PMC:1208879 / 5685-17652 JSON TXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T1765	9-10	DT	denotes	A
T1767	11-17	RB	denotes	highly
T1768	18-27	JJ	denotes	conserved
T1769	28-29	NN	denotes	C
T1771	29-30	HYPH	denotes	-
T1770	30-38	JJ	denotes	terminal
T1772	39-43	NN	denotes	PAX6
T1766	44-51	NN	denotes	peptide
T1773	51-170	sentence	denotes	We and others [31-33] noted that there is significant sequence conservation at the C-terminal end of the PAX6 protein.
T1774	52-54	PRP	denotes	We
T1776	55-58	CC	denotes	and
T1777	59-65	NNS	denotes	others
T1778	66-67	-LRB-	denotes	[
T1779	67-69	CD	denotes	31
T1780	69-70	SYM	denotes	-
T1781	70-72	CD	denotes	33
T1782	72-73	-RRB-	denotes	]
T1775	74-79	VBD	denotes	noted
T1783	80-84	IN	denotes	that
T1785	85-90	EX	denotes	there
T1784	91-93	VBZ	denotes	is
T1786	94-105	JJ	denotes	significant
T1788	106-114	NN	denotes	sequence
T1787	115-127	NN	denotes	conservation
T1789	128-130	IN	denotes	at
T1790	131-134	DT	denotes	the
T1792	135-136	NN	denotes	C
T1794	136-137	HYPH	denotes	-
T1793	137-145	JJ	denotes	terminal
T1791	146-149	NN	denotes	end
T1795	150-152	IN	denotes	of
T1796	153-156	DT	denotes	the
T1798	157-161	NN	denotes	PAX6
T1797	162-169	NN	denotes	protein
T1799	169-170	.	denotes	.
T1800	170-358	sentence	denotes	BLAST analysis of the amino acid sequence of the PAX6 PST domain (aa 278–422) revealed a highly conserved motif within the last 28 amino acids (beginning at the 'GLISP' motif, Figure 1a).
T1801	171-176	NN	denotes	BLAST
T1802	177-185	NN	denotes	analysis
T1804	186-188	IN	denotes	of
T1805	189-192	DT	denotes	the
T1807	193-198	NN	denotes	amino
T1808	199-203	NN	denotes	acid
T1806	204-212	NN	denotes	sequence
T1809	213-215	IN	denotes	of
T1810	216-219	DT	denotes	the
T1812	220-224	NN	denotes	PAX6
T1813	225-228	NN	denotes	PST
T1811	229-235	NN	denotes	domain
T1814	236-237	-LRB-	denotes	(
T1816	237-239	NN	denotes	aa
T1815	240-243	CD	denotes	278
T1817	243-244	SYM	denotes	–
T1818	244-247	CD	denotes	422
T1819	247-248	-RRB-	denotes	)
T1803	249-257	VBD	denotes	revealed
T1820	258-259	DT	denotes	a
T1822	260-266	RB	denotes	highly
T1823	267-276	JJ	denotes	conserved
T1821	277-282	NN	denotes	motif
T1824	283-289	IN	denotes	within
T1825	290-293	DT	denotes	the
T1827	294-298	JJ	denotes	last
T1828	299-301	CD	denotes	28
T1829	302-307	NN	denotes	amino
T1826	308-313	NNS	denotes	acids
T1830	314-315	-LRB-	denotes	(
T1831	315-324	VBG	denotes	beginning
T1832	325-327	IN	denotes	at
T1833	328-331	DT	denotes	the
T1835	332-333	``	denotes	'
T1836	333-338	NN	denotes	GLISP
T1837	338-339	''	denotes	'
T1834	340-345	NN	denotes	motif
T1838	345-347	,	denotes	,
T1839	347-353	NN	denotes	Figure
T1840	354-356	CD	denotes	1a
T1841	356-357	-RRB-	denotes	)
T1842	357-358	.	denotes	.
T1843	358-506	sentence	denotes	Strong conservation was seen in distantly related species such as axolotl (Ambystoma mexicanum) and sea urchin (Paracentrotus lividus) (Figure 1a).
T1844	359-365	JJ	denotes	Strong
T1845	366-378	NN	denotes	conservation
T1847	379-382	VBD	denotes	was
T1846	383-387	VBN	denotes	seen
T1848	388-390	IN	denotes	in
T1849	391-400	RB	denotes	distantly
T1850	401-408	VBN	denotes	related
T1851	409-416	NNS	denotes	species
T1852	417-421	JJ	denotes	such
T1853	422-424	IN	denotes	as
T1854	425-432	NN	denotes	axolotl
T1855	433-434	-LRB-	denotes	(
T1856	434-443	NNP	denotes	Ambystoma
T1857	444-453	NNP	denotes	mexicanum
T1858	453-454	-RRB-	denotes	)
T1859	455-458	CC	denotes	and
T1860	459-462	NN	denotes	sea
T1861	463-469	NN	denotes	urchin
T1862	470-471	-LRB-	denotes	(
T1863	471-484	NNP	denotes	Paracentrotus
T1864	485-492	NNP	denotes	lividus
T1865	492-493	-RRB-	denotes	)
T1866	494-495	-LRB-	denotes	(
T1867	495-501	NN	denotes	Figure
T1868	502-504	CD	denotes	1a
T1869	504-505	-RRB-	denotes	)
T1870	505-506	.	denotes	.
T1871	506-1239	sentence	denotes	Figure 1 Characterisation of the PAX6 C-terminal peptide. (a) CLUSTAL alignment of the terminal 42 amino acids of PAX6 from diverse species. (*) indicates invariant residues, (:) indicates highly similar substitutions and (.) indicates moderately similar substitutions. (b) Secondary structure analysis of the highly conserved terminal 28 amino acids predicts a single beta sheet (arrow) in the SVPVQ peptide. (c) When 4 residues are added in the N-terminal direction, 2 beta sheets are now predicted. We subjected the whole PAX6 PST domain to secondary structure analysis using JPRED, a program that uses a number of different protein structure prediction algorithms to generate a consensus secondary structure (Figure 1) [25,26].
T8299	517-533	NN	denotes	Characterisation
T8300	534-536	IN	denotes	of
T8301	537-540	DT	denotes	the
T8303	541-545	NN	denotes	PAX6
T8304	546-547	NN	denotes	C
T8306	547-548	HYPH	denotes	-
T8305	548-556	JJ	denotes	terminal
T8302	557-564	NN	denotes	peptide
T8307	564-565	.	denotes	.
T8308	565-648	sentence	denotes	(a) CLUSTAL alignment of the terminal 42 amino acids of PAX6 from diverse species.
T8309	566-567	-LRB-	denotes	(
T8310	567-568	LS	denotes	a
T8312	568-569	-RRB-	denotes	)
T8313	570-577	NNP	denotes	CLUSTAL
T8311	578-587	NN	denotes	alignment
T8314	588-590	IN	denotes	of
T8315	591-594	DT	denotes	the
T8317	595-603	JJ	denotes	terminal
T8318	604-606	CD	denotes	42
T8319	607-612	NN	denotes	amino
T8316	613-618	NNS	denotes	acids
T8320	619-621	IN	denotes	of
T8321	622-626	NN	denotes	PAX6
T8322	627-631	IN	denotes	from
T8323	632-639	JJ	denotes	diverse
T8324	640-647	NNS	denotes	species
T8325	647-648	.	denotes	.
T8326	648-777	sentence	denotes	(*) indicates invariant residues, (:) indicates highly similar substitutions and (.) indicates moderately similar substitutions.
T8327	649-650	-LRB-	denotes	(
T8329	650-651	SYM	denotes	*
T8330	651-652	-RRB-	denotes	)
T8328	653-662	VBZ	denotes	indicates
T8332	663-672	JJ	denotes	invariant
T8333	673-681	NNS	denotes	residues
T8334	681-683	,	denotes	,
T8335	683-684	-LRB-	denotes	(
T8336	684-685	SYM	denotes	:
T8337	685-686	-RRB-	denotes	)
T8331	687-696	VBZ	denotes	indicates
T8338	697-703	RB	denotes	highly
T8339	704-711	JJ	denotes	similar
T8340	712-725	NNS	denotes	substitutions
T8341	726-729	CC	denotes	and
T8342	730-731	-LRB-	denotes	(
T8344	731-732	SYM	denotes	.
T8345	732-733	-RRB-	denotes	)
T8343	734-743	VBZ	denotes	indicates
T8346	744-754	RB	denotes	moderately
T8347	755-762	JJ	denotes	similar
T8348	763-776	NNS	denotes	substitutions
T8349	776-777	.	denotes	.
T8350	777-917	sentence	denotes	(b) Secondary structure analysis of the highly conserved terminal 28 amino acids predicts a single beta sheet (arrow) in the SVPVQ peptide.
T8351	778-779	-LRB-	denotes	(
T8352	779-780	LS	denotes	b
T8354	780-781	-RRB-	denotes	)
T8355	782-791	JJ	denotes	Secondary
T8357	792-801	NN	denotes	structure
T8356	802-810	NN	denotes	analysis
T8358	811-813	IN	denotes	of
T8359	814-817	DT	denotes	the
T8361	818-824	RB	denotes	highly
T8362	825-834	JJ	denotes	conserved
T8363	835-843	JJ	denotes	terminal
T8364	844-846	CD	denotes	28
T8365	847-852	NN	denotes	amino
T8360	853-858	NNS	denotes	acids
T8353	859-867	VBZ	denotes	predicts
T8366	868-869	DT	denotes	a
T8368	870-876	JJ	denotes	single
T8369	877-881	NN	denotes	beta
T8367	882-887	NN	denotes	sheet
T8370	888-889	-LRB-	denotes	(
T8371	889-894	NN	denotes	arrow
T8372	894-895	-RRB-	denotes	)
T8373	896-898	IN	denotes	in
T8374	899-902	DT	denotes	the
T8376	903-908	NN	denotes	SVPVQ
T8375	909-916	NN	denotes	peptide
T8377	916-917	.	denotes	.
T8378	917-1009	sentence	denotes	(c) When 4 residues are added in the N-terminal direction, 2 beta sheets are now predicted.
T8379	918-919	-LRB-	denotes	(
T8380	919-920	LS	denotes	c
T8382	920-921	-RRB-	denotes	)
T8383	922-926	WRB	denotes	When
T8385	927-928	CD	denotes	4
T8386	929-937	NNS	denotes	residues
T8387	938-941	VBP	denotes	are
T8384	942-947	VBN	denotes	added
T8388	948-950	IN	denotes	in
T8389	951-954	DT	denotes	the
T8391	955-956	NN	denotes	N
T8393	956-957	HYPH	denotes	-
T8392	957-965	JJ	denotes	terminal
T8390	966-975	NN	denotes	direction
T8394	975-977	,	denotes	,
T8395	977-978	CD	denotes	2
T8397	979-983	NN	denotes	beta
T8396	984-990	NNS	denotes	sheets
T8398	991-994	VBP	denotes	are
T8399	995-998	RB	denotes	now
T8381	999-1008	VBN	denotes	predicted
T8400	1008-1009	.	denotes	.
T1872	1010-1012	PRP	denotes	We
T1873	1013-1022	VBD	denotes	subjected
T1874	1023-1026	DT	denotes	the
T1876	1027-1032	JJ	denotes	whole
T1877	1033-1037	NN	denotes	PAX6
T1878	1038-1041	NN	denotes	PST
T1875	1042-1048	NN	denotes	domain
T1879	1049-1051	IN	denotes	to
T1880	1052-1061	JJ	denotes	secondary
T1882	1062-1071	NN	denotes	structure
T1881	1072-1080	NN	denotes	analysis
T1883	1081-1086	VBG	denotes	using
T1884	1087-1092	NNP	denotes	JPRED
T1885	1092-1094	,	denotes	,
T1886	1094-1095	DT	denotes	a
T1887	1096-1103	NN	denotes	program
T1888	1104-1108	WDT	denotes	that
T1889	1109-1113	VBZ	denotes	uses
T1890	1114-1115	DT	denotes	a
T1891	1116-1122	NN	denotes	number
T1892	1123-1125	IN	denotes	of
T1893	1126-1135	JJ	denotes	different
T1895	1136-1143	NN	denotes	protein
T1896	1144-1153	NN	denotes	structure
T1897	1154-1164	NN	denotes	prediction
T1894	1165-1175	NNS	denotes	algorithms
T1898	1176-1178	TO	denotes	to
T1899	1179-1187	VB	denotes	generate
T1900	1188-1189	DT	denotes	a
T1902	1190-1199	NN	denotes	consensus
T1903	1200-1209	JJ	denotes	secondary
T1901	1210-1219	NN	denotes	structure
T1904	1220-1221	-LRB-	denotes	(
T1905	1221-1227	NN	denotes	Figure
T1906	1228-1229	CD	denotes	1
T1907	1229-1230	-RRB-	denotes	)
T1908	1231-1232	-LRB-	denotes	[
T1910	1232-1234	CD	denotes	25
T1911	1234-1235	,	denotes	,
T1909	1235-1237	CD	denotes	26
T1912	1237-1238	-RRB-	denotes	]
T1913	1238-1239	.	denotes	.
T1914	1239-1519	sentence	denotes	The PST domain was largely devoid of predicted secondary structure except for the C-terminal region, which contained two predicted beta sheets within the highly conserved domain, one in the 'GLISP' motif and one in the 'SPVPQ' motif (identical to the pattern shown in Figure 1c).
T1915	1240-1243	DT	denotes	The
T1917	1244-1247	NN	denotes	PST
T1916	1248-1254	NN	denotes	domain
T1918	1255-1258	VBD	denotes	was
T1919	1259-1266	RB	denotes	largely
T1920	1267-1273	JJ	denotes	devoid
T1921	1274-1276	IN	denotes	of
T1922	1277-1286	VBN	denotes	predicted
T1924	1287-1296	JJ	denotes	secondary
T1923	1297-1306	NN	denotes	structure
T1925	1307-1313	IN	denotes	except
T1926	1314-1317	IN	denotes	for
T1927	1318-1321	DT	denotes	the
T1929	1322-1323	NN	denotes	C
T1931	1323-1324	HYPH	denotes	-
T1930	1324-1332	JJ	denotes	terminal
T1928	1333-1339	NN	denotes	region
T1932	1339-1341	,	denotes	,
T1933	1341-1346	WDT	denotes	which
T1934	1347-1356	VBD	denotes	contained
T1935	1357-1360	CD	denotes	two
T1937	1361-1370	VBN	denotes	predicted
T1938	1371-1375	NN	denotes	beta
T1936	1376-1382	NNS	denotes	sheets
T1939	1383-1389	IN	denotes	within
T1940	1390-1393	DT	denotes	the
T1942	1394-1400	RB	denotes	highly
T1943	1401-1410	JJ	denotes	conserved
T1941	1411-1417	NN	denotes	domain
T1944	1417-1419	,	denotes	,
T1945	1419-1422	CD	denotes	one
T1946	1423-1425	IN	denotes	in
T1947	1426-1429	DT	denotes	the
T1949	1430-1431	``	denotes	'
T1948	1431-1436	NN	denotes	GLISP
T1951	1436-1437	''	denotes	'
T1950	1438-1443	NN	denotes	motif
T1952	1444-1447	CC	denotes	and
T1953	1448-1451	CD	denotes	one
T1954	1452-1454	IN	denotes	in
T1955	1455-1458	DT	denotes	the
T1957	1459-1460	``	denotes	'
T1956	1460-1465	NN	denotes	SPVPQ
T1959	1465-1466	''	denotes	'
T1958	1467-1472	NN	denotes	motif
T1960	1473-1474	-LRB-	denotes	(
T1961	1474-1483	JJ	denotes	identical
T1962	1484-1486	IN	denotes	to
T1963	1487-1490	DT	denotes	the
T1964	1491-1498	NN	denotes	pattern
T1965	1499-1504	VBN	denotes	shown
T1966	1505-1507	IN	denotes	in
T1967	1508-1514	NN	denotes	Figure
T1968	1515-1517	CD	denotes	1c
T1969	1517-1518	-RRB-	denotes	)
T1970	1518-1519	.	denotes	.
T1971	1519-1725	sentence	denotes	Initially we defined the C-terminal domain as running from the 'GLISP' motif up to the stop codon, since this region was most highly conserved and contained strongly predicted secondary structure elements.
T1972	1520-1529	RB	denotes	Initially
T1974	1530-1532	PRP	denotes	we
T1973	1533-1540	VBD	denotes	defined
T1975	1541-1544	DT	denotes	the
T1977	1545-1546	NN	denotes	C
T1979	1546-1547	HYPH	denotes	-
T1978	1547-1555	JJ	denotes	terminal
T1976	1556-1562	NN	denotes	domain
T1980	1563-1565	IN	denotes	as
T1981	1566-1573	VBG	denotes	running
T1982	1574-1578	IN	denotes	from
T1983	1579-1582	DT	denotes	the
T1985	1583-1584	``	denotes	'
T1984	1584-1589	NN	denotes	GLISP
T1987	1589-1590	''	denotes	'
T1986	1591-1596	NN	denotes	motif
T1988	1597-1599	IN	denotes	up
T1989	1600-1602	IN	denotes	to
T1990	1603-1606	DT	denotes	the
T1992	1607-1611	NN	denotes	stop
T1991	1612-1617	NN	denotes	codon
T1993	1617-1619	,	denotes	,
T1994	1619-1624	IN	denotes	since
T1996	1625-1629	DT	denotes	this
T1997	1630-1636	NN	denotes	region
T1998	1637-1640	VBD	denotes	was
T1999	1641-1645	RBS	denotes	most
T2000	1646-1652	RB	denotes	highly
T1995	1653-1662	VBN	denotes	conserved
T2001	1663-1666	CC	denotes	and
T2002	1667-1676	VBD	denotes	contained
T2003	1677-1685	RB	denotes	strongly
T2004	1686-1695	VBN	denotes	predicted
T2006	1696-1705	JJ	denotes	secondary
T2007	1706-1715	NN	denotes	structure
T2005	1716-1724	NNS	denotes	elements
T2008	1724-1725	.	denotes	.
T2009	1725-1865	sentence	denotes	However when we performed secondary structure prediction analysis on this 28 amino acid peptide, the first beta sheet was lost (Figure 1b).
T2010	1726-1733	RB	denotes	However
T2012	1734-1738	WRB	denotes	when
T2014	1739-1741	PRP	denotes	we
T2013	1742-1751	VBD	denotes	performed
T2015	1752-1761	JJ	denotes	secondary
T2016	1762-1771	NN	denotes	structure
T2017	1772-1782	NN	denotes	prediction
T2018	1783-1791	NN	denotes	analysis
T2019	1792-1794	IN	denotes	on
T2020	1795-1799	DT	denotes	this
T2022	1800-1802	CD	denotes	28
T2024	1803-1808	NN	denotes	amino
T2023	1809-1813	NN	denotes	acid
T2021	1814-1821	NN	denotes	peptide
T2025	1821-1823	,	denotes	,
T2026	1823-1826	DT	denotes	the
T2028	1827-1832	JJ	denotes	first
T2029	1833-1837	NN	denotes	beta
T2027	1838-1843	NN	denotes	sheet
T2030	1844-1847	VBD	denotes	was
T2011	1848-1852	VBN	denotes	lost
T2031	1853-1854	-LRB-	denotes	(
T2032	1854-1860	NN	denotes	Figure
T2033	1861-1863	CD	denotes	1b
T2034	1863-1864	-RRB-	denotes	)
T2035	1864-1865	.	denotes	.
T2036	1865-1986	sentence	denotes	Addition of another 4 amino acids (TTST) immediately before 'GLISP' caused recovery of the first beta sheet (Figure 1c).
T2037	1866-1874	NN	denotes	Addition
T2039	1875-1877	IN	denotes	of
T2040	1878-1885	DT	denotes	another
T2042	1886-1887	CD	denotes	4
T2043	1888-1893	NN	denotes	amino
T2041	1894-1899	NNS	denotes	acids
T2044	1900-1901	-LRB-	denotes	(
T2045	1901-1905	NN	denotes	TTST
T2046	1905-1906	-RRB-	denotes	)
T2047	1907-1918	RB	denotes	immediately
T2048	1919-1925	IN	denotes	before
T2049	1926-1927	``	denotes	'
T2050	1927-1932	NN	denotes	GLISP
T2051	1932-1933	''	denotes	'
T2038	1934-1940	VBN	denotes	caused
T2052	1941-1949	NN	denotes	recovery
T2053	1950-1952	IN	denotes	of
T2054	1953-1956	DT	denotes	the
T2056	1957-1962	JJ	denotes	first
T2057	1963-1967	NN	denotes	beta
T2055	1968-1973	NN	denotes	sheet
T2058	1974-1975	-LRB-	denotes	(
T2059	1975-1981	NN	denotes	Figure
T2060	1982-1984	CD	denotes	1c
T2061	1984-1985	-RRB-	denotes	)
T2062	1985-1986	.	denotes	.
T2063	1986-2150	sentence	denotes	Although these 4 residues are not highly conserved (Figure 1a), they appear to be important for seeding the first beta sheet and therefore for secondary structure.
T2064	1987-1995	IN	denotes	Although
T2066	1996-2001	DT	denotes	these
T2068	2002-2003	CD	denotes	4
T2067	2004-2012	NNS	denotes	residues
T2065	2013-2016	VBP	denotes	are
T2070	2017-2020	RB	denotes	not
T2071	2021-2027	RB	denotes	highly
T2072	2028-2037	VBN	denotes	conserved
T2073	2038-2039	-LRB-	denotes	(
T2074	2039-2045	NN	denotes	Figure
T2075	2046-2048	CD	denotes	1a
T2076	2048-2049	-RRB-	denotes	)
T2077	2049-2051	,	denotes	,
T2078	2051-2055	PRP	denotes	they
T2069	2056-2062	VBP	denotes	appear
T2079	2063-2065	TO	denotes	to
T2080	2066-2068	VB	denotes	be
T2081	2069-2078	JJ	denotes	important
T2082	2079-2082	IN	denotes	for
T2083	2083-2090	VBG	denotes	seeding
T2084	2091-2094	DT	denotes	the
T2086	2095-2100	JJ	denotes	first
T2087	2101-2105	NN	denotes	beta
T2085	2106-2111	NN	denotes	sheet
T2088	2112-2115	CC	denotes	and
T2089	2116-2125	RB	denotes	therefore
T2090	2126-2129	IN	denotes	for
T2091	2130-2139	JJ	denotes	secondary
T2092	2140-2149	NN	denotes	structure
T2093	2149-2150	.	denotes	.
T2094	2150-2301	sentence	denotes	Thus we define the C-terminal peptide as being the final 32 amino acids of PAX6, running from threonine 391 to the stop codon (top line of Figure 1c).
T2095	2151-2155	RB	denotes	Thus
T2097	2156-2158	PRP	denotes	we
T2096	2159-2165	VBP	denotes	define
T2098	2166-2169	DT	denotes	the
T2100	2170-2171	NN	denotes	C
T2102	2171-2172	HYPH	denotes	-
T2101	2172-2180	JJ	denotes	terminal
T2099	2181-2188	NN	denotes	peptide
T2103	2189-2191	IN	denotes	as
T2104	2192-2197	VBG	denotes	being
T2105	2198-2201	DT	denotes	the
T2107	2202-2207	JJ	denotes	final
T2108	2208-2210	CD	denotes	32
T2109	2211-2216	NN	denotes	amino
T2106	2217-2222	NNS	denotes	acids
T2110	2223-2225	IN	denotes	of
T2111	2226-2230	NN	denotes	PAX6
T2112	2230-2232	,	denotes	,
T2113	2232-2239	VBG	denotes	running
T2114	2240-2244	IN	denotes	from
T2115	2245-2254	NN	denotes	threonine
T2116	2255-2258	CD	denotes	391
T2117	2259-2261	IN	denotes	to
T2118	2262-2265	DT	denotes	the
T2120	2266-2270	NN	denotes	stop
T2119	2271-2276	NN	denotes	codon
T2121	2277-2278	-LRB-	denotes	(
T2123	2278-2281	NN	denotes	top
T2122	2282-2286	NN	denotes	line
T2124	2287-2289	IN	denotes	of
T2125	2290-2296	NN	denotes	Figure
T2126	2297-2299	CD	denotes	1c
T2127	2299-2300	-RRB-	denotes	)
T2128	2300-2301	.	denotes	.
T2344	2303-2308	NN	denotes	Yeast
T2346	2309-2312	CD	denotes	two
T2348	2312-2313	HYPH	denotes	-
T2347	2313-2319	NN	denotes	hybrid
T2345	2320-2329	NN	denotes	screening
T2349	2329-2626	sentence	denotes	We hypothesised that the C-terminal peptide might be involved in protein-protein interactions, and we tested this by screening a cDNA library using the yeast two-hybrid system with a construct (PAX6CTP) in which the 32 amino acid C-terminal peptide was fused to the yeast GAL4 DNA binding domain.
T2350	2330-2332	PRP	denotes	We
T2351	2333-2345	VBD	denotes	hypothesised
T2352	2346-2350	IN	denotes	that
T2354	2351-2354	DT	denotes	the
T2356	2355-2356	NN	denotes	C
T2358	2356-2357	HYPH	denotes	-
T2357	2357-2365	JJ	denotes	terminal
T2355	2366-2373	NN	denotes	peptide
T2359	2374-2379	MD	denotes	might
T2360	2380-2382	VB	denotes	be
T2353	2383-2391	VBN	denotes	involved
T2361	2392-2394	IN	denotes	in
T2362	2395-2402	NN	denotes	protein
T2364	2402-2403	SYM	denotes	-
T2363	2403-2410	NN	denotes	protein
T2365	2411-2423	NNS	denotes	interactions
T2366	2423-2425	,	denotes	,
T2367	2425-2428	CC	denotes	and
T2368	2429-2431	PRP	denotes	we
T2369	2432-2438	VBD	denotes	tested
T2370	2439-2443	DT	denotes	this
T2371	2444-2446	IN	denotes	by
T2372	2447-2456	VBG	denotes	screening
T2373	2457-2458	DT	denotes	a
T2375	2459-2463	NN	denotes	cDNA
T2374	2464-2471	NN	denotes	library
T2376	2472-2477	VBG	denotes	using
T2377	2478-2481	DT	denotes	the
T2379	2482-2487	NN	denotes	yeast
T2380	2488-2491	CD	denotes	two
T2382	2491-2492	HYPH	denotes	-
T2381	2492-2498	NN	denotes	hybrid
T2378	2499-2505	NN	denotes	system
T2383	2506-2510	IN	denotes	with
T2384	2511-2512	DT	denotes	a
T2385	2513-2522	NN	denotes	construct
T2386	2523-2524	-LRB-	denotes	(
T2387	2524-2531	NN	denotes	PAX6CTP
T2388	2531-2532	-RRB-	denotes	)
T2389	2533-2535	IN	denotes	in
T2391	2536-2541	WDT	denotes	which
T2392	2542-2545	DT	denotes	the
T2394	2546-2548	CD	denotes	32
T2396	2549-2554	NN	denotes	amino
T2395	2555-2559	NN	denotes	acid
T2397	2560-2561	NN	denotes	C
T2399	2561-2562	HYPH	denotes	-
T2398	2562-2570	JJ	denotes	terminal
T2393	2571-2578	NN	denotes	peptide
T2400	2579-2582	VBD	denotes	was
T2390	2583-2588	VBN	denotes	fused
T2401	2589-2591	IN	denotes	to
T2402	2592-2595	DT	denotes	the
T2404	2596-2601	NN	denotes	yeast
T2405	2602-2606	NN	denotes	GAL4
T2406	2607-2610	NN	denotes	DNA
T2407	2611-2618	NN	denotes	binding
T2403	2619-2625	NN	denotes	domain
T2408	2625-2626	.	denotes	.
T2409	2626-2710	sentence	denotes	We chose to screen a mouse brain cDNA library as no human libraries were available.
T2410	2627-2629	PRP	denotes	We
T2411	2630-2635	VBD	denotes	chose
T2412	2636-2638	TO	denotes	to
T2413	2639-2645	VB	denotes	screen
T2414	2646-2647	DT	denotes	a
T2416	2648-2653	NN	denotes	mouse
T2417	2654-2659	NN	denotes	brain
T2418	2660-2664	NN	denotes	cDNA
T2415	2665-2672	NN	denotes	library
T2419	2673-2675	IN	denotes	as
T2421	2676-2678	DT	denotes	no
T2423	2679-2684	JJ	denotes	human
T2422	2685-2694	NNS	denotes	libraries
T2420	2695-2699	VBD	denotes	were
T2424	2700-2709	JJ	denotes	available
T2425	2709-2710	.	denotes	.
T2426	2710-2878	sentence	denotes	Given the fact that the amino acid sequence of the PAX6 protein is identical in man and mouse, we reasoned that a mouse brain library would yield relevant interactors.
T2427	2711-2716	VBN	denotes	Given
T2429	2717-2720	DT	denotes	the
T2430	2721-2725	NN	denotes	fact
T2431	2726-2730	IN	denotes	that
T2433	2731-2734	DT	denotes	the
T2435	2735-2740	NN	denotes	amino
T2436	2741-2745	NN	denotes	acid
T2434	2746-2754	NN	denotes	sequence
T2437	2755-2757	IN	denotes	of
T2438	2758-2761	DT	denotes	the
T2440	2762-2766	NN	denotes	PAX6
T2439	2767-2774	NN	denotes	protein
T2432	2775-2777	VBZ	denotes	is
T2441	2778-2787	JJ	denotes	identical
T2442	2788-2790	IN	denotes	in
T2443	2791-2794	NN	denotes	man
T2444	2795-2798	CC	denotes	and
T2445	2799-2804	NN	denotes	mouse
T2446	2804-2806	,	denotes	,
T2447	2806-2808	PRP	denotes	we
T2428	2809-2817	VBD	denotes	reasoned
T2448	2818-2822	IN	denotes	that
T2450	2823-2824	DT	denotes	a
T2452	2825-2830	NN	denotes	mouse
T2453	2831-2836	NN	denotes	brain
T2451	2837-2844	NN	denotes	library
T2454	2845-2850	MD	denotes	would
T2449	2851-2856	VB	denotes	yield
T2455	2857-2865	JJ	denotes	relevant
T2456	2866-2877	NNS	denotes	interactors
T2457	2877-2878	.	denotes	.
