PMC:1208879 JSON TXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T175	0-1	DT	denotes	A
T177	0-117	sentence	denotes	A screen for proteins that interact with PAX6: C-terminal mutations disrupt interaction with HOMER3, DNCL1 and TRIM11
T176	2-8	NN	denotes	screen
T179	9-12	IN	denotes	for
T180	13-21	NN	denotes	proteins
T181	22-26	WDT	denotes	that
T182	27-35	VBP	denotes	interact
T183	36-40	IN	denotes	with
T184	41-45	NN	denotes	PAX6
T185	45-47	:	denotes	:
T186	47-48	NN	denotes	C
T188	48-49	HYPH	denotes	-
T187	49-57	JJ	denotes	terminal
T189	58-67	NNS	denotes	mutations
T178	68-75	VBP	denotes	disrupt
T190	76-87	NN	denotes	interaction
T191	88-92	IN	denotes	with
T192	93-99	NN	denotes	HOMER3
T193	99-101	,	denotes	,
T194	101-106	NN	denotes	DNCL1
T195	107-110	CC	denotes	and
T196	111-117	NN	denotes	TRIM11
T197	117-118	sentence	denotes
T198	128-138	sentence	denotes	Background
T199	128-138	NN	denotes	Background
T200	138-242	sentence	denotes	The PAX6 protein is a transcriptional regulator with a key role in ocular and neurological development.
T201	139-142	DT	denotes	The
T203	143-147	NN	denotes	PAX6
T202	148-155	NN	denotes	protein
T204	156-158	VBZ	denotes	is
T205	159-160	DT	denotes	a
T207	161-176	JJ	denotes	transcriptional
T206	177-186	NN	denotes	regulator
T208	187-191	IN	denotes	with
T209	192-193	DT	denotes	a
T211	194-197	JJ	denotes	key
T210	198-202	NN	denotes	role
T212	203-205	IN	denotes	in
T213	206-212	JJ	denotes	ocular
T215	213-216	CC	denotes	and
T216	217-229	JJ	denotes	neurological
T214	230-241	NN	denotes	development
T217	241-242	.	denotes	.
T218	242-357	sentence	denotes	Individuals with heterozygous loss-of-function mutations in the PAX6 gene have malformations of the eye and brain.
T219	243-254	NNS	denotes	Individuals
T221	255-259	IN	denotes	with
T222	260-272	JJ	denotes	heterozygous
T224	273-277	NN	denotes	loss
T225	277-278	HYPH	denotes	-
T226	278-280	IN	denotes	of
T227	280-281	HYPH	denotes	-
T228	281-289	NN	denotes	function
T223	290-299	NNS	denotes	mutations
T229	300-302	IN	denotes	in
T230	303-306	DT	denotes	the
T232	307-311	NN	denotes	PAX6
T231	312-316	NN	denotes	gene
T220	317-321	VBP	denotes	have
T233	322-335	NNS	denotes	malformations
T234	336-338	IN	denotes	of
T235	339-342	DT	denotes	the
T236	343-346	NN	denotes	eye
T237	347-350	CC	denotes	and
T238	351-356	NN	denotes	brain
T239	356-357	.	denotes	.
T240	357-501	sentence	denotes	Little is known about the interactions of PAX6 with other proteins, so we carried out a systematic screen for proteins that interact with PAX6.
T241	358-364	NN	denotes	Little
T243	365-367	VBZ	denotes	is
T242	368-373	VBN	denotes	known
T244	374-379	IN	denotes	about
T245	380-383	DT	denotes	the
T246	384-396	NNS	denotes	interactions
T247	397-399	IN	denotes	of
T248	400-404	NN	denotes	PAX6
T249	405-409	IN	denotes	with
T250	410-415	JJ	denotes	other
T251	416-424	NN	denotes	proteins
T252	424-426	,	denotes	,
T253	426-428	CC	denotes	so
T254	429-431	PRP	denotes	we
T255	432-439	VBD	denotes	carried
T256	440-443	RP	denotes	out
T257	444-445	DT	denotes	a
T259	446-456	JJ	denotes	systematic
T258	457-463	NN	denotes	screen
T260	464-467	IN	denotes	for
T261	468-476	NN	denotes	proteins
T262	477-481	WDT	denotes	that
T263	482-490	VBP	denotes	interact
T264	491-495	IN	denotes	with
T265	496-500	NN	denotes	PAX6
T266	500-501	.	denotes	.
T267	501-510	sentence	denotes	Results
T268	503-510	NNS	denotes	Results
T269	510-626	sentence	denotes	We used bioinformatics techniques to characterise a highly conserved peptide at the C-terminus of the PAX6 protein.
T270	511-513	PRP	denotes	We
T271	514-518	VBD	denotes	used
T272	519-533	NN	denotes	bioinformatics
T273	534-544	NNS	denotes	techniques
T274	545-547	TO	denotes	to
T275	548-560	VB	denotes	characterise
T276	561-562	DT	denotes	a
T278	563-569	RB	denotes	highly
T279	570-579	VBN	denotes	conserved
T277	580-587	NN	denotes	peptide
T280	588-590	IN	denotes	at
T281	591-594	DT	denotes	the
T283	595-596	NN	denotes	C
T284	596-597	HYPH	denotes	-
T282	597-605	NN	denotes	terminus
T285	606-608	IN	denotes	of
T286	609-612	DT	denotes	the
T288	613-617	NN	denotes	PAX6
T287	618-625	NN	denotes	protein
T289	625-626	.	denotes	.
T290	626-823	sentence	denotes	Yeast two-hybrid library screens were then carried out to identify brain-expressed proteins that interact with the C-terminal peptide and with the entire PAX6 proline-serine-threonine-rich domain.
T291	627-632	NN	denotes	Yeast
T293	633-636	CD	denotes	two
T295	636-637	HYPH	denotes	-
T294	637-643	NN	denotes	hybrid
T296	644-651	NN	denotes	library
T292	652-659	NNS	denotes	screens
T298	660-664	VBD	denotes	were
T299	665-669	RB	denotes	then
T297	670-677	VBN	denotes	carried
T300	678-681	RP	denotes	out
T301	682-684	TO	denotes	to
T302	685-693	VB	denotes	identify
T303	694-699	NN	denotes	brain
T305	699-700	HYPH	denotes	-
T304	700-709	VBN	denotes	expressed
T306	710-718	NN	denotes	proteins
T307	719-723	WDT	denotes	that
T308	724-732	VBP	denotes	interact
T309	733-737	IN	denotes	with
T310	738-741	DT	denotes	the
T312	742-743	NN	denotes	C
T314	743-744	HYPH	denotes	-
T313	744-752	JJ	denotes	terminal
T311	753-760	NN	denotes	peptide
T315	761-764	CC	denotes	and
T316	765-769	IN	denotes	with
T317	770-773	DT	denotes	the
T319	774-780	JJ	denotes	entire
T320	781-785	NN	denotes	PAX6
T321	786-793	NN	denotes	proline
T323	793-794	HYPH	denotes	-
T324	794-800	NN	denotes	serine
T325	800-801	HYPH	denotes	-
T322	801-810	NN	denotes	threonine
T327	810-811	HYPH	denotes	-
T326	811-815	JJ	denotes	rich
T318	816-822	NN	denotes	domain
T328	822-823	.	denotes	.
T329	823-993	sentence	denotes	Three novel PAX6-interacting proteins were identified: the post-synaptic density (PSD) protein HOMER3, the dynein subunit DNCL1, and the tripartite motif protein TRIM11.
T330	824-829	CD	denotes	Three
T332	830-835	JJ	denotes	novel
T333	836-840	NN	denotes	PAX6
T335	840-841	HYPH	denotes	-
T334	841-852	VBG	denotes	interacting
T331	853-861	NN	denotes	proteins
T337	862-866	VBD	denotes	were
T336	867-877	VBN	denotes	identified
T338	877-879	:	denotes	:
T339	879-882	DT	denotes	the
T341	883-896	JJ	denotes	post-synaptic
T342	897-904	NN	denotes	density
T343	905-906	-LRB-	denotes	(
T344	906-909	NN	denotes	PSD
T345	909-910	-RRB-	denotes	)
T340	911-918	NN	denotes	protein
T346	919-925	NN	denotes	HOMER3
T347	925-927	,	denotes	,
T348	927-930	DT	denotes	the
T350	931-937	NN	denotes	dynein
T349	938-945	NN	denotes	subunit
T351	946-951	NN	denotes	DNCL1
T352	951-953	,	denotes	,
T353	953-956	CC	denotes	and
T354	957-960	DT	denotes	the
T356	961-971	JJ	denotes	tripartite
T357	972-977	NN	denotes	motif
T355	978-985	NN	denotes	protein
T358	986-992	NN	denotes	TRIM11
T359	992-993	.	denotes	.
T360	993-1127	sentence	denotes	Three C-terminal PAX6 mutations, previously identified in patients with eye malformations, all reduced or abolished the interactions.
T361	994-999	CD	denotes	Three
T363	1000-1001	NN	denotes	C
T365	1001-1002	HYPH	denotes	-
T364	1002-1010	JJ	denotes	terminal
T366	1011-1015	NN	denotes	PAX6
T362	1016-1025	NNS	denotes	mutations
T368	1025-1027	,	denotes	,
T369	1027-1037	RB	denotes	previously
T370	1038-1048	VBN	denotes	identified
T371	1049-1051	IN	denotes	in
T372	1052-1060	NNS	denotes	patients
T373	1061-1065	IN	denotes	with
T374	1066-1069	NN	denotes	eye
T375	1070-1083	NNS	denotes	malformations
T376	1083-1085	,	denotes	,
T377	1085-1088	DT	denotes	all
T367	1089-1096	VBD	denotes	reduced
T378	1097-1099	CC	denotes	or
T379	1100-1109	VBD	denotes	abolished
T380	1110-1113	DT	denotes	the
T381	1114-1126	NNS	denotes	interactions
T382	1126-1127	.	denotes	.
T383	1127-1139	sentence	denotes	Conclusion
T384	1129-1139	NN	denotes	Conclusion
T385	1139-1219	sentence	denotes	Our preliminary data suggest that PAX6 interacts with HOMER3, DNCL1 and TRIM11.
T386	1140-1143	PRP$	denotes	Our
T388	1144-1155	JJ	denotes	preliminary
T387	1156-1160	NNS	denotes	data
T389	1161-1168	VBP	denotes	suggest
T390	1169-1173	IN	denotes	that
T392	1174-1178	NN	denotes	PAX6
T391	1179-1188	VBZ	denotes	interacts
T393	1189-1193	IN	denotes	with
T394	1194-1200	NN	denotes	HOMER3
T395	1200-1202	,	denotes	,
T396	1202-1207	NN	denotes	DNCL1
T397	1208-1211	CC	denotes	and
T398	1212-1218	NN	denotes	TRIM11
T399	1218-1219	.	denotes	.
T400	1219-1516	sentence	denotes	We propose that the interaction of PAX6 with HOMER3 and DNCL1 is a mechanism by which synaptic activation could lead to changes in neuronal transcriptional activity, and that some of the neural anomalies in patients with PAX6 mutations could be explained by impaired protein-protein interactions.
T401	1220-1222	PRP	denotes	We
T402	1223-1230	VBP	denotes	propose
T403	1231-1235	IN	denotes	that
T405	1236-1239	DT	denotes	the
T406	1240-1251	NN	denotes	interaction
T407	1252-1254	IN	denotes	of
T408	1255-1259	NN	denotes	PAX6
T409	1260-1264	IN	denotes	with
T410	1265-1271	NN	denotes	HOMER3
T411	1272-1275	CC	denotes	and
T412	1276-1281	NN	denotes	DNCL1
T404	1282-1284	VBZ	denotes	is
T413	1285-1286	DT	denotes	a
T414	1287-1296	NN	denotes	mechanism
T415	1297-1299	IN	denotes	by
T417	1300-1305	WDT	denotes	which
T418	1306-1314	JJ	denotes	synaptic
T419	1315-1325	NN	denotes	activation
T420	1326-1331	MD	denotes	could
T416	1332-1336	VB	denotes	lead
T421	1337-1339	IN	denotes	to
T422	1340-1347	NNS	denotes	changes
T423	1348-1350	IN	denotes	in
T424	1351-1359	JJ	denotes	neuronal
T426	1360-1375	JJ	denotes	transcriptional
T425	1376-1384	NN	denotes	activity
T427	1384-1386	,	denotes	,
T428	1386-1389	CC	denotes	and
T429	1390-1394	IN	denotes	that
T431	1395-1399	DT	denotes	some
T432	1400-1402	IN	denotes	of
T433	1403-1406	DT	denotes	the
T435	1407-1413	JJ	denotes	neural
T434	1414-1423	NNS	denotes	anomalies
T436	1424-1426	IN	denotes	in
T437	1427-1435	NNS	denotes	patients
T438	1436-1440	IN	denotes	with
T439	1441-1445	NN	denotes	PAX6
T440	1446-1455	NNS	denotes	mutations
T441	1456-1461	MD	denotes	could
T442	1462-1464	VB	denotes	be
T430	1465-1474	VBN	denotes	explained
T443	1475-1477	IN	denotes	by
T444	1478-1486	JJ	denotes	impaired
T446	1487-1494	NN	denotes	protein
T448	1494-1495	SYM	denotes	-
T447	1495-1502	NN	denotes	protein
T445	1503-1515	NNS	denotes	interactions
T449	1515-1516	.	denotes	.
T834	1529-1532	DT	denotes	The
T836	1533-1537	NN	denotes	PAX6
T835	1538-1545	NN	denotes	protein
T837	1546-1548	VBZ	denotes	is
T838	1549-1550	DT	denotes	a
T839	1551-1557	NN	denotes	member
T840	1558-1560	IN	denotes	of
T841	1561-1564	DT	denotes	the
T843	1565-1568	NN	denotes	PAX
T844	1569-1570	-LRB-	denotes	(
T845	1570-1576	VBN	denotes	paired
T847	1576-1577	HYPH	denotes	-
T846	1577-1580	NN	denotes	box
T848	1580-1581	-RRB-	denotes	)
T842	1582-1588	NN	denotes	family
T849	1589-1591	IN	denotes	of
T850	1592-1607	JJ	denotes	transcriptional
T851	1608-1618	NNS	denotes	regulators
T852	1619-1622	CC	denotes	and
T853	1623-1625	VBZ	denotes	is
T854	1626-1635	JJ	denotes	essential
T855	1636-1639	IN	denotes	for
T856	1640-1646	JJ	denotes	normal
T858	1647-1653	JJ	denotes	ocular
T859	1654-1657	CC	denotes	and
T860	1658-1664	JJ	denotes	neural
T857	1665-1676	NN	denotes	development
T861	1677-1678	-LRB-	denotes	[
T862	1678-1679	CD	denotes	1
T863	1679-1680	-RRB-	denotes	]
T864	1680-1681	.	denotes	.
T865	1681-1819	sentence	denotes	Heterozygous mutations of the human PAX6 gene cause aniridia (absence of the iris) and a range of other congenital eye malformations [2].
T866	1682-1694	JJ	denotes	Heterozygous
T867	1695-1704	NNS	denotes	mutations
T869	1705-1707	IN	denotes	of
T870	1708-1711	DT	denotes	the
T872	1712-1717	JJ	denotes	human
T873	1718-1722	NN	denotes	PAX6
T871	1723-1727	NN	denotes	gene
T868	1728-1733	VBP	denotes	cause
T874	1734-1742	NN	denotes	aniridia
T875	1743-1744	-LRB-	denotes	(
T876	1744-1751	NN	denotes	absence
T877	1752-1754	IN	denotes	of
T878	1755-1758	DT	denotes	the
T879	1759-1763	NN	denotes	iris
T880	1763-1764	-RRB-	denotes	)
T881	1765-1768	CC	denotes	and
T882	1769-1770	DT	denotes	a
T883	1771-1776	NN	denotes	range
T884	1777-1779	IN	denotes	of
T885	1780-1785	JJ	denotes	other
T887	1786-1796	JJ	denotes	congenital
T888	1797-1800	NN	denotes	eye
T886	1801-1814	NNS	denotes	malformations
T889	1815-1816	-LRB-	denotes	[
T890	1816-1817	CD	denotes	2
T891	1817-1818	-RRB-	denotes	]
T892	1818-1819	.	denotes	.
T893	1819-1936	sentence	denotes	Neural defects such as foveal hypoplasia and optic nerve hypoplasia are common in PAX6-associated eye disease [3-5].
T894	1820-1826	JJ	denotes	Neural
T895	1827-1834	NNS	denotes	defects
T897	1835-1839	JJ	denotes	such
T898	1840-1842	IN	denotes	as
T899	1843-1849	JJ	denotes	foveal
T900	1850-1860	NN	denotes	hypoplasia
T901	1861-1864	CC	denotes	and
T902	1865-1870	JJ	denotes	optic
T903	1871-1876	NN	denotes	nerve
T904	1877-1887	NN	denotes	hypoplasia
T896	1888-1891	VBP	denotes	are
T905	1892-1898	JJ	denotes	common
T906	1899-1901	IN	denotes	in
T907	1902-1906	NN	denotes	PAX6
T909	1906-1907	HYPH	denotes	-
T908	1907-1917	VBN	denotes	associated
T911	1918-1921	NN	denotes	eye
T910	1922-1929	NN	denotes	disease
T912	1930-1931	-LRB-	denotes	[
T913	1931-1932	CD	denotes	3
T914	1932-1933	SYM	denotes	-
T915	1933-1934	CD	denotes	5
T916	1934-1935	-RRB-	denotes	]
T917	1935-1936	.	denotes	.
T918	1936-2140	sentence	denotes	Homozygous mutations in man and mouse are lethal and result in severe developmental abnormalities including anophthalmia, severe reduction of the olfactory structures and gross brain malformations [2,6].
T919	1937-1947	JJ	denotes	Homozygous
T920	1948-1957	NNS	denotes	mutations
T922	1958-1960	IN	denotes	in
T923	1961-1964	NN	denotes	man
T924	1965-1968	CC	denotes	and
T925	1969-1974	NN	denotes	mouse
T921	1975-1978	VBP	denotes	are
T926	1979-1985	JJ	denotes	lethal
T927	1986-1989	CC	denotes	and
T928	1990-1996	VBP	denotes	result
T929	1997-1999	IN	denotes	in
T930	2000-2006	JJ	denotes	severe
T932	2007-2020	JJ	denotes	developmental
T931	2021-2034	NNS	denotes	abnormalities
T933	2035-2044	VBG	denotes	including
T934	2045-2057	NN	denotes	anophthalmia
T935	2057-2059	,	denotes	,
T936	2059-2065	JJ	denotes	severe
T937	2066-2075	NN	denotes	reduction
T938	2076-2078	IN	denotes	of
T939	2079-2082	DT	denotes	the
T941	2083-2092	JJ	denotes	olfactory
T940	2093-2103	NNS	denotes	structures
T942	2104-2107	CC	denotes	and
T943	2108-2113	JJ	denotes	gross
T945	2114-2119	NN	denotes	brain
T944	2120-2133	NNS	denotes	malformations
T946	2134-2135	-LRB-	denotes	[
T948	2135-2136	CD	denotes	2
T949	2136-2137	,	denotes	,
T947	2137-2138	CD	denotes	6
T950	2138-2139	-RRB-	denotes	]
T951	2139-2140	.	denotes	.
T952	2140-2458	sentence	denotes	The roles of PAX6 in brain development have mainly been studied in homozygous mutant mice or rats and include arealisation of the cerebral cortex [7], formation of the prosencephalon-mesencephalon boundary [8], axon guidance [8], differentiation of neurons from glia [9] and neuronal migration in the cerebellum [10].
T953	2141-2144	DT	denotes	The
T954	2145-2150	NNS	denotes	roles
T956	2151-2153	IN	denotes	of
T957	2154-2158	NN	denotes	PAX6
T958	2159-2161	IN	denotes	in
T959	2162-2167	NN	denotes	brain
T960	2168-2179	NN	denotes	development
T961	2180-2184	VBP	denotes	have
T962	2185-2191	RB	denotes	mainly
T963	2192-2196	VBN	denotes	been
T955	2197-2204	VBN	denotes	studied
T964	2205-2207	IN	denotes	in
T965	2208-2218	JJ	denotes	homozygous
T967	2219-2225	NN	denotes	mutant
T966	2226-2230	NNS	denotes	mice
T968	2231-2233	CC	denotes	or
T969	2234-2238	NNS	denotes	rats
T970	2239-2242	CC	denotes	and
T971	2243-2250	VBP	denotes	include
T972	2251-2263	NN	denotes	arealisation
T973	2264-2266	IN	denotes	of
T974	2267-2270	DT	denotes	the
T976	2271-2279	JJ	denotes	cerebral
T975	2280-2286	NN	denotes	cortex
T977	2287-2288	-LRB-	denotes	[
T978	2288-2289	CD	denotes	7
T979	2289-2290	-RRB-	denotes	]
T980	2290-2292	,	denotes	,
T981	2292-2301	NN	denotes	formation
T982	2302-2304	IN	denotes	of
T983	2305-2308	DT	denotes	the
T985	2309-2323	NN	denotes	prosencephalon
T987	2323-2324	HYPH	denotes	-
T986	2324-2337	NN	denotes	mesencephalon
T984	2338-2346	NN	denotes	boundary
T988	2347-2348	-LRB-	denotes	[
T989	2348-2349	CD	denotes	8
T990	2349-2350	-RRB-	denotes	]
T991	2350-2352	,	denotes	,
T992	2352-2356	NN	denotes	axon
T993	2357-2365	NN	denotes	guidance
T994	2366-2367	-LRB-	denotes	[
T995	2367-2368	CD	denotes	8
T996	2368-2369	-RRB-	denotes	]
T997	2369-2371	,	denotes	,
T998	2371-2386	NN	denotes	differentiation
T999	2387-2389	IN	denotes	of
T1000	2390-2397	NNS	denotes	neurons
T1001	2398-2402	IN	denotes	from
T1002	2403-2407	NNS	denotes	glia
T1003	2408-2409	-LRB-	denotes	[
T1004	2409-2410	CD	denotes	9
T1005	2410-2411	-RRB-	denotes	]
T1006	2412-2415	CC	denotes	and
T1007	2416-2424	JJ	denotes	neuronal
T1008	2425-2434	NN	denotes	migration
T1009	2435-2437	IN	denotes	in
T1010	2438-2441	DT	denotes	the
T1011	2442-2452	NN	denotes	cerebellum
T1012	2453-2454	-LRB-	denotes	[
T1013	2454-2456	CD	denotes	10
T1014	2456-2457	-RRB-	denotes	]
T1015	2457-2458	.	denotes	.
T1016	2458-2635	sentence	denotes	The discovery of multiple and diverse roles for PAX6 in brain development prompted MRI analyses of aniridia patients, and a range of distinctive brain anomalies were uncovered.
T1017	2459-2462	DT	denotes	The
T1018	2463-2472	NN	denotes	discovery
T1020	2473-2475	IN	denotes	of
T1021	2476-2484	JJ	denotes	multiple
T1023	2485-2488	CC	denotes	and
T1024	2489-2496	JJ	denotes	diverse
T1022	2497-2502	NNS	denotes	roles
T1025	2503-2506	IN	denotes	for
T1026	2507-2511	NN	denotes	PAX6
T1027	2512-2514	IN	denotes	in
T1028	2515-2520	NN	denotes	brain
T1029	2521-2532	NN	denotes	development
T1019	2533-2541	VBD	denotes	prompted
T1030	2542-2545	NN	denotes	MRI
T1031	2546-2554	NNS	denotes	analyses
T1032	2555-2557	IN	denotes	of
T1033	2558-2566	NN	denotes	aniridia
T1034	2567-2575	NNS	denotes	patients
T1035	2575-2577	,	denotes	,
T1036	2577-2580	CC	denotes	and
T1037	2581-2582	DT	denotes	a
T1038	2583-2588	NN	denotes	range
T1040	2589-2591	IN	denotes	of
T1041	2592-2603	JJ	denotes	distinctive
T1043	2604-2609	NN	denotes	brain
T1042	2610-2619	NNS	denotes	anomalies
T1044	2620-2624	VBD	denotes	were
T1039	2625-2634	VBN	denotes	uncovered
T1045	2634-2635	.	denotes	.
T1046	2635-2732	sentence	denotes	The most common and striking of these was absence or hypoplasia of the anterior commissure [11].
T1047	2636-2639	DT	denotes	The
T1049	2640-2644	RBS	denotes	most
T1048	2645-2651	JJ	denotes	common
T1051	2652-2655	CC	denotes	and
T1052	2656-2664	JJ	denotes	striking
T1053	2665-2667	IN	denotes	of
T1054	2668-2673	DT	denotes	these
T1050	2674-2677	VBD	denotes	was
T1055	2678-2685	NN	denotes	absence
T1056	2686-2688	CC	denotes	or
T1057	2689-2699	NN	denotes	hypoplasia
T1058	2700-2702	IN	denotes	of
T1059	2703-2706	DT	denotes	the
T1061	2707-2715	JJ	denotes	anterior
T1060	2716-2726	NN	denotes	commissure
T1062	2727-2728	-LRB-	denotes	[
T1063	2728-2730	CD	denotes	11
T1064	2730-2731	-RRB-	denotes	]
T1065	2731-2732	.	denotes	.
T1066	2732-2918	sentence	denotes	Other defects included absence or hypoplasia of the pineal gland, cortical polymicrogyria, white matter changes in the corpus callosum and grey matter changes in the cerebellum [11-13].
T1067	2733-2738	JJ	denotes	Other
T1068	2739-2746	NNS	denotes	defects
T1069	2747-2755	VBD	denotes	included
T1070	2756-2763	NN	denotes	absence
T1071	2764-2766	CC	denotes	or
T1072	2767-2777	NN	denotes	hypoplasia
T1073	2778-2780	IN	denotes	of
T1074	2781-2784	DT	denotes	the
T1076	2785-2791	JJ	denotes	pineal
T1075	2792-2797	NN	denotes	gland
T1077	2797-2799	,	denotes	,
T1078	2799-2807	JJ	denotes	cortical
T1079	2808-2822	NN	denotes	polymicrogyria
T1080	2822-2824	,	denotes	,
T1081	2824-2829	JJ	denotes	white
T1082	2830-2836	NN	denotes	matter
T1083	2837-2844	NNS	denotes	changes
T1084	2845-2847	IN	denotes	in
T1085	2848-2851	DT	denotes	the
T1087	2852-2858	NN	denotes	corpus
T1086	2859-2867	NN	denotes	callosum
T1088	2868-2871	CC	denotes	and
T1089	2872-2876	JJ	denotes	grey
T1090	2877-2883	NN	denotes	matter
T1091	2884-2891	NNS	denotes	changes
T1092	2892-2894	IN	denotes	in
T1093	2895-2898	DT	denotes	the
T1094	2899-2909	NN	denotes	cerebellum
T1095	2910-2911	-LRB-	denotes	[
T1096	2911-2913	CD	denotes	11
T1097	2913-2914	SYM	denotes	-
T1098	2914-2916	CD	denotes	13
T1099	2916-2917	-RRB-	denotes	]
T1100	2917-2918	.	denotes	.
T1101	2918-3013	sentence	denotes	Functional changes included hyposmia and abnormal inter-hemispheric auditory transfer [11,14].
T1102	2919-2929	JJ	denotes	Functional
T1103	2930-2937	NNS	denotes	changes
T1104	2938-2946	VBD	denotes	included
T1105	2947-2955	NN	denotes	hyposmia
T1106	2956-2959	CC	denotes	and
T1107	2960-2968	JJ	denotes	abnormal
T1109	2969-2986	JJ	denotes	inter-hemispheric
T1110	2987-2995	JJ	denotes	auditory
T1108	2996-3004	NN	denotes	transfer
T1111	3005-3006	-LRB-	denotes	[
T1113	3006-3008	CD	denotes	11
T1114	3008-3009	,	denotes	,
T1112	3009-3011	CD	denotes	14
T1115	3011-3012	-RRB-	denotes	]
T1116	3012-3013	.	denotes	.
T1117	3013-3167	sentence	denotes	The defining feature of all PAX proteins is the presence of a 128 amino acid DNA-binding paired domain that has been highly conserved over evolution [1].
T1118	3014-3017	DT	denotes	The
T1120	3018-3026	VBG	denotes	defining
T1119	3027-3034	NN	denotes	feature
T1122	3035-3037	IN	denotes	of
T1123	3038-3041	DT	denotes	all
T1125	3042-3045	NN	denotes	PAX
T1124	3046-3054	NN	denotes	proteins
T1121	3055-3057	VBZ	denotes	is
T1126	3058-3061	DT	denotes	the
T1127	3062-3070	NN	denotes	presence
T1128	3071-3073	IN	denotes	of
T1129	3074-3075	DT	denotes	a
T1131	3076-3079	CD	denotes	128
T1133	3080-3085	NN	denotes	amino
T1132	3086-3090	NN	denotes	acid
T1134	3091-3094	NN	denotes	DNA
T1136	3094-3095	HYPH	denotes	-
T1135	3095-3102	VBG	denotes	binding
T1137	3103-3109	JJ	denotes	paired
T1130	3110-3116	NN	denotes	domain
T1138	3117-3121	WDT	denotes	that
T1140	3122-3125	VBZ	denotes	has
T1141	3126-3130	VBN	denotes	been
T1142	3131-3137	RB	denotes	highly
T1139	3138-3147	VBN	denotes	conserved
T1143	3148-3152	IN	denotes	over
T1144	3153-3162	NN	denotes	evolution
T1145	3163-3164	-LRB-	denotes	[
T1146	3164-3165	CD	denotes	1
T1147	3165-3166	-RRB-	denotes	]
T1148	3166-3167	.	denotes	.
T1149	3167-3325	sentence	denotes	In addition to the paired domain, PAX6 also contains a DNA-binding homeodomain and a proline, serine and threonine-rich (PST) domain at the C-terminus [1,6].
T1150	3168-3170	IN	denotes	In
T1152	3171-3179	NN	denotes	addition
T1153	3180-3182	IN	denotes	to
T1154	3183-3186	DT	denotes	the
T1156	3187-3193	JJ	denotes	paired
T1155	3194-3200	NN	denotes	domain
T1157	3200-3202	,	denotes	,
T1158	3202-3206	NN	denotes	PAX6
T1159	3207-3211	RB	denotes	also
T1151	3212-3220	VBZ	denotes	contains
T1160	3221-3222	DT	denotes	a
T1162	3223-3226	NN	denotes	DNA
T1164	3226-3227	HYPH	denotes	-
T1163	3227-3234	VBG	denotes	binding
T1161	3235-3246	NN	denotes	homeodomain
T1165	3247-3250	CC	denotes	and
T1166	3251-3252	DT	denotes	a
T1168	3253-3260	NN	denotes	proline
T1170	3260-3262	,	denotes	,
T1171	3262-3268	NN	denotes	serine
T1172	3269-3272	CC	denotes	and
T1173	3273-3282	NN	denotes	threonine
T1174	3282-3283	HYPH	denotes	-
T1169	3283-3287	JJ	denotes	rich
T1175	3288-3289	-LRB-	denotes	(
T1176	3289-3292	NN	denotes	PST
T1177	3292-3293	-RRB-	denotes	)
T1167	3294-3300	NN	denotes	domain
T1178	3301-3303	IN	denotes	at
T1179	3304-3307	DT	denotes	the
T1181	3308-3309	NN	denotes	C
T1182	3309-3310	HYPH	denotes	-
T1180	3310-3318	NN	denotes	terminus
T1183	3319-3320	-LRB-	denotes	[
T1185	3320-3321	CD	denotes	1
T1186	3321-3322	,	denotes	,
T1184	3322-3323	CD	denotes	6
T1187	3323-3324	-RRB-	denotes	]
T1188	3324-3325	.	denotes	.
T1189	3325-3457	sentence	denotes	The PST domain, which encompasses the C-terminal 145 amino acids of PAX6, has been shown to act as a transcriptional activator [6].
T1190	3326-3329	DT	denotes	The
T1192	3330-3333	NN	denotes	PST
T1191	3334-3340	NN	denotes	domain
T1194	3340-3342	,	denotes	,
T1195	3342-3347	WDT	denotes	which
T1196	3348-3359	VBZ	denotes	encompasses
T1197	3360-3363	DT	denotes	the
T1199	3364-3365	NN	denotes	C
T1201	3365-3366	HYPH	denotes	-
T1200	3366-3374	JJ	denotes	terminal
T1202	3375-3378	CD	denotes	145
T1203	3379-3384	NN	denotes	amino
T1198	3385-3390	NNS	denotes	acids
T1204	3391-3393	IN	denotes	of
T1205	3394-3398	NN	denotes	PAX6
T1206	3398-3400	,	denotes	,
T1207	3400-3403	VBZ	denotes	has
T1208	3404-3408	VBN	denotes	been
T1193	3409-3414	VBN	denotes	shown
T1209	3415-3417	TO	denotes	to
T1210	3418-3421	VB	denotes	act
T1211	3422-3424	IN	denotes	as
T1212	3425-3426	DT	denotes	a
T1214	3427-3442	JJ	denotes	transcriptional
T1213	3443-3452	NN	denotes	activator
T1215	3453-3454	-LRB-	denotes	[
T1216	3454-3455	CD	denotes	6
T1217	3455-3456	-RRB-	denotes	]
T1218	3456-3457	.	denotes	.
T1219	3457-3578	sentence	denotes	The PAX6 protein directly regulates a wide range of target genes [1,2] including Pax2 [15], Ngn2 [16] and glucagon [17].
T1220	3458-3461	DT	denotes	The
T1222	3462-3466	NN	denotes	PAX6
T1221	3467-3474	NN	denotes	protein
T1224	3475-3483	RB	denotes	directly
T1223	3484-3493	VBZ	denotes	regulates
T1225	3494-3495	DT	denotes	a
T1227	3496-3500	JJ	denotes	wide
T1226	3501-3506	NN	denotes	range
T1228	3507-3509	IN	denotes	of
T1229	3510-3516	NN	denotes	target
T1230	3517-3522	NNS	denotes	genes
T1231	3523-3524	-LRB-	denotes	[
T1233	3524-3525	CD	denotes	1
T1234	3525-3526	,	denotes	,
T1232	3526-3527	CD	denotes	2
T1235	3527-3528	-RRB-	denotes	]
T1236	3529-3538	VBG	denotes	including
T1237	3539-3543	NN	denotes	Pax2
T1238	3544-3545	-LRB-	denotes	[
T1239	3545-3547	CD	denotes	15
T1240	3547-3548	-RRB-	denotes	]
T1241	3548-3550	,	denotes	,
T1242	3550-3554	NN	denotes	Ngn2
T1243	3555-3556	-LRB-	denotes	[
T1244	3556-3558	CD	denotes	16
T1245	3558-3559	-RRB-	denotes	]
T1246	3560-3563	CC	denotes	and
T1247	3564-3572	NN	denotes	glucagon
T1248	3573-3574	-LRB-	denotes	[
T1249	3574-3576	CD	denotes	17
T1250	3576-3577	-RRB-	denotes	]
T1251	3577-3578	.	denotes	.
T1252	3578-3717	sentence	denotes	The Pax6 gene has a spatially and temporally complex expression pattern in the eye, brain, nasal structures, spinal cord and pancreas [1].
T1253	3579-3582	DT	denotes	The
T1255	3583-3587	NN	denotes	Pax6
T1254	3588-3592	NN	denotes	gene
T1256	3593-3596	VBZ	denotes	has
T1257	3597-3598	DT	denotes	a
T1259	3599-3608	RB	denotes	spatially
T1261	3609-3612	CC	denotes	and
T1262	3613-3623	RB	denotes	temporally
T1260	3624-3631	JJ	denotes	complex
T1263	3632-3642	NN	denotes	expression
T1258	3643-3650	NN	denotes	pattern
T1264	3651-3653	IN	denotes	in
T1265	3654-3657	DT	denotes	the
T1266	3658-3661	NN	denotes	eye
T1267	3661-3663	,	denotes	,
T1268	3663-3668	NN	denotes	brain
T1269	3668-3670	,	denotes	,
T1270	3670-3675	JJ	denotes	nasal
T1271	3676-3686	NNS	denotes	structures
T1272	3686-3688	,	denotes	,
T1273	3688-3694	JJ	denotes	spinal
T1274	3695-3699	NN	denotes	cord
T1275	3700-3703	CC	denotes	and
T1276	3704-3712	NN	denotes	pancreas
T1277	3713-3714	-LRB-	denotes	[
T1278	3714-3715	CD	denotes	1
T1279	3715-3716	-RRB-	denotes	]
T1280	3716-3717	.	denotes	.
T1281	3717-3866	sentence	denotes	Although PAX6 is clearly involved in multiple developmental processes, common themes are now emerging concerning the role of PAX6 in neural tissues.
T1282	3718-3726	IN	denotes	Although
T1284	3727-3731	NN	denotes	PAX6
T1285	3732-3734	VBZ	denotes	is
T1286	3735-3742	RB	denotes	clearly
T1283	3743-3751	VBN	denotes	involved
T1288	3752-3754	IN	denotes	in
T1289	3755-3763	JJ	denotes	multiple
T1291	3764-3777	JJ	denotes	developmental
T1290	3778-3787	NNS	denotes	processes
T1292	3787-3789	,	denotes	,
T1293	3789-3795	JJ	denotes	common
T1294	3796-3802	NNS	denotes	themes
T1295	3803-3806	VBP	denotes	are
T1296	3807-3810	RB	denotes	now
T1287	3811-3819	VBG	denotes	emerging
T1297	3820-3830	VBG	denotes	concerning
T1298	3831-3834	DT	denotes	the
T1299	3835-3839	NN	denotes	role
T1300	3840-3842	IN	denotes	of
T1301	3843-3847	NN	denotes	PAX6
T1302	3848-3850	IN	denotes	in
T1303	3851-3857	JJ	denotes	neural
T1304	3858-3865	NNS	denotes	tissues
T1305	3865-3866	.	denotes	.
T1306	3866-3994	sentence	denotes	Gradients of Pax6 expression are important for determining positional characteristics in the retina [18] and the neocortex [7].
T1307	3867-3876	NNS	denotes	Gradients
T1309	3877-3879	IN	denotes	of
T1310	3880-3884	NN	denotes	Pax6
T1311	3885-3895	NN	denotes	expression
T1308	3896-3899	VBP	denotes	are
T1312	3900-3909	JJ	denotes	important
T1313	3910-3913	IN	denotes	for
T1314	3914-3925	VBG	denotes	determining
T1315	3926-3936	JJ	denotes	positional
T1316	3937-3952	NNS	denotes	characteristics
T1317	3953-3955	IN	denotes	in
T1318	3956-3959	DT	denotes	the
T1319	3960-3966	NN	denotes	retina
T1320	3967-3968	-LRB-	denotes	[
T1321	3968-3970	CD	denotes	18
T1322	3970-3971	-RRB-	denotes	]
T1323	3972-3975	CC	denotes	and
T1324	3976-3979	DT	denotes	the
T1325	3980-3989	NN	denotes	neocortex
T1326	3990-3991	-LRB-	denotes	[
T1327	3991-3992	CD	denotes	7
T1328	3992-3993	-RRB-	denotes	]
T1329	3993-3994	.	denotes	.
T1330	3994-4129	sentence	denotes	PAX6 plays a role in development of specific axonal connections between the retina and the brain [18] and within the forebrain [8,19].
T1331	3995-3999	NN	denotes	PAX6
T1332	4000-4005	VBZ	denotes	plays
T1333	4006-4007	DT	denotes	a
T1334	4008-4012	NN	denotes	role
T1335	4013-4015	IN	denotes	in
T1336	4016-4027	NN	denotes	development
T1337	4028-4030	IN	denotes	of
T1338	4031-4039	JJ	denotes	specific
T1340	4040-4046	JJ	denotes	axonal
T1339	4047-4058	NNS	denotes	connections
T1341	4059-4066	IN	denotes	between
T1342	4067-4070	DT	denotes	the
T1343	4071-4077	NN	denotes	retina
T1344	4078-4081	CC	denotes	and
T1345	4082-4085	DT	denotes	the
T1346	4086-4091	NN	denotes	brain
T1347	4092-4093	-LRB-	denotes	[
T1348	4093-4095	CD	denotes	18
T1349	4095-4096	-RRB-	denotes	]
T1350	4097-4100	CC	denotes	and
T1351	4101-4107	IN	denotes	within
T1352	4108-4111	DT	denotes	the
T1353	4112-4121	NN	denotes	forebrain
T1354	4122-4123	-LRB-	denotes	[
T1356	4123-4124	CD	denotes	8
T1357	4124-4125	,	denotes	,
T1355	4125-4127	CD	denotes	19
T1358	4127-4128	-RRB-	denotes	]
T1359	4128-4129	.	denotes	.
T1360	4129-4325	sentence	denotes	It is also involved in the differentiation of neural cell types from multipotent precursors in the retina [16] and the cerebral cortex [9] through activation of bHLH genes such as Ngn2 and Mash1.
T1361	4130-4132	PRP	denotes	It
T1363	4133-4135	VBZ	denotes	is
T1364	4136-4140	RB	denotes	also
T1362	4141-4149	VBN	denotes	involved
T1365	4150-4152	IN	denotes	in
T1366	4153-4156	DT	denotes	the
T1367	4157-4172	NN	denotes	differentiation
T1368	4173-4175	IN	denotes	of
T1369	4176-4182	JJ	denotes	neural
T1371	4183-4187	NN	denotes	cell
T1370	4188-4193	NNS	denotes	types
T1372	4194-4198	IN	denotes	from
T1373	4199-4210	JJ	denotes	multipotent
T1374	4211-4221	NNS	denotes	precursors
T1375	4222-4224	IN	denotes	in
T1376	4225-4228	DT	denotes	the
T1377	4229-4235	NN	denotes	retina
T1378	4236-4237	-LRB-	denotes	[
T1379	4237-4239	CD	denotes	16
T1380	4239-4240	-RRB-	denotes	]
T1381	4241-4244	CC	denotes	and
T1382	4245-4248	DT	denotes	the
T1384	4249-4257	JJ	denotes	cerebral
T1383	4258-4264	NN	denotes	cortex
T1385	4265-4266	-LRB-	denotes	[
T1386	4266-4267	CD	denotes	9
T1387	4267-4268	-RRB-	denotes	]
T1388	4269-4276	IN	denotes	through
T1389	4277-4287	NN	denotes	activation
T1390	4288-4290	IN	denotes	of
T1391	4291-4295	NN	denotes	bHLH
T1392	4296-4301	NNS	denotes	genes
T1393	4302-4306	JJ	denotes	such
T1394	4307-4309	IN	denotes	as
T1395	4310-4314	NN	denotes	Ngn2
T1396	4315-4318	CC	denotes	and
T1397	4319-4324	NN	denotes	Mash1
T1398	4324-4325	.	denotes	.
T1399	4325-4510	sentence	denotes	These studies provide a clear link between PAX6 function in the retina and the brain, and are of particular relevance to the neurological phenotypes of individuals with PAX6 mutations.
T1400	4326-4331	DT	denotes	These
T1401	4332-4339	NNS	denotes	studies
T1402	4340-4347	VBP	denotes	provide
T1403	4348-4349	DT	denotes	a
T1405	4350-4355	JJ	denotes	clear
T1404	4356-4360	NN	denotes	link
T1406	4361-4368	IN	denotes	between
T1407	4369-4373	NN	denotes	PAX6
T1408	4374-4382	NN	denotes	function
T1409	4383-4385	IN	denotes	in
T1410	4386-4389	DT	denotes	the
T1411	4390-4396	NN	denotes	retina
T1412	4397-4400	CC	denotes	and
T1413	4401-4404	DT	denotes	the
T1414	4405-4410	NN	denotes	brain
T1415	4410-4412	,	denotes	,
T1416	4412-4415	CC	denotes	and
T1417	4416-4419	VBP	denotes	are
T1418	4420-4422	IN	denotes	of
T1419	4423-4433	JJ	denotes	particular
T1420	4434-4443	NN	denotes	relevance
T1421	4444-4446	IN	denotes	to
T1422	4447-4450	DT	denotes	the
T1424	4451-4463	JJ	denotes	neurological
T1423	4464-4474	NNS	denotes	phenotypes
T1425	4475-4477	IN	denotes	of
T1426	4478-4489	NNS	denotes	individuals
T1427	4490-4494	IN	denotes	with
T1428	4495-4499	NN	denotes	PAX6
T1429	4500-4509	NNS	denotes	mutations
T1430	4509-4510	.	denotes	.
T1431	4510-4676	sentence	denotes	It is becoming apparent that transcription factors do not act in isolation but are dependent on interactions with other proteins to carry out their function [20,21].
T1432	4511-4513	PRP	denotes	It
T1434	4514-4516	VBZ	denotes	is
T1433	4517-4525	VBG	denotes	becoming
T1435	4526-4534	JJ	denotes	apparent
T1436	4535-4539	IN	denotes	that
T1438	4540-4553	NN	denotes	transcription
T1439	4554-4561	NNS	denotes	factors
T1440	4562-4564	VBP	denotes	do
T1441	4565-4568	RB	denotes	not
T1437	4569-4572	VB	denotes	act
T1442	4573-4575	IN	denotes	in
T1443	4576-4585	NN	denotes	isolation
T1444	4586-4589	CC	denotes	but
T1445	4590-4593	VBP	denotes	are
T1446	4594-4603	JJ	denotes	dependent
T1447	4604-4606	IN	denotes	on
T1448	4607-4619	NNS	denotes	interactions
T1449	4620-4624	IN	denotes	with
T1450	4625-4630	JJ	denotes	other
T1451	4631-4639	NN	denotes	proteins
T1452	4640-4642	TO	denotes	to
T1453	4643-4648	VB	denotes	carry
T1454	4649-4652	RP	denotes	out
T1455	4653-4658	PRP$	denotes	their
T1456	4659-4667	NN	denotes	function
T1457	4668-4669	-LRB-	denotes	[
T1459	4669-4671	CD	denotes	20
T1460	4671-4672	,	denotes	,
T1458	4672-4674	CD	denotes	21
T1461	4674-4675	-RRB-	denotes	]
T1462	4675-4676	.	denotes	.
T1463	4676-4784	sentence	denotes	These interactions introduce more specificity into the regulatory function of a given transcription factor.
T1464	4677-4682	DT	denotes	These
T1465	4683-4695	NNS	denotes	interactions
T1466	4696-4705	VBP	denotes	introduce
T1467	4706-4710	JJR	denotes	more
T1468	4711-4722	NN	denotes	specificity
T1469	4723-4727	IN	denotes	into
T1470	4728-4731	DT	denotes	the
T1472	4732-4742	JJ	denotes	regulatory
T1471	4743-4751	NN	denotes	function
T1473	4752-4754	IN	denotes	of
T1474	4755-4756	DT	denotes	a
T1476	4757-4762	JJ	denotes	given
T1477	4763-4776	NN	denotes	transcription
T1475	4777-4783	NN	denotes	factor
T1478	4783-4784	.	denotes	.
T1479	4784-5134	sentence	denotes	To date only three PAX6 protein-protein interactions have been described: with SOX2 on the lens-specific enhancer element of the δ-crystallin gene [22]; with MDIA, which modulates PAX6 activity in early neuronal development [23], and with MAF proteins on the glucagon promoter, which causes increased expression of this pancreatic hormone gene [17].
T1480	4785-4787	IN	denotes	To
T1482	4788-4792	NN	denotes	date
T1483	4793-4797	RB	denotes	only
T1485	4798-4803	CD	denotes	three
T1486	4804-4808	NN	denotes	PAX6
T1487	4809-4816	NN	denotes	protein
T1489	4816-4817	SYM	denotes	-
T1488	4817-4824	NN	denotes	protein
T1484	4825-4837	NNS	denotes	interactions
T1490	4838-4842	VBP	denotes	have
T1491	4843-4847	VBN	denotes	been
T1481	4848-4857	VBN	denotes	described
T1492	4857-4859	:	denotes	:
T1493	4859-4863	IN	denotes	with
T1494	4864-4868	NN	denotes	SOX2
T1495	4869-4871	IN	denotes	on
T1496	4872-4875	DT	denotes	the
T1498	4876-4880	NN	denotes	lens
T1500	4880-4881	HYPH	denotes	-
T1499	4881-4889	JJ	denotes	specific
T1501	4890-4898	NN	denotes	enhancer
T1497	4899-4906	NN	denotes	element
T1502	4907-4909	IN	denotes	of
T1503	4910-4913	DT	denotes	the
T1505	4914-4915	SYM	denotes	δ
T1507	4915-4916	HYPH	denotes	-
T1506	4916-4926	NN	denotes	crystallin
T1504	4927-4931	NN	denotes	gene
T1508	4932-4933	-LRB-	denotes	[
T1509	4933-4935	CD	denotes	22
T1510	4935-4936	-RRB-	denotes	]
T1511	4936-4937	:	denotes	;
T1512	4938-4942	IN	denotes	with
T1513	4943-4947	NN	denotes	MDIA
T1514	4947-4949	,	denotes	,
T1515	4949-4954	WDT	denotes	which
T1516	4955-4964	VBZ	denotes	modulates
T1517	4965-4969	NN	denotes	PAX6
T1518	4970-4978	NN	denotes	activity
T1519	4979-4981	IN	denotes	in
T1520	4982-4987	JJ	denotes	early
T1522	4988-4996	JJ	denotes	neuronal
T1521	4997-5008	NN	denotes	development
T1523	5009-5010	-LRB-	denotes	[
T1524	5010-5012	CD	denotes	23
T1525	5012-5013	-RRB-	denotes	]
T1526	5013-5015	,	denotes	,
T1527	5015-5018	CC	denotes	and
T1528	5019-5023	IN	denotes	with
T1529	5024-5027	NN	denotes	MAF
T1530	5028-5036	NN	denotes	proteins
T1531	5037-5039	IN	denotes	on
T1532	5040-5043	DT	denotes	the
T1534	5044-5052	NN	denotes	glucagon
T1533	5053-5061	NN	denotes	promoter
T1535	5061-5063	,	denotes	,
T1536	5063-5068	WDT	denotes	which
T1537	5069-5075	VBZ	denotes	causes
T1538	5076-5085	VBN	denotes	increased
T1539	5086-5096	NN	denotes	expression
T1540	5097-5099	IN	denotes	of
T1541	5100-5104	DT	denotes	this
T1543	5105-5115	JJ	denotes	pancreatic
T1544	5116-5123	NN	denotes	hormone
T1542	5124-5128	NN	denotes	gene
T1545	5129-5130	-LRB-	denotes	[
T1546	5130-5132	CD	denotes	17
T1547	5132-5133	-RRB-	denotes	]
T1548	5133-5134	.	denotes	.
T1549	5134-5242	sentence	denotes	Here we report the preliminary results of the first systematic screen for proteins that interact with PAX6.
T1550	5135-5139	RB	denotes	Here
T1552	5140-5142	PRP	denotes	we
T1551	5143-5149	VBP	denotes	report
T1553	5150-5153	DT	denotes	the
T1555	5154-5165	JJ	denotes	preliminary
T1554	5166-5173	NNS	denotes	results
T1556	5174-5176	IN	denotes	of
T1557	5177-5180	DT	denotes	the
T1559	5181-5186	JJ	denotes	first
T1560	5187-5197	JJ	denotes	systematic
T1558	5198-5204	NN	denotes	screen
T1561	5205-5208	IN	denotes	for
T1562	5209-5217	NN	denotes	proteins
T1563	5218-5222	WDT	denotes	that
T1564	5223-5231	VBP	denotes	interact
T1565	5232-5236	IN	denotes	with
T1566	5237-5241	NN	denotes	PAX6
T1567	5241-5242	.	denotes	.
T1568	5242-5408	sentence	denotes	We used sequence alignment algorithms and secondary structure prediction programs to define a new domain of 32 amino acids at the C-terminal end of the PAX6 protein.
T1569	5243-5245	PRP	denotes	We
T1570	5246-5250	VBD	denotes	used
T1571	5251-5259	NN	denotes	sequence
T1572	5260-5269	NN	denotes	alignment
T1573	5270-5280	NNS	denotes	algorithms
T1574	5281-5284	CC	denotes	and
T1575	5285-5294	JJ	denotes	secondary
T1576	5295-5304	NN	denotes	structure
T1577	5305-5315	NN	denotes	prediction
T1578	5316-5324	NNS	denotes	programs
T1579	5325-5327	TO	denotes	to
T1580	5328-5334	VB	denotes	define
T1581	5335-5336	DT	denotes	a
T1583	5337-5340	JJ	denotes	new
T1582	5341-5347	NN	denotes	domain
T1584	5348-5350	IN	denotes	of
T1585	5351-5353	CD	denotes	32
T1587	5354-5359	NN	denotes	amino
T1586	5360-5365	NNS	denotes	acids
T1588	5366-5368	IN	denotes	at
T1589	5369-5372	DT	denotes	the
T1591	5373-5374	NN	denotes	C
T1593	5374-5375	HYPH	denotes	-
T1592	5375-5383	JJ	denotes	terminal
T1590	5384-5387	NN	denotes	end
T1594	5388-5390	IN	denotes	of
T1595	5391-5394	DT	denotes	the
T1597	5395-5399	NN	denotes	PAX6
T1596	5400-5407	NN	denotes	protein
T1598	5407-5408	.	denotes	.
T1599	5408-5571	sentence	denotes	We then screened a brain library with this peptide using the yeast two-hybrid technique and identified three novel interacting proteins, HOMER3, DNCL1 and TRIM11.
T1600	5409-5411	PRP	denotes	We
T1602	5412-5416	RB	denotes	then
T1601	5417-5425	VBD	denotes	screened
T1603	5426-5427	DT	denotes	a
T1605	5428-5433	NN	denotes	brain
T1604	5434-5441	NN	denotes	library
T1606	5442-5446	IN	denotes	with
T1607	5447-5451	DT	denotes	this
T1608	5452-5459	NN	denotes	peptide
T1609	5460-5465	VBG	denotes	using
T1610	5466-5469	DT	denotes	the
T1612	5470-5475	NN	denotes	yeast
T1613	5476-5479	CD	denotes	two
T1615	5479-5480	HYPH	denotes	-
T1614	5480-5486	NN	denotes	hybrid
T1611	5487-5496	NN	denotes	technique
T1616	5497-5500	CC	denotes	and
T1617	5501-5511	VBD	denotes	identified
T1618	5512-5517	CD	denotes	three
T1620	5518-5523	JJ	denotes	novel
T1621	5524-5535	VBG	denotes	interacting
T1619	5536-5544	NN	denotes	proteins
T1622	5544-5546	,	denotes	,
T1623	5546-5552	NN	denotes	HOMER3
T1624	5552-5554	,	denotes	,
T1625	5554-5559	NN	denotes	DNCL1
T1626	5560-5563	CC	denotes	and
T1627	5564-5570	NN	denotes	TRIM11
T1628	5570-5571	.	denotes	.
T1629	5571-5683	sentence	denotes	The interaction between PAX6 and these proteins was disrupted by naturally occurring C-terminal PAX6 mutations.
T1630	5572-5575	DT	denotes	The
T1631	5576-5587	NN	denotes	interaction
T1633	5588-5595	IN	denotes	between
T1634	5596-5600	NN	denotes	PAX6
T1635	5601-5604	CC	denotes	and
T1636	5605-5610	DT	denotes	these
T1637	5611-5619	NN	denotes	proteins
T1638	5620-5623	VBD	denotes	was
T1632	5624-5633	VBN	denotes	disrupted
T1639	5634-5636	IN	denotes	by
T1640	5637-5646	RB	denotes	naturally
T1641	5647-5656	VBG	denotes	occurring
T1643	5657-5658	NN	denotes	C
T1645	5658-5659	HYPH	denotes	-
T1644	5659-5667	JJ	denotes	terminal
T1646	5668-5672	NN	denotes	PAX6
T1642	5673-5682	NNS	denotes	mutations
T1647	5682-5683	.	denotes	.
T1765	5694-5695	DT	denotes	A
T1767	5696-5702	RB	denotes	highly
T1768	5703-5712	JJ	denotes	conserved
T1769	5713-5714	NN	denotes	C
T1771	5714-5715	HYPH	denotes	-
T1770	5715-5723	JJ	denotes	terminal
T1772	5724-5728	NN	denotes	PAX6
T1766	5729-5736	NN	denotes	peptide
T1773	5736-5855	sentence	denotes	We and others [31-33] noted that there is significant sequence conservation at the C-terminal end of the PAX6 protein.
T1774	5737-5739	PRP	denotes	We
T1776	5740-5743	CC	denotes	and
T1777	5744-5750	NNS	denotes	others
T1778	5751-5752	-LRB-	denotes	[
T1779	5752-5754	CD	denotes	31
T1780	5754-5755	SYM	denotes	-
T1781	5755-5757	CD	denotes	33
T1782	5757-5758	-RRB-	denotes	]
T1775	5759-5764	VBD	denotes	noted
T1783	5765-5769	IN	denotes	that
T1785	5770-5775	EX	denotes	there
T1784	5776-5778	VBZ	denotes	is
T1786	5779-5790	JJ	denotes	significant
T1788	5791-5799	NN	denotes	sequence
T1787	5800-5812	NN	denotes	conservation
T1789	5813-5815	IN	denotes	at
T1790	5816-5819	DT	denotes	the
T1792	5820-5821	NN	denotes	C
T1794	5821-5822	HYPH	denotes	-
T1793	5822-5830	JJ	denotes	terminal
T1791	5831-5834	NN	denotes	end
T1795	5835-5837	IN	denotes	of
T1796	5838-5841	DT	denotes	the
T1798	5842-5846	NN	denotes	PAX6
T1797	5847-5854	NN	denotes	protein
T1799	5854-5855	.	denotes	.
T1800	5855-6043	sentence	denotes	BLAST analysis of the amino acid sequence of the PAX6 PST domain (aa 278–422) revealed a highly conserved motif within the last 28 amino acids (beginning at the 'GLISP' motif, Figure 1a).
T1801	5856-5861	NN	denotes	BLAST
T1802	5862-5870	NN	denotes	analysis
T1804	5871-5873	IN	denotes	of
T1805	5874-5877	DT	denotes	the
T1807	5878-5883	NN	denotes	amino
T1808	5884-5888	NN	denotes	acid
T1806	5889-5897	NN	denotes	sequence
T1809	5898-5900	IN	denotes	of
T1810	5901-5904	DT	denotes	the
T1812	5905-5909	NN	denotes	PAX6
T1813	5910-5913	NN	denotes	PST
T1811	5914-5920	NN	denotes	domain
T1814	5921-5922	-LRB-	denotes	(
T1816	5922-5924	NN	denotes	aa
T1815	5925-5928	CD	denotes	278
T1817	5928-5929	SYM	denotes	–
T1818	5929-5932	CD	denotes	422
T1819	5932-5933	-RRB-	denotes	)
T1803	5934-5942	VBD	denotes	revealed
T1820	5943-5944	DT	denotes	a
T1822	5945-5951	RB	denotes	highly
T1823	5952-5961	JJ	denotes	conserved
T1821	5962-5967	NN	denotes	motif
T1824	5968-5974	IN	denotes	within
T1825	5975-5978	DT	denotes	the
T1827	5979-5983	JJ	denotes	last
T1828	5984-5986	CD	denotes	28
T1829	5987-5992	NN	denotes	amino
T1826	5993-5998	NNS	denotes	acids
T1830	5999-6000	-LRB-	denotes	(
T1831	6000-6009	VBG	denotes	beginning
T1832	6010-6012	IN	denotes	at
T1833	6013-6016	DT	denotes	the
T1835	6017-6018	``	denotes	'
T1836	6018-6023	NN	denotes	GLISP
T1837	6023-6024	''	denotes	'
T1834	6025-6030	NN	denotes	motif
T1838	6030-6032	,	denotes	,
T1839	6032-6038	NN	denotes	Figure
T1840	6039-6041	CD	denotes	1a
T1841	6041-6042	-RRB-	denotes	)
T1842	6042-6043	.	denotes	.
T1843	6043-6191	sentence	denotes	Strong conservation was seen in distantly related species such as axolotl (Ambystoma mexicanum) and sea urchin (Paracentrotus lividus) (Figure 1a).
T1844	6044-6050	JJ	denotes	Strong
T1845	6051-6063	NN	denotes	conservation
T1847	6064-6067	VBD	denotes	was
T1846	6068-6072	VBN	denotes	seen
T1848	6073-6075	IN	denotes	in
T1849	6076-6085	RB	denotes	distantly
T1850	6086-6093	VBN	denotes	related
T1851	6094-6101	NNS	denotes	species
T1852	6102-6106	JJ	denotes	such
T1853	6107-6109	IN	denotes	as
T1854	6110-6117	NN	denotes	axolotl
T1855	6118-6119	-LRB-	denotes	(
T1856	6119-6128	NNP	denotes	Ambystoma
T1857	6129-6138	NNP	denotes	mexicanum
T1858	6138-6139	-RRB-	denotes	)
T1859	6140-6143	CC	denotes	and
T1860	6144-6147	NN	denotes	sea
T1861	6148-6154	NN	denotes	urchin
T1862	6155-6156	-LRB-	denotes	(
T1863	6156-6169	NNP	denotes	Paracentrotus
T1864	6170-6177	NNP	denotes	lividus
T1865	6177-6178	-RRB-	denotes	)
T1866	6179-6180	-LRB-	denotes	(
T1867	6180-6186	NN	denotes	Figure
T1868	6187-6189	CD	denotes	1a
T1869	6189-6190	-RRB-	denotes	)
T1870	6190-6191	.	denotes	.
T1871	6191-6924	sentence	denotes	Figure 1 Characterisation of the PAX6 C-terminal peptide. (a) CLUSTAL alignment of the terminal 42 amino acids of PAX6 from diverse species. (*) indicates invariant residues, (:) indicates highly similar substitutions and (.) indicates moderately similar substitutions. (b) Secondary structure analysis of the highly conserved terminal 28 amino acids predicts a single beta sheet (arrow) in the SVPVQ peptide. (c) When 4 residues are added in the N-terminal direction, 2 beta sheets are now predicted. We subjected the whole PAX6 PST domain to secondary structure analysis using JPRED, a program that uses a number of different protein structure prediction algorithms to generate a consensus secondary structure (Figure 1) [25,26].
T8299	6202-6218	NN	denotes	Characterisation
T8300	6219-6221	IN	denotes	of
T8301	6222-6225	DT	denotes	the
T8303	6226-6230	NN	denotes	PAX6
T8304	6231-6232	NN	denotes	C
T8306	6232-6233	HYPH	denotes	-
T8305	6233-6241	JJ	denotes	terminal
T8302	6242-6249	NN	denotes	peptide
T8307	6249-6250	.	denotes	.
T8308	6250-6333	sentence	denotes	(a) CLUSTAL alignment of the terminal 42 amino acids of PAX6 from diverse species.
T8309	6251-6252	-LRB-	denotes	(
T8310	6252-6253	LS	denotes	a
T8312	6253-6254	-RRB-	denotes	)
T8313	6255-6262	NNP	denotes	CLUSTAL
T8311	6263-6272	NN	denotes	alignment
T8314	6273-6275	IN	denotes	of
T8315	6276-6279	DT	denotes	the
T8317	6280-6288	JJ	denotes	terminal
T8318	6289-6291	CD	denotes	42
T8319	6292-6297	NN	denotes	amino
T8316	6298-6303	NNS	denotes	acids
T8320	6304-6306	IN	denotes	of
T8321	6307-6311	NN	denotes	PAX6
T8322	6312-6316	IN	denotes	from
T8323	6317-6324	JJ	denotes	diverse
T8324	6325-6332	NNS	denotes	species
T8325	6332-6333	.	denotes	.
T8326	6333-6462	sentence	denotes	(*) indicates invariant residues, (:) indicates highly similar substitutions and (.) indicates moderately similar substitutions.
T8327	6334-6335	-LRB-	denotes	(
T8329	6335-6336	SYM	denotes	*
T8330	6336-6337	-RRB-	denotes	)
T8328	6338-6347	VBZ	denotes	indicates
T8332	6348-6357	JJ	denotes	invariant
T8333	6358-6366	NNS	denotes	residues
T8334	6366-6368	,	denotes	,
T8335	6368-6369	-LRB-	denotes	(
T8336	6369-6370	SYM	denotes	:
T8337	6370-6371	-RRB-	denotes	)
T8331	6372-6381	VBZ	denotes	indicates
T8338	6382-6388	RB	denotes	highly
T8339	6389-6396	JJ	denotes	similar
T8340	6397-6410	NNS	denotes	substitutions
T8341	6411-6414	CC	denotes	and
T8342	6415-6416	-LRB-	denotes	(
T8344	6416-6417	SYM	denotes	.
T8345	6417-6418	-RRB-	denotes	)
T8343	6419-6428	VBZ	denotes	indicates
T8346	6429-6439	RB	denotes	moderately
T8347	6440-6447	JJ	denotes	similar
T8348	6448-6461	NNS	denotes	substitutions
T8349	6461-6462	.	denotes	.
T8350	6462-6602	sentence	denotes	(b) Secondary structure analysis of the highly conserved terminal 28 amino acids predicts a single beta sheet (arrow) in the SVPVQ peptide.
T8351	6463-6464	-LRB-	denotes	(
T8352	6464-6465	LS	denotes	b
T8354	6465-6466	-RRB-	denotes	)
T8355	6467-6476	JJ	denotes	Secondary
T8357	6477-6486	NN	denotes	structure
T8356	6487-6495	NN	denotes	analysis
T8358	6496-6498	IN	denotes	of
T8359	6499-6502	DT	denotes	the
T8361	6503-6509	RB	denotes	highly
T8362	6510-6519	JJ	denotes	conserved
T8363	6520-6528	JJ	denotes	terminal
T8364	6529-6531	CD	denotes	28
T8365	6532-6537	NN	denotes	amino
T8360	6538-6543	NNS	denotes	acids
T8353	6544-6552	VBZ	denotes	predicts
T8366	6553-6554	DT	denotes	a
T8368	6555-6561	JJ	denotes	single
T8369	6562-6566	NN	denotes	beta
T8367	6567-6572	NN	denotes	sheet
T8370	6573-6574	-LRB-	denotes	(
T8371	6574-6579	NN	denotes	arrow
T8372	6579-6580	-RRB-	denotes	)
T8373	6581-6583	IN	denotes	in
T8374	6584-6587	DT	denotes	the
T8376	6588-6593	NN	denotes	SVPVQ
T8375	6594-6601	NN	denotes	peptide
T8377	6601-6602	.	denotes	.
T8378	6602-6694	sentence	denotes	(c) When 4 residues are added in the N-terminal direction, 2 beta sheets are now predicted.
T8379	6603-6604	-LRB-	denotes	(
T8380	6604-6605	LS	denotes	c
T8382	6605-6606	-RRB-	denotes	)
T8383	6607-6611	WRB	denotes	When
T8385	6612-6613	CD	denotes	4
T8386	6614-6622	NNS	denotes	residues
T8387	6623-6626	VBP	denotes	are
T8384	6627-6632	VBN	denotes	added
T8388	6633-6635	IN	denotes	in
T8389	6636-6639	DT	denotes	the
T8391	6640-6641	NN	denotes	N
T8393	6641-6642	HYPH	denotes	-
T8392	6642-6650	JJ	denotes	terminal
T8390	6651-6660	NN	denotes	direction
T8394	6660-6662	,	denotes	,
T8395	6662-6663	CD	denotes	2
T8397	6664-6668	NN	denotes	beta
T8396	6669-6675	NNS	denotes	sheets
T8398	6676-6679	VBP	denotes	are
T8399	6680-6683	RB	denotes	now
T8381	6684-6693	VBN	denotes	predicted
T8400	6693-6694	.	denotes	.
T1872	6695-6697	PRP	denotes	We
T1873	6698-6707	VBD	denotes	subjected
T1874	6708-6711	DT	denotes	the
T1876	6712-6717	JJ	denotes	whole
T1877	6718-6722	NN	denotes	PAX6
T1878	6723-6726	NN	denotes	PST
T1875	6727-6733	NN	denotes	domain
T1879	6734-6736	IN	denotes	to
T1880	6737-6746	JJ	denotes	secondary
T1882	6747-6756	NN	denotes	structure
T1881	6757-6765	NN	denotes	analysis
T1883	6766-6771	VBG	denotes	using
T1884	6772-6777	NNP	denotes	JPRED
T1885	6777-6779	,	denotes	,
T1886	6779-6780	DT	denotes	a
T1887	6781-6788	NN	denotes	program
T1888	6789-6793	WDT	denotes	that
T1889	6794-6798	VBZ	denotes	uses
T1890	6799-6800	DT	denotes	a
T1891	6801-6807	NN	denotes	number
T1892	6808-6810	IN	denotes	of
T1893	6811-6820	JJ	denotes	different
T1895	6821-6828	NN	denotes	protein
T1896	6829-6838	NN	denotes	structure
T1897	6839-6849	NN	denotes	prediction
T1894	6850-6860	NNS	denotes	algorithms
T1898	6861-6863	TO	denotes	to
T1899	6864-6872	VB	denotes	generate
T1900	6873-6874	DT	denotes	a
T1902	6875-6884	NN	denotes	consensus
T1903	6885-6894	JJ	denotes	secondary
T1901	6895-6904	NN	denotes	structure
T1904	6905-6906	-LRB-	denotes	(
T1905	6906-6912	NN	denotes	Figure
T1906	6913-6914	CD	denotes	1
T1907	6914-6915	-RRB-	denotes	)
T1908	6916-6917	-LRB-	denotes	[
T1910	6917-6919	CD	denotes	25
T1911	6919-6920	,	denotes	,
T1909	6920-6922	CD	denotes	26
T1912	6922-6923	-RRB-	denotes	]
T1913	6923-6924	.	denotes	.
T1914	6924-7204	sentence	denotes	The PST domain was largely devoid of predicted secondary structure except for the C-terminal region, which contained two predicted beta sheets within the highly conserved domain, one in the 'GLISP' motif and one in the 'SPVPQ' motif (identical to the pattern shown in Figure 1c).
T1915	6925-6928	DT	denotes	The
T1917	6929-6932	NN	denotes	PST
T1916	6933-6939	NN	denotes	domain
T1918	6940-6943	VBD	denotes	was
T1919	6944-6951	RB	denotes	largely
T1920	6952-6958	JJ	denotes	devoid
T1921	6959-6961	IN	denotes	of
T1922	6962-6971	VBN	denotes	predicted
T1924	6972-6981	JJ	denotes	secondary
T1923	6982-6991	NN	denotes	structure
T1925	6992-6998	IN	denotes	except
T1926	6999-7002	IN	denotes	for
T1927	7003-7006	DT	denotes	the
T1929	7007-7008	NN	denotes	C
T1931	7008-7009	HYPH	denotes	-
T1930	7009-7017	JJ	denotes	terminal
T1928	7018-7024	NN	denotes	region
T1932	7024-7026	,	denotes	,
T1933	7026-7031	WDT	denotes	which
T1934	7032-7041	VBD	denotes	contained
T1935	7042-7045	CD	denotes	two
T1937	7046-7055	VBN	denotes	predicted
T1938	7056-7060	NN	denotes	beta
T1936	7061-7067	NNS	denotes	sheets
T1939	7068-7074	IN	denotes	within
T1940	7075-7078	DT	denotes	the
T1942	7079-7085	RB	denotes	highly
T1943	7086-7095	JJ	denotes	conserved
T1941	7096-7102	NN	denotes	domain
T1944	7102-7104	,	denotes	,
T1945	7104-7107	CD	denotes	one
T1946	7108-7110	IN	denotes	in
T1947	7111-7114	DT	denotes	the
T1949	7115-7116	``	denotes	'
T1948	7116-7121	NN	denotes	GLISP
T1951	7121-7122	''	denotes	'
T1950	7123-7128	NN	denotes	motif
T1952	7129-7132	CC	denotes	and
T1953	7133-7136	CD	denotes	one
T1954	7137-7139	IN	denotes	in
T1955	7140-7143	DT	denotes	the
T1957	7144-7145	``	denotes	'
T1956	7145-7150	NN	denotes	SPVPQ
T1959	7150-7151	''	denotes	'
T1958	7152-7157	NN	denotes	motif
T1960	7158-7159	-LRB-	denotes	(
T1961	7159-7168	JJ	denotes	identical
T1962	7169-7171	IN	denotes	to
T1963	7172-7175	DT	denotes	the
T1964	7176-7183	NN	denotes	pattern
T1965	7184-7189	VBN	denotes	shown
T1966	7190-7192	IN	denotes	in
T1967	7193-7199	NN	denotes	Figure
T1968	7200-7202	CD	denotes	1c
T1969	7202-7203	-RRB-	denotes	)
T1970	7203-7204	.	denotes	.
T1971	7204-7410	sentence	denotes	Initially we defined the C-terminal domain as running from the 'GLISP' motif up to the stop codon, since this region was most highly conserved and contained strongly predicted secondary structure elements.
T1972	7205-7214	RB	denotes	Initially
T1974	7215-7217	PRP	denotes	we
T1973	7218-7225	VBD	denotes	defined
T1975	7226-7229	DT	denotes	the
T1977	7230-7231	NN	denotes	C
T1979	7231-7232	HYPH	denotes	-
T1978	7232-7240	JJ	denotes	terminal
T1976	7241-7247	NN	denotes	domain
T1980	7248-7250	IN	denotes	as
T1981	7251-7258	VBG	denotes	running
T1982	7259-7263	IN	denotes	from
T1983	7264-7267	DT	denotes	the
T1985	7268-7269	``	denotes	'
T1984	7269-7274	NN	denotes	GLISP
T1987	7274-7275	''	denotes	'
T1986	7276-7281	NN	denotes	motif
T1988	7282-7284	IN	denotes	up
T1989	7285-7287	IN	denotes	to
T1990	7288-7291	DT	denotes	the
T1992	7292-7296	NN	denotes	stop
T1991	7297-7302	NN	denotes	codon
T1993	7302-7304	,	denotes	,
T1994	7304-7309	IN	denotes	since
T1996	7310-7314	DT	denotes	this
T1997	7315-7321	NN	denotes	region
T1998	7322-7325	VBD	denotes	was
T1999	7326-7330	RBS	denotes	most
T2000	7331-7337	RB	denotes	highly
T1995	7338-7347	VBN	denotes	conserved
T2001	7348-7351	CC	denotes	and
T2002	7352-7361	VBD	denotes	contained
T2003	7362-7370	RB	denotes	strongly
T2004	7371-7380	VBN	denotes	predicted
T2006	7381-7390	JJ	denotes	secondary
T2007	7391-7400	NN	denotes	structure
T2005	7401-7409	NNS	denotes	elements
T2008	7409-7410	.	denotes	.
T2009	7410-7550	sentence	denotes	However when we performed secondary structure prediction analysis on this 28 amino acid peptide, the first beta sheet was lost (Figure 1b).
T2010	7411-7418	RB	denotes	However
T2012	7419-7423	WRB	denotes	when
T2014	7424-7426	PRP	denotes	we
T2013	7427-7436	VBD	denotes	performed
T2015	7437-7446	JJ	denotes	secondary
T2016	7447-7456	NN	denotes	structure
T2017	7457-7467	NN	denotes	prediction
T2018	7468-7476	NN	denotes	analysis
T2019	7477-7479	IN	denotes	on
T2020	7480-7484	DT	denotes	this
T2022	7485-7487	CD	denotes	28
T2024	7488-7493	NN	denotes	amino
T2023	7494-7498	NN	denotes	acid
T2021	7499-7506	NN	denotes	peptide
T2025	7506-7508	,	denotes	,
T2026	7508-7511	DT	denotes	the
T2028	7512-7517	JJ	denotes	first
T2029	7518-7522	NN	denotes	beta
T2027	7523-7528	NN	denotes	sheet
T2030	7529-7532	VBD	denotes	was
T2011	7533-7537	VBN	denotes	lost
T2031	7538-7539	-LRB-	denotes	(
T2032	7539-7545	NN	denotes	Figure
T2033	7546-7548	CD	denotes	1b
T2034	7548-7549	-RRB-	denotes	)
T2035	7549-7550	.	denotes	.
T2036	7550-7671	sentence	denotes	Addition of another 4 amino acids (TTST) immediately before 'GLISP' caused recovery of the first beta sheet (Figure 1c).
T2037	7551-7559	NN	denotes	Addition
T2039	7560-7562	IN	denotes	of
T2040	7563-7570	DT	denotes	another
T2042	7571-7572	CD	denotes	4
T2043	7573-7578	NN	denotes	amino
T2041	7579-7584	NNS	denotes	acids
T2044	7585-7586	-LRB-	denotes	(
T2045	7586-7590	NN	denotes	TTST
T2046	7590-7591	-RRB-	denotes	)
T2047	7592-7603	RB	denotes	immediately
T2048	7604-7610	IN	denotes	before
T2049	7611-7612	``	denotes	'
T2050	7612-7617	NN	denotes	GLISP
T2051	7617-7618	''	denotes	'
T2038	7619-7625	VBN	denotes	caused
T2052	7626-7634	NN	denotes	recovery
T2053	7635-7637	IN	denotes	of
T2054	7638-7641	DT	denotes	the
T2056	7642-7647	JJ	denotes	first
T2057	7648-7652	NN	denotes	beta
T2055	7653-7658	NN	denotes	sheet
T2058	7659-7660	-LRB-	denotes	(
T2059	7660-7666	NN	denotes	Figure
T2060	7667-7669	CD	denotes	1c
T2061	7669-7670	-RRB-	denotes	)
T2062	7670-7671	.	denotes	.
T2063	7671-7835	sentence	denotes	Although these 4 residues are not highly conserved (Figure 1a), they appear to be important for seeding the first beta sheet and therefore for secondary structure.
