Id |
Subject |
Object |
Predicate |
Lexical cue |
T2350 |
0-2 |
PRP |
denotes |
We |
T2351 |
3-15 |
VBD |
denotes |
hypothesised |
T2352 |
16-20 |
IN |
denotes |
that |
T2354 |
21-24 |
DT |
denotes |
the |
T2356 |
25-26 |
NN |
denotes |
C |
T2358 |
26-27 |
HYPH |
denotes |
- |
T2357 |
27-35 |
JJ |
denotes |
terminal |
T2355 |
36-43 |
NN |
denotes |
peptide |
T2359 |
44-49 |
MD |
denotes |
might |
T2360 |
50-52 |
VB |
denotes |
be |
T2353 |
53-61 |
VBN |
denotes |
involved |
T2361 |
62-64 |
IN |
denotes |
in |
T2362 |
65-72 |
NN |
denotes |
protein |
T2364 |
72-73 |
SYM |
denotes |
- |
T2363 |
73-80 |
NN |
denotes |
protein |
T2365 |
81-93 |
NNS |
denotes |
interactions |
T2366 |
93-95 |
, |
denotes |
, |
T2367 |
95-98 |
CC |
denotes |
and |
T2368 |
99-101 |
PRP |
denotes |
we |
T2369 |
102-108 |
VBD |
denotes |
tested |
T2370 |
109-113 |
DT |
denotes |
this |
T2371 |
114-116 |
IN |
denotes |
by |
T2372 |
117-126 |
VBG |
denotes |
screening |
T2373 |
127-128 |
DT |
denotes |
a |
T2375 |
129-133 |
NN |
denotes |
cDNA |
T2374 |
134-141 |
NN |
denotes |
library |
T2376 |
142-147 |
VBG |
denotes |
using |
T2377 |
148-151 |
DT |
denotes |
the |
T2379 |
152-157 |
NN |
denotes |
yeast |
T2380 |
158-161 |
CD |
denotes |
two |
T2382 |
161-162 |
HYPH |
denotes |
- |
T2381 |
162-168 |
NN |
denotes |
hybrid |
T2378 |
169-175 |
NN |
denotes |
system |
T2383 |
176-180 |
IN |
denotes |
with |
T2384 |
181-182 |
DT |
denotes |
a |
T2385 |
183-192 |
NN |
denotes |
construct |
T2386 |
193-194 |
-LRB- |
denotes |
( |
T2387 |
194-201 |
NN |
denotes |
PAX6CTP |
T2388 |
201-202 |
-RRB- |
denotes |
) |
T2389 |
203-205 |
IN |
denotes |
in |
T2391 |
206-211 |
WDT |
denotes |
which |
T2392 |
212-215 |
DT |
denotes |
the |
T2394 |
216-218 |
CD |
denotes |
32 |
T2396 |
219-224 |
NN |
denotes |
amino |
T2395 |
225-229 |
NN |
denotes |
acid |
T2397 |
230-231 |
NN |
denotes |
C |
T2399 |
231-232 |
HYPH |
denotes |
- |
T2398 |
232-240 |
JJ |
denotes |
terminal |
T2393 |
241-248 |
NN |
denotes |
peptide |
T2400 |
249-252 |
VBD |
denotes |
was |
T2390 |
253-258 |
VBN |
denotes |
fused |
T2401 |
259-261 |
IN |
denotes |
to |
T2402 |
262-265 |
DT |
denotes |
the |
T2404 |
266-271 |
NN |
denotes |
yeast |
T2405 |
272-276 |
NN |
denotes |
GAL4 |
T2406 |
277-280 |
NN |
denotes |
DNA |
T2407 |
281-288 |
NN |
denotes |
binding |
T2403 |
289-295 |
NN |
denotes |
domain |
T2408 |
295-296 |
. |
denotes |
. |
T2409 |
296-380 |
sentence |
denotes |
We chose to screen a mouse brain cDNA library as no human libraries were available. |
T2410 |
297-299 |
PRP |
denotes |
We |
T2411 |
300-305 |
VBD |
denotes |
chose |
T2412 |
306-308 |
TO |
denotes |
to |
T2413 |
309-315 |
VB |
denotes |
screen |
T2414 |
316-317 |
DT |
denotes |
a |
T2416 |
318-323 |
NN |
denotes |
mouse |
T2417 |
324-329 |
NN |
denotes |
brain |
T2418 |
330-334 |