T2458	2878-2985	sentence	denotes	For comparison we also carried out the screen using a construct containing the whole PST domain (PAX6PST).
T2459	2879-2882	IN	denotes	For
T2461	2883-2893	NN	denotes	comparison
T2462	2894-2896	PRP	denotes	we
T2463	2897-2901	RB	denotes	also
T2460	2902-2909	VBD	denotes	carried
T2464	2910-2913	RP	denotes	out
T2465	2914-2917	DT	denotes	the
T2466	2918-2924	NN	denotes	screen
T2467	2925-2930	VBG	denotes	using
T2468	2931-2932	DT	denotes	a
T2469	2933-2942	NN	denotes	construct
T2470	2943-2953	VBG	denotes	containing
T2471	2954-2957	DT	denotes	the
T2473	2958-2963	JJ	denotes	whole
T2474	2964-2967	NN	denotes	PST
T2472	2968-2974	NN	denotes	domain
T2475	2975-2976	-LRB-	denotes	(
T2476	2976-2983	NN	denotes	PAX6PST
T2477	2983-2984	-RRB-	denotes	)
T2478	2984-2985	.	denotes	.
T2479	2985-3120	sentence	denotes	The C-terminal peptide screen gave 15 colonies that were positive with all three reporters and the PST domain screen gave 62 colonies.
T2480	2986-2989	DT	denotes	The
T2482	2990-2991	NN	denotes	C
T2484	2991-2992	HYPH	denotes	-
T2483	2992-3000	JJ	denotes	terminal
T2485	3001-3008	NN	denotes	peptide
T2481	3009-3015	NN	denotes	screen
T2486	3016-3020	VBD	denotes	gave
T2487	3021-3023	CD	denotes	15
T2488	3024-3032	NNS	denotes	colonies
T2489	3033-3037	WDT	denotes	that
T2490	3038-3042	VBD	denotes	were
T2491	3043-3051	JJ	denotes	positive
T2492	3052-3056	IN	denotes	with
T2493	3057-3060	DT	denotes	all
T2495	3061-3066	CD	denotes	three
T2494	3067-3076	NNS	denotes	reporters
T2496	3077-3080	CC	denotes	and
T2497	3081-3084	DT	denotes	the
T2499	3085-3088	NN	denotes	PST
T2500	3089-3095	NN	denotes	domain
T2498	3096-3102	NN	denotes	screen
T2501	3103-3107	VBD	denotes	gave
T2502	3108-3110	CD	denotes	62
T2503	3111-3119	NNS	denotes	colonies
T2504	3119-3120	.	denotes	.
T2505	3120-3191	sentence	denotes	The interacting plasmids were isolated and the cDNA inserts sequenced.
T2506	3121-3124	DT	denotes	The
T2508	3125-3136	VBG	denotes	interacting
T2507	3137-3145	NNS	denotes	plasmids
T2510	3146-3150	VBD	denotes	were
T2509	3151-3159	VBN	denotes	isolated
T2511	3160-3163	CC	denotes	and
T2512	3164-3167	DT	denotes	the
T2514	3168-3172	NN	denotes	cDNA
T2513	3173-3180	NNS	denotes	inserts
T2515	3181-3190	VBN	denotes	sequenced
T2516	3190-3191	.	denotes	.
T2517	3191-3402	sentence	denotes	Three cDNAs were identified 3 or more times, Homer3 (NM_011984), Dncl1 (Dynein cytoplasmic light chain 1, NM_019682, also known as Pin or Dlc8) and Trim11 (Tripartite motif protein family member 11, NM_053168).
T2518	3192-3197	CD	denotes	Three
T2519	3198-3203	NNS	denotes	cDNAs
T2521	3204-3208	VBD	denotes	were
T2520	3209-3219	VBN	denotes	identified
T2522	3220-3221	CD	denotes	3
T2524	3222-3224	CC	denotes	or
T2525	3225-3229	JJR	denotes	more
T2523	3230-3235	NNS	denotes	times
T2526	3235-3237	,	denotes	,
T2527	3237-3243	NN	denotes	Homer3
T2528	3244-3245	-LRB-	denotes	(
T2529	3245-3254	NN	denotes	NM_011984
T2530	3254-3255	-RRB-	denotes	)
T2531	3255-3257	,	denotes	,
T2532	3257-3262	NN	denotes	Dncl1
T2533	3263-3264	-LRB-	denotes	(
T2534	3264-3270	NNP	denotes	Dynein
T2536	3271-3282	JJ	denotes	cytoplasmic
T2537	3283-3288	JJ	denotes	light
T2535	3289-3294	NN	denotes	chain
T2538	3295-3296	CD	denotes	1
T2539	3296-3298	,	denotes	,
T2540	3298-3307	NN	denotes	NM_019682
T2541	3307-3309	,	denotes	,
T2542	3309-3313	RB	denotes	also
T2543	3314-3319	VBN	denotes	known
T2544	3320-3322	IN	denotes	as
T2545	3323-3326	NN	denotes	Pin
T2546	3327-3329	CC	denotes	or
T2547	3330-3334	NN	denotes	Dlc8
T2548	3334-3335	-RRB-	denotes	)
T2549	3336-3339	CC	denotes	and
T2550	3340-3346	NN	denotes	Trim11
T2551	3347-3348	-LRB-	denotes	(
T2552	3348-3358	JJ	denotes	Tripartite
T2553	3359-3364	NN	denotes	motif
T2555	3365-3372	NN	denotes	protein
T2556	3373-3379	NN	denotes	family
T2554	3380-3386	NN	denotes	member
T2557	3387-3389	CD	denotes	11
T2558	3389-3391	,	denotes	,
T2559	3391-3400	NN	denotes	NM_053168
T2560	3400-3401	-RRB-	denotes	)
T2561	3401-3402	.	denotes	.
T2562	3402-3491	sentence	denotes	Homer3 (6 clones) and Dncl1 (2 clones) were identified in the C-terminal peptide screen.
T2563	3403-3409	NN	denotes	Homer3
T2565	3410-3411	-LRB-	denotes	(
T2567	3411-3412	CD	denotes	6
T2566	3413-3419	NNS	denotes	clones
T2568	3419-3420	-RRB-	denotes	)
T2569	3421-3424	CC	denotes	and
T2570	3425-3430	NN	denotes	Dncl1
T2571	3431-3432	-LRB-	denotes	(
T2573	3432-3433	CD	denotes	2
T2572	3434-3440	NNS	denotes	clones
T2574	3440-3441	-RRB-	denotes	)
T2575	3442-3446	VBD	denotes	were
T2564	3447-3457	VBN	denotes	identified
T2576	3458-3460	IN	denotes	in
T2577	3461-3464	DT	denotes	the
T2579	3465-3466	NN	denotes	C
T2581	3466-3467	HYPH	denotes	-
T2580	3467-3475	JJ	denotes	terminal
T2582	3476-3483	NN	denotes	peptide
T2578	3484-3490	NN	denotes	screen
T2583	3490-3491	.	denotes	.
T2584	3491-3591	sentence	denotes	Homer3 (7 clones), Dncl1 (2 clones) and Trim11 (6 clones) were identified in the PST domain screen.
T2585	3492-3498	NN	denotes	Homer3
T2587	3499-3500	-LRB-	denotes	(
T2589	3500-3501	CD	denotes	7
T2588	3502-3508	NNS	denotes	clones
T2590	3508-3509	-RRB-	denotes	)
T2591	3509-3511	,	denotes	,
T2592	3511-3516	NN	denotes	Dncl1
T2593	3517-3518	-LRB-	denotes	(
T2595	3518-3519	CD	denotes	2
T2594	3520-3526	NNS	denotes	clones
T2596	3526-3527	-RRB-	denotes	)
T2597	3528-3531	CC	denotes	and
T2598	3532-3538	NN	denotes	Trim11
T2599	3539-3540	-LRB-	denotes	(
T2601	3540-3541	CD	denotes	6
T2600	3542-3548	NNS	denotes	clones
T2602	3548-3549	-RRB-	denotes	)
T2603	3550-3554	VBD	denotes	were
T2586	3555-3565	VBN	denotes	identified
T2604	3566-3568	IN	denotes	in
T2605	3569-3572	DT	denotes	the
T2607	3573-3576	NN	denotes	PST
T2608	3577-3583	NN	denotes	domain
T2606	3584-3590	NN	denotes	screen
T2609	3590-3591	.	denotes	.
T2610	3591-3682	sentence	denotes	All cDNA inserts were in-frame with the coding region of the pPC86 GAL4 activation domain.
T2611	3592-3595	DT	denotes	All
T2613	3596-3600	NN	denotes	cDNA
T2612	3601-3608	NNS	denotes	inserts
T2614	3609-3613	VBD	denotes	were
T2615	3614-3616	IN	denotes	in
T2616	3616-3617	HYPH	denotes	-
T2617	3617-3622	NN	denotes	frame
T2618	3623-3627	IN	denotes	with
T2619	3628-3631	DT	denotes	the
T2621	3632-3638	NN	denotes	coding
T2620	3639-3645	NN	denotes	region
T2622	3646-3648	IN	denotes	of
T2623	3649-3652	DT	denotes	the
T2625	3653-3658	NN	denotes	pPC86
T2626	3659-3663	NN	denotes	GAL4
T2627	3664-3674	NN	denotes	activation
T2624	3675-3681	NN	denotes	domain
T2628	3681-3682	.	denotes	.
T2629	3682-3753	sentence	denotes	None of the cDNAs was present in a list of known false positives [34].
T2630	3683-3687	NN	denotes	None
T2632	3688-3690	IN	denotes	of
T2633	3691-3694	DT	denotes	the
T2634	3695-3700	NNS	denotes	cDNAs
T2631	3701-3704	VBD	denotes	was
T2635	3705-3712	JJ	denotes	present
T2636	3713-3715	IN	denotes	in
T2637	3716-3717	DT	denotes	a
T2638	3718-3722	NN	denotes	list
T2639	3723-3725	IN	denotes	of
T2640	3726-3731	JJ	denotes	known
T2642	3732-3737	JJ	denotes	false
T2641	3738-3747	NNS	denotes	positives
T2643	3748-3749	-LRB-	denotes	[
T2644	3749-3751	CD	denotes	34
T2645	3751-3752	-RRB-	denotes	]
T2646	3752-3753	.	denotes	.
T2647	3753-3847	sentence	denotes	HOMER3 is a member of the HOMER family of neuronal post-synaptic density (PSD) proteins [35].
T2648	3754-3760	NN	denotes	HOMER3
T2649	3761-3763	VBZ	denotes	is
T2650	3764-3765	DT	denotes	a
T2651	3766-3772	NN	denotes	member
T2652	3773-3775	IN	denotes	of
T2653	3776-3779	DT	denotes	the
T2655	3780-3785	NN	denotes	HOMER
T2654	3786-3792	NN	denotes	family
T2656	3793-3795	IN	denotes	of
T2657	3796-3804	JJ	denotes	neuronal
T2659	3805-3818	JJ	denotes	post-synaptic
T2660	3819-3826	NN	denotes	density
T2661	3827-3828	-LRB-	denotes	(
T2662	3828-3831	NN	denotes	PSD
T2663	3831-3832	-RRB-	denotes	)
T2658	3833-3841	NN	denotes	proteins
T2664	3842-3843	-LRB-	denotes	[
T2665	3843-3845	CD	denotes	35
T2666	3845-3846	-RRB-	denotes	]
T2667	3846-3847	.	denotes	.
T2668	3847-4023	sentence	denotes	DNCL1 is a subunit of two motor protein complexes, dynein and myosin-Va, both of which are involved in intracellular trafficking of proteins and organelles in neurons [36,37].
T2669	3848-3853	NN	denotes	DNCL1
T2670	3854-3856	VBZ	denotes	is
T2671	3857-3858	DT	denotes	a
T2672	3859-3866	NN	denotes	subunit
T2673	3867-3869	IN	denotes	of
T2674	3870-3873	CD	denotes	two
T2676	3874-3879	NN	denotes	motor
T2677	3880-3887	NN	denotes	protein
T2675	3888-3897	NNS	denotes	complexes
T2678	3897-3899	,	denotes	,
T2679	3899-3905	NN	denotes	dynein
T2680	3906-3909	CC	denotes	and
T2681	3910-3916	NN	denotes	myosin
T2683	3916-3917	HYPH	denotes	-
T2682	3917-3919	NN	denotes	Va
T2684	3919-3921	,	denotes	,
T2685	3921-3925	DT	denotes	both
T2687	3926-3928	IN	denotes	of
T2688	3929-3934	WDT	denotes	which
T2689	3935-3938	VBP	denotes	are
T2686	3939-3947	VBN	denotes	involved
T2690	3948-3950	IN	denotes	in
T2691	3951-3964	JJ	denotes	intracellular
T2692	3965-3976	NN	denotes	trafficking
T2693	3977-3979	IN	denotes	of
T2694	3980-3988	NN	denotes	proteins
T2695	3989-3992	CC	denotes	and
T2696	3993-4003	NNS	denotes	organelles
T2697	4004-4006	IN	denotes	in
T2698	4007-4014	NNS	denotes	neurons
T2699	4015-4016	-LRB-	denotes	[
T2701	4016-4018	CD	denotes	36
T2702	4018-4019	,	denotes	,
T2700	4019-4021	CD	denotes	37
T2703	4021-4022	-RRB-	denotes	]
T2704	4022-4023	.	denotes	.
T2705	4023-4176	sentence	denotes	TRIM11 is a member of the tripartite motif protein family and contains a RING finger, a B-box zinc finger, a coiled coil domain and a B30.2 domain [38].
T2706	4024-4030	NN	denotes	TRIM11
T2707	4031-4033	VBZ	denotes	is
T2708	4034-4035	DT	denotes	a
T2709	4036-4042	NN	denotes	member
T2710	4043-4045	IN	denotes	of
T2711	4046-4049	DT	denotes	the
T2713	4050-4060	JJ	denotes	tripartite
T2714	4061-4066	NN	denotes	motif
T2715	4067-4074	NN	denotes	protein
T2712	4075-4081	NN	denotes	family
T2716	4082-4085	CC	denotes	and
T2717	4086-4094	VBZ	denotes	contains
T2718	4095-4096	DT	denotes	a
T2720	4097-4101	NN	denotes	RING
T2719	4102-4108	NN	denotes	finger
T2721	4108-4110	,	denotes	,
T2722	4110-4111	DT	denotes	a
T2724	4112-4113	NN	denotes	B
T2726	4113-4114	HYPH	denotes	-
T2725	4114-4117	NN	denotes	box
T2727	4118-4122	NN	denotes	zinc
T2723	4123-4129	NN	denotes	finger
T2728	4129-4131	,	denotes	,
T2729	4131-4132	DT	denotes	a
T2731	4133-4139	VBN	denotes	coiled
T2732	4140-4144	NN	denotes	coil
T2730	4145-4151	NN	denotes	domain
T2733	4152-4155	CC	denotes	and
T2734	4156-4157	DT	denotes	a
T2736	4158-4163	NN	denotes	B30.2
T2735	4164-4170	NN	denotes	domain
T2737	4171-4172	-LRB-	denotes	[
T2738	4172-4174	CD	denotes	38
T2739	4174-4175	-RRB-	denotes	]
T2740	4175-4176	.	denotes	.
T2741	4176-4274	sentence	denotes	The possible significance of the interactions between these proteins and PAX6 is discussed below.
T2742	4177-4180	DT	denotes	The
T2744	4181-4189	JJ	denotes	possible
T2743	4190-4202	NN	denotes	significance
T2746	4203-4205	IN	denotes	of
T2747	4206-4209	DT	denotes	the
T2748	4210-4222	NNS	denotes	interactions
T2749	4223-4230	IN	denotes	between
T2750	4231-4236	DT	denotes	these
T2751	4237-4245	NN	denotes	proteins
T2752	4246-4249	CC	denotes	and
T2753	4250-4254	NN	denotes	PAX6
T2754	4255-4257	VBZ	denotes	is
T2745	4258-4267	VBN	denotes	discussed
T2755	4268-4273	RB	denotes	below
T2756	4273-4274	.	denotes	.
T2839	4276-4293	JJ	denotes	Semi-quantitative
T2840	4294-4297	NN	denotes	PCR
T2841	4297-4482	sentence	denotes	To check that the Homer3, Dncl1 and Trim11 clones were not identified multiple times solely because they are highly abundant in the library, we performed a semi-quantitative PCR assay.
T2842	4298-4300	TO	denotes	To
T2843	4301-4306	VB	denotes	check
T2845	4307-4311	IN	denotes	that
T2847	4312-4315	DT	denotes	the
T2849	4316-4322	NN	denotes	Homer3
T2850	4322-4324	,	denotes	,
T2851	4324-4329	NN	denotes	Dncl1
T2852	4330-4333	CC	denotes	and
T2853	4334-4340	NN	denotes	Trim11
T2848	4341-4347	NNS	denotes	clones
T2854	4348-4352	VBD	denotes	were
T2855	4353-4356	RB	denotes	not
T2846	4357-4367	VBN	denotes	identified
T2856	4368-4376	JJ	denotes	multiple
T2857	4377-4382	NNS	denotes	times
T2858	4383-4389	RB	denotes	solely
T2860	4390-4397	IN	denotes	because
T2861	4398-4402	PRP	denotes	they
T2859	4403-4406	VBP	denotes	are
T2862	4407-4413	RB	denotes	highly
T2863	4414-4422	JJ	denotes	abundant
T2864	4423-4425	IN	denotes	in
T2865	4426-4429	DT	denotes	the
T2866	4430-4437	NN	denotes	library
T2867	4437-4439	,	denotes	,
T2868	4439-4441	PRP	denotes	we
T2844	4442-4451	VBD	denotes	performed
T2869	4452-4453	DT	denotes	a
T2871	4454-4471	JJ	denotes	semi-quantitative
T2872	4472-4475	NN	denotes	PCR
T2870	4476-4481	NN	denotes	assay
T2873	4481-4482	.	denotes	.
T2874	4482-4574	sentence	denotes	We compared the relative abundance of Homer3, Dncl1, Trim11 and Pax6 with Gapdh and Atp5a1.
T2875	4483-4485	PRP	denotes	We
T2876	4486-4494	VBD	denotes	compared
T2877	4495-4498	DT	denotes	the
T2879	4499-4507	JJ	denotes	relative
T2878	4508-4517	NN	denotes	abundance
T2880	4518-4520	IN	denotes	of
T2881	4521-4527	NN	denotes	Homer3
T2882	4527-4529	,	denotes	,
T2883	4529-4534	NN	denotes	Dncl1
T2884	4534-4536	,	denotes	,
T2885	4536-4542	NN	denotes	Trim11
T2886	4543-4546	CC	denotes	and
T2887	4547-4551	NN	denotes	Pax6
T2888	4552-4556	IN	denotes	with
T2889	4557-4562	NN	denotes	Gapdh
T2890	4563-4566	CC	denotes	and
T2891	4567-4573	NN	denotes	Atp5a1
T2892	4573-4574	.	denotes	.
T2893	4574-4685	sentence	denotes	Gapdh and Atp5a1 both show strong constitutive expression in a variety of tissues including the brain [29,30].
T2894	4575-4580	NN	denotes	Gapdh
T2896	4581-4584	CC	denotes	and
T2897	4585-4591	NN	denotes	Atp5a1
T2898	4592-4596	DT	denotes	both
T2895	4597-4601	VBP	denotes	show
T2899	4602-4608	JJ	denotes	strong
T2901	4609-4621	JJ	denotes	constitutive
T2900	4622-4632	NN	denotes	expression
T2902	4633-4635	IN	denotes	in
T2903	4636-4637	DT	denotes	a
T2904	4638-4645	NN	denotes	variety
T2905	4646-4648	IN	denotes	of
T2906	4649-4656	NNS	denotes	tissues
T2907	4657-4666	VBG	denotes	including
T2908	4667-4670	DT	denotes	the
T2909	4671-4676	NN	denotes	brain
T2910	4677-4678	-LRB-	denotes	[
T2912	4678-4680	CD	denotes	29
T2913	4680-4681	,	denotes	,
T2911	4681-4683	CD	denotes	30
T2914	4683-4684	-RRB-	denotes	]
T2915	4684-4685	.	denotes	.
T2916	4685-4945	sentence	denotes	Homer3, Dncl1, Trim11 and Pax6 were only amplified strongly after 35 cycles of PCR (Figure 2) and were therefore present at relatively low levels compared to Gapdh (amplified strongly after 25 cycles) and Atp5a1 (amplified strongly after 30 cycles; Figure 2).
T2917	4686-4692	NN	denotes	Homer3
T2919	4692-4694	,	denotes	,
T2920	4694-4699	NN	denotes	Dncl1
T2921	4699-4701	,	denotes	,
T2922	4701-4707	NN	denotes	Trim11
T2923	4708-4711	CC	denotes	and
T2924	4712-4716	NN	denotes	Pax6
T2925	4717-4721	VBD	denotes	were
T2926	4722-4726	RB	denotes	only
T2918	4727-4736	VBN	denotes	amplified
T2927	4737-4745	RB	denotes	strongly
T2928	4746-4751	IN	denotes	after
T2929	4752-4754	CD	denotes	35
T2930	4755-4761	NNS	denotes	cycles
T2931	4762-4764	IN	denotes	of
T2932	4765-4768	NN	denotes	PCR
T2933	4769-4770	-LRB-	denotes	(
T2934	4770-4776	NN	denotes	Figure
T2935	4777-4778	CD	denotes	2
T2936	4778-4779	-RRB-	denotes	)
T2937	4780-4783	CC	denotes	and
T2938	4784-4788	VBD	denotes	were
T2939	4789-4798	RB	denotes	therefore
T2940	4799-4806	JJ	denotes	present
T2941	4807-4809	IN	denotes	at
T2942	4810-4820	RB	denotes	relatively
T2943	4821-4824	JJ	denotes	low
T2944	4825-4831	NNS	denotes	levels
T2945	4832-4840	VBN	denotes	compared
T2946	4841-4843	IN	denotes	to
T2947	4844-4849	NN	denotes	Gapdh
T2948	4850-4851	-LRB-	denotes	(
T2949	4851-4860	VBN	denotes	amplified
T2950	4861-4869	RB	denotes	strongly
T2951	4870-4875	IN	denotes	after
T2952	4876-4878	CD	denotes	25
T2953	4879-4885	NNS	denotes	cycles
T2954	4885-4886	-RRB-	denotes	)
T2955	4887-4890	CC	denotes	and
T2956	4891-4897	NN	denotes	Atp5a1
T2957	4898-4899	-LRB-	denotes	(
T2958	4899-4908	VBN	denotes	amplified
T2959	4909-4917	RB	denotes	strongly
T2960	4918-4923	IN	denotes	after
T2961	4924-4926	CD	denotes	30
T2962	4927-4933	NNS	denotes	cycles
T2963	4933-4934	:	denotes	;
T2964	4935-4941	NN	denotes	Figure
T2965	4942-4943	CD	denotes	2
T2966	4943-4944	-RRB-	denotes	)
T2967	4944-4945	.	denotes	.
T2968	4945-5517	sentence	denotes	Figure 2 Semi-quantitative PCR analysis of Homer3, Dncl1, Trim11 and Pax6 in the mouse brain cDNA library. Library cDNA was amplified with primers specific for Pax6 (600 bp), Homer3 (485 bp band), Dncl1 (485 bp) and Trim11 (609 bp) for 20, 25, 30 or 35 cycles. Atp5a1 (415 bp) and Gapdh (450 bp), which are highly expressed in the brain, are included for comparison. M indicates the Φ×174 HaeIII DNA size marker; the positions of the 603 bp and 310 bp marker bands are indicated. We concluded that Homer3, Dncl1 and Trim11 clones were not highly abundant in the library.
T8468	4956-4973	JJ	denotes	Semi-quantitative
T8470	4974-4977	NN	denotes	PCR
T8469	4978-4986	NN	denotes	analysis
T8471	4987-4989	IN	denotes	of
T8472	4990-4996	NN	denotes	Homer3
T8473	4996-4998	,	denotes	,
T8474	4998-5003	NN	denotes	Dncl1
T8475	5003-5005	,	denotes	,
T8476	5005-5011	NN	denotes	Trim11
T8477	5012-5015	CC	denotes	and
T8478	5016-5020	NN	denotes	Pax6
T8479	5021-5023	IN	denotes	in
T8480	5024-5027	DT	denotes	the
T8482	5028-5033	NN	denotes	mouse
T8483	5034-5039	NN	denotes	brain
T8484	5040-5044	NN	denotes	cDNA
T8481	5045-5052	NN	denotes	library
T8485	5052-5053	.	denotes	.
T8486	5053-5207	sentence	denotes	Library cDNA was amplified with primers specific for Pax6 (600 bp), Homer3 (485 bp band), Dncl1 (485 bp) and Trim11 (609 bp) for 20, 25, 30 or 35 cycles.
T8487	5054-5061	NN	denotes	Library
T8488	5062-5066	NN	denotes	cDNA
T8490	5067-5070	VBD	denotes	was
T8489	5071-5080	VBN	denotes	amplified
T8491	5081-5085	IN	denotes	with
T8492	5086-5093	NNS	denotes	primers
T8493	5094-5102	JJ	denotes	specific
T8494	5103-5106	IN	denotes	for
T8495	5107-5111	NN	denotes	Pax6
T8496	5112-5113	-LRB-	denotes	(
T8498	5113-5116	CD	denotes	600
T8497	5117-5119	NN	denotes	bp
T8499	5119-5120	-RRB-	denotes	)
T8500	5120-5122	,	denotes	,
T8501	5122-5128	NN	denotes	Homer3
T8502	5129-5130	-LRB-	denotes	(
T8504	5130-5133	CD	denotes	485
T8505	5134-5136	NN	denotes	bp
T8503	5137-5141	NN	denotes	band
T8506	5141-5142	-RRB-	denotes	)
T8507	5142-5144	,	denotes	,
T8508	5144-5149	NN	denotes	Dncl1
T8509	5150-5151	-LRB-	denotes	(
T8511	5151-5154	CD	denotes	485
T8510	5155-5157	NN	denotes	bp
T8512	5157-5158	-RRB-	denotes	)
T8513	5159-5162	CC	denotes	and
T8514	5163-5169	NN	denotes	Trim11
T8515	5170-5171	-LRB-	denotes	(
T8517	5171-5174	CD	denotes	609
T8516	5175-5177	NN	denotes	bp
T8518	5177-5178	-RRB-	denotes	)
T8519	5179-5182	IN	denotes	for
T8520	5183-5185	CD	denotes	20
T8522	5185-5187	,	denotes	,
T8523	5187-5189	CD	denotes	25
T8524	5189-5191	,	denotes	,
T8525	5191-5193	CD	denotes	30
T8526	5194-5196	CC	denotes	or
T8527	5197-5199	CD	denotes	35
T8521	5200-5206	NNS	denotes	cycles
T8528	5206-5207	.	denotes	.
T8529	5207-5313	sentence	denotes	Atp5a1 (415 bp) and Gapdh (450 bp), which are highly expressed in the brain, are included for comparison.
T8530	5208-5214	NN	denotes	Atp5a1
T8532	5215-5216	-LRB-	denotes	(
T8534	5216-5219	CD	denotes	415
T8533	5220-5222	NN	denotes	bp
T8535	5222-5223	-RRB-	denotes	)
T8536	5224-5227	CC	denotes	and
T8537	5228-5233	NN	denotes	Gapdh
T8538	5234-5235	-LRB-	denotes	(
T8540	5235-5238	CD	denotes	450
T8539	5239-5241	NN	denotes	bp
T8541	5241-5242	-RRB-	denotes	)
T8542	5242-5244	,	denotes	,
T8543	5244-5249	WDT	denotes	which
T8545	5250-5253	VBP	denotes	are
T8546	5254-5260	RB	denotes	highly
T8544	5261-5270	VBN	denotes	expressed
T8547	5271-5273	IN	denotes	in
T8548	5274-5277	DT	denotes	the
T8549	5278-5283	NN	denotes	brain
T8550	5283-5285	,	denotes	,
T8551	5285-5288	VBP	denotes	are
T8531	5289-5297	VBN	denotes	included
T8552	5298-5301	IN	denotes	for
T8553	5302-5312	NN	denotes	comparison
T8554	5312-5313	.	denotes	.
T8555	5313-5426	sentence	denotes	M indicates the Φ×174 HaeIII DNA size marker; the positions of the 603 bp and 310 bp marker bands are indicated.
T8556	5314-5315	NN	denotes	M
T8557	5316-5325	VBZ	denotes	indicates
T8559	5326-5329	DT	denotes	the
T8561	5330-5332	NN	denotes	Φ×
T8563	5332-5335	CD	denotes	174
T8562	5336-5342	NN	denotes	HaeIII
T8564	5343-5346	NN	denotes	DNA
T8565	5347-5351	NN	denotes	size
T8560	5352-5358	NN	denotes	marker
T8566	5358-5359	:	denotes	;
T8567	5360-5363	DT	denotes	the
T8568	5364-5373	NNS	denotes	positions
T8569	5374-5376	IN	denotes	of
T8570	5377-5380	DT	denotes	the
T8572	5381-5384	CD	denotes	603
T8573	5385-5387	NN	denotes	bp
T8574	5388-5391	CC	denotes	and
T8575	5392-5395	CD	denotes	310
T8576	5396-5398	NN	denotes	bp
T8577	5399-5405	NN	denotes	marker
T8571	5406-5411	NNS	denotes	bands
T8578	5412-5415	VBP	denotes	are
T8558	5416-5425	VBN	denotes	indicated
T8579	5425-5426	.	denotes	.
T2969	5427-5429	PRP	denotes	We
T2970	5430-5439	VBD	denotes	concluded
T2971	5440-5444	IN	denotes	that
T2973	5445-5451	NN	denotes	Homer3
T2975	5451-5453	,	denotes	,
T2976	5453-5458	NN	denotes	Dncl1
T2977	5459-5462	CC	denotes	and
T2978	5463-5469	NN	denotes	Trim11
T2974	5470-5476	NNS	denotes	clones
T2972	5477-5481	VBD	denotes	were
T2979	5482-5485	RB	denotes	not
T2980	5486-5492	RB	denotes	highly
T2981	5493-5501	JJ	denotes	abundant
T2982	5502-5504	IN	denotes	in
T2983	5505-5508	DT	denotes	the
T2984	5509-5516	NN	denotes	library
T2985	5516-5517	.	denotes	.