T2064	7672-7680	IN	denotes	Although
T2066	7681-7686	DT	denotes	these
T2068	7687-7688	CD	denotes	4
T2067	7689-7697	NNS	denotes	residues
T2065	7698-7701	VBP	denotes	are
T2070	7702-7705	RB	denotes	not
T2071	7706-7712	RB	denotes	highly
T2072	7713-7722	VBN	denotes	conserved
T2073	7723-7724	-LRB-	denotes	(
T2074	7724-7730	NN	denotes	Figure
T2075	7731-7733	CD	denotes	1a
T2076	7733-7734	-RRB-	denotes	)
T2077	7734-7736	,	denotes	,
T2078	7736-7740	PRP	denotes	they
T2069	7741-7747	VBP	denotes	appear
T2079	7748-7750	TO	denotes	to
T2080	7751-7753	VB	denotes	be
T2081	7754-7763	JJ	denotes	important
T2082	7764-7767	IN	denotes	for
T2083	7768-7775	VBG	denotes	seeding
T2084	7776-7779	DT	denotes	the
T2086	7780-7785	JJ	denotes	first
T2087	7786-7790	NN	denotes	beta
T2085	7791-7796	NN	denotes	sheet
T2088	7797-7800	CC	denotes	and
T2089	7801-7810	RB	denotes	therefore
T2090	7811-7814	IN	denotes	for
T2091	7815-7824	JJ	denotes	secondary
T2092	7825-7834	NN	denotes	structure
T2093	7834-7835	.	denotes	.
T2094	7835-7986	sentence	denotes	Thus we define the C-terminal peptide as being the final 32 amino acids of PAX6, running from threonine 391 to the stop codon (top line of Figure 1c).
T2095	7836-7840	RB	denotes	Thus
T2097	7841-7843	PRP	denotes	we
T2096	7844-7850	VBP	denotes	define
T2098	7851-7854	DT	denotes	the
T2100	7855-7856	NN	denotes	C
T2102	7856-7857	HYPH	denotes	-
T2101	7857-7865	JJ	denotes	terminal
T2099	7866-7873	NN	denotes	peptide
T2103	7874-7876	IN	denotes	as
T2104	7877-7882	VBG	denotes	being
T2105	7883-7886	DT	denotes	the
T2107	7887-7892	JJ	denotes	final
T2108	7893-7895	CD	denotes	32
T2109	7896-7901	NN	denotes	amino
T2106	7902-7907	NNS	denotes	acids
T2110	7908-7910	IN	denotes	of
T2111	7911-7915	NN	denotes	PAX6
T2112	7915-7917	,	denotes	,
T2113	7917-7924	VBG	denotes	running
T2114	7925-7929	IN	denotes	from
T2115	7930-7939	NN	denotes	threonine
T2116	7940-7943	CD	denotes	391
T2117	7944-7946	IN	denotes	to
T2118	7947-7950	DT	denotes	the
T2120	7951-7955	NN	denotes	stop
T2119	7956-7961	NN	denotes	codon
T2121	7962-7963	-LRB-	denotes	(
T2123	7963-7966	NN	denotes	top
T2122	7967-7971	NN	denotes	line
T2124	7972-7974	IN	denotes	of
T2125	7975-7981	NN	denotes	Figure
T2126	7982-7984	CD	denotes	1c
T2127	7984-7985	-RRB-	denotes	)
T2128	7985-7986	.	denotes	.
T2344	7988-7993	NN	denotes	Yeast
T2346	7994-7997	CD	denotes	two
T2348	7997-7998	HYPH	denotes	-
T2347	7998-8004	NN	denotes	hybrid
T2345	8005-8014	NN	denotes	screening
T2349	8014-8311	sentence	denotes	We hypothesised that the C-terminal peptide might be involved in protein-protein interactions, and we tested this by screening a cDNA library using the yeast two-hybrid system with a construct (PAX6CTP) in which the 32 amino acid C-terminal peptide was fused to the yeast GAL4 DNA binding domain.
T2350	8015-8017	PRP	denotes	We
T2351	8018-8030	VBD	denotes	hypothesised
T2352	8031-8035	IN	denotes	that
T2354	8036-8039	DT	denotes	the
T2356	8040-8041	NN	denotes	C
T2358	8041-8042	HYPH	denotes	-
T2357	8042-8050	JJ	denotes	terminal
T2355	8051-8058	NN	denotes	peptide
T2359	8059-8064	MD	denotes	might
T2360	8065-8067	VB	denotes	be
T2353	8068-8076	VBN	denotes	involved
T2361	8077-8079	IN	denotes	in
T2362	8080-8087	NN	denotes	protein
T2364	8087-8088	SYM	denotes	-
T2363	8088-8095	NN	denotes	protein
T2365	8096-8108	NNS	denotes	interactions
T2366	8108-8110	,	denotes	,
T2367	8110-8113	CC	denotes	and
T2368	8114-8116	PRP	denotes	we
T2369	8117-8123	VBD	denotes	tested
T2370	8124-8128	DT	denotes	this
T2371	8129-8131	IN	denotes	by
T2372	8132-8141	VBG	denotes	screening
T2373	8142-8143	DT	denotes	a
T2375	8144-8148	NN	denotes	cDNA
T2374	8149-8156	NN	denotes	library
T2376	8157-8162	VBG	denotes	using
T2377	8163-8166	DT	denotes	the
T2379	8167-8172	NN	denotes	yeast
T2380	8173-8176	CD	denotes	two
T2382	8176-8177	HYPH	denotes	-
T2381	8177-8183	NN	denotes	hybrid
T2378	8184-8190	NN	denotes	system
T2383	8191-8195	IN	denotes	with
T2384	8196-8197	DT	denotes	a
T2385	8198-8207	NN	denotes	construct
T2386	8208-8209	-LRB-	denotes	(
T2387	8209-8216	NN	denotes	PAX6CTP
T2388	8216-8217	-RRB-	denotes	)
T2389	8218-8220	IN	denotes	in
T2391	8221-8226	WDT	denotes	which
T2392	8227-8230	DT	denotes	the
T2394	8231-8233	CD	denotes	32
T2396	8234-8239	NN	denotes	amino
T2395	8240-8244	NN	denotes	acid
T2397	8245-8246	NN	denotes	C
T2399	8246-8247	HYPH	denotes	-
T2398	8247-8255	JJ	denotes	terminal
T2393	8256-8263	NN	denotes	peptide
T2400	8264-8267	VBD	denotes	was
T2390	8268-8273	VBN	denotes	fused
T2401	8274-8276	IN	denotes	to
T2402	8277-8280	DT	denotes	the
T2404	8281-8286	NN	denotes	yeast
T2405	8287-8291	NN	denotes	GAL4
T2406	8292-8295	NN	denotes	DNA
T2407	8296-8303	NN	denotes	binding
T2403	8304-8310	NN	denotes	domain
T2408	8310-8311	.	denotes	.
T2409	8311-8395	sentence	denotes	We chose to screen a mouse brain cDNA library as no human libraries were available.
T2410	8312-8314	PRP	denotes	We
T2411	8315-8320	VBD	denotes	chose
T2412	8321-8323	TO	denotes	to
T2413	8324-8330	VB	denotes	screen
T2414	8331-8332	DT	denotes	a
T2416	8333-8338	NN	denotes	mouse
T2417	8339-8344	NN	denotes	brain
T2418	8345-8349	NN	denotes	cDNA
T2415	8350-8357	NN	denotes	library
T2419	8358-8360	IN	denotes	as
T2421	8361-8363	DT	denotes	no
T2423	8364-8369	JJ	denotes	human
T2422	8370-8379	NNS	denotes	libraries
T2420	8380-8384	VBD	denotes	were
T2424	8385-8394	JJ	denotes	available
T2425	8394-8395	.	denotes	.
T2426	8395-8563	sentence	denotes	Given the fact that the amino acid sequence of the PAX6 protein is identical in man and mouse, we reasoned that a mouse brain library would yield relevant interactors.
T2427	8396-8401	VBN	denotes	Given
T2429	8402-8405	DT	denotes	the
T2430	8406-8410	NN	denotes	fact
T2431	8411-8415	IN	denotes	that
T2433	8416-8419	DT	denotes	the
T2435	8420-8425	NN	denotes	amino
T2436	8426-8430	NN	denotes	acid
T2434	8431-8439	NN	denotes	sequence
T2437	8440-8442	IN	denotes	of
T2438	8443-8446	DT	denotes	the
T2440	8447-8451	NN	denotes	PAX6
T2439	8452-8459	NN	denotes	protein
T2432	8460-8462	VBZ	denotes	is
T2441	8463-8472	JJ	denotes	identical
T2442	8473-8475	IN	denotes	in
T2443	8476-8479	NN	denotes	man
T2444	8480-8483	CC	denotes	and
T2445	8484-8489	NN	denotes	mouse
T2446	8489-8491	,	denotes	,
T2447	8491-8493	PRP	denotes	we
T2428	8494-8502	VBD	denotes	reasoned
T2448	8503-8507	IN	denotes	that
T2450	8508-8509	DT	denotes	a
T2452	8510-8515	NN	denotes	mouse
T2453	8516-8521	NN	denotes	brain
T2451	8522-8529	NN	denotes	library
T2454	8530-8535	MD	denotes	would
T2449	8536-8541	VB	denotes	yield
T2455	8542-8550	JJ	denotes	relevant
T2456	8551-8562	NNS	denotes	interactors
T2457	8562-8563	.	denotes	.
T2458	8563-8670	sentence	denotes	For comparison we also carried out the screen using a construct containing the whole PST domain (PAX6PST).
T2459	8564-8567	IN	denotes	For
T2461	8568-8578	NN	denotes	comparison
T2462	8579-8581	PRP	denotes	we
T2463	8582-8586	RB	denotes	also
T2460	8587-8594	VBD	denotes	carried
T2464	8595-8598	RP	denotes	out
T2465	8599-8602	DT	denotes	the
T2466	8603-8609	NN	denotes	screen
T2467	8610-8615	VBG	denotes	using
T2468	8616-8617	DT	denotes	a
T2469	8618-8627	NN	denotes	construct
T2470	8628-8638	VBG	denotes	containing
T2471	8639-8642	DT	denotes	the
T2473	8643-8648	JJ	denotes	whole
T2474	8649-8652	NN	denotes	PST
T2472	8653-8659	NN	denotes	domain
T2475	8660-8661	-LRB-	denotes	(
T2476	8661-8668	NN	denotes	PAX6PST
T2477	8668-8669	-RRB-	denotes	)
T2478	8669-8670	.	denotes	.
T2479	8670-8805	sentence	denotes	The C-terminal peptide screen gave 15 colonies that were positive with all three reporters and the PST domain screen gave 62 colonies.
T2480	8671-8674	DT	denotes	The
T2482	8675-8676	NN	denotes	C
T2484	8676-8677	HYPH	denotes	-
T2483	8677-8685	JJ	denotes	terminal
T2485	8686-8693	NN	denotes	peptide
T2481	8694-8700	NN	denotes	screen
T2486	8701-8705	VBD	denotes	gave
T2487	8706-8708	CD	denotes	15
T2488	8709-8717	NNS	denotes	colonies
T2489	8718-8722	WDT	denotes	that
T2490	8723-8727	VBD	denotes	were
T2491	8728-8736	JJ	denotes	positive
T2492	8737-8741	IN	denotes	with
T2493	8742-8745	DT	denotes	all
T2495	8746-8751	CD	denotes	three
T2494	8752-8761	NNS	denotes	reporters
T2496	8762-8765	CC	denotes	and
T2497	8766-8769	DT	denotes	the
T2499	8770-8773	NN	denotes	PST
T2500	8774-8780	NN	denotes	domain
T2498	8781-8787	NN	denotes	screen
T2501	8788-8792	VBD	denotes	gave
T2502	8793-8795	CD	denotes	62
T2503	8796-8804	NNS	denotes	colonies
T2504	8804-8805	.	denotes	.
T2505	8805-8876	sentence	denotes	The interacting plasmids were isolated and the cDNA inserts sequenced.
T2506	8806-8809	DT	denotes	The
T2508	8810-8821	VBG	denotes	interacting
T2507	8822-8830	NNS	denotes	plasmids
T2510	8831-8835	VBD	denotes	were
T2509	8836-8844	VBN	denotes	isolated
T2511	8845-8848	CC	denotes	and
T2512	8849-8852	DT	denotes	the
T2514	8853-8857	NN	denotes	cDNA
T2513	8858-8865	NNS	denotes	inserts
T2515	8866-8875	VBN	denotes	sequenced
T2516	8875-8876	.	denotes	.
T2517	8876-9087	sentence	denotes	Three cDNAs were identified 3 or more times, Homer3 (NM_011984), Dncl1 (Dynein cytoplasmic light chain 1, NM_019682, also known as Pin or Dlc8) and Trim11 (Tripartite motif protein family member 11, NM_053168).
T2518	8877-8882	CD	denotes	Three
T2519	8883-8888	NNS	denotes	cDNAs
T2521	8889-8893	VBD	denotes	were
T2520	8894-8904	VBN	denotes	identified
T2522	8905-8906	CD	denotes	3
T2524	8907-8909	CC	denotes	or
T2525	8910-8914	JJR	denotes	more
T2523	8915-8920	NNS	denotes	times
T2526	8920-8922	,	denotes	,
T2527	8922-8928	NN	denotes	Homer3
T2528	8929-8930	-LRB-	denotes	(
T2529	8930-8939	NN	denotes	NM_011984
T2530	8939-8940	-RRB-	denotes	)
T2531	8940-8942	,	denotes	,
T2532	8942-8947	NN	denotes	Dncl1
T2533	8948-8949	-LRB-	denotes	(
T2534	8949-8955	NNP	denotes	Dynein
T2536	8956-8967	JJ	denotes	cytoplasmic
T2537	8968-8973	JJ	denotes	light
T2535	8974-8979	NN	denotes	chain
T2538	8980-8981	CD	denotes	1
T2539	8981-8983	,	denotes	,
T2540	8983-8992	NN	denotes	NM_019682
T2541	8992-8994	,	denotes	,
T2542	8994-8998	RB	denotes	also
T2543	8999-9004	VBN	denotes	known
T2544	9005-9007	IN	denotes	as
T2545	9008-9011	NN	denotes	Pin
T2546	9012-9014	CC	denotes	or
T2547	9015-9019	NN	denotes	Dlc8
T2548	9019-9020	-RRB-	denotes	)
T2549	9021-9024	CC	denotes	and
T2550	9025-9031	NN	denotes	Trim11
T2551	9032-9033	-LRB-	denotes	(
T2552	9033-9043	JJ	denotes	Tripartite
T2553	9044-9049	NN	denotes	motif
T2555	9050-9057	NN	denotes	protein
T2556	9058-9064	NN	denotes	family
T2554	9065-9071	NN	denotes	member
T2557	9072-9074	CD	denotes	11
T2558	9074-9076	,	denotes	,
T2559	9076-9085	NN	denotes	NM_053168
T2560	9085-9086	-RRB-	denotes	)
T2561	9086-9087	.	denotes	.
T2562	9087-9176	sentence	denotes	Homer3 (6 clones) and Dncl1 (2 clones) were identified in the C-terminal peptide screen.
T2563	9088-9094	NN	denotes	Homer3
T2565	9095-9096	-LRB-	denotes	(
T2567	9096-9097	CD	denotes	6
T2566	9098-9104	NNS	denotes	clones
T2568	9104-9105	-RRB-	denotes	)
T2569	9106-9109	CC	denotes	and
T2570	9110-9115	NN	denotes	Dncl1
T2571	9116-9117	-LRB-	denotes	(
T2573	9117-9118	CD	denotes	2
T2572	9119-9125	NNS	denotes	clones
T2574	9125-9126	-RRB-	denotes	)
T2575	9127-9131	VBD	denotes	were
T2564	9132-9142	VBN	denotes	identified
T2576	9143-9145	IN	denotes	in
T2577	9146-9149	DT	denotes	the
T2579	9150-9151	NN	denotes	C
T2581	9151-9152	HYPH	denotes	-
T2580	9152-9160	JJ	denotes	terminal
T2582	9161-9168	NN	denotes	peptide
T2578	9169-9175	NN	denotes	screen
T2583	9175-9176	.	denotes	.
T2584	9176-9276	sentence	denotes	Homer3 (7 clones), Dncl1 (2 clones) and Trim11 (6 clones) were identified in the PST domain screen.
T2585	9177-9183	NN	denotes	Homer3
T2587	9184-9185	-LRB-	denotes	(
T2589	9185-9186	CD	denotes	7
T2588	9187-9193	NNS	denotes	clones
T2590	9193-9194	-RRB-	denotes	)
T2591	9194-9196	,	denotes	,
T2592	9196-9201	NN	denotes	Dncl1
T2593	9202-9203	-LRB-	denotes	(
T2595	9203-9204	CD	denotes	2
T2594	9205-9211	NNS	denotes	clones
T2596	9211-9212	-RRB-	denotes	)
T2597	9213-9216	CC	denotes	and
T2598	9217-9223	NN	denotes	Trim11
T2599	9224-9225	-LRB-	denotes	(
T2601	9225-9226	CD	denotes	6
T2600	9227-9233	NNS	denotes	clones
T2602	9233-9234	-RRB-	denotes	)
T2603	9235-9239	VBD	denotes	were
T2586	9240-9250	VBN	denotes	identified
T2604	9251-9253	IN	denotes	in
T2605	9254-9257	DT	denotes	the
T2607	9258-9261	NN	denotes	PST
T2608	9262-9268	NN	denotes	domain
T2606	9269-9275	NN	denotes	screen
T2609	9275-9276	.	denotes	.
T2610	9276-9367	sentence	denotes	All cDNA inserts were in-frame with the coding region of the pPC86 GAL4 activation domain.
T2611	9277-9280	DT	denotes	All
T2613	9281-9285	NN	denotes	cDNA
T2612	9286-9293	NNS	denotes	inserts
T2614	9294-9298	VBD	denotes	were
T2615	9299-9301	IN	denotes	in
T2616	9301-9302	HYPH	denotes	-
T2617	9302-9307	NN	denotes	frame
T2618	9308-9312	IN	denotes	with
T2619	9313-9316	DT	denotes	the
T2621	9317-9323	NN	denotes	coding
T2620	9324-9330	NN	denotes	region
T2622	9331-9333	IN	denotes	of
T2623	9334-9337	DT	denotes	the
T2625	9338-9343	NN	denotes	pPC86
T2626	9344-9348	NN	denotes	GAL4
T2627	9349-9359	NN	denotes	activation
T2624	9360-9366	NN	denotes	domain
T2628	9366-9367	.	denotes	.
T2629	9367-9438	sentence	denotes	None of the cDNAs was present in a list of known false positives [34].
T2630	9368-9372	NN	denotes	None
T2632	9373-9375	IN	denotes	of
T2633	9376-9379	DT	denotes	the
T2634	9380-9385	NNS	denotes	cDNAs
T2631	9386-9389	VBD	denotes	was
T2635	9390-9397	JJ	denotes	present
T2636	9398-9400	IN	denotes	in
T2637	9401-9402	DT	denotes	a
T2638	9403-9407	NN	denotes	list
T2639	9408-9410	IN	denotes	of
T2640	9411-9416	JJ	denotes	known
T2642	9417-9422	JJ	denotes	false
T2641	9423-9432	NNS	denotes	positives
T2643	9433-9434	-LRB-	denotes	[
T2644	9434-9436	CD	denotes	34
T2645	9436-9437	-RRB-	denotes	]
T2646	9437-9438	.	denotes	.
T2647	9438-9532	sentence	denotes	HOMER3 is a member of the HOMER family of neuronal post-synaptic density (PSD) proteins [35].
T2648	9439-9445	NN	denotes	HOMER3
T2649	9446-9448	VBZ	denotes	is
T2650	9449-9450	DT	denotes	a
T2651	9451-9457	NN	denotes	member
T2652	9458-9460	IN	denotes	of
T2653	9461-9464	DT	denotes	the
T2655	9465-9470	NN	denotes	HOMER
T2654	9471-9477	NN	denotes	family
T2656	9478-9480	IN	denotes	of
T2657	9481-9489	JJ	denotes	neuronal
T2659	9490-9503	JJ	denotes	post-synaptic
T2660	9504-9511	NN	denotes	density
T2661	9512-9513	-LRB-	denotes	(
T2662	9513-9516	NN	denotes	PSD
T2663	9516-9517	-RRB-	denotes	)
T2658	9518-9526	NN	denotes	proteins
T2664	9527-9528	-LRB-	denotes	[
T2665	9528-9530	CD	denotes	35
T2666	9530-9531	-RRB-	denotes	]
T2667	9531-9532	.	denotes	.
T2668	9532-9708	sentence	denotes	DNCL1 is a subunit of two motor protein complexes, dynein and myosin-Va, both of which are involved in intracellular trafficking of proteins and organelles in neurons [36,37].
T2669	9533-9538	NN	denotes	DNCL1
T2670	9539-9541	VBZ	denotes	is
T2671	9542-9543	DT	denotes	a
T2672	9544-9551	NN	denotes	subunit
T2673	9552-9554	IN	denotes	of
T2674	9555-9558	CD	denotes	two
T2676	9559-9564	NN	denotes	motor
T2677	9565-9572	NN	denotes	protein
T2675	9573-9582	NNS	denotes	complexes
T2678	9582-9584	,	denotes	,
T2679	9584-9590	NN	denotes	dynein
T2680	9591-9594	CC	denotes	and
T2681	9595-9601	NN	denotes	myosin
T2683	9601-9602	HYPH	denotes	-
T2682	9602-9604	NN	denotes	Va
T2684	9604-9606	,	denotes	,
T2685	9606-9610	DT	denotes	both
T2687	9611-9613	IN	denotes	of
T2688	9614-9619	WDT	denotes	which
T2689	9620-9623	VBP	denotes	are
T2686	9624-9632	VBN	denotes	involved
T2690	9633-9635	IN	denotes	in
T2691	9636-9649	JJ	denotes	intracellular
T2692	9650-9661	NN	denotes	trafficking
T2693	9662-9664	IN	denotes	of
T2694	9665-9673	NN	denotes	proteins
T2695	9674-9677	CC	denotes	and
T2696	9678-9688	NNS	denotes	organelles
T2697	9689-9691	IN	denotes	in
T2698	9692-9699	NNS	denotes	neurons
T2699	9700-9701	-LRB-	denotes	[
T2701	9701-9703	CD	denotes	36
T2702	9703-9704	,	denotes	,
T2700	9704-9706	CD	denotes	37
T2703	9706-9707	-RRB-	denotes	]
T2704	9707-9708	.	denotes	.
T2705	9708-9861	sentence	denotes	TRIM11 is a member of the tripartite motif protein family and contains a RING finger, a B-box zinc finger, a coiled coil domain and a B30.2 domain [38].
T2706	9709-9715	NN	denotes	TRIM11
T2707	9716-9718	VBZ	denotes	is
T2708	9719-9720	DT	denotes	a
T2709	9721-9727	NN	denotes	member
T2710	9728-9730	IN	denotes	of
T2711	9731-9734	DT	denotes	the
T2713	9735-9745	JJ	denotes	tripartite
T2714	9746-9751	NN	denotes	motif
T2715	9752-9759	NN	denotes	protein
T2712	9760-9766	NN	denotes	family
T2716	9767-9770	CC	denotes	and
T2717	9771-9779	VBZ	denotes	contains
T2718	9780-9781	DT	denotes	a
T2720	9782-9786	NN	denotes	RING
T2719	9787-9793	NN	denotes	finger
T2721	9793-9795	,	denotes	,
T2722	9795-9796	DT	denotes	a
T2724	9797-9798	NN	denotes	B
T2726	9798-9799	HYPH	denotes	-
T2725	9799-9802	NN	denotes	box
T2727	9803-9807	NN	denotes	zinc
T2723	9808-9814	NN	denotes	finger
T2728	9814-9816	,	denotes	,
T2729	9816-9817	DT	denotes	a
T2731	9818-9824	VBN	denotes	coiled
T2732	9825-9829	NN	denotes	coil
T2730	9830-9836	NN	denotes	domain
T2733	9837-9840	CC	denotes	and
T2734	9841-9842	DT	denotes	a
T2736	9843-9848	NN	denotes	B30.2
T2735	9849-9855	NN	denotes	domain
T2737	9856-9857	-LRB-	denotes	[
T2738	9857-9859	CD	denotes	38
T2739	9859-9860	-RRB-	denotes	]
T2740	9860-9861	.	denotes	.
T2741	9861-9959	sentence	denotes	The possible significance of the interactions between these proteins and PAX6 is discussed below.
T2742	9862-9865	DT	denotes	The
T2744	9866-9874	JJ	denotes	possible
T2743	9875-9887	NN	denotes	significance
T2746	9888-9890	IN	denotes	of
T2747	9891-9894	DT	denotes	the
T2748	9895-9907	NNS	denotes	interactions
T2749	9908-9915	IN	denotes	between
T2750	9916-9921	DT	denotes	these
T2751	9922-9930	NN	denotes	proteins
T2752	9931-9934	CC	denotes	and
T2753	9935-9939	NN	denotes	PAX6
T2754	9940-9942	VBZ	denotes	is
T2745	9943-9952	VBN	denotes	discussed
T2755	9953-9958	RB	denotes	below
T2756	9958-9959	.	denotes	.
T2839	9961-9978	JJ	denotes	Semi-quantitative
T2840	9979-9982	NN	denotes	PCR
T2841	9982-10167	sentence	denotes	To check that the Homer3, Dncl1 and Trim11 clones were not identified multiple times solely because they are highly abundant in the library, we performed a semi-quantitative PCR assay.
T2842	9983-9985	TO	denotes	To
T2843	9986-9991	VB	denotes	check
T2845	9992-9996	IN	denotes	that
T2847	9997-10000	DT	denotes	the
T2849	10001-10007	NN	denotes	Homer3
T2850	10007-10009	,	denotes	,
T2851	10009-10014	NN	denotes	Dncl1
T2852	10015-10018	CC	denotes	and
T2853	10019-10025	NN	denotes	Trim11
T2848	10026-10032	NNS	denotes	clones
T2854	10033-10037	VBD	denotes	were
T2855	10038-10041	RB	denotes	not
T2846	10042-10052	VBN	denotes	identified
T2856	10053-10061	JJ	denotes	multiple
T2857	10062-10067	NNS	denotes	times
T2858	10068-10074	RB	denotes	solely
T2860	10075-10082	IN	denotes	because
T2861	10083-10087	PRP	denotes	they
T2859	10088-10091	VBP	denotes	are
T2862	10092-10098	RB	denotes	highly
T2863	10099-10107	JJ	denotes	abundant
T2864	10108-10110	IN	denotes	in
T2865	10111-10114	DT	denotes	the
T2866	10115-10122	NN	denotes	library
T2867	10122-10124	,	denotes	,
T2868	10124-10126	PRP	denotes	we
T2844	10127-10136	VBD	denotes	performed
T2869	10137-10138	DT	denotes	a
T2871	10139-10156	JJ	denotes	semi-quantitative
T2872	10157-10160	NN	denotes	PCR
T2870	10161-10166	NN	denotes	assay
T2873	10166-10167	.	denotes	.
T2874	10167-10259	sentence	denotes	We compared the relative abundance of Homer3, Dncl1, Trim11 and Pax6 with Gapdh and Atp5a1.
T2875	10168-10170	PRP	denotes	We
T2876	10171-10179	VBD	denotes	compared
T2877	10180-10183	DT	denotes	the
T2879	10184-10192	JJ	denotes	relative
T2878	10193-10202	NN	denotes	abundance
T2880	10203-10205	IN	denotes	of
T2881	10206-10212	NN	denotes	Homer3
T2882	10212-10214	,	denotes	,
T2883	10214-10219	NN	denotes	Dncl1
T2884	10219-10221	,	denotes	,
T2885	10221-10227	NN	denotes	Trim11
T2886	10228-10231	CC	denotes	and
T2887	10232-10236	NN	denotes	Pax6
T2888	10237-10241	IN	denotes	with
T2889	10242-10247	NN	denotes	Gapdh
T2890	10248-10251	CC	denotes	and
T2891	10252-10258	NN	denotes	Atp5a1
T2892	10258-10259	.	denotes	.
T2893	10259-10370	sentence	denotes	Gapdh and Atp5a1 both show strong constitutive expression in a variety of tissues including the brain [29,30].
T2894	10260-10265	NN	denotes	Gapdh
T2896	10266-10269	CC	denotes	and
T2897	10270-10276	NN	denotes	Atp5a1
T2898	10277-10281	DT	denotes	both
T2895	10282-10286	VBP	denotes	show
T2899	10287-10293	JJ	denotes	strong
T2901	10294-10306	JJ	denotes	constitutive
T2900	10307-10317	NN	denotes	expression
T2902	10318-10320	IN	denotes	in
T2903	10321-10322	DT	denotes	a
T2904	10323-10330	NN	denotes	variety
T2905	10331-10333	IN	denotes	of
T2906	10334-10341	NNS	denotes	tissues
T2907	10342-10351	VBG	denotes	including
T2908	10352-10355	DT	denotes	the
T2909	10356-10361	NN	denotes	brain
T2910	10362-10363	-LRB-	denotes	[
T2912	10363-10365	CD	denotes	29
T2913	10365-10366	,	denotes	,
T2911	10366-10368	CD	denotes	30
T2914	10368-10369	-RRB-	denotes	]
T2915	10369-10370	.	denotes	.
T2916	10370-10630	sentence	denotes	Homer3, Dncl1, Trim11 and Pax6 were only amplified strongly after 35 cycles of PCR (Figure 2) and were therefore present at relatively low levels compared to Gapdh (amplified strongly after 25 cycles) and Atp5a1 (amplified strongly after 30 cycles; Figure 2).
T2917	10371-10377	NN	denotes	Homer3
T2919	10377-10379	,	denotes	,
T2920	10379-10384	NN	denotes	Dncl1
T2921	10384-10386	,	denotes	,
T2922	10386-10392	NN	denotes	Trim11
T2923	10393-10396	CC	denotes	and
T2924	10397-10401	NN	denotes	Pax6
T2925	10402-10406	VBD	denotes	were
T2926	10407-10411	RB	denotes	only
T2918	10412-10421	VBN	denotes	amplified
T2927	10422-10430	RB	denotes	strongly
T2928	10431-10436	IN	denotes	after
T2929	10437-10439	CD	denotes	35
T2930	10440-10446	NNS	denotes	cycles
T2931	10447-10449	IN	denotes	of
T2932	10450-10453	NN	denotes	PCR
T2933	10454-10455	-LRB-	denotes	(
T2934	10455-10461	NN	denotes	Figure
T2935	10462-10463	CD	denotes	2
T2936	10463-10464	-RRB-	denotes	)
T2937	10465-10468	CC	denotes	and
T2938	10469-10473	VBD	denotes	were
T2939	10474-10483	RB	denotes	therefore
T2940	10484-10491	JJ	denotes	present
T2941	10492-10494	IN	denotes	at
T2942	10495-10505	RB	denotes	relatively
T2943	10506-10509	JJ	denotes	low
T2944	10510-10516	NNS	denotes	levels
T2945	10517-10525	VBN	denotes	compared
T2946	10526-10528	IN	denotes	to
T2947	10529-10534	NN	denotes	Gapdh
T2948	10535-10536	-LRB-	denotes	(
T2949	10536-10545	VBN	denotes	amplified
T2950	10546-10554	RB	denotes	strongly
T2951	10555-10560	IN	denotes	after
T2952	10561-10563	CD	denotes	25
T2953	10564-10570	NNS	denotes	cycles
T2954	10570-10571	-RRB-	denotes	)
T2955	10572-10575	CC	denotes	and
T2956	10576-10582	NN	denotes	Atp5a1
T2957	10583-10584	-LRB-	denotes	(
T2958	10584-10593	VBN	denotes	amplified
T2959	10594-10602	RB	denotes	strongly
T2960	10603-10608	IN	denotes	after
T2961	10609-10611	CD	denotes	30
T2962	10612-10618	NNS	denotes	cycles
T2963	10618-10619	:	denotes	;
T2964	10620-10626	NN	denotes	Figure
T2965	10627-10628	CD	denotes	2
T2966	10628-10629	-RRB-	denotes	)
T2967	10629-10630	.	denotes	.
T2968	10630-11202	sentence	denotes	Figure 2 Semi-quantitative PCR analysis of Homer3, Dncl1, Trim11 and Pax6 in the mouse brain cDNA library. Library cDNA was amplified with primers specific for Pax6 (600 bp), Homer3 (485 bp band), Dncl1 (485 bp) and Trim11 (609 bp) for 20, 25, 30 or 35 cycles. Atp5a1 (415 bp) and Gapdh (450 bp), which are highly expressed in the brain, are included for comparison. M indicates the Φ×174 HaeIII DNA size marker; the positions of the 603 bp and 310 bp marker bands are indicated. We concluded that Homer3, Dncl1 and Trim11 clones were not highly abundant in the library.
T8468	10641-10658	JJ	denotes	Semi-quantitative
T8470	10659-10662	NN	denotes	PCR
T8469	10663-10671	NN	denotes	analysis
T8471	10672-10674	IN	denotes	of
T8472	10675-10681	NN	denotes	Homer3
T8473	10681-10683	,	denotes	,
T8474	10683-10688	NN	denotes	Dncl1
T8475	10688-10690	,	denotes	,
T8476	10690-10696	NN	denotes	Trim11
T8477	10697-10700	CC	denotes	and
T8478	10701-10705	NN	denotes	Pax6
T8479	10706-10708	IN	denotes	in
T8480	10709-10712	DT	denotes	the
T8482	10713-10718	NN	denotes	mouse
T8483	10719-10724	NN	denotes	brain
T8484	10725-10729	NN	denotes	cDNA
T8481	10730-10737	NN	denotes	library
T8485	10737-10738	.	denotes	.
T8486	10738-10892	sentence	denotes	Library cDNA was amplified with primers specific for Pax6 (600 bp), Homer3 (485 bp band), Dncl1 (485 bp) and Trim11 (609 bp) for 20, 25, 30 or 35 cycles.
T8487	10739-10746	NN	denotes	Library
T8488	10747-10751	NN	denotes	cDNA
T8490	10752-10755	VBD	denotes	was
T8489	10756-10765	VBN	denotes	amplified
T8491	10766-10770	IN	denotes	with
T8492	10771-10778	NNS	denotes	primers
T8493	10779-10787	JJ	denotes	specific
T8494	10788-10791	IN	denotes	for
T8495	10792-10796	NN	denotes	Pax6
T8496	10797-10798	-LRB-	denotes	(
T8498	10798-10801	CD	denotes	600
T8497	10802-10804	NN	denotes	bp
T8499	10804-10805	-RRB-	denotes	)
T8500	10805-10807	,	denotes	,
T8501	10807-10813	NN	denotes	Homer3
T8502	10814-10815	-LRB-	denotes	(
T8504	10815-10818	CD	denotes	485
T8505	10819-10821	NN	denotes	bp
T8503	10822-10826	NN	denotes	band
T8506	10826-10827	-RRB-	denotes	)
T8507	10827-10829	,	denotes	,
T8508	10829-10834	NN	denotes	Dncl1
T8509	10835-10836	-LRB-	denotes	(
T8511	10836-10839	CD	denotes	485
T8510	10840-10842	NN	denotes	bp
T8512	10842-10843	-RRB-	denotes	)
T8513	10844-10847	CC	denotes	and
T8514	10848-10854	NN	denotes	Trim11
T8515	10855-10856	-LRB-	denotes	(
T8517	10856-10859	CD	denotes	609
T8516	10860-10862	NN	denotes	bp
T8518	10862-10863	-RRB-	denotes	)
T8519	10864-10867	IN	denotes	for
T8520	10868-10870	CD	denotes	20
T8522	10870-10872	,	denotes	,
T8523	10872-10874	CD	denotes	25
T8524	10874-10876	,	denotes	,
T8525	10876-10878	CD	denotes	30
T8526	10879-10881	CC	denotes	or
T8527	10882-10884	CD	denotes	35
T8521	10885-10891	NNS	denotes	cycles
T8528	10891-10892	.	denotes	.
T8529	10892-10998	sentence	denotes	Atp5a1 (415 bp) and Gapdh (450 bp), which are highly expressed in the brain, are included for comparison.
T8530	10893-10899	NN	denotes	Atp5a1
T8532	10900-10901	-LRB-	denotes	(
T8534	10901-10904	CD	denotes	415
T8533	10905-10907	NN	denotes	bp
T8535	10907-10908	-RRB-	denotes	)
T8536	10909-10912	CC	denotes	and
T8537	10913-10918	NN	denotes	Gapdh
T8538	10919-10920	-LRB-	denotes	(
T8540	10920-10923	CD	denotes	450
T8539	10924-10926	NN	denotes	bp
T8541	10926-10927	-RRB-	denotes	)
T8542	10927-10929	,	denotes	,
T8543	10929-10934	WDT	denotes	which
T8545	10935-10938	VBP	denotes	are
T8546	10939-10945	RB	denotes	highly
T8544	10946-10955	VBN	denotes	expressed
T8547	10956-10958	IN	denotes	in
T8548	10959-10962	DT	denotes	the
T8549	10963-10968	NN	denotes	brain
T8550	10968-10970	,	denotes	,
T8551	10970-10973	VBP	denotes	are
T8531	10974-10982	VBN	denotes	included
T8552	10983-10986	IN	denotes	for
T8553	10987-10997	NN	denotes	comparison
T8554	10997-10998	.	denotes	.
T8555	10998-11111	sentence	denotes	M indicates the Φ×174 HaeIII DNA size marker; the positions of the 603 bp and 310 bp marker bands are indicated.
T8556	10999-11000	NN	denotes	M
T8557	11001-11010	VBZ	denotes	indicates
T8559	11011-11014	DT	denotes	the
T8561	11015-11017	NN	denotes	Φ×
T8563	11017-11020	CD	denotes	174
T8562	11021-11027	NN	denotes	HaeIII
T8564	11028-11031	NN	denotes	DNA
T8565	11032-11036	NN	denotes	size
T8560	11037-11043	NN	denotes	marker
T8566	11043-11044	:	denotes	;
T8567	11045-11048	DT	denotes	the
T8568	11049-11058	NNS	denotes	positions
T8569	11059-11061	IN	denotes	of
T8570	11062-11065	DT	denotes	the
T8572	11066-11069	CD	denotes	603
T8573	11070-11072	NN	denotes	bp
T8574	11073-11076	CC	denotes	and
T8575	11077-11080	CD	denotes	310
T8576	11081-11083	NN	denotes	bp
T8577	11084-11090	NN	denotes	marker
T8571	11091-11096	NNS	denotes	bands
T8578	11097-11100	VBP	denotes	are
T8558	11101-11110	VBN	denotes	indicated
T8579	11110-11111	.	denotes	.
T2969	11112-11114	PRP	denotes	We
T2970	11115-11124	VBD	denotes	concluded
T2971	11125-11129	IN	denotes	that
T2973	11130-11136	NN	denotes	Homer3
T2975	11136-11138	,	denotes	,
T2976	11138-11143	NN	denotes	Dncl1
T2977	11144-11147	CC	denotes	and
T2978	11148-11154	NN	denotes	Trim11
T2974	11155-11161	NNS	denotes	clones
T2972	11162-11166	VBD	denotes	were
T2979	11167-11170	RB	denotes	not
T2980	11171-11177	RB	denotes	highly
T2981	11178-11186	JJ	denotes	abundant
T2982	11187-11189	IN	denotes	in
T2983	11190-11193	DT	denotes	the
T2984	11194-11201	NN	denotes	library
T2985	11201-11202	.	denotes	.
T2986	11202-11363	sentence	denotes	This is consistent with the idea that they were pulled out because the encoded proteins interact specifically with the C-terminal peptide or PST domain of PAX6.
T2987	11203-11207	DT	denotes	This
T2988	11208-11210	VBZ	denotes	is
T2989	11211-11221	JJ	denotes	consistent
T2990	11222-11226	IN	denotes	with
T2991	11227-11230	DT	denotes	the
T2992	11231-11235	NN	denotes	idea
T2993	11236-11240	IN	denotes	that
T2995	11241-11245	PRP	denotes	they
T2996	11246-11250	VBD	denotes	were
T2994	11251-11257	VBN	denotes	pulled
T2997	11258-11261	RP	denotes	out
T2998	11262-11269	IN	denotes	because
T3000	11270-11273	DT	denotes	the
T3002	11274-11281	VBN	denotes	encoded
T3001	11282-11290	NN	denotes	proteins
T2999	11291-11299	VBP	denotes	interact
T3003	11300-11312	RB	denotes	specifically
T3004	11313-11317	IN	denotes	with
T3005	11318-11321	DT	denotes	the
T3007	11322-11323	NN	denotes	C
T3009	11323-11324	HYPH	denotes	-
T3008	11324-11332	JJ	denotes	terminal
T3006	11333-11340	NN	denotes	peptide
T3010	11341-11343	CC	denotes	or
T3011	11344-11347	NN	denotes	PST
T3012	11348-11354	NN	denotes	domain
T3013	11355-11357	IN	denotes	of
T3014	11358-11362	NN	denotes	PAX6
T3015	11362-11363	.	denotes	.
T3382	11365-11370	NN	denotes	Yeast
T3384	11371-11374	CD	denotes	two
T3386	11374-11375	HYPH	denotes	-
T3385	11375-11381	NN	denotes	hybrid
T3387	11382-11390	JJ	denotes	pairwise
T3383	11391-11403	NNS	denotes	interactions
T3388	11403-11599	sentence	denotes	By library screening we identified two proteins (HOMER3 and DNCL1) that interact with the C-terminal peptide and three proteins (HOMER3, DNCL1 and TRIM11) that interact with the whole PST domain.
T3389	11404-11406	IN	denotes	By
T3391	11407-11414	NN	denotes	library
T3392	11415-11424	NN	denotes	screening
T3393	11425-11427	PRP	denotes	we
T3390	11428-11438	VBD	denotes	identified
T3394	11439-11442	CD	denotes	two
T3395	11443-11451	NN	denotes	proteins
T3396	11452-11453	-LRB-	denotes	(
T3397	11453-11459	NN	denotes	HOMER3
T3398	11460-11463	CC	denotes	and
T3399	11464-11469	NN	denotes	DNCL1
T3400	11469-11470	-RRB-	denotes	)
T3401	11471-11475	WDT	denotes	that
T3402	11476-11484	VBP	denotes	interact
T3403	11485-11489	IN	denotes	with
T3404	11490-11493	DT	denotes	the
T3406	11494-11495	NN	denotes	C
T3408	11495-11496	HYPH	denotes	-
T3407	11496-11504	JJ	denotes	terminal
T3405	11505-11512	NN	denotes	peptide
T3409	11513-11516	CC	denotes	and
T3410	11517-11522	CD	denotes	three
T3411	11523-11531	NN	denotes	proteins
T3412	11532-11533	-LRB-	denotes	(
T3413	11533-11539	NN	denotes	HOMER3
T3414	11539-11541	,	denotes	,
T3415	11541-11546	NN	denotes	DNCL1
T3416	11547-11550	CC	denotes	and
T3417	11551-11557	NN	denotes	TRIM11
T3418	11557-11558	-RRB-	denotes	)
T3419	11559-11563	WDT	denotes	that
T3420	11564-11572	VBP	denotes	interact
T3421	11573-11577	IN	denotes	with
T3422	11578-11581	DT	denotes	the
T3424	11582-11587	JJ	denotes	whole
T3425	11588-11591	NN	denotes	PST
T3423	11592-11598	NN	denotes	domain
T3426	11598-11599	.	denotes	.
T3427	11599-11755	sentence	denotes	This suggests that HOMER3 and DNCL1 interact specifically with the C-terminal peptide while TRIM11 interacts with a more N-terminal part of the PST domain.
T3428	11600-11604	DT	denotes	This
T3429	11605-11613	VBZ	denotes	suggests
T3430	11614-11618	IN	denotes	that
T3432	11619-11625	NN	denotes	HOMER3
T3433	11626-11629	CC	denotes	and
T3434	11630-11635	NN	denotes	DNCL1
T3431	11636-11644	VBP	denotes	interact
T3435	11645-11657	RB	denotes	specifically
T3436	11658-11662	IN	denotes	with
T3437	11663-11666	DT	denotes	the
T3439	11667-11668	NN	denotes	C
T3441	11668-11669	HYPH	denotes	-
T3440	11669-11677	JJ	denotes	terminal
T3438	11678-11685	NN	denotes	peptide
T3442	11686-11691	IN	denotes	while
T3444	11692-11698	NN	denotes	TRIM11
T3443	11699-11708	VBZ	denotes	interacts
T3445	11709-11713	IN	denotes	with
T3446	11714-11715	DT	denotes	a
T3448	11716-11720	RBR	denotes	more
T3450	11721-11722	NN	denotes	N
T3451	11722-11723	HYPH	denotes	-
T3449	11723-11731	JJ	denotes	terminal
T3447	11732-11736	NN	denotes	part
T3452	11737-11739	IN	denotes	of
T3453	11740-11743	DT	denotes	the
T3455	11744-11747	NN	denotes	PST
T3454	11748-11754	NN	denotes	domain
T3456	11754-11755	.	denotes	.
T3457	11755-11958	sentence	denotes	We conducted pairwise tests between specific constructs to confirm the interactions identified in the library screen and to further investigate the interaction between PAX6 and HOMER3, DNCL1 and TRIM11.
T3458	11756-11758	PRP	denotes	We
T3459	11759-11768	VBD	denotes	conducted
T3460	11769-11777	JJ	denotes	pairwise
T3461	11778-11783	NNS	denotes	tests
T3462	11784-11791	IN	denotes	between
T3463	11792-11800	JJ	denotes	specific
T3464	11801-11811	NNS	denotes	constructs
T3465	11812-11814	TO	denotes	to
T3466	11815-11822	VB	denotes	confirm
T3467	11823-11826	DT	denotes	the
T3468	11827-11839	NNS	denotes	interactions
T3469	11840-11850	VBN	denotes	identified
T3470	11851-11853	IN	denotes	in
T3471	11854-11857	DT	denotes	the
T3473	11858-11865	NN	denotes	library
T3472	11866-11872	NN	denotes	screen
T3474	11873-11876	CC	denotes	and
T3475	11877-11879	TO	denotes	to
T3477	11880-11887	RB	denotes	further
T3476	11888-11899	VB	denotes	investigate
T3478	11900-11903	DT	denotes	the
T3479	11904-11915	NN	denotes	interaction
T3480	11916-11923	IN	denotes	between
T3481	11924-11928	NN	denotes	PAX6
T3482	11929-11932	CC	denotes	and
T3483	11933-11939	NN	denotes	HOMER3
T3484	11939-11941	,	denotes	,
T3485	11941-11946	NN	denotes	DNCL1
T3486	11947-11950	CC	denotes	and
T3487	11951-11957	NN	denotes	TRIM11
T3488	11957-11958	.	denotes	.
T3489	11958-12099	sentence	denotes	The Dncl1 and Trim11 clones that were pulled out of the library were full-length, but the Homer3 cDNAs lacked the N-terminal 70 amino acids.
T3490	11959-11962	DT	denotes	The
T3492	11963-11968	NN	denotes	Dncl1
T3493	11969-11972	CC	denotes	and
T3494	11973-11979	NN	denotes	Trim11
T3491	11980-11986	NNS	denotes	clones
T3496	11987-11991	WDT	denotes	that
T3498	11992-11996	VBD	denotes	were
T3497	11997-12003	VBN	denotes	pulled
T3499	12004-12007	IN	denotes	out
T3500	12008-12010	IN	denotes	of
T3501	12011-12014	DT	denotes	the
T3502	12015-12022	NN	denotes	library
T3495	12023-12027	VBD	denotes	were
T3503	12028-12032	JJ	denotes	full
T3505	12032-12033	HYPH	denotes	-
T3504	12033-12039	NN	denotes	length
T3506	12039-12041	,	denotes	,
T3507	12041-12044	CC	denotes	but
T3508	12045-12048	DT	denotes	the
T3510	12049-12055	NN	denotes	Homer3
T3509	12056-12061	NNS	denotes	cDNAs
T3511	12062-12068	VBD	denotes	lacked
T3512	12069-12072	DT	denotes	the
T3514	12073-12074	NN	denotes	N
T3516	12074-12075	HYPH	denotes	-
T3515	12075-12083	JJ	denotes	terminal
T3517	12084-12086	CD	denotes	70
T3518	12087-12092	NN	denotes	amino
T3513	12093-12098	NNS	denotes	acids
T3519	12098-12099	.	denotes	.
T3520	12099-12227	sentence	denotes	The missing coding region was inserted into the truncated Homer3 cDNA to give a full-length expression construct (see Methods).
T3521	12100-12103	DT	denotes	The
T3523	12104-12111	JJ	denotes	missing
T3524	12112-12118	NN	denotes	coding
T3522	12119-12125	NN	denotes	region
T3526	12126-12129	VBD	denotes	was
T3525	12130-12138	VBN	denotes	inserted
T3527	12139-12143	IN	denotes	into
T3528	12144-12147	DT	denotes	the
T3530	12148-12157	VBN	denotes	truncated
T3531	12158-12164	NN	denotes	Homer3
T3529	12165-12169	NN	denotes	cDNA
T3532	12170-12172	TO	denotes	to
T3533	12173-12177	VB	denotes	give
T3534	12178-12179	DT	denotes	a
T3536	12180-12184	JJ	denotes	full
T3538	12184-12185	HYPH	denotes	-
T3537	12185-12191	NN	denotes	length
T3539	12192-12202	NN	denotes	expression
T3535	12203-12212	NN	denotes	construct
T3540	12213-12214	-LRB-	denotes	(
T3541	12214-12217	VB	denotes	see
T3542	12218-12225	NNS	denotes	Methods
T3543	12225-12226	-RRB-	denotes	)
T3544	12226-12227	.	denotes	.
T3545	12227-12321	sentence	denotes	Pairwise interactions were carried out with both the full-length and truncated Homer3 clones.
T3546	12228-12236	JJ	denotes	Pairwise
T3547	12237-12249	NNS	denotes	interactions
T3549	12250-12254	VBD	denotes	were
T3548	12255-12262	VBN	denotes	carried
T3550	12263-12266	RP	denotes	out
T3551	12267-12271	IN	denotes	with
T3552	12272-12276	CC	denotes	both
T3554	12277-12280	DT	denotes	the
T3555	12281-12285	JJ	denotes	full
T3557	12285-12286	HYPH	denotes	-
T3556	12286-12292	NN	denotes	length
T3558	12293-12296	CC	denotes	and
T3559	12297-12306	VBN	denotes	truncated
T3560	12307-12313	NN	denotes	Homer3
T3553	12314-12320	NNS	denotes	clones
T3561	12320-12321	.	denotes	.
T3562	12321-12536	sentence	denotes	We confirmed that the whole PAX6 PST domain interacts with HOMER3 (full-length and truncated constructs), TRIM11 and DNCL1, as all three reporter genes were strongly activated in pairwise tests (Figure 3; Table 1).
T3563	12322-12324	PRP	denotes	We
T3564	12325-12334	VBD	denotes	confirmed
T3565	12335-12339	IN	denotes	that
T3567	12340-12343	DT	denotes	the
T3569	12344-12349	JJ	denotes	whole
T3570	12350-12354	NN	denotes	PAX6
T3571	12355-12358	NN	denotes	PST
T3568	12359-12365	NN	denotes	domain
T3566	12366-12375	VBZ	denotes	interacts
T3572	12376-12380	IN	denotes	with
T3573	12381-12387	NN	denotes	HOMER3
T3574	12388-12389	-LRB-	denotes	(
T3576	12389-12393	JJ	denotes	full
T3578	12393-12394	HYPH	denotes	-
T3577	12394-12400	NN	denotes	length
T3579	12401-12404	CC	denotes	and
T3580	12405-12414	VBN	denotes	truncated
T3575	12415-12425	NNS	denotes	constructs
T3581	12425-12426	-RRB-	denotes	)
T3582	12426-12428	,	denotes	,
T3583	12428-12434	NN	denotes	TRIM11
T3584	12435-12438	CC	denotes	and
T3585	12439-12444	NN	denotes	DNCL1
T3586	12444-12446	,	denotes	,
T3587	12446-12448	IN	denotes	as
T3589	12449-12452	DT	denotes	all
T3591	12453-12458	CD	denotes	three
T3592	12459-12467	NN	denotes	reporter
T3590	12468-12473	NNS	denotes	genes
T3593	12474-12478	VBD	denotes	were
T3594	12479-12487	RB	denotes	strongly
T3588	12488-12497	VBN	denotes	activated
T3595	12498-12500	IN	denotes	in
T3596	12501-12509	JJ	denotes	pairwise
T3597	12510-12515	NNS	denotes	tests
T3598	12516-12517	-LRB-	denotes	(
T3600	12517-12523	NN	denotes	Figure
T3601	12524-12525	CD	denotes	3
T3602	12525-12526	:	denotes	;
T3599	12527-12532	NN	denotes	Table
T3603	12533-12534	CD	denotes	1
T3604	12534-12535	-RRB-	denotes	)
T3605	12535-12536	.	denotes	.
T3606	12536-12669	sentence	denotes	In contrast the interaction between the C-terminal peptide and HOMER3 or DNCL1 could not be confirmed with pairwise tests (Table 1).
T3607	12537-12539	IN	denotes	In
T3609	12540-12548	NN	denotes	contrast
T3610	12549-12552	DT	denotes	the
T3611	12553-12564	NN	denotes	interaction
T3612	12565-12572	IN	denotes	between
T3613	12573-12576	DT	denotes	the
T3615	12577-12578	NN	denotes	C
T3617	12578-12579	HYPH	denotes	-
T3616	12579-12587	JJ	denotes	terminal
T3614	12588-12595	NN	denotes	peptide
T3618	12596-12599	CC	denotes	and
T3619	12600-12606	NN	denotes	HOMER3
T3620	12607-12609	CC	denotes	or
T3621	12610-12615	NN	denotes	DNCL1
T3622	12616-12621	MD	denotes	could
T3623	12622-12625	RB	denotes	not
T3624	12626-12628	VB	denotes	be
T3608	12629-12638	VBN	denotes	confirmed
T3625	12639-12643	IN	denotes	with
T3626	12644-12652	JJ	denotes	pairwise
T3627	12653-12658	NNS	denotes	tests
T3628	12659-12660	-LRB-	denotes	(
T3629	12660-12665	NN	denotes	Table
T3630	12666-12667	CD	denotes	1
T3631	12667-12668	-RRB-	denotes	)
T3632	12668-12669	.	denotes	.
T3633	12669-12875	sentence	denotes	Occasionally, partial suppression of growth on plates containing 5-fluoro-orotic acid was observed, indicating low-level activation of the URA3 reporter; however HIS3 and LacZ activation were not observed.
T3634	12670-12682	RB	denotes	Occasionally
T3636	12682-12684	,	denotes	,
T3637	12684-12691	JJ	denotes	partial
T3638	12692-12703	NN	denotes	suppression
T3639	12704-12706	IN	denotes	of
T3640	12707-12713	NN	denotes	growth
T3641	12714-12716	IN	denotes	on
T3642	12717-12723	NNS	denotes	plates
T3643	12724-12734	VBG	denotes	containing
T3644	12735-12736	CD	denotes	5
T3646	12736-12737	HYPH	denotes	-
T3647	12737-12743	JJ	denotes	fluoro
T3648	12743-12744	HYPH	denotes	-
T3645	12744-12750	JJ	denotes	orotic
T3649	12751-12755	NN	denotes	acid
T3650	12756-12759	VBD	denotes	was
T3635	12760-12768	VBN	denotes	observed
T3652	12768-12770	,	denotes	,
T3653	12770-12780	VBG	denotes	indicating
T3654	12781-12784	JJ	denotes	low
T3656	12784-12785	HYPH	denotes	-
T3655	12785-12790	NN	denotes	level
T3657	12791-12801	NN	denotes	activation
T3658	12802-12804	IN	denotes	of
T3659	12805-12808	DT	denotes	the
T3661	12809-12813	NN	denotes	URA3
T3660	12814-12822	NN	denotes	reporter
T3662	12822-12823	:	denotes	;
T3663	12824-12831	RB	denotes	however
T3664	12832-12836	NN	denotes	HIS3
T3666	12837-12840	CC	denotes	and
T3667	12841-12845	NN	denotes	LacZ
T3665	12846-12856	NN	denotes	activation
T3668	12857-12861	VBD	denotes	were
T3669	12862-12865	RB	denotes	not
T3651	12866-12874	VBN	denotes	observed
T3670	12874-12875	.	denotes	.
T3671	12875-13018	sentence	denotes	The reasons for this are not clear, although it may be that the pairwise tests were of sub-optimal sensitivity compared to the library screen.
T3672	12876-12879	DT	denotes	The
T3673	12880-12887	NNS	denotes	reasons
T3675	12888-12891	IN	denotes	for
T3676	12892-12896	DT	denotes	this
T3674	12897-12900	VBP	denotes	are
T3677	12901-12904	RB	denotes	not
T3678	12905-12910	JJ	denotes	clear
T3679	12910-12912	,	denotes	,
T3680	12912-12920	IN	denotes	although
T3682	12921-12923	PRP	denotes	it
T3683	12924-12927	MD	denotes	may
T3681	12928-12930	VB	denotes	be
T3684	12931-12935	IN	denotes	that
T3686	12936-12939	DT	denotes	the
T3688	12940-12948	JJ	denotes	pairwise
T3687	12949-12954	NNS	denotes	tests
T3685	12955-12959	VBD	denotes	were
T3689	12960-12962	IN	denotes	of
T3690	12963-12974	JJ	denotes	sub-optimal
T3691	12975-12986	NN	denotes	sensitivity
T3692	12987-12995	VBN	denotes	compared
T3693	12996-12998	IN	denotes	to
T3694	12999-13002	DT	denotes	the
T3696	13003-13010	NN	denotes	library
T3695	13011-13017	NN	denotes	screen
T3697	13017-13018	.	denotes	.
T3698	13018-13268	sentence	denotes	However we were able to confirm that the C-terminal peptide is important for the interaction with HOMER3 and DNCL1 because interaction with the PAX6PST-CT construct, which lacks the final 32 amino acids, was completely abolished (Figure 3, Table 1).
T3699	13019-13026	RB	denotes	However
T3701	13027-13029	PRP	denotes	we
T3700	13030-13034	VBD	denotes	were
T3702	13035-13039	JJ	denotes	able
T3703	13040-13042	TO	denotes	to
T3704	13043-13050	VB	denotes	confirm
T3705	13051-13055	IN	denotes	that
T3707	13056-13059	DT	denotes	the
T3709	13060-13061	NN	denotes	C
T3711	13061-13062	HYPH	denotes	-
T3710	13062-13070	JJ	denotes	terminal
T3708	13071-13078	NN	denotes	peptide
T3706	13079-13081	VBZ	denotes	is
T3712	13082-13091	JJ	denotes	important
T3713	13092-13095	IN	denotes	for
T3714	13096-13099	DT	denotes	the
T3715	13100-13111	NN	denotes	interaction
T3716	13112-13116	IN	denotes	with
T3717	13117-13123	NN	denotes	HOMER3
T3718	13124-13127	CC	denotes	and
T3719	13128-13133	NN	denotes	DNCL1
T3720	13134-13141	IN	denotes	because
T3722	13142-13153	NN	denotes	interaction
T3723	13154-13158	IN	denotes	with
T3724	13159-13162	DT	denotes	the
T3726	13163-13170	NN	denotes	PAX6PST
T3728	13170-13171	HYPH	denotes	-
T3727	13171-13173	NN	denotes	CT
T3725	13174-13183	NN	denotes	construct
T3729	13183-13185	,	denotes	,
T3730	13185-13190	WDT	denotes	which
T3731	13191-13196	VBZ	denotes	lacks
T3732	13197-13200	DT	denotes	the
T3734	13201-13206	JJ	denotes	final
T3735	13207-13209	CD	denotes	32
T3736	13210-13215	NN	denotes	amino
T3733	13216-13221	NNS	denotes	acids
T3737	13221-13223	,	denotes	,
T3738	13223-13226	VBD	denotes	was
T3739	13227-13237	RB	denotes	completely
T3721	13238-13247	VBN	denotes	abolished
T3740	13248-13249	-LRB-	denotes	(
T3742	13249-13255	NN	denotes	Figure
T3743	13256-13257	CD	denotes	3
T3744	13257-13259	,	denotes	,
T3741	13259-13264	NN	denotes	Table
T3745	13265-13266	CD	denotes	1
T3746	13266-13267	-RRB-	denotes	)
T3747	13267-13268	.	denotes	.
T3748	13268-13348	sentence	denotes	TRIM11 interacted equally well with PAX6PST and PAX6PST-CT (Figure 3, Table 1).
T3749	13269-13275	NN	denotes	TRIM11
T3750	13276-13286	VBD	denotes	interacted
T3751	13287-13294	RB	denotes	equally
T3752	13295-13299	RB	denotes	well
T3753	13300-13304	IN	denotes	with
T3754	13305-13312	NN	denotes	PAX6PST
T3755	13313-13316	CC	denotes	and
T3756	13317-13324	NN	denotes	PAX6PST
T3758	13324-13325	HYPH	denotes	-
T3757	13325-13327	NN	denotes	CT
T3759	13328-13329	-LRB-	denotes	(
T3761	13329-13335	NN	denotes	Figure
T3762	13336-13337	CD	denotes	3
T3763	13337-13339	,	denotes	,
T3760	13339-13344	NN	denotes	Table
T3764	13345-13346	CD	denotes	1
T3765	13346-13347	-RRB-	denotes	)
T3766	13347-13348	.	denotes	.
T3767	13348-13589	sentence	denotes	This is consistent with the library screens in which TRIM11 was isolated with the PST domain but not with the C-terminal peptide and supports the idea that the C-terminal peptide is not important for the interaction between PAX6 and TRIM11.
T3768	13349-13353	DT	denotes	This
T3769	13354-13356	VBZ	denotes	is
T3770	13357-13367	JJ	denotes	consistent
T3771	13368-13372	IN	denotes	with
T3772	13373-13376	DT	denotes	the
T3774	13377-13384	NN	denotes	library
T3773	13385-13392	NNS	denotes	screens
T3775	13393-13395	IN	denotes	in
T3777	13396-13401	WDT	denotes	which
T3778	13402-13408	NN	denotes	TRIM11
T3779	13409-13412	VBD	denotes	was
T3776	13413-13421	VBN	denotes	isolated
T3780	13422-13426	IN	denotes	with
T3781	13427-13430	DT	denotes	the
T3783	13431-13434	NN	denotes	PST
T3782	13435-13441	NN	denotes	domain
T3784	13442-13445	CC	denotes	but
T3785	13446-13449	RB	denotes	not
T3786	13450-13454	IN	denotes	with
T3787	13455-13458	DT	denotes	the
T3789	13459-13460	NN	denotes	C
T3791	13460-13461	HYPH	denotes	-
T3790	13461-13469	JJ	denotes	terminal
T3788	13470-13477	NN	denotes	peptide
T3792	13478-13481	CC	denotes	and
T3793	13482-13490	VBZ	denotes	supports
T3794	13491-13494	DT	denotes	the
T3795	13495-13499	NN	denotes	idea
T3796	13500-13504	IN	denotes	that
T3798	13505-13508	DT	denotes	the
T3800	13509-13510	NN	denotes	C
T3802	13510-13511	HYPH	denotes	-
T3801	13511-13519	JJ	denotes	terminal
T3799	13520-13527	NN	denotes	peptide
T3797	13528-13530	VBZ	denotes	is
T3803	13531-13534	RB	denotes	not
T3804	13535-13544	JJ	denotes	important
T3805	13545-13548	IN	denotes	for
T3806	13549-13552	DT	denotes	the
T3807	13553-13564	NN	denotes	interaction
T3808	13565-13572	IN	denotes	between
T3809	13573-13577	NN	denotes	PAX6
T3810	13578-13581	CC	denotes	and
T3811	13582-13588	NN	denotes	TRIM11
T3812	13588-13589	.	denotes	.
T3813	13589-13590	sentence	denotes
T8632	13600-13604	NN	denotes	LacZ
T8634	13605-13613	NN	denotes	reporter
T8635	13614-13618	NN	denotes	gene
T8633	13619-13629	NN	denotes	activation
T8636	13630-13632	IN	denotes	in
T8637	13633-13641	JJ	denotes	pairwise
T8638	13642-13647	NNS	denotes	tests
T8639	13647-13648	.	denotes	.
T8640	13648-13749	sentence	denotes	pPC86 constructs are shown across the top, and pDBLeu constructs are shown down the right hand side.
T8641	13649-13654	NN	denotes	pPC86
T8642	13655-13665	NNS	denotes	constructs
T8644	13666-13669	VBP	denotes	are
T8643	13670-13675	VBN	denotes	shown
T8645	13676-13682	IN	denotes	across
T8646	13683-13686	DT	denotes	the
T8647	13687-13690	NN	denotes	top
T8648	13690-13692	,	denotes	,
T8649	13692-13695	CC	denotes	and
T8650	13696-13702	NN	denotes	pDBLeu
T8651	13703-13713	NNS	denotes	constructs
T8653	13714-13717	VBP	denotes	are
T8652	13718-13723	VBN	denotes	shown
T8654	13724-13728	IN	denotes	down
T8655	13729-13732	DT	denotes	the
T8657	13733-13738	JJ	denotes	right
T8658	13739-13743	NN	denotes	hand
T8656	13744-13748	NN	denotes	side
T8659	13748-13749	.	denotes	.
T8660	13749-14010	sentence	denotes	PAX6PST, PAX6 PST domain; PAX6PST/Q422R, PAX6 PST domain with the Q422R mutation; PAX6PST/X423L, PAX6 PST domain with the X423L mutation, PAX6PST/1615del10, PAX6 PST domain with the 1615 del10 mutation; PAX6PST-CT, PAX6 PST domain minus the C-terminal peptide.
T8661	13750-13757	NN	denotes	PAX6PST
T8662	13757-13759	,	denotes	,
T8663	13759-13763	NN	denotes	PAX6
T8665	13764-13767	NN	denotes	PST
T8664	13768-13774	NN	denotes	domain
T8666	13774-13775	:	denotes	;
T8667	13776-13783	NN	denotes	PAX6PST
T8669	13783-13784	HYPH	denotes	/
T8668	13784-13789	NN	denotes	Q422R
T8670	13789-13791	,	denotes	,
T8671	13791-13795	NN	denotes	PAX6
T8673	13796-13799	NN	denotes	PST
T8672	13800-13806	NN	denotes	domain
T8674	13807-13811	IN	denotes	with
T8675	13812-13815	DT	denotes	the
T8677	13816-13821	NN	denotes	Q422R
T8676	13822-13830	NN	denotes	mutation
T8678	13830-13831	:	denotes	;
T8679	13832-13839	NN	denotes	PAX6PST
T8681	13839-13840	HYPH	denotes	/
T8680	13840-13845	NN	denotes	X423L
T8682	13845-13847	,	denotes	,
T8683	13847-13851	NN	denotes	PAX6
T8685	13852-13855	NN	denotes	PST
T8684	13856-13862	NN	denotes	domain
T8686	13863-13867	IN	denotes	with
T8687	13868-13871	DT	denotes	the
T8689	13872-13877	NN	denotes	X423L
T8688	13878-13886	NN	denotes	mutation
T8690	13886-13888	,	denotes	,
T8691	13888-13895	NN	denotes	PAX6PST
T8693	13895-13896	HYPH	denotes	/
T8692	13896-13905	NN	denotes	1615del10
T8694	13905-13907	,	denotes	,
T8695	13907-13911	NN	denotes	PAX6
T8697	13912-13915	NN	denotes	PST
T8696	13916-13922	NN	denotes	domain
T8698	13923-13927	IN	denotes	with
T8699	13928-13931	DT	denotes	the
T8701	13932-13936	CD	denotes	1615
T8702	13937-13942	NN	denotes	del10
T8700	13943-13951	NN	denotes	mutation
T8703	13951-13952	:	denotes	;
T8704	13953-13960	NN	denotes	PAX6PST
T8706	13960-13961	HYPH	denotes	-
T8705	13961-13963	NN	denotes	CT
T8707	13963-13965	,	denotes	,
T8708	13965-13969	NN	denotes	PAX6
T8709	13970-13973	NN	denotes	PST
T8710	13974-13980	NN	denotes	domain
T8711	13981-13986	CC	denotes	minus
T8712	13987-13990	DT	denotes	the
T8714	13991-13992	NN	denotes	C
T8716	13992-13993	HYPH	denotes	-
T8715	13993-14001	JJ	denotes	terminal
T8713	14002-14009	NN	denotes	peptide
T8717	14009-14010	.	denotes	.
T8718	14010-14078	sentence	denotes	'Truncated HOMER3' is HOMER3 lacking the N-terminal 70 amino acids.
T8719	14011-14012	``	denotes	'
T8721	14012-14021	VBN	denotes	Truncated
T8722	14022-14028	NN	denotes	HOMER3
T8723	14028-14029	''	denotes	'
T8720	14030-14032	VBZ	denotes	is
T8724	14033-14039	NN	denotes	HOMER3
T8725	14040-14047	VBG	denotes	lacking
T8726	14048-14051	DT	denotes	the
T8728	14052-14053	NN	denotes	N
T8730	14053-14054	HYPH	denotes	-
T8729	14054-14062	JJ	denotes	terminal
T8731	14063-14065	CD	denotes	70
T8732	14066-14071	NN	denotes	amino
T8727	14072-14077	NNS	denotes	acids
T8733	14077-14078	.	denotes	.
T8734	14078-14132	sentence	denotes	Five control strains are shown for comparison (left).
T8735	14079-14083	CD	denotes	Five
T8737	14084-14091	NN	denotes	control
T8736	14092-14099	NNS	denotes	strains
T8739	14100-14103	VBP	denotes	are
T8738	14104-14109	VBN	denotes	shown
T8740	14110-14113	IN	denotes	for
T8741	14114-14124	NN	denotes	comparison
T8742	14125-14126	-LRB-	denotes	(
T8743	14126-14130	JJ	denotes	left
T8744	14130-14131	-RRB-	denotes	)
T8745	14131-14132	.	denotes	.
T8746	14132-14194	sentence	denotes	These range from non-interactor (A) to strong interactor (E).
T8747	14133-14138	DT	denotes	These
T8748	14139-14144	VBP	denotes	range
T8749	14145-14149	IN	denotes	from
T8750	14150-14164	NN	denotes	non-interactor
T8751	14165-14166	-LRB-	denotes	(
T8752	14166-14167	NN	denotes	A
T8753	14167-14168	-RRB-	denotes	)
T8754	14169-14171	IN	denotes	to
T8755	14172-14178	JJ	denotes	strong
T8756	14179-14189	NN	denotes	interactor
T8757	14190-14191	-LRB-	denotes	(
T8758	14191-14192	NN	denotes	E
T8759	14192-14193	-RRB-	denotes	)
T8760	14193-14194	.	denotes	.
T8837	14204-14212	JJ	denotes	Pairwise
T8839	14213-14224	NN	denotes	interaction
T8838	14225-14230	NNS	denotes	tests
T8840	14231-14238	IN	denotes	between
T8841	14239-14245	JJ	denotes	normal
T8843	14246-14249	CC	denotes	and
T8844	14250-14256	JJ	denotes	mutant
T8845	14257-14261	NN	denotes	PAX6
T8842	14262-14272	NNS	denotes	constructs
T8846	14273-14276	CC	denotes	and
T8847	14277-14283	NN	denotes	HOMER3
T8848	14283-14285	,	denotes	,
T8849	14285-14290	NN	denotes	DNCL1
T8850	14291-14294	CC	denotes	and
T8851	14295-14301	NN	denotes	TRIM11
T8852	14301-14302	SYM	denotes	.
T8853	14302-14476	sentence	denotes	+++: strong interaction; ++: moderate interaction; +: weak interaction; (+) borderline interaction with one or two reporters activated at very low levels; 0: no interaction.
T8854	14303-14306	SYM	denotes	+++
T8855	14306-14308	:	denotes	:
T8856	14308-14314	JJ	denotes	strong
T8857	14315-14326	NN	denotes	interaction
T8858	14326-14327	,	denotes	;
T8859	14328-14330	SYM	denotes	++
T8860	14330-14332	:	denotes	:
T8861	14332-14340	JJ	denotes	moderate
T8862	14341-14352	NN	denotes	interaction
T8863	14352-14353	,	denotes	;
T8864	14354-14355	SYM	denotes	+
T8865	14355-14357	:	denotes	:
T8866	14357-14361	JJ	denotes	weak
T8867	14362-14373	NN	denotes	interaction
T8868	14373-14374	,	denotes	;
T8869	14375-14376	-LRB-	denotes	(
T8871	14376-14377	SYM	denotes	+
T8870	14377-14378	-RRB-	denotes	)
T8872	14379-14389	JJ	denotes	borderline
T8873	14390-14401	NN	denotes	interaction
T8874	14402-14406	IN	denotes	with
T8875	14407-14410	CD	denotes	one
T8877	14411-14413	CC	denotes	or
T8878	14414-14417	CD	denotes	two
T8876	14418-14427	NNS	denotes	reporters
T8879	14428-14437	VBN	denotes	activated
T8880	14438-14440	IN	denotes	at
T8881	14441-14445	RB	denotes	very
T8882	14446-14449	JJ	denotes	low
T8883	14450-14456	NNS	denotes	levels
T8884	14456-14457	,	denotes	;
T8885	14458-14459	CD	denotes	0
T8886	14459-14461	:	denotes	:
T8887	14461-14463	DT	denotes	no
T8888	14464-14475	NN	denotes	interaction
T8889	14475-14476	.	denotes	.
T8890	14476-14769	sentence	denotes	PAX6PST, PAX6 PST domain; PAX6CTP, PAX6 C-terminal peptide; PAX6PST-CT, PAX6 PST domain minus the C-terminal peptide; PAX6PST/Q422R, PAX6 PST domain with the Q422R mutation; PAX6PST/X423L, PAX6 PST domain with the X423L mutation, PAX6PST/1615del, PAX6 PST domain with the 1615 del10 mutation.
T8891	14477-14484	NN	denotes	PAX6PST
T8892	14484-14486	,	denotes	,
T8893	14486-14490	NN	denotes	PAX6
T8895	14491-14494	NN	denotes	PST
T8894	14495-14501	NN	denotes	domain
T8896	14501-14502	,	denotes	;
T8897	14503-14510	NN	denotes	PAX6CTP
T8898	14510-14512	,	denotes	,
T8899	14512-14516	NN	denotes	PAX6
T8901	14517-14518	NN	denotes	C
T8903	14518-14519	HYPH	denotes	-
T8902	14519-14527	JJ	denotes	terminal
T8900	14528-14535	NN	denotes	peptide
T8904	14535-14536	,	denotes	;
T8905	14537-14544	NN	denotes	PAX6PST
T8907	14544-14545	HYPH	denotes	-
T8906	14545-14547	NN	denotes	CT
T8908	14547-14549	,	denotes	,
T8909	14549-14553	NN	denotes	PAX6
T8911	14554-14557	NN	denotes	PST
T8910	14558-14564	NN	denotes	domain
T8912	14565-14570	CC	denotes	minus
T8913	14571-14574	DT	denotes	the
T8915	14575-14576	NN	denotes	C
T8917	14576-14577	HYPH	denotes	-
T8916	14577-14585	JJ	denotes	terminal
T8914	14586-14593	NN	denotes	peptide
T8918	14593-14594	,	denotes	;
T8919	14595-14602	NN	denotes	PAX6PST
T8921	14602-14603	HYPH	denotes	/
T8920	14603-14608	NN	denotes	Q422R
T8922	14608-14610	,	denotes	,
T8923	14610-14614	NN	denotes	PAX6
T8925	14615-14618	NN	denotes	PST
T8924	14619-14625	NN	denotes	domain
T8926	14626-14630	IN	denotes	with
T8927	14631-14634	DT	denotes	the
T8929	14635-14640	NN	denotes	Q422R
T8928	14641-14649	NN	denotes	mutation
T8930	14649-14650	,	denotes	;
T8931	14651-14658	NN	denotes	PAX6PST
T8933	14658-14659	HYPH	denotes	/
T8932	14659-14664	NN	denotes	X423L
T8934	14664-14666	,	denotes	,
T8935	14666-14670	NN	denotes	PAX6
T8937	14671-14674	NN	denotes	PST
T8936	14675-14681	NN	denotes	domain
T8938	14682-14686	IN	denotes	with
T8939	14687-14690	DT	denotes	the
T8941	14691-14696	NN	denotes	X423L
T8940	14697-14705	NN	denotes	mutation
T8942	14705-14707	,	denotes	,
T8943	14707-14714	NN	denotes	PAX6PST
T8945	14714-14715	HYPH	denotes	/
T8944	14715-14722	NN	denotes	1615del
T8946	14722-14724	,	denotes	,
T8947	14724-14728	NN	denotes	PAX6
T8949	14729-14732	NN	denotes	PST
T8948	14733-14739	NN	denotes	domain
T8950	14740-14744	IN	denotes	with
T8951	14745-14748	DT	denotes	the
T8953	14749-14753	CD	denotes	1615
T8954	14754-14759	NN	denotes	del10
T8952	14760-14768	NN	denotes	mutation
T8955	14768-14769	.	denotes	.
T8956	14769-14879	sentence	denotes	HOMER3-FL, HOMER3 full-length clone; HOMER3-Tr, truncated HOMER3 clone lacking the N-terminal 70 amino acids.