NN |
denotes |
cDNA |
T2415 |
335-342 |
NN |
denotes |
library |
T2419 |
343-345 |
IN |
denotes |
as |
T2421 |
346-348 |
DT |
denotes |
no |
T2423 |
349-354 |
JJ |
denotes |
human |
T2422 |
355-364 |
NNS |
denotes |
libraries |
T2420 |
365-369 |
VBD |
denotes |
were |
T2424 |
370-379 |
JJ |
denotes |
available |
T2425 |
379-380 |
. |
denotes |
. |
T2426 |
380-548 |
sentence |
denotes |
Given the fact that the amino acid sequence of the PAX6 protein is identical in man and mouse, we reasoned that a mouse brain library would yield relevant interactors. |
T2427 |
381-386 |
VBN |
denotes |
Given |
T2429 |
387-390 |
DT |
denotes |
the |
T2430 |
391-395 |
NN |
denotes |
fact |
T2431 |
396-400 |
IN |
denotes |
that |
T2433 |
401-404 |
DT |
denotes |
the |
T2435 |
405-410 |
NN |
denotes |
amino |
T2436 |
411-415 |
NN |
denotes |
acid |
T2434 |
416-424 |
NN |
denotes |
sequence |
T2437 |
425-427 |
IN |
denotes |
of |
T2438 |
428-431 |
DT |
denotes |
the |
T2440 |
432-436 |
NN |
denotes |
PAX6 |
T2439 |
437-444 |
NN |
denotes |
protein |
T2432 |
445-447 |
VBZ |
denotes |
is |
T2441 |
448-457 |
JJ |
denotes |
identical |
T2442 |
458-460 |
IN |
denotes |
in |
T2443 |
461-464 |
NN |
denotes |
man |
T2444 |
465-468 |
CC |
denotes |
and |
T2445 |
469-474 |
NN |
denotes |
mouse |
T2446 |
474-476 |
, |
denotes |
, |
T2447 |
476-478 |
PRP |
denotes |
we |
T2428 |
479-487 |
VBD |
denotes |
reasoned |
T2448 |
488-492 |
IN |
denotes |
that |
T2450 |
493-494 |
DT |
denotes |
a |
T2452 |
495-500 |
NN |
denotes |
mouse |
T2453 |
501-506 |
NN |
denotes |
brain |
T2451 |
507-514 |
NN |
denotes |
library |
T2454 |
515-520 |
MD |
denotes |
would |
T2449 |
521-526 |
VB |
denotes |
yield |
T2455 |
527-535 |
JJ |
denotes |
relevant |
T2456 |
536-547 |
NNS |
denotes |
interactors |
T2457 |
547-548 |
. |
denotes |
. |
T2458 |
548-655 |
sentence |
denotes |
For comparison we also carried out the screen using a construct containing the whole PST domain (PAX6PST). |
T2459 |
549-552 |
IN |
denotes |
For |
T2461 |
553-563 |
NN |
denotes |
comparison |
T2462 |
564-566 |
PRP |
denotes |
we |
T2463 |
567-571 |
RB |
denotes |
also |
T2460 |
572-579 |
VBD |
denotes |
carried |
T2464 |
580-583 |
RP |
denotes |
out |
T2465 |
584-587 |
DT |
denotes |
the |
T2466 |
588-594 |
NN |
denotes |
screen |
T2467 |
595-600 |
VBG |
denotes |
using |
T2468 |
601-602 |
DT |
denotes |
a |
T2469 |
603-612 |
NN |
denotes |
construct |
T2470 |
613-623 |
VBG |
denotes |
containing |
T2471 |
624-627 |
DT |
denotes |
the |
T2473 |
628-633 |
JJ |
denotes |
whole |
T2474 |
634-637 |
NN |
denotes |
PST |
T2472 |
638-644 |
NN |
denotes |
domain |
T2475 |
645-646 |
-LRB- |
denotes |
( |
T2476 |
646-653 |
NN |
denotes |
PAX6PST |
T2477 |
653-654 |
-RRB- |
denotes |
) |
T2478 |
654-655 |
. |
denotes |
. |
R1369 |
T2350 |
T2351 |
nsubj |
