(
T8624	28540-28542	CD	denotes	47
T8625	28542-28543	-RRB-	denotes	)
T8626	28543-28544	:	denotes	;
T8627	28545-28548	CC	denotes	and
T8628	28549-28552	DT	denotes	the
T8630	28553-28567	JJ	denotes	anti-apoptotic
T8631	28568-28572	NN	denotes	Bcl2
T8632	28572-28573	HYPH	denotes	-
T8633	28573-28583	VBN	denotes	associated
T8629	28584-28594	NN	denotes	athanogene
T8634	28595-28596	CD	denotes	3
T8635	28597-28598	-LRB-	denotes	(
T8636	28598-28602	NN	denotes	Bag3
T8637	28602-28603	-RRB-	denotes	)
T8638	28604-28612	VBN	denotes	involved
T8639	28613-28615	IN	denotes	in
T8640	28616-28622	NN	denotes	stress
T8642	28622-28623	HYPH	denotes	-
T8641	28623-28630	VBN	denotes	induced
T8643	28631-28640	NN	denotes	apoptosis
T8644	28641-28642	-LRB-	denotes	(
T8645	28642-28644	CD	denotes	48
T8646	28644-28645	-RRB-	denotes	)
T8647	28645-28646	.	denotes	.
T9786	28659-28661	IN	denotes	In
T9788	28662-28666	DT	denotes	this
T9789	28667-28672	NN	denotes	study
T9790	28672-28674	,	denotes	,
T9791	28674-28675	DT	denotes	a
T9793	28676-28685	RB	denotes	virtually
T9794	28686-28694	JJ	denotes	complete
T9792	28695-28703	NN	denotes	deletion
T9795	28704-28706	IN	denotes	of
T9796	28707-28711	NN	denotes	Mtf1
T9797	28712-28714	IN	denotes	in
T9798	28715-28718	DT	denotes	the
T9799	28719-28724	NN	denotes	liver
T9800	28725-28727	IN	denotes	of
T9801	28728-28733	JJ	denotes	adult
T9803	28733-28735	,	denotes	,
T9804	28735-28737	NN	denotes	pI
T9806	28737-28738	HYPH	denotes	–
T9805	28738-28740	NN	denotes	pC
T9808	28740-28741	HYPH	denotes	-
T9807	28741-28748	VBN	denotes	induced
T9809	28749-28755	NN	denotes	Mtf1Mx
T9811	28755-28756	HYPH	denotes	-
T9810	28756-28759	NN	denotes	cre
T9802	28760-28764	NNS	denotes	mice
T9812	28765-28768	VBD	denotes	did
T9813	28769-28772	RB	denotes	not
T9814	28773-28783	RB	denotes	detectably
T9787	28784-28790	VB	denotes	affect
T9815	28791-28794	DT	denotes	the
T9816	28795-28804	NN	denotes	phenotype
T9817	28805-28807	IN	denotes	of
T9818	28808-28811	DT	denotes	the
T9820	28812-28822	JJ	denotes	respective
T9819	28823-28827	NNS	denotes	mice
T9821	28828-28833	IN	denotes	under
T9822	28834-28844	JJ	denotes	non-stress
T9823	28845-28855	NNS	denotes	conditions
T9824	28855-28857	,	denotes	,
T9825	28857-28867	VBG	denotes	confirming
T9826	28868-28872	IN	denotes	that
T9828	28873-28876	NN	denotes	MTF
T9829	28876-28877	HYPH	denotes	-
T9830	28877-28878	CD	denotes	1
T9827	28879-28881	VBZ	denotes	is
T9831	28882-28893	JJ	denotes	dispensable
T9832	28894-28896	IN	denotes	in
T9833	28897-28900	DT	denotes	the
T9835	28901-28906	JJ	denotes	adult
T9834	28907-28912	NN	denotes	liver
T9836	28913-28914	-LRB-	denotes	(
T9837	28914-28916	CD	denotes	26
T9838	28916-28917	-RRB-	denotes	)
T9839	28917-28919	,	denotes	,
T9840	28919-28921	IN	denotes	in
T9841	28922-28930	NN	denotes	contrast
T9842	28931-28933	IN	denotes	to
T9843	28934-28937	PRP$	denotes	its
T9845	28938-28947	JJ	denotes	essential
T9844	28948-28952	NN	denotes	role
T9846	28953-28955	IN	denotes	in
T9847	28956-28965	JJ	denotes	embryonic
T9849	28966-28971	NN	denotes	liver
T9848	28972-28983	NN	denotes	development
T9850	28984-28985	-LRB-	denotes	(
T9851	28985-28987	CD	denotes	23
T9852	28987-28988	-RRB-	denotes	)
T9853	28988-28989	.	denotes	.
T9854	28989-29137	sentence	denotes	The comparison of gene expression in livers of mock- or cadmium-treated Mtf1Mx-cre and Mtf1loxP mice revealed several MTF-1 target gene candidates.
T9855	28990-28993	DT	denotes	The
T9856	28994-29004	NN	denotes	comparison
T9858	29005-29007	IN	denotes	of
T9859	29008-29012	NN	denotes	gene
T9860	29013-29023	NN	denotes	expression
T9861	29024-29026	IN	denotes	in
T9862	29027-29033	NNS	denotes	livers
T9863	29034-29036	IN	denotes	of
T9864	29037-29041	JJ	denotes	mock
T9866	29041-29042	HYPH	denotes	-
T9867	29043-29045	CC	denotes	or
T9868	29046-29053	NN	denotes	cadmium
T9869	29053-29054	HYPH	denotes	-
T9865	29054-29061	VBN	denotes	treated
T9871	29062-29068	NN	denotes	Mtf1Mx
T9873	29068-29069	HYPH	denotes	-
T9872	29069-29072	NN	denotes	cre
T9874	29073-29076	CC	denotes	and
T9875	29077-29085	NN	denotes	Mtf1loxP
T9870	29086-29090	NNS	denotes	mice
T9857	29091-29099	VBD	denotes	revealed
T9876	29100-29107	JJ	denotes	several
T9878	29108-29111	NN	denotes	MTF
T9879	29111-29112	HYPH	denotes	-
T9880	29112-29113	CD	denotes	1
T9881	29114-29120	NN	denotes	target
T9882	29121-29125	NN	denotes	gene
T9877	29126-29136	NNS	denotes	candidates
T9883	29136-29137	.	denotes	.
T9884	29137-29344	sentence	denotes	Transcripts of the two stress-responsive metallothionein genes Mt1 and Mt2 were severely reduced, in support of a crucial role of MTF-1 for both basal and metal-induced expression of metallothioneins (4,6).
T9885	29138-29149	NNS	denotes	Transcripts
T9887	29150-29152	IN	denotes	of
T9888	29153-29156	DT	denotes	the
T9890	29157-29160	CD	denotes	two
T9891	29161-29167	NN	denotes	stress
T9893	29167-29168	HYPH	denotes	-
T9892	29168-29178	JJ	denotes	responsive
T9894	29179-29194	NN	denotes	metallothionein
T9889	29195-29200	NNS	denotes	genes
T9895	29201-29204	NN	denotes	Mt1
T9896	29205-29208	CC	denotes	and
T9897	29209-29212	NN	denotes	Mt2
T9898	29213-29217	VBD	denotes	were
T9899	29218-29226	RB	denotes	severely
T9886	29227-29234	VBN	denotes	reduced
T9900	29234-29236	,	denotes	,
T9901	29236-29238	IN	denotes	in
T9902	29239-29246	NN	denotes	support
T9903	29247-29249	IN	denotes	of
T9904	29250-29251	DT	denotes	a
T9906	29252-29259	JJ	denotes	crucial
T9905	29260-29264	NN	denotes	role
T9907	29265-29267	IN	denotes	of
T9908	29268-29271	NN	denotes	MTF
T9909	29271-29272	HYPH	denotes	-
T9910	29272-29273	CD	denotes	1
T9911	29274-29277	IN	denotes	for
T9912	29278-29282	CC	denotes	both
T9914	29283-29288	JJ	denotes	basal
T9915	29289-29292	CC	denotes	and
T9916	29293-29298	NN	denotes	metal
T9918	29298-29299	HYPH	denotes	-
T9917	29299-29306	VBN	denotes	induced
T9913	29307-29317	NN	denotes	expression
T9919	29318-29320	IN	denotes	of
T9920	29321-29337	NNS	denotes	metallothioneins
T9921	29338-29339	-LRB-	denotes	(
T9923	29339-29340	CD	denotes	4
T9924	29340-29341	,	denotes	,
T9922	29341-29342	CD	denotes	6
T9925	29342-29343	-RRB-	denotes	)
T9926	29343-29344	.	denotes	.
T9927	29344-29390	sentence	denotes	One of the newly found target genes is Sepw1.
T9928	29345-29348	CD	denotes	One
T9930	29349-29351	IN	denotes	of
T9931	29352-29355	DT	denotes	the
T9933	29356-29361	RB	denotes	newly
T9934	29362-29367	VBN	denotes	found
T9935	29368-29374	NN	denotes	target
T9932	29375-29380	NNS	denotes	genes
T9929	29381-29383	VBZ	denotes	is
T9936	29384-29389	NN	denotes	Sepw1
T9937	29389-29390	.	denotes	.
T9938	29390-29545	sentence	denotes	The exact molecular function of SEPW1 protein is unknown to date, but a role as antioxidant has been proposed due to its ability to bind glutathione (35).
T9939	29391-29394	DT	denotes	The
T9941	29395-29400	JJ	denotes	exact
T9942	29401-29410	JJ	denotes	molecular
T9940	29411-29419	NN	denotes	function
T9944	29420-29422	IN	denotes	of
T9945	29423-29428	NN	denotes	SEPW1
T9946	29429-29436	NN	denotes	protein
T9943	29437-29439	VBZ	denotes	is
T9947	29440-29447	JJ	denotes	unknown
T9948	29448-29450	IN	denotes	to
T9949	29451-29455	NN	denotes	date
T9950	29455-29457	,	denotes	,
T9951	29457-29460	CC	denotes	but
T9952	29461-29462	DT	denotes	a
T9953	29463-29467	NN	denotes	role
T9955	29468-29470	IN	denotes	as
T9956	29471-29482	NN	denotes	antioxidant
T9957	29483-29486	VBZ	denotes	has
T9958	29487-29491	VBN	denotes	been
T9954	29492-29500	VBN	denotes	proposed
T9959	29501-29504	IN	denotes	due
T9960	29505-29507	IN	denotes	to
T9961	29508-29511	PRP$	denotes	its
T9962	29512-29519	NN	denotes	ability
T9963	29520-29522	TO	denotes	to
T9964	29523-29527	VB	denotes	bind
T9965	29528-29539	NN	denotes	glutathione
T9966	29540-29541	-LRB-	denotes	(
T9967	29541-29543	CD	denotes	35
T9968	29543-29544	-RRB-	denotes	)
T9969	29544-29545	.	denotes	.
T9970	29545-29715	sentence	denotes	In accordance with this, ectopic expression of mouse Sepw1 renders cells resistant to hydrogen peroxide, and this resistance is dependent on it binding glutathione (36).
T9971	29546-29548	IN	denotes	In
T9973	29549-29559	NN	denotes	accordance
T9974	29560-29564	IN	denotes	with
T9975	29565-29569	DT	denotes	this
T9976	29569-29571	,	denotes	,
T9977	29571-29578	JJ	denotes	ectopic
T9978	29579-29589	NN	denotes	expression
T9979	29590-29592	IN	denotes	of
T9980	29593-29598	NN	denotes	mouse
T9981	29599-29604	NN	denotes	Sepw1
T9972	29605-29612	VBZ	denotes	renders
T9982	29613-29618	NNS	denotes	cells
T9983	29619-29628	JJ	denotes	resistant
T9984	29629-29631	IN	denotes	to
T9985	29632-29640	NN	denotes	hydrogen
T9986	29641-29649	NN	denotes	peroxide
T9987	29649-29651	,	denotes	,
T9988	29651-29654	CC	denotes	and
T9989	29655-29659	DT	denotes	this
T9990	29660-29670	NN	denotes	resistance
T9991	29671-29673	VBZ	denotes	is
T9992	29674-29683	JJ	denotes	dependent
T9993	29684-29686	IN	denotes	on
T9994	29687-29689	PRP	denotes	it
T9996	29690-29697	VBG	denotes	binding
T9995	29698-29709	NN	denotes	glutathione
T9997	29710-29711	-LRB-	denotes	(
T9998	29711-29713	CD	denotes	36
T9999	29713-29714	-RRB-	denotes	)
T10000	29714-29715	.	denotes	.
T10001	29715-29901	sentence	denotes	Furthermore, Amantana et al. (49) showed that expression of a reporter gene fused to a rat Sepw1 promoter fragment can be induced in rat glial cells by copper and zinc, but not cadmium.
T10002	29716-29727	RB	denotes	Furthermore
T10004	29727-29729	,	denotes	,
T10005	29729-29737	NNP	denotes	Amantana
T10006	29738-29740	FW	denotes	et
T10007	29741-29744	FW	denotes	al.
T10008	29745-29746	-LRB-	denotes	(
T10009	29746-29748	CD	denotes	49
T10010	29748-29749	-RRB-	denotes	)
T10003	29750-29756	VBD	denotes	showed
T10011	29757-29761	IN	denotes	that
T10013	29762-29772	NN	denotes	expression
T10014	29773-29775	IN	denotes	of
T10015	29776-29777	DT	denotes	a
T10017	29778-29786	NN	denotes	reporter
T10016	29787-29791	NN	denotes	gene
T10018	29792-29797	VBN	denotes	fused
T10019	29798-29800	IN	denotes	to
T10020	29801-29802	DT	denotes	a
T10022	29803-29806	NN	denotes	rat
T10023	29807-29812	NN	denotes	Sepw1
T10024	29813-29821	NN	denotes	promoter
T10021	29822-29830	NN	denotes	fragment
T10025	29831-29834	MD	denotes	can
T10026	29835-29837	VB	denotes	be
T10012	29838-29845	VBN	denotes	induced
T10027	29846-29848	IN	denotes	in
T10028	29849-29852	NN	denotes	rat
T10030	29853-29858	JJ	denotes	glial
T10029	29859-29864	NNS	denotes	cells
T10031	29865-29867	IN	denotes	by
T10032	29868-29874	NN	denotes	copper
T10033	29875-29878	CC	denotes	and
T10034	29879-29883	NN	denotes	zinc
T10035	29883-29885	,	denotes	,
T10036	29885-29888	CC	denotes	but
T10037	29889-29892	RB	denotes	not
T10038	29893-29900	NN	denotes	cadmium
T10039	29900-29901	.	denotes	.
T10040	29901-30116	sentence	denotes	This response was dependent on an overlapping-inverted MRE sequence located proximal to the rat Sepw1 transcription start (49), even though initial studies failed to demonstrate MTF-1 binding to that sequence (50).
T10041	29902-29906	DT	denotes	This
T10042	29907-29915	NN	denotes	response
T10043	29916-29919	VBD	denotes	was
T10044	29920-29929	JJ	denotes	dependent
T10045	29930-29932	IN	denotes	on
T10046	29933-29935	DT	denotes	an
T10048	29936-29947	JJ	denotes	overlapping
T10050	29947-29948	HYPH	denotes	-
T10049	29948-29956	JJ	denotes	inverted
T10051	29957-29960	NN	denotes	MRE
T10047	29961-29969	NN	denotes	sequence
T10052	29970-29977	VBN	denotes	located
T10053	29978-29986	RB	denotes	proximal
T10054	29987-29989	IN	denotes	to
T10055	29990-29993	DT	denotes	the
T10057	29994-29997	NN	denotes	rat
T10058	29998-30003	NN	denotes	Sepw1
T10059	30004-30017	NN	denotes	transcription
T10056	30018-30023	NN	denotes	start
T10060	30024-30025	-LRB-	denotes	(
T10061	30025-30027	CD	denotes	49
T10062	30027-30028	-RRB-	denotes	)
T10063	30028-30030	,	denotes	,
T10064	30030-30034	RB	denotes	even
T10066	30035-30041	IN	denotes	though
T10067	30042-30049	JJ	denotes	initial
T10068	30050-30057	NNS	denotes	studies
T10065	30058-30064	VBD	denotes	failed
T10069	30065-30067	TO	denotes	to
T10070	30068-30079	VB	denotes	demonstrate
T10071	30080-30083	NN	denotes	MTF
T10073	30083-30084	HYPH	denotes	-
T10074	30084-30085	CD	denotes	1
T10072	30086-30093	NN	denotes	binding
T10075	30094-30096	IN	denotes	to
T10076	30097-30101	DT	denotes	that
T10077	30102-30110	NN	denotes	sequence
T10078	30111-30112	-LRB-	denotes	(
T10079	30112-30114	CD	denotes	50
T10080	30114-30115	-RRB-	denotes	)
T10081	30115-30116	.	denotes	.
T10082	30116-30303	sentence	denotes	Our expression and DNA-binding studies strongly suggest that MTF-1 is important for the basal expression of mouse Sepw1 by binding to the corresponding overlapping-inverted MRE sequence.
T10083	30117-30120	PRP$	denotes	Our
T10084	30121-30131	NN	denotes	expression
T10086	30132-30135	CC	denotes	and
T10087	30136-30139	NN	denotes	DNA
T10089	30139-30140	HYPH	denotes	-
T10088	30140-30147	VBG	denotes	binding
T10090	30148-30155	NNS	denotes	studies
T10091	30156-30164	RB	denotes	strongly
T10085	30165-30172	VBP	denotes	suggest
T10092	30173-30177	IN	denotes	that
T10094	30178-30181	NN	denotes	MTF
T10095	30181-30182	HYPH	denotes	-
T10096	30182-30183	CD	denotes	1
T10093	30184-30186	VBZ	denotes	is
T10097	30187-30196	JJ	denotes	important
T10098	30197-30200	IN	denotes	for
T10099	30201-30204	DT	denotes	the
T10101	30205-30210	JJ	denotes	basal
T10100	30211-30221	NN	denotes	expression
T10102	30222-30224	IN	denotes	of
T10103	30225-30230	NN	denotes	mouse
T10104	30231-30236	NN	denotes	Sepw1
T10105	30237-30239	IN	denotes	by
T10106	30240-30247	VBG	denotes	binding
T10107	30248-30250	IN	denotes	to
T10108	30251-30254	DT	denotes	the
T10110	30255-30268	VBG	denotes	corresponding
T10111	30269-30280	JJ	denotes	overlapping
T10113	30280-30281	HYPH	denotes	-
T10112	30281-30289	JJ	denotes	inverted
T10114	30290-30293	NN	denotes	MRE
T10109	30294-30302	NN	denotes	sequence
T10115	30302-30303	.	denotes	.
T10116	30303-30498	sentence	denotes	Ndrg1, another interesting MTF-1 target gene was named N-myc downstream regulated gene 1 following the discovery that the transcription factor N-myc represses the expression of mouse Ndrg1 (51).
T10117	30304-30309	NN	denotes	Ndrg1
T10119	30309-30311	,	denotes	,
T10120	30311-30318	DT	denotes	another
T10122	30319-30330	JJ	denotes	interesting
T10123	30331-30334	NN	denotes	MTF
T10124	30334-30335	HYPH	denotes	-
T10125	30335-30336	CD	denotes	1
T10126	30337-30343	NN	denotes	target
T10121	30344-30348	NN	denotes	gene
T10127	30349-30352	VBD	denotes	was
T10118	30353-30358	VBN	denotes	named
T10128	30359-30360	NN	denotes	N
T10130	30360-30361	HYPH	denotes	-
T10129	30361-30364	NN	denotes	myc
T10132	30365-30375	RB	denotes	downstream
T10133	30376-30385	VBN	denotes	regulated
T10131	30386-30390	NN	denotes	gene
T10134	30391-30392	CD	denotes	1
T10135	30393-30402	VBG	denotes	following
T10136	30403-30406	DT	denotes	the
T10137	30407-30416	NN	denotes	discovery
T10138	30417-30421	IN	denotes	that
T10140	30422-30425	DT	denotes	the
T10142	30426-30439	NN	denotes	transcription
T10141	30440-30446	NN	denotes	factor
T10143	30447-30448	NN	denotes	N
T10145	30448-30449	HYPH	denotes	-
T10144	30449-30452	NN	denotes	myc
T10139	30453-30462	VBZ	denotes	represses
T10146	30463-30466	DT	denotes	the
T10147	30467-30477	NN	denotes	expression
T10148	30478-30480	IN	denotes	of
T10149	30481-30486	NN	denotes	mouse
T10150	30487-30492	NN	denotes	Ndrg1
T10151	30493-30494	-LRB-	denotes	(
T10152	30494-30496	CD	denotes	51
T10153	30496-30497	-RRB-	denotes	)
T10154	30497-30498	.	denotes	.
T10155	30498-30688	sentence	denotes	Transcription of Ndrg1 and/or its human ortholog is induced by different physiological and cell stress conditions, such as androgens, nickel compounds, DNA damage and hypoxia (37–40,52,53).
T10156	30499-30512	NN	denotes	Transcription
T10158	30513-30515	IN	denotes	of
T10159	30516-30521	NN	denotes	Ndrg1
T10160	30522-30525	CC	denotes	and
T10161	30525-30526	HYPH	denotes	/
T10162	30526-30528	CC	denotes	or
T10163	30529-30532	PRP$	denotes	its
T10165	30533-30538	JJ	denotes	human
T10164	30539-30547	NN	denotes	ortholog
T10166	30548-30550	VBZ	denotes	is
T10157	30551-30558	VBN	denotes	induced
T10167	30559-30561	IN	denotes	by
T10168	30562-30571	JJ	denotes	different
T10170	30572-30585	JJ	denotes	physiological
T10171	30586-30589	CC	denotes	and
T10172	30590-30594	NN	denotes	cell
T10173	30595-30601	NN	denotes	stress
T10169	30602-30612	NNS	denotes	conditions
T10174	30612-30614	,	denotes	,
T10175	30614-30618	JJ	denotes	such
T10176	30619-30621	IN	denotes	as
T10177	30622-30631	NNS	denotes	androgens
T10178	30631-30633	,	denotes	,
T10179	30633-30639	NN	denotes	nickel
T10180	30640-30649	NNS	denotes	compounds
T10181	30649-30651	,	denotes	,
T10182	30651-30654	NN	denotes	DNA
T10183	30655-30661	NN	denotes	damage
T10184	30662-30665	CC	denotes	and
T10185	30666-30673	NN	denotes	hypoxia
T10186	30674-30675	-LRB-	denotes	(
T10188	30675-30677	CD	denotes	37
T10189	30677-30678	SYM	denotes	–
T10190	30678-30680	CD	denotes	40
T10191	30680-30681	,	denotes	,
T10192	30681-30683	CD	denotes	52
T10193	30683-30684	,	denotes	,
T10187	30684-30686	CD	denotes	53
T10194	30686-30687	-RRB-	denotes	)
T10195	30687-30688	.	denotes	.
T10196	30688-30822	sentence	denotes	In addition, the protein is overexpressed in human cancers of many tissues, such as lung, liver, brain, breast, kidney and skin (40).
T10197	30689-30691	IN	denotes	In
T10199	30692-30700	NN	denotes	addition
T10200	30700-30702	,	denotes	,
T10201	30702-30705	DT	denotes	the
T10202	30706-30713	NN	denotes	protein
T10203	30714-30716	VBZ	denotes	is
T10198	30717-30730	VBN	denotes	overexpressed
T10204	30731-30733	IN	denotes	in
T10205	30734-30739	JJ	denotes	human
T10206	30740-30747	NNS	denotes	cancers
T10207	30748-30750	IN	denotes	of
T10208	30751-30755	JJ	denotes	many
T10209	30756-30763	NNS	denotes	tissues
T10210	30763-30765	,	denotes	,
T10211	30765-30769	JJ	denotes	such
T10212	30770-30772	IN	denotes	as
T10213	30773-30777	NN	denotes	lung
T10214	30777-30779	,	denotes	,
T10215	30779-30784	NN	denotes	liver
T10216	30784-30786	,	denotes	,
T10217	30786-30791	NN	denotes	brain
T10218	30791-30793	,	denotes	,
T10219	30793-30799	NN	denotes	breast
T10220	30799-30801	,	denotes	,
T10221	30801-30807	NN	denotes	kidney
T10222	30808-30811	CC	denotes	and
T10223	30812-30816	NN	denotes	skin
T10224	30817-30818	-LRB-	denotes	(
T10225	30818-30820	CD	denotes	40
T10226	30820-30821	-RRB-	denotes	)
T10227	30821-30822	.	denotes	.
T10228	30822-31047	sentence	denotes	Although Ndrg1 and especially its human ortholog have been quite intensely studied, its function remains unclear; however, the induction by stimuli like nickel and hypoxia suggests an involvement in the cell stress response.
T10229	30823-30831	IN	denotes	Although
T10231	30832-30837	NN	denotes	Ndrg1
T10232	30838-30841	CC	denotes	and
T10233	30842-30852	RB	denotes	especially
T10235	30853-30856	PRP$	denotes	its
T10236	30857-30862	JJ	denotes	human
T10234	30863-30871	NN	denotes	ortholog
T10237	30872-30876	VBP	denotes	have
T10238	30877-30881	VBN	denotes	been
T10239	30882-30887	RB	denotes	quite
T10240	30888-30897	RB	denotes	intensely
T10230	30898-30905	VBN	denotes	studied
T10242	30905-30907	,	denotes	,
T10243	30907-30910	PRP$	denotes	its
T10244	30911-30919	NN	denotes	function
T10241	30920-30927	VBZ	denotes	remains
T10246	30928-30935	JJ	denotes	unclear
T10247	30935-30936	:	denotes	;
T10248	30937-30944	RB	denotes	however
T10249	30944-30946	,	denotes	,
T10250	30946-30949	DT	denotes	the
T10251	30950-30959	NN	denotes	induction
T10252	30960-30962	IN	denotes	by
T10253	30963-30970	NNS	denotes	stimuli
T10254	30971-30975	IN	denotes	like
T10255	30976-30982	NN	denotes	nickel
T10256	30983-30986	CC	denotes	and
T10257	30987-30994	NN	denotes	hypoxia
T10245	30995-31003	VBZ	denotes	suggests
T10258	31004-31006	DT	denotes	an
T10259	31007-31018	NN	denotes	involvement
T10260	31019-31021	IN	denotes	in
T10261	31022-31025	DT	denotes	the
T10263	31026-31030	NN	denotes	cell
T10264	31031-31037	NN	denotes	stress
T10262	31038-31046	NN	denotes	response
T10265	31046-31047	.	denotes	.
T10266	31047-31205	sentence	denotes	Such a role is strongly endorsed by our finding that Ndrg1 gene expression is also induced by cadmium, and that MTF-1 plays a crucial role in this induction.
T10267	31048-31052	PDT	denotes	Such
T10269	31053-31054	DT	denotes	a
T10268	31055-31059	NN	denotes	role
T10271	31060-31062	VBZ	denotes	is
T10272	31063-31071	RB	denotes	strongly
T10270	31072-31080	VBN	denotes	endorsed
T10273	31081-31083	IN	denotes	by
T10274	31084-31087	PRP$	denotes	our
T10275	31088-31095	NN	denotes	finding
T10276	31096-31100	IN	denotes	that
T10278	31101-31106	NN	denotes	Ndrg1
T10280	31107-31111	NN	denotes	gene
T10279	31112-31122	NN	denotes	expression
T10281	31123-31125	VBZ	denotes	is
T10282	31126-31130	RB	denotes	also
T10277	31131-31138	VBN	denotes	induced
T10283	31139-31141	IN	denotes	by
T10284	31142-31149	NN	denotes	cadmium
T10285	31149-31151	,	denotes	,
T10286	31151-31154	CC	denotes	and
T10287	31155-31159	IN	denotes	that
T10289	31160-31163	NN	denotes	MTF
T10290	31163-31164	HYPH	denotes	-
T10291	31164-31165	CD	denotes	1
T10288	31166-31171	VBZ	denotes	plays
T10292	31172-31173	DT	denotes	a
T10294	31174-31181	JJ	denotes	crucial
T10293	31182-31186	NN	denotes	role
T10295	31187-31189	IN	denotes	in
T10296	31190-31194	DT	denotes	this
T10297	31195-31204	NN	denotes	induction
T10298	31204-31205	.	denotes	.
T10299	31205-31383	sentence	denotes	In the case of Csrp1, expression analyses and DNA-binding studies indicate that MTF-1 is required for cadmium induction by binding to an MRE upstream of the transcription start.
T10300	31206-31208	IN	denotes	In
T10302	31209-31212	DT	denotes	the
T10303	31213-31217	NN	denotes	case
T10304	31218-31220	IN	denotes	of
T10305	31221-31226	NN	denotes	Csrp1
T10306	31226-31228	,	denotes	,
T10307	31228-31238	NN	denotes	expression
T10308	31239-31247	NNS	denotes	analyses
T10310	31248-31251	CC	denotes	and
T10311	31252-31255	NN	denotes	DNA
T10312	31255-31256	HYPH	denotes	-
T10313	31256-31263	VBG	denotes	binding
T10309	31264-31271	NNS	denotes	studies
T10301	31272-31280	VBP	denotes	indicate
T10314	31281-31285	IN	denotes	that
T10316	31286-31289	NN	denotes	MTF
T10317	31289-31290	HYPH	denotes	-
T10318	31290-31291	CD	denotes	1
T10319	31292-31294	VBZ	denotes	is
T10315	31295-31303	VBN	denotes	required
T10320	31304-31307	IN	denotes	for
T10321	31308-31315	NN	denotes	cadmium
T10322	31316-31325	NN	denotes	induction
T10323	31326-31328	IN	denotes	by
T10324	31329-31336	VBG	denotes	binding
T10325	31337-31339	IN	denotes	to
T10326	31340-31342	DT	denotes	an
T10327	31343-31346	NN	denotes	MRE
T10328	31347-31355	RB	denotes	upstream
T10329	31356-31358	IN	denotes	of
T10330	31359-31362	DT	denotes	the
T10332	31363-31376	NN	denotes	transcription
T10331	31377-31382	NN	denotes	start
T10333	31382-31383	.	denotes	.
T10334	31383-31652	sentence	denotes	Studies with human, avian and chicken CSRP1 have shown that this protein is localized at adhesion plaques and in association with filamentous actin, and interacts with the adhesion plaque protein zyxin, as well as the actin-cross-linking protein alpha-actinin (54–57).
T10335	31384-31391	NNS	denotes	Studies
T10337	31392-31396	IN	denotes	with
T10338	31397-31402	JJ	denotes	human
T10340	31402-31404	,	denotes	,
T10341	31404-31409	JJ	denotes	avian
T10342	31410-31413	CC	denotes	and
T10343	31414-31421	NN	denotes	chicken
T10339	31422-31427	NN	denotes	CSRP1
T10344	31428-31432	VBP	denotes	have
T10336	31433-31438	VBN	denotes	shown
T10345	31439-31443	IN	denotes	that
T10347	31444-31448	DT	denotes	this
T10348	31449-31456	NN	denotes	protein
T10349	31457-31459	VBZ	denotes	is
T10346	31460-31469	VBN	denotes	localized
T10350	31470-31472	IN	denotes	at
T10351	31473-31481	NN	denotes	adhesion
T10352	31482-31489	NNS	denotes	plaques
T10353	31490-31493	CC	denotes	and
T10354	31494-31496	IN	denotes	in
T10355	31497-31508	NN	denotes	association
T10356	31509-31513	IN	denotes	with
T10357	31514-31525	JJ	denotes	filamentous
T10358	31526-31531	NN	denotes	actin
T10359	31531-31533	,	denotes	,
T10360	31533-31536	CC	denotes	and
T10361	31537-31546	VBZ	denotes	interacts
T10362	31547-31551	IN	denotes	with
T10363	31552-31555	DT	denotes	the
T10365	31556-31564	NN	denotes	adhesion
T10366	31565-31571	NN	denotes	plaque
T10364	31572-31579	NN	denotes	protein
T10367	31580-31585	NN	denotes	zyxin
T10368	31585-31587	,	denotes	,
T10369	31587-31589	RB	denotes	as
T10371	31590-31594	RB	denotes	well
T10370	31595-31597	IN	denotes	as
T10372	31598-31601	DT	denotes	the
T10374	31602-31607	NN	denotes	actin
T10376	31607-31608	HYPH	denotes	-
T10375	31608-31621	VBG	denotes	cross-linking
T10373	31622-31629	NN	denotes	protein
T10377	31630-31635	NN	denotes	alpha
T10379	31635-31636	HYPH	denotes	-
T10378	31636-31643	NN	denotes	actinin
T10380	31644-31645	-LRB-	denotes	(
T10381	31645-31647	CD	denotes	54
T10382	31647-31648	SYM	denotes	–
T10383	31648-31650	CD	denotes	57
T10384	31650-31651	-RRB-	denotes	)
T10385	31651-31652	.	denotes	.
T10386	31652-31738	sentence	denotes	The ability to bind these partners suggests a role in cytoskeletal organization (58).
T10387	31653-31656	DT	denotes	The
T10388	31657-31664	NN	denotes	ability
T10390	31665-31667	TO	denotes	to
T10391	31668-31672	VB	denotes	bind
T10392	31673-31678	DT	denotes	these
T10393	31679-31687	NNS	denotes	partners
T10389	31688-31696	VBZ	denotes	suggests
T10394	31697-31698	DT	denotes	a
T10395	31699-31703	NN	denotes	role
T10396	31704-31706	IN	denotes	in
T10397	31707-31719	JJ	denotes	cytoskeletal
T10398	31720-31732	NN	denotes	organization
T10399	31733-31734	-LRB-	denotes	(
T10400	31734-31736	CD	denotes	58
T10401	31736-31737	-RRB-	denotes	)
T10402	31737-31738	.	denotes	.
T10403	31738-31877	sentence	denotes	Exposure of cultured cells to cadmium causes a decrease in, and destruction of, cellular contact proteins and the actin cytoskeleton (59).
T10404	31739-31747	NN	denotes	Exposure
T10406	31748-31750	IN	denotes	of
T10407	31751-31759	VBN	denotes	cultured
T10408	31760-31765	NNS	denotes	cells
T10409	31766-31768	IN	denotes	to
T10410	31769-31776	NN	denotes	cadmium
T10405	31777-31783	VBZ	denotes	causes
T10411	31784-31785	DT	denotes	a
T10412	31786-31794	NN	denotes	decrease
T10413	31795-31797	IN	denotes	in
T10414	31797-31799	,	denotes	,
T10415	31799-31802	CC	denotes	and
T10416	31803-31814	NN	denotes	destruction
T10417	31815-31817	IN	denotes	of
T10418	31817-31819	,	denotes	,
T10419	31819-31827	JJ	denotes	cellular
T10420	31828-31835	NN	denotes	contact
T10421	31836-31844	NN	denotes	proteins
T10422	31845-31848	CC	denotes	and
T10423	31849-31852	DT	denotes	the
T10425	31853-31858	NN	denotes	actin
T10424	31859-31871	NN	denotes	cytoskeleton
T10426	31872-31873	-LRB-	denotes	(
T10427	31873-31875	CD	denotes	59
T10428	31875-31876	-RRB-	denotes	)
T10429	31876-31877	.	denotes	.
T10430	31877-32093	sentence	denotes	In the proximal tubule cells of the rat kidney, a partial loss of actin and the actin-bundling protein villin is observed upon cadmium treatment, as well as the derangement and depolymerization of microtubules (60).
T10431	31878-31880	IN	denotes	In
T10433	31881-31884	DT	denotes	the
T10435	31885-31893	JJ	denotes	proximal
T10436	31894-31900	NN	denotes	tubule
T10434	31901-31906	NNS	denotes	cells
T10437	31907-31909	IN	denotes	of
T10438	31910-31913	DT	denotes	the
T10440	31914-31917	NN	denotes	rat
T10439	31918-31924	NN	denotes	kidney
T10441	31924-31926	,	denotes	,
T10442	31926-31927	DT	denotes	a
T10444	31928-31935	JJ	denotes	partial
T10443	31936-31940	NN	denotes	loss
T10445	31941-31943	IN	denotes	of
T10446	31944-31949	NN	denotes	actin
T10447	31950-31953	CC	denotes	and
T10448	31954-31957	DT	denotes	the
T10450	31958-31963	NN	denotes	actin
T10452	31963-31964	HYPH	denotes	-
T10451	31964-31972	VBG	denotes	bundling
T10449	31973-31980	NN	denotes	protein
T10453	31981-31987	NN	denotes	villin
T10454	31988-31990	VBZ	denotes	is
T10432	31991-31999	VBN	denotes	observed
T10455	32000-32004	IN	denotes	upon
T10456	32005-32012	NN	denotes	cadmium
T10457	32013-32022	NN	denotes	treatment
T10458	32022-32024	,	denotes	,
T10459	32024-32026	RB	denotes	as
T10461	32027-32031	RB	denotes	well
T10460	32032-32034	IN	denotes	as
T10462	32035-32038	DT	denotes	the
T10463	32039-32050	NN	denotes	derangement
T10464	32051-32054	CC	denotes	and
T10465	32055-32071	NN	denotes	depolymerization
T10466	32072-32074	IN	denotes	of
T10467	32075-32087	NNS	denotes	microtubules
T10468	32088-32089	-LRB-	denotes	(
T10469	32089-32091	CD	denotes	60
T10470	32091-32092	-RRB-	denotes	)
T10471	32092-32093	.	denotes	.
T10472	32093-32274	sentence	denotes	Assuming that CSRP1 is important for the organization of cytoskeletal elements in the mouse, its upregulation by cadmium might protect the organism from damage of the cytoskeleton.
T10473	32094-32102	VBG	denotes	Assuming
T10475	32103-32107	IN	denotes	that
T10477	32108-32113	NN	denotes	CSRP1
T10476	32114-32116	VBZ	denotes	is
T10478	32117-32126	JJ	denotes	important
T10479	32127-32130	IN	denotes	for
T10480	32131-32134	DT	denotes	the
T10481	32135-32147	NN	denotes	organization
T10482	32148-32150	IN	denotes	of
T10483	32151-32163	JJ	denotes	cytoskeletal
T10484	32164-32172	NNS	denotes	elements
T10485	32173-32175	IN	denotes	in
T10486	32176-32179	DT	denotes	the
T10487	32180-32185	NN	denotes	mouse
T10488	32185-32187	,	denotes	,
T10489	32187-32190	PRP$	denotes	its
T10490	32191-32203	NN	denotes	upregulation
T10491	32204-32206	IN	denotes	by
T10492	32207-32214	NN	denotes	cadmium
T10493	32215-32220	MD	denotes	might
T10474	32221-32228	VB	denotes	protect
T10494	32229-32232	DT	denotes	the
T10495	32233-32241	NN	denotes	organism
T10496	32242-32246	IN	denotes	from
T10497	32247-32253	NN	denotes	damage
T10498	32254-32256	IN	denotes	of
T10499	32257-32260	DT	denotes	the
T10500	32261-32273	NN	denotes	cytoskeleton
T10501	32273-32274	.	denotes	.
T10502	32274-32342	sentence	denotes	Such a mechanism would expand the role of MTF-1 in stress response.
T10503	32275-32279	PDT	denotes	Such
T10505	32280-32281	DT	denotes	a
T10504	32282-32291	NN	denotes	mechanism
T10507	32292-32297	MD	denotes	would
T10506	32298-32304	VB	denotes	expand
T10508	32305-32308	DT	denotes	the
T10509	32309-32313	NN	denotes	role
T10510	32314-32316	IN	denotes	of
T10511	32317-32320	NN	denotes	MTF
T10512	32320-32321	HYPH	denotes	-
T10513	32321-32322	CD	denotes	1
T10514	32323-32325	IN	denotes	in
T10515	32326-32332	NN	denotes	stress
T10516	32333-32341	NN	denotes	response
T10517	32341-32342	.	denotes	.
T10518	32342-32542	sentence	denotes	Our expression studies also suggest that MTF-1 represses basal transcription of Slc39a10, in contrast to its role as activator for the expression of other target genes like Mt1, Mt2, and Znt1 (4,19).
T10519	32343-32346	PRP$	denotes	Our
T10521	32347-32357	NN	denotes	expression
T10520	32358-32365	NNS	denotes	studies
T10523	32366-32370	RB	denotes	also
T10522	32371-32378	VBP	denotes	suggest
T10524	32379-32383	IN	denotes	that
T10526	32384-32387	NN	denotes	MTF
T10527	32387-32388	HYPH	denotes	-
T10528	32388-32389	CD	denotes	1
T10525	32390-32399	VBZ	denotes	represses
T10529	32400-32405	JJ	denotes	basal
T10530	32406-32419	NN	denotes	transcription
T10531	32420-32422	IN	denotes	of
T10532	32423-32431	NN	denotes	Slc39a10
T10533	32431-32433	,	denotes	,
T10534	32433-32435	IN	denotes	in
T10535	32436-32444	NN	denotes	contrast
T10536	32445-32447	IN	denotes	to
T10537	32448-32451	PRP$	denotes	its
T10538	32452-32456	NN	denotes	role
T10539	32457-32459	IN	denotes	as
T10540	32460-32469	NN	denotes	activator
T10541	32470-32473	IN	denotes	for
T10542	32474-32477	DT	denotes	the
T10543	32478-32488	NN	denotes	expression
T10544	32489-32491	IN	denotes	of
T10545	32492-32497	JJ	denotes	other
T10547	32498-32504	NN	denotes	target
T10546	32505-32510	NNS	denotes	genes
T10548	32511-32515	IN	denotes	like
T10549	32516-32519	NN	denotes	Mt1
T10550	32519-32521	,	denotes	,
T10551	32521-32524	NN	denotes	Mt2
T10552	32524-32526	,	denotes	,
T10553	32526-32529	CC	denotes	and
T10554	32530-32534	NN	denotes	Znt1
T10555	32535-32536	-LRB-	denotes	(
T10557	32536-32537	CD	denotes	4
T10558	32537-32538	,	denotes	,
T10556	32538-32540	CD	denotes	19
T10559	32540-32541	-RRB-	denotes	)
T10560	32541-32542	.	denotes	.
T10561	32542-32651	sentence	denotes	SLC39A10 is one of 14 mouse SLC39 members, which belong to the ZIP family of metal ion transporters (43,44).
T10562	32543-32551	NN	denotes	SLC39A10
T10563	32552-32554	VBZ	denotes	is
T10564	32555-32558	CD	denotes	one
T10565	32559-32561	IN	denotes	of
T10566	32562-32564	CD	denotes	14
T10568	32565-32570	NN	denotes	mouse
T10569	32571-32576	NN	denotes	SLC39
T10567	32577-32584	NNS	denotes	members
T10570	32584-32586	,	denotes	,
T10571	32586-32591	WDT	denotes	which
T10572	32592-32598	VBP	denotes	belong
T10573	32599-32601	IN	denotes	to
T10574	32602-32605	DT	denotes	the
T10576	32606-32609	NN	denotes	ZIP
T10575	32610-32616	NN	denotes	family
T10577	32617-32619	IN	denotes	of
T10578	32620-32625	NN	denotes	metal
T10579	32626-32629	NN	denotes	ion
T10580	32630-32642	NNS	denotes	transporters
T10581	32643-32644	-LRB-	denotes	(
T10583	32644-32646	CD	denotes	43
T10584	32646-32647	,	denotes	,
T10582	32647-32649	CD	denotes	44
T10585	32649-32650	-RRB-	denotes	)
T10586	32650-32651	.	denotes	.
T10587	32651-32837	sentence	denotes	All members of the ZIP family characterized so far increase intracellular cytoplasmic metal ion concentrations by promoting extracellular and vesicular ion transport into the cytoplasm.
T10588	32652-32655	DT	denotes	All
T10589	32656-32663	NNS	denotes	members
T10591	32664-32666	IN	denotes	of
T10592	32667-32670	DT	denotes	the
T10594	32671-32674	NN	denotes	ZIP
T10593	32675-32681	NN	denotes	family
T10595	32682-32695	VBN	denotes	characterized
T10596	32696-32698	RB	denotes	so
T10597	32699-32702	RB	denotes	far
T10590	32703-32711	VBP	denotes	increase
T10598	32712-32725	JJ	denotes	intracellular
T10600	32726-32737	JJ	denotes	cytoplasmic
T10601	32738-32743	NN	denotes	metal
T10602	32744-32747	NN	denotes	ion
T10599	32748-32762	NNS	denotes	concentrations
T10603	32763-32765	IN	denotes	by
T10604	32766-32775	VBG	denotes	promoting
T10605	32776-32789	JJ	denotes	extracellular
T10607	32790-32793	CC	denotes	and
T10608	32794-32803	JJ	denotes	vesicular
T10609	32804-32807	NN	denotes	ion
T10606	32808-32817	NN	denotes	transport
T10610	32818-32822	IN	denotes	into
T10611	32823-32826	DT	denotes	the
T10612	32827-32836	NN	denotes	cytoplasm
T10613	32836-32837	.	denotes	.
T10614	32837-32940	sentence	denotes	ZIP proteins have been reported to be transporters of zinc, iron, manganese and/or cadmium (44,61–63).
T10615	32838-32841	NN	denotes	ZIP
T10616	32842-32850	NN	denotes	proteins
T10618	32851-32855	VBP	denotes	have
T10619	32856-32860	VBN	denotes	been
T10617	32861-32869	VBN	denotes	reported
T10620	32870-32872	TO	denotes	to
T10621	32873-32875	VB	denotes	be
T10622	32876-32888	NNS	denotes	transporters
T10623	32889-32891	IN	denotes	of
T10624	32892-32896	NN	denotes	zinc
T10625	32896-32898	,	denotes	,
T10626	32898-32902	NN	denotes	iron
T10627	32902-32904	,	denotes	,
T10628	32904-32913	NN	denotes	manganese
T10629	32914-32917	CC	denotes	and
T10630	32917-32918	HYPH	denotes	/
T10631	32918-32920	CC	denotes	or
T10632	32921-32928	NN	denotes	cadmium
T10633	32929-32930	-LRB-	denotes	(
T10634	32930-32932	CD	denotes	44
T10635	32932-32933	,	denotes	,
T10636	32933-32935	CD	denotes	61
T10637	32935-32936	SYM	denotes	–
T10638	32936-32938	CD	denotes	63
T10639	32938-32939	-RRB-	denotes	)
T10640	32939-32940	.	denotes	.
T10641	32940-33060	sentence	denotes	Although SLC39A10 is largely uncharacterized (44), it is referred to in several databases as putative zinc transporter.
T10642	32941-32949	IN	denotes	Although
T10644	32950-32958	NN	denotes	SLC39A10
T10643	32959-32961	VBZ	denotes	is
T10646	32962-32969	RB	denotes	largely
T10647	32970-32985	JJ	denotes	uncharacterized
T10648	32986-32987	-LRB-	denotes	(
T10649	32987-32989	CD	denotes	44
T10650	32989-32990	-RRB-	denotes	)
T10651	32990-32992	,	denotes	,
T10652	32992-32994	PRP	denotes	it
T10653	32995-32997	VBZ	denotes	is
T10645	32998-33006	VBN	denotes	referred
T10654	33007-33009	IN	denotes	to
T10655	33010-33012	IN	denotes	in
T10656	33013-33020	JJ	denotes	several
T10657	33021-33030	NNS	denotes	databases
T10658	33031-33033	IN	denotes	as
T10659	33034-33042	JJ	denotes	putative
T10661	33043-33047	NN	denotes	zinc
T10660	33048-33059	NN	denotes	transporter
T10662	33059-33060	.	denotes	.
T10663	33060-33188	sentence	denotes	It has been previously shown that MTF-1 is important for both basal expression and metal induction of the mouse Znt1 gene (19).
T10664	33061-33063	PRP	denotes	It
T10666	33064-33067	VBZ	denotes	has
T10667	33068-33072	VBN	denotes	been
T10668	33073-33083	RB	denotes	previously
T10665	33084-33089	VBN	denotes	shown
T10669	33090-33094	IN	denotes	that
T10671	33095-33098	NN	denotes	MTF
T10672	33098-33099	HYPH	denotes	-
T10673	33099-33100	CD	denotes	1
T10670	33101-33103	VBZ	denotes	is
T10674	33104-33113	JJ	denotes	important
T10675	33114-33117	IN	denotes	for
T10676	33118-33122	CC	denotes	both
T10678	33123-33128	JJ	denotes	basal
T10677	33129-33139	NN	denotes	expression
T10679	33140-33143	CC	denotes	and
T10680	33144-33149	NN	denotes	metal
T10681	33150-33159	NN	denotes	induction
T10682	33160-33162	IN	denotes	of
T10683	33163-33166	DT	denotes	the
T10685	33167-33172	NN	denotes	mouse
T10686	33173-33177	NN	denotes	Znt1
T10684	33178-33182	NN	denotes	gene
T10687	33183-33184	-LRB-	denotes	(
T10688	33184-33186	CD	denotes	19
T10689	33186-33187	-RRB-	denotes	)
T10690	33187-33188	.	denotes	.
T10691	33188-33357	sentence	denotes	ZnT proteins represent a different family of transporters that reduce intracellular cytoplasmic zinc by promoting zinc efflux from cells or into intracellular vesicles.
T10692	33189-33192	NN	denotes	ZnT
T10693	33193-33201	NN	denotes	proteins
T10694	33202-33211	VBP	denotes	represent
T10695	33212-33213	DT	denotes	a
T10697	33214-33223	JJ	denotes	different
T10696	33224-33230	NN	denotes	family
T10698	33231-33233	IN	denotes	of
T10699	33234-33246	NNS	denotes	transporters
T10700	33247-33251	WDT	denotes	that
T10701	33252-33258	VBP	denotes	reduce
T10702	33259-33272	JJ	denotes	intracellular
T10704	33273-33284	JJ	denotes	cytoplasmic
T10703	33285-33289	NN	denotes	zinc
T10705	33290-33292	IN	denotes	by
T10706	33293-33302	VBG	denotes	promoting
T10707	33303-33307	NN	denotes	zinc
T10708	33308-33314	NN	denotes	efflux
T10709	33315-33319	IN	denotes	from
T10710	33320-33325	NNS	denotes	cells
T10711	33326-33328	CC	denotes	or
T10712	33329-33333	IN	denotes	into
T10713	33334-33347	JJ	denotes	intracellular
T10714	33348-33356	NNS	denotes	vesicles
T10715	33356-33357	.	denotes	.
T10716	33357-33487	sentence	denotes	Thus, members of the ZnT and ZIP family with zinc as predominant substrate have opposite roles in cellular zinc homeostasis (43).
T10717	33358-33362	RB	denotes	Thus
T10719	33362-33364	,	denotes	,
T10720	33364-33371	NNS	denotes	members
T10721	33372-33374	IN	denotes	of
T10722	33375-33378	DT	denotes	the
T10724	33379-33382	NN	denotes	ZnT
T10725	33383-33386	CC	denotes	and
T10726	33387-33390	NN	denotes	ZIP
T10723	33391-33397	NN	denotes	family
T10727	33398-33402	IN	denotes	with
T10728	33403-33407	NN	denotes	zinc
T10729	33408-33410	IN	denotes	as
T10730	33411-33422	JJ	denotes	predominant
T10731	33423-33432	NN	denotes	substrate
T10718	33433-33437	VBP	denotes	have
T10732	33438-33446	JJ	denotes	opposite
T10733	33447-33452	NNS	denotes	roles
T10734	33453-33455	IN	denotes	in
T10735	33456-33464	JJ	denotes	cellular
T10737	33465-33469	NN	denotes	zinc
T10736	33470-33481	NN	denotes	homeostasis
T10738	33482-33483	-LRB-	denotes	(
T10739	33483-33485	CD	denotes	43
T10740	33485-33486	-RRB-	denotes	)
T10741	33486-33487	.	denotes	.
T10742	33487-33652	sentence	denotes	Assuming that SLC39A10 is indeed a zinc transporter, MTF-1 would control expression of two zinc transporters with antagonistic functions, namely, Znt1 and Slc39a10.
T10743	33488-33496	VBG	denotes	Assuming
T10745	33497-33501	IN	denotes	that
T10747	33502-33510	NN	denotes	SLC39A10
T10746	33511-33513	VBZ	denotes	is
T10748	33514-33520	RB	denotes	indeed
T10749	33521-33522	DT	denotes	a
T10751	33523-33527	NN	denotes	zinc
T10750	33528-33539	NN	denotes	transporter
T10752	33539-33541	,	denotes	,
T10753	33541-33544	NN	denotes	MTF
T10754	33544-33545	HYPH	denotes	-
T10755	33545-33546	CD	denotes	1
T10756	33547-33552	MD	denotes	would
T10744	33553-33560	VB	denotes	control
T10757	33561-33571	NN	denotes	expression
T10758	33572-33574	IN	denotes	of
T10759	33575-33578	CD	denotes	two
T10761	33579-33583	NN	denotes	zinc
T10760	33584-33596	NNS	denotes	transporters
T10762	33597-33601	IN	denotes	with
T10763	33602-33614	JJ	denotes	antagonistic
T10764	33615-33624	NNS	denotes	functions
T10765	33624-33626	,	denotes	,
T10766	33626-33632	RB	denotes	namely
T10768	33632-33634	,	denotes	,
T10767	33634-33638	NN	denotes	Znt1
T10769	33639-33642	CC	denotes	and
T10770	33643-33651	NN	denotes	Slc39a10
T10771	33651-33652	.	denotes	.
T10772	33652-33763	sentence	denotes	Specific binding of MTF-1 was observed for an MRE located just downstream of the Slc39a10 transcription start.
T10773	33653-33661	JJ	denotes	Specific
T10774	33662-33669	NN	denotes	binding
T10776	33670-33672	IN	denotes	of
T10777	33673-33676	NN	denotes	MTF
T10778	33676-33677	HYPH	denotes	-
T10779	33677-33678	CD	denotes	1
T10780	33679-33682	VBD	denotes	was
T10775	33683-33691	VBN	denotes	observed
T10781	33692-33695	IN	denotes	for
T10782	33696-33698	DT	denotes	an
T10783	33699-33702	NN	denotes	MRE
T10784	33703-33710	VBN	denotes	located
T10785	33711-33715	RB	denotes	just
T10786	33716-33726	RB	denotes	downstream
T10787	33727-33729	IN	denotes	of
T10788	33730-33733	DT	denotes	the
T10790	33734-33742	NN	denotes	Slc39a10
T10791	33743-33756	NN	denotes	transcription
T10789	33757-33762	NN	denotes	start
T10792	33762-33763	.	denotes	.
T10793	33763-33984	sentence	denotes	In a simple model, such a binding could interfere with the accessibility of the transcriptional start site for RNA polymerase II and/or general transcription factors, thus preventing transcription initiation of the gene.
T10794	33764-33766	IN	denotes	In
T10796	33767-33768	DT	denotes	a
T10798	33769-33775	JJ	denotes	simple
T10797	33776-33781	NN	denotes	model
T10799	33781-33783	,	denotes	,
T10800	33783-33787	PDT	denotes	such
T10802	33788-33789	DT	denotes	a
T10801	33790-33797	NN	denotes	binding
T10803	33798-33803	MD	denotes	could
T10795	33804-33813	VB	denotes	interfere
T10804	33814-33818	IN	denotes	with
T10805	33819-33822	DT	denotes	the
T10806	33823-33836	NN	denotes	accessibility
T10807	33837-33839	IN	denotes	of
T10808	33840-33843	DT	denotes	the
T10810	33844-33859	JJ	denotes	transcriptional
T10811	33860-33865	NN	denotes	start
T10809	33866-33870	NN	denotes	site
T10812	33871-33874	IN	denotes	for
T10813	33875-33878	NN	denotes	RNA
T10814	33879-33889	NN	denotes	polymerase
T10815	33890-33892	CD	denotes	II
T10816	33893-33896	CC	denotes	and
T10817	33896-33897	HYPH	denotes	/
T10818	33897-33899	CC	denotes	or
T10819	33900-33907	JJ	denotes	general
T10821	33908-33921	NN	denotes	transcription
T10820	33922-33929	NNS	denotes	factors
T10822	33929-33931	,	denotes	,
T10823	33931-33935	RB	denotes	thus
T10824	33936-33946	VBG	denotes	preventing
T10825	33947-33960	NN	denotes	transcription
T10826	33961-33971	NN	denotes	initiation
T10827	33972-33974	IN	denotes	of
T10828	33975-33978	DT	denotes	the
T10829	33979-33983	NN	denotes	gene
T10830	33983-33984	.	denotes	.
T10831	33984-34151	sentence	denotes	Indeed, such a mechanism has been described in yeast for the zinc-responsive activator protein 1 (Zap1) and its target gene, zinc-regulated transporter 2 (ZRT2) (64).
T10832	33985-33991	RB	denotes	Indeed
T10834	33991-33993	,	denotes	,
T10835	33993-33997	PDT	denotes	such
T10837	33998-33999	DT	denotes	a
T10836	34000-34009	NN	denotes	mechanism
T10838	34010-34013	VBZ	denotes	has
T10839	34014-34018	VBN	denotes	been
T10833	34019-34028	VBN	denotes	described
T10840	34029-34031	IN	denotes	in
T10841	34032-34037	NN	denotes	yeast
T10842	34038-34041	IN	denotes	for
T10843	34042-34045	DT	denotes	the
T10845	34046-34050	NN	denotes	zinc
T10847	34050-34051	HYPH	denotes	-
T10846	34051-34061	JJ	denotes	responsive
T10848	34062-34071	NN	denotes	activator
T10844	34072-34079	NN	denotes	protein
T10849	34080-34081	CD	denotes	1
T10850	34082-34083	-LRB-	denotes	(
T10851	34083-34087	NN	denotes	Zap1
T10852	34087-34088	-RRB-	denotes	)
T10853	34089-34092	CC	denotes	and
T10854	34093-34096	PRP$	denotes	its
T10856	34097-34103	NN	denotes	target
T10855	34104-34108	NN	denotes	gene
T10857	34108-34110	,	denotes	,
T10858	34110-34114	NN	denotes	zinc
T10860	34114-34115	HYPH	denotes	-
T10861	34115-34124	VBN	denotes	regulated
T10859	34125-34136	NN	denotes	transporter
T10862	34137-34138	CD	denotes	2
T10863	34139-34140	-LRB-	denotes	(
T10864	34140-34144	NN	denotes	ZRT2
T10865	34144-34145	-RRB-	denotes	)
T10866	34146-34147	-LRB-	denotes	(
T10867	34147-34149	CD	denotes	64
T10868	34149-34150	-RRB-	denotes	)
T10869	34150-34151	.	denotes	.
T10870	34151-34273	sentence	denotes	However, the inhibition of Slc39a10 expression by MTF-1 may well be more complex than a competition for promoter binding.
T10871	34152-34159	RB	denotes	However
T10873	34159-34161	,	denotes	,
T10874	34161-34164	DT	denotes	the
T10875	34165-34175	NN	denotes	inhibition
T10876	34176-34178	IN	denotes	of
T10877	34179-34187	NN	denotes	Slc39a10
T10878	34188-34198	NN	denotes	expression
T10879	34199-34201	IN	denotes	by
T10880	34202-34205	NN	denotes	MTF
T10881	34205-34206	HYPH	denotes	-
T10882	34206-34207	CD	denotes	1
T10883	34208-34211	MD	denotes	may
T10884	34212-34216	RB	denotes	well
T10872	34217-34219	VB	denotes	be
T10885	34220-34224	RBR	denotes	more
T10886	34225-34232	JJ	denotes	complex
T10887	34233-34237	IN	denotes	than
T10888	34238-34239	DT	denotes	a
T10889	34240-34251	NN	denotes	competition
T10890	34252-34255	IN	denotes	for
T10891	34256-34264	NN	denotes	promoter
T10892	34265-34272	NN	denotes	binding
T10893	34272-34273	.	denotes	.
T10894	34273-34429	sentence	denotes	Independent of MTF-1, cadmium treatment also leads to downregulation of Slc39a10 transcripts, suggesting that some other factor is mediating this response.