T8957	14770-14776	NN	denotes	HOMER3
T8959	14776-14777	HYPH	denotes	-
T8958	14777-14779	NN	denotes	FL
T8960	14779-14781	,	denotes	,
T8961	14781-14787	NN	denotes	HOMER3
T8963	14788-14792	JJ	denotes	full
T8965	14792-14793	HYPH	denotes	-
T8964	14793-14799	NN	denotes	length
T8962	14800-14805	NN	denotes	clone
T8966	14805-14806	,	denotes	;
T8967	14807-14813	NN	denotes	HOMER3
T8969	14813-14814	HYPH	denotes	-
T8968	14814-14816	NN	denotes	Tr
T8970	14816-14818	,	denotes	,
T8971	14818-14827	VBN	denotes	truncated
T8973	14828-14834	NN	denotes	HOMER3
T8972	14835-14840	NN	denotes	clone
T8974	14841-14848	VBG	denotes	lacking
T8975	14849-14852	DT	denotes	the
T8977	14853-14854	NN	denotes	N
T8979	14854-14855	HYPH	denotes	-
T8978	14855-14863	JJ	denotes	terminal
T8980	14864-14866	CD	denotes	70
T8981	14867-14872	NN	denotes	amino
T8976	14873-14878	NNS	denotes	acids
T8982	14878-14879	.	denotes	.
T3815	15113-15114	NN	denotes	H
T3814	15113-15115	sentence	denotes	Ha
T3816	15114-15115	VBG	denotes	a
T3818	15115-15116	CD	denotes	v
T3817	15115-15118	sentence	denotes	vin
T3819	15116-15118	NNS	denotes	in
T3821	15118-15119	VBP	denotes	g
T3820	15118-15123	sentence	denotes	g con
T3822	15120-15122	NN	denotes	co
T3823	15122-15123	JJ	denotes	n
T3825	15123-15124	JJ	denotes	f
T3824	15123-15127	sentence	denotes	firm
T3826	15124-15125	VBG	denotes	i
T3827	15125-15127	JJ	denotes	rm
T3829	15127-15129	VBN	denotes	ed
T3828	15127-15356	sentence	denotes	ed that HOMER3, DNCL1 and TRIM11 interact with the PAX6 PST domain, we next investigated how the interactions were affected by three C-terminal PAX6 mutations that have been previously described in patients with ocular anomalies.
T3831	15130-15134	IN	denotes	that
T3833	15135-15141	NN	denotes	HOMER3
T3834	15141-15143	,	denotes	,
T3835	15143-15148	NN	denotes	DNCL1
T3836	15149-15152	CC	denotes	and
T3837	15153-15159	NN	denotes	TRIM11
T3832	15160-15168	VBP	denotes	interact
T3838	15169-15173	IN	denotes	with
T3839	15174-15177	DT	denotes	the
T3841	15178-15182	NN	denotes	PAX6
T3842	15183-15186	NN	denotes	PST
T3840	15187-15193	NN	denotes	domain
T3843	15193-15195	,	denotes	,
T3844	15195-15197	PRP	denotes	we
T3845	15198-15202	RB	denotes	next
T3830	15203-15215	VBD	denotes	investigated
T3846	15216-15219	WRB	denotes	how
T3848	15220-15223	DT	denotes	the
T3849	15224-15236	NNS	denotes	interactions
T3850	15237-15241	VBD	denotes	were
T3847	15242-15250	VBN	denotes	affected
T3851	15251-15253	IN	denotes	by
T3852	15254-15259	CD	denotes	three
T3854	15260-15261	NN	denotes	C
T3856	15261-15262	HYPH	denotes	-
T3855	15262-15270	JJ	denotes	terminal
T3857	15271-15275	NN	denotes	PAX6
T3853	15276-15285	NNS	denotes	mutations
T3858	15286-15290	WDT	denotes	that
T3860	15291-15295	VBP	denotes	have
T3861	15296-15300	VBN	denotes	been
T3862	15301-15311	RB	denotes	previously
T3859	15312-15321	VBN	denotes	described
T3863	15322-15324	IN	denotes	in
T3864	15325-15333	NNS	denotes	patients
T3865	15334-15338	IN	denotes	with
T3866	15339-15345	JJ	denotes	ocular
T3867	15346-15355	NNS	denotes	anomalies
T3868	15355-15356	.	denotes	.
T3869	15356-15506	sentence	denotes	The first mutation is a single nucleotide substitution 1627A>G that causes a glutamine to arginine amino acid substitution in the last codon of PAX6.
T3870	15357-15360	DT	denotes	The
T3872	15361-15366	JJ	denotes	first
T3871	15367-15375	NN	denotes	mutation
T3873	15376-15378	VBZ	denotes	is
T3874	15379-15380	DT	denotes	a
T3876	15381-15387	JJ	denotes	single
T3877	15388-15398	NN	denotes	nucleotide
T3875	15399-15411	NN	denotes	substitution
T3878	15412-15417	NN	denotes	1627A
T3879	15417-15418	SYM	denotes	>
T3880	15418-15419	NN	denotes	G
T3881	15420-15424	WDT	denotes	that
T3882	15425-15431	VBZ	denotes	causes
T3883	15432-15433	DT	denotes	a
T3885	15434-15443	NN	denotes	glutamine
T3886	15444-15446	IN	denotes	to
T3887	15447-15455	NN	denotes	arginine
T3888	15456-15461	NN	denotes	amino
T3889	15462-15466	NN	denotes	acid
T3884	15467-15479	NN	denotes	substitution
T3890	15480-15482	IN	denotes	in
T3891	15483-15486	DT	denotes	the
T3893	15487-15491	JJ	denotes	last
T3892	15492-15497	NN	denotes	codon
T3894	15498-15500	IN	denotes	of
T3895	15501-15505	NN	denotes	PAX6
T3896	15505-15506	.	denotes	.
T3897	15506-15698	sentence	denotes	This missense mutation (Q422R) has been reported in two patients, one affected by anterior segment dysgenesis with uveal ectropion and one with typical aniridia and foveal hypoplasia [27,33].
T3898	15507-15511	DT	denotes	This
T3900	15512-15520	JJ	denotes	missense
T3899	15521-15529	NN	denotes	mutation
T3902	15530-15531	-LRB-	denotes	(
T3903	15531-15536	NN	denotes	Q422R
T3904	15536-15537	-RRB-	denotes	)
T3905	15538-15541	VBZ	denotes	has
T3906	15542-15546	VBN	denotes	been
T3901	15547-15555	VBN	denotes	reported
T3907	15556-15558	IN	denotes	in
T3908	15559-15562	CD	denotes	two
T3909	15563-15571	NNS	denotes	patients
T3910	15571-15573	,	denotes	,
T3911	15573-15576	CD	denotes	one
T3912	15577-15585	VBN	denotes	affected
T3913	15586-15588	IN	denotes	by
T3914	15589-15597	JJ	denotes	anterior
T3916	15598-15605	NN	denotes	segment
T3915	15606-15616	NN	denotes	dysgenesis
T3917	15617-15621	IN	denotes	with
T3918	15622-15627	JJ	denotes	uveal
T3919	15628-15637	NN	denotes	ectropion
T3920	15638-15641	CC	denotes	and
T3921	15642-15645	CD	denotes	one
T3922	15646-15650	IN	denotes	with
T3923	15651-15658	JJ	denotes	typical
T3924	15659-15667	NN	denotes	aniridia
T3925	15668-15671	CC	denotes	and
T3926	15672-15678	JJ	denotes	foveal
T3927	15679-15689	NN	denotes	hypoplasia
T3928	15690-15691	-LRB-	denotes	[
T3930	15691-15693	CD	denotes	27
T3931	15693-15694	,	denotes	,
T3929	15694-15696	CD	denotes	33
T3932	15696-15697	-RRB-	denotes	]
T3933	15697-15698	.	denotes	.
T3934	15698-15768	sentence	denotes	The second mutation (1615del10) was found in an aniridia family [28].
T3935	15699-15702	DT	denotes	The
T3937	15703-15709	JJ	denotes	second
T3936	15710-15718	NN	denotes	mutation
T3939	15719-15720	-LRB-	denotes	(
T3940	15720-15729	NN	denotes	1615del10
T3941	15729-15730	-RRB-	denotes	)
T3942	15731-15734	VBD	denotes	was
T3938	15735-15740	VBN	denotes	found
T3943	15741-15743	IN	denotes	in
T3944	15744-15746	DT	denotes	an
T3946	15747-15755	NN	denotes	aniridia
T3945	15756-15762	NN	denotes	family
T3947	15763-15764	-LRB-	denotes	[
T3948	15764-15766	CD	denotes	28
T3949	15766-15767	-RRB-	denotes	]
T3950	15767-15768	.	denotes	.
T3951	15768-16045	sentence	denotes	This frame-shifting deletion occurs just before the PAX6 stop codon and is predicted to cause translational read-through into the 3' untranslated region, generating a protein in which the last 5 amino acids of the C-terminal peptide are replaced by a 103 amino acid-extension.
T3952	15769-15773	DT	denotes	This
T3954	15774-15779	NN	denotes	frame
T3956	15779-15780	HYPH	denotes	-
T3955	15780-15788	VBG	denotes	shifting
T3953	15789-15797	NN	denotes	deletion
T3957	15798-15804	VBZ	denotes	occurs
T3958	15805-15809	RB	denotes	just
T3959	15810-15816	IN	denotes	before
T3960	15817-15820	DT	denotes	the
T3962	15821-15825	NN	denotes	PAX6
T3963	15826-15830	NN	denotes	stop
T3961	15831-15836	NN	denotes	codon
T3964	15837-15840	CC	denotes	and
T3965	15841-15843	VBZ	denotes	is
T3966	15844-15853	VBN	denotes	predicted
T3967	15854-15856	TO	denotes	to
T3968	15857-15862	VB	denotes	cause
T3969	15863-15876	JJ	denotes	translational
T3971	15877-15881	NN	denotes	read
T3972	15881-15882	HYPH	denotes	-
T3970	15882-15889	NN	denotes	through
T3973	15890-15894	IN	denotes	into
T3974	15895-15898	DT	denotes	the
T3976	15899-15900	CD	denotes	3
T3977	15900-15901	SYM	denotes	'
T3978	15902-15914	JJ	denotes	untranslated
T3975	15915-15921	NN	denotes	region
T3979	15921-15923	,	denotes	,
T3980	15923-15933	VBG	denotes	generating
T3981	15934-15935	DT	denotes	a
T3982	15936-15943	NN	denotes	protein
T3983	15944-15946	IN	denotes	in
T3985	15947-15952	WDT	denotes	which
T3986	15953-15956	DT	denotes	the
T3988	15957-15961	JJ	denotes	last
T3989	15962-15963	CD	denotes	5
T3990	15964-15969	NN	denotes	amino
T3987	15970-15975	NNS	denotes	acids
T3991	15976-15978	IN	denotes	of
T3992	15979-15982	DT	denotes	the
T3994	15983-15984	NN	denotes	C
T3996	15984-15985	HYPH	denotes	-
T3995	15985-15993	JJ	denotes	terminal
T3993	15994-16001	NN	denotes	peptide
T3997	16002-16005	VBP	denotes	are
T3984	16006-16014	VBN	denotes	replaced
T3998	16015-16017	IN	denotes	by
T3999	16018-16019	DT	denotes	a
T4001	16020-16023	CD	denotes	103
T4003	16024-16029	NN	denotes	amino
T4002	16030-16034	NN	denotes	acid
T4004	16034-16035	HYPH	denotes	-
T4000	16035-16044	NN	denotes	extension
T4005	16044-16045	.	denotes	.
T4006	16045-16189	sentence	denotes	Affected individuals in this family showed unusual neurobehavioural traits including impaired social cognition and poor verbal inhibition [28].
T4007	16046-16054	VBN	denotes	Affected
T4008	16055-16066	NNS	denotes	individuals
T4010	16067-16069	IN	denotes	in
T4011	16070-16074	DT	denotes	this
T4012	16075-16081	NN	denotes	family
T4009	16082-16088	VBD	denotes	showed
T4013	16089-16096	JJ	denotes	unusual
T4015	16097-16113	JJ	denotes	neurobehavioural
T4014	16114-16120	NNS	denotes	traits
T4016	16121-16130	VBG	denotes	including
T4017	16131-16139	VBN	denotes	impaired
T4019	16140-16146	JJ	denotes	social
T4018	16147-16156	NN	denotes	cognition
T4020	16157-16160	CC	denotes	and
T4021	16161-16165	JJ	denotes	poor
T4023	16166-16172	JJ	denotes	verbal
T4022	16173-16183	NN	denotes	inhibition
T4024	16184-16185	-LRB-	denotes	[
T4025	16185-16187	CD	denotes	28
T4026	16187-16188	-RRB-	denotes	]
T4027	16188-16189	.	denotes	.
T4028	16189-16343	sentence	denotes	MRI analysis revealed grey matter abnormalities in the frontal lobe, temporal lobe and cerebellum, and white matter deficits in the corpus callosum [13].
T4029	16190-16193	NN	denotes	MRI
T4030	16194-16202	NN	denotes	analysis
T4031	16203-16211	VBD	denotes	revealed
T4032	16212-16216	JJ	denotes	grey
T4033	16217-16223	NN	denotes	matter
T4034	16224-16237	NNS	denotes	abnormalities
T4035	16238-16240	IN	denotes	in
T4036	16241-16244	DT	denotes	the
T4038	16245-16252	JJ	denotes	frontal
T4037	16253-16257	NN	denotes	lobe
T4039	16257-16259	,	denotes	,
T4040	16259-16267	JJ	denotes	temporal
T4041	16268-16272	NN	denotes	lobe
T4042	16273-16276	CC	denotes	and
T4043	16277-16287	NN	denotes	cerebellum
T4044	16287-16289	,	denotes	,
T4045	16289-16292	CC	denotes	and
T4046	16293-16298	JJ	denotes	white
T4047	16299-16305	NN	denotes	matter
T4048	16306-16314	NNS	denotes	deficits
T4049	16315-16317	IN	denotes	in
T4050	16318-16321	DT	denotes	the
T4052	16322-16328	NN	denotes	corpus
T4051	16329-16337	NN	denotes	callosum
T4053	16338-16339	-LRB-	denotes	[
T4054	16339-16341	CD	denotes	13
T4055	16341-16342	-RRB-	denotes	]
T4056	16342-16343	.	denotes	.
T4057	16343-16441	sentence	denotes	The third mutation 1629insT (X423L) has been reported in several aniridia patients [11,12,27,33].
T4058	16344-16347	DT	denotes	The
T4060	16348-16353	JJ	denotes	third
T4059	16354-16362	NN	denotes	mutation
T4062	16363-16371	NN	denotes	1629insT
T4063	16372-16373	-LRB-	denotes	(
T4064	16373-16378	NN	denotes	X423L
T4065	16378-16379	-RRB-	denotes	)
T4066	16380-16383	VBZ	denotes	has
T4067	16384-16388	VBN	denotes	been
T4061	16389-16397	VBN	denotes	reported
T4068	16398-16400	IN	denotes	in
T4069	16401-16408	JJ	denotes	several
T4071	16409-16417	NN	denotes	aniridia
T4070	16418-16426	NNS	denotes	patients
T4072	16427-16428	-LRB-	denotes	[
T4074	16428-16430	CD	denotes	11
T4075	16430-16431	,	denotes	,
T4076	16431-16433	CD	denotes	12
T4077	16433-16434	,	denotes	,
T4078	16434-16436	CD	denotes	27
T4079	16436-16437	,	denotes	,
T4073	16437-16439	CD	denotes	33
T4080	16439-16440	-RRB-	denotes	]
T4081	16440-16441	.	denotes	.
T4082	16441-16691	sentence	denotes	Insertion of a single T nucleotide at position 1629 changes the stop codon (TAA) to a leucine codon (TTA) and generates a full length PAX6 protein with a C-terminal extension that extends for a further 35 amino acids into the 3' untranslated region.
T4083	16442-16451	NN	denotes	Insertion
T4085	16452-16454	IN	denotes	of
T4086	16455-16456	DT	denotes	a
T4088	16457-16463	JJ	denotes	single
T4089	16464-16465	NN	denotes	T
T4087	16466-16476	NN	denotes	nucleotide
T4090	16477-16479	IN	denotes	at
T4091	16480-16488	NN	denotes	position
T4092	16489-16493	CD	denotes	1629
T4084	16494-16501	VBZ	denotes	changes
T4093	16502-16505	DT	denotes	the
T4095	16506-16510	NN	denotes	stop
T4094	16511-16516	NN	denotes	codon
T4096	16517-16518	-LRB-	denotes	(
T4097	16518-16521	NN	denotes	TAA
T4098	16521-16522	-RRB-	denotes	)
T4099	16523-16525	IN	denotes	to
T4100	16526-16527	DT	denotes	a
T4102	16528-16535	NN	denotes	leucine
T4101	16536-16541	NN	denotes	codon
T4103	16542-16543	-LRB-	denotes	(
T4104	16543-16546	NN	denotes	TTA
T4105	16546-16547	-RRB-	denotes	)
T4106	16548-16551	CC	denotes	and
T4107	16552-16561	VBZ	denotes	generates
T4108	16562-16563	DT	denotes	a
T4110	16564-16568	JJ	denotes	full
T4111	16569-16575	NN	denotes	length
T4112	16576-16580	NN	denotes	PAX6
T4109	16581-16588	NN	denotes	protein
T4113	16589-16593	IN	denotes	with
T4114	16594-16595	DT	denotes	a
T4116	16596-16597	NN	denotes	C
T4118	16597-16598	HYPH	denotes	-
T4117	16598-16606	JJ	denotes	terminal
T4115	16607-16616	NN	denotes	extension
T4119	16617-16621	WDT	denotes	that
T4120	16622-16629	VBZ	denotes	extends
T4121	16630-16633	IN	denotes	for
T4122	16634-16635	DT	denotes	a
T4124	16636-16643	JJ	denotes	further
T4125	16644-16646	CD	denotes	35
T4126	16647-16652	NN	denotes	amino
T4123	16653-16658	NNS	denotes	acids
T4127	16659-16663	IN	denotes	into
T4128	16664-16667	DT	denotes	the
T4130	16668-16669	CD	denotes	3
T4131	16669-16670	SYM	denotes	'
T4132	16671-16683	JJ	denotes	untranslated
T4129	16684-16690	NN	denotes	region
T4133	16690-16691	.	denotes	.
T4134	16691-16870	sentence	denotes	MRI analysis of six patients with this mutation revealed variable brain defects including absence or hypoplasia of the anterior commissure, pineal gland and olfactory bulbs [12].
T4135	16692-16695	NN	denotes	MRI
T4136	16696-16704	NN	denotes	analysis
T4138	16705-16707	IN	denotes	of
T4139	16708-16711	CD	denotes	six
T4140	16712-16720	NNS	denotes	patients
T4141	16721-16725	IN	denotes	with
T4142	16726-16730	DT	denotes	this
T4143	16731-16739	NN	denotes	mutation
T4137	16740-16748	VBD	denotes	revealed
T4144	16749-16757	JJ	denotes	variable
T4146	16758-16763	NN	denotes	brain
T4145	16764-16771	NNS	denotes	defects
T4147	16772-16781	VBG	denotes	including
T4148	16782-16789	NN	denotes	absence
T4149	16790-16792	CC	denotes	or
T4150	16793-16803	NN	denotes	hypoplasia
T4151	16804-16806	IN	denotes	of
T4152	16807-16810	DT	denotes	the
T4154	16811-16819	JJ	denotes	anterior
T4153	16820-16830	NN	denotes	commissure
T4155	16830-16832	,	denotes	,
T4156	16832-16838	JJ	denotes	pineal
T4157	16839-16844	NN	denotes	gland
T4158	16845-16848	CC	denotes	and
T4159	16849-16858	JJ	denotes	olfactory
T4160	16859-16864	NNS	denotes	bulbs
T4161	16865-16866	-LRB-	denotes	[
T4162	16866-16868	CD	denotes	12
T4163	16868-16869	-RRB-	denotes	]
T4164	16869-16870	.	denotes	.
T4165	16870-16951	sentence	denotes	Two patients had temporal polymicrogyria, one in association with epilepsy [12].
T4166	16871-16874	CD	denotes	Two
T4167	16875-16883	NNS	denotes	patients
T4168	16884-16887	VBD	denotes	had
T4170	16888-16896	JJ	denotes	temporal
T4171	16897-16911	NN	denotes	polymicrogyria
T4172	16911-16913	,	denotes	,
T4169	16913-16916	CD	denotes	one
T4173	16917-16919	IN	denotes	in
T4174	16920-16931	NN	denotes	association
T4175	16932-16936	IN	denotes	with
T4176	16937-16945	NN	denotes	epilepsy
T4177	16946-16947	-LRB-	denotes	[
T4178	16947-16949	CD	denotes	12
T4179	16949-16950	-RRB-	denotes	]
T4180	16950-16951	.	denotes	.
T4181	16951-17136	sentence	denotes	The three mutations were introduced into the PAX6PST construct, and pairwise tests were carried out to investigate the interaction of each mutant protein with HOMER3, DNCL1 and TRIM11.
T4182	16952-16955	DT	denotes	The
T4184	16956-16961	CD	denotes	three
T4183	16962-16971	NNS	denotes	mutations
T4186	16972-16976	VBD	denotes	were
T4185	16977-16987	VBN	denotes	introduced
T4187	16988-16992	IN	denotes	into
T4188	16993-16996	DT	denotes	the
T4190	16997-17004	NN	denotes	PAX6PST
T4189	17005-17014	NN	denotes	construct
T4191	17014-17016	,	denotes	,
T4192	17016-17019	CC	denotes	and
T4193	17020-17028	JJ	denotes	pairwise
T4194	17029-17034	NNS	denotes	tests
T4196	17035-17039	VBD	denotes	were
T4195	17040-17047	VBN	denotes	carried
T4197	17048-17051	RP	denotes	out
T4198	17052-17054	TO	denotes	to
T4199	17055-17066	VB	denotes	investigate
T4200	17067-17070	DT	denotes	the
T4201	17071-17082	NN	denotes	interaction
T4202	17083-17085	IN	denotes	of
T4203	17086-17090	DT	denotes	each
T4205	17091-17097	NN	denotes	mutant
T4204	17098-17105	NN	denotes	protein
T4206	17106-17110	IN	denotes	with
T4207	17111-17117	NN	denotes	HOMER3
T4208	17117-17119	,	denotes	,
T4209	17119-17124	NN	denotes	DNCL1
T4210	17125-17128	CC	denotes	and
T4211	17129-17135	NN	denotes	TRIM11
T4212	17135-17136	.	denotes	.
T4213	17136-17196	sentence	denotes	All three mutations had a clear effect on the interactions.
T4214	17137-17140	DT	denotes	All
T4216	17141-17146	CD	denotes	three
T4215	17147-17156	NNS	denotes	mutations
T4217	17157-17160	VBD	denotes	had
T4218	17161-17162	DT	denotes	a
T4220	17163-17168	JJ	denotes	clear
T4219	17169-17175	NN	denotes	effect
T4221	17176-17178	IN	denotes	on
T4222	17179-17182	DT	denotes	the
T4223	17183-17195	NNS	denotes	interactions
T4224	17195-17196	.	denotes	.
T4225	17196-17310	sentence	denotes	The most subtle mutation (Q422R) caused a reduction in the interaction with HOMER3 and DNCL1 (Figure 3, Table 1).
T4226	17197-17200	DT	denotes	The
T4228	17201-17205	RBS	denotes	most
T4229	17206-17212	JJ	denotes	subtle
T4227	17213-17221	NN	denotes	mutation
T4231	17222-17223	-LRB-	denotes	(
T4232	17223-17228	NN	denotes	Q422R
T4233	17228-17229	-RRB-	denotes	)
T4230	17230-17236	VBD	denotes	caused
T4234	17237-17238	DT	denotes	a
T4235	17239-17248	NN	denotes	reduction
T4236	17249-17251	IN	denotes	in
T4237	17252-17255	DT	denotes	the
T4238	17256-17267	NN	denotes	interaction
T4239	17268-17272	IN	denotes	with
T4240	17273-17279	NN	denotes	HOMER3
T4241	17280-17283	CC	denotes	and
T4242	17284-17289	NN	denotes	DNCL1
T4243	17290-17291	-LRB-	denotes	(
T4245	17291-17297	NN	denotes	Figure
T4246	17298-17299	CD	denotes	3
T4247	17299-17301	,	denotes	,
T4244	17301-17306	NN	denotes	Table
T4248	17307-17308	CD	denotes	1
T4249	17308-17309	-RRB-	denotes	)
T4250	17309-17310	.	denotes	.
T4251	17310-17472	sentence	denotes	The C-terminal extension mutations X423L and1615del10 mutations both dramatically reduced or abolished the interaction with HOMER3 and DNCL1 (Figure 3, Table 1).
T4252	17311-17314	DT	denotes	The
T4254	17315-17316	NN	denotes	C
T4256	17316-17317	HYPH	denotes	-
T4255	17317-17325	JJ	denotes	terminal
T4257	17326-17335	NN	denotes	extension
T4258	17336-17345	NNS	denotes	mutations
T4253	17346-17351	NN	denotes	X423L
T4260	17352-17355	CC	denotes	and
T4261	17355-17364	NN	denotes	1615del10
T4262	17365-17374	NNS	denotes	mutations
T4263	17375-17379	DT	denotes	both
T4264	17380-17392	RB	denotes	dramatically
T4259	17393-17400	VBD	denotes	reduced
T4265	17401-17403	CC	denotes	or
T4266	17404-17413	VBD	denotes	abolished
T4267	17414-17417	DT	denotes	the
T4268	17418-17429	NN	denotes	interaction
T4269	17430-17434	IN	denotes	with
T4270	17435-17441	NN	denotes	HOMER3
T4271	17442-17445	CC	denotes	and
T4272	17446-17451	NN	denotes	DNCL1
T4273	17452-17453	-LRB-	denotes	(
T4275	17453-17459	NN	denotes	Figure
T4276	17460-17461	CD	denotes	3
T4277	17461-17463	,	denotes	,
T4274	17463-17468	NN	denotes	Table
T4278	17469-17470	CD	denotes	1
T4279	17470-17471	-RRB-	denotes	)
T4280	17471-17472	.	denotes	.
T4281	17472-17652	sentence	denotes	None of the three mutations affected the interaction with TRIM11 which again is consistent with the hypothesis that TRIM11 interacts with a more N-terminal part of the PST domain.
T4282	17473-17477	NN	denotes	None
T4284	17478-17480	IN	denotes	of
T4285	17481-17484	DT	denotes	the
T4287	17485-17490	CD	denotes	three
T4286	17491-17500	NNS	denotes	mutations
T4283	17501-17509	VBD	denotes	affected
T4288	17510-17513	DT	denotes	the
T4289	17514-17525	NN	denotes	interaction
T4290	17526-17530	IN	denotes	with
T4291	17531-17537	NN	denotes	TRIM11
T4292	17538-17543	WDT	denotes	which
T4294	17544-17549	RB	denotes	again
T4293	17550-17552	VBZ	denotes	is
T4295	17553-17563	JJ	denotes	consistent
T4296	17564-17568	IN	denotes	with
T4297	17569-17572	DT	denotes	the
T4298	17573-17583	NN	denotes	hypothesis
T4299	17584-17588	IN	denotes	that
T4301	17589-17595	NN	denotes	TRIM11
T4300	17596-17605	VBZ	denotes	interacts
T4302	17606-17610	IN	denotes	with
T4303	17611-17612	DT	denotes	a
T4305	17613-17617	RBR	denotes	more
T4307	17618-17619	NN	denotes	N
T4308	17619-17620	HYPH	denotes	-
T4306	17620-17628	JJ	denotes	terminal
T4304	17629-17633	NN	denotes	part
T4309	17634-17636	IN	denotes	of
T4310	17637-17640	DT	denotes	the
T4312	17641-17644	NN	denotes	PST
T4311	17645-17651	NN	denotes	domain
T4313	17651-17652	.	denotes	.
T5078	17665-17667	IN	denotes	On
T5080	17668-17671	DT	denotes	the
T5081	17672-17677	NN	denotes	basis
T5082	17678-17680	IN	denotes	of
T5083	17681-17690	JJ	denotes	secondary
T5084	17691-17700	NN	denotes	structure
T5085	17701-17712	NNS	denotes	predictions
T5086	17713-17716	CC	denotes	and
T5087	17717-17722	NN	denotes	amino
T5088	17723-17727	NN	denotes	acid
T5090	17728-17736	NN	denotes	sequence
T5089	17737-17749	NN	denotes	conservation
T5091	17749-17751	,	denotes	,
T5092	17751-17753	PRP	denotes	we
T5079	17754-17761	VBD	denotes	defined
T5093	17762-17763	DT	denotes	a
T5095	17764-17769	JJ	denotes	novel
T5096	17770-17774	NN	denotes	PAX6
T5097	17775-17782	NN	denotes	protein
T5094	17783-17789	NN	denotes	domain
T5098	17789-17791	,	denotes	,
T5099	17791-17796	WDT	denotes	which
T5101	17797-17799	PRP	denotes	we
T5102	17800-17804	VBP	denotes	have
T5100	17805-17811	VBN	denotes	called
T5103	17812-17815	DT	denotes	the
T5105	17816-17817	NN	denotes	C
T5107	17817-17818	HYPH	denotes	-
T5106	17818-17826	JJ	denotes	terminal
T5104	17827-17834	NN	denotes	peptide
T5108	17834-17835	.	denotes	.
T5109	17835-18012	sentence	denotes	We performed yeast two-hybrid library screens with the C-terminal peptide and the whole PST domain and we identified three novel interacting proteins, HOMER3, DNCL1 and TRIM11.
T5110	17836-17838	PRP	denotes	We
T5111	17839-17848	VBD	denotes	performed
T5112	17849-17854	NN	denotes	yeast
T5114	17855-17858	CD	denotes	two
T5116	17858-17859	HYPH	denotes	-
T5115	17859-17865	NN	denotes	hybrid
T5117	17866-17873	NN	denotes	library
T5113	17874-17881	NNS	denotes	screens
T5118	17882-17886	IN	denotes	with
T5119	17887-17890	DT	denotes	the
T5121	17891-17892	NN	denotes	C
T5123	17892-17893	HYPH	denotes	-
T5122	17893-17901	JJ	denotes	terminal
T5120	17902-17909	NN	denotes	peptide
T5124	17910-17913	CC	denotes	and
T5125	17914-17917	DT	denotes	the
T5127	17918-17923	JJ	denotes	whole
T5128	17924-17927	NN	denotes	PST
T5126	17928-17934	NN	denotes	domain
T5129	17935-17938	CC	denotes	and
T5130	17939-17941	PRP	denotes	we
T5131	17942-17952	VBD	denotes	identified
T5132	17953-17958	CD	denotes	three
T5134	17959-17964	JJ	denotes	novel
T5135	17965-17976	VBG	denotes	interacting
T5133	17977-17985	NN	denotes	proteins
T5136	17985-17987	,	denotes	,
T5137	17987-17993	NN	denotes	HOMER3
T5138	17993-17995	,	denotes	,
T5139	17995-18000	NN	denotes	DNCL1
T5140	18001-18004	CC	denotes	and
T5141	18005-18011	NN	denotes	TRIM11
T5142	18011-18012	.	denotes	.
T5143	18012-18310	sentence	denotes	In library screens, HOMER3 and DNCL1 interacted with the C-terminal peptide and the PST domain while TRIM11 interacted only with the PST domain, suggesting that HOMER3 and DNCL1 specifically interact with the C-terminal peptide while TRIM11 interacts with a more N-terminal part of the PST domain.
T5144	18013-18015	IN	denotes	In
T5146	18016-18023	NN	denotes	library
T5147	18024-18031	NNS	denotes	screens
T5148	18031-18033	,	denotes	,
T5149	18033-18039	NN	denotes	HOMER3
T5150	18040-18043	CC	denotes	and
T5151	18044-18049	NN	denotes	DNCL1
T5145	18050-18060	VBD	denotes	interacted
T5152	18061-18065	IN	denotes	with
T5153	18066-18069	DT	denotes	the
T5155	18070-18071	NN	denotes	C
T5157	18071-18072	HYPH	denotes	-
T5156	18072-18080	JJ	denotes	terminal
T5154	18081-18088	NN	denotes	peptide
T5158	18089-18092	CC	denotes	and
T5159	18093-18096	DT	denotes	the
T5161	18097-18100	NN	denotes	PST
T5160	18101-18107	NN	denotes	domain
T5162	18108-18113	IN	denotes	while
T5164	18114-18120	NN	denotes	TRIM11
T5163	18121-18131	VBD	denotes	interacted
T5165	18132-18136	RB	denotes	only
T5166	18137-18141	IN	denotes	with
T5167	18142-18145	DT	denotes	the
T5169	18146-18149	NN	denotes	PST
T5168	18150-18156	NN	denotes	domain
T5170	18156-18158	,	denotes	,
T5171	18158-18168	VBG	denotes	suggesting
T5172	18169-18173	IN	denotes	that
T5174	18174-18180	NN	denotes	HOMER3
T5175	18181-18184	CC	denotes	and
T5176	18185-18190	NN	denotes	DNCL1
T5177	18191-18203	RB	denotes	specifically
T5173	18204-18212	VBP	denotes	interact
T5178	18213-18217	IN	denotes	with
T5179	18218-18221	DT	denotes	the
T5181	18222-18223	NN	denotes	C
T5183	18223-18224	HYPH	denotes	-
T5182	18224-18232	JJ	denotes	terminal
T5180	18233-18240	NN	denotes	peptide
T5184	18241-18246	IN	denotes	while
T5186	18247-18253	NN	denotes	TRIM11
T5185	18254-18263	VBZ	denotes	interacts
T5187	18264-18268	IN	denotes	with
T5188	18269-18270	DT	denotes	a
T5190	18271-18275	RBR	denotes	more
T5192	18276-18277	NN	denotes	N
T5193	18277-18278	HYPH	denotes	-
T5191	18278-18286	JJ	denotes	terminal
T5189	18287-18291	NN	denotes	part
T5194	18292-18294	IN	denotes	of
T5195	18295-18298	DT	denotes	the
T5197	18299-18302	NN	denotes	PST
T5196	18303-18309	NN	denotes	domain
T5198	18309-18310	.	denotes	.
T5199	18310-18413	sentence	denotes	The interactions between the PST domain and HOMER3, DNCL1 and TRIM11 were confirmed in pairwise tests.
T5200	18311-18314	DT	denotes	The
T5201	18315-18327	NNS	denotes	interactions
T5203	18328-18335	IN	denotes	between
T5204	18336-18339	DT	denotes	the
T5206	18340-18343	NN	denotes	PST
T5205	18344-18350	NN	denotes	domain
T5207	18351-18354	CC	denotes	and
T5208	18355-18361	NN	denotes	HOMER3
T5209	18361-18363	,	denotes	,
T5210	18363-18368	NN	denotes	DNCL1
T5211	18369-18372	CC	denotes	and
T5212	18373-18379	NN	denotes	TRIM11
T5213	18380-18384	VBD	denotes	were
T5202	18385-18394	VBN	denotes	confirmed
T5214	18395-18397	IN	denotes	in
T5215	18398-18406	JJ	denotes	pairwise
T5216	18407-18412	NNS	denotes	tests
T5217	18412-18413	.	denotes	.
T5218	18413-18738	sentence	denotes	We were not able to confirm the interaction between HOMER3 or DNCL1 with the C-terminal peptide construct in pairwise tests, but we showed that the C-terminal peptide was important for PAX6/HOMER3 or PAX6/DNCL1 interaction because HOMER3 and DNCL1 did not interact with a PST domain construct lacking the C-terminal peptide.
T5219	18414-18416	PRP	denotes	We
T5220	18417-18421	VBD	denotes	were
T5221	18422-18425	RB	denotes	not
T5222	18426-18430	JJ	denotes	able
T5223	18431-18433	TO	denotes	to
T5224	18434-18441	VB	denotes	confirm
T5225	18442-18445	DT	denotes	the
T5226	18446-18457	NN	denotes	interaction
T5227	18458-18465	IN	denotes	between
T5228	18466-18472	NN	denotes	HOMER3
T5229	18473-18475	CC	denotes	or
T5230	18476-18481	NN	denotes	DNCL1
T5231	18482-18486	IN	denotes	with
T5232	18487-18490	DT	denotes	the
T5234	18491-18492	NN	denotes	C
T5236	18492-18493	HYPH	denotes	-
T5235	18493-18501	JJ	denotes	terminal
T5237	18502-18509	NN	denotes	peptide
T5233	18510-18519	NN	denotes	construct
T5238	18520-18522	IN	denotes	in
T5239	18523-18531	JJ	denotes	pairwise
T5240	18532-18537	NNS	denotes	tests
T5241	18537-18539	,	denotes	,
T5242	18539-18542	CC	denotes	but
T5243	18543-18545	PRP	denotes	we
T5244	18546-18552	VBD	denotes	showed
T5245	18553-18557	IN	denotes	that
T5247	18558-18561	DT	denotes	the
T5249	18562-18563	NN	denotes	C
T5251	18563-18564	HYPH	denotes	-
T5250	18564-18572	JJ	denotes	terminal
T5248	18573-18580	NN	denotes	peptide
T5246	18581-18584	VBD	denotes	was
T5252	18585-18594	JJ	denotes	important
T5253	18595-18598	IN	denotes	for
T5254	18599-18603	NN	denotes	PAX6
T5256	18603-18604	HYPH	denotes	/
T5255	18604-18610	NN	denotes	HOMER3
T5258	18611-18613	CC	denotes	or
T5259	18614-18618	NN	denotes	PAX6
T5261	18618-18619	HYPH	denotes	/
T5260	18619-18624	NN	denotes	DNCL1
T5257	18625-18636	NN	denotes	interaction
T5262	18637-18644	IN	denotes	because
T5264	18645-18651	NN	denotes	HOMER3
T5265	18652-18655	CC	denotes	and
T5266	18656-18661	NN	denotes	DNCL1
T5267	18662-18665	VBD	denotes	did
T5268	18666-18669	RB	denotes	not
T5263	18670-18678	VB	denotes	interact
T5269	18679-18683	IN	denotes	with
T5270	18684-18685	DT	denotes	a
T5272	18686-18689	NN	denotes	PST
T5273	18690-18696	NN	denotes	domain
T5271	18697-18706	NN	denotes	construct
T5274	18707-18714	VBG	denotes	lacking
T5275	18715-18718	DT	denotes	the
T5277	18719-18720	NN	denotes	C
T5279	18720-18721	HYPH	denotes	-
T5278	18721-18729	JJ	denotes	terminal
T5276	18730-18737	NN	denotes	peptide
T5280	18737-18738	.	denotes	.
T5281	18738-18948	sentence	denotes	HOMER3 is found in the PSD of neurons and directly binds to type I metabotropic glutamate receptors, which act via phospholipase C to stimulate IP3-mediated release of Ca2+ from intracellular vesicles [35,39].
T5282	18739-18745	NN	denotes	HOMER3
T5284	18746-18748	VBZ	denotes	is
T5283	18749-18754	VBN	denotes	found
T5285	18755-18757	IN	denotes	in
T5286	18758-18761	DT	denotes	the
T5287	18762-18765	NN	denotes	PSD
T5288	18766-18768	IN	denotes	of
T5289	18769-18776	NNS	denotes	neurons
T5290	18777-18780	CC	denotes	and
T5291	18781-18789	RB	denotes	directly
T5292	18790-18795	VBZ	denotes	binds
T5293	18796-18798	IN	denotes	to
T5294	18799-18803	NN	denotes	type
T5296	18804-18805	CD	denotes	I
T5297	18806-18818	JJ	denotes	metabotropic
T5298	18819-18828	NN	denotes	glutamate
T5295	18829-18838	NNS	denotes	receptors
T5299	18838-18840	,	denotes	,
T5300	18840-18845	WDT	denotes	which
T5301	18846-18849	VBP	denotes	act
T5302	18850-18853	IN	denotes	via
T5303	18854-18867	NN	denotes	phospholipase
T5304	18868-18869	NN	denotes	C
T5305	18870-18872	TO	denotes	to
T5306	18873-18882	VB	denotes	stimulate
T5307	18883-18886	NN	denotes	IP3
T5309	18886-18887	HYPH	denotes	-
T5308	18887-18895	VBN	denotes	mediated
T5310	18896-18903	NN	denotes	release
T5311	18904-18906	IN	denotes	of
T5312	18907-18911	NN	denotes	Ca2+
T5313	18912-18916	IN	denotes	from
T5314	18917-18930	JJ	denotes	intracellular
T5315	18931-18939	NNS	denotes	vesicles
T5316	18940-18941	-LRB-	denotes	[
T5318	18941-18943	CD	denotes	35
T5319	18943-18944	,	denotes	,
T5317	18944-18946	CD	denotes	39
T5320	18946-18947	-RRB-	denotes	]
T5321	18947-18948	.	denotes	.
T5322	18948-19208	sentence	denotes	HOMER3 is a member of the HOMER family of proteins that are constitutively expressed in the brain and play a role in post-synaptic signalling and receptor trafficking by forming multivalent links with various receptors and PSD scaffolding proteins [35,39-41].
T5323	18949-18955	NN	denotes	HOMER3
T5324	18956-18958	VBZ	denotes	is
T5325	18959-18960	DT	denotes	a
T5326	18961-18967	NN	denotes	member
T5327	18968-18970	IN	denotes	of
T5328	18971-18974	DT	denotes	the
T5330	18975-18980	NN	denotes	HOMER
T5329	18981-18987	NN	denotes	family
T5331	18988-18990	IN	denotes	of
T5332	18991-18999	NN	denotes	proteins
T5333	19000-19004	WDT	denotes	that
T5335	19005-19008	VBP	denotes	are
T5336	19009-19023	RB	denotes	constitutively
T5334	19024-19033	VBN	denotes	expressed
T5337	19034-19036	IN	denotes	in
T5338	19037-19040	DT	denotes	the
T5339	19041-19046	NN	denotes	brain
T5340	19047-19050	CC	denotes	and
T5341	19051-19055	VBP	denotes	play
T5342	19056-19057	DT	denotes	a
T5343	19058-19062	NN	denotes	role
T5344	19063-19065	IN	denotes	in
T5345	19066-19079	JJ	denotes	post-synaptic
T5346	19080-19090	NN	denotes	signalling
T5347	19091-19094	CC	denotes	and
T5348	19095-19103	NN	denotes	receptor
T5349	19104-19115	NN	denotes	trafficking
T5350	19116-19118	IN	denotes	by
T5351	19119-19126	VBG	denotes	forming
T5352	19127-19138	JJ	denotes	multivalent
T5353	19139-19144	NNS	denotes	links
T5354	19145-19149	IN	denotes	with
T5355	19150-19157	JJ	denotes	various
T5356	19158-19167	NNS	denotes	receptors
T5357	19168-19171	CC	denotes	and
T5358	19172-19175	NN	denotes	PSD
T5360	19176-19187	NN	denotes	scaffolding
T5359	19188-19196	NN	denotes	proteins
T5361	19197-19198	-LRB-	denotes	[
T5362	19198-19200	CD	denotes	35
T5363	19200-19201	,	denotes	,
T5364	19201-19203	CD	denotes	39
T5365	19203-19204	SYM	denotes	-
T5366	19204-19206	CD	denotes	41
T5367	19206-19207	-RRB-	denotes	]
T5368	19207-19208	.	denotes	.
T5369	19208-19297	sentence	denotes	HOMER proteins have also been implicated in axon guidance during brain development [42].
T5370	19209-19214	NN	denotes	HOMER
T5371	19215-19223	NN	denotes	proteins
T5373	19224-19228	VBP	denotes	have
T5374	19229-19233	RB	denotes	also
T5375	19234-19238	VBN	denotes	been
T5372	19239-19249	VBN	denotes	implicated
T5376	19250-19252	IN	denotes	in
T5377	19253-19257	NN	denotes	axon
T5378	19258-19266	NN	denotes	guidance
T5379	19267-19273	IN	denotes	during
T5380	19274-19279	NN	denotes	brain
T5381	19280-19291	NN	denotes	development
T5382	19292-19293	-LRB-	denotes	[
T5383	19293-19295	CD	denotes	42
T5384	19295-19296	-RRB-	denotes	]
T5385	19296-19297	.	denotes	.
T5386	19297-19393	sentence	denotes	DNCL1 is a subunit of two intracellular transport protein complexes, dynein and myosin Va [36].
T5387	19298-19303	NN	denotes	DNCL1
T5388	19304-19306	VBZ	denotes	is
T5389	19307-19308	DT	denotes	a
T5390	19309-19316	NN	denotes	subunit
T5391	19317-19319	IN	denotes	of
T5392	19320-19323	CD	denotes	two
T5394	19324-19337	JJ	denotes	intracellular
T5395	19338-19347	NN	denotes	transport
T5396	19348-19355	NN	denotes	protein
T5393	19356-19365	NNS	denotes	complexes
T5397	19365-19367	,	denotes	,
T5398	19367-19373	NN	denotes	dynein
T5399	19374-19377	CC	denotes	and
T5400	19378-19384	NN	denotes	myosin
T5401	19385-19387	NN	denotes	Va
T5402	19388-19389	-LRB-	denotes	[
T5403	19389-19391	CD	denotes	36
T5404	19391-19392	-RRB-	denotes	]
T5405	19392-19393	.	denotes	.
T5406	19393-19547	sentence	denotes	Dynein and myosin Va are involved in the microtubule-based and actin-based movement respectively of proteins, organelles and vesicles in neurons [36,37].
T5407	19394-19400	NN	denotes	Dynein
T5409	19401-19404	CC	denotes	and
T5410	19405-19411	NN	denotes	myosin
T5411	19412-19414	NN	denotes	Va
T5412	19415-19418	VBP	denotes	are
T5408	19419-19427	VBN	denotes	involved
T5413	19428-19430	IN	denotes	in
T5414	19431-19434	DT	denotes	the
T5416	19435-19446	NN	denotes	microtubule
T5418	19446-19447	HYPH	denotes	-
T5417	19447-19452	VBN	denotes	based
T5419	19453-19456	CC	denotes	and
T5420	19457-19462	NN	denotes	actin
T5422	19462-19463	HYPH	denotes	-
T5421	19463-19468	VBN	denotes	based
T5415	19469-19477	NN	denotes	movement
T5423	19478-19490	RB	denotes	respectively
T5424	19491-19493	IN	denotes	of
T5425	19494-19502	NN	denotes	proteins
T5426	19502-19504	,	denotes	,
T5427	19504-19514	NNS	denotes	organelles
T5428	19515-19518	CC	denotes	and
T5429	19519-19527	NNS	denotes	vesicles
T5430	19528-19530	IN	denotes	in
T5431	19531-19538	NNS	denotes	neurons
T5432	19539-19540	-LRB-	denotes	[
T5434	19540-19542	CD	denotes	36
T5435	19542-19543	,	denotes	,
T5433	19543-19545	CD	denotes	37
T5436	19545-19546	-RRB-	denotes	]
T5437	19546-19547	.	denotes	.
T5438	19547-19730	sentence	denotes	Myosin Va is enriched in the PSD [43], and DNCL1 binds to a variety of PSD proteins including guanylate kinase domain-associated protein [44] and neuronal nitric oxide synthase [45].
T5439	19548-19554	NN	denotes	Myosin
T5440	19555-19557	NN	denotes	Va
T5442	19558-19560	VBZ	denotes	is
T5441	19561-19569	VBN	denotes	enriched
T5443	19570-19572	IN	denotes	in
T5444	19573-19576	DT	denotes	the
T5445	19577-19580	NN	denotes	PSD
T5446	19581-19582	-LRB-	denotes	[
T5447	19582-19584	CD	denotes	43
T5448	19584-19585	-RRB-	denotes	]
T5449	19585-19587	,	denotes	,
T5450	19587-19590	CC	denotes	and
T5451	19591-19596	NN	denotes	DNCL1
T5452	19597-19602	VBZ	denotes	binds
T5453	19603-19605	IN	denotes	to
T5454	19606-19607	DT	denotes	a
T5455	19608-19615	NN	denotes	variety
T5456	19616-19618	IN	denotes	of
T5457	19619-19622	NN	denotes	PSD
T5458	19623-19631	NN	denotes	proteins
T5459	19632-19641	VBG	denotes	including
T5460	19642-19651	NN	denotes	guanylate
T5462	19652-19658	NN	denotes	kinase
T5463	19659-19665	NN	denotes	domain
T5465	19665-19666	HYPH	denotes	-
T5464	19666-19676	VBN	denotes	associated
T5461	19677-19684	NN	denotes	protein
T5466	19685-19686	-LRB-	denotes	[
T5467	19686-19688	CD	denotes	44
T5468	19688-19689	-RRB-	denotes	]
T5469	19690-19693	CC	denotes	and
T5470	19694-19702	JJ	denotes	neuronal
T5472	19703-19709	JJ	denotes	nitric
T5473	19710-19715	NN	denotes	oxide
T5471	19716-19724	NN	denotes	synthase
T5474	19725-19726	-LRB-	denotes	[
T5475	19726-19728	CD	denotes	45
T5476	19728-19729	-RRB-	denotes	]
T5477	19729-19730	.	denotes	.
T5478	19730-20049	sentence	denotes	TRIM11 is a member of the mouse tripartite motif protein family (also known as the RBCC family), and contains the three characteristic structural motifs of this protein family, a RING finger, a B-box zinc finger, and a coiled coil domain, as well as a B30.2 domain that is found in many but not all TRIM proteins [38].
T5479	19731-19737	NN	denotes	TRIM11
T5480	19738-19740	VBZ	denotes	is
T5481	19741-19742	DT	denotes	a
T5482	19743-19749	NN	denotes	member
T5483	19750-19752	IN	denotes	of
T5484	19753-19756	DT	denotes	the
T5486	19757-19762	NN	denotes	mouse
T5487	19763-19773	JJ	denotes	tripartite
T5488	19774-19779	NN	denotes	motif
T5489	19780-19787	NN	denotes	protein
T5485	19788-19794	NN	denotes	family
T5490	19795-19796	-LRB-	denotes	(
T5491	19796-19800	RB	denotes	also
T5492	19801-19806	VBN	denotes	known
T5493	19807-19809	IN	denotes	as
T5494	19810-19813	DT	denotes	the
T5496	19814-19818	NN	denotes	RBCC
T5495	19819-19825	NN	denotes	family
T5497	19825-19826	-RRB-	denotes	)
T5498	19826-19828	,	denotes	,
T5499	19828-19831	CC	denotes	and
T5500	19832-19840	VBZ	denotes	contains
T5501	19841-19844	DT	denotes	the
T5503	19845-19850	CD	denotes	three
T5504	19851-19865	JJ	denotes	characteristic
T5505	19866-19876	JJ	denotes	structural
T5502	19877-19883	NNS	denotes	motifs
T5506	19884-19886	IN	denotes	of
T5507	19887-19891	DT	denotes	this
T5509	19892-19899	NN	denotes	protein
T5508	19900-19906	NN	denotes	family
T5510	19906-19908	,	denotes	,
T5511	19908-19909	DT	denotes	a
T5513	19910-19914	NN	denotes	RING
T5512	19915-19921	NN	denotes	finger
T5514	19921-19923	,	denotes	,
T5515	19923-19924	DT	denotes	a
T5517	19925-19926	NN	denotes	B
T5519	19926-19927	HYPH	denotes	-
T5518	19927-19930	NN	denotes	box
T5520	19931-19935	NN	denotes	zinc
T5516	19936-19942	NN	denotes	finger
T5521	19942-19944	,	denotes	,
T5522	19944-19947	CC	denotes	and
T5523	19948-19949	DT	denotes	a
T5525	19950-19956	VBN	denotes	coiled
T5526	19957-19961	NN	denotes	coil
T5524	19962-19968	NN	denotes	domain
T5527	19968-19970	,	denotes	,
T5528	19970-19972	RB	denotes	as
T5530	19973-19977	RB	denotes	well
T5529	19978-19980	IN	denotes	as
T5531	19981-19982	DT	denotes	a
T5533	19983-19988	NN	denotes	B30.2
T5532	19989-19995	NN	denotes	domain
T5534	19996-20000	WDT	denotes	that
T5536	20001-20003	VBZ	denotes	is
T5535	20004-20009	VBN	denotes	found
T5537	20010-20012	IN	denotes	in
T5538	20013-20017	JJ	denotes	many
T5539	20018-20021	CC	denotes	but
T5540	20022-20025	RB	denotes	not
T5541	20026-20029	DT	denotes	all
T5543	20030-20034	NN	denotes	TRIM
T5542	20035-20043	NN	denotes	proteins
T5544	20044-20045	-LRB-	denotes	[
T5545	20045-20047	CD	denotes	38
T5546	20047-20048	-RRB-	denotes	]
T5547	20048-20049	.	denotes	.
T5548	20049-20166	sentence	denotes	TRIM11 interacts with Humanin, a protein that suppresses the neurotoxicity associated with Alzheimer's disease [46].
T5549	20050-20056	NN	denotes	TRIM11
T5550	20057-20066	VBZ	denotes	interacts
T5551	20067-20071	IN	denotes	with
T5552	20072-20079	NN	denotes	Humanin
T5553	20079-20081	,	denotes	,
T5554	20081-20082	DT	denotes	a
T5555	20083-20090	NN	denotes	protein
T5556	20091-20095	WDT	denotes	that
T5557	20096-20106	VBZ	denotes	suppresses
T5558	20107-20110	DT	denotes	the
T5559	20111-20124	NN	denotes	neurotoxicity
T5560	20125-20135	VBN	denotes	associated
T5561	20136-20140	IN	denotes	with
T5562	20141-20150	NNP	denotes	Alzheimer
T5564	20150-20152	POS	denotes	's
T5563	20153-20160	NN	denotes	disease
T5565	20161-20162	-LRB-	denotes	[
T5566	20162-20164	CD	denotes	46
T5567	20164-20165	-RRB-	denotes	]
T5568	20165-20166	.	denotes	.
T5569	20166-20282	sentence	denotes	TRIM11 lowers Humanin levels by a mechanism that appears to involve ubiqutin-mediated proteasomal degradation [46].
T5570	20167-20173	NN	denotes	TRIM11
T5571	20174-20180	VBZ	denotes	lowers
T5572	20181-20188	NN	denotes	Humanin
T5573	20189-20195	NNS	denotes	levels
T5574	20196-20198	IN	denotes	by
T5575	20199-20200	DT	denotes	a
T5576	20201-20210	NN	denotes	mechanism
T5577	20211-20215	WDT	denotes	that
T5578	20216-20223	VBZ	denotes	appears
T5579	20224-20226	TO	denotes	to
T5580	20227-20234	VB	denotes	involve
T5581	20235-20243	NN	denotes	ubiqutin
T5583	20243-20244	HYPH	denotes	-
T5582	20244-20252	VBN	denotes	mediated
T5585	20253-20264	JJ	denotes	proteasomal
T5584	20265-20276	NN	denotes	degradation
T5586	20277-20278	-LRB-	denotes	[
T5587	20278-20280	CD	denotes	46
T5588	20280-20281	-RRB-	denotes	]
T5589	20281-20282	.	denotes	.
T5590	20282-20405	sentence	denotes	At present our data must be considered preliminary because the interactions have not been confirmed by any other approach.
T5591	20283-20285	IN	denotes	At
T5593	20286-20293	JJ	denotes	present
T5594	20294-20297	PRP$	denotes	our
T5595	20298-20302	NNS	denotes	data
T5596	20303-20307	MD	denotes	must
T5597	20308-20310	VB	denotes	be
T5592	20311-20321	VBN	denotes	considered
T5598	20322-20333	JJ	denotes	preliminary
T5599	20334-20341	IN	denotes	because
T5601	20342-20345	DT	denotes	the
T5602	20346-20358	NNS	denotes	interactions
T5603	20359-20363	VBP	denotes	have
T5604	20364-20367	RB	denotes	not
T5605	20368-20372	VBN	denotes	been
T5600	20373-20382	VBN	denotes	confirmed
T5606	20383-20385	IN	denotes	by
T5607	20386-20389	DT	denotes	any
T5609	20390-20395	JJ	denotes	other
T5608	20396-20404	NN	denotes	approach
T5610	20404-20405	.	denotes	.
T5611	20405-20591	sentence	denotes	However it is interesting to speculate that the interaction of PAX6 with HOMER3 and DNCL1 may be the basis of a mechanism by which synaptic signalling causes changes in gene expression.
T5612	20406-20413	RB	denotes	However
T5614	20414-20416	PRP	denotes	it
T5613	20417-20419	VBZ	denotes	is
T5615	20420-20431	JJ	denotes	interesting
T5616	20432-20434	TO	denotes	to
T5617	20435-20444	VB	denotes	speculate
T5618	20445-20449	IN	denotes	that
T5620	20450-20453	DT	denotes	the
T5621	20454-20465	NN	denotes	interaction
T5622	20466-20468	IN	denotes	of
T5623	20469-20473	NN	denotes	PAX6
T5624	20474-20478	IN	denotes	with
T5625	20479-20485	NN	denotes	HOMER3
T5626	20486-20489	CC	denotes	and
T5627	20490-20495	NN	denotes	DNCL1
T5628	20496-20499	MD	denotes	may
T5619	20500-20502	VB	denotes	be
T5629	20503-20506	DT	denotes	the
T5630	20507-20512	NN	denotes	basis
T5631	20513-20515	IN	denotes	of
T5632	20516-20517	DT	denotes	a
T5633	20518-20527	NN	denotes	mechanism
T5634	20528-20530	IN	denotes	by
T5636	20531-20536	WDT	denotes	which
T5637	20537-20545	JJ	denotes	synaptic
T5638	20546-20556	NN	denotes	signalling
T5635	20557-20563	VBZ	denotes	causes
T5639	20564-20571	NNS	denotes	changes
T5640	20572-20574	IN	denotes	in
T5641	20575-20579	NN	denotes	gene
T5642	20580-20590	NN	denotes	expression
T5643	20590-20591	.	denotes	.
T5644	20591-20660	sentence	denotes	We propose that PAX6 is sequestered in the PSD by binding to HOMER3.
T5645	20592-20594	PRP	denotes	We
T5646	20595-20602	VBP	denotes	propose
T5647	20603-20607	IN	denotes	that
T5649	20608-20612	NN	denotes	PAX6
T5650	20613-20615	VBZ	denotes	is
T5648	20616-20627	VBN	denotes	sequestered
T5651	20628-20630	IN	denotes	in
T5652	20631-20634	DT	denotes	the
T5653	20635-20638	NN	denotes	PSD
T5654	20639-20641	IN	denotes	by
T5655	20642-20649	VBG	denotes	binding
T5656	20650-20652	IN	denotes	to
T5657	20653-20659	NN	denotes	HOMER3
T5658	20659-20660	.	denotes	.
T5659	20660-20821	sentence	denotes	Since receptor activation causes dissociation of HOMER proteins [35], PAX6 may be released as a result of synaptic activity, allowing it to interact with DNCL1.
T5660	20661-20666	IN	denotes	Since
T5662	20667-20675	NN	denotes	receptor
T5663	20676-20686	NN	denotes	activation
T5661	20687-20693	VBZ	denotes	causes
T5665	20694-20706	NN	denotes	dissociation
T5666	20707-20709	IN	denotes	of
T5667	20710-20715	NN	denotes	HOMER
T5668	20716-20724	NN	denotes	proteins
T5669	20725-20726	-LRB-	denotes	[
T5670	20726-20728	CD	denotes	35
T5671	20728-20729	-RRB-	denotes	]
T5672	20729-20731	,	denotes	,
T5673	20731-20735	NN	denotes	PAX6
T5674	20736-20739	MD	denotes	may
T5675	20740-20742	VB	denotes	be
T5664	20743-20751	VBN	denotes	released
T5676	20752-20754	IN	denotes	as
T5677	20755-20756	DT	denotes	a
T5678	20757-20763	NN	denotes	result
T5679	20764-20766	IN	denotes	of
T5680	20767-20775	JJ	denotes	synaptic
T5681	20776-20784	NN	denotes	activity
T5682	20784-20786	,	denotes	,
T5683	20786-20794	VBG	denotes	allowing
T5684	20795-20797	PRP	denotes	it
T5686	20798-20800	TO	denotes	to
T5685	20801-20809	VB	denotes	interact
T5687	20810-20814	IN	denotes	with
T5688	20815-20820	NN	denotes	DNCL1
T5689	20820-20821	.	denotes	.
T5690	20821-21050	sentence	denotes	The PAX6/DNCL1 complex could then participate in myosin Va-mediated transport along the PSD-associated actin cytoskeleton followed by dynein-mediated transport along the microtubule network, eventually reaching the nucleus [36].
T5691	20822-20825	DT	denotes	The
T5693	20826-20830	NN	denotes	PAX6
T5695	20830-20831	HYPH	denotes	/
T5694	20831-20836	NN	denotes	DNCL1
T5692	20837-20844	NN	denotes	complex
T5697	20845-20850	MD	denotes	could
T5698	20851-20855	RB	denotes	then
T5696	20856-20867	VB	denotes	participate
T5699	20868-20870	IN	denotes	in
T5700	20871-20877	NN	denotes	myosin
T5701	20878-20880	NN	denotes	Va
T5703	20880-20881	HYPH	denotes	-
T5702	20881-20889	VBN	denotes	mediated
T5704	20890-20899	NN	denotes	transport
T5705	20900-20905	IN	denotes	along
T5706	20906-20909	DT	denotes	the
T5708	20910-20913	NN	denotes	PSD
T5710	20913-20914	HYPH	denotes	-
T5709	20914-20924	VBN	denotes	associated
T5711	20925-20930	NN	denotes	actin
T5707	20931-20943	NN	denotes	cytoskeleton
T5712	20944-20952	VBN	denotes	followed
T5713	20953-20955	IN	denotes	by
T5714	20956-20962	NN	denotes	dynein
T5716	20962-20963	HYPH	denotes	-
T5715	20963-20971	VBN	denotes	mediated
T5717	20972-20981	NN	denotes	transport
T5718	20982-20987	IN	denotes	along
T5719	20988-20991	DT	denotes	the
T5721	20992-21003	NN	denotes	microtubule
T5720	21004-21011	NN	denotes	network
T5722	21011-21013	,	denotes	,
T5723	21013-21023	RB	denotes	eventually
T5724	21024-21032	VBG	denotes	reaching
T5725	21033-21036	DT	denotes	the
T5726	21037-21044	NN	denotes	nucleus
T5727	21045-21046	-LRB-	denotes	[
T5728	21046-21048	CD	denotes	36
T5729	21048-21049	-RRB-	denotes	]
T5730	21049-21050	.	denotes	.
T5731	21050-21151	sentence	denotes	Since TRIM11 is implicated in protein degradation [46], it may play a role in PAX6 protein turnover.
T5732	21051-21056	IN	denotes	Since
T5734	21057-21063	NN	denotes	TRIM11
T5735	21064-21066	VBZ	denotes	is
T5733	21067-21077	VBN	denotes	implicated
T5737	21078-21080	IN	denotes	in
T5738	21081-21088	NN	denotes	protein
T5739	21089-21100	NN	denotes	degradation
T5740	21101-21102	-LRB-	denotes	[
T5741	21102-21104	CD	denotes	46
T5742	21104-21105	-RRB-	denotes	]
T5743	21105-21107	,	denotes	,
T5744	21107-21109	PRP	denotes	it
T5745	21110-21113	MD	denotes	may
T5736	21114-21118	VB	denotes	play
T5746	21119-21120	DT	denotes	a
T5747	21121-21125	NN	denotes	role
T5748	21126-21128	IN	denotes	in
T5749	21129-21133	NN	denotes	PAX6
T5750	21134-21141	NN	denotes	protein
T5751	21142-21150	NN	denotes	turnover
T5752	21150-21151	.	denotes	.
T5753	21151-21327	sentence	denotes	Precedents for association of transcription factors with the post-synaptic density include STAT3 and CREB, which act as messengers between the synapse and the nucleus [47,48].
T5754	21152-21162	NNS	denotes	Precedents
T5756	21163-21166	IN	denotes	for
T5757	21167-21178	NN	denotes	association
T5758	21179-21181	IN	denotes	of
T5759	21182-21195	NN	denotes	transcription
T5760	21196-21203	NNS	denotes	factors
T5761	21204-21208	IN	denotes	with
T5762	21209-21212	DT	denotes	the
T5764	21213-21226	JJ	denotes	post-synaptic
T5763	21227-21234	NN	denotes	density
T5755	21235-21242	VBP	denotes	include
T5765	21243-21248	NN	denotes	STAT3
T5766	21249-21252	CC	denotes	and
T5767	21253-21257	NN	denotes	CREB
T5768	21257-21259	,	denotes	,
T5769	21259-21264	WDT	denotes	which
T5770	21265-21268	VBP	denotes	act
T5771	21269-21271	IN	denotes	as
T5772	21272-21282	NNS	denotes	messengers
T5773	21283-21290	IN	denotes	between
T5774	21291-21294	DT	denotes	the
T5775	21295-21302	NN	denotes	synapse
T5776	21303-21306	CC	denotes	and
T5777	21307-21310	DT	denotes	the
T5778	21311-21318	NN	denotes	nucleus
T5779	21319-21320	-LRB-	denotes	[
T5781	21320-21322	CD	denotes	47
T5782	21322-21323	,	denotes	,
T5780	21323-21325	CD	denotes	48
T5783	21325-21326	-RRB-	denotes	]
T5784	21326-21327	.	denotes	.
T5785	21327-21574	sentence	denotes	Although the full-length PAX6 protein is predominantly nuclear, there is good evidence in mouse, quail and nematode for an isoform that lacks the paired domain, is both nuclear and cytoplasmic, and binds DNA through the homeodomain alone [49-51].
T5786	21328-21336	IN	denotes	Although
T5788	21337-21340	DT	denotes	the
T5790	21341-21345	JJ	denotes	full
T5792	21345-21346	HYPH	denotes	-
T5791	21346-21352	NN	denotes	length
T5793	21353-21357	NN	denotes	PAX6
T5789	21358-21365	NN	denotes	protein
T5787	21366-21368	VBZ	denotes	is
T5795	21369-21382	RB	denotes	predominantly
T5796	21383-21390	JJ	denotes	nuclear
T5797	21390-21392	,	denotes	,
T5798	21392-21397	EX	denotes	there
T5794	21398-21400	VBZ	denotes	is
T5799	21401-21405	JJ	denotes	good
T5800	21406-21414	NN	denotes	evidence
T5801	21415-21417	IN	denotes	in
T5802	21418-21423	NN	denotes	mouse
T5803	21423-21425	,	denotes	,
T5804	21425-21430	NN	denotes	quail
T5805	21431-21434	CC	denotes	and
T5806	21435-21443	NN	denotes	nematode
T5807	21444-21447	IN	denotes	for
T5808	21448-21450	DT	denotes	an
T5809	21451-21458	NN	denotes	isoform
T5810	21459-21463	WDT	denotes	that
T5811	21464-21469	VBZ	denotes	lacks
T5812	21470-21473	DT	denotes	the
T5814	21474-21480	JJ	denotes	paired
T5813	21481-21487	NN	denotes	domain
T5815	21487-21489	,	denotes	,
T5816	21489-21491	VBZ	denotes	is
T5817	21492-21496	CC	denotes	both
T5818	21497-21504	JJ	denotes	nuclear
T5819	21505-21508	CC	denotes	and
T5820	21509-21520	JJ	denotes	cytoplasmic
T5821	21520-21522	,	denotes	,
T5822	21522-21525	CC	denotes	and
T5823	21526-21531	VBZ	denotes	binds
T5824	21532-21535	NN	denotes	DNA
T5825	21536-21543	IN	denotes	through
T5826	21544-21547	DT	denotes	the
T5827	21548-21559	NN	denotes	homeodomain
T5828	21560-21565	RB	denotes	alone
T5829	21566-21567	-LRB-	denotes	[
T5830	21567-21569	CD	denotes	49
T5831	21569-21570	SYM	denotes	-
T5832	21570-21572	CD	denotes	51
T5833	21572-21573	-RRB-	denotes	]
T5834	21573-21574	.	denotes	.
T5835	21574-21774	sentence	denotes	In mice the paired-less isoform is relatively abundant in brain [49]; however its subcellular localisation in neurones, and the possibility of an association with the PSD, remains to be investigated.
T5836	21575-21577	IN	denotes	In
T5838	21578-21582	NNS	denotes	mice
T5839	21583-21586	DT	denotes	the
T5841	21587-21593	VBN	denotes	paired
T5843	21593-21594	HYPH	denotes	-
T5842	21594-21598	JJ	denotes	less
T5840	21599-21606	NN	denotes	isoform
T5844	21607-21609	VBZ	denotes	is
T5845	21610-21620	RB	denotes	relatively
T5846	21621-21629	JJ	denotes	abundant
T5847	21630-21632	IN	denotes	in
T5848	21633-21638	NN	denotes	brain
T5849	21639-21640	-LRB-	denotes	[
T5850	21640-21642	CD	denotes	49
T5851	21642-21643	-RRB-	denotes	]
T5852	21643-21644	:	denotes	;
T5853	21645-21652	RB	denotes	however
T5854	21653-21656	PRP$	denotes	its
T5856	21657-21668	JJ	denotes	subcellular
T5855	21669-21681	NN	denotes	localisation
T5857	21682-21684	IN	denotes	in
T5858	21685-21693	NNS	denotes	neurones
T5859	21693-21695	,	denotes	,
T5860	21695-21698	CC	denotes	and
T5861	21699-21702	DT	denotes	the
T5862	21703-21714	NN	denotes	possibility
T5863	21715-21717	IN	denotes	of
T5864	21718-21720	DT	denotes	an
T5865	21721-21732	NN	denotes	association
T5866	21733-21737	IN	denotes	with
T5867	21738-21741	DT	denotes	the
T5868	21742-21745	NN	denotes	PSD
T5869	21745-21747	,	denotes	,
T5837	21747-21754	VBZ	denotes	remains
T5870	21755-21757	TO	denotes	to
T5872	21758-21760	VB	denotes	be
T5871	21761-21773	VBN	denotes	investigated
T5873	21773-21774	.	denotes	.
T5874	21774-22071	sentence	denotes	Regarding the subcellular localisation of the putative interacting proteins, HOMER3 is predominantly found at the interface between the PSD and the cytoplasm [39], DNCL1 is chiefly cytoplasmic, although nuclear localisation has been reported [51], and TRIM11 is both nuclear and cytoplasmic [38].
T5875	21775-21784	VBG	denotes	Regarding
T5877	21785-21788	DT	denotes	the
T5879	21789-21800	JJ	denotes	subcellular
T5878	21801-21813	NN	denotes	localisation
T5880	21814-21816	IN	denotes	of
T5881	21817-21820	DT	denotes	the
T5883	21821-21829	JJ	denotes	putative
T5884	21830-21841	VBG	denotes	interacting
T5882	21842-21850	NN	denotes	proteins
T5885	21850-21852	,	denotes	,
T5886	21852-21858	NN	denotes	HOMER3
T5887	21859-21861	VBZ	denotes	is
T5888	21862-21875	RB	denotes	predominantly
T5876	21876-21881	VBN	denotes	found
T5889	21882-21884	IN	denotes	at
T5890	21885-21888	DT	denotes	the
T5891	21889-21898	NN	denotes	interface
T5892	21899-21906	IN	denotes	between
T5893	21907-21910	DT	denotes	the
T5894	21911-21914	NN	denotes	PSD
T5895	21915-21918	CC	denotes	and
T5896	21919-21922	DT	denotes	the
T5897	21923-21932	NN	denotes	cytoplasm
T5898	21933-21934	-LRB-	denotes	[
T5899	21934-21936	CD	denotes	39
T5900	21936-21937	-RRB-	denotes	]
T5901	21937-21939	,	denotes	,
T5902	21939-21944	NN	denotes	DNCL1
T5903	21945-21947	VBZ	denotes	is
T5904	21948-21955	RB	denotes	chiefly
T5905	21956-21967	JJ	denotes	cytoplasmic
T5906	21967-21969	,	denotes	,
T5907	21969-21977	IN	denotes	although
T5909	21978-21985	JJ	denotes	nuclear
T5910	21986-21998	NN	denotes	localisation
T5911	21999-22002	VBZ	denotes	has
T5912	22003-22007	VBN	denotes	been
T5908	22008-22016	VBN	denotes	reported
T5913	22017-22018	-LRB-	denotes	[
T5914	22018-22020	CD	denotes	51
T5915	22020-22021	-RRB-	denotes	]
T5916	22021-22023	,	denotes	,
T5917	22023-22026	CC	denotes	and
T5918	22027-22033	NN	denotes	TRIM11
T5919	22034-22036	VBZ	denotes	is
T5920	22037-22041	CC	denotes	both
T5921	22042-22049	JJ	denotes	nuclear
T5922	22050-22053	CC	denotes	and
T5923	22054-22065	JJ	denotes	cytoplasmic
T5924	22066-22067	-LRB-	denotes	[
T5925	22067-22069	CD	denotes	38
T5926	22069-22070	-RRB-	denotes	]
T5927	22070-22071	.	denotes	.
T5928	22071-22207	sentence	denotes	Therefore cytoplasmic PAX6 could potentially interact with all three proteins, while nuclear PAX6 could interact with TRIM11 and DNCL1.
T5929	22072-22081	RB	denotes	Therefore
T5931	22082-22093	JJ	denotes	cytoplasmic
T5932	22094-22098	NN	denotes	PAX6
T5933	22099-22104	MD	denotes	could
T5934	22105-22116	RB	denotes	potentially
T5930	22117-22125	VB	denotes	interact
T5935	22126-22130	IN	denotes	with
T5936	22131-22134	DT	denotes	all
T5938	22135-22140	CD	denotes	three
T5937	22141-22149	NN	denotes	proteins
T5939	22149-22151	,	denotes	,
T5940	22151-22156	IN	denotes	while
T5942	22157-22164	JJ	denotes	nuclear
T5943	22165-22169	NN	denotes	PAX6
T5944	22170-22175	MD	denotes	could
T5941	22176-22184	VB	denotes	interact
T5945	22185-22189	IN	denotes	with
T5946	22190-22196	NN	denotes	TRIM11
T5947	22197-22200	CC	denotes	and
T5948	22201-22206	NN	denotes	DNCL1
T5949	22206-22207	.	denotes	.
T5950	22207-22392	sentence	denotes	At the tissue level, HOMER3 expression has been detected in thymus and lung but it has mainly been studied in brain where it is found in the forebrain, hippocampus and cerebellum [39].
T5951	22208-22210	IN	denotes	At
T5953	22211-22214	DT	denotes	the
T5955	22215-22221	NN	denotes	tissue
T5954	22222-22227	NN	denotes	level
T5956	22227-22229	,	denotes	,
T5957	22229-22235	NN	denotes	HOMER3
T5958	22236-22246	NN	denotes	expression
T5959	22247-22250	VBZ	denotes	has
T5960	22251-22255	VBN	denotes	been
T5952	22256-22264	VBN	denotes	detected
T5961	22265-22267	IN	denotes	in
T5962	22268-22274	NN	denotes	thymus
T5963	22275-22278	CC	denotes	and
T5964	22279-22283	NN	denotes	lung
T5965	22284-22287	CC	denotes	but
T5966	22288-22290	PRP	denotes	it
T5968	22291-22294	VBZ	denotes	has
T5969	22295-22301	RB	denotes	mainly
T5970	22302-22306	VBN	denotes	been
T5967	22307-22314	VBN	denotes	studied
T5971	22315-22317	IN	denotes	in
T5972	22318-22323	NN	denotes	brain
T5973	22324-22329	WRB	denotes	where
T5975	22330-22332	PRP	denotes	it
T5976	22333-22335	VBZ	denotes	is
T5974	22336-22341	VBN	denotes	found
T5977	22342-22344	IN	denotes	in
T5978	22345-22348	DT	denotes	the
T5979	22349-22358	NN	denotes	forebrain
T5980	22358-22360	,	denotes	,
T5981	22360-22371	NN	denotes	hippocampus
T5982	22372-22375	CC	denotes	and
T5983	22376-22386	NN	denotes	cerebellum
T5984	22387-22388	-LRB-	denotes	[
T5985	22388-22390	CD	denotes	39
T5986	22390-22391	-RRB-	denotes	]
T5987	22391-22392	.	denotes	.
T5988	22392-22475	sentence	denotes	DNCL1 and TRIM11 both have wide expression domains that include the brain [44,38].
T5989	22393-22398	NN	denotes	DNCL1
T5991	22399-22402	CC	denotes	and
T5992	22403-22409	NN	denotes	TRIM11
T5993	22410-22414	DT	denotes	both
T5990	22415-22419	VBP	denotes	have
T5994	22420-22424	JJ	denotes	wide
T5996	22425-22435	NN	denotes	expression
T5995	22436-22443	NNS	denotes	domains
T5997	22444-22448	WDT	denotes	that
T5998	22449-22456	VBP	denotes	include
T5999	22457-22460	DT	denotes	the
T6000	22461-22466	NN	denotes	brain
T6001	22467-22468	-LRB-	denotes	[
T6003	22468-22470	CD	denotes	44
T6004	22470-22471	,	denotes	,
T6002	22471-22473	CD	denotes	38
T6005	22473-22474	-RRB-	denotes	]
T6006	22474-22475	.	denotes	.
T6007	22475-22571	sentence	denotes	Thus the expression of all three interactors overlaps with PAX6 at the tissue level [2,7,8,10].