T2986	5517-5678	sentence	denotes	This is consistent with the idea that they were pulled out because the encoded proteins interact specifically with the C-terminal peptide or PST domain of PAX6.
T2987	5518-5522	DT	denotes	This
T2988	5523-5525	VBZ	denotes	is
T2989	5526-5536	JJ	denotes	consistent
T2990	5537-5541	IN	denotes	with
T2991	5542-5545	DT	denotes	the
T2992	5546-5550	NN	denotes	idea
T2993	5551-5555	IN	denotes	that
T2995	5556-5560	PRP	denotes	they
T2996	5561-5565	VBD	denotes	were
T2994	5566-5572	VBN	denotes	pulled
T2997	5573-5576	RP	denotes	out
T2998	5577-5584	IN	denotes	because
T3000	5585-5588	DT	denotes	the
T3002	5589-5596	VBN	denotes	encoded
T3001	5597-5605	NN	denotes	proteins
T2999	5606-5614	VBP	denotes	interact
T3003	5615-5627	RB	denotes	specifically
T3004	5628-5632	IN	denotes	with
T3005	5633-5636	DT	denotes	the
T3007	5637-5638	NN	denotes	C
T3009	5638-5639	HYPH	denotes	-
T3008	5639-5647	JJ	denotes	terminal
T3006	5648-5655	NN	denotes	peptide
T3010	5656-5658	CC	denotes	or
T3011	5659-5662	NN	denotes	PST
T3012	5663-5669	NN	denotes	domain
T3013	5670-5672	IN	denotes	of
T3014	5673-5677	NN	denotes	PAX6
T3015	5677-5678	.	denotes	.
T3382	5680-5685	NN	denotes	Yeast
T3384	5686-5689	CD	denotes	two
T3386	5689-5690	HYPH	denotes	-
T3385	5690-5696	NN	denotes	hybrid
T3387	5697-5705	JJ	denotes	pairwise
T3383	5706-5718	NNS	denotes	interactions
T3388	5718-5914	sentence	denotes	By library screening we identified two proteins (HOMER3 and DNCL1) that interact with the C-terminal peptide and three proteins (HOMER3, DNCL1 and TRIM11) that interact with the whole PST domain.
T3389	5719-5721	IN	denotes	By
T3391	5722-5729	NN	denotes	library
T3392	5730-5739	NN	denotes	screening
T3393	5740-5742	PRP	denotes	we
T3390	5743-5753	VBD	denotes	identified
T3394	5754-5757	CD	denotes	two
T3395	5758-5766	NN	denotes	proteins
T3396	5767-5768	-LRB-	denotes	(
T3397	5768-5774	NN	denotes	HOMER3
T3398	5775-5778	CC	denotes	and
T3399	5779-5784	NN	denotes	DNCL1
T3400	5784-5785	-RRB-	denotes	)
T3401	5786-5790	WDT	denotes	that
T3402	5791-5799	VBP	denotes	interact
T3403	5800-5804	IN	denotes	with
T3404	5805-5808	DT	denotes	the
T3406	5809-5810	NN	denotes	C
T3408	5810-5811	HYPH	denotes	-
T3407	5811-5819	JJ	denotes	terminal
T3405	5820-5827	NN	denotes	peptide
T3409	5828-5831	CC	denotes	and
T3410	5832-5837	CD	denotes	three
T3411	5838-5846	NN	denotes	proteins
T3412	5847-5848	-LRB-	denotes	(
T3413	5848-5854	NN	denotes	HOMER3
T3414	5854-5856	,	denotes	,
T3415	5856-5861	NN	denotes	DNCL1
T3416	5862-5865	CC	denotes	and
T3417	5866-5872	NN	denotes	TRIM11
T3418	5872-5873	-RRB-	denotes	)
T3419	5874-5878	WDT	denotes	that
T3420	5879-5887	VBP	denotes	interact
T3421	5888-5892	IN	denotes	with
T3422	5893-5896	DT	denotes	the
T3424	5897-5902	JJ	denotes	whole
T3425	5903-5906	NN	denotes	PST
T3423	5907-5913	NN	denotes	domain
T3426	5913-5914	.	denotes	.
T3427	5914-6070	sentence	denotes	This suggests that HOMER3 and DNCL1 interact specifically with the C-terminal peptide while TRIM11 interacts with a more N-terminal part of the PST domain.
T3428	5915-5919	DT	denotes	This
T3429	5920-5928	VBZ	denotes	suggests
T3430	5929-5933	IN	denotes	that
T3432	5934-5940	NN	denotes	HOMER3
T3433	5941-5944	CC	denotes	and
T3434	5945-5950	NN	denotes	DNCL1
T3431	5951-5959	VBP	denotes	interact
T3435	5960-5972	RB	denotes	specifically
T3436	5973-5977	IN	denotes	with
T3437	5978-5981	DT	denotes	the
T3439	5982-5983	NN	denotes	C
T3441	5983-5984	HYPH	denotes	-
T3440	5984-5992	JJ	denotes	terminal
T3438	5993-6000	NN	denotes	peptide
T3442	6001-6006	IN	denotes	while
T3444	6007-6013	NN	denotes	TRIM11
T3443	6014-6023	VBZ	denotes	interacts
T3445	6024-6028	IN	denotes	with
T3446	6029-6030	DT	denotes	a
T3448	6031-6035	RBR	denotes	more
T3450	6036-6037	NN	denotes	N
T3451	6037-6038	HYPH	denotes	-
T3449	6038-6046	JJ	denotes	terminal
T3447	6047-6051	NN	denotes	part
T3452	6052-6054	IN	denotes	of
T3453	6055-6058	DT	denotes	the
T3455	6059-6062	NN	denotes	PST
T3454	6063-6069	NN	denotes	domain
T3456	6069-6070	.	denotes	.
T3457	6070-6273	sentence	denotes	We conducted pairwise tests between specific constructs to confirm the interactions identified in the library screen and to further investigate the interaction between PAX6 and HOMER3, DNCL1 and TRIM11.
T3458	6071-6073	PRP	denotes	We
T3459	6074-6083	VBD	denotes	conducted
T3460	6084-6092	JJ	denotes	pairwise
T3461	6093-6098	NNS	denotes	tests
T3462	6099-6106	IN	denotes	between
T3463	6107-6115	JJ	denotes	specific
T3464	6116-6126	NNS	denotes	constructs
T3465	6127-6129	TO	denotes	to
T3466	6130-6137	VB	denotes	confirm
T3467	6138-6141	DT	denotes	the
T3468	6142-6154	NNS	denotes	interactions
T3469	6155-6165	VBN	denotes	identified
T3470	6166-6168	IN	denotes	in
T3471	6169-6172	DT	denotes	the
T3473	6173-6180	NN	denotes	library
T3472	6181-6187	NN	denotes	screen
T3474	6188-6191	CC	denotes	and
T3475	6192-6194	TO	denotes	to
T3477	6195-6202	RB	denotes	further
T3476	6203-6214	VB	denotes	investigate
T3478	6215-6218	DT	denotes	the
T3479	6219-6230	NN	denotes	interaction
T3480	6231-6238	IN	denotes	between
T3481	6239-6243	NN	denotes	PAX6
T3482	6244-6247	CC	denotes	and
T3483	6248-6254	NN	denotes	HOMER3
T3484	6254-6256	,	denotes	,
T3485	6256-6261	NN	denotes	DNCL1
T3486	6262-6265	CC	denotes	and
T3487	6266-6272	NN	denotes	TRIM11
T3488	6272-6273	.	denotes	.
T3489	6273-6414	sentence	denotes	The Dncl1 and Trim11 clones that were pulled out of the library were full-length, but the Homer3 cDNAs lacked the N-terminal 70 amino acids.
T3490	6274-6277	DT	denotes	The
T3492	6278-6283	NN	denotes	Dncl1
T3493	6284-6287	CC	denotes	and
T3494	6288-6294	NN	denotes	Trim11
T3491	6295-6301	NNS	denotes	clones
T3496	6302-6306	WDT	denotes	that
T3498	6307-6311	VBD	denotes	were
T3497	6312-6318	VBN	denotes	pulled
T3499	6319-6322	IN	denotes	out
T3500	6323-6325	IN	denotes	of
T3501	6326-6329	DT	denotes	the
T3502	6330-6337	NN	denotes	library
T3495	6338-6342	VBD	denotes	were
T3503	6343-6347	JJ	denotes	full
T3505	6347-6348	HYPH	denotes	-
T3504	6348-6354	NN	denotes	length
T3506	6354-6356	,	denotes	,
T3507	6356-6359	CC	denotes	but
T3508	6360-6363	DT	denotes	the
T3510	6364-6370	NN	denotes	Homer3
T3509	6371-6376	NNS	denotes	cDNAs
T3511	6377-6383	VBD	denotes	lacked
T3512	6384-6387	DT	denotes	the
T3514	6388-6389	NN	denotes	N
T3516	6389-6390	HYPH	denotes	-
T3515	6390-6398	JJ	denotes	terminal
T3517	6399-6401	CD	denotes	70
T3518	6402-6407	NN	denotes	amino
T3513	6408-6413	NNS	denotes	acids
T3519	6413-6414	.	denotes	.
T3520	6414-6542	sentence	denotes	The missing coding region was inserted into the truncated Homer3 cDNA to give a full-length expression construct (see Methods).
T3521	6415-6418	DT	denotes	The
T3523	6419-6426	JJ	denotes	missing
T3524	6427-6433	NN	denotes	coding
T3522	6434-6440	NN	denotes	region
T3526	6441-6444	VBD	denotes	was
T3525	6445-6453	VBN	denotes	inserted
T3527	6454-6458	IN	denotes	into
T3528	6459-6462	DT	denotes	the
T3530	6463-6472	VBN	denotes	truncated
T3531	6473-6479	NN	denotes	Homer3
T3529	6480-6484	NN	denotes	cDNA
T3532	6485-6487	TO	denotes	to
T3533	6488-6492	VB	denotes	give
T3534	6493-6494	DT	denotes	a
T3536	6495-6499	JJ	denotes	full
T3538	6499-6500	HYPH	denotes	-
T3537	6500-6506	NN	denotes	length
T3539	6507-6517	NN	denotes	expression
T3535	6518-6527	NN	denotes	construct
T3540	6528-6529	-LRB-	denotes	(
T3541	6529-6532	VB	denotes	see
T3542	6533-6540	NNS	denotes	Methods
T3543	6540-6541	-RRB-	denotes	)
T3544	6541-6542	.	denotes	.
T3545	6542-6636	sentence	denotes	Pairwise interactions were carried out with both the full-length and truncated Homer3 clones.
T3546	6543-6551	JJ	denotes	Pairwise
T3547	6552-6564	NNS	denotes	interactions
T3549	6565-6569	VBD	denotes	were
T3548	6570-6577	VBN	denotes	carried
T3550	6578-6581	RP	denotes	out
T3551	6582-6586	IN	denotes	with
T3552	6587-6591	CC	denotes	both
T3554	6592-6595	DT	denotes	the
T3555	6596-6600	JJ	denotes	full
T3557	6600-6601	HYPH	denotes	-
T3556	6601-6607	NN	denotes	length
T3558	6608-6611	CC	denotes	and
T3559	6612-6621	VBN	denotes	truncated
T3560	6622-6628	NN	denotes	Homer3
T3553	6629-6635	NNS	denotes	clones
T3561	6635-6636	.	denotes	.
T3562	6636-6851	sentence	denotes	We confirmed that the whole PAX6 PST domain interacts with HOMER3 (full-length and truncated constructs), TRIM11 and DNCL1, as all three reporter genes were strongly activated in pairwise tests (Figure 3; Table 1).
T3563	6637-6639	PRP	denotes	We
T3564	6640-6649	VBD	denotes	confirmed
T3565	6650-6654	IN	denotes	that
T3567	6655-6658	DT	denotes	the
T3569	6659-6664	JJ	denotes	whole
T3570	6665-6669	NN	denotes	PAX6
T3571	6670-6673	NN	denotes	PST
T3568	6674-6680	NN	denotes	domain
T3566	6681-6690	VBZ	denotes	interacts
T3572	6691-6695	IN	denotes	with
T3573	6696-6702	NN	denotes	HOMER3
T3574	6703-6704	-LRB-	denotes	(
T3576	6704-6708	JJ	denotes	full
T3578	6708-6709	HYPH	denotes	-
T3577	6709-6715	NN	denotes	length
T3579	6716-6719	CC	denotes	and
T3580	6720-6729	VBN	denotes	truncated
T3575	6730-6740	NNS	denotes	constructs
T3581	6740-6741	-RRB-	denotes	)
T3582	6741-6743	,	denotes	,
T3583	6743-6749	NN	denotes	TRIM11
T3584	6750-6753	CC	denotes	and
T3585	6754-6759	NN	denotes	DNCL1
T3586	6759-6761	,	denotes	,
T3587	6761-6763	IN	denotes	as
T3589	6764-6767	DT	denotes	all
T3591	6768-6773	CD	denotes	three
T3592	6774-6782	NN	denotes	reporter
T3590	6783-6788	NNS	denotes	genes
T3593	6789-6793	VBD	denotes	were
T3594	6794-6802	RB	denotes	strongly
T3588	6803-6812	VBN	denotes	activated
T3595	6813-6815	IN	denotes	in
T3596	6816-6824	JJ	denotes	pairwise
T3597	6825-6830	NNS	denotes	tests
T3598	6831-6832	-LRB-	denotes	(
T3600	6832-6838	NN	denotes	Figure
T3601	6839-6840	CD	denotes	3
T3602	6840-6841	:	denotes	;
T3599	6842-6847	NN	denotes	Table
T3603	6848-6849	CD	denotes	1
T3604	6849-6850	-RRB-	denotes	)
T3605	6850-6851	.	denotes	.
T3606	6851-6984	sentence	denotes	In contrast the interaction between the C-terminal peptide and HOMER3 or DNCL1 could not be confirmed with pairwise tests (Table 1).
T3607	6852-6854	IN	denotes	In
T3609	6855-6863	NN	denotes	contrast
T3610	6864-6867	DT	denotes	the
T3611	6868-6879	NN	denotes	interaction
T3612	6880-6887	IN	denotes	between
T3613	6888-6891	DT	denotes	the
T3615	6892-6893	NN	denotes	C
T3617	6893-6894	HYPH	denotes	-
T3616	6894-6902	JJ	denotes	terminal
T3614	6903-6910	NN	denotes	peptide
T3618	6911-6914	CC	denotes	and
T3619	6915-6921	NN	denotes	HOMER3
T3620	6922-6924	CC	denotes	or
T3621	6925-6930	NN	denotes	DNCL1
T3622	6931-6936	MD	denotes	could
T3623	6937-6940	RB	denotes	not
T3624	6941-6943	VB	denotes	be
T3608	6944-6953	VBN	denotes	confirmed
T3625	6954-6958	IN	denotes	with
T3626	6959-6967	JJ	denotes	pairwise
T3627	6968-6973	NNS	denotes	tests
T3628	6974-6975	-LRB-	denotes	(
T3629	6975-6980	NN	denotes	Table
T3630	6981-6982	CD	denotes	1
T3631	6982-6983	-RRB-	denotes	)
T3632	6983-6984	.	denotes	.
T3633	6984-7190	sentence	denotes	Occasionally, partial suppression of growth on plates containing 5-fluoro-orotic acid was observed, indicating low-level activation of the URA3 reporter; however HIS3 and LacZ activation were not observed.
T3634	6985-6997	RB	denotes	Occasionally
T3636	6997-6999	,	denotes	,
T3637	6999-7006	JJ	denotes	partial
T3638	7007-7018	NN	denotes	suppression
T3639	7019-7021	IN	denotes	of
T3640	7022-7028	NN	denotes	growth
T3641	7029-7031	IN	denotes	on
T3642	7032-7038	NNS	denotes	plates
T3643	7039-7049	VBG	denotes	containing
T3644	7050-7051	CD	denotes	5
T3646	7051-7052	HYPH	denotes	-
T3647	7052-7058	JJ	denotes	fluoro
T3648	7058-7059	HYPH	denotes	-
T3645	7059-7065	JJ	denotes	orotic
T3649	7066-7070	NN	denotes	acid
T3650	7071-7074	VBD	denotes	was
T3635	7075-7083	VBN	denotes	observed
T3652	7083-7085	,	denotes	,
T3653	7085-7095	VBG	denotes	indicating
T3654	7096-7099	JJ	denotes	low
T3656	7099-7100	HYPH	denotes	-
T3655	7100-7105	NN	denotes	level
T3657	7106-7116	NN	denotes	activation
T3658	7117-7119	IN	denotes	of
T3659	7120-7123	DT	denotes	the
T3661	7124-7128	NN	denotes	URA3
T3660	7129-7137	NN	denotes	reporter
T3662	7137-7138	:	denotes	;
T3663	7139-7146	RB	denotes	however
T3664	7147-7151	NN	denotes	HIS3
T3666	7152-7155	CC	denotes	and
T3667	7156-7160	NN	denotes	LacZ
T3665	7161-7171	NN	denotes	activation
T3668	7172-7176	VBD	denotes	were
T3669	7177-7180	RB	denotes	not
T3651	7181-7189	VBN	denotes	observed
T3670	7189-7190	.	denotes	.
T3671	7190-7333	sentence	denotes	The reasons for this are not clear, although it may be that the pairwise tests were of sub-optimal sensitivity compared to the library screen.
T3672	7191-7194	DT	denotes	The
T3673	7195-7202	NNS	denotes	reasons
T3675	7203-7206	IN	denotes	for
T3676	7207-7211	DT	denotes	this
T3674	7212-7215	VBP	denotes	are
T3677	7216-7219	RB	denotes	not
T3678	7220-7225	JJ	denotes	clear
T3679	7225-7227	,	denotes	,
T3680	7227-7235	IN	denotes	although
T3682	7236-7238	PRP	denotes	it
T3683	7239-7242	MD	denotes	may
T3681	7243-7245	VB	denotes	be
T3684	7246-7250	IN	denotes	that
T3686	7251-7254	DT	denotes	the
T3688	7255-7263	JJ	denotes	pairwise
T3687	7264-7269	NNS	denotes	tests
T3685	7270-7274	VBD	denotes	were
T3689	7275-7277	IN	denotes	of
T3690	7278-7289	JJ	denotes	sub-optimal
T3691	7290-7301	NN	denotes	sensitivity
T3692	7302-7310	VBN	denotes	compared
T3693	7311-7313	IN	denotes	to
T3694	7314-7317	DT	denotes	the
T3696	7318-7325	NN	denotes	library
T3695	7326-7332	NN	denotes	screen
T3697	7332-7333	.	denotes	.
T3698	7333-7583	sentence	denotes	However we were able to confirm that the C-terminal peptide is important for the interaction with HOMER3 and DNCL1 because interaction with the PAX6PST-CT construct, which lacks the final 32 amino acids, was completely abolished (Figure 3, Table 1).
T3699	7334-7341	RB	denotes	However
T3701	7342-7344	PRP	denotes	we
T3700	7345-7349	VBD	denotes	were
T3702	7350-7354	JJ	denotes	able
T3703	7355-7357	TO	denotes	to
T3704	7358-7365	VB	denotes	confirm
T3705	7366-7370	IN	denotes	that
T3707	7371-7374	DT	denotes	the
T3709	7375-7376	NN	denotes	C
T3711	7376-7377	HYPH	denotes	-
T3710	7377-7385	JJ	denotes	terminal
T3708	7386-7393	NN	denotes	peptide
T3706	7394-7396	VBZ	denotes	is
T3712	7397-7406	JJ	denotes	important
T3713	7407-7410	IN	denotes	for
T3714	7411-7414	DT	denotes	the
T3715	7415-7426	NN	denotes	interaction
T3716	7427-7431	IN	denotes	with
T3717	7432-7438	NN	denotes	HOMER3
T3718	7439-7442	CC	denotes	and
T3719	7443-7448	NN	denotes	DNCL1
T3720	7449-7456	IN	denotes	because
T3722	7457-7468	NN	denotes	interaction
T3723	7469-7473	IN	denotes	with
T3724	7474-7477	DT	denotes	the
T3726	7478-7485	NN	denotes	PAX6PST
T3728	7485-7486	HYPH	denotes	-
T3727	7486-7488	NN	denotes	CT
T3725	7489-7498	NN	denotes	construct
T3729	7498-7500	,	denotes	,
T3730	7500-7505	WDT	denotes	which
T3731	7506-7511	VBZ	denotes	lacks
T3732	7512-7515	DT	denotes	the
T3734	7516-7521	JJ	denotes	final
T3735	7522-7524	CD	denotes	32
T3736	7525-7530	NN	denotes	amino
T3733	7531-7536	NNS	denotes	acids
T3737	7536-7538	,	denotes	,
T3738	7538-7541	VBD	denotes	was
T3739	7542-7552	RB	denotes	completely
T3721	7553-7562	VBN	denotes	abolished
T3740	7563-7564	-LRB-	denotes	(
T3742	7564-7570	NN	denotes	Figure
T3743	7571-7572	CD	denotes	3
T3744	7572-7574	,	denotes	,
T3741	7574-7579	NN	denotes	Table
T3745	7580-7581	CD	denotes	1
T3746	7581-7582	-RRB-	denotes	)
T3747	7582-7583	.	denotes	.
T3748	7583-7663	sentence	denotes	TRIM11 interacted equally well with PAX6PST and PAX6PST-CT (Figure 3, Table 1).
T3749	7584-7590	NN	denotes	TRIM11
T3750	7591-7601	VBD	denotes	interacted
T3751	7602-7609	RB	denotes	equally
T3752	7610-7614	RB	denotes	well
T3753	7615-7619	IN	denotes	with
T3754	7620-7627	NN	denotes	PAX6PST
T3755	7628-7631	CC	denotes	and
T3756	7632-7639	NN	denotes	PAX6PST
T3758	7639-7640	HYPH	denotes	-
T3757	7640-7642	NN	denotes	CT
T3759	7643-7644	-LRB-	denotes	(
T3761	7644-7650	NN	denotes	Figure
T3762	7651-7652	CD	denotes	3
T3763	7652-7654	,	denotes	,
T3760	7654-7659	NN	denotes	Table
T3764	7660-7661	CD	denotes	1
T3765	7661-7662	-RRB-	denotes	)
T3766	7662-7663	.	denotes	.
T3767	7663-7904	sentence	denotes	This is consistent with the library screens in which TRIM11 was isolated with the PST domain but not with the C-terminal peptide and supports the idea that the C-terminal peptide is not important for the interaction between PAX6 and TRIM11.
T3768	7664-7668	DT	denotes	This
T3769	7669-7671	VBZ	denotes	is
T3770	7672-7682	JJ	denotes	consistent
T3771	7683-7687	IN	denotes	with
T3772	7688-7691	DT	denotes	the
T3774	7692-7699	NN	denotes	library
T3773	7700-7707	NNS	denotes	screens
T3775	7708-7710	IN	denotes	in
T3777	7711-7716	WDT	denotes	which
T3778	7717-7723	NN	denotes	TRIM11
T3779	7724-7727	VBD	denotes	was
T3776	7728-7736	VBN	denotes	isolated
T3780	7737-7741	IN	denotes	with
T3781	7742-7745	DT	denotes	the
T3783	7746-7749	NN	denotes	PST
T3782	7750-7756	NN	denotes	domain
T3784	7757-7760	CC	denotes	but
T3785	7761-7764	RB	denotes	not
T3786	7765-7769	IN	denotes	with
T3787	7770-7773	DT	denotes	the
T3789	7774-7775	NN	denotes	C
T3791	7775-7776	HYPH	denotes	-
T3790	7776-7784	JJ	denotes	terminal
T3788	7785-7792	NN	denotes	peptide
T3792	7793-7796	CC	denotes	and
T3793	7797-7805	VBZ	denotes	supports
T3794	7806-7809	DT	denotes	the
T3795	7810-7814	NN	denotes	idea
T3796	7815-7819	IN	denotes	that
T3798	7820-7823	DT	denotes	the
T3800	7824-7825	NN	denotes	C
T3802	7825-7826	HYPH	denotes	-
T3801	7826-7834	JJ	denotes	terminal
T3799	7835-7842	NN	denotes	peptide
T3797	7843-7845	VBZ	denotes	is
T3803	7846-7849	RB	denotes	not
T3804	7850-7859	JJ	denotes	important
T3805	7860-7863	IN	denotes	for
T3806	7864-7867	DT	denotes	the
T3807	7868-7879	NN	denotes	interaction
T3808	7880-7887	IN	denotes	between
T3809	7888-7892	NN	denotes	PAX6
T3810	7893-7896	CC	denotes	and
T3811	7897-7903	NN	denotes	TRIM11
T3812	7903-7904	.	denotes	.
T3813	7904-7905	sentence	denotes
T8632	7915-7919	NN	denotes	LacZ
T8634	7920-7928	NN	denotes	reporter
T8635	7929-7933	NN	denotes	gene
T8633	7934-7944	NN	denotes	activation
T8636	7945-7947	IN	denotes	in
T8637	7948-7956	JJ	denotes	pairwise
T8638	7957-7962	NNS	denotes	tests
T8639	7962-7963	.	denotes	.
T8640	7963-8064	sentence	denotes	pPC86 constructs are shown across the top, and pDBLeu constructs are shown down the right hand side.
T8641	7964-7969	NN	denotes	pPC86
T8642	7970-7980	NNS	denotes	constructs
T8644	7981-7984	VBP	denotes	are
T8643	7985-7990	VBN	denotes	shown
T8645	7991-7997	IN	denotes	across
T8646	7998-8001	DT	denotes	the
T8647	8002-8005	NN	denotes	top
T8648	8005-8007	,	denotes	,
T8649	8007-8010	CC	denotes	and
T8650	8011-8017	NN	denotes	pDBLeu
T8651	8018-8028	NNS	denotes	constructs
T8653	8029-8032	VBP	denotes	are
T8652	8033-8038	VBN	denotes	shown
T8654	8039-8043	IN	denotes	down
T8655	8044-8047	DT	denotes	the
T8657	8048-8053	JJ	denotes	right
T8658	8054-8058	NN	denotes	hand
T8656	8059-8063	NN	denotes	side
T8659	8063-8064	.	denotes	.
T8660	8064-8325	sentence	denotes	PAX6PST, PAX6 PST domain; PAX6PST/Q422R, PAX6 PST domain with the Q422R mutation; PAX6PST/X423L, PAX6 PST domain with the X423L mutation, PAX6PST/1615del10, PAX6 PST domain with the 1615 del10 mutation; PAX6PST-CT, PAX6 PST domain minus the C-terminal peptide.
T8661	8065-8072	NN	denotes	PAX6PST
T8662	8072-8074	,	denotes	,
T8663	8074-8078	NN	denotes	PAX6
T8665	8079-8082	NN	denotes	PST
T8664	8083-8089	NN	denotes	domain
T8666	8089-8090	:	denotes	;
T8667	8091-8098	NN	denotes	PAX6PST
T8669	8098-8099	HYPH	denotes	/
T8668	8099-8104	NN	denotes	Q422R
T8670	8104-8106	,	denotes	,
T8671	8106-8110	NN	denotes	PAX6
T8673	8111-8114	NN	denotes	PST
T8672	8115-8121	NN	denotes	domain
T8674	8122-8126	IN	denotes	with
T8675	8127-8130	DT	denotes	the
T8677	8131-8136	NN	denotes	Q422R
T8676	8137-8145	NN	denotes	mutation
T8678	8145-8146	:	denotes	;
T8679	8147-8154	NN	denotes	PAX6PST
T8681	8154-8155	HYPH	denotes	/
T8680	8155-8160	NN	denotes	X423L
T8682	8160-8162	,	denotes	,
T8683	8162-8166	NN	denotes	PAX6
T8685	8167-8170	NN	denotes	PST
T8684	8171-8177	NN	denotes	domain
T8686	8178-8182	IN	denotes	with
T8687	8183-8186	DT	denotes	the
T8689	8187-8192	NN	denotes	X423L
T8688	8193-8201	NN	denotes	mutation
T8690	8201-8203	,	denotes	,
T8691	8203-8210	NN	denotes	PAX6PST
T8693	8210-8211	HYPH	denotes	/
T8692	8211-8220	NN	denotes	1615del10
T8694	8220-8222	,	denotes	,
T8695	8222-8226	NN	denotes	PAX6
T8697	8227-8230	NN	denotes	PST
T8696	8231-8237	NN	denotes	domain
T8698	8238-8242	IN	denotes	with
T8699	8243-8246	DT	denotes	the
T8701	8247-8251	CD	denotes	1615
T8702	8252-8257	NN	denotes	del10
T8700	8258-8266	NN	denotes	mutation
T8703	8266-8267	:	denotes	;
T8704	8268-8275	NN	denotes	PAX6PST
T8706	8275-8276	HYPH	denotes	-
T8705	8276-8278	NN	denotes	CT
T8707	8278-8280	,	denotes	,
T8708	8280-8284	NN	denotes	PAX6
T8709	8285-8288	NN	denotes	PST
T8710	8289-8295	NN	denotes	domain
T8711	8296-8301	CC	denotes	minus
T8712	8302-8305	DT	denotes	the
T8714	8306-8307	NN	denotes	C
T8716	8307-8308	HYPH	denotes	-
T8715	8308-8316	JJ	denotes	terminal
T8713	8317-8324	NN	denotes	peptide
T8717	8324-8325	.	denotes	.
T8718	8325-8393	sentence	denotes	'Truncated HOMER3' is HOMER3 lacking the N-terminal 70 amino acids.
T8719	8326-8327	``	denotes	'
T8721	8327-8336	VBN	denotes	Truncated
T8722	8337-8343	NN	denotes	HOMER3
T8723	8343-8344	''	denotes	'
T8720	8345-8347	VBZ	denotes	is
T8724	8348-8354	NN	denotes	HOMER3
T8725	8355-8362	VBG	denotes	lacking
T8726	8363-8366	DT	denotes	the
T8728	8367-8368	NN	denotes	N
T8730	8368-8369	HYPH	denotes	-
T8729	8369-8377	JJ	denotes	terminal
T8731	8378-8380	CD	denotes	70
T8732	8381-8386	NN	denotes	amino
T8727	8387-8392	NNS	denotes	acids
T8733	8392-8393	.	denotes	.