T10895	34274-34285	JJ	denotes	Independent
T10897	34286-34288	IN	denotes	of
T10898	34289-34292	NN	denotes	MTF
T10899	34292-34293	HYPH	denotes	-
T10900	34293-34294	CD	denotes	1
T10901	34294-34296	,	denotes	,
T10902	34296-34303	NN	denotes	cadmium
T10903	34304-34313	NN	denotes	treatment
T10904	34314-34318	RB	denotes	also
T10896	34319-34324	VBZ	denotes	leads
T10905	34325-34327	IN	denotes	to
T10906	34328-34342	NN	denotes	downregulation
T10907	34343-34345	IN	denotes	of
T10908	34346-34354	NN	denotes	Slc39a10
T10909	34355-34366	NNS	denotes	transcripts
T10910	34366-34368	,	denotes	,
T10911	34368-34378	VBG	denotes	suggesting
T10912	34379-34383	IN	denotes	that
T10914	34384-34388	DT	denotes	some
T10916	34389-34394	JJ	denotes	other
T10915	34395-34401	NN	denotes	factor
T10917	34402-34404	VBZ	denotes	is
T10913	34405-34414	VBG	denotes	mediating
T10918	34415-34419	DT	denotes	this
T10919	34420-34428	NN	denotes	response
T10920	34428-34429	.	denotes	.
T10921	34429-34728	sentence	denotes	A previous target gene search for MTF-1 with mouse embryos of conventional Mtf1 knockout phenotype revealed, besides metallothionein genes, the multifunctional alpha-fetoprotein (Afp) and the liver-enriched transcription factor CCAAT/enhancer binding protein alpha (Cebpa) as prime candidates (65).
T10922	34430-34431	DT	denotes	A
T10924	34432-34440	JJ	denotes	previous
T10925	34441-34447	NN	denotes	target
T10926	34448-34452	NN	denotes	gene
T10923	34453-34459	NN	denotes	search
T10928	34460-34463	IN	denotes	for
T10929	34464-34467	NN	denotes	MTF
T10930	34467-34468	HYPH	denotes	-
T10931	34468-34469	CD	denotes	1
T10932	34470-34474	IN	denotes	with
T10933	34475-34480	NN	denotes	mouse
T10934	34481-34488	NNS	denotes	embryos
T10935	34489-34491	IN	denotes	of
T10936	34492-34504	JJ	denotes	conventional
T10938	34505-34509	NN	denotes	Mtf1
T10939	34510-34518	NN	denotes	knockout
T10937	34519-34528	NN	denotes	phenotype
T10927	34529-34537	VBD	denotes	revealed
T10940	34537-34539	,	denotes	,
T10941	34539-34546	IN	denotes	besides
T10942	34547-34562	NN	denotes	metallothionein
T10943	34563-34568	NNS	denotes	genes
T10944	34568-34570	,	denotes	,
T10945	34570-34573	DT	denotes	the
T10947	34574-34589	JJ	denotes	multifunctional
T10948	34590-34595	NN	denotes	alpha
T10949	34595-34596	HYPH	denotes	-
T10946	34596-34607	NN	denotes	fetoprotein
T10950	34608-34609	-LRB-	denotes	(
T10951	34609-34612	NN	denotes	Afp
T10952	34612-34613	-RRB-	denotes	)
T10953	34614-34617	CC	denotes	and
T10954	34618-34621	DT	denotes	the
T10956	34622-34627	NN	denotes	liver
T10958	34627-34628	HYPH	denotes	-
T10957	34628-34636	VBN	denotes	enriched
T10959	34637-34650	NN	denotes	transcription
T10955	34651-34657	NN	denotes	factor
T10960	34658-34663	NN	denotes	CCAAT
T10962	34663-34664	HYPH	denotes	/
T10963	34664-34672	NN	denotes	enhancer
T10964	34673-34680	NN	denotes	binding
T10961	34681-34688	NN	denotes	protein
T10965	34689-34694	SYM	denotes	alpha
T10966	34695-34696	-LRB-	denotes	(
T10967	34696-34701	NN	denotes	Cebpa
T10968	34701-34702	-RRB-	denotes	)
T10969	34703-34705	IN	denotes	as
T10970	34706-34711	JJ	denotes	prime
T10971	34712-34722	NNS	denotes	candidates
T10972	34723-34724	-LRB-	denotes	(
T10973	34724-34726	CD	denotes	65
T10974	34726-34727	-RRB-	denotes	)
T10975	34727-34728	.	denotes	.
T10976	34728-34841	sentence	denotes	After an early onset during hepatogenesis, Afp expression is repressed postnatally and replaced by albumin (66).
T10977	34729-34734	IN	denotes	After
T10979	34735-34737	DT	denotes	an
T10981	34738-34743	JJ	denotes	early
T10980	34744-34749	NN	denotes	onset
T10982	34750-34756	IN	denotes	during
T10983	34757-34770	NN	denotes	hepatogenesis
T10984	34770-34772	,	denotes	,
T10985	34772-34775	NN	denotes	Afp
T10986	34776-34786	NN	denotes	expression
T10987	34787-34789	VBZ	denotes	is
T10978	34790-34799	VBN	denotes	repressed
T10988	34800-34811	RB	denotes	postnatally
T10989	34812-34815	CC	denotes	and
T10990	34816-34824	VBN	denotes	replaced
T10991	34825-34827	IN	denotes	by
T10992	34828-34835	NN	denotes	albumin
T10993	34836-34837	-LRB-	denotes	(
T10994	34837-34839	CD	denotes	66
T10995	34839-34840	-RRB-	denotes	)
T10996	34840-34841	.	denotes	.
T10997	34841-34932	sentence	denotes	Thus, our adult Mtf1Mx-cre mice lacking MTF-1 were not suitable to analyze Afp expression.
T10998	34842-34846	RB	denotes	Thus
T11000	34846-34848	,	denotes	,
T11001	34848-34851	PRP$	denotes	our
T11003	34852-34857	JJ	denotes	adult
T11004	34858-34864	NN	denotes	Mtf1Mx
T11006	34864-34865	HYPH	denotes	-
T11005	34865-34868	NN	denotes	cre
T11002	34869-34873	NNS	denotes	mice
T11007	34874-34881	VBG	denotes	lacking
T11008	34882-34885	NN	denotes	MTF
T11009	34885-34886	HYPH	denotes	-
T11010	34886-34887	CD	denotes	1
T10999	34888-34892	VBD	denotes	were
T11011	34893-34896	RB	denotes	not
T11012	34897-34905	JJ	denotes	suitable
T11013	34906-34908	TO	denotes	to
T11014	34909-34916	VB	denotes	analyze
T11015	34917-34920	NN	denotes	Afp
T11016	34921-34931	NN	denotes	expression
T11017	34931-34932	.	denotes	.
T11018	34932-35151	sentence	denotes	Cebpa is expressed in the adult liver as well as in other tissues (67), but the present microarray data revealed no significant expression differences in livers from adult Mtf1loxP and Mtf1Mx-cre mice (data not shown).
T11019	34933-34938	NN	denotes	Cebpa
T11021	34939-34941	VBZ	denotes	is
T11020	34942-34951	VBN	denotes	expressed
T11022	34952-34954	IN	denotes	in
T11023	34955-34958	DT	denotes	the
T11025	34959-34964	JJ	denotes	adult
T11024	34965-34970	NN	denotes	liver
T11026	34971-34973	RB	denotes	as
T11028	34974-34978	RB	denotes	well
T11027	34979-34981	IN	denotes	as
T11029	34982-34984	IN	denotes	in
T11030	34985-34990	JJ	denotes	other
T11031	34991-34998	NNS	denotes	tissues
T11032	34999-35000	-LRB-	denotes	(
T11033	35000-35002	CD	denotes	67
T11034	35002-35003	-RRB-	denotes	)
T11035	35003-35005	,	denotes	,
T11036	35005-35008	CC	denotes	but
T11037	35009-35012	DT	denotes	the
T11039	35013-35020	JJ	denotes	present
T11040	35021-35031	NN	denotes	microarray
T11038	35032-35036	NNS	denotes	data
T11041	35037-35045	VBD	denotes	revealed
T11042	35046-35048	DT	denotes	no
T11044	35049-35060	JJ	denotes	significant
T11045	35061-35071	NN	denotes	expression
T11043	35072-35083	NNS	denotes	differences
T11046	35084-35086	IN	denotes	in
T11047	35087-35093	NNS	denotes	livers
T11048	35094-35098	IN	denotes	from
T11049	35099-35104	JJ	denotes	adult
T11051	35105-35113	NN	denotes	Mtf1loxP
T11052	35114-35117	CC	denotes	and
T11053	35118-35124	NN	denotes	Mtf1Mx
T11055	35124-35125	HYPH	denotes	-
T11054	35125-35128	NN	denotes	cre
T11050	35129-35133	NNS	denotes	mice
T11056	35134-35135	-LRB-	denotes	(
T11058	35135-35139	NNS	denotes	data
T11059	35140-35143	RB	denotes	not
T11057	35144-35149	VBN	denotes	shown
T11060	35149-35150	-RRB-	denotes	)
T11061	35150-35151	.	denotes	.
T11062	35151-35288	sentence	denotes	Therefore, MTF-1 may affect Cebpa expression only during embryonal development, perhaps in combination with as yet unidentified factors.
T11063	35152-35161	RB	denotes	Therefore
T11065	35161-35163	,	denotes	,
T11066	35163-35166	NN	denotes	MTF
T11067	35166-35167	HYPH	denotes	-
T11068	35167-35168	CD	denotes	1
T11069	35169-35172	MD	denotes	may
T11064	35173-35179	VB	denotes	affect
T11070	35180-35185	NN	denotes	Cebpa
T11071	35186-35196	NN	denotes	expression
T11072	35197-35201	RB	denotes	only
T11073	35202-35208	IN	denotes	during
T11074	35209-35218	JJ	denotes	embryonal
T11075	35219-35230	NN	denotes	development
T11076	35230-35232	,	denotes	,
T11077	35232-35239	RB	denotes	perhaps
T11078	35240-35242	IN	denotes	in
T11079	35243-35254	NN	denotes	combination
T11080	35255-35259	IN	denotes	with
T11081	35260-35262	RB	denotes	as
T11082	35263-35266	RB	denotes	yet
T11083	35267-35279	JJ	denotes	unidentified
T11084	35280-35287	NNS	denotes	factors
T11085	35287-35288	.	denotes	.
T11086	35288-35388	sentence	denotes	The present study confirms and extends the role of MTF-1 as an important stress response regulator.
T11087	35289-35292	DT	denotes	The
T11089	35293-35300	JJ	denotes	present
T11088	35301-35306	NN	denotes	study
T11090	35307-35315	VBZ	denotes	confirms
T11091	35316-35319	CC	denotes	and
T11092	35320-35327	VBZ	denotes	extends
T11093	35328-35331	DT	denotes	the
T11094	35332-35336	NN	denotes	role
T11095	35337-35339	IN	denotes	of
T11096	35340-35343	NN	denotes	MTF
T11097	35343-35344	HYPH	denotes	-
T11098	35344-35345	CD	denotes	1
T11099	35346-35348	IN	denotes	as
T11100	35349-35351	DT	denotes	an
T11102	35352-35361	JJ	denotes	important
T11103	35362-35368	NN	denotes	stress
T11104	35369-35377	NN	denotes	response
T11101	35378-35387	NN	denotes	regulator
T11105	35387-35388	.	denotes	.
T11106	35388-35625	sentence	denotes	We have identified and preliminarily characterized four target genes of MTF-1 in the adult mouse liver: in the case of Sepw1, MTF-1 is required to maintain basal expression, supporting a role of mouse MTF-1 in oxidative stress response.
T11107	35389-35391	PRP	denotes	We
T11109	35392-35396	VBP	denotes	have
T11108	35397-35407	VBN	denotes	identified
T11111	35408-35411	CC	denotes	and
T11112	35412-35425	RB	denotes	preliminarily
T11113	35426-35439	VBN	denotes	characterized
T11114	35440-35444	CD	denotes	four
T11116	35445-35451	NN	denotes	target
T11115	35452-35457	NNS	denotes	genes
T11117	35458-35460	IN	denotes	of
T11118	35461-35464	NN	denotes	MTF
T11119	35464-35465	HYPH	denotes	-
T11120	35465-35466	CD	denotes	1
T11121	35467-35469	IN	denotes	in
T11122	35470-35473	DT	denotes	the
T11124	35474-35479	JJ	denotes	adult
T11125	35480-35485	NN	denotes	mouse
T11123	35486-35491	NN	denotes	liver
T11126	35491-35493	:	denotes	:
T11127	35493-35495	IN	denotes	in
T11128	35496-35499	DT	denotes	the
T11129	35500-35504	NN	denotes	case
T11130	35505-35507	IN	denotes	of
T11131	35508-35513	NN	denotes	Sepw1
T11132	35513-35515	,	denotes	,
T11133	35515-35518	NN	denotes	MTF
T11134	35518-35519	HYPH	denotes	-
T11135	35519-35520	CD	denotes	1
T11136	35521-35523	VBZ	denotes	is
T11110	35524-35532	VBN	denotes	required
T11137	35533-35535	TO	denotes	to
T11138	35536-35544	VB	denotes	maintain
T11139	35545-35550	JJ	denotes	basal
T11140	35551-35561	NN	denotes	expression
T11141	35561-35563	,	denotes	,
T11142	35563-35573	VBG	denotes	supporting
T11143	35574-35575	DT	denotes	a
T11144	35576-35580	NN	denotes	role
T11145	35581-35583	IN	denotes	of
T11146	35584-35589	NN	denotes	mouse
T11147	35590-35593	NN	denotes	MTF
T11148	35593-35594	HYPH	denotes	-
T11149	35594-35595	CD	denotes	1
T11150	35596-35598	IN	denotes	in
T11151	35599-35608	JJ	denotes	oxidative
T11153	35609-35615	NN	denotes	stress
T11152	35616-35624	NN	denotes	response
T11154	35624-35625	.	denotes	.
T11155	35625-35710	sentence	denotes	In addition, MTF-1 contributes to the cadmium-induced expression of Ndrg1 and Csrp1.
T11156	35626-35628	IN	denotes	In
T11158	35629-35637	NN	denotes	addition
T11159	35637-35639	,	denotes	,
T11160	35639-35642	NN	denotes	MTF
T11161	35642-35643	HYPH	denotes	-
T11162	35643-35644	CD	denotes	1
T11157	35645-35656	VBZ	denotes	contributes
T11163	35657-35659	IN	denotes	to
T11164	35660-35663	DT	denotes	the
T11166	35664-35671	NN	denotes	cadmium
T11168	35671-35672	HYPH	denotes	-
T11167	35672-35679	VBN	denotes	induced
T11165	35680-35690	NN	denotes	expression
T11169	35691-35693	IN	denotes	of
T11170	35694-35699	NN	denotes	Ndrg1
T11171	35700-35703	CC	denotes	and
T11172	35704-35709	NN	denotes	Csrp1
T11173	35709-35710	.	denotes	.
T11174	35710-35866	sentence	denotes	Furthermore, MTF-1 helps to repress the basal expression of Slc39a10, in contrast to its role as transcriptional activator for genes like Mt1, Mt2 or Znt1.
T11175	35711-35722	RB	denotes	Furthermore
T11177	35722-35724	,	denotes	,
T11178	35724-35727	NN	denotes	MTF
T11179	35727-35728	HYPH	denotes	-
T11180	35728-35729	CD	denotes	1
T11176	35730-35735	VBZ	denotes	helps
T11181	35736-35738	TO	denotes	to
T11182	35739-35746	VB	denotes	repress
T11183	35747-35750	DT	denotes	the
T11185	35751-35756	JJ	denotes	basal
T11184	35757-35767	NN	denotes	expression
T11186	35768-35770	IN	denotes	of
T11187	35771-35779	NN	denotes	Slc39a10
T11188	35779-35781	,	denotes	,
T11189	35781-35783	IN	denotes	in
T11190	35784-35792	NN	denotes	contrast
T11191	35793-35795	IN	denotes	to
T11192	35796-35799	PRP$	denotes	its
T11193	35800-35804	NN	denotes	role
T11194	35805-35807	IN	denotes	as
T11195	35808-35823	JJ	denotes	transcriptional
T11196	35824-35833	NN	denotes	activator
T11197	35834-35837	IN	denotes	for
T11198	35838-35843	NNS	denotes	genes
T11199	35844-35848	IN	denotes	like
T11200	35849-35852	NN	denotes	Mt1
T11201	35852-35854	,	denotes	,
T11202	35854-35857	NN	denotes	Mt2
T11203	35858-35860	CC	denotes	or
T11204	35861-35865	NN	denotes	Znt1
T11205	35865-35866	.	denotes	.
T11206	35866-35979	sentence	denotes	Thus the same transcription factor apparently serves as an activator or repressor, depending on the target gene.
T11207	35867-35871	RB	denotes	Thus
T11209	35872-35875	DT	denotes	the
T11211	35876-35880	JJ	denotes	same
T11212	35881-35894	NN	denotes	transcription
T11210	35895-35901	NN	denotes	factor
T11213	35902-35912	RB	denotes	apparently
T11208	35913-35919	VBZ	denotes	serves
T11214	35920-35922	IN	denotes	as
T11215	35923-35925	DT	denotes	an
T11216	35926-35935	NN	denotes	activator
T11217	35936-35938	CC	denotes	or
T11218	35939-35948	NN	denotes	repressor
T11219	35948-35950	,	denotes	,
T11220	35950-35959	VBG	denotes	depending
T11221	35960-35962	IN	denotes	on
T11222	35963-35966	DT	denotes	the
T11224	35967-35973	NN	denotes	target
T11223	35974-35978	NN	denotes	gene
T11225	35978-35979	.	denotes	.
T11226	35979-36176	sentence	denotes	The comparison of liver gene expression of cadmium- and mock-treated mice also revealed a number of genes that were responsive to cadmium exposure, independent of the presence or absence of MTF-1.
T11227	35980-35983	DT	denotes	The
T11228	35984-35994	NN	denotes	comparison
T11230	35995-35997	IN	denotes	of
T11231	35998-36003	NN	denotes	liver
T11232	36004-36008	NN	denotes	gene
T11233	36009-36019	NN	denotes	expression
T11234	36020-36022	IN	denotes	of
T11235	36023-36030	NN	denotes	cadmium
T11237	36030-36031	HYPH	denotes	-
T11238	36032-36035	CC	denotes	and
T11239	36036-36040	JJ	denotes	mock
T11240	36040-36041	HYPH	denotes	-
T11236	36041-36048	VBN	denotes	treated
T11241	36049-36053	NNS	denotes	mice
T11242	36054-36058	RB	denotes	also
T11229	36059-36067	VBD	denotes	revealed
T11243	36068-36069	DT	denotes	a
T11244	36070-36076	NN	denotes	number
T11245	36077-36079	IN	denotes	of
T11246	36080-36085	NNS	denotes	genes
T11247	36086-36090	WDT	denotes	that
T11248	36091-36095	VBD	denotes	were
T11249	36096-36106	JJ	denotes	responsive
T11250	36107-36109	IN	denotes	to
T11251	36110-36117	NN	denotes	cadmium
T11252	36118-36126	NN	denotes	exposure
T11253	36126-36128	,	denotes	,
T11254	36128-36139	JJ	denotes	independent
T11255	36140-36142	IN	denotes	of
T11256	36143-36146	DT	denotes	the
T11257	36147-36155	NN	denotes	presence
T11258	36156-36158	CC	denotes	or
T11259	36159-36166	NN	denotes	absence
T11260	36167-36169	IN	denotes	of
T11261	36170-36173	NN	denotes	MTF
T11262	36173-36174	HYPH	denotes	-
T11263	36174-36175	CD	denotes	1
T11264	36175-36176	.	denotes	.
T11265	36176-36443	sentence	denotes	Evidence suggests that the production of reactive oxygen species is a major effect of acute cadmium toxicity (68,69), and exposure of cultured cells or animals to cadmium is associated with depletion of reduced glutathione, lipid peroxidation and DNA damage (70–73).
T11266	36177-36185	NN	denotes	Evidence
T11268	36186-36194	VBZ	denotes	suggests
T11269	36195-36199	IN	denotes	that
T11271	36200-36203	DT	denotes	the
T11272	36204-36214	NN	denotes	production
T11273	36215-36217	IN	denotes	of
T11274	36218-36226	JJ	denotes	reactive
T11276	36227-36233	NN	denotes	oxygen
T11275	36234-36241	NNS	denotes	species
T11270	36242-36244	VBZ	denotes	is
T11277	36245-36246	DT	denotes	a
T11279	36247-36252	JJ	denotes	major
T11278	36253-36259	NN	denotes	effect
T11280	36260-36262	IN	denotes	of
T11281	36263-36268	JJ	denotes	acute
T11283	36269-36276	NN	denotes	cadmium
T11282	36277-36285	NN	denotes	toxicity
T11284	36286-36287	-LRB-	denotes	(
T11286	36287-36289	CD	denotes	68
T11287	36289-36290	,	denotes	,
T11285	36290-36292	CD	denotes	69
T11288	36292-36293	-RRB-	denotes	)
T11289	36293-36295	,	denotes	,
T11290	36295-36298	CC	denotes	and
T11291	36299-36307	NN	denotes	exposure
T11292	36308-36310	IN	denotes	of
T11293	36311-36319	VBN	denotes	cultured
T11294	36320-36325	NNS	denotes	cells
T11295	36326-36328	CC	denotes	or
T11296	36329-36336	NNS	denotes	animals
T11297	36337-36339	IN	denotes	to
T11298	36340-36347	NN	denotes	cadmium
T11267	36348-36350	VBZ	denotes	is
T11299	36351-36361	JJ	denotes	associated
T11300	36362-36366	IN	denotes	with
T11301	36367-36376	NN	denotes	depletion
T11302	36377-36379	IN	denotes	of
T11303	36380-36387	VBN	denotes	reduced
T11304	36388-36399	NN	denotes	glutathione
T11305	36399-36401	,	denotes	,
T11306	36401-36406	NN	denotes	lipid
T11307	36407-36419	NN	denotes	peroxidation
T11308	36420-36423	CC	denotes	and
T11309	36424-36427	NN	denotes	DNA
T11310	36428-36434	NN	denotes	damage
T11311	36435-36436	-LRB-	denotes	(
T11312	36436-36438	CD	denotes	70
T11313	36438-36439	SYM	denotes	–
T11314	36439-36441	CD	denotes	73
T11315	36441-36442	-RRB-	denotes	)
T11316	36442-36443	.	denotes	.
T11317	36443-36625	sentence	denotes	Oxidative stress and the subsequent restoration of cellular homeostasis have been shown to induce the expression of genes encoding acute-phase proteins and antioxidant enzymes (74).
T11318	36444-36453	JJ	denotes	Oxidative
T11319	36454-36460	NN	denotes	stress
T11321	36461-36464	CC	denotes	and
T11322	36465-36468	DT	denotes	the
T11324	36469-36479	JJ	denotes	subsequent
T11323	36480-36491	NN	denotes	restoration
T11325	36492-36494	IN	denotes	of
T11326	36495-36503	JJ	denotes	cellular
T11327	36504-36515	NN	denotes	homeostasis
T11328	36516-36520	VBP	denotes	have
T11329	36521-36525	VBN	denotes	been
T11320	36526-36531	VBN	denotes	shown
T11330	36532-36534	TO	denotes	to
T11331	36535-36541	VB	denotes	induce
T11332	36542-36545	DT	denotes	the
T11333	36546-36556	NN	denotes	expression
T11334	36557-36559	IN	denotes	of
T11335	36560-36565	NNS	denotes	genes
T11336	36566-36574	VBG	denotes	encoding
T11337	36575-36580	JJ	denotes	acute
T11339	36580-36581	HYPH	denotes	-
T11338	36581-36586	NN	denotes	phase
T11340	36587-36595	NN	denotes	proteins
T11341	36596-36599	CC	denotes	and
T11342	36600-36611	JJ	denotes	antioxidant
T11343	36612-36619	NNS	denotes	enzymes
T11344	36620-36621	-LRB-	denotes	(
T11345	36621-36623	CD	denotes	74
T11346	36623-36624	-RRB-	denotes	)
T11347	36624-36625	.	denotes	.
T11348	36625-36771	sentence	denotes	In mammals, cadmium tends to accumulate in the kidney and liver as a cadmium-metallothionein complex that has an extremely slow turnover (75,76).
T11349	36626-36628	IN	denotes	In
T11351	36629-36636	NNS	denotes	mammals
T11352	36636-36638	,	denotes	,
T11353	36638-36645	NN	denotes	cadmium
T11350	36646-36651	VBZ	denotes	tends
T11354	36652-36654	TO	denotes	to
T11355	36655-36665	VB	denotes	accumulate
T11356	36666-36668	IN	denotes	in
T11357	36669-36672	DT	denotes	the
T11358	36673-36679	NN	denotes	kidney
T11359	36680-36683	CC	denotes	and
T11360	36684-36689	NN	denotes	liver
T11361	36690-36692	IN	denotes	as
T11362	36693-36694	DT	denotes	a
T11364	36695-36702	NN	denotes	cadmium
T11366	36702-36703	HYPH	denotes	-
T11365	36703-36718	NN	denotes	metallothionein
T11363	36719-36726	NN	denotes	complex
T11367	36727-36731	WDT	denotes	that
T11368	36732-36735	VBZ	denotes	has
T11369	36736-36738	DT	denotes	an
T11371	36739-36748	RB	denotes	extremely
T11372	36749-36753	JJ	denotes	slow
T11370	36754-36762	NN	denotes	turnover
T11373	36763-36764	-LRB-	denotes	(
T11375	36764-36766	CD	denotes	75
T11376	36766-36767	,	denotes	,
T11374	36767-36769	CD	denotes	76
T11377	36769-36770	-RRB-	denotes	)
T11378	36770-36771	.	denotes	.
T11379	36771-36853	sentence	denotes	Furthermore, metallothioneins provide protection against oxidative stress (1,17).
T11380	36772-36783	RB	denotes	Furthermore
T11382	36783-36785	,	denotes	,
T11383	36785-36801	NNS	denotes	metallothioneins
T11381	36802-36809	VBP	denotes	provide
T11384	36810-36820	NN	denotes	protection
T11385	36821-36828	IN	denotes	against
T11386	36829-36838	JJ	denotes	oxidative
T11387	36839-36845	NN	denotes	stress
T11388	36846-36847	-LRB-	denotes	(
T11390	36847-36848	CD	denotes	1
T11391	36848-36849	,	denotes	,
T11389	36849-36851	CD	denotes	17
T11392	36851-36852	-RRB-	denotes	)
T11393	36852-36853	.	denotes	.
T11394	36853-36971	sentence	denotes	In addition to metallothioneins, glutathione was postulated as a first line of defense against cadmium toxicity (77).
T11395	36854-36856	IN	denotes	In
T11397	36857-36865	NN	denotes	addition
T11398	36866-36868	IN	denotes	to
T11399	36869-36885	NNS	denotes	metallothioneins
T11400	36885-36887	,	denotes	,
T11401	36887-36898	NN	denotes	glutathione
T11402	36899-36902	VBD	denotes	was
T11396	36903-36913	VBN	denotes	postulated
T11403	36914-36916	IN	denotes	as
T11404	36917-36918	DT	denotes	a
T11406	36919-36924	JJ	denotes	first
T11405	36925-36929	NN	denotes	line
T11407	36930-36932	IN	denotes	of
T11408	36933-36940	NN	denotes	defense
T11409	36941-36948	IN	denotes	against
T11410	36949-36956	NN	denotes	cadmium
T11411	36957-36965	NN	denotes	toxicity
T11412	36966-36967	-LRB-	denotes	(
T11413	36967-36969	CD	denotes	77
T11414	36969-36970	-RRB-	denotes	)
T11415	36970-36971	.	denotes	.
T11416	36971-37135	sentence	denotes	Glutathione efficiently complexes cadmium (78) and scavenges free radicals and other reactive oxygen species directly, and indirectly via enzymatic reactions (45).
T11417	36972-36983	NN	denotes	Glutathione
T11419	36984-36995	RB	denotes	efficiently
T11418	36996-37005	VBZ	denotes	complexes
T11420	37006-37013	NN	denotes	cadmium
T11421	37014-37015	-LRB-	denotes	(
T11422	37015-37017	CD	denotes	78
T11423	37017-37018	-RRB-	denotes	)
T11424	37019-37022	CC	denotes	and
T11425	37023-37032	VBZ	denotes	scavenges
T11426	37033-37037	JJ	denotes	free
T11427	37038-37046	NNS	denotes	radicals
T11428	37047-37050	CC	denotes	and
T11429	37051-37056	JJ	denotes	other
T11431	37057-37065	JJ	denotes	reactive
T11432	37066-37072	NN	denotes	oxygen
T11430	37073-37080	NNS	denotes	species
T11433	37081-37089	RB	denotes	directly
T11434	37089-37091	,	denotes	,
T11435	37091-37094	CC	denotes	and
T11436	37095-37105	RB	denotes	indirectly
T11437	37106-37109	IN	denotes	via
T11438	37110-37119	JJ	denotes	enzymatic
T11439	37120-37129	NNS	denotes	reactions
T11440	37130-37131	-LRB-	denotes	(
T11441	37131-37133	CD	denotes	45
T11442	37133-37134	-RRB-	denotes	)
T11443	37134-37135	.	denotes	.
T11444	37135-37230	sentence	denotes	In such reactions, glutathione is oxidized and has to be regenerated by glutathione reductase.
T11445	37136-37138	IN	denotes	In
T11447	37139-37143	JJ	denotes	such
T11448	37144-37153	NNS	denotes	reactions
T11449	37153-37155	,	denotes	,
T11450	37155-37166	NN	denotes	glutathione
T11451	37167-37169	VBZ	denotes	is
T11446	37170-37178	VBN	denotes	oxidized
T11452	37179-37182	CC	denotes	and
T11453	37183-37186	VBZ	denotes	has
T11454	37187-37189	TO	denotes	to
T11456	37190-37192	VB	denotes	be
T11455	37193-37204	VBN	denotes	regenerated
T11457	37205-37207	IN	denotes	by
T11458	37208-37219	NN	denotes	glutathione
T11459	37220-37229	NN	denotes	reductase
T11460	37229-37230	.	denotes	.
T11461	37230-37328	sentence	denotes	Also, glutathione-S-transferases mediate the conjugation of various electrophiles to glutathione.
T11462	37231-37235	RB	denotes	Also
T11464	37235-37237	,	denotes	,
T11465	37237-37248	NN	denotes	glutathione
T11467	37248-37249	HYPH	denotes	-
T11468	37249-37250	NN	denotes	S
T11469	37250-37251	HYPH	denotes	-
T11466	37251-37263	NNS	denotes	transferases
T11463	37264-37271	VBP	denotes	mediate
T11470	37272-37275	DT	denotes	the
T11471	37276-37287	NN	denotes	conjugation
T11472	37288-37290	IN	denotes	of
T11473	37291-37298	JJ	denotes	various
T11474	37299-37312	NNS	denotes	electrophiles
T11475	37313-37315	IN	denotes	to
T11476	37316-37327	NN	denotes	glutathione
T11477	37327-37328	.	denotes	.
T11478	37328-37466	sentence	denotes	The observed cadmium-induced upregulation of Gclc, Gsr and Gstm4 supports the importance of glutathione in the cellular cadmium response.
T11479	37329-37332	DT	denotes	The
T11481	37333-37341	VBN	denotes	observed
T11482	37342-37349	NN	denotes	cadmium
T11484	37349-37350	HYPH	denotes	-
T11483	37350-37357	VBN	denotes	induced
T11480	37358-37370	NN	denotes	upregulation
T11486	37371-37373	IN	denotes	of
T11487	37374-37378	NN	denotes	Gclc
T11488	37378-37380	,	denotes	,
T11489	37380-37383	NN	denotes	Gsr
T11490	37384-37387	CC	denotes	and
T11491	37388-37393	NN	denotes	Gstm4
T11485	37394-37402	VBZ	denotes	supports
T11492	37403-37406	DT	denotes	the
T11493	37407-37417	NN	denotes	importance
T11494	37418-37420	IN	denotes	of
T11495	37421-37432	NN	denotes	glutathione
T11496	37433-37435	IN	denotes	in
T11497	37436-37439	DT	denotes	the
T11499	37440-37448	JJ	denotes	cellular
T11500	37449-37456	NN	denotes	cadmium
T11498	37457-37465	NN	denotes	response
T11501	37465-37466	.	denotes	.
T11502	37466-37758	sentence	denotes	The enhanced sensitivity to cadmium toxicity that we found for mouse embryonic fibroblasts upon a combination of Mtf1 deletion and depletion of glutathione further corroborates the importance of MTF-1 and its target genes as well as reduced glutathione for an efficient anti-cadmium defense.
T11503	37467-37470	DT	denotes	The
T11505	37471-37479	VBN	denotes	enhanced
T11504	37480-37491	NN	denotes	sensitivity
T11507	37492-37494	IN	denotes	to
T11508	37495-37502	NN	denotes	cadmium
T11509	37503-37511	NN	denotes	toxicity
T11510	37512-37516	WDT	denotes	that
T11512	37517-37519	PRP	denotes	we
T11511	37520-37525	VBD	denotes	found
T11513	37526-37529	IN	denotes	for
T11514	37530-37535	NN	denotes	mouse
T11516	37536-37545	JJ	denotes	embryonic
T11515	37546-37557	NNS	denotes	fibroblasts
T11517	37558-37562	IN	denotes	upon
T11518	37563-37564	DT	denotes	a
T11519	37565-37576	NN	denotes	combination
T11520	37577-37579	IN	denotes	of
T11521	37580-37584	NN	denotes	Mtf1
T11522	37585-37593	NN	denotes	deletion
T11523	37594-37597	CC	denotes	and
T11524	37598-37607	NN	denotes	depletion
T11525	37608-37610	IN	denotes	of
T11526	37611-37622	NN	denotes	glutathione
T11527	37623-37630	RB	denotes	further
T11506	37631-37643	VBZ	denotes	corroborates
T11528	37644-37647	DT	denotes	the
T11529	37648-37658	NN	denotes	importance
T11530	37659-37661	IN	denotes	of
T11531	37662-37665	NN	denotes	MTF
T11532	37665-37666	HYPH	denotes	-
T11533	37666-37667	CD	denotes	1
T11534	37668-37671	CC	denotes	and
T11535	37672-37675	PRP$	denotes	its
T11537	37676-37682	NN	denotes	target
T11536	37683-37688	NNS	denotes	genes
T11538	37689-37691	RB	denotes	as
T11540	37692-37696	RB	denotes	well
T11539	37697-37699	IN	denotes	as
T11541	37700-37707	VBN	denotes	reduced
T11542	37708-37719	NN	denotes	glutathione
T11543	37720-37723	IN	denotes	for
T11544	37724-37726	DT	denotes	an
T11546	37727-37736	JJ	denotes	efficient
T11547	37737-37749	JJ	denotes	anti-cadmium
T11545	37750-37757	NN	denotes	defense
T11548	37757-37758	.	denotes	.
T11549	37758-37975	sentence	denotes	Our data provide strong evidence for at least two branches of cellular anti-cadmium defense, one via MTF-1 and its target genes, notably metallothioneins, the other via glutathione, with an apparent overlap in Sepw1.
T11550	37759-37762	PRP$	denotes	Our
T11551	37763-37767	NNS	denotes	data
T11552	37768-37775	VBP	denotes	provide
T11553	37776-37782	JJ	denotes	strong
T11554	37783-37791	NN	denotes	evidence
T11555	37792-37795	IN	denotes	for
T11556	37796-37798	RB	denotes	at
T11558	37799-37804	RBS	denotes	least
T11557	37805-37808	CD	denotes	two
T11559	37809-37817	NNS	denotes	branches
T11560	37818-37820	IN	denotes	of
T11561	37821-37829	JJ	denotes	cellular
T11563	37830-37842	JJ	denotes	anti-cadmium
T11562	37843-37850	NN	denotes	defense
T11564	37850-37852	,	denotes	,
T11565	37852-37855	CD	denotes	one
T11566	37856-37859	IN	denotes	via
T11567	37860-37863	NN	denotes	MTF
T11568	37863-37864	HYPH	denotes	-
T11569	37864-37865	CD	denotes	1
T11570	37866-37869	CC	denotes	and
T11571	37870-37873	PRP$	denotes	its
T11573	37874-37880	NN	denotes	target
T11572	37881-37886	NNS	denotes	genes
T11574	37886-37888	,	denotes	,
T11575	37888-37895	RB	denotes	notably
T11576	37896-37912	NNS	denotes	metallothioneins
T11577	37912-37914	,	denotes	,
T11578	37914-37917	DT	denotes	the
T11579	37918-37923	JJ	denotes	other
T11580	37924-37927	IN	denotes	via
T11581	37928-37939	NN	denotes	glutathione
T11582	37939-37941	,	denotes	,
T11583	37941-37945	IN	denotes	with
T11584	37946-37948	DT	denotes	an
T11586	37949-37957	JJ	denotes	apparent
T11585	37958-37965	NN	denotes	overlap
T11587	37966-37968	IN	denotes	in
T11588	37969-37974	NN	denotes	Sepw1
T11589	37974-37975	.	denotes	.
R10	T245	T243	pobj	defense,of
R100	T344	T342	compound	knockout,mice
R1000	T1680	T1669	nsubjpass	genes,induced
R1001	T1681	T1680	acl	involved,genes
R1002	T1682	T1681	prep	in,involved
R1003	T1683	T1684	compound	glutathione,metabolism
R1004	T1684	T1682	pobj	metabolism,in
R1005	T1685	T1669	auxpass	are,induced
R1006	T1686	T1669	punct	.,induced
R1007	T1688	T1689	amod	Further,studies
R1008	T1689	T1690	nsubj	studies,confirmed
R1009	T1691	T1692	det	a,defense
R101	T345	T342	cc	and,mice
R1010	T1692	T1690	dobj	defense,confirmed
R1011	T1693	T1692	amod	dual,defense
R1012	T1694	T1692	amod	anti-cadmium,defense
R1013	T1695	T1692	punct	", ",defense
R1014	T1696	T1692	appos	one,defense
R1015	T1697	T1696	prep	via,one
R1016	T1698	T1697	pobj	glutathione,via
R1017	T1699	T1696	cc	and,one
R1018	T1700	T1701	det	another,one
R1019	T1701	T1696	conj	one,one
R102	T346	T347	compound	control,littermates
R1020	T1702	T1701	prep	via,one
R1021	T1703	T1702	pobj	MTF,via
R1022	T1704	T1703	punct	-,MTF
R1023	T1705	T1703	nummod	1,MTF
R1024	T1706	T1703	cc	and,MTF
R1025	T1707	T1708	poss	its,genes
R1026	T1708	T1703	conj	genes,MTF
R1027	T1709	T1708	compound	target,genes
R1028	T1712	T1711	pobj	metallothioneins,including
R1029	T1710	T1708	punct	", ",genes
R103	T347	T342	conj	littermates,mice
R1030	T1713	T1690	punct	.,confirmed
R1031	T1711	T1708	prep	including,genes
R1032	T1759	T1758	prep	of,Generation
R1033	T1760	T1761	nmod	Mtf1,mice
R1034	T1761	T1759	pobj	mice,of
R1035	T1762	T1763	amod	conditional,knockout
R1036	T1763	T1761	compound	knockout,mice
R1037	T1764	T1761	cc	and,mice
R1038	T1765	T1766	npadvmod	liver,specific
R1039	T1766	T1768	amod	specific,deletion
R104	T348	T337	auxpass	were,treated
R1040	T1767	T1766	punct	-,specific
R1041	T1768	T1761	conj	deletion,mice
R1042	T1770	T1771	nmod	Mtf1,mice
R1043	T1771	T1774	nsubjpass	mice,generated
R1044	T1772	T1773	amod	conditional,knockout
R1045	T1773	T1771	compound	knockout,mice
R1046	T1775	T1774	auxpass	were,generated
R1047	T1776	T1774	prep	in,generated
R1048	T1777	T1776	pobj	collaboration,in
R1049	T1778	T1777	prep	with,collaboration
R105	T349	T350	preconj	both,mock
R1050	T1779	T1780	compound	Dr,Leviten
R1051	T1780	T1778	pobj	Leviten,with
R1052	T1781	T1780	compound	Michael,Leviten
R1053	T1782	T1783	punct	(,Carlos
R1054	T1783	T1780	parataxis	Carlos,Leviten
R1055	T1784	T1783	compound	San,Carlos
R1056	T1785	T1783	punct	", ",Carlos
R1057	T1786	T1783	npadvmod	CA,Carlos
R1058	T1787	T1783	punct	),Carlos
R1059	T1788	T1774	punct	.,generated
R106	T350	T337	amod	mock,treated
R1060	T1790	T1791	nummod	Two,clones
R1061	T1791	T1793	nsubjpass	clones,used
R1062	T1792	T1791	amod	genomic,clones
R1063	T1794	T1791	acl	containing,clones
R1064	T1795	T1796	nmod	exons,3
R1065	T1796	T1794	dobj	3,containing
R1066	T1797	T1796	prep	to,3
R1067	T1798	T1797	pobj	6,to
R1068	T1799	T1796	prep	of,3
R1069	T1800	T1799	pobj	Mtf1,of
R107	T351	T350	punct	-,mock
R1070	T1801	T1793	auxpass	were,used
R1071	T1802	T1803	aux	to,construct
R1072	T1803	T1793	advcl	construct,used
R1073	T1804	T1805	det	a,vector
R1074	T1805	T1803	dobj	vector,construct
R1075	T1806	T1807	compound	gene,targeting
R1076	T1807	T1805	compound	targeting,vector
R1077	T1808	T1803	prep	for,construct
R1078	T1809	T1810	amod	homologous,recombination
R1079	T1810	T1808	pobj	recombination,for
R108	T352	T350	cc	or,mock
R1080	T1811	T1812	punct	(,Data
R1081	T1812	T1803	parataxis	Data,construct
R1082	T1813	T1812	amod	Supplementary,Data
R1083	T1814	T1812	punct	),Data
R1084	T1815	T1793	punct	.,used
R1085	T1817	T1818	det	A,cassette
R1086	T1818	T1821	nsubjpass	cassette,cloned
R1087	T1819	T1818	compound	neomycin,cassette
R1088	T1820	T1818	compound	resistance,cassette
R1089	T1821	T1833	ccomp	cloned,cloned
R109	T353	T350	conj	cadmium,mock
R1090	T1822	T1818	punct	(,cassette
R1091	T1823	T1824	compound	PGK,neo
R1092	T1824	T1818	appos	neo,cassette
R1093	T1825	T1824	punct	-,neo
R1094	T1826	T1818	punct	),cassette
R1095	T1827	T1818	acl	flanked,cassette
R1096	T1828	T1827	agent	by,flanked
R1097	T1829	T1830	nummod	two,sites
R1098	T1830	T1828	pobj	sites,by
R1099	T1831	T1830	compound	loxP,sites
R11	T246	T245	prep	in,defense
R110	T354	T337	punct	-,treated
R1100	T1832	T1821	auxpass	was,cloned
R1101	T1834	T1821	prep	into,cloned
R1102	T1835	T1836	det	the,site
R1103	T1836	T1834	pobj	site,into
R1104	T1837	T1836	compound	SacI,site
R1105	T1838	T1836	npadvmod	5,site
R1106	T1839	T1838	punct	′,5
R1107	T1840	T1838	prep	of,5
R1108	T1841	T1840	pobj	exon,of
R1109	T1842	T1841	nummod	3,exon
R111	T355	T337	cc	and,treated
R1110	T1843	T1841	prep	of,exon
R1111	T1844	T1843	pobj	Mtf1,of
R1112	T1845	T1833	punct	", ",cloned
R1113	T1846	T1847	det	the,site
R1114	T1847	T1833	nsubjpass	site,cloned
R1115	T1848	T1847	amod	third,site
R1116	T1849	T1847	compound	loxP,site
R1117	T1850	T1833	auxpass	was,cloned
R1118	T1851	T1833	prep	into,cloned
R1119	T1852	T1853	det	the,site
R112	T356	T357	npadvmod	liver,specific
R1120	T1853	T1851	pobj	site,into
R1121	T1854	T1853	compound	ScaI,site
R1122	T1855	T1853	npadvmod	3,site
R1123	T1856	T1855	punct	′,3
R1124	T1857	T1855	prep	of,3
R1125	T1858	T1857	pobj	exon,of
R1126	T1859	T1858	nummod	4,exon
R1127	T1860	T1833	punct	.,cloned
R1128	T1862	T1863	det	A,cassette
R1129	T1863	T1869	nsubjpass	cassette,inserted
R113	T357	T359	amod	specific,transcription
R1130	T1864	T1865	nmod	thymidine,kinase
R1131	T1865	T1863	nmod	kinase,cassette
R1132	T1866	T1865	punct	(,kinase
R1133	T1867	T1865	appos	TK,kinase
R1134	T1868	T1863	punct	),cassette
R1135	T1870	T1869	auxpass	was,inserted
R1136	T1871	T1869	prep	in,inserted
R1137	T1872	T1873	det	the,site
R1138	T1873	T1871	pobj	site,in
R1139	T1874	T1873	compound	HpaI,site
R114	T358	T357	punct	-,specific
R1140	T1875	T1873	npadvmod	3,site
R1141	T1876	T1875	punct	′,3
R1142	T1877	T1875	prep	of,3
R1143	T1878	T1877	pobj	exon,of
R1144	T1879	T1878	nummod	6,exon
R1145	T1880	T1869	punct	.,inserted
R1146	T1882	T1883	nummod	129,cells
R1147	T1883	T1885	nsubjpass	cells,electroporated
R1148	T1884	T1883	compound	ES,cells
R1149	T1886	T1885	auxpass	were,electroporated
R115	T359	T360	nsubjpass	transcription,analyzed
R1150	T1887	T1885	prep	with,electroporated
R1151	T1888	T1889	det	the,vector
R1152	T1889	T1887	pobj	vector,with
R1153	T1890	T1889	amod	linearized,vector
R1154	T1891	T1889	compound	targeting,vector
R1155	T1892	T1889	punct	", ",vector
R1156	T1893	T1889	acl	selected,vector
R1157	T1894	T1893	prep	in,selected
R1158	T1895	T1896	det	the,presence
R1159	T1896	T1894	pobj	presence,in
R116	T360	T337	conj	analyzed,treated
R1160	T1897	T1896	prep	of,presence
R1161	T1898	T1897	pobj	G418,of
R1162	T1899	T1898	cc	and,G418
R1163	T1900	T1898	conj	FIAU,G418
R1164	T1901	T1885	punct	", ",electroporated
R1165	T1902	T1885	cc	and,electroporated
R1166	T1903	T1885	conj	screened,electroporated
R1167	T1904	T1903	prep	for,screened
R1168	T1905	T1906	amod	correct,events
R1169	T1906	T1904	pobj	events,for
R117	T361	T360	auxpass	was,analyzed
R1170	T1907	T1906	compound	integration,events
R1171	T1908	T1903	prep	by,screened
R1172	T1909	T1910	nmod	PCR,analysis
R1173	T1910	T1908	pobj	analysis,by
R1174	T1911	T1909	cc	and,PCR
R1175	T1912	T1913	compound	Southern,blot
R1176	T1913	T1909	conj	blot,PCR
R1177	T1914	T1885	punct	.,electroporated
R1178	T1916	T1917	amod	Transient,expression
R1179	T1917	T1918	nsubj	expression,led
R118	T362	T360	punct	.,analyzed
R1180	T1919	T1917	prep	of,expression
R1181	T1920	T1921	compound	Cre,recombinase
R1182	T1921	T1919	pobj	recombinase,of
R1183	T1922	T1918	prep	to,led
R1184	T1923	T1922	pobj	removal,to
R1185	T1924	T1923	prep	of,removal
R1186	T1925	T1926	det	the,cassette
R1187	T1926	T1924	pobj	cassette,of
R1188	T1927	T1928	compound	PGK,neo
R1189	T1928	T1926	compound	neo,cassette
R119	T364	T365	prep	Besides,emerged
R1190	T1929	T1928	punct	-,neo
R1191	T1930	T1918	punct	", ",led
R1192	T1931	T1918	cc	and,led
R1193	T1932	T1933	nsubjpass	mice,generated
R1194	T1933	T1918	conj	generated,led
R1195	T1934	T1932	acl	carrying,mice
R1196	T1935	T1936	det	the,allele
R1197	T1936	T1934	dobj	allele,carrying
R1198	T1937	T1936	amod	modified,allele
R1199	T1938	T1936	compound	Mtf1loxP,allele
R12	T247	T248	det	the,mouse
R120	T366	T367	det	the,genes
R1200	T1939	T1933	auxpass	were,generated
R1201	T1940	T1933	prep	by,generated
R1202	T1941	T1940	pobj	injection,by
R1203	T1942	T1941	prep	of,injection
R1204	T1943	T1944	amod	positive,clones
R1205	T1944	T1942	pobj	clones,of
R1206	T1945	T1941	prep	into,injection
R1207	T1946	T1947	nmod	C57Bl,blastocysts
R1208	T1947	T1945	pobj	blastocysts,into
R1209	T1948	T1946	punct	/,C57Bl
R121	T367	T364	pobj	genes,Besides
R1210	T1949	T1946	nummod	6,C57Bl
R1211	T1950	T1947	cc	and,blastocysts
R1212	T1951	T1952	amod	subsequent,crosses
R1213	T1952	T1947	conj	crosses,blastocysts
R1214	T1953	T1933	punct	.,generated
R1215	T1955	T1956	amod	Homozygous,animals
R1216	T1956	T1959	nsubjpass	animals,crossed
R1217	T1957	T1958	amod	conditional,knockout
R1218	T1958	T1956	compound	knockout,animals
R1219	T1960	T1956	punct	(,animals
R122	T368	T369	advmod	well,characterized
R1220	T1961	T1962	compound	Mtf1loxP,loxP
R1221	T1962	T1956	appos	loxP,animals
R1222	T1963	T1962	punct	/,loxP
R1223	T1964	T1959	punct	),crossed
R1224	T1965	T1959	auxpass	were,crossed
R1225	T1966	T1959	prep	with,crossed
R1226	T1967	T1968	det	the,line
R1227	T1968	T1966	pobj	line,with
R1228	T2074	T2075	compound	Cre,recombinase
R1229	T2075	T2076	npadvmod	recombinase,transgenic
R123	T369	T367	amod	characterized,genes
R1230	T2076	T1968	amod	transgenic,line
R1231	T2077	T2078	compound	Mx,cre
R1232	T2078	T1968	appos	cre,line
R1233	T2079	T2078	punct	-,cre
R1234	T2080	T2081	punct	(,gift
R1235	T2081	T1968	parataxis	gift,line
R1236	T2082	T2081	det	a,gift
R1237	T2083	T2081	prep	from,gift
R1238	T2084	T2085	compound	Prof.,Aguet
R1239	T2085	T2083	pobj	Aguet,from
R124	T370	T369	punct	-,characterized
R1240	T2086	T2085	compound	Michel,Aguet
R1241	T2087	T2081	punct	),gift
R1242	T2088	T2089	aux	to,obtain
R1243	T2089	T1959	advcl	obtain,crossed
R1244	T2090	T2091	det	an,line
R1245	T2091	T2089	dobj	line,obtain
R1246	T2092	T2091	amod	inducible,line
R1247	T2093	T2091	punct	", ",line
R1248	T2094	T2095	npadvmod	liver,specific
R1249	T2095	T2091	amod	specific,line
R125	T371	T367	compound	metallothionein,genes
R1250	T2096	T2095	punct	-,specific
R1251	T2097	T2098	compound	Mtf1,knockout
R1252	T2098	T2091	compound	knockout,line
R1253	T2099	T1959	punct	.,crossed
R1254	T2101	T2102	det	The,mice
R1255	T2102	T2103	nsubjpass	mice,genotyped
R1256	T2104	T2103	auxpass	were,genotyped
R1257	T2105	T2103	prep	by,genotyped
R1258	T2106	T2105	pobj	PCR,by
R1259	T2107	T2103	advcl	using,genotyped
R126	T372	T365	punct	", ",emerged
R1260	T2108	T2109	det	the,primers
R1261	T2109	T2107	dobj	primers,using
R1262	T2110	T2109	amod	following,primers
R1263	T2111	T2112	punct	(,Microsynth
R1264	T2112	T2109	parataxis	Microsynth,primers
R1265	T2113	T2112	punct	),Microsynth
R1266	T2114	T2103	punct	: ,genotyped
R1267	T2115	T2116	compound	Cre,recombinase
R1268	T2116	T2117	dep	recombinase,CCAGGTTACGGATATAGTTCATGAC
R1269	T2117	T2130	dep	CCAGGTTACGGATATAGTTCATGAC,CAGTCACAAGCAAATTACCAAACACTGCC
R127	T373	T374	amod	several,genes
R1270	T2118	T2117	punct	: ,CCAGGTTACGGATATAGTTCATGAC
R1271	T2119	T2120	nummod	5,CTATCCAGCAACATTTGGGCCAGC
R1272	T2120	T2117	dep	CTATCCAGCAACATTTGGGCCAGC,CCAGGTTACGGATATAGTTCATGAC
R1273	T2121	T2119	punct	′,5
R1274	T2122	T2120	punct	-,CTATCCAGCAACATTTGGGCCAGC
R1275	T2123	T2120	punct	-,CTATCCAGCAACATTTGGGCCAGC
R1276	T2124	T2120	nummod	3,CTATCCAGCAACATTTGGGCCAGC
R1277	T2125	T2120	punct	′,CTATCCAGCAACATTTGGGCCAGC
R1278	T2126	T2117	punct	;,CCAGGTTACGGATATAGTTCATGAC
R1279	T2127	T2117	nummod	5,CCAGGTTACGGATATAGTTCATGAC
R128	T374	T365	nsubj	genes,emerged
R1280	T2128	T2127	punct	′,5
R1281	T2129	T2117	punct	-,CCAGGTTACGGATATAGTTCATGAC
R1282	T2130	T2103	dep	CAGTCACAAGCAAATTACCAAACACTGCC,genotyped
R1283	T2131	T2117	punct	-,CCAGGTTACGGATATAGTTCATGAC
R1284	T2132	T2117	nummod	3,CCAGGTTACGGATATAGTTCATGAC
R1285	T2133	T2117	punct	′,CCAGGTTACGGATATAGTTCATGAC
R1286	T2134	T2130	punct	", ",CAGTCACAAGCAAATTACCAAACACTGCC
R1287	T2135	T2130	dep	Mtf1loxP,CAGTCACAAGCAAATTACCAAACACTGCC
R1288	T2136	T2135	cc	or,Mtf1loxP
R1289	T2137	T2138	amod	wild,type
R129	T375	T374	amod	new,genes
R1290	T2138	T2140	compound	type,allele
R1291	T2139	T2138	punct	-,type
R1292	T2140	T2135	conj	allele,Mtf1loxP
R1293	T2141	T2130	punct	: ,CAGTCACAAGCAAATTACCAAACACTGCC
R1294	T2142	T2143	nummod	5,CACACCCAGTTTGTGTATGTCTTC
R1295	T2143	T2130	dep	CACACCCAGTTTGTGTATGTCTTC,CAGTCACAAGCAAATTACCAAACACTGCC
R1296	T2144	T2142	punct	′,5
R1297	T2145	T2143	punct	-,CACACCCAGTTTGTGTATGTCTTC
R1298	T2146	T2143	punct	-,CACACCCAGTTTGTGTATGTCTTC
R1299	T2147	T2143	nummod	3,CACACCCAGTTTGTGTATGTCTTC
R13	T248	T246	pobj	mouse,in
R130	T376	T374	nmod	MTF,genes
R1300	T2148	T2143	punct	′,CACACCCAGTTTGTGTATGTCTTC
R1301	T2149	T2130	punct	;,CAGTCACAAGCAAATTACCAAACACTGCC
R1302	T2150	T2130	nummod	5,CAGTCACAAGCAAATTACCAAACACTGCC
R1303	T2151	T2150	punct	′,5
R1304	T2152	T2130	punct	-,CAGTCACAAGCAAATTACCAAACACTGCC
R1305	T2153	T2130	punct	-,CAGTCACAAGCAAATTACCAAACACTGCC
R1306	T2154	T2130	nummod	3,CAGTCACAAGCAAATTACCAAACACTGCC
R1307	T2155	T2130	punct	′,CAGTCACAAGCAAATTACCAAACACTGCC
R1308	T2156	T2103	punct	.,genotyped
R1309	T2173	T2174	compound	Animal,treatment
R131	T377	T376	punct	-,MTF
R1310	T2176	T2177	prep	At,received
R1311	T2178	T2179	nummod	8,weeks
R1312	T2179	T2176	pobj	weeks,At
R1313	T2180	T2179	prep	of,weeks
R1314	T2181	T2180	pobj	age,of
R1315	T2182	T2177	punct	", ",received
R1316	T2183	T2184	amod	male,mice
R1317	T2184	T2177	nsubj	mice,received
R1318	T2185	T2186	compound	Mtf1loxP,loxP
R1319	T2186	T2184	compound	loxP,mice
R132	T378	T376	nummod	1,MTF
R1320	T2187	T2186	punct	/,loxP
R1321	T2188	T2184	acl	harboring,mice
R1322	T2189	T2190	det	the,transgene
R1323	T2190	T2188	dobj	transgene,harboring
R1324	T2191	T2192	compound	Mx,cre
R1325	T2192	T2190	compound	cre,transgene
R1326	T2193	T2192	punct	-,cre
R1327	T2194	T2190	punct	(,transgene
R1328	T2195	T2196	compound	Mtf1loxP,cre
R1329	T2196	T2190	appos	cre,transgene
R133	T379	T374	compound	target,genes
R1330	T2197	T2196	punct	/,cre
R1331	T2198	T2196	compound	loxP,cre
R1332	T2199	T2196	compound	Mx,cre
R1333	T2200	T2196	punct	-,cre
R1334	T2201	T2196	punct	", ",cre
R1335	T2202	T2203	advmod	abbr.,cre
R1336	T2203	T2196	appos	cre,cre
R1337	T2204	T2203	punct	: ,cre
R1338	T2205	T2203	compound	Mtf1Mx,cre
R1339	T2206	T2203	punct	-,cre
R134	T380	T374	prep	with,genes
R1340	T2207	T2190	punct	),transgene
R1341	T2208	T2190	cc	and,transgene
R1342	T2209	T2210	compound	control,littermates
R1343	T2210	T2190	conj	littermates,transgene
R1344	T2211	T2210	prep	without,littermates
R1345	T2212	T2211	pobj	transgene,without
R1346	T2213	T2210	punct	(,littermates
R1347	T2214	T2215	compound	Mtf1loxP,loxP
R1348	T2215	T2210	appos	loxP,littermates
R1349	T2216	T2215	punct	/,loxP
R135	T381	T382	compound	MRE,motifs
R1350	T2217	T2215	punct	", ",loxP
R1351	T2218	T2219	advmod	abbr.,Mtf1loxP
R1352	T2219	T2215	appos	Mtf1loxP,loxP
R1353	T2220	T2219	punct	: ,Mtf1loxP
R1354	T2221	T2177	punct	),received
R1355	T2222	T2223	nummod	four,injections
R1356	T2223	T2177	dobj	injections,received
R1357	T2224	T2223	amod	intraperitoneal,injections
R1358	T2225	T2223	advmod	each,injections
R1359	T2226	T2223	prep	of,injections
R136	T382	T380	pobj	motifs,with
R1360	T2227	T2228	nummod	300,µg
R1361	T2228	T2229	nmod	µg,acid
R1362	T2229	T2226	pobj	acid,of
R1363	T2230	T2229	amod	synthetic,acid
R1364	T2231	T2232	amod	double,stranded
R1365	T2232	T2229	amod	stranded,acid
R1366	T2233	T2323	nummod	20,µmol
R1367	T2234	T2232	punct	-,stranded
R1368	T2235	T2229	nmod	RNA,acid
R1369	T2236	T2237	amod	polyinosinic,polycytidylic
R137	T383	T382	prep	in,motifs
R1370	T2237	T2229	amod	polycytidylic,acid
R1371	T2238	T2237	punct	–,polycytidylic
R1372	T2239	T2240	punct	[,Sigma
R1373	T2240	T2229	parataxis	Sigma,acid
R1374	T2241	T2242	compound	pI,pC
R1375	T2242	T2240	dep	pC,Sigma
R1376	T2243	T2242	punct	–,pC
R1377	T2244	T2240	punct	;,Sigma
R1378	T2245	T2240	punct	;,Sigma
R1379	T2246	T2240	prep	in,Sigma
R138	T384	T385	det	the,region
R1380	T2247	T2248	det	a,volume
R1381	T2248	T2246	pobj	volume,in
R1382	T2249	T2248	prep	of,volume
R1383	T2250	T2251	nummod	60,µl
R1384	T2251	T2249	pobj	µl,of
R1385	T2252	T2253	npadvmod	phosphate,buffered
R1386	T2253	T2255	amod	buffered,saline
R1387	T2254	T2253	punct	-,buffered
R1388	T2255	T2251	appos	saline,µl
R1389	T2256	T2255	punct	(,saline
R139	T385	T383	pobj	region,in
R1390	T2257	T2255	appos	PBS,saline
R1391	T2258	T2240	punct	),Sigma
R1392	T2259	T2240	punct	],Sigma
R1393	T2260	T2177	prep	at,received
R1394	T2261	T2262	nummod	3,day
R1395	T2262	T2263	compound	day,intervals
R1396	T2263	T2260	pobj	intervals,at
R1397	T2264	T2177	punct	.,received
R1398	T2266	T2267	advmod	Only,in
R1399	T2267	T2268	prep	in,received
R14	T249	T240	punct	: ,branches
R140	T386	T385	compound	promoter,region
R1400	T2269	T2270	det	the,case
R1401	T2270	T2267	pobj	case,in
R1402	T2271	T2270	prep	of,case
R1403	T2272	T2273	npadvmod	DNA,binding
R1404	T2273	T2275	amod	binding,studies
R1405	T2274	T2273	punct	-,binding
R1406	T2275	T2271	pobj	studies,of
R1407	T2276	T2275	prep	with,studies
R1408	T2277	T2278	compound	MRE,sequences
R1409	T2278	T2276	pobj	sequences,with
R141	T387	T365	punct	.,emerged
R1410	T2279	T2278	prep	from,sequences
R1411	T2280	T2281	nmod	MTF,candidates
R1412	T2281	T2279	pobj	candidates,from
R1413	T2282	T2280	punct	-,MTF
R1414	T2283	T2280	nummod	1,MTF
R1415	T2284	T2285	compound	target,gene
R1416	T2285	T2281	compound	gene,candidates
R1417	T2286	T2268	punct	", ",received
R1418	T2287	T2288	det	the,mice
R1419	T2288	T2268	nsubj	mice,received
R142	T389	T390	nsubjpass	MTF,required
R1420	T2289	T2288	compound	control,mice
R1421	T2290	T2291	det	no,injections
R1422	T2291	T2268	dobj	injections,received
R1423	T2292	T2293	compound	pI,pC
R1424	T2293	T2291	compound	pC,injections
R1425	T2294	T2293	punct	–,pC
R1426	T2295	T2268	punct	.,received
R1427	T2297	T2298	prep	For,received
R1428	T2299	T2297	pobj	experiments,For
R1429	T2300	T2299	prep	with,experiments
R143	T391	T389	punct	-,MTF
R1430	T2301	T2302	compound	metal,treatment
R1431	T2302	T2300	pobj	treatment,with
R1432	T2303	T2298	punct	", ",received
R1433	T2304	T2298	nsubj	mice,received
R1434	T2305	T2306	nummod	2,days
R1435	T2306	T2307	npadvmod	days,after
R1436	T2307	T2298	prep	after,received
R1437	T2308	T2309	det	the,treatment
R1438	T2309	T2307	pobj	treatment,after
R1439	T2310	T2309	amod	last,treatment
R144	T392	T389	nummod	1,MTF
R1440	T2311	T2312	compound	pI,pC
R1441	T2312	T2309	compound	pC,treatment
R1442	T2313	T2312	punct	–,pC
R1443	T2314	T2315	det	a,injection
R1444	T2315	T2298	dobj	injection,received
R1445	T2316	T2317	amod	subcutaneous,s.c.