T6008	22476-22480	RB	denotes	Thus
T6010	22481-22484	DT	denotes	the
T6011	22485-22495	NN	denotes	expression
T6012	22496-22498	IN	denotes	of
T6013	22499-22502	DT	denotes	all
T6015	22503-22508	CD	denotes	three
T6014	22509-22520	NNS	denotes	interactors
T6009	22521-22529	VBZ	denotes	overlaps
T6016	22530-22534	IN	denotes	with
T6017	22535-22539	NN	denotes	PAX6
T6018	22540-22542	IN	denotes	at
T6019	22543-22546	DT	denotes	the
T6021	22547-22553	NN	denotes	tissue
T6020	22554-22559	NN	denotes	level
T6022	22560-22561	-LRB-	denotes	[
T6024	22561-22562	CD	denotes	2
T6025	22562-22563	,	denotes	,
T6026	22563-22564	CD	denotes	7
T6027	22564-22565	,	denotes	,
T6028	22565-22566	CD	denotes	8
T6029	22566-22567	,	denotes	,
T6023	22567-22569	CD	denotes	10
T6030	22569-22570	-RRB-	denotes	]
T6031	22570-22571	.	denotes	.
T6032	22571-22712	sentence	denotes	We detected co-expression of PAX6, HOMER3, DNCL1 and TRIM11 by RT-PCR in human adult brain RNA (IH, L Harrison and A Brown, data not shown).
T6033	22572-22574	PRP	denotes	We
T6034	22575-22583	VBD	denotes	detected
T6035	22584-22597	NN	denotes	co-expression
T6036	22598-22600	IN	denotes	of
T6037	22601-22605	NN	denotes	PAX6
T6038	22605-22607	,	denotes	,
T6039	22607-22613	NN	denotes	HOMER3
T6040	22613-22615	,	denotes	,
T6041	22615-22620	NN	denotes	DNCL1
T6042	22621-22624	CC	denotes	and
T6043	22625-22631	NN	denotes	TRIM11
T6044	22632-22634	IN	denotes	by
T6045	22635-22637	NN	denotes	RT
T6047	22637-22638	HYPH	denotes	-
T6046	22638-22641	NN	denotes	PCR
T6048	22642-22644	IN	denotes	in
T6049	22645-22650	JJ	denotes	human
T6051	22651-22656	JJ	denotes	adult
T6052	22657-22662	NN	denotes	brain
T6050	22663-22666	NN	denotes	RNA
T6053	22667-22668	-LRB-	denotes	(
T6054	22668-22670	NNP	denotes	IH
T6055	22670-22672	,	denotes	,
T6056	22672-22673	NNP	denotes	L
T6057	22674-22682	NNP	denotes	Harrison
T6058	22683-22686	CC	denotes	and
T6059	22687-22688	NNP	denotes	A
T6060	22689-22694	NNP	denotes	Brown
T6061	22694-22696	,	denotes	,
T6062	22696-22700	NNS	denotes	data
T6064	22701-22704	RB	denotes	not
T6063	22705-22710	VBN	denotes	shown
T6065	22710-22711	-RRB-	denotes	)
T6066	22711-22712	.	denotes	.
T6067	22712-22893	sentence	denotes	We demonstrated that the interaction between the PST domain and HOMER3 or DNCL1 was impaired by three naturally occurring mutations that are located in the PAX6 C-terminal peptide.
T6068	22713-22715	PRP	denotes	We
T6069	22716-22728	VBD	denotes	demonstrated
T6070	22729-22733	IN	denotes	that
T6072	22734-22737	DT	denotes	the
T6073	22738-22749	NN	denotes	interaction
T6074	22750-22757	IN	denotes	between
T6075	22758-22761	DT	denotes	the
T6077	22762-22765	NN	denotes	PST
T6076	22766-22772	NN	denotes	domain
T6078	22773-22776	CC	denotes	and
T6079	22777-22783	NN	denotes	HOMER3
T6080	22784-22786	CC	denotes	or
T6081	22787-22792	NN	denotes	DNCL1
T6082	22793-22796	VBD	denotes	was
T6071	22797-22805	VBN	denotes	impaired
T6083	22806-22808	IN	denotes	by
T6084	22809-22814	CD	denotes	three
T6086	22815-22824	RB	denotes	naturally
T6087	22825-22834	VBG	denotes	occurring
T6085	22835-22844	NNS	denotes	mutations
T6088	22845-22849	WDT	denotes	that
T6090	22850-22853	VBP	denotes	are
T6089	22854-22861	VBN	denotes	located
T6091	22862-22864	IN	denotes	in
T6092	22865-22868	DT	denotes	the
T6094	22869-22873	NN	denotes	PAX6
T6095	22874-22875	NN	denotes	C
T6097	22875-22876	HYPH	denotes	-
T6096	22876-22884	JJ	denotes	terminal
T6093	22885-22892	NN	denotes	peptide
T6098	22892-22893	.	denotes	.
T6099	22893-23067	sentence	denotes	The Q422R mutation, which involves a glutamine to arginine substitution at the last amino acid position of PAX6, caused a reduction in the interaction with HOMER3 and DNCL1.
T6100	22894-22897	DT	denotes	The
T6102	22898-22903	NN	denotes	Q422R
T6101	22904-22912	NN	denotes	mutation
T6104	22912-22914	,	denotes	,
T6105	22914-22919	WDT	denotes	which
T6106	22920-22928	VBZ	denotes	involves
T6107	22929-22930	DT	denotes	a
T6109	22931-22940	NN	denotes	glutamine
T6110	22941-22943	IN	denotes	to
T6111	22944-22952	NN	denotes	arginine
T6108	22953-22965	NN	denotes	substitution
T6112	22966-22968	IN	denotes	at
T6113	22969-22972	DT	denotes	the
T6115	22973-22977	JJ	denotes	last
T6116	22978-22983	NN	denotes	amino
T6117	22984-22988	NN	denotes	acid
T6114	22989-22997	NN	denotes	position
T6118	22998-23000	IN	denotes	of
T6119	23001-23005	NN	denotes	PAX6
T6120	23005-23007	,	denotes	,
T6103	23007-23013	VBD	denotes	caused
T6121	23014-23015	DT	denotes	a
T6122	23016-23025	NN	denotes	reduction
T6123	23026-23028	IN	denotes	in
T6124	23029-23032	DT	denotes	the
T6125	23033-23044	NN	denotes	interaction
T6126	23045-23049	IN	denotes	with
T6127	23050-23056	NN	denotes	HOMER3
T6128	23057-23060	CC	denotes	and
T6129	23061-23066	NN	denotes	DNCL1
T6130	23066-23067	.	denotes	.
T6131	23067-23181	sentence	denotes	The X423L and 1615del10 mutations severely reduced or completely abolished the interaction with HOMER3 and DNCL1.
T6132	23068-23071	DT	denotes	The
T6134	23072-23077	NN	denotes	X423L
T6135	23078-23081	CC	denotes	and
T6136	23082-23091	NN	denotes	1615del10
T6133	23092-23101	NNS	denotes	mutations
T6138	23102-23110	RB	denotes	severely
T6137	23111-23118	VBN	denotes	reduced
T6139	23119-23121	CC	denotes	or
T6140	23122-23132	RB	denotes	completely
T6141	23133-23142	VBD	denotes	abolished
T6142	23143-23146	DT	denotes	the
T6143	23147-23158	NN	denotes	interaction
T6144	23159-23163	IN	denotes	with
T6145	23164-23170	NN	denotes	HOMER3
T6146	23171-23174	CC	denotes	and
T6147	23175-23180	NN	denotes	DNCL1
T6148	23180-23181	.	denotes	.
T6149	23181-23435	sentence	denotes	The predicted effect of the X423L and 1615del10 mutations is to cause translation into the 3' untranslated region, thus generating proteins with abnormal extensions that might be expected to disrupt the conformation of the C-terminal end of the protein.
T6150	23182-23185	DT	denotes	The
T6152	23186-23195	VBN	denotes	predicted
T6151	23196-23202	NN	denotes	effect
T6154	23203-23205	IN	denotes	of
T6155	23206-23209	DT	denotes	the
T6157	23210-23215	NN	denotes	X423L
T6158	23216-23219	CC	denotes	and
T6159	23220-23229	NN	denotes	1615del10
T6156	23230-23239	NNS	denotes	mutations
T6153	23240-23242	VBZ	denotes	is
T6160	23243-23245	TO	denotes	to
T6161	23246-23251	VB	denotes	cause
T6162	23252-23263	NN	denotes	translation
T6163	23264-23268	IN	denotes	into
T6164	23269-23272	DT	denotes	the
T6166	23273-23274	CD	denotes	3
T6167	23274-23275	SYM	denotes	'
T6168	23276-23288	JJ	denotes	untranslated
T6165	23289-23295	NN	denotes	region
T6169	23295-23297	,	denotes	,
T6170	23297-23301	RB	denotes	thus
T6171	23302-23312	VBG	denotes	generating
T6172	23313-23321	NN	denotes	proteins
T6173	23322-23326	IN	denotes	with
T6174	23327-23335	JJ	denotes	abnormal
T6175	23336-23346	NNS	denotes	extensions
T6176	23347-23351	WDT	denotes	that
T6178	23352-23357	MD	denotes	might
T6179	23358-23360	VB	denotes	be
T6177	23361-23369	VBN	denotes	expected
T6180	23370-23372	TO	denotes	to
T6181	23373-23380	VB	denotes	disrupt
T6182	23381-23384	DT	denotes	the
T6183	23385-23397	NN	denotes	conformation
T6184	23398-23400	IN	denotes	of
T6185	23401-23404	DT	denotes	the
T6187	23405-23406	NN	denotes	C
T6189	23406-23407	HYPH	denotes	-
T6188	23407-23415	JJ	denotes	terminal
T6186	23416-23419	NN	denotes	end
T6190	23420-23422	IN	denotes	of
T6191	23423-23426	DT	denotes	the
T6192	23427-23434	NN	denotes	protein
T6193	23434-23435	.	denotes	.
T6194	23435-23526	sentence	denotes	The 1615del10 mutation also removes the last 5 amino acids of the C-terminal peptide [28].
T6195	23436-23439	DT	denotes	The
T6197	23440-23449	NN	denotes	1615del10
T6196	23450-23458	NN	denotes	mutation
T6199	23459-23463	RB	denotes	also
T6198	23464-23471	VBZ	denotes	removes
T6200	23472-23475	DT	denotes	the
T6202	23476-23480	JJ	denotes	last
T6203	23481-23482	CD	denotes	5
T6204	23483-23488	NN	denotes	amino
T6201	23489-23494	NNS	denotes	acids
T6205	23495-23497	IN	denotes	of
T6206	23498-23501	DT	denotes	the
T6208	23502-23503	NN	denotes	C
T6210	23503-23504	HYPH	denotes	-
T6209	23504-23512	JJ	denotes	terminal
T6207	23513-23520	NN	denotes	peptide
T6211	23521-23522	-LRB-	denotes	[
T6212	23522-23524	CD	denotes	28
T6213	23524-23525	-RRB-	denotes	]
T6214	23525-23526	.	denotes	.
T6215	23526-23684	sentence	denotes	None of the mutations affected the interaction with TRIM11, suggesting that they do not alter the conformation of the more N-terminal part of the PST domain.
T6216	23527-23531	NN	denotes	None
T6218	23532-23534	IN	denotes	of
T6219	23535-23538	DT	denotes	the
T6220	23539-23548	NNS	denotes	mutations
T6217	23549-23557	VBD	denotes	affected
T6221	23558-23561	DT	denotes	the
T6222	23562-23573	NN	denotes	interaction
T6223	23574-23578	IN	denotes	with
T6224	23579-23585	NN	denotes	TRIM11
T6225	23585-23587	,	denotes	,
T6226	23587-23597	VBG	denotes	suggesting
T6227	23598-23602	IN	denotes	that
T6229	23603-23607	PRP	denotes	they
T6230	23608-23610	VBP	denotes	do
T6231	23611-23614	RB	denotes	not
T6228	23615-23620	VB	denotes	alter
T6232	23621-23624	DT	denotes	the
T6233	23625-23637	NN	denotes	conformation
T6234	23638-23640	IN	denotes	of
T6235	23641-23644	DT	denotes	the
T6237	23645-23649	RBR	denotes	more
T6239	23650-23651	NN	denotes	N
T6240	23651-23652	HYPH	denotes	-
T6238	23652-23660	JJ	denotes	terminal
T6236	23661-23665	NN	denotes	part
T6241	23666-23668	IN	denotes	of
T6242	23669-23672	DT	denotes	the
T6244	23673-23676	NN	denotes	PST
T6243	23677-23683	NN	denotes	domain
T6245	23683-23684	.	denotes	.
T6246	23684-23877	sentence	denotes	All our data are consistent with the hypothesis that TRIM11 does not interact with the C-terminal peptide, but interacts with the PST domain between the homeodomain and the C-terminal peptide.
T6247	23685-23688	PDT	denotes	All
T6249	23689-23692	PRP$	denotes	our
T6248	23693-23697	NNS	denotes	data
T6250	23698-23701	VBP	denotes	are
T6251	23702-23712	JJ	denotes	consistent
T6252	23713-23717	IN	denotes	with
T6253	23718-23721	DT	denotes	the
T6254	23722-23732	NN	denotes	hypothesis
T6255	23733-23737	IN	denotes	that
T6257	23738-23744	NN	denotes	TRIM11
T6258	23745-23749	VBZ	denotes	does
T6259	23750-23753	RB	denotes	not
T6256	23754-23762	VB	denotes	interact
T6260	23763-23767	IN	denotes	with
T6261	23768-23771	DT	denotes	the
T6263	23772-23773	NN	denotes	C
T6265	23773-23774	HYPH	denotes	-
T6264	23774-23782	JJ	denotes	terminal
T6262	23783-23790	NN	denotes	peptide
T6266	23790-23792	,	denotes	,
T6267	23792-23795	CC	denotes	but
T6268	23796-23805	VBZ	denotes	interacts
T6269	23806-23810	IN	denotes	with
T6270	23811-23814	DT	denotes	the
T6272	23815-23818	NN	denotes	PST
T6271	23819-23825	NN	denotes	domain
T6273	23826-23833	IN	denotes	between
T6274	23834-23837	DT	denotes	the
T6275	23838-23849	NN	denotes	homeodomain
T6276	23850-23853	CC	denotes	and
T6277	23854-23857	DT	denotes	the
T6279	23858-23859	NN	denotes	C
T6281	23859-23860	HYPH	denotes	-
T6280	23860-23868	JJ	denotes	terminal
T6278	23869-23876	NN	denotes	peptide
T6282	23876-23877	.	denotes	.
T6283	23877-24017	sentence	denotes	Most aniridia patients are heterozygous for mutations that introduce a premature termination codon into the PAX6 open reading frame [2,52].
T6284	23878-23882	JJS	denotes	Most
T6286	23883-23891	NN	denotes	aniridia
T6285	23892-23900	NNS	denotes	patients
T6287	23901-23904	VBP	denotes	are
T6288	23905-23917	JJ	denotes	heterozygous
T6289	23918-23921	IN	denotes	for
T6290	23922-23931	NNS	denotes	mutations
T6291	23932-23936	WDT	denotes	that
T6292	23937-23946	VBP	denotes	introduce
T6293	23947-23948	DT	denotes	a
T6295	23949-23958	JJ	denotes	premature
T6296	23959-23970	NN	denotes	termination
T6294	23971-23976	NN	denotes	codon
T6297	23977-23981	IN	denotes	into
T6298	23982-23985	DT	denotes	the
T6300	23986-23990	NN	denotes	PAX6
T6301	23991-23995	JJ	denotes	open
T6302	23996-24003	NN	denotes	reading
T6299	24004-24009	NN	denotes	frame
T6303	24010-24011	-LRB-	denotes	[
T6305	24011-24012	CD	denotes	2
T6306	24012-24013	,	denotes	,
T6304	24013-24015	CD	denotes	52
T6307	24015-24016	-RRB-	denotes	]
T6308	24016-24017	.	denotes	.
T6309	24017-24162	sentence	denotes	These alleles would be expected to encode truncated proteins or no protein at all if the mutant RNA is degraded by nonsense-mediated decay [52].
T6310	24018-24023	DT	denotes	These
T6311	24024-24031	NNS	denotes	alleles
T6313	24032-24037	MD	denotes	would
T6314	24038-24040	VB	denotes	be
T6312	24041-24049	VBN	denotes	expected
T6315	24050-24052	TO	denotes	to
T6316	24053-24059	VB	denotes	encode
T6317	24060-24069	VBN	denotes	truncated
T6318	24070-24078	NN	denotes	proteins
T6319	24079-24081	CC	denotes	or
T6320	24082-24084	DT	denotes	no
T6321	24085-24092	NN	denotes	protein
T6322	24093-24095	RB	denotes	at
T6323	24096-24099	RB	denotes	all
T6324	24100-24102	IN	denotes	if
T6326	24103-24106	DT	denotes	the
T6328	24107-24113	NN	denotes	mutant
T6327	24114-24117	NN	denotes	RNA
T6329	24118-24120	VBZ	denotes	is
T6325	24121-24129	VBN	denotes	degraded
T6330	24130-24132	IN	denotes	by
T6331	24133-24141	NN	denotes	nonsense
T6333	24141-24142	HYPH	denotes	-
T6332	24142-24150	VBN	denotes	mediated
T6334	24151-24156	NN	denotes	decay
T6335	24157-24158	-LRB-	denotes	[
T6336	24158-24160	CD	denotes	52
T6337	24160-24161	-RRB-	denotes	]
T6338	24161-24162	.	denotes	.
T6339	24162-24334	sentence	denotes	We propose that the brain anomalies that have been observed in aniridia patients may be partly explained by impaired interaction between PAX6 and HOMER3, DNCL1 and TRIM11.
T6340	24163-24165	PRP	denotes	We
T6341	24166-24173	VBP	denotes	propose
T6342	24174-24178	IN	denotes	that
T6344	24179-24182	DT	denotes	the
T6346	24183-24188	NN	denotes	brain
T6345	24189-24198	NNS	denotes	anomalies
T6347	24199-24203	WDT	denotes	that
T6349	24204-24208	VBP	denotes	have
T6350	24209-24213	VBN	denotes	been
T6348	24214-24222	VBN	denotes	observed
T6351	24223-24225	IN	denotes	in
T6352	24226-24234	NN	denotes	aniridia
T6353	24235-24243	NNS	denotes	patients
T6354	24244-24247	MD	denotes	may
T6355	24248-24250	VB	denotes	be
T6356	24251-24257	RB	denotes	partly
T6343	24258-24267	VBN	denotes	explained
T6357	24268-24270	IN	denotes	by
T6358	24271-24279	VBN	denotes	impaired
T6359	24280-24291	NN	denotes	interaction
T6360	24292-24299	IN	denotes	between
T6361	24300-24304	NN	denotes	PAX6
T6362	24305-24308	CC	denotes	and
T6363	24309-24315	NN	denotes	HOMER3
T6364	24315-24317	,	denotes	,
T6365	24317-24322	NN	denotes	DNCL1
T6366	24323-24326	CC	denotes	and
T6367	24327-24333	NN	denotes	TRIM11
T6368	24333-24334	.	denotes	.
T6369	24334-24519	sentence	denotes	The neurobehavioural phenotype associated with 1615del10 and the polymicrogyria associated with X423L may result from a specific effect of these unusual C-terminal extension mutations.
T6370	24335-24338	DT	denotes	The
T6372	24339-24355	JJ	denotes	neurobehavioural
T6371	24356-24365	NN	denotes	phenotype
T6374	24366-24376	VBN	denotes	associated
T6375	24377-24381	IN	denotes	with
T6376	24382-24391	NN	denotes	1615del10
T6377	24392-24395	CC	denotes	and
T6378	24396-24399	DT	denotes	the
T6379	24400-24414	NN	denotes	polymicrogyria
T6380	24415-24425	VBN	denotes	associated
T6381	24426-24430	IN	denotes	with
T6382	24431-24436	NN	denotes	X423L
T6383	24437-24440	MD	denotes	may
T6373	24441-24447	VB	denotes	result
T6384	24448-24452	IN	denotes	from
T6385	24453-24454	DT	denotes	a
T6387	24455-24463	JJ	denotes	specific
T6386	24464-24470	NN	denotes	effect
T6388	24471-24473	IN	denotes	of
T6389	24474-24479	DT	denotes	these
T6391	24480-24487	JJ	denotes	unusual
T6392	24488-24489	NN	denotes	C
T6394	24489-24490	HYPH	denotes	-
T6393	24490-24498	JJ	denotes	terminal
T6395	24499-24508	NN	denotes	extension
T6390	24509-24518	NNS	denotes	mutations
T6396	24518-24519	.	denotes	.
T6397	24519-24825	sentence	denotes	There is evidence that signalling and transport mechanisms that were initially characterized in the brain may also be conserved in the retina, suggesting that impaired PAX6 protein-protein interactions may also have implications for the retinal defects observed in individuals with PAX6 mutations [53,54].
T6398	24520-24525	EX	denotes	There
T6399	24526-24528	VBZ	denotes	is
T6400	24529-24537	NN	denotes	evidence
T6401	24538-24542	IN	denotes	that
T6403	24543-24553	NN	denotes	signalling
T6405	24554-24557	CC	denotes	and
T6406	24558-24567	NN	denotes	transport
T6404	24568-24578	NNS	denotes	mechanisms
T6407	24579-24583	WDT	denotes	that
T6409	24584-24588	VBD	denotes	were
T6410	24589-24598	RB	denotes	initially
T6408	24599-24612	VBN	denotes	characterized
T6411	24613-24615	IN	denotes	in
T6412	24616-24619	DT	denotes	the
T6413	24620-24625	NN	denotes	brain
T6414	24626-24629	MD	denotes	may
T6415	24630-24634	RB	denotes	also
T6416	24635-24637	VB	denotes	be
T6402	24638-24647	VBN	denotes	conserved
T6417	24648-24650	IN	denotes	in
T6418	24651-24654	DT	denotes	the
T6419	24655-24661	NN	denotes	retina
T6420	24661-24663	,	denotes	,
T6421	24663-24673	VBG	denotes	suggesting
T6422	24674-24678	IN	denotes	that
T6424	24679-24687	VBN	denotes	impaired
T6426	24688-24692	NN	denotes	PAX6
T6427	24693-24700	NN	denotes	protein
T6429	24700-24701	SYM	denotes	-
T6428	24701-24708	NN	denotes	protein
T6425	24709-24721	NNS	denotes	interactions
T6430	24722-24725	MD	denotes	may
T6431	24726-24730	RB	denotes	also
T6423	24731-24735	VB	denotes	have
T6432	24736-24748	NNS	denotes	implications
T6433	24749-24752	IN	denotes	for
T6434	24753-24756	DT	denotes	the
T6436	24757-24764	JJ	denotes	retinal
T6435	24765-24772	NNS	denotes	defects
T6437	24773-24781	VBN	denotes	observed
T6438	24782-24784	IN	denotes	in
T6439	24785-24796	NNS	denotes	individuals
T6440	24797-24801	IN	denotes	with
T6441	24802-24806	NN	denotes	PAX6
T6442	24807-24816	NNS	denotes	mutations
T6443	24817-24818	-LRB-	denotes	[
T6445	24818-24820	CD	denotes	53
T6446	24820-24821	,	denotes	,
T6444	24821-24823	CD	denotes	54
T6447	24823-24824	-RRB-	denotes	]
T6448	24824-24825	.	denotes	.
T6504	24838-24840	PRP	denotes	We
T6506	24841-24845	VBP	denotes	have
T6505	24846-24855	VBN	denotes	presented
T6507	24856-24867	JJ	denotes	preliminary
T6508	24868-24876	NN	denotes	evidence
T6509	24877-24881	IN	denotes	that
T6511	24882-24885	DT	denotes	the
T6513	24886-24904	JJ	denotes	neurodevelopmental
T6514	24905-24920	JJ	denotes	transcriptional
T6512	24921-24930	NN	denotes	regulator
T6515	24931-24935	NN	denotes	PAX6
T6510	24936-24945	VBZ	denotes	interacts
T6516	24946-24950	IN	denotes	with
T6517	24951-24957	NN	denotes	HOMER3
T6518	24957-24959	,	denotes	,
T6519	24959-24964	NN	denotes	DNCL1
T6520	24965-24968	CC	denotes	and
T6521	24969-24975	NN	denotes	TRIM11
T6522	24975-24976	.	denotes	.
T6523	24976-25145	sentence	denotes	We suggest that the interaction of PAX6 with HOMER3 and DNCL1 is a mechanism by which synaptic signalling could lead to regulated changes in gene expression in neurons.
T6524	24977-24979	PRP	denotes	We
T6525	24980-24987	VBP	denotes	suggest
T6526	24988-24992	IN	denotes	that
T6528	24993-24996	DT	denotes	the
T6529	24997-25008	NN	denotes	interaction
T6530	25009-25011	IN	denotes	of
T6531	25012-25016	NN	denotes	PAX6
T6532	25017-25021	IN	denotes	with
T6533	25022-25028	NN	denotes	HOMER3
T6534	25029-25032	CC	denotes	and
T6535	25033-25038	NN	denotes	DNCL1
T6527	25039-25041	VBZ	denotes	is
T6536	25042-25043	DT	denotes	a
T6537	25044-25053	NN	denotes	mechanism
T6538	25054-25056	IN	denotes	by
T6540	25057-25062	WDT	denotes	which
T6541	25063-25071	JJ	denotes	synaptic
T6542	25072-25082	NN	denotes	signalling
T6543	25083-25088	MD	denotes	could
T6539	25089-25093	VB	denotes	lead
T6544	25094-25096	IN	denotes	to
T6545	25097-25106	VBN	denotes	regulated
T6546	25107-25114	NNS	denotes	changes
T6547	25115-25117	IN	denotes	in
T6548	25118-25122	NN	denotes	gene
T6549	25123-25133	NN	denotes	expression
T6550	25134-25136	IN	denotes	in
T6551	25137-25144	NNS	denotes	neurons
T6552	25144-25145	.	denotes	.
T6553	25145-25286	sentence	denotes	We also propose that some of the neural anomalies in patients with PAX6 mutations may be explained by impaired protein-protein interactions.
T6554	25146-25148	PRP	denotes	We
T6556	25149-25153	RB	denotes	also
T6555	25154-25161	VBP	denotes	propose
T6557	25162-25166	IN	denotes	that
T6559	25167-25171	DT	denotes	some
T6560	25172-25174	IN	denotes	of
T6561	25175-25178	DT	denotes	the
T6563	25179-25185	JJ	denotes	neural
T6562	25186-25195	NNS	denotes	anomalies
T6564	25196-25198	IN	denotes	in
T6565	25199-25207	NNS	denotes	patients
T6566	25208-25212	IN	denotes	with
T6567	25213-25217	NN	denotes	PAX6
T6568	25218-25227	NNS	denotes	mutations
T6569	25228-25231	MD	denotes	may
T6570	25232-25234	VB	denotes	be
T6558	25235-25244	VBN	denotes	explained
T6571	25245-25247	IN	denotes	by
T6572	25248-25256	VBN	denotes	impaired
T6574	25257-25264	NN	denotes	protein
T6576	25264-25265	SYM	denotes	-
T6575	25265-25272	NN	denotes	protein
T6573	25273-25285	NNS	denotes	interactions
T6577	25285-25286	.	denotes	.
T6595	25297-25311	NN	denotes	Bioinformatics
T6596	25312-25322	NNS	denotes	techniques
T6597	25322-25500	sentence	denotes	Sequence database searches were carried out using the BLAST program available through the Bioinformatics Applications at the Rosalind Franklin Centre for Genomics Research [24].
T6598	25323-25331	NN	denotes	Sequence
T6600	25332-25340	NN	denotes	database
T6599	25341-25349	NNS	denotes	searches
T6602	25350-25354	VBD	denotes	were
T6601	25355-25362	VBN	denotes	carried
T6603	25363-25366	RP	denotes	out
T6604	25367-25372	VBG	denotes	using
T6605	25373-25376	DT	denotes	the
T6607	25377-25382	NNP	denotes	BLAST
T6606	25383-25390	NN	denotes	program
T6608	25391-25400	JJ	denotes	available
T6609	25401-25408	IN	denotes	through
T6610	25409-25412	DT	denotes	the
T6612	25413-25427	NNP	denotes	Bioinformatics
T6611	25428-25440	NNP	denotes	Applications
T6613	25441-25443	IN	denotes	at
T6614	25444-25447	DT	denotes	the
T6616	25448-25456	NNP	denotes	Rosalind
T6617	25457-25465	NNP	denotes	Franklin
T6615	25466-25472	NNP	denotes	Centre
T6618	25473-25476	IN	denotes	for
T6619	25477-25485	NNP	denotes	Genomics
T6620	25486-25494	NNP	denotes	Research
T6621	25495-25496	-LRB-	denotes	[
T6622	25496-25498	CD	denotes	24
T6623	25498-25499	-RRB-	denotes	]
T6624	25499-25500	.	denotes	.
T6625	25500-25611	sentence	denotes	Protein sequences that were highly homologous to the C-terminus of human PAX6 were aligned using CLUSTAL [24].
T6626	25501-25508	NN	denotes	Protein
T6627	25509-25518	NNS	denotes	sequences
T6629	25519-25523	WDT	denotes	that
T6630	25524-25528	VBD	denotes	were
T6631	25529-25535	RB	denotes	highly
T6632	25536-25546	JJ	denotes	homologous
T6633	25547-25549	IN	denotes	to
T6634	25550-25553	DT	denotes	the
T6636	25554-25555	NN	denotes	C
T6637	25555-25556	HYPH	denotes	-
T6635	25556-25564	NN	denotes	terminus
T6638	25565-25567	IN	denotes	of
T6639	25568-25573	JJ	denotes	human
T6640	25574-25578	NN	denotes	PAX6
T6641	25579-25583	VBD	denotes	were
T6628	25584-25591	VBN	denotes	aligned
T6642	25592-25597	VBG	denotes	using
T6643	25598-25605	NNP	denotes	CLUSTAL
T6644	25606-25607	-LRB-	denotes	[
T6645	25607-25609	CD	denotes	24
T6646	25609-25610	-RRB-	denotes	]
T6647	25610-25611	.	denotes	.
T6648	25611-25698	sentence	denotes	Secondary structure prediction was performed using the JPRED consensus method [25,26].
T6649	25612-25621	JJ	denotes	Secondary
T6650	25622-25631	NN	denotes	structure
T6651	25632-25642	NN	denotes	prediction
T6653	25643-25646	VBD	denotes	was
T6652	25647-25656	VBN	denotes	performed
T6654	25657-25662	VBG	denotes	using
T6655	25663-25666	DT	denotes	the
T6657	25667-25672	NN	denotes	JPRED
T6658	25673-25682	NN	denotes	consensus
T6656	25683-25689	NN	denotes	method
T6659	25690-25691	-LRB-	denotes	[
T6661	25691-25693	CD	denotes	25
T6662	25693-25694	,	denotes	,
T6660	25694-25696	CD	denotes	26
T6663	25696-25697	-RRB-	denotes	]
T6664	25697-25698	.	denotes	.
T6808	25700-25705	NN	denotes	Yeast
T6810	25706-25709	CD	denotes	two
T6812	25709-25710	HYPH	denotes	-
T6811	25710-25716	NN	denotes	hybrid
T6809	25717-25727	NNS	denotes	constructs
T6813	25727-25895	sentence	denotes	All cDNA and amino acid numbering is based on the human PAX6 cDNA and protein reference sequences available from the Human PAX6 Allelic Variant Database web site [27].
T6814	25728-25731	DT	denotes	All
T6816	25732-25736	NN	denotes	cDNA
T6817	25737-25740	CC	denotes	and
T6818	25741-25746	NN	denotes	amino
T6819	25747-25751	NN	denotes	acid
T6815	25752-25761	NN	denotes	numbering
T6820	25762-25764	VBZ	denotes	is
T6821	25765-25770	VBN	denotes	based
T6822	25771-25773	IN	denotes	on
T6823	25774-25777	DT	denotes	the
T6825	25778-25783	JJ	denotes	human
T6827	25784-25788	NN	denotes	PAX6
T6826	25789-25793	NN	denotes	cDNA
T6828	25794-25797	CC	denotes	and
T6829	25798-25805	NN	denotes	protein
T6830	25806-25815	NN	denotes	reference
T6824	25816-25825	NNS	denotes	sequences
T6831	25826-25835	JJ	denotes	available
T6832	25836-25840	IN	denotes	from
T6833	25841-25844	DT	denotes	the
T6835	25845-25850	JJ	denotes	Human
T6836	25851-25855	NN	denotes	PAX6
T6838	25856-25863	JJ	denotes	Allelic
T6839	25864-25871	NN	denotes	Variant
T6837	25872-25880	NN	denotes	Database
T6840	25881-25884	NN	denotes	web
T6834	25885-25889	NN	denotes	site
T6841	25890-25891	-LRB-	denotes	[
T6842	25891-25893	CD	denotes	27
T6843	25893-25894	-RRB-	denotes	]
T6844	25894-25895	.	denotes	.
T6845	25895-26123	sentence	denotes	Standard PCR and subcloning techniques were used to make three PAX6 cDNA constructs in the pDBLeu expression vector (ProQuest Two-Hybrid System, Invitrogen), which generates a protein fused to the yeast GAL4 DNA binding domain.
T6846	25896-25904	JJ	denotes	Standard
T6848	25905-25908	NN	denotes	PCR
T6849	25909-25912	CC	denotes	and
T6850	25913-25923	NN	denotes	subcloning
T6847	25924-25934	NNS	denotes	techniques
T6852	25935-25939	VBD	denotes	were
T6851	25940-25944	VBN	denotes	used
T6853	25945-25947	TO	denotes	to
T6854	25948-25952	VB	denotes	make
T6855	25953-25958	CD	denotes	three
T6857	25959-25963	NN	denotes	PAX6
T6858	25964-25968	NN	denotes	cDNA
T6856	25969-25979	NNS	denotes	constructs
T6859	25980-25982	IN	denotes	in
T6860	25983-25986	DT	denotes	the
T6862	25987-25993	NN	denotes	pDBLeu
T6863	25994-26004	NN	denotes	expression
T6861	26005-26011	NN	denotes	vector
T6864	26012-26013	-LRB-	denotes	(
T6866	26013-26021	NNP	denotes	ProQuest
T6867	26022-26025	CD	denotes	Two
T6869	26025-26026	HYPH	denotes	-
T6868	26026-26032	NN	denotes	Hybrid
T6865	26033-26039	NN	denotes	System
T6870	26039-26041	,	denotes	,
T6871	26041-26051	NNP	denotes	Invitrogen
T6872	26051-26052	-RRB-	denotes	)
T6873	26052-26054	,	denotes	,
T6874	26054-26059	WDT	denotes	which
T6875	26060-26069	VBZ	denotes	generates
T6876	26070-26071	DT	denotes	a
T6877	26072-26079	NN	denotes	protein
T6878	26080-26085	VBN	denotes	fused
T6879	26086-26088	IN	denotes	to
T6880	26089-26092	DT	denotes	the
T6882	26093-26098	NN	denotes	yeast
T6883	26099-26103	NN	denotes	GAL4
T6884	26104-26107	NN	denotes	DNA
T6885	26108-26115	NN	denotes	binding
T6881	26116-26122	NN	denotes	domain
T6886	26122-26123	.	denotes	.
T6887	26123-26216	sentence	denotes	PAX6PST contains the whole PST domain (amino acids 278–422 of the full-length PAX6 protein).
T6888	26124-26131	NN	denotes	PAX6PST
T6889	26132-26140	VBZ	denotes	contains
T6890	26141-26144	DT	denotes	the
T6892	26145-26150	JJ	denotes	whole
T6893	26151-26154	NN	denotes	PST
T6891	26155-26161	NN	denotes	domain
T6894	26162-26163	-LRB-	denotes	(
T6896	26163-26168	NN	denotes	amino
T6897	26169-26174	NNS	denotes	acids
T6895	26175-26178	CD	denotes	278
T6898	26178-26179	SYM	denotes	–
T6899	26179-26182	CD	denotes	422
T6900	26183-26185	IN	denotes	of
T6901	26186-26189	DT	denotes	the
T6903	26190-26194	JJ	denotes	full
T6905	26194-26195	HYPH	denotes	-
T6904	26195-26201	NN	denotes	length
T6906	26202-26206	NN	denotes	PAX6
T6902	26207-26214	NN	denotes	protein
T6907	26214-26215	-RRB-	denotes	)
T6908	26215-26216	.	denotes	.
T6909	26216-26362	sentence	denotes	Primers were ST001 (forward) 5'-AAA AGT TCG ACT GCC AGC AAC ACA CCT AGT C-3' and ST005 (R) 5'-TTT TGC GGC TTT TTA CTG TAA TCT TGG CCA GTA TTG-3'.
T6910	26217-26224	NNS	denotes	Primers
T6911	26225-26229	VBD	denotes	were
T6912	26230-26235	NN	denotes	ST001
T6914	26236-26237	-LRB-	denotes	(
T6915	26237-26244	JJ	denotes	forward
T6916	26244-26245	-RRB-	denotes	)
T6917	26246-26247	CD	denotes	5
T6918	26247-26248	SYM	denotes	'
T6919	26248-26249	HYPH	denotes	-
T6920	26249-26252	NN	denotes	AAA
T6921	26253-26256	NN	denotes	AGT
T6922	26257-26260	NN	denotes	TCG
T6923	26261-26264	NN	denotes	ACT
T6924	26265-26268	NN	denotes	GCC
T6925	26269-26272	NN	denotes	AGC
T6926	26273-26276	NN	denotes	AAC
T6927	26277-26280	NN	denotes	ACA
T6928	26281-26284	NN	denotes	CCT
T6929	26285-26288	NN	denotes	AGT
T6913	26289-26290	NN	denotes	C
T6930	26290-26291	HYPH	denotes	-
T6931	26291-26292	CD	denotes	3
T6932	26292-26293	SYM	denotes	'
T6933	26294-26297	CC	denotes	and
T6934	26298-26303	NN	denotes	ST005
T6936	26304-26305	-LRB-	denotes	(
T6937	26305-26306	NN	denotes	R
T6938	26306-26307	-RRB-	denotes	)
T6939	26308-26309	CD	denotes	5
T6940	26309-26310	SYM	denotes	'
T6941	26310-26311	HYPH	denotes	-
T6942	26311-26314	NN	denotes	TTT
T6943	26315-26318	NN	denotes	TGC
T6944	26319-26322	NN	denotes	GGC
T6945	26323-26326	NN	denotes	TTT
T6946	26327-26330	NN	denotes	TTA
T6947	26331-26334	NN	denotes	CTG
T6948	26335-26338	NN	denotes	TAA
T6949	26339-26342	NN	denotes	TCT
T6950	26343-26346	NN	denotes	TGG
T6951	26347-26350	NN	denotes	CCA
T6952	26351-26354	NN	denotes	GTA
T6935	26355-26358	NN	denotes	TTG
T6953	26358-26359	HYPH	denotes	-
T6954	26359-26360	CD	denotes	3
T6955	26360-26361	SYM	denotes	'
T6956	26361-26362	.	denotes	.
T6957	26362-26433	sentence	denotes	PAX6CTP contains the newly defined C-terminal peptide alone (391–422).
T6958	26363-26370	NN	denotes	PAX6CTP
T6959	26371-26379	VBZ	denotes	contains
T6960	26380-26383	DT	denotes	the
T6962	26384-26389	RB	denotes	newly
T6963	26390-26397	VBN	denotes	defined
T6964	26398-26399	NN	denotes	C
T6966	26399-26400	HYPH	denotes	-
T6965	26400-26408	JJ	denotes	terminal
T6961	26409-26416	NN	denotes	peptide
T6967	26417-26422	JJ	denotes	alone
T6968	26423-26424	-LRB-	denotes	(
T6969	26424-26427	CD	denotes	391
T6970	26427-26428	SYM	denotes	–
T6971	26428-26431	CD	denotes	422
T6972	26431-26432	-RRB-	denotes	)
T6973	26432-26433	.	denotes	.
T6974	26433-26520	sentence	denotes	Primers were ST004 (F) 5'-AAA AGT CGA CTA CCA CTT CAA CAG GAC TCA TT-3' and ST005 (R).
T6975	26434-26441	NNS	denotes	Primers
T6976	26442-26446	VBD	denotes	were
T6977	26447-26452	NN	denotes	ST004
T6979	26453-26454	-LRB-	denotes	(
T6980	26454-26455	NN	denotes	F
T6981	26455-26456	-RRB-	denotes	)
T6982	26457-26458	CD	denotes	5
T6983	26458-26459	SYM	denotes	'
T6984	26459-26460	HYPH	denotes	-
T6985	26460-26463	NN	denotes	AAA
T6986	26464-26467	NN	denotes	AGT
T6987	26468-26471	NN	denotes	CGA
T6988	26472-26475	NN	denotes	CTA
T6989	26476-26479	NN	denotes	CCA
T6990	26480-26483	NN	denotes	CTT
T6991	26484-26487	NN	denotes	CAA
T6992	26488-26491	NN	denotes	CAG
T6993	26492-26495	NN	denotes	GAC
T6994	26496-26499	NN	denotes	TCA
T6978	26500-26502	NN	denotes	TT
T6995	26502-26503	HYPH	denotes	-
T6996	26503-26504	CD	denotes	3
T6997	26504-26505	SYM	denotes	'
T6998	26506-26509	CC	denotes	and
T6999	26510-26515	NN	denotes	ST005
T7000	26516-26517	-LRB-	denotes	(
T7001	26517-26518	NN	denotes	R
T7002	26518-26519	-RRB-	denotes	)
T7003	26519-26520	.	denotes	.
T7004	26520-26595	sentence	denotes	PAX6PST-CT contains the PST domain minus the C-terminal peptide (278–390).
T7005	26521-26528	NN	denotes	PAX6PST
T7007	26528-26529	HYPH	denotes	-
T7006	26529-26531	NN	denotes	CT
T7008	26532-26540	VBZ	denotes	contains
T7009	26541-26544	DT	denotes	the
T7011	26545-26548	NN	denotes	PST
T7010	26549-26555	NN	denotes	domain
T7012	26556-26561	CC	denotes	minus
T7013	26562-26565	DT	denotes	the
T7015	26566-26567	NN	denotes	C
T7017	26567-26568	HYPH	denotes	-
T7016	26568-26576	JJ	denotes	terminal
T7014	26577-26584	NN	denotes	peptide
T7018	26585-26586	-LRB-	denotes	(
T7019	26586-26589	CD	denotes	278
T7020	26589-26590	SYM	denotes	–
T7021	26590-26593	CD	denotes	390
T7022	26593-26594	-RRB-	denotes	)
T7023	26594-26595	.	denotes	.
T7024	26595-26751	sentence	denotes	This was made by cutting PAX6PST with NdeI and NotI to drop out the C-terminal peptide, and inserting a synthetic linker between the two restriction sites.
T7025	26596-26600	DT	denotes	This
T7027	26601-26604	VBD	denotes	was
T7026	26605-26609	VBN	denotes	made
T7028	26610-26612	IN	denotes	by
T7029	26613-26620	VBG	denotes	cutting
T7030	26621-26628	NN	denotes	PAX6PST
T7031	26629-26633	IN	denotes	with
T7032	26634-26638	NN	denotes	NdeI
T7033	26639-26642	CC	denotes	and
T7034	26643-26647	NN	denotes	NotI
T7035	26648-26650	TO	denotes	to
T7036	26651-26655	VB	denotes	drop
T7037	26656-26659	RP	denotes	out
T7038	26660-26663	DT	denotes	the
T7040	26664-26665	NN	denotes	C
T7042	26665-26666	HYPH	denotes	-
T7041	26666-26674	JJ	denotes	terminal
T7039	26675-26682	NN	denotes	peptide
T7043	26682-26684	,	denotes	,
T7044	26684-26687	CC	denotes	and
T7045	26688-26697	VBG	denotes	inserting
T7046	26698-26699	DT	denotes	a
T7048	26700-26709	JJ	denotes	synthetic
T7047	26710-26716	NN	denotes	linker
T7049	26717-26724	IN	denotes	between
T7050	26725-26728	DT	denotes	the
T7052	26729-26732	CD	denotes	two
T7053	26733-26744	NN	denotes	restriction
T7051	26745-26750	NNS	denotes	sites
T7054	26750-26751	.	denotes	.
T7055	26751-26858	sentence	denotes	All fragments generated by PCR or with linkers were sequenced to check that no errors had been introduced.
T7056	26752-26755	DT	denotes	All
T7057	26756-26765	NNS	denotes	fragments
T7059	26766-26775	VBN	denotes	generated
T7060	26776-26778	IN	denotes	by
T7061	26779-26782	NN	denotes	PCR
T7062	26783-26785	CC	denotes	or
T7063	26786-26790	IN	denotes	with
T7064	26791-26798	NNS	denotes	linkers
T7065	26799-26803	VBD	denotes	were
T7058	26804-26813	VBN	denotes	sequenced
T7066	26814-26816	TO	denotes	to
T7067	26817-26822	VB	denotes	check
T7068	26823-26827	IN	denotes	that
T7070	26828-26830	DT	denotes	no
T7071	26831-26837	NNS	denotes	errors
T7072	26838-26841	VBD	denotes	had
T7073	26842-26846	VBN	denotes	been
T7069	26847-26857	VBN	denotes	introduced
T7074	26857-26858	.	denotes	.
T7075	26858-27147	sentence	denotes	A PAX6 PST domain construct containing the mutation 1627A>G (Q422R) was generated in the same way as the PAX6PST construct, but using the reverse PCR primer ST006 5'-TTT TGC GGC CGC TTT TTA CCG TAA TCT TGG CCA GTA TTG AG-3', which contains the mutant nucleotide substitution (underlined).
T7076	26859-26860	DT	denotes	A
T7078	26861-26865	NN	denotes	PAX6
T7080	26866-26869	NN	denotes	PST
T7079	26870-26876	NN	denotes	domain
T7077	26877-26886	NN	denotes	construct
T7082	26887-26897	VBG	denotes	containing
T7083	26898-26901	DT	denotes	the
T7084	26902-26910	NN	denotes	mutation
T7085	26911-26916	NN	denotes	1627A
T7086	26916-26917	SYM	denotes	>
T7087	26917-26918	NN	denotes	G
T7088	26919-26920	-LRB-	denotes	(
T7089	26920-26925	NN	denotes	Q422R
T7090	26925-26926	-RRB-	denotes	)
T7091	26927-26930	VBD	denotes	was
T7081	26931-26940	VBN	denotes	generated
T7092	26941-26943	IN	denotes	in
T7093	26944-26947	DT	denotes	the
T7095	26948-26952	JJ	denotes	same
T7094	26953-26956	NN	denotes	way
T7096	26957-26959	IN	denotes	as
T7097	26960-26963	DT	denotes	the
T7099	26964-26971	NN	denotes	PAX6PST
T7098	26972-26981	NN	denotes	construct
T7100	26981-26983	,	denotes	,
T7101	26983-26986	CC	denotes	but
T7102	26987-26992	VBG	denotes	using
T7103	26993-26996	DT	denotes	the
T7105	26997-27004	JJ	denotes	reverse
T7106	27005-27008	NN	denotes	PCR
T7104	27009-27015	NN	denotes	primer
T7107	27016-27021	NN	denotes	ST006
T7109	27022-27023	CD	denotes	5
T7110	27023-27024	SYM	denotes	'
T7111	27024-27025	HYPH	denotes	-
T7112	27025-27028	NN	denotes	TTT
T7113	27029-27032	NN	denotes	TGC
T7114	27033-27036	NN	denotes	GGC
T7115	27037-27040	NN	denotes	CGC
T7116	27041-27044	NN	denotes	TTT
T7117	27045-27048	NN	denotes	TTA
T7118	27049-27052	NN	denotes	CCG
T7119	27053-27056	NN	denotes	TAA
T7120	27057-27060	NN	denotes	TCT
T7121	27061-27064	NN	denotes	TGG
T7122	27065-27068	NN	denotes	CCA
T7123	27069-27072	NN	denotes	GTA
T7124	27073-27076	NN	denotes	TTG
T7108	27077-27079	NN	denotes	AG
T7125	27079-27080	HYPH	denotes	-
T7126	27080-27081	CD	denotes	3
T7127	27081-27082	SYM	denotes	'
T7128	27082-27084	,	denotes	,
T7129	27084-27089	WDT	denotes	which
T7130	27090-27098	VBZ	denotes	contains
T7131	27099-27102	DT	denotes	the
T7133	27103-27109	NN	denotes	mutant
T7134	27110-27120	NN	denotes	nucleotide
T7132	27121-27133	NN	denotes	substitution
T7135	27134-27135	-LRB-	denotes	(
T7136	27135-27145	JJ	denotes	underlined
T7137	27145-27146	-RRB-	denotes	)
T7138	27146-27147	.	denotes	.
T7139	27147-27335	sentence	denotes	cDNA sequences containing the mutations 1615del10 [28] and 1629insT (X423L) [12] were generated by PCR from reverse transcribed RNA (a gift from Dr K Williamson and Prof V van Heyningen).
T7140	27148-27152	NN	denotes	cDNA
T7141	27153-27162	NNS	denotes	sequences
T7143	27163-27173	VBG	denotes	containing
T7144	27174-27177	DT	denotes	the
T7145	27178-27187	NNS	denotes	mutations
T7146	27188-27197	NN	denotes	1615del10
T7147	27198-27199	-LRB-	denotes	[
T7148	27199-27201	CD	denotes	28
T7149	27201-27202	-RRB-	denotes	]
T7150	27203-27206	CC	denotes	and
T7151	27207-27215	NN	denotes	1629insT
T7152	27216-27217	-LRB-	denotes	(
T7153	27217-27222	NN	denotes	X423L
T7154	27222-27223	-RRB-	denotes	)
T7155	27224-27225	-LRB-	denotes	[
T7156	27225-27227	CD	denotes	12
T7157	27227-27228	-RRB-	denotes	]
T7158	27229-27233	VBD	denotes	were
T7142	27234-27243	VBN	denotes	generated
T7159	27244-27246	IN	denotes	by
T7160	27247-27250	NN	denotes	PCR
T7161	27251-27255	IN	denotes	from
T7162	27256-27263	JJ	denotes	reverse
T7163	27264-27275	VBN	denotes	transcribed
T7164	27276-27279	NN	denotes	RNA
T7165	27280-27281	-LRB-	denotes	(
T7167	27281-27282	DT	denotes	a
T7166	27283-27287	NN	denotes	gift
T7168	27288-27292	IN	denotes	from
T7169	27293-27295	NNP	denotes	Dr
T7171	27296-27297	NNP	denotes	K
T7170	27298-27308	NNP	denotes	Williamson
T7172	27309-27312	CC	denotes	and
T7173	27313-27317	NNP	denotes	Prof
T7175	27318-27319	NNP	denotes	V
T7176	27320-27323	NNP	denotes	van
T7174	27324-27333	NNP	denotes	Heyningen
T7177	27333-27334	-RRB-	denotes	)
T7178	27334-27335	.	denotes	.
T7179	27335-27454	sentence	denotes	Primers were ST015 (F) 5'-CCC ACA TAT GCA GAC ACA C-3' and ST031 (R) 5'-TTG CGG CCG CAT CCA TCC AGT CTA CAT TGT TC-3'.
T7180	27336-27343	NNS	denotes	Primers
T7181	27344-27348	VBD	denotes	were
T7182	27349-27354	NN	denotes	ST015
T7184	27355-27356	-LRB-	denotes	(
T7185	27356-27357	NN	denotes	F
T7186	27357-27358	-RRB-	denotes	)
T7187	27359-27360	CD	denotes	5
T7188	27360-27361	SYM	denotes	'
T7189	27361-27362	HYPH	denotes	-
T7190	27362-27365	NN	denotes	CCC
T7191	27366-27369	NN	denotes	ACA
T7192	27370-27373	NN	denotes	TAT
T7193	27374-27377	NN	denotes	GCA
T7194	27378-27381	NN	denotes	GAC
T7195	27382-27385	NN	denotes	ACA
T7183	27386-27387	NN	denotes	C
T7196	27387-27388	HYPH	denotes	-
T7197	27388-27389	CD	denotes	3
T7198	27389-27390	SYM	denotes	'
T7199	27391-27394	CC	denotes	and
T7200	27395-27400	NN	denotes	ST031
T7202	27401-27402	-LRB-	denotes	(
T7203	27402-27403	NN	denotes	R
T7204	27403-27404	-RRB-	denotes	)
T7205	27405-27406	CD	denotes	5
T7206	27406-27407	SYM	denotes	'
T7207	27407-27408	HYPH	denotes	-
T7208	27408-27411	NN	denotes	TTG
T7209	27412-27415	NN	denotes	CGG
T7210	27416-27419	NN	denotes	CCG
T7211	27420-27423	NN	denotes	CAT
T7212	27424-27427	NN	denotes	CCA
T7213	27428-27431	NN	denotes	TCC
T7214	27432-27435	NN	denotes	AGT
T7215	27436-27439	NN	denotes	CTA
T7216	27440-27443	NN	denotes	CAT
T7217	27444-27447	NN	denotes	TGT
T7201	27448-27450	NN	denotes	TC
T7218	27450-27451	HYPH	denotes	-
T7219	27451-27452	CD	denotes	3
T7220	27452-27453	SYM	denotes	'
T7221	27453-27454	.	denotes	.
T7222	27454-27583	sentence	denotes	The PAX6PST construct was cut with NdeI and NotI to release the normal C-terminal peptide, and the mutant sequence was inserted.
T7223	27455-27458	DT	denotes	The
T7225	27459-27466	NN	denotes	PAX6PST
T7224	27467-27476	NN	denotes	construct
T7227	27477-27480	VBD	denotes	was
T7226	27481-27484	VBN	denotes	cut
T7228	27485-27489	IN	denotes	with
T7229	27490-27494	NN	denotes	NdeI
T7230	27495-27498	CC	denotes	and
T7231	27499-27503	NN	denotes	NotI
T7232	27504-27506	TO	denotes	to
T7233	27507-27514	VB	denotes	release
T7234	27515-27518	DT	denotes	the
T7236	27519-27525	JJ	denotes	normal
T7237	27526-27527	NN	denotes	C
T7239	27527-27528	HYPH	denotes	-
T7238	27528-27536	JJ	denotes	terminal
T7235	27537-27544	NN	denotes	peptide
T7240	27544-27546	,	denotes	,
T7241	27546-27549	CC	denotes	and
T7242	27550-27553	DT	denotes	the
T7244	27554-27560	NN	denotes	mutant
T7243	27561-27569	NN	denotes	sequence
T7246	27570-27573	VBD	denotes	was
T7245	27574-27582	VBN	denotes	inserted
T7247	27582-27583	.	denotes	.
T7368	27585-27590	NN	denotes	Yeast
T7370	27591-27594	CD	denotes	two
T7372	27594-27595	HYPH	denotes	-
T7371	27595-27601	NN	denotes	hybrid
T7373	27602-27609	NN	denotes	library
T7369	27610-27617	NNS	denotes	screens
T7374	27617-27728	sentence	denotes	A mouse brain cDNA library (ProQuest, Invitrogen) was screened with the PAX6PST and PAX6CTP pDBLeu constructs.
T7375	27618-27619	DT	denotes	A
T7377	27620-27625	NN	denotes	mouse
T7378	27626-27631	NN	denotes	brain
T7379	27632-27636	NN	denotes	cDNA
T7376	27637-27644	NN	denotes	library
T7381	27645-27646	-LRB-	denotes	(
T7382	27646-27654	NNP	denotes	ProQuest
T7383	27654-27656	,	denotes	,
T7384	27656-27666	NNP	denotes	Invitrogen
T7385	27666-27667	-RRB-	denotes	)
T7386	27668-27671	VBD	denotes	was
T7380	27672-27680	VBN	denotes	screened
T7387	27681-27685	IN	denotes	with
T7388	27686-27689	DT	denotes	the
T7390	27690-27697	NN	denotes	PAX6PST
T7391	27698-27701	CC	denotes	and
T7392	27702-27709	NN	denotes	PAX6CTP
T7393	27710-27716	NN	denotes	pDBLeu
T7389	27717-27727	NNS	denotes	constructs
T7394	27727-27728	.	denotes	.
T7395	27728-27844	sentence	denotes	The library was constructed in the pPC86 vector, which produces proteins fused to the yeast GAL4 activation domain.
T7396	27729-27732	DT	denotes	The
T7397	27733-27740	NN	denotes	library
T7399	27741-27744	VBD	denotes	was
T7398	27745-27756	VBN	denotes	constructed
T7400	27757-27759	IN	denotes	in
T7401	27760-27763	DT	denotes	the
T7403	27764-27769	NN	denotes	pPC86
T7402	27770-27776	NN	denotes	vector
T7404	27776-27778	,	denotes	,
T7405	27778-27783	WDT	denotes	which
T7406	27784-27792	VBZ	denotes	produces
T7407	27793-27801	NN	denotes	proteins
T7408	27802-27807	VBN	denotes	fused
T7409	27808-27810	IN	denotes	to
T7410	27811-27814	DT	denotes	the
T7412	27815-27820	NN	denotes	yeast
T7413	27821-27825	NN	denotes	GAL4
T7414	27826-27836	NN	denotes	activation
T7411	27837-27843	NN	denotes	domain
T7415	27843-27844	.	denotes	.
T7416	27844-27953	sentence	denotes	The system uses three GAL4-activated reporter genes, HIS3, URA3 and lacZ, to identify positive interactions.
T7417	27845-27848	DT	denotes	The
T7418	27849-27855	NN	denotes	system
T7419	27856-27860	VBZ	denotes	uses
T7420	27861-27866	CD	denotes	three
T7422	27867-27871	NN	denotes	GAL4
T7424	27871-27872	HYPH	denotes	-
T7423	27872-27881	VBN	denotes	activated
T7425	27882-27890	NN	denotes	reporter
T7421	27891-27896	NNS	denotes	genes
T7426	27896-27898	,	denotes	,
T7427	27898-27902	NN	denotes	HIS3
T7428	27902-27904	,	denotes	,
T7429	27904-27908	NN	denotes	URA3
T7430	27909-27912	CC	denotes	and
T7431	27913-27917	NN	denotes	lacZ
T7432	27917-27919	,	denotes	,
T7433	27919-27921	TO	denotes	to
T7434	27922-27930	VB	denotes	identify
T7435	27931-27939	JJ	denotes	positive
T7436	27940-27952	NNS	denotes	interactions
T7437	27952-27953	.	denotes	.
T7438	27953-28157	sentence	denotes	Reporters are activated when the bait protein fused to the GAL4 DNA binding domain (pDBLeu) interacts with the prey protein fused to the GAL4 activation domain (pPC86), thus reconstituting GAL4 function.
T7439	27954-27963	NNS	denotes	Reporters
T7441	27964-27967	VBP	denotes	are
T7440	27968-27977	VBN	denotes	activated
T7442	27978-27982	WRB	denotes	when
T7444	27983-27986	DT	denotes	the
T7446	27987-27991	NN	denotes	bait
T7445	27992-27999	NN	denotes	protein
T7447	28000-28005	VBN	denotes	fused
T7448	28006-28008	IN	denotes	to
T7449	28009-28012	DT	denotes	the
T7451	28013-28017	NN	denotes	GAL4
T7452	28018-28021	NN	denotes	DNA
T7453	28022-28029	NN	denotes	binding
T7450	28030-28036	NN	denotes	domain
T7454	28037-28038	-LRB-	denotes	(
T7455	28038-28044	NN	denotes	pDBLeu
T7456	28044-28045	-RRB-	denotes	)
T7443	28046-28055	VBZ	denotes	interacts
T7457	28056-28060	IN	denotes	with
T7458	28061-28064	DT	denotes	the
T7460	28065-28069	NN	denotes	prey
T7459	28070-28077	NN	denotes	protein
T7461	28078-28083	VBN	denotes	fused
T7462	28084-28086	IN	denotes	to
T7463	28087-28090	DT	denotes	the
T7465	28091-28095	NN	denotes	GAL4
T7466	28096-28106	NN	denotes	activation
T7464	28107-28113	NN	denotes	domain
T7467	28114-28115	-LRB-	denotes	(
T7468	28115-28120	NN	denotes	pPC86
T7469	28120-28121	-RRB-	denotes	)
T7470	28121-28123	,	denotes	,
T7471	28123-28127	RB	denotes	thus
T7472	28128-28142	VBG	denotes	reconstituting
T7473	28143-28147	NN	denotes	GAL4
T7474	28148-28156	NN	denotes	function
T7475	28156-28157	.	denotes	.
T7476	28157-28216	sentence	denotes	HIS3 activation allows growth on plates lacking histidine.
T7477	28158-28162	NN	denotes	HIS3
T7478	28163-28173	NN	denotes	activation
T7479	28174-28180	VBZ	denotes	allows
T7480	28181-28187	NN	denotes	growth
T7481	28188-28190	IN	denotes	on
T7482	28191-28197	NNS	denotes	plates
T7483	28198-28205	VBG	denotes	lacking
T7484	28206-28215	NN	denotes	histidine
T7485	28215-28216	.	denotes	.
T7486	28216-28366	sentence	denotes	Weak URA3 activation suppresses growth on plates containing 5-fluro-orotic acid while strong URA3 activation permits growth on plates lacking uracil.
T7487	28217-28221	JJ	denotes	Weak
T7489	28222-28226	NN	denotes	URA3
T7488	28227-28237	NN	denotes	activation
T7490	28238-28248	VBZ	denotes	suppresses
T7491	28249-28255	NN	denotes	growth
T7492	28256-28258	IN	denotes	on
T7493	28259-28265	NNS	denotes	plates
T7494	28266-28276	VBG	denotes	containing
T7495	28277-28278	CD	denotes	5
T7497	28278-28279	HYPH	denotes	-
T7498	28279-28284	JJ	denotes	fluro
T7499	28284-28285	HYPH	denotes	-
T7496	28285-28291	JJ	denotes	orotic
T7500	28292-28296	NN	denotes	acid
T7501	28297-28302	IN	denotes	while
T7503	28303-28309	JJ	denotes	strong
T7505	28310-28314	NN	denotes	URA3
T7504	28315-28325	NN	denotes	activation
T7502	28326-28333	VBZ	denotes	permits
T7506	28334-28340	NN	denotes	growth
T7507	28341-28343	IN	denotes	on
T7508	28344-28350	NNS	denotes	plates
T7509	28351-28358	VBG	denotes	lacking
T7510	28359-28365	NN	denotes	uracil
T7511	28365-28366	.	denotes	.
T7512	28366-28439	sentence	denotes	LacZ activation causes X-gal to turn blue in a beta-galactosidase assay.
T7513	28367-28371	NN	denotes	LacZ
T7514	28372-28382	NN	denotes	activation
T7515	28383-28389	VBZ	denotes	causes
T7516	28390-28391	NN	denotes	X
T7518	28391-28392	HYPH	denotes	-
T7517	28392-28395	NN	denotes	gal
T7520	28396-28398	TO	denotes	to
T7519	28399-28403	VB	denotes	turn
T7521	28404-28408	JJ	denotes	blue
T7522	28409-28411	IN	denotes	in
T7523	28412-28413	DT	denotes	a
T7525	28414-28418	NN	denotes	beta
T7527	28418-28419	HYPH	denotes	-
T7526	28419-28432	NN	denotes	galactosidase
T7524	28433-28438	NN	denotes	assay
T7528	28438-28439	.	denotes	.
T7529	28439-28591	sentence	denotes	All assays were carried out in parallel with the five ProQuest control yeast strains A-E, which range from non-interactor (A) to strong interactor (E).
T7530	28440-28443	DT	denotes	All
T7531	28444-28450	NNS	denotes	assays
T7533	28451-28455	VBD	denotes	were
T7532	28456-28463	VBN	denotes	carried
T7534	28464-28467	RP	denotes	out
T7535	28468-28470	IN	denotes	in
T7536	28471-28479	NN	denotes	parallel
T7537	28480-28484	IN	denotes	with
T7538	28485-28488	DT	denotes	the
T7540	28489-28493	CD	denotes	five
T7541	28494-28502	NNP	denotes	ProQuest
T7542	28503-28510	NN	denotes	control
T7543	28511-28516	NN	denotes	yeast
T7539	28517-28524	NNS	denotes	strains
T7544	28525-28526	NN	denotes	A
T7545	28526-28527	SYM	denotes	-
T7546	28527-28528	NN	denotes	E
T7547	28528-28530	,	denotes	,
T7548	28530-28535	WDT	denotes	which
T7549	28536-28541	VBP	denotes	range
T7550	28542-28546	IN	denotes	from
T7551	28547-28561	NN	denotes	non-interactor
T7552	28562-28563	-LRB-	denotes	(
T7553	28563-28564	NN	denotes	A
T7554	28564-28565	-RRB-	denotes	)
T7555	28566-28568	IN	denotes	to
T7556	28569-28575	JJ	denotes	strong
T7557	28576-28586	NN	denotes	interactor
T7558	28587-28588	-LRB-	denotes	(
T7559	28588-28589	NN	denotes	E
T7560	28589-28590	-RRB-	denotes	)
T7561	28590-28591	.	denotes	.
T7562	28591-28662	sentence	denotes	All procedures were carried out according to the supplier's protocols.
T7563	28592-28595	DT	denotes	All
T7564	28596-28606	NNS	denotes	procedures
T7566	28607-28611	VBD	denotes	were
T7565	28612-28619	VBN	denotes	carried
T7567	28620-28623	RP	denotes	out
T7568	28624-28633	VBG	denotes	according
T7569	28634-28636	IN	denotes	to
T7570	28637-28640	DT	denotes	the
T7571	28641-28649	NN	denotes	supplier
T7573	28649-28651	POS	denotes	's
T7572	28652-28661	NNS	denotes	protocols
T7574	28661-28662	.	denotes	.
T7575	28662-28808	sentence	denotes	Briefly, chemically competent MaV203 yeast cells were co-transformed with the cDNA library and the bait plasmid pDBLeu PAX6CTP or pDBLeu PAX6PST.
T7576	28663-28670	RB	denotes	Briefly
T7578	28670-28672	,	denotes	,
T7579	28672-28682	RB	denotes	chemically
T7580	28683-28692	JJ	denotes	competent
T7582	28693-28699	NN	denotes	MaV203
T7583	28700-28705	NN	denotes	yeast
T7581	28706-28711	NNS	denotes	cells
T7584	28712-28716	VBD	denotes	were
T7577	28717-28731	VBN	denotes	co-transformed
T7585	28732-28736	IN	denotes	with
T7586	28737-28740	DT	denotes	the
T7588	28741-28745	NN	denotes	cDNA
T7587	28746-28753	NN	denotes	library
T7589	28754-28757	CC	denotes	and
T7590	28758-28761	DT	denotes	the
T7592	28762-28766	NN	denotes	bait
T7591	28767-28774	NN	denotes	plasmid
T7593	28775-28781	NN	denotes	pDBLeu
T7594	28782-28789	NN	denotes	PAX6CTP
T7595	28790-28792	CC	denotes	or
T7596	28793-28799	NN	denotes	pDBLeu
T7597	28800-28807	NN	denotes	PAX6PST
T7598	28807-28808	.	denotes	.
T7599	28808-28902	sentence	denotes	Transformants (5 × 107) were plated on medium lacking histidine to check for HIS3 activation.
T7600	28809-28822	NNS	denotes	Transformants
T7602	28823-28824	-LRB-	denotes	(
T7604	28824-28825	CD	denotes	5
T7605	28826-28827	SYM	denotes	×
T7603	28828-28831	CD	denotes	107
T7606	28831-28832	-RRB-	denotes	)
T7607	28833-28837	VBD	denotes	were
T7601	28838-28844	VBN	denotes	plated
T7608	28845-28847	IN	denotes	on
T7609	28848-28854	NN	denotes	medium
T7610	28855-28862	VBG	denotes	lacking
T7611	28863-28872	NN	denotes	histidine
T7612	28873-28875	TO	denotes	to
T7613	28876-28881	VB	denotes	check
T7614	28882-28885	IN	denotes	for
T7615	28886-28890	NN	denotes	HIS3
T7616	28891-28901	NN	denotes	activation
T7617	28901-28902	.	denotes	.
T7618	28902-28956	sentence	denotes	HIS3 positives were then assayed for all 3 reporters.
T7619	28903-28907	NN	denotes	HIS3
T7620	28908-28917	NNS	denotes	positives
T7622	28918-28922	VBD	denotes	were
T7623	28923-28927	RB	denotes	then
T7621	28928-28935	VBN	denotes	assayed
T7624	28936-28939	IN	denotes	for
T7625	28940-28943	DT	denotes	all
T7627	28944-28945	CD	denotes	3
T7626	28946-28955	NNS	denotes	reporters
T7628	28955-28956	.	denotes	.
T7629	28956-29057	sentence	denotes	The pPC86 prey plasmid was isolated from all HIS3/URA3/LacZ positives and the cDNA insert sequenced.
T7630	28957-28960	DT	denotes	The
T7632	28961-28966	NN	denotes	pPC86
T7633	28967-28971	NN	denotes	prey
T7631	28972-28979	NN	denotes	plasmid
T7635	28980-28983	VBD	denotes	was
T7634	28984-28992	VBN	denotes	isolated
T7636	28993-28997	IN	denotes	from
T7637	28998-29001	DT	denotes	all
T7639	29002-29006	NN	denotes	HIS3
T7641	29006-29007	HYPH	denotes	/
T7642	29007-29011	NN	denotes	URA3
T7643	29011-29012	HYPH	denotes	/
T7640	29012-29016	NN	denotes	LacZ
T7638	29017-29026	NNS	denotes	positives
T7644	29027-29030	CC	denotes	and
T7645	29031-29034	DT	denotes	the
T7647	29035-29039	NN	denotes	cDNA
T7646	29040-29046	NN	denotes	insert
T7648	29047-29056	VBN	denotes	sequenced
T7649	29056-29057	.	denotes	.
T7650	29057-29121	sentence	denotes	BLAST searches were performed to identify the cDNA insert [24].
T7651	29058-29063	NNP	denotes	BLAST
T7652	29064-29072	NNS	denotes	searches
T7654	29073-29077	VBD	denotes	were
T7653	29078-29087	VBN	denotes	performed
T7655	29088-29090	TO	denotes	to
T7656	29091-29099	VB	denotes	identify
T7657	29100-29103	DT	denotes	the
T7659	29104-29108	NN	denotes	cDNA
T7658	29109-29115	NN	denotes	insert
T7660	29116-29117	-LRB-	denotes	[
T7661	29117-29119	CD	denotes	24
T7662	29119-29120	-RRB-	denotes	]
T7663	29120-29121	.	denotes	.
T7711	29123-29131	JJ	denotes	Pairwise
T7712	29132-29144	NNS	denotes	interactions
T7713	29144-29391	sentence	denotes	Specific interactions were tested by transforming competent MaV203 yeast cells with one bait construct (in pDBLeu) and one prey construct (in pPC86) and testing the resulting colonies for activation of the HIS3, URA3 and LacZ reporters as before.
T7714	29145-29153	JJ	denotes	Specific
T7715	29154-29166	NNS	denotes	interactions
T7717	29167-29171	VBD	denotes	were
T7716	29172-29178	VBN	denotes	tested
T7718	29179-29181	IN	denotes	by
T7719	29182-29194	VBG	denotes	transforming
T7720	29195-29204	JJ	denotes	competent
T7722	29205-29211	NN	denotes	MaV203
T7723	29212-29217	NN	denotes	yeast
T7721	29218-29223	NNS	denotes	cells
T7724	29224-29228	IN	denotes	with
T7725	29229-29232	CD	denotes	one
T7726	29233-29237	NN	denotes	bait
T7727	29238-29247	NN	denotes	construct
T7728	29248-29249	-LRB-	denotes	(
T7729	29249-29251	IN	denotes	in
T7730	29252-29258	NN	denotes	pDBLeu
T7731	29258-29259	-RRB-	denotes	)
T7732	29260-29263	CC	denotes	and
T7733	29264-29267	CD	denotes	one
T7734	29268-29272	NN	denotes	prey
T7735	29273-29282	NN	denotes	construct
T7736	29283-29284	-LRB-	denotes	(
T7737	29284-29286	IN	denotes	in
T7738	29287-29292	NN	denotes	pPC86
T7739	29292-29293	-RRB-	denotes	)
T7740	29294-29297	CC	denotes	and
T7741	29298-29305	VBG	denotes	testing
T7742	29306-29309	DT	denotes	the
T7744	29310-29319	VBG	denotes	resulting
T7743	29320-29328	NNS	denotes	colonies
T7745	29329-29332	IN	denotes	for
T7746	29333-29343	NN	denotes	activation
T7747	29344-29346	IN	denotes	of
T7748	29347-29350	DT	denotes	the
T7750	29351-29355	NN	denotes	HIS3
T7751	29355-29357	,	denotes	,
T7752	29357-29361	NN	denotes	URA3
T7753	29362-29365	CC	denotes	and
T7754	29366-29370	NN	denotes	LacZ
T7749	29371-29380	NNS	denotes	reporters
T7755	29381-29383	IN	denotes	as
T7756	29384-29390	RB	denotes	before
T7757	29390-29391	.	denotes	.
T7758	29391-29702	sentence	denotes	To create a full-length Homer3 clone for pairwise tests, a cDNA clone (IMAGE clone 3602414, accession number BE569374) containing the missing N-terminal 70 amino acids was identified by a BLAST search of the EST nucleotide sequence database and obtained from the Rosalind Franklin Centre for Genomics Research.
T7759	29392-29394	TO	denotes	To
T7760	29395-29401	VB	denotes	create
T7762	29402-29403	DT	denotes	a
T7764	29404-29408	JJ	denotes	full
T7766	29408-29409	HYPH	denotes	-
T7765	29409-29415	NN	denotes	length
T7767	29416-29422	NN	denotes	Homer3
T7763	29423-29428	NN	denotes	clone
T7768	29429-29432	IN	denotes	for
T7769	29433-29441	JJ	denotes	pairwise
T7770	29442-29447	NNS	denotes	tests
T7771	29447-29449	,	denotes	,
T7772	29449-29450	DT	denotes	a
T7774	29451-29455	NN	denotes	cDNA
T7773	29456-29461	NN	denotes	clone
T7775	29462-29463	-LRB-	denotes	(
T7777	29463-29468	NN	denotes	IMAGE
T7776	29469-29474	NN	denotes	clone
T7778	29475-29482	CD	denotes	3602414
T7779	29482-29484	,	denotes	,
T7780	29484-29493	NN	denotes	accession
T7782	29494-29500	NN	denotes	number
T7781	29501-29509	NN	denotes	BE569374
T7783	29509-29510	-RRB-	denotes	)
T7784	29511-29521	VBG	denotes	containing
T7785	29522-29525	DT	denotes	the
T7787	29526-29533	JJ	denotes	missing
T7788	29534-29535	NN	denotes	N
T7790	29535-29536	HYPH	denotes	-
T7789	29536-29544	JJ	denotes	terminal
T7791	29545-29547	CD	denotes	70
T7792	29548-29553	NN	denotes	amino
T7786	29554-29559	NNS	denotes	acids
T7793	29560-29563	VBD	denotes	was
T7761	29564-29574	VBN	denotes	identified
T7794	29575-29577	IN	denotes	by
T7795	29578-29579	DT	denotes	a
T7797	29580-29585	NNP	denotes	BLAST
T7796	29586-29592	NN	denotes	search
T7798	29593-29595	IN	denotes	of
T7799	29596-29599	DT	denotes	the
T7801	29600-29603	NN	denotes	EST
T7802	29604-29614	NN	denotes	nucleotide
T7803	29615-29623	NN	denotes	sequence
T7800	29624-29632	NN	denotes	database
T7804	29633-29636	CC	denotes	and
T7805	29637-29645	VBN	denotes	obtained
T7806	29646-29650	IN	denotes	from
T7807	29651-29654	DT	denotes	the
T7809	29655-29663	NNP	denotes	Rosalind
T7810	29664-29672	NNP	denotes	Franklin
T7808	29673-29679	NNP	denotes	Centre
T7811	29680-29683	IN	denotes	for
T7812	29684-29692	NNP	denotes	Genomics
T7813	29693-29701	NNP	denotes	Research
T7814	29701-29702	.	denotes	.
T7815	29702-29854	sentence	denotes	The missing fragment was amplified from the IMAGE clone by PCR and inserted into the pPC86-Homer3 plasmid to create a full-length expression construct.
T7816	29703-29706	DT	denotes	The
T7818	29707-29714	JJ	denotes	missing
T7817	29715-29723	NN	denotes	fragment
T7820	29724-29727	VBD	denotes	was
T7819	29728-29737	VBN	denotes	amplified
T7821	29738-29742	IN	denotes	from
T7822	29743-29746	DT	denotes	the
T7824	29747-29752	NN	denotes	IMAGE
T7823	29753-29758	NN	denotes	clone
T7825	29759-29761	IN	denotes	by
T7826	29762-29765	NN	denotes	PCR
T7827	29766-29769	CC	denotes	and
T7828	29770-29778	VBN	denotes	inserted
T7829	29779-29783	IN	denotes	into
T7830	29784-29787	DT	denotes	the
T7832	29788-29793	NN	denotes	pPC86
T7834	29793-29794	HYPH	denotes	-
T7833	29794-29800	NN	denotes	Homer3
T7831	29801-29808	NN	denotes	plasmid
T7835	29809-29811	TO	denotes	to
T7836	29812-29818	VB	denotes	create
T7837	29819-29820	DT	denotes	a
T7839	29821-29825	JJ	denotes	full
T7841	29825-29826	HYPH	denotes	-
T7840	29826-29832	NN	denotes	length
T7842	29833-29843	NN	denotes	expression
T7838	29844-29853	NN	denotes	construct
T7843	29853-29854	.	denotes	.