T8734	8393-8447	sentence	denotes	Five control strains are shown for comparison (left).
T8735	8394-8398	CD	denotes	Five
T8737	8399-8406	NN	denotes	control
T8736	8407-8414	NNS	denotes	strains
T8739	8415-8418	VBP	denotes	are
T8738	8419-8424	VBN	denotes	shown
T8740	8425-8428	IN	denotes	for
T8741	8429-8439	NN	denotes	comparison
T8742	8440-8441	-LRB-	denotes	(
T8743	8441-8445	JJ	denotes	left
T8744	8445-8446	-RRB-	denotes	)
T8745	8446-8447	.	denotes	.
T8746	8447-8509	sentence	denotes	These range from non-interactor (A) to strong interactor (E).
T8747	8448-8453	DT	denotes	These
T8748	8454-8459	VBP	denotes	range
T8749	8460-8464	IN	denotes	from
T8750	8465-8479	NN	denotes	non-interactor
T8751	8480-8481	-LRB-	denotes	(
T8752	8481-8482	NN	denotes	A
T8753	8482-8483	-RRB-	denotes	)
T8754	8484-8486	IN	denotes	to
T8755	8487-8493	JJ	denotes	strong
T8756	8494-8504	NN	denotes	interactor
T8757	8505-8506	-LRB-	denotes	(
T8758	8506-8507	NN	denotes	E
T8759	8507-8508	-RRB-	denotes	)
T8760	8508-8509	.	denotes	.
T8837	8519-8527	JJ	denotes	Pairwise
T8839	8528-8539	NN	denotes	interaction
T8838	8540-8545	NNS	denotes	tests
T8840	8546-8553	IN	denotes	between
T8841	8554-8560	JJ	denotes	normal
T8843	8561-8564	CC	denotes	and
T8844	8565-8571	JJ	denotes	mutant
T8845	8572-8576	NN	denotes	PAX6
T8842	8577-8587	NNS	denotes	constructs
T8846	8588-8591	CC	denotes	and
T8847	8592-8598	NN	denotes	HOMER3
T8848	8598-8600	,	denotes	,
T8849	8600-8605	NN	denotes	DNCL1
T8850	8606-8609	CC	denotes	and
T8851	8610-8616	NN	denotes	TRIM11
T8852	8616-8617	SYM	denotes	.
T8853	8617-8791	sentence	denotes	+++: strong interaction; ++: moderate interaction; +: weak interaction; (+) borderline interaction with one or two reporters activated at very low levels; 0: no interaction.
T8854	8618-8621	SYM	denotes	+++
T8855	8621-8623	:	denotes	:
T8856	8623-8629	JJ	denotes	strong
T8857	8630-8641	NN	denotes	interaction
T8858	8641-8642	,	denotes	;
T8859	8643-8645	SYM	denotes	++
T8860	8645-8647	:	denotes	:
T8861	8647-8655	JJ	denotes	moderate
T8862	8656-8667	NN	denotes	interaction
T8863	8667-8668	,	denotes	;
T8864	8669-8670	SYM	denotes	+
T8865	8670-8672	:	denotes	:
T8866	8672-8676	JJ	denotes	weak
T8867	8677-8688	NN	denotes	interaction
T8868	8688-8689	,	denotes	;
T8869	8690-8691	-LRB-	denotes	(
T8871	8691-8692	SYM	denotes	+
T8870	8692-8693	-RRB-	denotes	)
T8872	8694-8704	JJ	denotes	borderline
T8873	8705-8716	NN	denotes	interaction
T8874	8717-8721	IN	denotes	with
T8875	8722-8725	CD	denotes	one
T8877	8726-8728	CC	denotes	or
T8878	8729-8732	CD	denotes	two
T8876	8733-8742	NNS	denotes	reporters
T8879	8743-8752	VBN	denotes	activated
T8880	8753-8755	IN	denotes	at
T8881	8756-8760	RB	denotes	very
T8882	8761-8764	JJ	denotes	low
T8883	8765-8771	NNS	denotes	levels
T8884	8771-8772	,	denotes	;
T8885	8773-8774	CD	denotes	0
T8886	8774-8776	:	denotes	:
T8887	8776-8778	DT	denotes	no
T8888	8779-8790	NN	denotes	interaction
T8889	8790-8791	.	denotes	.
T8890	8791-9084	sentence	denotes	PAX6PST, PAX6 PST domain; PAX6CTP, PAX6 C-terminal peptide; PAX6PST-CT, PAX6 PST domain minus the C-terminal peptide; PAX6PST/Q422R, PAX6 PST domain with the Q422R mutation; PAX6PST/X423L, PAX6 PST domain with the X423L mutation, PAX6PST/1615del, PAX6 PST domain with the 1615 del10 mutation.
T8891	8792-8799	NN	denotes	PAX6PST
T8892	8799-8801	,	denotes	,
T8893	8801-8805	NN	denotes	PAX6
T8895	8806-8809	NN	denotes	PST
T8894	8810-8816	NN	denotes	domain
T8896	8816-8817	,	denotes	;
T8897	8818-8825	NN	denotes	PAX6CTP
T8898	8825-8827	,	denotes	,
T8899	8827-8831	NN	denotes	PAX6
T8901	8832-8833	NN	denotes	C
T8903	8833-8834	HYPH	denotes	-
T8902	8834-8842	JJ	denotes	terminal
T8900	8843-8850	NN	denotes	peptide
T8904	8850-8851	,	denotes	;
T8905	8852-8859	NN	denotes	PAX6PST
T8907	8859-8860	HYPH	denotes	-
T8906	8860-8862	NN	denotes	CT
T8908	8862-8864	,	denotes	,
T8909	8864-8868	NN	denotes	PAX6
T8911	8869-8872	NN	denotes	PST
T8910	8873-8879	NN	denotes	domain
T8912	8880-8885	CC	denotes	minus
T8913	8886-8889	DT	denotes	the
T8915	8890-8891	NN	denotes	C
T8917	8891-8892	HYPH	denotes	-
T8916	8892-8900	JJ	denotes	terminal
T8914	8901-8908	NN	denotes	peptide
T8918	8908-8909	,	denotes	;
T8919	8910-8917	NN	denotes	PAX6PST
T8921	8917-8918	HYPH	denotes	/
T8920	8918-8923	NN	denotes	Q422R
T8922	8923-8925	,	denotes	,
T8923	8925-8929	NN	denotes	PAX6
T8925	8930-8933	NN	denotes	PST
T8924	8934-8940	NN	denotes	domain
T8926	8941-8945	IN	denotes	with
T8927	8946-8949	DT	denotes	the
T8929	8950-8955	NN	denotes	Q422R
T8928	8956-8964	NN	denotes	mutation
T8930	8964-8965	,	denotes	;
T8931	8966-8973	NN	denotes	PAX6PST
T8933	8973-8974	HYPH	denotes	/
T8932	8974-8979	NN	denotes	X423L
T8934	8979-8981	,	denotes	,
T8935	8981-8985	NN	denotes	PAX6
T8937	8986-8989	NN	denotes	PST
T8936	8990-8996	NN	denotes	domain
T8938	8997-9001	IN	denotes	with
T8939	9002-9005	DT	denotes	the
T8941	9006-9011	NN	denotes	X423L
T8940	9012-9020	NN	denotes	mutation
T8942	9020-9022	,	denotes	,
T8943	9022-9029	NN	denotes	PAX6PST
T8945	9029-9030	HYPH	denotes	/
T8944	9030-9037	NN	denotes	1615del
T8946	9037-9039	,	denotes	,
T8947	9039-9043	NN	denotes	PAX6
T8949	9044-9047	NN	denotes	PST
T8948	9048-9054	NN	denotes	domain
T8950	9055-9059	IN	denotes	with
T8951	9060-9063	DT	denotes	the
T8953	9064-9068	CD	denotes	1615
T8954	9069-9074	NN	denotes	del10
T8952	9075-9083	NN	denotes	mutation
T8955	9083-9084	.	denotes	.
T8956	9084-9194	sentence	denotes	HOMER3-FL, HOMER3 full-length clone; HOMER3-Tr, truncated HOMER3 clone lacking the N-terminal 70 amino acids.
T8957	9085-9091	NN	denotes	HOMER3
T8959	9091-9092	HYPH	denotes	-
T8958	9092-9094	NN	denotes	FL
T8960	9094-9096	,	denotes	,
T8961	9096-9102	NN	denotes	HOMER3
T8963	9103-9107	JJ	denotes	full
T8965	9107-9108	HYPH	denotes	-
T8964	9108-9114	NN	denotes	length
T8962	9115-9120	NN	denotes	clone
T8966	9120-9121	,	denotes	;
T8967	9122-9128	NN	denotes	HOMER3
T8969	9128-9129	HYPH	denotes	-
T8968	9129-9131	NN	denotes	Tr
T8970	9131-9133	,	denotes	,
T8971	9133-9142	VBN	denotes	truncated
T8973	9143-9149	NN	denotes	HOMER3
T8972	9150-9155	NN	denotes	clone
T8974	9156-9163	VBG	denotes	lacking
T8975	9164-9167	DT	denotes	the
T8977	9168-9169	NN	denotes	N
T8979	9169-9170	HYPH	denotes	-
T8978	9170-9178	JJ	denotes	terminal
T8980	9179-9181	CD	denotes	70
T8981	9182-9187	NN	denotes	amino
T8976	9188-9193	NNS	denotes	acids
T8982	9193-9194	.	denotes	.
T3815	9428-9429	NN	denotes	H
T3814	9428-9430	sentence	denotes	Ha
T3816	9429-9430	VBG	denotes	a
T3818	9430-9431	CD	denotes	v
T3817	9430-9433	sentence	denotes	vin
T3819	9431-9433	NNS	denotes	in
T3821	9433-9434	VBP	denotes	g
T3820	9433-9438	sentence	denotes	g con
T3822	9435-9437	NN	denotes	co
T3823	9437-9438	JJ	denotes	n
T3825	9438-9439	JJ	denotes	f
T3824	9438-9442	sentence	denotes	firm
T3826	9439-9440	VBG	denotes	i
T3827	9440-9442	JJ	denotes	rm
T3829	9442-9444	VBN	denotes	ed
T3828	9442-9671	sentence	denotes	ed that HOMER3, DNCL1 and TRIM11 interact with the PAX6 PST domain, we next investigated how the interactions were affected by three C-terminal PAX6 mutations that have been previously described in patients with ocular anomalies.
T3831	9445-9449	IN	denotes	that
T3833	9450-9456	NN	denotes	HOMER3
T3834	9456-9458	,	denotes	,
T3835	9458-9463	NN	denotes	DNCL1
T3836	9464-9467	CC	denotes	and
T3837	9468-9474	NN	denotes	TRIM11
T3832	9475-9483	VBP	denotes	interact
T3838	9484-9488	IN	denotes	with
T3839	9489-9492	DT	denotes	the
T3841	9493-9497	NN	denotes	PAX6
T3842	9498-9501	NN	denotes	PST
T3840	9502-9508	NN	denotes	domain
T3843	9508-9510	,	denotes	,
T3844	9510-9512	PRP	denotes	we
T3845	9513-9517	RB	denotes	next
T3830	9518-9530	VBD	denotes	investigated
T3846	9531-9534	WRB	denotes	how
T3848	9535-9538	DT	denotes	the
T3849	9539-9551	NNS	denotes	interactions
T3850	9552-9556	VBD	denotes	were
T3847	9557-9565	VBN	denotes	affected
T3851	9566-9568	IN	denotes	by
T3852	9569-9574	CD	denotes	three
T3854	9575-9576	NN	denotes	C
T3856	9576-9577	HYPH	denotes	-
T3855	9577-9585	JJ	denotes	terminal
T3857	9586-9590	NN	denotes	PAX6
T3853	9591-9600	NNS	denotes	mutations
T3858	9601-9605	WDT	denotes	that
T3860	9606-9610	VBP	denotes	have
T3861	9611-9615	VBN	denotes	been
T3862	9616-9626	RB	denotes	previously
T3859	9627-9636	VBN	denotes	described
T3863	9637-9639	IN	denotes	in
T3864	9640-9648	NNS	denotes	patients
T3865	9649-9653	IN	denotes	with
T3866	9654-9660	JJ	denotes	ocular
T3867	9661-9670	NNS	denotes	anomalies
T3868	9670-9671	.	denotes	.
T3869	9671-9821	sentence	denotes	The first mutation is a single nucleotide substitution 1627A>G that causes a glutamine to arginine amino acid substitution in the last codon of PAX6.
T3870	9672-9675	DT	denotes	The
T3872	9676-9681	JJ	denotes	first
T3871	9682-9690	NN	denotes	mutation
T3873	9691-9693	VBZ	denotes	is
T3874	9694-9695	DT	denotes	a
T3876	9696-9702	JJ	denotes	single
T3877	9703-9713	NN	denotes	nucleotide
T3875	9714-9726	NN	denotes	substitution
T3878	9727-9732	NN	denotes	1627A
T3879	9732-9733	SYM	denotes	>
T3880	9733-9734	NN	denotes	G
T3881	9735-9739	WDT	denotes	that
T3882	9740-9746	VBZ	denotes	causes
T3883	9747-9748	DT	denotes	a
T3885	9749-9758	NN	denotes	glutamine
T3886	9759-9761	IN	denotes	to
T3887	9762-9770	NN	denotes	arginine
T3888	9771-9776	NN	denotes	amino
T3889	9777-9781	NN	denotes	acid
T3884	9782-9794	NN	denotes	substitution
T3890	9795-9797	IN	denotes	in
T3891	9798-9801	DT	denotes	the
T3893	9802-9806	JJ	denotes	last
T3892	9807-9812	NN	denotes	codon
T3894	9813-9815	IN	denotes	of
T3895	9816-9820	NN	denotes	PAX6
T3896	9820-9821	.	denotes	.
T3897	9821-10013	sentence	denotes	This missense mutation (Q422R) has been reported in two patients, one affected by anterior segment dysgenesis with uveal ectropion and one with typical aniridia and foveal hypoplasia [27,33].
T3898	9822-9826	DT	denotes	This
T3900	9827-9835	JJ	denotes	missense
T3899	9836-9844	NN	denotes	mutation
T3902	9845-9846	-LRB-	denotes	(
T3903	9846-9851	NN	denotes	Q422R
T3904	9851-9852	-RRB-	denotes	)
T3905	9853-9856	VBZ	denotes	has
T3906	9857-9861	VBN	denotes	been
T3901	9862-9870	VBN	denotes	reported
T3907	9871-9873	IN	denotes	in
T3908	9874-9877	CD	denotes	two
T3909	9878-9886	NNS	denotes	patients
T3910	9886-9888	,	denotes	,
T3911	9888-9891	CD	denotes	one
T3912	9892-9900	VBN	denotes	affected
T3913	9901-9903	IN	denotes	by
T3914	9904-9912	JJ	denotes	anterior
T3916	9913-9920	NN	denotes	segment
T3915	9921-9931	NN	denotes	dysgenesis
T3917	9932-9936	IN	denotes	with
T3918	9937-9942	JJ	denotes	uveal
T3919	9943-9952	NN	denotes	ectropion
T3920	9953-9956	CC	denotes	and
T3921	9957-9960	CD	denotes	one
T3922	9961-9965	IN	denotes	with
T3923	9966-9973	JJ	denotes	typical
T3924	9974-9982	NN	denotes	aniridia
T3925	9983-9986	CC	denotes	and
T3926	9987-9993	JJ	denotes	foveal
T3927	9994-10004	NN	denotes	hypoplasia
T3928	10005-10006	-LRB-	denotes	[
T3930	10006-10008	CD	denotes	27
T3931	10008-10009	,	denotes	,
T3929	10009-10011	CD	denotes	33
T3932	10011-10012	-RRB-	denotes	]
T3933	10012-10013	.	denotes	.
T3934	10013-10083	sentence	denotes	The second mutation (1615del10) was found in an aniridia family [28].
T3935	10014-10017	DT	denotes	The
T3937	10018-10024	JJ	denotes	second
T3936	10025-10033	NN	denotes	mutation
T3939	10034-10035	-LRB-	denotes	(
T3940	10035-10044	NN	denotes	1615del10
T3941	10044-10045	-RRB-	denotes	)
T3942	10046-10049	VBD	denotes	was
T3938	10050-10055	VBN	denotes	found
T3943	10056-10058	IN	denotes	in
T3944	10059-10061	DT	denotes	an
T3946	10062-10070	NN	denotes	aniridia
T3945	10071-10077	NN	denotes	family
T3947	10078-10079	-LRB-	denotes	[
T3948	10079-10081	CD	denotes	28
T3949	10081-10082	-RRB-	denotes	]
T3950	10082-10083	.	denotes	.
T3951	10083-10360	sentence	denotes	This frame-shifting deletion occurs just before the PAX6 stop codon and is predicted to cause translational read-through into the 3' untranslated region, generating a protein in which the last 5 amino acids of the C-terminal peptide are replaced by a 103 amino acid-extension.
T3952	10084-10088	DT	denotes	This
T3954	10089-10094	NN	denotes	frame
T3956	10094-10095	HYPH	denotes	-
T3955	10095-10103	VBG	denotes	shifting
T3953	10104-10112	NN	denotes	deletion
T3957	10113-10119	VBZ	denotes	occurs
T3958	10120-10124	RB	denotes	just
T3959	10125-10131	IN	denotes	before
T3960	10132-10135	DT	denotes	the
T3962	10136-10140	NN	denotes	PAX6
T3963	10141-10145	NN	denotes	stop
T3961	10146-10151	NN	denotes	codon
T3964	10152-10155	CC	denotes	and
T3965	10156-10158	VBZ	denotes	is
T3966	10159-10168	VBN	denotes	predicted
T3967	10169-10171	TO	denotes	to
T3968	10172-10177	VB	denotes	cause
T3969	10178-10191	JJ	denotes	translational
T3971	10192-10196	NN	denotes	read
T3972	10196-10197	HYPH	denotes	-
T3970	10197-10204	NN	denotes	through
T3973	10205-10209	IN	denotes	into
T3974	10210-10213	DT	denotes	the
T3976	10214-10215	CD	denotes	3
T3977	10215-10216	SYM	denotes	'
T3978	10217-10229	JJ	denotes	untranslated
T3975	10230-10236	NN	denotes	region
T3979	10236-10238	,	denotes	,
T3980	10238-10248	VBG	denotes	generating
T3981	10249-10250	DT	denotes	a
T3982	10251-10258	NN	denotes	protein
T3983	10259-10261	IN	denotes	in
T3985	10262-10267	WDT	denotes	which
T3986	10268-10271	DT	denotes	the
T3988	10272-10276	JJ	denotes	last
T3989	10277-10278	CD	denotes	5
T3990	10279-10284	NN	denotes	amino
T3987	10285-10290	NNS	denotes	acids
T3991	10291-10293	IN	denotes	of
T3992	10294-10297	DT	denotes	the
T3994	10298-10299	NN	denotes	C
T3996	10299-10300	HYPH	denotes	-
T3995	10300-10308	JJ	denotes	terminal
T3993	10309-10316	NN	denotes	peptide
T3997	10317-10320	VBP	denotes	are
T3984	10321-10329	VBN	denotes	replaced
T3998	10330-10332	IN	denotes	by
T3999	10333-10334	DT	denotes	a
T4001	10335-10338	CD	denotes	103
T4003	10339-10344	NN	denotes	amino
T4002	10345-10349	NN	denotes	acid
T4004	10349-10350	HYPH	denotes	-
T4000	10350-10359	NN	denotes	extension
T4005	10359-10360	.	denotes	.
T4006	10360-10504	sentence	denotes	Affected individuals in this family showed unusual neurobehavioural traits including impaired social cognition and poor verbal inhibition [28].
T4007	10361-10369	VBN	denotes	Affected
T4008	10370-10381	NNS	denotes	individuals
T4010	10382-10384	IN	denotes	in
T4011	10385-10389	DT	denotes	this
T4012	10390-10396	NN	denotes	family
T4009	10397-10403	VBD	denotes	showed
T4013	10404-10411	JJ	denotes	unusual
T4015	10412-10428	JJ	denotes	neurobehavioural
T4014	10429-10435	NNS	denotes	traits
T4016	10436-10445	VBG	denotes	including
T4017	10446-10454	VBN	denotes	impaired
T4019	10455-10461	JJ	denotes	social
T4018	10462-10471	NN	denotes	cognition
T4020	10472-10475	CC	denotes	and
T4021	10476-10480	JJ	denotes	poor
T4023	10481-10487	JJ	denotes	verbal
T4022	10488-10498	NN	denotes	inhibition
T4024	10499-10500	-LRB-	denotes	[
T4025	10500-10502	CD	denotes	28
T4026	10502-10503	-RRB-	denotes	]
T4027	10503-10504	.	denotes	.
T4028	10504-10658	sentence	denotes	MRI analysis revealed grey matter abnormalities in the frontal lobe, temporal lobe and cerebellum, and white matter deficits in the corpus callosum [13].
T4029	10505-10508	NN	denotes	MRI
T4030	10509-10517	NN	denotes	analysis
T4031	10518-10526	VBD	denotes	revealed
T4032	10527-10531	JJ	denotes	grey
T4033	10532-10538	NN	denotes	matter
T4034	10539-10552	NNS	denotes	abnormalities
T4035	10553-10555	IN	denotes	in
T4036	10556-10559	DT	denotes	the
T4038	10560-10567	JJ	denotes	frontal
T4037	10568-10572	NN	denotes	lobe
T4039	10572-10574	,	denotes	,
T4040	10574-10582	JJ	denotes	temporal
T4041	10583-10587	NN	denotes	lobe
T4042	10588-10591	CC	denotes	and
T4043	10592-10602	NN	denotes	cerebellum
T4044	10602-10604	,	denotes	,
T4045	10604-10607	CC	denotes	and
T4046	10608-10613	JJ	denotes	white
T4047	10614-10620	NN	denotes	matter
T4048	10621-10629	NNS	denotes	deficits
T4049	10630-10632	IN	denotes	in
T4050	10633-10636	DT	denotes	the
T4052	10637-10643	NN	denotes	corpus
T4051	10644-10652	NN	denotes	callosum
T4053	10653-10654	-LRB-	denotes	[
T4054	10654-10656	CD	denotes	13
T4055	10656-10657	-RRB-	denotes	]
T4056	10657-10658	.	denotes	.
T4057	10658-10756	sentence	denotes	The third mutation 1629insT (X423L) has been reported in several aniridia patients [11,12,27,33].
T4058	10659-10662	DT	denotes	The
T4060	10663-10668	JJ	denotes	third
T4059	10669-10677	NN	denotes	mutation
T4062	10678-10686	NN	denotes	1629insT
T4063	10687-10688	-LRB-	denotes	(
T4064	10688-10693	NN	denotes	X423L
T4065	10693-10694	-RRB-	denotes	)
T4066	10695-10698	VBZ	denotes	has
T4067	10699-10703	VBN	denotes	been
T4061	10704-10712	VBN	denotes	reported
T4068	10713-10715	IN	denotes	in
T4069	10716-10723	JJ	denotes	several
T4071	10724-10732	NN	denotes	aniridia
T4070	10733-10741	NNS	denotes	patients
T4072	10742-10743	-LRB-	denotes	[
T4074	10743-10745	CD	denotes	11
T4075	10745-10746	,	denotes	,
T4076	10746-10748	CD	denotes	12
T4077	10748-10749	,	denotes	,
T4078	10749-10751	CD	denotes	27
T4079	10751-10752	,	denotes	,
T4073	10752-10754	CD	denotes	33
T4080	10754-10755	-RRB-	denotes	]
T4081	10755-10756	.	denotes	.
T4082	10756-11006	sentence	denotes	Insertion of a single T nucleotide at position 1629 changes the stop codon (TAA) to a leucine codon (TTA) and generates a full length PAX6 protein with a C-terminal extension that extends for a further 35 amino acids into the 3' untranslated region.
T4083	10757-10766	NN	denotes	Insertion
T4085	10767-10769	IN	denotes	of
T4086	10770-10771	DT	denotes	a
T4088	10772-10778	JJ	denotes	single
T4089	10779-10780	NN	denotes	T
T4087	10781-10791	NN	denotes	nucleotide
T4090	10792-10794	IN	denotes	at
T4091	10795-10803	NN	denotes	position
T4092	10804-10808	CD	denotes	1629
T4084	10809-10816	VBZ	denotes	changes
T4093	10817-10820	DT	denotes	the
T4095	10821-10825	NN	denotes	stop
T4094	10826-10831	NN	denotes	codon
T4096	10832-10833	-LRB-	denotes	(
T4097	10833-10836	NN	denotes	TAA
T4098	10836-10837	-RRB-	denotes	)
T4099	10838-10840	IN	denotes	to
T4100	10841-10842	DT	denotes	a
T4102	10843-10850	NN	denotes	leucine
T4101	10851-10856	NN	denotes	codon
T4103	10857-10858	-LRB-	denotes	(
T4104	10858-10861	NN	denotes	TTA
T4105	10861-10862	-RRB-	denotes	)
T4106	10863-10866	CC	denotes	and
T4107	10867-10876	VBZ	denotes	generates
T4108	10877-10878	DT	denotes	a
T4110	10879-10883	JJ	denotes	full
T4111	10884-10890	NN	denotes	length
T4112	10891-10895	NN	denotes	PAX6
T4109	10896-10903	NN	denotes	protein
T4113	10904-10908	IN	denotes	with
T4114	10909-10910	DT	denotes	a
T4116	10911-10912	NN	denotes	C
T4118	10912-10913	HYPH	denotes	-
T4117	10913-10921	JJ	denotes	terminal
T4115	10922-10931	NN	denotes	extension
T4119	10932-10936	WDT	denotes	that
T4120	10937-10944	VBZ	denotes	extends
T4121	10945-10948	IN	denotes	for
T4122	10949-10950	DT	denotes	a
T4124	10951-10958	JJ	denotes	further
T4125	10959-10961	CD	denotes	35
T4126	10962-10967	NN	denotes	amino
T4123	10968-10973	NNS	denotes	acids
T4127	10974-10978	IN	denotes	into
T4128	10979-10982	DT	denotes	the
T4130	10983-10984	CD	denotes	3
T4131	10984-10985	SYM	denotes	'
T4132	10986-10998	JJ	denotes	untranslated
T4129	10999-11005	NN	denotes	region
T4133	11005-11006	.	denotes	.
T4134	11006-11185	sentence	denotes	MRI analysis of six patients with this mutation revealed variable brain defects including absence or hypoplasia of the anterior commissure, pineal gland and olfactory bulbs [12].
T4135	11007-11010	NN	denotes	MRI
T4136	11011-11019	NN	denotes	analysis
T4138	11020-11022	IN	denotes	of
T4139	11023-11026	CD	denotes	six
T4140	11027-11035	NNS	denotes	patients
T4141	11036-11040	IN	denotes	with
T4142	11041-11045	DT	denotes	this
T4143	11046-11054	NN	denotes	mutation
T4137	11055-11063	VBD	denotes	revealed
T4144	11064-11072	JJ	denotes	variable
T4146	11073-11078	NN	denotes	brain
T4145	11079-11086	NNS	denotes	defects
T4147	11087-11096	VBG	denotes	including
T4148	11097-11104	NN	denotes	absence
T4149	11105-11107	CC	denotes	or
T4150	11108-11118	NN	denotes	hypoplasia
T4151	11119-11121	IN	denotes	of
T4152	11122-11125	DT	denotes	the
T4154	11126-11134	JJ	denotes	anterior
T4153	11135-11145	NN	denotes	commissure
T4155	11145-11147	,	denotes	,
T4156	11147-11153	JJ	denotes	pineal
T4157	11154-11159	NN	denotes	gland
T4158	11160-11163	CC	denotes	and
T4159	11164-11173	JJ	denotes	olfactory
T4160	11174-11179	NNS	denotes	bulbs
T4161	11180-11181	-LRB-	denotes	[
T4162	11181-11183	CD	denotes	12
T4163	11183-11184	-RRB-	denotes	]
T4164	11184-11185	.	denotes	.
T4165	11185-11266	sentence	denotes	Two patients had temporal polymicrogyria, one in association with epilepsy [12].
T4166	11186-11189	CD	denotes	Two
T4167	11190-11198	NNS	denotes	patients
T4168	11199-11202	VBD	denotes	had
T4170	11203-11211	JJ	denotes	temporal
T4171	11212-11226	NN	denotes	polymicrogyria
T4172	11226-11228	,	denotes	,
T4169	11228-11231	CD	denotes	one
T4173	11232-11234	IN	denotes	in
T4174	11235-11246	NN	denotes	association
T4175	11247-11251	IN	denotes	with
T4176	11252-11260	NN	denotes	epilepsy
T4177	11261-11262	-LRB-	denotes	[
T4178	11262-11264	CD	denotes	12
T4179	11264-11265	-RRB-	denotes	]
T4180	11265-11266	.	denotes	.
T4181	11266-11451	sentence	denotes	The three mutations were introduced into the PAX6PST construct, and pairwise tests were carried out to investigate the interaction of each mutant protein with HOMER3, DNCL1 and TRIM11.
T4182	11267-11270	DT	denotes	The
T4184	11271-11276	CD	denotes	three
T4183	11277-11286	NNS	denotes	mutations
T4186	11287-11291	VBD	denotes	were
T4185	11292-11302	VBN	denotes	introduced
T4187	11303-11307	IN	denotes	into
T4188	11308-11311	DT	denotes	the
T4190	11312-11319	NN	denotes	PAX6PST
T4189	11320-11329	NN	denotes	construct
T4191	11329-11331	,	denotes	,
T4192	11331-11334	CC	denotes	and
T4193	11335-11343	JJ	denotes	pairwise
T4194	11344-11349	NNS	denotes	tests
T4196	11350-11354	VBD	denotes	were
T4195	11355-11362	VBN	denotes	carried
T4197	11363-11366	RP	denotes	out
T4198	11367-11369	TO	denotes	to
T4199	11370-11381	VB	denotes	investigate
T4200	11382-11385	DT	denotes	the
T4201	11386-11397	NN	denotes	interaction
T4202	11398-11400	IN	denotes	of
T4203	11401-11405	DT	denotes	each
T4205	11406-11412	NN	denotes	mutant
T4204	11413-11420	NN	denotes	protein
T4206	11421-11425	IN	denotes	with
T4207	11426-11432	NN	denotes	HOMER3
T4208	11432-11434	,	denotes	,
T4209	11434-11439	NN	denotes	DNCL1
T4210	11440-11443	CC	denotes	and
T4211	11444-11450	NN	denotes	TRIM11
T4212	11450-11451	.	denotes	.