R1446	T2317	T2315	amod	s.c.,injection
R1447	T2318	T2317	punct	(,s.c.
R1448	T2319	T2315	punct	),injection
R1449	T2320	T2315	prep	of,injection
R145	T393	T390	auxpass	is,required
R1450	T2321	T2322	preconj	either,CdSO4
R1451	T2322	T2320	pobj	CdSO4,of
R1452	T2323	T2322	nmod	µmol,CdSO4
R1453	T2324	T2325	punct	/,weight
R1454	T2325	T2323	prep	weight,µmol
R1455	T2326	T2325	compound	kg,weight
R1456	T2327	T2325	compound	body,weight
R1457	T2328	T2329	punct	(,treatment
R1458	T2329	T2322	parataxis	treatment,CdSO4
R1459	T2330	T2331	nummod	2,mM
R146	T394	T390	prep	for,required
R1460	T2331	T2332	compound	mM,CdSO4
R1461	T2332	T2329	dep	CdSO4,treatment
R1462	T2333	T2332	prep	in,CdSO4
R1463	T2334	T2333	pobj	H2O,in
R1464	T2335	T2329	punct	;,treatment
R1465	T2336	T2329	compound	cadmium,treatment
R1466	T2337	T2329	punct	),treatment
R1467	T2338	T2322	cc	or,CdSO4
R1468	T2339	T2340	nummod	10,ml
R1469	T2340	T2341	nmod	ml,H2O
R147	T395	T396	det	the,expression
R1470	T2341	T2322	conj	H2O,CdSO4
R1471	T2342	T2343	punct	/,weight
R1472	T2343	T2340	prep	weight,ml
R1473	T2344	T2343	compound	kg,weight
R1474	T2345	T2343	compound	body,weight
R1475	T2346	T2347	punct	(,treatment
R1476	T2347	T2341	parataxis	treatment,H2O
R1477	T2348	T2347	amod	mock,treatment
R1478	T2349	T2347	punct	),treatment
R1479	T2350	T2351	nummod	6,h
R148	T396	T394	pobj	expression,for
R1480	T2351	T2352	npadvmod	h,before
R1481	T2352	T2298	prep	before,received
R1482	T2353	T2352	pcomp	sacrificing,before
R1483	T2354	T2353	dobj	them,sacrificing
R1484	T2355	T2298	punct	.,received
R1487	T2531	T2532	compound	Microarray,analysis
R1488	T2533	T2532	cc	and,analysis
R1489	T2534	T2535	compound	data,processing
R149	T397	T396	amod	basal,expression
R1490	T2535	T2532	conj	processing,analysis
R1491	T2537	T2538	amod	Total,RNA
R1492	T2538	T2539	nsubjpass	RNA,isolated
R1493	T2540	T2539	auxpass	was,isolated
R1494	T2541	T2539	prep	from,isolated
R1495	T2542	T2543	compound	liver,tissue
R1496	T2543	T2541	pobj	tissue,from
R1497	T2544	T2543	prep	of,tissue
R1498	T2545	T2546	compound	pI,pC
R1499	T2546	T2548	npadvmod	pC,induced
R15	T250	T251	nmod	MTF,metallothioneins
R150	T398	T396	prep	of,expression
R1500	T2547	T2546	punct	–,pC
R1501	T2548	T2550	amod	induced,mice
R1502	T2549	T2548	punct	-,induced
R1503	T2550	T2544	pobj	mice,of
R1504	T2551	T2550	punct	", ",mice
R1505	T2552	T2553	amod	mock,treated
R1506	T2553	T2550	amod	treated,mice
R1507	T2554	T2552	punct	-,mock
R1508	T2555	T2552	cc	or,mock
R1509	T2556	T2552	conj	cadmium,mock
R151	T399	T400	nmod	selenoprotein,gene
R1510	T2557	T2553	punct	-,treated
R1511	T2558	T2559	nmod	Mtf1Mx,cre
R1512	T2559	T2550	nmod	cre,mice
R1513	T2560	T2559	punct	-,cre
R1514	T2561	T2559	cc	and,cre
R1515	T2562	T2559	conj	Mtf1loxP,cre
R1516	T2563	T2564	punct	(,male
R1517	T2564	T2544	parataxis	male,of
R1518	T2565	T2566	nsubj	n,3
R1519	T2566	T2564	ccomp	3,male
R152	T400	T398	pobj	gene,of
R1520	T2567	T2566	punct	=,3
R1521	T2568	T2566	prep	per,3
R1522	T2569	T2568	pobj	genotype,per
R1523	T2570	T2569	cc	and,genotype
R1524	T2571	T2572	amod	respective,treatment
R1525	T2572	T2569	conj	treatment,genotype
R1526	T2573	T2564	punct	;,male
R1527	T2574	T2564	det	all,male
R1528	T2575	T2564	punct	),male
R1529	T2576	T2577	advmod	essentially,described
R153	T401	T400	nmod	W,gene
R1530	T2577	T2539	advcl	described,isolated
R1531	T2578	T2577	mark	as,described
R1532	T2579	T2577	agent	by,described
R1533	T2580	T2579	pobj	Chomczynski,by
R1534	T2581	T2580	cc	and,Chomczynski
R1535	T2582	T2580	conj	Sacchi,Chomczynski
R1536	T2583	T2584	punct	(,27
R1537	T2584	T2577	parataxis	27,described
R1538	T2585	T2584	punct	),27
R1539	T2586	T2539	punct	.,isolated
R154	T402	T400	punct	", ",gene
R1540	T2588	T2589	compound	Gene,expression
R1541	T2589	T2590	compound	expression,analysis
R1542	T2590	T2591	nsubjpass	analysis,performed
R1543	T2592	T2591	auxpass	was,performed
R1544	T2593	T2591	prep	in,performed
R1545	T2594	T2595	det	the,Center
R1546	T2595	T2593	pobj	Center,in
R1547	T2596	T2597	compound	Functional,Genomics
R1548	T2597	T2595	compound	Genomics,Center
R1549	T2598	T2595	npadvmod	Zurich,Center
R155	T403	T400	nmod	muscle,gene
R1550	T2599	T2591	advcl	using,performed
R1551	T2600	T2601	nmod	GeneChip®,Arrays
R1552	T2601	T2599	dobj	Arrays,using
R1553	T2602	T2603	nmod	Mouse,Genome
R1554	T2603	T2601	nmod	Genome,Arrays
R1555	T2604	T2603	nummod	430,Genome
R1556	T2605	T2601	nummod	2.0,Arrays
R1557	T2606	T2607	punct	(,Affymetrix
R1558	T2607	T2601	parataxis	Affymetrix,Arrays
R1559	T2608	T2607	punct	),Affymetrix
R156	T404	T400	nummod	1,gene
R1560	T2609	T2599	prep	according,using
R1561	T2610	T2609	prep	to,according
R1562	T2611	T2612	det	the,manufacturer
R1563	T2612	T2613	poss	manufacturer,instructions
R1564	T2613	T2610	pobj	instructions,to
R1565	T2614	T2612	case	's,manufacturer
R1566	T2615	T2613	cc	and,instructions
R1567	T2616	T2617	det	the,reagents
R1568	T2617	T2613	conj	reagents,instructions
R1569	T2618	T2617	amod	following,reagents
R157	T405	T400	punct	(,gene
R1570	T2619	T2617	cc	and,reagents
R1571	T2620	T2617	conj	conditions,reagents
R1572	T2621	T2591	punct	.,performed
R1573	T2623	T2624	nsubjpass	cDNA,synthesized
R1574	T2625	T2624	auxpass	was,synthesized
R1575	T2626	T2624	prep	with,synthesized
R1576	T2627	T2628	nmod	SuperScript,Kit
R1577	T2628	T2626	pobj	Kit,with
R1578	T2629	T2627	punct	™,SuperScript
R1579	T2630	T2631	amod	Double,Stranded
R158	T406	T400	appos	Sepw1,gene
R1580	T2631	T2628	amod	Stranded,Kit
R1581	T2632	T2631	punct	-,Stranded
R1582	T2633	T2628	compound	cDNA,Kit
R1583	T2634	T2628	compound	Synthesis,Kit
R1584	T2635	T2636	punct	(,Invitrogen
R1585	T2636	T2628	parataxis	Invitrogen,Kit
R1586	T2637	T2636	punct	),Invitrogen
R1587	T2638	T2624	punct	", ",synthesized
R1588	T2639	T2624	advcl	using,synthesized
R1589	T2640	T2641	nummod	15,µg
R159	T407	T400	punct	),gene
R1590	T2641	T2642	nmod	µg,RNA
R1591	T2642	T2639	dobj	RNA,using
R1592	T2643	T2642	amod	total,RNA
R1593	T2644	T2624	punct	.,synthesized
R1594	T2646	T2647	advmod	In,vitro
R1595	T2647	T2648	amod	vitro,transcription
R1596	T2648	T2649	nsubjpass	transcription,performed
R1597	T2650	T2649	auxpass	was,performed
R1598	T2651	T2649	prep	with,performed
R1599	T2652	T2653	nmod	BioArray,Kit
R16	T251	T240	appos	metallothioneins,branches
R160	T408	T409	dep	that,encodes
R1600	T2653	T2651	pobj	Kit,with
R1601	T2654	T2652	punct	™,BioArray
R1602	T2655	T2656	nmod	High,Yield
R1603	T2656	T2653	nmod	Yield,Kit
R1604	T2657	T2656	punct	™,Yield
R1605	T2658	T2653	compound	RNA,Kit
R1606	T2659	T2653	compound	Transcript,Kit
R1607	T2660	T2653	compound	Labeling,Kit
R1608	T2661	T2662	punct	(,Enzo
R1609	T2662	T2653	parataxis	Enzo,Kit
R161	T409	T400	relcl	encodes,gene
R1610	T2663	T2662	punct	),Enzo
R1611	T2664	T2653	cc	and,Kit
R1612	T2665	T2666	quantmod	3.5,6
R1613	T2666	T2668	nummod	6,µg
R1614	T2667	T2666	quantmod	to,6
R1615	T2668	T2653	conj	µg,Kit
R1616	T2669	T2668	prep	of,µg
R1617	T2670	T2671	det	each,cDNA
R1618	T2671	T2669	pobj	cDNA,of
R1619	T2672	T2649	punct	.,performed
R162	T410	T411	det	a,protein
R1620	T2674	T2675	compound	Clean,up
R1621	T2675	T2677	nsubjpass	up,done
R1622	T2676	T2675	punct	-,up
R1623	T2678	T2675	prep	of,up
R1624	T2679	T2680	preconj	both,cDNA
R1625	T2680	T2681	nmod	cDNA,samples
R1626	T2681	T2678	pobj	samples,of
R1627	T2682	T2680	cc	and,cDNA
R1628	T2683	T2680	conj	cRNA,cDNA
R1629	T2684	T2677	auxpass	was,done
R163	T411	T409	dobj	protein,encodes
R1630	T2685	T2677	advcl	using,done
R1631	T2686	T2687	compound	GeneChip®,Module
R1632	T2687	T2685	dobj	Module,using
R1633	T2688	T2687	compound	Sample,Module
R1634	T2689	T2690	compound	Clean,up
R1635	T2690	T2687	compound	up,Module
R1636	T2691	T2690	punct	-,up
R1637	T2692	T2693	punct	(,Affymetrix
R1638	T2693	T2687	parataxis	Affymetrix,Module
R1639	T2694	T2693	punct	),Affymetrix
R164	T412	T413	npadvmod	glutathione,binding
R1640	T2695	T2677	punct	.,done
R1641	T2697	T2698	prep	For,used
R1642	T2699	T2700	det	the,washing
R1643	T2700	T2697	pobj	washing,For
R1644	T2701	T2700	amod	automated,washing
R1645	T2702	T2700	cc	and,washing
R1646	T2703	T2700	conj	staining,washing
R1647	T2704	T2700	prep	in,washing
R1648	T2705	T2706	det	the,station
R1649	T2706	T2704	pobj	station,in
R165	T413	T411	amod	binding,protein
R1650	T2707	T2706	compound	Affymetrix,station
R1651	T2708	T2706	compound	fluidics,station
R1652	T2709	T2706	nummod	450,station
R1653	T2710	T2698	punct	", ",used
R1654	T2711	T2712	det	the,Ws2v4_450
R1655	T2712	T2698	nsubjpass	Ws2v4_450,used
R1656	T2713	T2712	compound	protocol,Ws2v4_450
R1657	T2714	T2712	compound	EukGE,Ws2v4_450
R1658	T2715	T2712	punct	-,Ws2v4_450
R1659	T2716	T2698	auxpass	was,used
R166	T414	T413	punct	-,binding
R1660	T2717	T2698	punct	.,used
R1661	T2719	T2720	det	The,arrays
R1662	T2720	T2722	nsubjpass	arrays,scanned
R1663	T2721	T2720	compound	probe,arrays
R1664	T2723	T2722	auxpass	were,scanned
R1665	T2724	T2722	prep	with,scanned
R1666	T2725	T2726	det	the,scanner
R1667	T2726	T2724	pobj	scanner,with
R1668	T2727	T2728	nmod	Affymetrix,GS
R1669	T2728	T2726	nmod	GS,scanner
R167	T415	T413	cc	and,binding
R1670	T2729	T2728	nummod	3000,GS
R1671	T2730	T2722	punct	.,scanned
R1672	T2732	T2733	amod	Raw,data
R1673	T2733	T2734	nsubj	data,are
R1674	T2735	T2734	acomp	available,are
R1675	T2736	T2734	prep	at,are
R1676	T2737	T2736	pobj	ArrayExpress,at
R1677	T2738	T2739	punct	(,MEXP
R1678	T2739	T2734	parataxis	MEXP,are
R1679	T2740	T2739	punct	", ",MEXP
R168	T416	T417	amod	putative,antioxidant
R1680	T2741	T2742	compound	accession,number
R1681	T2742	T2739	compound	number,MEXP
R1682	T2743	T2739	compound	E,MEXP
R1683	T2744	T2739	punct	-,MEXP
R1684	T2745	T2739	punct	-,MEXP
R1685	T2746	T2739	nummod	438,MEXP
R1686	T2747	T2739	punct	),MEXP
R1687	T2748	T2734	punct	.,are
R1688	T2750	T2751	compound	Data,analysis
R1689	T2751	T2752	nsubjpass	analysis,performed
R169	T417	T413	conj	antioxidant,binding
R1690	T2753	T2752	auxpass	was,performed
R1691	T2754	T2752	prep	with,performed
R1692	T2755	T2756	nmod	GeneSpring,software
R1693	T2756	T2754	pobj	software,with
R1694	T2757	T2756	nummod	6.1,software
R1695	T2758	T2759	punct	(,Genetics
R1696	T2759	T2756	parataxis	Genetics,software
R1697	T2760	T2759	compound	Silicon,Genetics
R1698	T2761	T2759	punct	),Genetics
R1699	T2762	T2752	punct	", ",performed
R17	T252	T250	punct	-,MTF
R170	T418	T409	punct	", ",encodes
R1700	T2763	T2752	advcl	applying,performed
R1701	T2764	T2765	det	a,level
R1702	T2765	T2763	dobj	level,applying
R1703	T2766	T2765	compound	significance,level
R1704	T2767	T2768	nsubj	P,0.05
R1705	T2768	T2765	appos	0.05,level
R1706	T2769	T2768	punct	≤,0.05
R1707	T2770	T2752	punct	.,performed
R1708	T2772	T2773	advmod	Furthermore,used
R1709	T2774	T2773	punct	", ",used
R171	T419	T409	advcl	supporting,encodes
R1710	T2775	T2776	amod	multiple,correction
R1711	T2776	T2773	nsubjpass	correction,used
R1712	T2777	T2776	compound	testing,correction
R1713	T2778	T2773	auxpass	was,used
R1714	T2779	T2773	prep	in,used
R1715	T2780	T2779	pobj	addition,in
R1716	T2781	T2782	aux	to,obtain
R1717	T2782	T2773	advcl	obtain,used
R1718	T2783	T2784	npadvmod	cadmium,responsive
R1719	T2784	T2786	amod	responsive,genes
R172	T420	T421	det	a,role
R1720	T2785	T2784	punct	-,responsive
R1721	T2786	T2782	dobj	genes,obtain
R1722	T2787	T2786	punct	", ",genes
R1723	T2788	T2789	npadvmod	MTF,independent
R1724	T2789	T2786	amod	independent,genes
R1725	T2790	T2788	punct	-,MTF
R1726	T2791	T2788	nummod	1,MTF
R1727	T2792	T2789	punct	-,independent
R1728	T2793	T2773	punct	.,used
R1729	T2795	T2796	nsubjpass	Genes,considered
R173	T421	T419	dobj	role,supporting
R1730	T2797	T2796	auxpass	were,considered
R1731	T2798	T2799	aux	to,expressed
R1732	T2799	T2796	xcomp	expressed,considered
R1733	T2800	T2799	auxpass	be,expressed
R1734	T2801	T2799	advmod	differentially,expressed
R1735	T2802	T2803	mark	if,was
R1736	T2803	T2799	advcl	was,expressed
R1737	T2804	T2803	expl	there,was
R1738	T2805	T2806	advmod	at,least
R1739	T2806	T2807	advmod	least,2-fold
R174	T422	T421	prep	of,role
R1740	T2807	T2809	nummod	2-fold,difference
R1741	T2808	T2807	quantmod	a,2-fold
R1742	T2809	T2803	attr	difference,was
R1743	T2810	T2809	prep	in,difference
R1744	T2811	T2812	compound	expression,levels
R1745	T2812	T2810	pobj	levels,in
R1746	T2813	T2812	prep	of,levels
R1747	T2814	T2815	det	the,groups
R1748	T2815	T2813	pobj	groups,of
R1749	T2816	T2815	amod	compared,groups
R175	T423	T422	pobj	MTF,of
R1750	T2817	T2815	amod	experimental,groups
R1751	T2818	T2819	punct	(,genotype
R1752	T2819	T2803	parataxis	genotype,was
R1753	T2820	T2819	cc	and,genotype
R1754	T2821	T2822	amod	respective,treatment
R1755	T2822	T2819	conj	treatment,genotype
R1756	T2823	T2819	punct	),genotype
R1757	T2824	T2796	punct	.,considered
R1758	T2826	T2827	det	The,result
R1759	T2827	T2828	nsubjpass	result,considered
R176	T424	T423	punct	-,MTF
R1760	T2829	T2828	auxpass	was,considered
R1761	T2830	T2828	oprd	reliable,considered
R1762	T2831	T2832	mark	if,scored
R1763	T2832	T2828	advcl	scored,considered
R1764	T2833	T2834	compound	signal,values
R1765	T2834	T2832	nsubjpass	values,scored
R1766	T2835	T2834	prep	for,values
R1767	T2836	T2837	det	the,gene
R1768	T2837	T2835	pobj	gene,for
R1769	T2838	T2837	amod	respective,gene
R177	T425	T423	nummod	1,MTF
R1770	T2839	T2832	auxpass	were,scored
R1771	T2840	T2841	punct	‘,present
R1772	T2841	T2832	oprd	present,scored
R1773	T2842	T2832	punct	’,scored
R1774	T2843	T2844	advmod	at,least
R1775	T2844	T2845	advmod	least,for
R1776	T2845	T2828	prep	for,considered
R1777	T2846	T2847	det	all,mice
R1778	T2847	T2845	pobj	mice,for
R1779	T2848	T2847	prep	in,mice
R178	T426	T421	prep	in,role
R1780	T2849	T2850	nummod	one,group
R1781	T2850	T2848	pobj	group,in
R1782	T2851	T2850	amod	experimental,group
R1783	T2852	T2845	cc	or,for
R1784	T2853	T2845	conj	for,for
R1785	T2854	T2855	nummod	two,mice
R1786	T2855	T2853	pobj	mice,for
R1787	T2856	T2855	prep	in,mice
R1788	T2857	T2856	pobj	each,in
R1789	T2858	T2857	prep	of,each
R179	T427	T428	det	the,response
R1790	T2859	T2860	nummod	two,groups
R1791	T2860	T2858	pobj	groups,of
R1792	T2861	T2828	punct	.,considered
R1793	T2863	T2864	aux	To,screen
R1794	T2864	T2865	advcl	screen,obtained
R1795	T2866	T2864	prep	for,screen
R1796	T2867	T2868	det	the,presence
R1797	T2868	T2866	pobj	presence,for
R1798	T2869	T2868	prep	of,presence
R1799	T2870	T2871	det	the,sequence
R18	T253	T250	nummod	1,MTF
R180	T428	T426	pobj	response,in
R1800	T2871	T2869	pobj	sequence,of
R1801	T2872	T2871	compound	MRE,sequence
R1802	T2873	T2871	compound	core,sequence
R1803	T2874	T2871	compound	consensus,sequence
R1804	T2875	T2871	appos	TGCRCNC,sequence
R1805	T2876	T2868	prep	in,presence
R1806	T2877	T2878	det	the,region
R1807	T2878	T2876	pobj	region,in
R1808	T2879	T2878	compound	promoter,region
R1809	T2880	T2865	punct	", ",obtained
R181	T429	T428	amod	oxidative,response
R1810	T2881	T2882	det	the,sequences
R1811	T2882	T2865	nsubjpass	sequences,obtained
R1812	T2883	T2882	amod	upstream,sequences
R1813	T2884	T2882	prep	of,sequences
R1814	T2885	T2886	det	the,genes
R1815	T2886	T2884	pobj	genes,of
R1816	T2887	T2886	amod	respective,genes
R1817	T2888	T2865	auxpass	were,obtained
R1818	T2889	T2865	prep	from,obtained
R1819	T2890	T2891	det	the,Database
R182	T430	T428	compound	stress,response
R1820	T2891	T2889	pobj	Database,from
R1821	T2892	T2891	nmod	University,Database
R1822	T2893	T2892	prep	of,University
R1823	T2894	T2895	compound	California,Cruz
R1824	T2895	T2893	pobj	Cruz,of
R1825	T2896	T2895	compound	Santa,Cruz
R1826	T2897	T2892	punct	(,University
R1827	T2898	T2892	appos	UCSC,University
R1828	T2899	T2891	punct	),Database
R1829	T2900	T2891	compound	Genome,Database
R183	T431	T390	punct	.,required
R1830	T2901	T2891	compound	Browser,Database
R1831	T2902	T2903	punct	(,November
R1832	T2903	T2891	parataxis	November,Database
R1833	T2904	T2903	punct	;,November
R1834	T2905	T2903	nmod	October,November
R1835	T2906	T2903	punct	/,November
R1836	T2907	T2903	nummod	2004,November
R1837	T2908	T2903	punct	),November
R1838	T2909	T2910	punct	(,28
R1839	T2910	T2891	parataxis	28,Database
R184	T433	T434	advmod	Furthermore,mediates
R1840	T2911	T2910	punct	),28
R1841	T2912	T2865	punct	.,obtained
R1842	T2931	T2989	appos	TTCCGATGTGCCAAGTGTGGC,Csrp1
R1843	T2932	T2933	compound	RT,PCR
R1844	T2934	T2933	punct	–,PCR
R1845	T2936	T2937	det	All,PCRs
R1846	T2937	T2940	nsubjpass	PCRs,performed
R1847	T2938	T2937	compound	RT,PCRs
R1848	T2939	T2937	punct	–,PCRs
R1849	T2941	T2940	auxpass	were,performed
R185	T435	T434	punct	", ",mediates
R1850	T2942	T2940	prep	with,performed
R1851	T2943	T2944	compound	QIAGEN®,Kit
R1852	T2944	T2942	pobj	Kit,with
R1853	T2945	T2944	compound	OneStep,Kit
R1854	T2946	T2947	compound	RT,PCR
R1855	T2947	T2944	compound	PCR,Kit
R1856	T2948	T2947	punct	–,PCR
R1857	T2949	T2950	punct	(,QIAGEN
R1858	T2950	T2944	parataxis	QIAGEN,Kit
R1859	T2951	T2950	punct	),QIAGEN
R186	T436	T434	nsubj	MTF,mediates
R1860	T2952	T2940	prep	according,performed
R1861	T2953	T2952	prep	to,according
R1862	T2954	T2955	det	the,manufacturer
R1863	T2955	T2956	poss	manufacturer,instructions
R1864	T2956	T2953	pobj	instructions,to
R1865	T2957	T2955	case	's,manufacturer
R1866	T2958	T2940	punct	", ",performed
R1867	T2959	T2940	advcl	using,performed
R1868	T2960	T2961	quantmod	150,200
R1869	T2961	T2963	nummod	200,ng
R187	T437	T436	punct	-,MTF
R1870	T2962	T2961	punct	–,200
R1871	T2963	T2964	nmod	ng,RNA
R1872	T2964	T2959	dobj	RNA,using
R1873	T2965	T2966	compound	DNase,I
R1874	T2966	T2967	npadvmod	I,digested
R1875	T2967	T2964	amod	digested,RNA
R1876	T2968	T2967	punct	-,digested
R1877	T2969	T2964	amod	total,RNA
R1878	T2970	T2971	punct	(,isolation
R1879	T2971	T2959	parataxis	isolation,using
R188	T438	T436	nummod	1,MTF
R1880	T2972	T2971	compound	RNA,isolation
R1881	T2973	T2971	advcl	see,isolation
R1882	T2974	T2975	compound	microarray,analysis
R1883	T2975	T2973	dobj	analysis,see
R1884	T2976	T2971	punct	),isolation
R1885	T2977	T2940	punct	.,performed
R1886	T2979	T2980	det	The,reactions
R1887	T2980	T2981	nsubjpass	reactions,carried
R1888	T2982	T2981	auxpass	were,carried
R1889	T2983	T2981	prt	out,carried
R189	T439	T440	det	the,expression
R1890	T2984	T2981	advcl	using,carried
R1891	T2985	T2986	det	the,primers
R1892	T2986	T2984	dobj	primers,using
R1893	T2987	T2986	amod	following,primers
R1894	T2988	T2986	punct	: ,primers
R1895	T2989	T2986	appos	Csrp1,primers
R1896	T2990	T2989	punct	: ,Csrp1
R1897	T2991	T2931	nummod	5,TTCCGATGTGCCAAGTGTGGC
R1898	T2992	T2991	punct	′,5
R1899	T2993	T2931	punct	-,TTCCGATGTGCCAAGTGTGGC
R19	T254	T251	punct	/,metallothioneins
R190	T440	T434	dobj	expression,mediates
R1900	T2994	T2931	punct	-,TTCCGATGTGCCAAGTGTGGC
R1901	T2995	T2931	nummod	3,TTCCGATGTGCCAAGTGTGGC
R1902	T2996	T2931	punct	′,TTCCGATGTGCCAAGTGTGGC
R1903	T2997	T2931	punct	;,TTCCGATGTGCCAAGTGTGGC
R1904	T2998	T2999	nummod	5,AGTAGAGAGTGGACATTCAGC
R1905	T2999	T2931	appos	AGTAGAGAGTGGACATTCAGC,TTCCGATGTGCCAAGTGTGGC
R1906	T3000	T2998	punct	′,5
R1907	T3001	T2999	punct	-,AGTAGAGAGTGGACATTCAGC
R1908	T3002	T2999	punct	-,AGTAGAGAGTGGACATTCAGC
R1909	T3003	T2999	nummod	3,AGTAGAGAGTGGACATTCAGC
R191	T441	T442	npadvmod	cadmium,induced
R1910	T3004	T2999	punct	′,AGTAGAGAGTGGACATTCAGC
R1911	T3005	T2989	punct	", ",Csrp1
R1912	T3006	T3007	compound	hypoxanthin,phosphoribosyltransferase
R1913	T3007	T2989	appos	phosphoribosyltransferase,Csrp1
R1914	T3008	T3007	punct	-,phosphoribosyltransferase
R1915	T3009	T3007	compound	guanin,phosphoribosyltransferase
R1916	T3010	T3007	punct	-,phosphoribosyltransferase
R1917	T3011	T3007	punct	(,phosphoribosyltransferase
R1918	T3012	T3007	appos	Hprt,phosphoribosyltransferase
R1919	T3013	T3007	punct	),phosphoribosyltransferase
R192	T442	T440	amod	induced,expression
R1920	T3014	T3007	punct	: ,phosphoribosyltransferase
R1921	T3015	T3016	nummod	5,GCTGGTGAAAAGGACCTCTCG
R1922	T3016	T3007	appos	GCTGGTGAAAAGGACCTCTCG,phosphoribosyltransferase
R1923	T3017	T3015	punct	′,5
R1924	T3018	T3016	punct	-,GCTGGTGAAAAGGACCTCTCG
R1925	T3019	T3016	punct	-,GCTGGTGAAAAGGACCTCTCG
R1926	T3020	T3016	nummod	3,GCTGGTGAAAAGGACCTCTCG
R1927	T3021	T3016	punct	′,GCTGGTGAAAAGGACCTCTCG
R1928	T3022	T3016	punct	;,GCTGGTGAAAAGGACCTCTCG
R1929	T3023	T3024	nummod	5,CCACAGGACTAGGACACCTGC
R193	T443	T442	punct	-,induced
R1930	T3024	T3016	appos	CCACAGGACTAGGACACCTGC,GCTGGTGAAAAGGACCTCTCG
R1931	T3025	T3023	punct	′,5
R1932	T3026	T3024	punct	-,CCACAGGACTAGGACACCTGC
R1933	T3027	T3024	punct	-,CCACAGGACTAGGACACCTGC
R1934	T3028	T3024	nummod	3,CCACAGGACTAGGACACCTGC
R1935	T3029	T3024	punct	′,CCACAGGACTAGGACACCTGC
R1936	T3030	T2989	punct	", ",Csrp1
R1937	T3031	T2989	appos	Mtf1,Csrp1
R1938	T3032	T3031	punct	: ,Mtf1
R1939	T3033	T3034	nummod	5,GTGACTTTTGAGACTGTACTGAGTG
R194	T444	T440	prep	of,expression
R1940	T3034	T3031	appos	GTGACTTTTGAGACTGTACTGAGTG,Mtf1
R1941	T3035	T3033	punct	′,5
R1942	T3036	T3034	punct	-,GTGACTTTTGAGACTGTACTGAGTG
R1943	T3037	T3034	punct	-,GTGACTTTTGAGACTGTACTGAGTG
R1944	T3038	T3034	nummod	3,GTGACTTTTGAGACTGTACTGAGTG
R1945	T3039	T3034	punct	′,GTGACTTTTGAGACTGTACTGAGTG
R1946	T3040	T3034	punct	;,GTGACTTTTGAGACTGTACTGAGTG
R1947	T3041	T3042	nummod	5,CATGCCAAGAAACATTGAAGGTG
R1948	T3042	T3034	appos	CATGCCAAGAAACATTGAAGGTG,GTGACTTTTGAGACTGTACTGAGTG
R1949	T3043	T3041	punct	′,5
R195	T445	T446	nmod	N,myc
R1950	T3044	T3042	punct	-,CATGCCAAGAAACATTGAAGGTG
R1951	T3045	T3042	punct	-,CATGCCAAGAAACATTGAAGGTG
R1952	T3046	T3042	nummod	3,CATGCCAAGAAACATTGAAGGTG
R1953	T3047	T3042	punct	′,CATGCCAAGAAACATTGAAGGTG
R1954	T3048	T2989	punct	", ",Csrp1
R1955	T3049	T2989	appos	Ndrg1,Csrp1
R1956	T3050	T3049	punct	: ,Ndrg1
R1957	T3051	T3052	nummod	5,AGATACACAACAACGTGGAGG
R1958	T3052	T3049	appos	AGATACACAACAACGTGGAGG,Ndrg1
R1959	T3053	T3051	punct	′,5
R196	T446	T448	nmod	myc,gene
R1960	T3054	T3052	punct	-,AGATACACAACAACGTGGAGG
R1961	T3055	T3052	punct	-,AGATACACAACAACGTGGAGG
R1962	T3056	T3052	nummod	3,AGATACACAACAACGTGGAGG
R1963	T3057	T3052	punct	′,AGATACACAACAACGTGGAGG
R1964	T3058	T3052	punct	;,AGATACACAACAACGTGGAGG
R1965	T3059	T3060	nummod	5,TGTGCGAGCGGCTTCGGGGGC
R1966	T3060	T3052	appos	TGTGCGAGCGGCTTCGGGGGC,AGATACACAACAACGTGGAGG
R1967	T3061	T3059	punct	′,5
R1968	T3062	T3060	punct	-,TGTGCGAGCGGCTTCGGGGGC
R1969	T3063	T3060	punct	-,TGTGCGAGCGGCTTCGGGGGC
R197	T447	T446	punct	-,myc
R1970	T3064	T3060	nummod	3,TGTGCGAGCGGCTTCGGGGGC
R1971	T3065	T3060	punct	′,TGTGCGAGCGGCTTCGGGGGC
R1972	T3066	T2989	punct	", ",Csrp1
R1973	T3067	T2989	appos	Sepw1,Csrp1
R1974	T3068	T3067	punct	: ,Sepw1
R1975	T3069	T3070	nummod	5,TAGAGGCAGGGTCCTGAAAGC
R1976	T3070	T3067	appos	TAGAGGCAGGGTCCTGAAAGC,Sepw1
R1977	T3071	T3069	punct	′,5
R1978	T3072	T3070	punct	-,TAGAGGCAGGGTCCTGAAAGC
R1979	T3073	T3070	punct	-,TAGAGGCAGGGTCCTGAAAGC
R198	T448	T444	pobj	gene,of
R1980	T3074	T3070	nummod	3,TAGAGGCAGGGTCCTGAAAGC
R1981	T3075	T3070	punct	′,TAGAGGCAGGGTCCTGAAAGC
R1982	T3076	T3070	punct	;,TAGAGGCAGGGTCCTGAAAGC
R1983	T3077	T3078	nummod	5,ACACCTGGAAACATGGCTGCC
R1984	T3078	T3070	appos	ACACCTGGAAACATGGCTGCC,TAGAGGCAGGGTCCTGAAAGC
R1985	T3079	T3077	punct	′,5
R1986	T3080	T3078	punct	-,ACACCTGGAAACATGGCTGCC
R1987	T3081	T3078	punct	-,ACACCTGGAAACATGGCTGCC
R1988	T3082	T3078	nummod	3,ACACCTGGAAACATGGCTGCC
R1989	T3083	T3078	punct	′,ACACCTGGAAACATGGCTGCC
R199	T449	T450	advmod	downstream,regulated
R1990	T3084	T2989	punct	", ",Csrp1
R1991	T3085	T2989	appos	Slc39a10,Csrp1
R1992	T3086	T3085	punct	: ,Slc39a10
R1993	T3087	T3088	nummod	5,GCTGTGGCTGGTAGTAAAAGC
R1994	T3088	T3085	appos	GCTGTGGCTGGTAGTAAAAGC,Slc39a10
R1995	T3089	T3087	punct	′,5
R1996	T3090	T3088	punct	-,GCTGTGGCTGGTAGTAAAAGC
R1997	T3091	T3088	punct	-,GCTGTGGCTGGTAGTAAAAGC
R1998	T3092	T3088	nummod	3,GCTGTGGCTGGTAGTAAAAGC
R1999	T3093	T3088	punct	′,GCTGTGGCTGGTAGTAAAAGC
R20	T255	T251	cc	and,metallothioneins
R200	T450	T448	amod	regulated,gene
R2000	T3094	T3088	punct	;,GCTGTGGCTGGTAGTAAAAGC
R2001	T3095	T3096	nummod	5,GTGGCATGGGATGTAAACAGC
R2002	T3096	T3088	appos	GTGGCATGGGATGTAAACAGC,GCTGTGGCTGGTAGTAAAAGC
R2003	T3097	T3095	punct	′,5
R2004	T3098	T3096	punct	-,GTGGCATGGGATGTAAACAGC
R2005	T3099	T3096	punct	-,GTGGCATGGGATGTAAACAGC
R2006	T3100	T3096	nummod	3,GTGGCATGGGATGTAAACAGC
R2007	T3101	T3096	punct	′,GTGGCATGGGATGTAAACAGC
R2008	T3102	T2981	punct	.,carried
R2009	T3137	T3138	compound	S1,mapping
R201	T451	T448	nummod	1,gene
R2010	T3139	T3138	compound	nuclease,mapping
R2011	T3140	T3138	prep	of,mapping
R2012	T3141	T3140	pobj	transcripts,of
R2013	T3142	T3143	punct	(,analysis
R2014	T3143	T3138	parataxis	analysis,mapping
R2015	T3144	T3143	compound	S1,analysis
R2016	T3145	T3143	punct	),analysis
R2017	T3147	T3148	compound	S1,analysis
R2018	T3148	T3149	nsubjpass	analysis,performed
R2019	T3150	T3149	auxpass	was,performed
R202	T452	T448	punct	(,gene
R2020	T3151	T3152	mark	as,described
R2021	T3152	T3149	advcl	described,performed
R2022	T3153	T3152	advmod	previously,described
R2023	T3154	T3155	punct	(,29
R2024	T3155	T3152	parataxis	29,described
R2025	T3156	T3155	punct	),29
R2026	T3157	T3149	punct	", ",performed
R2027	T3158	T3149	advcl	using,performed
R2028	T3159	T3160	nummod	100,µg
R2029	T3160	T3161	nmod	µg,RNA
R203	T453	T448	appos	Ndrg1,gene
R2030	T3161	T3158	dobj	RNA,using
R2031	T3162	T3163	npadvmod	DNase,digested
R2032	T3163	T3161	amod	digested,RNA
R2033	T3164	T3162	nummod	I,DNase
R2034	T3165	T3163	punct	-,digested
R2035	T3166	T3161	amod	total,RNA
R2036	T3167	T3168	punct	(,see
R2037	T3168	T3161	parataxis	see,RNA
R2038	T3169	T3170	compound	RNA,isolation
R2039	T3170	T3168	nsubj	isolation,see
R204	T454	T448	punct	),gene
R2040	T3171	T3172	compound	microarray,analysis
R2041	T3172	T3168	dobj	analysis,see
R2042	T3173	T3168	punct	),see
R2043	T3174	T3149	punct	.,performed
R2044	T3176	T3177	det	The,gels
R2045	T3177	T3178	nsubjpass	gels,developed
R2046	T3179	T3178	auxpass	were,developed
R2047	T3180	T3178	advcl	using,developed
R2048	T3181	T3180	dobj	PhosphorImager,using
R2049	T3182	T3183	punct	(,Dynamics
R205	T455	T448	punct	", ",gene
R2050	T3183	T3178	parataxis	Dynamics,developed
R2051	T3184	T3183	compound	Molecular,Dynamics
R2052	T3185	T3183	punct	),Dynamics
R2053	T3186	T3178	punct	.,developed
R2054	T3188	T3189	compound	S1,analysis
R2055	T3189	T3190	nsubjpass	analysis,done
R2056	T3191	T3190	auxpass	was,done
R2057	T3192	T3190	prep	with,done
R2058	T3193	T3194	det	the,oligonucleotides
R2059	T3194	T3192	pobj	oligonucleotides,with
R206	T456	T457	dep	which,induced
R2060	T3195	T3194	amod	following,oligonucleotides
R2061	T3196	T3197	npadvmod	32P,labeled
R2062	T3197	T3194	amod	labeled,oligonucleotides
R2063	T3198	T3197	punct	-,labeled
R2064	T3199	T3194	punct	: ,oligonucleotides
R2065	T3200	T3201	compound	Hprt,S1
R2066	T3201	T3194	appos	S1,oligonucleotides
R2067	T3202	T3201	punct	: ,S1
R2068	T3203	T3204	nummod	5,TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG
R2069	T3204	T3201	appos	TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG,S1
R207	T457	T448	relcl	induced,gene
R2070	T3205	T3203	punct	′,5
R2071	T3206	T3204	punct	-,TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG
R2072	T3207	T3204	punct	-,TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG
R2073	T3208	T3204	nummod	3,TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG
R2074	T3209	T3204	punct	′,TCTTCAGTCTGATAAAATCTACAGTCATAGGAATGGATCTATCACTATTTCTATTCAGTGATTACATTAAAG
R2075	T3210	T3201	punct	", ",S1
R2076	T3211	T3212	compound	Sepw1,S1
R2077	T3212	T3201	appos	S1,S1
R2078	T3213	T3212	punct	: ,S1
R2079	T3214	T3215	nummod	5,TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC
R208	T458	T457	auxpass	is,induced
R2080	T3215	T3212	appos	TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC,S1
R2081	T3216	T3214	punct	′,5
R2082	T3217	T3215	punct	-,TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC
R2083	T3218	T3215	punct	-,TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC
R2084	T3219	T3215	nummod	3,TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC
R2085	T3220	T3215	punct	′,TTCAACCGGGAACACCTGGAAACATGGCTGCCTGTCTTCTTGAAGTCTTGAGGTGGAAAGGGAAAGCAAAGCAGGAGGGTTTTCCCACCC
R2086	T3221	T3190	punct	.,done
R2089	T3351	T3352	amod	Electrophoretic,shift
R209	T459	T457	agent	by,induced
R2090	T3352	T3354	compound	shift,assay
R2091	T3353	T3352	compound	mobility,shift
R2092	T3355	T3354	punct	(,assay
R2093	T3356	T3354	appos	EMSA,assay
R2094	T3357	T3354	punct	),assay
R2095	T3359	T3360	nsubjpass	Protein,extracted
R2096	T3361	T3360	auxpass	was,extracted
R2097	T3362	T3360	prep	from,extracted
R2098	T3363	T3364	compound	liver,tissue
R2099	T3364	T3362	pobj	tissue,from
R21	T256	T251	conj	glutathione,metallothioneins
R210	T460	T461	amod	several,conditions
R2100	T3365	T3360	prep	with,extracted
R2101	T3366	T3367	compound	T,PER
R2102	T3367	T3365	pobj	PER,with
R2103	T3368	T3367	punct	-,PER
R2104	T3369	T3367	punct	™,PER
R2105	T3370	T3371	compound	Tissue,Protein
R2106	T3371	T3372	compound	Protein,Extraction
R2107	T3372	T3373	compound	Extraction,Reagent
R2108	T3373	T3367	appos	Reagent,PER
R2109	T3374	T3375	punct	(,Pierce
R211	T461	T459	pobj	conditions,by
R2110	T3375	T3367	parataxis	Pierce,PER
R2111	T3376	T3375	punct	),Pierce
R2112	T3377	T3360	prep	according,extracted
R2113	T3378	T3377	prep	to,according
R2114	T3379	T3380	det	the,manufacturer
R2115	T3380	T3381	poss	manufacturer,instructions
R2116	T3381	T3378	pobj	instructions,to
R2117	T3382	T3380	case	's,manufacturer
R2118	T3383	T3360	punct	", ",extracted
R2119	T3384	T3360	advcl	using,extracted
R212	T462	T461	compound	stress,conditions
R2120	T3385	T3386	det	a,ratio
R2121	T3386	T3384	dobj	ratio,using
R2122	T3387	T3386	nummod	1,ratio
R2123	T3388	T3389	punct	:,10
R2124	T3389	T3387	prep	10,1
R2125	T3390	T3386	prep	of,ratio
R2126	T3391	T3392	compound	mouse,tissue
R2127	T3392	T3390	pobj	tissue,of
R2128	T3393	T3394	punct	(,mg
R2129	T3394	T3392	parataxis	mg,tissue
R213	T463	T457	cc	and,induced
R2130	T3395	T3394	punct	),mg
R2131	T3396	T3386	prep	to,ratio
R2132	T3397	T3398	compound	T,PER
R2133	T3398	T3400	compound	PER,reagent
R2134	T3399	T3398	punct	-,PER
R2135	T3400	T3396	pobj	reagent,to
R2136	T3401	T3402	punct	(,µl
R2137	T3402	T3400	parataxis	µl,reagent
R2138	T3403	T3402	punct	),µl
R2139	T3404	T3360	punct	.,extracted
R214	T464	T465	auxpass	is,overexpressed
R2140	T3406	T3407	nsubjpass	EMSA,performed
R2141	T3408	T3407	auxpass	was,performed
R2142	T3409	T3407	advmod	essentially,performed
R2143	T3410	T3411	mark	as,described
R2144	T3411	T3407	advcl	described,performed
R2145	T3412	T3411	advmod	previously,described
R2146	T3413	T3414	punct	(,10
R2147	T3414	T3411	parataxis	10,described
R2148	T3415	T3414	punct	),10
R2149	T3416	T3407	punct	.,performed
R215	T465	T457	conj	overexpressed,induced
R2150	T3418	T3419	det	All,reactions
R2151	T3419	T3421	nsubjpass	reactions,carried
R2152	T3420	T3419	compound	binding,reactions
R2153	T3422	T3421	auxpass	were,carried
R2154	T3423	T3421	prt	out,carried
R2155	T3424	T3421	prep	by,carried
R2156	T3425	T3424	pcomp	incubating,by
R2157	T3426	T3427	quantmod	2,5
R2158	T3427	T3429	nummod	5,fmol
R2159	T3428	T3427	punct	–,5
R216	T466	T465	prep	in,overexpressed
R2160	T3429	T3430	nmod	fmol,oligonucleotides
R2161	T3430	T3425	dobj	oligonucleotides,incubating
R2162	T3431	T3432	npadvmod	32P,labeled
R2163	T3432	T3430	amod	labeled,oligonucleotides
R2164	T3433	T3432	punct	-,labeled
R2165	T3434	T3432	npadvmod	end,labeled
R2166	T3435	T3432	punct	-,labeled
R2167	T3436	T3437	amod	double,stranded
R2168	T3437	T3430	amod	stranded,oligonucleotides
R2169	T3438	T3437	punct	-,stranded
R217	T467	T468	amod	many,cancers
R2170	T3439	T3425	prep	with,incubating
R2171	T3440	T3441	quantmod	100,130
R2172	T3441	T3443	nummod	130,µg
R2173	T3442	T3441	quantmod	to,130
R2174	T3443	T3444	compound	µg,extract
R2175	T3444	T3439	pobj	extract,with
R2176	T3445	T3446	compound	liver,protein
R2177	T3446	T3444	compound	protein,extract
R2178	T3447	T3421	punct	.,carried
R2179	T3449	T3450	prep	For,added
R218	T468	T466	pobj	cancers,in
R2180	T3451	T3452	compound	competition,experiments
R2181	T3452	T3449	pobj	experiments,For
R2182	T3453	T3450	punct	", ",added
R2183	T3454	T3455	nummod	5,pmol
R2184	T3455	T3450	nsubjpass	pmol,added
R2185	T3456	T3455	prep	of,pmol
R2186	T3457	T3458	amod	unlabeled,oligonucleotide
R2187	T3458	T3456	pobj	oligonucleotide,of
R2188	T3459	T3458	compound	competitor,oligonucleotide
R2189	T3460	T3450	auxpass	was,added
R219	T469	T434	punct	.,mediates
R2190	T3461	T3450	prep	to,added
R2191	T3462	T3463	det	the,reaction
R2192	T3463	T3461	pobj	reaction,to
R2193	T3464	T3463	compound	binding,reaction
R2194	T3465	T3450	prep	before,added
R2195	T3466	T3465	pobj	addition,before
R2196	T3467	T3466	prep	of,addition
R2197	T3468	T3469	det	the,extract
R2198	T3469	T3467	pobj	extract,of
R2199	T3470	T3450	punct	.,added
R22	T259	T260	npadvmod	Metal,responsive
R220	T471	T472	nsubjpass	MTF,involved
R2200	T3472	T3473	det	All,gels
R2201	T3473	T3475	nsubjpass	gels,developed
R2202	T3474	T3473	compound	EMSA,gels
R2203	T3476	T3475	auxpass	were,developed
R2204	T3477	T3475	advcl	using,developed
R2205	T3478	T3477	dobj	PhosphorImager,using
R2206	T3479	T3480	punct	(,Dynamics
R2207	T3480	T3478	parataxis	Dynamics,PhosphorImager
R2208	T3481	T3480	compound	Molecular,Dynamics
R2209	T3482	T3480	punct	),Dynamics
R221	T473	T471	punct	-,MTF
R2210	T3483	T3475	punct	.,developed
R2211	T3485	T3486	det	The,oligonucleotides
R2212	T3486	T3488	nsubjpass	oligonucleotides,annealed
R2213	T3487	T3486	amod	following,oligonucleotides
R2214	T3489	T3488	auxpass	were,annealed
R2215	T3490	T3488	cc	and,annealed
R2216	T3491	T3488	conj	used,annealed
R2217	T3492	T3491	prep	for,used
R2218	T3493	T3494	det	the,reactions
R2219	T3494	T3492	pobj	reactions,for
R222	T474	T471	nummod	1,MTF
R2220	T3495	T3491	punct	: ,used
R2221	T3496	T3497	compound	Csrp1,MRE1
R2222	T3497	T3491	dobj	MRE1,used
R2223	T3498	T3497	punct	: ,MRE1
R2224	T3499	T3500	nummod	5,GGAAACAAAACGGCGCGCACTCCGGCGC
R2225	T3500	T3497	appos	GGAAACAAAACGGCGCGCACTCCGGCGC,MRE1
R2226	T3501	T3499	punct	′,5
R2227	T3502	T3500	punct	-,GGAAACAAAACGGCGCGCACTCCGGCGC
R2228	T3503	T3500	punct	-,GGAAACAAAACGGCGCGCACTCCGGCGC
R2229	T3504	T3500	nummod	3,GGAAACAAAACGGCGCGCACTCCGGCGC
R223	T475	T472	auxpass	is,involved
R2230	T3505	T3500	punct	′,GGAAACAAAACGGCGCGCACTCCGGCGC
R2231	T3506	T3500	punct	;,GGAAACAAAACGGCGCGCACTCCGGCGC
R2232	T3507	T3508	nummod	5,CGGAGTGCGCGCCGTTTTGT
R2233	T3508	T3500	appos	CGGAGTGCGCGCCGTTTTGT,GGAAACAAAACGGCGCGCACTCCGGCGC
R2234	T3509	T3507	punct	′,5
R2235	T3510	T3508	punct	-,CGGAGTGCGCGCCGTTTTGT
R2236	T3511	T3508	compound	GGCTGCGC,CGGAGTGCGCGCCGTTTTGT
R2237	T3512	T3508	punct	-,CGGAGTGCGCGCCGTTTTGT
R2238	T3513	T3508	nummod	3,CGGAGTGCGCGCCGTTTTGT
R2239	T3514	T3508	punct	′,CGGAGTGCGCGCCGTTTTGT
R224	T476	T472	advmod	also,involved
R2240	T3515	T3497	punct	", ",MRE1
R2241	T3516	T3517	compound	Csrp1,MRE2
R2242	T3517	T3497	appos	MRE2,MRE1
R2243	T3518	T3517	punct	: ,MRE2
R2244	T3519	T3520	nummod	5,TGTTGTGGTGCAGTGTGCAAAGCCTAC
R2245	T3520	T3517	appos	TGTTGTGGTGCAGTGTGCAAAGCCTAC,MRE2
R2246	T3521	T3519	punct	′,5
R2247	T3522	T3520	punct	-,TGTTGTGGTGCAGTGTGCAAAGCCTAC
R2248	T3523	T3520	punct	-,TGTTGTGGTGCAGTGTGCAAAGCCTAC
R2249	T3524	T3520	nummod	3,TGTTGTGGTGCAGTGTGCAAAGCCTAC
R225	T477	T472	prep	in,involved
R2250	T3525	T3520	punct	′,TGTTGTGGTGCAGTGTGCAAAGCCTAC
R2251	T3526	T3520	punct	;,TGTTGTGGTGCAGTGTGCAAAGCCTAC
R2252	T3527	T3528	nummod	5,ACCAGTAGGCTTTGCACACTGCACCAC
R2253	T3528	T3520	appos	ACCAGTAGGCTTTGCACACTGCACCAC,TGTTGTGGTGCAGTGTGCAAAGCCTAC
R2254	T3529	T3527	punct	′,5
R2255	T3530	T3528	punct	-,ACCAGTAGGCTTTGCACACTGCACCAC
R2256	T3531	T3528	punct	-,ACCAGTAGGCTTTGCACACTGCACCAC
R2257	T3532	T3528	nummod	3,ACCAGTAGGCTTTGCACACTGCACCAC
R2258	T3533	T3528	punct	′,ACCAGTAGGCTTTGCACACTGCACCAC
R2259	T3534	T3497	punct	", ",MRE1
R226	T478	T479	det	the,response
R2260	T3535	T3536	compound	Csrp1,MRE3
R2261	T3536	T3497	appos	MRE3,MRE1
R2262	T3537	T3536	punct	: ,MRE3
R2263	T3538	T3539	nummod	5,GAGATCGCCATAGGGTGCAAAGAGAAG
R2264	T3539	T3536	appos	GAGATCGCCATAGGGTGCAAAGAGAAG,MRE3
R2265	T3540	T3538	punct	′,5
R2266	T3541	T3539	punct	-,GAGATCGCCATAGGGTGCAAAGAGAAG
R2267	T3542	T3539	punct	-,GAGATCGCCATAGGGTGCAAAGAGAAG
R2268	T3543	T3539	nummod	3,GAGATCGCCATAGGGTGCAAAGAGAAG
R2269	T3544	T3539	punct	′,GAGATCGCCATAGGGTGCAAAGAGAAG
R227	T479	T477	pobj	response,in
R2270	T3545	T3539	punct	;,GAGATCGCCATAGGGTGCAAAGAGAAG
R2271	T3546	T3547	nummod	5,GTGACTTCTCTTTGCACCCTATGGCGA
R2272	T3547	T3539	appos	GTGACTTCTCTTTGCACCCTATGGCGA,GAGATCGCCATAGGGTGCAAAGAGAAG
R2273	T3548	T3546	punct	′,5
R2274	T3549	T3547	punct	-,GTGACTTCTCTTTGCACCCTATGGCGA
R2275	T3550	T3547	punct	-,GTGACTTCTCTTTGCACCCTATGGCGA
R2276	T3551	T3547	nummod	3,GTGACTTCTCTTTGCACCCTATGGCGA
R2277	T3552	T3547	punct	′,GTGACTTCTCTTTGCACCCTATGGCGA
R2278	T3553	T3497	punct	", ",MRE1
R2279	T3554	T3555	compound	Csrp1,MRE4
R228	T480	T479	compound	cadmium,response
R2280	T3555	T3497	appos	MRE4,MRE1
R2281	T3556	T3555	punct	: ,MRE4
R2282	T3557	T3558	nummod	5,TGTCTTATTCTGGAGTGCAAGTTAGTC
R2283	T3558	T3555	appos	TGTCTTATTCTGGAGTGCAAGTTAGTC,MRE4
R2284	T3559	T3557	punct	′,5
R2285	T3560	T3558	punct	-,TGTCTTATTCTGGAGTGCAAGTTAGTC
R2286	T3561	T3558	punct	-,TGTCTTATTCTGGAGTGCAAGTTAGTC
R2287	T3562	T3558	nummod	3,TGTCTTATTCTGGAGTGCAAGTTAGTC
R2288	T3563	T3558	punct	′,TGTCTTATTCTGGAGTGCAAGTTAGTC
R2289	T3564	T3558	punct	;,TGTCTTATTCTGGAGTGCAAGTTAGTC
R229	T481	T479	prep	of,response
R2290	T3565	T3566	nummod	5,AGGGGACTAACTTGCACTCCAGAATAA
R2291	T3566	T3558	appos	AGGGGACTAACTTGCACTCCAGAATAA,TGTCTTATTCTGGAGTGCAAGTTAGTC
R2292	T3567	T3565	punct	′,5
R2293	T3568	T3566	punct	-,AGGGGACTAACTTGCACTCCAGAATAA
R2294	T3569	T3566	punct	-,AGGGGACTAACTTGCACTCCAGAATAA
R2295	T3570	T3566	nummod	3,AGGGGACTAACTTGCACTCCAGAATAA
R2296	T3571	T3566	punct	′,AGGGGACTAACTTGCACTCCAGAATAA
R2297	T3572	T3497	punct	", ",MRE1
R2298	T3573	T3497	appos	Gal4,MRE1
R2299	T3574	T3573	punct	: ,Gal4
R23	T260	T262	amod	responsive,factor
R230	T482	T483	npadvmod	cysteine,rich
R2300	T3575	T3576	nummod	5,TCCGGAGGACTGTCCTCCGG
R2301	T3576	T3573	appos	TCCGGAGGACTGTCCTCCGG,Gal4
R2302	T3577	T3575	punct	′,5
R2303	T3578	T3576	punct	-,TCCGGAGGACTGTCCTCCGG
R2304	T3579	T3576	punct	-,TCCGGAGGACTGTCCTCCGG
R2305	T3580	T3576	nummod	3,TCCGGAGGACTGTCCTCCGG
R2306	T3581	T3576	punct	′,TCCGGAGGACTGTCCTCCGG
R2307	T3582	T3576	punct	;,TCCGGAGGACTGTCCTCCGG
R2308	T3583	T3584	nummod	5,GCCGGAGGACAGTCCTCCGG
R2309	T3584	T3576	appos	GCCGGAGGACAGTCCTCCGG,TCCGGAGGACTGTCCTCCGG
R231	T483	T488	amod	rich,gene
R2310	T3585	T3583	punct	′,5
R2311	T3586	T3584	punct	-,GCCGGAGGACAGTCCTCCGG
R2312	T3587	T3584	punct	-,GCCGGAGGACAGTCCTCCGG
R2313	T3588	T3584	nummod	3,GCCGGAGGACAGTCCTCCGG
R2314	T3589	T3584	punct	′,GCCGGAGGACAGTCCTCCGG
R2315	T3590	T3497	punct	", ",MRE1
R2316	T3591	T3497	appos	MREd,MRE1
R2317	T3592	T3591	punct	[,MREd
R2318	T3593	T3591	appos	MRE,MREd
R2319	T3594	T3593	acl	derived,MRE
R232	T484	T482	punct	-,cysteine
R2320	T3595	T3594	prep	from,derived
R2321	T3596	T3597	compound	mouse,promoter
R2322	T3597	T3595	pobj	promoter,from
R2323	T3598	T3597	compound	Mt1,promoter
R2324	T3599	T3600	punct	(,10
R2325	T3600	T3594	parataxis	10,derived
R2326	T3601	T3600	punct	),10
R2327	T3602	T3591	punct	],MREd
R2328	T3603	T3591	punct	: ,MREd
R2329	T3604	T3605	nummod	5,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
R233	T485	T482	cc	and,cysteine
R2330	T3605	T3591	appos	CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG,MREd
R2331	T3606	T3604	punct	′,5
R2332	T3607	T3605	punct	-,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
R2333	T3608	T3605	punct	-,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
R2334	T3609	T3605	nummod	3,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
R2335	T3610	T3605	punct	′,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
R2336	T3611	T3605	punct	;,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
R2337	T3612	T3613	nummod	5,TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT
R2338	T3613	T3605	appos	TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT,CGAGGGAGCTCTGCACTCCGCCCGAAAAGTG
R2339	T3614	T3612	punct	′,5
R234	T486	T482	conj	glycine,cysteine
R2340	T3615	T3613	punct	-,TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT
R2341	T3616	T3613	punct	-,TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT
R2342	T3617	T3613	nummod	3,TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT
R2343	T3618	T3613	punct	′,TCGACACTTTTCGGGCGGAGTGCAGAGCTCCCTCGAGCT
R2344	T3619	T3497	punct	", ",MRE1
R2345	T3620	T3621	compound	MRE,s
R2346	T3621	T3497	appos	s,MRE1
R2347	T3622	T3621	punct	-,s
R2348	T3623	T3621	punct	[,s
R2349	T3624	T3625	amod	synthetic,sequence
R235	T487	T483	punct	-,rich
R2350	T3625	T3621	appos	sequence,s
R2351	T3626	T3625	compound	MRE,sequence
R2352	T3627	T3625	compound	consensus,sequence
R2353	T3628	T3629	punct	(,10
R2354	T3629	T3625	parataxis	10,sequence
R2355	T3630	T3629	punct	),10
R2356	T3631	T3621	punct	],s
R2357	T3632	T3621	punct	: ,s
R2358	T3633	T3634	nummod	5,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
R2359	T3634	T3621	appos	CGAGGGAGCTCTGCACACGGCCCGAAAAGTG,s
R236	T488	T481	pobj	gene,of
R2360	T3635	T3633	punct	′,5
R2361	T3636	T3634	punct	-,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
R2362	T3637	T3634	punct	-,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
R2363	T3638	T3634	nummod	3,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
R2364	T3639	T3634	punct	′,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
R2365	T3640	T3634	punct	;,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
R2366	T3641	T3642	nummod	5,TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT
R2367	T3642	T3634	appos	TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT,CGAGGGAGCTCTGCACACGGCCCGAAAAGTG
R2368	T3643	T3641	punct	′,5
R2369	T3644	T3642	punct	-,TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT
R237	T489	T488	nmod	protein,gene
R2370	T3645	T3642	punct	-,TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT
R2371	T3646	T3642	nummod	3,TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT
R2372	T3647	T3642	punct	′,TCGACACTTTTCGGGCCGTGTGCAGAGCTCCCTCGAGCT
R2373	T3648	T3497	punct	", ",MRE1
R2374	T3649	T3650	compound	Ndrg1,MRE1
R2375	T3650	T3497	appos	MRE1,MRE1
R2376	T3651	T3650	punct	: ,MRE1
R2377	T3652	T3653	nummod	5,CAGCCCAGGCAGGGTGCAGCACGAG
R2378	T3653	T3650	appos	CAGCCCAGGCAGGGTGCAGCACGAG,MRE1
R2379	T3654	T3652	punct	′,5
R238	T490	T489	nummod	1,protein
R2380	T3655	T3653	punct	-,CAGCCCAGGCAGGGTGCAGCACGAG
R2381	T3656	T3653	punct	-,CAGCCCAGGCAGGGTGCAGCACGAG
R2382	T3657	T3653	nummod	3,CAGCCCAGGCAGGGTGCAGCACGAG
R2383	T3658	T3653	punct	′,CAGCCCAGGCAGGGTGCAGCACGAG
R2384	T3659	T3653	punct	;,CAGCCCAGGCAGGGTGCAGCACGAG
R2385	T3660	T3661	nummod	5,CCGCCTCGTGCTGCACCCTGCCTGG
