PMC:1208879 / 10630-11202 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T2968 0-572 sentence denotes Figure 2 Semi-quantitative PCR analysis of Homer3, Dncl1, Trim11 and Pax6 in the mouse brain cDNA library. Library cDNA was amplified with primers specific for Pax6 (600 bp), Homer3 (485 bp band), Dncl1 (485 bp) and Trim11 (609 bp) for 20, 25, 30 or 35 cycles. Atp5a1 (415 bp) and Gapdh (450 bp), which are highly expressed in the brain, are included for comparison. M indicates the Φ×174 HaeIII DNA size marker; the positions of the 603 bp and 310 bp marker bands are indicated. We concluded that Homer3, Dncl1 and Trim11 clones were not highly abundant in the library.
T8468 11-28 JJ denotes Semi-quantitative
T8470 29-32 NN denotes PCR
T8469 33-41 NN denotes analysis
T8471 42-44 IN denotes of
T8472 45-51 NN denotes Homer3
T8473 51-53 , denotes ,
T8474 53-58 NN denotes Dncl1
T8475 58-60 , denotes ,
T8476 60-66 NN denotes Trim11
T8477 67-70 CC denotes and
T8478 71-75 NN denotes Pax6
T8479 76-78 IN denotes in
T8480 79-82 DT denotes the
T8482 83-88 NN denotes mouse
T8483 89-94 NN denotes brain
T8484 95-99 NN denotes cDNA
T8481 100-107 NN denotes library
T8485 107-108 . denotes .
T8486 108-262 sentence denotes Library cDNA was amplified with primers specific for Pax6 (600 bp), Homer3 (485 bp band), Dncl1 (485 bp) and Trim11 (609 bp) for 20, 25, 30 or 35 cycles.
T8487 109-116 NN denotes Library
T8488 117-121 NN denotes cDNA
T8490 122-125 VBD denotes was
T8489 126-135 VBN denotes amplified
T8491 136-140 IN denotes with
T8492 141-148 NNS denotes primers
T8493 149-157 JJ denotes specific
T8494 158-161 IN denotes for
T8495 162-166 NN denotes Pax6
T8496 167-168 -LRB- denotes (
T8498 168-171 CD denotes 600
T8497 172-174 NN denotes bp
T8499 174-175 -RRB- denotes )
T8500 175-177 , denotes ,
T8501 177-183 NN denotes Homer3
T8502 184-185 -LRB- denotes (
T8504 185-188 CD denotes 485
T8505 189-191 NN denotes bp
T8503 192-196 NN denotes band
T8506 196-197 -RRB- denotes )
T8507 197-199 , denotes ,
T8508 199-204 NN denotes Dncl1
T8509 205-206 -LRB- denotes (
T8511 206-209 CD denotes 485
T8510 210-212 NN denotes bp
T8512 212-213 -RRB- denotes )
T8513 214-217 CC denotes and
T8514 218-224 NN denotes Trim11
T8515 225-226 -LRB- denotes (
T8517 226-229 CD denotes 609
T8516 230-232 NN denotes bp
T8518 232-233 -RRB- denotes )
T8519 234-237 IN denotes for
T8520 238-240 CD denotes 20
T8522 240-242 , denotes ,
T8523 242-244 CD denotes 25
T8524 244-246 , denotes ,
T8525 246-248 CD denotes 30
T8526 249-251 CC denotes or
T8527 252-254 CD denotes 35
T8521 255-261 NNS denotes cycles
T8528 261-262 . denotes .
T8529 262-368 sentence denotes Atp5a1 (415 bp) and Gapdh (450 bp), which are highly expressed in the brain, are included for comparison.
T8530 263-269 NN denotes Atp5a1
T8532 270-271 -LRB- denotes (
T8534 271-274 CD denotes 415
T8533 275-277 NN denotes bp
T8535 277-278 -RRB- denotes )
T8536 279-282 CC denotes and
T8537 283-288 NN denotes Gapdh
T8538 289-290 -LRB- denotes (
T8540 290-293 CD denotes 450
T8539 294-296 NN denotes bp
T8541 296-297 -RRB- denotes )
T8542 297-299 , denotes ,
T8543 299-304 WDT denotes which
T8545 305-308 VBP denotes are
T8546 309-315 RB denotes highly
T8544 316-325 VBN denotes expressed
T8547 326-328 IN denotes in
T8548 329-332 DT denotes the
T8549 333-338 NN denotes brain
T8550 338-340 , denotes ,
T8551 340-343 VBP denotes are
T8531 344-352 VBN denotes included
T8552 353-356 IN denotes for
T8553 357-367 NN denotes comparison
T8554 367-368 . denotes .