T4213	11451-11511	sentence	denotes	All three mutations had a clear effect on the interactions.
T4214	11452-11455	DT	denotes	All
T4216	11456-11461	CD	denotes	three
T4215	11462-11471	NNS	denotes	mutations
T4217	11472-11475	VBD	denotes	had
T4218	11476-11477	DT	denotes	a
T4220	11478-11483	JJ	denotes	clear
T4219	11484-11490	NN	denotes	effect
T4221	11491-11493	IN	denotes	on
T4222	11494-11497	DT	denotes	the
T4223	11498-11510	NNS	denotes	interactions
T4224	11510-11511	.	denotes	.
T4225	11511-11625	sentence	denotes	The most subtle mutation (Q422R) caused a reduction in the interaction with HOMER3 and DNCL1 (Figure 3, Table 1).
T4226	11512-11515	DT	denotes	The
T4228	11516-11520	RBS	denotes	most
T4229	11521-11527	JJ	denotes	subtle
T4227	11528-11536	NN	denotes	mutation
T4231	11537-11538	-LRB-	denotes	(
T4232	11538-11543	NN	denotes	Q422R
T4233	11543-11544	-RRB-	denotes	)
T4230	11545-11551	VBD	denotes	caused
T4234	11552-11553	DT	denotes	a
T4235	11554-11563	NN	denotes	reduction
T4236	11564-11566	IN	denotes	in
T4237	11567-11570	DT	denotes	the
T4238	11571-11582	NN	denotes	interaction
T4239	11583-11587	IN	denotes	with
T4240	11588-11594	NN	denotes	HOMER3
T4241	11595-11598	CC	denotes	and
T4242	11599-11604	NN	denotes	DNCL1
T4243	11605-11606	-LRB-	denotes	(
T4245	11606-11612	NN	denotes	Figure
T4246	11613-11614	CD	denotes	3
T4247	11614-11616	,	denotes	,
T4244	11616-11621	NN	denotes	Table
T4248	11622-11623	CD	denotes	1
T4249	11623-11624	-RRB-	denotes	)
T4250	11624-11625	.	denotes	.
T4251	11625-11787	sentence	denotes	The C-terminal extension mutations X423L and1615del10 mutations both dramatically reduced or abolished the interaction with HOMER3 and DNCL1 (Figure 3, Table 1).
T4252	11626-11629	DT	denotes	The
T4254	11630-11631	NN	denotes	C
T4256	11631-11632	HYPH	denotes	-
T4255	11632-11640	JJ	denotes	terminal
T4257	11641-11650	NN	denotes	extension
T4258	11651-11660	NNS	denotes	mutations
T4253	11661-11666	NN	denotes	X423L
T4260	11667-11670	CC	denotes	and
T4261	11670-11679	NN	denotes	1615del10
T4262	11680-11689	NNS	denotes	mutations
T4263	11690-11694	DT	denotes	both
T4264	11695-11707	RB	denotes	dramatically
T4259	11708-11715	VBD	denotes	reduced
T4265	11716-11718	CC	denotes	or
T4266	11719-11728	VBD	denotes	abolished
T4267	11729-11732	DT	denotes	the
T4268	11733-11744	NN	denotes	interaction
T4269	11745-11749	IN	denotes	with
T4270	11750-11756	NN	denotes	HOMER3
T4271	11757-11760	CC	denotes	and
T4272	11761-11766	NN	denotes	DNCL1
T4273	11767-11768	-LRB-	denotes	(
T4275	11768-11774	NN	denotes	Figure
T4276	11775-11776	CD	denotes	3
T4277	11776-11778	,	denotes	,
T4274	11778-11783	NN	denotes	Table
T4278	11784-11785	CD	denotes	1
T4279	11785-11786	-RRB-	denotes	)
T4280	11786-11787	.	denotes	.
T4281	11787-11967	sentence	denotes	None of the three mutations affected the interaction with TRIM11 which again is consistent with the hypothesis that TRIM11 interacts with a more N-terminal part of the PST domain.
T4282	11788-11792	NN	denotes	None
T4284	11793-11795	IN	denotes	of
T4285	11796-11799	DT	denotes	the
T4287	11800-11805	CD	denotes	three
T4286	11806-11815	NNS	denotes	mutations
T4283	11816-11824	VBD	denotes	affected
T4288	11825-11828	DT	denotes	the
T4289	11829-11840	NN	denotes	interaction
T4290	11841-11845	IN	denotes	with
T4291	11846-11852	NN	denotes	TRIM11
T4292	11853-11858	WDT	denotes	which
T4294	11859-11864	RB	denotes	again
T4293	11865-11867	VBZ	denotes	is
T4295	11868-11878	JJ	denotes	consistent
T4296	11879-11883	IN	denotes	with
T4297	11884-11887	DT	denotes	the
T4298	11888-11898	NN	denotes	hypothesis
T4299	11899-11903	IN	denotes	that
T4301	11904-11910	NN	denotes	TRIM11
T4300	11911-11920	VBZ	denotes	interacts
T4302	11921-11925	IN	denotes	with
T4303	11926-11927	DT	denotes	a
T4305	11928-11932	RBR	denotes	more
T4307	11933-11934	NN	denotes	N
T4308	11934-11935	HYPH	denotes	-
T4306	11935-11943	JJ	denotes	terminal
T4304	11944-11948	NN	denotes	part
T4309	11949-11951	IN	denotes	of
T4310	11952-11955	DT	denotes	the
T4312	11956-11959	NN	denotes	PST
T4311	11960-11966	NN	denotes	domain
T4313	11966-11967	.	denotes	.
R1020	T1765	T1766	det	A,peptide
R1021	T1767	T1768	advmod	highly,conserved
R1022	T1768	T1766	amod	conserved,peptide
R1023	T1769	T1770	npadvmod	C,terminal
R1024	T1770	T1766	amod	terminal,peptide
R1025	T1771	T1770	punct	-,terminal
R1026	T1772	T1766	compound	PAX6,peptide
R1027	T1774	T1775	nsubj	We,noted
R1028	T1776	T1774	cc	and,We
R1029	T1777	T1774	conj	others,We
R1030	T1778	T1779	punct	[,31
R1031	T1779	T1777	parataxis	31,others
R1032	T1780	T1781	punct	-,33
R1033	T1781	T1779	prep	33,31
R1034	T1782	T1779	punct	],31
R1035	T1783	T1784	mark	that,is
R1036	T1784	T1775	ccomp	is,noted
R1037	T1785	T1784	expl	there,is
R1038	T1786	T1787	amod	significant,conservation
R1039	T1787	T1784	attr	conservation,is
R1040	T1788	T1787	compound	sequence,conservation
R1041	T1789	T1784	prep	at,is
R1042	T1790	T1791	det	the,end
R1043	T1791	T1789	pobj	end,at
R1044	T1792	T1793	npadvmod	C,terminal
R1045	T1793	T1791	amod	terminal,end
R1046	T1794	T1793	punct	-,terminal
R1047	T1795	T1791	prep	of,end
R1048	T1796	T1797	det	the,protein
R1049	T1797	T1795	pobj	protein,of
R1050	T1798	T1797	compound	PAX6,protein
R1051	T1799	T1775	punct	.,noted
R1052	T1801	T1802	compound	BLAST,analysis
R1053	T1802	T1803	nsubj	analysis,revealed
R1054	T1804	T1802	prep	of,analysis
R1055	T1805	T1806	det	the,sequence
R1056	T1806	T1804	pobj	sequence,of
R1057	T1807	T1808	compound	amino,acid
R1058	T1808	T1806	compound	acid,sequence
R1059	T1809	T1806	prep	of,sequence
R1060	T1810	T1811	det	the,domain
R1061	T1811	T1809	pobj	domain,of
R1062	T1812	T1811	compound	PAX6,domain
R1063	T1813	T1811	compound	PST,domain
R1064	T1814	T1815	punct	(,278
R1065	T1815	T1806	parataxis	278,sequence
R1066	T1816	T1815	nmod	aa,278
R1067	T1817	T1818	punct	–,422
R1068	T1818	T1815	prep	422,278
R1069	T1819	T1815	punct	),278
R1070	T1820	T1821	det	a,motif
R1071	T1821	T1803	dobj	motif,revealed
R1072	T1822	T1823	advmod	highly,conserved
R1073	T1823	T1821	amod	conserved,motif
R1074	T1824	T1821	prep	within,motif
R1075	T1825	T1826	det	the,acids
R1076	T1826	T1824	pobj	acids,within
R1077	T1827	T1826	amod	last,acids
R1078	T1828	T1826	nummod	28,acids
R1079	T1829	T1826	compound	amino,acids
R1080	T1830	T1821	punct	(,motif
R1081	T1831	T1821	acl	beginning,motif
R1082	T1832	T1831	prep	at,beginning
R1083	T1833	T1834	det	the,motif
R1084	T1834	T1832	pobj	motif,at
R1085	T1835	T1834	punct	',motif
R1086	T1836	T1834	nmod	GLISP,motif
R1087	T1837	T1834	punct	',motif
R1088	T1838	T1839	punct	", ",Figure
R1089	T1839	T1803	parataxis	Figure,revealed
R1090	T1840	T1839	nummod	1a,Figure
R1091	T1841	T1839	punct	),Figure
R1092	T1842	T1803	punct	.,revealed
R1093	T1844	T1845	amod	Strong,conservation
R1094	T1845	T1846	nsubjpass	conservation,seen
R1095	T1847	T1846	auxpass	was,seen
R1096	T1848	T1846	prep	in,seen
R1097	T1849	T1850	advmod	distantly,related
R1098	T1850	T1851	amod	related,species
R1099	T1851	T1848	pobj	species,in
R1100	T1852	T1853	amod	such,as
R1101	T1853	T1851	prep	as,species
R1102	T1854	T1853	pobj	axolotl,as
R1103	T1855	T1854	punct	(,axolotl
R1104	T1856	T1857	compound	Ambystoma,mexicanum
R1105	T1857	T1854	appos	mexicanum,axolotl
R1106	T1858	T1854	punct	),axolotl
R1107	T1859	T1854	cc	and,axolotl
R1108	T1860	T1861	compound	sea,urchin
R1109	T1861	T1854	conj	urchin,axolotl
R1110	T1862	T1861	punct	(,urchin
R1111	T1863	T1864	compound	Paracentrotus,lividus
R1112	T1864	T1861	appos	lividus,urchin
R1113	T1865	T1846	punct	),seen
R1114	T1866	T1867	punct	(,Figure
R1115	T1867	T1846	parataxis	Figure,seen
R1116	T1868	T1867	nummod	1a,Figure
R1117	T1869	T1867	punct	),Figure
R1118	T1870	T1846	punct	.,seen
R1119	T1872	T1873	nsubj	We,subjected
R1120	T1874	T1875	det	the,domain
R1121	T1875	T1873	dobj	domain,subjected
R1122	T1876	T1875	amod	whole,domain
R1123	T1877	T1875	compound	PAX6,domain
R1124	T1878	T1875	compound	PST,domain
R1125	T1879	T1873	prep	to,subjected
R1126	T1880	T1881	amod	secondary,analysis
R1127	T1881	T1879	pobj	analysis,to
R1128	T1882	T1881	compound	structure,analysis
R1129	T1883	T1873	advcl	using,subjected
R1130	T1884	T1883	dobj	JPRED,using
R1131	T1885	T1884	punct	", ",JPRED
R1132	T1886	T1887	det	a,program
R1133	T1887	T1884	appos	program,JPRED
R1134	T1888	T1889	dep	that,uses
R1135	T1889	T1887	relcl	uses,program
R1136	T1890	T1891	det	a,number
R1137	T1891	T1889	dobj	number,uses
R1138	T1892	T1891	prep	of,number
R1139	T1893	T1894	amod	different,algorithms
R1140	T1894	T1892	pobj	algorithms,of
R1141	T1895	T1896	compound	protein,structure
R1142	T1896	T1897	compound	structure,prediction
R1143	T1897	T1894	compound	prediction,algorithms
R1144	T1898	T1899	aux	to,generate
R1145	T1899	T1889	advcl	generate,uses
R1146	T1900	T1901	det	a,structure
R1147	T1901	T1899	dobj	structure,generate
R1148	T1902	T1901	nmod	consensus,structure
R1149	T1903	T1901	amod	secondary,structure
R1150	T1904	T1905	punct	(,Figure
R1151	T1905	T1873	parataxis	Figure,subjected
R1152	T1906	T1905	nummod	1,Figure
R1153	T1907	T1905	punct	),Figure
R1154	T1908	T1909	punct	[,26
R1155	T1909	T1873	parataxis	26,subjected
R1156	T1910	T1909	nummod	25,26
R1157	T1911	T1909	punct	",",26
R1158	T1912	T1909	punct	],26
R1159	T1913	T1873	punct	.,subjected
R1160	T1915	T1916	det	The,domain
R1161	T1916	T1918	nsubj	domain,was
R1162	T1917	T1916	compound	PST,domain
R1163	T1919	T1918	advmod	largely,was
R1164	T1920	T1918	acomp	devoid,was
R1165	T1921	T1920	prep	of,devoid
R1166	T1922	T1923	amod	predicted,structure
R1167	T1923	T1921	pobj	structure,of
R1168	T1924	T1923	amod	secondary,structure
R1169	T1925	T1918	prep	except,was
R1170	T1926	T1925	prep	for,except
R1171	T1927	T1928	det	the,region
R1172	T1928	T1926	pobj	region,for
R1173	T1929	T1930	npadvmod	C,terminal
R1174	T1930	T1928	amod	terminal,region
R1175	T1931	T1930	punct	-,terminal
R1176	T1932	T1928	punct	", ",region
R1177	T1933	T1934	dep	which,contained
R1178	T1934	T1928	relcl	contained,region
R1179	T1935	T1936	nummod	two,sheets
R1180	T1936	T1934	dobj	sheets,contained
R1181	T1937	T1936	amod	predicted,sheets
R1182	T1938	T1936	compound	beta,sheets
R1183	T1939	T1936	prep	within,sheets
R1184	T1940	T1941	det	the,domain
R1185	T1941	T1939	pobj	domain,within
R1186	T1942	T1941	advmod	highly,domain
R1187	T1943	T1941	amod	conserved,domain
R1188	T1944	T1936	punct	", ",sheets
R1189	T1945	T1936	appos	one,sheets
R1190	T1946	T1945	prep	in,one
R1191	T1947	T1948	det	the,GLISP
R1192	T1948	T1950	nmod	GLISP,motif
R1193	T1949	T1948	punct	',GLISP
R1194	T1950	T1946	pobj	motif,in
R1195	T1951	T1950	punct	',motif
R1196	T1952	T1945	cc	and,one
R1197	T1953	T1945	conj	one,one
R1198	T1954	T1953	prep	in,one
R1199	T1955	T1956	det	the,SPVPQ
R1200	T1956	T1958	nmod	SPVPQ,motif
R1201	T1957	T1956	punct	',SPVPQ
R1202	T1958	T1954	pobj	motif,in
R1203	T1959	T1958	punct	',motif
R1204	T1960	T1961	punct	(,identical
R1205	T1961	T1918	parataxis	identical,was
R1206	T1962	T1961	prep	to,identical
R1207	T1963	T1964	det	the,pattern
R1208	T1964	T1962	pobj	pattern,to
R1209	T1965	T1964	acl	shown,pattern
R1210	T1966	T1965	prep	in,shown
R1211	T1967	T1966	pobj	Figure,in
R1212	T1968	T1967	nummod	1c,Figure
R1213	T1969	T1961	punct	),identical
R1214	T1970	T1918	punct	.,was
R1215	T1972	T1973	advmod	Initially,defined
R1216	T1974	T1973	nsubj	we,defined
R1217	T1975	T1976	det	the,domain
R1218	T1976	T1973	dobj	domain,defined
R1219	T1977	T1978	npadvmod	C,terminal
R1220	T1978	T1976	amod	terminal,domain
R1221	T1979	T1978	punct	-,terminal
R1222	T1980	T1973	prep	as,defined
R1223	T1981	T1980	pcomp	running,as
R1224	T1982	T1981	prep	from,running
R1225	T1983	T1984	det	the,GLISP
R1226	T1984	T1986	nmod	GLISP,motif
R1227	T1985	T1984	punct	',GLISP
R1228	T1986	T1982	pobj	motif,from
R1229	T1987	T1984	punct	',GLISP
R1230	T1988	T1981	prep	up,running
R1231	T1989	T1988	prep	to,up
R1232	T1990	T1991	det	the,codon
R1233	T1991	T1989	pobj	codon,to
R1234	T1992	T1991	compound	stop,codon
R1235	T1993	T1973	punct	", ",defined
R1236	T1994	T1995	mark	since,conserved
R1237	T1995	T1973	advcl	conserved,defined
R1238	T1996	T1997	det	this,region
R1239	T1997	T1995	nsubjpass	region,conserved
R1240	T1998	T1995	auxpass	was,conserved
R1241	T1999	T2000	advmod	most,highly
R1242	T2000	T1995	advmod	highly,conserved
R1243	T2001	T1995	cc	and,conserved
R1244	T2002	T1995	conj	contained,conserved
R1245	T2003	T2004	advmod	strongly,predicted
R1246	T2004	T2005	amod	predicted,elements
R1247	T2005	T2002	dobj	elements,contained
R1248	T2006	T2007	amod	secondary,structure
R1249	T2007	T2005	compound	structure,elements
R1250	T2008	T1973	punct	.,defined
R1251	T2010	T2011	advmod	However,lost
R1252	T2012	T2013	advmod	when,performed
R1253	T2013	T2011	advcl	performed,lost
R1254	T2014	T2013	nsubj	we,performed
R1255	T2015	T2016	amod	secondary,structure
R1256	T2016	T2017	compound	structure,prediction
R1257	T2017	T2018	compound	prediction,analysis
R1258	T2018	T2013	dobj	analysis,performed
R1259	T2019	T2013	prep	on,performed
R1260	T2020	T2021	det	this,peptide
R1261	T2021	T2019	pobj	peptide,on
R1262	T2022	T2023	nummod	28,acid
R1263	T2023	T2021	compound	acid,peptide
R1264	T2024	T2023	compound	amino,acid
R1265	T2025	T2011	punct	", ",lost
R1266	T2026	T2027	det	the,sheet
R1267	T2027	T2011	nsubjpass	sheet,lost
R1268	T2028	T2027	amod	first,sheet
R1269	T2029	T2027	compound	beta,sheet
R1270	T2030	T2011	auxpass	was,lost
R1271	T2031	T2032	punct	(,Figure
R1272	T2032	T2011	parataxis	Figure,lost
R1273	T2033	T2032	nummod	1b,Figure
R1274	T2034	T2032	punct	),Figure
R1275	T2035	T2011	punct	.,lost
R1276	T2037	T2038	nsubj	Addition,caused
R1277	T2039	T2037	prep	of,Addition
R1278	T2040	T2041	det	another,acids
R1279	T2041	T2039	pobj	acids,of
R1280	T2042	T2041	nummod	4,acids
R1281	T2043	T2041	compound	amino,acids
R1282	T2044	T2045	punct	(,TTST
R1283	T2045	T2037	parataxis	TTST,Addition
R1284	T2046	T2045	punct	),TTST
R1285	T2047	T2048	advmod	immediately,before
R1286	T2048	T2037	prep	before,Addition
R1287	T2049	T2050	punct	',GLISP
R1288	T2050	T2048	pobj	GLISP,before
R1289	T2051	T2050	punct	',GLISP
R1290	T2052	T2038	dobj	recovery,caused
R1291	T2053	T2052	prep	of,recovery
R1292	T2054	T2055	det	the,sheet
R1293	T2055	T2053	pobj	sheet,of
R1294	T2056	T2055	amod	first,sheet
R1295	T2057	T2055	compound	beta,sheet
R1296	T2058	T2059	punct	(,Figure
R1297	T2059	T2038	parataxis	Figure,caused
R1298	T2060	T2059	nummod	1c,Figure
R1299	T2061	T2059	punct	),Figure
R1300	T2062	T2038	punct	.,caused
R1301	T2064	T2065	mark	Although,are
R1302	T2065	T2069	advcl	are,appear
R1303	T2066	T2067	det	these,residues
R1304	T2067	T2065	nsubj	residues,are
R1305	T2068	T2067	nummod	4,residues
R1306	T2070	T2065	neg	not,are
R1307	T2071	T2072	advmod	highly,conserved
R1308	T2072	T2065	acomp	conserved,are
R1309	T2073	T2074	punct	(,Figure
R1310	T2074	T2065	parataxis	Figure,are
R1311	T2075	T2074	nummod	1a,Figure
R1312	T2076	T2074	punct	),Figure
R1313	T2077	T2069	punct	", ",appear
R1314	T2078	T2069	nsubj	they,appear
R1315	T2079	T2080	aux	to,be
R1316	T2080	T2069	xcomp	be,appear
R1317	T2081	T2080	acomp	important,be
R1318	T2082	T2081	prep	for,important
R1319	T2083	T2082	pcomp	seeding,for
R1320	T2084	T2085	det	the,sheet
R1321	T2085	T2083	dobj	sheet,seeding
R1322	T2086	T2085	amod	first,sheet
R1323	T2087	T2085	compound	beta,sheet
R1324	T2088	T2082	cc	and,for
R1325	T2089	T2090	advmod	therefore,for
R1326	T2090	T2082	conj	for,for
R1327	T2091	T2092	amod	secondary,structure
R1328	T2092	T2090	pobj	structure,for
R1329	T2093	T2069	punct	.,appear
R1330	T2095	T2096	advmod	Thus,define
R1331	T2097	T2096	nsubj	we,define
R1332	T2098	T2099	det	the,peptide
R1333	T2099	T2096	dobj	peptide,define
R1334	T2100	T2101	npadvmod	C,terminal
R1335	T2101	T2099	amod	terminal,peptide
R1336	T2102	T2101	punct	-,terminal
R1337	T2103	T2096	prep	as,define
R1338	T2104	T2103	pcomp	being,as
R1339	T2105	T2106	det	the,acids
R1340	T2106	T2104	attr	acids,being
R1341	T2107	T2106	amod	final,acids
R1342	T2108	T2106	nummod	32,acids
R1343	T2109	T2106	compound	amino,acids
R1344	T2110	T2106	prep	of,acids
R1345	T2111	T2110	pobj	PAX6,of
R1346	T2112	T2106	punct	", ",acids
R1347	T2113	T2106	acl	running,acids
R1348	T2114	T2113	prep	from,running
R1349	T2115	T2114	pobj	threonine,from
R1350	T2116	T2115	nummod	391,threonine
R1351	T2117	T2113	prep	to,running
R1352	T2118	T2119	det	the,codon
R1353	T2119	T2117	pobj	codon,to
R1354	T2120	T2119	compound	stop,codon
R1355	T2121	T2122	punct	(,line
R1356	T2122	T2096	parataxis	line,define
R1357	T2123	T2122	compound	top,line
R1358	T2124	T2122	prep	of,line
R1359	T2125	T2124	pobj	Figure,of
R1360	T2126	T2125	nummod	1c,Figure
R1361	T2127	T2122	punct	),line
R1362	T2128	T2096	punct	.,define
R1365	T2344	T2345	nmod	Yeast,screening
R1366	T2346	T2347	nummod	two,hybrid
R1367	T2347	T2345	compound	hybrid,screening
R1368	T2348	T2347	punct	-,hybrid
R1369	T2350	T2351	nsubj	We,hypothesised
R1370	T2352	T2353	mark	that,involved
R1371	T2353	T2351	ccomp	involved,hypothesised
R1372	T2354	T2355	det	the,peptide
R1373	T2355	T2353	nsubjpass	peptide,involved
R1374	T2356	T2357	npadvmod	C,terminal
R1375	T2357	T2355	amod	terminal,peptide
R1376	T2358	T2357	punct	-,terminal
R1377	T2359	T2353	aux	might,involved
R1378	T2360	T2353	auxpass	be,involved
R1379	T2361	T2353	prep	in,involved
R1380	T2362	T2363	nmod	protein,protein
R1381	T2363	T2365	compound	protein,interactions
R1382	T2364	T2363	punct	-,protein
R1383	T2365	T2361	pobj	interactions,in
R1384	T2366	T2351	punct	", ",hypothesised
R1385	T2367	T2351	cc	and,hypothesised
R1386	T2368	T2369	nsubj	we,tested
R1387	T2369	T2351	conj	tested,hypothesised
R1388	T2370	T2369	dobj	this,tested
R1389	T2371	T2369	prep	by,tested
R1390	T2372	T2371	pcomp	screening,by
R1391	T2373	T2374	det	a,library
R1392	T2374	T2372	dobj	library,screening
R1393	T2375	T2374	compound	cDNA,library
R1394	T2376	T2372	advcl	using,screening
R1395	T2377	T2378	det	the,system
R1396	T2378	T2376	dobj	system,using
R1397	T2379	T2378	nmod	yeast,system
R1398	T2380	T2381	nummod	two,hybrid
R1399	T2381	T2378	compound	hybrid,system
R1400	T2382	T2381	punct	-,hybrid
R1401	T2383	T2378	prep	with,system
R1402	T2384	T2385	det	a,construct
R1403	T2385	T2383	pobj	construct,with
R1404	T2386	T2387	punct	(,PAX6CTP
R1405	T2387	T2385	parataxis	PAX6CTP,construct
R1406	T2388	T2387	punct	),PAX6CTP
R1407	T2389	T2390	prep	in,fused
R1408	T2390	T2385	relcl	fused,construct
R1409	T2391	T2389	pobj	which,in
R1410	T2392	T2393	det	the,peptide
R1411	T2393	T2390	nsubjpass	peptide,fused
R1412	T2394	T2395	nummod	32,acid
R1413	T2395	T2393	nmod	acid,peptide
R1414	T2396	T2395	nmod	amino,acid
R1415	T2397	T2398	npadvmod	C,terminal
R1416	T2398	T2393	amod	terminal,peptide
R1417	T2399	T2398	punct	-,terminal
R1418	T2400	T2390	auxpass	was,fused
R1419	T2401	T2390	prep	to,fused
R1420	T2402	T2403	det	the,domain
R1421	T2403	T2401	pobj	domain,to
R1422	T2404	T2403	compound	yeast,domain
R1423	T2405	T2403	compound	GAL4,domain
R1424	T2406	T2407	compound	DNA,binding
R1425	T2407	T2403	compound	binding,domain
R1426	T2408	T2369	punct	.,tested
R1427	T2410	T2411	nsubj	We,chose
R1428	T2412	T2413	aux	to,screen
R1429	T2413	T2411	xcomp	screen,chose
R1430	T2414	T2415	det	a,library
R1431	T2415	T2413	dobj	library,screen
R1432	T2416	T2417	compound	mouse,brain
R1433	T2417	T2415	compound	brain,library
R1434	T2418	T2415	compound	cDNA,library
R1435	T2419	T2420	mark	as,were
R1436	T2420	T2411	advcl	were,chose
R1437	T2421	T2422	det	no,libraries
R1438	T2422	T2420	nsubj	libraries,were
R1439	T2423	T2422	amod	human,libraries
R1440	T2424	T2420	acomp	available,were
R1441	T2425	T2411	punct	.,chose
R1442	T2427	T2428	prep	Given,reasoned
R1443	T2429	T2430	det	the,fact
R1444	T2430	T2427	pobj	fact,Given
R1445	T2431	T2432	mark	that,is
R1446	T2432	T2430	acl	is,fact
R1447	T2433	T2434	det	the,sequence
R1448	T2434	T2432	nsubj	sequence,is
R1449	T2435	T2436	compound	amino,acid
R1450	T2436	T2434	compound	acid,sequence
R1451	T2437	T2434	prep	of,sequence
R1452	T2438	T2439	det	the,protein
R1453	T2439	T2437	pobj	protein,of
R1454	T2440	T2439	compound	PAX6,protein
R1455	T2441	T2432	acomp	identical,is
R1456	T2442	T2432	prep	in,is
R1457	T2443	T2442	pobj	man,in
R1458	T2444	T2443	cc	and,man
R1459	T2445	T2443	conj	mouse,man
R1460	T2446	T2428	punct	", ",reasoned
R1461	T2447	T2428	nsubj	we,reasoned
R1462	T2448	T2449	mark	that,yield
R1463	T2449	T2428	ccomp	yield,reasoned
R1464	T2450	T2451	det	a,library
R1465	T2451	T2449	nsubj	library,yield
R1466	T2452	T2453	compound	mouse,brain
R1467	T2453	T2451	compound	brain,library
R1468	T2454	T2449	aux	would,yield
R1469	T2455	T2456	amod	relevant,interactors
R1470	T2456	T2449	dobj	interactors,yield
R1471	T2457	T2428	punct	.,reasoned
R1472	T2459	T2460	prep	For,carried
R1473	T2461	T2459	pobj	comparison,For
R1474	T2462	T2460	nsubj	we,carried
R1475	T2463	T2460	advmod	also,carried
R1476	T2464	T2460	prt	out,carried
R1477	T2465	T2466	det	the,screen
R1478	T2466	T2460	dobj	screen,carried
R1479	T2467	T2460	advcl	using,carried
R1480	T2468	T2469	det	a,construct
R1481	T2469	T2467	dobj	construct,using
R1482	T2470	T2469	acl	containing,construct
R1483	T2471	T2472	det	the,domain
R1484	T2472	T2470	dobj	domain,containing
R1485	T2473	T2472	amod	whole,domain
R1486	T2474	T2472	compound	PST,domain
R1487	T2475	T2469	punct	(,construct
R1488	T2476	T2469	appos	PAX6PST,construct
R1489	T2477	T2469	punct	),construct
R1490	T2478	T2460	punct	.,carried
R1491	T2480	T2481	det	The,screen
R1492	T2481	T2486	nsubj	screen,gave