R2386	T3661	T3653	appos	CCGCCTCGTGCTGCACCCTGCCTGG,CAGCCCAGGCAGGGTGCAGCACGAG
R2387	T3662	T3660	punct	′,5
R2388	T3663	T3661	punct	-,CCGCCTCGTGCTGCACCCTGCCTGG
R2389	T3664	T3661	punct	-,CCGCCTCGTGCTGCACCCTGCCTGG
R239	T491	T488	punct	(,gene
R2390	T3665	T3661	nummod	3,CCGCCTCGTGCTGCACCCTGCCTGG
R2391	T3666	T3661	punct	′,CCGCCTCGTGCTGCACCCTGCCTGG
R2392	T3667	T3497	punct	", ",MRE1
R2393	T3668	T3669	compound	Ndrg1,MRE2
R2394	T3669	T3497	appos	MRE2,MRE1
R2395	T3670	T3669	punct	: ,MRE2
R2396	T3671	T3672	nummod	5,CACACGTTCGCTGCACACGCCGCGG
R2397	T3672	T3669	appos	CACACGTTCGCTGCACACGCCGCGG,MRE2
R2398	T3673	T3671	punct	′,5
R2399	T3674	T3672	punct	-,CACACGTTCGCTGCACACGCCGCGG
R24	T261	T260	punct	-,responsive
R240	T492	T488	appos	Csrp1,gene
R2400	T3675	T3672	punct	-,CACACGTTCGCTGCACACGCCGCGG
R2401	T3676	T3672	nummod	3,CACACGTTCGCTGCACACGCCGCGG
R2402	T3677	T3672	punct	′,CACACGTTCGCTGCACACGCCGCGG
R2403	T3678	T3672	punct	;,CACACGTTCGCTGCACACGCCGCGG
R2404	T3679	T3680	nummod	5,GGGACCGCGGCGTGTGCAGCGAACG
R2405	T3680	T3672	appos	GGGACCGCGGCGTGTGCAGCGAACG,CACACGTTCGCTGCACACGCCGCGG
R2406	T3681	T3679	punct	′,5
R2407	T3682	T3680	punct	-,GGGACCGCGGCGTGTGCAGCGAACG
R2408	T3683	T3680	punct	-,GGGACCGCGGCGTGTGCAGCGAACG
R2409	T3684	T3680	nummod	3,GGGACCGCGGCGTGTGCAGCGAACG
R241	T493	T488	punct	),gene
R2410	T3685	T3680	punct	′,GGGACCGCGGCGTGTGCAGCGAACG
R2411	T3686	T3497	punct	", ",MRE1
R2412	T3687	T3688	compound	Ndrg1,MRE3
R2413	T3688	T3497	appos	MRE3,MRE1
R2414	T3689	T3688	punct	",",MRE3
R2415	T3690	T3688	nummod	4,MRE3
R2416	T3691	T3688	punct	: ,MRE3
R2417	T3692	T3693	nummod	5,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
R2418	T3693	T3688	appos	GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC,MRE3
R2419	T3694	T3692	punct	′,5
R242	T494	T488	punct	", ",gene
R2420	T3695	T3693	punct	-,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
R2421	T3696	T3693	punct	-,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
R2422	T3697	T3693	nummod	3,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
R2423	T3698	T3693	punct	′,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
R2424	T3699	T3693	punct	;,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
R2425	T3700	T3701	nummod	5,TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC
R2426	T3701	T3693	appos	TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC,GGAGTCCTTATGCACACGCGCACGAGCGCGCACGGGCAC
R2427	T3702	T3700	punct	′,5
R2428	T3703	T3701	punct	-,TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC
R2429	T3704	T3701	punct	-,TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC
R243	T495	T496	dep	which,implicated
R2430	T3705	T3701	nummod	3,TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC
R2431	T3706	T3701	punct	′,TGGTGTGCCCGTGCGCGCTCGTGCGCGTGTGCATAAGGAC
R2432	T3707	T3497	punct	", ",MRE1
R2433	T3708	T3709	compound	Sepw1,MRE1
R2434	T3709	T3497	appos	MRE1,MRE1
R2435	T3710	T3709	punct	: ,MRE1
R2436	T3711	T3712	nummod	5,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
R2437	T3712	T3709	appos	GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC,MRE1
R2438	T3713	T3711	punct	′,5
R2439	T3714	T3712	punct	-,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
R244	T496	T488	relcl	implicated,gene
R2440	T3715	T3712	punct	-,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
R2441	T3716	T3712	nummod	3,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
R2442	T3717	T3712	punct	′,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
R2443	T3718	T3712	punct	;,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
R2444	T3719	T3720	nummod	5,CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC
R2445	T3720	T3712	appos	CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC,GAGGCAGTCGGCTGTGCGCACGGCCCCACGCTC
R2446	T3721	T3719	punct	′,5
R2447	T3722	T3720	punct	-,CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC
R2448	T3723	T3720	punct	-,CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC
R2449	T3724	T3720	nummod	3,CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC
R245	T497	T496	auxpass	is,implicated
R2450	T3725	T3720	punct	′,CTCTGAGCGTGGGGCCGTGCGCACAGCCGACTGC
R2451	T3726	T3497	punct	", ",MRE1
R2452	T3727	T3728	compound	Sepw1,MRE2
R2453	T3728	T3497	appos	MRE2,MRE1
R2454	T3729	T3728	punct	: ,MRE2
R2455	T3730	T3731	nummod	5,ATGGTTTTGGGGGTGCGCAGGGGGTCTG
R2456	T3731	T3728	appos	ATGGTTTTGGGGGTGCGCAGGGGGTCTG,MRE2
R2457	T3732	T3730	punct	′,5
R2458	T3733	T3731	punct	-,ATGGTTTTGGGGGTGCGCAGGGGGTCTG
R2459	T3734	T3731	punct	-,ATGGTTTTGGGGGTGCGCAGGGGGTCTG
R246	T498	T496	prep	in,implicated
R2460	T3735	T3731	nummod	3,ATGGTTTTGGGGGTGCGCAGGGGGTCTG
R2461	T3736	T3731	punct	′,ATGGTTTTGGGGGTGCGCAGGGGGTCTG
R2462	T3737	T3731	punct	;,ATGGTTTTGGGGGTGCGCAGGGGGTCTG
R2463	T3738	T3739	nummod	5,CGACAGACCCCCTGCGCACCCCCAAAAC
R2464	T3739	T3731	appos	CGACAGACCCCCTGCGCACCCCCAAAAC,ATGGTTTTGGGGGTGCGCAGGGGGTCTG
R2465	T3740	T3738	punct	′,5
R2466	T3741	T3739	punct	-,CGACAGACCCCCTGCGCACCCCCAAAAC
R2467	T3742	T3739	punct	-,CGACAGACCCCCTGCGCACCCCCAAAAC
R2468	T3743	T3739	nummod	3,CGACAGACCCCCTGCGCACCCCCAAAAC
R2469	T3744	T3739	punct	′,CGACAGACCCCCTGCGCACCCCCAAAAC
R247	T499	T500	amod	cytoskeletal,organization
R2470	T3745	T3497	punct	", ",MRE1
R2471	T3746	T3747	compound	Slc39a10,MRE1
R2472	T3747	T3497	appos	MRE1,MRE1
R2473	T3748	T3747	punct	: ,MRE1
R2474	T3749	T3750	nummod	5,GAATACACGACTGGGTGCAGCCGGGGTTTGG
R2475	T3750	T3747	appos	GAATACACGACTGGGTGCAGCCGGGGTTTGG,MRE1
R2476	T3751	T3749	punct	′,5
R2477	T3752	T3750	punct	-,GAATACACGACTGGGTGCAGCCGGGGTTTGG
R2478	T3753	T3750	punct	-,GAATACACGACTGGGTGCAGCCGGGGTTTGG
R2479	T3754	T3750	nummod	3,GAATACACGACTGGGTGCAGCCGGGGTTTGG
R248	T500	T498	pobj	organization,in
R2480	T3755	T3750	punct	′,GAATACACGACTGGGTGCAGCCGGGGTTTGG
R2481	T3756	T3750	punct	;,GAATACACGACTGGGTGCAGCCGGGGTTTGG
R2482	T3757	T3758	nummod	5,GGTACCAAACCCCGGCTGCACCCAGTCGTGTA
R2483	T3758	T3750	appos	GGTACCAAACCCCGGCTGCACCCAGTCGTGTA,GAATACACGACTGGGTGCAGCCGGGGTTTGG
R2484	T3759	T3757	punct	′,5
R2485	T3760	T3758	punct	-,GGTACCAAACCCCGGCTGCACCCAGTCGTGTA
R2486	T3761	T3758	punct	-,GGTACCAAACCCCGGCTGCACCCAGTCGTGTA
R2487	T3762	T3758	nummod	3,GGTACCAAACCCCGGCTGCACCCAGTCGTGTA
R2488	T3763	T3758	punct	′,GGTACCAAACCCCGGCTGCACCCAGTCGTGTA
R2489	T3764	T3497	punct	", ",MRE1
R249	T501	T472	punct	.,involved
R2490	T3765	T3766	compound	Slc39a10,MRE2
R2491	T3766	T3497	appos	MRE2,MRE1
R2492	T3767	T3766	punct	: ,MRE2
R2493	T3768	T3769	nummod	5,GCGGAGAGGAGATGCACACGGCACTCG
R2494	T3769	T3766	appos	GCGGAGAGGAGATGCACACGGCACTCG,MRE2
R2495	T3770	T3768	punct	′,5
R2496	T3771	T3769	punct	-,GCGGAGAGGAGATGCACACGGCACTCG
R2497	T3772	T3769	punct	-,GCGGAGAGGAGATGCACACGGCACTCG
R2498	T3773	T3769	nummod	3,GCGGAGAGGAGATGCACACGGCACTCG
R2499	T3774	T3769	punct	′,GCGGAGAGGAGATGCACACGGCACTCG
R25	T262	T264	nsubj	factor,regulates
R250	T503	T504	prep	In,represses
R2500	T3775	T3769	punct	;,GCGGAGAGGAGATGCACACGGCACTCG
R2501	T3776	T3777	nummod	5,CACTCGAGTGCCGTGTGCATCTCCTCT
R2502	T3777	T3769	appos	CACTCGAGTGCCGTGTGCATCTCCTCT,GCGGAGAGGAGATGCACACGGCACTCG
R2503	T3778	T3776	punct	′,5
R2504	T3779	T3777	punct	-,CACTCGAGTGCCGTGTGCATCTCCTCT
R2505	T3780	T3777	punct	-,CACTCGAGTGCCGTGTGCATCTCCTCT
R2506	T3781	T3777	nummod	3,CACTCGAGTGCCGTGTGCATCTCCTCT
R2507	T3782	T3777	punct	′,CACTCGAGTGCCGTGTGCATCTCCTCT
R2508	T3783	T3497	punct	", ",MRE1
R2509	T3784	T3785	nmod	Specificity,protein
R251	T505	T503	pobj	contrast,In
R2510	T3785	T3786	nmod	protein,sequence
R2511	T3786	T3497	appos	sequence,MRE1
R2512	T3787	T3785	nummod	1,protein
R2513	T3788	T3785	punct	(,protein
R2514	T3789	T3785	appos	Sp1,protein
R2515	T3790	T3786	punct	),sequence
R2516	T3791	T3786	compound	binding,sequence
R2517	T3792	T3793	punct	(,10
R2518	T3793	T3786	parataxis	10,sequence
R2519	T3794	T3793	punct	),10
R252	T506	T504	punct	", ",represses
R2520	T3795	T3497	punct	: ,MRE1
R2521	T3796	T3797	nummod	5,CGAGGCCCCGCCCAG
R2522	T3797	T3497	appos	CGAGGCCCCGCCCAG,MRE1
R2523	T3798	T3796	punct	′,5
R2524	T3799	T3797	punct	-,CGAGGCCCCGCCCAG
R2525	T3800	T3797	punct	-,CGAGGCCCCGCCCAG
R2526	T3801	T3797	nummod	3,CGAGGCCCCGCCCAG
R2527	T3802	T3797	punct	′,CGAGGCCCCGCCCAG
R2528	T3803	T3797	punct	;,CGAGGCCCCGCCCAG
R2529	T3804	T3805	nummod	5,TCGACTGGGCGGGGCCTCGAGct
R253	T507	T504	nsubj	MTF,represses
R2530	T3805	T3797	appos	TCGACTGGGCGGGGCCTCGAGct,CGAGGCCCCGCCCAG
R2531	T3806	T3804	punct	′,5
R2532	T3807	T3805	punct	-,TCGACTGGGCGGGGCCTCGAGct
R2533	T3808	T3805	punct	-,TCGACTGGGCGGGGCCTCGAGct
R2534	T3809	T3805	nummod	3,TCGACTGGGCGGGGCCTCGAGct
R2535	T3810	T3805	punct	′,TCGACTGGGCGGGGCCTCGAGct
R2536	T3811	T3488	punct	.,annealed
R2537	T3873	T3888	compound	Mtf1loxP,embryo
R2538	T3874	T3898	conj	streptomycin,serum
R2539	T3875	T3876	compound	Cell,culture
R254	T508	T507	punct	-,MTF
R2540	T3878	T3879	amod	Primary,fibroblasts
R2541	T3879	T3881	nsubjpass	fibroblasts,isolated
R2542	T3880	T3879	amod	embryonic,fibroblasts
R2543	T3882	T3881	auxpass	were,isolated
R2544	T3883	T3881	prep	from,isolated
R2545	T3884	T3885	det	a,day
R2546	T3885	T3887	npadvmod	day,old
R2547	T3886	T3885	nummod	12.5,day
R2548	T3887	T3888	amod	old,embryo
R2549	T3888	T3883	pobj	embryo,from
R255	T509	T507	nummod	1,MTF
R2550	T3889	T3888	compound	mouse,embryo
R2551	T3890	T3881	cc	and,isolated
R2552	T3891	T3881	conj	grown,isolated
R2553	T3892	T3891	prep	in,grown
R2554	T3893	T3892	pobj	DMEM,in
R2555	T3894	T3893	acl	supplemented,DMEM
R2556	T3895	T3894	prep	with,supplemented
R2557	T3896	T3897	nummod	10,%
R2558	T3897	T3898	nmod	%,serum
R2559	T3898	T3895	pobj	serum,with
R256	T510	T511	det	the,expression
R2560	T3899	T3898	amod	fetal,serum
R2561	T3900	T3898	amod	bovine,serum
R2562	T3901	T3902	punct	(,ICN
R2563	T3902	T3898	parataxis	ICN,serum
R2564	T3903	T3902	punct	),ICN
R2565	T3904	T3898	punct	", ",serum
R2566	T3905	T3906	nummod	100,U
R2567	T3906	T3874	nmod	U,streptomycin
R2568	T3907	T3908	punct	/,ml
R2569	T3908	T3906	prep	ml,U
R257	T511	T504	dobj	expression,represses
R2570	T3909	T3874	compound	penicillin,streptomycin
R2571	T3910	T3874	punct	–,streptomycin
R2572	T3911	T3912	punct	(,BRL
R2573	T3912	T3874	parataxis	BRL,streptomycin
R2574	T3913	T3912	compound	Gibco,BRL
R2575	T3914	T3912	punct	),BRL
R2576	T3915	T3874	cc	and,streptomycin
R2577	T3916	T3917	nummod	2,mM
R2578	T3917	T3918	compound	mM,glutamine
R2579	T3918	T3874	conj	glutamine,streptomycin
R258	T512	T511	amod	basal,expression
R2580	T3919	T3918	compound	l,glutamine
R2581	T3920	T3918	punct	-,glutamine
R2582	T3921	T3922	punct	(,BRL
R2583	T3922	T3918	parataxis	BRL,glutamine
R2584	T3923	T3922	compound	Gibco,BRL
R2585	T3924	T3922	punct	),BRL
R2586	T3925	T3881	punct	.,isolated
R2587	T3927	T3928	nummod	100,mm
R2588	T3928	T3929	compound	mm,plates
R2589	T3929	T3930	nsubjpass	plates,transfected
R259	T513	T511	prep	of,expression
R2590	T3931	T3929	prep	with,plates
R2591	T3932	T3933	amod	primary,cells
R2592	T3933	T3931	pobj	cells,with
R2593	T3934	T3930	auxpass	were,transfected
R2594	T3935	T3930	prep	with,transfected
R2595	T3936	T3937	nummod	10,µg
R2596	T3937	T3935	pobj	µg,with
R2597	T3938	T3937	prep	of,µg
R2598	T3939	T3940	det	an,plasmid
R2599	T3940	T3938	pobj	plasmid,of
R26	T263	T262	compound	transcription,factor
R260	T514	T513	pobj	Slc39a10,of
R2600	T3941	T3940	compound	expression,plasmid
R2601	T3942	T3940	acl	coding,plasmid
R2602	T3943	T3942	prep	for,coding
R2603	T3944	T3945	amod	simian,virus
R2604	T3945	T3946	nmod	virus,antigen
R2605	T3946	T3943	pobj	antigen,for
R2606	T3947	T3945	nummod	40,virus
R2607	T3948	T3945	punct	(,virus
R2608	T3949	T3945	appos	SV40,virus
R2609	T3950	T3946	punct	),antigen
R261	T515	T514	punct	", ",Slc39a10
R2610	T3951	T3952	amod	large,T
R2611	T3952	T3946	compound	T,antigen
R2612	T3953	T3946	acl	driven,antigen
R2613	T3954	T3953	agent	by,driven
R2614	T3955	T3956	det	the,promoter
R2615	T3956	T3954	pobj	promoter,by
R2616	T3957	T3956	nmod	cytomegalovirus,promoter
R2617	T3958	T3957	punct	(,cytomegalovirus
R2618	T3959	T3957	appos	CMV,cytomegalovirus
R2619	T3960	T3956	punct	),promoter
R262	T516	T517	det	a,transporter
R2620	T3961	T3930	punct	", ",transfected
R2621	T3962	T3930	advcl	using,transfected
R2622	T3963	T3964	nmod	lipofectamine,reagent
R2623	T3964	T3962	dobj	reagent,using
R2624	T3965	T3963	punct	™,lipofectamine
R2625	T3966	T3967	punct	(,Invitrogen
R2626	T3967	T3964	parataxis	Invitrogen,reagent
R2627	T3968	T3967	punct	),Invitrogen
R2628	T3969	T3962	prep	according,using
R2629	T3970	T3969	prep	to,according
R263	T517	T514	appos	transporter,Slc39a10
R2630	T3971	T3972	det	the,manufacturer
R2631	T3972	T3973	poss	manufacturer,instructions
R2632	T3973	T3970	pobj	instructions,to
R2633	T3974	T3972	case	's,manufacturer
R2634	T3975	T3930	punct	.,transfected
R2635	T3977	T3978	compound	Cell,foci
R2636	T3978	T3979	nsubjpass	foci,isolated
R2637	T3980	T3979	auxpass	were,isolated
R2638	T3981	T3979	cc	and,isolated
R2639	T3982	T3983	det	the,line
R264	T518	T517	amod	putative,transporter
R2640	T3983	T3989	nsubjpass	line,derived
R2641	T3984	T3983	amod	immortalized,line
R2642	T3985	T3986	nmod	mouse,fibroblast
R2643	T3986	T3983	compound	fibroblast,line
R2644	T3987	T3986	amod	embryonic,fibroblast
R2645	T3988	T3983	compound	cell,line
R2646	T3989	T3979	conj	derived,isolated
R2647	T3990	T3983	appos	ckoC,line
R2648	T3991	T3989	auxpass	was,derived
R2649	T3992	T3989	prep	from,derived
R265	T519	T517	compound	zinc,transporter
R2650	T3993	T3992	pobj	one,from
R2651	T3994	T3993	prep	of,one
R2652	T3995	T3994	pobj	them,of
R2653	T3996	T3989	punct	.,derived
R2654	T3998	T3999	det	The,genotype
R2655	T3999	T4001	nsubjpass	genotype,confirmed
R2656	T4000	T3999	compound	Mtf1loxP,genotype
R2657	T4002	T3999	prep	of,genotype
R2658	T4003	T4004	det	this,line
R2659	T4004	T4002	pobj	line,of
R266	T520	T504	punct	.,represses
R2660	T4005	T4006	advmod	as,as
R2661	T4006	T3999	cc	as,genotype
R2662	T4007	T4006	advmod	well,as
R2663	T4008	T4009	det	the,integration
R2664	T4009	T3999	conj	integration,genotype
R2665	T4010	T4009	amod	genomic,integration
R2666	T4011	T4009	prep	of,integration
R2667	T4012	T4013	det	the,antigen
R2668	T4013	T4011	pobj	antigen,of
R2669	T4014	T4013	compound	T,antigen
R267	T522	T523	prep	In,induced
R2670	T4015	T4001	auxpass	were,confirmed
R2671	T4016	T4001	agent	by,confirmed
R2672	T4017	T4016	pobj	PCR,by
R2673	T4018	T4001	punct	.,confirmed
R2674	T4020	T4021	nummod	100,mm
R2675	T4021	T4022	compound	mm,plates
R2676	T4022	T4023	nsubjpass	plates,transfected
R2677	T4024	T4022	prep	with,plates
R2678	T4025	T4026	det	these,cells
R2679	T4026	T4024	pobj	cells,with
R268	T524	T525	det	a,pathway
R2680	T4027	T4023	auxpass	were,transfected
R2681	T4028	T4023	advmod	further,transfected
R2682	T4029	T4023	agent	by,transfected
R2683	T4030	T4031	det	the,method
R2684	T4031	T4029	pobj	method,by
R2685	T4032	T4033	compound	calcium,phosphate
R2686	T4033	T4031	compound	phosphate,method
R2687	T4034	T4035	punct	(,30
R2688	T4035	T4031	parataxis	30,method
R2689	T4036	T4035	punct	),30
R269	T525	T522	pobj	pathway,In
R2690	T4037	T4031	prep	with,method
R2691	T4038	T4039	nummod	19.6,µg
R2692	T4039	T4037	pobj	µg,with
R2693	T4040	T4039	prep	of,µg
R2694	T4041	T4042	det	an,plasmid
R2695	T4042	T4040	pobj	plasmid,of
R2696	T4043	T4042	compound	expression,plasmid
R2697	T4044	T4042	prep	for,plasmid
R2698	T4045	T4046	compound	Cre,recombinase
R2699	T4046	T4044	pobj	recombinase,for
R27	T265	T262	nummod	1,factor
R270	T526	T525	amod	independent,pathway
R2700	T4047	T4042	acl	driven,plasmid
R2701	T4048	T4047	agent	by,driven
R2702	T4049	T4050	det	the,promoter
R2703	T4050	T4048	pobj	promoter,by
R2704	T4051	T4050	compound	CMV,promoter
R2705	T4052	T4039	cc	and,µg
R2706	T4053	T4054	nummod	0.4,µg
R2707	T4054	T4039	conj	µg,µg
R2708	T4055	T4054	prep	of,µg
R2709	T4056	T4057	det	an,plasmid
R271	T527	T526	prep	of,independent
R2710	T4057	T4055	pobj	plasmid,of
R2711	T4058	T4057	compound	expression,plasmid
R2712	T4059	T4057	prep	for,plasmid
R2713	T4060	T4061	det	the,gene
R2714	T4061	T4059	pobj	gene,for
R2715	T4062	T4061	compound	neomycin,gene
R2716	T4063	T4061	compound	resistance,gene
R2717	T4064	T4061	prep	under,gene
R2718	T4065	T4066	det	the,control
R2719	T4066	T4064	pobj	control,under
R272	T528	T527	pobj	MTF,of
R2720	T4067	T4066	prep	of,control
R2721	T4068	T4069	det	the,promoter
R2722	T4069	T4067	pobj	promoter,of
R2723	T4070	T4069	compound	TK,promoter
R2724	T4071	T4023	punct	.,transfected
R2725	T4073	T4074	advmod	Stably,transfected
R2726	T4074	T4075	amod	transfected,cells
R2727	T4075	T4076	nsubjpass	cells,selected
R2728	T4077	T4076	auxpass	were,selected
R2729	T4078	T4076	prep	in,selected
R273	T529	T528	punct	-,MTF
R2730	T4079	T4080	det	the,presence
R2731	T4080	T4078	pobj	presence,in
R2732	T4081	T4080	prep	of,presence
R2733	T4082	T4083	nummod	0.4,µg
R2734	T4083	T4084	nmod	µg,G418
R2735	T4084	T4081	pobj	G418,of
R2736	T4085	T4086	punct	/,µl
R2737	T4086	T4083	prep	µl,µg
R2738	T4087	T4088	punct	(,Calbiochem
R2739	T4088	T4084	parataxis	Calbiochem,G418
R274	T530	T528	nummod	1,MTF
R2740	T4089	T4088	punct	),Calbiochem
R2741	T4090	T4076	punct	", ",selected
R2742	T4091	T4092	amod	isolated,clones
R2743	T4092	T4093	nsubjpass	clones,harvested
R2744	T4093	T4076	conj	harvested,selected
R2745	T4094	T4092	prep	of,clones
R2746	T4095	T4096	amod	resistant,cells
R2747	T4096	T4094	pobj	cells,of
R2748	T4097	T4093	auxpass	were,harvested
R2749	T4098	T4093	cc	and,harvested
R275	T531	T523	punct	", ",induced
R2750	T4099	T4093	conj	grown,harvested
R2751	T4100	T4099	advmod	independently,grown
R2752	T4101	T4093	punct	", ",harvested
R2753	T4102	T4093	cc	and,harvested
R2754	T4103	T4104	det	the,expression
R2755	T4104	T4105	nsubjpass	expression,analyzed
R2756	T4105	T4093	conj	analyzed,harvested
R2757	T4106	T4104	prep	of,expression
R2758	T4107	T4108	compound	Cre,recombinase
R2759	T4108	T4106	pobj	recombinase,of
R276	T532	T523	nsubj	cadmium,induced
R2760	T4109	T4104	cc	and,expression
R2761	T4110	T4104	conj	excision,expression
R2762	T4111	T4110	prep	of,excision
R2763	T4112	T4113	nmod	exons,3
R2764	T4113	T4111	pobj	3,of
R2765	T4114	T4113	cc	and,3
R2766	T4115	T4113	conj	4,3
R2767	T4116	T4113	prep	of,3
R2768	T4117	T4116	pobj	Mtf1,of
R2769	T4118	T4105	auxpass	were,analyzed
R277	T533	T523	advmod	also,induced
R2770	T4119	T4105	agent	by,analyzed
R2771	T4120	T4121	compound	RT,PCR
R2772	T4121	T4119	pobj	PCR,by
R2773	T4122	T4121	punct	–,PCR
R2774	T4123	T4105	punct	.,analyzed
R2775	T4125	T4126	det	The,lines
R2776	T4126	T4128	nsubjpass	lines,chosen
R2777	T4127	T4126	compound	cell,lines
R2778	T4129	T4126	appos	delC19,lines
R2779	T4130	T4129	punct	", ",delC19
R278	T534	T535	det	the,transcription
R2780	T4131	T4129	conj	delC21,delC19
R2781	T4132	T4131	cc	and,delC21
R2782	T4133	T4131	conj	delC23,delC21
R2783	T4134	T4126	prep	with,lines
R2784	T4135	T4136	det	a,deletion
R2785	T4136	T4134	pobj	deletion,with
R2786	T4137	T4136	prep	of,deletion
R2787	T4138	T4137	pobj	Mtf1,of
R2788	T4139	T4128	auxpass	were,chosen
R2789	T4140	T4128	prep	for,chosen
R279	T535	T523	dobj	transcription,induced
R2790	T4141	T4142	amod	further,experiments
R2791	T4142	T4140	pobj	experiments,for
R2792	T4143	T4128	punct	.,chosen
R2795	T4279	T4280	compound	Cytotoxicity,assay
R2796	T4282	T4283	nsubjpass	Samples,plated
R2797	T4284	T4282	prep	of,Samples
R2798	T4285	T4286	quantmod	1,104
R2799	T4286	T4288	nummod	104,cells
R28	T266	T262	punct	(,factor
R280	T536	T535	prep	of,transcription
R2800	T4287	T4286	punct	×,104
R2801	T4288	T4284	pobj	cells,of
R2802	T4289	T4290	punct	/,well
R2803	T4290	T4288	prep	well,cells
R2804	T4291	T4283	auxpass	were,plated
R2805	T4292	T4283	prep	in,plated
R2806	T4293	T4294	nummod	96,well
R2807	T4294	T4296	compound	well,plates
R2808	T4295	T4294	punct	-,well
R2809	T4296	T4292	pobj	plates,in
R281	T537	T536	pobj	genes,of
R2810	T4297	T4298	compound	tissue,culture
R2811	T4298	T4296	compound	culture,plates
R2812	T4299	T4283	cc	and,plated
R2813	T4300	T4283	conj	allowed,plated
R2814	T4301	T4302	aux	to,adhere
R2815	T4302	T4300	xcomp	adhere,allowed
R2816	T4303	T4302	prep	for,adhere
R2817	T4304	T4305	nummod	24,h
R2818	T4305	T4303	pobj	h,for
R2819	T4306	T4283	punct	.,plated
R282	T538	T537	acl	involved,genes
R2820	T4308	T4309	det	The,cells
R2821	T4309	T4310	nsubjpass	cells,pre-incubated
R2822	T4311	T4310	auxpass	were,pre-incubated
R2823	T4312	T4310	advmod	then,pre-incubated
R2824	T4313	T4310	prep	for,pre-incubated
R2825	T4314	T4315	nummod	24,h
R2826	T4315	T4313	pobj	h,for
R2827	T4316	T4310	prep	in,pre-incubated
R2828	T4317	T4316	pobj	medium,in
R2829	T4318	T4317	acl	containing,medium
R283	T539	T538	prep	in,involved
R2830	T4319	T4320	nummod	0,µM
R2831	T4320	T4318	dobj	µM,containing
R2832	T4321	T4319	punct	", ",0
R2833	T4322	T4319	conj	5,0
R2834	T4323	T4322	punct	", ",5
R2835	T4324	T4322	conj	10,5
R2836	T4325	T4324	punct	", ",10
R2837	T4326	T4324	conj	25,10
R2838	T4327	T4326	cc	or,25
R2839	T4328	T4326	conj	50,25
R284	T540	T541	det	the,synthesis
R2840	T4329	T4330	nmod	l,sulfoximine
R2841	T4330	T4320	appos	sulfoximine,µM
R2842	T4331	T4330	punct	-,sulfoximine
R2843	T4332	T4330	nmod	buthionine,sulfoximine
R2844	T4333	T4330	punct	-,sulfoximine
R2845	T4334	T4330	punct	[,sulfoximine
R2846	T4335	T4330	nmod	S,sulfoximine
R2847	T4336	T4330	punct	",",sulfoximine
R2848	T4337	T4330	nmod	R,sulfoximine
R2849	T4338	T4330	punct	],sulfoximine
R285	T541	T539	pobj	synthesis,in
R2850	T4339	T4330	punct	-,sulfoximine
R2851	T4340	T4330	punct	(,sulfoximine
R2852	T4341	T4330	appos	BSO,sulfoximine
R2853	T4342	T4320	punct	),µM
R2854	T4343	T4344	punct	(,Sigma
R2855	T4344	T4320	parataxis	Sigma,µM
R2856	T4345	T4344	punct	),Sigma
R2857	T4346	T4320	punct	", ",µM
R2858	T4347	T4348	det	a,drug
R2859	T4348	T4320	appos	drug,µM
R286	T542	T541	cc	and,synthesis
R2860	T4349	T4350	dep	that,inhibits
R2861	T4350	T4348	relcl	inhibits,drug
R2862	T4351	T4352	compound	glutathione,synthesis
R2863	T4352	T4350	dobj	synthesis,inhibits
R2864	T4353	T4354	punct	(,31
R2865	T4354	T4350	parataxis	31,inhibits
R2866	T4355	T4354	punct	),31
R2867	T4356	T4310	punct	.,pre-incubated
R2868	T4358	T4359	advmod	Later,exposed
R2869	T4360	T4359	punct	", ",exposed
R287	T543	T541	conj	regeneration,synthesis
R2870	T4361	T4359	nsubjpass	cells,exposed
R2871	T4362	T4359	auxpass	were,exposed
R2872	T4363	T4359	prep	to,exposed
R2873	T4364	T4365	nummod	0,µM
R2874	T4365	T4372	compound	µM,CdCl2
R2875	T4366	T4364	punct	", ",0
R2876	T4367	T4364	conj	5,0
R2877	T4368	T4367	punct	", ",5
R2878	T4369	T4367	conj	10,5
R2879	T4370	T4369	cc	or,10
R288	T544	T541	prep	of,synthesis
R2880	T4371	T4369	conj	20,10
R2881	T4372	T4363	pobj	CdCl2,to
R2882	T4373	T4359	prep	in,exposed
R2883	T4374	T4375	det	the,medium
R2884	T4375	T4373	pobj	medium,in
R2885	T4376	T4375	amod	specified,medium
R2886	T4377	T4375	compound	pre-incubation,medium
R2887	T4378	T4359	prep	for,exposed
R2888	T4379	T4380	det	an,h
R2889	T4380	T4378	pobj	h,for
R289	T545	T544	pobj	glutathione,of
R2890	T4381	T4380	amod	additional,h
R2891	T4382	T4380	nummod	24,h
R2892	T4383	T4359	punct	.,exposed
R2893	T4385	T4386	nsubjpass	Cytotoxicity,determined
R2894	T4387	T4386	auxpass	was,determined
R2895	T4388	T4386	agent	by,determined
R2896	T4389	T4390	det	the,Kit
R2897	T4390	T4388	pobj	Kit,by
R2898	T4391	T4392	npadvmod	MTT,based
R2899	T4392	T4390	amod	based,Kit
R29	T267	T262	appos	MTF,factor
R290	T546	T545	punct	", ",glutathione
R2900	T4393	T4394	punct	(,bromid
R2901	T4394	T4391	parataxis	bromid,MTT
R2902	T4395	T4396	nummod	3,dimethylthiazol
R2903	T4396	T4394	nmod	dimethylthiazol,bromid
R2904	T4397	T4396	punct	-,dimethylthiazol
R2905	T4398	T4396	punct	[,dimethylthiazol
R2906	T4399	T4396	nummod	4,dimethylthiazol
R2907	T4400	T4396	punct	",",dimethylthiazol
R2908	T4401	T4396	nummod	5,dimethylthiazol
R2909	T4402	T4396	punct	-,dimethylthiazol
R291	T547	T548	det	a,antioxidant
R2910	T4403	T4396	punct	-,dimethylthiazol
R2911	T4404	T4405	nummod	2,yl
R2912	T4405	T4396	appos	yl,dimethylthiazol
R2913	T4406	T4405	punct	-,yl
R2914	T4407	T4396	punct	],dimethylthiazol
R2915	T4408	T4396	punct	-,dimethylthiazol
R2916	T4409	T4396	nummod	2,dimethylthiazol
R2917	T4410	T4394	punct	",",bromid
R2918	T4411	T4412	nummod	5,diphenyl
R2919	T4412	T4394	compound	diphenyl,bromid
R292	T548	T545	appos	antioxidant,glutathione
R2920	T4413	T4412	punct	-,diphenyl
R2921	T4414	T4394	compound	tetrazolium,bromid
R2922	T4415	T4394	punct	),bromid
R2923	T4416	T4392	punct	-,based
R2924	T4417	T4390	compound	Cell,Kit
R2925	T4418	T4390	compound	Proliferation,Kit
R2926	T4419	T4390	nummod	I,Kit
R2927	T4420	T4421	punct	(,Roche
R2928	T4421	T4390	parataxis	Roche,Kit
R2929	T4422	T4421	punct	),Roche
R293	T549	T550	npadvmod	cadmium,binding
R2930	T4423	T4386	prep	according,determined
R2931	T4424	T4423	prep	to,according
R2932	T4425	T4426	det	the,manufacturer
R2933	T4426	T4427	poss	manufacturer,instructions
R2934	T4427	T4424	pobj	instructions,to
R2935	T4428	T4426	case	's,manufacturer
R2936	T4429	T4386	punct	.,determined
R2939	T4680	T4679	prep	of,Generation
R294	T550	T548	amod	binding,antioxidant
R2940	T4681	T4682	det	an,line
R2941	T4682	T4680	pobj	line,of
R2942	T4683	T4682	amod	inducible,line
R2943	T4684	T4682	punct	", ",line
R2944	T4685	T4686	npadvmod	liver,specific
R2945	T4686	T4682	amod	specific,line
R2946	T4687	T4686	punct	-,specific
R2947	T4688	T4689	compound	Mtf1,knockout
R2948	T4689	T4682	compound	knockout,line
R2949	T4690	T4682	compound	mouse,line
R295	T551	T550	punct	-,binding
R2950	T4692	T4693	advcl	Using,obtained
R2951	T4694	T4695	det	a,strategy
R2952	T4695	T4692	dobj	strategy,Using
R2953	T4696	T4695	amod	homologous,strategy
R2954	T4697	T4695	compound	recombination,strategy
R2955	T4698	T4693	punct	", ",obtained
R2956	T4699	T4693	nsubjpass	mice,obtained
R2957	T4700	T4693	auxpass	were,obtained
R2958	T4701	T4693	prep	with,obtained
R2959	T4702	T4703	det	a,Mtf1loxP
R296	T552	T523	punct	.,induced
R2960	T4703	T4701	pobj	Mtf1loxP,with
R2961	T4704	T4703	amod	modified,Mtf1loxP
R2962	T4705	T4703	compound	Mtf1,Mtf1loxP
R2963	T4706	T4703	compound	allele,Mtf1loxP
R2964	T4707	T4708	advmod	where,flanked
R2965	T4708	T4693	advcl	flanked,obtained
R2966	T4709	T4710	nmod	exons,3
R2967	T4710	T4708	nsubjpass	3,flanked
R2968	T4711	T4710	cc	and,3
R2969	T4712	T4710	conj	4,3
R297	T554	T555	det	These,data
R2970	T4713	T4710	punct	", ",3
R2971	T4714	T4710	acl	encoding,3
R2972	T4715	T4714	dobj	four,encoding
R2973	T4716	T4715	prep	of,four
R2974	T4717	T4718	det	the,fingers
R2975	T4718	T4716	pobj	fingers,of
R2976	T4719	T4718	nummod	six,fingers
R2977	T4720	T4718	compound	zinc,fingers
R2978	T4721	T4718	prep	of,fingers
R2979	T4722	T4723	det	the,domain
R298	T555	T556	nsubj	data,provide
R2980	T4723	T4721	pobj	domain,of
R2981	T4724	T4725	npadvmod	DNA,binding
R2982	T4725	T4723	amod	binding,domain
R2983	T4726	T4725	punct	-,binding
R2984	T4727	T4708	punct	", ",flanked
R2985	T4728	T4708	auxpass	are,flanked
R2986	T4729	T4708	agent	by,flanked
R2987	T4730	T4731	compound	loxP,sites
R2988	T4731	T4729	pobj	sites,by
R2989	T4732	T4733	punct	(,1a
R299	T557	T558	amod	strong,evidence
R2990	T4733	T4708	parataxis	1a,flanked
R2991	T4734	T4733	compound	Figure,1a
R2992	T4735	T4733	punct	),1a
R2993	T4736	T4693	punct	.,obtained
R2994	T4738	T4739	nsubjpass	Mice,crossed
R2995	T4740	T4738	amod	homozygous,Mice
R2996	T4741	T4740	prep	for,homozygous
R2997	T4742	T4743	det	the,allele
R2998	T4743	T4741	pobj	allele,for
R2999	T4744	T4743	compound	Mtf1loxP,allele
R30	T268	T267	punct	-,MTF
R300	T558	T556	dobj	evidence,provide
R3000	T4745	T4739	auxpass	were,crossed
R3001	T4746	T4739	advmod	further,crossed
R3002	T4747	T4739	prep	with,crossed
R3003	T4748	T4747	pobj	animals,with
R3004	T4749	T4748	prep	of,animals
R3005	T4750	T4751	det	the,line
R3006	T4751	T4749	pobj	line,of
R3007	T4752	T4753	nmod	Cre,recombinase
R3008	T4753	T4751	nmod	recombinase,line
R3009	T4754	T4751	amod	transgenic,line
R301	T559	T558	prep	for,evidence
R3010	T4755	T4756	compound	Mx,cre
R3011	T4756	T4751	appos	cre,line
R3012	T4757	T4756	punct	-,cre
R3013	T4758	T4739	punct	.,crossed
R3014	T4760	T4761	compound	Cre,recombinase
R3015	T4761	T4762	nsubjpass	recombinase,expressed
R3016	T4763	T4762	auxpass	is,expressed
R3017	T4764	T4762	prep	in,expressed
R3018	T4765	T4766	det	this,line
R3019	T4766	T4764	pobj	line,in
R302	T560	T561	nummod	two,branches
R3020	T4767	T4762	prep	under,expressed
R3021	T4768	T4769	det	the,control
R3022	T4769	T4767	pobj	control,under
R3023	T4770	T4769	prep	of,control
R3024	T4771	T4772	det	the,promoter
R3025	T4772	T4770	pobj	promoter,of
R3026	T4773	T4772	compound	mouse,promoter
R3027	T4774	T4772	compound	Mx1,promoter
R3028	T4775	T4772	compound	gene,promoter
R3029	T4776	T4772	punct	", ",promoter
R303	T561	T559	pobj	branches,for
R3030	T4777	T4778	dep	which,is
R3031	T4778	T4772	relcl	is,promoter
R3032	T4779	T4778	acomp	inducible,is
R3033	T4780	T4779	prep	by,inducible
R3034	T4781	T4780	pobj	administration,by
R3035	T4782	T4781	prep	of,administration
R3036	T4783	T4784	compound	interferon,alpha
R3037	T4784	T4782	pobj	alpha,of
R3038	T4785	T4784	cc	or,alpha
R3039	T4786	T4784	conj	beta,alpha
R304	T562	T561	amod	major,branches
R3040	T4787	T4784	punct	", ",alpha
R3041	T4788	T4784	cc	or,alpha
R3042	T4789	T4790	amod	synthetic,pC
R3043	T4790	T4784	conj	pC,alpha
R3044	T4791	T4792	amod	double,stranded
R3045	T4792	T4790	amod	stranded,pC
R3046	T4793	T4792	punct	-,stranded
R3047	T4794	T4790	compound	RNA,pC
R3048	T4795	T4790	compound	pI,pC
R3049	T4796	T4790	punct	–,pC
R305	T563	T561	prep	of,branches
R3050	T4797	T4798	punct	(,32
R3051	T4798	T4778	parataxis	32,is
R3052	T4799	T4798	punct	),32
R3053	T4800	T4762	punct	.,expressed
R3054	T4802	T4803	npadvmod	Cre,mediated
R3055	T4803	T4805	amod	mediated,deletion
R3056	T4804	T4803	punct	-,mediated
R3057	T4805	T4806	nsubjpass	deletion,reported
R3058	T4807	T4806	auxpass	was,reported
R3059	T4808	T4809	aux	to,be
R306	T564	T565	amod	cellular,defense
R3060	T4809	T4806	xcomp	be,reported
R3061	T4810	T4809	acomp	complete,be
R3062	T4811	T4809	prep	in,be
R3063	T4812	T4813	det	the,liver
R3064	T4813	T4811	pobj	liver,in
R3065	T4814	T4809	punct	", ",be
R3066	T4815	T4816	mark	while,varying
R3067	T4816	T4809	advcl	varying,be
R3068	T4817	T4816	prep	in,varying
R3069	T4818	T4819	amod	other,tissues
R307	T565	T563	pobj	defense,of
R3070	T4819	T4817	pobj	tissues,in
R3071	T4820	T4816	punct	", ",varying
R3072	T4821	T4816	advcl	ranging,varying
R3073	T4822	T4821	prep	from,ranging
R3074	T4823	T4824	nummod	94,%
R3075	T4824	T4822	pobj	%,from
R3076	T4825	T4824	prep	in,%
R3077	T4826	T4825	pobj	spleen,in
R3078	T4827	T4822	prep	to,from
R3079	T4828	T4829	nummod	8,%
R308	T566	T565	amod	anti-cadmium,defense
R3080	T4829	T4827	pobj	%,to
R3081	T4830	T4829	prep	in,%
R3082	T4831	T4830	pobj	brain,in
R3083	T4832	T4833	punct	(,32
R3084	T4833	T4816	parataxis	32,varying
R3085	T4834	T4833	punct	),32
R3086	T4835	T4806	punct	.,reported
R3087	T4837	T4838	prep	After,produced
R3088	T4839	T4840	npadvmod	Cre,mediated
R3089	T4840	T4842	amod	mediated,deletion
R309	T567	T561	punct	", ",branches
R3090	T4841	T4840	punct	-,mediated
R3091	T4842	T4837	pobj	deletion,After
R3092	T4843	T4842	prep	of,deletion
R3093	T4844	T4845	nmod	exons,3
R3094	T4845	T4843	pobj	3,of
R3095	T4846	T4845	cc	and,3
R3096	T4847	T4845	conj	4,3
R3097	T4848	T4842	punct	", ",deletion
R3098	T4849	T4850	dep	which,results
R3099	T4850	T4842	relcl	results,deletion
R31	T269	T267	nummod	1,MTF
R310	T568	T561	appos	one,branches
R3100	T4851	T4850	prep	in,results
R3101	T4852	T4853	det	a,stop
R3102	T4853	T4851	pobj	stop,in
R3103	T4854	T4853	nmod	frameshift,stop
R3104	T4855	T4854	cc	and,frameshift
R3105	T4856	T4857	amod	premature,translation
R3106	T4857	T4854	conj	translation,frameshift
R3107	T4858	T4838	punct	", ",produced
R3108	T4859	T4860	det	no,protein
R3109	T4860	T4838	nsubjpass	protein,produced
R311	T569	T568	prep	via,one
R3110	T4861	T4860	amod	functional,protein
R3111	T4862	T4860	nmod	MTF,protein
R3112	T4863	T4862	punct	-,MTF
R3113	T4864	T4862	nummod	1,MTF
R3114	T4865	T4838	aux	can,produced
R3115	T4866	T4838	auxpass	be,produced
R3116	T4867	T4838	punct	.,produced
R3117	T4869	T4870	prep	For,received
R3118	T4870	T4891	ccomp	received,received
R3119	T4871	T4869	pobj	induction,For
R312	T570	T569	pobj	MTF,via
R3120	T4872	T4871	prep	of,induction
R3121	T4873	T4874	compound	Cre,recombinase
R3122	T4874	T4872	pobj	recombinase,of
R3123	T4875	T4870	punct	", ",received
R3124	T4876	T4877	nmod	Mtf1,mice
R3125	T4877	T4870	nsubj	mice,received
R3126	T4878	T4879	amod	conditional,knockout
R3127	T4879	T4877	compound	knockout,mice
R3128	T4880	T4877	acl	harboring,mice
R3129	T4881	T4882	det	the,transgene
R313	T571	T570	punct	-,MTF
R3130	T4882	T4880	dobj	transgene,harboring
R3131	T4883	T4884	compound	Mx,cre
R3132	T4884	T4882	compound	cre,transgene
R3133	T4885	T4884	punct	-,cre
R3134	T4886	T4882	punct	(,transgene
R3135	T4887	T4888	compound	Mtf1Mx,cre
R3136	T4888	T4882	appos	cre,transgene
R3137	T4889	T4888	punct	-,cre
R3138	T4890	T4870	punct	),received
R3139	T4892	T4893	nummod	four,injections
R314	T572	T570	nummod	1,MTF
R3140	T4893	T4870	dobj	injections,received
R3141	T4894	T4893	amod	intraperitoneal,injections
R3142	T4895	T4896	compound	pI,pC
R3143	T4896	T4893	compound	pC,injections
R3144	T4897	T4896	punct	–,pC
R3145	T4898	T4870	prep	at,received
R3146	T4899	T4900	nummod	3,day
R3147	T4900	T4901	compound	day,intervals
R3148	T4901	T4898	pobj	intervals,at
R3149	T4902	T4903	punct	(,induction
R315	T573	T570	cc	and,MTF
R3150	T4903	T4870	parataxis	induction,received
R3151	T4904	T4905	compound	pI,pC
R3152	T4905	T4903	compound	pC,induction
R3153	T4906	T4905	punct	–,pC
R3154	T4907	T4903	punct	),induction
R3155	T4908	T4891	punct	;,received
R3156	T4909	T4910	compound	control,littermates
R3157	T4910	T4891	nsubj	littermates,received
R3158	T4911	T4910	prep	without,littermates
R3159	T4912	T4911	pobj	transgene,without
R316	T574	T575	poss	its,genes
R3160	T4913	T4912	punct	(,transgene
R3161	T4914	T4912	appos	Mtf1loxP,transgene
R3162	T4915	T4891	punct	),received
R3163	T4916	T4917	amod	similar,injections
R3164	T4917	T4891	dobj	injections,received
R3165	T4918	T4891	punct	.,received
R3166	T4920	T4921	advcl	Using,obtained
R3167	T4922	T4923	compound	RT,PCRs
R3168	T4923	T4920	dobj	PCRs,Using
R3169	T4924	T4923	punct	–,PCRs
R317	T575	T570	conj	genes,MTF
R3170	T4925	T4926	punct	(,1b
R3171	T4926	T4920	parataxis	1b,Using
R3172	T4927	T4926	compound	Figure,1b
R3173	T4928	T4926	punct	),1b
R3174	T4929	T4921	punct	", ",obtained
R3175	T4930	T4931	det	a,product
R3176	T4931	T4921	nsubjpass	product,obtained
R3177	T4932	T4931	amod	shortened,product
R3178	T4933	T4921	auxpass	was,obtained
R3179	T4934	T4921	prep	with,obtained
R318	T576	T575	compound	target,genes
R3180	T4935	T4934	pobj	RNA,with
R3181	T4936	T4935	prep	from,RNA
R3182	T4937	T4938	compound	Mtf1Mx,cre
R3183	T4938	T4940	compound	cre,livers
R3184	T4939	T4938	punct	-,cre
R3185	T4940	T4936	pobj	livers,from
R3186	T4941	T4921	punct	", ",obtained
R3187	T4942	T4921	advcl	indicating,obtained
R3188	T4943	T4944	det	a,excision
R3189	T4944	T4942	dobj	excision,indicating
R319	T577	T575	punct	", ",genes
R3190	T4945	T4944	amod	successful,excision
R3191	T4946	T4944	prep	of,excision
R3192	T4947	T4948	nmod	exons,3
R3193	T4948	T4946	pobj	3,of
R3194	T4949	T4948	cc	and,3
R3195	T4950	T4948	conj	4,3
R3196	T4951	T4948	prep	of,3
R3197	T4952	T4951	pobj	Mtf1,of
R3198	T4953	T4942	prep	in,indicating
R3199	T4954	T4955	det	these,animals
R32	T270	T264	punct	),regulates
R320	T578	T579	advmod	notably,metallothioneins
R3200	T4955	T4953	pobj	animals,in
R3201	T4956	T4921	punct	.,obtained
R3202	T4958	T4959	prep	On,observed
R3203	T4960	T4961	amod	close,examination
R3204	T4961	T4958	pobj	examination,On
R3205	T4962	T4959	punct	", ",observed
R3206	T4963	T4964	det	a,band
R3207	T4964	T4959	nsubjpass	band,observed
R3208	T4965	T4966	advmod	very,faint
R3209	T4966	T4964	amod	faint,band
R321	T579	T575	appos	metallothioneins,genes
R3210	T4967	T4964	relcl	similar,band
R3211	T4968	T4967	prep	in,similar
R3212	T4969	T4968	pobj	size,in
R3213	T4970	T4967	prep	to,similar
R3214	T4971	T4972	amod	full,length
R3215	T4972	T4974	compound	length,signal
R3216	T4973	T4972	punct	-,length
R3217	T4974	T4970	pobj	signal,to
R3218	T4975	T4959	auxpass	was,observed
R3219	T4976	T4959	advmod	also,observed
R322	T580	T568	punct	", ",one
R3220	T4977	T4959	prep	in,observed
R3221	T4978	T4979	det	those,mice
R3222	T4979	T4977	pobj	mice,in
R3223	T4980	T4959	punct	", ",observed
R3224	T4981	T4982	advmod	probably,due
R3225	T4982	T4959	prep	due,observed
R3226	T4983	T4982	pcomp	to,due
R3227	T4984	T4985	det	a,amount
R3228	T4985	T4982	pobj	amount,due
R3229	T4986	T4985	amod	low,amount
R323	T581	T582	det	the,other
R3230	T4987	T4985	prep	of,amount
R3231	T4988	T4989	amod	residual,mRNA
R3232	T4989	T4987	pobj	mRNA,of
R3233	T4990	T4991	amod	full,length
R3234	T4991	T4989	compound	length,mRNA
R3235	T4992	T4991	punct	-,length
R3236	T4993	T4989	compound	Mtf1,mRNA
R3237	T4994	T4959	punct	.,observed
R3238	T4996	T4997	det	The,level
R3239	T4997	T4998	nsubjpass	level,examined
R324	T582	T568	appos	other,one
R3240	T4998	T5006	ccomp	examined,was
R3241	T4999	T4997	prep	of,level
R3242	T5000	T5001	amod	functional,protein
R3243	T5001	T4999	pobj	protein,of
R3244	T5002	T5001	nmod	MTF,protein
R3245	T5003	T5002	punct	-,MTF
R3246	T5004	T5002	nummod	1,MTF
R3247	T5005	T4998	auxpass	was,examined
R3248	T5007	T4998	prep	by,examined
R3249	T5008	T5007	pobj	EMSA,by
R325	T583	T582	prep	via,other
R3250	T5009	T5010	punct	(,1c
R3251	T5010	T4998	parataxis	1c,examined
R3252	T5011	T5010	compound	Figure,1c
R3253	T5012	T5010	punct	),1c
R3254	T5013	T5006	punct	: ,was
R3255	T5014	T5015	nmod	MTF,complex
R3256	T5015	T5006	nsubj	complex,was
R3257	T5016	T5014	punct	-,MTF
R3258	T5017	T5014	nummod	1,MTF
R3259	T5018	T5015	compound	protein,complex
R326	T584	T583	pobj	glutathione,via
R3260	T5019	T5015	punct	–,complex
R3261	T5020	T5015	compound	DNA,complex
R3262	T5021	T5006	acomp	detectable,was
R3263	T5022	T5021	prep	with,detectable
R3264	T5023	T5024	compound	liver,extract
R3265	T5024	T5022	pobj	extract,with
R3266	T5025	T5024	compound	protein,extract
R3267	T5026	T5024	prep	from,extract
R3268	T5027	T5028	det	an,mouse
R3269	T5028	T5026	pobj	mouse,from
R327	T585	T582	punct	", ",other
R3270	T5029	T5028	compound	Mtf1loxP,mouse
R3271	T5030	T5028	compound	control,mouse
R3272	T5031	T5006	punct	", ",was
R3273	T5032	T5006	cc	but,was
R3274	T5033	T5034	det	no,protein
R3275	T5034	T5039	nsubjpass	protein,observed
R3276	T5035	T5034	amod	functional,protein
R3277	T5036	T5037	npadvmod	MRE,binding
R3278	T5037	T5034	amod	binding,protein
R3279	T5038	T5037	punct	-,binding
R328	T586	T582	prep	with,other
R3280	T5039	T5006	conj	observed,was
R3281	T5040	T5039	auxpass	was,observed
R3282	T5041	T5039	prep	with,observed
R3283	T5042	T5043	det	an,sample
R3284	T5043	T5041	pobj	sample,with
R3285	T5044	T5045	compound	Mtf1Mx,cre
R3286	T5045	T5043	compound	cre,sample
R3287	T5046	T5045	punct	-,cre
R3288	T5047	T5006	punct	.,was
R3289	T5049	T5050	advmod	Thus,was
R329	T587	T588	det	an,overlap
R3290	T5051	T5050	punct	", ",was
R3291	T5052	T5050	nsubj	deletion,was
R3292	T5053	T5052	prep	of,deletion
R3293	T5054	T5055	nmod	exons,3
R3294	T5055	T5053	pobj	3,of
R3295	T5056	T5055	cc	and,3
R3296	T5057	T5055	conj	4,3
R3297	T5058	T5055	prep	of,3
R3298	T5059	T5058	pobj	Mtf1,of
R3299	T5060	T5052	prep	in,deletion
R33	T271	T264	dobj	expression,regulates
R330	T588	T586	pobj	overlap,with
R3300	T5061	T5062	det	the,liver
R3301	T5062	T5060	pobj	liver,in
R3302	T5063	T5062	prep	of,liver
R3303	T5064	T5065	compound	Mtf1Mx,cre
R3304	T5065	T5067	compound	cre,mice
R3305	T5066	T5065	punct	-,cre
R3306	T5067	T5063	pobj	mice,of
R3307	T5068	T5069	advmod	virtually,complete
R3308	T5069	T5050	acomp	complete,was
R3309	T5070	T5050	punct	.,was
R331	T589	T588	amod	apparent,overlap
R3310	T5072	T5073	det	All,mice
R3311	T5073	T5079	nsubj	mice,were
R3312	T5074	T5073	amod	examined,mice
R3313	T5075	T5076	npadvmod	liver,specific
R3314	T5076	T5073	amod	specific,mice
R3315	T5077	T5076	punct	-,specific
R3316	T5078	T5073	compound	knockout,mice
R3317	T5080	T5079	acomp	viable,were
R3318	T5081	T5079	prep	under,were
R3319	T5082	T5083	compound	laboratory,conditions
R332	T590	T588	prep	in,overlap
R3320	T5083	T5081	pobj	conditions,under
R3321	T5084	T5079	cc	and,were
R3322	T5085	T5079	conj	appeared,were
R3323	T5086	T5085	oprd	normal,appeared
R3324	T5087	T5079	punct	.,were
R3325	T5337	T5338	nmod	MTF,search
R3326	T5339	T5337	punct	-,MTF
R3327	T5340	T5337	nummod	1,MTF
R3328	T5341	T5338	compound	target,search
R3329	T5342	T5338	compound	gene,search
R333	T591	T592	compound	selenoprotein,W
R3330	T5344	T5345	prep	For,compared
R3331	T5346	T5347	det	the,identification
R3332	T5347	T5344	pobj	identification,For
R3333	T5348	T5347	prep	of,identification
R3334	T5349	T5350	nmod	MTF,genes
R3335	T5350	T5348	pobj	genes,of
R3336	T5351	T5349	punct	-,MTF
R3337	T5352	T5349	nummod	1,MTF
R3338	T5353	T5350	compound	target,genes
R3339	T5354	T5345	punct	", ",compared
R334	T592	T590	pobj	W,in
R3340	T5355	T5345	nsubj	we,compared
R3341	T5356	T5357	det	the,profiles
R3342	T5357	T5345	dobj	profiles,compared
R3343	T5358	T5357	compound	liver,profiles
R3344	T5359	T5357	compound	transcript,profiles
R3345	T5360	T5357	prep	of,profiles
R3346	T5361	T5360	pobj	mice,of
R3347	T5362	T5361	prep	with,mice
R3348	T5363	T5362	cc	and,with
R3349	T5364	T5362	conj	without,with
R335	T593	T556	punct	.,provide
R3350	T5365	T5366	amod	functional,gene
R3351	T5366	T5364	pobj	gene,without
R3352	T5367	T5366	compound	Mtf1,gene
R3353	T5368	T5369	dep	that,treated
R3354	T5369	T5366	relcl	treated,gene
R3355	T5370	T5369	aux	had,treated
R3356	T5371	T5369	auxpass	been,treated
R3357	T5372	T5369	amod	mock,treated
R3358	T5373	T5369	punct	-,treated
R3359	T5374	T5369	cc	or,treated
R336	T239	T240	nummod	Two,branches
R3360	T5375	T5369	conj	exposed,treated
R3361	T5376	T5375	prep	to,exposed
R3362	T5377	T5376	pobj	cadmium,to
R3363	T5378	T5379	punct	(,3
R3364	T5379	T5375	parataxis	3,exposed
R3365	T5380	T5379	nsubj	n,3
R3366	T5381	T5379	punct	=,3
R3367	T5382	T5379	prep	per,3
R3368	T5383	T5382	pobj	genotype,per
R3369	T5384	T5379	cc	and,3
R337	T980	T981	det	All,organisms
R3370	T5385	T5386	amod	respective,treatment
R3371	T5386	T5379	conj	treatment,3
R3372	T5387	T5379	punct	),3
R3373	T5388	T5345	punct	.,compared
R3374	T5390	T5391	prep	In,analyzed
R3375	T5392	T5393	det	a,screen
R3376	T5393	T5390	pobj	screen,In
R3377	T5394	T5393	amod	first,screen
R3378	T5395	T5391	punct	", ",analyzed
R3379	T5396	T5397	det	the,transcripts
R338	T981	T982	nsubj	organisms,evolved
R3380	T5397	T5391	nsubjpass	transcripts,analyzed
R3381	T5398	T5391	auxpass	were,analyzed
R3382	T5399	T5391	prep	with,analyzed
R3383	T5400	T5401	det	a,method