T7962	29856-29873	JJ	denotes	Semi-quantitative
T7963	29874-29877	NN	denotes	PCR
T7964	29877-30077	sentence	denotes	To check the relative representation of clones in the cDNA library, semi-quantitative PCR was performed on Pax6, Homer3, Dncl1, Trim11 and the constitutively expressed genes Gapdh and Atp5a1 [29,30].
T7965	29878-29880	TO	denotes	To
T7966	29881-29886	VB	denotes	check
T7968	29887-29890	DT	denotes	the
T7970	29891-29899	JJ	denotes	relative
T7969	29900-29914	NN	denotes	representation
T7971	29915-29917	IN	denotes	of
T7972	29918-29924	NNS	denotes	clones
T7973	29925-29927	IN	denotes	in
T7974	29928-29931	DT	denotes	the
T7976	29932-29936	NN	denotes	cDNA
T7975	29937-29944	NN	denotes	library
T7977	29944-29946	,	denotes	,
T7978	29946-29963	JJ	denotes	semi-quantitative
T7979	29964-29967	NN	denotes	PCR
T7980	29968-29971	VBD	denotes	was
T7967	29972-29981	VBN	denotes	performed
T7981	29982-29984	IN	denotes	on
T7982	29985-29989	NN	denotes	Pax6
T7983	29989-29991	,	denotes	,
T7984	29991-29997	NN	denotes	Homer3
T7985	29997-29999	,	denotes	,
T7986	29999-30004	NN	denotes	Dncl1
T7987	30004-30006	,	denotes	,
T7988	30006-30012	NN	denotes	Trim11
T7989	30013-30016	CC	denotes	and
T7990	30017-30020	DT	denotes	the
T7992	30021-30035	RB	denotes	constitutively
T7993	30036-30045	VBN	denotes	expressed
T7991	30046-30051	NNS	denotes	genes
T7994	30052-30057	NN	denotes	Gapdh
T7995	30058-30061	CC	denotes	and
T7996	30062-30068	NN	denotes	Atp5a1
T7997	30069-30070	-LRB-	denotes	[
T7999	30070-30072	CD	denotes	29
T8000	30072-30073	,	denotes	,
T7998	30073-30075	CD	denotes	30
T8001	30075-30076	-RRB-	denotes	]
T8002	30076-30077	.	denotes	.
T8003	30077-30183	sentence	denotes	Primers were designed to cross at least one intron, so that only correctly spliced clones were amplified.
T8004	30078-30085	NNS	denotes	Primers
T8006	30086-30090	VBD	denotes	were
T8005	30091-30099	VBN	denotes	designed
T8007	30100-30102	TO	denotes	to
T8008	30103-30108	VB	denotes	cross
T8009	30109-30111	RB	denotes	at
T8011	30112-30117	RBS	denotes	least
T8010	30118-30121	CD	denotes	one
T8012	30122-30128	NN	denotes	intron
T8013	30128-30130	,	denotes	,
T8014	30130-30132	IN	denotes	so
T8016	30133-30137	IN	denotes	that
T8017	30138-30142	RB	denotes	only
T8019	30143-30152	RB	denotes	correctly
T8020	30153-30160	VBN	denotes	spliced
T8018	30161-30167	NNS	denotes	clones
T8021	30168-30172	VBD	denotes	were
T8015	30173-30182	VBN	denotes	amplified
T8022	30182-30183	.	denotes	.
T8023	30183-30643	sentence	denotes	Primer sequences were: Pax6-F CAG CCA AAA TAG ATC TAC CTG; Pax6-R CGA TCA CAT GCT CTC TCC TT; Homer3-F CCC AGG TGG CTG TAG AGC; Homer3-R CTC TAC ACA GTG CAA AGC TCA G; Trim11-F GTG CAG GAT GTG AAG CTG; Trim11-R GCC TGC AGA TAG TCA TAG GG; Dncl1-F CAA AAA TGC AGA CAT GTC G; Dncl1-R CTA AGG GAG AAA AAA ATG GGG; Gapdh-F: CAT CAC CAT CTT CCA GGA GC; Gapdh-R: ATG ACC TTG CCC ACA GCC TT; Atp5a1-F: CAC ACG TGA GAT GTC CTC CA; Atp5a1-R: CAC AGA GAT TCG GGG ATA A.
T8024	30184-30190	NN	denotes	Primer
T8025	30191-30200	NNS	denotes	sequences
T8026	30201-30205	VBD	denotes	were
T8027	30205-30207	:	denotes	:
T8028	30207-30211	NN	denotes	Pax6
T8029	30211-30212	HYPH	denotes	-
T8030	30212-30213	NN	denotes	F
T8032	30214-30217	NN	denotes	CAG
T8033	30218-30221	NN	denotes	CCA
T8034	30222-30225	NN	denotes	AAA
T8035	30226-30229	NN	denotes	TAG
T8036	30230-30233	NN	denotes	ATC
T8037	30234-30237	NN	denotes	TAC
T8031	30238-30241	NN	denotes	CTG
T8038	30241-30242	:	denotes	;
T8039	30243-30247	NN	denotes	Pax6
T8040	30247-30248	HYPH	denotes	-
T8041	30248-30249	NN	denotes	R
T8043	30250-30253	NN	denotes	CGA
T8044	30254-30257	NN	denotes	TCA
T8045	30258-30261	NN	denotes	CAT
T8046	30262-30265	NN	denotes	GCT
T8047	30266-30269	NN	denotes	CTC
T8048	30270-30273	NN	denotes	TCC
T8042	30274-30276	NN	denotes	TT
T8049	30276-30277	:	denotes	;
T8050	30278-30284	NN	denotes	Homer3
T8051	30284-30285	HYPH	denotes	-
T8052	30285-30286	NN	denotes	F
T8054	30287-30290	NN	denotes	CCC
T8055	30291-30294	NN	denotes	AGG
T8056	30295-30298	NN	denotes	TGG
T8057	30299-30302	NN	denotes	CTG
T8058	30303-30306	NN	denotes	TAG
T8053	30307-30310	NN	denotes	AGC
T8059	30310-30311	:	denotes	;
T8060	30312-30318	NN	denotes	Homer3
T8061	30318-30319	HYPH	denotes	-
T8062	30319-30320	NN	denotes	R
T8064	30321-30324	NN	denotes	CTC
T8065	30325-30328	NN	denotes	TAC
T8066	30329-30332	NN	denotes	ACA
T8067	30333-30336	NN	denotes	GTG
T8068	30337-30340	NN	denotes	CAA
T8069	30341-30344	NN	denotes	AGC
T8070	30345-30348	NN	denotes	TCA
T8063	30349-30350	NN	denotes	G
T8071	30350-30351	:	denotes	;
T8072	30352-30358	NN	denotes	Trim11
T8073	30358-30359	HYPH	denotes	-
T8074	30359-30360	NN	denotes	F
T8076	30361-30364	NN	denotes	GTG
T8077	30365-30368	NN	denotes	CAG
T8078	30369-30372	NN	denotes	GAT
T8079	30373-30376	NN	denotes	GTG
T8080	30377-30380	NN	denotes	AAG
T8075	30381-30384	NN	denotes	CTG
T8081	30384-30385	:	denotes	;
T8082	30386-30392	NN	denotes	Trim11
T8083	30392-30393	HYPH	denotes	-
T8084	30393-30394	NN	denotes	R
T8086	30395-30398	NN	denotes	GCC
T8087	30399-30402	NN	denotes	TGC
T8088	30403-30406	NN	denotes	AGA
T8089	30407-30410	NN	denotes	TAG
T8090	30411-30414	NN	denotes	TCA
T8091	30415-30418	NN	denotes	TAG
T8085	30419-30421	NN	denotes	GG
T8092	30421-30422	:	denotes	;
T8093	30423-30428	NN	denotes	Dncl1
T8094	30428-30429	HYPH	denotes	-
T8095	30429-30430	NN	denotes	F
T8097	30431-30434	NN	denotes	CAA
T8098	30435-30438	NN	denotes	AAA
T8099	30439-30442	NN	denotes	TGC
T8100	30443-30446	NNP	denotes	AGA
T8101	30447-30450	NNP	denotes	CAT
T8102	30451-30454	NNP	denotes	GTC
T8096	30455-30456	NN	denotes	G
T8103	30456-30457	:	denotes	;
T8104	30458-30463	NN	denotes	Dncl1
T8105	30463-30464	HYPH	denotes	-
T8106	30464-30465	NN	denotes	R
T8108	30466-30469	NN	denotes	CTA
T8109	30470-30473	NN	denotes	AGG
T8110	30474-30477	NN	denotes	GAG
T8111	30478-30481	NN	denotes	AAA
T8112	30482-30485	NN	denotes	AAA
T8113	30486-30489	NN	denotes	ATG
T8107	30490-30493	NN	denotes	GGG
T8114	30493-30494	:	denotes	;
T8115	30495-30500	NN	denotes	Gapdh
T8117	30500-30501	HYPH	denotes	-
T8116	30501-30502	NN	denotes	F
T8118	30502-30504	:	denotes	:
T8119	30504-30507	NN	denotes	CAT
T8121	30508-30511	NN	denotes	CAC
T8122	30512-30515	NN	denotes	CAT
T8123	30516-30519	NN	denotes	CTT
T8124	30520-30523	NN	denotes	CCA
T8125	30524-30527	NN	denotes	GGA
T8120	30528-30530	NN	denotes	GC
T8126	30530-30531	:	denotes	;
T8127	30532-30537	NN	denotes	Gapdh
T8129	30537-30538	HYPH	denotes	-
T8128	30538-30539	NN	denotes	R
T8130	30539-30541	:	denotes	:
T8131	30541-30544	NN	denotes	ATG
T8133	30545-30548	NN	denotes	ACC
T8134	30549-30552	NN	denotes	TTG
T8135	30553-30556	NN	denotes	CCC
T8136	30557-30560	NN	denotes	ACA
T8137	30561-30564	NN	denotes	GCC
T8132	30565-30567	NN	denotes	TT
T8138	30567-30568	:	denotes	;
T8139	30569-30575	NN	denotes	Atp5a1
T8141	30575-30576	HYPH	denotes	-
T8140	30576-30577	NN	denotes	F
T8142	30577-30579	:	denotes	:
T8143	30579-30582	NN	denotes	CAC
T8145	30583-30586	NN	denotes	ACG
T8146	30587-30590	NN	denotes	TGA
T8147	30591-30594	NN	denotes	GAT
T8148	30595-30598	NN	denotes	GTC
T8149	30599-30602	NN	denotes	CTC
T8144	30603-30605	NN	denotes	CA
T8150	30605-30606	:	denotes	;
T8151	30607-30613	NN	denotes	Atp5a1
T8153	30613-30614	HYPH	denotes	-
T8152	30614-30615	NN	denotes	R
T8154	30615-30617	:	denotes	:
T8155	30617-30620	NN	denotes	CAC
T8157	30621-30624	NN	denotes	AGA
T8158	30625-30628	NN	denotes	GAT
T8159	30629-30632	NN	denotes	TCG
T8160	30633-30636	NN	denotes	GGG
T8161	30637-30640	NN	denotes	ATA
T8156	30641-30642	NN	denotes	A
T8162	30642-30643	.	denotes	.
T8163	30643-30832	sentence	denotes	10 ng library cDNA were amplified in a reaction containing 1xAmpliTaq polymerase buffer (Perkin Elmer), 1.5 mM MgCl2, 200μM each primer and 2.5 units of AmpliTaq polymerase (Perkin Elmer).
T8164	30644-30646	CD	denotes	10
T8165	30647-30649	NN	denotes	ng
T8167	30650-30657	NN	denotes	library
T8166	30658-30662	NN	denotes	cDNA
T8169	30663-30667	VBD	denotes	were
T8168	30668-30677	VBN	denotes	amplified
T8170	30678-30680	IN	denotes	in
T8171	30681-30682	DT	denotes	a
T8172	30683-30691	NN	denotes	reaction
T8173	30692-30702	VBG	denotes	containing
T8174	30703-30704	CD	denotes	1
T8176	30704-30705	SYM	denotes	x
T8177	30705-30713	NNP	denotes	AmpliTaq
T8178	30714-30724	NN	denotes	polymerase
T8175	30725-30731	NN	denotes	buffer
T8179	30732-30733	-LRB-	denotes	(
T8181	30733-30739	NNP	denotes	Perkin
T8180	30740-30745	NNP	denotes	Elmer
T8182	30745-30746	-RRB-	denotes	)
T8183	30746-30748	,	denotes	,
T8184	30748-30751	CD	denotes	1.5
T8185	30752-30754	NN	denotes	mM
T8186	30755-30760	NN	denotes	MgCl2
T8187	30760-30762	,	denotes	,
T8188	30762-30767	NN	denotes	200μM
T8190	30768-30772	DT	denotes	each
T8189	30773-30779	NN	denotes	primer
T8191	30780-30783	CC	denotes	and
T8192	30784-30787	CD	denotes	2.5
T8193	30788-30793	NNS	denotes	units
T8194	30794-30796	IN	denotes	of
T8195	30797-30805	NNP	denotes	AmpliTaq
T8196	30806-30816	NN	denotes	polymerase
T8197	30817-30818	-LRB-	denotes	(
T8199	30818-30824	NNP	denotes	Perkin
T8198	30825-30830	NNP	denotes	Elmer
T8200	30830-30831	-RRB-	denotes	)
T8201	30831-30832	.	denotes	.
T8202	30832-30958	sentence	denotes	PCR conditions were (95°C for 30 sec) × 1, (94°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec) × 32 and (72°C for 2 min) × 1.
T8203	30833-30836	NN	denotes	PCR
T8204	30837-30847	NNS	denotes	conditions
T8205	30848-30852	VBD	denotes	were
T8206	30853-30854	-LRB-	denotes	(
T8207	30854-30856	CD	denotes	95
T8208	30856-30858	NN	denotes	°C
T8209	30859-30862	IN	denotes	for
T8210	30863-30865	CD	denotes	30
T8211	30866-30869	NN	denotes	sec
T8212	30869-30870	-RRB-	denotes	)
T8213	30871-30872	SYM	denotes	×
T8214	30873-30874	CD	denotes	1
T8215	30874-30876	,	denotes	,
T8216	30876-30877	-LRB-	denotes	(
T8218	30877-30879	CD	denotes	94
T8217	30879-30881	NN	denotes	°C
T8219	30882-30885	IN	denotes	for
T8220	30886-30888	CD	denotes	30
T8221	30889-30892	NN	denotes	sec
T8222	30892-30894	,	denotes	,
T8223	30894-30896	CD	denotes	55
T8224	30896-30898	NN	denotes	°C
T8225	30899-30902	IN	denotes	for
T8226	30903-30905	CD	denotes	30
T8227	30906-30909	NN	denotes	sec
T8228	30909-30911	,	denotes	,
T8229	30911-30913	CD	denotes	72
T8230	30913-30915	NN	denotes	°C
T8231	30916-30919	IN	denotes	for
T8232	30920-30922	CD	denotes	30
T8233	30923-30926	NN	denotes	sec
T8234	30926-30927	-RRB-	denotes	)
T8235	30928-30929	SYM	denotes	×
T8236	30930-30932	CD	denotes	32
T8237	30933-30936	CC	denotes	and
T8238	30937-30938	-LRB-	denotes	(
T8240	30938-30940	CD	denotes	72
T8239	30940-30942	NN	denotes	°C
T8241	30943-30946	IN	denotes	for
T8242	30947-30948	CD	denotes	2
T8243	30949-30952	NN	denotes	min
T8244	30952-30953	-RRB-	denotes	)
T8245	30954-30955	SYM	denotes	×
T8246	30956-30957	CD	denotes	1
T8247	30957-30958	.	denotes	.
T8248	30958-31045	sentence	denotes	Products were resolved on a 2.5% agarose gel with ΦX174/HaeIII size markers (Promega).
T8249	30959-30967	NNS	denotes	Products
T8251	30968-30972	VBD	denotes	were
T8250	30973-30981	VBN	denotes	resolved
T8252	30982-30984	IN	denotes	on
T8253	30985-30986	DT	denotes	a
T8255	30987-30990	CD	denotes	2.5
T8256	30990-30991	NN	denotes	%
T8257	30992-30999	NN	denotes	agarose
T8254	31000-31003	NN	denotes	gel
T8258	31004-31008	IN	denotes	with
T8259	31009-31014	NN	denotes	ΦX174
T8261	31014-31015	HYPH	denotes	/
T8260	31015-31021	NN	denotes	HaeIII
T8263	31022-31026	NN	denotes	size
T8262	31027-31034	NNS	denotes	markers
T8264	31035-31036	-LRB-	denotes	(
T8265	31036-31043	NNP	denotes	Promega
T8266	31043-31044	-RRB-	denotes	)
T8267	31044-31045	.	denotes	.
R10	T186	T187	npadvmod	C,terminal
R100	T289	T271	punct	.,used
R1000	T1591	T1592	npadvmod	C,terminal
R1001	T1592	T1590	amod	terminal,end
R1002	T1593	T1592	punct	-,terminal
R1003	T1594	T1590	prep	of,end
R1004	T1595	T1596	det	the,protein
R1005	T1596	T1594	pobj	protein,of
R1006	T1597	T1596	compound	PAX6,protein
R1007	T1598	T1570	punct	.,used
R1008	T1600	T1601	nsubj	We,screened
R1009	T1602	T1601	advmod	then,screened
R101	T291	T292	nmod	Yeast,screens
R1010	T1603	T1604	det	a,library
R1011	T1604	T1601	dobj	library,screened
R1012	T1605	T1604	compound	brain,library
R1013	T1606	T1601	prep	with,screened
R1014	T1607	T1608	det	this,peptide
R1015	T1608	T1606	pobj	peptide,with
R1016	T1609	T1601	advcl	using,screened
R1017	T1610	T1611	det	the,technique
R1018	T1611	T1609	dobj	technique,using
R1019	T1612	T1611	nmod	yeast,technique
R102	T292	T297	nsubjpass	screens,carried
R1020	T1765	T1766	det	A,peptide
R1021	T1767	T1768	advmod	highly,conserved
R1022	T1768	T1766	amod	conserved,peptide
R1023	T1769	T1770	npadvmod	C,terminal
R1024	T1770	T1766	amod	terminal,peptide
R1025	T1771	T1770	punct	-,terminal
R1026	T1772	T1766	compound	PAX6,peptide
R1027	T1774	T1775	nsubj	We,noted
R1028	T1776	T1774	cc	and,We
R1029	T1777	T1774	conj	others,We
R103	T293	T294	nummod	two,hybrid
R1030	T1778	T1779	punct	[,31
R1031	T1779	T1777	parataxis	31,others
R1032	T1780	T1781	punct	-,33
R1033	T1781	T1779	prep	33,31
R1034	T1782	T1779	punct	],31
R1035	T1783	T1784	mark	that,is
R1036	T1784	T1775	ccomp	is,noted
R1037	T1785	T1784	expl	there,is
R1038	T1786	T1787	amod	significant,conservation
R1039	T1787	T1784	attr	conservation,is
R104	T294	T292	compound	hybrid,screens
R1040	T1788	T1787	compound	sequence,conservation
R1041	T1789	T1784	prep	at,is
R1042	T1790	T1791	det	the,end
R1043	T1791	T1789	pobj	end,at
R1044	T1792	T1793	npadvmod	C,terminal
R1045	T1793	T1791	amod	terminal,end
R1046	T1794	T1793	punct	-,terminal
R1047	T1795	T1791	prep	of,end
R1048	T1796	T1797	det	the,protein
R1049	T1797	T1795	pobj	protein,of
R105	T295	T294	punct	-,hybrid
R1050	T1798	T1797	compound	PAX6,protein
R1051	T1799	T1775	punct	.,noted
R1052	T1801	T1802	compound	BLAST,analysis
R1053	T1802	T1803	nsubj	analysis,revealed
R1054	T1804	T1802	prep	of,analysis
R1055	T1805	T1806	det	the,sequence
R1056	T1806	T1804	pobj	sequence,of
R1057	T1807	T1808	compound	amino,acid
R1058	T1808	T1806	compound	acid,sequence
R1059	T1809	T1806	prep	of,sequence
R106	T296	T292	compound	library,screens
R1060	T1810	T1811	det	the,domain
R1061	T1811	T1809	pobj	domain,of
R1062	T1812	T1811	compound	PAX6,domain
R1063	T1813	T1811	compound	PST,domain
R1064	T1814	T1815	punct	(,278
R1065	T1815	T1806	parataxis	278,sequence
R1066	T1816	T1815	nmod	aa,278
R1067	T1817	T1818	punct	–,422
R1068	T1818	T1815	prep	422,278
R1069	T1819	T1815	punct	),278
R107	T298	T297	auxpass	were,carried
R1070	T1820	T1821	det	a,motif
R1071	T1821	T1803	dobj	motif,revealed
R1072	T1822	T1823	advmod	highly,conserved
R1073	T1823	T1821	amod	conserved,motif
R1074	T1824	T1821	prep	within,motif
R1075	T1825	T1826	det	the,acids
R1076	T1826	T1824	pobj	acids,within
R1077	T1827	T1826	amod	last,acids
R1078	T1828	T1826	nummod	28,acids
R1079	T1829	T1826	compound	amino,acids
R108	T299	T297	advmod	then,carried
R1080	T1830	T1821	punct	(,motif
R1081	T1831	T1821	acl	beginning,motif
R1082	T1832	T1831	prep	at,beginning
R1083	T1833	T1834	det	the,motif
R1084	T1834	T1832	pobj	motif,at
R1085	T1835	T1834	punct	',motif
R1086	T1836	T1834	nmod	GLISP,motif
R1087	T1837	T1834	punct	',motif
R1088	T1838	T1839	punct	", ",Figure
R1089	T1839	T1803	parataxis	Figure,revealed
R109	T300	T297	prt	out,carried
R1090	T1840	T1839	nummod	1a,Figure
R1091	T1841	T1839	punct	),Figure
R1092	T1842	T1803	punct	.,revealed
R1093	T1844	T1845	amod	Strong,conservation
R1094	T1845	T1846	nsubjpass	conservation,seen
R1095	T1847	T1846	auxpass	was,seen
R1096	T1848	T1846	prep	in,seen
R1097	T1849	T1850	advmod	distantly,related
R1098	T1850	T1851	amod	related,species
R1099	T1851	T1848	pobj	species,in
R11	T187	T189	amod	terminal,mutations
R110	T301	T302	aux	to,identify
R1100	T1852	T1853	amod	such,as
R1101	T1853	T1851	prep	as,species
R1102	T1854	T1853	pobj	axolotl,as
R1103	T1855	T1854	punct	(,axolotl
R1104	T1856	T1857	compound	Ambystoma,mexicanum
R1105	T1857	T1854	appos	mexicanum,axolotl
R1106	T1858	T1854	punct	),axolotl
R1107	T1859	T1854	cc	and,axolotl
R1108	T1860	T1861	compound	sea,urchin
R1109	T1861	T1854	conj	urchin,axolotl
R111	T302	T297	advcl	identify,carried
R1110	T1862	T1861	punct	(,urchin
R1111	T1863	T1864	compound	Paracentrotus,lividus
R1112	T1864	T1861	appos	lividus,urchin
R1113	T1865	T1846	punct	),seen
R1114	T1866	T1867	punct	(,Figure
R1115	T1867	T1846	parataxis	Figure,seen
R1116	T1868	T1867	nummod	1a,Figure
R1117	T1869	T1867	punct	),Figure
R1118	T1870	T1846	punct	.,seen
R1119	T1872	T1873	nsubj	We,subjected
R112	T303	T304	npadvmod	brain,expressed
R1120	T1874	T1875	det	the,domain
R1121	T1875	T1873	dobj	domain,subjected
R1122	T1876	T1875	amod	whole,domain
R1123	T1877	T1875	compound	PAX6,domain
R1124	T1878	T1875	compound	PST,domain
R1125	T1879	T1873	prep	to,subjected
R1126	T1880	T1881	amod	secondary,analysis
R1127	T1881	T1879	pobj	analysis,to
R1128	T1882	T1881	compound	structure,analysis
R1129	T1883	T1873	advcl	using,subjected
R113	T304	T306	amod	expressed,proteins
R1130	T1884	T1883	dobj	JPRED,using
R1131	T1885	T1884	punct	", ",JPRED
R1132	T1886	T1887	det	a,program
R1133	T1887	T1884	appos	program,JPRED
R1134	T1888	T1889	dep	that,uses
R1135	T1889	T1887	relcl	uses,program
R1136	T1890	T1891	det	a,number
R1137	T1891	T1889	dobj	number,uses
R1138	T1892	T1891	prep	of,number
R1139	T1893	T1894	amod	different,algorithms
R114	T305	T304	punct	-,expressed
R1140	T1894	T1892	pobj	algorithms,of
R1141	T1895	T1896	compound	protein,structure
R1142	T1896	T1897	compound	structure,prediction
R1143	T1897	T1894	compound	prediction,algorithms
R1144	T1898	T1899	aux	to,generate
R1145	T1899	T1889	advcl	generate,uses
R1146	T1900	T1901	det	a,structure
R1147	T1901	T1899	dobj	structure,generate
R1148	T1902	T1901	nmod	consensus,structure
R1149	T1903	T1901	amod	secondary,structure
R115	T306	T302	dobj	proteins,identify
R1150	T1904	T1905	punct	(,Figure
R1151	T1905	T1873	parataxis	Figure,subjected
R1152	T1906	T1905	nummod	1,Figure
R1153	T1907	T1905	punct	),Figure
R1154	T1908	T1909	punct	[,26
R1155	T1909	T1873	parataxis	26,subjected
R1156	T1910	T1909	nummod	25,26
R1157	T1911	T1909	punct	",",26
R1158	T1912	T1909	punct	],26
R1159	T1913	T1873	punct	.,subjected
R116	T307	T308	dep	that,interact
R1160	T1915	T1916	det	The,domain
R1161	T1916	T1918	nsubj	domain,was
R1162	T1917	T1916	compound	PST,domain
R1163	T1919	T1918	advmod	largely,was
R1164	T1920	T1918	acomp	devoid,was
R1165	T1921	T1920	prep	of,devoid
R1166	T1922	T1923	amod	predicted,structure
R1167	T1923	T1921	pobj	structure,of
R1168	T1924	T1923	amod	secondary,structure
R1169	T1925	T1918	prep	except,was
R117	T308	T306	relcl	interact,proteins
R1170	T1926	T1925	prep	for,except
R1171	T1927	T1928	det	the,region
R1172	T1928	T1926	pobj	region,for
R1173	T1929	T1930	npadvmod	C,terminal
R1174	T1930	T1928	amod	terminal,region
R1175	T1931	T1930	punct	-,terminal
R1176	T1932	T1928	punct	", ",region
R1177	T1933	T1934	dep	which,contained
R1178	T1934	T1928	relcl	contained,region
R1179	T1935	T1936	nummod	two,sheets
R118	T309	T308	prep	with,interact
R1180	T1936	T1934	dobj	sheets,contained
R1181	T1937	T1936	amod	predicted,sheets
R1182	T1938	T1936	compound	beta,sheets
R1183	T1939	T1936	prep	within,sheets
R1184	T1940	T1941	det	the,domain
R1185	T1941	T1939	pobj	domain,within
R1186	T1942	T1941	advmod	highly,domain
R1187	T1943	T1941	amod	conserved,domain
R1188	T1944	T1936	punct	", ",sheets
R1189	T1945	T1936	appos	one,sheets
R119	T310	T311	det	the,peptide
R1190	T1946	T1945	prep	in,one
R1191	T1947	T1948	det	the,GLISP
R1192	T1948	T1950	nmod	GLISP,motif
R1193	T1949	T1948	punct	',GLISP
R1194	T1950	T1946	pobj	motif,in
R1195	T1951	T1950	punct	',motif
R1196	T1952	T1945	cc	and,one
R1197	T1953	T1945	conj	one,one
R1198	T1954	T1953	prep	in,one
R1199	T1955	T1956	det	the,SPVPQ
R12	T188	T187	punct	-,terminal
R120	T311	T309	pobj	peptide,with
R1200	T1956	T1958	nmod	SPVPQ,motif
R1201	T1957	T1956	punct	',SPVPQ
R1202	T1958	T1954	pobj	motif,in
R1203	T1959	T1958	punct	',motif
R1204	T1960	T1961	punct	(,identical
R1205	T1961	T1918	parataxis	identical,was
R1206	T1962	T1961	prep	to,identical
R1207	T1963	T1964	det	the,pattern
R1208	T1964	T1962	pobj	pattern,to
R1209	T1965	T1964	acl	shown,pattern
R121	T312	T313	npadvmod	C,terminal
R1210	T1966	T1965	prep	in,shown
R1211	T1967	T1966	pobj	Figure,in
R1212	T1968	T1967	nummod	1c,Figure
R1213	T1969	T1961	punct	),identical
R1214	T1970	T1918	punct	.,was
R1215	T1972	T1973	advmod	Initially,defined
R1216	T1974	T1973	nsubj	we,defined
R1217	T1975	T1976	det	the,domain
R1218	T1976	T1973	dobj	domain,defined
R1219	T1977	T1978	npadvmod	C,terminal
R122	T313	T311	amod	terminal,peptide
R1220	T1978	T1976	amod	terminal,domain
R1221	T1979	T1978	punct	-,terminal
R1222	T1980	T1973	prep	as,defined
R1223	T1981	T1980	pcomp	running,as
R1224	T1982	T1981	prep	from,running
R1225	T1983	T1984	det	the,GLISP
R1226	T1984	T1986	nmod	GLISP,motif
R1227	T1985	T1984	punct	',GLISP
R1228	T1986	T1982	pobj	motif,from
R1229	T1987	T1984	punct	',GLISP
R123	T314	T313	punct	-,terminal
R1230	T1988	T1981	prep	up,running
R1231	T1989	T1988	prep	to,up
R1232	T1990	T1991	det	the,codon
R1233	T1991	T1989	pobj	codon,to
R1234	T1992	T1991	compound	stop,codon
R1235	T1993	T1973	punct	", ",defined
R1236	T1994	T1995	mark	since,conserved
R1237	T1995	T1973	advcl	conserved,defined
R1238	T1996	T1997	det	this,region
R1239	T1997	T1995	nsubjpass	region,conserved
R124	T315	T309	cc	and,with
R1240	T1998	T1995	auxpass	was,conserved
R1241	T1999	T2000	advmod	most,highly
R1242	T2000	T1995	advmod	highly,conserved
R1243	T2001	T1995	cc	and,conserved
R1244	T2002	T1995	conj	contained,conserved
R1245	T2003	T2004	advmod	strongly,predicted
R1246	T2004	T2005	amod	predicted,elements
R1247	T2005	T2002	dobj	elements,contained
R1248	T2006	T2007	amod	secondary,structure
R1249	T2007	T2005	compound	structure,elements
R125	T316	T309	conj	with,with
R1250	T2008	T1973	punct	.,defined
R1251	T2010	T2011	advmod	However,lost
R1252	T2012	T2013	advmod	when,performed
R1253	T2013	T2011	advcl	performed,lost
R1254	T2014	T2013	nsubj	we,performed
R1255	T2015	T2016	amod	secondary,structure
R1256	T2016	T2017	compound	structure,prediction
R1257	T2017	T2018	compound	prediction,analysis
R1258	T2018	T2013	dobj	analysis,performed
R1259	T2019	T2013	prep	on,performed
R126	T317	T318	det	the,domain
R1260	T2020	T2021	det	this,peptide
R1261	T2021	T2019	pobj	peptide,on
R1262	T2022	T2023	nummod	28,acid
R1263	T2023	T2021	compound	acid,peptide
R1264	T2024	T2023	compound	amino,acid
R1265	T2025	T2011	punct	", ",lost
R1266	T2026	T2027	det	the,sheet
R1267	T2027	T2011	nsubjpass	sheet,lost
R1268	T2028	T2027	amod	first,sheet
R1269	T2029	T2027	compound	beta,sheet
R127	T318	T316	pobj	domain,with
R1270	T2030	T2011	auxpass	was,lost
R1271	T2031	T2032	punct	(,Figure
R1272	T2032	T2011	parataxis	Figure,lost
R1273	T2033	T2032	nummod	1b,Figure
R1274	T2034	T2032	punct	),Figure
R1275	T2035	T2011	punct	.,lost
R1276	T2037	T2038	nsubj	Addition,caused
R1277	T2039	T2037	prep	of,Addition
R1278	T2040	T2041	det	another,acids
R1279	T2041	T2039	pobj	acids,of
R128	T319	T318	amod	entire,domain
R1280	T2042	T2041	nummod	4,acids
R1281	T2043	T2041	compound	amino,acids
R1282	T2044	T2045	punct	(,TTST
R1283	T2045	T2037	parataxis	TTST,Addition
R1284	T2046	T2045	punct	),TTST
R1285	T2047	T2048	advmod	immediately,before
R1286	T2048	T2037	prep	before,Addition
R1287	T2049	T2050	punct	',GLISP
R1288	T2050	T2048	pobj	GLISP,before
R1289	T2051	T2050	punct	',GLISP
R129	T320	T318	nmod	PAX6,domain
R1290	T2052	T2038	dobj	recovery,caused
R1291	T2053	T2052	prep	of,recovery
R1292	T2054	T2055	det	the,sheet
R1293	T2055	T2053	pobj	sheet,of
R1294	T2056	T2055	amod	first,sheet
R1295	T2057	T2055	compound	beta,sheet
R1296	T2058	T2059	punct	(,Figure
R1297	T2059	T2038	parataxis	Figure,caused
R1298	T2060	T2059	nummod	1c,Figure
R1299	T2061	T2059	punct	),Figure
R13	T189	T178	nsubj	mutations,disrupt
R130	T321	T322	compound	proline,threonine
R1300	T2062	T2038	punct	.,caused
R1301	T2064	T2065	mark	Although,are
R1302	T2065	T2069	advcl	are,appear
R1303	T2066	T2067	det	these,residues
R1304	T2067	T2065	nsubj	residues,are
R1305	T2068	T2067	nummod	4,residues
R1306	T2070	T2065	neg	not,are
R1307	T2071	T2072	advmod	highly,conserved
R1308	T2072	T2065	acomp	conserved,are
R1309	T2073	T2074	punct	(,Figure
R131	T322	T326	npadvmod	threonine,rich
R1310	T2074	T2065	parataxis	Figure,are
R1311	T2075	T2074	nummod	1a,Figure
R1312	T2076	T2074	punct	),Figure
R1313	T2077	T2069	punct	", ",appear
R1314	T2078	T2069	nsubj	they,appear
R1315	T2079	T2080	aux	to,be
R1316	T2080	T2069	xcomp	be,appear
R1317	T2081	T2080	acomp	important,be
R1318	T2082	T2081	prep	for,important
R1319	T2083	T2082	pcomp	seeding,for
R132	T323	T322	punct	-,threonine
R1320	T2084	T2085	det	the,sheet
R1321	T2085	T2083	dobj	sheet,seeding
R1322	T2086	T2085	amod	first,sheet
R1323	T2087	T2085	compound	beta,sheet
R1324	T2088	T2082	cc	and,for
R1325	T2089	T2090	advmod	therefore,for
R1326	T2090	T2082	conj	for,for
R1327	T2091	T2092	amod	secondary,structure
R1328	T2092	T2090	pobj	structure,for
R1329	T2093	T2069	punct	.,appear
R133	T324	T322	compound	serine,threonine
R1330	T2095	T2096	advmod	Thus,define
R1331	T2097	T2096	nsubj	we,define
R1332	T2098	T2099	det	the,peptide
R1333	T2099	T2096	dobj	peptide,define
R1334	T2100	T2101	npadvmod	C,terminal
R1335	T2101	T2099	amod	terminal,peptide
R1336	T2102	T2101	punct	-,terminal
R1337	T2103	T2096	prep	as,define
R1338	T2104	T2103	pcomp	being,as
R1339	T2105	T2106	det	the,acids
R134	T325	T322	punct	-,threonine
R1340	T2106	T2104	attr	acids,being
R1341	T2107	T2106	amod	final,acids
R1342	T2108	T2106	nummod	32,acids
R1343	T2109	T2106	compound	amino,acids
R1344	T2110	T2106	prep	of,acids
R1345	T2111	T2110	pobj	PAX6,of
R1346	T2112	T2106	punct	", ",acids
R1347	T2113	T2106	acl	running,acids
R1348	T2114	T2113	prep	from,running
R1349	T2115	T2114	pobj	threonine,from
R135	T326	T318	amod	rich,domain
R1350	T2116	T2115	nummod	391,threonine
R1351	T2117	T2113	prep	to,running
R1352	T2118	T2119	det	the,codon
R1353	T2119	T2117	pobj	codon,to
R1354	T2120	T2119	compound	stop,codon
R1355	T2121	T2122	punct	(,line
R1356	T2122	T2096	parataxis	line,define
R1357	T2123	T2122	compound	top,line
R1358	T2124	T2122	prep	of,line
R1359	T2125	T2124	pobj	Figure,of
R136	T327	T326	punct	-,rich
R1360	T2126	T2125	nummod	1c,Figure
R1361	T2127	T2122	punct	),line
R1362	T2128	T2096	punct	.,define
R1365	T2344	T2345	nmod	Yeast,screening
R1366	T2346	T2347	nummod	two,hybrid
R1367	T2347	T2345	compound	hybrid,screening
R1368	T2348	T2347	punct	-,hybrid
R1369	T2350	T2351	nsubj	We,hypothesised
R137	T328	T297	punct	.,carried
R1370	T2352	T2353	mark	that,involved
R1371	T2353	T2351	ccomp	involved,hypothesised
R1372	T2354	T2355	det	the,peptide
R1373	T2355	T2353	nsubjpass	peptide,involved
R1374	T2356	T2357	npadvmod	C,terminal
R1375	T2357	T2355	amod	terminal,peptide
R1376	T2358	T2357	punct	-,terminal
R1377	T2359	T2353	aux	might,involved
R1378	T2360	T2353	auxpass	be,involved
R1379	T2361	T2353	prep	in,involved
R138	T330	T331	nummod	Three,proteins
R1380	T2362	T2363	nmod	protein,protein
R1381	T2363	T2365	compound	protein,interactions
R1382	T2364	T2363	punct	-,protein
R1383	T2365	T2361	pobj	interactions,in
R1384	T2366	T2351	punct	", ",hypothesised
R1385	T2367	T2351	cc	and,hypothesised
R1386	T2368	T2369	nsubj	we,tested
R1387	T2369	T2351	conj	tested,hypothesised
R1388	T2370	T2369	dobj	this,tested
R1389	T2371	T2369	prep	by,tested
R139	T331	T336	nsubjpass	proteins,identified
R1390	T2372	T2371	pcomp	screening,by
R1391	T2373	T2374	det	a,library
R1392	T2374	T2372	dobj	library,screening
R1393	T2375	T2374	compound	cDNA,library
R1394	T2376	T2372	advcl	using,screening
R1395	T2377	T2378	det	the,system
R1396	T2378	T2376	dobj	system,using
R1397	T2379	T2378	nmod	yeast,system
R1398	T2380	T2381	nummod	two,hybrid
R1399	T2381	T2378	compound	hybrid,system
R14	T190	T178	dobj	interaction,disrupt
R140	T332	T331	amod	novel,proteins
R1400	T2382	T2381	punct	-,hybrid
R1401	T2383	T2378	prep	with,system
R1402	T2384	T2385	det	a,construct
R1403	T2385	T2383	pobj	construct,with
R1404	T2386	T2387	punct	(,PAX6CTP
R1405	T2387	T2385	parataxis	PAX6CTP,construct
R1406	T2388	T2387	punct	),PAX6CTP
R1407	T2389	T2390	prep	in,fused
R1408	T2390	T2385	relcl	fused,construct
R1409	T2391	T2389	pobj	which,in
R141	T333	T334	npadvmod	PAX6,interacting
R1410	T2392	T2393	det	the,peptide
R1411	T2393	T2390	nsubjpass	peptide,fused
R1412	T2394	T2395	nummod	32,acid
R1413	T2395	T2393	nmod	acid,peptide
R1414	T2396	T2395	nmod	amino,acid
R1415	T2397	T2398	npadvmod	C,terminal
R1416	T2398	T2393	amod	terminal,peptide
R1417	T2399	T2398	punct	-,terminal
R1418	T2400	T2390	auxpass	was,fused
R1419	T2401	T2390	prep	to,fused
R142	T334	T331	amod	interacting,proteins
R1420	T2402	T2403	det	the,domain
R1421	T2403	T2401	pobj	domain,to
R1422	T2404	T2403	compound	yeast,domain
R1423	T2405	T2403	compound	GAL4,domain
R1424	T2406	T2407	compound	DNA,binding
R1425	T2407	T2403	compound	binding,domain
R1426	T2408	T2369	punct	.,tested
R1427	T2410	T2411	nsubj	We,chose
R1428	T2412	T2413	aux	to,screen
R1429	T2413	T2411	xcomp	screen,chose
R143	T335	T334	punct	-,interacting
R1430	T2414	T2415	det	a,library
R1431	T2415	T2413	dobj	library,screen
R1432	T2416	T2417	compound	mouse,brain
R1433	T2417	T2415	compound	brain,library
R1434	T2418	T2415	compound	cDNA,library
R1435	T2419	T2420	mark	as,were
R1436	T2420	T2411	advcl	were,chose
R1437	T2421	T2422	det	no,libraries
R1438	T2422	T2420	nsubj	libraries,were
R1439	T2423	T2422	amod	human,libraries
R144	T337	T336	auxpass	were,identified
R1440	T2424	T2420	acomp	available,were
R1441	T2425	T2411	punct	.,chose
R1442	T2427	T2428	prep	Given,reasoned
R1443	T2429	T2430	det	the,fact
R1444	T2430	T2427	pobj	fact,Given
R1445	T2431	T2432	mark	that,is
R1446	T2432	T2430	acl	is,fact
R1447	T2433	T2434	det	the,sequence
R1448	T2434	T2432	nsubj	sequence,is
R1449	T2435	T2436	compound	amino,acid
R145	T338	T336	punct	: ,identified
R1450	T2436	T2434	compound	acid,sequence
R1451	T2437	T2434	prep	of,sequence
R1452	T2438	T2439	det	the,protein
R1453	T2439	T2437	pobj	protein,of
R1454	T2440	T2439	compound	PAX6,protein
R1455	T2441	T2432	acomp	identical,is
R1456	T2442	T2432	prep	in,is
R1457	T2443	T2442	pobj	man,in
R1458	T2444	T2443	cc	and,man
R1459	T2445	T2443	conj	mouse,man
R146	T339	T340	det	the,protein
R1460	T2446	T2428	punct	", ",reasoned
R1461	T2447	T2428	nsubj	we,reasoned
R1462	T2448	T2449	mark	that,yield
R1463	T2449	T2428	ccomp	yield,reasoned
R1464	T2450	T2451	det	a,library
R1465	T2451	T2449	nsubj	library,yield
R1466	T2452	T2453	compound	mouse,brain
R1467	T2453	T2451	compound	brain,library
R1468	T2454	T2449	aux	would,yield
R1469	T2455	T2456	amod	relevant,interactors
R147	T340	T336	dep	protein,identified
R1470	T2456	T2449	dobj	interactors,yield
R1471	T2457	T2428	punct	.,reasoned
R1472	T2459	T2460	prep	For,carried
R1473	T2461	T2459	pobj	comparison,For
R1474	T2462	T2460	nsubj	we,carried
R1475	T2463	T2460	advmod	also,carried
R1476	T2464	T2460	prt	out,carried
R1477	T2465	T2466	det	the,screen
R1478	T2466	T2460	dobj	screen,carried
R1479	T2467	T2460	advcl	using,carried
R148	T341	T342	amod	post-synaptic,density
R1480	T2468	T2469	det	a,construct
R1481	T2469	T2467	dobj	construct,using
R1482	T2470	T2469	acl	containing,construct
R1483	T2471	T2472	det	the,domain
R1484	T2472	T2470	dobj	domain,containing
R1485	T2473	T2472	amod	whole,domain
R1486	T2474	T2472	compound	PST,domain
R1487	T2475	T2469	punct	(,construct
R1488	T2476	T2469	appos	PAX6PST,construct
R1489	T2477	T2469	punct	),construct
R149	T342	T340	nmod	density,protein
R1490	T2478	T2460	punct	.,carried
R1491	T2480	T2481	det	The,screen
R1492	T2481	T2486	nsubj	screen,gave
R1493	T2482	T2483	npadvmod	C,terminal
R1494	T2483	T2481	amod	terminal,screen
R1495	T2484	T2483	punct	-,terminal
R1496	T2485	T2481	compound	peptide,screen
R1497	T2487	T2488	nummod	15,colonies
R1498	T2488	T2486	dobj	colonies,gave
R1499	T2489	T2490	dep	that,were
R15	T191	T190	prep	with,interaction
R150	T343	T342	punct	(,density
R1500	T2490	T2488	relcl	were,colonies
R1501	T2491	T2490	acomp	positive,were
R1502	T2492	T2491	prep	with,positive
R1503	T2493	T2494	det	all,reporters
R1504	T2494	T2492	pobj	reporters,with
R1505	T2495	T2494	nummod	three,reporters
R1506	T2496	T2486	cc	and,gave
R1507	T2497	T2498	det	the,screen
R1508	T2498	T2501	nsubj	screen,gave
R1509	T2499	T2498	compound	PST,screen
R151	T344	T342	appos	PSD,density
R1510	T2500	T2498	compound	domain,screen
R1511	T2501	T2486	conj	gave,gave
R1512	T2502	T2503	nummod	62,colonies
R1513	T2503	T2501	dobj	colonies,gave
R1514	T2504	T2501	punct	.,gave
R1515	T2506	T2507	det	The,plasmids
R1516	T2507	T2509	nsubjpass	plasmids,isolated
R1517	T2508	T2507	amod	interacting,plasmids
R1518	T2510	T2509	auxpass	were,isolated
R1519	T2511	T2509	cc	and,isolated
R152	T345	T340	punct	),protein
R1520	T2512	T2513	det	the,inserts
R1521	T2513	T2515	nsubj	inserts,sequenced
R1522	T2514	T2513	compound	cDNA,inserts
R1523	T2515	T2509	conj	sequenced,isolated
R1524	T2516	T2515	punct	.,sequenced
R1525	T2518	T2519	nummod	Three,cDNAs
R1526	T2519	T2520	nsubjpass	cDNAs,identified
R1527	T2521	T2520	auxpass	were,identified
R1528	T2522	T2523	nummod	3,times
R1529	T2523	T2520	npadvmod	times,identified
R153	T346	T340	appos	HOMER3,protein
R1530	T2524	T2522	cc	or,3
R1531	T2525	T2522	conj	more,3
R1532	T2526	T2520	punct	", ",identified
R1533	T2527	T2520	dep	Homer3,identified
R1534	T2528	T2527	punct	(,Homer3
R1535	T2529	T2527	appos	NM_011984,Homer3
R1536	T2530	T2527	punct	),Homer3
R1537	T2531	T2527	punct	", ",Homer3
R1538	T2532	T2527	conj	Dncl1,Homer3
R1539	T2533	T2532	punct	(,Dncl1
R154	T347	T340	punct	", ",protein
R1540	T2534	T2535	nmod	Dynein,chain
R1541	T2535	T2532	appos	chain,Dncl1
R1542	T2536	T2535	amod	cytoplasmic,chain
R1543	T2537	T2535	amod	light,chain
R1544	T2538	T2535	nummod	1,chain
R1545	T2539	T2535	punct	", ",chain
R1546	T2540	T2535	appos	NM_019682,chain
R1547	T2541	T2535	punct	", ",chain
R1548	T2542	T2543	advmod	also,known
R1549	T2543	T2535	acl	known,chain
R155	T348	T349	det	the,subunit
R1550	T2544	T2543	prep	as,known
R1551	T2545	T2544	pobj	Pin,as
R1552	T2546	T2545	cc	or,Pin
R1553	T2547	T2545	conj	Dlc8,Pin
R1554	T2548	T2532	punct	),Dncl1
R1555	T2549	T2532	cc	and,Dncl1
R1556	T2550	T2532	conj	Trim11,Dncl1
R1557	T2551	T2550	punct	(,Trim11
R1558	T2552	T2553	amod	Tripartite,motif
R1559	T2553	T2554	compound	motif,member
R156	T349	T340	conj	subunit,protein
R1560	T2554	T2550	appos	member,Trim11
R1561	T2555	T2554	compound	protein,member
R1562	T2556	T2554	compound	family,member
R1563	T2557	T2554	nummod	11,member
R1564	T2558	T2554	punct	", ",member
R1565	T2559	T2554	appos	NM_053168,member
R1566	T2560	T2550	punct	),Trim11
R1567	T2561	T2520	punct	.,identified
R1568	T2563	T2564	nsubjpass	Homer3,identified
R1569	T2565	T2566	punct	(,clones
R157	T350	T349	compound	dynein,subunit
R1570	T2566	T2563	parataxis	clones,Homer3
R1571	T2567	T2566	nummod	6,clones
R1572	T2568	T2566	punct	),clones
R1573	T2569	T2563	cc	and,Homer3
R1574	T2570	T2563	conj	Dncl1,Homer3
R1575	T2571	T2572	punct	(,clones
R1576	T2572	T2570	parataxis	clones,Dncl1
R1577	T2573	T2572	nummod	2,clones
R1578	T2574	T2572	punct	),clones
R1579	T2575	T2564	auxpass	were,identified
R158	T351	T349	appos	DNCL1,subunit
R1580	T2576	T2564	prep	in,identified
R1581	T2577	T2578	det	the,screen
R1582	T2578	T2576	pobj	screen,in
R1583	T2579	T2580	npadvmod	C,terminal
R1584	T2580	T2578	amod	terminal,screen
R1585	T2581	T2580	punct	-,terminal
R1586	T2582	T2578	compound	peptide,screen
R1587	T2583	T2564	punct	.,identified
R1588	T2585	T2586	nsubjpass	Homer3,identified
R1589	T2587	T2588	punct	(,clones
R159	T352	T349	punct	", ",subunit
R1590	T2588	T2585	parataxis	clones,Homer3
R1591	T2589	T2588	nummod	7,clones
R1592	T2590	T2588	punct	),clones
R1593	T2591	T2585	punct	", ",Homer3
R1594	T2592	T2585	conj	Dncl1,Homer3
R1595	T2593	T2594	punct	(,clones
R1596	T2594	T2592	parataxis	clones,Dncl1
R1597	T2595	T2594	nummod	2,clones
R1598	T2596	T2594	punct	),clones
R1599	T2597	T2592	cc	and,Dncl1
R16	T192	T191	pobj	HOMER3,with
R160	T353	T349	cc	and,subunit
R1600	T2598	T2592	conj	Trim11,Dncl1
R1601	T2599	T2600	punct	(,clones
R1602	T2600	T2598	parataxis	clones,Trim11
R1603	T2601	T2600	nummod	6,clones
R1604	T2602	T2600	punct	),clones
R1605	T2603	T2586	auxpass	were,identified
R1606	T2604	T2586	prep	in,identified
R1607	T2605	T2606	det	the,screen
R1608	T2606	T2604	pobj	screen,in
R1609	T2607	T2606	compound	PST,screen
R161	T354	T355	det	the,protein
R1610	T2608	T2606	compound	domain,screen
R1611	T2609	T2586	punct	.,identified
R1612	T2611	T2612	det	All,inserts
R1613	T2612	T2614	nsubj	inserts,were
R1614	T2613	T2612	compound	cDNA,inserts
R1615	T2615	T2614	prep	in,were
R1616	T2616	T2615	punct	-,in
R1617	T2617	T2615	pobj	frame,in
R1618	T2618	T2617	prep	with,frame
R1619	T2619	T2620	det	the,region
R162	T355	T349	conj	protein,subunit
R1620	T2620	T2618	pobj	region,with
R1621	T2621	T2620	compound	coding,region
R1622	T2622	T2620	prep	of,region
R1623	T2623	T2624	det	the,domain
R1624	T2624	T2622	pobj	domain,of
R1625	T2625	T2624	compound	pPC86,domain
R1626	T2626	T2624	compound	GAL4,domain
R1627	T2627	T2624	compound	activation,domain
R1628	T2628	T2614	punct	.,were
R1629	T2630	T2631	nsubj	None,was
R163	T356	T355	amod	tripartite,protein
R1630	T2632	T2630	prep	of,None
R1631	T2633	T2634	det	the,cDNAs
R1632	T2634	T2632	pobj	cDNAs,of
R1633	T2635	T2631	acomp	present,was
R1634	T2636	T2631	prep	in,was
R1635	T2637	T2638	det	a,list
R1636	T2638	T2636	pobj	list,in
R1637	T2639	T2638	prep	of,list
R1638	T2640	T2641	amod	known,positives
R1639	T2641	T2639	pobj	positives,of
R164	T357	T355	compound	motif,protein
R1640	T2642	T2641	amod	false,positives
R1641	T2643	T2644	punct	[,34
R1642	T2644	T2631	parataxis	34,was
R1643	T2645	T2644	punct	],34
R1644	T2646	T2631	punct	.,was
R1645	T2648	T2649	nsubj	HOMER3,is
R1646	T2650	T2651	det	a,member
R1647	T2651	T2649	attr	member,is
R1648	T2652	T2651	prep	of,member
R1649	T2653	T2654	det	the,family
R165	T358	T355	appos	TRIM11,protein
R1650	T2654	T2652	pobj	family,of
R1651	T2655	T2654	compound	HOMER,family
R1652	T2656	T2654	prep	of,family
R1653	T2657	T2658	amod	neuronal,proteins
R1654	T2658	T2656	pobj	proteins,of
R1655	T2659	T2660	amod	post-synaptic,density
R1656	T2660	T2658	nmod	density,proteins
R1657	T2661	T2660	punct	(,density
R1658	T2662	T2660	appos	PSD,density
R1659	T2663	T2658	punct	),proteins
R166	T359	T336	punct	.,identified
R1660	T2664	T2665	punct	[,35
R1661	T2665	T2649	parataxis	35,is
R1662	T2666	T2665	punct	],35
R1663	T2667	T2649	punct	.,is
R1664	T2669	T2670	nsubj	DNCL1,is
R1665	T2671	T2672	det	a,subunit
R1666	T2672	T2670	attr	subunit,is
R1667	T2673	T2672	prep	of,subunit
R1668	T2674	T2675	nummod	two,complexes
R1669	T2675	T2673	pobj	complexes,of
R167	T361	T362	nummod	Three,mutations
R1670	T2676	T2675	compound	motor,complexes
R1671	T2677	T2675	compound	protein,complexes
R1672	T2678	T2675	punct	", ",complexes
R1673	T2679	T2675	appos	dynein,complexes
R1674	T2680	T2679	cc	and,dynein
R1675	T2681	T2682	compound	myosin,Va
R1676	T2682	T2679	conj	Va,dynein
R1677	T2683	T2682	punct	-,Va
R1678	T2684	T2679	punct	", ",dynein
R1679	T2685	T2686	dep	both,involved
R168	T362	T367	nsubj	mutations,reduced
R1680	T2686	T2679	relcl	involved,dynein
R1681	T2687	T2685	prep	of,both
R1682	T2688	T2687	pobj	which,of
R1683	T2689	T2686	auxpass	are,involved
R1684	T2690	T2686	prep	in,involved
R1685	T2691	T2692	amod	intracellular,trafficking
R1686	T2692	T2690	pobj	trafficking,in
R1687	T2693	T2692	prep	of,trafficking
R1688	T2694	T2693	pobj	proteins,of
R1689	T2695	T2694	cc	and,proteins
R169	T363	T364	npadvmod	C,terminal
R1690	T2696	T2694	conj	organelles,proteins
R1691	T2697	T2686	prep	in,involved
R1692	T2698	T2697	pobj	neurons,in
R1693	T2699	T2700	punct	[,37
R1694	T2700	T2670	parataxis	37,is
R1695	T2701	T2700	nummod	36,37
R1696	T2702	T2700	punct	",",37
R1697	T2703	T2700	punct	],37
R1698	T2704	T2670	punct	.,is
R1699	T2706	T2707	nsubj	TRIM11,is
R17	T193	T192	punct	", ",HOMER3
R170	T364	T362	amod	terminal,mutations
R1700	T2708	T2709	det	a,member
R1701	T2709	T2707	attr	member,is
R1702	T2710	T2709	prep	of,member
R1703	T2711	T2712	det	the,family
R1704	T2712	T2710	pobj	family,of
R1705	T2713	T2714	amod	tripartite,motif
R1706	T2714	T2712	compound	motif,family
R1707	T2715	T2712	compound	protein,family
R1708	T2716	T2707	cc	and,is
R1709	T2717	T2707	conj	contains,is
R171	T365	T364	punct	-,terminal
R1710	T2718	T2719	det	a,finger
R1711	T2719	T2717	dobj	finger,contains
R1712	T2720	T2719	compound	RING,finger
R1713	T2721	T2719	punct	", ",finger
R1714	T2722	T2723	det	a,finger
R1715	T2723	T2719	conj	finger,finger
R1716	T2724	T2725	compound	B,box
R1717	T2725	T2723	compound	box,finger
R1718	T2726	T2725	punct	-,box
R1719	T2727	T2723	compound	zinc,finger
R172	T366	T362	compound	PAX6,mutations
R1720	T2728	T2723	punct	", ",finger
R1721	T2729	T2730	det	a,domain
R1722	T2730	T2723	conj	domain,finger
R1723	T2731	T2732	amod	coiled,coil
R1724	T2732	T2730	compound	coil,domain
R1725	T2733	T2730	cc	and,domain
R1726	T2734	T2735	det	a,domain
R1727	T2735	T2730	conj	domain,domain
R1728	T2736	T2735	compound	B30.2,domain
R1729	T2737	T2738	punct	[,38
R173	T368	T362	punct	", ",mutations
R1730	T2738	T2717	parataxis	38,contains
R1731	T2739	T2738	punct	],38
R1732	T2740	T2707	punct	.,is
R1733	T2742	T2743	det	The,significance
R1734	T2743	T2745	nsubjpass	significance,discussed
R1735	T2744	T2743	amod	possible,significance
R1736	T2746	T2743	prep	of,significance
R1737	T2747	T2748	det	the,interactions
R1738	T2748	T2746	pobj	interactions,of
R1739	T2749	T2748	prep	between,interactions
R174	T369	T370	advmod	previously,identified
R1740	T2750	T2751	det	these,proteins
R1741	T2751	T2749	pobj	proteins,between
R1742	T2752	T2751	cc	and,proteins
R1743	T2753	T2751	conj	PAX6,proteins
R1744	T2754	T2745	auxpass	is,discussed
R1745	T2755	T2745	advmod	below,discussed
R1746	T2756	T2745	punct	.,discussed
R1747	T2839	T2840	amod	Semi-quantitative,PCR
R1748	T2842	T2843	aux	To,check
R1749	T2843	T2844	advcl	check,performed
R175	T370	T362	acl	identified,mutations
R1750	T2845	T2846	mark	that,identified
R1751	T2846	T2843	ccomp	identified,check
R1752	T2847	T2848	det	the,clones
R1753	T2848	T2846	nsubjpass	clones,identified
R1754	T2849	T2848	nmod	Homer3,clones
R1755	T2850	T2849	punct	", ",Homer3
R1756	T2851	T2849	conj	Dncl1,Homer3
R1757	T2852	T2851	cc	and,Dncl1
R1758	T2853	T2851	conj	Trim11,Dncl1
R1759	T2854	T2846	auxpass	were,identified
R176	T371	T370	prep	in,identified
R1760	T2855	T2846	neg	not,identified
R1761	T2856	T2857	amod	multiple,times
R1762	T2857	T2846	npadvmod	times,identified
R1763	T2858	T2859	advmod	solely,are
R1764	T2859	T2846	advcl	are,identified
R1765	T2860	T2859	mark	because,are
R1766	T2861	T2859	nsubj	they,are
R1767	T2862	T2863	advmod	highly,abundant
R1768	T2863	T2859	acomp	abundant,are
R1769	T2864	T2859	prep	in,are
R177	T372	T371	pobj	patients,in
R1770	T2865	T2866	det	the,library
R1771	T2866	T2864	pobj	library,in
R1772	T2867	T2844	punct	", ",performed
R1773	T2868	T2844	nsubj	we,performed
R1774	T2869	T2870	det	a,assay
R1775	T2870	T2844	dobj	assay,performed
R1776	T2871	T2870	amod	semi-quantitative,assay
R1777	T2872	T2870	compound	PCR,assay
R1778	T2873	T2844	punct	.,performed
R1779	T2875	T2876	nsubj	We,compared
R178	T373	T372	prep	with,patients
R1780	T2877	T2878	det	the,abundance
R1781	T2878	T2876	dobj	abundance,compared
R1782	T2879	T2878	amod	relative,abundance
R1783	T2880	T2878	prep	of,abundance
R1784	T2881	T2880	pobj	Homer3,of
R1785	T2882	T2881	punct	", ",Homer3
R1786	T2883	T2881	conj	Dncl1,Homer3
R1787	T2884	T2883	punct	", ",Dncl1
R1788	T2885	T2883	conj	Trim11,Dncl1
R1789	T2886	T2885	cc	and,Trim11
R179	T374	T375	compound	eye,malformations
R1790	T2887	T2885	conj	Pax6,Trim11
R1791	T2888	T2876	prep	with,compared
R1792	T2889	T2888	pobj	Gapdh,with
R1793	T2890	T2889	cc	and,Gapdh
R1794	T2891	T2889	conj	Atp5a1,Gapdh
R1795	T2892	T2876	punct	.,compared
R1796	T2894	T2895	nsubj	Gapdh,show
R1797	T2896	T2894	cc	and,Gapdh
R1798	T2897	T2894	conj	Atp5a1,Gapdh
R1799	T2898	T2894	appos	both,Gapdh
R18	T194	T192	conj	DNCL1,HOMER3
R180	T375	T373	pobj	malformations,with
R1800	T2899	T2900	amod	strong,expression
R1801	T2900	T2895	dobj	expression,show
R1802	T2901	T2900	amod	constitutive,expression
R1803	T2902	T2895	prep	in,show
R1804	T2903	T2904	det	a,variety
R1805	T2904	T2902	pobj	variety,in
R1806	T2905	T2904	prep	of,variety
R1807	T2906	T2905	pobj	tissues,of
R1808	T2907	T2906	prep	including,tissues
R1809	T2908	T2909	det	the,brain
R181	T376	T362	punct	", ",mutations
R1810	T2909	T2907	pobj	brain,including
R1811	T2910	T2911	punct	[,30
R1812	T2911	T2895	parataxis	30,show
R1813	T2912	T2911	nummod	29,30
R1814	T2913	T2911	punct	",",30
R1815	T2914	T2911	punct	],30
R1816	T2915	T2895	punct	.,show
R1817	T2917	T2918	nsubjpass	Homer3,amplified
R1818	T2919	T2917	punct	", ",Homer3
R1819	T2920	T2917	conj	Dncl1,Homer3
R182	T377	T362	appos	all,mutations
R1820	T2921	T2920	punct	", ",Dncl1
R1821	T2922	T2920	conj	Trim11,Dncl1
R1822	T2923	T2922	cc	and,Trim11
R1823	T2924	T2922	conj	Pax6,Trim11
R1824	T2925	T2918	auxpass	were,amplified
R1825	T2926	T2918	advmod	only,amplified
R1826	T2927	T2918	advmod	strongly,amplified
R1827	T2928	T2918	prep	after,amplified
R1828	T2929	T2930	nummod	35,cycles
R1829	T2930	T2928	pobj	cycles,after
R183	T378	T367	cc	or,reduced
R1830	T2931	T2930	prep	of,cycles
R1831	T2932	T2931	pobj	PCR,of
R1832	T2933	T2934	punct	(,Figure
R1833	T2934	T2918	parataxis	Figure,amplified
R1834	T2935	T2934	nummod	2,Figure
R1835	T2936	T2934	punct	),Figure
R1836	T2937	T2918	cc	and,amplified
R1837	T2938	T2918	conj	were,amplified
R1838	T2939	T2938	advmod	therefore,were
R1839	T2940	T2938	acomp	present,were
R184	T379	T367	conj	abolished,reduced
R1840	T2941	T2940	prep	at,present
R1841	T2942	T2943	advmod	relatively,low
R1842	T2943	T2944	amod	low,levels
R1843	T2944	T2941	pobj	levels,at
R1844	T2945	T2938	prep	compared,were
R1845	T2946	T2945	prep	to,compared
R1846	T2947	T2946	pobj	Gapdh,to
R1847	T2948	T2947	punct	(,Gapdh
R1848	T2949	T2947	acl	amplified,Gapdh
R1849	T2950	T2949	advmod	strongly,amplified
R185	T380	T381	det	the,interactions
R1850	T2951	T2949	prep	after,amplified
R1851	T2952	T2953	nummod	25,cycles
R1852	T2953	T2951	pobj	cycles,after
R1853	T2954	T2947	punct	),Gapdh
R1854	T2955	T2947	cc	and,Gapdh
R1855	T2956	T2947	conj	Atp5a1,Gapdh
R1856	T2957	T2956	punct	(,Atp5a1
R1857	T2958	T2956	acl	amplified,Atp5a1
R1858	T2959	T2958	advmod	strongly,amplified
R1859	T2960	T2958	prep	after,amplified
R186	T381	T379	dobj	interactions,abolished
R1860	T2961	T2962	nummod	30,cycles
R1861	T2962	T2960	pobj	cycles,after
R1862	T2963	T2964	punct	;,Figure
R1863	T2964	T2938	parataxis	Figure,were
R1864	T2965	T2964	nummod	2,Figure
R1865	T2966	T2964	punct	),Figure
R1866	T2967	T2918	punct	.,amplified
R1867	T2969	T2970	nsubj	We,concluded
R1868	T2971	T2972	mark	that,were
R1869	T2972	T2970	ccomp	were,concluded
R187	T382	T367	punct	.,reduced
R1870	T2973	T2974	nmod	Homer3,clones
R1871	T2974	T2972	nsubj	clones,were
R1872	T2975	T2973	punct	", ",Homer3
R1873	T2976	T2973	conj	Dncl1,Homer3
R1874	T2977	T2976	cc	and,Dncl1
R1875	T2978	T2976	conj	Trim11,Dncl1
R1876	T2979	T2972	neg	not,were
R1877	T2980	T2981	advmod	highly,abundant
R1878	T2981	T2972	acomp	abundant,were
R1879	T2982	T2972	prep	in,were
R188	T386	T387	poss	Our,data
R1880	T2983	T2984	det	the,library
R1881	T2984	T2982	pobj	library,in
R1882	T2985	T2970	punct	.,concluded
R1883	T2987	T2988	nsubj	This,is
R1884	T2989	T2988	acomp	consistent,is
R1885	T2990	T2989	prep	with,consistent
R1886	T2991	T2992	det	the,idea
R1887	T2992	T2990	pobj	idea,with
R1888	T2993	T2994	mark	that,pulled
R1889	T2994	T2992	acl	pulled,idea
R189	T387	T389	nsubj	data,suggest
R1890	T2995	T2994	nsubjpass	they,pulled
R1891	T2996	T2994	auxpass	were,pulled
R1892	T2997	T2994	prt	out,pulled
R1893	T2998	T2999	mark	because,interact
R1894	T2999	T2994	advcl	interact,pulled
R1895	T3000	T3001	det	the,proteins
R1896	T3001	T2999	nsubj	proteins,interact
R1897	T3002	T3001	amod	encoded,proteins
R1898	T3003	T2999	advmod	specifically,interact
R1899	T3004	T2999	prep	with,interact
R19	T195	T194	cc	and,DNCL1
R190	T388	T387	amod	preliminary,data
R1900	T3005	T3006	det	the,peptide
R1901	T3006	T3004	pobj	peptide,with
R1902	T3007	T3008	npadvmod	C,terminal
R1903	T3008	T3006	amod	terminal,peptide
R1904	T3009	T3008	punct	-,terminal
R1905	T3010	T3006	cc	or,peptide
R1906	T3011	T3012	compound	PST,domain
R1907	T3012	T3006	conj	domain,peptide
R1908	T3013	T3012	prep	of,domain
R1909	T3014	T3013	pobj	PAX6,of
R191	T390	T391	mark	that,interacts
R1910	T3015	T2988	punct	.,is
R1911	T3382	T3383	nmod	Yeast,interactions
R1912	T3384	T3385	nummod	two,hybrid
R1913	T3385	T3383	nmod	hybrid,interactions
R1914	T3386	T3385	punct	-,hybrid
R1915	T3387	T3383	amod	pairwise,interactions
R1916	T3389	T3390	prep	By,identified
R1917	T3391	T3392	compound	library,screening
R1918	T3392	T3389	pobj	screening,By
R1919	T3393	T3390	nsubj	we,identified
R192	T391	T389	ccomp	interacts,suggest
R1920	T3394	T3395	nummod	two,proteins
R1921	T3395	T3390	dobj	proteins,identified
R1922	T3396	T3395	punct	(,proteins
R1923	T3397	T3395	appos	HOMER3,proteins
R1924	T3398	T3397	cc	and,HOMER3
R1925	T3399	T3397	conj	DNCL1,HOMER3
R1926	T3400	T3395	punct	),proteins
R1927	T3401	T3402	dep	that,interact
R1928	T3402	T3395	relcl	interact,proteins
R1929	T3403	T3402	prep	with,interact
R193	T392	T391	nsubj	PAX6,interacts
R1930	T3404	T3405	det	the,peptide
R1931	T3405	T3403	pobj	peptide,with
R1932	T3406	T3407	npadvmod	C,terminal
R1933	T3407	T3405	amod	terminal,peptide
R1934	T3408	T3407	punct	-,terminal
R1935	T3409	T3405	cc	and,peptide
R1936	T3410	T3411	nummod	three,proteins
R1937	T3411	T3405	conj	proteins,peptide
R1938	T3412	T3411	punct	(,proteins
R1939	T3413	T3411	appos	HOMER3,proteins
R194	T393	T391	prep	with,interacts
R1940	T3414	T3413	punct	", ",HOMER3
R1941	T3415	T3413	conj	DNCL1,HOMER3
R1942	T3416	T3415	cc	and,DNCL1
R1943	T3417	T3415	conj	TRIM11,DNCL1
R1944	T3418	T3411	punct	),proteins
R1945	T3419	T3420	dep	that,interact
R1946	T3420	T3411	relcl	interact,proteins
R1947	T3421	T3420	prep	with,interact
R1948	T3422	T3423	det	the,domain
R1949	T3423	T3421	pobj	domain,with
R195	T394	T393	pobj	HOMER3,with
R1950	T3424	T3423	amod	whole,domain
R1951	T3425	T3423	compound	PST,domain
R1952	T3426	T3390	punct	.,identified
R1953	T3428	T3429	nsubj	This,suggests
R1954	T3430	T3431	mark	that,interact
R1955	T3431	T3429	ccomp	interact,suggests
R1956	T3432	T3431	nsubj	HOMER3,interact
R1957	T3433	T3432	cc	and,HOMER3
R1958	T3434	T3432	conj	DNCL1,HOMER3
R1959	T3435	T3436	advmod	specifically,with
R196	T395	T394	punct	", ",HOMER3
R1960	T3436	T3431	prep	with,interact
R1961	T3437	T3438	det	the,peptide
R1962	T3438	T3436	pobj	peptide,with
R1963	T3439	T3440	npadvmod	C,terminal
R1964	T3440	T3438	amod	terminal,peptide
R1965	T3441	T3440	punct	-,terminal
R1966	T3442	T3443	mark	while,interacts
R1967	T3443	T3431	advcl	interacts,interact
R1968	T3444	T3443	nsubj	TRIM11,interacts
R1969	T3445	T3443	prep	with,interacts
R197	T396	T394	conj	DNCL1,HOMER3
R1970	T3446	T3447	det	a,part
R1971	T3447	T3445	pobj	part,with
R1972	T3448	T3449	advmod	more,terminal
R1973	T3449	T3447	amod	terminal,part
R1974	T3450	T3449	npadvmod	N,terminal
R1975	T3451	T3449	punct	-,terminal
R1976	T3452	T3447	prep	of,part
R1977	T3453	T3454	det	the,domain
R1978	T3454	T3452	pobj	domain,of
R1979	T3455	T3454	compound	PST,domain
R198	T397	T396	cc	and,DNCL1
R1980	T3456	T3429	punct	.,suggests
R1981	T3458	T3459	nsubj	We,conducted
R1982	T3460	T3461	amod	pairwise,tests
R1983	T3461	T3459	dobj	tests,conducted
R1984	T3462	T3461	prep	between,tests
R1985	T3463	T3464	amod	specific,constructs
R1986	T3464	T3462	pobj	constructs,between
R1987	T3465	T3466	aux	to,confirm
R1988	T3466	T3459	advcl	confirm,conducted
R1989	T3467	T3468	det	the,interactions
R199	T398	T396	conj	TRIM11,DNCL1
R1990	T3468	T3466	dobj	interactions,confirm
R1991	T3469	T3468	acl	identified,interactions
R1992	T3470	T3469	prep	in,identified
R1993	T3471	T3472	det	the,screen
R1994	T3472	T3470	pobj	screen,in
R1995	T3473	T3472	compound	library,screen
R1996	T3474	T3466	cc	and,confirm
R1997	T3475	T3476	aux	to,investigate
R1998	T3476	T3466	conj	investigate,confirm
R1999	T3477	T3476	advmod	further,investigate
R2	T176	T178	dep	screen,disrupt
R20	T196	T194	conj	TRIM11,DNCL1
R200	T399	T389	punct	.,suggest
R2000	T3478	T3479	det	the,interaction
R2001	T3479	T3476	dobj	interaction,investigate
R2002	T3480	T3479	prep	between,interaction
R2003	T3481	T3480	pobj	PAX6,between
R2004	T3482	T3481	cc	and,PAX6
R2005	T3483	T3481	conj	HOMER3,PAX6
R2006	T3484	T3481	punct	", ",PAX6
R2007	T3485	T3481	appos	DNCL1,PAX6
R2008	T3486	T3485	cc	and,DNCL1
R2009	T3487	T3485	conj	TRIM11,DNCL1
R201	T401	T402	nsubj	We,propose
R2010	T3488	T3459	punct	.,conducted
R2011	T3490	T3491	det	The,clones
R2012	T3491	T3495	nsubj	clones,were
R2013	T3492	T3491	nmod	Dncl1,clones
R2014	T3493	T3492	cc	and,Dncl1
R2015	T3494	T3492	conj	Trim11,Dncl1
R2016	T3496	T3497	dep	that,pulled
R2017	T3497	T3491	relcl	pulled,clones
R2018	T3498	T3497	auxpass	were,pulled
R2019	T3499	T3497	prep	out,pulled
R202	T403	T404	mark	that,is
R2020	T3500	T3499	prep	of,out
R2021	T3501	T3502	det	the,library
R2022	T3502	T3500	pobj	library,of
R2023	T3503	T3504	amod	full,length
R2024	T3504	T3495	attr	length,were
R2025	T3505	T3504	punct	-,length
R2026	T3506	T3495	punct	", ",were
R2027	T3507	T3495	cc	but,were
R2028	T3508	T3509	det	the,cDNAs
R2029	T3509	T3511	nsubj	cDNAs,lacked
R203	T404	T402	advcl	is,propose
R2030	T3510	T3509	compound	Homer3,cDNAs
R2031	T3511	T3495	conj	lacked,were
R2032	T3512	T3513	det	the,acids
R2033	T3513	T3511	dobj	acids,lacked
R2034	T3514	T3515	npadvmod	N,terminal
R2035	T3515	T3513	amod	terminal,acids
R2036	T3516	T3515	punct	-,terminal
R2037	T3517	T3513	nummod	70,acids
R2038	T3518	T3513	compound	amino,acids
R2039	T3519	T3511	punct	.,lacked
R204	T405	T406	det	the,interaction
R2040	T3521	T3522	det	The,region
R2041	T3522	T3525	nsubjpass	region,inserted
R2042	T3523	T3522	amod	missing,region
R2043	T3524	T3522	compound	coding,region
R2044	T3526	T3525	auxpass	was,inserted
R2045	T3527	T3525	prep	into,inserted
R2046	T3528	T3529	det	the,cDNA
R2047	T3529	T3527	pobj	cDNA,into
R2048	T3530	T3529	amod	truncated,cDNA
R2049	T3531	T3529	compound	Homer3,cDNA
R205	T406	T404	nsubj	interaction,is
R2050	T3532	T3533	aux	to,give
R2051	T3533	T3525	advcl	give,inserted
R2052	T3534	T3535	det	a,construct
R2053	T3535	T3533	dobj	construct,give
R2054	T3536	T3537	amod	full,length
R2055	T3537	T3535	compound	length,construct
R2056	T3538	T3537	punct	-,length
R2057	T3539	T3535	compound	expression,construct
R2058	T3540	T3541	punct	(,see
R2059	T3541	T3525	parataxis	see,inserted
R206	T407	T406	prep	of,interaction
R2060	T3542	T3541	dobj	Methods,see
R2061	T3543	T3541	punct	),see
R2062	T3544	T3525	punct	.,inserted
R2063	T3546	T3547	amod	Pairwise,interactions
R2064	T3547	T3548	nsubjpass	interactions,carried
R2065	T3549	T3548	auxpass	were,carried
R2066	T3550	T3548	prt	out,carried
R2067	T3551	T3548	prep	with,carried
R2068	T3552	T3553	preconj	both,clones
R2069	T3553	T3551	pobj	clones,with
R207	T408	T407	pobj	PAX6,of
R2070	T3554	T3553	det	the,clones
R2071	T3555	T3556	amod	full,length
R2072	T3556	T3553	nmod	length,clones
R2073	T3557	T3556	punct	-,length
R2074	T3558	T3556	cc	and,length
R2075	T3559	T3556	conj	truncated,length
R2076	T3560	T3553	compound	Homer3,clones