R1493	T2482	T2483	npadvmod	C,terminal
R1494	T2483	T2481	amod	terminal,screen
R1495	T2484	T2483	punct	-,terminal
R1496	T2485	T2481	compound	peptide,screen
R1497	T2487	T2488	nummod	15,colonies
R1498	T2488	T2486	dobj	colonies,gave
R1499	T2489	T2490	dep	that,were
R1500	T2490	T2488	relcl	were,colonies
R1501	T2491	T2490	acomp	positive,were
R1502	T2492	T2491	prep	with,positive
R1503	T2493	T2494	det	all,reporters
R1504	T2494	T2492	pobj	reporters,with
R1505	T2495	T2494	nummod	three,reporters
R1506	T2496	T2486	cc	and,gave
R1507	T2497	T2498	det	the,screen
R1508	T2498	T2501	nsubj	screen,gave
R1509	T2499	T2498	compound	PST,screen
R1510	T2500	T2498	compound	domain,screen
R1511	T2501	T2486	conj	gave,gave
R1512	T2502	T2503	nummod	62,colonies
R1513	T2503	T2501	dobj	colonies,gave
R1514	T2504	T2501	punct	.,gave
R1515	T2506	T2507	det	The,plasmids
R1516	T2507	T2509	nsubjpass	plasmids,isolated
R1517	T2508	T2507	amod	interacting,plasmids
R1518	T2510	T2509	auxpass	were,isolated
R1519	T2511	T2509	cc	and,isolated
R1520	T2512	T2513	det	the,inserts
R1521	T2513	T2515	nsubj	inserts,sequenced
R1522	T2514	T2513	compound	cDNA,inserts
R1523	T2515	T2509	conj	sequenced,isolated
R1524	T2516	T2515	punct	.,sequenced
R1525	T2518	T2519	nummod	Three,cDNAs
R1526	T2519	T2520	nsubjpass	cDNAs,identified
R1527	T2521	T2520	auxpass	were,identified
R1528	T2522	T2523	nummod	3,times
R1529	T2523	T2520	npadvmod	times,identified
R1530	T2524	T2522	cc	or,3
R1531	T2525	T2522	conj	more,3
R1532	T2526	T2520	punct	", ",identified
R1533	T2527	T2520	dep	Homer3,identified
R1534	T2528	T2527	punct	(,Homer3
R1535	T2529	T2527	appos	NM_011984,Homer3
R1536	T2530	T2527	punct	),Homer3
R1537	T2531	T2527	punct	", ",Homer3
R1538	T2532	T2527	conj	Dncl1,Homer3
R1539	T2533	T2532	punct	(,Dncl1
R1540	T2534	T2535	nmod	Dynein,chain
R1541	T2535	T2532	appos	chain,Dncl1
R1542	T2536	T2535	amod	cytoplasmic,chain
R1543	T2537	T2535	amod	light,chain
R1544	T2538	T2535	nummod	1,chain
R1545	T2539	T2535	punct	", ",chain
R1546	T2540	T2535	appos	NM_019682,chain
R1547	T2541	T2535	punct	", ",chain
R1548	T2542	T2543	advmod	also,known
R1549	T2543	T2535	acl	known,chain
R1550	T2544	T2543	prep	as,known
R1551	T2545	T2544	pobj	Pin,as
R1552	T2546	T2545	cc	or,Pin
R1553	T2547	T2545	conj	Dlc8,Pin
R1554	T2548	T2532	punct	),Dncl1
R1555	T2549	T2532	cc	and,Dncl1
R1556	T2550	T2532	conj	Trim11,Dncl1
R1557	T2551	T2550	punct	(,Trim11
R1558	T2552	T2553	amod	Tripartite,motif
R1559	T2553	T2554	compound	motif,member
R1560	T2554	T2550	appos	member,Trim11
R1561	T2555	T2554	compound	protein,member
R1562	T2556	T2554	compound	family,member
R1563	T2557	T2554	nummod	11,member
R1564	T2558	T2554	punct	", ",member
R1565	T2559	T2554	appos	NM_053168,member
R1566	T2560	T2550	punct	),Trim11
R1567	T2561	T2520	punct	.,identified
R1568	T2563	T2564	nsubjpass	Homer3,identified
R1569	T2565	T2566	punct	(,clones
R1570	T2566	T2563	parataxis	clones,Homer3
R1571	T2567	T2566	nummod	6,clones
R1572	T2568	T2566	punct	),clones
R1573	T2569	T2563	cc	and,Homer3
R1574	T2570	T2563	conj	Dncl1,Homer3
R1575	T2571	T2572	punct	(,clones
R1576	T2572	T2570	parataxis	clones,Dncl1
R1577	T2573	T2572	nummod	2,clones
R1578	T2574	T2572	punct	),clones
R1579	T2575	T2564	auxpass	were,identified
R1580	T2576	T2564	prep	in,identified
R1581	T2577	T2578	det	the,screen
R1582	T2578	T2576	pobj	screen,in
R1583	T2579	T2580	npadvmod	C,terminal
R1584	T2580	T2578	amod	terminal,screen
R1585	T2581	T2580	punct	-,terminal
R1586	T2582	T2578	compound	peptide,screen
R1587	T2583	T2564	punct	.,identified
R1588	T2585	T2586	nsubjpass	Homer3,identified
R1589	T2587	T2588	punct	(,clones
R1590	T2588	T2585	parataxis	clones,Homer3
R1591	T2589	T2588	nummod	7,clones
R1592	T2590	T2588	punct	),clones
R1593	T2591	T2585	punct	", ",Homer3
R1594	T2592	T2585	conj	Dncl1,Homer3
R1595	T2593	T2594	punct	(,clones
R1596	T2594	T2592	parataxis	clones,Dncl1
R1597	T2595	T2594	nummod	2,clones
R1598	T2596	T2594	punct	),clones
R1599	T2597	T2592	cc	and,Dncl1
R1600	T2598	T2592	conj	Trim11,Dncl1
R1601	T2599	T2600	punct	(,clones
R1602	T2600	T2598	parataxis	clones,Trim11
R1603	T2601	T2600	nummod	6,clones
R1604	T2602	T2600	punct	),clones
R1605	T2603	T2586	auxpass	were,identified
R1606	T2604	T2586	prep	in,identified
R1607	T2605	T2606	det	the,screen
R1608	T2606	T2604	pobj	screen,in
R1609	T2607	T2606	compound	PST,screen
R1610	T2608	T2606	compound	domain,screen
R1611	T2609	T2586	punct	.,identified
R1612	T2611	T2612	det	All,inserts
R1613	T2612	T2614	nsubj	inserts,were
R1614	T2613	T2612	compound	cDNA,inserts
R1615	T2615	T2614	prep	in,were
R1616	T2616	T2615	punct	-,in
R1617	T2617	T2615	pobj	frame,in
R1618	T2618	T2617	prep	with,frame
R1619	T2619	T2620	det	the,region
R1620	T2620	T2618	pobj	region,with
R1621	T2621	T2620	compound	coding,region
R1622	T2622	T2620	prep	of,region
R1623	T2623	T2624	det	the,domain
R1624	T2624	T2622	pobj	domain,of
R1625	T2625	T2624	compound	pPC86,domain
R1626	T2626	T2624	compound	GAL4,domain
R1627	T2627	T2624	compound	activation,domain
R1628	T2628	T2614	punct	.,were
R1629	T2630	T2631	nsubj	None,was
R1630	T2632	T2630	prep	of,None
R1631	T2633	T2634	det	the,cDNAs
R1632	T2634	T2632	pobj	cDNAs,of
R1633	T2635	T2631	acomp	present,was
R1634	T2636	T2631	prep	in,was
R1635	T2637	T2638	det	a,list
R1636	T2638	T2636	pobj	list,in
R1637	T2639	T2638	prep	of,list
R1638	T2640	T2641	amod	known,positives
R1639	T2641	T2639	pobj	positives,of
R1640	T2642	T2641	amod	false,positives
R1641	T2643	T2644	punct	[,34
R1642	T2644	T2631	parataxis	34,was
R1643	T2645	T2644	punct	],34
R1644	T2646	T2631	punct	.,was
R1645	T2648	T2649	nsubj	HOMER3,is
R1646	T2650	T2651	det	a,member
R1647	T2651	T2649	attr	member,is
R1648	T2652	T2651	prep	of,member
R1649	T2653	T2654	det	the,family
R1650	T2654	T2652	pobj	family,of
R1651	T2655	T2654	compound	HOMER,family
R1652	T2656	T2654	prep	of,family
R1653	T2657	T2658	amod	neuronal,proteins
R1654	T2658	T2656	pobj	proteins,of
R1655	T2659	T2660	amod	post-synaptic,density
R1656	T2660	T2658	nmod	density,proteins
R1657	T2661	T2660	punct	(,density
R1658	T2662	T2660	appos	PSD,density
R1659	T2663	T2658	punct	),proteins
R1660	T2664	T2665	punct	[,35
R1661	T2665	T2649	parataxis	35,is
R1662	T2666	T2665	punct	],35
R1663	T2667	T2649	punct	.,is
R1664	T2669	T2670	nsubj	DNCL1,is
R1665	T2671	T2672	det	a,subunit
R1666	T2672	T2670	attr	subunit,is
R1667	T2673	T2672	prep	of,subunit
R1668	T2674	T2675	nummod	two,complexes
R1669	T2675	T2673	pobj	complexes,of
R1670	T2676	T2675	compound	motor,complexes
R1671	T2677	T2675	compound	protein,complexes
R1672	T2678	T2675	punct	", ",complexes
R1673	T2679	T2675	appos	dynein,complexes
R1674	T2680	T2679	cc	and,dynein
R1675	T2681	T2682	compound	myosin,Va
R1676	T2682	T2679	conj	Va,dynein
R1677	T2683	T2682	punct	-,Va
R1678	T2684	T2679	punct	", ",dynein
R1679	T2685	T2686	dep	both,involved
R1680	T2686	T2679	relcl	involved,dynein
R1681	T2687	T2685	prep	of,both
R1682	T2688	T2687	pobj	which,of
R1683	T2689	T2686	auxpass	are,involved
R1684	T2690	T2686	prep	in,involved
R1685	T2691	T2692	amod	intracellular,trafficking
R1686	T2692	T2690	pobj	trafficking,in
R1687	T2693	T2692	prep	of,trafficking
R1688	T2694	T2693	pobj	proteins,of
R1689	T2695	T2694	cc	and,proteins
R1690	T2696	T2694	conj	organelles,proteins
R1691	T2697	T2686	prep	in,involved
R1692	T2698	T2697	pobj	neurons,in
R1693	T2699	T2700	punct	[,37
R1694	T2700	T2670	parataxis	37,is
R1695	T2701	T2700	nummod	36,37
R1696	T2702	T2700	punct	",",37
R1697	T2703	T2700	punct	],37
R1698	T2704	T2670	punct	.,is
R1699	T2706	T2707	nsubj	TRIM11,is
R1700	T2708	T2709	det	a,member
R1701	T2709	T2707	attr	member,is
R1702	T2710	T2709	prep	of,member
R1703	T2711	T2712	det	the,family
R1704	T2712	T2710	pobj	family,of
R1705	T2713	T2714	amod	tripartite,motif
R1706	T2714	T2712	compound	motif,family
R1707	T2715	T2712	compound	protein,family
R1708	T2716	T2707	cc	and,is
R1709	T2717	T2707	conj	contains,is
R1710	T2718	T2719	det	a,finger
R1711	T2719	T2717	dobj	finger,contains
R1712	T2720	T2719	compound	RING,finger
R1713	T2721	T2719	punct	", ",finger
R1714	T2722	T2723	det	a,finger
R1715	T2723	T2719	conj	finger,finger
R1716	T2724	T2725	compound	B,box
R1717	T2725	T2723	compound	box,finger
R1718	T2726	T2725	punct	-,box
R1719	T2727	T2723	compound	zinc,finger
R1720	T2728	T2723	punct	", ",finger
R1721	T2729	T2730	det	a,domain
R1722	T2730	T2723	conj	domain,finger
R1723	T2731	T2732	amod	coiled,coil
R1724	T2732	T2730	compound	coil,domain
R1725	T2733	T2730	cc	and,domain
R1726	T2734	T2735	det	a,domain
R1727	T2735	T2730	conj	domain,domain
R1728	T2736	T2735	compound	B30.2,domain
R1729	T2737	T2738	punct	[,38
R1730	T2738	T2717	parataxis	38,contains
R1731	T2739	T2738	punct	],38
R1732	T2740	T2707	punct	.,is
R1733	T2742	T2743	det	The,significance
R1734	T2743	T2745	nsubjpass	significance,discussed
R1735	T2744	T2743	amod	possible,significance
R1736	T2746	T2743	prep	of,significance
R1737	T2747	T2748	det	the,interactions
R1738	T2748	T2746	pobj	interactions,of
R1739	T2749	T2748	prep	between,interactions
R1740	T2750	T2751	det	these,proteins
R1741	T2751	T2749	pobj	proteins,between
R1742	T2752	T2751	cc	and,proteins
R1743	T2753	T2751	conj	PAX6,proteins
R1744	T2754	T2745	auxpass	is,discussed
R1745	T2755	T2745	advmod	below,discussed
R1746	T2756	T2745	punct	.,discussed
R1747	T2839	T2840	amod	Semi-quantitative,PCR
R1748	T2842	T2843	aux	To,check
R1749	T2843	T2844	advcl	check,performed
R1750	T2845	T2846	mark	that,identified
R1751	T2846	T2843	ccomp	identified,check
R1752	T2847	T2848	det	the,clones
R1753	T2848	T2846	nsubjpass	clones,identified
R1754	T2849	T2848	nmod	Homer3,clones
R1755	T2850	T2849	punct	", ",Homer3
R1756	T2851	T2849	conj	Dncl1,Homer3
R1757	T2852	T2851	cc	and,Dncl1
R1758	T2853	T2851	conj	Trim11,Dncl1
R1759	T2854	T2846	auxpass	were,identified
R1760	T2855	T2846	neg	not,identified
R1761	T2856	T2857	amod	multiple,times
R1762	T2857	T2846	npadvmod	times,identified
R1763	T2858	T2859	advmod	solely,are
R1764	T2859	T2846	advcl	are,identified
R1765	T2860	T2859	mark	because,are
R1766	T2861	T2859	nsubj	they,are
R1767	T2862	T2863	advmod	highly,abundant
R1768	T2863	T2859	acomp	abundant,are
R1769	T2864	T2859	prep	in,are
R1770	T2865	T2866	det	the,library
R1771	T2866	T2864	pobj	library,in
R1772	T2867	T2844	punct	", ",performed
R1773	T2868	T2844	nsubj	we,performed
R1774	T2869	T2870	det	a,assay
R1775	T2870	T2844	dobj	assay,performed
R1776	T2871	T2870	amod	semi-quantitative,assay
R1777	T2872	T2870	compound	PCR,assay
R1778	T2873	T2844	punct	.,performed
R1779	T2875	T2876	nsubj	We,compared
R1780	T2877	T2878	det	the,abundance
R1781	T2878	T2876	dobj	abundance,compared
R1782	T2879	T2878	amod	relative,abundance
R1783	T2880	T2878	prep	of,abundance
R1784	T2881	T2880	pobj	Homer3,of
R1785	T2882	T2881	punct	", ",Homer3
R1786	T2883	T2881	conj	Dncl1,Homer3
R1787	T2884	T2883	punct	", ",Dncl1
R1788	T2885	T2883	conj	Trim11,Dncl1
R1789	T2886	T2885	cc	and,Trim11
R1790	T2887	T2885	conj	Pax6,Trim11
R1791	T2888	T2876	prep	with,compared
R1792	T2889	T2888	pobj	Gapdh,with
R1793	T2890	T2889	cc	and,Gapdh
R1794	T2891	T2889	conj	Atp5a1,Gapdh
R1795	T2892	T2876	punct	.,compared
R1796	T2894	T2895	nsubj	Gapdh,show
R1797	T2896	T2894	cc	and,Gapdh
R1798	T2897	T2894	conj	Atp5a1,Gapdh
R1799	T2898	T2894	appos	both,Gapdh
R1800	T2899	T2900	amod	strong,expression
R1801	T2900	T2895	dobj	expression,show
R1802	T2901	T2900	amod	constitutive,expression
R1803	T2902	T2895	prep	in,show
R1804	T2903	T2904	det	a,variety
R1805	T2904	T2902	pobj	variety,in
R1806	T2905	T2904	prep	of,variety
R1807	T2906	T2905	pobj	tissues,of
R1808	T2907	T2906	prep	including,tissues
R1809	T2908	T2909	det	the,brain
R1810	T2909	T2907	pobj	brain,including
R1811	T2910	T2911	punct	[,30
R1812	T2911	T2895	parataxis	30,show
R1813	T2912	T2911	nummod	29,30
R1814	T2913	T2911	punct	",",30
R1815	T2914	T2911	punct	],30
R1816	T2915	T2895	punct	.,show
R1817	T2917	T2918	nsubjpass	Homer3,amplified
R1818	T2919	T2917	punct	", ",Homer3
R1819	T2920	T2917	conj	Dncl1,Homer3
R1820	T2921	T2920	punct	", ",Dncl1
R1821	T2922	T2920	conj	Trim11,Dncl1
R1822	T2923	T2922	cc	and,Trim11
R1823	T2924	T2922	conj	Pax6,Trim11
R1824	T2925	T2918	auxpass	were,amplified
R1825	T2926	T2918	advmod	only,amplified
R1826	T2927	T2918	advmod	strongly,amplified
R1827	T2928	T2918	prep	after,amplified
R1828	T2929	T2930	nummod	35,cycles
R1829	T2930	T2928	pobj	cycles,after
R1830	T2931	T2930	prep	of,cycles
R1831	T2932	T2931	pobj	PCR,of
R1832	T2933	T2934	punct	(,Figure
R1833	T2934	T2918	parataxis	Figure,amplified
R1834	T2935	T2934	nummod	2,Figure
R1835	T2936	T2934	punct	),Figure
R1836	T2937	T2918	cc	and,amplified
R1837	T2938	T2918	conj	were,amplified
R1838	T2939	T2938	advmod	therefore,were
R1839	T2940	T2938	acomp	present,were
R1840	T2941	T2940	prep	at,present
R1841	T2942	T2943	advmod	relatively,low
R1842	T2943	T2944	amod	low,levels
R1843	T2944	T2941	pobj	levels,at
R1844	T2945	T2938	prep	compared,were
R1845	T2946	T2945	prep	to,compared
R1846	T2947	T2946	pobj	Gapdh,to
R1847	T2948	T2947	punct	(,Gapdh
R1848	T2949	T2947	acl	amplified,Gapdh
R1849	T2950	T2949	advmod	strongly,amplified
R1850	T2951	T2949	prep	after,amplified
R1851	T2952	T2953	nummod	25,cycles
R1852	T2953	T2951	pobj	cycles,after
R1853	T2954	T2947	punct	),Gapdh
R1854	T2955	T2947	cc	and,Gapdh
R1855	T2956	T2947	conj	Atp5a1,Gapdh
R1856	T2957	T2956	punct	(,Atp5a1
R1857	T2958	T2956	acl	amplified,Atp5a1
R1858	T2959	T2958	advmod	strongly,amplified
R1859	T2960	T2958	prep	after,amplified
R1860	T2961	T2962	nummod	30,cycles
R1861	T2962	T2960	pobj	cycles,after
R1862	T2963	T2964	punct	;,Figure
R1863	T2964	T2938	parataxis	Figure,were
R1864	T2965	T2964	nummod	2,Figure
R1865	T2966	T2964	punct	),Figure
R1866	T2967	T2918	punct	.,amplified
R1867	T2969	T2970	nsubj	We,concluded
R1868	T2971	T2972	mark	that,were
R1869	T2972	T2970	ccomp	were,concluded
R1870	T2973	T2974	nmod	Homer3,clones
R1871	T2974	T2972	nsubj	clones,were
R1872	T2975	T2973	punct	", ",Homer3
R1873	T2976	T2973	conj	Dncl1,Homer3
R1874	T2977	T2976	cc	and,Dncl1
R1875	T2978	T2976	conj	Trim11,Dncl1
R1876	T2979	T2972	neg	not,were
R1877	T2980	T2981	advmod	highly,abundant
R1878	T2981	T2972	acomp	abundant,were
R1879	T2982	T2972	prep	in,were
R1880	T2983	T2984	det	the,library
R1881	T2984	T2982	pobj	library,in
R1882	T2985	T2970	punct	.,concluded
R1883	T2987	T2988	nsubj	This,is
R1884	T2989	T2988	acomp	consistent,is
R1885	T2990	T2989	prep	with,consistent
R1886	T2991	T2992	det	the,idea
R1887	T2992	T2990	pobj	idea,with
R1888	T2993	T2994	mark	that,pulled
R1889	T2994	T2992	acl	pulled,idea
R1890	T2995	T2994	nsubjpass	they,pulled
R1891	T2996	T2994	auxpass	were,pulled
R1892	T2997	T2994	prt	out,pulled
R1893	T2998	T2999	mark	because,interact
R1894	T2999	T2994	advcl	interact,pulled
R1895	T3000	T3001	det	the,proteins
R1896	T3001	T2999	nsubj	proteins,interact
R1897	T3002	T3001	amod	encoded,proteins
R1898	T3003	T2999	advmod	specifically,interact
R1899	T3004	T2999	prep	with,interact
R1900	T3005	T3006	det	the,peptide
R1901	T3006	T3004	pobj	peptide,with
R1902	T3007	T3008	npadvmod	C,terminal
R1903	T3008	T3006	amod	terminal,peptide
R1904	T3009	T3008	punct	-,terminal
R1905	T3010	T3006	cc	or,peptide
R1906	T3011	T3012	compound	PST,domain
R1907	T3012	T3006	conj	domain,peptide
R1908	T3013	T3012	prep	of,domain
R1909	T3014	T3013	pobj	PAX6,of
R1910	T3015	T2988	punct	.,is
R1911	T3382	T3383	nmod	Yeast,interactions
R1912	T3384	T3385	nummod	two,hybrid
R1913	T3385	T3383	nmod	hybrid,interactions
R1914	T3386	T3385	punct	-,hybrid
R1915	T3387	T3383	amod	pairwise,interactions
R1916	T3389	T3390	prep	By,identified
R1917	T3391	T3392	compound	library,screening
R1918	T3392	T3389	pobj	screening,By
R1919	T3393	T3390	nsubj	we,identified
R1920	T3394	T3395	nummod	two,proteins
R1921	T3395	T3390	dobj	proteins,identified
R1922	T3396	T3395	punct	(,proteins
R1923	T3397	T3395	appos	HOMER3,proteins
R1924	T3398	T3397	cc	and,HOMER3
R1925	T3399	T3397	conj	DNCL1,HOMER3
R1926	T3400	T3395	punct	),proteins
R1927	T3401	T3402	dep	that,interact
R1928	T3402	T3395	relcl	interact,proteins
R1929	T3403	T3402	prep	with,interact
R1930	T3404	T3405	det	the,peptide
R1931	T3405	T3403	pobj	peptide,with
R1932	T3406	T3407	npadvmod	C,terminal
R1933	T3407	T3405	amod	terminal,peptide
R1934	T3408	T3407	punct	-,terminal
R1935	T3409	T3405	cc	and,peptide
R1936	T3410	T3411	nummod	three,proteins
R1937	T3411	T3405	conj	proteins,peptide
R1938	T3412	T3411	punct	(,proteins
R1939	T3413	T3411	appos	HOMER3,proteins
R1940	T3414	T3413	punct	", ",HOMER3
R1941	T3415	T3413	conj	DNCL1,HOMER3
R1942	T3416	T3415	cc	and,DNCL1
R1943	T3417	T3415	conj	TRIM11,DNCL1
R1944	T3418	T3411	punct	),proteins
R1945	T3419	T3420	dep	that,interact
R1946	T3420	T3411	relcl	interact,proteins
R1947	T3421	T3420	prep	with,interact
R1948	T3422	T3423	det	the,domain
R1949	T3423	T3421	pobj	domain,with
R1950	T3424	T3423	amod	whole,domain
R1951	T3425	T3423	compound	PST,domain
R1952	T3426	T3390	punct	.,identified
R1953	T3428	T3429	nsubj	This,suggests
R1954	T3430	T3431	mark	that,interact
R1955	T3431	T3429	ccomp	interact,suggests
R1956	T3432	T3431	nsubj	HOMER3,interact
R1957	T3433	T3432	cc	and,HOMER3
R1958	T3434	T3432	conj	DNCL1,HOMER3
R1959	T3435	T3436	advmod	specifically,with
R1960	T3436	T3431	prep	with,interact
R1961	T3437	T3438	det	the,peptide
R1962	T3438	T3436	pobj	peptide,with
R1963	T3439	T3440	npadvmod	C,terminal
R1964	T3440	T3438	amod	terminal,peptide
R1965	T3441	T3440	punct	-,terminal
R1966	T3442	T3443	mark	while,interacts
R1967	T3443	T3431	advcl	interacts,interact
R1968	T3444	T3443	nsubj	TRIM11,interacts
R1969	T3445	T3443	prep	with,interacts
R1970	T3446	T3447	det	a,part
R1971	T3447	T3445	pobj	part,with
R1972	T3448	T3449	advmod	more,terminal
R1973	T3449	T3447	amod	terminal,part
R1974	T3450	T3449	npadvmod	N,terminal
R1975	T3451	T3449	punct	-,terminal
R1976	T3452	T3447	prep	of,part
R1977	T3453	T3454	det	the,domain
R1978	T3454	T3452	pobj	domain,of
R1979	T3455	T3454	compound	PST,domain
R1980	T3456	T3429	punct	.,suggests
R1981	T3458	T3459	nsubj	We,conducted
R1982	T3460	T3461	amod	pairwise,tests
R1983	T3461	T3459	dobj	tests,conducted
R1984	T3462	T3461	prep	between,tests
R1985	T3463	T3464	amod	specific,constructs
R1986	T3464	T3462	pobj	constructs,between
R1987	T3465	T3466	aux	to,confirm
R1988	T3466	T3459	advcl	confirm,conducted
R1989	T3467	T3468	det	the,interactions
R1990	T3468	T3466	dobj	interactions,confirm
R1991	T3469	T3468	acl	identified,interactions
R1992	T3470	T3469	prep	in,identified
R1993	T3471	T3472	det	the,screen
R1994	T3472	T3470	pobj	screen,in
R1995	T3473	T3472	compound	library,screen
R1996	T3474	T3466	cc	and,confirm
R1997	T3475	T3476	aux	to,investigate
R1998	T3476	T3466	conj	investigate,confirm
R1999	T3477	T3476	advmod	further,investigate
R2000	T3478	T3479	det	the,interaction
R2001	T3479	T3476	dobj	interaction,investigate
R2002	T3480	T3479	prep	between,interaction
R2003	T3481	T3480	pobj	PAX6,between
R2004	T3482	T3481	cc	and,PAX6
R2005	T3483	T3481	conj	HOMER3,PAX6
R2006	T3484	T3481	punct	", ",PAX6
R2007	T3485	T3481	appos	DNCL1,PAX6
R2008	T3486	T3485	cc	and,DNCL1
R2009	T3487	T3485	conj	TRIM11,DNCL1
R2010	T3488	T3459	punct	.,conducted
R2011	T3490	T3491	det	The,clones
R2012	T3491	T3495	nsubj	clones,were
R2013	T3492	T3491	nmod	Dncl1,clones
R2014	T3493	T3492	cc	and,Dncl1
R2015	T3494	T3492	conj	Trim11,Dncl1
R2016	T3496	T3497	dep	that,pulled
R2017	T3497	T3491	relcl	pulled,clones
R2018	T3498	T3497	auxpass	were,pulled
R2019	T3499	T3497	prep	out,pulled
R2020	T3500	T3499	prep	of,out
R2021	T3501	T3502	det	the,library
R2022	T3502	T3500	pobj	library,of
R2023	T3503	T3504	amod	full,length
R2024	T3504	T3495	attr	length,were
R2025	T3505	T3504	punct	-,length
R2026	T3506	T3495	punct	", ",were
R2027	T3507	T3495	cc	but,were
R2028	T3508	T3509	det	the,cDNAs
R2029	T3509	T3511	nsubj	cDNAs,lacked
R2030	T3510	T3509	compound	Homer3,cDNAs
R2031	T3511	T3495	conj	lacked,were
R2032	T3512	T3513	det	the,acids
R2033	T3513	T3511	dobj	acids,lacked
R2034	T3514	T3515	npadvmod	N,terminal
R2035	T3515	T3513	amod	terminal,acids
R2036	T3516	T3515	punct	-,terminal
R2037	T3517	T3513	nummod	70,acids
R2038	T3518	T3513	compound	amino,acids
R2039	T3519	T3511	punct	.,lacked
R2040	T3521	T3522	det	The,region
R2041	T3522	T3525	nsubjpass	region,inserted
R2042	T3523	T3522	amod	missing,region
R2043	T3524	T3522	compound	coding,region
R2044	T3526	T3525	auxpass	was,inserted
R2045	T3527	T3525	prep	into,inserted
R2046	T3528	T3529	det	the,cDNA
R2047	T3529	T3527	pobj	cDNA,into
R2048	T3530	T3529	amod	truncated,cDNA
R2049	T3531	T3529	compound	Homer3,cDNA
R2050	T3532	T3533	aux	to,give
R2051	T3533	T3525	advcl	give,inserted
R2052	T3534	T3535	det	a,construct
R2053	T3535	T3533	dobj	construct,give
R2054	T3536	T3537	amod	full,length
R2055	T3537	T3535	compound	length,construct
R2056	T3538	T3537	punct	-,length
R2057	T3539	T3535	compound	expression,construct
R2058	T3540	T3541	punct	(,see
R2059	T3541	T3525	parataxis	see,inserted
R2060	T3542	T3541	dobj	Methods,see
R2061	T3543	T3541	punct	),see
R2062	T3544	T3525	punct	.,inserted
R2063	T3546	T3547	amod	Pairwise,interactions
R2064	T3547	T3548	nsubjpass	interactions,carried
R2065	T3549	T3548	auxpass	were,carried
R2066	T3550	T3548	prt	out,carried
R2067	T3551	T3548	prep	with,carried
R2068	T3552	T3553	preconj	both,clones
R2069	T3553	T3551	pobj	clones,with
R2070	T3554	T3553	det	the,clones
R2071	T3555	T3556	amod	full,length
R2072	T3556	T3553	nmod	length,clones
R2073	T3557	T3556	punct	-,length