R3384	T5401	T5399	pobj	method,with
R3385	T5402	T5401	amod	differential,method
R3386	T5403	T5404	npadvmod	display,based
R3387	T5404	T5401	amod	based,method
R3388	T5405	T5404	punct	-,based
R3389	T5406	T5391	punct	", ",analyzed
R339	T983	T982	aux	have,evolved
R3390	T5407	T5391	dep	called,analyzed
R3391	T5408	T5407	oprd	amplification,called
R3392	T5409	T5408	prep	of,amplification
R3393	T5410	T5411	amod	double,stranded
R3394	T5411	T5413	amod	stranded,fragments
R3395	T5412	T5411	punct	-,stranded
R3396	T5413	T5409	pobj	fragments,of
R3397	T5414	T5413	compound	cDNA,fragments
R3398	T5415	T5413	compound	end,fragments
R3399	T5416	T5413	compound	restriction,fragments
R34	T272	T271	prep	of,expression
R340	T984	T982	dobj	mechanisms,evolved
R3400	T5417	T5408	punct	(,amplification
R3401	T5418	T5408	appos	ADDER,amplification
R3402	T5419	T5407	punct	),called
R3403	T5420	T5421	punct	(,33
R3404	T5421	T5407	parataxis	33,called
R3405	T5422	T5421	punct	),33
R3406	T5423	T5391	punct	.,analyzed
R3407	T5425	T5426	advmod	Thereby,obtained
R3408	T5427	T5428	det	an,number
R3409	T5428	T5426	nsubjpass	number,obtained
R341	T985	T986	aux	to,cope
R3410	T5429	T5428	amod	overwhelming,number
R3411	T5430	T5428	prep	of,number
R3412	T5431	T5430	pobj	signals,of
R3413	T5432	T5426	auxpass	was,obtained
R3414	T5433	T5426	prep	for,obtained
R3415	T5434	T5435	det	the,metallothioneins
R3416	T5435	T5433	pobj	metallothioneins,for
R3417	T5436	T5435	nummod	two,metallothioneins
R3418	T5437	T5438	npadvmod	stress,inducible
R3419	T5438	T5435	amod	inducible,metallothioneins
R342	T986	T984	advcl	cope,mechanisms
R3420	T5439	T5438	punct	-,inducible
R3421	T5440	T5435	punct	(,metallothioneins
R3422	T5441	T5435	appos	Mt1,metallothioneins
R3423	T5442	T5441	cc	and,Mt1
R3424	T5443	T5441	conj	Mt2,Mt1
R3425	T5444	T5426	punct	),obtained
R3426	T5445	T5426	punct	", ",obtained
R3427	T5446	T5426	prep	due,obtained
R3428	T5447	T5446	pcomp	to,due
R3429	T5448	T5449	det	the,abundance
R343	T987	T986	prep	with,cope
R3430	T5449	T5446	pobj	abundance,due
R3431	T5450	T5449	prep	of,abundance
R3432	T5451	T5452	poss	their,transcripts
R3433	T5452	T5450	pobj	transcripts,of
R3434	T5453	T5454	preconj	both,in
R3435	T5454	T5449	prep	in,abundance
R3436	T5455	T5456	amod	mock,treated
R3437	T5456	T5454	pobj	treated,in
R3438	T5457	T5456	punct	-,treated
R3439	T5458	T5454	cc	and,in
R344	T988	T989	det	a,variety
R3440	T5459	T5460	advmod	especially,in
R3441	T5460	T5454	conj	in,in
R3442	T5461	T5462	npadvmod	cadmium,treated
R3443	T5462	T5460	pobj	treated,in
R3444	T5463	T5462	punct	-,treated
R3445	T5464	T5460	pobj	livers,in
R3446	T5465	T5466	dep	that,harbored
R3447	T5466	T5460	pcomp	harbored,in
R3448	T5467	T5468	det	a,gene
R3449	T5468	T5466	dobj	gene,harbored
R345	T989	T987	pobj	variety,with
R3450	T5469	T5468	amod	functional,gene
R3451	T5470	T5468	nmod	MTF,gene
R3452	T5471	T5470	punct	-,MTF
R3453	T5472	T5470	nummod	1,MTF
R3454	T5473	T5474	punct	(,shown
R3455	T5474	T5426	parataxis	shown,obtained
R3456	T5475	T5474	nsubj	data,shown
R3457	T5476	T5474	neg	not,shown
R3458	T5477	T5474	punct	),shown
R3459	T5478	T5426	punct	.,obtained
R346	T990	T989	prep	of,variety
R3460	T5480	T5481	det	This,result
R3461	T5481	T5482	nsubj	result,confirmed
R3462	T5483	T5484	det	the,importance
R3463	T5484	T5482	dobj	importance,confirmed
R3464	T5485	T5484	prep	of,importance
R3465	T5486	T5485	pobj	MTF,of
R3466	T5487	T5486	punct	-,MTF
R3467	T5488	T5486	nummod	1,MTF
R3468	T5489	T5484	prep	for,importance
R3469	T5490	T5491	preconj	both,basal
R347	T991	T992	compound	stress,situations
R3470	T5491	T5492	amod	basal,expression
R3471	T5492	T5489	pobj	expression,for
R3472	T5493	T5491	cc	and,basal
R3473	T5494	T5495	npadvmod	metal,induced
R3474	T5495	T5491	conj	induced,basal
R3475	T5496	T5495	punct	-,induced
R3476	T5497	T5492	prep	of,expression
R3477	T5498	T5499	compound	metallothionein,genes
R3478	T5499	T5497	pobj	genes,of
R3479	T5500	T5482	punct	.,confirmed
R348	T992	T990	pobj	situations,of
R3480	T5502	T5503	prep	In,compared
R3481	T5504	T5505	det	a,approach
R3482	T5505	T5502	pobj	approach,In
R3483	T5506	T5505	amod	second,approach
R3484	T5507	T5503	punct	", ",compared
R3485	T5508	T5509	det	the,profile
R3486	T5509	T5503	nsubjpass	profile,compared
R3487	T5510	T5511	compound	gene,expression
R3488	T5511	T5509	compound	expression,profile
R3489	T5512	T5509	prep	in,profile
R349	T993	T982	punct	.,evolved
R3490	T5513	T5512	pobj	livers,in
R3491	T5514	T5513	prep	of,livers
R3492	T5515	T5516	det	the,mice
R3493	T5516	T5514	pobj	mice,of
R3494	T5517	T5518	advmod	above,mentioned
R3495	T5518	T5516	amod	mentioned,mice
R3496	T5519	T5503	auxpass	was,compared
R3497	T5520	T5503	agent	by,compared
R3498	T5521	T5522	nmod	Affymetrix,GeneChip
R3499	T5522	T5523	nmod	GeneChip,Arrays
R35	T273	T274	poss	its,genes
R350	T995	T996	nummod	One,type
R3500	T5523	T5520	pobj	Arrays,by
R3501	T5524	T5522	punct	®,GeneChip
R3502	T5525	T5526	nmod	Mouse,Genome
R3503	T5526	T5523	nmod	Genome,Arrays
R3504	T5527	T5526	nummod	430,Genome
R3505	T5528	T5526	nummod	2.0,Genome
R3506	T5529	T5530	punct	(,Table
R3507	T5530	T5503	parataxis	Table,compared
R3508	T5531	T5530	nummod	1,Table
R3509	T5532	T5530	punct	),Table
R351	T996	T997	nsubjpass	type,triggered
R3510	T5533	T5503	punct	.,compared
R3511	T5535	T5536	advmod	When,analyzing
R3512	T5536	T5537	advcl	analyzing,detected
R3513	T5538	T5539	det	the,data
R3514	T5539	T5536	dobj	data,analyzing
R3515	T5540	T5539	compound	probe,data
R3516	T5541	T5539	compound	array,data
R3517	T5542	T5539	prep	of,data
R3518	T5543	T5542	pobj	livers,of
R3519	T5544	T5543	prep	from,livers
R352	T998	T996	prep	of,type
R3520	T5545	T5546	amod	mock,treated
R3521	T5546	T5548	amod	treated,mice
R3522	T5547	T5546	punct	-,treated
R3523	T5548	T5544	pobj	mice,from
R3524	T5549	T5550	nmod	Mtf1Mx,cre
R3525	T5550	T5548	nmod	cre,mice
R3526	T5551	T5550	punct	-,cre
R3527	T5552	T5550	cc	and,cre
R3528	T5553	T5550	conj	Mtf1loxP,cre
R3529	T5554	T5537	punct	", ",detected
R353	T999	T1000	compound	stress,response
R3530	T5555	T5556	det	an,downregulation
R3531	T5556	T5537	nsubjpass	downregulation,detected
R3532	T5557	T5558	advmod	at,least
R3533	T5558	T5559	advmod	least,2-fold
R3534	T5559	T5556	nummod	2-fold,downregulation
R3535	T5560	T5556	punct	", ",downregulation
R3536	T5561	T5556	amod	reliable,downregulation
R3537	T5562	T5556	prep	of,downregulation
R3538	T5563	T5562	pobj	expression,of
R3539	T5564	T5537	auxpass	was,detected
R354	T1000	T998	pobj	response,of
R3540	T5565	T5537	prep	in,detected
R3541	T5566	T5567	compound	Mtf1Mx,cre
R3542	T5567	T5569	compound	cre,livers
R3543	T5568	T5567	punct	-,cre
R3544	T5569	T5565	pobj	livers,in
R3545	T5570	T5537	prep	for,detected
R3546	T5571	T5572	nummod	13,sets
R3547	T5572	T5570	pobj	sets,for
R3548	T5573	T5574	nmod	Affymetrix,GeneChip
R3549	T5574	T5572	nmod	GeneChip,sets
R355	T1001	T997	auxpass	is,triggered
R3550	T5575	T5574	punct	®,GeneChip
R3551	T5576	T5572	compound	probe,sets
R3552	T5577	T5572	acl	corresponding,sets
R3553	T5578	T5577	prep	to,corresponding
R3554	T5579	T5580	nummod	11,genes
R3555	T5580	T5578	pobj	genes,to
R3556	T5581	T5580	amod	characterized,genes
R3557	T5582	T5583	punct	(,a
R3558	T5583	T5537	parataxis	a,detected
R3559	T5584	T5583	nmod	Table,a
R356	T1002	T997	agent	by,triggered
R3560	T5585	T5583	nummod	1,a
R3561	T5586	T5583	punct	", ",a
R3562	T5587	T5583	punct	),a
R3563	T5588	T5537	punct	.,detected
R3564	T5590	T5591	nsubj	Seven,contain
R3565	T5592	T5590	prep	of,Seven
R3566	T5593	T5594	det	these,genes
R3567	T5594	T5592	pobj	genes,of
R3568	T5595	T5596	nummod	one,TGCRCNC
R3569	T5596	T5591	dobj	TGCRCNC,contain
R357	T1003	T1004	amod	heavy,metals
R3570	T5597	T5595	cc	or,one
R3571	T5598	T5595	conj	more,one
R3572	T5599	T5600	compound	MRE,core
R3573	T5600	T5596	compound	core,TGCRCNC
R3574	T5601	T5596	compound	consensus,TGCRCNC
R3575	T5602	T5596	compound	sequence,TGCRCNC
R3576	T5603	T5596	prep	within,TGCRCNC
R3577	T5604	T5605	det	a,segment
R3578	T5605	T5603	pobj	segment,within
R3579	T5606	T5605	prep	of,segment
R358	T1004	T1002	pobj	metals,by
R3580	T5607	T5608	nummod	1000,bp
R3581	T5608	T5606	pobj	bp,of
R3582	T5609	T5605	advmod	upstream,segment
R3583	T5610	T5609	prep	of,upstream
R3584	T5611	T5612	det	the,start
R3585	T5612	T5610	pobj	start,of
R3586	T5613	T5612	compound	transcription,start
R3587	T5614	T5591	punct	.,contain
R3588	T5616	T5617	prep	For,detected
R3589	T5617	T5636	ccomp	detected,contain
R359	T1005	T1004	punct	", ",metals
R3590	T5618	T5619	nummod	26,sets
R3591	T5619	T5616	pobj	sets,For
R3592	T5620	T5619	compound	probe,sets
R3593	T5621	T5619	acl	corresponding,sets
R3594	T5622	T5621	prep	to,corresponding
R3595	T5623	T5624	nummod	24,genes
R3596	T5624	T5622	pobj	genes,to
R3597	T5625	T5624	amod	different,genes
R3598	T5626	T5624	amod	characterized,genes
R3599	T5627	T5617	punct	", ",detected
R36	T274	T272	pobj	genes,of
R360	T1006	T1007	amod	such,as
R3600	T5628	T5629	det	a,upregulation
R3601	T5629	T5617	nsubjpass	upregulation,detected
R3602	T5630	T5629	amod	2-fold,upregulation
R3603	T5631	T5630	cc	or,2-fold
R3604	T5632	T5630	conj	higher,2-fold
R3605	T5633	T5629	punct	", ",upregulation
R3606	T5634	T5629	amod	reliable,upregulation
R3607	T5635	T5617	auxpass	was,detected
R3608	T5637	T5617	prep	in,detected
R3609	T5638	T5639	compound	Mtf1Mx,cre
R361	T1007	T1004	prep	as,metals
R3610	T5639	T5641	compound	cre,livers
R3611	T5640	T5639	punct	-,cre
R3612	T5641	T5637	pobj	livers,in
R3613	T5642	T5643	punct	(,b
R3614	T5643	T5641	parataxis	b,livers
R3615	T5644	T5643	nmod	Table,b
R3616	T5645	T5643	nummod	1,b
R3617	T5646	T5643	punct	", ",b
R3618	T5647	T5643	punct	),b
R3619	T5648	T5636	punct	;,contain
R362	T1008	T1007	pobj	zinc,as
R3620	T5649	T5636	nsubj	17,contain
R3621	T5650	T5649	prep	of,17
R3622	T5651	T5652	det	these,genes
R3623	T5652	T5650	pobj	genes,of
R3624	T5653	T5652	nummod	24,genes
R3625	T5654	T5655	compound	MRE,core
R3626	T5655	T5656	compound	core,sequences
R3627	T5656	T5636	dobj	sequences,contain
R3628	T5657	T5656	compound	consensus,sequences
R3629	T5658	T5636	prep	in,contain
R363	T1009	T1008	punct	", ",zinc
R3630	T5659	T5660	det	the,region
R3631	T5660	T5658	pobj	region,in
R3632	T5661	T5660	amod	upstream,region
R3633	T5662	T5636	punct	.,contain
R3634	T5664	T5665	det	The,set
R3635	T5665	T5667	nsubj	set,revealed
R3636	T5666	T5665	compound	data,set
R3637	T5667	T5680	ccomp	revealed,contain
R3638	T5668	T5665	prep	for,set
R3639	T5669	T5668	pobj	livers,for
R364	T1010	T1008	conj	copper,zinc
R3640	T5670	T5669	prep	of,livers
R3641	T5671	T5672	npadvmod	cadmium,treated
R3642	T5672	T5674	amod	treated,mice
R3643	T5673	T5672	punct	-,treated
R3644	T5674	T5670	pobj	mice,of
R3645	T5675	T5676	nmod	Mtf1Mx,cre
R3646	T5676	T5674	nmod	cre,mice
R3647	T5677	T5676	punct	-,cre
R3648	T5678	T5676	cc	and,cre
R3649	T5679	T5676	conj	Mtf1loxP,cre
R365	T1011	T1010	cc	and,copper
R3650	T5681	T5682	det	an,downregulation
R3651	T5682	T5667	dobj	downregulation,revealed
R3652	T5683	T5684	advmod	at,least
R3653	T5684	T5685	advmod	least,2-fold
R3654	T5685	T5682	amod	2-fold,downregulation
R3655	T5686	T5682	punct	", ",downregulation
R3656	T5687	T5682	amod	reliable,downregulation
R3657	T5688	T5667	prep	in,revealed
R3658	T5689	T5690	compound	Mtf1Mx,cre
R3659	T5690	T5692	compound	cre,livers
R366	T1012	T1010	conj	cadmium,copper
R3660	T5691	T5690	punct	-,cre
R3661	T5692	T5688	pobj	livers,in
R3662	T5693	T5667	prep	for,revealed
R3663	T5694	T5695	nummod	21,sets
R3664	T5695	T5693	pobj	sets,for
R3665	T5696	T5695	compound	probe,sets
R3666	T5697	T5695	acl	corresponding,sets
R3667	T5698	T5697	prep	to,corresponding
R3668	T5699	T5700	nummod	16,genes
R3669	T5700	T5698	pobj	genes,to
R367	T1013	T1014	punct	(,used
R3670	T5701	T5700	amod	different,genes
R3671	T5702	T5700	amod	characterized,genes
R3672	T5703	T5704	punct	(,c
R3673	T5704	T5667	parataxis	c,revealed
R3674	T5705	T5704	nmod	Table,c
R3675	T5706	T5704	nummod	1,c
R3676	T5707	T5704	punct	", ",c
R3677	T5708	T5704	punct	),c
R3678	T5709	T5680	punct	;,contain
R3679	T5710	T5680	nsubj	10,contain
R368	T1014	T1002	parataxis	used,by
R3680	T5711	T5710	prep	of,10
R3681	T5712	T5711	pobj	these,of
R3682	T5713	T5714	compound	MRE,sequences
R3683	T5714	T5680	dobj	sequences,contain
R3684	T5715	T5714	compound	core,sequences
R3685	T5716	T5714	compound	consensus,sequences
R3686	T5717	T5680	prep	in,contain
R3687	T5718	T5719	poss	their,region
R3688	T5719	T5717	pobj	region,in
R3689	T5720	T5719	amod	upstream,region
R369	T1015	T1014	prep	for,used
R3690	T5721	T5680	punct	.,contain
R3691	T5723	T5724	prep	For,detected
R3692	T5724	T5743	ccomp	detected,contain
R3693	T5725	T5726	nummod	9,sets
R3694	T5726	T5723	pobj	sets,For
R3695	T5727	T5726	compound	probe,sets
R3696	T5728	T5726	acl	corresponding,sets
R3697	T5729	T5728	prep	to,corresponding
R3698	T5730	T5731	nummod	9,genes
R3699	T5731	T5729	pobj	genes,to
R37	T275	T274	compound	target,genes
R370	T1016	T1015	pobj	convenience,for
R3700	T5732	T5731	amod	different,genes
R3701	T5733	T5731	amod	characterized,genes
R3702	T5734	T5724	punct	", ",detected
R3703	T5735	T5736	det	an,upregulation
R3704	T5736	T5724	nsubjpass	upregulation,detected
R3705	T5737	T5738	advmod	at,least
R3706	T5738	T5739	advmod	least,2-fold
R3707	T5739	T5736	nummod	2-fold,upregulation
R3708	T5740	T5736	punct	", ",upregulation
R3709	T5741	T5736	amod	reliable,upregulation
R371	T1017	T1014	punct	", ",used
R3710	T5742	T5724	auxpass	was,detected
R3711	T5744	T5745	punct	(,d
R3712	T5745	T5724	parataxis	d,detected
R3713	T5746	T5745	nmod	Table,d
R3714	T5747	T5745	nummod	1,d
R3715	T5748	T5745	punct	", ",d
R3716	T5749	T5745	punct	),d
R3717	T5750	T5743	punct	;,contain
R3718	T5751	T5743	nsubj	five,contain
R3719	T5752	T5751	prep	of,five
R372	T1018	T1019	det	the,zinc
R3720	T5753	T5752	pobj	them,of
R3721	T5754	T5755	compound	MRE,motifs
R3722	T5755	T5743	dobj	motifs,contain
R3723	T5756	T5743	punct	.,contain
R3724	T5758	T5759	prep	In,found
R3725	T5760	T5758	pobj	addition,In
R3726	T5761	T5760	prep	to,addition
R3727	T5762	T5763	amod	characterized,genes
R3728	T5763	T5761	pobj	genes,to
R3729	T5764	T5759	punct	", ",found
R373	T1019	T1014	nsubjpass	zinc,used
R3730	T5765	T5759	nsubjpass	ESTs,found
R3731	T5766	T5765	cc	and,ESTs
R3732	T5767	T5768	compound	RIKEN,cDNA
R3733	T5768	T5769	compound	cDNA,sequences
R3734	T5769	T5765	conj	sequences,ESTs
R3735	T5770	T5759	auxpass	were,found
R3736	T5771	T5759	advmod	also,found
R3737	T5772	T5759	prep	in,found
R3738	T5773	T5774	det	the,comparison
R3739	T5774	T5772	pobj	comparison,in
R374	T1020	T1019	compound	terms,zinc
R3740	T5775	T5774	prep	of,comparison
R3741	T5776	T5777	nmod	Mtf1Mx,cre
R3742	T5777	T5779	nmod	cre,livers
R3743	T5778	T5777	punct	-,cre
R3744	T5779	T5775	pobj	livers,of
R3745	T5780	T5777	cc	and,cre
R3746	T5781	T5777	conj	Mtf1loxP,cre
R3747	T5782	T5783	aux	to,expressed
R3748	T5783	T5759	xcomp	expressed,found
R3749	T5784	T5783	auxpass	be,expressed
R375	T1021	T1019	punct	", ",zinc
R3750	T5785	T5783	advmod	differentially,expressed
R3751	T5786	T5787	punct	(,Table
R3752	T5787	T5759	parataxis	Table,found
R3753	T5788	T5787	amod	Supplementary,Table
R3754	T5789	T5787	nummod	1,Table
R3755	T5790	T5787	punct	),Table
R3756	T5791	T5759	punct	.,found
R3757	T5793	T5794	nsubjpass	Downregulation,detected
R3758	T5795	T5793	prep	of,Downregulation
R3759	T5796	T5795	pobj	Mt1,of
R376	T1022	T1019	conj	copper,zinc
R3760	T5797	T5796	cc	and,Mt1
R3761	T5798	T5796	conj	Mt2,Mt1
R3762	T5799	T5794	auxpass	was,detected
R3763	T5800	T5794	prep	in,detected
R3764	T5801	T5802	compound	Mtf1Mx,cre
R3765	T5802	T5804	compound	cre,livers
R3766	T5803	T5802	punct	-,cre
R3767	T5804	T5800	pobj	livers,in
R3768	T5805	T5794	prep	for,detected
R3769	T5806	T5807	det	both,conditions
R377	T1023	T1022	cc	and,copper
R3770	T5807	T5805	pobj	conditions,for
R3771	T5808	T5794	punct	(,detected
R3772	T5809	T5810	mark	though,was
R3773	T5810	T5794	advcl	was,detected
R3774	T5811	T5812	det	the,level
R3775	T5812	T5810	nsubj	level,was
R3776	T5813	T5812	prep	of,level
R3777	T5814	T5813	pobj	significance,of
R3778	T5815	T5812	prep	for,level
R3779	T5816	T5817	det	the,downregulation
R378	T1024	T1022	conj	cadmium,copper
R3780	T5817	T5815	pobj	downregulation,for
R3781	T5818	T5817	prep	of,downregulation
R3782	T5819	T5818	pobj	Mt1,of
R3783	T5820	T5812	prep	in,level
R3784	T5821	T5822	amod	mock,treated
R3785	T5822	T5824	amod	treated,animals
R3786	T5823	T5822	punct	-,treated
R3787	T5824	T5820	pobj	animals,in
R3788	T5825	T5826	quantmod	above,0.05
R3789	T5826	T5810	attr	0.05,was
R379	T1025	T1014	auxpass	are,used
R3790	T5827	T5810	punct	;,was
R3791	T5828	T5829	nsubj	data,shown
R3792	T5829	T5810	parataxis	shown,was
R3793	T5830	T5829	neg	not,shown
R3794	T5831	T5794	punct	),detected
R3795	T5832	T5794	punct	.,detected
R3796	T5834	T5835	prep	For,exerts
R3797	T5836	T5837	det	all,genes
R3798	T5837	T5834	pobj	genes,For
R3799	T5838	T5837	nmod	MTF,genes
R38	T276	T264	prep	in,regulates
R380	T1026	T1014	advmod	also,used
R3800	T5839	T5838	punct	-,MTF
R3801	T5840	T5838	nummod	1,MTF
R3802	T5841	T5837	compound	target,genes
R3803	T5842	T5837	acl	characterized,genes
R3804	T5843	T5844	advmod	so,far
R3805	T5844	T5842	advmod	far,characterized
R3806	T5845	T5837	punct	", ",genes
R3807	T5846	T5847	amod	such,as
R3808	T5847	T5837	prep	as,genes
R3809	T5848	T5847	pobj	Mt1,as
R381	T1027	T1014	advmod	here,used
R3810	T5849	T5848	punct	", ",Mt1
R3811	T5850	T5848	conj	Mt2,Mt1
R3812	T5851	T5850	cc	and,Mt2
R3813	T5852	T5850	conj	Znt1,Mt2
R3814	T5853	T5835	punct	", ",exerts
R3815	T5854	T5835	nsubj	MTF,exerts
R3816	T5855	T5854	punct	-,MTF
R3817	T5856	T5854	nummod	1,MTF
R3818	T5857	T5858	poss	its,activity
R3819	T5858	T5835	dobj	activity,exerts
R382	T1028	T1029	aux	to,denote
R3820	T5859	T5860	amod	transcriptional,activation
R3821	T5860	T5858	compound	activation,activity
R3822	T5861	T5835	prep	via,exerts
R3823	T5862	T5863	amod	standard,sequences
R3824	T5863	T5861	pobj	sequences,via
R3825	T5864	T5863	compound	MRE,sequences
R3826	T5865	T5863	acl	located,sequences
R3827	T5866	T5865	advmod	proximal,located
R3828	T5867	T5866	prep	to,proximal
R3829	T5868	T5869	det	the,start
R383	T1029	T1014	advcl	denote,used
R3830	T5869	T5867	pobj	start,to
R3831	T5870	T5869	compound	transcription,start
R3832	T5871	T5872	punct	(,19
R3833	T5872	T5835	parataxis	19,exerts
R3834	T5873	T5872	nummod	4,19
R3835	T5874	T5872	punct	",",19
R3836	T5875	T5872	nummod	5,19
R3837	T5876	T5872	punct	",",19
R3838	T5877	T5872	nummod	8,19
R3839	T5878	T5872	punct	",",19
R384	T1030	T1029	dobj	Zn2,denote
R3840	T5879	T5872	nummod	18,19
R3841	T5880	T5872	punct	",",19
R3842	T5881	T5872	punct	),19
R3843	T5882	T5835	punct	.,exerts
R3844	T5884	T5885	advmod	Even,search
R3845	T5885	T5888	nsubj	search,yielded
R3846	T5886	T5885	det	a,search
R3847	T5887	T5885	amod	specific,search
R3848	T5889	T5885	prep	for,search
R3849	T5890	T5891	nmod	MTF,sites
R385	T1031	T1030	punct	+,Zn2
R3850	T5891	T5889	pobj	sites,for
R3851	T5892	T5890	punct	-,MTF
R3852	T5893	T5890	nummod	1,MTF
R3853	T5894	T5891	compound	binding,sites
R3854	T5895	T5885	prep	by,search
R3855	T5896	T5895	pobj	selection,by
R3856	T5897	T5896	prep	from,selection
R3857	T5898	T5899	det	a,pool
R3858	T5899	T5897	pobj	pool,from
R3859	T5900	T5899	prep	of,pool
R386	T1032	T1030	punct	", ",Zn2
R3860	T5901	T5902	amod	double,stranded
R3861	T5902	T5904	amod	stranded,oligonucleotides
R3862	T5903	T5902	punct	-,stranded
R3863	T5904	T5900	pobj	oligonucleotides,of
R3864	T5905	T5904	prep	with,oligonucleotides
R3865	T5906	T5907	amod	random,sequences
R3866	T5907	T5905	pobj	sequences,with
R3867	T5908	T5909	det	no,motif
R3868	T5909	T5888	dobj	motif,yielded
R3869	T5910	T5909	amod	new,motif
R387	T1033	T1030	conj	Cu2,Zn2
R3870	T5911	T5909	compound	binding,motif
R3871	T5912	T5909	prep	for,motif
R3872	T5913	T5912	pobj	MTF,for
R3873	T5914	T5913	punct	-,MTF
R3874	T5915	T5913	nummod	1,MTF
R3875	T5916	T5909	prep	in,motif
R3876	T5917	T5916	pobj	addition,in
R3877	T5918	T5917	prep	to,addition
R3878	T5919	T5920	det	the,MREs
R3879	T5920	T5918	pobj	MREs,to
R388	T1034	T1033	punct	+,Cu2
R3880	T5921	T5920	amod	known,MREs
R3881	T5922	T5923	punct	(,34
R3882	T5923	T5888	parataxis	34,yielded
R3883	T5924	T5923	punct	),34
R3884	T5925	T5888	punct	.,yielded
R3885	T5927	T5928	advmod	Thus,is
R3886	T5929	T5928	punct	", ",is
R3887	T5930	T5931	det	an,sequence
R3888	T5931	T5928	nsubj	sequence,is
R3889	T5932	T5931	compound	MRE,sequence
R389	T1035	T1033	cc	and,Cu2
R3890	T5933	T5928	prep	to,is
R3891	T5934	T5933	pobj	date,to
R3892	T5935	T5936	det	the,indication
R3893	T5936	T5928	attr	indication,is
R3894	T5937	T5936	amod	only,indication
R3895	T5938	T5936	prep	for,indication
R3896	T5939	T5940	det	a,gene
R3897	T5940	T5938	pobj	gene,for
R3898	T5941	T5940	amod	direct,gene
R3899	T5942	T5940	nmod	MTF,gene
R39	T277	T276	pobj	response,in
R390	T1036	T1033	conj	Cd2,Cu2
R3900	T5943	T5942	punct	-,MTF
R3901	T5944	T5942	nummod	1,MTF
R3902	T5945	T5940	compound	target,gene
R3903	T5946	T5928	punct	", ",is
R3904	T5947	T5928	cc	and,is
R3905	T5948	T5949	nummod	four,candidates
R3906	T5949	T5955	nsubjpass	candidates,analyzed
R3907	T5950	T5951	npadvmod	MRE,containing
R3908	T5951	T5949	amod	containing,candidates
R3909	T5952	T5951	punct	-,containing
R391	T1037	T1036	punct	+,Cd2
R3910	T5953	T5949	compound	target,candidates
R3911	T5954	T5949	compound	gene,candidates
R3912	T5955	T5928	conj	analyzed,is
R3913	T5956	T5955	auxpass	were,analyzed
R3914	T5957	T5955	advmod	further,analyzed
R3915	T5958	T5955	punct	.,analyzed
R3916	T6000	T6001	amod	Basal,expression
R3917	T6001	T6002	nsubj	expression,depends
R3918	T6003	T6001	prep	of,expression
R3919	T6004	T6003	pobj	Sepw1,of
R392	T1038	T1029	punct	", ",denote
R3920	T6005	T6002	prep	on,depends
R3921	T6006	T6005	pobj	MTF,on
R3922	T6007	T6006	punct	-,MTF
R3923	T6008	T6006	nummod	1,MTF
R3924	T6010	T6011	nsubjpass	Sepw1,found
R3925	T6012	T6011	auxpass	was,found
R3926	T6013	T6011	prep	in,found
R3927	T6014	T6015	compound	microarray,analysis
R3928	T6015	T6013	pobj	analysis,in
R3929	T6016	T6017	aux	to,be
R393	T1039	T1029	advmod	respectively,denote
R3930	T6017	T6011	xcomp	be,found
R3931	T6018	T6017	advmod	significantly,be
R3932	T6019	T6017	acomp	downregulated,be
R3933	T6020	T6017	prep	in,be
R3934	T6021	T6020	pobj	livers,in
R3935	T6022	T6021	prep	from,livers
R3936	T6023	T6024	npadvmod	cadmium,treated
R3937	T6024	T6029	amod	treated,mice
R3938	T6025	T6023	punct	-,cadmium
R3939	T6026	T6023	cc	and,cadmium
R394	T1040	T1014	punct	),used
R3940	T6027	T6023	conj	mock,cadmium
R3941	T6028	T6024	punct	-,treated
R3942	T6029	T6022	pobj	mice,from
R3943	T6030	T6031	compound	Mtf1Mx,cre
R3944	T6031	T6029	compound	cre,mice
R3945	T6032	T6031	punct	-,cre
R3946	T6033	T6034	punct	(,a
R3947	T6034	T6011	parataxis	a,found
R3948	T6035	T6034	dep	Table,a
R3949	T6036	T6035	nummod	1,Table
R395	T1041	T997	punct	.,triggered
R3950	T6037	T6034	punct	", ",a
R3951	T6038	T6034	cc	and,a
R3952	T6039	T6034	conj	c,a
R3953	T6040	T6034	punct	),a
R3954	T6041	T6011	punct	.,found
R3955	T6043	T6044	nsubj	SEPW1,is
R3956	T6045	T6046	det	a,protein
R3957	T6046	T6044	attr	protein,is
R3958	T6047	T6048	npadvmod	selenocysteine,containing
R3959	T6048	T6046	amod	containing,protein
R396	T1043	T1044	nsubj	Metallothioneins,play
R3960	T6049	T6048	punct	-,containing
R3961	T6050	T6051	dep	that,binds
R3962	T6051	T6046	relcl	binds,protein
R3963	T6052	T6051	dobj	glutathione,binds
R3964	T6053	T6054	punct	(,35
R3965	T6054	T6051	parataxis	35,binds
R3966	T6055	T6054	punct	),35
R3967	T6056	T6051	cc	and,binds
R3968	T6057	T6058	auxpass	is,thought
R3969	T6058	T6051	conj	thought,binds
R397	T1045	T1043	punct	(,Metallothioneins
R3970	T6059	T6060	aux	to,act
R3971	T6060	T6058	xcomp	act,thought
R3972	T6061	T6060	prep	as,act
R3973	T6062	T6063	det	an,antioxidant
R3974	T6063	T6061	pobj	antioxidant,as
R3975	T6064	T6065	advmod	in,vivo
R3976	T6065	T6060	advmod	vivo,act
R3977	T6066	T6067	punct	(,36
R3978	T6067	T6058	parataxis	36,thought
R3979	T6068	T6067	punct	),36
R398	T1046	T1043	appos	MTs,Metallothioneins
R3980	T6069	T6044	punct	.,is
R3981	T6071	T6072	compound	Sepw1,expression
R3982	T6072	T6073	nsubjpass	expression,analyzed
R3983	T6074	T6072	prep	in,expression
R3984	T6075	T6074	pobj	livers,in
R3985	T6076	T6075	prep	of,livers
R3986	T6077	T6078	compound	pI,pC
R3987	T6078	T6080	npadvmod	pC,induced
R3988	T6079	T6078	punct	–,pC
R3989	T6080	T6082	amod	induced,mice
R399	T1047	T1043	punct	),Metallothioneins
R3990	T6081	T6080	punct	-,induced
R3991	T6082	T6076	pobj	mice,of
R3992	T6083	T6082	punct	", ",mice
R3993	T6084	T6085	amod	mock,treated
R3994	T6085	T6082	amod	treated,mice
R3995	T6086	T6084	punct	-,mock
R3996	T6087	T6084	cc	or,mock
R3997	T6088	T6084	conj	cadmium,mock
R3998	T6089	T6085	punct	-,treated
R3999	T6090	T6091	nmod	Mtf1Mx,cre
R40	T278	T277	prep	to,response
R400	T1048	T1043	punct	", ",Metallothioneins
R4000	T6091	T6082	nmod	cre,mice
R4001	T6092	T6091	punct	-,cre
R4002	T6093	T6091	cc	and,cre
R4003	T6094	T6091	conj	Mtf1loxP,cre
R4004	T6095	T6073	auxpass	was,analyzed
R4005	T6096	T6073	advmod	further,analyzed
R4006	T6097	T6073	prep	by,analyzed
R4007	T6098	T6099	amod	semiquantitative,analysis
R4008	T6099	T6097	pobj	analysis,by
R4009	T6100	T6101	nmod	RT,PCRs
R401	T1049	T1050	amod	small,proteins
R4010	T6101	T6099	nmod	PCRs,analysis
R4011	T6102	T6101	punct	–,PCRs
R4012	T6103	T6101	cc	and,PCRs
R4013	T6104	T6101	conj	S1,PCRs
R4014	T6105	T6106	punct	(,2a
R4015	T6106	T6073	parataxis	2a,analyzed
R4016	T6107	T6106	compound	Figure,2a
R4017	T6108	T6106	cc	and,2a
R4018	T6109	T6106	conj	b,2a
R4019	T6110	T6106	punct	),2a
R402	T1050	T1043	appos	proteins,Metallothioneins
R4020	T6111	T6073	punct	.,analyzed
R4021	T6113	T6114	prep	In,observed
R4022	T6115	T6113	pobj	accordance,In
R4023	T6116	T6115	prep	with,accordance
R4024	T6117	T6118	compound	microarray,data
R4025	T6118	T6116	pobj	data,with
R4026	T6119	T6120	det	a,upregulation
R4027	T6120	T6114	nsubjpass	upregulation,observed
R4028	T6121	T6120	amod	slight,upregulation
R4029	T6122	T6123	punct	", ",any
R403	T1051	T1050	punct	", ",proteins
R4030	T6123	T6121	parataxis	any,slight
R4031	T6124	T6123	mark	if,any
R4032	T6125	T6123	punct	", ",any
R4033	T6126	T6120	prep	of,upregulation
R4034	T6127	T6128	compound	Sepw1,transcription
R4035	T6128	T6126	pobj	transcription,of
R4036	T6129	T6114	auxpass	was,observed
R4037	T6130	T6114	prep	in,observed
R4038	T6131	T6130	pobj	livers,in
R4039	T6132	T6131	prep	from,livers
R404	T1052	T1053	npadvmod	cysteine,rich
R4040	T6133	T6134	compound	Mtf1loxP,mice
R4041	T6134	T6132	pobj	mice,from
R4042	T6135	T6114	prep	upon,observed
R4043	T6136	T6137	compound	cadmium,treatment
R4044	T6137	T6135	pobj	treatment,upon
R4045	T6138	T6114	punct	.,observed
R4046	T6140	T6141	det	The,level
R4047	T6141	T6143	nsubjpass	level,reduced
R4048	T6142	T6141	amod	basal,level
R4049	T6144	T6143	auxpass	was,reduced
R405	T1053	T1050	amod	rich,proteins
R4050	T6145	T6143	prep	in,reduced
R4051	T6146	T6145	pobj	livers,in
R4052	T6147	T6146	prep	from,livers
R4053	T6148	T6149	amod	mock,treated
R4054	T6149	T6154	amod	treated,mice
R4055	T6150	T6148	punct	-,mock
R4056	T6151	T6148	cc	and,mock
R4057	T6152	T6148	conj	cadmium,mock
R4058	T6153	T6149	punct	-,treated
R4059	T6154	T6147	pobj	mice,from
R406	T1054	T1053	punct	-,rich
R4060	T6155	T6156	compound	Mtf1Mx,cre
R4061	T6156	T6154	compound	cre,mice
R4062	T6157	T6156	punct	-,cre
R4063	T6158	T6143	punct	", ",reduced
R4064	T6159	T6143	advcl	indicating,reduced
R4065	T6160	T6161	mark	that,is
R4066	T6161	T6159	ccomp	is,indicating
R4067	T6162	T6161	nsubj	MTF,is
R4068	T6163	T6162	punct	-,MTF
R4069	T6164	T6162	nummod	1,MTF
R407	T1055	T1044	punct	", ",play
R4070	T6165	T6161	acomp	important,is
R4071	T6166	T6165	prep	for,important
R4072	T6167	T6168	det	the,expression
R4073	T6168	T6166	pobj	expression,for
R4074	T6169	T6168	amod	basal,expression
R4075	T6170	T6168	prep	of,expression
R4076	T6171	T6170	pobj	Sepw1,of
R4077	T6172	T6143	punct	.,reduced
R4078	T6174	T6175	nummod	Three,sequences
R4079	T6175	T6179	nsubjpass	sequences,found
R408	T1056	T1057	det	an,role
R4080	T6176	T6175	compound	MRE,sequences
R4081	T6177	T6178	compound	core,consensus
R4082	T6178	T6175	compound	consensus,sequences
R4083	T6180	T6179	auxpass	were,found
R4084	T6181	T6179	prep	in,found
R4085	T6182	T6183	det	the,region
R4086	T6183	T6181	pobj	region,in
R4087	T6184	T6183	advmod	upstream,region
R4088	T6185	T6184	prep	of,upstream
R4089	T6186	T6187	det	the,start
R409	T1057	T1044	dobj	role,play
R4090	T6187	T6185	pobj	start,of
R4091	T6188	T6189	compound	mouse,Sepw1
R4092	T6189	T6187	compound	Sepw1,start
R4093	T6190	T6187	compound	transcription,start
R4094	T6191	T6192	punct	(,2c
R4095	T6192	T6179	parataxis	2c,found
R4096	T6193	T6192	compound	Figure,2c
R4097	T6194	T6192	punct	),2c
R4098	T6195	T6179	punct	.,found
R4099	T6197	T6198	nsubj	Two,overlap
R41	T279	T280	amod	various,conditions
R410	T1058	T1057	amod	important,role
R4100	T6198	T6204	ccomp	overlap,located
R4101	T6199	T6197	prep	of,Two
R4102	T6200	T6199	pobj	them,of
R4103	T6201	T6197	prep	in,Two
R4104	T6202	T6203	amod	opposite,orientation
R4105	T6203	T6201	pobj	orientation,in
R4106	T6205	T6206	advmod	almost,completely
R4107	T6206	T6207	advmod	completely,proximal
R4108	T6207	T6198	advmod	proximal,overlap
R4109	T6208	T6207	prep	to,proximal
R411	T1059	T1044	prep	in,play
R4110	T6209	T6210	det	the,start
R4111	T6210	T6208	pobj	start,to
R4112	T6211	T6210	compound	transcription,start
R4113	T6212	T6213	punct	(,bp
R4114	T6213	T6198	parataxis	bp,overlap
R4115	T6214	T6213	dep	MRE1,bp
R4116	T6215	T6213	punct	", ",bp
R4117	T6216	T6217	punct	−,40
R4118	T6217	T6213	nummod	40,bp
R4119	T6218	T6213	punct	),bp
R412	T1060	T1061	compound	metal,homeostasis
R4120	T6219	T6204	punct	", ",located
R4121	T6220	T6221	det	the,one
R4122	T6221	T6204	nsubjpass	one,located
R4123	T6222	T6221	amod	third,one
R4124	T6223	T6204	auxpass	is,located
R4125	T6224	T6225	advmod	further,upstream
R4126	T6225	T6204	advmod	upstream,located
R4127	T6226	T6227	punct	(,bp
R4128	T6227	T6204	parataxis	bp,located
R4129	T6228	T6227	dep	MRE2,bp
R413	T1061	T1059	pobj	homeostasis,in
R4130	T6229	T6227	punct	", ",bp
R4131	T6230	T6231	punct	−,527
R4132	T6231	T6227	nummod	527,bp
R4133	T6232	T6227	punct	),bp
R4134	T6233	T6204	punct	.,located
R4135	T6235	T6236	amod	Specific,binding
R4136	T6236	T6237	nsubjpass	binding,observed
R4137	T6238	T6236	prep	of,binding
R4138	T6239	T6238	pobj	MTF,of
R4139	T6240	T6239	punct	-,MTF
R414	T1062	T1061	cc	and,homeostasis
R4140	T6241	T6239	nummod	1,MTF
R4141	T6242	T6236	prep	to,binding
R4142	T6243	T6244	nmod	Sepw1,oligonucleotide
R4143	T6244	T6242	pobj	oligonucleotide,to
R4144	T6245	T6244	nmod	MRE1,oligonucleotide
R4145	T6246	T6245	cc	but,MRE1
R4146	T6247	T6246	neg	not,but
R4147	T6248	T6245	conj	MRE2,MRE1
R4148	T6249	T6237	auxpass	was,observed
R4149	T6250	T6237	prep	in,observed
R415	T1063	T1061	conj	detoxification,homeostasis
R4150	T6251	T6250	pobj	EMSA,in
R4151	T6252	T6251	prep	with,EMSA
R4152	T6253	T6254	compound	liver,protein
R4153	T6254	T6255	compound	protein,extract
R4154	T6255	T6252	pobj	extract,with
R4155	T6256	T6255	prep	from,extract
R4156	T6257	T6258	det	an,mouse
R4157	T6258	T6256	pobj	mouse,from
R4158	T6259	T6258	compound	Mtf1loxP,mouse
R4159	T6260	T6258	compound	control,mouse
R416	T1064	T1044	prep	due,play
R4160	T6261	T6262	punct	(,2d
R4161	T6262	T6237	parataxis	2d,observed
R4162	T6263	T6262	compound	Figure,2d
R4163	T6264	T6262	punct	),2d
R4164	T6265	T6237	punct	.,observed
R4165	T6267	T6268	prep	As,detected
R4166	T6269	T6270	det	a,control
R4167	T6270	T6267	pobj	control,As
R4168	T6271	T6268	punct	", ",detected
R4169	T6272	T6273	det	no,binding
R417	T1065	T1064	pcomp	to,due
R4170	T6273	T6268	nsubjpass	binding,detected
R4171	T6274	T6273	prep	to,binding
R4172	T6275	T6274	pobj	MRE1,to
R4173	T6276	T6268	auxpass	was,detected
R4174	T6277	T6268	prep	with,detected
R4175	T6278	T6277	pobj	extract,with
R4176	T6279	T6278	prep	from,extract
R4177	T6280	T6281	det	a,mouse
R4178	T6281	T6279	pobj	mouse,from
R4179	T6282	T6283	compound	pI,pC
R418	T1066	T1067	poss	their,ability
R4180	T6283	T6285	npadvmod	pC,induced
R4181	T6284	T6283	punct	–,pC
R4182	T6285	T6281	amod	induced,mouse
R4183	T6286	T6285	punct	-,induced
R4184	T6287	T6288	compound	Mtf1Mx,cre
R4185	T6288	T6281	compound	cre,mouse
R4186	T6289	T6288	punct	-,cre
R4187	T6290	T6268	punct	", ",detected
R4188	T6291	T6268	advcl	confirming,detected
R4189	T6292	T6293	mark	that,was
R419	T1067	T1064	pobj	ability,due
R4190	T6293	T6291	ccomp	was,confirming
R4191	T6294	T6295	det	the,bandshift
R4192	T6295	T6293	nsubj	bandshift,was
R4193	T6296	T6293	advmod	indeed,was
R4194	T6297	T6293	acomp	dependent,was
R4195	T6298	T6297	prep	on,dependent
R4196	T6299	T6300	det	the,presence
R4197	T6300	T6298	pobj	presence,on
R4198	T6301	T6300	prep	of,presence
R4199	T6302	T6301	pobj	MTF,of
R42	T280	T278	pobj	conditions,to
R420	T1068	T1069	aux	to,bind
R4200	T6303	T6302	punct	-,MTF
R4201	T6304	T6302	nummod	1,MTF
R4202	T6305	T6268	punct	.,detected
R4203	T6587	T6588	compound	Cadmium,response
R4204	T6588	T6589	nsubj	response,depends
R4205	T6590	T6588	prep	of,response
R4206	T6591	T6590	pobj	Ndrg1,of
R4207	T6592	T6589	prep	on,depends
R4208	T6593	T6592	pobj	MTF,on
R4209	T6594	T6593	punct	-,MTF
R421	T1069	T1067	acl	bind,ability
R4210	T6595	T6593	nummod	1,MTF
R4211	T6597	T6598	nsubjpass	Ndrg1,downregulated
R4212	T6599	T6598	auxpass	was,downregulated
R4213	T6600	T6598	advmod	significantly,downregulated
R4214	T6601	T6598	prep	in,downregulated
R4215	T6602	T6601	pobj	microarrays,in
R4216	T6603	T6602	prep	of,microarrays
R4217	T6604	T6605	compound	liver,transcripts
R4218	T6605	T6603	pobj	transcripts,of
R4219	T6606	T6605	prep	from,transcripts
R422	T1070	T1071	amod	different,ions
R4220	T6607	T6608	npadvmod	cadmium,treated
R4221	T6608	T6610	amod	treated,mice
R4222	T6609	T6608	punct	-,treated
R4223	T6610	T6606	pobj	mice,from
R4224	T6611	T6612	compound	Mtf1Mx,cre
R4225	T6612	T6610	compound	cre,mice
R4226	T6613	T6612	punct	-,cre
R4227	T6614	T6598	prep	compared,downregulated
R4228	T6615	T6614	prep	to,compared
R4229	T6616	T6617	advmod	similarly,treated
R423	T1071	T1069	dobj	ions,bind
R4230	T6617	T6618	amod	treated,mice
R4231	T6618	T6615	pobj	mice,to
R4232	T6619	T6618	compound	Mtf1loxP,mice
R4233	T6620	T6618	compound	control,mice
R4234	T6621	T6622	punct	(,c
R4235	T6622	T6598	parataxis	c,downregulated
R4236	T6623	T6622	nmod	Table,c
R4237	T6624	T6622	nummod	1,c
R4238	T6625	T6622	punct	", ",c
R4239	T6626	T6622	punct	),c
R424	T1072	T1073	amod	heavy,metal
R4240	T6627	T6598	punct	.,downregulated
R4241	T6629	T6630	nsubj	Ndrg1,has
R4242	T6631	T6630	advmod	probably,has
R4243	T6632	T6633	det	some,role
R4244	T6633	T6630	dobj	role,has
R4245	T6634	T6633	prep	in,role
R4246	T6635	T6636	compound	stress,response
R4247	T6636	T6634	pobj	response,in
R4248	T6637	T6638	mark	since,activate
R4249	T6638	T6630	advcl	activate,has
R425	T1073	T1071	compound	metal,ions
R4250	T6639	T6640	amod	various,stimuli
R4251	T6640	T6638	nsubj	stimuli,activate
R4252	T6641	T6640	punct	", ",stimuli
R4253	T6642	T6640	prep	including,stimuli
R4254	T6643	T6644	nmod	hypoxia,compounds
R4255	T6644	T6642	pobj	compounds,including
R4256	T6645	T6643	cc	and,hypoxia
R4257	T6646	T6643	conj	nickel,hypoxia
R4258	T6647	T6638	punct	", ",activate
R4259	T6648	T6638	dobj	expression,activate
R426	T1074	T1075	punct	(,1
R4260	T6649	T6648	prep	of,expression
R4261	T6650	T6651	compound	rodent,Ndrg1
R4262	T6651	T6649	pobj	Ndrg1,of
R4263	T6652	T6648	cc	and,expression
R4264	T6653	T6652	punct	/,and
R4265	T6654	T6652	cc	or,and
R4266	T6655	T6656	poss	its,ortholog
R4267	T6656	T6648	conj	ortholog,expression
R4268	T6657	T6656	amod	human,ortholog
R4269	T6658	T6659	punct	(,37
R427	T1075	T1067	parataxis	1,ability
R4270	T6659	T6638	parataxis	37,activate
R4271	T6660	T6661	punct	–,40
R4272	T6661	T6659	prep	40,37
R4273	T6662	T6659	punct	),37
R4274	T6663	T6630	punct	.,has
R4275	T6665	T6666	det	The,results
R4276	T6666	T6669	nsubjpass	results,confirmed
R4277	T6667	T6666	compound	Ndrg1,results
R4278	T6668	T6666	compound	microarray,results
R4279	T6669	T6671	ccomp	confirmed,was
R428	T1076	T1077	punct	–,3
R4280	T6670	T6669	auxpass	were,confirmed
R4281	T6672	T6669	prep	with,confirmed
R4282	T6673	T6674	amod	semiquantitative,PCRs
R4283	T6674	T6672	pobj	PCRs,with
R4284	T6675	T6674	compound	RT,PCRs
R4285	T6676	T6674	punct	–,PCRs
R4286	T6677	T6678	punct	(,3a
R4287	T6678	T6669	parataxis	3a,confirmed
R4288	T6679	T6678	compound	Figure,3a
R4289	T6680	T6678	punct	),3a
R429	T1077	T1075	prep	3,1
R4290	T6681	T6671	punct	: ,was
R4291	T6682	T6683	prep	for,observed
R4292	T6683	T6671	ccomp	observed,was
R4293	T6684	T6685	compound	Mtf1loxP,livers
R4294	T6685	T6682	pobj	livers,for
R4295	T6686	T6685	compound	control,livers
R4296	T6687	T6683	punct	", ",observed
R4297	T6688	T6689	det	a,increase
R4298	T6689	T6683	nsubjpass	increase,observed
R4299	T6690	T6689	amod	clear,increase
R43	T281	T280	compound	stress,conditions
R430	T1078	T1075	punct	),1
R4300	T6691	T6689	prep	of,increase
R4301	T6692	T6693	compound	Ndrg1,expression
R4302	T6693	T6691	pobj	expression,of
R4303	T6694	T6683	auxpass	was,observed
R4304	T6695	T6683	prep	after,observed
R4305	T6696	T6697	compound	cadmium,exposure
R4306	T6697	T6695	pobj	exposure,after
R4307	T6698	T6671	punct	;,was
R4308	T6699	T6671	prep	in,was
R4309	T6700	T6699	pobj	livers,in
R431	T1079	T1044	punct	.,play
R4310	T6701	T6700	prep	from,livers
R4311	T6702	T6703	compound	Mtf1Mx,cre
R4312	T6703	T6705	compound	cre,mice
R4313	T6704	T6703	punct	-,cre
R4314	T6705	T6701	pobj	mice,from
R4315	T6706	T6671	punct	", ",was
R4316	T6707	T6708	det	this,response
R4317	T6708	T6671	nsubj	response,was
R4318	T6709	T6708	compound	cadmium,response
R4319	T6710	T6671	neg	not,was
R432	T1081	T1082	prep	In,are
R4320	T6711	T6671	acomp	detectable,was
R4321	T6712	T6671	punct	", ",was
R4322	T6713	T6714	mark	while,was
R4323	T6714	T6671	advcl	was,was
R4324	T6715	T6716	amod	basal,expression
R4325	T6716	T6714	nsubj	expression,was
R4326	T6717	T6714	acomp	similar,was
R4327	T6718	T6717	prep	to,similar
R4328	T6719	T6718	pobj	controls,to
R4329	T6720	T6671	punct	.,was
R433	T1083	T1084	det	the,mouse
R4330	T6722	T6723	nsubj	This,indicates
R4331	T6724	T6725	mark	that,depends
R4332	T6725	T6723	ccomp	depends,indicates
R4333	T6726	T6727	npadvmod	cadmium,induced
R4334	T6727	T6729	amod	induced,expression
R4335	T6728	T6727	punct	-,induced
R4336	T6729	T6725	nsubj	expression,depends
R4337	T6730	T6729	prep	of,expression
R4338	T6731	T6730	pobj	Ndrg1,of
R4339	T6732	T6725	prep	on,depends
R434	T1084	T1081	pobj	mouse,In
R4340	T6733	T6732	pobj	MTF,on
R4341	T6734	T6733	punct	-,MTF
R4342	T6735	T6733	nummod	1,MTF
R4343	T6736	T6723	punct	.,indicates
R4344	T6738	T6739	nummod	Five,sequences
R4345	T6739	T6743	nsubjpass	sequences,located
R4346	T6740	T6739	compound	MRE,sequences
R4347	T6741	T6739	compound	core,sequences
R4348	T6742	T6739	compound	consensus,sequences
R4349	T6744	T6743	auxpass	are,located
R435	T1085	T1082	punct	", ",are
R4350	T6745	T6743	advmod	upstream,located
R4351	T6746	T6745	prep	of,upstream
R4352	T6747	T6748	det	the,start
R4353	T6748	T6746	pobj	start,of
R4354	T6749	T6748	compound	mouse,start
R4355	T6750	T6748	compound	Ndrg1,start
R4356	T6751	T6748	compound	transcription,start
R4357	T6752	T6753	punct	(,3b
R4358	T6753	T6743	parataxis	3b,located
R4359	T6754	T6753	compound	Figure,3b
R436	T1086	T1082	expl	there,are
R4360	T6755	T6753	punct	),3b
R4361	T6756	T6743	punct	.,located
R4362	T6758	T6759	nsubjpass	Four,clustered
R4363	T6759	T6763	ccomp	clustered,located
R4364	T6760	T6758	prep	of,Four
R4365	T6761	T6760	pobj	them,of
R4366	T6762	T6759	auxpass	are,clustered
R4367	T6764	T6765	punct	(,bp
R4368	T6765	T6759	parataxis	bp,clustered
R4369	T6766	T6765	dep	MRE1,bp
R437	T1087	T1088	nummod	four,genes
R4370	T6767	T6766	prep	to,MRE1
R4371	T6768	T6767	pobj	MRE4,to
R4372	T6769	T6765	punct	", ",bp
R4373	T6770	T6771	punct	−,332
R4374	T6771	T6765	nummod	332,bp
R4375	T6772	T6771	quantmod	138,332
R4376	T6773	T6771	quantmod	to,332
R4377	T6774	T6771	punct	−,332
R4378	T6775	T6765	punct	),bp
R4379	T6776	T6763	punct	", ",located
R438	T1088	T1082	attr	genes,are
R4380	T6777	T6778	det	the,one
R4381	T6778	T6763	nsubjpass	one,located
R4382	T6779	T6778	amod	fifth,one
R4383	T6780	T6763	auxpass	is,located
R4384	T6781	T6782	advmod	farther,upstream
R4385	T6782	T6763	advmod	upstream,located
R4386	T6783	T6784	punct	(,bp
R4387	T6784	T6763	parataxis	bp,located
R4388	T6785	T6784	dep	MRE5,bp
R4389	T6786	T6784	punct	", ",bp
R439	T1089	T1088	compound	metallothionein,genes
R4390	T6787	T6788	punct	−,883
R4391	T6788	T6784	nummod	883,bp
R4392	T6789	T6784	punct	),bp
R4393	T6790	T6763	punct	.,located
R4394	T6792	T6793	nsubjpass	EMSA,performed
R4395	T6794	T6793	auxpass	was,performed
R4396	T6795	T6796	aux	to,test
R4397	T6796	T6793	advcl	test,performed
R4398	T6797	T6798	mark	whether,interacting
R4399	T6798	T6796	ccomp	interacting,test
R44	T282	T280	punct	", ",conditions
R440	T1090	T1088	punct	", ",genes
R4400	T6799	T6798	nsubj	MTF,interacting
R4401	T6800	T6799	punct	-,MTF
R4402	T6801	T6799	nummod	1,MTF
R4403	T6802	T6798	aux	is,interacting
R4404	T6803	T6798	prep	with,interacting
R4405	T6804	T6803	pobj	some,with
R4406	T6805	T6804	cc	or,some
R4407	T6806	T6804	conj	all,some
R4408	T6807	T6806	prep	of,all
R4409	T6808	T6809	det	the,sequences
R441	T1091	T1088	acl	designated,genes
R4410	T6809	T6807	pobj	sequences,of
R4411	T6810	T6809	nummod	four,sequences
R4412	T6811	T6809	amod	proximal,sequences
R4413	T6812	T6809	compound	MRE,sequences
R4414	T6813	T6814	punct	(,3c
R4415	T6814	T6798	parataxis	3c,interacting
R4416	T6815	T6814	compound	Figure,3c
R4417	T6816	T6814	punct	),3c
R4418	T6817	T6793	punct	.,performed
R4419	T6819	T6820	amod	Separate,oligonucleotides
R442	T1092	T1091	prep	as,designated
R4420	T6820	T6821	nsubjpass	oligonucleotides,tested
R4421	T6822	T6821	auxpass	were,tested
R4422	T6823	T6821	prep	for,tested
R4423	T6824	T6823	pobj	MRE1,for
R4424	T6825	T6824	cc	and,MRE1
R4425	T6826	T6824	conj	MRE2,MRE1
R4426	T6827	T6821	punct	", ",tested
R4427	T6828	T6829	mark	whereas,used
R4428	T6829	T6821	advcl	used,tested
R4429	T6830	T6831	nummod	one,oligonucleotide
R443	T1093	T1092	pobj	Mt1,as
R4430	T6831	T6829	nsubjpass	oligonucleotide,used
R4431	T6832	T6831	acl	spanning,oligonucleotide
R4432	T6833	T6834	det	both,sequences
R4433	T6834	T6832	dobj	sequences,spanning
R4434	T6835	T6829	auxpass	was,used
R4435	T6836	T6829	prep	for,used
R4436	T6837	T6836	pobj	MRE3,for
R4437	T6838	T6837	cc	and,MRE3
R4438	T6839	T6837	conj	MRE4,MRE3
R4439	T6840	T6841	punct	(,4
R444	T1094	T1093	prep	to,Mt1
R4440	T6841	T6829	parataxis	4,used