R2077	T3561	T3548	punct	.,carried
R2078	T3563	T3564	nsubj	We,confirmed
R2079	T3565	T3566	mark	that,interacts
R208	T409	T406	prep	with,interaction
R2080	T3566	T3564	ccomp	interacts,confirmed
R2081	T3567	T3568	det	the,domain
R2082	T3568	T3566	nsubj	domain,interacts
R2083	T3569	T3568	amod	whole,domain
R2084	T3570	T3571	compound	PAX6,PST
R2085	T3571	T3568	compound	PST,domain
R2086	T3572	T3566	prep	with,interacts
R2087	T3573	T3572	pobj	HOMER3,with
R2088	T3574	T3575	punct	(,constructs
R2089	T3575	T3573	parataxis	constructs,HOMER3
R209	T410	T409	pobj	HOMER3,with
R2090	T3576	T3577	amod	full,length
R2091	T3577	T3575	nmod	length,constructs
R2092	T3578	T3577	punct	-,length
R2093	T3579	T3577	cc	and,length
R2094	T3580	T3577	conj	truncated,length
R2095	T3581	T3575	punct	),constructs
R2096	T3582	T3573	punct	", ",HOMER3
R2097	T3583	T3573	conj	TRIM11,HOMER3
R2098	T3584	T3583	cc	and,TRIM11
R2099	T3585	T3583	conj	DNCL1,TRIM11
R21	T201	T202	det	The,protein
R210	T411	T410	cc	and,HOMER3
R2100	T3586	T3566	punct	", ",interacts
R2101	T3587	T3588	mark	as,activated
R2102	T3588	T3566	advcl	activated,interacts
R2103	T3589	T3590	det	all,genes
R2104	T3590	T3588	nsubjpass	genes,activated
R2105	T3591	T3590	nummod	three,genes
R2106	T3592	T3590	compound	reporter,genes
R2107	T3593	T3588	auxpass	were,activated
R2108	T3594	T3588	advmod	strongly,activated
R2109	T3595	T3588	prep	in,activated
R211	T412	T410	conj	DNCL1,HOMER3
R2110	T3596	T3597	amod	pairwise,tests
R2111	T3597	T3595	pobj	tests,in
R2112	T3598	T3599	punct	(,Table
R2113	T3599	T3564	parataxis	Table,confirmed
R2114	T3600	T3599	dep	Figure,Table
R2115	T3601	T3600	nummod	3,Figure
R2116	T3602	T3599	punct	;,Table
R2117	T3603	T3599	nummod	1,Table
R2118	T3604	T3599	punct	),Table
R2119	T3605	T3564	punct	.,confirmed
R212	T413	T414	det	a,mechanism
R2120	T3607	T3608	prep	In,confirmed
R2121	T3609	T3607	pobj	contrast,In
R2122	T3610	T3611	det	the,interaction
R2123	T3611	T3608	nsubjpass	interaction,confirmed
R2124	T3612	T3611	prep	between,interaction
R2125	T3613	T3614	det	the,peptide
R2126	T3614	T3612	pobj	peptide,between
R2127	T3615	T3616	npadvmod	C,terminal
R2128	T3616	T3614	amod	terminal,peptide
R2129	T3617	T3616	punct	-,terminal
R213	T414	T404	attr	mechanism,is
R2130	T3618	T3614	cc	and,peptide
R2131	T3619	T3614	conj	HOMER3,peptide
R2132	T3620	T3619	cc	or,HOMER3
R2133	T3621	T3619	conj	DNCL1,HOMER3
R2134	T3622	T3608	aux	could,confirmed
R2135	T3623	T3608	neg	not,confirmed
R2136	T3624	T3608	auxpass	be,confirmed
R2137	T3625	T3608	prep	with,confirmed
R2138	T3626	T3627	amod	pairwise,tests
R2139	T3627	T3625	pobj	tests,with
R214	T415	T416	prep	by,lead
R2140	T3628	T3629	punct	(,Table
R2141	T3629	T3608	parataxis	Table,confirmed
R2142	T3630	T3629	nummod	1,Table
R2143	T3631	T3629	punct	),Table
R2144	T3632	T3608	punct	.,confirmed
R2145	T3634	T3635	advmod	Occasionally,observed
R2146	T3635	T3651	ccomp	observed,observed
R2147	T3636	T3635	punct	", ",observed
R2148	T3637	T3638	amod	partial,suppression
R2149	T3638	T3635	nsubjpass	suppression,observed
R215	T416	T414	relcl	lead,mechanism
R2150	T3639	T3638	prep	of,suppression
R2151	T3640	T3639	pobj	growth,of
R2152	T3641	T3638	prep	on,suppression
R2153	T3642	T3641	pobj	plates,on
R2154	T3643	T3642	acl	containing,plates
R2155	T3644	T3645	advmod	5,orotic
R2156	T3645	T3649	amod	orotic,acid
R2157	T3646	T3645	punct	-,orotic
R2158	T3647	T3645	amod	fluoro,orotic
R2159	T3648	T3645	punct	-,orotic
R216	T417	T415	pobj	which,by
R2160	T3649	T3643	dobj	acid,containing
R2161	T3650	T3635	auxpass	was,observed
R2162	T3652	T3635	punct	", ",observed
R2163	T3653	T3635	advcl	indicating,observed
R2164	T3654	T3655	amod	low,level
R2165	T3655	T3657	compound	level,activation
R2166	T3656	T3655	punct	-,level
R2167	T3657	T3653	dobj	activation,indicating
R2168	T3658	T3657	prep	of,activation
R2169	T3659	T3660	det	the,reporter
R217	T418	T419	amod	synaptic,activation
R2170	T3660	T3658	pobj	reporter,of
R2171	T3661	T3660	compound	URA3,reporter
R2172	T3662	T3651	punct	;,observed
R2173	T3663	T3651	advmod	however,observed
R2174	T3664	T3665	nmod	HIS3,activation
R2175	T3665	T3651	nsubjpass	activation,observed
R2176	T3666	T3664	cc	and,HIS3
R2177	T3667	T3664	conj	LacZ,HIS3
R2178	T3668	T3651	auxpass	were,observed
R2179	T3669	T3651	neg	not,observed
R218	T419	T416	nsubj	activation,lead
R2180	T3670	T3651	punct	.,observed
R2181	T3672	T3673	det	The,reasons
R2182	T3673	T3674	nsubj	reasons,are
R2183	T3675	T3673	prep	for,reasons
R2184	T3676	T3675	pobj	this,for
R2185	T3677	T3674	neg	not,are
R2186	T3678	T3674	acomp	clear,are
R2187	T3679	T3674	punct	", ",are
R2188	T3680	T3681	mark	although,be
R2189	T3681	T3674	advcl	be,are
R219	T420	T416	aux	could,lead
R2190	T3682	T3681	nsubj	it,be
R2191	T3683	T3681	aux	may,be
R2192	T3684	T3685	mark	that,were
R2193	T3685	T3681	ccomp	were,be
R2194	T3686	T3687	det	the,tests
R2195	T3687	T3685	nsubj	tests,were
R2196	T3688	T3687	amod	pairwise,tests
R2197	T3689	T3685	prep	of,were
R2198	T3690	T3691	amod	sub-optimal,sensitivity
R2199	T3691	T3689	pobj	sensitivity,of
R22	T202	T204	nsubj	protein,is
R220	T421	T416	prep	to,lead
R2200	T3692	T3685	prep	compared,were
R2201	T3693	T3692	prep	to,compared
R2202	T3694	T3695	det	the,screen
R2203	T3695	T3693	pobj	screen,to
R2204	T3696	T3695	compound	library,screen
R2205	T3697	T3674	punct	.,are
R2206	T3699	T3700	advmod	However,were
R2207	T3701	T3700	nsubj	we,were
R2208	T3702	T3700	acomp	able,were
R2209	T3703	T3704	aux	to,confirm
R221	T422	T421	pobj	changes,to
R2210	T3704	T3702	xcomp	confirm,able
R2211	T3705	T3706	mark	that,is
R2212	T3706	T3704	ccomp	is,confirm
R2213	T3707	T3708	det	the,peptide
R2214	T3708	T3706	nsubj	peptide,is
R2215	T3709	T3710	npadvmod	C,terminal
R2216	T3710	T3708	amod	terminal,peptide
R2217	T3711	T3710	punct	-,terminal
R2218	T3712	T3706	acomp	important,is
R2219	T3713	T3712	prep	for,important
R222	T423	T422	prep	in,changes
R2220	T3714	T3715	det	the,interaction
R2221	T3715	T3713	pobj	interaction,for
R2222	T3716	T3715	prep	with,interaction
R2223	T3717	T3716	pobj	HOMER3,with
R2224	T3718	T3717	cc	and,HOMER3
R2225	T3719	T3717	conj	DNCL1,HOMER3
R2226	T3720	T3721	mark	because,abolished
R2227	T3721	T3706	advcl	abolished,is
R2228	T3722	T3721	nsubjpass	interaction,abolished
R2229	T3723	T3722	prep	with,interaction
R223	T424	T425	amod	neuronal,activity
R2230	T3724	T3725	det	the,construct
R2231	T3725	T3723	pobj	construct,with
R2232	T3726	T3727	compound	PAX6PST,CT
R2233	T3727	T3725	compound	CT,construct
R2234	T3728	T3727	punct	-,CT
R2235	T3729	T3725	punct	", ",construct
R2236	T3730	T3731	dep	which,lacks
R2237	T3731	T3725	relcl	lacks,construct
R2238	T3732	T3733	det	the,acids
R2239	T3733	T3731	dobj	acids,lacks
R224	T425	T423	pobj	activity,in
R2240	T3734	T3733	amod	final,acids
R2241	T3735	T3733	nummod	32,acids
R2242	T3736	T3733	compound	amino,acids
R2243	T3737	T3721	punct	", ",abolished
R2244	T3738	T3721	auxpass	was,abolished
R2245	T3739	T3721	advmod	completely,abolished
R2246	T3740	T3741	punct	(,Table
R2247	T3741	T3700	parataxis	Table,were
R2248	T3742	T3741	dep	Figure,Table
R2249	T3743	T3742	nummod	3,Figure
R225	T426	T425	amod	transcriptional,activity
R2250	T3744	T3741	punct	", ",Table
R2251	T3745	T3741	nummod	1,Table
R2252	T3746	T3741	punct	),Table
R2253	T3747	T3700	punct	.,were
R2254	T3749	T3750	nsubj	TRIM11,interacted
R2255	T3751	T3752	advmod	equally,well
R2256	T3752	T3750	advmod	well,interacted
R2257	T3753	T3750	prep	with,interacted
R2258	T3754	T3753	pobj	PAX6PST,with
R2259	T3755	T3754	cc	and,PAX6PST
R226	T427	T404	punct	", ",is
R2260	T3756	T3757	compound	PAX6PST,CT
R2261	T3757	T3754	conj	CT,PAX6PST
R2262	T3758	T3757	punct	-,CT
R2263	T3759	T3760	punct	(,Table
R2264	T3760	T3750	parataxis	Table,interacted
R2265	T3761	T3760	dep	Figure,Table
R2266	T3762	T3761	nummod	3,Figure
R2267	T3763	T3760	punct	", ",Table
R2268	T3764	T3760	nummod	1,Table
R2269	T3765	T3760	punct	),Table
R227	T428	T404	cc	and,is
R2270	T3766	T3750	punct	.,interacted
R2271	T3768	T3769	nsubj	This,is
R2272	T3770	T3769	acomp	consistent,is
R2273	T3771	T3770	prep	with,consistent
R2274	T3772	T3773	det	the,screens
R2275	T3773	T3771	pobj	screens,with
R2276	T3774	T3773	compound	library,screens
R2277	T3775	T3776	prep	in,isolated
R2278	T3776	T3773	relcl	isolated,screens
R2279	T3777	T3775	pobj	which,in
R228	T429	T430	mark	that,explained
R2280	T3778	T3776	nsubjpass	TRIM11,isolated
R2281	T3779	T3776	auxpass	was,isolated
R2282	T3780	T3776	prep	with,isolated
R2283	T3781	T3782	det	the,domain
R2284	T3782	T3780	pobj	domain,with
R2285	T3783	T3782	compound	PST,domain
R2286	T3784	T3780	cc	but,with
R2287	T3785	T3784	neg	not,but
R2288	T3786	T3780	conj	with,with
R2289	T3787	T3788	det	the,peptide
R229	T430	T404	conj	explained,is
R2290	T3788	T3786	pobj	peptide,with
R2291	T3789	T3790	npadvmod	C,terminal
R2292	T3790	T3788	amod	terminal,peptide
R2293	T3791	T3790	punct	-,terminal
R2294	T3792	T3769	cc	and,is
R2295	T3793	T3769	conj	supports,is
R2296	T3794	T3795	det	the,idea
R2297	T3795	T3793	dobj	idea,supports
R2298	T3796	T3797	mark	that,is
R2299	T3797	T3795	acl	is,idea
R23	T203	T202	compound	PAX6,protein
R230	T431	T430	nsubjpass	some,explained
R2300	T3798	T3799	det	the,peptide
R2301	T3799	T3797	nsubj	peptide,is
R2302	T3800	T3801	npadvmod	C,terminal
R2303	T3801	T3799	amod	terminal,peptide
R2304	T3802	T3801	punct	-,terminal
R2305	T3803	T3797	neg	not,is
R2306	T3804	T3797	acomp	important,is
R2307	T3805	T3804	prep	for,important
R2308	T3806	T3807	det	the,interaction
R2309	T3807	T3805	pobj	interaction,for
R231	T432	T431	prep	of,some
R2310	T3808	T3807	prep	between,interaction
R2311	T3809	T3808	pobj	PAX6,between
R2312	T3810	T3809	cc	and,PAX6
R2313	T3811	T3809	conj	TRIM11,PAX6
R2314	T3812	T3769	punct	.,is
R2315	T3818	T3819	nummod	v,in
R2316	T3829	T3830	advcl	ed,investigated
R2317	T3831	T3832	mark	that,interact
R2318	T3832	T3829	ccomp	interact,ed
R2319	T3833	T3832	nsubj	HOMER3,interact
R232	T433	T434	det	the,anomalies
R2320	T3834	T3833	punct	", ",HOMER3
R2321	T3835	T3833	conj	DNCL1,HOMER3
R2322	T3836	T3835	cc	and,DNCL1
R2323	T3837	T3835	conj	TRIM11,DNCL1
R2324	T3838	T3832	prep	with,interact
R2325	T3839	T3840	det	the,domain
R2326	T3840	T3838	pobj	domain,with
R2327	T3841	T3840	compound	PAX6,domain
R2328	T3842	T3840	compound	PST,domain
R2329	T3843	T3830	punct	", ",investigated
R233	T434	T432	pobj	anomalies,of
R2330	T3844	T3830	nsubj	we,investigated
R2331	T3845	T3830	advmod	next,investigated
R2332	T3846	T3847	advmod	how,affected
R2333	T3847	T3830	ccomp	affected,investigated
R2334	T3848	T3849	det	the,interactions
R2335	T3849	T3847	nsubjpass	interactions,affected
R2336	T3850	T3847	auxpass	were,affected
R2337	T3851	T3847	agent	by,affected
R2338	T3852	T3853	nummod	three,mutations
R2339	T3853	T3851	pobj	mutations,by
R234	T435	T434	amod	neural,anomalies
R2340	T3854	T3855	npadvmod	C,terminal
R2341	T3855	T3853	amod	terminal,mutations
R2342	T3856	T3855	punct	-,terminal
R2343	T3857	T3853	compound	PAX6,mutations
R2344	T3858	T3859	dep	that,described
R2345	T3859	T3853	relcl	described,mutations
R2346	T3860	T3859	aux	have,described
R2347	T3861	T3859	auxpass	been,described
R2348	T3862	T3859	advmod	previously,described
R2349	T3863	T3859	prep	in,described
R235	T436	T434	prep	in,anomalies
R2350	T3864	T3863	pobj	patients,in
R2351	T3865	T3864	prep	with,patients
R2352	T3866	T3867	amod	ocular,anomalies
R2353	T3867	T3865	pobj	anomalies,with
R2354	T3868	T3830	punct	.,investigated
R2355	T3870	T3871	det	The,mutation
R2356	T3871	T3873	nsubj	mutation,is
R2357	T3872	T3871	amod	first,mutation
R2358	T3874	T3875	det	a,substitution
R2359	T3875	T3873	attr	substitution,is
R236	T437	T436	pobj	patients,in
R2360	T3876	T3875	amod	single,substitution
R2361	T3877	T3875	compound	nucleotide,substitution
R2362	T3878	T3875	appos	1627A,substitution
R2363	T3879	T3880	punct	>,G
R2364	T3880	T3878	prep	G,1627A
R2365	T3881	T3882	dep	that,causes
R2366	T3882	T3875	relcl	causes,substitution
R2367	T3883	T3884	det	a,substitution
R2368	T3884	T3882	dobj	substitution,causes
R2369	T3885	T3884	nmod	glutamine,substitution
R237	T438	T437	prep	with,patients
R2370	T3886	T3885	prep	to,glutamine
R2371	T3887	T3886	pobj	arginine,to
R2372	T3888	T3889	compound	amino,acid
R2373	T3889	T3884	compound	acid,substitution
R2374	T3890	T3882	prep	in,causes
R2375	T3891	T3892	det	the,codon
R2376	T3892	T3890	pobj	codon,in
R2377	T3893	T3892	amod	last,codon
R2378	T3894	T3892	prep	of,codon
R2379	T3895	T3894	pobj	PAX6,of
R238	T439	T440	compound	PAX6,mutations
R2380	T3896	T3873	punct	.,is
R2381	T3898	T3899	det	This,mutation
R2382	T3899	T3901	nsubjpass	mutation,reported
R2383	T3900	T3899	amod	missense,mutation
R2384	T3902	T3899	punct	(,mutation
R2385	T3903	T3899	appos	Q422R,mutation
R2386	T3904	T3899	punct	),mutation
R2387	T3905	T3901	aux	has,reported
R2388	T3906	T3901	auxpass	been,reported
R2389	T3907	T3901	prep	in,reported
R239	T440	T438	pobj	mutations,with
R2390	T3908	T3909	nummod	two,patients
R2391	T3909	T3907	pobj	patients,in
R2392	T3910	T3909	punct	", ",patients
R2393	T3911	T3909	appos	one,patients
R2394	T3912	T3911	acl	affected,one
R2395	T3913	T3912	agent	by,affected
R2396	T3914	T3915	amod	anterior,dysgenesis
R2397	T3915	T3913	pobj	dysgenesis,by
R2398	T3916	T3915	compound	segment,dysgenesis
R2399	T3917	T3915	prep	with,dysgenesis
R24	T205	T206	det	a,regulator
R240	T441	T430	aux	could,explained
R2400	T3918	T3919	amod	uveal,ectropion
R2401	T3919	T3917	pobj	ectropion,with
R2402	T3920	T3911	cc	and,one
R2403	T3921	T3911	conj	one,one
R2404	T3922	T3921	prep	with,one
R2405	T3923	T3924	amod	typical,aniridia
R2406	T3924	T3922	pobj	aniridia,with
R2407	T3925	T3924	cc	and,aniridia
R2408	T3926	T3927	amod	foveal,hypoplasia
R2409	T3927	T3924	conj	hypoplasia,aniridia
R241	T442	T430	auxpass	be,explained
R2410	T3928	T3929	punct	[,33
R2411	T3929	T3901	parataxis	33,reported
R2412	T3930	T3929	nummod	27,33
R2413	T3931	T3929	punct	",",33
R2414	T3932	T3929	punct	],33
R2415	T3933	T3901	punct	.,reported
R2416	T3935	T3936	det	The,mutation
R2417	T3936	T3938	nsubjpass	mutation,found
R2418	T3937	T3936	amod	second,mutation
R2419	T3939	T3936	punct	(,mutation
R242	T443	T430	prep	by,explained
R2420	T3940	T3936	appos	1615del10,mutation
R2421	T3941	T3936	punct	),mutation
R2422	T3942	T3938	auxpass	was,found
R2423	T3943	T3938	prep	in,found
R2424	T3944	T3945	det	an,family
R2425	T3945	T3943	pobj	family,in
R2426	T3946	T3945	compound	aniridia,family
R2427	T3947	T3948	punct	[,28
R2428	T3948	T3938	parataxis	28,found
R2429	T3949	T3948	punct	],28
R243	T444	T445	amod	impaired,interactions
R2430	T3950	T3938	punct	.,found
R2431	T3952	T3953	det	This,deletion
R2432	T3953	T3957	nsubj	deletion,occurs
R2433	T3954	T3955	npadvmod	frame,shifting
R2434	T3955	T3953	amod	shifting,deletion
R2435	T3956	T3955	punct	-,shifting
R2436	T3958	T3959	advmod	just,before
R2437	T3959	T3957	prep	before,occurs
R2438	T3960	T3961	det	the,codon
R2439	T3961	T3959	pobj	codon,before
R244	T445	T443	pobj	interactions,by
R2440	T3962	T3961	compound	PAX6,codon
R2441	T3963	T3961	compound	stop,codon
R2442	T3964	T3957	cc	and,occurs
R2443	T3965	T3966	auxpass	is,predicted
R2444	T3966	T3957	conj	predicted,occurs
R2445	T3967	T3968	aux	to,cause
R2446	T3968	T3966	xcomp	cause,predicted
R2447	T3969	T3970	amod	translational,through
R2448	T3970	T3968	dobj	through,cause
R2449	T3971	T3970	compound	read,through
R245	T446	T447	nmod	protein,protein
R2450	T3972	T3970	punct	-,through
R2451	T3973	T3970	prep	into,through
R2452	T3974	T3975	det	the,region
R2453	T3975	T3973	pobj	region,into
R2454	T3976	T3975	nummod	3,region
R2455	T3977	T3976	punct	',3
R2456	T3978	T3975	amod	untranslated,region
R2457	T3979	T3968	punct	", ",cause
R2458	T3980	T3968	advcl	generating,cause
R2459	T3981	T3982	det	a,protein
R246	T447	T445	compound	protein,interactions
R2460	T3982	T3980	dobj	protein,generating
R2461	T3983	T3984	prep	in,replaced
R2462	T3984	T3982	relcl	replaced,protein
R2463	T3985	T3983	pobj	which,in
R2464	T3986	T3987	det	the,acids
R2465	T3987	T3984	nsubjpass	acids,replaced
R2466	T3988	T3987	amod	last,acids
R2467	T3989	T3987	nummod	5,acids
R2468	T3990	T3987	compound	amino,acids
R2469	T3991	T3987	prep	of,acids
R247	T448	T447	punct	-,protein
R2470	T3992	T3993	det	the,peptide
R2471	T3993	T3991	pobj	peptide,of
R2472	T3994	T3995	npadvmod	C,terminal
R2473	T3995	T3993	amod	terminal,peptide
R2474	T3996	T3995	punct	-,terminal
R2475	T3997	T3984	auxpass	are,replaced
R2476	T3998	T3984	agent	by,replaced
R2477	T3999	T4000	det	a,extension
R2478	T4000	T3998	pobj	extension,by
R2479	T4001	T4002	nummod	103,acid
R248	T449	T402	punct	.,propose
R2480	T4002	T4000	compound	acid,extension
R2481	T4003	T4002	compound	amino,acid
R2482	T4004	T4000	punct	-,extension
R2483	T4005	T3957	punct	.,occurs
R2484	T4007	T4008	amod	Affected,individuals
R2485	T4008	T4009	nsubj	individuals,showed
R2486	T4010	T4008	prep	in,individuals
R2487	T4011	T4012	det	this,family
R2488	T4012	T4010	pobj	family,in
R2489	T4013	T4014	amod	unusual,traits
R2490	T4014	T4009	dobj	traits,showed
R2491	T4015	T4014	amod	neurobehavioural,traits
R2492	T4016	T4014	prep	including,traits
R2493	T4017	T4018	amod	impaired,cognition
R2494	T4018	T4016	pobj	cognition,including
R2495	T4019	T4018	amod	social,cognition
R2496	T4020	T4018	cc	and,cognition
R2497	T4021	T4022	amod	poor,inhibition
R2498	T4022	T4018	conj	inhibition,cognition
R2499	T4023	T4022	amod	verbal,inhibition
R25	T206	T204	attr	regulator,is
R250	T175	T176	det	A,screen
R2500	T4024	T4025	punct	[,28
R2501	T4025	T4009	parataxis	28,showed
R2502	T4026	T4025	punct	],28
R2503	T4027	T4009	punct	.,showed
R2504	T4029	T4030	compound	MRI,analysis
R2505	T4030	T4031	nsubj	analysis,revealed
R2506	T4032	T4033	amod	grey,matter
R2507	T4033	T4034	compound	matter,abnormalities
R2508	T4034	T4031	dobj	abnormalities,revealed
R2509	T4035	T4034	prep	in,abnormalities
R2510	T4036	T4037	det	the,lobe
R2511	T4037	T4035	pobj	lobe,in
R2512	T4038	T4037	amod	frontal,lobe
R2513	T4039	T4037	punct	", ",lobe
R2514	T4040	T4041	amod	temporal,lobe
R2515	T4041	T4037	conj	lobe,lobe
R2516	T4042	T4041	cc	and,lobe
R2517	T4043	T4041	conj	cerebellum,lobe
R2518	T4044	T4034	punct	", ",abnormalities
R2519	T4045	T4034	cc	and,abnormalities
R2520	T4046	T4047	amod	white,matter
R2521	T4047	T4048	compound	matter,deficits
R2522	T4048	T4034	conj	deficits,abnormalities
R2523	T4049	T4048	prep	in,deficits
R2524	T4050	T4051	det	the,callosum
R2525	T4051	T4049	pobj	callosum,in
R2526	T4052	T4051	compound	corpus,callosum
R2527	T4053	T4054	punct	[,13
R2528	T4054	T4031	parataxis	13,revealed
R2529	T4055	T4054	punct	],13
R2530	T4056	T4031	punct	.,revealed
R2531	T4058	T4059	det	The,mutation
R2532	T4059	T4061	nsubjpass	mutation,reported
R2533	T4060	T4059	amod	third,mutation
R2534	T4062	T4059	appos	1629insT,mutation
R2535	T4063	T4062	punct	(,1629insT
R2536	T4064	T4062	appos	X423L,1629insT
R2537	T4065	T4059	punct	),mutation
R2538	T4066	T4061	aux	has,reported
R2539	T4067	T4061	auxpass	been,reported
R2540	T4068	T4061	prep	in,reported
R2541	T4069	T4070	amod	several,patients
R2542	T4070	T4068	pobj	patients,in
R2543	T4071	T4070	compound	aniridia,patients
R2544	T4072	T4073	punct	[,33
R2545	T4073	T4061	parataxis	33,reported
R2546	T4074	T4073	nummod	11,33
R2547	T4075	T4073	punct	",",33
R2548	T4076	T4073	nummod	12,33
R2549	T4077	T4073	punct	",",33
R2550	T4078	T4073	nummod	27,33
R2551	T4079	T4073	punct	",",33
R2552	T4080	T4073	punct	],33
R2553	T4081	T4061	punct	.,reported
R2554	T4083	T4084	nsubj	Insertion,changes
R2555	T4085	T4083	prep	of,Insertion
R2556	T4086	T4087	det	a,nucleotide
R2557	T4087	T4085	pobj	nucleotide,of
R2558	T4088	T4087	amod	single,nucleotide
R2559	T4089	T4087	compound	T,nucleotide
R2560	T4090	T4083	prep	at,Insertion
R2561	T4091	T4090	pobj	position,at
R2562	T4092	T4091	nummod	1629,position
R2563	T4093	T4094	det	the,codon
R2564	T4094	T4084	dobj	codon,changes
R2565	T4095	T4094	compound	stop,codon
R2566	T4096	T4094	punct	(,codon
R2567	T4097	T4094	appos	TAA,codon
R2568	T4098	T4094	punct	),codon
R2569	T4099	T4084	prep	to,changes
R257	T834	T835	det	The,protein
R2570	T4100	T4101	det	a,codon
R2571	T4101	T4099	pobj	codon,to
R2572	T4102	T4101	compound	leucine,codon
R2573	T4103	T4101	punct	(,codon
R2574	T4104	T4101	appos	TTA,codon
R2575	T4105	T4101	punct	),codon
R2576	T4106	T4084	cc	and,changes
R2577	T4107	T4084	conj	generates,changes
R2578	T4108	T4109	det	a,protein
R2579	T4109	T4107	dobj	protein,generates
R258	T835	T837	nsubj	protein,is
R2580	T4110	T4111	amod	full,length
R2581	T4111	T4109	compound	length,protein
R2582	T4112	T4109	compound	PAX6,protein
R2583	T4113	T4109	prep	with,protein
R2584	T4114	T4115	det	a,extension
R2585	T4115	T4113	pobj	extension,with
R2586	T4116	T4117	npadvmod	C,terminal
R2587	T4117	T4115	amod	terminal,extension
R2588	T4118	T4117	punct	-,terminal
R2589	T4119	T4120	dep	that,extends
R259	T836	T835	compound	PAX6,protein
R2590	T4120	T4115	relcl	extends,extension
R2591	T4121	T4120	prep	for,extends
R2592	T4122	T4123	det	a,acids
R2593	T4123	T4121	pobj	acids,for
R2594	T4124	T4123	amod	further,acids
R2595	T4125	T4123	nummod	35,acids
R2596	T4126	T4123	compound	amino,acids
R2597	T4127	T4120	prep	into,extends
R2598	T4128	T4129	det	the,region
R2599	T4129	T4127	pobj	region,into
R26	T207	T206	amod	transcriptional,regulator
R260	T838	T839	det	a,member
R2600	T4130	T4129	nummod	3,region
R2601	T4131	T4130	punct	',3
R2602	T4132	T4129	amod	untranslated,region
R2603	T4133	T4084	punct	.,changes
R2604	T4135	T4136	compound	MRI,analysis
R2605	T4136	T4137	nsubj	analysis,revealed
R2606	T4138	T4136	prep	of,analysis
R2607	T4139	T4140	nummod	six,patients
R2608	T4140	T4138	pobj	patients,of
R2609	T4141	T4140	prep	with,patients
R261	T839	T837	attr	member,is
R2610	T4142	T4143	det	this,mutation
R2611	T4143	T4141	pobj	mutation,with
R2612	T4144	T4145	amod	variable,defects
R2613	T4145	T4137	dobj	defects,revealed
R2614	T4146	T4145	compound	brain,defects
R2615	T4147	T4145	prep	including,defects
R2616	T4148	T4147	pobj	absence,including
R2617	T4149	T4148	cc	or,absence
R2618	T4150	T4148	conj	hypoplasia,absence
R2619	T4151	T4148	prep	of,absence
R262	T840	T839	prep	of,member
R2620	T4152	T4153	det	the,commissure
R2621	T4153	T4151	pobj	commissure,of
R2622	T4154	T4153	amod	anterior,commissure
R2623	T4155	T4153	punct	", ",commissure
R2624	T4156	T4157	amod	pineal,gland
R2625	T4157	T4153	conj	gland,commissure
R2626	T4158	T4157	cc	and,gland
R2627	T4159	T4160	amod	olfactory,bulbs
R2628	T4160	T4157	conj	bulbs,gland
R2629	T4161	T4162	punct	[,12
R263	T841	T842	det	the,family
R2630	T4162	T4137	parataxis	12,revealed
R2631	T4163	T4162	punct	],12
R2632	T4164	T4137	punct	.,revealed
R2633	T4166	T4167	nummod	Two,patients
R2634	T4167	T4168	nsubj	patients,had
R2635	T4168	T4169	ccomp	had,one
R2636	T4170	T4171	amod	temporal,polymicrogyria
R2637	T4171	T4168	dobj	polymicrogyria,had
R2638	T4172	T4169	punct	", ",one
R2639	T4173	T4169	prep	in,one
R264	T842	T840	pobj	family,of
R2640	T4174	T4173	pobj	association,in
R2641	T4175	T4174	prep	with,association
R2642	T4176	T4175	pobj	epilepsy,with
R2643	T4177	T4178	punct	[,12
R2644	T4178	T4169	parataxis	12,one
R2645	T4179	T4178	punct	],12
R2646	T4180	T4169	punct	.,one
R2647	T4182	T4183	det	The,mutations
R2648	T4183	T4185	nsubjpass	mutations,introduced
R2649	T4184	T4183	nummod	three,mutations
R265	T843	T842	nmod	PAX,family
R2650	T4186	T4185	auxpass	were,introduced
R2651	T4187	T4185	prep	into,introduced
R2652	T4188	T4189	det	the,construct
R2653	T4189	T4187	pobj	construct,into
R2654	T4190	T4189	compound	PAX6PST,construct
R2655	T4191	T4185	punct	", ",introduced
R2656	T4192	T4185	cc	and,introduced
R2657	T4193	T4194	amod	pairwise,tests
R2658	T4194	T4195	nsubjpass	tests,carried
R2659	T4195	T4185	conj	carried,introduced
R266	T844	T843	punct	(,PAX
R2660	T4196	T4195	auxpass	were,carried
R2661	T4197	T4195	prt	out,carried
R2662	T4198	T4199	aux	to,investigate
R2663	T4199	T4195	advcl	investigate,carried
R2664	T4200	T4201	det	the,interaction
R2665	T4201	T4199	dobj	interaction,investigate
R2666	T4202	T4201	prep	of,interaction
R2667	T4203	T4204	det	each,protein
R2668	T4204	T4202	pobj	protein,of
R2669	T4205	T4204	compound	mutant,protein
R267	T845	T846	amod	paired,box
R2670	T4206	T4201	prep	with,interaction
R2671	T4207	T4206	pobj	HOMER3,with
R2672	T4208	T4207	punct	", ",HOMER3
R2673	T4209	T4207	conj	DNCL1,HOMER3
R2674	T4210	T4209	cc	and,DNCL1
R2675	T4211	T4209	conj	TRIM11,DNCL1
R2676	T4212	T4195	punct	.,carried
R2677	T4214	T4215	det	All,mutations
R2678	T4215	T4217	nsubj	mutations,had
R2679	T4216	T4215	nummod	three,mutations
R268	T846	T843	appos	box,PAX
R2680	T4218	T4219	det	a,effect
R2681	T4219	T4217	dobj	effect,had
R2682	T4220	T4219	amod	clear,effect
R2683	T4221	T4219	prep	on,effect
R2684	T4222	T4223	det	the,interactions
R2685	T4223	T4221	pobj	interactions,on
R2686	T4224	T4217	punct	.,had
R2687	T4226	T4227	det	The,mutation
R2688	T4227	T4230	nsubj	mutation,caused
R2689	T4228	T4229	advmod	most,subtle
R269	T847	T846	punct	-,box
R2690	T4229	T4227	amod	subtle,mutation
R2691	T4231	T4227	punct	(,mutation
R2692	T4232	T4227	appos	Q422R,mutation
R2693	T4233	T4227	punct	),mutation
R2694	T4234	T4235	det	a,reduction
R2695	T4235	T4230	dobj	reduction,caused
R2696	T4236	T4235	prep	in,reduction
R2697	T4237	T4238	det	the,interaction
R2698	T4238	T4236	pobj	interaction,in
R2699	T4239	T4238	prep	with,interaction
R27	T208	T206	prep	with,regulator
R270	T848	T842	punct	),family
R2700	T4240	T4239	pobj	HOMER3,with
R2701	T4241	T4240	cc	and,HOMER3
R2702	T4242	T4240	conj	DNCL1,HOMER3
R2703	T4243	T4244	punct	(,Table
R2704	T4244	T4230	parataxis	Table,caused
R2705	T4245	T4244	dep	Figure,Table
R2706	T4246	T4245	nummod	3,Figure
R2707	T4247	T4244	punct	", ",Table
R2708	T4248	T4244	nummod	1,Table
R2709	T4249	T4244	punct	),Table
R271	T849	T842	prep	of,family
R2710	T4250	T4230	punct	.,caused
R2711	T4252	T4253	det	The,X423L
R2712	T4253	T4259	nsubj	X423L,reduced
R2713	T4254	T4255	npadvmod	C,terminal
R2714	T4255	T4253	amod	terminal,X423L
R2715	T4256	T4255	punct	-,terminal
R2716	T4257	T4253	compound	extension,X423L
R2717	T4258	T4253	compound	mutations,X423L
R2718	T4260	T4253	cc	and,X423L
R2719	T4261	T4262	compound	1615del10,mutations
R272	T850	T851	amod	transcriptional,regulators
R2720	T4262	T4253	conj	mutations,X423L
R2721	T4263	T4253	appos	both,X423L
R2722	T4264	T4259	advmod	dramatically,reduced
R2723	T4265	T4259	cc	or,reduced
R2724	T4266	T4259	conj	abolished,reduced
R2725	T4267	T4268	det	the,interaction
R2726	T4268	T4266	dobj	interaction,abolished
R2727	T4269	T4268	prep	with,interaction
R2728	T4270	T4269	pobj	HOMER3,with
R2729	T4271	T4270	cc	and,HOMER3
R273	T851	T849	pobj	regulators,of
R2730	T4272	T4270	conj	DNCL1,HOMER3
R2731	T4273	T4274	punct	(,Table
R2732	T4274	T4266	parataxis	Table,abolished
R2733	T4275	T4274	dep	Figure,Table
R2734	T4276	T4275	nummod	3,Figure
R2735	T4277	T4274	punct	", ",Table
R2736	T4278	T4274	nummod	1,Table
R2737	T4279	T4274	punct	),Table
R2738	T4280	T4259	punct	.,reduced
R2739	T4282	T4283	nsubj	None,affected
R274	T852	T837	cc	and,is
R2740	T4284	T4282	prep	of,None
R2741	T4285	T4286	det	the,mutations
R2742	T4286	T4284	pobj	mutations,of
R2743	T4287	T4286	nummod	three,mutations
R2744	T4288	T4289	det	the,interaction
R2745	T4289	T4283	dobj	interaction,affected
R2746	T4290	T4289	prep	with,interaction
R2747	T4291	T4290	pobj	TRIM11,with
R2748	T4292	T4293	dep	which,is
R2749	T4293	T4283	advcl	is,affected
R275	T853	T837	conj	is,is
R2750	T4294	T4293	advmod	again,is
R2751	T4295	T4293	acomp	consistent,is
R2752	T4296	T4295	prep	with,consistent
R2753	T4297	T4298	det	the,hypothesis
R2754	T4298	T4296	pobj	hypothesis,with
R2755	T4299	T4300	mark	that,interacts
R2756	T4300	T4298	acl	interacts,hypothesis
R2757	T4301	T4300	nsubj	TRIM11,interacts
R2758	T4302	T4300	prep	with,interacts
R2759	T4303	T4304	det	a,part
R276	T854	T853	acomp	essential,is
R2760	T4304	T4302	pobj	part,with
R2761	T4305	T4306	advmod	more,terminal
R2762	T4306	T4304	amod	terminal,part
R2763	T4307	T4306	npadvmod	N,terminal
R2764	T4308	T4306	punct	-,terminal
R2765	T4309	T4304	prep	of,part
R2766	T4310	T4311	det	the,domain
R2767	T4311	T4309	pobj	domain,of
R2768	T4312	T4311	compound	PST,domain
R2769	T4313	T4283	punct	.,affected
R277	T855	T854	prep	for,essential
R2778	T5078	T5079	prep	On,defined
R2779	T5080	T5081	det	the,basis
R278	T856	T857	amod	normal,development
R2780	T5081	T5078	pobj	basis,On
R2781	T5082	T5081	prep	of,basis
R2782	T5083	T5084	amod	secondary,structure
R2783	T5084	T5085	compound	structure,predictions
R2784	T5085	T5082	pobj	predictions,of
R2785	T5086	T5085	cc	and,predictions
R2786	T5087	T5088	compound	amino,acid
R2787	T5088	T5089	compound	acid,conservation
R2788	T5089	T5085	conj	conservation,predictions
R2789	T5090	T5089	compound	sequence,conservation
R279	T857	T855	pobj	development,for
R2790	T5091	T5079	punct	", ",defined
R2791	T5092	T5079	nsubj	we,defined
R2792	T5093	T5094	det	a,domain
R2793	T5094	T5079	dobj	domain,defined
R2794	T5095	T5094	amod	novel,domain
R2795	T5096	T5094	compound	PAX6,domain
R2796	T5097	T5094	compound	protein,domain
R2797	T5098	T5094	punct	", ",domain
R2798	T5099	T5100	dep	which,called
R2799	T5100	T5094	relcl	called,domain
R28	T209	T210	det	a,role
R280	T858	T857	amod	ocular,development
R2800	T5101	T5100	nsubj	we,called
R2801	T5102	T5100	aux	have,called
R2802	T5103	T5104	det	the,peptide
R2803	T5104	T5100	oprd	peptide,called
R2804	T5105	T5106	npadvmod	C,terminal
R2805	T5106	T5104	amod	terminal,peptide
R2806	T5107	T5106	punct	-,terminal
R2807	T5108	T5079	punct	.,defined
R2808	T5110	T5111	nsubj	We,performed
R2809	T5112	T5113	nmod	yeast,screens
R281	T859	T858	cc	and,ocular
R2810	T5113	T5111	dobj	screens,performed
R2811	T5114	T5115	nummod	two,hybrid
R2812	T5115	T5113	compound	hybrid,screens
R2813	T5116	T5115	punct	-,hybrid
R2814	T5117	T5113	compound	library,screens
R2815	T5118	T5111	prep	with,performed
R2816	T5119	T5120	det	the,peptide
R2817	T5120	T5118	pobj	peptide,with
R2818	T5121	T5122	npadvmod	C,terminal
R2819	T5122	T5120	amod	terminal,peptide
R282	T860	T858	conj	neural,ocular
R2820	T5123	T5122	punct	-,terminal
R2821	T5124	T5120	cc	and,peptide
R2822	T5125	T5126	det	the,domain
R2823	T5126	T5120	conj	domain,peptide
R2824	T5127	T5126	amod	whole,domain
R2825	T5128	T5126	compound	PST,domain
R2826	T5129	T5111	cc	and,performed
R2827	T5130	T5131	nsubj	we,identified
R2828	T5131	T5111	conj	identified,performed
R2829	T5132	T5133	nummod	three,proteins
R283	T861	T862	punct	[,1
R2830	T5133	T5131	dobj	proteins,identified
R2831	T5134	T5133	amod	novel,proteins
R2832	T5135	T5133	amod	interacting,proteins
R2833	T5136	T5133	punct	", ",proteins
R2834	T5137	T5133	appos	HOMER3,proteins
R2835	T5138	T5137	punct	", ",HOMER3
R2836	T5139	T5137	conj	DNCL1,HOMER3
R2837	T5140	T5139	cc	and,DNCL1
R2838	T5141	T5139	conj	TRIM11,DNCL1
R2839	T5142	T5131	punct	.,identified
R284	T862	T853	parataxis	1,is
R2840	T5144	T5145	prep	In,interacted
R2841	T5146	T5147	compound	library,screens
R2842	T5147	T5144	pobj	screens,In
R2843	T5148	T5145	punct	", ",interacted
R2844	T5149	T5145	nsubj	HOMER3,interacted
R2845	T5150	T5149	cc	and,HOMER3
R2846	T5151	T5149	conj	DNCL1,HOMER3
R2847	T5152	T5145	prep	with,interacted
R2848	T5153	T5154	det	the,peptide
R2849	T5154	T5152	pobj	peptide,with
R285	T863	T862	punct	],1
R2850	T5155	T5156	npadvmod	C,terminal
R2851	T5156	T5154	amod	terminal,peptide
R2852	T5157	T5156	punct	-,terminal
R2853	T5158	T5154	cc	and,peptide
R2854	T5159	T5160	det	the,domain
R2855	T5160	T5154	conj	domain,peptide
R2856	T5161	T5160	compound	PST,domain
R2857	T5162	T5163	mark	while,interacted
R2858	T5163	T5145	advcl	interacted,interacted
R2859	T5164	T5163	nsubj	TRIM11,interacted
R286	T864	T837	punct	.,is
R2860	T5165	T5166	advmod	only,with
R2861	T5166	T5163	prep	with,interacted
R2862	T5167	T5168	det	the,domain
R2863	T5168	T5166	pobj	domain,with
R2864	T5169	T5168	compound	PST,domain
R2865	T5170	T5145	punct	", ",interacted
R2866	T5171	T5145	advcl	suggesting,interacted
R2867	T5172	T5173	mark	that,interact
R2868	T5173	T5171	ccomp	interact,suggesting
R2869	T5174	T5173	nsubj	HOMER3,interact
R287	T866	T867	amod	Heterozygous,mutations
R2870	T5175	T5174	cc	and,HOMER3
R2871	T5176	T5174	conj	DNCL1,HOMER3
R2872	T5177	T5173	advmod	specifically,interact
R2873	T5178	T5173	prep	with,interact
R2874	T5179	T5180	det	the,peptide
R2875	T5180	T5178	pobj	peptide,with
R2876	T5181	T5182	npadvmod	C,terminal
R2877	T5182	T5180	amod	terminal,peptide
R2878	T5183	T5182	punct	-,terminal
R2879	T5184	T5185	mark	while,interacts
R288	T867	T868	nsubj	mutations,cause
R2880	T5185	T5173	advcl	interacts,interact
R2881	T5186	T5185	nsubj	TRIM11,interacts
R2882	T5187	T5185	prep	with,interacts
R2883	T5188	T5189	det	a,part
R2884	T5189	T5187	pobj	part,with
R2885	T5190	T5191	advmod	more,terminal
R2886	T5191	T5189	amod	terminal,part
R2887	T5192	T5191	npadvmod	N,terminal
R2888	T5193	T5191	punct	-,terminal
R2889	T5194	T5189	prep	of,part
R289	T869	T867	prep	of,mutations
R2890	T5195	T5196	det	the,domain
R2891	T5196	T5194	pobj	domain,of
R2892	T5197	T5196	compound	PST,domain
R2893	T5198	T5145	punct	.,interacted
R2894	T5200	T5201	det	The,interactions
R2895	T5201	T5202	nsubjpass	interactions,confirmed
R2896	T5203	T5201	prep	between,interactions
R2897	T5204	T5205	det	the,domain
R2898	T5205	T5203	pobj	domain,between
R2899	T5206	T5205	compound	PST,domain
R29	T210	T208	pobj	role,with
R290	T870	T871	det	the,gene
R2900	T5207	T5205	cc	and,domain
R2901	T5208	T5205	conj	HOMER3,domain
R2902	T5209	T5208	punct	", ",HOMER3
R2903	T5210	T5208	conj	DNCL1,HOMER3
R2904	T5211	T5210	cc	and,DNCL1
R2905	T5212	T5210	conj	TRIM11,DNCL1
R2906	T5213	T5202	auxpass	were,confirmed
R2907	T5214	T5202	prep	in,confirmed
R2908	T5215	T5216	amod	pairwise,tests
R2909	T5216	T5214	pobj	tests,in
R291	T871	T869	pobj	gene,of
R2910	T5217	T5202	punct	.,confirmed
R2911	T5219	T5220	nsubj	We,were
R2912	T5221	T5220	neg	not,were
R2913	T5222	T5220	acomp	able,were
R2914	T5223	T5224	aux	to,confirm
R2915	T5224	T5222	xcomp	confirm,able
R2916	T5225	T5226	det	the,interaction
R2917	T5226	T5224	dobj	interaction,confirm
R2918	T5227	T5226	prep	between,interaction
R2919	T5228	T5227	pobj	HOMER3,between
R292	T872	T871	amod	human,gene
R2920	T5229	T5228	cc	or,HOMER3
R2921	T5230	T5228	conj	DNCL1,HOMER3
R2922	T5231	T5224	prep	with,confirm
R2923	T5232	T5233	det	the,construct
R2924	T5233	T5231	pobj	construct,with
R2925	T5234	T5235	npadvmod	C,terminal
R2926	T5235	T5233	amod	terminal,construct
R2927	T5236	T5235	punct	-,terminal
R2928	T5237	T5233	compound	peptide,construct
R2929	T5238	T5224	prep	in,confirm
R293	T873	T871	compound	PAX6,gene
R2930	T5239	T5240	amod	pairwise,tests
R2931	T5240	T5238	pobj	tests,in
R2932	T5241	T5220	punct	", ",were
R2933	T5242	T5220	cc	but,were
R2934	T5243	T5244	nsubj	we,showed
R2935	T5244	T5220	conj	showed,were
R2936	T5245	T5246	mark	that,was
R2937	T5246	T5244	ccomp	was,showed
R2938	T5247	T5248	det	the,peptide
R2939	T5248	T5246	nsubj	peptide,was
R294	T874	T868	dobj	aniridia,cause
R2940	T5249	T5250	npadvmod	C,terminal
R2941	T5250	T5248	amod	terminal,peptide
R2942	T5251	T5250	punct	-,terminal
R2943	T5252	T5246	acomp	important,was
R2944	T5253	T5252	prep	for,important
R2945	T5254	T5255	nmod	PAX6,HOMER3
R2946	T5255	T5257	nmod	HOMER3,interaction
R2947	T5256	T5255	punct	/,HOMER3
R2948	T5257	T5253	pobj	interaction,for
R2949	T5258	T5255	cc	or,HOMER3
R295	T875	T874	punct	(,aniridia
R2950	T5259	T5260	compound	PAX6,DNCL1
R2951	T5260	T5255	conj	DNCL1,HOMER3
R2952	T5261	T5260	punct	/,DNCL1
R2953	T5262	T5263	mark	because,interact
R2954	T5263	T5246	advcl	interact,was
R2955	T5264	T5263	nsubj	HOMER3,interact
R2956	T5265	T5264	cc	and,HOMER3
R2957	T5266	T5264	conj	DNCL1,HOMER3
R2958	T5267	T5263	aux	did,interact
R2959	T5268	T5263	neg	not,interact
R296	T876	T874	appos	absence,aniridia
R2960	T5269	T5263	prep	with,interact
R2961	T5270	T5271	det	a,construct
R2962	T5271	T5269	pobj	construct,with
R2963	T5272	T5271	compound	PST,construct
R2964	T5273	T5271	compound	domain,construct
R2965	T5274	T5271	acl	lacking,construct
R2966	T5275	T5276	det	the,peptide
R2967	T5276	T5274	dobj	peptide,lacking
R2968	T5277	T5278	npadvmod	C,terminal
R2969	T5278	T5276	amod	terminal,peptide
R297	T877	T876	prep	of,absence
R2970	T5279	T5278	punct	-,terminal
R2971	T5280	T5244	punct	.,showed
R2972	T5282	T5283	nsubjpass	HOMER3,found
R2973	T5284	T5283	auxpass	is,found
R2974	T5285	T5283	prep	in,found
R2975	T5286	T5287	det	the,PSD
R2976	T5287	T5285	pobj	PSD,in
R2977	T5288	T5287	prep	of,PSD
R2978	T5289	T5288	pobj	neurons,of
R2979	T5290	T5283	cc	and,found
R298	T878	T879	det	the,iris
R2980	T5291	T5292	advmod	directly,binds
R2981	T5292	T5283	conj	binds,found
R2982	T5293	T5292	prep	to,binds
R2983	T5294	T5295	nmod	type,receptors
R2984	T5295	T5293	pobj	receptors,to
R2985	T5296	T5294	nummod	I,type
R2986	T5297	T5298	amod	metabotropic,glutamate
R2987	T5298	T5295	compound	glutamate,receptors
R2988	T5299	T5295	punct	", ",receptors
R2989	T5300	T5301	dep	which,act
R299	T879	T877	pobj	iris,of
R2990	T5301	T5295	relcl	act,receptors
R2991	T5302	T5301	prep	via,act
R2992	T5303	T5304	compound	phospholipase,C
R2993	T5304	T5302	pobj	C,via
R2994	T5305	T5306	aux	to,stimulate
R2995	T5306	T5301	advcl	stimulate,act
R2996	T5307	T5308	npadvmod	IP3,mediated
R2997	T5308	T5310	amod	mediated,release
R2998	T5309	T5308	punct	-,mediated
R2999	T5310	T5306	dobj	release,stimulate
R3	T179	T176	prep	for,screen
R30	T211	T210	amod	key,role
R300	T880	T874	punct	),aniridia
R3000	T5311	T5310	prep	of,release
R3001	T5312	T5311	pobj	Ca2+,of
R3002	T5313	T5310	prep	from,release
R3003	T5314	T5315	amod	intracellular,vesicles
R3004	T5315	T5313	pobj	vesicles,from
R3005	T5316	T5317	punct	[,39
R3006	T5317	T5292	parataxis	39,binds
R3007	T5318	T5317	nummod	35,39
R3008	T5319	T5317	punct	",",39
R3009	T5320	T5317	punct	],39
R301	T881	T874	cc	and,aniridia
R3010	T5321	T5283	punct	.,found
R3011	T5323	T5324	nsubj	HOMER3,is
R3012	T5325	T5326	det	a,member
R3013	T5326	T5324	attr	member,is
R3014	T5327	T5326	prep	of,member
R3015	T5328	T5329	det	the,family
R3016	T5329	T5327	pobj	family,of
R3017	T5330	T5329	compound	HOMER,family
R3018	T5331	T5329	prep	of,family
R3019	T5332	T5331	pobj	proteins,of
R302	T882	T883	det	a,range
R3020	T5333	T5334	dep	that,expressed
R3021	T5334	T5329	relcl	expressed,family
R3022	T5335	T5334	auxpass	are,expressed
R3023	T5336	T5334	advmod	constitutively,expressed
R3024	T5337	T5334	prep	in,expressed
R3025	T5338	T5339	det	the,brain
R3026	T5339	T5337	pobj	brain,in
R3027	T5340	T5334	cc	and,expressed
R3028	T5341	T5334	conj	play,expressed
R3029	T5342	T5343	det	a,role
R303	T883	T874	conj	range,aniridia
R3030	T5343	T5341	dobj	role,play
R3031	T5344	T5341	prep	in,play
R3032	T5345	T5346	amod	post-synaptic,signalling
R3033	T5346	T5344	pobj	signalling,in
R3034	T5347	T5346	cc	and,signalling
R3035	T5348	T5349	compound	receptor,trafficking
R3036	T5349	T5346	conj	trafficking,signalling
R3037	T5350	T5341	prep	by,play
R3038	T5351	T5350	pcomp	forming,by
R3039	T5352	T5353	amod	multivalent,links
R304	T884	T883	prep	of,range
R3040	T5353	T5351	dobj	links,forming
R3041	T5354	T5353	prep	with,links
R3042	T5355	T5356	amod	various,receptors
R3043	T5356	T5354	pobj	receptors,with
R3044	T5357	T5356	cc	and,receptors
R3045	T5358	T5359	compound	PSD,proteins
R3046	T5359	T5356	conj	proteins,receptors
R3047	T5360	T5359	compound	scaffolding,proteins
R3048	T5361	T5362	punct	[,35
R3049	T5362	T5324	parataxis	35,is
R305	T885	T886	amod	other,malformations
R3050	T5363	T5362	punct	",",35
R3051	T5364	T5362	appos	39,35
R3052	T5365	T5366	punct	-,41
R3053	T5366	T5364	prep	41,39
R3054	T5367	T5362	punct	],35
R3055	T5368	T5324	punct	.,is
R3056	T5370	T5371	compound	HOMER,proteins
R3057	T5371	T5372	nsubjpass	proteins,implicated
R3058	T5373	T5372	aux	have,implicated
R3059	T5374	T5372	advmod	also,implicated
R306	T886	T884	pobj	malformations,of
R3060	T5375	T5372	auxpass	been,implicated
R3061	T5376	T5372	prep	in,implicated
R3062	T5377	T5378	compound	axon,guidance
R3063	T5378	T5376	pobj	guidance,in
R3064	T5379	T5372	prep	during,implicated
R3065	T5380	T5381	compound	brain,development
R3066	T5381	T5379	pobj	development,during
R3067	T5382	T5383	punct	[,42
R3068	T5383	T5372	parataxis	42,implicated
R3069	T5384	T5383	punct	],42
R307	T887	T886	amod	congenital,malformations
R3070	T5385	T5372	punct	.,implicated
R3071	T5387	T5388	nsubj	DNCL1,is
R3072	T5389	T5390	det	a,subunit
R3073	T5390	T5388	attr	subunit,is
R3074	T5391	T5390	prep	of,subunit
R3075	T5392	T5393	nummod	two,complexes
R3076	T5393	T5391	pobj	complexes,of
R3077	T5394	T5395	amod	intracellular,transport
R3078	T5395	T5393	compound	transport,complexes
R3079	T5396	T5393	compound	protein,complexes
R308	T888	T886	compound	eye,malformations
R3080	T5397	T5393	punct	", ",complexes
R3081	T5398	T5393	appos	dynein,complexes
R3082	T5399	T5398	cc	and,dynein
R3083	T5400	T5401	compound	myosin,Va
R3084	T5401	T5398	conj	Va,dynein
R3085	T5402	T5403	punct	[,36
R3086	T5403	T5388	parataxis	36,is
R3087	T5404	T5403	punct	],36
R3088	T5405	T5388	punct	.,is
R3089	T5407	T5408	nsubjpass	Dynein,involved
R309	T889	T890	punct	[,2
R3090	T5409	T5407	cc	and,Dynein
R3091	T5410	T5411	compound	myosin,Va
R3092	T5411	T5407	conj	Va,Dynein
R3093	T5412	T5408	auxpass	are,involved
R3094	T5413	T5408	prep	in,involved
R3095	T5414	T5415	det	the,movement
R3096	T5415	T5413	pobj	movement,in
R3097	T5416	T5417	npadvmod	microtubule,based
R3098	T5417	T5415	amod	based,movement
R3099	T5418	T5417	punct	-,based
R31	T212	T210	prep	in,role
R310	T890	T868	parataxis	2,cause
R3100	T5419	T5417	cc	and,based
R3101	T5420	T5421	npadvmod	actin,based
R3102	T5421	T5417	conj	based,based
R3103	T5422	T5421	punct	-,based
R3104	T5423	T5408	advmod	respectively,involved
R3105	T5424	T5408	prep	of,involved
R3106	T5425	T5424	pobj	proteins,of
R3107	T5426	T5425	punct	", ",proteins
R3108	T5427	T5425	conj	organelles,proteins
R3109	T5428	T5427	cc	and,organelles
R311	T891	T890	punct	],2
R3110	T5429	T5427	conj	vesicles,organelles
R3111	T5430	T5425	prep	in,proteins
R3112	T5431	T5430	pobj	neurons,in
R3113	T5432	T5433	punct	[,37
R3114	T5433	T5408	parataxis	37,involved
R3115	T5434	T5433	nummod	36,37
R3116	T5435	T5433	punct	",",37
R3117	T5436	T5433	punct	],37
R3118	T5437	T5408	punct	.,involved
R3119	T5439	T5440	compound	Myosin,Va
R312	T892	T868	punct	.,cause
R3120	T5440	T5441	nsubjpass	Va,enriched
R3121	T5442	T5441	auxpass	is,enriched
R3122	T5443	T5441	prep	in,enriched
R3123	T5444	T5445	det	the,PSD
R3124	T5445	T5443	pobj	PSD,in
R3125	T5446	T5447	punct	[,43
R3126	T5447	T5441	parataxis	43,enriched
R3127	T5448	T5447	punct	],43
R3128	T5449	T5441	punct	", ",enriched
R3129	T5450	T5441	cc	and,enriched
R313	T894	T895	amod	Neural,defects
R3130	T5451	T5452	nsubj	DNCL1,binds
R3131	T5452	T5441	conj	binds,enriched
R3132	T5453	T5452	prep	to,binds
R3133	T5454	T5455	det	a,variety
R3134	T5455	T5453	pobj	variety,to
R3135	T5456	T5455	prep	of,variety
R3136	T5457	T5458	compound	PSD,proteins
R3137	T5458	T5456	pobj	proteins,of
R3138	T5459	T5458	prep	including,proteins
R3139	T5460	T5461	nmod	guanylate,protein
R314	T895	T896	nsubj	defects,are
R3140	T5461	T5459	pobj	protein,including
R3141	T5462	T5461	nmod	kinase,protein
R3142	T5463	T5464	npadvmod	domain,associated
R3143	T5464	T5461	amod	associated,protein
R3144	T5465	T5464	punct	-,associated
R3145	T5466	T5467	punct	[,44
R3146	T5467	T5461	parataxis	44,protein
R3147	T5468	T5467	punct	],44
R3148	T5469	T5461	cc	and,protein
R3149	T5470	T5471	amod	neuronal,synthase
R315	T897	T898	amod	such,as
R3150	T5471	T5461	conj	synthase,protein
R3151	T5472	T5473	amod	nitric,oxide
R3152	T5473	T5471	compound	oxide,synthase
R3153	T5474	T5475	punct	[,45
R3154	T5475	T5471	parataxis	45,synthase
R3155	T5476	T5475	punct	],45
R3156	T5477	T5452	punct	.,binds
R3157	T5479	T5480	nsubj	TRIM11,is
R3158	T5481	T5482	det	a,member
R3159	T5482	T5480	attr	member,is
R316	T898	T895	prep	as,defects
R3160	T5483	T5482	prep	of,member
R3161	T5484	T5485	det	the,family
R3162	T5485	T5483	pobj	family,of
R3163	T5486	T5485	nmod	mouse,family
R3164	T5487	T5488	amod	tripartite,motif
R3165	T5488	T5485	compound	motif,family
R3166	T5489	T5485	compound	protein,family
R3167	T5490	T5485	punct	(,family
R3168	T5491	T5492	advmod	also,known
R3169	T5492	T5485	acl	known,family
R317	T899	T900	amod	foveal,hypoplasia
R3170	T5493	T5492	prep	as,known
R3171	T5494	T5495	det	the,family
R3172	T5495	T5493	pobj	family,as
R3173	T5496	T5495	compound	RBCC,family
R3174	T5497	T5480	punct	),is
R3175	T5498	T5480	punct	", ",is
R3176	T5499	T5480	cc	and,is
R3177	T5500	T5480	conj	contains,is
R3178	T5501	T5502	det	the,motifs
R3179	T5502	T5500	dobj	motifs,contains
R318	T900	T898	pobj	hypoplasia,as
R3180	T5503	T5502	nummod	three,motifs
R3181	T5504	T5502	amod	characteristic,motifs
R3182	T5505	T5502	amod	structural,motifs
R3183	T5506	T5502	prep	of,motifs
R3184	T5507	T5508	det	this,family
R3185	T5508	T5506	pobj	family,of
R3186	T5509	T5508	compound	protein,family
R3187	T5510	T5502	punct	", ",motifs
R3188	T5511	T5512	det	a,finger
R3189	T5512	T5502	appos	finger,motifs
R319	T901	T900	cc	and,hypoplasia
R3190	T5513	T5512	compound	RING,finger
R3191	T5514	T5512	punct	", ",finger
R3192	T5515	T5516	det	a,finger
R3193	T5516	T5512	conj	finger,finger
R3194	T5517	T5518	compound	B,box
R3195	T5518	T5516	compound	box,finger
R3196	T5519	T5518	punct	-,box
R3197	T5520	T5516	compound	zinc,finger
R3198	T5521	T5516	punct	", ",finger
R3199	T5522	T5516	cc	and,finger
R32	T213	T214	amod	ocular,development
R320	T902	T903	amod	optic,nerve
R3200	T5523	T5524	det	a,domain
R3201	T5524	T5516	conj	domain,finger
R3202	T5525	T5526	amod	coiled,coil
R3203	T5526	T5524	compound	coil,domain
R3204	T5527	T5502	punct	", ",motifs
R3205	T5528	T5529	advmod	as,as
R3206	T5529	T5502	cc	as,motifs
R3207	T5530	T5529	advmod	well,as
R3208	T5531	T5532	det	a,domain
R3209	T5532	T5502	conj	domain,motifs
R321	T903	T904	compound	nerve,hypoplasia
R3210	T5533	T5532	compound	B30.2,domain
R3211	T5534	T5535	dep	that,found
R3212	T5535	T5532	relcl	found,domain
R3213	T5536	T5535	auxpass	is,found
R3214	T5537	T5535	prep	in,found
R3215	T5538	T5537	pobj	many,in
R3216	T5539	T5538	cc	but,many
R3217	T5540	T5539	neg	not,but
R3218	T5541	T5542	nmod	all,proteins
R3219	T5542	T5538	conj	proteins,many
R322	T904	T900	conj	hypoplasia,hypoplasia
R3220	T5543	T5542	compound	TRIM,proteins
R3221	T5544	T5545	punct	[,38
R3222	T5545	T5500	parataxis	38,contains
R3223	T5546	T5545	punct	],38
R3224	T5547	T5480	punct	.,is
R3225	T5549	T5550	nsubj	TRIM11,interacts
R3226	T5551	T5550	prep	with,interacts
R3227	T5552	T5551	pobj	Humanin,with
R3228	T5553	T5552	punct	", ",Humanin
R3229	T5554	T5555	det	a,protein
R323	T905	T896	acomp	common,are
R3230	T5555	T5552	appos	protein,Humanin
R3231	T5556	T5557	dep	that,suppresses
R3232	T5557	T5555	relcl	suppresses,protein
R3233	T5558	T5559	det	the,neurotoxicity
R3234	T5559	T5557	dobj	neurotoxicity,suppresses
R3235	T5560	T5559	acl	associated,neurotoxicity
R3236	T5561	T5560	prep	with,associated
R3237	T5562	T5563	poss	Alzheimer,disease
R3238	T5563	T5561	pobj	disease,with
R3239	T5564	T5562	case	's,Alzheimer
R324	T906	T896	prep	in,are
R3240	T5565	T5566	punct	[,46
R3241	T5566	T5550	parataxis	46,interacts
R3242	T5567	T5566	punct	],46
R3243	T5568	T5550	punct	.,interacts
R3244	T5570	T5571	nsubj	TRIM11,lowers
R3245	T5572	T5573	compound	Humanin,levels
R3246	T5573	T5571	dobj	levels,lowers
R3247	T5574	T5571	prep	by,lowers
R3248	T5575	T5576	det	a,mechanism
R3249	T5576	T5574	pobj	mechanism,by
R325	T907	T908	npadvmod	PAX6,associated
R3250	T5577	T5578	dep	that,appears
R3251	T5578	T5576	relcl	appears,mechanism
R3252	T5579	T5580	aux	to,involve
R3253	T5580	T5578	xcomp	involve,appears
R3254	T5581	T5582	npadvmod	ubiqutin,mediated
R3255	T5582	T5584	amod	mediated,degradation
R3256	T5583	T5582	punct	-,mediated
R3257	T5584	T5580	dobj	degradation,involve
R3258	T5585	T5584	amod	proteasomal,degradation
R3259	T5586	T5587	punct	[,46
R326	T908	T910	amod	associated,disease
R3260	T5587	T5571	parataxis	46,lowers
R3261	T5588	T5587	punct	],46
R3262	T5589	T5571	punct	.,lowers
R3263	T5591	T5592	prep	At,considered
R3264	T5593	T5591	amod	present,At
R3265	T5594	T5595	poss	our,data
R3266	T5595	T5592	nsubjpass	data,considered
R3267	T5596	T5592	aux	must,considered
R3268	T5597	T5592	auxpass	be,considered
R3269	T5598	T5592	oprd	preliminary,considered
R327	T909	T908	punct	-,associated
R3270	T5599	T5600	mark	because,confirmed
R3271	T5600	T5592	advcl	confirmed,considered
R3272	T5601	T5602	det	the,interactions
R3273	T5602	T5600	nsubjpass	interactions,confirmed
R3274	T5603	T5600	aux	have,confirmed
R3275	T5604	T5600	neg	not,confirmed
R3276	T5605	T5600	auxpass	been,confirmed
R3277	T5606	T5600	prep	by,confirmed
R3278	T5607	T5608	det	any,approach
R3279	T5608	T5606	pobj	approach,by
R328	T910	T906	pobj	disease,in
R3280	T5609	T5608	amod	other,approach
R3281	T5610	T5592	punct	.,considered
R3282	T5612	T5613	advmod	However,is
R3283	T5614	T5613	nsubj	it,is
R3284	T5615	T5613	acomp	interesting,is
R3285	T5616	T5617	aux	to,speculate
R3286	T5617	T5613	xcomp	speculate,is
R3287	T5618	T5619	mark	that,be
R3288	T5619	T5617	ccomp	be,speculate
R3289	T5620	T5621	det	the,interaction
R329	T911	T910	compound	eye,disease
R3290	T5621	T5619	nsubj	interaction,be
R3291	T5622	T5621	prep	of,interaction
R3292	T5623	T5622	pobj	PAX6,of
R3293	T5624	T5621	prep	with,interaction
R3294	T5625	T5624	pobj	HOMER3,with
R3295	T5626	T5625	cc	and,HOMER3
R3296	T5627	T5625	conj	DNCL1,HOMER3
R3297	T5628	T5619	aux	may,be
R3298	T5629	T5630	det	the,basis
R3299	T5630	T5619	attr	basis,be
R33	T214	T212	pobj	development,in
R330	T912	T913	punct	[,3
R3300	T5631	T5630	prep	of,basis
R3301	T5632	T5633	det	a,mechanism
R3302	T5633	T5631	pobj	mechanism,of
R3303	T5634	T5635	prep	by,causes
R3304	T5635	T5633	relcl	causes,mechanism
R3305	T5636	T5634	pobj	which,by
R3306	T5637	T5638	amod	synaptic,signalling
R3307	T5638	T5635	nsubj	signalling,causes
R3308	T5639	T5635	dobj	changes,causes
R3309	T5640	T5639	prep	in,changes
R331	T913	T896	parataxis	3,are
R3310	T5641	T5642	compound	gene,expression
R3311	T5642	T5640	pobj	expression,in
R3312	T5643	T5613	punct	.,is
R3313	T5645	T5646	nsubj	We,propose
R3314	T5647	T5648	mark	that,sequestered
R3315	T5648	T5646	ccomp	sequestered,propose
R3316	T5649	T5648	nsubjpass	PAX6,sequestered
R3317	T5650	T5648	auxpass	is,sequestered
R3318	T5651	T5648	prep	in,sequestered
R3319	T5652	T5653	det	the,PSD
R332	T914	T915	punct	-,5
R3320	T5653	T5651	pobj	PSD,in
R3321	T5654	T5648	prep	by,sequestered
R3322	T5655	T5654	pcomp	binding,by
R3323	T5656	T5655	prep	to,binding
R3324	T5657	T5656	pobj	HOMER3,to
R3325	T5658	T5646	punct	.,propose
R3326	T5660	T5661	mark	Since,causes
R3327	T5661	T5664	advcl	causes,released
R3328	T5662	T5663	compound	receptor,activation
R3329	T5663	T5661	nsubj	activation,causes
R333	T915	T913	prep	5,3
R3330	T5665	T5661	dobj	dissociation,causes
R3331	T5666	T5665	prep	of,dissociation
R3332	T5667	T5668	compound	HOMER,proteins
R3333	T5668	T5666	pobj	proteins,of
R3334	T5669	T5670	punct	[,35
R3335	T5670	T5661	parataxis	35,causes
R3336	T5671	T5670	punct	],35
R3337	T5672	T5664	punct	", ",released
R3338	T5673	T5664	nsubjpass	PAX6,released
R3339	T5674	T5664	aux	may,released
R334	T916	T913	punct	],3
R3340	T5675	T5664	auxpass	be,released
R3341	T5676	T5664	prep	as,released
R3342	T5677	T5678	det	a,result
R3343	T5678	T5676	pobj	result,as
R3344	T5679	T5678	prep	of,result
R3345	T5680	T5681	amod	synaptic,activity
R3346	T5681	T5679	pobj	activity,of
R3347	T5682	T5664	punct	", ",released
R3348	T5683	T5664	advcl	allowing,released
R3349	T5684	T5685	nsubj	it,interact
R335	T917	T896	punct	.,are
R3350	T5685	T5683	ccomp	interact,allowing
R3351	T5686	T5685	aux	to,interact
R3352	T5687	T5685	prep	with,interact
R3353	T5688	T5687	pobj	DNCL1,with
R3354	T5689	T5664	punct	.,released
R3355	T5691	T5692	det	The,complex
R3356	T5692	T5696	nsubj	complex,participate
R3357	T5693	T5694	compound	PAX6,DNCL1
R3358	T5694	T5692	compound	DNCL1,complex
R3359	T5695	T5694	punct	/,DNCL1
R336	T919	T920	amod	Homozygous,mutations
R3360	T5697	T5696	aux	could,participate
R3361	T5698	T5696	advmod	then,participate
R3362	T5699	T5696	prep	in,participate
R3363	T5700	T5701	compound	myosin,Va
R3364	T5701	T5702	npadvmod	Va,mediated
R3365	T5702	T5704	amod	mediated,transport
R3366	T5703	T5702	punct	-,mediated
R3367	T5704	T5699	pobj	transport,in
R3368	T5705	T5704	prep	along,transport
R3369	T5706	T5707	det	the,cytoskeleton
R337	T920	T921	nsubj	mutations,are
R3370	T5707	T5705	pobj	cytoskeleton,along
R3371	T5708	T5709	npadvmod	PSD,associated
R3372	T5709	T5707	amod	associated,cytoskeleton
R3373	T5710	T5709	punct	-,associated
R3374	T5711	T5707	compound	actin,cytoskeleton
R3375	T5712	T5704	acl	followed,transport
R3376	T5713	T5712	agent	by,followed
R3377	T5714	T5715	npadvmod	dynein,mediated
R3378	T5715	T5717	amod	mediated,transport
R3379	T5716	T5715	punct	-,mediated
R338	T922	T920	prep	in,mutations
R3380	T5717	T5713	pobj	transport,by
R3381	T5718	T5717	prep	along,transport
R3382	T5719	T5720	det	the,network
R3383	T5720	T5718	pobj	network,along
R3384	T5721	T5720	compound	microtubule,network
R3385	T5722	T5696	punct	", ",participate
R3386	T5723	T5724	advmod	eventually,reaching
R3387	T5724	T5696	advcl	reaching,participate
R3388	T5725	T5726	det	the,nucleus
R3389	T5726	T5724	dobj	nucleus,reaching
R339	T923	T922	pobj	man,in
R3390	T5727	T5728	punct	[,36
R3391	T5728	T5696	parataxis	36,participate
R3392	T5729	T5728	punct	],36
R3393	T5730	T5696	punct	.,participate
R3394	T5732	T5733	mark	Since,implicated
R3395	T5733	T5736	advcl	implicated,play
R3396	T5734	T5733	nsubjpass	TRIM11,implicated
R3397	T5735	T5733	auxpass	is,implicated
R3398	T5737	T5733	prep	in,implicated
R3399	T5738	T5739	compound	protein,degradation
R34	T215	T213	cc	and,ocular
R340	T924	T923	cc	and,man
R3400	T5739	T5737	pobj	degradation,in
R3401	T5740	T5741	punct	[,46
R3402	T5741	T5733	parataxis	46,implicated
R3403	T5742	T5741	punct	],46
R3404	T5743	T5736	punct	", ",play
R3405	T5744	T5736	nsubj	it,play
R3406	T5745	T5736	aux	may,play
R3407	T5746	T5747	det	a,role
R3408	T5747	T5736	dobj	role,play
R3409	T5748	T5736	prep	in,play
R341	T925	T923	conj	mouse,man
R3410	T5749	T5750	compound	PAX6,protein
R3411	T5750	T5751	compound	protein,turnover
R3412	T5751	T5748	pobj	turnover,in
R3413	T5752	T5736	punct	.,play
R3414	T5754	T5755	nsubj	Precedents,include
R3415	T5756	T5754	prep	for,Precedents
R3416	T5757	T5756	pobj	association,for
R3417	T5758	T5757	prep	of,association
R3418	T5759	T5760	compound	transcription,factors
R3419	T5760	T5758	pobj	factors,of
R342	T926	T921	acomp	lethal,are
R3420	T5761	T5757	prep	with,association
R3421	T5762	T5763	det	the,density
R3422	T5763	T5761	pobj	density,with
R3423	T5764	T5763	amod	post-synaptic,density
R3424	T5765	T5755	dobj	STAT3,include
R3425	T5766	T5765	cc	and,STAT3
R3426	T5767	T5765	conj	CREB,STAT3
R3427	T5768	T5765	punct	", ",STAT3
R3428	T5769	T5770	dep	which,act
R3429	T5770	T5765	relcl	act,STAT3
R343	T927	T921	cc	and,are
R3430	T5771	T5770	prep	as,act
R3431	T5772	T5771	pobj	messengers,as
R3432	T5773	T5772	prep	between,messengers
R3433	T5774	T5775	det	the,synapse
R3434	T5775	T5773	pobj	synapse,between
R3435	T5776	T5775	cc	and,synapse
R3436	T5777	T5778	det	the,nucleus
R3437	T5778	T5775	conj	nucleus,synapse
R3438	T5779	T5780	punct	[,48
R3439	T5780	T5755	parataxis	48,include
R344	T928	T921	conj	result,are
R3440	T5781	T5780	nummod	47,48