R2074	T3558	T3556	cc	and,length
R2075	T3559	T3556	conj	truncated,length
R2076	T3560	T3553	compound	Homer3,clones
R2077	T3561	T3548	punct	.,carried
R2078	T3563	T3564	nsubj	We,confirmed
R2079	T3565	T3566	mark	that,interacts
R2080	T3566	T3564	ccomp	interacts,confirmed
R2081	T3567	T3568	det	the,domain
R2082	T3568	T3566	nsubj	domain,interacts
R2083	T3569	T3568	amod	whole,domain
R2084	T3570	T3571	compound	PAX6,PST
R2085	T3571	T3568	compound	PST,domain
R2086	T3572	T3566	prep	with,interacts
R2087	T3573	T3572	pobj	HOMER3,with
R2088	T3574	T3575	punct	(,constructs
R2089	T3575	T3573	parataxis	constructs,HOMER3
R2090	T3576	T3577	amod	full,length
R2091	T3577	T3575	nmod	length,constructs
R2092	T3578	T3577	punct	-,length
R2093	T3579	T3577	cc	and,length
R2094	T3580	T3577	conj	truncated,length
R2095	T3581	T3575	punct	),constructs
R2096	T3582	T3573	punct	", ",HOMER3
R2097	T3583	T3573	conj	TRIM11,HOMER3
R2098	T3584	T3583	cc	and,TRIM11
R2099	T3585	T3583	conj	DNCL1,TRIM11
R2100	T3586	T3566	punct	", ",interacts
R2101	T3587	T3588	mark	as,activated
R2102	T3588	T3566	advcl	activated,interacts
R2103	T3589	T3590	det	all,genes
R2104	T3590	T3588	nsubjpass	genes,activated
R2105	T3591	T3590	nummod	three,genes
R2106	T3592	T3590	compound	reporter,genes
R2107	T3593	T3588	auxpass	were,activated
R2108	T3594	T3588	advmod	strongly,activated
R2109	T3595	T3588	prep	in,activated
R2110	T3596	T3597	amod	pairwise,tests
R2111	T3597	T3595	pobj	tests,in
R2112	T3598	T3599	punct	(,Table
R2113	T3599	T3564	parataxis	Table,confirmed
R2114	T3600	T3599	dep	Figure,Table
R2115	T3601	T3600	nummod	3,Figure
R2116	T3602	T3599	punct	;,Table
R2117	T3603	T3599	nummod	1,Table
R2118	T3604	T3599	punct	),Table
R2119	T3605	T3564	punct	.,confirmed
R2120	T3607	T3608	prep	In,confirmed
R2121	T3609	T3607	pobj	contrast,In
R2122	T3610	T3611	det	the,interaction
R2123	T3611	T3608	nsubjpass	interaction,confirmed
R2124	T3612	T3611	prep	between,interaction
R2125	T3613	T3614	det	the,peptide
R2126	T3614	T3612	pobj	peptide,between
R2127	T3615	T3616	npadvmod	C,terminal
R2128	T3616	T3614	amod	terminal,peptide
R2129	T3617	T3616	punct	-,terminal
R2130	T3618	T3614	cc	and,peptide
R2131	T3619	T3614	conj	HOMER3,peptide
R2132	T3620	T3619	cc	or,HOMER3
R2133	T3621	T3619	conj	DNCL1,HOMER3
R2134	T3622	T3608	aux	could,confirmed
R2135	T3623	T3608	neg	not,confirmed
R2136	T3624	T3608	auxpass	be,confirmed
R2137	T3625	T3608	prep	with,confirmed
R2138	T3626	T3627	amod	pairwise,tests
R2139	T3627	T3625	pobj	tests,with
R2140	T3628	T3629	punct	(,Table
R2141	T3629	T3608	parataxis	Table,confirmed
R2142	T3630	T3629	nummod	1,Table
R2143	T3631	T3629	punct	),Table
R2144	T3632	T3608	punct	.,confirmed
R2145	T3634	T3635	advmod	Occasionally,observed
R2146	T3635	T3651	ccomp	observed,observed
R2147	T3636	T3635	punct	", ",observed
R2148	T3637	T3638	amod	partial,suppression
R2149	T3638	T3635	nsubjpass	suppression,observed
R2150	T3639	T3638	prep	of,suppression
R2151	T3640	T3639	pobj	growth,of
R2152	T3641	T3638	prep	on,suppression
R2153	T3642	T3641	pobj	plates,on
R2154	T3643	T3642	acl	containing,plates
R2155	T3644	T3645	advmod	5,orotic
R2156	T3645	T3649	amod	orotic,acid
R2157	T3646	T3645	punct	-,orotic
R2158	T3647	T3645	amod	fluoro,orotic
R2159	T3648	T3645	punct	-,orotic
R2160	T3649	T3643	dobj	acid,containing
R2161	T3650	T3635	auxpass	was,observed
R2162	T3652	T3635	punct	", ",observed
R2163	T3653	T3635	advcl	indicating,observed
R2164	T3654	T3655	amod	low,level
R2165	T3655	T3657	compound	level,activation
R2166	T3656	T3655	punct	-,level
R2167	T3657	T3653	dobj	activation,indicating
R2168	T3658	T3657	prep	of,activation
R2169	T3659	T3660	det	the,reporter
R2170	T3660	T3658	pobj	reporter,of
R2171	T3661	T3660	compound	URA3,reporter
R2172	T3662	T3651	punct	;,observed
R2173	T3663	T3651	advmod	however,observed
R2174	T3664	T3665	nmod	HIS3,activation
R2175	T3665	T3651	nsubjpass	activation,observed
R2176	T3666	T3664	cc	and,HIS3
R2177	T3667	T3664	conj	LacZ,HIS3
R2178	T3668	T3651	auxpass	were,observed
R2179	T3669	T3651	neg	not,observed
R2180	T3670	T3651	punct	.,observed
R2181	T3672	T3673	det	The,reasons
R2182	T3673	T3674	nsubj	reasons,are
R2183	T3675	T3673	prep	for,reasons
R2184	T3676	T3675	pobj	this,for
R2185	T3677	T3674	neg	not,are
R2186	T3678	T3674	acomp	clear,are
R2187	T3679	T3674	punct	", ",are
R2188	T3680	T3681	mark	although,be
R2189	T3681	T3674	advcl	be,are
R2190	T3682	T3681	nsubj	it,be
R2191	T3683	T3681	aux	may,be
R2192	T3684	T3685	mark	that,were
R2193	T3685	T3681	ccomp	were,be
R2194	T3686	T3687	det	the,tests
R2195	T3687	T3685	nsubj	tests,were
R2196	T3688	T3687	amod	pairwise,tests
R2197	T3689	T3685	prep	of,were
R2198	T3690	T3691	amod	sub-optimal,sensitivity
R2199	T3691	T3689	pobj	sensitivity,of
R2200	T3692	T3685	prep	compared,were
R2201	T3693	T3692	prep	to,compared
R2202	T3694	T3695	det	the,screen
R2203	T3695	T3693	pobj	screen,to
R2204	T3696	T3695	compound	library,screen
R2205	T3697	T3674	punct	.,are
R2206	T3699	T3700	advmod	However,were
R2207	T3701	T3700	nsubj	we,were
R2208	T3702	T3700	acomp	able,were
R2209	T3703	T3704	aux	to,confirm
R2210	T3704	T3702	xcomp	confirm,able
R2211	T3705	T3706	mark	that,is
R2212	T3706	T3704	ccomp	is,confirm
R2213	T3707	T3708	det	the,peptide
R2214	T3708	T3706	nsubj	peptide,is
R2215	T3709	T3710	npadvmod	C,terminal
R2216	T3710	T3708	amod	terminal,peptide
R2217	T3711	T3710	punct	-,terminal
R2218	T3712	T3706	acomp	important,is
R2219	T3713	T3712	prep	for,important
R2220	T3714	T3715	det	the,interaction
R2221	T3715	T3713	pobj	interaction,for
R2222	T3716	T3715	prep	with,interaction
R2223	T3717	T3716	pobj	HOMER3,with
R2224	T3718	T3717	cc	and,HOMER3
R2225	T3719	T3717	conj	DNCL1,HOMER3
R2226	T3720	T3721	mark	because,abolished
R2227	T3721	T3706	advcl	abolished,is
R2228	T3722	T3721	nsubjpass	interaction,abolished
R2229	T3723	T3722	prep	with,interaction
R2230	T3724	T3725	det	the,construct
R2231	T3725	T3723	pobj	construct,with
R2232	T3726	T3727	compound	PAX6PST,CT
R2233	T3727	T3725	compound	CT,construct
R2234	T3728	T3727	punct	-,CT
R2235	T3729	T3725	punct	", ",construct
R2236	T3730	T3731	dep	which,lacks
R2237	T3731	T3725	relcl	lacks,construct
R2238	T3732	T3733	det	the,acids
R2239	T3733	T3731	dobj	acids,lacks
R2240	T3734	T3733	amod	final,acids
R2241	T3735	T3733	nummod	32,acids
R2242	T3736	T3733	compound	amino,acids
R2243	T3737	T3721	punct	", ",abolished
R2244	T3738	T3721	auxpass	was,abolished
R2245	T3739	T3721	advmod	completely,abolished
R2246	T3740	T3741	punct	(,Table
R2247	T3741	T3700	parataxis	Table,were
R2248	T3742	T3741	dep	Figure,Table
R2249	T3743	T3742	nummod	3,Figure
R2250	T3744	T3741	punct	", ",Table
R2251	T3745	T3741	nummod	1,Table
R2252	T3746	T3741	punct	),Table
R2253	T3747	T3700	punct	.,were
R2254	T3749	T3750	nsubj	TRIM11,interacted
R2255	T3751	T3752	advmod	equally,well
R2256	T3752	T3750	advmod	well,interacted
R2257	T3753	T3750	prep	with,interacted
R2258	T3754	T3753	pobj	PAX6PST,with
R2259	T3755	T3754	cc	and,PAX6PST
R2260	T3756	T3757	compound	PAX6PST,CT
R2261	T3757	T3754	conj	CT,PAX6PST
R2262	T3758	T3757	punct	-,CT
R2263	T3759	T3760	punct	(,Table
R2264	T3760	T3750	parataxis	Table,interacted
R2265	T3761	T3760	dep	Figure,Table
R2266	T3762	T3761	nummod	3,Figure
R2267	T3763	T3760	punct	", ",Table
R2268	T3764	T3760	nummod	1,Table
R2269	T3765	T3760	punct	),Table
R2270	T3766	T3750	punct	.,interacted
R2271	T3768	T3769	nsubj	This,is
R2272	T3770	T3769	acomp	consistent,is
R2273	T3771	T3770	prep	with,consistent
R2274	T3772	T3773	det	the,screens
R2275	T3773	T3771	pobj	screens,with
R2276	T3774	T3773	compound	library,screens
R2277	T3775	T3776	prep	in,isolated
R2278	T3776	T3773	relcl	isolated,screens
R2279	T3777	T3775	pobj	which,in
R2280	T3778	T3776	nsubjpass	TRIM11,isolated
R2281	T3779	T3776	auxpass	was,isolated
R2282	T3780	T3776	prep	with,isolated
R2283	T3781	T3782	det	the,domain
R2284	T3782	T3780	pobj	domain,with
R2285	T3783	T3782	compound	PST,domain
R2286	T3784	T3780	cc	but,with
R2287	T3785	T3784	neg	not,but
R2288	T3786	T3780	conj	with,with
R2289	T3787	T3788	det	the,peptide
R2290	T3788	T3786	pobj	peptide,with
R2291	T3789	T3790	npadvmod	C,terminal
R2292	T3790	T3788	amod	terminal,peptide
R2293	T3791	T3790	punct	-,terminal
R2294	T3792	T3769	cc	and,is
R2295	T3793	T3769	conj	supports,is
R2296	T3794	T3795	det	the,idea
R2297	T3795	T3793	dobj	idea,supports
R2298	T3796	T3797	mark	that,is
R2299	T3797	T3795	acl	is,idea
R2300	T3798	T3799	det	the,peptide
R2301	T3799	T3797	nsubj	peptide,is
R2302	T3800	T3801	npadvmod	C,terminal
R2303	T3801	T3799	amod	terminal,peptide
R2304	T3802	T3801	punct	-,terminal
R2305	T3803	T3797	neg	not,is
R2306	T3804	T3797	acomp	important,is
R2307	T3805	T3804	prep	for,important
R2308	T3806	T3807	det	the,interaction
R2309	T3807	T3805	pobj	interaction,for
R2310	T3808	T3807	prep	between,interaction
R2311	T3809	T3808	pobj	PAX6,between
R2312	T3810	T3809	cc	and,PAX6
R2313	T3811	T3809	conj	TRIM11,PAX6
R2314	T3812	T3769	punct	.,is
R2315	T3818	T3819	nummod	v,in
R2316	T3829	T3830	advcl	ed,investigated
R2317	T3831	T3832	mark	that,interact
R2318	T3832	T3829	ccomp	interact,ed
R2319	T3833	T3832	nsubj	HOMER3,interact
R2320	T3834	T3833	punct	", ",HOMER3
R2321	T3835	T3833	conj	DNCL1,HOMER3
R2322	T3836	T3835	cc	and,DNCL1
R2323	T3837	T3835	conj	TRIM11,DNCL1
R2324	T3838	T3832	prep	with,interact
R2325	T3839	T3840	det	the,domain
R2326	T3840	T3838	pobj	domain,with
R2327	T3841	T3840	compound	PAX6,domain
R2328	T3842	T3840	compound	PST,domain
R2329	T3843	T3830	punct	", ",investigated
R2330	T3844	T3830	nsubj	we,investigated
R2331	T3845	T3830	advmod	next,investigated
R2332	T3846	T3847	advmod	how,affected
R2333	T3847	T3830	ccomp	affected,investigated
R2334	T3848	T3849	det	the,interactions
R2335	T3849	T3847	nsubjpass	interactions,affected
R2336	T3850	T3847	auxpass	were,affected
R2337	T3851	T3847	agent	by,affected
R2338	T3852	T3853	nummod	three,mutations
R2339	T3853	T3851	pobj	mutations,by
R2340	T3854	T3855	npadvmod	C,terminal
R2341	T3855	T3853	amod	terminal,mutations
R2342	T3856	T3855	punct	-,terminal
R2343	T3857	T3853	compound	PAX6,mutations
R2344	T3858	T3859	dep	that,described
R2345	T3859	T3853	relcl	described,mutations
R2346	T3860	T3859	aux	have,described
R2347	T3861	T3859	auxpass	been,described
R2348	T3862	T3859	advmod	previously,described
R2349	T3863	T3859	prep	in,described
R2350	T3864	T3863	pobj	patients,in
R2351	T3865	T3864	prep	with,patients
R2352	T3866	T3867	amod	ocular,anomalies
R2353	T3867	T3865	pobj	anomalies,with
R2354	T3868	T3830	punct	.,investigated
R2355	T3870	T3871	det	The,mutation
R2356	T3871	T3873	nsubj	mutation,is
R2357	T3872	T3871	amod	first,mutation
R2358	T3874	T3875	det	a,substitution
R2359	T3875	T3873	attr	substitution,is
R2360	T3876	T3875	amod	single,substitution
R2361	T3877	T3875	compound	nucleotide,substitution
R2362	T3878	T3875	appos	1627A,substitution
R2363	T3879	T3880	punct	>,G
R2364	T3880	T3878	prep	G,1627A
R2365	T3881	T3882	dep	that,causes
R2366	T3882	T3875	relcl	causes,substitution
R2367	T3883	T3884	det	a,substitution
R2368	T3884	T3882	dobj	substitution,causes
R2369	T3885	T3884	nmod	glutamine,substitution
R2370	T3886	T3885	prep	to,glutamine
R2371	T3887	T3886	pobj	arginine,to
R2372	T3888	T3889	compound	amino,acid
R2373	T3889	T3884	compound	acid,substitution
R2374	T3890	T3882	prep	in,causes
R2375	T3891	T3892	det	the,codon
R2376	T3892	T3890	pobj	codon,in
R2377	T3893	T3892	amod	last,codon
R2378	T3894	T3892	prep	of,codon
R2379	T3895	T3894	pobj	PAX6,of
R2380	T3896	T3873	punct	.,is
R2381	T3898	T3899	det	This,mutation
R2382	T3899	T3901	nsubjpass	mutation,reported
R2383	T3900	T3899	amod	missense,mutation
R2384	T3902	T3899	punct	(,mutation
R2385	T3903	T3899	appos	Q422R,mutation
R2386	T3904	T3899	punct	),mutation
R2387	T3905	T3901	aux	has,reported
R2388	T3906	T3901	auxpass	been,reported
R2389	T3907	T3901	prep	in,reported
R2390	T3908	T3909	nummod	two,patients
R2391	T3909	T3907	pobj	patients,in
R2392	T3910	T3909	punct	", ",patients
R2393	T3911	T3909	appos	one,patients
R2394	T3912	T3911	acl	affected,one
R2395	T3913	T3912	agent	by,affected
R2396	T3914	T3915	amod	anterior,dysgenesis
R2397	T3915	T3913	pobj	dysgenesis,by
R2398	T3916	T3915	compound	segment,dysgenesis
R2399	T3917	T3915	prep	with,dysgenesis
R2400	T3918	T3919	amod	uveal,ectropion
R2401	T3919	T3917	pobj	ectropion,with
R2402	T3920	T3911	cc	and,one
R2403	T3921	T3911	conj	one,one
R2404	T3922	T3921	prep	with,one
R2405	T3923	T3924	amod	typical,aniridia
R2406	T3924	T3922	pobj	aniridia,with
R2407	T3925	T3924	cc	and,aniridia
R2408	T3926	T3927	amod	foveal,hypoplasia
R2409	T3927	T3924	conj	hypoplasia,aniridia
R2410	T3928	T3929	punct	[,33
R2411	T3929	T3901	parataxis	33,reported
R2412	T3930	T3929	nummod	27,33
R2413	T3931	T3929	punct	",",33
R2414	T3932	T3929	punct	],33
R2415	T3933	T3901	punct	.,reported
R2416	T3935	T3936	det	The,mutation
R2417	T3936	T3938	nsubjpass	mutation,found
R2418	T3937	T3936	amod	second,mutation
R2419	T3939	T3936	punct	(,mutation
R2420	T3940	T3936	appos	1615del10,mutation
R2421	T3941	T3936	punct	),mutation
R2422	T3942	T3938	auxpass	was,found
R2423	T3943	T3938	prep	in,found
R2424	T3944	T3945	det	an,family
R2425	T3945	T3943	pobj	family,in
R2426	T3946	T3945	compound	aniridia,family
R2427	T3947	T3948	punct	[,28
R2428	T3948	T3938	parataxis	28,found
R2429	T3949	T3948	punct	],28
R2430	T3950	T3938	punct	.,found
R2431	T3952	T3953	det	This,deletion
R2432	T3953	T3957	nsubj	deletion,occurs
R2433	T3954	T3955	npadvmod	frame,shifting
R2434	T3955	T3953	amod	shifting,deletion
R2435	T3956	T3955	punct	-,shifting
R2436	T3958	T3959	advmod	just,before
R2437	T3959	T3957	prep	before,occurs
R2438	T3960	T3961	det	the,codon
R2439	T3961	T3959	pobj	codon,before
R2440	T3962	T3961	compound	PAX6,codon
R2441	T3963	T3961	compound	stop,codon
R2442	T3964	T3957	cc	and,occurs
R2443	T3965	T3966	auxpass	is,predicted
R2444	T3966	T3957	conj	predicted,occurs
R2445	T3967	T3968	aux	to,cause
R2446	T3968	T3966	xcomp	cause,predicted
R2447	T3969	T3970	amod	translational,through
R2448	T3970	T3968	dobj	through,cause
R2449	T3971	T3970	compound	read,through
R2450	T3972	T3970	punct	-,through
R2451	T3973	T3970	prep	into,through
R2452	T3974	T3975	det	the,region
R2453	T3975	T3973	pobj	region,into
R2454	T3976	T3975	nummod	3,region
R2455	T3977	T3976	punct	',3
R2456	T3978	T3975	amod	untranslated,region
R2457	T3979	T3968	punct	", ",cause
R2458	T3980	T3968	advcl	generating,cause
R2459	T3981	T3982	det	a,protein
R2460	T3982	T3980	dobj	protein,generating
R2461	T3983	T3984	prep	in,replaced
R2462	T3984	T3982	relcl	replaced,protein
R2463	T3985	T3983	pobj	which,in
R2464	T3986	T3987	det	the,acids
R2465	T3987	T3984	nsubjpass	acids,replaced
R2466	T3988	T3987	amod	last,acids
R2467	T3989	T3987	nummod	5,acids
R2468	T3990	T3987	compound	amino,acids
R2469	T3991	T3987	prep	of,acids
R2470	T3992	T3993	det	the,peptide
R2471	T3993	T3991	pobj	peptide,of
R2472	T3994	T3995	npadvmod	C,terminal
R2473	T3995	T3993	amod	terminal,peptide
R2474	T3996	T3995	punct	-,terminal
R2475	T3997	T3984	auxpass	are,replaced
R2476	T3998	T3984	agent	by,replaced
R2477	T3999	T4000	det	a,extension
R2478	T4000	T3998	pobj	extension,by
R2479	T4001	T4002	nummod	103,acid
R2480	T4002	T4000	compound	acid,extension
R2481	T4003	T4002	compound	amino,acid
R2482	T4004	T4000	punct	-,extension
R2483	T4005	T3957	punct	.,occurs
R2484	T4007	T4008	amod	Affected,individuals
R2485	T4008	T4009	nsubj	individuals,showed
R2486	T4010	T4008	prep	in,individuals
R2487	T4011	T4012	det	this,family
R2488	T4012	T4010	pobj	family,in
R2489	T4013	T4014	amod	unusual,traits
R2490	T4014	T4009	dobj	traits,showed
R2491	T4015	T4014	amod	neurobehavioural,traits
R2492	T4016	T4014	prep	including,traits
R2493	T4017	T4018	amod	impaired,cognition
R2494	T4018	T4016	pobj	cognition,including
R2495	T4019	T4018	amod	social,cognition
R2496	T4020	T4018	cc	and,cognition
R2497	T4021	T4022	amod	poor,inhibition
R2498	T4022	T4018	conj	inhibition,cognition
R2499	T4023	T4022	amod	verbal,inhibition
R2500	T4024	T4025	punct	[,28
R2501	T4025	T4009	parataxis	28,showed
R2502	T4026	T4025	punct	],28
R2503	T4027	T4009	punct	.,showed
R2504	T4029	T4030	compound	MRI,analysis
R2505	T4030	T4031	nsubj	analysis,revealed
R2506	T4032	T4033	amod	grey,matter
R2507	T4033	T4034	compound	matter,abnormalities
R2508	T4034	T4031	dobj	abnormalities,revealed
R2509	T4035	T4034	prep	in,abnormalities
R2510	T4036	T4037	det	the,lobe
R2511	T4037	T4035	pobj	lobe,in
R2512	T4038	T4037	amod	frontal,lobe
R2513	T4039	T4037	punct	", ",lobe
R2514	T4040	T4041	amod	temporal,lobe
R2515	T4041	T4037	conj	lobe,lobe
R2516	T4042	T4041	cc	and,lobe
R2517	T4043	T4041	conj	cerebellum,lobe
R2518	T4044	T4034	punct	", ",abnormalities
R2519	T4045	T4034	cc	and,abnormalities
R2520	T4046	T4047	amod	white,matter
R2521	T4047	T4048	compound	matter,deficits
R2522	T4048	T4034	conj	deficits,abnormalities
R2523	T4049	T4048	prep	in,deficits
R2524	T4050	T4051	det	the,callosum
R2525	T4051	T4049	pobj	callosum,in
R2526	T4052	T4051	compound	corpus,callosum
R2527	T4053	T4054	punct	[,13
R2528	T4054	T4031	parataxis	13,revealed
R2529	T4055	T4054	punct	],13
R2530	T4056	T4031	punct	.,revealed
R2531	T4058	T4059	det	The,mutation
R2532	T4059	T4061	nsubjpass	mutation,reported
R2533	T4060	T4059	amod	third,mutation
R2534	T4062	T4059	appos	1629insT,mutation
R2535	T4063	T4062	punct	(,1629insT
R2536	T4064	T4062	appos	X423L,1629insT
R2537	T4065	T4059	punct	),mutation
R2538	T4066	T4061	aux	has,reported
R2539	T4067	T4061	auxpass	been,reported
R2540	T4068	T4061	prep	in,reported
R2541	T4069	T4070	amod	several,patients
R2542	T4070	T4068	pobj	patients,in
R2543	T4071	T4070	compound	aniridia,patients
R2544	T4072	T4073	punct	[,33
R2545	T4073	T4061	parataxis	33,reported
R2546	T4074	T4073	nummod	11,33
R2547	T4075	T4073	punct	",",33
R2548	T4076	T4073	nummod	12,33
R2549	T4077	T4073	punct	",",33
R2550	T4078	T4073	nummod	27,33
R2551	T4079	T4073	punct	",",33
R2552	T4080	T4073	punct	],33
R2553	T4081	T4061	punct	.,reported
R2554	T4083	T4084	nsubj	Insertion,changes
R2555	T4085	T4083	prep	of,Insertion
R2556	T4086	T4087	det	a,nucleotide
R2557	T4087	T4085	pobj	nucleotide,of
R2558	T4088	T4087	amod	single,nucleotide
R2559	T4089	T4087	compound	T,nucleotide
R2560	T4090	T4083	prep	at,Insertion
R2561	T4091	T4090	pobj	position,at
R2562	T4092	T4091	nummod	1629,position
R2563	T4093	T4094	det	the,codon
R2564	T4094	T4084	dobj	codon,changes
R2565	T4095	T4094	compound	stop,codon
R2566	T4096	T4094	punct	(,codon
R2567	T4097	T4094	appos	TAA,codon
R2568	T4098	T4094	punct	),codon
R2569	T4099	T4084	prep	to,changes
R2570	T4100	T4101	det	a,codon
R2571	T4101	T4099	pobj	codon,to
R2572	T4102	T4101	compound	leucine,codon
R2573	T4103	T4101	punct	(,codon
R2574	T4104	T4101	appos	TTA,codon
R2575	T4105	T4101	punct	),codon
R2576	T4106	T4084	cc	and,changes
R2577	T4107	T4084	conj	generates,changes
R2578	T4108	T4109	det	a,protein
R2579	T4109	T4107	dobj	protein,generates
R2580	T4110	T4111	amod	full,length
R2581	T4111	T4109	compound	length,protein
R2582	T4112	T4109	compound	PAX6,protein
R2583	T4113	T4109	prep	with,protein
R2584	T4114	T4115	det	a,extension
R2585	T4115	T4113	pobj	extension,with
R2586	T4116	T4117	npadvmod	C,terminal
R2587	T4117	T4115	amod	terminal,extension
R2588	T4118	T4117	punct	-,terminal
R2589	T4119	T4120	dep	that,extends
R2590	T4120	T4115	relcl	extends,extension
R2591	T4121	T4120	prep	for,extends
R2592	T4122	T4123	det	a,acids
R2593	T4123	T4121	pobj	acids,for
R2594	T4124	T4123	amod	further,acids
R2595	T4125	T4123	nummod	35,acids
R2596	T4126	T4123	compound	amino,acids
R2597	T4127	T4120	prep	into,extends
R2598	T4128	T4129	det	the,region
R2599	T4129	T4127	pobj	region,into
R2600	T4130	T4129	nummod	3,region
R2601	T4131	T4130	punct	',3
R2602	T4132	T4129	amod	untranslated,region
R2603	T4133	T4084	punct	.,changes
R2604	T4135	T4136	compound	MRI,analysis
R2605	T4136	T4137	nsubj	analysis,revealed
R2606	T4138	T4136	prep	of,analysis
R2607	T4139	T4140	nummod	six,patients
R2608	T4140	T4138	pobj	patients,of
R2609	T4141	T4140	prep	with,patients
R2610	T4142	T4143	det	this,mutation
R2611	T4143	T4141	pobj	mutation,with
R2612	T4144	T4145	amod	variable,defects
R2613	T4145	T4137	dobj	defects,revealed
R2614	T4146	T4145	compound	brain,defects
R2615	T4147	T4145	prep	including,defects
R2616	T4148	T4147	pobj	absence,including
R2617	T4149	T4148	cc	or,absence
R2618	T4150	T4148	conj	hypoplasia,absence
R2619	T4151	T4148	prep	of,absence
R2620	T4152	T4153	det	the,commissure
R2621	T4153	T4151	pobj	commissure,of
R2622	T4154	T4153	amod	anterior,commissure
R2623	T4155	T4153	punct	", ",commissure
R2624	T4156	T4157	amod	pineal,gland
R2625	T4157	T4153	conj	gland,commissure
R2626	T4158	T4157	cc	and,gland
R2627	T4159	T4160	amod	olfactory,bulbs
R2628	T4160	T4157	conj	bulbs,gland
R2629	T4161	T4162	punct	[,12
R2630	T4162	T4137	parataxis	12,revealed
R2631	T4163	T4162	punct	],12
R2632	T4164	T4137	punct	.,revealed
R2633	T4166	T4167	nummod	Two,patients
R2634	T4167	T4168	nsubj	patients,had
R2635	T4168	T4169	ccomp	had,one
R2636	T4170	T4171	amod	temporal,polymicrogyria
R2637	T4171	T4168	dobj	polymicrogyria,had
R2638	T4172	T4169	punct	", ",one
R2639	T4173	T4169	prep	in,one
R2640	T4174	T4173	pobj	association,in
R2641	T4175	T4174	prep	with,association
R2642	T4176	T4175	pobj	epilepsy,with
R2643	T4177	T4178	punct	[,12
R2644	T4178	T4169	parataxis	12,one
R2645	T4179	T4178	punct	],12
R2646	T4180	T4169	punct	.,one
R2647	T4182	T4183	det	The,mutations