We,hypothesised |
R1370 |
T2352 |
T2353 |
mark |
that,involved |
R1371 |
T2353 |
T2351 |
ccomp |
involved,hypothesised |
R1372 |
T2354 |
T2355 |
det |
the,peptide |
R1373 |
T2355 |
T2353 |
nsubjpass |
peptide,involved |
R1374 |
T2356 |
T2357 |
npadvmod |
C,terminal |
R1375 |
T2357 |
T2355 |
amod |
terminal,peptide |
R1376 |
T2358 |
T2357 |
punct |
-,terminal |
R1377 |
T2359 |
T2353 |
aux |
might,involved |
R1378 |
T2360 |
T2353 |
auxpass |
be,involved |
R1379 |
T2361 |
T2353 |
prep |
in,involved |
R1380 |
T2362 |
T2363 |
nmod |
protein,protein |
R1381 |
T2363 |
T2365 |
compound |
protein,interactions |
R1382 |
T2364 |
T2363 |
punct |
-,protein |
R1383 |
T2365 |
T2361 |
pobj |
interactions,in |
R1384 |
T2366 |
T2351 |
punct |
", ",hypothesised |
R1385 |
T2367 |
T2351 |
cc |
and,hypothesised |
R1386 |
T2368 |
T2369 |
nsubj |
we,tested |
R1387 |
T2369 |
T2351 |
conj |
tested,hypothesised |
R1388 |
T2370 |
T2369 |
dobj |
this,tested |
R1389 |
T2371 |
T2369 |
prep |
by,tested |
R1390 |
T2372 |
T2371 |
pcomp |
screening,by |
R1391 |
T2373 |
T2374 |
det |
a,library |
R1392 |
T2374 |
T2372 |
dobj |
library,screening |
R1393 |
T2375 |
T2374 |
compound |
cDNA,library |
R1394 |
T2376 |
T2372 |
advcl |
using,screening |
R1395 |
T2377 |
T2378 |
det |
the,system |
R1396 |
T2378 |
T2376 |
dobj |
system,using |
R1397 |
T2379 |
T2378 |
nmod |
yeast,system |
R1398 |
T2380 |
T2381 |
nummod |
two,hybrid |
R1399 |
T2381 |
T2378 |
compound |
hybrid,system |
R1400 |
T2382 |
T2381 |
punct |
-,hybrid |
R1401 |
T2383 |
T2378 |
prep |
with,system |
R1402 |
T2384 |
T2385 |
det |
a,construct |
R1403 |
T2385 |
T2383 |
pobj |
construct,with |
R1404 |
T2386 |
T2387 |
punct |
(,PAX6CTP |
R1405 |
T2387 |
T2385 |
parataxis |
PAX6CTP,construct |
R1406 |
T2388 |
T2387 |
punct |
),PAX6CTP |
R1407 |
T2389 |
T2390 |
prep |
in,fused |
R1408 |
T2390 |
T2385 |
relcl |
fused,construct |
R1409 |
T2391 |
T2389 |
pobj |
which,in |
R1410 |
T2392 |
T2393 |
det |
the,peptide |
R1411 |
T2393 |
T2390 |
nsubjpass |
peptide,fused |
R1412 |
T2394 |
T2395 |
nummod |
32,acid |
R1413 |
T2395 |
T2393 |
nmod |
acid,peptide |
R1414 |
T2396 |
T2395 |
nmod |
amino,acid |
R1415 |
T2397 |
T2398 |
npadvmod |
C,terminal |
R1416 |
T2398 |
T2393 |
amod |
terminal,peptide |
R1417 |
T2399 |
T2398 |
punct |
-,terminal |
R1418 |
T2400 |
T2390 |
auxpass |
was,fused |
R1419 |
T2401 |
T2390 |
prep |
to,fused |
R1420 |
T2402 |
T2403 |
det |
the,domain |
R1421 |
T2403 |
T2401 |
pobj |
domain,to |
R1422 |
T2404 |
T2403 |
compound |
yeast,domain |
R1423 |
T2405 |
T2403 |
compound |
GAL4,domain |
R1424 |
T2406 |
T2407 |
compound |
DNA,binding |
R1425 |
T2407 |
T2403 |
compound |
binding,domain |
R1426 |
T2408 |
T2369 |
punct |
.,tested |
R1427 |
T2410 |
T2411 |
nsubj |
We,chose |
R1428 |
T2412 |
T2413 |
aux |
to,screen |
R1429 |
T2413 |
T2411 |
xcomp |