R4441	T6842	T6841	dep	MRE3,4
R4442	T6843	T6841	punct	",",4
R4443	T6844	T6841	punct	),4
R4444	T6845	T6821	punct	.,tested
R4445	T6847	T6848	det	No,complex
R4446	T6848	T6849	nsubjpass	complex,seen
R4447	T6850	T6849	auxpass	was,seen
R4448	T6851	T6849	prep	with,seen
R4449	T6852	T6851	pobj	MRE1,with
R445	T1095	T1094	pobj	Mt4,to
R4450	T6853	T6849	punct	", ",seen
R4451	T6854	T6849	cc	but,seen
R4452	T6855	T6856	amod	specific,complexes
R4453	T6856	T6860	nsubjpass	complexes,observed
R4454	T6857	T6856	nmod	MTF,complexes
R4455	T6858	T6857	punct	-,MTF
R4456	T6859	T6857	nummod	1,MTF
R4457	T6860	T6849	conj	observed,seen
R4458	T6861	T6860	auxpass	were,observed
R4459	T6862	T6860	prep	for,observed
R446	T1096	T1082	punct	.,are
R4460	T6863	T6864	preconj	both,MRE2
R4461	T6864	T6862	pobj	MRE2,for
R4462	T6865	T6864	det	the,MRE2
R4463	T6866	T6864	cc	and,MRE2
R4464	T6867	T6864	conj	MRE3,MRE2
R4465	T6868	T6864	punct	",",MRE2
R4466	T6869	T6870	nummod	4,oligonucleotides
R4467	T6870	T6864	appos	oligonucleotides,MRE2
R4468	T6871	T6870	prep	with,oligonucleotides
R4469	T6872	T6873	compound	liver,extract
R447	T1098	T1099	amod	Basal,expression
R4470	T6873	T6871	pobj	extract,with
R4471	T6874	T6873	compound	protein,extract
R4472	T6875	T6873	prep	from,extract
R4473	T6876	T6877	det	an,mouse
R4474	T6877	T6875	pobj	mouse,from
R4475	T6878	T6877	compound	Mtf1loxP,mouse
R4476	T6879	T6860	punct	.,observed
R4477	T6881	T6882	mark	As,expected
R4478	T6882	T6883	advcl	expected,observed
R4479	T6884	T6883	punct	", ",observed
R448	T1099	T1109	nsubjpass	expression,mediated
R4480	T6885	T6886	det	no,bandshift
R4481	T6886	T6883	nsubjpass	bandshift,observed
R4482	T6887	T6883	auxpass	was,observed
R4483	T6888	T6883	prep	with,observed
R4484	T6889	T6890	compound	protein,extract
R4485	T6890	T6888	pobj	extract,with
R4486	T6891	T6890	prep	from,extract
R4487	T6892	T6893	det	a,mouse
R4488	T6893	T6891	pobj	mouse,from
R4489	T6894	T6893	acl	lacking,mouse
R449	T1100	T1098	punct	", ",Basal
R4490	T6895	T6894	dobj	MTF,lacking
R4491	T6896	T6895	punct	-,MTF
R4492	T6897	T6895	nummod	1,MTF
R4493	T6898	T6893	punct	(,mouse
R4494	T6899	T6900	compound	Mtf1Mx,cre
R4495	T6900	T6893	appos	cre,mouse
R4496	T6901	T6900	punct	-,cre
R4497	T6902	T6883	punct	),observed
R4498	T6903	T6883	punct	.,observed
R4499	T6934	T6962	punct	-,cre
R45	T283	T284	advmod	notably,load
R450	T1101	T1102	advmod	as,as
R4500	T6935	T6985	pobj	proteins,of
R4501	T6936	T6937	compound	Cadmium,response
R4502	T6937	T6938	nsubj	response,depends
R4503	T6939	T6937	prep	of,response
R4504	T6940	T6939	pobj	Csrp1,of
R4505	T6941	T6938	prep	on,depends
R4506	T6942	T6941	pobj	MTF,on
R4507	T6943	T6942	punct	-,MTF
R4508	T6944	T6942	nummod	1,MTF
R4509	T6946	T6947	nsubjpass	Csrp1,found
R451	T1102	T1098	cc	as,Basal
R4510	T6948	T6947	auxpass	was,found
R4511	T6949	T6947	prep	in,found
R4512	T6950	T6951	compound	microarray,analyses
R4513	T6951	T6949	pobj	analyses,in
R4514	T6952	T6953	aux	to,downregulated
R4515	T6953	T6947	xcomp	downregulated,found
R4516	T6954	T6953	auxpass	be,downregulated
R4517	T6955	T6953	advmod	significantly,downregulated
R4518	T6956	T6953	prep	in,downregulated
R4519	T6957	T6958	npadvmod	cadmium,treated
R452	T1103	T1102	advmod	well,as
R4520	T6958	T6960	amod	treated,mice
R4521	T6959	T6958	punct	-,treated
R4522	T6960	T6956	pobj	mice,in
R4523	T6961	T6962	compound	Mtf1Mx,cre
R4524	T6962	T6960	compound	cre,mice
R4525	T6963	T6953	prep	compared,downregulated
R4526	T6964	T6963	prep	to,compared
R4527	T6965	T6966	compound	Mtf1loxP,mice
R4528	T6966	T6964	pobj	mice,to
R4529	T6967	T6968	punct	(,c
R453	T1104	T1105	amod	heavy,metal
R4530	T6968	T6953	parataxis	c,downregulated
R4531	T6969	T6968	nmod	Table,c
R4532	T6970	T6968	nummod	1,c
R4533	T6971	T6968	punct	", ",c
R4534	T6972	T6968	punct	),c
R4535	T6973	T6947	punct	.,found
R4536	T6975	T6976	nsubj	CSRP1,is
R4537	T6977	T6978	det	a,member
R4538	T6978	T6976	attr	member,is
R4539	T6979	T6978	prep	of,member
R454	T1105	T1106	npadvmod	metal,induced
R4540	T6980	T6981	det	the,family
R4541	T6981	T6979	pobj	family,of
R4542	T6982	T6983	amod	evolutionary,conserved
R4543	T6983	T6981	amod	conserved,family
R4544	T6984	T6981	compound	CRP,family
R4545	T6985	T6981	prep	of,family
R4546	T6986	T6987	dep	that,implicated
R4547	T6987	T6935	relcl	implicated,proteins
R4548	T6988	T6987	aux	have,implicated
R4549	T6989	T6987	auxpass	been,implicated
R455	T1106	T1098	conj	induced,Basal
R4550	T6990	T6987	prep	in,implicated
R4551	T6991	T6990	pobj	myogenesis,in
R4552	T6992	T6991	cc	and,myogenesis
R4553	T6993	T6994	amod	cytoskeletal,remodeling
R4554	T6994	T6991	conj	remodeling,myogenesis
R4555	T6995	T6996	punct	(,42
R4556	T6996	T6987	parataxis	42,implicated
R4557	T6997	T6996	nummod	41,42
R4558	T6998	T6996	punct	",",42
R4559	T6999	T6996	punct	),42
R456	T1107	T1106	punct	-,induced
R4560	T7000	T6976	punct	.,is
R4561	T7002	T7003	amod	Semiquantitative,PCRs
R4562	T7003	T7006	nsubj	PCRs,confirmed
R4563	T7004	T7003	compound	RT,PCRs
R4564	T7005	T7003	punct	–,PCRs
R4565	T7007	T7008	det	the,microarray
R4566	T7008	T7006	dobj	microarray,confirmed
R4567	T7009	T7008	nmod	results,microarray
R4568	T7010	T7008	punct	", ",microarray
R4569	T7011	T7012	advmod	namely,elevated
R457	T1108	T1099	punct	", ",expression
R4570	T7012	T7008	acl	elevated,microarray
R4571	T7013	T7012	punct	", ",elevated
R4572	T7014	T7012	mark	that,elevated
R4573	T7015	T7016	compound	Csrp1,expression
R4574	T7016	T7012	nsubjpass	expression,elevated
R4575	T7017	T7012	auxpass	is,elevated
R4576	T7018	T7012	prep	in,elevated
R4577	T7019	T7020	compound	Mtf1loxP,livers
R4578	T7020	T7018	pobj	livers,in
R4579	T7021	T7012	prep	upon,elevated
R458	T1110	T1099	prep	of,expression
R4580	T7022	T7023	compound	cadmium,exposure
R4581	T7023	T7021	pobj	exposure,upon
R4582	T7024	T7025	punct	(,4a
R4583	T7025	T7012	parataxis	4a,elevated
R4584	T7026	T7025	compound	Figure,4a
R4585	T7027	T7025	punct	),4a
R4586	T7028	T7006	punct	.,confirmed
R4587	T7030	T7031	prep	In,was
R4588	T7032	T7030	pobj	contrast,In
R4589	T7033	T7031	punct	", ",was
R459	T1111	T1110	pobj	Mt1,of
R4590	T7034	T7035	det	no,response
R4591	T7035	T7031	nsubj	response,was
R4592	T7036	T7035	compound	cadmium,response
R4593	T7037	T7031	acomp	detectable,was
R4594	T7038	T7031	prep	in,was
R4595	T7039	T7038	pobj	livers,in
R4596	T7040	T7039	prep	from,livers
R4597	T7041	T7042	compound	Mtf1Mx,cre
R4598	T7042	T7044	compound	cre,mice
R4599	T7043	T7042	punct	-,cre
R46	T284	T280	appos	load,conditions
R460	T1112	T1111	cc	and,Mt1
R4600	T7044	T7040	pobj	mice,from
R4601	T7045	T7031	punct	", ",was
R4602	T7046	T7031	advcl	suggesting,was
R4603	T7047	T7048	mark	that,required
R4604	T7048	T7046	ccomp	required,suggesting
R4605	T7049	T7048	nsubjpass	MTF,required
R4606	T7050	T7049	punct	-,MTF
R4607	T7051	T7049	nummod	1,MTF
R4608	T7052	T7048	auxpass	is,required
R4609	T7053	T7048	prep	for,required
R461	T1113	T1111	conj	Mt2,Mt1
R4610	T7054	T7055	compound	cadmium,induction
R4611	T7055	T7053	pobj	induction,for
R4612	T7056	T7055	prep	of,induction
R4613	T7057	T7056	pobj	Csrp1,of
R4614	T7058	T7031	punct	.,was
R4615	T7060	T7061	nummod	Three,sequences
R4616	T7061	T7065	nsubjpass	sequences,found
R4617	T7062	T7061	compound	MRE,sequences
R4618	T7063	T7064	compound	core,consensus
R4619	T7064	T7061	compound	consensus,sequences
R462	T1114	T1109	auxpass	is,mediated
R4620	T7065	T7067	ccomp	found,found
R4621	T7066	T7065	auxpass	were,found
R4622	T7068	T7065	advmod	upstream,found
R4623	T7069	T7068	prep	of,upstream
R4624	T7070	T7071	det	the,start
R4625	T7071	T7069	pobj	start,of
R4626	T7072	T7073	compound	Csrp1,transcription
R4627	T7073	T7071	compound	transcription,start
R4628	T7074	T7075	punct	(,bp
R4629	T7075	T7071	parataxis	bp,start
R463	T1115	T1109	agent	by,mediated
R4630	T7076	T7075	dep	MRE2,bp
R4631	T7077	T7076	prep	to,MRE2
R4632	T7078	T7077	pobj	MRE4,to
R4633	T7079	T7075	punct	", ",bp
R4634	T7080	T7081	punct	−,56
R4635	T7081	T7082	quantmod	56,366
R4636	T7082	T7075	nummod	366,bp
R4637	T7083	T7082	quantmod	to,366
R4638	T7084	T7082	punct	−,366
R4639	T7085	T7075	punct	),bp
R464	T1116	T1117	npadvmod	metal,responsive
R4640	T7086	T7067	punct	", ",found
R4641	T7087	T7067	nsubjpass	one,found
R4642	T7088	T7067	auxpass	was,found
R4643	T7089	T7090	advmod	immediately,downstream
R4644	T7090	T7067	advmod	downstream,found
R4645	T7091	T7092	punct	(,4b
R4646	T7092	T7067	parataxis	4b,found
R4647	T7093	T7092	dep	MRE1,4b
R4648	T7094	T7093	punct	", ",MRE1
R4649	T7095	T7096	punct	+,7
R465	T1117	T1119	amod	responsive,factor
R4650	T7096	T7097	nummod	7,bp
R4651	T7097	T7093	appos	bp,MRE1
R4652	T7098	T7092	punct	;,4b
R4653	T7099	T7092	compound	Figure,4b
R4654	T7100	T7092	punct	),4b
R4655	T7101	T7067	punct	.,found
R4656	T7103	T7104	amod	Specific,binding
R4657	T7104	T7105	nsubjpass	binding,observed
R4658	T7106	T7104	prep	of,binding
R4659	T7107	T7106	pobj	MTF,of
R466	T1118	T1117	punct	-,responsive
R4660	T7108	T7107	punct	-,MTF
R4661	T7109	T7107	nummod	1,MTF
R4662	T7110	T7105	auxpass	was,observed
R4663	T7111	T7105	prep	with,observed
R4664	T7112	T7111	pobj	EMSA,with
R4665	T7113	T7112	prep	for,EMSA
R4666	T7114	T7115	compound	MRE2,oligonucleotide
R4667	T7115	T7113	pobj	oligonucleotide,for
R4668	T7116	T7115	cc	and,oligonucleotide
R4669	T7117	T7118	compound	protein,extract
R467	T1119	T1115	pobj	factor,by
R4670	T7118	T7115	conj	extract,oligonucleotide
R4671	T7119	T7118	prep	from,extract
R4672	T7120	T7121	det	an,liver
R4673	T7121	T7123	nmod	liver,extract
R4674	T7122	T7121	nmod	Mtf1loxP,liver
R4675	T7123	T7119	pobj	extract,from
R4676	T7124	T7121	punct	", ",liver
R4677	T7125	T7121	cc	but,liver
R4678	T7126	T7125	neg	not,but
R4679	T7127	T7128	det	an,liver
R468	T1120	T1119	compound	transcription,factor
R4680	T7128	T7121	conj	liver,liver
R4681	T7129	T7130	compound	Mtf1Mx,cre
R4682	T7130	T7128	compound	cre,liver
R4683	T7131	T7130	punct	-,cre
R4684	T7132	T7123	acl	lacking,extract
R4685	T7133	T7132	dobj	MTF,lacking
R4686	T7134	T7133	punct	-,MTF
R4687	T7135	T7133	nummod	1,MTF
R4688	T7136	T7105	punct	", ",observed
R4689	T7137	T7105	advcl	confirming,observed
R469	T1121	T1119	nummod	1,factor
R4690	T7138	T7139	det	the,participation
R4691	T7139	T7137	dobj	participation,confirming
R4692	T7140	T7139	prep	of,participation
R4693	T7141	T7140	pobj	MTF,of
R4694	T7142	T7141	punct	-,MTF
R4695	T7143	T7141	nummod	1,MTF
R4696	T7144	T7139	prep	in,participation
R4697	T7145	T7146	det	the,complex
R4698	T7146	T7144	pobj	complex,in
R4699	T7147	T7148	punct	(,4c
R47	T285	T286	amod	heavy,metal
R470	T1122	T1119	punct	(,factor
R4700	T7148	T7137	parataxis	4c,confirming
R4701	T7149	T7148	compound	Figure,4c
R4702	T7150	T7148	punct	),4c
R4703	T7151	T7105	punct	.,observed
R4706	T7421	T7422	nsubj	MTF,inhibits
R4707	T7423	T7421	punct	-,MTF
R4708	T7424	T7421	nummod	1,MTF
R4709	T7425	T7422	dobj	expression,inhibits
R471	T1123	T1119	appos	MTF,factor
R4710	T7426	T7425	prep	of,expression
R4711	T7427	T7426	pobj	Slc39a10,of
R4712	T7429	T7430	nsubjpass	Slc39a10,detected
R4713	T7431	T7430	auxpass	was,detected
R4714	T7432	T7430	prep	in,detected
R4715	T7433	T7434	compound	microarray,analysis
R4716	T7434	T7432	pobj	analysis,in
R4717	T7435	T7436	aux	to,upregulated
R4718	T7436	T7430	xcomp	upregulated,detected
R4719	T7437	T7436	auxpass	be,upregulated
R472	T1124	T1123	punct	-,MTF
R4720	T7438	T7436	advmod	significantly,upregulated
R4721	T7439	T7436	prep	in,upregulated
R4722	T7440	T7439	pobj	livers,in
R4723	T7441	T7440	prep	from,livers
R4724	T7442	T7443	preconj	both,mock
R4725	T7443	T7444	amod	mock,treated
R4726	T7444	T7449	amod	treated,mice
R4727	T7445	T7443	punct	-,mock
R4728	T7446	T7443	cc	and,mock
R4729	T7447	T7443	conj	cadmium,mock
R473	T1125	T1123	nummod	1,MTF
R4730	T7448	T7444	punct	-,treated
R4731	T7449	T7441	pobj	mice,from
R4732	T7450	T7451	compound	Mtf1Mx,cre
R4733	T7451	T7449	compound	cre,mice
R4734	T7452	T7451	punct	-,cre
R4735	T7453	T7436	prep	compared,upregulated
R4736	T7454	T7453	prep	to,compared
R4737	T7455	T7456	compound	control,animals
R4738	T7456	T7454	pobj	animals,to
R4739	T7457	T7458	punct	(,b
R474	T1126	T1109	punct	),mediated
R4740	T7458	T7456	parataxis	b,animals
R4741	T7459	T7458	dep	Table,b
R4742	T7460	T7459	nummod	1,Table
R4743	T7461	T7458	punct	", ",b
R4744	T7462	T7458	cc	and,b
R4745	T7463	T7458	conj	d,b
R4746	T7464	T7458	punct	),b
R4747	T7465	T7430	punct	.,detected
R4748	T7467	T7468	compound	SLC39,proteins
R4749	T7468	T7469	nsubj	proteins,are
R475	T1127	T1128	punct	(,4
R4750	T7470	T7469	attr	members,are
R4751	T7471	T7470	prep	of,members
R4752	T7472	T7473	det	the,family
R4753	T7473	T7471	pobj	family,of
R4754	T7474	T7475	npadvmod	Zrt,like
R4755	T7475	T7480	amod	like,protein
R4756	T7476	T7474	punct	-,Zrt
R4757	T7477	T7474	cc	and,Zrt
R4758	T7478	T7474	conj	Irt,Zrt
R4759	T7479	T7475	punct	-,like
R476	T1128	T1109	parataxis	4,mediated
R4760	T7480	T7473	nmod	protein,family
R4761	T7481	T7480	punct	(,protein
R4762	T7482	T7480	appos	ZIP,protein
R4763	T7483	T7473	punct	),family
R4764	T7484	T7473	prep	of,family
R4765	T7485	T7486	compound	metal,transporters
R4766	T7486	T7484	pobj	transporters,of
R4767	T7487	T7486	compound	ion,transporters
R4768	T7488	T7489	dep	that,transport
R4769	T7489	T7473	relcl	transport,family
R477	T1129	T1130	punct	–,7
R4770	T7490	T7489	punct	", ",transport
R4771	T7491	T7489	prep	with,transport
R4772	T7492	T7493	det	no,exception
R4773	T7493	T7491	pobj	exception,with
R4774	T7494	T7493	amod	known,exception
R4775	T7495	T7489	punct	", ",transport
R4776	T7496	T7497	compound	metal,ion
R4777	T7497	T7498	compound	ion,substrates
R4778	T7498	T7489	dobj	substrates,transport
R4779	T7499	T7489	prep	across,transport
R478	T1130	T1128	prep	7,4
R4780	T7500	T7501	amod	cellular,membranes
R4781	T7501	T7499	pobj	membranes,across
R4782	T7502	T7489	prep	into,transport
R4783	T7503	T7504	det	the,cytoplasm
R4784	T7504	T7502	pobj	cytoplasm,into
R4785	T7505	T7506	punct	(,44
R4786	T7506	T7469	parataxis	44,are
R4787	T7507	T7506	nummod	43,44
R4788	T7508	T7506	punct	",",44
R4789	T7509	T7506	punct	),44
R479	T1131	T1128	punct	),4
R4790	T7510	T7469	punct	.,are
R4791	T7512	T7513	prep	In,showed
R4792	T7514	T7512	pobj	accordance,In
R4793	T7515	T7514	prep	with,accordance
R4794	T7516	T7517	compound	microarray,data
R4795	T7517	T7515	pobj	data,with
R4796	T7518	T7513	punct	", ",showed
R4797	T7519	T7520	amod	semiquantitative,PCRs
R4798	T7520	T7513	nsubj	PCRs,showed
R4799	T7521	T7520	compound	RT,PCRs
R48	T286	T284	compound	metal,load
R480	T1132	T1109	punct	.,mediated
R4800	T7522	T7520	punct	–,PCRs
R4801	T7523	T7524	det	a,downregulation
R4802	T7524	T7513	dobj	downregulation,showed
R4803	T7525	T7524	prep	of,downregulation
R4804	T7526	T7527	compound	Slc39a10,expression
R4805	T7527	T7525	pobj	expression,of
R4806	T7528	T7513	prep	in,showed
R4807	T7529	T7528	pobj	livers,in
R4808	T7530	T7529	prep	of,livers
R4809	T7531	T7532	compound	Mtf1loxP,mice
R481	T1134	T1135	det	This,protein
R4810	T7532	T7530	pobj	mice,of
R4811	T7533	T7513	prep	upon,showed
R4812	T7534	T7535	compound	cadmium,exposure
R4813	T7535	T7533	pobj	exposure,upon
R4814	T7536	T7513	punct	.,showed
R4815	T7538	T7539	prep	In,increased
R4816	T7539	T7552	ccomp	increased,resulted
R4817	T7540	T7538	pobj	samples,In
R4818	T7541	T7540	prep	from,samples
R4819	T7542	T7543	compound	Mtf1Mx,cre
R482	T1135	T1138	nsubj	protein,recognizes
R4820	T7543	T7545	compound	cre,mice
R4821	T7544	T7543	punct	-,cre
R4822	T7545	T7541	pobj	mice,from
R4823	T7546	T7539	punct	", ",increased
R4824	T7547	T7548	det	the,expression
R4825	T7548	T7539	nsubjpass	expression,increased
R4826	T7549	T7548	amod	basal,expression
R4827	T7550	T7539	auxpass	was,increased
R4828	T7551	T7539	advmod	significantly,increased
R4829	T7553	T7552	punct	;,resulted
R483	T1136	T1135	compound	zinc,protein
R4830	T7554	T7555	compound	cadmium,treatment
R4831	T7555	T7552	nsubj	treatment,resulted
R4832	T7556	T7552	advmod	still,resulted
R4833	T7557	T7552	prep	in,resulted
R4834	T7558	T7559	det	a,decrease
R4835	T7559	T7557	pobj	decrease,in
R4836	T7560	T7559	prep	of,decrease
R4837	T7561	T7562	compound	Slc39a10,expression
R4838	T7562	T7560	pobj	expression,of
R4839	T7563	T7564	punct	(,5a
R484	T1137	T1135	compound	finger,protein
R4840	T7564	T7552	parataxis	5a,resulted
R4841	T7565	T7564	compound	Figure,5a
R4842	T7566	T7564	punct	),5a
R4843	T7567	T7552	punct	.,resulted
R4844	T7569	T7570	nsubjpass	It,judged
R4845	T7571	T7570	aux	can,judged
R4846	T7572	T7570	neg	not,judged
R4847	T7573	T7570	auxpass	be,judged
R4848	T7574	T7570	prep	by,judged
R4849	T7575	T7576	det	this,experiment
R485	T1139	T1140	amod	short,sequences
R4850	T7576	T7574	pobj	experiment,by
R4851	T7577	T7578	mark	whether,was
R4852	T7578	T7570	ccomp	was,judged
R4853	T7579	T7580	det	the,degree
R4854	T7580	T7578	nsubj	degree,was
R4855	T7581	T7580	prep	of,degree
R4856	T7582	T7583	npadvmod	cadmium,induced
R4857	T7583	T7585	amod	induced,reduction
R4858	T7584	T7583	punct	-,induced
R4859	T7585	T7581	pobj	reduction,of
R486	T1140	T1138	dobj	sequences,recognizes
R4860	T7586	T7585	prep	of,reduction
R4861	T7587	T7588	compound	Slc39a10,transcription
R4862	T7588	T7586	pobj	transcription,of
R4863	T7589	T7578	acomp	identical,was
R4864	T7590	T7589	prep	for,identical
R4865	T7591	T7592	nmod	Mtf1Mx,cre
R4866	T7592	T7594	nmod	cre,mice
R4867	T7593	T7592	punct	-,cre
R4868	T7594	T7590	pobj	mice,for
R4869	T7595	T7592	cc	and,cre
R487	T1141	T1142	npadvmod	cis,acting
R4870	T7596	T7592	conj	Mtf1loxP,cre
R4871	T7597	T7589	cc	or,identical
R4872	T7598	T7589	conj	lower,identical
R4873	T7599	T7598	prep	in,lower
R4874	T7600	T7601	det	the,absence
R4875	T7601	T7599	pobj	absence,in
R4876	T7602	T7601	prep	of,absence
R4877	T7603	T7602	pobj	MTF,of
R4878	T7604	T7603	punct	-,MTF
R4879	T7605	T7603	nummod	1,MTF
R488	T1142	T1140	amod	acting,sequences
R4880	T7606	T7570	punct	.,judged
R4881	T7608	T7609	prep	In,was
R4882	T7610	T7611	compound	microarray,analysis
R4883	T7611	T7608	pobj	analysis,In
R4884	T7612	T7609	punct	", ",was
R4885	T7613	T7614	det	the,degree
R4886	T7614	T7609	nsubj	degree,was
R4887	T7615	T7614	prep	of,degree
R4888	T7616	T7617	det	the,downregulation
R4889	T7617	T7615	pobj	downregulation,of
R489	T1143	T1142	punct	-,acting
R4890	T7618	T7619	preconj	either,comparable
R4891	T7619	T7609	acomp	comparable,was
R4892	T7620	T7619	prep	to,comparable
R4893	T7621	T7622	det	the,one
R4894	T7622	T7620	pobj	one,to
R4895	T7623	T7622	prep	in,one
R4896	T7624	T7625	compound	control,livers
R4897	T7625	T7623	pobj	livers,in
R4898	T7626	T7619	cc	or,comparable
R4899	T7627	T7619	conj	lower,comparable
R49	T287	T264	punct	", ",regulates
R490	T1144	T1140	compound	DNA,sequences
R4900	T7628	T7609	punct	", ",was
R4901	T7629	T7609	prep	depending,was
R4902	T7630	T7629	prep	on,depending
R4903	T7631	T7632	det	the,set
R4904	T7632	T7630	pobj	set,on
R4905	T7633	T7632	amod	considered,set
R4906	T7634	T7635	nmod	Affymetrix,GeneChip
R4907	T7635	T7632	nmod	GeneChip,set
R4908	T7636	T7635	punct	®,GeneChip
R4909	T7637	T7632	compound	probe,set
R491	T1145	T1140	punct	", ",sequences
R4910	T7638	T7639	punct	(,shown
R4911	T7639	T7609	parataxis	shown,was
R4912	T7640	T7639	nsubj	data,shown
R4913	T7641	T7639	neg	not,shown
R4914	T7642	T7639	punct	),shown
R4915	T7643	T7609	punct	.,was
R4916	T7645	T7646	det	The,results
R4917	T7646	T7647	nsubj	results,indicate
R4918	T7648	T7649	mark	that,involved
R4919	T7649	T7647	ccomp	involved,indicate
R492	T1146	T1140	acl	termed,sequences
R4920	T7650	T7649	nsubjpass	MTF,involved
R4921	T7651	T7650	punct	-,MTF
R4922	T7652	T7650	nummod	1,MTF
R4923	T7653	T7649	auxpass	is,involved
R4924	T7654	T7649	prep	in,involved
R4925	T7655	T7654	pobj	repression,in
R4926	T7656	T7655	prep	of,repression
R4927	T7657	T7658	det	the,expression
R4928	T7658	T7656	pobj	expression,of
R4929	T7659	T7658	amod	basal,expression
R493	T1147	T1148	compound	metal,elements
R4930	T7660	T7658	prep	of,expression
R4931	T7661	T7660	pobj	Slc39a10,of
R4932	T7662	T7647	punct	.,indicate
R4933	T7664	T7665	nsubj	It,participate
R4934	T7666	T7665	aux	might,participate
R4935	T7667	T7665	advmod	also,participate
R4936	T7668	T7665	prep	in,participate
R4937	T7669	T7670	det	the,response
R4938	T7670	T7668	pobj	response,in
R4939	T7671	T7670	compound	cadmium,response
R494	T1148	T1146	oprd	elements,termed
R4940	T7672	T7670	prep	of,response
R4941	T7673	T7674	det	this,gene
R4942	T7674	T7672	pobj	gene,of
R4943	T7675	T7665	punct	", ",participate
R4944	T7676	T7665	cc	but,participate
R4945	T7677	T7678	nsubj	it,is
R4946	T7678	T7665	conj	is,participate
R4947	T7679	T7678	advmod	apparently,is
R4948	T7680	T7678	neg	not,is
R4949	T7681	T7682	advmod	exclusively,responsible
R495	T1149	T1148	compound	response,elements
R4950	T7682	T7678	acomp	responsible,is
R4951	T7683	T7678	punct	.,is
R4952	T7685	T7686	nummod	One,sequence
R4953	T7686	T7690	nsubjpass	sequence,found
R4954	T7687	T7686	compound	MRE,sequence
R4955	T7688	T7689	compound	core,consensus
R4956	T7689	T7686	compound	consensus,sequence
R4957	T7690	T7692	ccomp	found,one
R4958	T7691	T7690	auxpass	was,found
R4959	T7693	T7694	advmod	just,upstream
R496	T1150	T1148	punct	(,elements
R4960	T7694	T7690	advmod	upstream,found
R4961	T7695	T7694	prep	of,upstream
R4962	T7696	T7697	det	the,start
R4963	T7697	T7695	pobj	start,of
R4964	T7698	T7697	compound	mouse,start
R4965	T7699	T7697	compound	Slc39a10,start
R4966	T7700	T7697	compound	transcription,start
R4967	T7701	T7702	punct	(,bp
R4968	T7702	T7690	parataxis	bp,found
R4969	T7703	T7702	dep	MRE1,bp
R497	T1151	T1148	appos	MREs,elements
R4970	T7704	T7702	punct	", ",bp
R4971	T7705	T7706	punct	−,21
R4972	T7706	T7702	nummod	21,bp
R4973	T7707	T7702	punct	),bp
R4974	T7708	T7692	punct	", ",one
R4975	T7709	T7692	det	another,one
R4976	T7710	T7711	advmod	directly,downstream
R4977	T7711	T7692	amod	downstream,one
R4978	T7712	T7713	punct	(,5b
R4979	T7713	T7692	parataxis	5b,one
R498	T1152	T1153	punct	;,TGCRCNC
R4980	T7714	T7713	dep	MRE2,5b
R4981	T7715	T7713	punct	", ",5b
R4982	T7716	T7717	punct	+,17
R4983	T7717	T7718	nummod	17,bp
R4984	T7718	T7713	dep	bp,5b
R4985	T7719	T7713	punct	;,5b
R4986	T7720	T7713	compound	Figure,5b
R4987	T7721	T7713	punct	),5b
R4988	T7722	T7692	punct	.,one
R4989	T7724	T7725	amod	Specific,binding
R499	T1153	T1146	parataxis	TGCRCNC,termed
R4990	T7725	T7726	nsubjpass	binding,observed
R4991	T7727	T7725	prep	of,binding
R4992	T7728	T7727	pobj	MTF,of
R4993	T7729	T7728	punct	-,MTF
R4994	T7730	T7728	nummod	1,MTF
R4995	T7731	T7726	auxpass	was,observed
R4996	T7732	T7726	prep	in,observed
R4997	T7733	T7734	compound	EMSA,analysis
R4998	T7734	T7732	pobj	analysis,in
R4999	T7735	T7734	prep	for,analysis
R50	T288	T264	prep	via,regulates
R500	T1154	T1153	compound	core,TGCRCNC
R5000	T7736	T7735	pobj	MRE2,for
R5001	T7737	T7734	prep	with,analysis
R5002	T7738	T7739	compound	liver,extract
R5003	T7739	T7737	pobj	extract,with
R5004	T7740	T7739	compound	protein,extract
R5005	T7741	T7739	prep	from,extract
R5006	T7742	T7743	det	an,Mtf1loxP
R5007	T7743	T7741	pobj	Mtf1loxP,from
R5008	T7744	T7741	cc	but,from
R5009	T7745	T7744	neg	not,but
R501	T1155	T1153	compound	consensus,TGCRCNC
R5010	T7746	T7741	conj	from,from
R5011	T7747	T7748	det	an,mouse
R5012	T7748	T7746	pobj	mouse,from
R5013	T7749	T7750	compound	Mtf1Mx,cre
R5014	T7750	T7748	compound	cre,mouse
R5015	T7751	T7750	punct	-,cre
R5016	T7752	T7726	punct	", ",observed
R5017	T7753	T7754	mark	while,detected
R5018	T7754	T7726	advcl	detected,observed
R5019	T7755	T7756	det	no,binding
R502	T1156	T1153	compound	sequence,TGCRCNC
R5020	T7756	T7754	nsubjpass	binding,detected
R5021	T7757	T7754	auxpass	was,detected
R5022	T7758	T7754	prep	with,detected
R5023	T7759	T7758	pobj	MRE1,with
R5024	T7760	T7761	punct	(,5c
R5025	T7761	T7754	parataxis	5c,detected
R5026	T7762	T7761	compound	Figure,5c
R5027	T7763	T7761	punct	),5c
R5028	T7764	T7726	punct	.,observed
R503	T1157	T1153	punct	),TGCRCNC
R5034	T8110	T8111	npadvmod	Cadmium,responsive
R5035	T8111	T8113	amod	responsive,genes
R5036	T8112	T8111	punct	-,responsive
R5037	T8114	T8113	punct	", ",genes
R5038	T8115	T8116	npadvmod	MTF,independent
R5039	T8116	T8113	amod	independent,genes
R504	T1158	T1140	punct	", ",sequences
R5040	T8117	T8115	punct	-,MTF
R5041	T8118	T8115	nummod	1,MTF
R5042	T8119	T8116	punct	-,independent
R5043	T8121	T8122	advmod	Finally,identified
R5044	T8123	T8122	punct	", ",identified
R5045	T8124	T8122	nsubj	we,identified
R5046	T8125	T8122	advmod	also,identified
R5047	T8126	T8127	det	a,number
R5048	T8127	T8122	dobj	number,identified
R5049	T8128	T8127	prep	of,number
R505	T1159	T1160	dep	which,are
R5050	T8129	T8130	npadvmod	cadmium,responsive
R5051	T8130	T8132	amod	responsive,genes
R5052	T8131	T8130	punct	-,responsive
R5053	T8132	T8128	pobj	genes,of
R5054	T8133	T8134	dep	that,were
R5055	T8134	T8127	relcl	were,number
R5056	T8135	T8134	acomp	independent,were
R5057	T8136	T8135	prep	of,independent
R5058	T8137	T8138	nmod	MTF,presence
R5059	T8138	T8136	pobj	presence,of
R506	T1160	T1140	relcl	are,sequences
R5060	T8139	T8137	punct	-,MTF
R5061	T8140	T8137	nummod	1,MTF
R5062	T8141	T8122	punct	", ",identified
R5063	T8142	T8122	prep	by,identified
R5064	T8143	T8142	pcomp	comparing,by
R5065	T8144	T8145	det	the,data
R5066	T8145	T8143	dobj	data,comparing
R5067	T8146	T8145	compound	probe,data
R5068	T8147	T8145	compound	array,data
R5069	T8148	T8145	prep	of,data
R507	T1161	T1160	acomp	present,are
R5070	T8149	T8150	det	all,mice
R5071	T8150	T8148	pobj	mice,of
R5072	T8151	T8152	npadvmod	cadmium,treated
R5073	T8152	T8150	amod	treated,mice
R5074	T8153	T8152	punct	-,treated
R5075	T8154	T8143	prep	with,comparing
R5076	T8155	T8156	det	the,data
R5077	T8156	T8154	pobj	data,with
R5078	T8157	T8156	prep	of,data
R5079	T8158	T8159	det	all,mice
R508	T1162	T1160	prep	in,are
R5080	T8159	T8157	pobj	mice,of
R5081	T8160	T8161	amod	mock,treated
R5082	T8161	T8159	amod	treated,mice
R5083	T8162	T8161	punct	-,treated
R5084	T8163	T8143	punct	", ",comparing
R5085	T8164	T8143	advmod	irrespective,comparing
R5086	T8165	T8164	prep	of,irrespective
R5087	T8166	T8167	det	the,genotype
R5088	T8167	T8165	pobj	genotype,of
R5089	T8168	T8169	punct	(,Table
R509	T1163	T1164	det	the,promoters
R5090	T8169	T8143	parataxis	Table,comparing
R5091	T8170	T8169	nummod	2,Table
R5092	T8171	T8169	punct	),Table
R5093	T8172	T8122	punct	.,identified
R5094	T8174	T8175	det	An,upregulation
R5095	T8175	T8181	nsubjpass	upregulation,observed
R5096	T8176	T8177	advmod	at,least
R5097	T8177	T8178	advmod	least,2-fold
R5098	T8178	T8175	amod	2-fold,upregulation
R5099	T8179	T8175	punct	", ",upregulation
R51	T289	T288	pobj	binding,via
R510	T1164	T1162	pobj	promoters,in
R5100	T8180	T8175	amod	reliable,upregulation
R5101	T8182	T8181	auxpass	was,observed
R5102	T8183	T8181	prep	after,observed
R5103	T8184	T8185	compound	cadmium,exposure
R5104	T8185	T8183	pobj	exposure,after
R5105	T8186	T8181	prep	for,observed
R5106	T8187	T8188	nummod	31,sets
R5107	T8188	T8186	pobj	sets,for
R5108	T8189	T8188	compound	probe,sets
R5109	T8190	T8188	acl	corresponding,sets
R511	T1165	T1164	prep	of,promoters
R5110	T8191	T8190	prep	to,corresponding
R5111	T8192	T8193	nummod	21,genes
R5112	T8193	T8191	pobj	genes,to
R5113	T8194	T8193	amod	different,genes
R5114	T8195	T8193	amod	characterized,genes
R5115	T8196	T8197	punct	(,a
R5116	T8197	T8181	parataxis	a,observed
R5117	T8198	T8197	nmod	Table,a
R5118	T8199	T8197	nummod	2,a
R5119	T8200	T8197	punct	", ",a
R512	T1166	T1167	compound	target,genes
R5120	T8201	T8197	punct	),a
R5121	T8202	T8181	punct	.,observed
R5122	T8204	T8205	prep	For,detected
R5123	T8206	T8207	nummod	2,sets
R5124	T8207	T8204	pobj	sets,For
R5125	T8208	T8207	compound	probe,sets
R5126	T8209	T8207	acl	corresponding,sets
R5127	T8210	T8209	prep	to,corresponding
R5128	T8211	T8212	nummod	2,genes
R5129	T8212	T8210	pobj	genes,to
R513	T1167	T1165	pobj	genes,of
R5130	T8213	T8212	amod	characterized,genes
R5131	T8214	T8205	punct	", ",detected
R5132	T8215	T8216	det	an,downregulation
R5133	T8216	T8205	nsubjpass	downregulation,detected
R5134	T8217	T8218	advmod	at,least
R5135	T8218	T8219	advmod	least,2-fold
R5136	T8219	T8216	amod	2-fold,downregulation
R5137	T8220	T8205	auxpass	was,detected
R5138	T8221	T8222	punct	(,b
R5139	T8222	T8205	parataxis	b,detected
R514	T1168	T1169	punct	(,9
R5140	T8223	T8222	nmod	Table,b
R5141	T8224	T8222	nummod	2,b
R5142	T8225	T8222	punct	", ",b
R5143	T8226	T8222	punct	),b
R5144	T8227	T8205	punct	.,detected
R5145	T8229	T8230	amod	Several,genes
R5146	T8230	T8231	nsubjpass	genes,found
R5147	T8232	T8230	acl	involved,genes
R5148	T8233	T8232	prep	in,involved
R5149	T8234	T8235	det	the,metabolism
R515	T1169	T1160	parataxis	9,are
R5150	T8235	T8233	pobj	metabolism,in
R5151	T8236	T8235	prep	of,metabolism
R5152	T8237	T8238	det	the,glutathione
R5153	T8238	T8236	pobj	glutathione,of
R5154	T8239	T8238	compound	antioxidant,glutathione
R5155	T8240	T8231	auxpass	were,found
R5156	T8241	T8242	aux	to,upregulated
R5157	T8242	T8231	xcomp	upregulated,found
R5158	T8243	T8242	auxpass	be,upregulated
R5159	T8244	T8242	agent	by,upregulated
R516	T1170	T1169	nummod	8,9
R5160	T8245	T8246	compound	cadmium,exposure
R5161	T8246	T8244	pobj	exposure,by
R5162	T8247	T8242	punct	", ",upregulated
R5163	T8248	T8249	advmod	namely,genes
R5164	T8249	T8242	dobj	genes,upregulated
R5165	T8250	T8249	det	the,genes
R5166	T8251	T8249	acl	encoding,genes
R5167	T8252	T8253	det	the,subunit
R5168	T8253	T8251	dobj	subunit,encoding
R5169	T8254	T8253	amod	catalytic,subunit
R517	T1171	T1169	punct	",",9
R5170	T8255	T8253	prep	of,subunit
R5171	T8256	T8257	compound	glutamate,ligase
R5172	T8257	T8255	pobj	ligase,of
R5173	T8258	T8257	punct	-,ligase
R5174	T8259	T8257	compound	cysteine,ligase
R5175	T8260	T8253	punct	(,subunit
R5176	T8261	T8253	appos	Gclc,subunit
R5177	T8262	T8253	punct	),subunit
R5178	T8263	T8264	dep	that,is
R5179	T8264	T8253	relcl	is,subunit
R518	T1172	T1169	punct	),9
R5180	T8265	T8266	det	the,enzyme
R5181	T8266	T8264	attr	enzyme,is
R5182	T8267	T8268	npadvmod	rate,limiting
R5183	T8268	T8266	amod	limiting,enzyme
R5184	T8269	T8266	prep	in,enzyme
R5185	T8270	T8271	advmod	de,novo
R5186	T8271	T8272	amod	novo,synthesis
R5187	T8272	T8269	pobj	synthesis,in
R5188	T8273	T8272	prep	of,synthesis
R5189	T8274	T8273	pobj	glutathione,of
R519	T1173	T1138	punct	.,recognizes
R5190	T8275	T8276	punct	(,45
R5191	T8276	T8264	parataxis	45,is
R5192	T8277	T8276	punct	),45
R5193	T8278	T8249	punct	;,genes
R5194	T8279	T8280	compound	glutathione,reductase
R5195	T8280	T8249	conj	reductase,genes
R5196	T8281	T8280	nummod	1,reductase
R5197	T8282	T8280	punct	(,reductase
R5198	T8283	T8280	appos	Gsr,reductase
R5199	T8284	T8280	punct	),reductase
R52	T290	T289	prep	to,binding
R520	T1175	T1176	nsubjpass	MTF,conserved
R5200	T8285	T8280	punct	", ",reductase
R5201	T8286	T8287	det	the,enzyme
R5202	T8287	T8280	appos	enzyme,reductase
R5203	T8288	T8287	amod	reducing,enzyme
R5204	T8289	T8287	prep	for,enzyme
R5205	T8290	T8291	amod	oxidized,glutathione
R5206	T8291	T8289	pobj	glutathione,for
R5207	T8292	T8293	punct	(,45
R5208	T8293	T8291	parataxis	45,glutathione
R5209	T8294	T8293	punct	),45
R521	T1177	T1175	punct	-,MTF
R5210	T8295	T8280	punct	;,reductase
R5211	T8296	T8280	cc	and,reductase
R5212	T8297	T8298	compound	glutathione,transferase
R5213	T8298	T8280	conj	transferase,reductase
R5214	T8299	T8298	punct	-,transferase
R5215	T8300	T8298	compound	S,transferase
R5216	T8301	T8298	punct	-,transferase
R5217	T8302	T8298	punct	", ",transferase
R5218	T8303	T8304	nmod	mu,Gstm4
R5219	T8304	T8298	appos	Gstm4,transferase
R522	T1178	T1175	nummod	1,MTF
R5220	T8305	T8304	nummod	4,Gstm4
R5221	T8306	T8304	punct	(,Gstm4
R5222	T8307	T8304	punct	),Gstm4
R5223	T8308	T8298	punct	", ",transferase
R5224	T8309	T8310	dep	which,is
R5225	T8310	T8298	relcl	is,transferase
R5226	T8311	T8312	det	a,member
R5227	T8312	T8310	attr	member,is
R5228	T8313	T8312	prep	of,member
R5229	T8314	T8315	det	the,family
R523	T1179	T1176	auxpass	is,conserved
R5230	T8315	T8313	pobj	family,of
R5231	T8316	T8317	compound	glutathione,transferase
R5232	T8317	T8315	compound	transferase,family
R5233	T8318	T8317	punct	-,transferase
R5234	T8319	T8317	compound	S,transferase
R5235	T8320	T8317	punct	-,transferase
R5236	T8321	T8315	compound	supergene,family
R5237	T8322	T8315	prep	of,family
R5238	T8323	T8324	compound	detoxification,enzymes
R5239	T8324	T8322	pobj	enzymes,of
R524	T1180	T1176	prep	in,conserved
R5240	T8325	T8326	punct	(,45
R5241	T8326	T8312	parataxis	45,member
R5242	T8327	T8326	punct	),45
R5243	T8328	T8231	punct	.,found
R5244	T8330	T8331	prep	In,confirmed
R5245	T8332	T8330	pobj	all,In
R5246	T8333	T8332	prep	of,all
R5247	T8334	T8335	det	these,cases
R5248	T8335	T8333	pobj	cases,of
R5249	T8336	T8331	punct	", ",confirmed
R525	T1181	T1180	pobj	evolution,in
R5250	T8337	T8331	nsubjpass	induction,confirmed
R5251	T8338	T8331	auxpass	was,confirmed
R5252	T8339	T8331	agent	by,confirmed
R5253	T8340	T8341	amod	semiquantitative,PCRs
R5254	T8341	T8339	pobj	PCRs,by
R5255	T8342	T8341	compound	RT,PCRs
R5256	T8343	T8341	punct	–,PCRs
R5257	T8344	T8345	punct	(,shown
R5258	T8345	T8331	parataxis	shown,confirmed
R5259	T8346	T8345	nsubj	data,shown
R526	T1182	T1176	punct	", ",conserved
R5260	T8347	T8345	neg	not,shown
R5261	T8348	T8345	punct	),shown
R5262	T8349	T8331	punct	.,confirmed
R5263	T8351	T8352	nsubjpass	Gclc,discussed
R5264	T8353	T8351	punct	", ",Gclc
R5265	T8354	T8355	advmod	also,referred
R5266	T8355	T8351	acl	referred,Gclc
R5267	T8356	T8355	prep	to,referred
R5268	T8357	T8355	prep	as,referred
R5269	T8358	T8359	amod	heavy,chain
R527	T1183	T1176	cc	and,conserved
R5270	T8359	T8360	compound	chain,subunit
R5271	T8360	T8357	pobj	subunit,as
R5272	T8361	T8360	prep	of,subunit
R5273	T8362	T8363	compound	gamma,glutamylcysteine
R5274	T8363	T8365	compound	glutamylcysteine,synthetase
R5275	T8364	T8363	punct	-,glutamylcysteine
R5276	T8365	T8361	pobj	synthetase,of
R5277	T8366	T8360	punct	(,subunit
R5278	T8367	T8368	compound	Ggcs,hc
R5279	T8368	T8360	appos	hc,subunit
R528	T1184	T1185	nsubjpass	homologs,characterized
R5280	T8369	T8368	punct	-,hc
R5281	T8370	T8352	punct	),discussed
R5282	T8371	T8352	punct	", ",discussed
R5283	T8372	T8352	aux	had,discussed
R5284	T8373	T8352	auxpass	been,discussed
R5285	T8374	T8352	advmod	previously,discussed
R5286	T8375	T8352	prep	as,discussed
R5287	T8376	T8377	det	a,gene
R5288	T8377	T8375	pobj	gene,as
R5289	T8378	T8377	compound	target,gene
R529	T1185	T1176	conj	characterized,conserved
R5290	T8379	T8377	prep	of,gene
R5291	T8380	T8379	pobj	MTF,of
R5292	T8381	T8380	punct	-,MTF
R5293	T8382	T8380	nummod	1,MTF
R5294	T8383	T8384	punct	(,6
R5295	T8384	T8352	parataxis	6,discussed
R5296	T8385	T8384	punct	),6
R5297	T8386	T8352	punct	.,discussed
R5298	T8388	T8389	poss	Our,data
R5299	T8389	T8391	nsubj	data,indicate
R53	T291	T292	compound	metal,elements
R530	T1186	T1185	aux	have,characterized
R5300	T8390	T8389	compound	expression,data
R5301	T8392	T8393	mark	that,induced
R5302	T8393	T8391	ccomp	induced,indicate
R5303	T8394	T8393	nsubjpass	Gclc,induced
R5304	T8395	T8393	auxpass	is,induced
R5305	T8396	T8393	agent	by,induced
R5306	T8397	T8396	pobj	cadmium,by
R5307	T8398	T8393	cc	but,induced
R5308	T8399	T8393	punct	", ",induced
R5309	T8400	T8401	advmod	at,least
R531	T1187	T1185	auxpass	been,characterized
R5310	T8401	T8402	advmod	least,in
R5311	T8402	T8403	prep	in,dependent
R5312	T8403	T8393	amod	dependent,induced
R5313	T8404	T8405	det	the,liver
R5314	T8405	T8402	pobj	liver,in
R5315	T8406	T8405	amod	adult,liver
R5316	T8407	T8405	compound	mouse,liver
R5317	T8408	T8403	punct	", ",dependent
R5318	T8409	T8403	neg	not,dependent
R5319	T8410	T8403	prep	on,dependent
R532	T1188	T1185	prep	in,characterized
R5320	T8411	T8410	pobj	MTF,on
R5321	T8412	T8411	punct	-,MTF
R5322	T8413	T8411	nummod	1,MTF
R5323	T8414	T8391	punct	.,indicate
R5324	T8416	T8417	aux	To,analyze
R5325	T8417	T8418	advcl	analyze,treated
R5326	T8419	T8420	det	the,role
R5327	T8420	T8417	dobj	role,analyze
R5328	T8421	T8420	prep	of,role
R5329	T8422	T8423	det	the,system
R533	T1189	T1190	det	the,mouse
R5330	T8423	T8421	pobj	system,of
R5331	T8424	T8423	compound	glutathione,system
R5332	T8425	T8420	prep	in,role
R5333	T8426	T8427	det	the,response
R5334	T8427	T8425	pobj	response,in
R5335	T8428	T8427	amod	cellular,response
R5336	T8429	T8427	compound	cadmium,response
R5337	T8430	T8418	punct	", ",treated
R5338	T8431	T8432	nmod	mouse,fibroblasts
R5339	T8432	T8418	nsubjpass	fibroblasts,treated
R534	T1190	T1188	pobj	mouse,in
R5340	T8433	T8432	amod	embryonic,fibroblasts
R5341	T8434	T8432	prep	with,fibroblasts
R5342	T8435	T8434	cc	and,with
R5343	T8436	T8434	conj	without,with
R5344	T8437	T8438	amod	functional,Mtf1
R5345	T8438	T8436	pobj	Mtf1,without
R5346	T8439	T8418	auxpass	were,treated
R5347	T8440	T8418	prep	with,treated
R5348	T8441	T8440	pobj	cadmium,with
R5349	T8442	T8418	prep	in,treated
R535	T1191	T1192	punct	(,10
R5350	T8443	T8442	pobj	combination,in
R5351	T8444	T8443	prep	with,combination
R5352	T8445	T8444	pobj	BSO,with
R5353	T8446	T8445	punct	", ",BSO
R5354	T8447	T8448	det	a,inhibitor
R5355	T8448	T8445	appos	inhibitor,BSO
R5356	T8449	T8448	amod	specific,inhibitor
R5357	T8450	T8448	prep	of,inhibitor
R5358	T8451	T8452	compound	glutamate,cysteine
R5359	T8452	T8454	compound	cysteine,ligase
R536	T1192	T1190	parataxis	10,mouse
R5360	T8453	T8452	punct	-,cysteine
R5361	T8454	T8450	pobj	ligase,of
R5362	T8455	T8456	punct	(,31
R5363	T8456	T8454	parataxis	31,ligase
R5364	T8457	T8456	punct	),31
R5365	T8458	T8418	punct	", ",treated
R5366	T8459	T8418	cc	and,treated
R5367	T8460	T8461	compound	cell,viability
R5368	T8461	T8462	nsubjpass	viability,assessed
R5369	T8462	T8418	conj	assessed,treated
R537	T1193	T1192	punct	),10
R5370	T8463	T8462	auxpass	was,assessed
R5371	T8464	T8462	agent	by,assessed
R5372	T8465	T8466	det	a,assay
R5373	T8466	T8464	pobj	assay,by
R5374	T8467	T8466	amod	colorimetric,assay
R5375	T8468	T8466	prep	based,assay
R5376	T8469	T8468	prep	on,based
R5377	T8470	T8471	det	the,salt
R5378	T8471	T8469	pobj	salt,on
R5379	T8472	T8471	compound	tetrazolium,salt
R538	T1194	T1190	punct	", ",mouse
R5380	T8473	T8471	appos	MTT,salt
R5381	T8474	T8475	punct	(,Figure
R5382	T8475	T8462	parataxis	Figure,assessed
R5383	T8476	T8475	nummod	6,Figure
R5384	T8477	T8475	punct	),Figure
R5385	T8478	T8462	punct	.,assessed
R5386	T8480	T8481	amod	Increasing,concentrations
R5387	T8481	T8482	nsubj	concentrations,were
R5388	T8483	T8481	prep	of,concentrations
R5389	T8484	T8483	pobj	BSO,of
R539	T1195	T1190	conj	humans,mouse
R5390	T8485	T8484	cc	or,BSO
R5391	T8486	T8484	conj	cadmium,BSO
R5392	T8487	T8484	advmod	alone,BSO
R5393	T8488	T8482	prep	to,were
R5394	T8489	T8490	det	some,extent
R5395	T8490	T8488	pobj	extent,to
R5396	T8491	T8482	acomp	cytotoxic,were
R5397	T8492	T8491	prep	for,cytotoxic
R5398	T8493	T8494	det	the,lines
R5399	T8494	T8492	pobj	lines,for
R54	T292	T290	pobj	elements,to
R540	T1196	T1197	punct	(,11
R5400	T8495	T8494	amod	examined,lines
R5401	T8496	T8494	compound	cell,lines
R5402	T8497	T8482	punct	.,were
R5403	T8499	T8500	nsubj	Treatment,resulted
R5404	T8501	T8499	prep	with,Treatment
R5405	T8502	T8503	preconj	both,BSO
R5406	T8503	T8501	pobj	BSO,with
R5407	T8504	T8503	cc	and,BSO
R5408	T8505	T8503	conj	cadmium,BSO
R5409	T8506	T8500	prep	in,resulted
R541	T1197	T1195	parataxis	11,humans
R5410	T8507	T8508	det	an,lethality
R5411	T8508	T8506	pobj	lethality,in
R5412	T8509	T8508	amod	enhanced,lethality
R5413	T8510	T8511	advmod	particularly,for
R5414	T8511	T8500	prep	for,resulted
R5415	T8512	T8513	det	the,cells
R5416	T8513	T8511	pobj	cells,for
R5417	T8514	T8513	prep	without,cells
R5418	T8515	T8516	amod	functional,Mtf1
R5419	T8516	T8514	pobj	Mtf1,without
R542	T1198	T1197	punct	),11
R5420	T8517	T8500	punct	", ",resulted
R5421	T8518	T8500	advcl	indicating,resulted
R5422	T8519	T8520	mark	that,impair
R5423	T8520	T8518	ccomp	impair,indicating
R5424	T8521	T8522	det	a,depletion
R5425	T8522	T8520	nsubj	depletion,impair
R5426	T8523	T8522	prep	of,depletion
R5427	T8524	T8523	pobj	glutathione,of
R5428	T8525	T8522	advmod	together,depletion
R5429	T8526	T8525	prep	with,together
R543	T1199	T1195	punct	", ",humans
R5430	T8527	T8528	det	a,lack
R5431	T8528	T8526	pobj	lack,with
R5432	T8529	T8528	prep	of,lack
R5433	T8530	T8529	pobj	Mtf1,of
R5434	T8531	T8532	det	an,defense
R5435	T8532	T8520	dobj	defense,impair
R5436	T8533	T8532	amod	efficient,defense
R5437	T8534	T8532	amod	anti-cadmium,defense
R5438	T8535	T8500	punct	.,resulted
R5439	T8537	T8538	advmod	Thus,are
R544	T1200	T1195	conj	Drosophila,humans
R5440	T8539	T8538	punct	", ",are
R5441	T8540	T8541	amod	adequate,supply
R5442	T8541	T8538	nsubj	supply,are
R5443	T8542	T8541	compound	glutathione,supply
R5444	T8543	T8544	advmod	as,as
R5445	T8544	T8541	cc	as,supply
R5446	T8545	T8544	advmod	well,as
R5447	T8546	T8541	conj	MTF,supply
R5448	T8547	T8546	punct	-,MTF
R5449	T8548	T8546	nummod	1,MTF
R545	T1201	T1202	punct	(,12
R5450	T8549	T8541	cc	and,supply
R5451	T8550	T8551	poss	its,genes
R5452	T8551	T8541	conj	genes,supply
R5453	T8552	T8551	compound	target,genes
R5454	T8553	T8538	acomp	essential,are
R5455	T8554	T8553	prep	for,essential
R5456	T8555	T8556	det	the,survival
R5457	T8556	T8554	pobj	survival,for
R5458	T8557	T8556	prep	of,survival
R5459	T8558	T8559	det	the,cell
R546	T1202	T1200	parataxis	12,Drosophila
R5460	T8559	T8557	pobj	cell,of
R5461	T8560	T8556	prep	under,survival
R5462	T8561	T8562	compound	cadmium,stress
R5463	T8562	T8560	pobj	stress,under
R5464	T8563	T8538	punct	.,are
R5465	T8565	T8566	prep	Besides,upregulated
R5466	T8567	T8565	pobj	genes,Besides
R5467	T8568	T8567	acl	related,genes
R5468	T8569	T8568	prep	to,related
R5469	T8570	T8571	det	the,pathway
R547	T1203	T1204	punct	–,14
R5470	T8571	T8569	pobj	pathway,to
R5471	T8572	T8571	compound	glutathione,pathway
R5472	T8573	T8566	punct	", ",upregulated
R5473	T8574	T8575	amod	several,genes
R5474	T8575	T8566	nsubjpass	genes,upregulated
R5475	T8576	T8575	amod	other,genes
R5476	T8577	T8578	npadvmod	stress,related
R5477	T8578	T8575	amod	related,genes
R5478	T8579	T8578	punct	-,related
R5479	T8580	T8566	auxpass	were,upregulated
R548	T1204	T1202	prep	14,12
R5480	T8581	T8566	prep	upon,upregulated
R5481	T8582	T8583	compound	cadmium,exposure
R5482	T8583	T8581	pobj	exposure,upon
R5483	T8584	T8566	punct	", ",upregulated
R5484	T8585	T8566	prep	including,upregulated
R5485	T8586	T8585	pobj	genes,including
R5486	T8587	T8586	prep	for,genes
R5487	T8588	T8589	compound	thioredoxin,reductase
R5488	T8589	T8587	pobj	reductase,for
R5489	T8590	T8589	nummod	1,reductase
R549	T1205	T1202	punct	),12
R5490	T8591	T8589	punct	(,reductase
R5491	T8592	T8589	appos	Txnrd1,reductase
R5492	T8593	T8589	punct	),reductase