R2648	T4183	T4185	nsubjpass	mutations,introduced
R2649	T4184	T4183	nummod	three,mutations
R2650	T4186	T4185	auxpass	were,introduced
R2651	T4187	T4185	prep	into,introduced
R2652	T4188	T4189	det	the,construct
R2653	T4189	T4187	pobj	construct,into
R2654	T4190	T4189	compound	PAX6PST,construct
R2655	T4191	T4185	punct	", ",introduced
R2656	T4192	T4185	cc	and,introduced
R2657	T4193	T4194	amod	pairwise,tests
R2658	T4194	T4195	nsubjpass	tests,carried
R2659	T4195	T4185	conj	carried,introduced
R2660	T4196	T4195	auxpass	were,carried
R2661	T4197	T4195	prt	out,carried
R2662	T4198	T4199	aux	to,investigate
R2663	T4199	T4195	advcl	investigate,carried
R2664	T4200	T4201	det	the,interaction
R2665	T4201	T4199	dobj	interaction,investigate
R2666	T4202	T4201	prep	of,interaction
R2667	T4203	T4204	det	each,protein
R2668	T4204	T4202	pobj	protein,of
R2669	T4205	T4204	compound	mutant,protein
R2670	T4206	T4201	prep	with,interaction
R2671	T4207	T4206	pobj	HOMER3,with
R2672	T4208	T4207	punct	", ",HOMER3
R2673	T4209	T4207	conj	DNCL1,HOMER3
R2674	T4210	T4209	cc	and,DNCL1
R2675	T4211	T4209	conj	TRIM11,DNCL1
R2676	T4212	T4195	punct	.,carried
R2677	T4214	T4215	det	All,mutations
R2678	T4215	T4217	nsubj	mutations,had
R2679	T4216	T4215	nummod	three,mutations
R2680	T4218	T4219	det	a,effect
R2681	T4219	T4217	dobj	effect,had
R2682	T4220	T4219	amod	clear,effect
R2683	T4221	T4219	prep	on,effect
R2684	T4222	T4223	det	the,interactions
R2685	T4223	T4221	pobj	interactions,on
R2686	T4224	T4217	punct	.,had
R2687	T4226	T4227	det	The,mutation
R2688	T4227	T4230	nsubj	mutation,caused
R2689	T4228	T4229	advmod	most,subtle
R2690	T4229	T4227	amod	subtle,mutation
R2691	T4231	T4227	punct	(,mutation
R2692	T4232	T4227	appos	Q422R,mutation
R2693	T4233	T4227	punct	),mutation
R2694	T4234	T4235	det	a,reduction
R2695	T4235	T4230	dobj	reduction,caused
R2696	T4236	T4235	prep	in,reduction
R2697	T4237	T4238	det	the,interaction
R2698	T4238	T4236	pobj	interaction,in
R2699	T4239	T4238	prep	with,interaction
R2700	T4240	T4239	pobj	HOMER3,with
R2701	T4241	T4240	cc	and,HOMER3
R2702	T4242	T4240	conj	DNCL1,HOMER3
R2703	T4243	T4244	punct	(,Table
R2704	T4244	T4230	parataxis	Table,caused
R2705	T4245	T4244	dep	Figure,Table
R2706	T4246	T4245	nummod	3,Figure
R2707	T4247	T4244	punct	", ",Table
R2708	T4248	T4244	nummod	1,Table
R2709	T4249	T4244	punct	),Table
R2710	T4250	T4230	punct	.,caused
R2711	T4252	T4253	det	The,X423L
R2712	T4253	T4259	nsubj	X423L,reduced
R2713	T4254	T4255	npadvmod	C,terminal
R2714	T4255	T4253	amod	terminal,X423L
R2715	T4256	T4255	punct	-,terminal
R2716	T4257	T4253	compound	extension,X423L
R2717	T4258	T4253	compound	mutations,X423L
R2718	T4260	T4253	cc	and,X423L
R2719	T4261	T4262	compound	1615del10,mutations
R2720	T4262	T4253	conj	mutations,X423L
R2721	T4263	T4253	appos	both,X423L
R2722	T4264	T4259	advmod	dramatically,reduced
R2723	T4265	T4259	cc	or,reduced
R2724	T4266	T4259	conj	abolished,reduced
R2725	T4267	T4268	det	the,interaction
R2726	T4268	T4266	dobj	interaction,abolished
R2727	T4269	T4268	prep	with,interaction
R2728	T4270	T4269	pobj	HOMER3,with
R2729	T4271	T4270	cc	and,HOMER3
R2730	T4272	T4270	conj	DNCL1,HOMER3
R2731	T4273	T4274	punct	(,Table
R2732	T4274	T4266	parataxis	Table,abolished
R2733	T4275	T4274	dep	Figure,Table
R2734	T4276	T4275	nummod	3,Figure
R2735	T4277	T4274	punct	", ",Table
R2736	T4278	T4274	nummod	1,Table
R2737	T4279	T4274	punct	),Table
R2738	T4280	T4259	punct	.,reduced
R2739	T4282	T4283	nsubj	None,affected
R2740	T4284	T4282	prep	of,None
R2741	T4285	T4286	det	the,mutations
R2742	T4286	T4284	pobj	mutations,of
R2743	T4287	T4286	nummod	three,mutations
R2744	T4288	T4289	det	the,interaction
R2745	T4289	T4283	dobj	interaction,affected
R2746	T4290	T4289	prep	with,interaction
R2747	T4291	T4290	pobj	TRIM11,with
R2748	T4292	T4293	dep	which,is
R2749	T4293	T4283	advcl	is,affected
R2750	T4294	T4293	advmod	again,is
R2751	T4295	T4293	acomp	consistent,is
R2752	T4296	T4295	prep	with,consistent
R2753	T4297	T4298	det	the,hypothesis
R2754	T4298	T4296	pobj	hypothesis,with
R2755	T4299	T4300	mark	that,interacts
R2756	T4300	T4298	acl	interacts,hypothesis
R2757	T4301	T4300	nsubj	TRIM11,interacts
R2758	T4302	T4300	prep	with,interacts
R2759	T4303	T4304	det	a,part
R2760	T4304	T4302	pobj	part,with
R2761	T4305	T4306	advmod	more,terminal
R2762	T4306	T4304	amod	terminal,part
R2763	T4307	T4306	npadvmod	N,terminal
R2764	T4308	T4306	punct	-,terminal
R2765	T4309	T4304	prep	of,part
R2766	T4310	T4311	det	the,domain
R2767	T4311	T4309	pobj	domain,of
R2768	T4312	T4311	compound	PST,domain
R2769	T4313	T4283	punct	.,affected
R5301	T8300	T8299	prep	of,Characterisation
R5302	T8301	T8302	det	the,peptide
R5303	T8302	T8300	pobj	peptide,of
R5304	T8303	T8302	nmod	PAX6,peptide
R5305	T8304	T8305	npadvmod	C,terminal
R5306	T8305	T8302	amod	terminal,peptide
R5307	T8306	T8305	punct	-,terminal
R5308	T8307	T8299	punct	.,Characterisation
R5309	T8309	T8310	punct	(,a
R5310	T8310	T8311	meta	a,alignment
R5311	T8312	T8310	punct	),a
R5312	T8313	T8311	compound	CLUSTAL,alignment
R5313	T8314	T8311	prep	of,alignment
R5314	T8315	T8316	det	the,acids
R5315	T8316	T8314	pobj	acids,of
R5316	T8317	T8316	amod	terminal,acids
R5317	T8318	T8316	nummod	42,acids
R5318	T8319	T8316	compound	amino,acids
R5319	T8320	T8316	prep	of,acids
R5320	T8321	T8320	pobj	PAX6,of
R5321	T8322	T8316	prep	from,acids
R5322	T8323	T8324	amod	diverse,species
R5323	T8324	T8322	pobj	species,from
R5324	T8325	T8311	punct	.,alignment
R5325	T8327	T8328	punct	(,indicates
R5326	T8328	T8331	ccomp	indicates,indicates
R5327	T8329	T8328	nsubj	*,indicates
R5328	T8330	T8328	punct	),indicates
R5329	T8332	T8333	amod	invariant,residues
R5330	T8333	T8328	dobj	residues,indicates
R5331	T8334	T8328	punct	", ",indicates
R5332	T8335	T8328	punct	(,indicates
R5333	T8336	T8331	nsubj	:,indicates
R5334	T8337	T8331	punct	),indicates
R5335	T8338	T8339	advmod	highly,similar
R5336	T8339	T8340	amod	similar,substitutions
R5337	T8340	T8331	dobj	substitutions,indicates
R5338	T8341	T8331	cc	and,indicates
R5339	T8342	T8343	punct	(,indicates
R5340	T8343	T8331	conj	indicates,indicates
R5341	T8344	T8343	nsubj	.,indicates
R5342	T8345	T8343	punct	),indicates
R5343	T8346	T8347	advmod	moderately,similar
R5344	T8347	T8348	amod	similar,substitutions
R5345	T8348	T8343	dobj	substitutions,indicates
R5346	T8349	T8343	punct	.,indicates
R5347	T8351	T8352	punct	(,b
R5348	T8352	T8353	meta	b,predicts
R5349	T8354	T8352	punct	),b
R5350	T8355	T8356	amod	Secondary,analysis
R5351	T8356	T8353	nsubj	analysis,predicts
R5352	T8357	T8356	compound	structure,analysis
R5353	T8358	T8356	prep	of,analysis
R5354	T8359	T8360	det	the,acids
R5355	T8360	T8358	pobj	acids,of
R5356	T8361	T8362	advmod	highly,conserved
R5357	T8362	T8360	amod	conserved,acids
R5358	T8363	T8360	amod	terminal,acids
R5359	T8364	T8360	nummod	28,acids
R5360	T8365	T8360	compound	amino,acids
R5361	T8366	T8367	det	a,sheet
R5362	T8367	T8353	dobj	sheet,predicts
R5363	T8368	T8367	amod	single,sheet
R5364	T8369	T8367	compound	beta,sheet
R5365	T8370	T8371	punct	(,arrow
R5366	T8371	T8367	parataxis	arrow,sheet
R5367	T8372	T8371	punct	),arrow
R5368	T8373	T8353	prep	in,predicts
R5369	T8374	T8375	det	the,peptide
R5370	T8375	T8373	pobj	peptide,in
R5371	T8376	T8375	compound	SVPVQ,peptide
R5372	T8377	T8353	punct	.,predicts
R5373	T8379	T8380	punct	(,c
R5374	T8380	T8381	meta	c,predicted
R5375	T8382	T8380	punct	),c
R5376	T8383	T8384	advmod	When,added
R5377	T8384	T8381	advcl	added,predicted
R5378	T8385	T8386	nummod	4,residues
R5379	T8386	T8384	nsubjpass	residues,added
R5380	T8387	T8384	auxpass	are,added
R5381	T8388	T8384	prep	in,added
R5382	T8389	T8390	det	the,direction
R5383	T8390	T8388	pobj	direction,in
R5384	T8391	T8392	npadvmod	N,terminal
R5385	T8392	T8390	amod	terminal,direction
R5386	T8393	T8392	punct	-,terminal
R5387	T8394	T8381	punct	", ",predicted
R5388	T8395	T8396	nummod	2,sheets
R5389	T8396	T8381	nsubjpass	sheets,predicted
R5390	T8397	T8396	compound	beta,sheets
R5391	T8398	T8381	auxpass	are,predicted
R5392	T8399	T8381	advmod	now,predicted
R5393	T8400	T8381	punct	.,predicted
R5394	T8468	T8469	amod	Semi-quantitative,analysis
R5395	T8470	T8469	compound	PCR,analysis
R5396	T8471	T8469	prep	of,analysis
R5397	T8472	T8471	pobj	Homer3,of
R5398	T8473	T8472	punct	", ",Homer3
R5399	T8474	T8472	conj	Dncl1,Homer3
R5400	T8475	T8474	punct	", ",Dncl1
R5401	T8476	T8474	conj	Trim11,Dncl1
R5402	T8477	T8476	cc	and,Trim11
R5403	T8478	T8476	conj	Pax6,Trim11
R5404	T8479	T8469	prep	in,analysis
R5405	T8480	T8481	det	the,library
R5406	T8481	T8479	pobj	library,in
R5407	T8482	T8483	compound	mouse,brain
R5408	T8483	T8481	compound	brain,library
R5409	T8484	T8481	compound	cDNA,library
R5410	T8485	T8469	punct	.,analysis
R5411	T8487	T8488	compound	Library,cDNA
R5412	T8488	T8489	nsubjpass	cDNA,amplified
R5413	T8490	T8489	auxpass	was,amplified
R5414	T8491	T8489	prep	with,amplified
R5415	T8492	T8491	pobj	primers,with
R5416	T8493	T8492	amod	specific,primers
R5417	T8494	T8493	prep	for,specific
R5418	T8495	T8494	pobj	Pax6,for
R5419	T8496	T8497	punct	(,bp
R5420	T8497	T8495	parataxis	bp,Pax6
R5421	T8498	T8497	nummod	600,bp
R5422	T8499	T8497	punct	),bp
R5423	T8500	T8495	punct	", ",Pax6
R5424	T8501	T8495	conj	Homer3,Pax6
R5425	T8502	T8503	punct	(,band
R5426	T8503	T8501	parataxis	band,Homer3
R5427	T8504	T8505	nummod	485,bp
R5428	T8505	T8503	compound	bp,band
R5429	T8506	T8503	punct	),band
R5430	T8507	T8501	punct	", ",Homer3
R5431	T8508	T8501	conj	Dncl1,Homer3
R5432	T8509	T8510	punct	(,bp
R5433	T8510	T8508	parataxis	bp,Dncl1
R5434	T8511	T8510	nummod	485,bp
R5435	T8512	T8510	punct	),bp
R5436	T8513	T8508	cc	and,Dncl1
R5437	T8514	T8508	conj	Trim11,Dncl1
R5438	T8515	T8516	punct	(,bp
R5439	T8516	T8514	parataxis	bp,Trim11
R5440	T8517	T8516	nummod	609,bp
R5441	T8518	T8516	punct	),bp
R5442	T8519	T8489	prep	for,amplified
R5443	T8520	T8521	nummod	20,cycles
R5444	T8521	T8519	pobj	cycles,for
R5445	T8522	T8520	punct	", ",20
R5446	T8523	T8520	conj	25,20
R5447	T8524	T8523	punct	", ",25
R5448	T8525	T8523	conj	30,25
R5449	T8526	T8525	cc	or,30
R5450	T8527	T8525	conj	35,30
R5451	T8528	T8489	punct	.,amplified
R5452	T8530	T8531	nsubjpass	Atp5a1,included
R5453	T8532	T8533	punct	(,bp
R5454	T8533	T8530	parataxis	bp,Atp5a1
R5455	T8534	T8533	nummod	415,bp
R5456	T8535	T8533	punct	),bp
R5457	T8536	T8530	cc	and,Atp5a1
R5458	T8537	T8530	conj	Gapdh,Atp5a1
R5459	T8538	T8539	punct	(,bp
R5460	T8539	T8537	parataxis	bp,Gapdh
R5461	T8540	T8539	nummod	450,bp
R5462	T8541	T8539	punct	),bp
R5463	T8542	T8530	punct	", ",Atp5a1
R5464	T8543	T8544	dep	which,expressed
R5465	T8544	T8530	relcl	expressed,Atp5a1
R5466	T8545	T8544	auxpass	are,expressed
R5467	T8546	T8544	advmod	highly,expressed
R5468	T8547	T8544	prep	in,expressed
R5469	T8548	T8549	det	the,brain
R5470	T8549	T8547	pobj	brain,in
R5471	T8550	T8531	punct	", ",included
R5472	T8551	T8531	auxpass	are,included
R5473	T8552	T8531	prep	for,included
R5474	T8553	T8552	pobj	comparison,for
R5475	T8554	T8531	punct	.,included
R5476	T8556	T8557	nsubj	M,indicates
R5477	T8557	T8558	ccomp	indicates,indicated
R5478	T8559	T8560	det	the,marker
R5479	T8560	T8557	dobj	marker,indicates
R5480	T8561	T8562	nmod	Φ×,HaeIII
R5481	T8562	T8560	compound	HaeIII,marker
R5482	T8563	T8562	nummod	174,HaeIII
R5483	T8564	T8565	compound	DNA,size
R5484	T8565	T8560	compound	size,marker
R5485	T8566	T8558	punct	;,indicated
R5486	T8567	T8568	det	the,positions
R5487	T8568	T8558	nsubjpass	positions,indicated
R5488	T8569	T8568	prep	of,positions
R5489	T8570	T8571	det	the,bands
R5490	T8571	T8569	pobj	bands,of
R5491	T8572	T8573	nummod	603,bp
R5492	T8573	T8571	nmod	bp,bands
R5493	T8574	T8573	cc	and,bp
R5494	T8575	T8576	nummod	310,bp
R5495	T8576	T8573	conj	bp,bp
R5496	T8577	T8571	compound	marker,bands
R5497	T8578	T8558	auxpass	are,indicated
R5498	T8579	T8558	punct	.,indicated
R5499	T8632	T8633	compound	LacZ,activation
R5500	T8634	T8635	compound	reporter,gene
R5501	T8635	T8633	compound	gene,activation
R5502	T8636	T8633	prep	in,activation
R5503	T8637	T8638	amod	pairwise,tests
R5504	T8638	T8636	pobj	tests,in
R5505	T8639	T8633	punct	.,activation
R5506	T8641	T8642	compound	pPC86,constructs
R5507	T8642	T8643	nsubjpass	constructs,shown
R5508	T8644	T8643	auxpass	are,shown
R5509	T8645	T8643	prep	across,shown
R5510	T8646	T8647	det	the,top
R5511	T8647	T8645	pobj	top,across
R5512	T8648	T8643	punct	", ",shown
R5513	T8649	T8643	cc	and,shown
R5514	T8650	T8651	compound	pDBLeu,constructs
R5515	T8651	T8652	nsubjpass	constructs,shown
R5516	T8652	T8643	conj	shown,shown
R5517	T8653	T8652	auxpass	are,shown
R5518	T8654	T8652	prep	down,shown
R5519	T8655	T8656	det	the,side
R5520	T8656	T8654	pobj	side,down
R5521	T8657	T8658	amod	right,hand
R5522	T8658	T8656	compound	hand,side
R5523	T8659	T8652	punct	.,shown
R5524	T8662	T8661	punct	", ",PAX6PST
R5525	T8663	T8664	compound	PAX6,domain
R5526	T8664	T8661	appos	domain,PAX6PST
R5527	T8665	T8664	compound	PST,domain
R5528	T8666	T8661	punct	;,PAX6PST
R5529	T8667	T8668	compound	PAX6PST,Q422R
R5530	T8668	T8661	appos	Q422R,PAX6PST
R5531	T8669	T8668	punct	/,Q422R
R5532	T8670	T8668	punct	", ",Q422R
R5533	T8671	T8672	compound	PAX6,domain
R5534	T8672	T8668	appos	domain,Q422R
R5535	T8673	T8672	compound	PST,domain
R5536	T8674	T8672	prep	with,domain
R5537	T8675	T8676	det	the,mutation
R5538	T8676	T8674	pobj	mutation,with
R5539	T8677	T8676	compound	Q422R,mutation
R5540	T8678	T8661	punct	;,PAX6PST
R5541	T8679	T8680	compound	PAX6PST,X423L
R5542	T8680	T8661	appos	X423L,PAX6PST
R5543	T8681	T8680	punct	/,X423L
R5544	T8682	T8680	punct	", ",X423L
R5545	T8683	T8684	compound	PAX6,domain
R5546	T8684	T8680	appos	domain,X423L
R5547	T8685	T8684	compound	PST,domain
R5548	T8686	T8684	prep	with,domain
R5549	T8687	T8688	det	the,mutation
R5550	T8688	T8686	pobj	mutation,with
R5551	T8689	T8688	compound	X423L,mutation
R5552	T8690	T8661	punct	", ",PAX6PST
R5553	T8691	T8692	compound	PAX6PST,1615del10
R5554	T8692	T8661	appos	1615del10,PAX6PST
R5555	T8693	T8692	punct	/,1615del10
R5556	T8694	T8692	punct	", ",1615del10
R5557	T8695	T8696	compound	PAX6,domain
R5558	T8696	T8692	appos	domain,1615del10
R5559	T8697	T8696	compound	PST,domain
R5560	T8698	T8696	prep	with,domain
R5561	T8699	T8700	det	the,mutation
R5562	T8700	T8698	pobj	mutation,with
R5563	T8701	T8700	nummod	1615,mutation
R5564	T8702	T8700	compound	del10,mutation
R5565	T8703	T8661	punct	;,PAX6PST
R5566	T8704	T8705	compound	PAX6PST,CT
R5567	T8705	T8661	appos	CT,PAX6PST
R5568	T8706	T8705	punct	-,CT
R5569	T8707	T8705	punct	", ",CT
R5570	T8708	T8709	compound	PAX6,PST
R5571	T8709	T8710	compound	PST,domain
R5572	T8710	T8705	appos	domain,CT
R5573	T8711	T8710	cc	minus,domain
R5574	T8712	T8713	det	the,peptide
R5575	T8713	T8710	conj	peptide,domain
R5576	T8714	T8715	npadvmod	C,terminal
R5577	T8715	T8713	amod	terminal,peptide
R5578	T8716	T8715	punct	-,terminal
R5579	T8717	T8661	punct	.,PAX6PST
R5580	T8719	T8720	punct	',is
R5581	T8721	T8722	amod	Truncated,HOMER3
R5582	T8722	T8720	nsubj	HOMER3,is
R5583	T8723	T8720	punct	',is
R5584	T8724	T8720	attr	HOMER3,is
R5585	T8725	T8724	acl	lacking,HOMER3
R5586	T8726	T8727	det	the,acids
R5587	T8727	T8725	dobj	acids,lacking
R5588	T8728	T8729	npadvmod	N,terminal
R5589	T8729	T8727	amod	terminal,acids
R5590	T8730	T8729	punct	-,terminal
R5591	T8731	T8727	nummod	70,acids
R5592	T8732	T8727	compound	amino,acids
R5593	T8733	T8720	punct	.,is
R5594	T8735	T8736	nummod	Five,strains
R5595	T8736	T8738	nsubjpass	strains,shown
R5596	T8737	T8736	compound	control,strains
R5597	T8739	T8738	auxpass	are,shown
R5598	T8740	T8738	prep	for,shown
R5599	T8741	T8740	pobj	comparison,for
R5600	T8742	T8743	punct	(,left
R5601	T8743	T8738	parataxis	left,shown
R5602	T8744	T8743	punct	),left
R5603	T8745	T8738	punct	.,shown
R5604	T8747	T8748	nsubj	These,range
R5605	T8749	T8748	prep	from,range
R5606	T8750	T8749	pobj	non-interactor,from
R5607	T8751	T8752	punct	(,A
R5608	T8752	T8750	parataxis	A,non-interactor
R5609	T8753	T8752	punct	),A
R5610	T8754	T8749	prep	to,from
R5611	T8755	T8756	amod	strong,interactor
R5612	T8756	T8754	pobj	interactor,to
R5613	T8757	T8758	punct	(,E
R5614	T8758	T8756	parataxis	E,interactor
R5615	T8759	T8758	punct	),E
R5616	T8760	T8748	punct	.,range
R5617	T8837	T8838	amod	Pairwise,tests
R5618	T8839	T8838	compound	interaction,tests
R5619	T8840	T8838	prep	between,tests
R5620	T8841	T8842	amod	normal,constructs
R5621	T8842	T8840	pobj	constructs,between
R5622	T8843	T8841	cc	and,normal
R5623	T8844	T8841	conj	mutant,normal
R5624	T8845	T8842	compound	PAX6,constructs
R5625	T8846	T8842	cc	and,constructs
R5626	T8847	T8842	conj	HOMER3,constructs
R5627	T8848	T8847	punct	", ",HOMER3
R5628	T8849	T8847	conj	DNCL1,HOMER3
R5629	T8850	T8849	cc	and,DNCL1
R5630	T8851	T8849	conj	TRIM11,DNCL1
R5631	T8852	T8838	punct	.,tests
R5632	T8855	T8854	punct	: ,+++
R5633	T8856	T8857	amod	strong,interaction
R5634	T8857	T8854	appos	interaction,+++
R5635	T8858	T8854	punct	;,+++
R5636	T8859	T8854	appos	++,+++
R5637	T8860	T8859	punct	: ,++
R5638	T8861	T8862	amod	moderate,interaction
R5639	T8862	T8859	appos	interaction,++
R5640	T8863	T8854	punct	;,+++
R5641	T8864	T8854	appos	+,+++
R5642	T8865	T8864	punct	: ,+
R5643	T8866	T8867	amod	weak,interaction
R5644	T8867	T8864	appos	interaction,+
R5645	T8868	T8854	punct	;,+++
R5646	T8869	T8870	punct	(,)
R5647	T8870	T8854	appos	),+++
R5648	T8871	T8870	punct	+,)
R5649	T8872	T8873	amod	borderline,interaction
R5650	T8873	T8870	appos	interaction,)
R5651	T8874	T8873	prep	with,interaction
R5652	T8875	T8876	nummod	one,reporters
R5653	T8876	T8874	pobj	reporters,with
R5654	T8877	T8875	cc	or,one
R5655	T8878	T8875	conj	two,one
R5656	T8879	T8876	acl	activated,reporters
R5657	T8880	T8879	prep	at,activated
R5658	T8881	T8882	advmod	very,low
R5659	T8882	T8883	amod	low,levels
R5660	T8883	T8880	pobj	levels,at
R5661	T8884	T8854	punct	;,+++
R5662	T8885	T8854	appos	0,+++
R5663	T8886	T8885	punct	: ,0
R5664	T8887	T8888	det	no,interaction
R5665	T8888	T8885	appos	interaction,0
R5666	T8889	T8854	punct	.,+++
R5667	T8892	T8891	punct	", ",PAX6PST
R5668	T8893	T8894	compound	PAX6,domain
R5669	T8894	T8891	appos	domain,PAX6PST
R5670	T8895	T8894	compound	PST,domain
R5671	T8896	T8891	punct	;,PAX6PST
R5672	T8897	T8891	appos	PAX6CTP,PAX6PST
R5673	T8898	T8897	punct	", ",PAX6CTP
R5674	T8899	T8900	nmod	PAX6,peptide
R5675	T8900	T8897	appos	peptide,PAX6CTP
R5676	T8901	T8902	npadvmod	C,terminal
R5677	T8902	T8900	amod	terminal,peptide
R5678	T8903	T8902	punct	-,terminal
R5679	T8904	T8891	punct	;,PAX6PST
R5680	T8905	T8906	compound	PAX6PST,CT
R5681	T8906	T8891	appos	CT,PAX6PST
R5682	T8907	T8906	punct	-,CT
R5683	T8908	T8906	punct	", ",CT
R5684	T8909	T8910	compound	PAX6,domain
R5685	T8910	T8906	appos	domain,CT
R5686	T8911	T8910	compound	PST,domain
R5687	T8912	T8910	cc	minus,domain
R5688	T8913	T8914	det	the,peptide
R5689	T8914	T8910	conj	peptide,domain
R5690	T8915	T8916	npadvmod	C,terminal
R5691	T8916	T8914	amod	terminal,peptide
R5692	T8917	T8916	punct	-,terminal
R5693	T8918	T8891	punct	;,PAX6PST
R5694	T8919	T8920	compound	PAX6PST,Q422R
R5695	T8920	T8891	appos	Q422R,PAX6PST
R5696	T8921	T8920	punct	/,Q422R
R5697	T8922	T8920	punct	", ",Q422R
R5698	T8923	T8924	compound	PAX6,domain
R5699	T8924	T8920	appos	domain,Q422R
R5700	T8925	T8924	compound	PST,domain
R5701	T8926	T8924	prep	with,domain
R5702	T8927	T8928	det	the,mutation
R5703	T8928	T8926	pobj	mutation,with
R5704	T8929	T8928	compound	Q422R,mutation
R5705	T8930	T8891	punct	;,PAX6PST
R5706	T8931	T8932	compound	PAX6PST,X423L
R5707	T8932	T8891	appos	X423L,PAX6PST
R5708	T8933	T8932	punct	/,X423L
R5709	T8934	T8932	punct	", ",X423L
R5710	T8935	T8936	compound	PAX6,domain
R5711	T8936	T8932	appos	domain,X423L
R5712	T8937	T8936	compound	PST,domain
R5713	T8938	T8936	prep	with,domain
R5714	T8939	T8940	det	the,mutation
R5715	T8940	T8938	pobj	mutation,with
R5716	T8941	T8940	compound	X423L,mutation
R5717	T8942	T8891	punct	", ",PAX6PST
R5718	T8943	T8944	compound	PAX6PST,1615del
R5719	T8944	T8891	appos	1615del,PAX6PST
R5720	T8945	T8944	punct	/,1615del
R5721	T8946	T8944	punct	", ",1615del
R5722	T8947	T8948	compound	PAX6,domain
R5723	T8948	T8944	appos	domain,1615del
R5724	T8949	T8948	compound	PST,domain
R5725	T8950	T8948	prep	with,domain
R5726	T8951	T8952	det	the,mutation
R5727	T8952	T8950	pobj	mutation,with
R5728	T8953	T8952	nummod	1615,mutation
R5729	T8954	T8952	compound	del10,mutation
R5730	T8955	T8891	punct	.,PAX6PST
R5731	T8957	T8958	compound	HOMER3,FL
R5732	T8959	T8958	punct	-,FL
R5733	T8960	T8958	punct	", ",FL
R5734	T8961	T8962	nmod	HOMER3,clone
R5735	T8962	T8958	appos	clone,FL
R5736	T8963	T8964	amod	full,length
R5737	T8964	T8962	compound	length,clone
R5738	T8965	T8964	punct	-,length
R5739	T8966	T8958	punct	;,FL
R5740	T8967	T8968	compound	HOMER3,Tr
R5741	T8968	T8958	appos	Tr,FL
R5742	T8969	T8968	punct	-,Tr
R5743	T8970	T8968	punct	", ",Tr
R5744	T8971	T8972	amod	truncated,clone
R5745	T8972	T8968	appos	clone,Tr
R5746	T8973	T8972	compound	HOMER3,clone
R5747	T8974	T8972	acl	lacking,clone
R5748	T8975	T8976	det	the,acids
R5749	T8976	T8974	dobj	acids,lacking
R5750	T8977	T8978	npadvmod	N,terminal
R5751	T8978	T8976	amod	terminal,acids
R5752	T8979	T8978	punct	-,terminal
R5753	T8980	T8976	nummod	70,acids
R5754	T8981	T8976	compound	amino,acids
R5755	T8982	T8958	punct	.,FL

PMC:1208879 / 5685-17652 JSONTXT 4 Projects

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PMC:1208879 / 5685-17652 JSON TXT 4 Projects