T8555 368-481 sentence denotes M indicates the Φ×174 HaeIII DNA size marker; the positions of the 603 bp and 310 bp marker bands are indicated.
T8556 369-370 NN denotes M
T8557 371-380 VBZ denotes indicates
T8559 381-384 DT denotes the
T8561 385-387 NN denotes Φ×
T8563 387-390 CD denotes 174
T8562 391-397 NN denotes HaeIII
T8564 398-401 NN denotes DNA
T8565 402-406 NN denotes size
T8560 407-413 NN denotes marker
T8566 413-414 : denotes ;
T8567 415-418 DT denotes the
T8568 419-428 NNS denotes positions
T8569 429-431 IN denotes of
T8570 432-435 DT denotes the
T8572 436-439 CD denotes 603
T8573 440-442 NN denotes bp
T8574 443-446 CC denotes and
T8575 447-450 CD denotes 310
T8576 451-453 NN denotes bp
T8577 454-460 NN denotes marker
T8571 461-466 NNS denotes bands
T8578 467-470 VBP denotes are
T8558 471-480 VBN denotes indicated
T8579 480-481 . denotes .
T2969 482-484 PRP denotes We
T2970 485-494 VBD denotes concluded
T2971 495-499 IN denotes that
T2973 500-506 NN denotes Homer3
T2975 506-508 , denotes ,
T2976 508-513 NN denotes Dncl1
T2977 514-517 CC denotes and
T2978 518-524 NN denotes Trim11
T2974 525-531 NNS denotes clones
T2972 532-536 VBD denotes were
T2979 537-540 RB denotes not
T2980 541-547 RB denotes highly
T2981 548-556 JJ denotes abundant
T2982 557-559 IN denotes in
T2983 560-563 DT denotes the
T2984 564-571 NN denotes library
T2985 571-572 . denotes .
R1867 T2969 T2970 nsubj We,concluded
R1868 T2971 T2972 mark that,were
R1869 T2972 T2970 ccomp were,concluded
R1870 T2973 T2974 nmod Homer3,clones
R1871 T2974 T2972 nsubj clones,were
R1872 T2975 T2973 punct ", ",Homer3
R1873 T2976 T2973 conj Dncl1,Homer3
R1874 T2977 T2976 cc and,Dncl1
R1875 T2978 T2976 conj Trim11,Dncl1
R1876 T2979 T2972 neg not,were
R1877 T2980 T2981 advmod highly,abundant
R1878 T2981 T2972 acomp abundant,were
R1879 T2982 T2972 prep in,were
R1880 T2983 T2984 det the,library
R1881 T2984 T2982 pobj library,in
R1882 T2985 T2970 punct .,concluded
R5394 T8468 T8469 amod Semi-quantitative,analysis
R5395 T8470 T8469 compound PCR,analysis
R5396 T8471 T8469 prep of,analysis
R5397 T8472 T8471 pobj Homer3,of
R5398 T8473 T8472 punct ", ",Homer3
R5399 T8474 T8472 conj Dncl1,Homer3
R5400 T8475 T8474 punct ", ",Dncl1
R5401 T8476 T8474 conj Trim11,Dncl1
R5402 T8477 T8476 cc and,Trim11
R5403 T8478 T8476 conj Pax6,Trim11
R5404 T8479 T8469 prep in,analysis
R5405 T8480 T8481 det the,library
R5406 T8481 T8479 pobj library,in
R5407 T8482 T8483 compound mouse,brain
R5408 T8483 T8481 compound brain,library
R5409 T8484 T8481 compound cDNA,library
R5410 T8485 T8469 punct .,analysis
R5411 T8487 T8488 compound Library,cDNA
R5412 T8488 T8489 nsubjpass cDNA,amplified
R5413 T8490 T8489 auxpass was,amplified
R5414 T8491 T8489 prep with,amplified
R5415 T8492 T8491 pobj primers,with
R5416 T8493 T8492 amod specific,primers