screen,chose |
R1430 |
T2414 |
T2415 |
det |
a,library |
R1431 |
T2415 |
T2413 |
dobj |
library,screen |
R1432 |
T2416 |
T2417 |
compound |
mouse,brain |
R1433 |
T2417 |
T2415 |
compound |
brain,library |
R1434 |
T2418 |
T2415 |
compound |
cDNA,library |
R1435 |
T2419 |
T2420 |
mark |
as,were |
R1436 |
T2420 |
T2411 |
advcl |
were,chose |
R1437 |
T2421 |
T2422 |
det |
no,libraries |
R1438 |
T2422 |
T2420 |
nsubj |
libraries,were |
R1439 |
T2423 |
T2422 |
amod |
human,libraries |
R1440 |
T2424 |
T2420 |
acomp |
available,were |
R1441 |
T2425 |
T2411 |
punct |
.,chose |
R1442 |
T2427 |
T2428 |
prep |
Given,reasoned |
R1443 |
T2429 |
T2430 |
det |
the,fact |
R1444 |
T2430 |
T2427 |
pobj |
fact,Given |
R1445 |
T2431 |
T2432 |
mark |
that,is |
R1446 |
T2432 |
T2430 |
acl |
is,fact |
R1447 |
T2433 |
T2434 |
det |
the,sequence |
R1448 |
T2434 |
T2432 |
nsubj |
sequence,is |
R1449 |
T2435 |
T2436 |
compound |
amino,acid |
R1450 |
T2436 |
T2434 |
compound |
acid,sequence |
R1451 |
T2437 |
T2434 |
prep |
of,sequence |
R1452 |
T2438 |
T2439 |
det |
the,protein |
R1453 |
T2439 |
T2437 |
pobj |
protein,of |
R1454 |
T2440 |
T2439 |
compound |
PAX6,protein |
R1455 |
T2441 |
T2432 |
acomp |
identical,is |
R1456 |
T2442 |
T2432 |
prep |
in,is |
R1457 |
T2443 |
T2442 |
pobj |
man,in |
R1458 |
T2444 |
T2443 |
cc |
and,man |
R1459 |
T2445 |
T2443 |
conj |
mouse,man |
R1460 |
T2446 |
T2428 |
punct |
", ",reasoned |
R1461 |
T2447 |
T2428 |
nsubj |
we,reasoned |
R1462 |
T2448 |
T2449 |
mark |
that,yield |
R1463 |
T2449 |
T2428 |
ccomp |
yield,reasoned |
R1464 |
T2450 |
T2451 |
det |
a,library |
R1465 |
T2451 |
T2449 |
nsubj |
library,yield |
R1466 |
T2452 |
T2453 |
compound |
mouse,brain |
R1467 |
T2453 |
T2451 |
compound |
brain,library |
R1468 |
T2454 |
T2449 |
aux |
would,yield |
R1469 |
T2455 |
T2456 |
amod |
relevant,interactors |
R1470 |
T2456 |
T2449 |
dobj |
interactors,yield |
R1471 |
T2457 |
T2428 |
punct |
.,reasoned |
R1472 |
T2459 |
T2460 |
prep |
For,carried |
R1473 |
T2461 |
T2459 |
pobj |
comparison,For |
R1474 |
T2462 |
T2460 |
nsubj |
we,carried |
R1475 |
T2463 |
T2460 |
advmod |
also,carried |
R1476 |
T2464 |
T2460 |
prt |
out,carried |
R1477 |
T2465 |
T2466 |
det |
the,screen |
R1478 |
T2466 |
T2460 |
dobj |
screen,carried |
R1479 |
T2467 |
T2460 |
advcl |
using,carried |
R1480 |
T2468 |
T2469 |
det |
a,construct |
R1481 |
T2469 |
T2467 |
dobj |
construct,using |
R1482 |
T2470 |
T2469 |
acl |
containing,construct |
R1483 |
T2471 |
T2472 |
det |
the,domain |
R1484 |
T2472 |
T2470 |
dobj |
domain,containing |
R1485 |
T2473 |
T2472 |
amod |
whole,domain |
R1486 |
T2474 |
T2472 |
compound |
PST,domain |
R1487 |
T2475 |
T2469 |
punct |
(,construct |
R1488 |
T2476 |
T2469 |
appos |
PAX6PST,construct |
R1489 |
T2477 |
T2469 |
punct |
),construct |
R1490 |
T2478 |
T2460 |
punct |
.,carried |