R5493	T8594	T8589	punct	", ",reductase
R5494	T8595	T8589	appos	one,reductase
R5495	T8596	T8595	prep	of,one
R5496	T8597	T8598	det	the,enzymes
R5497	T8598	T8596	pobj	enzymes,of
R5498	T8599	T8598	amod	reducing,enzymes
R5499	T8600	T8595	prep	of,one
R55	T293	T292	compound	response,elements
R550	T1206	T1200	cc	and,Drosophila
R5500	T8601	T8602	det	the,thioredoxin
R5501	T8602	T8600	pobj	thioredoxin,of
R5502	T8603	T8602	compound	antioxidant,thioredoxin
R5503	T8604	T8605	punct	(,46
R5504	T8605	T8602	parataxis	46,thioredoxin
R5505	T8606	T8605	punct	),46
R5506	T8607	T8589	punct	;,reductase
R5507	T8608	T8609	nmod	KDEL,receptor
R5508	T8609	T8589	conj	receptor,reductase
R5509	T8610	T8609	amod	endoplasmic,receptor
R551	T1207	T1200	conj	fish,Drosophila
R5510	T8611	T8609	compound	reticulum,receptor
R5511	T8612	T8609	compound	protein,receptor
R5512	T8613	T8609	compound	retention,receptor
R5513	T8614	T8609	nummod	2,receptor
R5514	T8615	T8609	punct	(,receptor
R5515	T8616	T8609	appos	Kdelr2,receptor
R5516	T8617	T8609	punct	),receptor
R5517	T8618	T8609	acl	participating,receptor
R5518	T8619	T8618	prep	in,participating
R5519	T8620	T8621	compound	ER,response
R552	T1208	T1209	punct	(,16
R5520	T8621	T8619	pobj	response,in
R5521	T8622	T8621	compound	stress,response
R5522	T8623	T8624	punct	(,47
R5523	T8624	T8621	parataxis	47,response
R5524	T8625	T8624	punct	),47
R5525	T8626	T8609	punct	;,receptor
R5526	T8627	T8609	cc	and,receptor
R5527	T8628	T8629	det	the,athanogene
R5528	T8629	T8609	conj	athanogene,receptor
R5529	T8630	T8629	amod	anti-apoptotic,athanogene
R553	T1209	T1207	parataxis	16,fish
R5530	T8631	T8629	nmod	Bcl2,athanogene
R5531	T8632	T8629	punct	-,athanogene
R5532	T8633	T8629	amod	associated,athanogene
R5533	T8634	T8629	nummod	3,athanogene
R5534	T8635	T8629	punct	(,athanogene
R5535	T8636	T8629	appos	Bag3,athanogene
R5536	T8637	T8629	punct	),athanogene
R5537	T8638	T8629	acl	involved,athanogene
R5538	T8639	T8638	prep	in,involved
R5539	T8640	T8641	npadvmod	stress,induced
R554	T1210	T1209	nummod	15,16
R5540	T8641	T8643	amod	induced,apoptosis
R5541	T8642	T8641	punct	-,induced
R5542	T8643	T8639	pobj	apoptosis,in
R5543	T8644	T8645	punct	(,48
R5544	T8645	T8643	parataxis	48,apoptosis
R5545	T8646	T8645	punct	),48
R5546	T8647	T8566	punct	.,upregulated
R555	T1211	T1209	punct	",",16
R556	T1212	T1209	punct	),16
R5563	T9786	T9787	prep	In,affect
R5564	T9788	T9789	det	this,study
R5565	T9789	T9786	pobj	study,In
R5566	T9790	T9787	punct	", ",affect
R5567	T9791	T9792	det	a,deletion
R5568	T9792	T9787	nsubj	deletion,affect
R5569	T9793	T9794	advmod	virtually,complete
R557	T1213	T1185	punct	.,characterized
R5570	T9794	T9792	amod	complete,deletion
R5571	T9795	T9792	prep	of,deletion
R5572	T9796	T9795	pobj	Mtf1,of
R5573	T9797	T9792	prep	in,deletion
R5574	T9798	T9799	det	the,liver
R5575	T9799	T9797	pobj	liver,in
R5576	T9800	T9799	prep	of,liver
R5577	T9801	T9802	amod	adult,mice
R5578	T9802	T9800	pobj	mice,of
R5579	T9803	T9802	punct	", ",mice
R558	T1215	T1216	det	The,role
R5580	T9804	T9805	compound	pI,pC
R5581	T9805	T9807	npadvmod	pC,induced
R5582	T9806	T9805	punct	–,pC
R5583	T9807	T9802	amod	induced,mice
R5584	T9808	T9807	punct	-,induced
R5585	T9809	T9810	compound	Mtf1Mx,cre
R5586	T9810	T9802	compound	cre,mice
R5587	T9811	T9810	punct	-,cre
R5588	T9812	T9787	aux	did,affect
R5589	T9813	T9787	neg	not,affect
R559	T1216	T1217	nsubjpass	role,studied
R5590	T9814	T9787	advmod	detectably,affect
R5591	T9815	T9816	det	the,phenotype
R5592	T9816	T9787	dobj	phenotype,affect
R5593	T9817	T9816	prep	of,phenotype
R5594	T9818	T9819	det	the,mice
R5595	T9819	T9817	pobj	mice,of
R5596	T9820	T9819	amod	respective,mice
R5597	T9821	T9816	prep	under,phenotype
R5598	T9822	T9823	amod	non-stress,conditions
R5599	T9823	T9821	pobj	conditions,under
R56	T294	T292	punct	(,elements
R560	T1218	T1216	prep	of,role
R5600	T9824	T9787	punct	", ",affect
R5601	T9825	T9787	advcl	confirming,affect
R5602	T9826	T9827	mark	that,is
R5603	T9827	T9825	ccomp	is,confirming
R5604	T9828	T9827	nsubj	MTF,is
R5605	T9829	T9828	punct	-,MTF
R5606	T9830	T9828	nummod	1,MTF
R5607	T9831	T9827	acomp	dispensable,is
R5608	T9832	T9827	prep	in,is
R5609	T9833	T9834	det	the,liver
R561	T1219	T1218	pobj	MTF,of
R5610	T9834	T9832	pobj	liver,in
R5611	T9835	T9834	amod	adult,liver
R5612	T9836	T9837	punct	(,26
R5613	T9837	T9827	parataxis	26,is
R5614	T9838	T9837	punct	),26
R5615	T9839	T9787	punct	", ",affect
R5616	T9840	T9787	prep	in,affect
R5617	T9841	T9840	pobj	contrast,in
R5618	T9842	T9841	prep	to,contrast
R5619	T9843	T9844	poss	its,role
R562	T1220	T1219	punct	-,MTF
R5620	T9844	T9842	pobj	role,to
R5621	T9845	T9844	amod	essential,role
R5622	T9846	T9844	prep	in,role
R5623	T9847	T9848	amod	embryonic,development
R5624	T9848	T9846	pobj	development,in
R5625	T9849	T9848	compound	liver,development
R5626	T9850	T9851	punct	(,23
R5627	T9851	T9844	parataxis	23,role
R5628	T9852	T9851	punct	),23
R5629	T9853	T9787	punct	.,affect
R563	T1221	T1219	nummod	1,MTF
R5630	T9855	T9856	det	The,comparison
R5631	T9856	T9857	nsubj	comparison,revealed
R5632	T9858	T9856	prep	of,comparison
R5633	T9859	T9860	compound	gene,expression
R5634	T9860	T9858	pobj	expression,of
R5635	T9861	T9856	prep	in,comparison
R5636	T9862	T9861	pobj	livers,in
R5637	T9863	T9862	prep	of,livers
R5638	T9864	T9865	amod	mock,treated
R5639	T9865	T9870	amod	treated,mice
R564	T1222	T1217	aux	has,studied
R5640	T9866	T9864	punct	-,mock
R5641	T9867	T9864	cc	or,mock
R5642	T9868	T9864	conj	cadmium,mock
R5643	T9869	T9865	punct	-,treated
R5644	T9870	T9863	pobj	mice,of
R5645	T9871	T9872	nmod	Mtf1Mx,cre
R5646	T9872	T9870	nmod	cre,mice
R5647	T9873	T9872	punct	-,cre
R5648	T9874	T9872	cc	and,cre
R5649	T9875	T9872	conj	Mtf1loxP,cre
R565	T1223	T1217	auxpass	been,studied
R5650	T9876	T9877	amod	several,candidates
R5651	T9877	T9857	dobj	candidates,revealed
R5652	T9878	T9877	nmod	MTF,candidates
R5653	T9879	T9878	punct	-,MTF
R5654	T9880	T9878	nummod	1,MTF
R5655	T9881	T9877	compound	target,candidates
R5656	T9882	T9877	compound	gene,candidates
R5657	T9883	T9857	punct	.,revealed
R5658	T9885	T9886	nsubjpass	Transcripts,reduced
R5659	T9887	T9885	prep	of,Transcripts
R566	T1224	T1225	advmod	most,extensively
R5660	T9888	T9889	det	the,genes
R5661	T9889	T9887	pobj	genes,of
R5662	T9890	T9889	nummod	two,genes
R5663	T9891	T9892	npadvmod	stress,responsive
R5664	T9892	T9889	amod	responsive,genes
R5665	T9893	T9892	punct	-,responsive
R5666	T9894	T9889	compound	metallothionein,genes
R5667	T9895	T9889	appos	Mt1,genes
R5668	T9896	T9895	cc	and,Mt1
R5669	T9897	T9895	conj	Mt2,Mt1
R567	T1225	T1217	advmod	extensively,studied
R5670	T9898	T9886	auxpass	were,reduced
R5671	T9899	T9886	advmod	severely,reduced
R5672	T9900	T9886	punct	", ",reduced
R5673	T9901	T9886	prep	in,reduced
R5674	T9902	T9901	pobj	support,in
R5675	T9903	T9902	prep	of,support
R5676	T9904	T9905	det	a,role
R5677	T9905	T9903	pobj	role,of
R5678	T9906	T9905	amod	crucial,role
R5679	T9907	T9905	prep	of,role
R568	T1226	T1217	prep	in,studied
R5680	T9908	T9907	pobj	MTF,of
R5681	T9909	T9908	punct	-,MTF
R5682	T9910	T9908	nummod	1,MTF
R5683	T9911	T9905	prep	for,role
R5684	T9912	T9913	preconj	both,expression
R5685	T9913	T9911	pobj	expression,for
R5686	T9914	T9913	amod	basal,expression
R5687	T9915	T9914	cc	and,basal
R5688	T9916	T9917	npadvmod	metal,induced
R5689	T9917	T9914	conj	induced,basal
R569	T1227	T1228	det	the,mouse
R5690	T9918	T9917	punct	-,induced
R5691	T9919	T9913	prep	of,expression
R5692	T9920	T9919	pobj	metallothioneins,of
R5693	T9921	T9922	punct	(,6
R5694	T9922	T9886	parataxis	6,reduced
R5695	T9923	T9922	nummod	4,6
R5696	T9924	T9922	punct	",",6
R5697	T9925	T9922	punct	),6
R5698	T9926	T9886	punct	.,reduced
R5699	T9928	T9929	nsubj	One,is
R57	T295	T292	appos	MREs,elements
R570	T1228	T1226	pobj	mouse,in
R5700	T9930	T9928	prep	of,One
R5701	T9931	T9932	det	the,genes
R5702	T9932	T9930	pobj	genes,of
R5703	T9933	T9934	advmod	newly,found
R5704	T9934	T9932	amod	found,genes
R5705	T9935	T9932	compound	target,genes
R5706	T9936	T9929	attr	Sepw1,is
R5707	T9937	T9929	punct	.,is
R5708	T9939	T9940	det	The,function
R5709	T9940	T9943	nsubj	function,is
R571	T1229	T1217	punct	.,studied
R5710	T9941	T9940	amod	exact,function
R5711	T9942	T9940	amod	molecular,function
R5712	T9944	T9940	prep	of,function
R5713	T9945	T9946	compound	SEPW1,protein
R5714	T9946	T9944	pobj	protein,of
R5715	T9947	T9943	acomp	unknown,is
R5716	T9948	T9943	prep	to,is
R5717	T9949	T9948	pobj	date,to
R5718	T9950	T9943	punct	", ",is
R5719	T9951	T9943	cc	but,is
R572	T1231	T1232	prep	Besides,mediate
R5720	T9952	T9953	det	a,role
R5721	T9953	T9954	nsubjpass	role,proposed
R5722	T9954	T9943	conj	proposed,is
R5723	T9955	T9953	prep	as,role
R5724	T9956	T9955	pobj	antioxidant,as
R5725	T9957	T9954	aux	has,proposed
R5726	T9958	T9954	auxpass	been,proposed
R5727	T9959	T9954	prep	due,proposed
R5728	T9960	T9959	pcomp	to,due
R5729	T9961	T9962	poss	its,ability
R573	T1233	T1231	pcomp	coping,Besides
R5730	T9962	T9959	pobj	ability,due
R5731	T9963	T9964	aux	to,bind
R5732	T9964	T9962	acl	bind,ability
R5733	T9965	T9964	dobj	glutathione,bind
R5734	T9966	T9967	punct	(,35
R5735	T9967	T9954	parataxis	35,proposed
R5736	T9968	T9967	punct	),35
R5737	T9969	T9954	punct	.,proposed
R5738	T9971	T9972	prep	In,renders
R5739	T9973	T9971	pobj	accordance,In
R574	T1234	T1233	prep	with,coping
R5740	T9974	T9973	prep	with,accordance
R5741	T9975	T9974	pobj	this,with
R5742	T9976	T9972	punct	", ",renders
R5743	T9977	T9978	amod	ectopic,expression
R5744	T9978	T9972	nsubj	expression,renders
R5745	T9979	T9978	prep	of,expression
R5746	T9980	T9981	compound	mouse,Sepw1
R5747	T9981	T9979	pobj	Sepw1,of
R5748	T9982	T9972	dobj	cells,renders
R5749	T9983	T9972	oprd	resistant,renders
R575	T1235	T1236	amod	heavy,metal
R5750	T9984	T9983	prep	to,resistant
R5751	T9985	T9986	compound	hydrogen,peroxide
R5752	T9986	T9984	pobj	peroxide,to
R5753	T9987	T9972	punct	", ",renders
R5754	T9988	T9972	cc	and,renders
R5755	T9989	T9990	det	this,resistance
R5756	T9990	T9991	nsubj	resistance,is
R5757	T9991	T9972	conj	is,renders
R5758	T9992	T9991	acomp	dependent,is
R5759	T9993	T9992	prep	on,dependent
R576	T1236	T1237	compound	metal,load
R5760	T9994	T9995	nmod	it,glutathione
R5761	T9995	T9993	pobj	glutathione,on
R5762	T9996	T9995	amod	binding,glutathione
R5763	T9997	T9998	punct	(,36
R5764	T9998	T9991	parataxis	36,is
R5765	T9999	T9998	punct	),36
R5766	T10000	T9991	punct	.,is
R5767	T10002	T10003	advmod	Furthermore,showed
R5768	T10004	T10003	punct	", ",showed
R5769	T10005	T10003	nsubj	Amantana,showed
R577	T1237	T1234	pobj	load,with
R5770	T10006	T10005	nmod	et,Amantana
R5771	T10007	T10005	nmod	al.,Amantana
R5772	T10008	T10009	punct	(,49
R5773	T10009	T10005	parataxis	49,Amantana
R5774	T10010	T10009	punct	),49
R5775	T10011	T10012	mark	that,induced
R5776	T10012	T10003	ccomp	induced,showed
R5777	T10013	T10012	nsubjpass	expression,induced
R5778	T10014	T10013	prep	of,expression
R5779	T10015	T10016	det	a,gene
R578	T1238	T1232	punct	", ",mediate
R5780	T10016	T10014	pobj	gene,of
R5781	T10017	T10016	compound	reporter,gene
R5782	T10018	T10016	acl	fused,gene
R5783	T10019	T10018	prep	to,fused
R5784	T10020	T10021	det	a,fragment
R5785	T10021	T10019	pobj	fragment,to
R5786	T10022	T10021	compound	rat,fragment
R5787	T10023	T10021	compound	Sepw1,fragment
R5788	T10024	T10021	compound	promoter,fragment
R5789	T10025	T10012	aux	can,induced
R579	T1239	T1232	nsubj	MTF,mediate
R5790	T10026	T10012	auxpass	be,induced
R5791	T10027	T10012	prep	in,induced
R5792	T10028	T10029	nmod	rat,cells
R5793	T10029	T10027	pobj	cells,in
R5794	T10030	T10029	amod	glial,cells
R5795	T10031	T10012	agent	by,induced
R5796	T10032	T10031	pobj	copper,by
R5797	T10033	T10032	cc	and,copper
R5798	T10034	T10032	conj	zinc,copper
R5799	T10035	T10034	punct	", ",zinc
R58	T296	T289	punct	),binding
R580	T1240	T1239	punct	-,MTF
R5800	T10036	T10034	cc	but,zinc
R5801	T10037	T10036	neg	not,but
R5802	T10038	T10034	conj	cadmium,zinc
R5803	T10039	T10003	punct	.,showed
R5804	T10041	T10042	det	This,response
R5805	T10042	T10043	nsubj	response,was
R5806	T10044	T10043	acomp	dependent,was
R5807	T10045	T10044	prep	on,dependent
R5808	T10046	T10047	det	an,sequence
R5809	T10047	T10045	pobj	sequence,on
R581	T1241	T1239	nummod	1,MTF
R5810	T10048	T10049	amod	overlapping,inverted
R5811	T10049	T10047	amod	inverted,sequence
R5812	T10050	T10049	punct	-,inverted
R5813	T10051	T10047	compound	MRE,sequence
R5814	T10052	T10047	acl	located,sequence
R5815	T10053	T10052	advmod	proximal,located
R5816	T10054	T10053	prep	to,proximal
R5817	T10055	T10056	det	the,start
R5818	T10056	T10054	pobj	start,to
R5819	T10057	T10056	compound	rat,start
R582	T1242	T1232	aux	can,mediate
R5820	T10058	T10056	compound	Sepw1,start
R5821	T10059	T10056	compound	transcription,start
R5822	T10060	T10061	punct	(,49
R5823	T10061	T10056	parataxis	49,start
R5824	T10062	T10061	punct	),49
R5825	T10063	T10043	punct	", ",was
R5826	T10064	T10065	advmod	even,failed
R5827	T10065	T10043	advcl	failed,was
R5828	T10066	T10065	mark	though,failed
R5829	T10067	T10068	amod	initial,studies
R583	T1243	T1232	advmod	also,mediate
R5830	T10068	T10065	nsubj	studies,failed
R5831	T10069	T10070	aux	to,demonstrate
R5832	T10070	T10065	xcomp	demonstrate,failed
R5833	T10071	T10072	nmod	MTF,binding
R5834	T10072	T10070	dobj	binding,demonstrate
R5835	T10073	T10071	punct	-,MTF
R5836	T10074	T10071	nummod	1,MTF
R5837	T10075	T10072	prep	to,binding
R5838	T10076	T10077	det	that,sequence
R5839	T10077	T10075	pobj	sequence,to
R584	T1244	T1245	det	the,induction
R5840	T10078	T10079	punct	(,50
R5841	T10079	T10070	parataxis	50,demonstrate
R5842	T10080	T10079	punct	),50
R5843	T10081	T10043	punct	.,was
R5844	T10083	T10084	poss	Our,expression
R5845	T10084	T10085	nsubj	expression,suggest
R5846	T10086	T10084	cc	and,expression
R5847	T10087	T10088	npadvmod	DNA,binding
R5848	T10088	T10090	amod	binding,studies
R5849	T10089	T10088	punct	-,binding
R585	T1245	T1232	dobj	induction,mediate
R5850	T10090	T10084	conj	studies,expression
R5851	T10091	T10085	advmod	strongly,suggest
R5852	T10092	T10093	mark	that,is
R5853	T10093	T10085	ccomp	is,suggest
R5854	T10094	T10093	nsubj	MTF,is
R5855	T10095	T10094	punct	-,MTF
R5856	T10096	T10094	nummod	1,MTF
R5857	T10097	T10093	acomp	important,is
R5858	T10098	T10097	prep	for,important
R5859	T10099	T10100	det	the,expression
R586	T1246	T1245	prep	of,induction
R5860	T10100	T10098	pobj	expression,for
R5861	T10101	T10100	amod	basal,expression
R5862	T10102	T10100	prep	of,expression
R5863	T10103	T10104	compound	mouse,Sepw1
R5864	T10104	T10102	pobj	Sepw1,of
R5865	T10105	T10097	prep	by,important
R5866	T10106	T10105	pcomp	binding,by
R5867	T10107	T10106	prep	to,binding
R5868	T10108	T10109	det	the,sequence
R5869	T10109	T10107	pobj	sequence,to
R587	T1247	T1248	compound	Mt,genes
R5870	T10110	T10109	amod	corresponding,sequence
R5871	T10111	T10112	amod	overlapping,inverted
R5872	T10112	T10109	amod	inverted,sequence
R5873	T10113	T10112	punct	-,inverted
R5874	T10114	T10109	compound	MRE,sequence
R5875	T10115	T10085	punct	.,suggest
R5876	T10117	T10118	nsubjpass	Ndrg1,named
R5877	T10119	T10117	punct	", ",Ndrg1
R5878	T10120	T10121	det	another,gene
R5879	T10121	T10117	appos	gene,Ndrg1
R588	T1248	T1246	pobj	genes,of
R5880	T10122	T10121	amod	interesting,gene
R5881	T10123	T10121	nmod	MTF,gene
R5882	T10124	T10123	punct	-,MTF
R5883	T10125	T10123	nummod	1,MTF
R5884	T10126	T10121	compound	target,gene
R5885	T10127	T10118	auxpass	was,named
R5886	T10128	T10129	nmod	N,myc
R5887	T10129	T10131	nmod	myc,gene
R5888	T10130	T10129	punct	-,myc
R5889	T10131	T10118	oprd	gene,named
R589	T1249	T1232	prep	in,mediate
R5890	T10132	T10133	advmod	downstream,regulated
R5891	T10133	T10131	amod	regulated,gene
R5892	T10134	T10131	nummod	1,gene
R5893	T10135	T10118	advcl	following,named
R5894	T10136	T10137	det	the,discovery
R5895	T10137	T10135	dobj	discovery,following
R5896	T10138	T10139	mark	that,represses
R5897	T10139	T10137	acl	represses,discovery
R5898	T10140	T10141	det	the,factor
R5899	T10141	T10139	nsubj	factor,represses
R59	T297	T289	prep	in,binding
R590	T1250	T1249	pobj	response,in
R5900	T10142	T10141	compound	transcription,factor
R5901	T10143	T10144	compound	N,myc
R5902	T10144	T10141	appos	myc,factor
R5903	T10145	T10144	punct	-,myc
R5904	T10146	T10147	det	the,expression
R5905	T10147	T10139	dobj	expression,represses
R5906	T10148	T10147	prep	of,expression
R5907	T10149	T10150	compound	mouse,Ndrg1
R5908	T10150	T10148	pobj	Ndrg1,of
R5909	T10151	T10152	punct	(,51
R591	T1251	T1250	prep	to,response
R5910	T10152	T10118	parataxis	51,named
R5911	T10153	T10152	punct	),51
R5912	T10154	T10118	punct	.,named
R5913	T10156	T10157	nsubjpass	Transcription,induced
R5914	T10158	T10156	prep	of,Transcription
R5915	T10159	T10158	pobj	Ndrg1,of
R5916	T10160	T10159	cc	and,Ndrg1
R5917	T10161	T10160	punct	/,and
R5918	T10162	T10160	cc	or,and
R5919	T10163	T10164	poss	its,ortholog
R592	T1252	T1253	amod	other,situations
R5920	T10164	T10159	conj	ortholog,Ndrg1
R5921	T10165	T10164	amod	human,ortholog
R5922	T10166	T10157	auxpass	is,induced
R5923	T10167	T10157	agent	by,induced
R5924	T10168	T10169	amod	different,conditions
R5925	T10169	T10167	pobj	conditions,by
R5926	T10170	T10169	amod	physiological,conditions
R5927	T10171	T10170	cc	and,physiological
R5928	T10172	T10173	compound	cell,stress
R5929	T10173	T10170	conj	stress,physiological
R593	T1253	T1251	pobj	situations,to
R5930	T10174	T10169	punct	", ",conditions
R5931	T10175	T10176	amod	such,as
R5932	T10176	T10169	prep	as,conditions
R5933	T10177	T10176	pobj	androgens,as
R5934	T10178	T10177	punct	", ",androgens
R5935	T10179	T10180	compound	nickel,compounds
R5936	T10180	T10177	conj	compounds,androgens
R5937	T10181	T10180	punct	", ",compounds
R5938	T10182	T10180	conj	DNA,compounds
R5939	T10183	T10182	nmod	damage,DNA
R594	T1254	T1253	compound	stress,situations
R5940	T10184	T10182	cc	and,DNA
R5941	T10185	T10182	conj	hypoxia,DNA
R5942	T10186	T10187	punct	(,53
R5943	T10187	T10185	parataxis	53,hypoxia
R5944	T10188	T10187	dep	37,53
R5945	T10189	T10190	punct	–,40
R5946	T10190	T10188	prep	40,37
R5947	T10191	T10187	punct	",",53
R5948	T10192	T10187	dep	52,53
R5949	T10193	T10187	punct	",",53
R595	T1255	T1253	punct	", ",situations
R5950	T10194	T10187	punct	),53
R5951	T10195	T10157	punct	.,induced
R5952	T10197	T10198	prep	In,overexpressed
R5953	T10199	T10197	pobj	addition,In
R5954	T10200	T10198	punct	", ",overexpressed
R5955	T10201	T10202	det	the,protein
R5956	T10202	T10198	nsubjpass	protein,overexpressed
R5957	T10203	T10198	auxpass	is,overexpressed
R5958	T10204	T10198	prep	in,overexpressed
R5959	T10205	T10206	amod	human,cancers
R596	T1256	T1257	amod	such,as
R5960	T10206	T10204	pobj	cancers,in
R5961	T10207	T10206	prep	of,cancers
R5962	T10208	T10209	amod	many,tissues
R5963	T10209	T10207	pobj	tissues,of
R5964	T10210	T10209	punct	", ",tissues
R5965	T10211	T10212	amod	such,as
R5966	T10212	T10209	prep	as,tissues
R5967	T10213	T10212	pobj	lung,as
R5968	T10214	T10213	punct	", ",lung
R5969	T10215	T10213	conj	liver,lung
R597	T1257	T1253	prep	as,situations
R5970	T10216	T10215	punct	", ",liver
R5971	T10217	T10215	conj	brain,liver
R5972	T10218	T10217	punct	", ",brain
R5973	T10219	T10217	conj	breast,brain
R5974	T10220	T10219	punct	", ",breast
R5975	T10221	T10219	conj	kidney,breast
R5976	T10222	T10221	cc	and,kidney
R5977	T10223	T10221	conj	skin,kidney
R5978	T10224	T10225	punct	(,40
R5979	T10225	T10198	parataxis	40,overexpressed
R598	T1258	T1259	amod	oxidative,stress
R5980	T10226	T10225	punct	),40
R5981	T10227	T10198	punct	.,overexpressed
R5982	T10229	T10230	mark	Although,studied
R5983	T10230	T10241	advcl	studied,remains
R5984	T10231	T10230	nsubjpass	Ndrg1,studied
R5985	T10232	T10231	cc	and,Ndrg1
R5986	T10233	T10234	advmod	especially,ortholog
R5987	T10234	T10231	conj	ortholog,Ndrg1
R5988	T10235	T10234	poss	its,ortholog
R5989	T10236	T10234	amod	human,ortholog
R599	T1259	T1257	pobj	stress,as
R5990	T10237	T10230	aux	have,studied
R5991	T10238	T10230	auxpass	been,studied
R5992	T10239	T10240	advmod	quite,intensely
R5993	T10240	T10230	advmod	intensely,studied
R5994	T10241	T10245	ccomp	remains,suggests
R5995	T10242	T10241	punct	", ",remains
R5996	T10243	T10244	poss	its,function
R5997	T10244	T10241	nsubj	function,remains
R5998	T10246	T10241	acomp	unclear,remains
R5999	T10247	T10245	punct	;,suggests
R60	T298	T299	det	the,regions
R600	T1260	T1261	punct	(,17
R6000	T10248	T10245	advmod	however,suggests
R6001	T10249	T10245	punct	", ",suggests
R6002	T10250	T10251	det	the,induction
R6003	T10251	T10245	nsubj	induction,suggests
R6004	T10252	T10251	prep	by,induction
R6005	T10253	T10252	pobj	stimuli,by
R6006	T10254	T10253	prep	like,stimuli
R6007	T10255	T10254	pobj	nickel,like
R6008	T10256	T10255	cc	and,nickel
R6009	T10257	T10255	conj	hypoxia,nickel
R601	T1261	T1259	parataxis	17,stress
R6010	T10258	T10259	det	an,involvement
R6011	T10259	T10245	dobj	involvement,suggests
R6012	T10260	T10259	prep	in,involvement
R6013	T10261	T10262	det	the,response
R6014	T10262	T10260	pobj	response,in
R6015	T10263	T10262	compound	cell,response
R6016	T10264	T10262	compound	stress,response
R6017	T10265	T10245	punct	.,suggests
R6018	T10267	T10268	predet	Such,role
R6019	T10268	T10270	nsubjpass	role,endorsed
R602	T1262	T1261	nummod	5,17
R6020	T10269	T10268	det	a,role
R6021	T10271	T10270	auxpass	is,endorsed
R6022	T10272	T10270	advmod	strongly,endorsed
R6023	T10273	T10270	agent	by,endorsed
R6024	T10274	T10275	poss	our,finding
R6025	T10275	T10273	pobj	finding,by
R6026	T10276	T10277	mark	that,induced
R6027	T10277	T10275	advcl	induced,finding
R6028	T10278	T10279	compound	Ndrg1,expression
R6029	T10279	T10277	nsubjpass	expression,induced
R603	T1263	T1261	punct	",",17
R6030	T10280	T10279	compound	gene,expression
R6031	T10281	T10277	auxpass	is,induced
R6032	T10282	T10277	advmod	also,induced
R6033	T10283	T10277	agent	by,induced
R6034	T10284	T10283	pobj	cadmium,by
R6035	T10285	T10277	punct	", ",induced
R6036	T10286	T10277	cc	and,induced
R6037	T10287	T10288	mark	that,plays
R6038	T10288	T10277	conj	plays,induced
R6039	T10289	T10288	nsubj	MTF,plays
R604	T1264	T1261	punct	),17
R6040	T10290	T10289	punct	-,MTF
R6041	T10291	T10289	nummod	1,MTF
R6042	T10292	T10293	det	a,role
R6043	T10293	T10288	dobj	role,plays
R6044	T10294	T10293	amod	crucial,role
R6045	T10295	T10288	prep	in,plays
R6046	T10296	T10297	det	this,induction
R6047	T10297	T10295	pobj	induction,in
R6048	T10298	T10270	punct	.,endorsed
R6049	T10300	T10301	prep	In,indicate
R605	T1265	T1259	cc	and,stress
R6050	T10302	T10303	det	the,case
R6051	T10303	T10300	pobj	case,In
R6052	T10304	T10303	prep	of,case
R6053	T10305	T10304	pobj	Csrp1,of
R6054	T10306	T10301	punct	", ",indicate
R6055	T10307	T10308	nmod	expression,analyses
R6056	T10308	T10309	nmod	analyses,studies
R6057	T10309	T10301	nsubj	studies,indicate
R6058	T10310	T10308	cc	and,analyses
R6059	T10311	T10308	conj	DNA,analyses
R606	T1266	T1259	conj	hypoxia,stress
R6060	T10312	T10311	punct	-,DNA
R6061	T10313	T10311	amod	binding,DNA
R6062	T10314	T10315	mark	that,required
R6063	T10315	T10301	ccomp	required,indicate
R6064	T10316	T10315	nsubjpass	MTF,required
R6065	T10317	T10316	punct	-,MTF
R6066	T10318	T10316	nummod	1,MTF
R6067	T10319	T10315	auxpass	is,required
R6068	T10320	T10315	prep	for,required
R6069	T10321	T10322	compound	cadmium,induction
R607	T1267	T1268	punct	(,18
R6070	T10322	T10320	pobj	induction,for
R6071	T10323	T10315	prep	by,required
R6072	T10324	T10323	pcomp	binding,by
R6073	T10325	T10324	prep	to,binding
R6074	T10326	T10327	det	an,MRE
R6075	T10327	T10325	pobj	MRE,to
R6076	T10328	T10327	advmod	upstream,MRE
R6077	T10329	T10328	prep	of,upstream
R6078	T10330	T10331	det	the,start
R6079	T10331	T10329	pobj	start,of
R608	T1268	T1266	parataxis	18,hypoxia
R6080	T10332	T10331	compound	transcription,start
R6081	T10333	T10301	punct	.,indicate
R6082	T10335	T10336	nsubj	Studies,shown
R6083	T10337	T10335	prep	with,Studies
R6084	T10338	T10339	amod	human,CSRP1
R6085	T10339	T10337	pobj	CSRP1,with
R6086	T10340	T10338	punct	", ",human
R6087	T10341	T10338	conj	avian,human
R6088	T10342	T10341	cc	and,avian
R6089	T10343	T10341	conj	chicken,avian
R609	T1269	T1268	punct	),18
R6090	T10344	T10336	aux	have,shown
R6091	T10345	T10346	mark	that,localized
R6092	T10346	T10336	ccomp	localized,shown
R6093	T10347	T10348	det	this,protein
R6094	T10348	T10346	nsubjpass	protein,localized
R6095	T10349	T10346	auxpass	is,localized
R6096	T10350	T10346	prep	at,localized
R6097	T10351	T10352	compound	adhesion,plaques
R6098	T10352	T10350	pobj	plaques,at
R6099	T10353	T10350	cc	and,at
R61	T299	T297	pobj	regions,in
R610	T1270	T1232	punct	.,mediate
R6100	T10354	T10350	conj	in,at
R6101	T10355	T10354	pobj	association,in
R6102	T10356	T10355	prep	with,association
R6103	T10357	T10358	amod	filamentous,actin
R6104	T10358	T10356	pobj	actin,with
R6105	T10359	T10346	punct	", ",localized
R6106	T10360	T10346	cc	and,localized
R6107	T10361	T10346	conj	interacts,localized
R6108	T10362	T10361	prep	with,interacts
R6109	T10363	T10364	det	the,protein
R611	T1272	T1273	prep	In,required
R6110	T10364	T10362	pobj	protein,with
R6111	T10365	T10364	compound	adhesion,protein
R6112	T10366	T10364	compound	plaque,protein
R6113	T10367	T10364	appos	zyxin,protein
R6114	T10368	T10364	punct	", ",protein
R6115	T10369	T10370	advmod	as,as
R6116	T10370	T10364	cc	as,protein
R6117	T10371	T10370	advmod	well,as
R6118	T10372	T10373	det	the,protein
R6119	T10373	T10364	conj	protein,protein
R612	T1274	T1272	pobj	addition,In
R6120	T10374	T10375	npadvmod	actin,cross-linking
R6121	T10375	T10373	amod	cross-linking,protein
R6122	T10376	T10375	punct	-,cross-linking
R6123	T10377	T10378	compound	alpha,actinin
R6124	T10378	T10373	appos	actinin,protein
R6125	T10379	T10378	punct	-,actinin
R6126	T10380	T10381	punct	(,54
R6127	T10381	T10361	parataxis	54,interacts
R6128	T10382	T10383	punct	–,57
R6129	T10383	T10381	prep	57,54
R613	T1275	T1273	punct	", ",required
R6130	T10384	T10381	punct	),54
R6131	T10385	T10336	punct	.,shown
R6132	T10387	T10388	det	The,ability
R6133	T10388	T10389	nsubj	ability,suggests
R6134	T10390	T10391	aux	to,bind
R6135	T10391	T10388	acl	bind,ability
R6136	T10392	T10393	det	these,partners
R6137	T10393	T10391	dobj	partners,bind
R6138	T10394	T10395	det	a,role
R6139	T10395	T10389	dobj	role,suggests
R614	T1276	T1273	nsubjpass	it,required
R6140	T10396	T10395	prep	in,role
R6141	T10397	T10398	amod	cytoskeletal,organization
R6142	T10398	T10396	pobj	organization,in
R6143	T10399	T10400	punct	(,58
R6144	T10400	T10389	parataxis	58,suggests
R6145	T10401	T10400	punct	),58
R6146	T10402	T10389	punct	.,suggests
R6147	T10404	T10405	nsubj	Exposure,causes
R6148	T10406	T10404	prep	of,Exposure
R6149	T10407	T10408	amod	cultured,cells
R615	T1277	T1273	auxpass	is,required
R6150	T10408	T10406	pobj	cells,of
R6151	T10409	T10404	prep	to,Exposure
R6152	T10410	T10409	pobj	cadmium,to
R6153	T10411	T10412	det	a,decrease
R6154	T10412	T10405	dobj	decrease,causes
R6155	T10413	T10412	prep	in,decrease
R6156	T10414	T10412	punct	", ",decrease
R6157	T10415	T10412	cc	and,decrease
R6158	T10416	T10412	conj	destruction,decrease
R6159	T10417	T10416	prep	of,destruction
R616	T1278	T1273	prep	for,required
R6160	T10418	T10416	punct	", ",destruction
R6161	T10419	T10420	amod	cellular,contact
R6162	T10420	T10421	compound	contact,proteins
R6163	T10421	T10416	conj	proteins,destruction
R6164	T10422	T10421	cc	and,proteins
R6165	T10423	T10424	det	the,cytoskeleton
R6166	T10424	T10421	conj	cytoskeleton,proteins
R6167	T10425	T10424	compound	actin,cytoskeleton
R6168	T10426	T10427	punct	(,59
R6169	T10427	T10405	parataxis	59,causes
R617	T1279	T1280	det	the,metalloregulation
R6170	T10428	T10427	punct	),59
R6171	T10429	T10405	punct	.,causes
R6172	T10431	T10432	prep	In,observed
R6173	T10433	T10434	det	the,cells
R6174	T10434	T10431	pobj	cells,In
R6175	T10435	T10434	amod	proximal,cells
R6176	T10436	T10434	compound	tubule,cells
R6177	T10437	T10434	prep	of,cells
R6178	T10438	T10439	det	the,kidney
R6179	T10439	T10437	pobj	kidney,of
R618	T1280	T1278	pobj	metalloregulation,for
R6180	T10440	T10439	compound	rat,kidney
R6181	T10441	T10432	punct	", ",observed
R6182	T10442	T10443	det	a,loss
R6183	T10443	T10432	nsubjpass	loss,observed
R6184	T10444	T10443	amod	partial,loss
R6185	T10445	T10443	prep	of,loss
R6186	T10446	T10445	pobj	actin,of
R6187	T10447	T10446	cc	and,actin
R6188	T10448	T10449	det	the,protein
R6189	T10449	T10446	conj	protein,actin
R619	T1281	T1280	prep	of,metalloregulation
R6190	T10450	T10451	npadvmod	actin,bundling
R6191	T10451	T10449	amod	bundling,protein
R6192	T10452	T10451	punct	-,bundling
R6193	T10453	T10449	appos	villin,protein
R6194	T10454	T10432	auxpass	is,observed
R6195	T10455	T10432	prep	upon,observed
R6196	T10456	T10457	compound	cadmium,treatment
R6197	T10457	T10455	pobj	treatment,upon
R6198	T10458	T10457	punct	", ",treatment
R6199	T10459	T10460	advmod	as,as
R62	T300	T299	amod	respective,regions
R620	T1282	T1281	pobj	Znt1,of
R6200	T10460	T10457	cc	as,treatment
R6201	T10461	T10460	advmod	well,as
R6202	T10462	T10463	det	the,derangement
R6203	T10463	T10457	conj	derangement,treatment
R6204	T10464	T10463	cc	and,derangement
R6205	T10465	T10463	conj	depolymerization,derangement
R6206	T10466	T10463	prep	of,derangement
R6207	T10467	T10466	pobj	microtubules,of
R6208	T10468	T10469	punct	(,60
R6209	T10469	T10432	parataxis	60,observed
R621	T1283	T1273	punct	", ",required
R6210	T10470	T10469	punct	),60
R6211	T10471	T10432	punct	.,observed
R6212	T10473	T10474	advcl	Assuming,protect
R6213	T10475	T10476	mark	that,is
R6214	T10476	T10473	ccomp	is,Assuming
R6215	T10477	T10476	nsubj	CSRP1,is
R6216	T10478	T10476	acomp	important,is
R6217	T10479	T10478	prep	for,important
R6218	T10480	T10481	det	the,organization
R6219	T10481	T10479	pobj	organization,for
R622	T1284	T1273	advcl	encoding,required
R6220	T10482	T10478	prep	of,important
R6221	T10483	T10484	amod	cytoskeletal,elements
R6222	T10484	T10482	pobj	elements,of
R6223	T10485	T10478	prep	in,important
R6224	T10486	T10487	det	the,mouse
R6225	T10487	T10485	pobj	mouse,in
R6226	T10488	T10474	punct	", ",protect
R6227	T10489	T10490	poss	its,upregulation
R6228	T10490	T10474	nsubj	upregulation,protect
R6229	T10491	T10490	prep	by,upregulation
R623	T1285	T1286	det	the,transporter
R6230	T10492	T10491	pobj	cadmium,by
R6231	T10493	T10474	aux	might,protect
R6232	T10494	T10495	det	the,organism
R6233	T10495	T10474	dobj	organism,protect
R6234	T10496	T10474	prep	from,protect
R6235	T10497	T10496	pobj	damage,from
R6236	T10498	T10497	prep	of,damage
R6237	T10499	T10500	det	the,cytoskeleton
R6238	T10500	T10498	pobj	cytoskeleton,of
R6239	T10501	T10474	punct	.,protect
R624	T1286	T1284	dobj	transporter,encoding
R6240	T10503	T10504	predet	Such,mechanism
R6241	T10504	T10506	nsubj	mechanism,expand
R6242	T10505	T10504	det	a,mechanism
R6243	T10507	T10506	aux	would,expand
R6244	T10508	T10509	det	the,role
R6245	T10509	T10506	dobj	role,expand
R6246	T10510	T10509	prep	of,role
R6247	T10511	T10510	pobj	MTF,of
R6248	T10512	T10511	punct	-,MTF
R6249	T10513	T10511	nummod	1,MTF
R625	T1287	T1286	amod	major,transporter
R6250	T10514	T10509	prep	in,role
R6251	T10515	T10516	compound	stress,response
R6252	T10516	T10514	pobj	response,in
R6253	T10517	T10506	punct	.,expand
R6254	T10519	T10520	poss	Our,studies
R6255	T10520	T10522	nsubj	studies,suggest
R6256	T10521	T10520	compound	expression,studies
R6257	T10523	T10522	advmod	also,suggest
R6258	T10524	T10525	mark	that,represses
R6259	T10525	T10522	ccomp	represses,suggest
R626	T1288	T1289	compound	plasma,membrane
R6260	T10526	T10525	nsubj	MTF,represses
R6261	T10527	T10526	punct	-,MTF
R6262	T10528	T10526	nummod	1,MTF
R6263	T10529	T10530	amod	basal,transcription
R6264	T10530	T10525	dobj	transcription,represses
R6265	T10531	T10530	prep	of,transcription
R6266	T10532	T10531	pobj	Slc39a10,of
R6267	T10533	T10525	punct	", ",represses
R6268	T10534	T10525	prep	in,represses
R6269	T10535	T10534	pobj	contrast,in
R627	T1289	T1290	npadvmod	membrane,localized
R6270	T10536	T10535	prep	to,contrast
R6271	T10537	T10538	poss	its,role
R6272	T10538	T10536	pobj	role,to
R6273	T10539	T10538	prep	as,role
R6274	T10540	T10539	pobj	activator,as
R6275	T10541	T10540	prep	for,activator
R6276	T10542	T10543	det	the,expression
R6277	T10543	T10541	pobj	expression,for
R6278	T10544	T10543	prep	of,expression
R6279	T10545	T10546	amod	other,genes
R628	T1290	T1286	amod	localized,transporter
R6280	T10546	T10544	pobj	genes,of
R6281	T10547	T10546	compound	target,genes
R6282	T10548	T10546	prep	like,genes
R6283	T10549	T10548	pobj	Mt1,like
R6284	T10550	T10549	punct	", ",Mt1
R6285	T10551	T10549	conj	Mt2,Mt1
R6286	T10552	T10551	punct	", ",Mt2
R6287	T10553	T10551	cc	and,Mt2
R6288	T10554	T10551	conj	Znt1,Mt2
R6289	T10555	T10556	punct	(,19
R629	T1291	T1290	punct	-,localized
R6290	T10556	T10525	parataxis	19,represses
R6291	T10557	T10556	nummod	4,19
R6292	T10558	T10556	punct	",",19
R6293	T10559	T10556	punct	),19
R6294	T10560	T10522	punct	.,suggest
R6295	T10562	T10563	nsubj	SLC39A10,is
R6296	T10564	T10563	attr	one,is
R6297	T10565	T10564	prep	of,one
R6298	T10566	T10567	nummod	14,members
R6299	T10567	T10565	pobj	members,of
R63	T301	T302	compound	enhancer,promoter
R630	T1292	T1293	compound	zinc,efflux
R6300	T10568	T10567	compound	mouse,members
R6301	T10569	T10567	compound	SLC39,members
R6302	T10570	T10567	punct	", ",members
R6303	T10571	T10572	dep	which,belong
R6304	T10572	T10567	relcl	belong,members
R6305	T10573	T10572	prep	to,belong
R6306	T10574	T10575	det	the,family
R6307	T10575	T10573	pobj	family,to
R6308	T10576	T10575	compound	ZIP,family
R6309	T10577	T10575	prep	of,family
R631	T1293	T1286	compound	efflux,transporter
R6310	T10578	T10579	compound	metal,ion
R6311	T10579	T10580	compound	ion,transporters
R6312	T10580	T10577	pobj	transporters,of
R6313	T10581	T10582	punct	(,44
R6314	T10582	T10572	parataxis	44,belong
R6315	T10583	T10582	nummod	43,44
R6316	T10584	T10582	punct	",",44
R6317	T10585	T10582	punct	),44
R6318	T10586	T10563	punct	.,is
R6319	T10588	T10589	det	All,members
R632	T1294	T1295	punct	(,19
R6320	T10589	T10590	nsubj	members,increase
R6321	T10591	T10589	prep	of,members
R6322	T10592	T10593	det	the,family
R6323	T10593	T10591	pobj	family,of
R6324	T10594	T10593	compound	ZIP,family
R6325	T10595	T10589	acl	characterized,members
R6326	T10596	T10597	advmod	so,far
R6327	T10597	T10595	advmod	far,characterized
R6328	T10598	T10599	amod	intracellular,concentrations
R6329	T10599	T10590	dobj	concentrations,increase
R633	T1295	T1286	parataxis	19,transporter
R6330	T10600	T10599	amod	cytoplasmic,concentrations
R6331	T10601	T10602	compound	metal,ion
R6332	T10602	T10599	compound	ion,concentrations
R6333	T10603	T10590	prep	by,increase
R6334	T10604	T10603	pcomp	promoting,by
R6335	T10605	T10606	amod	extracellular,transport
R6336	T10606	T10604	dobj	transport,promoting
R6337	T10607	T10605	cc	and,extracellular
R6338	T10608	T10605	conj	vesicular,extracellular
R6339	T10609	T10606	compound	ion,transport
R634	T1296	T1295	punct	),19
R6340	T10610	T10606	prep	into,transport
R6341	T10611	T10612	det	the,cytoplasm
R6342	T10612	T10610	pobj	cytoplasm,into
R6343	T10613	T10590	punct	.,increase
R6344	T10615	T10616	compound	ZIP,proteins
R6345	T10616	T10617	nsubjpass	proteins,reported
R6346	T10618	T10617	aux	have,reported
R6347	T10619	T10617	auxpass	been,reported
R6348	T10620	T10621	aux	to,be
R6349	T10621	T10617	xcomp	be,reported
R635	T1297	T1286	punct	", ",transporter
R6350	T10622	T10621	attr	transporters,be
R6351	T10623	T10622	prep	of,transporters
R6352	T10624	T10623	pobj	zinc,of
R6353	T10625	T10624	punct	", ",zinc
R6354	T10626	T10624	conj	iron,zinc
R6355	T10627	T10626	punct	", ",iron
R6356	T10628	T10626	conj	manganese,iron
R6357	T10629	T10628	cc	and,manganese
R6358	T10630	T10629	punct	/,and
R6359	T10631	T10629	cc	or,and
R636	T1298	T1299	det	the,induction
R6360	T10632	T10628	conj	cadmium,manganese
R6361	T10633	T10634	punct	(,44
R6362	T10634	T10621	parataxis	44,be
R6363	T10635	T10634	punct	",",44
R6364	T10636	T10634	appos	61,44
R6365	T10637	T10638	punct	–,63
R6366	T10638	T10636	prep	63,61
R6367	T10639	T10634	punct	),44
R6368	T10640	T10617	punct	.,reported
R6369	T10642	T10643	mark	Although,is
R637	T1299	T1286	appos	induction,transporter
R6370	T10643	T10645	advcl	is,referred
R6371	T10644	T10643	nsubj	SLC39A10,is
R6372	T10646	T10647	advmod	largely,uncharacterized
R6373	T10647	T10643	acomp	uncharacterized,is
R6374	T10648	T10649	punct	(,44
R6375	T10649	T10643	parataxis	44,is
R6376	T10650	T10649	punct	),44
R6377	T10651	T10645	punct	", ",referred
R6378	T10652	T10645	nsubjpass	it,referred
R6379	T10653	T10645	auxpass	is,referred
R638	T1300	T1301	amod	hypoxic,anoxic
R6380	T10654	T10645	prep	to,referred
R6381	T10655	T10645	prep	in,referred
R6382	T10656	T10657	amod	several,databases
R6383	T10657	T10655	pobj	databases,in
R6384	T10658	T10645	prep	as,referred
R6385	T10659	T10660	amod	putative,transporter
R6386	T10660	T10658	pobj	transporter,as
R6387	T10661	T10660	compound	zinc,transporter
R6388	T10662	T10645	punct	.,referred
R6389	T10664	T10665	nsubjpass	It,shown
R639	T1301	T1299	amod	anoxic,induction
R6390	T10666	T10665	aux	has,shown
R6391	T10667	T10665	auxpass	been,shown
R6392	T10668	T10665	advmod	previously,shown
R6393	T10669	T10670	mark	that,is
R6394	T10670	T10665	ccomp	is,shown
R6395	T10671	T10670	nsubj	MTF,is
R6396	T10672	T10671	punct	-,MTF
R6397	T10673	T10671	nummod	1,MTF
R6398	T10674	T10670	acomp	important,is
R6399	T10675	T10674	prep	for,important
R64	T302	T299	compound	promoter,regions
R640	T1302	T1301	punct	/,anoxic
R6400	T10676	T10677	preconj	both,expression
R6401	T10677	T10675	pobj	expression,for
R6402	T10678	T10677	amod	basal,expression
R6403	T10679	T10677	cc	and,expression
R6404	T10680	T10681	compound	metal,induction
R6405	T10681	T10677	conj	induction,expression
R6406	T10682	T10677	prep	of,expression
R6407	T10683	T10684	det	the,gene
R6408	T10684	T10682	pobj	gene,of
R6409	T10685	T10684	compound	mouse,gene
R641	T1303	T1299	prep	of,induction
R6410	T10686	T10684	compound	Znt1,gene
R6411	T10687	T10688	punct	(,19
R6412	T10688	T10670	parataxis	19,is
R6413	T10689	T10688	punct	),19
R6414	T10690	T10665	punct	.,shown
R6415	T10692	T10693	compound	ZnT,proteins
R6416	T10693	T10694	nsubj	proteins,represent
R6417	T10695	T10696	det	a,family
R6418	T10696	T10694	dobj	family,represent
R6419	T10697	T10696	amod	different,family
R642	T1304	T1305	det	the,gene
R6420	T10698	T10696	prep	of,family
R6421	T10699	T10698	pobj	transporters,of
R6422	T10700	T10701	dep	that,reduce
R6423	T10701	T10696	relcl	reduce,family
R6424	T10702	T10703	amod	intracellular,zinc
R6425	T10703	T10701	dobj	zinc,reduce
R6426	T10704	T10703	amod	cytoplasmic,zinc
R6427	T10705	T10701	prep	by,reduce
R6428	T10706	T10705	pcomp	promoting,by
R6429	T10707	T10708	compound	zinc,efflux
R643	T1305	T1303	pobj	gene,of
R6430	T10708	T10706	dobj	efflux,promoting
R6431	T10709	T10708	prep	from,efflux
R6432	T10710	T10709	pobj	cells,from
R6433	T10711	T10709	cc	or,from
R6434	T10712	T10709	conj	into,from
R6435	T10713	T10714	amod	intracellular,vesicles
R6436	T10714	T10712	pobj	vesicles,into
R6437	T10715	T10694	punct	.,represent
R6438	T10717	T10718	advmod	Thus,have
R6439	T10719	T10718	punct	", ",have
R644	T1306	T1305	prep	for,gene
R6440	T10720	T10718	nsubj	members,have
R6441	T10721	T10720	prep	of,members
R6442	T10722	T10723	det	the,family
R6443	T10723	T10721	pobj	family,of
R6444	T10724	T10723	nmod	ZnT,family
R6445	T10725	T10724	cc	and,ZnT
R6446	T10726	T10724	conj	ZIP,ZnT
R6447	T10727	T10720	prep	with,members
R6448	T10728	T10727	pobj	zinc,with
R6449	T10729	T10728	prep	as,zinc
R645	T1307	T1308	amod	placental,factor
R6450	T10730	T10731	amod	predominant,substrate
R6451	T10731	T10729	pobj	substrate,as
R6452	T10732	T10733	amod	opposite,roles
R6453	T10733	T10718	dobj	roles,have
R6454	T10734	T10733	prep	in,roles
R6455	T10735	T10736	amod	cellular,homeostasis
R6456	T10736	T10734	pobj	homeostasis,in
R6457	T10737	T10736	compound	zinc,homeostasis
R6458	T10738	T10739	punct	(,43
R6459	T10739	T10718	parataxis	43,have
R646	T1308	T1306	pobj	factor,for
R6460	T10740	T10739	punct	),43
R6461	T10741	T10718	punct	.,have
R6462	T10743	T10744	advcl	Assuming,control
R6463	T10745	T10746	mark	that,is
R6464	T10746	T10743	ccomp	is,Assuming
R6465	T10747	T10746	nsubj	SLC39A10,is
R6466	T10748	T10746	advmod	indeed,is
R6467	T10749	T10750	det	a,transporter
R6468	T10750	T10746	attr	transporter,is
R6469	T10751	T10750	compound	zinc,transporter
R647	T1309	T1308	compound	growth,factor
R6470	T10752	T10744	punct	", ",control
R6471	T10753	T10744	nsubj	MTF,control
R6472	T10754	T10753	punct	-,MTF
R6473	T10755	T10753	nummod	1,MTF
R6474	T10756	T10744	aux	would,control
R6475	T10757	T10744	dobj	expression,control
R6476	T10758	T10757	prep	of,expression
R6477	T10759	T10760	nummod	two,transporters
R6478	T10760	T10758	pobj	transporters,of
R6479	T10761	T10760	compound	zinc,transporters
R648	T1310	T1305	punct	(,gene
R6480	T10762	T10760	prep	with,transporters
R6481	T10763	T10764	amod	antagonistic,functions
R6482	T10764	T10762	pobj	functions,with
R6483	T10765	T10760	punct	", ",transporters
R6484	T10766	T10767	advmod	namely,Znt1
R6485	T10767	T10760	appos	Znt1,transporters
R6486	T10768	T10767	punct	", ",Znt1
R6487	T10769	T10767	cc	and,Znt1
R6488	T10770	T10767	conj	Slc39a10,Znt1
R6489	T10771	T10744	punct	.,control
R649	T1311	T1305	appos	Plgf,gene
R6490	T10773	T10774	amod	Specific,binding
R6491	T10774	T10775	nsubjpass	binding,observed
R6492	T10776	T10774	prep	of,binding
R6493	T10777	T10776	pobj	MTF,of
R6494	T10778	T10777	punct	-,MTF
R6495	T10779	T10777	nummod	1,MTF
R6496	T10780	T10775	auxpass	was,observed
R6497	T10781	T10775	prep	for,observed
R6498	T10782	T10783	det	an,MRE
R6499	T10783	T10781	pobj	MRE,for
R65	T303	T302	punct	/,promoter
R650	T1312	T1286	punct	),transporter
R6500	T10784	T10783	acl	located,MRE
R6501	T10785	T10786	advmod	just,downstream
R6502	T10786	T10784	advmod	downstream,located
R6503	T10787	T10786	prep	of,downstream
R6504	T10788	T10789	det	the,start
R6505	T10789	T10787	pobj	start,of
R6506	T10790	T10789	compound	Slc39a10,start
R6507	T10791	T10789	compound	transcription,start
R6508	T10792	T10775	punct	.,observed
R6509	T10794	T10795	prep	In,interfere
R651	T1313	T1286	punct	", ",transporter
R6510	T10796	T10797	det	a,model
R6511	T10797	T10794	pobj	model,In
R6512	T10798	T10797	amod	simple,model
R6513	T10799	T10795	punct	", ",interfere
R6514	T10800	T10801	predet	such,binding
R6515	T10801	T10795	nsubj	binding,interfere
R6516	T10802	T10801	det	a,binding
R6517	T10803	T10795	aux	could,interfere
R6518	T10804	T10795	prep	with,interfere