R3441	T5782	T5780	punct	",",48
R3442	T5783	T5780	punct	],48
R3443	T5784	T5755	punct	.,include
R3444	T5786	T5787	mark	Although,is
R3445	T5787	T5794	advcl	is,is
R3446	T5788	T5789	det	the,protein
R3447	T5789	T5787	nsubj	protein,is
R3448	T5790	T5791	amod	full,length
R3449	T5791	T5789	compound	length,protein
R345	T929	T928	prep	in,result
R3450	T5792	T5791	punct	-,length
R3451	T5793	T5789	compound	PAX6,protein
R3452	T5795	T5796	advmod	predominantly,nuclear
R3453	T5796	T5787	acomp	nuclear,is
R3454	T5797	T5794	punct	", ",is
R3455	T5798	T5794	expl	there,is
R3456	T5799	T5800	amod	good,evidence
R3457	T5800	T5794	attr	evidence,is
R3458	T5801	T5800	prep	in,evidence
R3459	T5802	T5801	pobj	mouse,in
R346	T930	T931	amod	severe,abnormalities
R3460	T5803	T5802	punct	", ",mouse
R3461	T5804	T5802	conj	quail,mouse
R3462	T5805	T5804	cc	and,quail
R3463	T5806	T5804	conj	nematode,quail
R3464	T5807	T5800	prep	for,evidence
R3465	T5808	T5809	det	an,isoform
R3466	T5809	T5807	pobj	isoform,for
R3467	T5810	T5811	dep	that,lacks
R3468	T5811	T5809	relcl	lacks,isoform
R3469	T5812	T5813	det	the,domain
R347	T931	T929	pobj	abnormalities,in
R3470	T5813	T5811	dobj	domain,lacks
R3471	T5814	T5813	amod	paired,domain
R3472	T5815	T5811	punct	", ",lacks
R3473	T5816	T5811	conj	is,lacks
R3474	T5817	T5818	preconj	both,nuclear
R3475	T5818	T5816	acomp	nuclear,is
R3476	T5819	T5818	cc	and,nuclear
R3477	T5820	T5818	conj	cytoplasmic,nuclear
R3478	T5821	T5816	punct	", ",is
R3479	T5822	T5816	cc	and,is
R348	T932	T931	amod	developmental,abnormalities
R3480	T5823	T5816	conj	binds,is
R3481	T5824	T5823	dobj	DNA,binds
R3482	T5825	T5823	prep	through,binds
R3483	T5826	T5827	det	the,homeodomain
R3484	T5827	T5825	pobj	homeodomain,through
R3485	T5828	T5827	advmod	alone,homeodomain
R3486	T5829	T5830	punct	[,49
R3487	T5830	T5794	parataxis	49,is
R3488	T5831	T5832	punct	-,51
R3489	T5832	T5830	prep	51,49
R349	T933	T931	prep	including,abnormalities
R3490	T5833	T5830	punct	],49
R3491	T5834	T5794	punct	.,is
R3492	T5836	T5837	prep	In,remains
R3493	T5838	T5836	pobj	mice,In
R3494	T5839	T5840	det	the,isoform
R3495	T5840	T5844	nsubj	isoform,is
R3496	T5841	T5842	advmod	paired,less
R3497	T5842	T5840	amod	less,isoform
R3498	T5843	T5842	punct	-,less
R3499	T5844	T5837	ccomp	is,remains
R35	T216	T213	conj	neurological,ocular
R350	T934	T933	pobj	anophthalmia,including
R3500	T5845	T5846	advmod	relatively,abundant
R3501	T5846	T5844	acomp	abundant,is
R3502	T5847	T5844	prep	in,is
R3503	T5848	T5847	pobj	brain,in
R3504	T5849	T5850	punct	[,49
R3505	T5850	T5844	parataxis	49,is
R3506	T5851	T5850	punct	],49
R3507	T5852	T5837	punct	;,remains
R3508	T5853	T5837	advmod	however,remains
R3509	T5854	T5855	poss	its,localisation
R351	T935	T934	punct	", ",anophthalmia
R3510	T5855	T5837	nsubj	localisation,remains
R3511	T5856	T5855	amod	subcellular,localisation
R3512	T5857	T5855	prep	in,localisation
R3513	T5858	T5857	pobj	neurones,in
R3514	T5859	T5855	punct	", ",localisation
R3515	T5860	T5855	cc	and,localisation
R3516	T5861	T5862	det	the,possibility
R3517	T5862	T5855	conj	possibility,localisation
R3518	T5863	T5862	prep	of,possibility
R3519	T5864	T5865	det	an,association
R352	T936	T937	amod	severe,reduction
R3520	T5865	T5863	pobj	association,of
R3521	T5866	T5865	prep	with,association
R3522	T5867	T5868	det	the,PSD
R3523	T5868	T5866	pobj	PSD,with
R3524	T5869	T5837	punct	", ",remains
R3525	T5870	T5871	aux	to,investigated
R3526	T5871	T5837	xcomp	investigated,remains
R3527	T5872	T5871	auxpass	be,investigated
R3528	T5873	T5837	punct	.,remains
R3529	T5875	T5876	prep	Regarding,found
R353	T937	T934	conj	reduction,anophthalmia
R3530	T5877	T5878	det	the,localisation
R3531	T5878	T5875	pobj	localisation,Regarding
R3532	T5879	T5878	amod	subcellular,localisation
R3533	T5880	T5878	prep	of,localisation
R3534	T5881	T5882	det	the,proteins
R3535	T5882	T5880	pobj	proteins,of
R3536	T5883	T5882	amod	putative,proteins
R3537	T5884	T5882	amod	interacting,proteins
R3538	T5885	T5876	punct	", ",found
R3539	T5886	T5876	nsubjpass	HOMER3,found
R354	T938	T937	prep	of,reduction
R3540	T5887	T5876	auxpass	is,found
R3541	T5888	T5876	advmod	predominantly,found
R3542	T5889	T5876	prep	at,found
R3543	T5890	T5891	det	the,interface
R3544	T5891	T5889	pobj	interface,at
R3545	T5892	T5891	prep	between,interface
R3546	T5893	T5894	det	the,PSD
R3547	T5894	T5892	pobj	PSD,between
R3548	T5895	T5894	cc	and,PSD
R3549	T5896	T5897	det	the,cytoplasm
R355	T939	T940	det	the,structures
R3550	T5897	T5894	conj	cytoplasm,PSD
R3551	T5898	T5899	punct	[,39
R3552	T5899	T5876	parataxis	39,found
R3553	T5900	T5899	punct	],39
R3554	T5901	T5876	punct	", ",found
R3555	T5902	T5903	nsubj	DNCL1,is
R3556	T5903	T5876	conj	is,found
R3557	T5904	T5905	advmod	chiefly,cytoplasmic
R3558	T5905	T5903	acomp	cytoplasmic,is
R3559	T5906	T5903	punct	", ",is
R356	T940	T938	pobj	structures,of
R3560	T5907	T5908	mark	although,reported
R3561	T5908	T5903	advcl	reported,is
R3562	T5909	T5910	amod	nuclear,localisation
R3563	T5910	T5908	nsubjpass	localisation,reported
R3564	T5911	T5908	aux	has,reported
R3565	T5912	T5908	auxpass	been,reported
R3566	T5913	T5914	punct	[,51
R3567	T5914	T5903	parataxis	51,is
R3568	T5960	T5952	auxpass	been,detected
R3569	T5915	T5914	punct	],51
R357	T941	T940	amod	olfactory,structures
R3570	T5961	T5952	prep	in,detected
R3571	T5916	T5903	punct	", ",is
R3572	T5962	T5961	pobj	thymus,in
R3573	T5917	T5903	cc	and,is
R3574	T5963	T5962	cc	and,thymus
R3575	T5964	T5962	conj	lung,thymus
R3576	T5918	T5919	nsubj	TRIM11,is
R3577	T5965	T5952	cc	but,detected
R3578	T5919	T5903	conj	is,is
R3579	T5920	T5921	preconj	both,nuclear
R358	T942	T937	cc	and,reduction
R3580	T5966	T5967	nsubjpass	it,studied
R3581	T5921	T5919	acomp	nuclear,is
R3582	T5967	T5952	conj	studied,detected
R3583	T5968	T5967	aux	has,studied
R3584	T5969	T5967	advmod	mainly,studied
R3585	T5922	T5921	cc	and,nuclear
R3586	T5970	T5967	auxpass	been,studied
R3587	T5971	T5967	prep	in,studied
R3588	T5923	T5921	conj	cytoplasmic,nuclear
R3589	T5972	T5971	pobj	brain,in
R359	T943	T944	amod	gross,malformations
R3590	T5973	T5974	advmod	where,found
R3591	T5974	T5972	relcl	found,brain
R3592	T5924	T5925	punct	[,38
R3593	T5975	T5974	nsubjpass	it,found
R3594	T5976	T5974	auxpass	is,found
R3595	T5925	T5919	parataxis	38,is
R3596	T5977	T5974	prep	in,found
R3597	T5978	T5979	det	the,forebrain
R3598	T5979	T5977	pobj	forebrain,in
R3599	T5980	T5979	punct	", ",forebrain
R36	T217	T204	punct	.,is
R360	T944	T937	conj	malformations,reduction
R3600	T5981	T5979	conj	hippocampus,forebrain
R3601	T5982	T5981	cc	and,hippocampus
R3602	T5926	T5925	punct	],38
R3603	T5983	T5981	conj	cerebellum,hippocampus
R3604	T5984	T5985	punct	[,39
R3605	T5985	T5967	parataxis	39,studied
R3606	T5927	T5876	punct	.,found
R3607	T5986	T5985	punct	],39
R3608	T5987	T5967	punct	.,studied
R3609	T5929	T5930	advmod	Therefore,interact
R361	T945	T944	compound	brain,malformations
R3610	T5989	T5990	nsubj	DNCL1,have
R3611	T5991	T5989	cc	and,DNCL1
R3612	T5931	T5932	amod	cytoplasmic,PAX6
R3613	T5992	T5989	conj	TRIM11,DNCL1
R3614	T5993	T5989	appos	both,DNCL1
R3615	T5994	T5995	amod	wide,domains
R3616	T5995	T5990	dobj	domains,have
R3617	T5996	T5995	compound	expression,domains
R3618	T5932	T5930	nsubj	PAX6,interact
R3619	T5997	T5998	dep	that,include
R362	T946	T947	punct	[,6
R3620	T5998	T5995	relcl	include,domains
R3621	T5999	T6000	det	the,brain
R3622	T5933	T5930	aux	could,interact
R3623	T6000	T5998	dobj	brain,include
R3624	T6001	T6002	punct	[,38
R3625	T5934	T5930	advmod	potentially,interact
R3626	T6002	T5990	parataxis	38,have
R3627	T6003	T6002	nummod	44,38
R3628	T6004	T6002	punct	",",38
R3629	T6005	T6002	punct	],38
R363	T947	T928	parataxis	6,result
R3630	T5935	T5930	prep	with,interact
R3631	T6006	T5990	punct	.,have
R3632	T5936	T5937	det	all,proteins
R3633	T6008	T6009	advmod	Thus,overlaps
R3634	T6010	T6011	det	the,expression
R3635	T5937	T5935	pobj	proteins,with
R3636	T6011	T6009	nsubj	expression,overlaps
R3637	T6012	T6011	prep	of,expression
R3638	T6013	T6014	det	all,interactors
R3639	T5938	T5937	nummod	three,proteins
R364	T948	T947	nummod	2,6
R3640	T6014	T6012	pobj	interactors,of
R3641	T6015	T6014	nummod	three,interactors
R3642	T6016	T6009	prep	with,overlaps
R3643	T6017	T6016	pobj	PAX6,with
R3644	T5939	T5930	punct	", ",interact
R3645	T6018	T6009	prep	at,overlaps
R3646	T6019	T6020	det	the,level
R3647	T5940	T5941	mark	while,interact
R3648	T6020	T6018	pobj	level,at
R3649	T6021	T6020	compound	tissue,level
R365	T949	T947	punct	",",6
R3650	T6022	T6023	punct	[,10
R3651	T6023	T6009	parataxis	10,overlaps
R3652	T6024	T6023	nummod	2,10
R3653	T6025	T6023	punct	",",10
R3654	T5941	T5930	advcl	interact,interact
R3655	T6026	T6023	nummod	7,10
R3656	T6027	T6023	punct	",",10
R3657	T6028	T6023	nummod	8,10
R3658	T5942	T5943	amod	nuclear,PAX6
R3659	T6029	T6023	punct	",",10
R366	T950	T947	punct	],6
R3660	T6030	T6023	punct	],10
R3661	T6031	T6009	punct	.,overlaps
R3662	T5943	T5941	nsubj	PAX6,interact
R3663	T6033	T6034	nsubj	We,detected
R3664	T5944	T5941	aux	could,interact
R3665	T6035	T6034	dobj	co-expression,detected
R3666	T5945	T5941	prep	with,interact
R3667	T6036	T6035	prep	of,co-expression
R3668	T6037	T6036	pobj	PAX6,of
R3669	T6038	T6037	punct	", ",PAX6
R367	T951	T921	punct	.,are
R3670	T5946	T5945	pobj	TRIM11,with
R3671	T6039	T6037	conj	HOMER3,PAX6
R3672	T5947	T5946	cc	and,TRIM11
R3673	T6040	T6039	punct	", ",HOMER3
R3674	T5948	T5946	conj	DNCL1,TRIM11
R3675	T6041	T6039	conj	DNCL1,HOMER3
R3676	T6042	T6041	cc	and,DNCL1
R3677	T5949	T5930	punct	.,interact
R3678	T6043	T6041	conj	TRIM11,DNCL1
R3679	T6044	T6034	prep	by,detected
R368	T953	T954	det	The,roles
R3680	T6045	T6046	compound	RT,PCR
R3681	T5951	T5952	prep	At,detected
R3682	T6046	T6044	pobj	PCR,by
R3683	T6047	T6046	punct	-,PCR
R3684	T6048	T6034	prep	in,detected
R3685	T5953	T5954	det	the,level
R3686	T6049	T6050	amod	human,RNA
R3687	T6050	T6048	pobj	RNA,in
R3688	T6051	T6050	amod	adult,RNA
R3689	T6052	T6050	compound	brain,RNA
R369	T954	T955	nsubjpass	roles,studied
R3690	T5954	T5951	pobj	level,At
R3691	T6053	T6054	punct	(,IH
R3692	T6054	T6034	parataxis	IH,detected
R3693	T6055	T6054	punct	", ",IH
R3694	T5955	T5954	compound	tissue,level
R3695	T6056	T6057	compound	L,Harrison
R3696	T6057	T6054	conj	Harrison,IH
R3697	T5956	T5952	punct	", ",detected
R3698	T6058	T6057	cc	and,Harrison
R3699	T6059	T6060	compound	A,Brown
R37	T219	T220	nsubj	Individuals,have
R370	T956	T954	prep	of,roles
R3700	T6060	T6057	conj	Brown,Harrison
R3701	T5957	T5958	compound	HOMER3,expression
R3702	T6061	T6054	punct	", ",IH
R3703	T6062	T6063	nsubj	data,shown
R3704	T6063	T6054	ccomp	shown,IH
R3705	T6064	T6063	neg	not,shown
R3706	T6065	T6054	punct	),IH
R3707	T5958	T5952	nsubjpass	expression,detected
R3708	T5959	T5952	aux	has,detected
R3709	T6172	T6171	dobj	proteins,generating
R371	T957	T956	pobj	PAX6,of
R3710	T6066	T6034	punct	.,detected
R3711	T6068	T6069	nsubj	We,demonstrated
R3712	T6173	T6172	prep	with,proteins
R3713	T6070	T6071	mark	that,impaired
R3714	T6071	T6069	ccomp	impaired,demonstrated
R3715	T6072	T6073	det	the,interaction
R3716	T6073	T6071	nsubjpass	interaction,impaired
R3717	T6174	T6175	amod	abnormal,extensions
R3718	T6074	T6073	prep	between,interaction
R3719	T6075	T6076	det	the,domain
R372	T958	T954	prep	in,roles
R3720	T6175	T6173	pobj	extensions,with
R3721	T6076	T6074	pobj	domain,between
R3722	T6077	T6076	compound	PST,domain
R3723	T6078	T6076	cc	and,domain
R3724	T6176	T6177	dep	that,expected
R3725	T6079	T6076	conj	HOMER3,domain
R3726	T6080	T6079	cc	or,HOMER3
R3727	T6081	T6079	conj	DNCL1,HOMER3
R3728	T6177	T6172	relcl	expected,proteins
R3729	T6082	T6071	auxpass	was,impaired
R373	T959	T960	compound	brain,development
R3730	T6083	T6071	agent	by,impaired
R3731	T6084	T6085	nummod	three,mutations
R3732	T6178	T6177	aux	might,expected
R3733	T6085	T6083	pobj	mutations,by
R3734	T6086	T6087	advmod	naturally,occurring
R3735	T6087	T6085	amod	occurring,mutations
R3736	T6179	T6177	auxpass	be,expected
R3737	T6088	T6089	dep	that,located
R3738	T6089	T6085	relcl	located,mutations
R3739	T6090	T6089	auxpass	are,located
R374	T960	T958	pobj	development,in
R3740	T6091	T6089	prep	in,located
R3741	T6180	T6181	aux	to,disrupt
R3742	T6092	T6093	det	the,peptide
R3743	T6093	T6091	pobj	peptide,in
R3744	T6181	T6177	xcomp	disrupt,expected
R3745	T6094	T6093	nmod	PAX6,peptide
R3746	T6095	T6096	npadvmod	C,terminal
R3747	T6096	T6093	amod	terminal,peptide
R3748	T6182	T6183	det	the,conformation
R3749	T6097	T6096	punct	-,terminal
R375	T961	T955	aux	have,studied
R3750	T6098	T6069	punct	.,demonstrated
R3751	T6183	T6181	dobj	conformation,disrupt
R3752	T6100	T6101	det	The,mutation
R3753	T6101	T6103	nsubj	mutation,caused
R3754	T6102	T6101	compound	Q422R,mutation
R3755	T6104	T6101	punct	", ",mutation
R3756	T6105	T6106	dep	which,involves
R3757	T6184	T6183	prep	of,conformation
R3758	T6106	T6101	relcl	involves,mutation
R3759	T6107	T6108	det	a,substitution
R376	T962	T955	advmod	mainly,studied
R3760	T6108	T6106	dobj	substitution,involves
R3761	T6185	T6186	det	the,end
R3762	T6109	T6108	nmod	glutamine,substitution
R3763	T6110	T6109	prep	to,glutamine
R3764	T6186	T6184	pobj	end,of
R3765	T6111	T6110	pobj	arginine,to
R3766	T6112	T6108	prep	at,substitution
R3767	T6113	T6114	det	the,position
R3768	T6187	T6188	npadvmod	C,terminal
R3769	T6114	T6112	pobj	position,at
R377	T963	T955	auxpass	been,studied
R3770	T6115	T6114	amod	last,position
R3771	T6116	T6117	compound	amino,acid
R3772	T6117	T6114	compound	acid,position
R3773	T6188	T6186	amod	terminal,end
R3774	T6118	T6114	prep	of,position
R3775	T6119	T6118	pobj	PAX6,of
R3776	T6189	T6188	punct	-,terminal
R3777	T6120	T6103	punct	", ",caused
R3778	T6121	T6122	det	a,reduction
R3779	T6122	T6103	dobj	reduction,caused
R378	T964	T955	prep	in,studied
R3780	T6123	T6122	prep	in,reduction
R3781	T6190	T6186	prep	of,end
R3782	T6124	T6125	det	the,interaction
R3783	T6191	T6192	det	the,protein
R3784	T6125	T6123	pobj	interaction,in
R3785	T6126	T6125	prep	with,interaction
R3786	T6127	T6126	pobj	HOMER3,with
R3787	T6192	T6190	pobj	protein,of
R3788	T6128	T6127	cc	and,HOMER3
R3789	T6129	T6127	conj	DNCL1,HOMER3
R379	T965	T966	amod	homozygous,mice
R3790	T6130	T6103	punct	.,caused
R3791	T6193	T6153	punct	.,is
R3792	T6132	T6133	det	The,mutations
R3793	T6195	T6196	det	The,mutation
R3794	T6133	T6137	nsubj	mutations,reduced
R3795	T6134	T6133	nmod	X423L,mutations
R3796	T6135	T6134	cc	and,X423L
R3797	T6136	T6134	conj	1615del10,X423L
R3798	T6196	T6198	nsubj	mutation,removes
R3799	T6138	T6137	advmod	severely,reduced
R38	T221	T219	prep	with,Individuals
R380	T966	T964	pobj	mice,in
R3800	T6139	T6137	cc	or,reduced
R3801	T6197	T6196	compound	1615del10,mutation
R3802	T6140	T6141	advmod	completely,abolished
R3803	T6141	T6137	conj	abolished,reduced
R3804	T6142	T6143	det	the,interaction
R3805	T6199	T6198	advmod	also,removes
R3806	T6143	T6141	dobj	interaction,abolished
R3807	T6144	T6143	prep	with,interaction
R3808	T6145	T6144	pobj	HOMER3,with
R3809	T6146	T6145	cc	and,HOMER3
R381	T967	T966	compound	mutant,mice
R3810	T6147	T6145	conj	DNCL1,HOMER3
R3811	T6148	T6137	punct	.,reduced
R3812	T6200	T6201	det	the,acids
R3813	T6150	T6151	det	The,effect
R3814	T6151	T6153	nsubj	effect,is
R3815	T6152	T6151	amod	predicted,effect
R3816	T6201	T6198	dobj	acids,removes
R3817	T6154	T6151	prep	of,effect
R3818	T6155	T6156	det	the,mutations
R3819	T6202	T6201	amod	last,acids
R382	T968	T966	cc	or,mice
R3820	T6156	T6154	pobj	mutations,of
R3821	T6157	T6156	nmod	X423L,mutations
R3822	T6203	T6201	nummod	5,acids
R3823	T6158	T6157	cc	and,X423L
R3824	T6159	T6157	conj	1615del10,X423L
R3825	T6160	T6161	aux	to,cause
R3826	T6204	T6201	compound	amino,acids
R3827	T6161	T6153	xcomp	cause,is
R3828	T6162	T6161	dobj	translation,cause
R3829	T6205	T6201	prep	of,acids
R383	T969	T966	conj	rats,mice
R3830	T6163	T6162	prep	into,translation
R3831	T6164	T6165	det	the,region
R3832	T6165	T6163	pobj	region,into
R3833	T6206	T6207	det	the,peptide
R3834	T6166	T6165	nummod	3,region
R3835	T6167	T6166	punct	',3
R3836	T6168	T6165	amod	untranslated,region
R3837	T6169	T6161	punct	", ",cause
R3838	T6207	T6205	pobj	peptide,of
R3839	T6170	T6171	advmod	thus,generating
R384	T970	T955	cc	and,studied
R3840	T6171	T6161	advcl	generating,cause
R3841	T6208	T6209	npadvmod	C,terminal
R3842	T6209	T6207	amod	terminal,peptide
R3843	T6210	T6209	punct	-,terminal
R3844	T6278	T6275	conj	peptide,homeodomain
R3845	T6279	T6280	npadvmod	C,terminal
R3846	T6211	T6212	punct	[,28
R3847	T6280	T6278	amod	terminal,peptide
R3848	T6281	T6280	punct	-,terminal
R3849	T6282	T6250	punct	.,are
R385	T971	T955	conj	include,studied
R3850	T6212	T6198	parataxis	28,removes
R3851	T6284	T6285	amod	Most,patients
R3852	T6285	T6287	nsubj	patients,are
R3853	T6213	T6212	punct	],28
R3854	T6286	T6285	compound	aniridia,patients
R3855	T6288	T6287	acomp	heterozygous,are
R3856	T6214	T6198	punct	.,removes
R3857	T6289	T6288	prep	for,heterozygous
R3858	T6290	T6289	pobj	mutations,for
R3859	T6291	T6292	dep	that,introduce
R386	T972	T971	dobj	arealisation,include
R3860	T6292	T6290	relcl	introduce,mutations
R3861	T6293	T6294	det	a,codon
R3862	T6294	T6292	dobj	codon,introduce
R3863	T6216	T6217	nsubj	None,affected
R3864	T6295	T6296	amod	premature,termination
R3865	T6296	T6294	compound	termination,codon
R3866	T6297	T6292	prep	into,introduce
R3867	T6298	T6299	det	the,frame
R3868	T6218	T6216	prep	of,None
R3869	T6299	T6297	pobj	frame,into
R387	T973	T972	prep	of,arealisation
R3870	T6219	T6220	det	the,mutations
R3871	T6300	T6299	nmod	PAX6,frame
R3872	T6220	T6218	pobj	mutations,of
R3873	T6301	T6302	amod	open,reading
R3874	T6302	T6299	compound	reading,frame
R3875	T6303	T6304	punct	[,52
R3876	T6221	T6222	det	the,interaction
R3877	T6304	T6287	parataxis	52,are
R3878	T6305	T6304	nummod	2,52
R3879	T6306	T6304	punct	",",52
R388	T974	T975	det	the,cortex
R3880	T6222	T6217	dobj	interaction,affected
R3881	T6307	T6304	punct	],52
R3882	T6223	T6222	prep	with,interaction
R3883	T6308	T6287	punct	.,are
R3884	T6310	T6311	det	These,alleles
R3885	T6224	T6223	pobj	TRIM11,with
R3886	T6311	T6312	nsubjpass	alleles,expected
R3887	T6313	T6312	aux	would,expected
R3888	T6225	T6217	punct	", ",affected
R3889	T6314	T6312	auxpass	be,expected
R389	T975	T973	pobj	cortex,of
R3890	T6315	T6316	aux	to,encode
R3891	T6316	T6312	xcomp	encode,expected
R3892	T6226	T6217	advcl	suggesting,affected
R3893	T6317	T6318	amod	truncated,proteins
R3894	T6318	T6316	dobj	proteins,encode
R3895	T6319	T6318	cc	or,proteins
R3896	T6227	T6228	mark	that,alter
R3897	T6320	T6321	det	no,protein
R3898	T6321	T6318	conj	protein,proteins
R3899	T6322	T6323	advmod	at,all
R39	T222	T223	amod	heterozygous,mutations
R390	T976	T975	amod	cerebral,cortex
R3900	T6228	T6226	ccomp	alter,suggesting
R3901	T6323	T6321	advmod	all,protein
R3902	T6324	T6325	mark	if,degraded
R3903	T6229	T6228	nsubj	they,alter
R3904	T6325	T6316	advcl	degraded,encode
R3905	T6326	T6327	det	the,RNA
R3906	T6230	T6228	aux	do,alter
R3907	T6327	T6325	nsubjpass	RNA,degraded
R3908	T6328	T6327	compound	mutant,RNA
R3909	T6329	T6325	auxpass	is,degraded
R391	T977	T978	punct	[,7
R3910	T6330	T6325	agent	by,degraded
R3911	T6331	T6332	npadvmod	nonsense,mediated
R3912	T6332	T6334	amod	mediated,decay
R3913	T6333	T6332	punct	-,mediated
R3914	T6231	T6228	neg	not,alter
R3915	T6334	T6330	pobj	decay,by
R3916	T6335	T6336	punct	[,52
R3917	T6336	T6312	parataxis	52,expected
R3918	T6337	T6336	punct	],52
R3919	T6232	T6233	det	the,conformation
R392	T978	T972	parataxis	7,arealisation
R3920	T6338	T6312	punct	.,expected
R3921	T6233	T6228	dobj	conformation,alter
R3922	T6340	T6341	nsubj	We,propose
R3923	T6342	T6343	mark	that,explained
R3924	T6234	T6233	prep	of,conformation
R3925	T6343	T6341	ccomp	explained,propose
R3926	T6344	T6345	det	the,anomalies
R3927	T6235	T6236	det	the,part
R3928	T6345	T6343	nsubjpass	anomalies,explained
R3929	T6346	T6345	compound	brain,anomalies
R393	T979	T978	punct	],7
R3930	T6347	T6348	dep	that,observed
R3931	T6236	T6234	pobj	part,of
R3932	T6348	T6345	relcl	observed,anomalies
R3933	T6349	T6348	aux	have,observed
R3934	T6350	T6348	auxpass	been,observed
R3935	T6237	T6238	advmod	more,terminal
R3936	T6351	T6348	prep	in,observed
R3937	T6352	T6353	compound	aniridia,patients
R3938	T6238	T6236	amod	terminal,part
R3939	T6353	T6351	pobj	patients,in
R394	T980	T972	punct	", ",arealisation
R3940	T6354	T6343	aux	may,explained
R3941	T6355	T6343	auxpass	be,explained
R3942	T6356	T6343	advmod	partly,explained
R3943	T6239	T6238	npadvmod	N,terminal
R3944	T6357	T6343	agent	by,explained
R3945	T6240	T6238	punct	-,terminal
R3946	T6241	T6236	prep	of,part
R3947	T6358	T6359	amod	impaired,interaction
R3948	T6242	T6243	det	the,domain
R3949	T6359	T6357	pobj	interaction,by
R395	T981	T972	conj	formation,arealisation
R3950	T6360	T6359	prep	between,interaction
R3951	T6361	T6360	pobj	PAX6,between
R3952	T6243	T6241	pobj	domain,of
R3953	T6362	T6361	cc	and,PAX6
R3954	T6363	T6361	conj	HOMER3,PAX6
R3955	T6364	T6361	punct	", ",PAX6
R3956	T6244	T6243	compound	PST,domain
R3957	T6365	T6361	appos	DNCL1,PAX6
R3958	T6366	T6365	cc	and,DNCL1
R3959	T6367	T6365	conj	TRIM11,DNCL1
R396	T982	T981	prep	of,formation
R3960	T6245	T6217	punct	.,affected
R3961	T6368	T6341	punct	.,propose
R3962	T6247	T6248	predet	All,data
R3963	T6370	T6371	det	The,phenotype
R3964	T6371	T6373	nsubj	phenotype,result
R3965	T6372	T6371	amod	neurobehavioural,phenotype
R3966	T6374	T6371	acl	associated,phenotype
R3967	T6375	T6374	prep	with,associated
R3968	T6376	T6375	pobj	1615del10,with
R3969	T6248	T6250	nsubj	data,are
R397	T983	T984	det	the,boundary
R3970	T6377	T6371	cc	and,phenotype
R3971	T6378	T6379	det	the,polymicrogyria
R3972	T6379	T6371	conj	polymicrogyria,phenotype
R3973	T6249	T6248	poss	our,data
R3974	T6380	T6379	acl	associated,polymicrogyria
R3975	T6381	T6380	prep	with,associated
R3976	T6382	T6381	pobj	X423L,with
R3977	T6251	T6250	acomp	consistent,are
R3978	T6383	T6373	aux	may,result
R3979	T6252	T6251	prep	with,consistent
R398	T984	T982	pobj	boundary,of
R3980	T6253	T6254	det	the,hypothesis
R3981	T6254	T6252	pobj	hypothesis,with
R3982	T6384	T6373	prep	from,result
R3983	T6385	T6386	det	a,effect
R3984	T6255	T6256	mark	that,interact
R3985	T6256	T6254	acl	interact,hypothesis
R3986	T6386	T6384	pobj	effect,from
R3987	T6257	T6256	nsubj	TRIM11,interact
R3988	T6387	T6386	amod	specific,effect
R3989	T6388	T6386	prep	of,effect
R399	T985	T986	compound	prosencephalon,mesencephalon
R3990	T6258	T6256	aux	does,interact
R3991	T6389	T6390	det	these,mutations
R3992	T6390	T6388	pobj	mutations,of
R3993	T6259	T6256	neg	not,interact
R3994	T6391	T6390	amod	unusual,mutations
R3995	T6392	T6393	npadvmod	C,terminal
R3996	T6260	T6256	prep	with,interact
R3997	T6393	T6390	amod	terminal,mutations
R3998	T6394	T6393	punct	-,terminal
R3999	T6261	T6262	det	the,peptide
R4	T180	T179	pobj	proteins,for
R40	T223	T221	pobj	mutations,with
R400	T986	T984	compound	mesencephalon,boundary
R4000	T6395	T6390	compound	extension,mutations
R4001	T6396	T6373	punct	.,result
R4002	T6262	T6260	pobj	peptide,with
R4003	T6398	T6399	expl	There,is
R4004	T6400	T6399	attr	evidence,is
R4005	T6263	T6264	npadvmod	C,terminal
R4006	T6401	T6402	mark	that,conserved
R4007	T6402	T6400	acl	conserved,evidence
R4008	T6264	T6262	amod	terminal,peptide
R4009	T6403	T6404	nmod	signalling,mechanisms
R401	T987	T986	punct	-,mesencephalon
R4010	T6404	T6402	nsubjpass	mechanisms,conserved
R4011	T6405	T6403	cc	and,signalling
R4012	T6265	T6264	punct	-,terminal
R4013	T6406	T6403	conj	transport,signalling
R4014	T6407	T6408	dep	that,characterized
R4015	T6408	T6404	relcl	characterized,mechanisms
R4016	T6409	T6408	auxpass	were,characterized
R4017	T6410	T6408	advmod	initially,characterized
R4018	T6411	T6408	prep	in,characterized
R4019	T6266	T6256	punct	", ",interact
R402	T988	T989	punct	[,8
R4020	T6412	T6413	det	the,brain
R4021	T6413	T6411	pobj	brain,in
R4022	T6414	T6402	aux	may,conserved
R4023	T6267	T6256	cc	but,interact
R4024	T6415	T6402	advmod	also,conserved
R4025	T6416	T6402	auxpass	be,conserved
R4026	T6268	T6256	conj	interacts,interact
R4027	T6417	T6402	prep	in,conserved
R4028	T6418	T6419	det	the,retina
R4029	T6419	T6417	pobj	retina,in
R403	T989	T981	parataxis	8,formation
R4030	T6269	T6268	prep	with,interacts
R4031	T6420	T6402	punct	", ",conserved
R4032	T6421	T6402	advcl	suggesting,conserved
R4033	T6422	T6423	mark	that,have
R4034	T6270	T6271	det	the,domain
R4035	T6423	T6421	ccomp	have,suggesting
R4036	T6424	T6425	amod	impaired,interactions
R4037	T6425	T6423	nsubj	interactions,have
R4038	T6271	T6269	pobj	domain,with
R4039	T6426	T6425	nmod	PAX6,interactions
R404	T990	T989	punct	],8
R4040	T6427	T6428	nmod	protein,protein
R4041	T6272	T6271	compound	PST,domain
R4042	T6428	T6425	compound	protein,interactions
R4043	T6429	T6428	punct	-,protein
R4044	T6430	T6423	aux	may,have
R4045	T6273	T6271	prep	between,domain
R4046	T6431	T6423	advmod	also,have
R4047	T6432	T6423	dobj	implications,have
R4048	T6433	T6432	prep	for,implications
R4049	T6274	T6275	det	the,homeodomain
R405	T991	T981	punct	", ",formation
R4050	T6434	T6435	det	the,defects
R4051	T6435	T6433	pobj	defects,for
R4052	T6436	T6435	amod	retinal,defects
R4053	T6275	T6273	pobj	homeodomain,between
R4054	T6437	T6435	acl	observed,defects
R4055	T6438	T6437	prep	in,observed
R4056	T6439	T6438	pobj	individuals,in
R4057	T6276	T6275	cc	and,homeodomain
R4058	T6440	T6439	prep	with,individuals
R4059	T6441	T6442	compound	PAX6,mutations
R406	T992	T993	compound	axon,guidance
R4060	T6277	T6278	det	the,peptide
R4061	T6442	T6440	pobj	mutations,with
R4062	T6443	T6444	punct	[,54
R4063	T6444	T6399	parataxis	54,is
R4064	T6445	T6444	nummod	53,54
R4065	T6446	T6444	punct	",",54
R4066	T6447	T6444	punct	],54
R4067	T6448	T6399	punct	.,is
R4068	T6504	T6505	nsubj	We,presented
R4069	T6506	T6505	aux	have,presented
R407	T993	T981	conj	guidance,formation
R4070	T6507	T6508	amod	preliminary,evidence
R4071	T6508	T6505	dobj	evidence,presented
R4072	T6509	T6510	mark	that,interacts
R4073	T6510	T6508	acl	interacts,evidence
R4074	T6511	T6512	det	the,regulator
R4075	T6512	T6510	nsubj	regulator,interacts
R4076	T6513	T6512	amod	neurodevelopmental,regulator
R4077	T6514	T6512	amod	transcriptional,regulator
R4078	T6515	T6512	appos	PAX6,regulator
R4079	T6516	T6510	prep	with,interacts
R408	T994	T995	punct	[,8
R4080	T6517	T6516	pobj	HOMER3,with
R4081	T6518	T6517	punct	", ",HOMER3
R4082	T6519	T6517	conj	DNCL1,HOMER3
R4083	T6520	T6519	cc	and,DNCL1
R4084	T6521	T6519	conj	TRIM11,DNCL1
R4085	T6522	T6505	punct	.,presented
R4086	T6524	T6525	nsubj	We,suggest
R4087	T6526	T6527	mark	that,is
R4088	T6527	T6525	ccomp	is,suggest
R4089	T6528	T6529	det	the,interaction
R409	T995	T993	parataxis	8,guidance
R4090	T6529	T6527	nsubj	interaction,is
R4091	T6530	T6529	prep	of,interaction
R4092	T6531	T6530	pobj	PAX6,of
R4093	T6532	T6529	prep	with,interaction
R4094	T6533	T6532	pobj	HOMER3,with
R4095	T6534	T6533	cc	and,HOMER3
R4096	T6535	T6533	conj	DNCL1,HOMER3
R4097	T6536	T6537	det	a,mechanism
R4098	T6537	T6527	attr	mechanism,is
R4099	T6538	T6539	prep	by,lead
R41	T224	T223	nmod	loss,mutations
R410	T996	T995	punct	],8
R4100	T6539	T6537	relcl	lead,mechanism
R4101	T6540	T6538	pobj	which,by
R4102	T6541	T6542	amod	synaptic,signalling
R4103	T6542	T6539	nsubj	signalling,lead
R4104	T6543	T6539	aux	could,lead
R4105	T6544	T6539	prep	to,lead
R4106	T6545	T6546	amod	regulated,changes
R4107	T6546	T6544	pobj	changes,to
R4108	T6547	T6546	prep	in,changes
R4109	T6548	T6549	compound	gene,expression
R411	T997	T993	punct	", ",guidance
R4110	T6549	T6547	pobj	expression,in
R4111	T6550	T6539	prep	in,lead
R4112	T6551	T6550	pobj	neurons,in
R4113	T6552	T6525	punct	.,suggest
R4114	T6554	T6555	nsubj	We,propose
R4115	T6556	T6555	advmod	also,propose
R4116	T6557	T6558	mark	that,explained
R4117	T6558	T6555	ccomp	explained,propose
R4118	T6559	T6558	nsubjpass	some,explained
R4119	T6560	T6559	prep	of,some
R412	T998	T993	conj	differentiation,guidance
R4120	T6561	T6562	det	the,anomalies
R4121	T6562	T6560	pobj	anomalies,of
R4122	T6563	T6562	amod	neural,anomalies
R4123	T6564	T6562	prep	in,anomalies
R4124	T6565	T6564	pobj	patients,in
R4125	T6566	T6565	prep	with,patients
R4126	T6567	T6568	compound	PAX6,mutations
R4127	T6568	T6566	pobj	mutations,with
R4128	T6569	T6558	aux	may,explained
R4129	T6570	T6558	auxpass	be,explained
R413	T999	T998	prep	of,differentiation
R4130	T6571	T6558	agent	by,explained
R4131	T6572	T6573	amod	impaired,interactions
R4132	T6573	T6571	pobj	interactions,by
R4133	T6574	T6575	nmod	protein,protein
R4134	T6575	T6573	compound	protein,interactions
R4135	T6576	T6575	punct	-,protein
R4136	T6577	T6555	punct	.,propose
R4137	T6595	T6596	compound	Bioinformatics,techniques
R4138	T6598	T6599	compound	Sequence,searches
R4139	T6599	T6601	nsubjpass	searches,carried
R414	T1000	T999	pobj	neurons,of
R4140	T6600	T6599	compound	database,searches
R4141	T6602	T6601	auxpass	were,carried
R4142	T6603	T6601	prt	out,carried
R4143	T6604	T6601	advcl	using,carried
R4144	T6605	T6606	det	the,program
R4145	T6606	T6604	dobj	program,using
R4146	T6607	T6606	compound	BLAST,program
R4147	T6608	T6606	amod	available,program
R4148	T6609	T6608	prep	through,available
R4149	T6610	T6611	det	the,Applications
R415	T1001	T998	prep	from,differentiation
R4150	T6611	T6609	pobj	Applications,through
R4151	T6612	T6611	compound	Bioinformatics,Applications
R4152	T6613	T6611	prep	at,Applications
R4153	T6614	T6615	det	the,Centre
R4154	T6615	T6613	pobj	Centre,at
R4155	T6616	T6617	compound	Rosalind,Franklin
R4156	T6617	T6615	compound	Franklin,Centre
R4157	T6618	T6615	prep	for,Centre
R4158	T6619	T6620	compound	Genomics,Research
R4159	T6620	T6618	pobj	Research,for
R416	T1002	T1001	pobj	glia,from
R4160	T6621	T6622	punct	[,24
R4161	T6622	T6601	parataxis	24,carried
R4162	T6623	T6622	punct	],24
R4163	T6624	T6601	punct	.,carried
R4164	T6626	T6627	compound	Protein,sequences
R4165	T6627	T6628	nsubjpass	sequences,aligned
R4166	T6629	T6630	dep	that,were
R4167	T6630	T6627	relcl	were,sequences
R4168	T6631	T6632	advmod	highly,homologous
R4169	T6632	T6630	acomp	homologous,were
R417	T1003	T1004	punct	[,9
R4170	T6633	T6632	prep	to,homologous
R4171	T6634	T6635	det	the,terminus
R4172	T6635	T6633	pobj	terminus,to
R4173	T6636	T6635	compound	C,terminus
R4174	T6637	T6635	punct	-,terminus
R4175	T6638	T6635	prep	of,terminus
R4176	T6639	T6640	amod	human,PAX6
R4177	T6640	T6638	pobj	PAX6,of
R4178	T6641	T6628	auxpass	were,aligned
R4179	T6642	T6628	advcl	using,aligned
R418	T1004	T998	parataxis	9,differentiation
R4180	T6643	T6642	dobj	CLUSTAL,using
R4181	T6644	T6645	punct	[,24
R4182	T6645	T6628	parataxis	24,aligned
R4183	T6646	T6645	punct	],24
R4184	T6647	T6628	punct	.,aligned
R4185	T6649	T6650	amod	Secondary,structure
R4186	T6650	T6651	compound	structure,prediction
R4187	T6651	T6652	nsubjpass	prediction,performed
R4188	T6653	T6652	auxpass	was,performed
R4189	T6654	T6652	advcl	using,performed
R419	T1005	T1004	punct	],9
R4190	T6655	T6656	det	the,method
R4191	T6656	T6654	dobj	method,using
R4192	T6657	T6656	compound	JPRED,method
R4193	T6658	T6656	compound	consensus,method
R4194	T6659	T6660	punct	[,26
R4195	T6660	T6652	parataxis	26,performed
R4196	T6661	T6660	nummod	25,26
R4197	T6662	T6660	punct	",",26
R4198	T6663	T6660	punct	],26
R4199	T6664	T6652	punct	.,performed
R42	T225	T224	punct	-,loss
R420	T1006	T998	cc	and,differentiation
R4202	T6808	T6809	nmod	Yeast,constructs
R4203	T6810	T6811	nummod	two,hybrid
R4204	T6811	T6809	compound	hybrid,constructs
R4205	T6812	T6811	punct	-,hybrid
R4206	T6814	T6815	det	All,numbering
R4207	T6815	T6820	nsubj	numbering,is
R4208	T6816	T6815	nmod	cDNA,numbering
R4209	T6817	T6816	cc	and,cDNA
R421	T1007	T1008	amod	neuronal,migration
R4210	T6818	T6819	compound	amino,acid
R4211	T6819	T6816	conj	acid,cDNA
R4212	T6821	T6820	prep	based,is
R4213	T6822	T6821	prep	on,based
R4214	T6823	T6824	det	the,sequences
R4215	T6824	T6822	pobj	sequences,on
R4216	T6825	T6826	amod	human,cDNA
R4217	T6826	T6824	nmod	cDNA,sequences
R4218	T6827	T6826	nmod	PAX6,cDNA
R4219	T6828	T6826	cc	and,cDNA
R422	T1008	T998	conj	migration,differentiation
R4220	T6829	T6830	compound	protein,reference
R4221	T6830	T6826	conj	reference,cDNA
R4222	T6831	T6824	amod	available,sequences
R4223	T6832	T6831	prep	from,available
R4224	T6833	T6834	det	the,site
R4225	T6834	T6832	pobj	site,from
R4226	T6835	T6836	amod	Human,PAX6
R4227	T6836	T6837	nmod	PAX6,Database
R4228	T6837	T6834	compound	Database,site
R4229	T6838	T6839	amod	Allelic,Variant
R423	T1009	T1008	prep	in,migration
R4230	T6839	T6837	compound	Variant,Database
R4231	T6840	T6834	compound	web,site
R4232	T6841	T6842	punct	[,27
R4233	T6842	T6821	parataxis	27,based
R4234	T6843	T6842	punct	],27
R4235	T6844	T6820	punct	.,is
R4236	T6846	T6847	amod	Standard,techniques
R4237	T6847	T6851	nsubjpass	techniques,used
R4238	T6848	T6847	nmod	PCR,techniques
R4239	T6849	T6848	cc	and,PCR
R424	T1010	T1011	det	the,cerebellum
R4240	T6850	T6848	conj	subcloning,PCR
R4241	T6852	T6851	auxpass	were,used
R4242	T6853	T6854	aux	to,make
R4243	T6854	T6851	advcl	make,used
R4244	T6855	T6856	nummod	three,constructs
R4245	T6856	T6854	dobj	constructs,make
R4246	T6857	T6858	compound	PAX6,cDNA
R4247	T6858	T6856	compound	cDNA,constructs
R4248	T6859	T6854	prep	in,make
R4249	T6860	T6861	det	the,vector
R425	T1011	T1009	pobj	cerebellum,in
R4250	T6861	T6859	pobj	vector,in
R4251	T6862	T6861	compound	pDBLeu,vector
R4252	T6863	T6861	compound	expression,vector
R4253	T6864	T6865	punct	(,System
R4254	T6865	T6861	parataxis	System,vector
R4255	T6866	T6865	nmod	ProQuest,System
R4256	T6867	T6868	nummod	Two,Hybrid
R4257	T6868	T6865	compound	Hybrid,System
R4258	T6869	T6868	punct	-,Hybrid
R4259	T6870	T6865	punct	", ",System
R426	T1012	T1013	punct	[,10
R4260	T6871	T6865	npadvmod	Invitrogen,System
R4261	T6872	T6865	punct	),System
R4262	T6873	T6861	punct	", ",vector
R4263	T6874	T6875	dep	which,generates
R4264	T6875	T6861	relcl	generates,vector
R4265	T6876	T6877	det	a,protein
R4266	T6877	T6875	dobj	protein,generates
R4267	T6878	T6877	acl	fused,protein
R4268	T6879	T6878	prep	to,fused
R4269	T6880	T6881	det	the,domain
R427	T1013	T1008	parataxis	10,migration
R4270	T6881	T6879	pobj	domain,to
R4271	T6882	T6881	compound	yeast,domain
R4272	T6883	T6881	compound	GAL4,domain
R4273	T6884	T6885	compound	DNA,binding
R4274	T6885	T6881	compound	binding,domain
R4275	T6886	T6851	punct	.,used
R4276	T6888	T6889	nsubj	PAX6PST,contains
R4277	T6890	T6891	det	the,domain
R4278	T6891	T6889	dobj	domain,contains
R4279	T6892	T6891	amod	whole,domain
R428	T1014	T1013	punct	],10
R4280	T6893	T6891	compound	PST,domain
R4281	T6894	T6895	punct	(,278
R4282	T6895	T6889	parataxis	278,contains
R4283	T6896	T6895	nmod	amino,278
R4284	T6897	T6895	nmod	acids,278
R4285	T6898	T6899	punct	–,422
R4286	T6899	T6895	prep	422,278
R4287	T6900	T6895	prep	of,278
R4288	T6901	T6902	det	the,protein
R4289	T6902	T6900	pobj	protein,of
R429	T1015	T955	punct	.,studied
R4290	T6903	T6904	amod	full,length
R4291	T6904	T6902	compound	length,protein
R4292	T6905	T6904	punct	-,length
R4293	T6906	T6902	compound	PAX6,protein
R4294	T6907	T6895	punct	),278
R4295	T6908	T6889	punct	.,contains
R4296	T6910	T6911	nsubj	Primers,were
R4297	T6912	T6913	nmod	ST001,C
R4298	T6913	T6911	attr	C,were
R4299	T6914	T6915	punct	(,forward
R43	T226	T224	prep	of,loss
R430	T1017	T1018	det	The,discovery
R4300	T6915	T6913	parataxis	forward,C
R4301	T6916	T6915	punct	),forward
R4302	T6917	T6913	nummod	5,C
R4303	T6918	T6917	punct	',5
R4304	T6919	T6913	punct	-,C
R4305	T6920	T6913	compound	AAA,C
R4306	T6921	T6913	compound	AGT,C
R4307	T6922	T6913	compound	TCG,C
R4308	T6923	T6913	compound	ACT,C
R4309	T6924	T6913	compound	GCC,C
R431	T1018	T1019	nsubj	discovery,prompted
R4310	T6925	T6913	compound	AGC,C
R4311	T6926	T6913	compound	AAC,C
R4312	T6927	T6913	compound	ACA,C
R4313	T6928	T6913	compound	CCT,C
R4314	T6929	T6913	compound	AGT,C
R4315	T6930	T6913	punct	-,C
R4316	T6931	T6913	nummod	3,C
R4317	T6932	T6913	punct	',C
R4318	T6933	T6913	cc	and,C
R4319	T6934	T6935	nmod	ST005,TTG
R432	T1020	T1018	prep	of,discovery
R4320	T6935	T6913	conj	TTG,C
R4321	T6936	T6937	punct	(,R
R4322	T6937	T6935	parataxis	R,TTG
R4323	T6938	T6937	punct	),R
R4324	T6939	T6935	nummod	5,TTG
R4325	T6940	T6939	punct	',5
R4326	T6941	T6935	punct	-,TTG
R4327	T6942	T6935	compound	TTT,TTG
R4328	T6943	T6935	compound	TGC,TTG
R4329	T6944	T6935	compound	GGC,TTG
R433	T1021	T1022	amod	multiple,roles
R4330	T6945	T6935	compound	TTT,TTG
R4331	T6946	T6935	compound	TTA,TTG
R4332	T6947	T6935	compound	CTG,TTG
R4333	T6948	T6935	compound	TAA,TTG
R4334	T6949	T6935	compound	TCT,TTG
R4335	T6950	T6935	compound	TGG,TTG
R4336	T6951	T6935	compound	CCA,TTG
R4337	T6952	T6935	compound	GTA,TTG
R4338	T6953	T6935	punct	-,TTG
R4339	T6954	T6935	nummod	3,TTG
R434	T1022	T1020	pobj	roles,of
R4340	T6955	T6935	punct	',TTG
R4341	T6956	T6911	punct	.,were
R4342	T6958	T6959	nsubj	PAX6CTP,contains
R4343	T6960	T6961	det	the,peptide
R4344	T6961	T6959	dobj	peptide,contains
R4345	T6962	T6963	advmod	newly,defined
R4346	T6963	T6961	amod	defined,peptide
R4347	T6964	T6965	npadvmod	C,terminal
R4348	T6965	T6961	amod	terminal,peptide
R4349	T6966	T6965	punct	-,terminal
R435	T1023	T1021	cc	and,multiple
R4350	T6967	T6961	amod	alone,peptide
R4351	T6968	T6969	punct	(,391
R4352	T6969	T6959	parataxis	391,contains
R4353	T6970	T6971	punct	–,422
R4354	T6971	T6969	prep	422,391
R4355	T6972	T6969	punct	),391
R4356	T6973	T6959	punct	.,contains
R4357	T6975	T6976	nsubj	Primers,were
R4358	T6977	T6978	nmod	ST004,TT
R4359	T6978	T6976	attr	TT,were
R436	T1024	T1021	conj	diverse,multiple
R4360	T6979	T6980	punct	(,F
R4361	T6980	T6978	parataxis	F,TT
R4362	T6981	T6980	punct	),F
R4363	T6982	T6978	nummod	5,TT
R4364	T6983	T6982	punct	',5
R4365	T6984	T6978	punct	-,TT
R4366	T6985	T6978	compound	AAA,TT
R4367	T6986	T6978	compound	AGT,TT
R4368	T6987	T6978	compound	CGA,TT
R4369	T6988	T6978	compound	CTA,TT
R437	T1025	T1022	prep	for,roles
R4370	T6989	T6978	compound	CCA,TT
R4371	T6990	T6978	compound	CTT,TT
R4372	T6991	T6978	compound	CAA,TT
R4373	T6992	T6978	compound	CAG,TT
R4374	T6993	T6978	compound	GAC,TT
R4375	T6994	T6978	compound	TCA,TT
R4376	T6995	T6978	punct	-,TT
R4377	T6996	T6978	nummod	3,TT
R4378	T6997	T6978	punct	',TT
R4379	T6998	T6978	cc	and,TT
R438	T1026	T1025	pobj	PAX6,for
R4380	T6999	T6978	conj	ST005,TT
R4381	T7000	T7001	punct	(,R
R4382	T7001	T6999	parataxis	R,ST005
R4383	T7002	T7001	punct	),R
R4384	T7003	T6976	punct	.,were
R4385	T7005	T7006	compound	PAX6PST,CT
R4386	T7006	T7008	nsubj	CT,contains
R4387	T7007	T7006	punct	-,CT
R4388	T7009	T7010	det	the,domain
R4389	T7010	T7008	dobj	domain,contains
R439	T1027	T1022	prep	in,roles
R4390	T7011	T7010	compound	PST,domain
R4391	T7012	T7010	cc	minus,domain
R4392	T7013	T7014	det	the,peptide
R4393	T7014	T7010	conj	peptide,domain
R4394	T7015	T7016	npadvmod	C,terminal
R4395	T7016	T7014	amod	terminal,peptide
R4396	T7017	T7016	punct	-,terminal
R4397	T7018	T7019	punct	(,278
R4398	T7019	T7008	parataxis	278,contains
R4399	T7020	T7021	punct	–,390
R44	T227	T226	punct	-,of
R440	T1028	T1029	compound	brain,development
R4400	T7021	T7019	prep	390,278
R4401	T7022	T7019	punct	),278
R4402	T7023	T7008	punct	.,contains
R4403	T7025	T7026	nsubjpass	This,made
R4404	T7027	T7026	auxpass	was,made
R4405	T7028	T7026	prep	by,made
R4406	T7029	T7028	pcomp	cutting,by
R4407	T7030	T7029	dobj	PAX6PST,cutting
R4408	T7031	T7029	prep	with,cutting
R4409	T7032	T7031	pobj	NdeI,with
R441	T1029	T1027	pobj	development,in
R4410	T7033	T7032	cc	and,NdeI
R4411	T7034	T7032	conj	NotI,NdeI
R4412	T7035	T7036	aux	to,drop
R4413	T7036	T7029	advcl	drop,cutting
R4414	T7037	T7036	prt	out,drop
R4415	T7038	T7039	det	the,peptide
R4416	T7039	T7036	dobj	peptide,drop
R4417	T7040	T7041	npadvmod	C,terminal
R4418	T7041	T7039	amod	terminal,peptide
R4419	T7042	T7041	punct	-,terminal
R442	T1030	T1031	compound	MRI,analyses
R4420	T7043	T7029	punct	", ",cutting
R4421	T7044	T7029	cc	and,cutting
R4422	T7045	T7029	conj	inserting,cutting
R4423	T7046	T7047	det	a,linker
R4424	T7047	T7045	dobj	linker,inserting
R4425	T7048	T7047	amod	synthetic,linker
R4426	T7049	T7047	prep	between,linker
R4427	T7050	T7051	det	the,sites
R4428	T7051	T7049	pobj	sites,between
R4429	T7052	T7051	nummod	two,sites
R443	T1031	T1019	dobj	analyses,prompted
R4430	T7053	T7051	compound	restriction,sites
R4431	T7054	T7026	punct	.,made
R4432	T7056	T7057	det	All,fragments
R4433	T7057	T7058	nsubjpass	fragments,sequenced
R4434	T7059	T7057	acl	generated,fragments
R4435	T7060	T7059	prep	by,generated
R4436	T7061	T7060	pobj	PCR,by
R4437	T7062	T7060	cc	or,by
R4438	T7063	T7060	conj	with,by
R4439	T7064	T7063	pobj	linkers,with
R444	T1032	T1031	prep	of,analyses
R4440	T7065	T7058	auxpass	were,sequenced
R4441	T7066	T7067	aux	to,check
R4442	T7067	T7058	advcl	check,sequenced
R4443	T7068	T7069	mark	that,introduced
R4444	T7069	T7067	ccomp	introduced,check
R4445	T7070	T7071	det	no,errors
R4446	T7071	T7069	nsubjpass	errors,introduced
R4447	T7072	T7069	aux	had,introduced
R4448	T7073	T7069	auxpass	been,introduced
R4449	T7074	T7058	punct	.,sequenced
R445	T1033	T1034	compound	aniridia,patients
R4450	T7076	T7077	det	A,construct
R4451	T7077	T7081	nsubjpass	construct,generated
R4452	T7078	T7079	compound	PAX6,domain
R4453	T7079	T7077	compound	domain,construct
R4454	T7080	T7079	compound	PST,domain
R4455	T7082	T7077	acl	containing,construct
R4456	T7083	T7084	det	the,mutation
R4457	T7084	T7082	dobj	mutation,containing
R4458	T7085	T7084	appos	1627A,mutation
R4459	T7086	T7087	punct	>,G
R446	T1034	T1032	pobj	patients,of
R4460	T7087	T7085	prep	G,1627A
R4461	T7088	T7084	punct	(,mutation
R4462	T7089	T7084	appos	Q422R,mutation
R4463	T7090	T7084	punct	),mutation
R4464	T7091	T7081	auxpass	was,generated
R4465	T7092	T7081	prep	in,generated
R4466	T7093	T7094	det	the,way
R4467	T7094	T7092	pobj	way,in
R4468	T7095	T7094	amod	same,way
R4469	T7096	T7094	prep	as,way
R447	T1035	T1019	punct	", ",prompted
R4470	T7097	T7098	det	the,construct
R4471	T7098	T7096	pobj	construct,as
R4472	T7099	T7098	compound	PAX6PST,construct
R4473	T7100	T7092	punct	", ",in
R4474	T7101	T7092	cc	but,in
R4475	T7102	T7092	conj	using,in
R4476	T7103	T7104	det	the,primer
R4477	T7104	T7102	dobj	primer,using
R4478	T7105	T7104	amod	reverse,primer
R4479	T7106	T7104	compound	PCR,primer
R448	T1036	T1019	cc	and,prompted
R4480	T7107	T7108	nmod	ST006,AG
R4481	T7108	T7104	appos	AG,primer
R4482	T7109	T7108	nummod	5,AG
R4483	T7110	T7109	punct	',5
R4484	T7111	T7108	punct	-,AG
R4485	T7112	T7108	compound	TTT,AG
R4486	T7113	T7108	compound	TGC,AG
R4487	T7114	T7108	compound	GGC,AG
R4488	T7115	T7108	compound	CGC,AG
R4489	T7116	T7108	compound	TTT,AG
R449	T1037	T1038	det	a,range
R4490	T7117	T7108	compound	TTA,AG
R4491	T7118	T7108	compound	CCG,AG
R4492	T7119	T7108	compound	TAA,AG
R4493	T7120	T7108	compound	TCT,AG
R4494	T7121	T7108	compound	TGG,AG
R4495	T7122	T7108	compound	CCA,AG
R4496	T7123	T7108	compound	GTA,AG
R4497	T7124	T7108	compound	TTG,AG
R4498	T7125	T7108	punct	-,AG
R4499	T7126	T7108	nummod	3,AG
R45	T228	T226	pobj	function,of
R450	T1038	T1039	nsubjpass	range,uncovered
R4500	T7127	T7108	punct	',AG
R4501	T7128	T7104	punct	", ",primer
R4502	T7129	T7130	dep	which,contains
R4503	T7130	T7104	relcl	contains,primer
R4504	T7131	T7132	det	the,substitution
R4505	T7132	T7130	dobj	substitution,contains
R4506	T7133	T7132	compound	mutant,substitution
R4507	T7134	T7132	compound	nucleotide,substitution
R4508	T7135	T7136	punct	(,underlined
R4509	T7136	T7104	parataxis	underlined,primer
R451	T1039	T1019	conj	uncovered,prompted
R4510	T7137	T7136	punct	),underlined
R4511	T7138	T7081	punct	.,generated
R4512	T7140	T7141	compound	cDNA,sequences
R4513	T7141	T7142	nsubjpass	sequences,generated
R4514	T7143	T7141	acl	containing,sequences
R4515	T7144	T7145	det	the,mutations
R4516	T7145	T7143	dobj	mutations,containing
R4517	T7146	T7145	appos	1615del10,mutations
R4518	T7147	T7148	punct	[,28
R4519	T7148	T7146	parataxis	28,1615del10
R452	T1040	T1038	prep	of,range
R4520	T7149	T7148	punct	],28
R4521	T7150	T7146	cc	and,1615del10
R4522	T7151	T7146	conj	1629insT,1615del10
R4523	T7152	T7151	punct	(,1629insT
R4524	T7153	T7151	appos	X423L,1629insT
R4525	T7154	T7151	punct	),1629insT
R4526	T7155	T7156	punct	[,12
R4527	T7156	T7151	parataxis	12,1629insT
R4528	T7157	T7156	punct	],12
R4529	T7158	T7142	auxpass	were,generated
R453	T1041	T1042	amod	distinctive,anomalies
R4530	T7159	T7142	prep	by,generated
R4531	T7160	T7159	pobj	PCR,by
R4532	T7161	T7160	prep	from,PCR
R4533	T7162	T7163	amod	reverse,transcribed
R4534	T7163	T7164	amod	transcribed,RNA
R4535	T7164	T7161	pobj	RNA,from
R4536	T7165	T7166	punct	(,gift
R4537	T7166	T7164	parataxis	gift,RNA
R4538	T7167	T7166	det	a,gift
R4539	T7168	T7166	prep	from,gift
R454	T1042	T1040	pobj	anomalies,of
R4540	T7169	T7170	compound	Dr,Williamson
R4541	T7170	T7168	pobj	Williamson,from
R4542	T7171	T7170	compound	K,Williamson
R4543	T7172	T7170	cc	and,Williamson
R4544	T7173	T7174	compound	Prof,Heyningen
R4545	T7174	T7170	conj	Heyningen,Williamson
R4546	T7175	T7174	compound	V,Heyningen
R4547	T7176	T7174	compound	van,Heyningen
R4548	T7177	T7166	punct	),gift
R4549	T7178	T7142	punct	.,generated
R455	T1043	T1042	compound	brain,anomalies
R4550	T7180	T7181	nsubj	Primers,were
R4551	T7182	T7183	nmod	ST015,C
R4552	T7183	T7181	attr	C,were
R4553	T7184	T7185	punct	(,F
R4554	T7185	T7183	parataxis	F,C
R4555	T7186	T7185	punct	),F
R4556	T7187	T7183	nummod	5,C
R4557	T7188	T7187	punct	',5
R4558	T7189	T7183	punct	-,C
R4559	T7190	T7183	compound	CCC,C
R456	T1044	T1039	auxpass	were,uncovered
R4560	T7191	T7183	compound	ACA,C
R4561	T7192	T7183	compound	TAT,C
R4562	T7193	T7183	compound	GCA,C
R4563	T7194	T7183	compound	GAC,C
R4564	T7195	T7183	compound	ACA,C
R4565	T7196	T7183	punct	-,C
R4566	T7197	T7183	nummod	3,C
R4567	T7198	T7183	punct	',C
R4568	T7199	T7183	cc	and,C
R4569	T7200	T7201	nmod	ST031,TC
R457	T1045	T1039	punct	.,uncovered
R4570	T7201	T7183	conj	TC,C
R4571	T7202	T7203	punct	(,R
R4572	T7203	T7201	parataxis	R,TC
R4573	T7204	T7203	punct	),R
R4574	T7205	T7201	nummod	5,TC
R4575	T7206	T7205	punct	',5
R4576	T7207	T7201	punct	-,TC
R4577	T7208	T7201	compound	TTG,TC
R4578	T7209	T7201	compound	CGG,TC
R4579	T7210	T7201	compound	CCG,TC
R458	T1047	T1048	det	The,common
R4580	T7211	T7201	compound	CAT,TC
R4581	T7212	T7201	compound	CCA,TC
R4582	T7213	T7201	compound	TCC,TC
R4583	T7214	T7201	compound	AGT,TC
R4584	T7215	T7201	compound	CTA,TC
R4585	T7216	T7201	compound	CAT,TC
R4586	T7217	T7201	compound	TGT,TC
R4587	T7218	T7201	punct	-,TC
R4588	T7219	T7201	nummod	3,TC
R4589	T7220	T7201	punct	',TC
R459	T1048	T1050	nsubj	common,was
R4590	T7221	T7181	punct	.,were
R4591	T7223	T7224	det	The,construct
R4592	T7224	T7226	nsubjpass	construct,cut
R4593	T7225	T7224	compound	PAX6PST,construct
R4594	T7227	T7226	auxpass	was,cut
R4595	T7228	T7226	prep	with,cut
R4596	T7229	T7228	pobj	NdeI,with
R4597	T7230	T7229	cc	and,NdeI
R4598	T7231	T7229	conj	NotI,NdeI
R4599	T7232	T7233	aux	to,release
R46	T229	T219	prep	in,Individuals
R460	T1049	T1048	advmod	most,common
R4600	T7233	T7226	advcl	release,cut
R4601	T7234	T7235	det	the,peptide
R4602	T7235	T7233	dobj	peptide,release
R4603	T7236	T7235	amod	normal,peptide
R4604	T7237	T7238	npadvmod	C,terminal
R4605	T7238	T7235	amod	terminal,peptide
R4606	T7239	T7238	punct	-,terminal
R4607	T7240	T7226	punct	", ",cut
R4608	T7241	T7226	cc	and,cut
R4609	T7242	T7243	det	the,sequence
R461	T1051	T1048	cc	and,common
R4610	T7243	T7245	nsubjpass	sequence,inserted
R4611	T7244	T7243	compound	mutant,sequence
R4612	T7245	T7226	conj	inserted,cut
R4613	T7246	T7245	auxpass	was,inserted
R4614	T7247	T7245	punct	.,inserted
R4615	T7368	T7369	nmod	Yeast,screens
R4616	T7370	T7371	nummod	two,hybrid
R4617	T7371	T7369	compound	hybrid,screens
R4618	T7372	T7371	punct	-,hybrid
R4619	T7373	T7369	compound	library,screens
R462	T1052	T1048	conj	striking,common
R4620	T7375	T7376	det	A,library
R4621	T7376	T7380	nsubjpass	library,screened
R4622	T7377	T7378	compound	mouse,brain
R4623	T7378	T7376	compound	brain,library
R4624	T7379	T7376	compound	cDNA,library
R4625	T7381	T7382	punct	(,ProQuest
R4626	T7382	T7376	parataxis	ProQuest,library
R4627	T7383	T7382	punct	", ",ProQuest
R4628	T7384	T7382	npadvmod	Invitrogen,ProQuest
R4629	T7385	T7382	punct	),ProQuest
R463	T1053	T1048	prep	of,common
R4630	T7386	T7380	auxpass	was,screened
R4631	T7387	T7380	prep	with,screened
R4632	T7388	T7389	det	the,constructs
R4633	T7389	T7387	pobj	constructs,with
R4634	T7390	T7389	nmod	PAX6PST,constructs
R4635	T7391	T7390	cc	and,PAX6PST
R4636	T7392	T7390	conj	PAX6CTP,PAX6PST
R4637	T7393	T7389	compound	pDBLeu,constructs
R4638	T7394	T7380	punct	.,screened
R4639	T7396	T7397	det	The,library
R464	T1054	T1053	pobj	these,of
R4640	T7397	T7398	nsubjpass	library,constructed
R4641	T7399	T7398	auxpass	was,constructed
R4642	T7400	T7398	prep	in,constructed
R4643	T7401	T7402	det	the,vector
R4644	T7402	T7400	pobj	vector,in
R4645	T7403	T7402	compound	pPC86,vector
R4646	T7404	T7402	punct	", ",vector
R4647	T7405	T7406	dep	which,produces
R4648	T7406	T7402	relcl	produces,vector
R4649	T7407	T7406	dobj	proteins,produces
R465	T1055	T1050	attr	absence,was
R4650	T7408	T7407	acl	fused,proteins
R4651	T7409	T7408	prep	to,fused
R4652	T7410	T7411	det	the,domain
R4653	T7411	T7409	pobj	domain,to
R4654	T7412	T7411	compound	yeast,domain
R4655	T7413	T7411	compound	GAL4,domain
R4656	T7414	T7411	compound	activation,domain
R4657	T7415	T7398	punct	.,constructed
R4658	T7417	T7418	det	The,system
R4659	T7418	T7419	nsubj	system,uses
R466	T1056	T1055	cc	or,absence
R4660	T7420	T7421	nummod	three,genes
R4661	T7421	T7419	dobj	genes,uses
R4662	T7422	T7423	npadvmod	GAL4,activated
R4663	T7423	T7421	amod	activated,genes
R4664	T7424	T7423	punct	-,activated
R4665	T7425	T7421	compound	reporter,genes
R4666	T7426	T7421	punct	", ",genes
R4667	T7427	T7421	appos	HIS3,genes
R4668	T7428	T7427	punct	", ",HIS3
R4669	T7429	T7427	conj	URA3,HIS3
R467	T1057	T1055	conj	hypoplasia,absence
R4670	T7430	T7429	cc	and,URA3
R4671	T7431	T7429	conj	lacZ,URA3
R4672	T7432	T7419	punct	", ",uses
R4673	T7433	T7434	aux	to,identify
R4674	T7434	T7419	advcl	identify,uses
R4675	T7435	T7436	amod	positive,interactions
R4676	T7436	T7434	dobj	interactions,identify
R4677	T7437	T7419	punct	.,uses
R4678	T7439	T7440	nsubjpass	Reporters,activated
R4679	T7441	T7440	auxpass	are,activated
R468	T1058	T1055	prep	of,absence
R4680	T7442	T7443	advmod	when,interacts
R4681	T7443	T7440	advcl	interacts,activated
R4682	T7444	T7445	det	the,protein
R4683	T7445	T7443	nsubj	protein,interacts
R4684	T7446	T7445	compound	bait,protein
R4685	T7447	T7445	acl	fused,protein
R4686	T7448	T7447	prep	to,fused
R4687	T7449	T7450	det	the,domain
R4688	T7450	T7448	pobj	domain,to
R4689	T7451	T7450	compound	GAL4,domain
R469	T1059	T1060	det	the,commissure
R4690	T7452	T7450	compound	DNA,domain
R4691	T7453	T7450	compound	binding,domain
R4692	T7454	T7450	punct	(,domain
R4693	T7455	T7450	appos	pDBLeu,domain
R4694	T7456	T7450	punct	),domain
R4695	T7457	T7443	prep	with,interacts
R4696	T7458	T7459	det	the,protein
R4697	T7459	T7457	pobj	protein,with
R4698	T7460	T7459	compound	prey,protein
R4699	T7461	T7459	acl	fused,protein
R47	T230	T231	det	the,gene
R470	T1060	T1058	pobj	commissure,of
R4700	T7462	T7461	prep	to,fused
R4701	T7463	T7464	det	the,domain
R4702	T7464	T7462	pobj	domain,to
R4703	T7465	T7464	compound	GAL4,domain
R4704	T7466	T7464	compound	activation,domain
R4705	T7467	T7464	punct	(,domain
R4706	T7468	T7464	appos	pPC86,domain
R4707	T7469	T7464	punct	),domain
R4708	T7470	T7440	punct	", ",activated
R4709	T7471	T7472	advmod	thus,reconstituting
R471	T1061	T1060	amod	anterior,commissure
R4710	T7472	T7440	advcl	reconstituting,activated
R4711	T7473	T7474	compound	GAL4,function
R4712	T7474	T7472	dobj	function,reconstituting
R4713	T7475	T7440	punct	.,activated
R4714	T7477	T7478	compound	HIS3,activation
R4715	T7478	T7479	nsubj	activation,allows
R4716	T7480	T7479	dobj	growth,allows
R4717	T7481	T7479	prep	on,allows
R4718	T7482	T7481	pobj	plates,on
R4719	T7483	T7482	acl	lacking,plates
R472	T1062	T1063	punct	[,11
R4720	T7484	T7483	dobj	histidine,lacking
R4721	T7485	T7479	punct	.,allows
R4722	T7487	T7488	amod	Weak,activation
R4723	T7488	T7490	nsubj	activation,suppresses
R4724	T7489	T7488	compound	URA3,activation
R4725	T7491	T7490	dobj	growth,suppresses
R4726	T7492	T7490	prep	on,suppresses
R4727	T7493	T7492	pobj	plates,on
R4728	T7494	T7493	acl	containing,plates
R4729	T7495	T7496	advmod	5,orotic
R473	T1063	T1050	parataxis	11,was
R4730	T7496	T7500	amod	orotic,acid
R4731	T7497	T7496	punct	-,orotic
R4732	T7498	T7496	amod	fluro,orotic
R4733	T7499	T7496	punct	-,orotic
R4734	T7500	T7494	dobj	acid,containing
R4735	T7501	T7502	mark	while,permits
R4736	T7502	T7490	advcl	permits,suppresses
R4737	T7503	T7504	amod	strong,activation
R4738	T7504	T7502	nsubj	activation,permits
R4739	T7505	T7504	compound	URA3,activation
R474	T1064	T1063	punct	],11
R4740	T7506	T7502	dobj	growth,permits
R4741	T7507	T7502	prep	on,permits
R4742	T7508	T7507	pobj	plates,on
R4743	T7509	T7508	acl	lacking,plates
R4744	T7510	T7509	dobj	uracil,lacking
R4745	T7511	T7490	punct	.,suppresses
R4746	T7513	T7514	compound	LacZ,activation
R4747	T7514	T7515	nsubj	activation,causes
R4748	T7516	T7517	compound	X,gal
R4749	T7517	T7519	nsubj	gal,turn
R475	T1065	T1050	punct	.,was
R4750	T7518	T7517	punct	-,gal
R4751	T7519	T7515	ccomp	turn,causes
R4752	T7520	T7519	aux	to,turn
R4753	T7521	T7519	oprd	blue,turn
R4754	T7522	T7521	prep	in,blue
R4755	T7523	T7524	det	a,assay
R4756	T7524	T7522	pobj	assay,in
R4757	T7525	T7526	compound	beta,galactosidase
R4758	T7526	T7524	compound	galactosidase,assay
R4759	T7527	T7526	punct	-,galactosidase
R476	T1067	T1068	amod	Other,defects
R4760	T7528	T7515	punct	.,causes
R4761	T7530	T7531	det	All,assays
R4762	T7531	T7532	nsubjpass	assays,carried
R4763	T7533	T7532	auxpass	were,carried
R4764	T7534	T7532	prt	out,carried
R4765	T7535	T7532	prep	in,carried
R4766	T7536	T7535	pobj	parallel,in
R4767	T7537	T7536	prep	with,parallel
R4768	T7538	T7539	det	the,strains
R4769	T7539	T7537	pobj	strains,with
R477	T1068	T1069	nsubj	defects,included
R4770	T7540	T7539	nummod	five,strains
R4771	T7541	T7539	compound	ProQuest,strains
R4772	T7542	T7539	compound	control,strains
R4773	T7543	T7539	compound	yeast,strains
R4774	T7544	T7539	appos	A,strains
R4775	T7545	T7546	punct	-,E
R4776	T7546	T7544	prep	E,A
R4777	T7547	T7539	punct	", ",strains
R4778	T7548	T7549	dep	which,range
R4779	T7549	T7539	relcl	range,strains
R478	T1070	T1069	dobj	absence,included
R4780	T7550	T7549	prep	from,range
R4781	T7551	T7550	pobj	non-interactor,from
R4782	T7552	T7553	punct	(,A
R4783	T7553	T7551	parataxis	A,non-interactor
R4784	T7554	T7553	punct	),A
R4785	T7555	T7550	prep	to,from
R4786	T7556	T7557	amod	strong,interactor
R4787	T7557	T7555	pobj	interactor,to
R4788	T7558	T7559	punct	(,E
R4789	T7559	T7557	parataxis	E,interactor
R479	T1071	T1070	cc	or,absence
R4790	T7560	T7559	punct	),E
R4791	T7561	T7532	punct	.,carried
R4792	T7563	T7564	det	All,procedures
R4793	T7564	T7565	nsubjpass	procedures,carried
R4794	T7566	T7565	auxpass	were,carried
R4795	T7567	T7565	prt	out,carried
R4796	T7568	T7565	prep	according,carried
R4797	T7569	T7568	prep	to,according
R4798	T7570	T7571	det	the,supplier
R4799	T7571	T7572	poss	supplier,protocols
R48	T231	T229	pobj	gene,in