R5417 T8494 T8493 prep for,specific
R5418 T8495 T8494 pobj Pax6,for
R5419 T8496 T8497 punct (,bp
R5420 T8497 T8495 parataxis bp,Pax6
R5421 T8498 T8497 nummod 600,bp
R5422 T8499 T8497 punct ),bp
R5423 T8500 T8495 punct ", ",Pax6
R5424 T8501 T8495 conj Homer3,Pax6
R5425 T8502 T8503 punct (,band
R5426 T8503 T8501 parataxis band,Homer3
R5427 T8504 T8505 nummod 485,bp
R5428 T8505 T8503 compound bp,band
R5429 T8506 T8503 punct ),band
R5430 T8507 T8501 punct ", ",Homer3
R5431 T8508 T8501 conj Dncl1,Homer3
R5432 T8509 T8510 punct (,bp
R5433 T8510 T8508 parataxis bp,Dncl1
R5434 T8511 T8510 nummod 485,bp
R5435 T8512 T8510 punct ),bp
R5436 T8513 T8508 cc and,Dncl1
R5437 T8514 T8508 conj Trim11,Dncl1
R5438 T8515 T8516 punct (,bp
R5439 T8516 T8514 parataxis bp,Trim11
R5440 T8517 T8516 nummod 609,bp
R5441 T8518 T8516 punct ),bp
R5442 T8519 T8489 prep for,amplified
R5443 T8520 T8521 nummod 20,cycles
R5444 T8521 T8519 pobj cycles,for
R5445 T8522 T8520 punct ", ",20
R5446 T8523 T8520 conj 25,20
R5447 T8524 T8523 punct ", ",25
R5448 T8525 T8523 conj 30,25
R5449 T8526 T8525 cc or,30
R5450 T8527 T8525 conj 35,30
R5451 T8528 T8489 punct .,amplified
R5452 T8530 T8531 nsubjpass Atp5a1,included
R5453 T8532 T8533 punct (,bp
R5454 T8533 T8530 parataxis bp,Atp5a1
R5455 T8534 T8533 nummod 415,bp
R5456 T8535 T8533 punct ),bp
R5457 T8536 T8530 cc and,Atp5a1
R5458 T8537 T8530 conj Gapdh,Atp5a1
R5459 T8538 T8539 punct (,bp
R5460 T8539 T8537 parataxis bp,Gapdh
R5461 T8540 T8539 nummod 450,bp
R5462 T8541 T8539 punct ),bp
R5463 T8542 T8530 punct ", ",Atp5a1
R5464 T8543 T8544 dep which,expressed
R5465 T8544 T8530 relcl expressed,Atp5a1
R5466 T8545 T8544 auxpass are,expressed
R5467 T8546 T8544 advmod highly,expressed
R5468 T8547 T8544 prep in,expressed
R5469 T8548 T8549 det the,brain
R5470 T8549 T8547 pobj brain,in
R5471 T8550 T8531 punct ", ",included
R5472 T8551 T8531 auxpass are,included
R5473 T8552 T8531 prep for,included
R5474 T8553 T8552 pobj comparison,for
R5475 T8554 T8531 punct .,included
R5476 T8556 T8557 nsubj M,indicates
R5477 T8557 T8558 ccomp indicates,indicated
R5478 T8559 T8560 det the,marker
R5479 T8560 T8557 dobj marker,indicates
R5480 T8561 T8562 nmod Φ×,HaeIII
R5481 T8562 T8560 compound HaeIII,marker
R5482 T8563 T8562 nummod 174,HaeIII
R5483 T8564 T8565 compound DNA,size
R5484 T8565 T8560 compound size,marker
R5485 T8566 T8558 punct ;,indicated
R5486 T8567 T8568 det the,positions
R5487 T8568 T8558 nsubjpass positions,indicated
R5488 T8569 T8568 prep of,positions
R5489 T8570 T8571 det the,bands
R5490 T8571 T8569 pobj bands,of
R5491 T8572 T8573 nummod 603,bp
R5492 T8573 T8571 nmod bp,bands
R5493 T8574 T8573 cc and,bp
R5494 T8575 T8576 nummod 310,bp
R5495 T8576 T8573 conj bp,bp
R5496 T8577 T8571 compound marker,bands
R5497 T8578 T8558 auxpass are,indicated
R5498 T8579 T8558 punct .,indicated