R6519	T10805	T10806	det	the,accessibility
R652	T1314	T1315	det	an,protein
R6520	T10806	T10804	pobj	accessibility,with
R6521	T10807	T10806	prep	of,accessibility
R6522	T10808	T10809	det	the,site
R6523	T10809	T10807	pobj	site,of
R6524	T10810	T10809	amod	transcriptional,site
R6525	T10811	T10809	compound	start,site
R6526	T10812	T10809	prep	for,site
R6527	T10813	T10814	compound	RNA,polymerase
R6528	T10814	T10812	pobj	polymerase,for
R6529	T10815	T10814	nummod	II,polymerase
R653	T1315	T1286	appos	protein,transporter
R6530	T10816	T10814	cc	and,polymerase
R6531	T10817	T10816	punct	/,and
R6532	T10818	T10816	cc	or,and
R6533	T10819	T10820	amod	general,factors
R6534	T10820	T10814	conj	factors,polymerase
R6535	T10821	T10820	compound	transcription,factors
R6536	T10822	T10795	punct	", ",interfere
R6537	T10823	T10824	advmod	thus,preventing
R6538	T10824	T10795	advcl	preventing,interfere
R6539	T10825	T10826	compound	transcription,initiation
R654	T1316	T1315	amod	angiogenic,protein
R6540	T10826	T10824	dobj	initiation,preventing
R6541	T10827	T10826	prep	of,initiation
R6542	T10828	T10829	det	the,gene
R6543	T10829	T10827	pobj	gene,of
R6544	T10830	T10795	punct	.,interfere
R6545	T10832	T10833	advmod	Indeed,described
R6546	T10834	T10833	punct	", ",described
R6547	T10835	T10836	predet	such,mechanism
R6548	T10836	T10833	nsubjpass	mechanism,described
R6549	T10837	T10836	det	a,mechanism
R655	T1317	T1315	prep	of,protein
R6550	T10838	T10833	aux	has,described
R6551	T10839	T10833	auxpass	been,described
R6552	T10840	T10833	prep	in,described
R6553	T10841	T10840	pobj	yeast,in
R6554	T10842	T10833	prep	for,described
R6555	T10843	T10844	det	the,protein
R6556	T10844	T10842	pobj	protein,for
R6557	T10845	T10846	npadvmod	zinc,responsive
R6558	T10846	T10844	amod	responsive,protein
R6559	T10847	T10846	punct	-,responsive
R656	T1318	T1319	det	the,family
R6560	T10848	T10844	compound	activator,protein
R6561	T10849	T10844	nummod	1,protein
R6562	T10850	T10844	punct	(,protein
R6563	T10851	T10844	appos	Zap1,protein
R6564	T10852	T10844	punct	),protein
R6565	T10853	T10844	cc	and,protein
R6566	T10854	T10855	poss	its,gene
R6567	T10855	T10844	conj	gene,protein
R6568	T10856	T10855	compound	target,gene
R6569	T10857	T10855	punct	", ",gene
R657	T1319	T1317	pobj	family,of
R6570	T10858	T10859	nmod	zinc,transporter
R6571	T10859	T10855	appos	transporter,gene
R6572	T10860	T10859	punct	-,transporter
R6573	T10861	T10859	amod	regulated,transporter
R6574	T10862	T10859	nummod	2,transporter
R6575	T10863	T10859	punct	(,transporter
R6576	T10864	T10859	appos	ZRT2,transporter
R6577	T10865	T10833	punct	),described
R6578	T10866	T10867	punct	(,64
R6579	T10867	T10833	parataxis	64,described
R658	T1320	T1321	amod	vascular,factor
R6580	T10868	T10867	punct	),64
R6581	T10869	T10833	punct	.,described
R6582	T10871	T10872	advmod	However,be
R6583	T10873	T10872	punct	", ",be
R6584	T10874	T10875	det	the,inhibition
R6585	T10875	T10872	nsubj	inhibition,be
R6586	T10876	T10875	prep	of,inhibition
R6587	T10877	T10878	compound	Slc39a10,expression
R6588	T10878	T10876	pobj	expression,of
R6589	T10879	T10875	prep	by,inhibition
R659	T1321	T1319	nmod	factor,family
R6590	T10880	T10879	pobj	MTF,by
R6591	T10881	T10880	punct	-,MTF
R6592	T10882	T10880	nummod	1,MTF
R6593	T10883	T10872	aux	may,be
R6594	T10884	T10872	advmod	well,be
R6595	T10885	T10886	advmod	more,complex
R6596	T10886	T10872	acomp	complex,be
R6597	T10887	T10886	prep	than,complex
R6598	T10888	T10889	det	a,competition
R6599	T10889	T10887	pobj	competition,than
R66	T304	T264	punct	.,regulates
R660	T1322	T1321	amod	endothelial,factor
R6600	T10890	T10889	prep	for,competition
R6601	T10891	T10892	compound	promoter,binding
R6602	T10892	T10890	pobj	binding,for
R6603	T10893	T10872	punct	.,be
R6604	T10895	T10896	advcl	Independent,leads
R6605	T10897	T10895	prep	of,Independent
R6606	T10898	T10897	pobj	MTF,of
R6607	T10899	T10898	punct	-,MTF
R6608	T10900	T10898	nummod	1,MTF
R6609	T10901	T10896	punct	", ",leads
R661	T1323	T1321	nmod	growth,factor
R6610	T10902	T10903	compound	cadmium,treatment
R6611	T10903	T10896	nsubj	treatment,leads
R6612	T10904	T10896	advmod	also,leads
R6613	T10905	T10896	prep	to,leads
R6614	T10906	T10905	pobj	downregulation,to
R6615	T10907	T10906	prep	of,downregulation
R6616	T10908	T10909	compound	Slc39a10,transcripts
R6617	T10909	T10907	pobj	transcripts,of
R6618	T10910	T10896	punct	", ",leads
R6619	T10911	T10896	advcl	suggesting,leads
R662	T1324	T1321	punct	(,factor
R6620	T10912	T10913	mark	that,mediating
R6621	T10913	T10911	ccomp	mediating,suggesting
R6622	T10914	T10915	det	some,factor
R6623	T10915	T10913	nsubj	factor,mediating
R6624	T10916	T10915	amod	other,factor
R6625	T10917	T10913	aux	is,mediating
R6626	T10918	T10919	det	this,response
R6627	T10919	T10913	dobj	response,mediating
R6628	T10920	T10896	punct	.,leads
R6629	T10922	T10923	det	A,search
R663	T1325	T1321	appos	VEGF,factor
R6630	T10923	T10927	nsubj	search,revealed
R6631	T10924	T10923	amod	previous,search
R6632	T10925	T10926	compound	target,gene
R6633	T10926	T10923	compound	gene,search
R6634	T10928	T10923	prep	for,search
R6635	T10929	T10928	pobj	MTF,for
R6636	T10930	T10929	punct	-,MTF
R6637	T10931	T10929	nummod	1,MTF
R6638	T10932	T10923	prep	with,search
R6639	T10933	T10934	compound	mouse,embryos
R664	T1326	T1319	punct	),family
R6640	T10934	T10932	pobj	embryos,with
R6641	T10935	T10934	prep	of,embryos
R6642	T10936	T10937	amod	conventional,phenotype
R6643	T10937	T10935	pobj	phenotype,of
R6644	T10938	T10937	compound	Mtf1,phenotype
R6645	T10939	T10937	compound	knockout,phenotype
R6646	T10940	T10927	punct	", ",revealed
R6647	T10941	T10927	prep	besides,revealed
R6648	T10942	T10943	compound	metallothionein,genes
R6649	T10943	T10941	pobj	genes,besides
R665	T1327	T1328	punct	(,20
R6650	T10944	T10927	punct	", ",revealed
R6651	T10945	T10946	det	the,fetoprotein
R6652	T10946	T10927	dobj	fetoprotein,revealed
R6653	T10947	T10946	amod	multifunctional,fetoprotein
R6654	T10948	T10946	compound	alpha,fetoprotein
R6655	T10949	T10946	punct	-,fetoprotein
R6656	T10950	T10946	punct	(,fetoprotein
R6657	T10951	T10946	appos	Afp,fetoprotein
R6658	T10952	T10946	punct	),fetoprotein
R6659	T10953	T10946	cc	and,fetoprotein
R666	T1328	T1319	parataxis	20,family
R6660	T10954	T10955	det	the,factor
R6661	T10955	T10946	conj	factor,fetoprotein
R6662	T10956	T10957	npadvmod	liver,enriched
R6663	T10957	T10955	amod	enriched,factor
R6664	T10958	T10957	punct	-,enriched
R6665	T10959	T10955	compound	transcription,factor
R6666	T10960	T10961	compound	CCAAT,protein
R6667	T10961	T10955	appos	protein,factor
R6668	T10962	T10961	punct	/,protein
R6669	T10963	T10961	compound	enhancer,protein
R667	T1329	T1328	punct	),20
R6670	T10964	T10961	compound	binding,protein
R6671	T10965	T10961	punct	alpha,protein
R6672	T10966	T10961	punct	(,protein
R6673	T10967	T10961	appos	Cebpa,protein
R6674	T10968	T10927	punct	),revealed
R6675	T11052	T11051	cc	and,Mtf1loxP
R6676	T10969	T10927	prep	as,revealed
R6677	T11053	T11054	compound	Mtf1Mx,cre
R6678	T10970	T10971	amod	prime,candidates
R6679	T11054	T11051	conj	cre,Mtf1loxP
R668	T1330	T1273	punct	", ",required
R6680	T11055	T11054	punct	-,cre
R6681	T10971	T10969	pobj	candidates,as
R6682	T11056	T11057	punct	(,shown
R6683	T11057	T11041	parataxis	shown,revealed
R6684	T11058	T11057	nsubj	data,shown
R6685	T10972	T10973	punct	(,65
R6686	T11059	T11057	neg	not,shown
R6687	T11060	T11057	punct	),shown
R6688	T10973	T10927	parataxis	65,revealed
R6689	T11061	T11041	punct	.,revealed
R669	T1331	T1273	cc	and,required
R6690	T10974	T10973	punct	),65
R6691	T11063	T11064	advmod	Therefore,affect
R6692	T11065	T11064	punct	", ",affect
R6693	T10975	T10927	punct	.,revealed
R6694	T11066	T11064	nsubj	MTF,affect
R6695	T11067	T11066	punct	-,MTF
R6696	T11068	T11066	nummod	1,MTF
R6697	T10977	T10978	prep	After,repressed
R6698	T11069	T11064	aux	may,affect
R6699	T11070	T11071	compound	Cebpa,expression
R67	T306	T307	advmod	Furthermore,serves
R670	T1332	T1333	aux	has,invoked
R6700	T11071	T11064	dobj	expression,affect
R6701	T11072	T11073	advmod	only,during
R6702	T10979	T10980	det	an,onset
R6703	T11073	T11064	prep	during,affect
R6704	T11074	T11075	amod	embryonal,development
R6705	T11075	T11073	pobj	development,during
R6706	T11076	T11064	punct	", ",affect
R6707	T10980	T10977	pobj	onset,After
R6708	T11077	T11078	advmod	perhaps,in
R6709	T11078	T11064	prep	in,affect
R671	T1333	T1273	conj	invoked,required
R6710	T10981	T10980	amod	early,onset
R6711	T11079	T11078	pobj	combination,in
R6712	T11080	T11079	prep	with,combination
R6713	T11081	T11082	advmod	as,yet
R6714	T10982	T10978	prep	during,repressed
R6715	T10983	T10982	pobj	hepatogenesis,during
R6716	T10984	T10978	punct	", ",repressed
R6717	T11082	T11083	advmod	yet,unidentified
R6718	T11083	T11084	amod	unidentified,factors
R6719	T10985	T10986	compound	Afp,expression
R672	T1334	T1333	advmod	recently,invoked
R6720	T11084	T11080	pobj	factors,with
R6721	T11085	T11064	punct	.,affect
R6722	T10986	T10978	nsubjpass	expression,repressed
R6723	T11087	T11088	det	The,study
R6724	T11088	T11090	nsubj	study,confirms
R6725	T11089	T11088	amod	present,study
R6726	T10987	T10978	auxpass	is,repressed
R6727	T11091	T11090	cc	and,confirms
R6728	T11092	T11090	conj	extends,confirms
R6729	T10988	T10978	advmod	postnatally,repressed
R673	T1335	T1333	auxpass	been,invoked
R6730	T11093	T11094	det	the,role
R6731	T11094	T11092	dobj	role,extends
R6732	T11095	T11094	prep	of,role
R6733	T10989	T10978	cc	and,repressed
R6734	T11096	T11095	pobj	MTF,of
R6735	T11097	T11096	punct	-,MTF
R6736	T11098	T11096	nummod	1,MTF
R6737	T10990	T10978	conj	replaced,repressed
R6738	T11099	T11094	prep	as,role
R6739	T11100	T11101	det	an,regulator
R674	T1336	T1333	prep	in,invoked
R6740	T10991	T10990	agent	by,replaced
R6741	T11101	T11099	pobj	regulator,as
R6742	T11102	T11101	amod	important,regulator
R6743	T11103	T11104	compound	stress,response
R6744	T10992	T10991	pobj	albumin,by
R6745	T11104	T11101	compound	response,regulator
R6746	T11105	T11090	punct	.,confirms
R6747	T11107	T11108	nsubj	We,identified
R6748	T11108	T11110	ccomp	identified,required
R6749	T11109	T11108	aux	have,identified
R675	T1337	T1338	compound	tumor,development
R6750	T10993	T10994	punct	(,66
R6751	T11111	T11108	cc	and,identified
R6752	T11112	T11113	advmod	preliminarily,characterized
R6753	T10994	T10990	parataxis	66,replaced
R6754	T11113	T11108	conj	characterized,identified
R6755	T11114	T11115	nummod	four,genes
R6756	T11115	T11113	dobj	genes,characterized
R6757	T10995	T10994	punct	),66
R6758	T11116	T11115	compound	target,genes
R6759	T11117	T11115	prep	of,genes
R676	T1338	T1336	pobj	development,in
R6760	T10996	T10978	punct	.,repressed
R6761	T11118	T11117	pobj	MTF,of
R6762	T11119	T11118	punct	-,MTF
R6763	T11120	T11118	nummod	1,MTF
R6764	T10998	T10999	advmod	Thus,were
R6765	T11121	T11115	prep	in,genes
R6766	T11122	T11123	det	the,liver
R6767	T11123	T11121	pobj	liver,in
R6768	T11124	T11123	amod	adult,liver
R6769	T11000	T10999	punct	", ",were
R677	T1339	T1340	punct	(,22
R6770	T11125	T11123	compound	mouse,liver
R6771	T11126	T11110	punct	: ,required
R6772	T11001	T11002	poss	our,mice
R6773	T11127	T11110	prep	in,required
R6774	T11128	T11129	det	the,case
R6775	T11129	T11127	pobj	case,in
R6776	T11130	T11129	prep	of,case
R6777	T11002	T10999	nsubj	mice,were
R6778	T11131	T11130	pobj	Sepw1,of
R6779	T11132	T11110	punct	", ",required
R678	T1340	T1333	parataxis	22,invoked
R6780	T11133	T11110	nsubjpass	MTF,required
R6781	T11003	T11002	amod	adult,mice
R6782	T11134	T11133	punct	-,MTF
R6783	T11135	T11133	nummod	1,MTF
R6784	T11004	T11005	compound	Mtf1Mx,cre
R6785	T11136	T11110	auxpass	is,required
R6786	T11137	T11138	aux	to,maintain
R6787	T11138	T11110	advcl	maintain,required
R6788	T11005	T11002	compound	cre,mice
R6789	T11139	T11140	amod	basal,expression
R679	T1341	T1340	nummod	21,22
R6790	T11140	T11138	dobj	expression,maintain
R6791	T11141	T11110	punct	", ",required
R6792	T11006	T11005	punct	-,cre
R6793	T11142	T11110	advcl	supporting,required
R6794	T11143	T11144	det	a,role
R6795	T11144	T11142	dobj	role,supporting
R6796	T11007	T11002	acl	lacking,mice
R6797	T11145	T11144	prep	of,role
R6798	T11146	T11147	compound	mouse,MTF
R6799	T11147	T11145	pobj	MTF,of
R68	T308	T307	punct	", ",serves
R680	T1342	T1340	punct	",",22
R6800	T11148	T11147	punct	-,MTF
R6801	T11149	T11147	nummod	1,MTF
R6802	T11150	T11144	prep	in,role
R6803	T11008	T11007	dobj	MTF,lacking
R6804	T11151	T11152	amod	oxidative,response
R6805	T11009	T11008	punct	-,MTF
R6806	T11152	T11150	pobj	response,in
R6807	T11153	T11152	compound	stress,response
R6808	T11010	T11008	nummod	1,MTF
R6809	T11154	T11110	punct	.,required
R681	T1343	T1340	punct	),22
R6810	T11156	T11157	prep	In,contributes
R6811	T11011	T10999	neg	not,were
R6812	T11012	T10999	acomp	suitable,were
R6813	T11013	T11014	aux	to,analyze
R6814	T11014	T10999	advcl	analyze,were
R6815	T11015	T11016	compound	Afp,expression
R6816	T11158	T11156	pobj	addition,In
R6817	T11159	T11157	punct	", ",contributes
R6818	T11160	T11157	nsubj	MTF,contributes
R6819	T11016	T11014	dobj	expression,analyze
R682	T1344	T1273	punct	.,required
R6820	T11161	T11160	punct	-,MTF
R6821	T11162	T11160	nummod	1,MTF
R6822	T11017	T10999	punct	.,were
R6823	T11163	T11157	prep	to,contributes
R6824	T11164	T11165	det	the,expression
R6825	T11165	T11163	pobj	expression,to
R6826	T11019	T11020	nsubjpass	Cebpa,expressed
R6827	T11166	T11167	npadvmod	cadmium,induced
R6828	T11167	T11165	amod	induced,expression
R6829	T11168	T11167	punct	-,induced
R683	T1346	T1347	advmod	Furthermore,has
R6830	T11021	T11020	auxpass	is,expressed
R6831	T11169	T11165	prep	of,expression
R6832	T11170	T11169	pobj	Ndrg1,of
R6833	T11171	T11170	cc	and,Ndrg1
R6834	T11172	T11170	conj	Csrp1,Ndrg1
R6835	T11022	T11020	prep	in,expressed
R6836	T11173	T11157	punct	.,contributes
R6837	T11175	T11176	advmod	Furthermore,helps
R6838	T11023	T11024	det	the,liver
R6839	T11177	T11176	punct	", ",helps
R684	T1347	T1352	ccomp	has,results
R6840	T11024	T11022	pobj	liver,in
R6841	T11178	T11176	nsubj	MTF,helps
R6842	T11179	T11178	punct	-,MTF
R6843	T11180	T11178	nummod	1,MTF
R6844	T11025	T11024	amod	adult,liver
R6845	T11181	T11182	aux	to,repress
R6846	T11182	T11176	xcomp	repress,helps
R6847	T11183	T11184	det	the,expression
R6848	T11026	T11027	advmod	as,as
R6849	T11184	T11182	dobj	expression,repress
R685	T1348	T1347	punct	", ",has
R6850	T11185	T11184	amod	basal,expression
R6851	T11186	T11184	prep	of,expression
R6852	T11187	T11186	pobj	Slc39a10,of
R6853	T11188	T11182	punct	", ",repress
R6854	T11189	T11182	prep	in,repress
R6855	T11027	T11022	cc	as,in
R6856	T11190	T11189	pobj	contrast,in
R6857	T11191	T11190	prep	to,contrast
R6858	T11192	T11193	poss	its,role
R6859	T11193	T11191	pobj	role,to
R686	T1349	T1347	nsubj	MTF,has
R6860	T11028	T11027	advmod	well,as
R6861	T11194	T11193	prep	as,role
R6862	T11195	T11196	amod	transcriptional,activator
R6863	T11029	T11022	conj	in,in
R6864	T11196	T11194	pobj	activator,as
R6865	T11197	T11196	prep	for,activator
R6866	T11198	T11197	pobj	genes,for
R6867	T11030	T11031	amod	other,tissues
R6868	T11199	T11198	prep	like,genes
R6869	T11200	T11199	pobj	Mt1,like
R687	T1350	T1349	punct	-,MTF
R6870	T11201	T11200	punct	", ",Mt1
R6871	T11031	T11029	pobj	tissues,in
R6872	T11202	T11200	conj	Mt2,Mt1
R6873	T11203	T11202	cc	or,Mt2
R6874	T11204	T11202	conj	Znt1,Mt2
R6875	T11032	T11033	punct	(,67
R6876	T11205	T11176	punct	.,helps
R6877	T11033	T11020	parataxis	67,expressed
R6878	T11207	T11208	advmod	Thus,serves
R6879	T11209	T11210	det	the,factor
R688	T1351	T1349	nummod	1,MTF
R6880	T11034	T11033	punct	),67
R6881	T11210	T11208	nsubj	factor,serves
R6882	T11211	T11210	amod	same,factor
R6883	T11035	T11020	punct	", ",expressed
R6884	T11212	T11210	compound	transcription,factor
R6885	T11213	T11208	advmod	apparently,serves
R6886	T11214	T11208	prep	as,serves
R6887	T11215	T11216	det	an,activator
R6888	T11036	T11020	cc	but,expressed
R6889	T11216	T11214	pobj	activator,as
R689	T1353	T1354	det	an,function
R6890	T11217	T11216	cc	or,activator
R6891	T11218	T11216	conj	repressor,activator
R6892	T11037	T11038	det	the,data
R6893	T11219	T11208	punct	", ",serves
R6894	T11220	T11208	prep	depending,serves
R6895	T11221	T11220	prep	on,depending
R6896	T11038	T11041	nsubj	data,revealed
R6897	T11222	T11223	det	the,gene
R6898	T11223	T11221	pobj	gene,on
R6899	T11224	T11223	compound	target,gene
R69	T309	T307	nsubj	it,serves
R690	T1354	T1347	dobj	function,has
R6900	T11039	T11038	amod	present,data
R6901	T11225	T11208	punct	.,serves
R6902	T11040	T11038	compound	microarray,data
R6903	T11227	T11228	det	The,comparison
R6904	T11228	T11229	nsubj	comparison,revealed
R6905	T11230	T11228	prep	of,comparison
R6906	T11231	T11232	compound	liver,gene
R6907	T11232	T11233	compound	gene,expression
R6908	T11041	T11020	conj	revealed,expressed
R6909	T11233	T11230	pobj	expression,of
R691	T1355	T1354	amod	essential,function
R6910	T11234	T11233	prep	of,expression
R6911	T11235	T11236	npadvmod	cadmium,treated
R6912	T11236	T11241	amod	treated,mice
R6913	T11042	T11043	det	no,differences
R6914	T11237	T11235	punct	-,cadmium
R6915	T11238	T11235	cc	and,cadmium
R6916	T11043	T11041	dobj	differences,revealed
R6917	T11239	T11235	conj	mock,cadmium
R6918	T11240	T11236	punct	-,treated
R6919	T11044	T11043	amod	significant,differences
R692	T1356	T1347	prep	during,has
R6920	T11045	T11043	compound	expression,differences
R6921	T11241	T11234	pobj	mice,of
R6922	T11046	T11041	prep	in,revealed
R6923	T11242	T11229	advmod	also,revealed
R6924	T11243	T11244	det	a,number
R6925	T11244	T11229	dobj	number,revealed
R6926	T11047	T11046	pobj	livers,in
R6927	T11245	T11244	prep	of,number
R6928	T11246	T11245	pobj	genes,of
R6929	T11048	T11047	prep	from,livers
R693	T1357	T1356	pobj	embryogenesis,during
R6930	T11247	T11248	dep	that,were
R6931	T11248	T11246	relcl	were,genes
R6932	T11249	T11248	acomp	responsive,were
R6933	T11049	T11050	amod	adult,mice
R6934	T11250	T11249	prep	to,responsive
R6935	T11251	T11252	compound	cadmium,exposure
R6936	T11050	T11048	pobj	mice,from
R6937	T11252	T11250	pobj	exposure,to
R6938	T11253	T11248	punct	", ",were
R6939	T11254	T11248	advcl	independent,were
R694	T1358	T1352	punct	: ,results
R6940	T11051	T11050	nmod	Mtf1loxP,mice
R6941	T11255	T11254	prep	of,independent
R6942	T11256	T11257	det	the,presence
R6943	T11257	T11255	pobj	presence,of
R6944	T11264	T11229	punct	.,revealed
R6945	T11258	T11257	cc	or,presence
R6946	T11259	T11257	conj	absence,presence
R6947	T11266	T11267	nsubj	Evidence,is
R6948	T11260	T11257	prep	of,presence
R6949	T11261	T11260	pobj	MTF,of
R695	T1359	T1360	amod	targeted,disruption
R6950	T11262	T11261	punct	-,MTF
R6951	T11263	T11261	nummod	1,MTF
R6952	T11268	T11266	acl	suggests,Evidence
R6953	T11269	T11270	mark	that,is
R6954	T11270	T11268	ccomp	is,suggests
R6955	T11370	T11368	dobj	turnover,has
R6956	T11371	T11372	advmod	extremely,slow
R6957	T11372	T11370	amod	slow,turnover
R6958	T11373	T11374	punct	(,76
R6959	T11374	T11370	parataxis	76,turnover
R696	T1360	T1352	nsubj	disruption,results
R6960	T11271	T11272	det	the,production
R6961	T11375	T11374	nummod	75,76
R6962	T11376	T11374	punct	",",76
R6963	T11377	T11374	punct	),76
R6964	T11272	T11270	nsubj	production,is
R6965	T11378	T11350	punct	.,tends
R6966	T11380	T11381	advmod	Furthermore,provide
R6967	T11273	T11272	prep	of,production
R6968	T11382	T11381	punct	", ",provide
R6969	T11274	T11275	amod	reactive,species
R697	T1361	T1360	prep	of,disruption
R6970	T11383	T11381	nsubj	metallothioneins,provide
R6971	T11384	T11381	dobj	protection,provide
R6972	T11275	T11273	pobj	species,of
R6973	T11385	T11384	prep	against,protection
R6974	T11386	T11387	amod	oxidative,stress
R6975	T11387	T11385	pobj	stress,against
R6976	T11276	T11275	compound	oxygen,species
R6977	T11388	T11389	punct	(,17
R6978	T11389	T11381	parataxis	17,provide
R6979	T11390	T11389	nummod	1,17
R698	T1362	T1361	pobj	Mtf1,of
R6980	T11277	T11278	det	a,effect
R6981	T11391	T11389	punct	",",17
R6982	T11392	T11389	punct	),17
R6983	T11393	T11381	punct	.,provide
R6984	T11278	T11270	attr	effect,is
R6985	T11395	T11396	prep	In,postulated
R6986	T11279	T11278	amod	major,effect
R6987	T11397	T11395	pobj	addition,In
R6988	T11398	T11397	prep	to,addition
R6989	T11280	T11278	prep	of,effect
R699	T1363	T1352	prep	in,results
R6990	T11399	T11398	pobj	metallothioneins,to
R6991	T11400	T11396	punct	", ",postulated
R6992	T11401	T11396	nsubjpass	glutathione,postulated
R6993	T11281	T11282	amod	acute,toxicity
R6994	T11402	T11396	auxpass	was,postulated
R6995	T11282	T11280	pobj	toxicity,of
R6996	T11403	T11396	prep	as,postulated
R6997	T11404	T11405	det	a,line
R6998	T11405	T11403	pobj	line,as
R6999	T11406	T11405	amod	first,line
R7	T242	T240	amod	major,branches
R70	T310	T311	det	a,function
R700	T1364	T1365	amod	embryonic,lethality
R7000	T11283	T11282	compound	cadmium,toxicity
R7001	T11407	T11405	prep	of,line
R7002	T11408	T11407	pobj	defense,of
R7003	T11409	T11408	prep	against,defense
R7004	T11284	T11285	punct	(,69
R7005	T11410	T11411	compound	cadmium,toxicity
R7006	T11411	T11409	pobj	toxicity,against
R7007	T11412	T11413	punct	(,77
R7008	T11285	T11270	parataxis	69,is
R7009	T11413	T11396	parataxis	77,postulated
R701	T1365	T1363	pobj	lethality,in
R7010	T11414	T11413	punct	),77
R7011	T11415	T11396	punct	.,postulated
R7012	T11286	T11285	nummod	68,69
R7013	T11417	T11418	nsubj	Glutathione,complexes
R7014	T11287	T11285	punct	",",69
R7015	T11419	T11418	advmod	efficiently,complexes
R7016	T11420	T11418	dobj	cadmium,complexes
R7017	T11421	T11422	punct	(,78
R7018	T11288	T11285	punct	),69
R7019	T11422	T11418	parataxis	78,complexes
R702	T1366	T1352	prep	around,results
R7020	T11423	T11422	punct	),78
R7021	T11289	T11267	punct	", ",is
R7022	T11424	T11418	cc	and,complexes
R7023	T11425	T11418	conj	scavenges,complexes
R7024	T11426	T11427	amod	free,radicals
R7025	T11290	T11267	cc	and,is
R7026	T11427	T11425	dobj	radicals,scavenges
R7027	T11428	T11427	cc	and,radicals
R7028	T11429	T11430	amod	other,species
R7029	T11291	T11267	nsubj	exposure,is
R703	T1367	T1368	nummod	14,days
R7030	T11430	T11427	conj	species,radicals
R7031	T11431	T11430	amod	reactive,species
R7032	T11432	T11430	compound	oxygen,species
R7033	T11292	T11291	prep	of,exposure
R7034	T11433	T11425	advmod	directly,scavenges
R7035	T11434	T11433	punct	", ",directly
R7036	T11435	T11433	cc	and,directly
R7037	T11293	T11294	amod	cultured,cells
R7038	T11436	T11433	conj	indirectly,directly
R7039	T11437	T11425	prep	via,scavenges
R704	T1368	T1366	pobj	days,around
R7040	T11438	T11439	amod	enzymatic,reactions
R7041	T11294	T11292	pobj	cells,of
R7042	T11439	T11437	pobj	reactions,via
R7043	T11440	T11441	punct	(,45
R7044	T11295	T11294	cc	or,cells
R7045	T11441	T11425	parataxis	45,scavenges
R7046	T11442	T11441	punct	),45
R7047	T11443	T11418	punct	.,complexes
R7048	T11296	T11294	conj	animals,cells
R7049	T11445	T11446	prep	In,oxidized
R705	T1369	T1370	advmod	post,coitum
R7050	T11297	T11291	prep	to,exposure
R7051	T11447	T11448	amod	such,reactions
R7052	T11448	T11445	pobj	reactions,In
R7053	T11298	T11297	pobj	cadmium,to
R7054	T11449	T11446	punct	", ",oxidized
R7055	T11450	T11446	nsubjpass	glutathione,oxidized
R7056	T11451	T11446	auxpass	is,oxidized
R7057	T11299	T11267	acomp	associated,is
R7058	T11452	T11446	cc	and,oxidized
R7059	T11453	T11446	conj	has,oxidized
R706	T1370	T1368	advmod	coitum,days
R7060	T11454	T11455	aux	to,regenerated
R7061	T11455	T11453	xcomp	regenerated,has
R7062	T11456	T11455	auxpass	be,regenerated
R7063	T11457	T11455	agent	by,regenerated
R7064	T11300	T11299	prep	with,associated
R7065	T11458	T11459	compound	glutathione,reductase
R7066	T11459	T11457	pobj	reductase,by
R7067	T11460	T11446	punct	.,oxidized
R7068	T11301	T11300	pobj	depletion,with
R7069	T11462	T11463	advmod	Also,mediate
R707	T1371	T1352	prep	due,results
R7070	T11302	T11301	prep	of,depletion
R7071	T11464	T11463	punct	", ",mediate
R7072	T11465	T11466	compound	glutathione,transferases
R7073	T11466	T11463	nsubj	transferases,mediate
R7074	T11303	T11304	amod	reduced,glutathione
R7075	T11467	T11466	punct	-,transferases
R7076	T11468	T11466	compound	S,transferases
R7077	T11304	T11302	pobj	glutathione,of
R7078	T11469	T11466	punct	-,transferases
R7079	T11470	T11471	det	the,conjugation
R708	T1372	T1371	pcomp	to,due
R7080	T11471	T11463	dobj	conjugation,mediate
R7081	T11305	T11304	punct	", ",glutathione
R7082	T11472	T11471	prep	of,conjugation
R7083	T11473	T11474	amod	various,electrophiles
R7084	T11474	T11472	pobj	electrophiles,of
R7085	T11306	T11307	compound	lipid,peroxidation
R7086	T11307	T11304	conj	peroxidation,glutathione
R7087	T11308	T11307	cc	and,peroxidation
R7088	T11309	T11310	compound	DNA,damage
R7089	T11475	T11471	prep	to,conjugation
R709	T1373	T1374	compound	hepatocyte,necrosis
R7090	T11310	T11307	conj	damage,peroxidation
R7091	T11311	T11312	punct	(,70
R7092	T11476	T11475	pobj	glutathione,to
R7093	T11312	T11310	parataxis	70,damage
R7094	T11477	T11463	punct	.,mediate
R7095	T11313	T11314	punct	–,73
R7096	T11479	T11480	det	The,upregulation
R7097	T11480	T11485	nsubj	upregulation,supports
R7098	T11314	T11312	prep	73,70
R7099	T11481	T11480	amod	observed,upregulation
R71	T311	T307	dobj	function,serves
R710	T1374	T1371	pobj	necrosis,due
R7100	T11482	T11483	npadvmod	cadmium,induced
R7101	T11483	T11480	amod	induced,upregulation
R7102	T11315	T11312	punct	),70
R7103	T11484	T11483	punct	-,induced
R7104	T11486	T11480	prep	of,upregulation
R7105	T11316	T11267	punct	.,is
R7106	T11487	T11486	pobj	Gclc,of
R7107	T11488	T11487	punct	", ",Gclc
R7108	T11318	T11319	amod	Oxidative,stress
R7109	T11489	T11487	conj	Gsr,Gclc
R711	T1375	T1376	punct	(,23
R7110	T11490	T11489	cc	and,Gsr
R7111	T11491	T11489	conj	Gstm4,Gsr
R7112	T11492	T11493	det	the,importance
R7113	T11493	T11485	dobj	importance,supports
R7114	T11494	T11493	prep	of,importance
R7115	T11495	T11494	pobj	glutathione,of
R7116	T11319	T11320	nsubjpass	stress,shown
R7117	T11496	T11493	prep	in,importance
R7118	T11497	T11498	det	the,response
R7119	T11498	T11496	pobj	response,in
R712	T1376	T1352	parataxis	23,results
R7120	T11321	T11319	cc	and,stress
R7121	T11499	T11498	amod	cellular,response
R7122	T11500	T11498	compound	cadmium,response
R7123	T11501	T11485	punct	.,supports
R7124	T11322	T11323	det	the,restoration
R7125	T11323	T11319	conj	restoration,stress
R7126	T11503	T11504	det	The,sensitivity
R7127	T11504	T11506	nsubj	sensitivity,corroborates
R7128	T11324	T11323	amod	subsequent,restoration
R7129	T11505	T11504	amod	enhanced,sensitivity
R713	T1377	T1376	punct	),23
R7130	T11325	T11323	prep	of,restoration
R7131	T11507	T11504	prep	to,sensitivity
R7132	T11508	T11509	compound	cadmium,toxicity
R7133	T11509	T11507	pobj	toxicity,to
R7134	T11326	T11327	amod	cellular,homeostasis
R7135	T11510	T11511	dep	that,found
R7136	T11511	T11504	relcl	found,sensitivity
R7137	T11512	T11511	nsubj	we,found
R7138	T11327	T11325	pobj	homeostasis,of
R7139	T11513	T11511	prep	for,found
R714	T1378	T1352	punct	.,results
R7140	T11514	T11515	nmod	mouse,fibroblasts
R7141	T11328	T11320	aux	have,shown
R7142	T11515	T11513	pobj	fibroblasts,for
R7143	T11516	T11515	amod	embryonic,fibroblasts
R7144	T11517	T11511	prep	upon,found
R7145	T11329	T11320	auxpass	been,shown
R7146	T11518	T11519	det	a,combination
R7147	T11519	T11517	pobj	combination,upon
R7148	T11520	T11519	prep	of,combination
R7149	T11330	T11331	aux	to,induce
R715	T1380	T1381	prep	In,are
R7150	T11521	T11522	compound	Mtf1,deletion
R7151	T11522	T11520	pobj	deletion,of
R7152	T11331	T11320	xcomp	induce,shown
R7153	T11523	T11522	cc	and,deletion
R7154	T11524	T11522	conj	depletion,deletion
R7155	T11525	T11524	prep	of,depletion
R7156	T11332	T11333	det	the,expression
R7157	T11526	T11525	pobj	glutathione,of
R7158	T11527	T11506	advmod	further,corroborates
R7159	T11528	T11529	det	the,importance
R716	T1382	T1380	pobj	contrast,In
R7160	T11333	T11331	dobj	expression,induce
R7161	T11529	T11506	dobj	importance,corroborates
R7162	T11530	T11529	prep	of,importance
R7163	T11531	T11530	pobj	MTF,of
R7164	T11532	T11531	punct	-,MTF
R7165	T11533	T11531	nummod	1,MTF
R7166	T11334	T11333	prep	of,expression
R7167	T11534	T11531	cc	and,MTF
R7168	T11535	T11536	poss	its,genes
R7169	T11536	T11531	conj	genes,MTF
R717	T1383	T1381	punct	", ",are
R7170	T11335	T11334	pobj	genes,of
R7171	T11537	T11536	compound	target,genes
R7172	T11538	T11539	advmod	as,as
R7173	T11539	T11531	cc	as,MTF
R7174	T11336	T11335	acl	encoding,genes
R7175	T11540	T11539	advmod	well,as
R7176	T11337	T11338	amod	acute,phase
R7177	T11541	T11542	amod	reduced,glutathione
R7178	T11338	T11340	compound	phase,proteins
R7179	T11542	T11531	conj	glutathione,MTF
R718	T1384	T1381	nsubj	mice,are
R7180	T11543	T11529	prep	for,importance
R7181	T11544	T11545	det	an,defense
R7182	T11339	T11338	punct	-,phase
R7183	T11545	T11543	pobj	defense,for
R7184	T11546	T11545	amod	efficient,defense
R7185	T11547	T11545	amod	anti-cadmium,defense
R7186	T11340	T11336	dobj	proteins,encoding
R7187	T11548	T11506	punct	.,corroborates
R7188	T11341	T11340	cc	and,proteins
R7189	T11550	T11551	poss	Our,data
R719	T1385	T1384	prep	with,mice
R7190	T11551	T11552	nsubj	data,provide
R7191	T11342	T11343	amod	antioxidant,enzymes
R7192	T11553	T11554	amod	strong,evidence
R7193	T11554	T11552	dobj	evidence,provide
R7194	T11555	T11552	prep	for,provide
R7195	T11343	T11340	conj	enzymes,proteins
R7196	T11556	T11557	advmod	at,two
R7197	T11557	T11559	nummod	two,branches
R7198	T11558	T11557	advmod	least,two
R7199	T11344	T11345	punct	(,74
R72	T312	T311	amod	vital,function
R720	T1386	T1387	amod	null,mutations
R7200	T11559	T11555	pobj	branches,for
R7201	T11560	T11559	prep	of,branches
R7202	T11561	T11562	amod	cellular,defense
R7203	T11345	T11331	parataxis	74,induce
R7204	T11562	T11560	pobj	defense,of
R7205	T11563	T11562	amod	anti-cadmium,defense
R7206	T11564	T11559	punct	", ",branches
R7207	T11346	T11345	punct	),74
R7208	T11565	T11559	appos	one,branches
R7209	T11566	T11565	prep	via,one
R721	T1387	T1385	pobj	mutations,with
R7210	T11567	T11566	pobj	MTF,via
R7211	T11347	T11320	punct	.,shown
R7212	T11568	T11567	punct	-,MTF
R7213	T11569	T11567	nummod	1,MTF
R7214	T11570	T11567	cc	and,MTF
R7215	T11349	T11350	prep	In,tends
R7216	T11571	T11572	poss	its,genes
R7217	T11572	T11567	conj	genes,MTF
R7218	T11573	T11572	compound	target,genes
R7219	T11574	T11572	punct	", ",genes
R722	T1388	T1387	prep	for,mutations
R7220	T11575	T11576	advmod	notably,metallothioneins
R7221	T11576	T11572	appos	metallothioneins,genes
R7222	T11351	T11349	pobj	mammals,In
R7223	T11577	T11565	punct	", ",one
R7224	T11578	T11579	det	the,other
R7225	T11579	T11565	appos	other,one
R7226	T11580	T11579	prep	via,other
R7227	T11352	T11350	punct	", ",tends
R7228	T11353	T11350	nsubj	cadmium,tends
R7229	T11581	T11580	pobj	glutathione,via
R723	T1389	T1390	det	the,genes
R7230	T11582	T11579	punct	", ",other
R7231	T11354	T11355	aux	to,accumulate
R7232	T11583	T11579	prep	with,other
R7233	T11584	T11585	det	an,overlap
R7234	T11585	T11583	pobj	overlap,with
R7235	T11355	T11350	xcomp	accumulate,tends
R7236	T11586	T11585	amod	apparent,overlap
R7237	T11356	T11355	prep	in,accumulate
R7238	T11587	T11585	prep	in,overlap
R7239	T11357	T11358	det	the,kidney
R724	T1390	T1388	pobj	genes,for
R7240	T11588	T11587	pobj	Sepw1,in
R7241	T11589	T11552	punct	.,provide
R7242	T11358	T11356	pobj	kidney,in
R7243	T11359	T11358	cc	and,kidney
R7244	T11360	T11358	conj	liver,kidney
R7245	T11361	T11355	prep	as,accumulate
R7246	T11362	T11363	det	a,complex
R7247	T11363	T11361	pobj	complex,as
R7248	T11364	T11365	compound	cadmium,metallothionein
R7249	T11365	T11363	compound	metallothionein,complex
R725	T1391	T1392	npadvmod	stress,inducible
R7250	T11366	T11365	punct	-,metallothionein
R7251	T11367	T11368	dep	that,has
R7252	T11368	T11363	relcl	has,complex
R7253	T11369	T11370	det	an,turnover
R726	T1392	T1390	amod	inducible,genes
R727	T1393	T1392	punct	-,inducible
R728	T1394	T1390	compound	metallothionein,genes
R729	T1395	T1390	punct	(,genes
R73	T313	T307	prep	in,serves
R730	T1396	T1390	appos	Mt1,genes
R731	T1397	T1396	cc	and,Mt1
R732	T1398	T1396	conj	Mt2,Mt1
R733	T1399	T1381	punct	),are
R734	T1400	T1381	acomp	viable,are
R735	T1401	T1381	punct	", ",are
R736	T1402	T1403	mark	though,sensitive
R737	T1403	T1381	advcl	sensitive,are
R738	T1404	T1403	prep	to,sensitive
R739	T1405	T1404	pobj	cadmium,to
R74	T314	T315	amod	embryonic,development
R740	T1406	T1407	punct	(,25
R741	T1407	T1403	parataxis	25,sensitive
R742	T1408	T1407	nummod	24,25
R743	T1409	T1407	punct	",",25
R744	T1410	T1407	punct	),25
R745	T1411	T1381	punct	", ",are
R746	T1412	T1381	advcl	indicating,are
R747	T1413	T1414	mark	that,involved
R748	T1414	T1412	ccomp	involved,indicating
R749	T1415	T1416	amod	additional,genes
R75	T315	T313	pobj	development,in
R750	T1416	T1414	nsubjpass	genes,involved
R751	T1417	T1416	amod	important,genes
R752	T1418	T1416	nmod	MTF,genes
R753	T1419	T1418	punct	-,MTF
R754	T1420	T1418	nummod	1,MTF
R755	T1421	T1416	compound	target,genes
R756	T1422	T1414	auxpass	are,involved
R757	T1423	T1414	prep	in,involved
R758	T1424	T1425	det	the,phenotype
R759	T1425	T1423	pobj	phenotype,in
R76	T316	T315	compound	liver,development
R760	T1426	T1425	amod	lethal,phenotype
R761	T1427	T1381	punct	.,are
R762	T1429	T1430	prep	With,is
R763	T1431	T1432	det	the,technique
R764	T1432	T1429	pobj	technique,With
R765	T1433	T1434	nmod	Cre,loxP
R766	T1434	T1432	nmod	loxP,technique
R767	T1435	T1434	punct	-,loxP
R768	T1436	T1437	amod	conditional,knockout
R769	T1437	T1432	compound	knockout,technique
R77	T317	T307	punct	.,serves
R770	T1438	T1430	punct	", ",is
R771	T1439	T1430	nsubj	it,is
R772	T1440	T1430	acomp	possible,is
R773	T1441	T1442	aux	to,circumvent
R774	T1442	T1430	xcomp	circumvent,is
R775	T1443	T1444	det	the,phenotype
R776	T1444	T1442	dobj	phenotype,circumvent
R777	T1445	T1446	amod	embryonic,lethal
R778	T1446	T1444	amod	lethal,phenotype
R779	T1447	T1444	prep	of,phenotype
R78	T319	T320	advmod	However,is
R780	T1448	T1449	amod	conventional,mice
R781	T1449	T1447	pobj	mice,of
R782	T1450	T1451	compound	Mtf1,knockout
R783	T1451	T1449	compound	knockout,mice
R784	T1452	T1430	punct	.,is
R785	T1454	T1455	amod	Previous,experiments
R786	T1455	T1456	nsubj	experiments,revealed
R787	T1457	T1455	prep	with,experiments
R788	T1458	T1459	det	this,technique
R789	T1459	T1457	pobj	technique,with
R79	T321	T320	punct	", ",is
R790	T1460	T1461	mark	that,is
R791	T1461	T1456	ccomp	is,revealed
R792	T1462	T1461	nsubj	deletion,is
R793	T1463	T1462	prep	of,deletion
R794	T1464	T1463	pobj	Mtf1,of
R795	T1465	T1462	prep	from,deletion
R796	T1466	T1467	det	the,liver
R797	T1467	T1465	pobj	liver,from
R798	T1468	T1462	prep	after,deletion
R799	T1469	T1468	pobj	birth,after
R8	T243	T240	prep	of,branches
R80	T322	T323	amod	targeted,deletion
R800	T1470	T1471	neg	no,longer
R801	T1471	T1461	advmod	longer,is
R802	T1472	T1461	acomp	lethal,is
R803	T1473	T1461	prep	under,is
R804	T1474	T1475	amod	non-stress,conditions
R805	T1475	T1473	pobj	conditions,under
R806	T1476	T1477	punct	(,26
R807	T1477	T1461	parataxis	26,is
R808	T1478	T1477	punct	),26
R809	T1479	T1456	punct	.,revealed
R81	T323	T320	nsubj	deletion,is
R810	T1481	T1482	prep	For,generated
R811	T1483	T1484	det	this,study
R812	T1484	T1481	pobj	study,For
R813	T1485	T1482	punct	", ",generated
R814	T1486	T1487	det	an,line
R815	T1487	T1482	nsubjpass	line,generated
R816	T1488	T1487	amod	inducible,line
R817	T1489	T1487	punct	", ",line
R818	T1490	T1491	npadvmod	liver,specific
R819	T1491	T1487	amod	specific,line
R82	T324	T323	prep	of,deletion
R820	T1492	T1491	punct	-,specific
R821	T1493	T1494	compound	Mtf1,knockout
R822	T1494	T1487	compound	knockout,line
R823	T1495	T1487	compound	mouse,line
R824	T1496	T1482	auxpass	was,generated
R825	T1497	T1498	aux	to,perform
R826	T1498	T1482	advcl	perform,generated
R827	T1499	T1500	det	a,search
R828	T1500	T1498	dobj	search,perform
R829	T1501	T1500	prep	for,search
R83	T325	T324	pobj	Mtf1,of
R830	T1502	T1503	nmod	MTF,genes
R831	T1503	T1501	pobj	genes,for
R832	T1504	T1502	punct	-,MTF
R833	T1505	T1502	nummod	1,MTF
R834	T1506	T1503	compound	target,genes
R835	T1507	T1503	cc	and,genes
R836	T1508	T1509	npadvmod	cadmium,inducible
R837	T1509	T1511	amod	inducible,genes
R838	T1510	T1509	punct	-,inducible
R839	T1511	T1503	conj	genes,genes
R84	T326	T323	prep	in,deletion
R840	T1512	T1498	prep	in,perform
R841	T1513	T1514	det	the,liver
R842	T1514	T1512	pobj	liver,in
R843	T1515	T1514	amod	adult,liver
R844	T1516	T1482	punct	.,generated
R845	T1518	T1519	det	A,number
R846	T1519	T1520	nsubj	number,emerged
R847	T1521	T1519	prep	of,number
R848	T1522	T1523	compound	target,gene
R849	T1523	T1524	compound	gene,candidates
R85	T327	T328	det	the,liver
R850	T1524	T1521	pobj	candidates,of
R851	T1525	T1520	prep	upon,emerged
R852	T1526	T1527	det	a,analysis
R853	T1527	T1525	pobj	analysis,upon
R854	T1528	T1527	compound	transcriptome,analysis
R855	T1529	T1527	prep	of,analysis
R856	T1530	T1531	amod	mock,treated
R857	T1531	T1536	amod	treated,mice
R858	T1532	T1530	punct	-,mock
R859	T1533	T1530	cc	and,mock
R86	T328	T326	pobj	liver,in
R860	T1534	T1530	conj	cadmium,mock
R861	T1535	T1531	punct	-,treated
R862	T1536	T1529	pobj	mice,of
R863	T1537	T1536	nmod	Mtf1,mice
R864	T1538	T1539	amod	conditional,knockout
R865	T1539	T1536	compound	knockout,mice
R866	T1540	T1536	cc	and,mice
R867	T1541	T1542	compound	control,littermates
R868	T1542	T1536	conj	littermates,mice
R869	T1543	T1520	cc	and,emerged
R87	T329	T323	prep	after,deletion
R870	T1544	T1545	nsubjpass	several,confirmed
R871	T1545	T1520	conj	confirmed,emerged
R872	T1546	T1544	prep	of,several
R873	T1547	T1546	pobj	these,of
R874	T1548	T1545	auxpass	were,confirmed
R875	T1549	T1545	agent	by,confirmed
R876	T1550	T1551	amod	semiquantitative,PCR
R877	T1551	T1549	pobj	PCR,by
R878	T1552	T1551	compound	RT,PCR
R879	T1553	T1551	punct	–,PCR
R88	T330	T329	pobj	birth,after
R880	T1554	T1545	punct	.,confirmed
R881	T1556	T1557	prep	Besides,find
R882	T1558	T1559	det	the,genes
R883	T1559	T1556	pobj	genes,Besides
R884	T1560	T1561	npadvmod	stress,inducible
R885	T1561	T1559	amod	inducible,genes
R886	T1562	T1561	punct	-,inducible
R887	T1563	T1559	compound	metallothionein,genes
R888	T1564	T1565	dep	that,known
R889	T1565	T1559	relcl	known,genes
R89	T331	T332	neg	no,longer
R890	T1566	T1565	auxpass	were,known
R891	T1567	T1565	advmod	already,known
R892	T1568	T1565	prep	as,known
R893	T1569	T1570	compound	target,genes
R894	T1570	T1568	pobj	genes,as
R895	T1571	T1570	prep	of,genes
R896	T1572	T1571	pobj	MTF,of
R897	T1573	T1572	punct	-,MTF
R898	T1574	T1572	nummod	1,MTF
R899	T1575	T1557	punct	", ",find
R9	T244	T245	amod	anti-cadmium,defense
R90	T332	T320	advmod	longer,is
R900	T1576	T1557	nsubj	we,find
R901	T1577	T1578	mark	that,is
R902	T1578	T1557	ccomp	is,find
R903	T1579	T1578	nsubj	MTF,is
R904	T1580	T1579	punct	-,MTF
R905	T1581	T1579	nummod	1,MTF
R906	T1582	T1578	acomp	important,is
R907	T1583	T1582	prep	for,important
R908	T1584	T1585	amod	basal,expression
R909	T1585	T1583	pobj	expression,for
R91	T333	T320	acomp	lethal,is
R910	T1586	T1585	compound	liver,expression
R911	T1587	T1585	prep	of,expression
R912	T1588	T1589	det	the,gene
R913	T1589	T1587	pobj	gene,of
R914	T1590	T1589	prep	for,gene
R915	T1591	T1592	nmod	selenoprotein,muscle
R916	T1592	T1590	pobj	muscle,for
R917	T1593	T1592	nmod	W,muscle
R918	T1594	T1592	punct	", ",muscle
R919	T1595	T1592	nummod	1,muscle
R92	T334	T320	punct	.,is
R920	T1596	T1592	punct	(,muscle
R921	T1597	T1592	appos	Sepw1,muscle
R922	T1598	T1590	punct	),for
R923	T1599	T1600	advmod	as,as
R924	T1600	T1590	cc	as,for
R925	T1601	T1600	advmod	well,as
R926	T1602	T1590	conj	for,for
R927	T1603	T1604	npadvmod	cadmium,induced
R928	T1604	T1606	amod	induced,expression
R929	T1605	T1604	punct	-,induced
R93	T336	T337	prep	For,treated
R930	T1606	T1602	pobj	expression,for
R931	T1607	T1606	prep	of,expression
R932	T1608	T1609	nmod	N,myc
R933	T1609	T1611	nmod	myc,gene
R934	T1610	T1609	punct	-,myc
R935	T1611	T1607	pobj	gene,of
R936	T1612	T1613	advmod	downstream,regulated
R937	T1613	T1611	amod	regulated,gene
R938	T1614	T1611	nummod	1,gene
R939	T1615	T1611	punct	(,gene
R94	T338	T339	det	this,study
R940	T1616	T1611	appos	Ndrg1,gene
R941	T1617	T1611	punct	),gene
R942	T1618	T1611	cc	and,gene
R943	T1619	T1620	det	the,gene
R944	T1620	T1611	conj	gene,gene
R945	T1621	T1620	acl	encoding,gene
R946	T1622	T1623	npadvmod	cysteine,rich
R947	T1623	T1628	amod	rich,protein
R948	T1624	T1622	punct	-,cysteine
R949	T1625	T1622	cc	and,cysteine
R95	T339	T336	pobj	study,For
R950	T1626	T1622	conj	glycine,cysteine
R951	T1627	T1623	punct	-,rich
R952	T1628	T1621	dobj	protein,encoding
R953	T1629	T1628	nummod	1,protein
R954	T1630	T1628	punct	(,protein
R955	T1631	T1628	appos	Csrp1,protein
R956	T1632	T1557	punct	),find
R957	T1633	T1557	punct	.,find
R958	T1635	T1636	prep	In,appears
R959	T1637	T1635	pobj	addition,In
R96	T340	T337	punct	", ",treated
R960	T1638	T1636	punct	", ",appears
R961	T1639	T1636	nsubj	MTF,appears
R962	T1640	T1639	punct	-,MTF
R963	T1641	T1639	nummod	1,MTF
R964	T1642	T1643	aux	to,repress
R965	T1643	T1636	xcomp	repress,appears
R966	T1644	T1645	det	the,expression
R967	T1645	T1643	dobj	expression,repress
R968	T1646	T1645	prep	of,expression
R969	T1647	T1648	nmod	solute,member
R97	T341	T342	nmod	Mtf1,mice
R970	T1648	T1653	nmod	member,gene
R971	T1649	T1648	nmod	carrier,member
R972	T1650	T1648	nmod	family,member
R973	T1651	T1648	nummod	39,member
R974	T1652	T1648	punct	", ",member
R975	T1653	T1646	pobj	gene,of
R976	T1654	T1648	nummod	10,member
R977	T1655	T1653	punct	(,gene
R978	T1656	T1653	appos	Slc39a10,gene
R979	T1657	T1653	punct	),gene
R98	T342	T337	nsubjpass	mice,treated
R980	T1658	T1653	punct	", ",gene
R981	T1659	T1660	dep	which,encodes
R982	T1660	T1653	relcl	encodes,gene
R983	T1661	T1662	det	a,transporter
R984	T1662	T1660	dobj	transporter,encodes
R985	T1663	T1662	amod	putative,transporter
R986	T1664	T1665	compound	metal,ion
R987	T1665	T1662	compound	ion,transporter
R988	T1666	T1636	punct	.,appears
R989	T1668	T1669	prep	In,induced
R99	T343	T344	amod	conditional,knockout
R990	T1670	T1671	det	an,response
R991	T1671	T1668	pobj	response,In
R992	T1672	T1673	npadvmod	MTF,independent
R993	T1673	T1671	amod	independent,response
R994	T1674	T1672	punct	-,MTF
R995	T1675	T1672	nummod	1,MTF
R996	T1676	T1673	punct	-,independent
R997	T1677	T1671	compound	transcriptome,response
R998	T1678	T1669	punct	", ",induced
R999	T1679	T1680	amod	several,genes

PMC:1253828 JSONTXT 10 Projects

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PMC:1253828 JSON TXT 10 Projects