R480	T1072	T1070	conj	hypoplasia,absence
R4800	T7572	T7569	pobj	protocols,to
R4801	T7573	T7571	case	's,supplier
R4802	T7574	T7565	punct	.,carried
R4803	T7576	T7577	advmod	Briefly,co-transformed
R4804	T7578	T7577	punct	", ",co-transformed
R4805	T7579	T7580	advmod	chemically,competent
R4806	T7580	T7581	amod	competent,cells
R4807	T7581	T7577	nsubjpass	cells,co-transformed
R4808	T7582	T7581	compound	MaV203,cells
R4809	T7583	T7581	compound	yeast,cells
R481	T1073	T1070	prep	of,absence
R4810	T7584	T7577	auxpass	were,co-transformed
R4811	T7585	T7577	prep	with,co-transformed
R4812	T7586	T7587	det	the,library
R4813	T7587	T7585	pobj	library,with
R4814	T7588	T7587	compound	cDNA,library
R4815	T7589	T7587	cc	and,library
R4816	T7590	T7591	det	the,plasmid
R4817	T7591	T7587	conj	plasmid,library
R4818	T7592	T7591	compound	bait,plasmid
R4819	T7593	T7594	compound	pDBLeu,PAX6CTP
R482	T1074	T1075	det	the,gland
R4820	T7594	T7591	appos	PAX6CTP,plasmid
R4821	T7595	T7594	cc	or,PAX6CTP
R4822	T7596	T7597	compound	pDBLeu,PAX6PST
R4823	T7597	T7594	conj	PAX6PST,PAX6CTP
R4824	T7598	T7577	punct	.,co-transformed
R4825	T7600	T7601	nsubjpass	Transformants,plated
R4826	T7602	T7603	punct	(,107
R4827	T7603	T7600	parataxis	107,Transformants
R4828	T7604	T7603	nummod	5,107
R4829	T7605	T7603	punct	×,107
R483	T1075	T1073	pobj	gland,of
R4830	T7606	T7603	punct	),107
R4831	T7607	T7601	auxpass	were,plated
R4832	T7608	T7601	prep	on,plated
R4833	T7609	T7608	pobj	medium,on
R4834	T7610	T7609	acl	lacking,medium
R4835	T7611	T7610	dobj	histidine,lacking
R4836	T7612	T7613	aux	to,check
R4837	T7613	T7601	advcl	check,plated
R4838	T7614	T7613	prep	for,check
R4839	T7615	T7616	compound	HIS3,activation
R484	T1076	T1075	amod	pineal,gland
R4840	T7616	T7614	pobj	activation,for
R4841	T7617	T7601	punct	.,plated
R4842	T7619	T7620	compound	HIS3,positives
R4843	T7620	T7621	nsubjpass	positives,assayed
R4844	T7622	T7621	auxpass	were,assayed
R4845	T7623	T7621	advmod	then,assayed
R4846	T7624	T7621	prep	for,assayed
R4847	T7625	T7626	det	all,reporters
R4848	T7626	T7624	pobj	reporters,for
R4849	T7627	T7626	nummod	3,reporters
R485	T1077	T1070	punct	", ",absence
R4850	T7628	T7621	punct	.,assayed
R4851	T7630	T7631	det	The,plasmid
R4852	T7631	T7634	nsubjpass	plasmid,isolated
R4853	T7632	T7631	compound	pPC86,plasmid
R4854	T7633	T7631	compound	prey,plasmid
R4855	T7635	T7634	auxpass	was,isolated
R4856	T7636	T7634	prep	from,isolated
R4857	T7637	T7638	det	all,positives
R4858	T7638	T7636	pobj	positives,from
R4859	T7639	T7640	compound	HIS3,LacZ
R486	T1078	T1079	amod	cortical,polymicrogyria
R4860	T7640	T7638	compound	LacZ,positives
R4861	T7641	T7640	punct	/,LacZ
R4862	T7642	T7640	compound	URA3,LacZ
R4863	T7643	T7640	punct	/,LacZ
R4864	T7644	T7634	cc	and,isolated
R4865	T7645	T7646	det	the,insert
R4866	T7646	T7648	nsubj	insert,sequenced
R4867	T7647	T7646	compound	cDNA,insert
R4868	T7648	T7634	conj	sequenced,isolated
R4869	T7649	T7648	punct	.,sequenced
R487	T1079	T1070	conj	polymicrogyria,absence
R4870	T7651	T7652	compound	BLAST,searches
R4871	T7652	T7653	nsubjpass	searches,performed
R4872	T7654	T7653	auxpass	were,performed
R4873	T7655	T7656	aux	to,identify
R4874	T7656	T7653	advcl	identify,performed
R4875	T7657	T7658	det	the,insert
R4876	T7658	T7656	dobj	insert,identify
R4877	T7659	T7658	compound	cDNA,insert
R4878	T7660	T7661	punct	[,24
R4879	T7661	T7653	parataxis	24,performed
R488	T1080	T1079	punct	", ",polymicrogyria
R4880	T7662	T7661	punct	],24
R4881	T7663	T7653	punct	.,performed
R4882	T7711	T7712	amod	Pairwise,interactions
R4883	T7714	T7715	amod	Specific,interactions
R4884	T7715	T7716	nsubjpass	interactions,tested
R4885	T7717	T7716	auxpass	were,tested
R4886	T7718	T7716	prep	by,tested
R4887	T7719	T7718	pcomp	transforming,by
R4888	T7720	T7721	amod	competent,cells
R4889	T7721	T7719	dobj	cells,transforming
R489	T1081	T1082	amod	white,matter
R4890	T7722	T7721	compound	MaV203,cells
R4891	T7723	T7721	compound	yeast,cells
R4892	T7724	T7721	prep	with,cells
R4893	T7725	T7726	nummod	one,bait
R4894	T7726	T7727	compound	bait,construct
R4895	T7727	T7724	pobj	construct,with
R4896	T7728	T7727	punct	(,construct
R4897	T7729	T7727	prep	in,construct
R4898	T7730	T7729	pobj	pDBLeu,in
R4899	T7731	T7727	punct	),construct
R49	T232	T231	compound	PAX6,gene
R490	T1082	T1083	compound	matter,changes
R4900	T7732	T7727	cc	and,construct
R4901	T7733	T7734	nummod	one,prey
R4902	T7734	T7735	compound	prey,construct
R4903	T7735	T7727	conj	construct,construct
R4904	T7736	T7735	punct	(,construct
R4905	T7737	T7735	prep	in,construct
R4906	T7738	T7737	pobj	pPC86,in
R4907	T7739	T7719	punct	),transforming
R4908	T7740	T7719	cc	and,transforming
R4909	T7741	T7719	conj	testing,transforming
R491	T1083	T1079	conj	changes,polymicrogyria
R4910	T7742	T7743	det	the,colonies
R4911	T7743	T7741	dobj	colonies,testing
R4912	T7744	T7743	amod	resulting,colonies
R4913	T7745	T7741	prep	for,testing
R4914	T7746	T7745	pobj	activation,for
R4915	T7747	T7746	prep	of,activation
R4916	T7748	T7749	det	the,reporters
R4917	T7749	T7747	pobj	reporters,of
R4918	T7750	T7749	nmod	HIS3,reporters
R4919	T7751	T7750	punct	", ",HIS3
R492	T1084	T1083	prep	in,changes
R4920	T7752	T7750	conj	URA3,HIS3
R4921	T7753	T7752	cc	and,URA3
R4922	T7754	T7752	conj	LacZ,URA3
R4923	T7755	T7741	prep	as,testing
R4924	T7756	T7755	pcomp	before,as
R4925	T7757	T7716	punct	.,tested
R4926	T7759	T7760	aux	To,create
R4927	T7760	T7761	advcl	create,identified
R4928	T7762	T7763	det	a,clone
R4929	T7763	T7760	dobj	clone,create
R493	T1085	T1086	det	the,callosum
R4930	T7764	T7765	amod	full,length
R4931	T7765	T7763	compound	length,clone
R4932	T7766	T7765	punct	-,length
R4933	T7767	T7763	compound	Homer3,clone
R4934	T7768	T7760	prep	for,create
R4935	T7769	T7770	amod	pairwise,tests
R4936	T7770	T7768	pobj	tests,for
R4937	T7771	T7761	punct	", ",identified
R4938	T7772	T7773	det	a,clone
R4939	T7773	T7761	nsubjpass	clone,identified
R494	T1086	T1084	pobj	callosum,in
R4940	T7774	T7773	compound	cDNA,clone
R4941	T7775	T7776	punct	(,clone
R4942	T7776	T7773	parataxis	clone,clone
R4943	T7777	T7776	compound	IMAGE,clone
R4944	T7778	T7776	nummod	3602414,clone
R4945	T7779	T7776	punct	", ",clone
R4946	T7780	T7781	compound	accession,BE569374
R4947	T7781	T7776	appos	BE569374,clone
R4948	T7782	T7781	compound	number,BE569374
R4949	T7783	T7776	punct	),clone
R495	T1087	T1086	compound	corpus,callosum
R4950	T7784	T7773	acl	containing,clone
R4951	T7785	T7786	det	the,acids
R4952	T7786	T7784	dobj	acids,containing
R4953	T7787	T7786	amod	missing,acids
R4954	T7788	T7789	npadvmod	N,terminal
R4955	T7789	T7786	amod	terminal,acids
R4956	T7790	T7789	punct	-,terminal
R4957	T7791	T7786	nummod	70,acids
R4958	T7792	T7786	compound	amino,acids
R4959	T7793	T7761	auxpass	was,identified
R496	T1088	T1083	cc	and,changes
R4960	T7794	T7761	prep	by,identified
R4961	T7795	T7796	det	a,search
R4962	T7796	T7794	pobj	search,by
R4963	T7797	T7796	compound	BLAST,search
R4964	T7798	T7796	prep	of,search
R4965	T7799	T7800	det	the,database
R4966	T7800	T7798	pobj	database,of
R4967	T7801	T7800	compound	EST,database
R4968	T7802	T7803	compound	nucleotide,sequence
R4969	T7803	T7800	compound	sequence,database
R497	T1089	T1090	amod	grey,matter
R4970	T7804	T7761	cc	and,identified
R4971	T7805	T7761	conj	obtained,identified
R4972	T7806	T7805	prep	from,obtained
R4973	T7807	T7808	det	the,Centre
R4974	T7808	T7806	pobj	Centre,from
R4975	T7809	T7810	compound	Rosalind,Franklin
R4976	T7810	T7808	compound	Franklin,Centre
R4977	T7811	T7808	prep	for,Centre
R4978	T7812	T7813	compound	Genomics,Research
R4979	T7813	T7811	pobj	Research,for
R498	T1090	T1091	compound	matter,changes
R4980	T7814	T7761	punct	.,identified
R4981	T7816	T7817	det	The,fragment
R4982	T7817	T7819	nsubj	fragment,amplified
R4983	T7818	T7817	amod	missing,fragment
R4984	T7820	T7819	aux	was,amplified
R4985	T7821	T7819	prep	from,amplified
R4986	T7822	T7823	det	the,clone
R4987	T7823	T7821	pobj	clone,from
R4988	T7824	T7823	compound	IMAGE,clone
R4989	T7825	T7819	prep	by,amplified
R499	T1091	T1083	conj	changes,changes
R4990	T7826	T7825	pobj	PCR,by
R4991	T7827	T7819	cc	and,amplified
R4992	T7828	T7819	conj	inserted,amplified
R4993	T7829	T7828	prep	into,inserted
R4994	T7830	T7831	det	the,plasmid
R4995	T7831	T7829	pobj	plasmid,into
R4996	T7832	T7833	compound	pPC86,Homer3
R4997	T7833	T7831	compound	Homer3,plasmid
R4998	T7834	T7833	punct	-,Homer3
R4999	T7835	T7836	aux	to,create
R5	T181	T182	dep	that,interact
R50	T233	T220	dobj	malformations,have
R500	T1092	T1091	prep	in,changes
R5000	T7836	T7819	advcl	create,amplified
R5001	T7837	T7838	det	a,construct
R5002	T7838	T7836	dobj	construct,create
R5003	T7839	T7840	amod	full,length
R5004	T7840	T7838	compound	length,construct
R5005	T7841	T7840	punct	-,length
R5006	T7842	T7838	compound	expression,construct
R5007	T7843	T7819	punct	.,amplified
R5008	T7962	T7963	amod	Semi-quantitative,PCR
R5009	T7965	T7966	aux	To,check
R501	T1093	T1094	det	the,cerebellum
R5010	T7966	T7967	advcl	check,performed
R5011	T7968	T7969	det	the,representation
R5012	T7969	T7966	dobj	representation,check
R5013	T7970	T7969	amod	relative,representation
R5014	T7971	T7969	prep	of,representation
R5015	T7972	T7971	pobj	clones,of
R5016	T7973	T7969	prep	in,representation
R5017	T7974	T7975	det	the,library
R5018	T7975	T7973	pobj	library,in
R5019	T7976	T7975	compound	cDNA,library
R502	T1094	T1092	pobj	cerebellum,in
R5020	T7977	T7967	punct	", ",performed
R5021	T7978	T7979	amod	semi-quantitative,PCR
R5022	T7979	T7967	nsubjpass	PCR,performed
R5023	T7980	T7967	auxpass	was,performed
R5024	T7981	T7967	prep	on,performed
R5025	T7982	T7981	pobj	Pax6,on
R5026	T7983	T7982	punct	", ",Pax6
R5027	T7984	T7982	conj	Homer3,Pax6
R5028	T7985	T7984	punct	", ",Homer3
R5029	T7986	T7984	conj	Dncl1,Homer3
R503	T1095	T1096	punct	[,11
R5030	T7987	T7986	punct	", ",Dncl1
R5031	T7988	T7986	conj	Trim11,Dncl1
R5032	T7989	T7988	cc	and,Trim11
R5033	T7990	T7991	det	the,genes
R5034	T7991	T7988	conj	genes,Trim11
R5035	T7992	T7993	advmod	constitutively,expressed
R5036	T7993	T7991	amod	expressed,genes
R5037	T7994	T7991	appos	Gapdh,genes
R5038	T7995	T7994	cc	and,Gapdh
R5039	T7996	T7994	conj	Atp5a1,Gapdh
R504	T1096	T1069	parataxis	11,included
R5040	T7997	T7998	punct	[,30
R5041	T7998	T7967	parataxis	30,performed
R5042	T7999	T7998	nummod	29,30
R5043	T8000	T7998	punct	",",30
R5044	T8001	T7998	punct	],30
R5045	T8002	T7967	punct	.,performed
R5046	T8004	T8005	nsubjpass	Primers,designed
R5047	T8006	T8005	auxpass	were,designed
R5048	T8007	T8008	aux	to,cross
R5049	T8008	T8005	advcl	cross,designed
R505	T1097	T1098	punct	-,13
R5050	T8009	T8010	advmod	at,one
R5051	T8010	T8012	nummod	one,intron
R5052	T8011	T8010	advmod	least,one
R5053	T8012	T8008	dobj	intron,cross
R5054	T8013	T8005	punct	", ",designed
R5055	T8014	T8015	mark	so,amplified
R5056	T8015	T8005	advcl	amplified,designed
R5057	T8016	T8015	mark	that,amplified
R5058	T8017	T8018	advmod	only,clones
R5059	T8018	T8015	nsubjpass	clones,amplified
R506	T1098	T1096	prep	13,11
R5060	T8019	T8020	advmod	correctly,spliced
R5061	T8020	T8018	amod	spliced,clones
R5062	T8021	T8015	auxpass	were,amplified
R5063	T8022	T8005	punct	.,designed
R5064	T8024	T8025	compound	Primer,sequences
R5065	T8025	T8026	nsubj	sequences,were
R5066	T8027	T8026	punct	: ,were
R5067	T8028	T8026	attr	Pax6,were
R5068	T8029	T8028	punct	-,Pax6
R5069	T8030	T8031	compound	F,CTG
R507	T1099	T1096	punct	],11
R5070	T8031	T8028	appos	CTG,Pax6
R5071	T8032	T8031	compound	CAG,CTG
R5072	T8033	T8031	compound	CCA,CTG
R5073	T8034	T8031	compound	AAA,CTG
R5074	T8035	T8031	compound	TAG,CTG
R5075	T8036	T8031	compound	ATC,CTG
R5076	T8037	T8031	compound	TAC,CTG
R5077	T8038	T8028	punct	;,Pax6
R5078	T8039	T8028	appos	Pax6,Pax6
R5079	T8040	T8039	punct	-,Pax6
R508	T1100	T1069	punct	.,included
R5080	T8041	T8042	compound	R,TT
R5081	T8042	T8039	appos	TT,Pax6
R5082	T8043	T8042	compound	CGA,TT
R5083	T8044	T8042	compound	TCA,TT
R5084	T8045	T8042	compound	CAT,TT
R5085	T8046	T8042	compound	GCT,TT
R5086	T8047	T8042	compound	CTC,TT
R5087	T8048	T8042	compound	TCC,TT
R5088	T8049	T8028	punct	;,Pax6
R5089	T8050	T8028	appos	Homer3,Pax6
R509	T1102	T1103	amod	Functional,changes
R5090	T8051	T8050	punct	-,Homer3
R5091	T8052	T8053	compound	F,AGC
R5092	T8053	T8050	appos	AGC,Homer3
R5093	T8054	T8053	compound	CCC,AGC
R5094	T8055	T8053	compound	AGG,AGC
R5095	T8056	T8053	compound	TGG,AGC
R5096	T8057	T8053	compound	CTG,AGC
R5097	T8058	T8053	compound	TAG,AGC
R5098	T8059	T8028	punct	;,Pax6
R5099	T8060	T8028	appos	Homer3,Pax6
R51	T234	T233	prep	of,malformations
R510	T1103	T1104	nsubj	changes,included
R5100	T8061	T8060	punct	-,Homer3
R5101	T8062	T8063	compound	R,G
R5102	T8063	T8060	appos	G,Homer3
R5103	T8064	T8063	compound	CTC,G
R5104	T8065	T8063	compound	TAC,G
R5105	T8066	T8063	compound	ACA,G
R5106	T8067	T8063	compound	GTG,G
R5107	T8068	T8063	compound	CAA,G
R5108	T8069	T8063	compound	AGC,G
R5109	T8070	T8063	compound	TCA,G
R511	T1105	T1104	dobj	hyposmia,included
R5110	T8071	T8028	punct	;,Pax6
R5111	T8072	T8028	appos	Trim11,Pax6
R5112	T8073	T8072	punct	-,Trim11
R5113	T8074	T8075	compound	F,CTG
R5114	T8075	T8072	appos	CTG,Trim11
R5115	T8076	T8075	compound	GTG,CTG
R5116	T8077	T8075	compound	CAG,CTG
R5117	T8078	T8075	compound	GAT,CTG
R5118	T8079	T8075	compound	GTG,CTG
R5119	T8080	T8075	compound	AAG,CTG
R512	T1106	T1105	cc	and,hyposmia
R5120	T8081	T8028	punct	;,Pax6
R5121	T8082	T8028	appos	Trim11,Pax6
R5122	T8083	T8082	punct	-,Trim11
R5123	T8084	T8085	compound	R,GG
R5124	T8085	T8082	appos	GG,Trim11
R5125	T8086	T8085	compound	GCC,GG
R5126	T8087	T8085	compound	TGC,GG
R5127	T8088	T8085	compound	AGA,GG
R5128	T8089	T8085	compound	TAG,GG
R5129	T8090	T8085	compound	TCA,GG
R513	T1107	T1108	amod	abnormal,transfer
R5130	T8091	T8085	compound	TAG,GG
R5131	T8092	T8028	punct	;,Pax6
R5132	T8093	T8028	appos	Dncl1,Pax6
R5133	T8094	T8093	punct	-,Dncl1
R5134	T8095	T8096	compound	F,G
R5135	T8096	T8093	appos	G,Dncl1
R5136	T8097	T8096	compound	CAA,G
R5137	T8098	T8096	compound	AAA,G
R5138	T8099	T8096	compound	TGC,G
R5139	T8100	T8096	compound	AGA,G
R514	T1108	T1105	conj	transfer,hyposmia
R5140	T8101	T8096	compound	CAT,G
R5141	T8102	T8096	compound	GTC,G
R5142	T8103	T8028	punct	;,Pax6
R5143	T8104	T8028	appos	Dncl1,Pax6
R5144	T8105	T8104	punct	-,Dncl1
R5145	T8106	T8107	compound	R,GGG
R5146	T8107	T8104	appos	GGG,Dncl1
R5147	T8108	T8107	compound	CTA,GGG
R5148	T8109	T8107	compound	AGG,GGG
R5149	T8110	T8107	compound	GAG,GGG
R515	T1109	T1108	amod	inter-hemispheric,transfer
R5150	T8111	T8107	compound	AAA,GGG
R5151	T8112	T8107	compound	AAA,GGG
R5152	T8113	T8107	compound	ATG,GGG
R5153	T8114	T8028	punct	;,Pax6
R5154	T8115	T8116	compound	Gapdh,F
R5155	T8116	T8028	appos	F,Pax6
R5156	T8117	T8116	punct	-,F
R5157	T8118	T8116	punct	: ,F
R5158	T8119	T8120	compound	CAT,GC
R5159	T8120	T8116	appos	GC,F
R516	T1110	T1108	amod	auditory,transfer
R5160	T8121	T8120	compound	CAC,GC
R5161	T8122	T8120	compound	CAT,GC
R5162	T8123	T8120	compound	CTT,GC
R5163	T8124	T8120	compound	CCA,GC
R5164	T8125	T8120	compound	GGA,GC
R5165	T8126	T8028	punct	;,Pax6
R5166	T8127	T8128	compound	Gapdh,R
R5167	T8128	T8028	appos	R,Pax6
R5168	T8129	T8128	punct	-,R
R5169	T8130	T8128	punct	: ,R
R517	T1111	T1112	punct	[,14
R5170	T8131	T8132	compound	ATG,TT
R5171	T8132	T8128	appos	TT,R
R5172	T8133	T8132	compound	ACC,TT
R5173	T8134	T8132	compound	TTG,TT
R5174	T8135	T8132	compound	CCC,TT
R5175	T8136	T8132	compound	ACA,TT
R5176	T8137	T8132	compound	GCC,TT
R5177	T8138	T8028	punct	;,Pax6
R5178	T8139	T8140	compound	Atp5a1,F
R5179	T8140	T8028	appos	F,Pax6
R518	T1112	T1104	parataxis	14,included
R5180	T8141	T8140	punct	-,F
R5181	T8142	T8140	punct	: ,F
R5182	T8143	T8144	compound	CAC,CA
R5183	T8144	T8140	appos	CA,F
R5184	T8145	T8144	compound	ACG,CA
R5185	T8146	T8144	compound	TGA,CA
R5186	T8147	T8144	compound	GAT,CA
R5187	T8148	T8144	compound	GTC,CA
R5188	T8149	T8144	compound	CTC,CA
R5189	T8150	T8028	punct	;,Pax6
R519	T1113	T1112	nummod	11,14
R5190	T8151	T8152	compound	Atp5a1,R
R5191	T8152	T8028	appos	R,Pax6
R5192	T8153	T8152	punct	-,R
R5193	T8154	T8152	punct	: ,R
R5194	T8155	T8156	compound	CAC,A
R5195	T8156	T8152	appos	A,R
R5196	T8157	T8156	compound	AGA,A
R5197	T8158	T8156	compound	GAT,A
R5198	T8159	T8156	compound	TCG,A
R5199	T8160	T8156	compound	GGG,A
R52	T235	T236	det	the,eye
R520	T1114	T1112	punct	",",14
R5200	T8161	T8156	compound	ATA,A
R5201	T8162	T8026	punct	.,were
R5202	T8164	T8165	nummod	10,ng
R5203	T8165	T8166	compound	ng,cDNA
R5204	T8166	T8168	nsubjpass	cDNA,amplified
R5205	T8167	T8166	compound	library,cDNA
R5206	T8169	T8168	auxpass	were,amplified
R5207	T8170	T8168	prep	in,amplified
R5208	T8171	T8172	det	a,reaction
R5209	T8172	T8170	pobj	reaction,in
R521	T1115	T1112	punct	],14
R5210	T8173	T8172	acl	containing,reaction
R5211	T8174	T8175	nummod	1,buffer
R5212	T8175	T8173	dobj	buffer,containing
R5213	T8176	T8174	punct	x,1
R5214	T8177	T8175	compound	AmpliTaq,buffer
R5215	T8178	T8175	compound	polymerase,buffer
R5216	T8179	T8180	punct	(,Elmer
R5217	T8180	T8175	parataxis	Elmer,buffer
R5218	T8181	T8180	compound	Perkin,Elmer
R5219	T8182	T8180	punct	),Elmer
R522	T1116	T1104	punct	.,included
R5220	T8183	T8175	punct	", ",buffer
R5221	T8184	T8185	nummod	1.5,mM
R5222	T8185	T8186	compound	mM,MgCl2
R5223	T8186	T8175	conj	MgCl2,buffer
R5224	T8187	T8186	punct	", ",MgCl2
R5225	T8188	T8189	nmod	200μM,primer
R5226	T8189	T8186	conj	primer,MgCl2
R5227	T8190	T8189	det	each,primer
R5228	T8191	T8189	cc	and,primer
R5229	T8192	T8193	nummod	2.5,units
R523	T1118	T1119	det	The,feature
R5230	T8193	T8189	conj	units,primer
R5231	T8194	T8193	prep	of,units
R5232	T8195	T8196	compound	AmpliTaq,polymerase
R5233	T8196	T8194	pobj	polymerase,of
R5234	T8197	T8198	punct	(,Elmer
R5235	T8198	T8196	parataxis	Elmer,polymerase
R5236	T8199	T8198	compound	Perkin,Elmer
R5237	T8200	T8198	punct	),Elmer
R5238	T8201	T8168	punct	.,amplified
R5239	T8203	T8204	compound	PCR,conditions
R524	T1119	T1121	nsubj	feature,is
R5240	T8204	T8205	nsubj	conditions,were
R5241	T8206	T8205	punct	(,were
R5242	T8207	T8208	nummod	95,°C
R5243	T8208	T8205	attr	°C,were
R5244	T8209	T8208	prep	for,°C
R5245	T8210	T8211	nummod	30,sec
R5246	T8211	T8209	pobj	sec,for
R5247	T8212	T8208	punct	),°C
R5248	T8213	T8208	punct	×,°C
R5249	T8214	T8208	appos	1,°C
R525	T1120	T1119	amod	defining,feature
R5250	T8215	T8208	punct	", ",°C
R5251	T8216	T8217	punct	(,°C
R5252	T8217	T8208	conj	°C,°C
R5253	T8218	T8217	nummod	94,°C
R5254	T8219	T8217	prep	for,°C
R5255	T8220	T8221	nummod	30,sec
R5256	T8221	T8219	pobj	sec,for
R5257	T8222	T8217	punct	", ",°C
R5258	T8223	T8224	nummod	55,°C
R5259	T8224	T8217	appos	°C,°C
R526	T1122	T1119	prep	of,feature
R5260	T8225	T8224	prep	for,°C
R5261	T8226	T8227	nummod	30,sec
R5262	T8227	T8225	pobj	sec,for
R5263	T8228	T8217	punct	", ",°C
R5264	T8229	T8230	nummod	72,°C
R5265	T8230	T8217	appos	°C,°C
R5266	T8231	T8230	prep	for,°C
R5267	T8232	T8233	nummod	30,sec
R5268	T8233	T8231	pobj	sec,for
R5269	T8234	T8217	punct	),°C
R527	T1123	T1124	det	all,proteins
R5270	T8235	T8217	punct	×,°C
R5271	T8236	T8217	appos	32,°C
R5272	T8237	T8217	cc	and,°C
R5273	T8238	T8239	punct	(,°C
R5274	T8239	T8217	conj	°C,°C
R5275	T8240	T8239	nummod	72,°C
R5276	T8241	T8239	prep	for,°C
R5277	T8242	T8243	nummod	2,min
R5278	T8243	T8241	pobj	min,for
R5279	T8244	T8239	punct	),°C
R528	T1124	T1122	pobj	proteins,of
R5280	T8245	T8239	punct	×,°C
R5281	T8246	T8239	appos	1,°C
R5282	T8247	T8205	punct	.,were
R5283	T8249	T8250	nsubjpass	Products,resolved
R5284	T8251	T8250	auxpass	were,resolved
R5285	T8252	T8250	prep	on,resolved
R5286	T8253	T8254	det	a,gel
R5287	T8254	T8252	pobj	gel,on
R5288	T8255	T8256	nummod	2.5,%
R5289	T8256	T8254	compound	%,gel
R529	T1125	T1124	compound	PAX,proteins
R5290	T8257	T8254	compound	agarose,gel
R5291	T8258	T8250	prep	with,resolved
R5292	T8259	T8260	compound	ΦX174,HaeIII
R5293	T8260	T8262	compound	HaeIII,markers
R5294	T8261	T8260	punct	/,HaeIII
R5295	T8262	T8258	pobj	markers,with
R5296	T8263	T8262	compound	size,markers
R5297	T8264	T8265	punct	(,Promega
R5298	T8265	T8262	parataxis	Promega,markers
R5299	T8266	T8265	punct	),Promega
R53	T236	T234	pobj	eye,of
R530	T1126	T1127	det	the,presence
R5300	T8267	T8250	punct	.,resolved
R5301	T8300	T8299	prep	of,Characterisation
R5302	T8301	T8302	det	the,peptide
R5303	T8302	T8300	pobj	peptide,of
R5304	T8303	T8302	nmod	PAX6,peptide
R5305	T8304	T8305	npadvmod	C,terminal
R5306	T8305	T8302	amod	terminal,peptide
R5307	T8306	T8305	punct	-,terminal
R5308	T8307	T8299	punct	.,Characterisation
R5309	T8309	T8310	punct	(,a
R531	T1127	T1121	attr	presence,is
R5310	T8310	T8311	meta	a,alignment
R5311	T8312	T8310	punct	),a
R5312	T8313	T8311	compound	CLUSTAL,alignment
R5313	T8314	T8311	prep	of,alignment
R5314	T8315	T8316	det	the,acids
R5315	T8316	T8314	pobj	acids,of
R5316	T8317	T8316	amod	terminal,acids
R5317	T8318	T8316	nummod	42,acids
R5318	T8319	T8316	compound	amino,acids
R5319	T8320	T8316	prep	of,acids
R532	T1128	T1127	prep	of,presence
R5320	T8321	T8320	pobj	PAX6,of
R5321	T8322	T8316	prep	from,acids
R5322	T8323	T8324	amod	diverse,species
R5323	T8324	T8322	pobj	species,from
R5324	T8325	T8311	punct	.,alignment
R5325	T8327	T8328	punct	(,indicates
R5326	T8328	T8331	ccomp	indicates,indicates
R5327	T8329	T8328	nsubj	*,indicates
R5328	T8330	T8328	punct	),indicates
R5329	T8332	T8333	amod	invariant,residues
R533	T1129	T1130	det	a,domain
R5330	T8333	T8328	dobj	residues,indicates
R5331	T8334	T8328	punct	", ",indicates
R5332	T8335	T8328	punct	(,indicates
R5333	T8336	T8331	nsubj	:,indicates
R5334	T8337	T8331	punct	),indicates
R5335	T8338	T8339	advmod	highly,similar
R5336	T8339	T8340	amod	similar,substitutions
R5337	T8340	T8331	dobj	substitutions,indicates
R5338	T8341	T8331	cc	and,indicates
R5339	T8342	T8343	punct	(,indicates
R534	T1130	T1128	pobj	domain,of
R5340	T8343	T8331	conj	indicates,indicates
R5341	T8344	T8343	nsubj	.,indicates
R5342	T8345	T8343	punct	),indicates
R5343	T8346	T8347	advmod	moderately,similar
R5344	T8347	T8348	amod	similar,substitutions
R5345	T8348	T8343	dobj	substitutions,indicates
R5346	T8349	T8343	punct	.,indicates
R5347	T8351	T8352	punct	(,b
R5348	T8352	T8353	meta	b,predicts
R5349	T8354	T8352	punct	),b
R535	T1131	T1132	nummod	128,acid
R5350	T8355	T8356	amod	Secondary,analysis
R5351	T8356	T8353	nsubj	analysis,predicts
R5352	T8357	T8356	compound	structure,analysis
R5353	T8358	T8356	prep	of,analysis
R5354	T8359	T8360	det	the,acids
R5355	T8360	T8358	pobj	acids,of
R5356	T8361	T8362	advmod	highly,conserved
R5357	T8362	T8360	amod	conserved,acids
R5358	T8363	T8360	amod	terminal,acids
R5359	T8364	T8360	nummod	28,acids
R536	T1132	T1130	nmod	acid,domain
R5360	T8365	T8360	compound	amino,acids
R5361	T8366	T8367	det	a,sheet
R5362	T8367	T8353	dobj	sheet,predicts
R5363	T8368	T8367	amod	single,sheet
R5364	T8369	T8367	compound	beta,sheet
R5365	T8370	T8371	punct	(,arrow
R5366	T8371	T8367	parataxis	arrow,sheet
R5367	T8372	T8371	punct	),arrow
R5368	T8373	T8353	prep	in,predicts
R5369	T8374	T8375	det	the,peptide
R537	T1133	T1132	nmod	amino,acid
R5370	T8375	T8373	pobj	peptide,in
R5371	T8376	T8375	compound	SVPVQ,peptide
R5372	T8377	T8353	punct	.,predicts
R5373	T8379	T8380	punct	(,c
R5374	T8380	T8381	meta	c,predicted
R5375	T8382	T8380	punct	),c
R5376	T8383	T8384	advmod	When,added
R5377	T8384	T8381	advcl	added,predicted
R5378	T8385	T8386	nummod	4,residues
R5379	T8386	T8384	nsubjpass	residues,added
R538	T1134	T1135	npadvmod	DNA,binding
R5380	T8387	T8384	auxpass	are,added
R5381	T8388	T8384	prep	in,added
R5382	T8389	T8390	det	the,direction
R5383	T8390	T8388	pobj	direction,in
R5384	T8391	T8392	npadvmod	N,terminal
R5385	T8392	T8390	amod	terminal,direction
R5386	T8393	T8392	punct	-,terminal
R5387	T8394	T8381	punct	", ",predicted
R5388	T8395	T8396	nummod	2,sheets
R5389	T8396	T8381	nsubjpass	sheets,predicted
R539	T1135	T1130	amod	binding,domain
R5390	T8397	T8396	compound	beta,sheets
R5391	T8398	T8381	auxpass	are,predicted
R5392	T8399	T8381	advmod	now,predicted
R5393	T8400	T8381	punct	.,predicted
R5394	T8468	T8469	amod	Semi-quantitative,analysis
R5395	T8470	T8469	compound	PCR,analysis
R5396	T8471	T8469	prep	of,analysis
R5397	T8472	T8471	pobj	Homer3,of
R5398	T8473	T8472	punct	", ",Homer3
R5399	T8474	T8472	conj	Dncl1,Homer3
R54	T237	T236	cc	and,eye
R540	T1136	T1135	punct	-,binding
R5400	T8475	T8474	punct	", ",Dncl1
R5401	T8476	T8474	conj	Trim11,Dncl1
R5402	T8477	T8476	cc	and,Trim11
R5403	T8478	T8476	conj	Pax6,Trim11
R5404	T8479	T8469	prep	in,analysis
R5405	T8480	T8481	det	the,library
R5406	T8481	T8479	pobj	library,in
R5407	T8482	T8483	compound	mouse,brain
R5408	T8483	T8481	compound	brain,library
R5409	T8484	T8481	compound	cDNA,library
R541	T1137	T1130	amod	paired,domain
R5410	T8485	T8469	punct	.,analysis
R5411	T8487	T8488	compound	Library,cDNA
R5412	T8488	T8489	nsubjpass	cDNA,amplified
R5413	T8490	T8489	auxpass	was,amplified
R5414	T8491	T8489	prep	with,amplified
R5415	T8492	T8491	pobj	primers,with
R5416	T8493	T8492	amod	specific,primers
R5417	T8494	T8493	prep	for,specific
R5418	T8495	T8494	pobj	Pax6,for
R5419	T8496	T8497	punct	(,bp
R542	T1138	T1139	dep	that,conserved
R5420	T8497	T8495	parataxis	bp,Pax6
R5421	T8498	T8497	nummod	600,bp
R5422	T8499	T8497	punct	),bp
R5423	T8500	T8495	punct	", ",Pax6
R5424	T8501	T8495	conj	Homer3,Pax6
R5425	T8502	T8503	punct	(,band
R5426	T8503	T8501	parataxis	band,Homer3
R5427	T8504	T8505	nummod	485,bp
R5428	T8505	T8503	compound	bp,band
R5429	T8506	T8503	punct	),band
R543	T1139	T1130	relcl	conserved,domain
R5430	T8507	T8501	punct	", ",Homer3
R5431	T8508	T8501	conj	Dncl1,Homer3
R5432	T8509	T8510	punct	(,bp
R5433	T8510	T8508	parataxis	bp,Dncl1
R5434	T8511	T8510	nummod	485,bp
R5435	T8512	T8510	punct	),bp
R5436	T8513	T8508	cc	and,Dncl1
R5437	T8514	T8508	conj	Trim11,Dncl1
R5438	T8515	T8516	punct	(,bp
R5439	T8516	T8514	parataxis	bp,Trim11
R544	T1140	T1139	aux	has,conserved
R5440	T8517	T8516	nummod	609,bp
R5441	T8518	T8516	punct	),bp
R5442	T8519	T8489	prep	for,amplified
R5443	T8520	T8521	nummod	20,cycles
R5444	T8521	T8519	pobj	cycles,for
R5445	T8522	T8520	punct	", ",20
R5446	T8523	T8520	conj	25,20
R5447	T8524	T8523	punct	", ",25
R5448	T8525	T8523	conj	30,25
R5449	T8526	T8525	cc	or,30
R545	T1141	T1139	auxpass	been,conserved
R5450	T8527	T8525	conj	35,30
R5451	T8528	T8489	punct	.,amplified
R5452	T8530	T8531	nsubjpass	Atp5a1,included
R5453	T8532	T8533	punct	(,bp
R5454	T8533	T8530	parataxis	bp,Atp5a1
R5455	T8534	T8533	nummod	415,bp
R5456	T8535	T8533	punct	),bp
R5457	T8536	T8530	cc	and,Atp5a1
R5458	T8537	T8530	conj	Gapdh,Atp5a1
R5459	T8538	T8539	punct	(,bp
R546	T1142	T1139	advmod	highly,conserved
R5460	T8539	T8537	parataxis	bp,Gapdh
R5461	T8540	T8539	nummod	450,bp
R5462	T8541	T8539	punct	),bp
R5463	T8542	T8530	punct	", ",Atp5a1
R5464	T8543	T8544	dep	which,expressed
R5465	T8544	T8530	relcl	expressed,Atp5a1
R5466	T8545	T8544	auxpass	are,expressed
R5467	T8546	T8544	advmod	highly,expressed
R5468	T8547	T8544	prep	in,expressed
R5469	T8548	T8549	det	the,brain
R547	T1143	T1139	prep	over,conserved
R5470	T8549	T8547	pobj	brain,in
R5471	T8550	T8531	punct	", ",included
R5472	T8551	T8531	auxpass	are,included
R5473	T8552	T8531	prep	for,included
R5474	T8553	T8552	pobj	comparison,for
R5475	T8554	T8531	punct	.,included
R5476	T8556	T8557	nsubj	M,indicates
R5477	T8557	T8558	ccomp	indicates,indicated
R5478	T8559	T8560	det	the,marker
R5479	T8560	T8557	dobj	marker,indicates
R548	T1144	T1143	pobj	evolution,over
R5480	T8561	T8562	nmod	Φ×,HaeIII
R5481	T8562	T8560	compound	HaeIII,marker
R5482	T8563	T8562	nummod	174,HaeIII
R5483	T8564	T8565	compound	DNA,size
R5484	T8565	T8560	compound	size,marker
R5485	T8566	T8558	punct	;,indicated
R5486	T8567	T8568	det	the,positions
R5487	T8568	T8558	nsubjpass	positions,indicated
R5488	T8569	T8568	prep	of,positions
R5489	T8570	T8571	det	the,bands
R549	T1145	T1146	punct	[,1
R5490	T8571	T8569	pobj	bands,of
R5491	T8572	T8573	nummod	603,bp
R5492	T8573	T8571	nmod	bp,bands
R5493	T8574	T8573	cc	and,bp
R5494	T8575	T8576	nummod	310,bp
R5495	T8576	T8573	conj	bp,bp
R5496	T8577	T8571	compound	marker,bands
R5497	T8578	T8558	auxpass	are,indicated
R5498	T8579	T8558	punct	.,indicated
R5499	T8632	T8633	compound	LacZ,activation
R55	T238	T236	conj	brain,eye
R550	T1146	T1127	parataxis	1,presence
R5500	T8634	T8635	compound	reporter,gene
R5501	T8635	T8633	compound	gene,activation
R5502	T8636	T8633	prep	in,activation
R5503	T8637	T8638	amod	pairwise,tests
R5504	T8638	T8636	pobj	tests,in
R5505	T8639	T8633	punct	.,activation
R5506	T8641	T8642	compound	pPC86,constructs
R5507	T8642	T8643	nsubjpass	constructs,shown
R5508	T8644	T8643	auxpass	are,shown
R5509	T8645	T8643	prep	across,shown
R551	T1147	T1146	punct	],1
R5510	T8646	T8647	det	the,top
R5511	T8647	T8645	pobj	top,across
R5512	T8648	T8643	punct	", ",shown
R5513	T8649	T8643	cc	and,shown
R5514	T8650	T8651	compound	pDBLeu,constructs
R5515	T8651	T8652	nsubjpass	constructs,shown
R5516	T8652	T8643	conj	shown,shown
R5517	T8653	T8652	auxpass	are,shown
R5518	T8654	T8652	prep	down,shown
R5519	T8655	T8656	det	the,side
R552	T1148	T1121	punct	.,is
R5520	T8656	T8654	pobj	side,down
R5521	T8657	T8658	amod	right,hand
R5522	T8658	T8656	compound	hand,side
R5523	T8659	T8652	punct	.,shown
R5524	T8662	T8661	punct	", ",PAX6PST
R5525	T8663	T8664	compound	PAX6,domain
R5526	T8664	T8661	appos	domain,PAX6PST
R5527	T8665	T8664	compound	PST,domain
R5528	T8666	T8661	punct	;,PAX6PST
R5529	T8667	T8668	compound	PAX6PST,Q422R
R553	T1150	T1151	prep	In,contains
R5530	T8668	T8661	appos	Q422R,PAX6PST
R5531	T8669	T8668	punct	/,Q422R
R5532	T8670	T8668	punct	", ",Q422R
R5533	T8671	T8672	compound	PAX6,domain
R5534	T8672	T8668	appos	domain,Q422R
R5535	T8673	T8672	compound	PST,domain
R5536	T8674	T8672	prep	with,domain
R5537	T8675	T8676	det	the,mutation
R5538	T8676	T8674	pobj	mutation,with
R5539	T8677	T8676	compound	Q422R,mutation
R554	T1152	T1150	pobj	addition,In
R5540	T8678	T8661	punct	;,PAX6PST
R5541	T8679	T8680	compound	PAX6PST,X423L
R5542	T8680	T8661	appos	X423L,PAX6PST
R5543	T8681	T8680	punct	/,X423L
R5544	T8682	T8680	punct	", ",X423L
R5545	T8683	T8684	compound	PAX6,domain
R5546	T8684	T8680	appos	domain,X423L
R5547	T8685	T8684	compound	PST,domain
R5548	T8686	T8684	prep	with,domain
R5549	T8687	T8688	det	the,mutation
R555	T1153	T1152	prep	to,addition
R5550	T8688	T8686	pobj	mutation,with
R5551	T8689	T8688	compound	X423L,mutation
R5552	T8690	T8661	punct	", ",PAX6PST
R5553	T8691	T8692	compound	PAX6PST,1615del10
R5554	T8692	T8661	appos	1615del10,PAX6PST
R5555	T8693	T8692	punct	/,1615del10
R5556	T8694	T8692	punct	", ",1615del10
R5557	T8695	T8696	compound	PAX6,domain
R5558	T8696	T8692	appos	domain,1615del10
R5559	T8697	T8696	compound	PST,domain
R556	T1154	T1155	det	the,domain
R5560	T8698	T8696	prep	with,domain
R5561	T8699	T8700	det	the,mutation
R5562	T8700	T8698	pobj	mutation,with
R5563	T8701	T8700	nummod	1615,mutation
R5564	T8702	T8700	compound	del10,mutation
R5565	T8703	T8661	punct	;,PAX6PST
R5566	T8704	T8705	compound	PAX6PST,CT
R5567	T8705	T8661	appos	CT,PAX6PST
R5568	T8706	T8705	punct	-,CT
R5569	T8707	T8705	punct	", ",CT
R557	T1155	T1153	pobj	domain,to
R5570	T8708	T8709	compound	PAX6,PST
R5571	T8709	T8710	compound	PST,domain
R5572	T8710	T8705	appos	domain,CT
R5573	T8711	T8710	cc	minus,domain
R5574	T8712	T8713	det	the,peptide
R5575	T8713	T8710	conj	peptide,domain
R5576	T8714	T8715	npadvmod	C,terminal
R5577	T8715	T8713	amod	terminal,peptide
R5578	T8716	T8715	punct	-,terminal
R5579	T8717	T8661	punct	.,PAX6PST
R558	T1156	T1155	amod	paired,domain
R5580	T8719	T8720	punct	',is
R5581	T8721	T8722	amod	Truncated,HOMER3
R5582	T8722	T8720	nsubj	HOMER3,is
R5583	T8723	T8720	punct	',is
R5584	T8724	T8720	attr	HOMER3,is
R5585	T8725	T8724	acl	lacking,HOMER3
R5586	T8726	T8727	det	the,acids
R5587	T8727	T8725	dobj	acids,lacking
R5588	T8728	T8729	npadvmod	N,terminal
R5589	T8729	T8727	amod	terminal,acids
R559	T1157	T1151	punct	", ",contains
R5590	T8730	T8729	punct	-,terminal
R5591	T8731	T8727	nummod	70,acids
R5592	T8732	T8727	compound	amino,acids
R5593	T8733	T8720	punct	.,is
R5594	T8735	T8736	nummod	Five,strains
R5595	T8736	T8738	nsubjpass	strains,shown
R5596	T8737	T8736	compound	control,strains
R5597	T8739	T8738	auxpass	are,shown
R5598	T8740	T8738	prep	for,shown
R5599	T8741	T8740	pobj	comparison,for
R56	T239	T220	punct	.,have
R560	T1158	T1151	nsubj	PAX6,contains
R5600	T8742	T8743	punct	(,left
R5601	T8743	T8738	parataxis	left,shown
R5602	T8744	T8743	punct	),left
R5603	T8745	T8738	punct	.,shown
R5604	T8747	T8748	nsubj	These,range
R5605	T8749	T8748	prep	from,range
R5606	T8750	T8749	pobj	non-interactor,from
R5607	T8751	T8752	punct	(,A
R5608	T8752	T8750	parataxis	A,non-interactor
R5609	T8753	T8752	punct	),A
R561	T1159	T1151	advmod	also,contains
R5610	T8754	T8749	prep	to,from
R5611	T8755	T8756	amod	strong,interactor
R5612	T8756	T8754	pobj	interactor,to
R5613	T8757	T8758	punct	(,E
R5614	T8758	T8756	parataxis	E,interactor
R5615	T8759	T8758	punct	),E
R5616	T8760	T8748	punct	.,range
R5617	T8837	T8838	amod	Pairwise,tests
R5618	T8839	T8838	compound	interaction,tests
R5619	T8840	T8838	prep	between,tests
R562	T1160	T1161	det	a,homeodomain
R5620	T8841	T8842	amod	normal,constructs
R5621	T8842	T8840	pobj	constructs,between
R5622	T8843	T8841	cc	and,normal
R5623	T8844	T8841	conj	mutant,normal
R5624	T8845	T8842	compound	PAX6,constructs
R5625	T8846	T8842	cc	and,constructs
R5626	T8847	T8842	conj	HOMER3,constructs
R5627	T8848	T8847	punct	", ",HOMER3
R5628	T8849	T8847	conj	DNCL1,HOMER3
R5629	T8850	T8849	cc	and,DNCL1
R563	T1161	T1151	dobj	homeodomain,contains
R5630	T8851	T8849	conj	TRIM11,DNCL1
R5631	T8852	T8838	punct	.,tests
R5632	T8855	T8854	punct	: ,+++
R5633	T8856	T8857	amod	strong,interaction
R5634	T8857	T8854	appos	interaction,+++
R5635	T8858	T8854	punct	;,+++
R5636	T8859	T8854	appos	++,+++
R5637	T8860	T8859	punct	: ,++
R5638	T8861	T8862	amod	moderate,interaction
R5639	T8862	T8859	appos	interaction,++
R564	T1162	T1163	npadvmod	DNA,binding
R5640	T8863	T8854	punct	;,+++
R5641	T8864	T8854	appos	+,+++
R5642	T8865	T8864	punct	: ,+
R5643	T8866	T8867	amod	weak,interaction
R5644	T8867	T8864	appos	interaction,+
R5645	T8868	T8854	punct	;,+++
R5646	T8869	T8870	punct	(,)
R5647	T8870	T8854	appos	),+++
R5648	T8871	T8870	punct	+,)
R5649	T8872	T8873	amod	borderline,interaction
R565	T1163	T1161	amod	binding,homeodomain
R5650	T8873	T8870	appos	interaction,)
R5651	T8874	T8873	prep	with,interaction
R5652	T8875	T8876	nummod	one,reporters
R5653	T8876	T8874	pobj	reporters,with
R5654	T8877	T8875	cc	or,one
R5655	T8878	T8875	conj	two,one
R5656	T8879	T8876	acl	activated,reporters
R5657	T8880	T8879	prep	at,activated
R5658	T8881	T8882	advmod	very,low
R5659	T8882	T8883	amod	low,levels
R566	T1164	T1163	punct	-,binding
R5660	T8883	T8880	pobj	levels,at
R5661	T8884	T8854	punct	;,+++
R5662	T8885	T8854	appos	0,+++
R5663	T8886	T8885	punct	: ,0
R5664	T8887	T8888	det	no,interaction
R5665	T8888	T8885	appos	interaction,0
R5666	T8889	T8854	punct	.,+++
R5667	T8892	T8891	punct	", ",PAX6PST
R5668	T8893	T8894	compound	PAX6,domain
R5669	T8894	T8891	appos	domain,PAX6PST
R567	T1165	T1161	cc	and,homeodomain
R5670	T8895	T8894	compound	PST,domain
R5671	T8896	T8891	punct	;,PAX6PST
R5672	T8897	T8891	appos	PAX6CTP,PAX6PST
R5673	T8898	T8897	punct	", ",PAX6CTP
R5674	T8899	T8900	nmod	PAX6,peptide
R5675	T8900	T8897	appos	peptide,PAX6CTP
R5676	T8901	T8902	npadvmod	C,terminal
R5677	T8902	T8900	amod	terminal,peptide
R5678	T8903	T8902	punct	-,terminal
R5679	T8904	T8891	punct	;,PAX6PST
R568	T1166	T1167	det	a,domain
R5680	T8905	T8906	compound	PAX6PST,CT
R5681	T8906	T8891	appos	CT,PAX6PST
R5682	T8907	T8906	punct	-,CT
R5683	T8908	T8906	punct	", ",CT
R5684	T8909	T8910	compound	PAX6,domain
R5685	T8910	T8906	appos	domain,CT
R5686	T8911	T8910	compound	PST,domain
R5687	T8912	T8910	cc	minus,domain
R5688	T8913	T8914	det	the,peptide
R5689	T8914	T8910	conj	peptide,domain
R569	T1167	T1161	conj	domain,homeodomain
R5690	T8915	T8916	npadvmod	C,terminal
R5691	T8916	T8914	amod	terminal,peptide
R5692	T8917	T8916	punct	-,terminal
R5693	T8918	T8891	punct	;,PAX6PST
R5694	T8919	T8920	compound	PAX6PST,Q422R
R5695	T8920	T8891	appos	Q422R,PAX6PST
R5696	T8921	T8920	punct	/,Q422R
R5697	T8922	T8920	punct	", ",Q422R
R5698	T8923	T8924	compound	PAX6,domain
R5699	T8924	T8920	appos	domain,Q422R
R57	T241	T242	nsubjpass	Little,known
R570	T1168	T1169	npadvmod	proline,rich
R5700	T8925	T8924	compound	PST,domain
R5701	T8926	T8924	prep	with,domain
R5702	T8927	T8928	det	the,mutation
R5703	T8928	T8926	pobj	mutation,with
R5704	T8929	T8928	compound	Q422R,mutation
R5705	T8930	T8891	punct	;,PAX6PST
R5706	T8931	T8932	compound	PAX6PST,X423L
R5707	T8932	T8891	appos	X423L,PAX6PST
R5708	T8933	T8932	punct	/,X423L
R5709	T8934	T8932	punct	", ",X423L
R571	T1169	T1167	amod	rich,domain
R5710	T8935	T8936	compound	PAX6,domain
R5711	T8936	T8932	appos	domain,X423L
R5712	T8937	T8936	compound	PST,domain
R5713	T8938	T8936	prep	with,domain
R5714	T8939	T8940	det	the,mutation
R5715	T8940	T8938	pobj	mutation,with
R5716	T8941	T8940	compound	X423L,mutation
R5717	T8942	T8891	punct	", ",PAX6PST
R5718	T8943	T8944	compound	PAX6PST,1615del
R5719	T8944	T8891	appos	1615del,PAX6PST
R572	T1170	T1168	punct	", ",proline
R5720	T8945	T8944	punct	/,1615del
R5721	T8946	T8944	punct	", ",1615del
R5722	T8947	T8948	compound	PAX6,domain
R5723	T8948	T8944	appos	domain,1615del
R5724	T8949	T8948	compound	PST,domain
R5725	T8950	T8948	prep	with,domain
R5726	T8951	T8952	det	the,mutation
R5727	T8952	T8950	pobj	mutation,with
R5728	T8953	T8952	nummod	1615,mutation
R5729	T8954	T8952	compound	del10,mutation
R573	T1171	T1168	conj	serine,proline
R5730	T8955	T8891	punct	.,PAX6PST
R5731	T8957	T8958	compound	HOMER3,FL
R5732	T8959	T8958	punct	-,FL
R5733	T8960	T8958	punct	", ",FL
R5734	T8961	T8962	nmod	HOMER3,clone
R5735	T8962	T8958	appos	clone,FL
R5736	T8963	T8964	amod	full,length
R5737	T8964	T8962	compound	length,clone
R5738	T8965	T8964	punct	-,length
R5739	T8966	T8958	punct	;,FL
R574	T1172	T1171	cc	and,serine
R5740	T8967	T8968	compound	HOMER3,Tr
R5741	T8968	T8958	appos	Tr,FL
R5742	T8969	T8968	punct	-,Tr
R5743	T8970	T8968	punct	", ",Tr
R5744	T8971	T8972	amod	truncated,clone
R5745	T8972	T8968	appos	clone,Tr
R5746	T8973	T8972	compound	HOMER3,clone
R5747	T8974	T8972	acl	lacking,clone
R5748	T8975	T8976	det	the,acids
R5749	T8976	T8974	dobj	acids,lacking
R575	T1173	T1171	conj	threonine,serine
R5750	T8977	T8978	npadvmod	N,terminal
R5751	T8978	T8976	amod	terminal,acids
R5752	T8979	T8978	punct	-,terminal
R5753	T8980	T8976	nummod	70,acids
R5754	T8981	T8976	compound	amino,acids
R5755	T8982	T8958	punct	.,FL
R576	T1174	T1169	punct	-,rich
R577	T1175	T1176	punct	(,PST
R578	T1176	T1167	parataxis	PST,domain
R579	T1177	T1176	punct	),PST
R58	T243	T242	auxpass	is,known
R580	T1178	T1151	prep	at,contains
R581	T1179	T1180	det	the,terminus
R582	T1180	T1178	pobj	terminus,at
R583	T1181	T1180	compound	C,terminus
R584	T1182	T1180	punct	-,terminus
R585	T1183	T1184	punct	[,6
R586	T1184	T1151	parataxis	6,contains
R587	T1185	T1184	nummod	1,6
R588	T1186	T1184	punct	",",6
R589	T1187	T1184	punct	],6
R59	T244	T242	prep	about,known
R590	T1188	T1151	punct	.,contains
R591	T1190	T1191	det	The,domain
R592	T1191	T1193	nsubjpass	domain,shown
R593	T1192	T1191	compound	PST,domain
R594	T1194	T1191	punct	", ",domain
R595	T1195	T1196	dep	which,encompasses
R596	T1196	T1191	relcl	encompasses,domain
R597	T1197	T1198	det	the,acids
R598	T1198	T1196	dobj	acids,encompasses
R599	T1199	T1200	npadvmod	C,terminal
R6	T182	T180	relcl	interact,proteins
R60	T245	T246	det	the,interactions
R600	T1200	T1198	amod	terminal,acids
R601	T1201	T1200	punct	-,terminal
R602	T1202	T1198	nummod	145,acids
R603	T1203	T1198	compound	amino,acids
R604	T1204	T1198	prep	of,acids
R605	T1205	T1204	pobj	PAX6,of
R606	T1206	T1193	punct	", ",shown
R607	T1207	T1193	aux	has,shown
R608	T1208	T1193	auxpass	been,shown
R609	T1209	T1210	aux	to,act
R61	T246	T244	pobj	interactions,about
R610	T1210	T1193	xcomp	act,shown
R611	T1211	T1210	prep	as,act
R612	T1212	T1213	det	a,activator
R613	T1213	T1211	pobj	activator,as
R614	T1214	T1213	amod	transcriptional,activator
R615	T1215	T1216	punct	[,6
R616	T1216	T1193	parataxis	6,shown
R617	T1217	T1216	punct	],6
R618	T1218	T1193	punct	.,shown
R619	T1220	T1221	det	The,protein
R62	T247	T246	prep	of,interactions
R620	T1221	T1223	nsubj	protein,regulates
R621	T1222	T1221	compound	PAX6,protein
R622	T1224	T1223	advmod	directly,regulates
R623	T1225	T1226	det	a,range
R624	T1226	T1223	dobj	range,regulates
R625	T1227	T1226	amod	wide,range
R626	T1228	T1226	prep	of,range
R627	T1229	T1230	compound	target,genes
R628	T1230	T1228	pobj	genes,of
R629	T1231	T1232	punct	[,2
R63	T248	T247	pobj	PAX6,of
R630	T1232	T1230	parataxis	2,genes
R631	T1233	T1232	nummod	1,2
R632	T1234	T1232	punct	",",2
R633	T1235	T1232	punct	],2
R634	T1236	T1226	prep	including,range
R635	T1237	T1236	pobj	Pax2,including
R636	T1238	T1239	punct	[,15
R637	T1239	T1237	parataxis	15,Pax2
R638	T1240	T1239	punct	],15
R639	T1241	T1237	punct	", ",Pax2
R64	T249	T246	prep	with,interactions
R640	T1242	T1237	conj	Ngn2,Pax2
R641	T1243	T1244	punct	[,16
R642	T1244	T1242	parataxis	16,Ngn2
R643	T1245	T1244	punct	],16
R644	T1246	T1242	cc	and,Ngn2
R645	T1247	T1242	conj	glucagon,Ngn2
R646	T1248	T1249	punct	[,17
R647	T1249	T1247	parataxis	17,glucagon
R648	T1250	T1249	punct	],17
R649	T1251	T1223	punct	.,regulates
R65	T250	T251	amod	other,proteins
R650	T1253	T1254	det	The,gene
R651	T1254	T1256	nsubj	gene,has
R652	T1255	T1254	compound	Pax6,gene
R653	T1257	T1258	det	a,pattern
R654	T1258	T1256	dobj	pattern,has
R655	T1259	T1260	advmod	spatially,complex
R656	T1260	T1258	amod	complex,pattern
R657	T1261	T1259	cc	and,spatially
R658	T1262	T1259	conj	temporally,spatially
R659	T1263	T1258	compound	expression,pattern
R66	T251	T249	pobj	proteins,with
R660	T1264	T1256	prep	in,has
R661	T1265	T1266	det	the,eye
R662	T1266	T1264	pobj	eye,in
R663	T1267	T1266	punct	", ",eye
R664	T1268	T1266	conj	brain,eye
R665	T1269	T1268	punct	", ",brain
R666	T1270	T1271	amod	nasal,structures
R667	T1271	T1268	conj	structures,brain
R668	T1272	T1271	punct	", ",structures
R669	T1273	T1274	amod	spinal,cord
R67	T252	T242	punct	", ",known
R670	T1274	T1271	conj	cord,structures
R671	T1275	T1274	cc	and,cord
R672	T1276	T1274	conj	pancreas,cord
R673	T1277	T1278	punct	[,1
R674	T1278	T1256	parataxis	1,has
R675	T1279	T1278	punct	],1
R676	T1280	T1256	punct	.,has
R677	T1282	T1283	mark	Although,involved
R678	T1283	T1287	advcl	involved,emerging
R679	T1284	T1283	nsubjpass	PAX6,involved
R68	T253	T242	cc	so,known
R680	T1285	T1283	auxpass	is,involved
R681	T1286	T1283	advmod	clearly,involved
R682	T1288	T1283	prep	in,involved
R683	T1289	T1290	amod	multiple,processes
R684	T1290	T1288	pobj	processes,in
R685	T1291	T1290	amod	developmental,processes
R686	T1292	T1287	punct	", ",emerging
R687	T1293	T1294	amod	common,themes
R688	T1294	T1287	nsubj	themes,emerging
R689	T1295	T1287	aux	are,emerging
R69	T254	T255	nsubj	we,carried
R690	T1296	T1287	advmod	now,emerging
R691	T1297	T1287	prep	concerning,emerging
R692	T1298	T1299	det	the,role
R693	T1299	T1297	pobj	role,concerning
R694	T1300	T1299	prep	of,role
R695	T1301	T1300	pobj	PAX6,of
R696	T1302	T1299	prep	in,role
R697	T1303	T1304	amod	neural,tissues
R698	T1304	T1302	pobj	tissues,in
R699	T1305	T1287	punct	.,emerging
R7	T183	T182	prep	with,interact
R70	T255	T242	conj	carried,known
R700	T1307	T1308	nsubj	Gradients,are
R701	T1309	T1307	prep	of,Gradients
R702	T1310	T1311	compound	Pax6,expression
R703	T1311	T1309	pobj	expression,of
R704	T1312	T1308	acomp	important,are
R705	T1313	T1312	prep	for,important
R706	T1314	T1313	pcomp	determining,for
R707	T1315	T1316	amod	positional,characteristics
R708	T1316	T1314	dobj	characteristics,determining
R709	T1317	T1314	prep	in,determining
R71	T256	T255	prt	out,carried
R710	T1318	T1319	det	the,retina
R711	T1319	T1317	pobj	retina,in
R712	T1320	T1321	punct	[,18
R713	T1321	T1319	parataxis	18,retina
R714	T1322	T1321	punct	],18
R715	T1323	T1319	cc	and,retina
R716	T1324	T1325	det	the,neocortex
R717	T1325	T1319	conj	neocortex,retina
R718	T1326	T1327	punct	[,7
R719	T1327	T1325	parataxis	7,neocortex
R72	T257	T258	det	a,screen
R720	T1328	T1327	punct	],7
R721	T1329	T1308	punct	.,are
R722	T1331	T1332	nsubj	PAX6,plays
R723	T1333	T1334	det	a,role
R724	T1334	T1332	dobj	role,plays
R725	T1335	T1332	prep	in,plays
R726	T1336	T1335	pobj	development,in
R727	T1337	T1336	prep	of,development
R728	T1338	T1339	amod	specific,connections
R729	T1339	T1337	pobj	connections,of
R73	T258	T255	dobj	screen,carried
R730	T1340	T1339	amod	axonal,connections
R731	T1341	T1339	prep	between,connections
R732	T1342	T1343	det	the,retina
R733	T1343	T1341	pobj	retina,between
R734	T1344	T1343	cc	and,retina
R735	T1345	T1346	det	the,brain
R736	T1346	T1343	conj	brain,retina
R737	T1347	T1348	punct	[,18
R738	T1348	T1346	parataxis	18,brain
R739	T1349	T1348	punct	],18
R74	T259	T258	amod	systematic,screen
R740	T1350	T1341	cc	and,between
R741	T1351	T1341	conj	within,between
R742	T1352	T1353	det	the,forebrain
R743	T1353	T1351	pobj	forebrain,within
R744	T1354	T1355	punct	[,19
R745	T1355	T1353	parataxis	19,forebrain
R746	T1356	T1355	nummod	8,19
R747	T1357	T1355	punct	",",19
R748	T1358	T1355	punct	],19
R749	T1359	T1332	punct	.,plays
R75	T260	T258	prep	for,screen
R750	T1361	T1362	nsubjpass	It,involved
R751	T1363	T1362	auxpass	is,involved
R752	T1364	T1362	advmod	also,involved
R753	T1365	T1362	prep	in,involved
R754	T1366	T1367	det	the,differentiation
R755	T1367	T1365	pobj	differentiation,in
R756	T1368	T1367	prep	of,differentiation
R757	T1369	T1370	amod	neural,types
R758	T1370	T1368	pobj	types,of
R759	T1371	T1370	compound	cell,types
R76	T261	T260	pobj	proteins,for
R760	T1372	T1367	prep	from,differentiation
R761	T1373	T1374	amod	multipotent,precursors
R762	T1374	T1372	pobj	precursors,from
R763	T1375	T1367	prep	in,differentiation
R764	T1376	T1377	det	the,retina
R765	T1377	T1375	pobj	retina,in
R766	T1378	T1379	punct	[,16
R767	T1379	T1377	parataxis	16,retina
R768	T1380	T1379	punct	],16
R769	T1381	T1377	cc	and,retina
R77	T262	T263	dep	that,interact
R770	T1382	T1383	det	the,cortex
R771	T1383	T1377	conj	cortex,retina
R772	T1384	T1383	amod	cerebral,cortex
R773	T1385	T1386	punct	[,9
R774	T1386	T1383	parataxis	9,cortex
R775	T1387	T1386	punct	],9
R776	T1388	T1362	prep	through,involved
R777	T1389	T1388	pobj	activation,through
R778	T1390	T1389	prep	of,activation
R779	T1391	T1392	compound	bHLH,genes
R78	T263	T261	relcl	interact,proteins
R780	T1392	T1390	pobj	genes,of
R781	T1393	T1394	amod	such,as
R782	T1394	T1392	prep	as,genes
R783	T1395	T1394	pobj	Ngn2,as
R784	T1396	T1395	cc	and,Ngn2
R785	T1397	T1395	conj	Mash1,Ngn2
R786	T1398	T1362	punct	.,involved
R787	T1400	T1401	det	These,studies
R788	T1401	T1402	nsubj	studies,provide
R789	T1403	T1404	det	a,link
R79	T264	T263	prep	with,interact
R790	T1404	T1402	dobj	link,provide
R791	T1405	T1404	amod	clear,link
R792	T1406	T1404	prep	between,link
R793	T1407	T1408	compound	PAX6,function
R794	T1408	T1406	pobj	function,between
R795	T1409	T1408	prep	in,function
R796	T1410	T1411	det	the,retina
R797	T1411	T1409	pobj	retina,in
R798	T1412	T1411	cc	and,retina
R799	T1413	T1414	det	the,brain
R8	T184	T183	pobj	PAX6,with
R80	T265	T264	pobj	PAX6,with
R800	T1414	T1411	conj	brain,retina
R801	T1415	T1402	punct	", ",provide
R802	T1416	T1402	cc	and,provide
R803	T1417	T1402	conj	are,provide
R804	T1418	T1417	prep	of,are
R805	T1419	T1420	amod	particular,relevance
R806	T1420	T1418	pobj	relevance,of
R807	T1421	T1420	prep	to,relevance
R808	T1422	T1423	det	the,phenotypes
R809	T1423	T1421	pobj	phenotypes,to
R81	T266	T255	punct	.,carried
R810	T1424	T1423	amod	neurological,phenotypes
R811	T1425	T1423	prep	of,phenotypes
R812	T1426	T1425	pobj	individuals,of
R813	T1427	T1426	prep	with,individuals
R814	T1428	T1429	compound	PAX6,mutations
R815	T1429	T1427	pobj	mutations,with
R816	T1430	T1402	punct	.,provide
R817	T1432	T1433	nsubj	It,becoming
R818	T1434	T1433	aux	is,becoming
R819	T1435	T1433	acomp	apparent,becoming
R82	T270	T271	nsubj	We,used
R820	T1436	T1437	mark	that,act
R821	T1437	T1433	ccomp	act,becoming
R822	T1438	T1439	compound	transcription,factors
R823	T1439	T1437	nsubj	factors,act
R824	T1440	T1437	aux	do,act
R825	T1441	T1437	neg	not,act
R826	T1442	T1437	prep	in,act
R827	T1443	T1442	pobj	isolation,in
R828	T1444	T1437	cc	but,act
R829	T1445	T1437	conj	are,act
R83	T272	T273	compound	bioinformatics,techniques
R830	T1446	T1445	acomp	dependent,are
R831	T1447	T1446	prep	on,dependent
R832	T1448	T1447	pobj	interactions,on
R833	T1449	T1448	prep	with,interactions
R834	T1450	T1451	amod	other,proteins
R835	T1451	T1449	pobj	proteins,with
R836	T1452	T1453	aux	to,carry
R837	T1453	T1445	advcl	carry,are
R838	T1454	T1453	prt	out,carry
R839	T1455	T1456	poss	their,function
R84	T273	T271	dobj	techniques,used
R840	T1456	T1453	dobj	function,carry
R841	T1457	T1458	punct	[,21
R842	T1458	T1445	parataxis	21,are
R843	T1459	T1458	nummod	20,21
R844	T1460	T1458	punct	",",21
R845	T1461	T1458	punct	],21
R846	T1462	T1433	punct	.,becoming
R847	T1464	T1465	det	These,interactions
R848	T1465	T1466	nsubj	interactions,introduce
R849	T1467	T1468	amod	more,specificity
R85	T274	T275	aux	to,characterise
R850	T1468	T1466	dobj	specificity,introduce
R851	T1469	T1466	prep	into,introduce
R852	T1470	T1471	det	the,function
R853	T1471	T1469	pobj	function,into
R854	T1472	T1471	amod	regulatory,function
R855	T1473	T1471	prep	of,function
R856	T1474	T1475	det	a,factor
R857	T1475	T1473	pobj	factor,of
R858	T1476	T1475	amod	given,factor
R859	T1477	T1475	compound	transcription,factor
R86	T275	T271	advcl	characterise,used
R860	T1478	T1466	punct	.,introduce
R861	T1480	T1481	prep	To,described
R862	T1482	T1480	pobj	date,To
R863	T1483	T1484	advmod	only,interactions
R864	T1484	T1481	nsubjpass	interactions,described
R865	T1485	T1484	nummod	three,interactions
R866	T1486	T1484	nmod	PAX6,interactions
R867	T1487	T1488	nmod	protein,protein
R868	T1488	T1484	compound	protein,interactions
R869	T1489	T1488	punct	-,protein
R87	T276	T277	det	a,peptide
R870	T1490	T1481	aux	have,described
R871	T1491	T1481	auxpass	been,described
R872	T1492	T1481	punct	: ,described
R873	T1493	T1481	prep	with,described
R874	T1494	T1493	pobj	SOX2,with
R875	T1495	T1494	prep	on,SOX2
R876	T1496	T1497	det	the,element
R877	T1497	T1495	pobj	element,on
R878	T1498	T1499	npadvmod	lens,specific
R879	T1499	T1497	amod	specific,element
R88	T277	T275	dobj	peptide,characterise
R880	T1500	T1499	punct	-,specific
R881	T1501	T1497	compound	enhancer,element
R882	T1502	T1497	prep	of,element
R883	T1503	T1504	det	the,gene
R884	T1504	T1502	pobj	gene,of
R885	T1505	T1506	punct	δ,crystallin
R886	T1506	T1504	compound	crystallin,gene
R887	T1507	T1506	punct	-,crystallin
R888	T1508	T1509	punct	[,22
R889	T1509	T1493	parataxis	22,with
R89	T278	T279	advmod	highly,conserved
R890	T1510	T1509	punct	],22
R891	T1511	T1493	punct	;,with
R892	T1512	T1493	conj	with,with
R893	T1513	T1512	pobj	MDIA,with
R894	T1514	T1513	punct	", ",MDIA
R895	T1515	T1516	dep	which,modulates
R896	T1516	T1513	relcl	modulates,MDIA
R897	T1517	T1518	compound	PAX6,activity
R898	T1518	T1516	dobj	activity,modulates
R899	T1519	T1516	prep	in,modulates
R9	T185	T178	punct	: ,disrupt
R90	T279	T277	amod	conserved,peptide
R900	T1520	T1521	amod	early,development
R901	T1521	T1519	pobj	development,in
R902	T1522	T1521	amod	neuronal,development
R903	T1523	T1524	punct	[,23
R904	T1524	T1513	parataxis	23,MDIA
R905	T1525	T1524	punct	],23
R906	T1526	T1512	punct	", ",with
R907	T1527	T1512	cc	and,with
R908	T1528	T1512	conj	with,with
R909	T1529	T1530	compound	MAF,proteins
R91	T280	T275	prep	at,characterise
R910	T1530	T1528	pobj	proteins,with
R911	T1531	T1530	prep	on,proteins
R912	T1532	T1533	det	the,promoter
R913	T1613	T1614	nummod	two,hybrid
R914	T1533	T1531	pobj	promoter,on
R915	T1534	T1533	compound	glucagon,promoter
R916	T1614	T1611	compound	hybrid,technique
R917	T1615	T1614	punct	-,hybrid
R918	T1535	T1533	punct	", ",promoter
R919	T1616	T1601	cc	and,screened
R92	T281	T282	det	the,terminus
R920	T1617	T1601	conj	identified,screened
R921	T1618	T1619	nummod	three,proteins
R922	T1536	T1537	dep	which,causes
R923	T1619	T1617	dobj	proteins,identified
R924	T1620	T1619	amod	novel,proteins
R925	T1537	T1533	relcl	causes,promoter
R926	T1621	T1619	amod	interacting,proteins
R927	T1622	T1619	punct	", ",proteins
R928	T1623	T1619	appos	HOMER3,proteins
R929	T1624	T1623	punct	", ",HOMER3
R93	T282	T280	pobj	terminus,at
R930	T1625	T1623	conj	DNCL1,HOMER3
R931	T1626	T1625	cc	and,DNCL1
R932	T1538	T1539	amod	increased,expression
R933	T1627	T1625	conj	TRIM11,DNCL1
R934	T1628	T1601	punct	.,screened
R935	T1539	T1537	dobj	expression,causes
R936	T1630	T1631	det	The,interaction
R937	T1631	T1632	nsubjpass	interaction,disrupted
R938	T1540	T1539	prep	of,expression
R939	T1633	T1631	prep	between,interaction
R94	T283	T282	compound	C,terminus
R940	T1634	T1633	pobj	PAX6,between
R941	T1541	T1542	det	this,gene
R942	T1635	T1634	cc	and,PAX6
R943	T1636	T1637	det	these,proteins
R944	T1542	T1540	pobj	gene,of
R945	T1637	T1634	conj	proteins,PAX6
R946	T1638	T1632	auxpass	was,disrupted
R947	T1639	T1632	agent	by,disrupted
R948	T1543	T1542	amod	pancreatic,gene
R949	T1640	T1641	advmod	naturally,occurring
R95	T284	T282	punct	-,terminus
R950	T1641	T1642	amod	occurring,mutations
R951	T1544	T1542	compound	hormone,gene
R952	T1545	T1546	punct	[,17
R953	T1642	T1639	pobj	mutations,by
R954	T1643	T1644	npadvmod	C,terminal
R955	T1546	T1528	parataxis	17,with
R956	T1644	T1642	amod	terminal,mutations
R957	T1547	T1546	punct	],17
R958	T1645	T1644	punct	-,terminal
R959	T1646	T1642	compound	PAX6,mutations
R96	T285	T282	prep	of,terminus
R960	T1647	T1632	punct	.,disrupted
R961	T1548	T1481	punct	.,described
R962	T1550	T1551	advmod	Here,report
R963	T1552	T1551	nsubj	we,report
R964	T1553	T1554	det	the,results
R965	T1554	T1551	dobj	results,report
R966	T1555	T1554	amod	preliminary,results
R967	T1556	T1554	prep	of,results
R968	T1557	T1558	det	the,screen
R969	T1558	T1556	pobj	screen,of
R97	T286	T287	det	the,protein
R970	T1559	T1558	amod	first,screen
R971	T1560	T1558	amod	systematic,screen
R972	T1561	T1558	prep	for,screen
R973	T1562	T1561	pobj	proteins,for
R974	T1563	T1564	dep	that,interact
R975	T1564	T1562	relcl	interact,proteins
R976	T1565	T1564	prep	with,interact
R977	T1566	T1565	pobj	PAX6,with
R978	T1567	T1551	punct	.,report
R979	T1569	T1570	nsubj	We,used
R98	T287	T285	pobj	protein,of
R980	T1571	T1572	compound	sequence,alignment
R981	T1572	T1573	compound	alignment,algorithms
R982	T1573	T1570	dobj	algorithms,used
R983	T1574	T1573	cc	and,algorithms
R984	T1575	T1576	amod	secondary,structure
R985	T1576	T1577	compound	structure,prediction
R986	T1577	T1578	compound	prediction,programs
R987	T1578	T1573	conj	programs,algorithms
R988	T1579	T1580	aux	to,define
R989	T1580	T1570	advcl	define,used
R99	T288	T287	compound	PAX6,protein
R990	T1581	T1582	det	a,domain
R991	T1582	T1580	dobj	domain,define
R992	T1583	T1582	amod	new,domain
R993	T1584	T1582	prep	of,domain
R994	T1585	T1586	nummod	32,acids
R995	T1586	T1584	pobj	acids,of
R996	T1587	T1586	compound	amino,acids
R997	T1588	T1580	prep	at,define
R998	T1589	T1590	det	the,end
R999	T1590	T1588	pobj	end,at

PMC:1208879 JSONTXT 7 Projects

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PMC:1208879 JSON TXT 7 Projects